LOCUS       DAOPWC010000001        13787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_4534_length_13787_cov_3.157442, whole genome shotgun sequence.
ACCESSION   DAOPWC010000001 DAOPWC010000000
VERSION     DAOPWC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_13787_cov_3.157442"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKJ65_00005"
     CDS             complement(<1..627)
                     /locus_tag="PKJ65_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNZ40250.1"
                     /translation="MKRLKKIIIICGHYGSGKTNFAVNLAMDLRKQGYPVTLTDMDIV
                     NPYFRSSDHSEMLALKGIKVIAPVYANTNLDAPAIPAEMYSAFDGIGYSLFDMGGDDA
                     GATVMGQFFRNMKSKEYDMLYVINGKRAMIENVHDAAHMLYSIETASRLKATYIVNNT
                     HLKDLTDAKTILDSMEYAKKVSEAVHLPILCTTYPESLIKEQEISATGA"
     gene            780..1364
                     /locus_tag="PKJ65_00010"
     CDS             780..1364
                     /locus_tag="PKJ65_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ40251.1"
                     /translation="MKLEISEIAQRIRTLREIFDFTVEEMASATNVSVEDYIKLESGK
                     SSDYSFTFLYRCAEKFNVDIVEILTGENPHLGQYAIIRKGEGLPIKRREYFLYSHLAP
                     NFKNKTAEPFLVRAPYNEKEYNAPLKMNKHEGQEFDYILEGALKFKHGDREEILYAGD
                     SVYYNSGIEHGMTAALGKECVFLAIVMKDVSKER"
     gene            1364..3013
                     /locus_tag="PKJ65_00015"
     CDS             1364..3013
                     /locus_tag="PKJ65_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ40252.1"
                     /translation="MRNINLRYVDESYDEFGVLTSYRLKYPDNYNFGYDVVDDIAISD
                     PDRRALVWCNPEGEERIYTFADIKRLSDKTANYLRSLGIEKGDKVIVILKRHYQFWYT
                     SVALHKLGAVMIPVTFMITAHDAEYRINSASVKAVICTGEGHAAQAIDDVLNICPSLS
                     IRTMVYGHKEGWGDFDKGVQLADEHFERVDTHVKEPLLMYFSSGTSGYPKMVLHNHTY
                     SLAHLLTAKHWHNVHPEGLHLTIADTGWGKAVWGKLYGQWLMEAAVFAYDYDKFDAAE
                     ILSLIPKYKITTLCCPPTMFRFFIKEGLEKFDLSSLTYGTTAGEALNPDVFETWYKVT
                     GLKLMEGFGQTETVLAVCNLVNMQPKPGSIGKPSPLYEIDLLDADGNVCPTGITGEIC
                     IKTSNRYPDGLMDCYYRNKEMTDCTIYNGYYHTGDTAWKDEDGYLWYVGRNDDIIKSS
                     GYRIGPFEIESVLNSHPKVLESAVTGVPDEIRGQIVKATVVLSKGYSPSPELVKELQD
                     FVKHETAPYKYPRIIEFVENLPKTVNGKIRRVAIREQDNKK"
     gene            3148..3618
                     /locus_tag="PKJ65_00020"
     CDS             3148..3618
                     /locus_tag="PKJ65_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40253.1"
                     /translation="MRKSTAVKILISVMVVALALSLAGCGPKSRVKEAVELFIEKANA
                     LDYQGMFDLMATETVREIRGNKSLADFLKEKTSAEIAEAILKHILPAPVGDAEQFLKT
                     LSMEYEGADIREDFSSLYCVLKYEIDGETFEKDAVFNMLRKNVSEPWTVRDIAL"
     gene            3787..4323
                     /gene="thpR"
                     /locus_tag="PKJ65_00025"
     CDS             3787..4323
                     /gene="thpR"
                     /locus_tag="PKJ65_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNZ40254.1"
                     /translation="MRTFIAINFQQDVKDRLFNIERSLKSMSERGRFTPKDNLHLTLI
                     FLGEIAEDRVKLAQLCVKNISAPKFDMVVNRLGKFKRPDGDIYWAGIETNPVLTSIYS
                     QLYSNLSSYDFYIKDSEYVPHLTLARDYVLKDKEAMERYKENFQKISIHVESIHLMKS
                     EIIDRKLTYTVLYTKELD"
     gene            complement(4423..>4985)
                     /locus_tag="PKJ65_00030"
     CDS             complement(4423..>4985)
                     /locus_tag="PKJ65_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40255.1"
                     /translation="TIDPAEARNILRTAKQKKVPVSGGSGCKYSPQILELKKLVEQDA
                     FGRIYSGVLNFPASVESEYGGLYFYASHLVEMTLAVYGMDVKAISAFLNNGHITAIAH
                     YDKYDVTMQFANSKKYVSVIYGETDVDIRYLDMSGIFIAEFKDFVDMVRTGKSPLTDD
                     QLLIPVLVMNAIEKSLEQKKIVRIDR"
     assembly_gap    4986..4995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4996..5290)
                     /locus_tag="PKJ65_00035"
     CDS             complement(<4996..5290)
                     /locus_tag="PKJ65_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40256.1"
                     /translation="MFRVGMLGTENSHALAFAKEICFKEEYKDFKITAFYALEKAPSE
                     NIIEKCGITDAVIVDNPLEMMDLVDCVIVTNRHGKYHESCSLPFIEKGKPAFID"
     gene            complement(5305..6549)
                     /locus_tag="PKJ65_00040"
     CDS             complement(5305..6549)
                     /locus_tag="PKJ65_00040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ40257.1"
                     /translation="MKKQLALYGGEKVKQQPYGTGRRFGEEELTQLKEALDQNTLFYW
                     SGTKVKTLCAKFAKMYGAEHCVAASSGTAAIHVALGALGVTEGDEVITSPITDMGSII
                     GIMYQNAIPVFADLDPHTYNMDPKSIEKCITEKTKAIVVVHLAGNPAEMDEIMAIARK
                     HNIFVVEDCAQSFMCRYKGRLAGTIGDMGCFSLNDFKHISAGDGGLVLVKNPDLYVKT
                     FMFADKNYNRFSKGAALRVIEFLAPNYRMNELTAAVGIAQLDKLEKICATRKRYGEMI
                     TEGIKHLKGVYPHEVKKGNQSSFWFYMFRIDPKVVGTDTTTFAEALKAEGIGCQRGYI
                     PSVVYLYDMFQNKEGYPGTHAPFDSKYYGKDISYPKGLCPVAEEILDTAIRLNLNEFY
                     TEQDIQDIIDSIVKVTEYFKKG"
     gene            complement(6563..7345)
                     /locus_tag="PKJ65_00045"
     CDS             complement(6563..7345)
                     /locus_tag="PKJ65_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNZ40258.1"
                     /translation="MHIHIITDLEGISGVTDISQIDYDTDGYEYARVRLMADVNAAVR
                     GAFNGGADTVTVTDGHGRGKNFIDGALDKRAVNINLIELYKQGIHKQVDAVMFVGTHA
                     MPGTTGAFLDHVQSSVSWYNYYLNGVRCGELVQGGAFFGSYGAPVIFASGDEAACSEA
                     KHFFGDIQVAAVKKGIGRNKAEAYDFDLCEKIIESNAAAAISLVGKIKPYTVSKPVEI
                     IVDFTRTDYCDRAAERPGVERVDARRVIKRIPEITDYLSVLL"
     gene            7426..8976
                     /locus_tag="PKJ65_00050"
     CDS             7426..8976
                     /locus_tag="PKJ65_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNZ40259.1"
                     /translation="MRKRSFPKNFIKPLSLFLSLFICLNFLSICNSVTVIAAELSSVH
                     PDHVRGLYVRADIASLGLKEENVERYSEYVEALKEKKNSKIDISKINAFEKIVGLINA
                     TRLNAAVINVKDDQGYVTIPADSEYVLQAGSYNYQLKDIGKTMEQLKKYGIYTIARIV
                     VFKDGVLAQNASHAIKYDDGTVWKDYSGAAWVDPYDEWVRQYNIEVAKQAAIAGFDEI
                     QFDYIRFPEKFSKYDVSKKYLENKIRQDELIRQFLKDAYTQLKPYNVKVSVDVFGCAA
                     YLWDDQLNIDIGQVWYNLTQEADYISPMVYPSHYRGTNWYTQGDPNKHPYEVVKGAIE
                     DSLTINSAFEDKAGIRFWLQDFSMYDYEYGPLQIADQVKALHEKGIDTYMFWNNKNVY
                     DPYNYLILEDKHPKVIPDKYTVYDIENNNPADAAKRYLEADKNKDLYHSFILKAGKTR
                     QSEYADYVKNMQWSGITAYEFTGYRESFNTAQVFFSITKQGKQQKAVLFMLLEEGIWK
                     VHDYYIIR"
     gene            9052..9522
                     /gene="smpB"
                     /locus_tag="PKJ65_00055"
     CDS             9052..9522
                     /gene="smpB"
                     /locus_tag="PKJ65_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNZ40260.1"
                     /translation="MAKEDIKVISYNRKAGFDYELIEFYEAGIVLTGTEVKSLRAGAV
                     NMRDSYGYIKDYEAYVSGLHISHYDKGNINNVDPDRDRKLLLHKSEIRKLVLKMQQKG
                     YTLIPVKLYFVKNKVKLELSLAKGKKLYDKRDSIQKGEVEREIKRRMKDNHTGA"
     gene            9513..9864
                     /gene="ssrA"
                     /locus_tag="PKJ65_00060"
     tmRNA           9513..9864
                     /gene="ssrA"
                     /locus_tag="PKJ65_00060"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            10098..11147
                     /locus_tag="PKJ65_00065"
     CDS             10098..11147
                     /locus_tag="PKJ65_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ40261.1"
                     /translation="MRKFNYSDLKNKTWDSAIINRIGKIHEAKGRQELYLSQKPEELN
                     KLVELAKIQSTKASNAIEGIKTTDTRSKKIVNEKTTPKNRGEKEIAGYRDALNTIHES
                     FEYIPITPNYILQLHRILCGRDDEISYGGKFKNVQNYISATDAKGNSYTLFTPVSPFE
                     TPTAIDSLCNEFNKAINSHEVNPLLLIPIFIHDFLCIHPFNDGNGRMSRLLTTLLLYR
                     SGYYVGKYISLELKVSKLKDIYYDSLYASQIGWHEGKNDPTPFVSYLLSMIEMAYDDF
                     ESKVEIVSKKLSAYDTVKNALDVKIGKVTKSDVLELCPLLSASSIEKVLAKMVKEGKL
                     EKSGSGKNTFYIIKL"
     gene            11361..12051
                     /locus_tag="PKJ65_00070"
                     /pseudo
     CDS             11361..12051
                     /locus_tag="PKJ65_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            12217..12846
                     /locus_tag="PKJ65_00075"
     CDS             12217..12846
                     /locus_tag="PKJ65_00075"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNZ40262.1"
                     /translation="MKRCIIIGGAPICDYEYIRERVNKEDFFIYCDSGLKHKEKLGVS
                     PNLIIGDFDSHSNPYLDIETIVLPVMKDDTDTACAAKEAIKRGFQDIILCGVTGGRQD
                     HTLVNIFLLFRFDSYGIKARIIDDYAEMEVLSPGSTALIEDCYLYFSLVNMTGIARGI
                     TIKNAKYNLSNAKIESENQYATGNEVLPGKTAEVSIREGRVLLLKVRRE"
     gene            12916..13518
                     /locus_tag="PKJ65_00080"
     CDS             12916..13518
                     /locus_tag="PKJ65_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40263.1"
                     /translation="MDKIVLILIIIALLTVIVICFYRKNSAKSEQSDKSEFSNRGTDY
                     KNPGDLSRGIIYEPDMAKKPLPPFKEIAVVDNDDITIKITAADPYHMWGYSLHVYLEN
                     KSGGDTFYSFALSSAKVNGIQLKTDEVATDVNFYIELGKNYSYASGEIAFIAEDLKKK
                     GINKYGDIKNIELGFIVYETLNKDKVIFTGTVTVKLQRGN"
BASE COUNT         4273 a         2596 c         3017 g         3891 t
ORIGIN      
        1 tgctcccgtt gccgatattt cctgttcctt tatcagactt tcgggatatg ttgtacacaa
       61 tatcggtaaa tgtaccgctt ccgatacttt ctttgcatat tccatggaat caagtattgt
      121 cttagcatcg gtcaggtctt taagatgcgt attattcacg atatatgtcg ccttcaatct
      181 ggaggctgtc tctatggaat agagcatatg tgccgcatca tgcacatttt caatcatggc
      241 tctttttccg tttattacat aaagcatatc atattctttg cttttcatgt tgcggaaaaa
      301 ctgccccatt accgtggcac cggcatcgtc tcctcccata tcgaacaggg aatatcctat
      361 cccgtcgaat gccgaataca tttccgcggg tattgcggga gcatccaggt tggtgttggc
      421 ataaacgggg gctattactt ttatgccttt taaagccagc atttcactat ggtcggaaga
      481 gcggaagtag ggatttacta tgtccatatc ggttagtgtt acgggatatc cctgctttct
      541 taaatccata gcgagattta cggcaaaatt ggtttttccg cttccgtaat gtccgcatat
      601 gatgatgatt ttcttcagtc tcttcatatt acccgcacaa aaaattattt actccgtagt
      661 atatcattta tttttgttaa tttgaatata tatgtgtaaa aaagcgacgg aattcatttt
      721 aattgctttt gcatgaatat atatgtagaa taaagtttaa acagaacgga ggacttgtca
      781 tgaaactgga aatatcggaa atagcccaaa gaatcagaac actcagggag atatttgatt
      841 ttactgtaga ggagatggcg tccgctacca atgtatcggt agaagattat attaaattgg
      901 aaagcggaaa atcttccgac tattcattta ctttcctgta cagatgtgcg gaaaagttca
      961 atgtggacat agtggaaata ttgacagggg aaaatccgca tctcggacag tatgcgatta
     1021 taaggaaggg agaaggatta cccattaaga gaagagaata tttcttatac agccaccttg
     1081 ctcctaattt taaaaacaag accgcggaac cttttctggt aagagcgcct tataacgaga
     1141 aggagtataa cgcacctttg aagatgaata agcatgaagg tcaggagttc gactatatcc
     1201 tggaaggtgc actgaagttc aagcacggtg accgtgaaga gatattgtat gccggagatt
     1261 ctgtttacta caactccgga atagagcacg gtatgacggc ggctttgggg aaagaatgtg
     1321 tttttcttgc aatagtaatg aaagatgtaa gtaaggagcg gtaatgagaa atataaattt
     1381 acgatatgtt gatgaaagct atgacgagtt tggcgtgctg acaagttaca gacttaagta
     1441 tccggataac tacaacttcg gatatgatgt agtggatgat atagcaataa gcgatcccga
     1501 caggagagcg ctggtctggt gcaatcccga gggagaagag agaatatata cttttgccga
     1561 tataaagaga ctgtccgata agaccgccaa ctatttgcgg tcgctgggaa tagaaaaagg
     1621 cgacaaggta atagtcattc tgaagcgcca ttaccagttc tggtatacct cggtagctct
     1681 gcataagtta ggagcagtaa tgattccggt cacattcatg ataacggcac atgatgccga
     1741 atacagaatc aattcagctt cggtaaaggc cgtcatctgc acgggtgagg gacatgccgc
     1801 acaggctata gatgatgttc tgaatatttg tccctctctt tccatacgta ccatggtata
     1861 cggtcataaa gaaggctggg gcgatttcga caaaggtgtg caactggcgg acgagcattt
     1921 tgaaagagtg gacacgcatg taaaagagcc gctgcttatg tatttttctt cgggcacatc
     1981 cggttatccc aaaatggtac tgcataacca tacatattct ttagcacatc ttctgaccgc
     2041 aaagcactgg cataatgtcc atcccgaagg actgcatctt accatagccg ataccggatg
     2101 gggaaaagcc gtctggggca aactttacgg acagtggctg atggaagcgg cggtttttgc
     2161 atatgattac gacaagttcg atgcggcgga aatattgtcc cttataccca agtataagat
     2221 aacaacactc tgttgtcctc cgacaatgtt caggtttttc attaaagaag ggttggagaa
     2281 gttcgacctt tcctcgctta cttacggtac cacggcgggt gaggcattaa atccggatgt
     2341 gtttgaaacc tggtacaaag taacaggcct taaactcatg gaaggattcg ggcagaccga
     2401 aacggtactg gcggtgtgca atcttgtcaa tatgcagccc aaaccgggat ccataggaaa
     2461 accgtcgcct ctgtatgaga tagatcttct ggatgccgac ggcaatgtat gtcccaccgg
     2521 tattacgggt gagatatgca taaagacatc aaatcgttat cccgacgggt tgatggactg
     2581 ctattatcgc aataaggaaa tgaccgactg tactatttat aacgggtact accataccgg
     2641 cgataccgca tggaaagatg aggacggtta tttatggtat gtgggaagga acgacgatat
     2701 tataaagtca tccggttaca ggataggtcc gtttgagata gagagcgtgc tgaacagcca
     2761 tcccaaagta ctggaaagtg cggttaccgg agtgcccgat gagataagag ggcagattgt
     2821 aaaagcaacg gtagtgttgt caaagggcta cagtccttct ccggaactgg taaaagagct
     2881 gcaggacttt gtcaaacatg agaccgcacc gtataagtat ccgaggatta tagagtttgt
     2941 tgagaatctt cccaaaacgg ttaacgggaa aatacgaaga gtggcaataa gagagcagga
     3001 taataaaaaa tgaatggaaa aatatgatat tttatgtgaa aaatatactt tttgatgata
     3061 tactattgtt cgaaagattt ttttaatata ttatgatacg agttattcag taatgataaa
     3121 tacaatttgt attaagggag ttagaaaatg agaaaaagca cagcagttaa aattttaata
     3181 tcggtaatgg tagttgcttt ggcactttca ttggccggtt gcggacccaa atcaagagta
     3241 aaagaagcag tggagctttt tattgaaaaa gcaaatgcac tggattatca gggaatgttc
     3301 gatcttatgg caacggaaac tgtaagggaa ataagaggaa ataagtcgtt agcggatttc
     3361 cttaaagaga aaacatctgc ggagattgcg gaagctatac tgaaacatat tcttccggca
     3421 ccggtaggtg atgccgagca gttcttaaag acactttcta tggaatatga aggtgctgat
     3481 ataagagaag acttttcttc attgtattgt gttttgaaat acgagataga cggagagact
     3541 tttgaaaaag atgccgtctt caacatgctc agaaagaatg tatcggaacc ttggacagta
     3601 agagatatag ctctttaatt cggaccggtt ccttgcatag aggaatataa ctgaatagtc
     3661 tttaccgaaa aggtgcggac aggtttctta agaggataca atagaaggag gaaagtatga
     3721 ggctttcctc ttttttatgg aaagagaacg catcatttga tatactggta ttcaaggagc
     3781 acaactatga gaacatttat cgcaataaat ttccaacagg atgtaaagga ccgtttgttt
     3841 aacatagaga gaagtttgaa gagcatgtcg gaaagaggca gatttactcc caaagacaat
     3901 ctgcatctga cactgatttt tctcggagag attgcagagg acagagtaaa acttgcacag
     3961 ctgtgtgtaa agaacatatc ggcacccaag tttgacatgg ttgtcaacag gttagggaag
     4021 tttaaaagac ccgacggaga tatatactgg gcggggatag aaaccaatcc cgttctgact
     4081 tccatttaca gccagcttta cagtaattta agttcttatg acttttatat taaagacagt
     4141 gaatatgtgc cgcatcttac acttgcaaga gattatgtgc tgaaagataa agaagcgatg
     4201 gagaggtata aagagaattt ccagaaaata agtatacatg tggaaagcat tcatctaatg
     4261 aaatcggaga ttatagacag aaagcttacc tataccgttc tttatacaaa ggaactggat
     4321 taaaccgaat ataatgtaaa caacaaaacc gttaaggatt tttccggaaa gcacataaaa
     4381 agagcatcat acaagatgct cttttagatt ataaatcaat atctatctgt caattcttac
     4441 gatctttttc tgctccagag atttttctat ggcattcatc accaataccg gtatcagcag
     4501 ttggtcatcg gtaagaggcg atttacctgt tctgaccata tccacaaagt ctttgaattc
     4561 cgcaatgaat attcccgaca tatccaggta ccttatatcc acatcggttt ctccgtatat
     4621 gaccgaaacg tattttttgg aattggcaaa ctgcatggtt acatcatatt tgtcataatg
     4681 agcaattgcc gttatgtgcc cgttatttaa gaatgcggat atcgctttca catccattcc
     4741 gtaaaccgcc agtgtcattt ccacaagatg agatgcgtag aaataaagtc cgccgtattc
     4801 gctctcgacc gaagcgggga agttgagcac tccggaatat attcttccga aagcatcttg
     4861 ctccaccagt ttcttaagtt ccagtatttg cggtgagtat ttgcatcccg aaccgccgct
     4921 gacgggaact ttcttttgtt ttgccgttct taatatattt ctggcttccg ccggatctat
     4981 tgtaannnnn nnnnntgtct ataaatgcgg gttttccttt ttctataaaa ggcagtgaac
     5041 aggattcatg gtatttaccg tgcctgttgg ttactatgac acaatccacc aggtccatca
     5101 tttccaaagg attatccact attaccgcat cggtaatacc gcatttttct attatattct
     5161 ccgaaggtgc tttttccagc gcataaaaag ccgttatctt aaaatccttg tattcttcct
     5221 tgaaacatat ctcttttgca aaagcaagtg catgtgagtt ttccgttccc agcattccta
     5281 ctctaaacat tttgtgacct cctgttaacc tttcttgaaa tattccgtaa cttttactat
     5341 ggaatcgatg atgtcctgta tatcctgctc cgtatagaac tcattgagat tgagacgtat
     5401 agcggtatcc agtatttctt cggcaaccgg acacaatcct ttgggatagg atatgtcttt
     5461 tccgtaatac ttggaatcaa acggagcatg agtgcccgga tatccttcct tattctggaa
     5521 catatcataa agatatacca cggaaggtat gtatcctctc tggcatccta ttccctcggc
     5581 cttcaaagct tcggcaaatg tcgttgtatc tgtgcccact actttgggat ctattctgaa
     5641 catatagaac cagaaactgg actggttacc ttttttgact tcatgaggat aaacgccttt
     5701 taagtgttta attccctcgg ttatcatttc gccgtagcgt tttcttgtgg cacatatttt
     5761 ttccagttta tccagctgag ctattcccac tgcggcggta agctcgttca tcctgtagtt
     5821 gggagccaga aattctatta ctcttaaggc ggcgcctttg gaaaatctgt tgtagttttt
     5881 atcggcaaac atgaatgttt taacataaag gtcgggattt ttaaccagaa ccaatccgcc
     5941 gtctcccgcg gagatgtgct tgaaatcgtt gagactgaag cagcccatat cacctatggt
     6001 tcccgccaga cgtcctttgt atctgcacat gaagctctga gcacagtctt ccacaacaaa
     6061 aatgttgtgc tttcttgcga ttgccattat ttcatccatt tcggcaggat ttcccgcaag
     6121 atgcaccact acaatggctt tggtcttttc ggtaatgcat ttctctatac tcttgggatc
     6181 catattgtag gtatgcggat ccaggtctgc aaacacggga atggcattct gatacataat
     6241 ccctattatg cttcccatgt cggtgatagg ggaggttatg acctcgtctc cttccgttac
     6301 acccaaagca cctaaagcta catggatggc tgcggtaccc gacgatgctg ccacgcagtg
     6361 ttccgctccg tacattttgg cgaacttggc acataatgtc ttgactttgg ttcccgacca
     6421 gtagaacaat gtgttctgat ccagtgcttc tttgagctgg gtaagttctt cttccccgaa
     6481 tcttcttccc gtaccgtaag gttgctgttt tactttctcg ccgccgtata aggcaagttg
     6541 ttttttcatt cagtttttcc tcctatagta atactgataa ataatcggtt atttccggta
     6601 ttctctttat aactcttctt gcgtctactc tctccacacc cggtctctcg gcagctctgt
     6661 cacaataatc ggtccttgtg aaatccacta ttatttccac cggcttggag acggtatatg
     6721 gctttatctt gcctacaagc gatatggcgg ctgccgcatt tgattcaatg attttttcgc
     6781 ataaatcaaa gtcatatgct tccgctttat ttctccctat gcctttttta acagcggcaa
     6841 cctgtatgtc tccgaagaag tgtttggctt cactgcatgc cgcctcatca cccgatgcga
     6901 atattaccgg tgcgccgtat gagccgaaaa aagctccgcc ctgcacaagt tctccgcatc
     6961 gcacaccgtt taaatagtag ttgtaccagc ttacggaaga ctgtacatgg tctaaaaatg
     7021 cacctgttgt accgggcatg gcatgagttc ccacaaacat aacggcatcc acctgtttgt
     7081 ggataccttg tttatacagt tcaatcagat ttatatttac agctctttta tccaatgctc
     7141 cgtcaatgaa gtttttgcct cttccgtgac cgtcggtaac cgttacggta tccgctcctc
     7201 cgttgaaggc tcctcttact gcggcattga catccgccat aagtcttacc ctggcatact
     7261 cgtaaccgtc ggtatcataa tcaatttggc ttatatccgt tactccgctt ataccttcca
     7321 aatccgtaat gatatgtatg tgcatattta ccttaaatcc cttcttaagg tattatacca
     7381 caggaatatg ttcataaaca ctaaacatat cggtataatg tatgtatgcg taagaggtca
     7441 tttccgaaaa actttataaa gccgctttct ctgttcttat ccctgtttat atgcttaaac
     7501 ttcttaagca tatgcaactc ggttacggta atagctgcgg aattaagctc cgtgcatccc
     7561 gatcatgtga gaggacttta tgtcagagcg gatatagcat ctttgggact gaaagaagaa
     7621 aatgttgaaa gatactctga atatgtagaa gcgctgaaag aaaagaaaaa ttcaaagatt
     7681 gatatcagta agataaatgc atttgaaaag atagtaggcc ttattaatgc aacaaggtta
     7741 aatgccgcgg ttatcaatgt taaagacgat caaggctatg tcaccatacc tgccgattcg
     7801 gaatatgtgt tgcaggcggg aagttataac tatcagctta aagatatagg taagaccatg
     7861 gaacagctaa aaaagtacgg tatatataca atagccagga ttgtggtgtt caaagacggt
     7921 gttctggcac aaaacgcttc tcatgcaata aagtatgatg acgggacggt ctggaaagac
     7981 tattccggtg cagcctgggt ggacccgtat gatgaatggg tacggcagta taacatagag
     8041 gtggcaaaac aggccgcaat tgcgggcttt gacgaaatac agtttgacta tatccggttt
     8101 ccggaaaagt tctcaaagta cgatgtgtcg aaaaaatacc tggagaataa aatacggcag
     8161 gatgagctga tacggcagtt cttaaaagat gcatatacgc agcttaagcc gtataatgtg
     8221 aaagtatccg tcgacgtgtt cggttgtgcg gcatatcttt gggatgacca actgaacata
     8281 gatatcgggc aggtgtggta caaccttacc caagaggcgg attatataag ccctatggta
     8341 taccccagcc attacagagg tacaaactgg tatacgcaag gcgaccccaa caagcatccg
     8401 tatgaagtgg taaaaggtgc gatagaagac tctttgacaa tcaattcggc atttgaagac
     8461 aaggcgggaa taagattctg gttgcaggat ttttccatgt acgattatga gtacgggccg
     8521 cttcagattg cggaccaggt caaagctttg cacgagaagg ggatagatac atatatgttc
     8581 tggaataaca agaatgtgta tgacccgtat aattatctta tacttgagga taaacatcct
     8641 aaggtaatcc cggacaaata taccgtttac gatatagaaa acaacaatcc tgccgatgcc
     8701 gcaaaaagat atctggaagc cgataagaat aaagatttgt atcactcctt tatattaaaa
     8761 gcgggaaaaa cccggcagag cgagtatgcc gactatgtta aaaacatgca gtggtcggga
     8821 attaccgcat atgagtttac cggttacaga gaaagcttca atacggcaca ggtgtttttc
     8881 agcataacaa agcagggcaa acagcagaaa gcagtactgt ttatgctctt ggaagaaggt
     8941 atctggaaag tgcacgatta ttacattata cggtaaagaa tattaagaat ttgtaacaat
     9001 taacaataaa ttcaattata tggtataatt tgcttccgaa ggagagtgga cttggctaaa
     9061 gaggacataa aagtcatttc gtataacaga aaagccggtt ttgactacga gcttattgag
     9121 ttttacgagg cgggaatcgt gcttacaggt accgaagtga agtctttaag agccggagcg
     9181 gtaaatatgc gtgattctta cgggtacata aaggattacg aggcatatgt atcggggctg
     9241 cacatctccc attatgataa gggaaacata aacaatgttg accccgatcg tgacagaaaa
     9301 ttgctgttgc acaagagcga gataaggaaa ctggttctga agatgcagca gaaagggtat
     9361 acccttattc ccgtaaagtt gtactttgtg aaaaacaagg taaaactgga actgtctttg
     9421 gctaaaggaa agaaactcta tgacaagaga gattccatcc agaagggcga agtcgaaagg
     9481 gaaataaaaa gaagaatgaa ggacaatcat acgggggcgt aaaggtttcg acgggatgat
     9541 tgaaatattg acggcatgca gaggattctc gttggcctct ttaaaaaacg ggagattaaa
     9601 ataaacgcaa aacctaattt tgcaccttta gcagcttaag ctggctaaac atcccaccgg
     9661 aaatcacggg gtccggaccg gggtgtcatc taccgtgaaa acccgataat caaagctttg
     9721 aggttaaagg gtaaactatg aagctaccga taaagcaccc ggcttacagg ggactttaaa
     9781 gggaaattaa acagtaagct acgcatgtag aagtcggtac ggatattgtt tcggacgagg
     9841 gttcgattcc ctccgcctcc accattataa tatgaacaag tcgatactaa aaatatcggc
     9901 ttttttcttt ttatacggat aaatacagca aatctatatt tgattttatt ctaaacttgg
     9961 ctttcagtca tagtcgcacc tccggtgtgt aaatgtcact ctaaaagaga attcaagtat
    10021 ataagagaaa aagattctcg taatttttga ctattctctc aatttgatac ataatttacg
    10081 agaaaggaat ttagtttatg agaaagttta attattcgga tttaaaaaac aaaacatggg
    10141 attctgctat aataaataga atcggtaaaa tacatgaagc taagggtaga caggaattat
    10201 atttatctca aaaaccggaa gaattaaata aattagtaga acttgctaaa atacaaagta
    10261 caaaagcaag taacgcaatt gaaggaatta aaactactga tacaagatca aaaaagattg
    10321 taaatgaaaa aacaactcca aagaacagag gcgaaaaaga aattgccgga tatagagatg
    10381 cactgaatac aatacatgaa agttttgagt atattcctat tactccaaat tatatattac
    10441 aattgcatcg aattttatgc gggcgagatg atgaaatatc atatggcggt aaatttaaaa
    10501 atgttcagaa ttatataagt gcaactgatg ccaaaggcaa ttcatataca ttgtttacac
    10561 ctgtatctcc ttttgaaacg cctacggcga tagacagttt atgtaatgaa tttaataaag
    10621 caataaatag tcatgaagtt aacccgcttt tattaattcc tatatttata catgatttct
    10681 tatgtattca tccttttaat gatggaaatg gcagaatgag cagattatta acgacattac
    10741 tattatatcg ttcaggatat tatgttggaa aatatatttc attagaattg aaagtatcaa
    10801 aactaaagga tatatattat gattcattat atgcttctca aatcggatgg catgaaggaa
    10861 aaaatgatcc tacaccattt gtaagttatc tattaagtat gattgaaatg gcatatgatg
    10921 attttgaaag taaagtagaa attgtatcta aaaaactaag tgcatatgat acagtaaaga
    10981 atgcgttaga tgtgaaaatc ggaaaagtta caaaatctga tgttttagaa ctatgcccat
    11041 tactttcggc aagttctatt gaaaaagtat tagccaagat ggtaaaagaa ggaaagctcg
    11101 aaaagagtgg ctccgggaaa aatacattct atatcataaa attataactt tctctcattt
    11161 taggcgactt tttgcgagaa tgtaatggag tttgggagaa agactaaaag aactatggac
    11221 aggtgtgcta ctattgcaga cacagattgg aaaaatgttc agcgccggac agtctaatat
    11281 tttcaaatat gagctcggca ctaccatgaa caagagattt aaggaaacac taaaagaaat
    11341 gatgtaggag gaaagcgaaa atgtcggaaa acgataaggt tcagcttttt gaaaatcaaa
    11401 aaatccgtac cgcatgggac aaggaaaaag aagaatggta tttctccatt gtagatgtgg
    11461 taggtgtatt gaccgatcag ccggacaccc gccgtgccgc caaatattgg agcgtcctga
    11521 aaacgcgcct gaaaaaaaat ggaagcgagt tgcctacaaa atgtagtcaa ctgaaaatgc
    11581 tctctgcaga cggtaagcgt tacaagaccg atgttgccaa caccgagcag cttcttcgca
    11641 ttatccagtc gattccatcc ccaaaagcgg aaccttttaa gttgtggctt gcacaagtcg
    11701 gcagagagcg catagaagaa accattgatc ctgaacttgc cgttaaccgc gcattggaga
    11761 cttatcagaa aaagggttac agcgacgaat ggattcatca gcggattctt tctatccgag
    11821 tccacctcaa cggagatttc caagaatgag aatccaggct cttttgaaga gagccatgca
    11881 attgcaaagc gtggtggtac agcggcaaaa gaagccaggt ctgctgttga gaagcagacg
    11941 ggaaagccgg tgattacaaa ccggaacgca gcacagctca acgccgttgt aacgaatctg
    12001 attgaaaaca ccgacgacat cattgacaca gacggaggtg gtgacaaatg aatgccgtaa
    12061 tctcggcttt gaaagcaatg tctccaccgg ataaggtgaa attatatttt acggtcctac
    12121 ttattatcct atcggcatca cttatgcaat tgttttaatt gccctcgggt gatatgattt
    12181 ggatagcaaa aacagtccgt aagatgaggt gttcaaatga aaagatgtat tatcatcggc
    12241 ggtgcaccga tttgcgatta tgaatatata agggaacggg tgaataaaga agattttttc
    12301 atctactgcg acagcggatt gaaacataag gaaaaactcg gcgtatcacc gaatctgata
    12361 atcggagatt ttgattctca cagcaatccg tatctggata tcgagaccat tgttttgccc
    12421 gtaatgaagg atgacacaga tacggcttgt gctgccaaag aagcaatcaa aagaggtttt
    12481 caggatatta tcctttgcgg tgttacaggc gggcgtcaag accataccct tgtgaatata
    12541 ttcctgctgt tcagattcga cagttacggt atcaaagccc gtattattga cgattatgcc
    12601 gaaatggaag tgctttcacc gggaagcacg gcattgattg aggattgcta tctgtatttt
    12661 tctctcgtaa atatgaccgg aatcgcacgt ggtatcacga taaaaaatgc caaatacaat
    12721 ttaagcaatg caaagattga aagcgagaat cagtatgcta ccggtaatga agtgctgccc
    12781 gggaagacgg cggaggtgtc aatcagggag gggagagtat tacttttgaa ggtgagaaga
    12841 gaataacagg attgggaata gattgtttac aggtttgttt gaatatctga taccgatttc
    12901 atataggagg aacagatgga taagatagta ttaattttga ttataatagc attactgact
    12961 gtaattgtga tttgctttta caggaagaat tctgctaaat ccgagcaatc cgacaaatcg
    13021 gaattcagca acagaggtac cgattacaaa aatcccggtg atttatcacg cggtataatc
    13081 tatgagccgg atatggcaaa aaagccgctg ccgcctttca aggaaatcgc agtagttgac
    13141 aatgatgata tcactattaa aatcacagct gcggatccct atcatatgtg gggttactca
    13201 ttacatgtgt atctggagaa taaatcgggc ggcgatacct tctactcatt tgctttatca
    13261 agcgcaaagg tcaacgggat ccaattaaaa actgatgaag tcgcaaccga tgtcaacttc
    13321 tacatagagc ttggcaagaa ctacagttat gcaagcggcg aaatcgcatt tatagccgag
    13381 gatcttaaga aaaagggaat aaataagtat ggagatataa agaatattga gttaggcttt
    13441 atagtctatg aaacgctgaa taaagacaag gttattttta ccggtacggt aacggtaaaa
    13501 cttcagagag gtaattaatt acgatgggtt tttgcagtta cctgtctgtt gttgacaggc
    13561 ggttgtacta cccgaaggct accgatttat atattttaca cattgaaaga gttagtgcaa
    13621 tttcaatcgg attatcgtga aacactatct ttagataaag tcttggacaa aaccccttcg
    13681 ttttatcaag taacttccaa gtatgacagg aagttttttg aagaccgctc gctaataccg
    13741 gtttatgcaa taaaaacagg gattctgata tatgttttca tattaat
//
LOCUS       DAOPWC010000002        12547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_5113_length_12547_cov_4.242395, whole genome shotgun sequence.
ACCESSION   DAOPWC010000002 DAOPWC010000000
VERSION     DAOPWC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5113_length_12547_cov_4.242395"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..359)
                     /locus_tag="PKJ65_00085"
     CDS             complement(9..359)
                     /locus_tag="PKJ65_00085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ40264.1"
                     /translation="MTDILRILFSAIAPVAALIILLLVIDRHDREPAKLLIQQFVAGC
                     LISIPAIFIENFLEGLNIFSGTILGVAYTSYIVAGLTEESLKRASVYLVSYHKPAYNE
                     KLDGIIDSVNLFGD"
     gene            complement(368..937)
                     /locus_tag="PKJ65_00090"
     CDS             complement(368..937)
                     /locus_tag="PKJ65_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ40265.1"
                     /translation="MMIYLELFWEFFKMGLFSVGGGLATIPFLYDIGRRYDWLDTAVI
                     PDMIAISESTPGPIGINMATYTGYTAAGIPGALIASLAIILPSAIIVLLISGFLASFG
                     RDKRVLNTMYGLRPASLGLITAAAFMLFKVSVITYDAFVESGNILDIIDIKALVLFVC
                     LLVIRLFFRKIHPILFIVFAGAIGIIFNF"
     gene            complement(921..1484)
                     /locus_tag="PKJ65_00095"
     CDS             complement(921..1484)
                     /locus_tag="PKJ65_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ40266.1"
                     /translation="MRILLELYWIFFKVGLFTFGGGYTMLPLLQKEVVEKKKWLTSEE
                     IIDFYTLSQCTPGIIAVNVSTYIGFKKKRFWGGIISALGVATPSIIVITVIAAVLTNF
                     LHIKEVAYAFTGIRVAVTALVIVTIVDMYKKSVVDWLCIILYIASFLLSVLTSISPVF
                     IVLGAGITGLVLGTRMRERKGDNDDIS"
     gene            1752..2633
                     /gene="dapF"
                     /locus_tag="PKJ65_00100"
     CDS             1752..2633
                     /gene="dapF"
                     /locus_tag="PKJ65_00100"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNZ40267.1"
                     /translation="MKFTKMHGAGNDYIYVDCMECKIDNPSSLAIKLSDRHKGIGSDG
                     LVLIQPSKTAHFYMRMFNSDGSEGAMCGNAIRCVAKYVYDNKLTDKLKLDIETKSGIK
                     HLKLFPDKKGKIDSVEVDMGIAYLKRKEIPMVYDNSNREETYINKPLVVDGKKYNVTC
                     VSMGNPHCVIFMNDKDNKSNLMQSLDKLDLDNIGPDFERHSYFPDRINTEFVEIIDRN
                     TLKMRVYERGSGETMACGTGACATVVAAVLNGICERNSEVSVILRGGTLKIIYSDDGR
                     VIMKGEAVHVFDTEINI"
     gene            2654..3832
                     /locus_tag="PKJ65_00105"
     CDS             2654..3832
                     /locus_tag="PKJ65_00105"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ40268.1"
                     /translation="MAKPNVNYGNLKQSYLFRDIATKVAAYKKAHPDRKVISLGIGDV
                     TLPLAKKVVEALKAASEEMGKAETFRGYAPDQGYDFLREKISEHYKKRNVTVDISEIF
                     VSDGAKSDLGNITEIFSRDNIVMIPDPVYPVYMDTNVMDGRKIIFADANKENHFLAMP
                     DKRHADMIYLCSPNNPCGSVYSKAQLEKWVQYALKHNSVILFDAAYEAFITDDKLPRS
                     IFEIEGAEKCAIEFCSFSKLAGFTGTRCAYTVIKKELILEGFSLRDMWVRRQSTKLNG
                     VSYIIQRGAEAVFTEEGMKQCKDNIRYYLDNAKLISALLTEKGIYHTGGNNSPYIWMS
                     CPNNLKSWEFFDLLLNETCVVGTPGSGFGHNGEGYFRLTSFGSRENTIEAVERLRKVL
                     "
     gene            4007..6394
                     /locus_tag="PKJ65_00110"
     CDS             4007..6394
                     /locus_tag="PKJ65_00110"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNZ40269.1"
                     /translation="MEEFEKRYDGLAVEKEMQRFWEDNNIYKFVPDVSRPLYSIDTPP
                     PTVSGSLHIGHIFSYTQAEMIARFRRMQGYNVFYPFGFDDNGLPSERLVEKETGIKAH
                     DIPRSQFQKSCIEITKKYEDEFMNLWKSMGFSCDWNLQYTTISSNTQRLSQKSFLELA
                     RAGHAYIKESPVLWCTECQTSIAQAELDSKEIETYFHYIPFGEGKITVEIATTRPELL
                     YGVVCVFVNPEDNRYSDLIGKTIKVPLYGFEVPILADEKVAIDKGTGAVMCATFGDTT
                     DVEWVTQKGLEYKKVILSDGTIASDVPFIAGMKVKEARRKIVELLEEKRVLLKSEKLT
                     HIVSVHERCGTEVEIIPSRQWYIDVLSKKEELLVAGDRVNWYPEHMQNRYIDWVENLK
                     WDWCISRQRYFGIPIPVWYCKNCGKPVFAEYEQLPVNPMETNYSGKGCDCGCNEYLPD
                     KAVFDTWATSSITPQLNCDKAAEFGINEGFIPMSMRTQAHEIIRTWAFYTIVKSLYHT
                     DDIPWSDIMICGFVLAKPGEKISKSKDNSKLSPRELINQYSADAIRYWAANARLGIDT
                     YFDIQEMRDASNRLMTKLWNSAKFVLSHLTDYNPKYVPEYLLPIDKWIIERTNEAILD
                     VTKWLNEYEIGLARKIVDDLFWKDLCDYYIEIVKERLYRPDIHGVKERKSAQHAIYYC
                     LLNVLKMYSIYIPHETEYIYLKGFKDFVGIKSIHLTQWEKPVSVDKSIIEFGETVKDS
                     IAEVRKFKSENNLSMRSEIDEAVINYRRAHFDRLKETEKDFIACTHVKKVSYKLI"
     gene            6578..7777
                     /locus_tag="PKJ65_00115"
     CDS             6578..7777
                     /locus_tag="PKJ65_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ40270.1"
                     /translation="MSYSGGVIIEGAKWVGKSTTAGIFSNTVIKLQDPLVKKQYQILA
                     TISKDEVLKGEKPILFDEWQEVPEIWDFIRLDIDENNYKGAYLLTGSTKKQNITTSHT
                     GTGRINSVYMRPMSLYETGESNGLISLKELFSQPPLVPVKSNITIDNIAHCICRGGWP
                     GSLDLNKDLQLEIPKDLLESIIKRDIDEVDGITKDKEKFRKIIKSYARNISTLAANST
                     IYEDQSYEVIVNPKTFDTYMNSLKRLYIIEDVLPWSQNIRSSTRLRKGTKRQFVDPSI
                     AVAALGLSPDKLKNDFETFGFLFESIATRDIRVYAEEIGGTVYYYNDSTGLEVDLIVE
                     LNDGRWGGIEVKLGENEADKASANLIKLANISATKPSFLMLLTNTQMAYQRDDGVYVI
                     PLGCLRP"
     gene            7914..9242
                     /locus_tag="PKJ65_00120"
     CDS             7914..9242
                     /locus_tag="PKJ65_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40271.1"
                     /translation="MFKRKIYDKLLEWKETSKGRTALLIEGARRVGKSTIVTEFGKNE
                     YKSYILIDFAFAPTGVKELFNDISDLDYLFLQLQLQFKADLHERNSLIIFDEVQFCPK
                     ARQAVKKLVEDGRYDYIETGSLISIKKNVEHILIPSEERKIEMFPMDFEEFLWAIGDN
                     TTNNILRKFYNEKISPGQDQNRALMRKFRLYMLVGGMPQAVKAYIETNNFKFVDEVKR
                     DIIQLYEEDFYKIDSSGKISMLFDSIPSELSRHSSRYQVSSVLKNQRAATISEEIAEL
                     VASKTVLISYHSNDPSVGMTTNLSMEHFRLFMADTGLMVTCMFKDKDFTDNIIYEKLL
                     NNKLSKNLGVLYENIVAQTLAFNGNKLFYYTFYDETQKRSFEVDFLLSKDNKVSPIEV
                     KSSNYRTHRSMDEFANKYSNRINTKYVIHTQDLKKNGNLIYLPFYLAQFI"
     gene            complement(9253..10407)
                     /locus_tag="PKJ65_00125"
     CDS             complement(9253..10407)
                     /locus_tag="PKJ65_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ40272.1"
                     /translation="MIKPFETVLPVGKLSIIGMRGCEEITKKVDWYLKQRAGYKDSDH
                     TFITEAICPRFGTGEAKGVILESIRGHDLFIICDVFNYGVTYEMYGMQVPMSPDDHYA
                     DLKRIIAATNGKARRITVIMPMLYEGRQHKRKYRESLDSAISLQELVGMGVENIITFD
                     AHDPRIQNAIPLNGFESVPVAYQMMKKLTASFPDIIFDKEHTLVISPDEGGMNRAIPF
                     ATMIGLNLGMFYKRRDFSVVEQGRNPIISHEFLGDSVEGKDVIVVEDIISSGESALDI
                     FYQLTKMKAKRIFMCCTFGLFCNGLEAFDKAYEEGAVTGVLTTNLVYRSPELLKRPWF
                     KEVDMSKYMSFIIDTLHHDASMSNLLNPVDRIDKLMEKVKSRQLEEGLIK"
     gene            complement(10445..11845)
                     /gene="gltA"
                     /locus_tag="PKJ65_00130"
     CDS             complement(10445..11845)
                     /gene="gltA"
                     /locus_tag="PKJ65_00130"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNZ40273.1"
                     /translation="MAKINMTPEKTKMPEQNPDIRNRNFLEVATGYTPEMAQEEAMRC
                     LNCKHKPCVSGCPVNVDIPAFIQSVAQGDIEKAYEIITKTNSLPAICGRVCPQETQCE
                     LPCVRGIKGEPVAIGRLERYVADYMLAKETFIADTPKPTVKKNKKVAVIGSGPAGLTA
                     ASDLCDMGYDVTIFEALHRAGGVLVYGIPEFRLPESIVEKEIERLKAKGVCIELNMVI
                     GKTISIQELHEEEGFEAIFVGTGAGLPNFMNIPGENLNGVFSANEFLTRVNLMKAYDP
                     SYDTPIYFGSHVAVVGGGNVAMDAARAAKRLGAEQVYIVYRRSEQEMPARAEEVHHAK
                     EEGIEFRLLTNPTKILGETGWVTGMECVRMQLSEPGPDGRRRPVVIPGSEYVLKTTSV
                     IMAIGTSPNPLIKSTTEGLETNKWGCIVADEETGQTSIPYIFAGGDAVTGAATVILAM
                     GAGKKAAAAIDKYLDK"
     gene            complement(11845..>12547)
                     /locus_tag="PKJ65_00135"
     CDS             complement(11845..>12547)
                     /locus_tag="PKJ65_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ40274.1"
                     /translation="ERIPLTISDYNREEGWVSIIFQKVGKTTYLLDTLQQGDYILDFV
                     GPLGIASHTDNMSRVCVVGGGLGCAIAYPQAKKLFGMGLHVDIIAGFRNKSLVFLKEE
                     MKHNSTNYYLMTDDGSAGEKGLVTKKLKELIEGGANYDTVIAIGPVVMMKYVSLLTKE
                     HNIKTIVSMNPIMIDGTGMCGGCRLTVDGQTKFACVDGPDFDGHKVDFDGIMQRLSMY
                     KEQEKHKCNLFRGIE"
BASE COUNT         3939 a         2352 c         2611 g         3645 t
ORIGIN      
        1 ttattctatc aatccccaaa caagtttaca ctatctataa ttccgtccag tttttcattg
       61 taagcgggtt tatgatagga tacaagatat accgatgctc ttttaagcga ttcctccgtg
      121 agacccgcta ctatgtagga ggtatatgct actcccagaa tggttccgga gaatatgttc
      181 agtccttcca gaaaattttc tataaaaatt gccggaatac ttattagaca tcccgcaaca
      241 aactgttgga ttaacagttt tgcgggctct ctgtcatgtc tgtcaattac cagaagcagt
      301 atgataagtg ctgcaacagg tgcaatagcg gaaaaaagta ttctgagaat atcagtcata
      361 agtcccccta aaaattaaat attatcccga ttgctccggc aaagactata aacaatatcg
      421 ggtgtatttt tctgaaaaac agtcttatta ccagtaaaca aacaaacaat accagagcct
      481 ttatatcgat tatatcaaga atatttcccg attcaacaaa agcatcatat gttatgaccg
      541 aaactttgaa aagcataaaa gccgccgccg ttattagtcc caaagaagcg ggtcgcaatc
      601 cgtacattgt attaagaact ctcttgtccc gaccgaaaga agccaaaaac ccggatataa
      661 gaaggacaat gatagcggaa ggaagaatga ttgccaggga tgctatcaat gcaccgggaa
      721 taccggcagc ggtgtagccc gtataagttg ccatgtttat tcctatggga cccggagtgg
      781 actcggatat agctatcata tcggggatta cggctgtgtc cagccagtcg tatctccttc
      841 cgatatcata aaggaaaggt attgttgcaa gacctcctcc tacggagaaa agacccatct
      901 taaaaaattc ccagaacagt tcaagatata tcatcattat ctccttttct ctcccgcatt
      961 cttgttccca gaaccagtcc ggtaatgccg gcacccaata ctataaatac cggagatatt
     1021 gaagtaagta ccgataaaag gaaggatgcg atgtacagta tgatgcaaag ccagtccaca
     1081 accgactttt tatacatatc cactattgta actataacaa gtgccgtaac ggctactctt
     1141 attccggtaa aggcgtaagc gacttctttt atgtgtaaaa aatttgtaag aaccgccgct
     1201 attaccgtta taactataat cgacggagtt gccaccccca aagcactgat tatccctccc
     1261 cagaaccgtt ttttcttgaa acctatataa gtagacacat taacggcgat aatgcccggc
     1321 gtacattggc taagggtata gaagtcaatg atttcctcgg aagtgagcca cttctttttc
     1381 tccacaacct ctttttgaag aagaggcagc attgtataac ctccaccgaa ggtaaacaat
     1441 cctactttga aaaaaatcca atacaactcc agaagaatcc tcataaagcc tcctcttttt
     1501 attatagcct gttttaggtt aaatattaag agagaaacat gtttcacggg taataagatt
     1561 gttaaattct attaaattat acagcccaca gaggtattat atagtcctgt cgatttatga
     1621 tattctgaca tcggatatta gtttttcata aactgttaca taatttgttt gaaacttgaa
     1681 ttaaccgata ataggaaaaa tatttaccaa acctccgttt ttatatataa ttaaacaaag
     1741 gatggagtat tatgaaattt acaaaaatgc acggagcggg aaacgactac atatatgtgg
     1801 attgtatgga atgtaagatt gacaaccctt cttctttggc gattaaactc agcgacaggc
     1861 ataaagggat aggatccgac ggactggttc tcatacagcc ttcaaagact gcacactttt
     1921 atatgaggat gttcaactcg gacggttcgg aaggtgccat gtgcggaaat gccataagat
     1981 gcgtagcaaa atatgtgtat gacaacaagc ttaccgataa gttaaagttg gacattgaaa
     2041 caaaaagcgg aataaaacac ttaaaactgt ttcccgacaa aaaaggaaaa atagattcgg
     2101 tggaagtgga tatgggtatt gcgtatctga aacgtaaaga aatacctatg gtttatgaca
     2161 actcaaacag ggaggaaaca tatataaaca aacctcttgt cgtagatggg aaaaagtaca
     2221 atgtgacctg tgtatccatg ggaaatcctc actgcgtcat atttatgaat gacaaagata
     2281 ataaaagcaa tcttatgcag tctctggaca aactggattt ggataatata ggtccggatt
     2341 ttgaaaggca ttcgtatttt cccgacagga taaataccga gtttgtggaa atcatagaca
     2401 ggaacacctt aaaaatgaga gtctatgaaa gaggttcggg agagactatg gcctgcggaa
     2461 cgggagcatg tgcaacggtg gttgctgccg tacttaacgg catatgcgaa agaaacagcg
     2521 aagtgtccgt tatattgaga ggcggaacat taaagattat atattcagat gacggcagag
     2581 ttattatgaa aggcgaagcc gtgcatgtgt ttgataccga aattaacata taacattgaa
     2641 aaggagaaga aatatggcaa aaccgaatgt aaactacggc aatcttaaac agagttatct
     2701 tttcagggat atagcaacaa aagttgcggc atataagaag gcgcatccgg acaggaaagt
     2761 tattagcctg ggaataggcg atgtaacatt acctttggca aaaaaagtag tggaagcttt
     2821 gaaggcggca tccgaggaaa tgggtaaagc ggaaactttc agaggatatg ctccagacca
     2881 gggttatgac tttttgcggg aaaagatttc cgaacattac aagaaaagaa atgtaactgt
     2941 tgatataagc gagatattcg tatcggacgg tgcaaaaagc gatttgggta atataacgga
     3001 gattttctcc agagacaata tagtcatgat acccgatccg gtatatccgg tgtatatgga
     3061 taccaacgta atggacggca ggaaaattat atttgccgat gcaaacaaag aaaatcattt
     3121 tcttgccatg ccggacaaaa gacatgccga tatgatatat ctgtgttcac ccaacaatcc
     3181 ctgcggaagc gtatattcca aagcgcagct ggagaaatgg gtgcagtatg ccttgaagca
     3241 taattccgta atactttttg atgcggcata cgaagcattc ataaccgatg ataaattgcc
     3301 gagaagtata tttgagattg aaggagcgga aaagtgcgcc atagagttct gcagtttttc
     3361 caagcttgcg ggatttaccg gtacaagatg tgcttatacg gtaataaaga aagaattaat
     3421 attggaaggt ttttccttac gcgacatgtg ggtaaggaga cagagcacta aacttaacgg
     3481 agtatcctat atcatacaaa gaggtgctga agctgtattt acagaagaag gaatgaagca
     3541 gtgcaaagat aacatcaggt attatctgga taatgcaaaa cttatttccg ctcttcttac
     3601 ggaaaaaggc atatatcaca caggcggaaa caactccccg tatatatgga tgagttgccc
     3661 taataatttg aagtcctggg aattcttcga cttgttgcta aatgaaacat gtgttgtcgg
     3721 aacgcccggc agcggattcg gccacaacgg ggaaggatat ttcagactga catccttcgg
     3781 aagcagagag aacactatag aagcggtgga aagactgaga aaggttcttt agtctgacaa
     3841 ataaactata tagagaaaaa gcaacacaac aaagagcagg cttgcaaaac gttggattgt
     3901 tttgcaagaa agtgctataa tgaaaaacaa tttttatgtt atttttaaaa taagttttat
     3961 ggcatgatga ttatgtttta ggtaagactt tagggaggaa ttattggtgg aagaatttga
     4021 aaaaagatat gacggcttag cagttgaaaa agaaatgcaa cggttttggg aagataacaa
     4081 catatataag tttgtacccg atgtgtctcg cccgctttat tcaattgata cacctcctcc
     4141 gactgtcagc ggaagtttac atatagggca tattttcagt tatacacaag cagagatgat
     4201 tgcacgtttt cgccgtatgc aggggtataa tgttttttac ccgttcggtt ttgatgataa
     4261 cgggcttcct tcagaacgtt tggtggaaaa ggaaacggga ataaaagccc atgatatacc
     4321 aagaagtcag tttcagaaaa gttgcataga aataacgaaa aaatatgaag acgagtttat
     4381 gaatctgtgg aaatccatgg ggttttcttg cgattggaat ttgcaataca ctacaataag
     4441 ttccaataca cagcgtttgt ctcagaagtc attcttggag cttgcaagag caggtcatgc
     4501 atacattaaa gaatcaccgg ttctttggtg tactgaatgc caaacctcaa tagcacaagc
     4561 agaactggat tcaaaagaaa ttgaaacata ttttcattat ataccttttg gtgaaggaaa
     4621 aataacggtg gaaattgcca ccacacgccc cgaactgctt tacggtgtag tttgtgtatt
     4681 tgttaatcct gaggataacc gttactcgga tttgatcgga aaaactatca aggttccgct
     4741 atacggtttt gaggtgccta tattggcgga tgaaaaagtc gctattgata aaggaacggg
     4801 agcggttatg tgtgctactt tcggagacac aaccgatgtt gaatgggtta ctcagaaggg
     4861 cttagaatac aagaaagtta ttctttctga cggaacaatt gcttcagatg ttccgtttat
     4921 agcggggatg aaggttaaag aagctcgtag aaagatagtg gagttattag aagaaaagag
     4981 agtattgtta aaatctgaaa agcttactca tattgtttca gttcatgagc gctgcggtac
     5041 tgaagtggag ataattcctt caagacagtg gtatattgat gttttaagca aaaaagaaga
     5101 gttgcttgta gccggggata gagtaaactg gtatcctgaa catatgcaga acagatatat
     5161 tgactgggta gagaacttga aatgggattg gtgcatttcc cgccaacgct atttcggtat
     5221 acccattccg gtttggtatt gtaaaaactg cggaaaacct gtttttgcag agtacgaaca
     5281 attacctgta aatccgatgg aaacaaatta ttccggcaaa ggctgtgatt gcggttgcaa
     5341 cgagtattta ccggacaaag cggttttcga tacatgggcc acatcttcca taacaccgca
     5401 actgaattgc gataaagctg ccgaattcgg gattaatgag ggttttatac cgatgagcat
     5461 gagaacgcag gctcatgaaa taattcgcac atgggctttc tacactattg taaaaagctt
     5521 atatcacacc gatgatatac cttggtcgga tattatgatt tgcggctttg ttcttgccaa
     5581 acccggtgaa aagataagta aatcaaaaga caattctaaa ctatccccac gagaattgat
     5641 taatcaatat tctgctgatg ctatcaggta ttgggcggca aatgccagat tgggcattga
     5701 tacatacttc gatatacagg agatgcgcga tgcatctaat cgtttaatga ctaagctatg
     5761 gaactccgca aaattcgttc tttcacattt gaccgattat aatccgaaat atgtacccga
     5821 gtatttattg ccgattgaca aatggattat tgagagaaca aatgaagcta tcttagatgt
     5881 tacaaaatgg cttaatgagt atgagatagg tcttgctcgt aagattgtag atgatttgtt
     5941 ttggaaagac ctttgcgatt attatattga aattgtcaaa gagcgtttat accgaccgga
     6001 tatacatggt gttaaagaaa gaaagtccgc gcagcatgca atttactatt gtttgttgaa
     6061 tgttttgaaa atgtattcca tctatattcc tcacgaaacg gagtacatct atttaaaagg
     6121 atttaaagat tttgtcggaa taaaatctat tcatctcacc caatgggaga agccggtgtc
     6181 tgtagacaag tccattattg aatttggcga aacggtaaag gattcaattg cggaagtcag
     6241 aaagttcaaa tcggaaaaca atttatccat gcgttccgaa attgatgaag ctgtcataaa
     6301 ctacaggaga gcacattttg atcggttaaa agaaaccgaa aaagacttca tagcctgtac
     6361 tcatgtcaag aaagtcagct ataaattgat ttaattgatt ataacaaagc acagatatag
     6421 gaagaataca agaatttttg gtaatagaat gcattatttt ttggtaacgg aatggttgat
     6481 tttttggcaa tagggcatta taatgtagtt acgaggtgct ctgatgaaca ttataaatta
     6541 cagaaaaaga ctgattgaat ctcaaattga gaaaaaaatg tcctatagcg gcggcgtcat
     6601 tattgaagga gcaaaatggg tagggaaatc tacaacagcc ggtatttttt ccaatactgt
     6661 tataaaactg caagacccgt tggttaaaaa acaatatcaa attttggcta ccatatcaaa
     6721 agatgaggtt ttaaagggtg aaaaacctat attgtttgat gaatggcagg aagttccgga
     6781 aatttgggac tttattcgtc ttgatattga tgaaaataat tataaaggtg cctaccttct
     6841 gacgggatcc accaaaaaac aaaacataac aacttcacat acgggaacgg gaaggattaa
     6901 ctcggtttac atgcgtccta tgagtttata tgaaaccgga gaatctaacg gactgatttc
     6961 tttaaaagaa ttgttttcac aaccgccatt agtcccggtt aagtcaaata ttacaattga
     7021 taatatagcg cactgtattt gcagaggcgg ttggcccgga agcctggact taaacaaaga
     7081 cttacaattg gaaattccta aagatttact tgaaagtatt atcaaaagag acatagacga
     7141 agttgacgga attactaagg acaaagagaa atttagaaaa attattaaaa gttatgcaag
     7201 gaatatttct acattagcag ccaattccac tatttatgag gatcaatctt atgaagttat
     7261 tgtaaatccc aaaacatttg atacatatat gaactcctta aaaaggctat atataataga
     7321 agatgttttg ccttggtcgc agaatataag aagttcaaca agactgagaa aaggaaccaa
     7381 aagacaattc gtagacccca gcattgcagt tgccgcttta ggtttgtctc ctgataaact
     7441 gaaaaacgat tttgaaacat tcggtttttt atttgaatcc attgctacaa gagatatcag
     7501 agtttatgcg gaagaaatcg gaggaaccgt atattactac aacgattcaa caggacttga
     7561 agtggatttg attgttgaat tgaatgacgg aagatgggga ggaatagaag taaaattagg
     7621 agaaaatgaa gctgataaag caagtgctaa tttaataaaa ttagcaaata tatccgctac
     7681 taaaccatcg ttccttatgc tgttaacaaa cactcaaatg gcctatcaaa gagatgacgg
     7741 tgtatatgta ataccgcttg gatgtttaag gccatgatac tattctaatg cgtatttgtt
     7801 gaaatttcac atttttgtaa tgcgtatttg caggaaaagg cttgttttat aatgcgtatc
     7861 tgtagtatta tgtgtttagc taacggcgtt attgttgaaa aaagaggaaa atcatgttta
     7921 aaagaaagat atacgataaa ttattggagt ggaaagaaac atcgaaaggc agaacggctt
     7981 tactgataga gggagcaaga cgtgtaggga agtctaccat tgtcacggaa ttcggaaaga
     8041 atgagtacaa atcatatata ctcatagatt ttgcctttgc tccgaccgga gtaaaagaat
     8101 tatttaacga tatcagtgat ttggattatc tgtttttgca actgcaactg caattcaaag
     8161 cggacctgca tgaaagaaat tcattgataa tatttgatga agttcagttt tgtcccaaag
     8221 ccagacaagc tgtaaagaaa cttgtagaag acggcaggta tgactatata gaaacaggtt
     8281 ctttaatatc aataaagaaa aatgtggagc acattcttat accgagtgag gaaagaaaaa
     8341 tagaaatgtt tccgatggat tttgaggagt ttttatgggc aatcggagac aataccacaa
     8401 acaacatttt gagaaaattt tataatgaga aaatttcacc gggtcaagat caaaacagag
     8461 ctttaatgag aaagttcaga ttgtatatgt tagtcggagg tatgccacaa gctgttaaag
     8521 catatataga gacaaacaat ttcaaatttg tagatgaggt taagagagat attattcagt
     8581 tatatgaaga agacttttat aaaattgatt caagcgggaa aatatccatg ctttttgatt
     8641 caataccctc ggaattaagc agacactctt ccagatacca ggtttccagt gtgttaaaga
     8701 atcaaagagc cgctactata tccgaggaaa ttgccgaact ggttgcctct aaaacggttc
     8761 tcatttctta ccactcaaac gacccgtccg taggaatgac aacgaattta agtatggagc
     8821 atttcaggtt atttatggcg gataccggct taatggtaac atgcatgttc aaagataagg
     8881 atttcacgga caatataatc tatgagaagt tgttgaacaa caagttgtca aaaaatctgg
     8941 gggtacttta tgaaaacatt gttgctcaaa ctttggcttt caacggaaac aagctgtttt
     9001 attatacttt ttatgacgaa actcagaaaa gaagttttga ggtggatttc ctgctttcca
     9061 aagataataa ggtaagtccg attgaagtaa agtcatcaaa ttacagaaca cacagatcta
     9121 tggatgagtt tgcaaataaa tactcaaaca gaataaacac caaatatgtt attcacactc
     9181 aagatttaaa aaagaacggc aatttaattt atttaccgtt ctatctggca cagtttatat
     9241 aagaatagtt gcttatttta tcaatccttc ttccagttgt ctgcttttta ccttttccat
     9301 cagcttgtct attctgtcta ccggattgag aaggttggac atggaagcgt catgatgtaa
     9361 ggtatctatt atgaaagaca tgtatttgga catatccact tccttgaacc agggtctctt
     9421 aagtaattcg ggacttctgt atacaagatt ggtggtaagt actccggtaa ccgcaccttc
     9481 ctcataagct ttgtcaaaag cttcgagacc gttgcagaac aaaccgaaag tgcaacacat
     9541 aaatattctt tttgctttca tttttgtaag ctgatagaag atgtccaatg cagattcgcc
     9601 cgatgagata atgtcttcaa caactattac atctttgcct tcaacactgt ctccgaggaa
     9661 ctcatgcgat attatggggt ttctgccttg ctccacaacg gaaaaatctc ttcttttata
     9721 gaacataccg agatttaagc caatcatggt tgcaaaggga attgctctgt tcattccgcc
     9781 ttcatcgggg gatattacca aggtgtgctc tttatcaaat atgatatcgg ggaaagatgc
     9841 agtaagcttt ttcatcatct gatatgcaac cggaaccgat tcaaaaccgt tcagagggat
     9901 tgcattttga attctgggat cgtgagcatc aaacgtaatt atattctcca cccccatgcc
     9961 tacaagctcc tgcagagaaa tagcggaatc caacgattcg cggtattttc tcttgtgttg
    10021 tctgccttca taaagcatag gcattatgac ggtaattctt cttgccttac cgttggttgc
    10081 ggctataatt ctttttaaat cggcataatg atcatcgggg ctcataggca cctgcatccc
    10141 gtacatttca taggttacac cgtagttgaa aacatcgcag attatgaaga ggtcgtgtcc
    10201 tcttatagat tccagtatga cacctttagc ttctcccgtt ccgaatctag ggcatatcgc
    10261 ttcggttata aatgtatggt cggaatcctt gtagccggct ctttgtttca gataccaatc
    10321 aacttttttt gttatttctt cacaaccgcg cattccgatt attgataatt ttcctacggg
    10381 aagcacggtt tcaaaaggtt tgatcatatc aacaactccc ttcgttaata cactgtcaat
    10441 ggtatcattt gtccaggtat ttatcaatag cggcggcggc tttcttgccc gctcccatgg
    10501 ccagtattac ggttgccgca ccggtcacgg catcccctcc ggcgaatata tacggaatgg
    10561 aagtttgtcc ggtttcctca tccgcaacaa tgcaacccca cttgtttgtt tccagacctt
    10621 cggtagtgct ctttatcaaa ggattggggc tggtgcctat tgccattatc accgaagtgg
    10681 tttttaaaac atattcggaa ccgggtataa ccacaggtcg ccttcttccg tcaggaccgg
    10741 gttcggaaag ttgcatcctg acgcattcca tacctgttac ccatccggtt tctcccaata
    10801 tttttgtggg atttgtaagc agacggaatt ctataccttc ttcttttgcg tgatgtactt
    10861 cctcggctct tgcaggcatt tcctgctcgc ttcttctgta tacgatatat acctgttccg
    10921 ctcccaacct ttttgcggct cttgcggcat ccattgccac attacctccg ccgacaaccg
    10981 caacatgact gccgaaatat atgggggtat cgtaagaagg atcatatgcc ttcataagat
    11041 tgacacgtgt taaaaactca tttgccgaaa aaacaccgtt caggttttca cccggtatat
    11101 tcataaaatt gggcaatccc gctcccgtgc ctacaaagat tgcttcaaaa ccttcttcct
    11161 cgtgcagctc ctgtatggag attgtctttc ctattaccat attcaactct atacatacgc
    11221 cttttgcctt gagtcgttcg atttcctttt ccactatgga ttcgggcaga cggaattccg
    11281 gtatgccgta gactagtaca cctccggctc tgtgtaaagc ttcaaatatc gtcacatcat
    11341 agcccatatc gcataagtcc gatgcggctg taaggcctgc aggaccgctt cctattaccg
    11401 cgactttttt gttcttcttc accgtaggtt taggtgtatc ggctataaag gtttcttttg
    11461 caagcatata gtctgcaaca tatcgctcca gtcgccctat agcaaccggt tctcctttta
    11521 tgcctctcac gcagggcagt tcgcattgtg tttcctgagg gcatactctg ccgcagatag
    11581 cgggaagcga attggttttg gtaataatct cataagcttt ttcaatatcg ccctgagcca
    11641 ccgactgtat gaatgcggga atgtctacat ttaccgggca tccggaaaca cagggtttgt
    11701 gtttgcaatt gagacagcgc attgcttctt cctgagccat ttcgggagta tatcccgtcg
    11761 caacttccag aaaattgcga tttcgtatat cgggattctg ctccggcatt tttgttttct
    11821 ccggagtcat atttattttt gccattattc gatacctcta aacagattac atttgtgctt
    11881 ttcctgttct ttatacatgg aaagtctttg cataattcca tcgaaatcta ctttgtgtcc
    11941 gtcaaaatcc ggtccgtcaa cacatgcgaa cttggtctga ccgtcaacgg tcagtctgca
    12001 tcctccgcac attccggtgc cgtctatcat aatgggattc atgctcacta tggttttaat
    12061 gttgtgttct ttggtaagta aggatacata cttcatcata acaacaggac ctattgctat
    12121 taccgtatca tagtttgctc cgccctctat aagttccttg agctttttgg taaccagacc
    12181 tttttctccc gccgacccgt catcggtcat gagataatag tttgtgctgt tatgtttcat
    12241 ctcttctttc agaaatacca acgacttgtt tcgaaaacct gctattatgt cgacatgaag
    12301 gcccataccg aagagttttt tggcttgcgg gtaagctatt gcacaaccta agcctccgcc
    12361 cactacacaa actctgctca tattgtcggt gtgtgatgca attcccagag gacctacaaa
    12421 atccagtata taatctcctt gctgaagggt gtccagaagg taggtggttt ttccgacttt
    12481 ttgaaagatt atgcttaccc agccttcttc acgattgtag tcggatatcg tcagggggat
    12541 tcgttct
//
LOCUS       DAOPWC010000003        12508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_5138_length_12508_cov_4.052036, whole genome shotgun sequence.
ACCESSION   DAOPWC010000003 DAOPWC010000000
VERSION     DAOPWC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5138_length_12508_cov_4.052036"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKJ65_00140"
     CDS             complement(<1..532)
                     /locus_tag="PKJ65_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40275.1"
                     /translation="MKRAILSLITIILLLSMCINAFAYCESFERTGKHINVAYVSDVH
                     TPQGHQCGYICQDCELITVTGYTTLSTCCRCTGNHNYYTLSVGSHTAYGHLVKLKCST
                     CGYTTSYYKKDDSCCDCVGHIRGLMEINTEHTQGLGHYYTVPCKECGAIIESGYTTLA
                     SCAICNCSHSYTTSISD"
     gene            complement(583..1755)
                     /locus_tag="PKJ65_00145"
     CDS             complement(583..1755)
                     /locus_tag="PKJ65_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40276.1"
                     /translation="MKKFTSLLLVICLIPAAGVCGVNAQAAGSRFSDVQSSQWFFEPV
                     IQLYGMNIINGYPDNTFKPDGNVKADELIKMIIKALGFDIGNAEGYWAQNYIDKAGDI
                     KLLEGFDGEYQKEINRGQAALLISNAMTYLKEPEFYSEKIYPFDLGAYTPDKYKEHIK
                     KCYNAGVINGFEDFTFRFNETLTRAQACTLLIKLIDKTKRDTSVRFDCSFLDNTTKDN
                     VQYKGVTYEELSDVIKSTLTEVKDDIGYIVVEKRVYETKNTYEIRYHKSAINTNPTYC
                     LFAFRFFEGPSGYTETQWGYDTMFLKLELKKFNWDDGIPDSFYKSKVKNALCAALSEH
                     NPYDIADYVIDRYVFLRALKPWDAYNLLEDNSNETLRYVFFTMHNVIGNFTFSNKK"
     gene            complement(1908..2477)
                     /locus_tag="PKJ65_00150"
     CDS             complement(1908..2477)
                     /locus_tag="PKJ65_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ40277.1"
                     /translation="MKPDDFLQWLHVIEKMKCNFRHSKTSCGREESVAEHSYRLILMA
                     YVLKDTIPDVDMDRVVEMCIVHDIGEAITGDIPSFRKTKEDDKKEMEQVLEFIRQLPE
                     ERYQHIKELFSEIEQKKTKEAIVFKALDKMEAVIQHNEADISTWLDIEYELQKTYGTE
                     EAQVCEFLKTLRNQVLKDTEEKIEKSKRE"
     gene            complement(2477..3259)
                     /locus_tag="PKJ65_00155"
     CDS             complement(2477..3259)
                     /locus_tag="PKJ65_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40278.1"
                     /translation="MKNKIFKTLTLVLLISLLCTTLTGCKIQLSEDEYTVGEIPKGCY
                     RLVYYIVNEDQNIDGDLTFKYVTDGNITKMQSYAEFDMWQYRAQVIFDTDTLIPSSSY
                     KSNVFYSGKEKDWSVRTDYTKEKATIKAIQEDKQADKEVAKPARYLDNEMINLTLGLI
                     EIEQGDKVYINVLVNESAMFAPYGVTNQTMETITVNGKSYECVKYSAKYAGFSLFTAR
                     ESYFWYTNDRDRRLVKFVSGSQIMTLKEIIEVPADDPWLFGE"
     gene            complement(3261..3527)
                     /locus_tag="PKJ65_00160"
     CDS             complement(3261..3527)
                     /locus_tag="PKJ65_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40279.1"
                     /translation="MLIYGKLIKNNLILVEEKAECVNPDMNFQQRLTNCLVDICKKMD
                     IQVPVWMSKNTKELARFNKTFFTDEQFMEKVYFDRFEIEMKRKD"
     gene            complement(3646..4611)
                     /locus_tag="PKJ65_00165"
     CDS             complement(3646..4611)
                     /locus_tag="PKJ65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ40280.1"
                     /translation="MKNTIDITEKVMINGIQQKIHIIGTDSVNPVVLFIHGGPGVVDR
                     HNVMKHMLDITDKVTIAGWDQRGTGGSYKGAKKEDMNLDTFVEDAREITEYLCKKFNK
                     DKIYIQGHSWGSCVGTMLVYRYPENIKAYFGQGQLVNGIRNEEKSYDYVLEKAKAAGN
                     EKDVQKLLEIKRPVEGLYAGDPIKGLMEQRKLLSKYGGATFKCSSMWKSTVKPILFSK
                     EYTLCDIAGVLKGYSFSLKNMWEDIVKLDFSQEYTKFSVPMFYLIGASDYNTPFELSY
                     EYYKTVEAPLKDYIWFNRSSHSPLAEEPEKFSRTLAEKILQVEAM"
     gene            complement(4595..6334)
                     /locus_tag="PKJ65_00170"
     CDS             complement(4595..6334)
                     /locus_tag="PKJ65_00170"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ40281.1"
                     /translation="MEDFRYIDRGKPIPREQYTDQPYVVVADDGAWVCVLTTAYGQEG
                     AAGTHVGITVSYDRGNTWTDLYFPEGNDLRESAYGVLLKTSFNRIYCFYNFNKNNLRN
                     LICRKERLSHRVDMQGAFVFRYSDDSGKTWSERYEVPIRETKIDRENPYGGKVRMFWT
                     VGKPFILDNDGYVIIHKIGDMLTVSEGWLLRAVNMNTEKDPNKLVWETLPDGDIGLIT
                     PKGGGLIAEEQSMVVLSDKSIYCVYRSIDGHPVETYSRDKGHTWEQPQYKRYADNRLM
                     KHPRAANFVWKCKNGKYLYWYHNNGSKWYENRNPAFLCSGIEYDTKEGRRIKWSQPEV
                     LLYHEDPGVRMSYPDFIEDSDKYYITETQKLDARVHEIPEKFLQSLWNQFESSDITDE
                     GLLSESEGKSVIDMPELPVFCITDRKSHIFATKSTESGVTFELIYKAGTGKRKILDSR
                     DASGKGVLIEQICDCRINILMNDGVNTCYRSSDKYMLKPDQINHIAIIIDGGPNIVSY
                     VINGMYNDGGQDRIFGFGRFSTYFKDMNGKTKACVCKNVLKLRIYGRALLTSEIISNG
                     RGYKKGGKYEKYD"
     gene            complement(6392..7072)
                     /locus_tag="PKJ65_00175"
     CDS             complement(6392..7072)
                     /locus_tag="PKJ65_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNZ40282.1"
                     /translation="MRSKRKKKKKNRLQKAIAVILLISALLFTSLQLYVMLYPLSDIF
                     DSVEQLGKIERPQAVLVLGAAVYASGNPSPVLKDRLDYAYQVYEYYDGKIAVIVSGDN
                     GTVEYNETKAMYDYLISKGVNADDIYRDYAGFNTYDSIYRAGYIFKVQSIIICTQRFH
                     IYRALYIAKRLGFKAYGYAAENKSIYNMTYNHIRESLARIKAVMETDIMKRQPKYLGD
                     PIPVKQYE"
     gene            complement(7062..7931)
                     /locus_tag="PKJ65_00180"
     CDS             complement(7062..7931)
                     /locus_tag="PKJ65_00180"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ40283.1"
                     /translation="MKKCDILVPIFTPFKKDQTVDYEALKKLVRYVLDKGADGLYTTG
                     SSAETFLLTEEERMKTLEVAAKEANGATVVAHVGTPGTALAVMYAKHAKSMGVDAVAS
                     VPPFYYSHGHKAIKKYYTDIADGCGLKIMIYNIPGATGVDMSVDQINDLLSDDRIYSI
                     KYTAADYYVLNRIAVASKKPIYSGKDEAFASALMAGATGAIGTTMNIYPQAFVQIKAA
                     FDKKDIASAQKLQTKLNNIIQPMIESGATIQAMKYVSKLAGIDCGEARRPFKNLTAED
                     KKKLEQAVRDNAF"
     gene            8051..9160
                     /locus_tag="PKJ65_00185"
     CDS             8051..9160
                     /locus_tag="PKJ65_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ40284.1"
                     /translation="MKDPRNEKLADLLINYSCRLKEGEKILISVSEIDNDLVKRLVEK
                     AYQAKALPFVQISDSSLSPKLYEHITEEQLEIMNERDLNFMKKMDAYIAIRASKNIYE
                     ISTVPSEKLSLASRILKPSFDRRVKYTKWCVLRYPNESMAQLAGMNTEKFTDFYYDVC
                     TLDYKKMSDAMDNLVELMNKTDKVHIKGEGTDLTFSIKDIPAIKCAGEMNIPDGEVFT
                     APVKDSVNGYVTFNTPSVSEGKTFENIRLEFEKGKIVKATANYTEHLNKILDTDEGAR
                     YVGEFAIGVNPYILDPMKDTLFDEKICGSFHFTPGACYDEAPNGNDSAIHWDLVCIQT
                     EQYGGGEIMFDDRLIRKNGIFTVEELHCLNPENLK"
     gene            9168..11369
                     /gene="csaB"
                     /locus_tag="PKJ65_00190"
     CDS             9168..11369
                     /gene="csaB"
                     /locus_tag="PKJ65_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNZ40285.1"
                     /translation="MKIMHLAGGGDIGGAKTHIISLAKELSRTNTLKLVSFRYGDFAE
                     SAKAEGIDVTEIKSAFLLKDIHALFIEYDKFKPDIVHCHGAKANMMGLFLKRHRNACV
                     TTTIHSDYRKDYMHNLFKQATIGLFNTLALRIIKFHIAVTEQFARTLISRGFEAYNMF
                     SIYNGIDFEKEMPKISRQEYFEQYGIPYNPDELIIGIAARLTKVKDIPTLIKGFAKAH
                     ADNSDMRLLIGGDGEEKQKLTDLANQLGVSEKVHFLGWIDDVDSFFSVIDIDVISSLS
                     ETFPYSLTEGIRAGAIPVSSNVGGASSLIDQKVTGYIFEPKDYETLGNYLYQLSKDKN
                     KRITMQKASYEHTKANFSLKAMKNKQEDIYSLILKHFNEKERNKVVICGAYGRGNAGD
                     EAIKYTTLSSMRNIDPDMDICVMTLRPKKERAIDRVQTIYTFNVIKLFFLLRKSKIFV
                     SGGGSLIQDVTSSRSLFFYTFTLYLAKKLGCKVIMFGCGIGPVSRPFNRKLSGKIIDN
                     YVDVITVRDHMSEKELQSLGVTKPQITVSADPAINLTADKSNTHKIFISEDIPADGKY
                     ICLALRSWKNFNRNEAVAKACEHMYNKYGFTTIFFPMEYPKDLSMSEKTAELLDTPYY
                     LIRNKYPTEQVVAFMSKMEQVVAMRLHALIFAAANYVPVVGISYDVKVSGFMNYIESS
                     ACLELSDVTEHSVINAMEESFKHRERVIKATNTLKEKEAVNKQQFIKLLEN"
     gene            complement(11743..>12508)
                     /locus_tag="PKJ65_00195"
     CDS             complement(11743..>12508)
                     /locus_tag="PKJ65_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ40286.1"
                     /translation="TYGNLPGWVQIWNPPYEDADMLLIPTHADDEHLYFGGMMPYYAG
                     QLQYKVQIAYLTNHWGEPYRPHELLNGLWTVGMTAYPVISEFEDKYCSNLEHAKSVYP
                     MEDVLNFQVELLRRFKPEVVIGHDLKGEYGHGAHMLNAYALTLAVEYAADSTKYVSSY
                     EKYGVWDTPKLYLHLYEENKILMNWDIPLERFNGLTAFEMAVQGYDCHKSQHIWSFAV
                     RQGESQYDCRWFGLYRSLVGPDIEKNDVFENILSGK"
BASE COUNT         3473 a         2756 c         2278 g         4001 t
ORIGIN      
        1 aatcggatat gcttgttgta taggaatgac tgcaattgca gatagcacat gacgccagtg
       61 ttgtatatcc gctttctata atcgctccgc attccttaca gggaacggtg taataatggc
      121 ccagtccctg cgtatgctcc gtgtttattt ccatgagacc tcgaatatga ccgacacaat
      181 cacagcagct gtcatctttt ttatagtagg aggtcgtgta tccgcaggtt gagcacttca
      241 attttaccaa atgcccgtaa gcggtatgag atccgacaga aagtgtatag tagttatgat
      301 taccggtgca ccgacaacat gttgaaagtg ttgtatatcc cgtcacggta atcaactcgc
      361 aatcctgaca aatatatccg cattgatgac cttgcggagt gtgtacatcg gaaacatatg
      421 cgacatttat gtgttttccg gttctctcaa aactttcaca atatgcaaat gcgttaatgc
      481 acatgcttaa cagtaaaatt atcgtaatca aagacaggat agctcttttc atgttatctt
      541 ctccataagt tgcgctattt attttgctct atacttacct tttcattttt tatttgaaaa
      601 agtaaaattg cctattacat tgtgcatagt gaaaaagaca tacctcaatg tttcattgga
      661 attgtcttcc aacaggttat atgcatccca aggttttaaa gcacgtaaaa atacatatct
      721 gtctattaca taatccgcta tatcataagg attatgttcg gataaagctg cacacagtgc
      781 atttttaact ttactcttat agaagctatc gggtataccg tcatcccagt taaacttctt
      841 cagttccaac tttaaaaaca ttgtatcgta accccactgt gtttcggtat atcccgacgg
      901 accttcaaaa aacctgaaag caaacaaaca atatgtgggg ttggtattta tggcggattt
      961 gtggtatcgg atctcatatg tattcttggt ctcataaact cttttttcca ccactatata
     1021 cccgatatca tcctttactt ctgttagagt ggattttata acatcggata attcctcata
     1081 tgtaacaccc ttgtattgca cattatcttt ggttgtatta tccaaaaacg agcaatcgaa
     1141 tctcaccgag gtatctctct ttgttttatc gataagcttg attaataagg tgcaggcctg
     1201 tgctctggta agcgtctcat tgaatcggaa agtgaaatct tcaaacccgt taataactcc
     1261 cgcattgtag cactttttta tatgctcttt atacttatca ggtgtatatg cgcccaaatc
     1321 aaacggatat atcttttccg aataaaattc cggctcctta aggtatgtca tggcattgga
     1381 gataagcaag gctgcctgcc ctctgttaat ctctttttga tattcaccgt caaatccttc
     1441 caaaagctta atatcacctg ctttatctat gtaattttga gcccagtaac cttcagcatt
     1501 acctatatca aaacccagcg cttttattat catttttatc aactcatcgg ctttgacatt
     1561 gccgtcgggt ttgaatgtgt tatcgggata tccgtttata atattcattc cgtacaactg
     1621 tatgaccggt tcaaaaaacc actgggatga ttgcacatcg ctaaaccgtg aacctgcagc
     1681 ctgagcatta acaccgcata ctccggctgc cggtattaag catatgacaa gcaataaaga
     1741 tgtaaatttc ttcataatac tcctttcgag tatcatgcta ttgttattgc caatatatgt
     1801 ctactgacaa aaatgctttt ttttatacta taagaaaata ttccgacaat ttttggaacc
     1861 cgattccgtt aatcacgaaa tattgcaaaa agtcaatatg taaaaaacta ctctctttta
     1921 cttttctcta tcttttcttc ggtatctttc agtacctgat ttcttagggt ctttaagaat
     1981 tcacaaacct gtgcttcttc tgtgccgtag gttttctgca gctcatactc tatatccaac
     2041 caggtagata tgtccgcctc attatgctgt ataaccgctt ccattttatc caatgcttta
     2101 aagactatcg cctcttttgt cttcttttgc tctatctcgg aaaagagttc tttgatatgc
     2161 tgatagcgtt cttccggaag ctgccgtata aattccagca cctgctccat ttctttttta
     2221 tcatcttctt ttgttttcct gaaagaaggt atatcccccg ttatcgcttc gcctatgtca
     2281 tgaacaatac acatttccac aactctgtcc atgtccacat cgggtatagt atcttttaat
     2341 acatatgcca taagtatgag ccggtaggag tgttccgcaa ccgattcctc tcttccgcag
     2401 gatgtctttg aatgccgaaa attgcatttc atcttctcta taacatgcag ccattgtaaa
     2461 aaatcatcag gtttcattat tcaccaaata accatgggtc atcagccggc acctctataa
     2521 tttcttttaa agtcattatc tgagagccgc ttacaaattt caccaatctt ctgtctcggt
     2581 cgtttgtata ccaaaaatag ctttctcttg ccgtgaataa ggaaaatccc gcatacttgg
     2641 cagagtactt gacacactca taagacttac cgttaacggt aattgtttcc atcgtctgat
     2701 tggttactcc ataaggagca aacattgcac tttcgtttac aagcacattt atgtacacct
     2761 tatcgccttg ctcaatctca attaacccga gtgtcaggtt aatcatttca ttgtcaagat
     2821 atcgtgcagg ctttgctact tccttatccg cttgcttgtc ttcctgaatc gcttttatag
     2881 ttgccttttc tttcgtatag tcggttctta ccgaccagtc tttttctttt ccggaataga
     2941 aaacattgga tttgtacgaa gaagacggaa taagagtatc ggtatcgaat atgacctgtg
     3001 ctctgtattg ccacatgtca aactccgcat atgactgcat ttttgttatg ttcccgtctg
     3061 ttacatactt gaatgtgagg tctccgtcaa tattctgatc ttcgtttact atgtagtata
     3121 ccaggcggta gcaccctttc ggtatttcac cgaccgtata ttcatcttcg ctaagctgta
     3181 ttttgcatcc ggttaaggtc gtacaaagca acgatatcaa taaaacaaga gttaatgttt
     3241 taaatatttt attcttcatc ttaatccttc ctcttcatct ctatttcaaa tcggtcaaag
     3301 tatactttct ccataaactg ctcatcggtg aaaaatgtct tgttgaatcg tgcaagctct
     3361 ttggtgtttt tactcatcca taccggaacc tgtatatcca tcttcttgca tatgtccaca
     3421 agacagtttg tgagtctttg ttgaaagttc atgtcgggat tgacacattc ggctttttct
     3481 tcaaccagta ttaaattgtt cttgataagt ttgccgtata ttagcattat tttccgtcct
     3541 gctcattttg tattgcctat tttatcactg aatgagtgtt ttttaaacat tttatcttat
     3601 cgcctattgc tatattgtgc atgtaaagca agttcctgcc ctgacctaca tcgcttccac
     3661 ctgcagtatt ttttccgcaa gagttctact gaacttctcc ggttcttccg ctaacggact
     3721 gtggcttgat cggttaaacc atatatagtc tttaagcgga gcctctacgg ttttatagta
     3781 ttcatacgac agttcaaacg gtgtattgta atcggaagct cctatcagat aaaacatggg
     3841 aacggaaaac tttgtatact cttgggaaaa gtccagctta actatatctt cccacatgtt
     3901 tttcagagaa aaactgtagc cttttaaaac accggctatg tcacacaatg tgtactcctt
     3961 tgagaaaagt atcggtttaa cggtactctt ccacattgat gagcatttga atgttgcacc
     4021 gccgtatttt gataaaagtt ttctctgttc catcaatccc tttatagggt cgcctgcata
     4081 aagcccctct accggtcttt ttatttccaa aagcttctgc acatctttct cattaccggc
     4141 agcctttgct ttttccagca cgtagtcata acttttctct tcatttctta tcccgttcac
     4201 aagctgcccc tgtccgaaat acgcttttat attctcagga tatctgtata ccagcattgt
     4261 gcctacgcag gatccccatg aatgtccctg tatgtatatc ttgtctttat tgaatttttt
     4321 acaaagatat tccgtaatct cccttgcgtc ctccacaaag gtatccaaat tcatatcctc
     4381 tttctttgcc cctttatagc ttccgccggt acctctttgg tcccatcccg ctattgtcac
     4441 tttgtcagtt atatctaaca tatgcttcat tacgttatgt ctgtccacta caccgggtcc
     4501 tccgtggata aagagtacaa cgggattaac gctgtctgtt cctataatat gaatcttctg
     4561 ttgtatcccg ttaatcatta ccttttccgt tatatcaatc gtatttttca tatttacccc
     4621 cttttttgta gcctcttccg ttggatataa tttcggaagt caaaagtgct ctgccatata
     4681 ttcttaactt caaaacattc ttacacacac atgcttttgt ttttccgttc atatccttga
     4741 agtatgtgct gaaccgaccg aaaccgaata ttctgtcctg acctccgtca ttgtacattc
     4801 cgttgatgac ataggaaact atattgggac ctccgtcaat tattatggct atatggttta
     4861 tctgatcggg tttcagcata tatttatcgg aactccggta acaggtattt acaccgtcat
     4921 tcattaatat attaattctg cagtcgcata tctgttctat cagcactcct tttcccgaag
     4981 catccctgct gtctaatatc tttctctttc ccgttcccgc tttgtatata agctcgaagg
     5041 taactccgct ttcggtactc tttgttgcaa atatgtgact cttcctgtcg gtgatacaga
     5101 atacgggaag ttccggcata tcaattaccg atttgccttc gctttctgaa agtaacccct
     5161 catcggttat atccgatgac tcaaactgat tccaaaggct ttgcaggaat ttttccggta
     5221 tttcatgcac tcttgcatcg agtttttgtg tttcggtaat gtagtatttg tcgctatcct
     5281 ctatgaaatc cggataactc attctgacac cgggatcttc atgatataaa agcacttccg
     5341 gctgcgacca ctttatcctc ctgccttctt ttgtgtcata ttcgataccg gaacataaaa
     5401 atgccggatt tctgttttca taccatttgc ttccgttgtt gtggtaccag tacaggtatt
     5461 tgccgttttt acacttccat acgaagtttg ccgctctcgg atgtttcatc aatctattgt
     5521 cggcatacct tttgtattgc ggttgctccc atgtatgtcc tttatcgcga ctgtatgttt
     5581 ccacaggatg accgtctata gaacggtata cacagtaaat acttttatcg cttaatacaa
     5641 ccatggattg ttcttcggca atcaatccgc cgcccttagg ggtaataagt cctatatcgc
     5701 cgtcgggcag tgtttcccac acaagcttat tgggatcttt ctcagtgttc atattaaccg
     5761 ctcttaacag ccagccttcg gatactgtaa gcatatcgcc tatcttgtgg attataacat
     5821 agccgtcatt gtccagtata aaaggtttgc ccacggtcca gaacatgcga acttttcctc
     5881 cgtacggatt ttctctgtct atctttgttt ctcttatagg tacttcgtat ctttccgacc
     5941 aggtctttcc cgagtcatcg gagtatctga atacgaaagc tccctgcata tccactctgt
     6001 gagacagacg ctcttttctg catatcagat ttcttaagtt gtttttattg aaattataga
     6061 aacagtaaat cctgttaaac gatgttttca gcaacactcc gtaagccgac tctcttaaat
     6121 cattgccttc cggaaagtac agatcggtcc aggtattgcc ccggtcataa cttacggtta
     6181 taccaacatg tgttccggcc gctccttcct gtccgtatgc ggttgtcaga acgcagaccc
     6241 atgctccgtc atccgcaacc acgacatacg gctggtcggt atactgctcc ctgggtatag
     6301 gttttcctct gtcaatgtat ctgaaatctt ccattttgcc acctctttaa aacacggtgc
     6361 atatacactg cttatataac tgcataaatc actactcgta ctgttttacc ggtatggggt
     6421 caccgagata tttgggctgt cttttcataa tatccgtttc cataacggcc tttatcctgg
     6481 ctaaactctc ccttatatga ttatacgtca tattgtatat gctcttgttc tccgctgcat
     6541 atccgtatgc tttaaagccc agcctttttg ctatatacaa tgctctgtaa atatggaatc
     6601 tctgtgtgca tattattatg ctttgcactt tgaagatata gcccgctctg tatatgctgt
     6661 cataagtgtt gaatcccgca taatctctgt agatatcgtc tgcattcaca cctttggata
     6721 taaggtaatc atacatggcc tttgtttcgt tgtactcaac ggtgccgttg tcccccgaca
     6781 ctataacagc aatctttccg tcatagtatt catatacctg atatgcgtaa tccagcctgt
     6841 ctttaagcac gggtgaaggg tttccgcttg cgtatacagc cgcacccaac acaagaacgg
     6901 cctgaggtct ttcaatcttt cccaactgct caacactgtc aaatatatcg gacaaaggat
     6961 aaagcattac ataaagctgt aatgacgtaa acagcagtgc cgatatcagt aaaataaccg
     7021 ctattgcctt ttgtaatctg tttttctttt tcttttttct tttagaacgc attatccctt
     7081 actgcctgct ccagtttttt cttgtcttcc gccgtaagat tcttaaaagg ccttctcgcc
     7141 tctccgcaat caattcccgc aagttttgag acatatttca tagcctgaat agtagcaccg
     7201 ctttcaatca tgggttgtat aatgttgttc agttttgtct gtaatttttg tgccgaagca
     7261 atgtcttttt tgtcaaaggc ggcttttatc tgtacaaaag cctgcggata tatgttcatt
     7321 gttgttccga tagcaccggt tgctcccgcc attaaagcgg aagcgaaagc ttcatccttg
     7381 ccgctgtata tcggtttttt tgatgcaacc gctattctgt ttaacacata gtaatcggca
     7441 gcggtatatt taatggagta aatcctgtca tcggacaaca aatcgtttat ctggtctacc
     7501 gacatatcca cacccgtagc accgggaatg ttatatatca ttatctttag cccgcacccg
     7561 tccgctatat cggtatagta cttttttata gccttatgtc cgtgtgagta atagaacgga
     7621 ggaaccgaag ctaccgcatc aacgcccatg ctttttgcat gtttcgcata cattacggct
     7681 aacgccgtac cgggtgtgcc cacatgtgcc acaacagtcg ctccgtttgc ttcttttgcg
     7741 gctacttcca gcgttttcat tctttcttct tcagtcagaa gaaaggtctc cgcggaactg
     7801 ccggttgtat atagtccgtc ggctccttta tccaacacat atcttacaag ttttttcagc
     7861 gcctcataat ccacggtctg atctttttta aacggtgtga atatcggaac taaaatatca
     7921 catttcttca tgagcaaata cctcctgcta tgttaagttt aatggatact cgccttattt
     7981 acaatcaatg atgttatgtt ttatgataat atatattcaa atatattttg ttgataagaa
     8041 agaagaaaaa atgaaagatc cgagaaacga aaaactggca gatttattaa tcaactattc
     8101 ctgcagactt aaagaaggag agaagatact catttccgtc tccgagatcg ataacgacct
     8161 ggttaagagg ctggtggaaa aggcatatca ggcaaaagct ctgccttttg tacagatatc
     8221 cgactcgtcc ctgtccccga aactgtatga gcatataacg gaagagcagc tggaaataat
     8281 gaacgagcgg gacctgaact tcatgaagaa gatggatgca tatatagcca tacgggcaag
     8341 taaaaacata tatgagataa gtaccgttcc ttcggaaaaa ctgtccctgg cgtccagaat
     8401 attaaaacct tcctttgacc gaagagtcaa atatacaaag tggtgtgtgt tgcggtatcc
     8461 caatgagtcc atggctcagc ttgccggaat gaataccgaa aagtttaccg atttctatta
     8521 cgatgtatgt acgctggatt acaaaaaaat gagtgatgca atggataatc tggtggaact
     8581 tatgaacaaa accgataaag tgcatataaa gggagaaggt accgatttga ctttttccat
     8641 aaaggacatt cccgctataa aatgtgccgg tgaaatgaat atacccgacg gagaagtatt
     8701 taccgctccg gtaaaagact cggtaaacgg ttatgtaacc tttaacactc cttcggtaag
     8761 tgaaggtaag acatttgaaa atatccgtct ggaatttgaa aaaggtaaaa ttgtaaaagc
     8821 tacagccaac tataccgagc atttaaacaa gattttagat accgacgaag gcgcaaggta
     8881 tgtaggtgag tttgcaatag gtgtaaatcc gtatatatta gatcccatga aagacacttt
     8941 gtttgatgaa aagatatgcg gatctttcca ttttaccccc ggtgcctgct atgacgaagc
     9001 acccaacggc aatgattccg ccattcattg ggatttggtg tgcatacaga ccgaacagta
     9061 cggcggagga gaaatcatgt ttgatgacag gttaatacgc aaaaacggta tattcacggt
     9121 ggaagaactt cactgtctta accccgaaaa cttaaagtga ggactttatg aagataatgc
     9181 atcttgccgg cggcggtgat ataggaggag caaaaaccca tataatctct ttggcaaaag
     9241 agctttcccg taccaatact cttaaactgg tatctttccg atacggagat tttgccgaaa
     9301 gcgccaaagc cgaaggcatt gatgtgacgg aaattaaaag tgctttcctt ctcaaagata
     9361 ttcatgccct ttttattgaa tatgacaaat ttaaaccgga catagttcac tgtcacggag
     9421 ccaaagccaa catgatggga ctttttctta aaaggcatag aaatgcgtgt gttaccacca
     9481 ctatacactc cgattacaga aaagactata tgcataactt attcaaacag gccactatag
     9541 gtctgttcaa tacattggct ttgagaataa taaagtttca cattgccgtt accgaacagt
     9601 ttgcaagaac cctgatatcc agaggctttg aggcatataa catgttttcc atatacaacg
     9661 gcattgattt tgaaaaagaa atgcctaaga tatccaggca ggaatatttt gaacaatatg
     9721 gcataccgta taatcccgac gagttgatta tagggatagc ggcacgtctt accaaagtaa
     9781 aggatatacc cacccttata aaaggctttg caaaagctca tgccgataat tccgatatgc
     9841 gtcttcttat cggcggagac ggtgaagaaa agcaaaaact tacggatttg gcaaatcaac
     9901 tgggtgtaag tgaaaaggtg cactttttgg gctggataga cgatgtggac agctttttca
     9961 gtgtaataga cattgatgta atatcctcac ttagcgaaac tttcccttac tctttaaccg
    10021 aaggaatccg ggcgggagcc atccccgtat ccagtaatgt gggaggagca tcttcactga
    10081 ttgaccagaa agttaccgga tacatattcg agcccaaaga ttatgagaca ttaggcaatt
    10141 atctgtatca actgtcaaaa gacaagaata aacgcataac catgcaaaaa gcttcctatg
    10201 agcatacaaa agccaacttc tcgctaaagg caatgaagaa taaacaggaa gatatctatt
    10261 cgcttatact caaacacttt aacgaaaagg agcgcaacaa ggtagttata tgcggagcat
    10321 acgggcgagg taatgccggt gatgaggcaa taaaatacac aaccttatcc agtatgagga
    10381 atatagatcc ggatatggat atatgtgtaa tgaccttaag gcctaaaaaa gaaagagcaa
    10441 tcgacagagt tcagaccata tacaccttta atgttataaa gctgtttttc ctgttaagaa
    10501 aatccaagat atttgtaagc ggaggaggtt cccttattca ggatgtgaca tcatcaagat
    10561 cgcttttctt ctacaccttt actctttatc ttgccaagaa actgggctgt aaggttataa
    10621 tgttcggctg cggaataggg cctgtatcca gaccgttcaa cagaaagctg tcgggaaaga
    10681 taattgataa ctatgtggat gtaataacgg tacgcgatca tatgtcggaa aaagagttgc
    10741 aatctttagg agtaacaaaa cctcaaatca ccgtttccgc agaccccgca ataaacctga
    10801 ccgccgataa aagcaacacc cataagatat tcatttcgga agatataccc gcagacggaa
    10861 aatacatctg tctggcactt agaagctgga aaaacttcaa caggaatgaa gccgttgcaa
    10921 aagcctgcga gcatatgtat aacaaatacg gatttaccac aatattcttc cctatggaat
    10981 atcccaagga tttatctatg tcggaaaaga cggcggaact gttagataca ccttactatt
    11041 taattcgcaa taaatatccc accgagcagg tagtggcatt catgtcaaag atggaacagg
    11101 ttgtagcaat gagacttcac gcattgatat tcgcagccgc aaattatgtt cccgtagtgg
    11161 gaatatccta tgatgtaaaa gtatcgggtt tcatgaacta tatagaaagc tcggcatgct
    11221 tggaactttc cgatgtaacg gaacactccg ttattaatgc aatggaagag agttttaaac
    11281 atagggagag agttataaaa gcaacaaata cgttaaagga aaaagaagcg gtcaacaagc
    11341 agcagtttat caagttgttg gagaattaat aacactcaat cggttttttc ttccggaaca
    11401 agctttccgt ctttcatgca ggaacggata tttatcttcc cagcaacaaa aacagaaaca
    11461 ctgctactgt aactataagg atagcgataa ttatgaatac tgttattctt actttgactt
    11521 tgggtttttc tgtttccatt atctcacctc tcggtttaat aaaatatata cacggactta
    11581 aagccttata ttgcttctgt caatattttg attgtttaga tatgtttgat actatcataa
    11641 caggcattta tgtcaacgct ttgcaggaaa agttcattaa aagttcattt ttactaatat
    11701 tttagttaca ataagcgcta taaaatcgag tgttaccgat atttactttc ccgataatat
    11761 attctcaaat acatcattct tttctatatc cggaccgaca agacttctgt acaatccgaa
    11821 ccatctgcaa tcgtactgac tttcaccctg tcttacggca aaggaccata tgtgctgact
    11881 cttatgacag tcatagcctt gtacggccat ttcaaatgct gtaagcccgt tgaatcgctc
    11941 cagcggaata tcccagttca ttaaaatctt gttttcctca tataaatgaa gatacagttt
    12001 cggagtatcc catacaccgt atttttcata cgatgacaca tattttgtac tgtctgcggc
    12061 atattccacc gctaaagtaa gggcatatgc attcaacata tgagctccgt gaccgtactc
    12121 accttttaag tcatgtccta tcaccacttc cggtttgaat cttcttaaaa gctccacctg
    12181 gaaattcagc acatcctcca taggatatac gcttttggca tgttccagat ttgaacaata
    12241 tttatcttca aattcactta taacagggta tgcggtcatg cctacggtcc ataatccgtt
    12301 cagcaactca tgaggcctgt acggttctcc ccaatggttt gtaagatatg caatctggac
    12361 tttgtattgt aactgtccgg cataatacgg catcatacct ccgaagtaca gatgttcatc
    12421 atcggcatga gtgggtatca gcagcatatc ggcatcttca tacggaggat tccatatctg
    12481 tacccatccg ggaagattgc cgtatgtg
//
LOCUS       DAOPWC010000004        11719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_5601_length_11719_cov_4.848251, whole genome shotgun sequence.
ACCESSION   DAOPWC010000004 DAOPWC010000000
VERSION     DAOPWC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_11719_cov_4.848251"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PKJ65_00200"
     CDS             complement(<1..1719)
                     /locus_tag="PKJ65_00200"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNZ40287.1"
                     /translation="MDNKKRQDNIRDCNYDESQIQVLEGLEAVRKRPGMYIGTTSVRG
                     LHHLVYEIVANSIDEALAGRCDRIEITIDKGNIVTVTDNGSGIPVGIHPKMGIPTVEV
                     VHTVLHAGGKFDSSAYSISGGLHGVGASVVNALSEFLEVEVKRDGKIYKQRYERGKTV
                     TKLEIIGNTDETGTKTVFKADDRIFDTIEYEYDALLSRYREMAFLNKGVMIVLTDKRG
                     EEEITNELCYEGGIVSFVRYLDSNKTRIHKDTIYIEATKENCIVEIAMQYNDDYIETL
                     YSYANNIATSEGGMHVTGFRSALTKVINDYARKSGALKESDKNLTGEDVREGLTVVLS
                     VKLHDPQFEGQTKSKLGNSYVRTVTEQTVNEELAAYFEENPQEGKVIIDKCIRASQAR
                     EAARGARDLARRKTALEGNALPGKLADCSETDPVKSEIFIVEGDSAGGSAKQGRDRKY
                     QAILPLWGKMLNVEKARIEKVYNNDKLSPLITALGTGIGEDFDIEKLRYHKVIIMADA
                     DVDGAHIGMLLITFVYRYMRELIEKGHLYLAQPPLYRITKGKESIYAYNEAEKDRILR
                     ERDWDSK"
     gene            complement(1723..1992)
                     /locus_tag="PKJ65_00205"
     CDS             complement(1723..1992)
                     /locus_tag="PKJ65_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNZ40288.1"
                     /translation="MFLHIGDDLVVREKEVIGIFDFETSTLSKHTKNYLRDKEKKRTI
                     TTVSDKLPKAFIFVKKNKESDYRIYLTLISSATLRKRAENNKITE"
     gene            complement(2045..3151)
                     /gene="recF"
                     /locus_tag="PKJ65_00210"
     CDS             complement(2045..3151)
                     /gene="recF"
                     /locus_tag="PKJ65_00210"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNZ40289.1"
                     /translation="MLIERIVLKDFRNYEYQEIFPNKGINIIYGKNAQGKTNILESVF
                     LCSTARSHRTNKDADLIRTLQQGYSVKIEGKRDDEPFSIEINCTKISSRLNKRVIKVN
                     DITLSKTGQLMGVLNSVMFSPEDLTVIKEGPSERRRFLDILISQINTSYFYSLQEYNR
                     IIKQKNALLKRVYETGKNSDLIDVYNIKQAKTGAKIIKERAVFTEQINKKAGHNHFDI
                     ANRAEELEIKYSCDIPDYSGEESDFYKSLDKIKDKEIRIQSTIIGPHRDDIEILLNGL
                     NIRDYGSQGQQRSAILSLKFTEMQIIKEKTNKQPVLLLDDALSELDLLRKRKLLNKID
                     TKQTFITCTEKRRYMTLDDSISFFFVNKGSVKSI"
     gene            complement(3151..4257)
                     /gene="dnaN"
                     /locus_tag="PKJ65_00215"
     CDS             complement(3151..4257)
                     /gene="dnaN"
                     /locus_tag="PKJ65_00215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ40290.1"
                     /translation="MNFTCTRKKLQDAVNSVSKAISNNPIMPVLGNISIKAKDSQIIF
                     RGYDLEIGIEHIEDAFIKEEGEILVNARLFSDIVRSLGEEEVEIFTEDSKLVIISGNA
                     VFNIRWLDPAAFPKLPYVEKDNKVTIKTEYLKNMIRNTLFSVSTNEQRLILTGALFES
                     EENSFKMVTLDSFRMSIRKEYILGNKEEFNFVIPGKTLNELLKLITNDDEDIKIYYEK
                     TQVLFEFGNCKFISKLLQGDYFNYKAIIPTKDFATKIEISVNEFRNIIERNMLVSDDG
                     RKAPLILEISENILTANTQGEVGNVTEKCHIKKEGKDIRIGFNARYLLDAIKSIDEGK
                     VRISFMGETSACIITPLNSDEFLYLILPVRISAY"
     gene            complement(4459..5781)
                     /gene="dnaA"
                     /locus_tag="PKJ65_00220"
     CDS             complement(4459..5781)
                     /gene="dnaA"
                     /locus_tag="PKJ65_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ40291.1"
                     /translation="MTVQEIWNDALMNIESVMGELNFKTWILPLEPYTIDGDNFVLIA
                     QSDFSKNYLANYKLLLKNAISDAAAKDLSPLFKLKNEIGEFDENGKYEVFNSNNYANS
                     QLLDKYVFSNFVTGSSNQFARAAAEAVVDNPGTTYNPLFLYGESGIGKTHLMHAIGNA
                     ISRQYPDKKILYVQSETFTNEMISAIKTAKSKEFREKYRNLDVLLIDDIQFITGKEGT
                     QEEFFYTFEKLYYANKQIVITSDVPPDKMTRLEERLKTRFQQGLMADMQTPDYETRVA
                     ILEQNSLKSEYNISKEVIEFIARNISSNIRNLQGALNKTVAHARVFGEDITIDLAKKA
                     LKDMIDENNANDITIPSIIDTVARYYNITTKDIISPTRKSNIAFPRQIAMYICRIKTP
                     FSLPVIGKYFADRDHTTVLYACEKIAEKIKTDKIVEKEVNELLEKMAY"
     gene            complement(6098..7864)
                     /locus_tag="PKJ65_00225"
     CDS             complement(6098..7864)
                     /locus_tag="PKJ65_00225"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNZ40292.1"
                     /translation="MKMTKNKMLLSWIEEMAALTTPDKIVWITGDPKQLDELRKQALA
                     TGELYKLNEEKLPDCYLHRTAVNDVARVEHRTFICSRKEKDAGPTNNWMAPKEAYEKL
                     GKLFSKSMKGRTMYVIPYSMGPVGSKFSKIGIELTDSIYVVMNMVIMTRVGNDVLDAL
                     GNSNDFVRGLHGKADIDEENRYICHFPEDNTIWSVNSGYGGNVLLGKKCFALRIASYQ
                     GKNENWMAEHMLILGVEYPDGETKYIAAAFPSACGKTNLAMLIPPEVFAGKGYKVWTV
                     GDDIAWIRKGPDGRLWAINPENGFFGVAPGTNIKSNPNALHATEKGTIFTNVVHNLEN
                     NTVWWEGLDKNPPTKALNWKGEPWTAESGEKGAHPNSRFTAPATNCPSISKEFDSPNG
                     VPLSAIIFGGRRAKTAPLVYQSLDWVNGVFVGSIMASETTAAASGAVGVVRRDPMAML
                     PFCGYNMGDYFQHWLNIGKDVGNKVPKIFHVNWFRLDDEGHFLWPGFGENLRVLLWIL
                     KRCEDKVGAVETPIGYLPKPEDINLEGIDVTKETLKELLSVDKDLWLEDTVRIEEFYN
                     KIGDTVPKELWNELAKLKKNLT"
     gene            8046..9440
                     /locus_tag="PKJ65_00230"
     CDS             8046..9440
                     /locus_tag="PKJ65_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ40293.1"
                     /translation="MIKQFYQPEYMLRKHTKFGELPSAKEAYGRMFKMALPSTIEALL
                     GGLTSFVDMIMVSSVGITAVTAVGITTQPRMIILMLFMALNTGVTALIARRKGEDDAK
                     KANEVLMQALLICFVLAIIMTTIGMVTARPLLRFAGAGADFIEDSIIYYRIITAGTVF
                     GVLTMTINAAQRGAGNTKIAMTTSLISNVVNVVFNFLLIKGRLGFPALGVKGAAIATV
                     IGMVAASLAACYSLRNASFIYFDIEGLKKFDPEIVKSIANISGNAAFEELCFRFGFFM
                     YNKIVAELGTIDYGTHLVCMQIMNLSTYIGAGLGIGTTALVGQTLGEKRSDLSMLYIK
                     IGRRMAIIISVVLSAVFIFGGKMLMGLFTDDPVIIENGRNILLIMALSSFCINTQIVH
                     SGSLRGAGDVKFVATMALISVAVIRPLFSYILVYPLQLGLIGAWLGLLADQFVRSFSG
                     MLRIRRGKWATIHI"
     gene            9469..10608
                     /locus_tag="PKJ65_00235"
     CDS             9469..10608
                     /locus_tag="PKJ65_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNZ40294.1"
                     /translation="MFAKIIQLVLFFGIPYVLYKLEHKVKLIKTVGPIIFCYAIGIIW
                     ANSGLPLDAGLSDTVSSVLIPVAITLILFNSDFLTLKKNVKKAGLSFLILSVSVMVVA
                     AVSVLVFPNVFEKEKIASMLIGCYTGGTPNLFAIGYGLNADKNTIIVANLADQVLGGV
                     YFLFICTLAKPLLNKLSGQISVATEKTDYQKEGIVDKAEIPLKEKIKQGTIAILLGIA
                     ALGVSIGISFLLTKEATNVAIIMLGVSVIGVALSFVKGVRQNKVSYSIGQYFIYVFSL
                     AVGSMANLKLILGQSVYVLGMVAFILFVTMFLHFIISLLFKIDTDTIIITSVAGIFGP
                     AFIIPTAKAIKNEGVILIGIATGLLGYAIGNFMGFGLYELLKLIY"
     gene            10619..>11719
                     /locus_tag="PKJ65_00240"
     CDS             10619..>11719
                     /locus_tag="PKJ65_00240"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HNZ40295.1"
                     /translation="MGFKDLEIKELVKNTYPCECGLVHDPKMERIEISEYGIEEAIRF
                     ILNKAKGRIMVISDEITFEIAGRTVLEKLKETKKDTVHYIMPQRNNIPVVPDDGTIGE
                     TLIRMPLGIDFFVAVGSGVVNDIVRIISYHLAKEYAVIGTAPSMDGYASTTSSLIINN
                     IKESCQGQVPTGIFADINILQNAPYIMLTAGFGDVLSKYNAVREWRFSRDLNREHYCE
                     EVAGLVWKAADKCVDSASLLKKRDKNAVVNIMEALILSGIAMGMYKNTRPGSGSEHHL
                     VHYWDIEFIKRGWIHPLHGNAVGVALCVICRLYDLIKEKLPVEITDLNTENIENILKS
                     AGCETNPKTLGIPEDIFHDSILYGHTMSSSKYT"
BASE COUNT         3254 a         2314 c         2141 g         4010 t
ORIGIN      
        1 tttactgtcc cagtctcttt cgcgcaatat tctgtctttt tccgcctcat tatatgcata
       61 tattgattct ttccccttgg ttattctgta taaagggggc tgtgcgaggt aaagatgccc
      121 tttttctata agttcacgca tatatctgta tacaaaagta atcagaagca ttccgatatg
      181 agcaccgtca acatccgcat cggccattat tatcacttta tgatatctca gcttttcaat
      241 atcaaaatct tcgcctattc ccgtgcctaa tgccgtaatt aaaggagaaa gtttgtcatt
      301 gttatatact ttctctattc ttgccttttc cacattgagc atttttcccc ataaaggaag
      361 tattgcctga tattttctgt ctcttccctg tttggcgctt cctccggcac tgtctccttc
      421 cactataaat atctcgcttt ttacaggatc ggtttccgaa cagtctgcaa gctttccggg
      481 aagtgcgttt ccttccaatg cggtttttct tctagccaaa tcacgggcac ctcttgctgc
      541 ttctctggcc tgggatgcac gaatacattt gtcaatgatt acttttccct cttgcggatt
      601 ttcttcaaaa tatgcagcca attcctcatt cacggtttgt tcggtgacgg ttcttacata
      661 actgttaccc agttttgatt tagtttgccc ttcaaactgc ggatcgtgta atttcaccga
      721 taatactacg gttaaacctt ccctcacatc ttctcctgtc aggttcttat ccgattcttt
      781 taatgctccg ctttttcttg cataatcatt tattactttt gttaaggctg atcggaaacc
      841 ggtaacatgc attccgcctt cactggtcgc aatattgttt gcatatgaat acagtgtttc
      901 aatatagtca tcattgtact gcattgctat ctccacaata caattttcct ttgtcgcttc
      961 tatgtatatt gtatctttat gaattcttgt tttattggaa tccaaatacc ttacaaagga
     1021 tactatgccg ccttcataac ataattcgtt tgttatttct tcctctcctc ttttatccgt
     1081 taacactatc ataacgccct tattaagaaa cgccatttct ctgtatcttg ataaaagagc
     1141 atcatattca tattcaatgg tgtcaaaaat ccggtcatct gctttaaaga cggtttttgt
     1201 tcctgtttcg tcagtatttc caattatttc cagttttgta actgtttttc ctctttcata
     1261 tctttgtttg tatatttttc cgtctctttt tacttccact tcaagaaatt cgcttaatgc
     1321 gttaaccacc gaagctccta caccatgaag tcctccggat atactatatg ccgaagagtc
     1381 aaactttcct cccgcatgta atacagtatg aacaacttcc accgtaggta ttcccatttt
     1441 cggatgtatt cctaccggaa ttccgctgcc gttgtcggta actgtaacta tattgccttt
     1501 atctatcgtt atctcaattc tgtcacatct tccggcaagt gcttcatcaa tactgtttgc
     1561 cactatttca tagacaagat gatgtaatcc tcttacggat gtagttccta tatacatacc
     1621 gggtcttttt ctgaccgctt caagaccttc cagtacctgt atctgcgact cgtcgtaatt
     1681 gcaatctctt atattgtcct gtcttttctt attatccata tattactccg ttattttatt
     1741 gttttcagct cgttttctta gcgttgcaga agatatcagt gtgagatata tccggtaatc
     1801 ggactcctta tttttcttaa caaaaataaa agctttcgga agtttgtcac ttactgttgt
     1861 tattgttctc tttttttctt tatctctgag ataatttttt gtgtgcttgg acagtgtaga
     1921 agtttcaaaa tcaaatattc ctattacctc tttttcgcgc acaaccaaat catctcctat
     1981 gtgtaaaaac atgaaatcct ctcttcattc ttctgttaat tatagcattt ttgcggtctt
     2041 atttctatat acttttaacc gatcctttgt ttacaaagaa aaagcttata ctgtcatcca
     2101 gggtcatata tcgtcttttt tcggtgcagg taataaaagt ctgttttgta tctattttat
     2161 ttaataattt tcttttcctt aataaatcca gttcactcaa cgcatcatcc agcaataata
     2221 ccggctgttt gtttgtcttt tcttttataa tctgcatttc cgtaaacttt aaagacaata
     2281 ttgcagagcg ctgctggccc tgggacccgt aatctcttat atttaatccg tttaataata
     2341 tctcaatatc atctctatga ggccctatta ttgtactttg aatccttatt tccttgtctt
     2401 ttattttatc caatgattta tagaagtcgc tttcctcccc tgaataatcg ggaatatcac
     2461 aggaatattt tatttccagt tcttctgctc tgtttgctat atcaaaatga ttatgtccgg
     2521 cttttttatt tatttgttcc gtaaaaactg ctctttcttt tataatttta gctccggttt
     2581 tagcttgctt tatattatat acatcaataa ggtcgctgtt ttttcctgtt tcatacactc
     2641 tttttaaaag agcgtttttt tgttttatta tcctattata ttcctgtaat gaataaaaat
     2701 atgatgtatt aatctggctt atcaatatgt ctaaaaatct tcttctttcg ctcggacctt
     2761 cctttataac ggtaagatct tcgggcgaaa acataacact gtttaaaacc cccatcagtt
     2821 gccctgtctt tgaaagggtt atatcgttta cttttataac tcttttattt aatctggagg
     2881 atatcttcgt gcaatttatt tctatggaaa aaggttcatc gtctcttttt ccttcaattt
     2941 ttaccgagta tccttgttgt aaagtcctaa taaggtcggc atctttattt gttctgtgtg
     3001 atcttgctgt ggaacataaa aataccgact caagtatatt ggtctttccc tgagcatttt
     3061 ttccgtatat tatattaatc cctttattcg ggaatatttc ctgatattca taatttctga
     3121 aatctttcag tacaattctt tctattaaca ttaataagca gatatacgta ccggaagaat
     3181 aagataaagg aattcgtcgc tgttaagggg ggttataata cacgcacttg tttctcccat
     3241 aaaagatatt cttaccttac cttcatctat tgatttaatg gcgtcaagta aatatctggc
     3301 attaaatcct atcctgatat cctttccttc cttctttata tgacactttt ccgttacatt
     3361 acccacttca ccctgtgtat ttgccgttag tatattctcg cttatctcta atataagggg
     3421 agcttttctt ccgtcatccg atacaagcat atttctttcg ataatattcc ggaattcgtt
     3481 tacacttatc tcaatttttg tcgcaaagtc ttttgtagga ataattgcct tataattaaa
     3541 ataatcaccc tgtaaaagtt ttgaaataaa cttacaatta ccgaattcaa acagtacttg
     3601 cgttttttca taataaattt ttatatcttc gtcatcattt gttattaatt taagtaattc
     3661 gtttaatgtt tttcccggta ttacaaaatt aaactcttct ttattaccta atatatattc
     3721 ttttcttata ctcattctga aactatccag agttaccatc ttaaaactgt tttcttcgct
     3781 ttcaaacaaa gctccggtta agataagtct ttgctcgttt gtactgacgg aaaataaagt
     3841 gtttcttatc atatttttaa gatattccgt tttaatggta actttattat ctttttccac
     3901 atatggtaat ttaggaaatg cagcaggatc caaccatctt atattaaaca cggcatttcc
     3961 cgaaataata accagtttac tgtcttcggt gaaaatttcc acttcctctt ctccaagact
     4021 tcttactatg tcggaaaaca accttgcatt aaccagtatt tcaccttctt cttttataaa
     4081 tgcatcttca atatgttcta ttcctatttc aagatcatat cctctgaata ttatctgaga
     4141 atctttagcc tttatactta tatttcccag tacaggcatt ataggattat ttgaaatagc
     4201 ctttgacaca ctattaacag catcttgtaa tttttttctt gtacatgtga aattcattta
     4261 gtttcccctc tttcttttaa taacatatct ataagcagta gtagtaggtc ttgtgaaatt
     4321 tgtgtataac gtattttatc ccttatttat cgctcttttt actgtggaca atttttgtga
     4381 ataaaaacag taaactacgc ttatatgtaa attatacaat attccacaga aagatattaa
     4441 catttacaca gaaaattgtt aataagccat cttttccaat aattcattaa cttctttttc
     4501 tacaatttta tcagttttta ttttttccgc tattttttca caagcataca atacggtagt
     4561 gtgatctctg tccgcaaaat atttgcctat taccggaaga gaaaaaggcg ttttgattct
     4621 gcatatatac atagctatct gtcgcggaaa agcaatatta cttttccttg tcggagaaat
     4681 tatatctttc gtcgttatat tgtaatatct tgcaacggta tcaataatgc tcggaattgt
     4741 aatatcgttt gcattatttt catcaatcat atcttttaat gcctttttgg caagatctat
     4801 ggttatatct tccccgaata cgcgcgcatg agctaccgtt ttatttaatg ctccctggag
     4861 gttccttata tttgaagata tattccttgc aataaattct attacttcct tggatatgtt
     4921 atattccgac tttaaagaat tctgttcgag aatagcaact ctggtttcat agtccggtgt
     4981 ttgcatatcc gccataagtc cctgctgaaa tctcgtttta agcctctctt ccagtctggt
     5041 cattttatcc ggaggtacat cggaagtaat aactatctgt ttgtttgcat aatacaattt
     5101 ttcaaacgta tagaaaaact cttcctgtgt tccttctttt ccggtaataa actgaatatc
     5161 atctattaaa agaacgtcca gatttctgta tttttctctg aattctttac tttttgccgt
     5221 ttttattgcg gaaatcatct catttgtaaa agtttcactt tgaacatata agattttctt
     5281 atcaggatat tgtcgggaaa tcgcatttcc tatagcatgc ataagatgag tttttcctat
     5341 tccgctctct ccgtatagaa ataagggatt atatgttgtg ccgggattgt ccacaacagc
     5401 ttccgctgcc gctctggcaa actgattgga agagccggta acgaaattcg agaagacata
     5461 tttatccaga agctgggaat tggcataatt gttggaatta aaaacctcat actttccgtt
     5521 ttcgtcaaat tctcctatct cgtttttcag tttaaaaagg ggactgaggt cttttgcggc
     5581 cgcatcggag atggcatttt ttaaaagcag cttgtaatta gccagatagt tttttgaaaa
     5641 gtccgattga gcaatcagta caaaattgtc tccgtcaata gtgtaaggtt ccaatggaag
     5701 aatccatgtc ttaaaattaa gttcacccat tacactctca atattcatta aagcatcgtt
     5761 ccatatttct tgtacggtca taaatgttag tttcccctaa ttattaaaaa agataaagcc
     5821 gctttttgaa atgtttcgct ccaagcagat ttatctttcg tggcctgtgg ataaaaatga
     5881 tgttatcaac agggaagcat agtatatcaa agaagcaaac acagcacaag gggaaaaaag
     5941 aagaaatttt aaaaaaattt aaccggattt ttaacttatc cacagaaagt cacttatcca
     6001 cagaaaatct gttgataact taaaggattt tatcttttct gcgtataaaa aaggttgtgc
     6061 cctgtaagca caaccttctg aaatttgccg gaatacctta tgtaagattt ttcttaagtt
     6121 tagctaattc attccaaagc tctttcggta cggtatctcc gattttattg tagaattctt
     6181 ctattctgac agtatcttca agccagaggt ctttatcaac agaaagaagt tcctttaatg
     6241 tttcctttgt aacatcaatt ccttcaaggt ttatgtcttc aggttttggg aggtagccga
     6301 tcggagtctc tacggcaccg actttatctt cgcatctttt cagtatccaa agcagaacac
     6361 gcaaattctc tccgaatccc ggccacagaa aatgaccttc atcatccagt ctgaaccagt
     6421 ttacatggaa tatttttgga accttgtttc ctacatcctt tcctatattc aaccagtgtt
     6481 gaaagtaatc acccatgtta taaccgcaga aaggaagcat ggccatagga tctcttctta
     6541 cgacaccgac agctccgctt gcggctgccg ttgtctcgga agccattatg gatcctacaa
     6601 acactccgtt tacccagtca agagattgat ataccaaagg agcggtctta gctcttcttc
     6661 cgccgaatat tatggcggaa agaggaacac cgttcgggga gtcaaattct ttacttatgc
     6721 ttggacaatt tgttgcggga gcggtaaatc tgctgttggg atgtgctccc ttttcgcccg
     6781 attcggctgt ccagggttca cctttccaat tgagagcctt agtcggagga ttcttgtcta
     6841 atccttccca ccaaaccgtg ttattttcaa gattatgaac aacatttgtg aaaatcgtgc
     6901 ctttttccgt tgcatgcaat gcgttgggat tggattttat gtttgtaccc ggagcaactc
     6961 cgaagaagcc gttctccgga ttgattgccc aaagccttcc gtcgggacct tttcttatcc
     7021 atgcaatatc atctcccacg gtccatactt tatatccctt cccggcaaaa acttccggag
     7081 ggataagcat tgcaagattg gtctttccgc aagctgacgg gaatgccgcc gctatgtact
     7141 tagtctctcc gtcgggatat tcaacaccga gaatgagcat gtgttccgcc atccagtttt
     7201 catttttacc ctgataagag gctattctta aagcaaaaca ttttttacct aaaagaacat
     7261 ttccgccgta accggagtta accgaccata ttgtattgtc ttcaggaaaa tgacagatat
     7321 atctgttttc ttcatctatg tccgctttac cgtgaagccc tctgacgaag tcattggaat
     7381 ttccgagagc atcaagcaca tcattgccta ctctggtcat aattaccata ttcattacaa
     7441 catatataga gtcggtaagc tcaataccga tttttgaaaa cttggagccg acaggtccca
     7501 ttgaataggg aataacatac attgtccgcc ccttcatgct tttactgaag agttttccca
     7561 atttttcata tgcttctttg ggtgccatcc agttatttgt aggaccggca tctttttctt
     7621 ttctggaaca tatgaatgta cgatgttcta cccgtgccac gtcgtttacc gccgtgcggt
     7681 gtaaataaca gtcgggaagt ttttcttcgt tgagcttata aagctcaccg gttgccaagg
     7741 cttgttttct taattcatca agctgtttag gatctcccgt aatccagaca attttatcag
     7801 gagtagtaag agccgccatc tcttctatcc atgaaagtag cattttgttc tttgtcattt
     7861 ttatatctcc ttcctttcgg aaattaaatc ggttttcaaa aactccctcc tttaatatta
     7921 attcacttca cggaatatgt aaagacaaaa agccgatcag gagagaaaaa tacagtaaga
     7981 cggagattag attttaaaga gatattatat gaccaaacaa gtaaaagcat ggtaaaataa
     8041 agagcatgat taagcaattc tatcagcctg aatatatgct tcgtaagcac acaaaatttg
     8101 gggaacttcc ttcagcaaag gaagcatacg gcagaatgtt caaaatggca ttgccgtcta
     8161 caattgaagc gctcctggga ggattgacca gttttgtgga tatgataatg gtatcttccg
     8221 tcggcataac agcggttacg gcggtaggaa taacaacgca gccgagaatg attattctta
     8281 tgctttttat ggcattaaac acgggagtta ccgctttgat agccagaaga aagggagagg
     8341 atgatgccaa aaaggcaaat gaggttttaa tgcaagctct tctgatatgt tttgtacttg
     8401 caattattat gacaacaatc ggaatggtta cggcaagacc gcttttgcga tttgccggag
     8461 caggagccga ttttattgag gattcgataa tatattaccg gattattact gcaggcacgg
     8521 tattcggtgt tcttaccatg accataaatg cggctcaaag aggtgcgggt aatacaaaaa
     8581 tagctatgac cacaagcctt atatccaatg tggtcaatgt agtttttaat ttcctgctga
     8641 taaaaggaag attgggtttt cctgccctgg gagtaaaggg cgccgcaata gccactgtta
     8701 taggtatggt tgccgcatct ttagcagcat gttattcgct gaggaatgcc tctttcattt
     8761 atttcgacat agaaggcctg aaaaagtttg atccggaaat tgtgaagtcc attgctaaca
     8821 tttcgggaaa tgccgcgttt gaagaattgt gctttcgttt cggcttcttt atgtataaca
     8881 aaatagttgc ggagctggga accatagatt acggaacaca tcttgtgtgc atgcagataa
     8941 tgaatctttc tacatatata ggagccggcc tgggaatagg aacgacggct ttagtgggac
     9001 aaactttagg agaaaagcga agcgacctct ccatgcttta cattaaaata gggagaagga
     9061 tggcaataat catttccgtt gtcttatcag ccgtatttat cttcggagga aagatgttga
     9121 tgggcctgtt taccgatgac cccgtgatta tagaaaacgg aagaaatatt ttgttaatta
     9181 tggcactgtc ttctttctgt ataaatacac agatagtaca ttcgggaagt ttaaggggag
     9241 cgggggatgt aaaatttgta gcgaccatgg cgctgataag tgtagcggtg atacgcccgt
     9301 tattttccta catattggta tacccgttgc agctcggcct tatcggagca tggctcggcc
     9361 ttcttgccga ccagtttgtc cggagtttct cgggaatgtt aagaataaga agaggaaaat
     9421 gggcaactat acatatttag tatataattg tttcgggagg gggaatccat gtttgcaaag
     9481 ataatacaac ttgttttatt tttcgggata ccttatgtgc tgtataagct ggaacataaa
     9541 gtgaaactta taaaaacggt cggcccgatt attttttgtt atgcgatagg aataatatgg
     9601 gcaaacagcg gattacctct ggatgccgga ttatcggata ccgtgtcatc ggttcttata
     9661 cccgtcgcaa taactcttat actttttaac tcggattttc tgacattaaa gaagaatgta
     9721 aaaaaagccg gactgtcttt tctgatttta agtgtttcag taatggttgt agccgctgtc
     9781 agtgttttgg tttttccgaa tgttttcgaa aaagaaaaaa tagcctccat gctgataggg
     9841 tgctataccg gcggtactcc aaatctgttt gcgatagggt acggactgaa tgccgataaa
     9901 aacaccatca tcgttgccaa tcttgcggac caggtactgg gaggagtgta ttttctgttt
     9961 atatgcacat tggccaaacc cttgttgaac aaattgagcg ggcagatatc cgttgcaacg
    10021 gaaaagaccg attatcaaaa agagggtatt gtagacaaag cggaaatacc gctgaaagaa
    10081 aagataaaac agggaacaat agccatcctg ttgggaatag cggcactcgg tgtaagtata
    10141 gggatttctt tcctgctgac aaaagaggct accaacgttg ctataatcat gctcggagta
    10201 tcggttatcg gagtggcatt atcctttgta aagggggtaa ggcaaaacaa agtatcctat
    10261 tccatagggc aatacttcat atatgtattt tcattggctg tcggctccat ggccaacctg
    10321 aagcttatac tggggcagtc ggtgtatgtg ttaggcatgg tggcttttat actttttgta
    10381 acaatgtttt tgcactttat tatttcactg ctctttaaga ttgatacgga cacaataata
    10441 atcacatcgg tagcggggat attcgggcct gcgtttatta tacccacggc caaagccata
    10501 aaaaacgaag gggttatact tatagggatt gcgaccggat tgttgggcta tgcgatagga
    10561 aacttcatgg gtttcggatt atatgaatta ttgaaactta tttactagag ggaagaaaat
    10621 gggatttaaa gatttggaaa taaaggaact tgtaaaaaac acatatccgt gtgagtgcgg
    10681 gttggttcat gatccgaaaa tggaaaggat agagatatcc gagtacggca tagaagaagc
    10741 aatcaggttt attcttaaca aggcaaaagg caggataatg gtaatttccg atgagattac
    10801 tttcgaaatt gccggcagaa ccgttttaga gaagctgaag gaaacaaaaa aagatactgt
    10861 ccattatata atgcctcaga gaaacaatat ccccgttgta cccgatgacg gcaccatagg
    10921 cgagacattg ataaggatgc ctttgggaat agactttttt gttgccgtgg gaagcggagt
    10981 ggtcaatgat atcgtaagaa ttatctccta tcatttagca aaagaatatg cggtaatcgg
    11041 aaccgctcct tccatggacg gatatgcttc aacaacatcg tcgctgataa taaacaacat
    11101 aaaagaaagt tgtcaaggac aggtaccgac aggtattttt gccgacataa atatactgca
    11161 aaatgctcct tatattatgc ttacggccgg tttcggggat gtattgtcaa aatacaatgc
    11221 agtcagagaa tggaggtttt ccagagactt aaacagggag cattactgtg aggaagttgc
    11281 aggattggtt tggaaagctg cggacaaatg cgttgacagc gcctctcttt tgaagaaaag
    11341 agataaaaat gcggtggtca atattatgga agcacttatc ctgtccggta tagcaatggg
    11401 catgtacaaa aataccagac cgggttcggg atcggagcat catctggtac attattggga
    11461 tattgagttc ataaaaaggg gatggattca tcctttacac ggaaatgccg taggtgtggc
    11521 attatgcgtg atttgcagac tgtatgattt aataaaagag aaacttcccg ttgaaataac
    11581 ggatttgaac acggaaaata tagagaatat cttaaagagt gccggttgtg aaacaaaccc
    11641 gaagacttta ggcattccag aggacatttt ccatgacagt atcttatacg gacacacaat
    11701 gagttcttca aagtacacc
//
LOCUS       DAOPWC010000005        10784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_6189_length_10784_cov_3.078292, whole genome shotgun sequence.
ACCESSION   DAOPWC010000005 DAOPWC010000000
VERSION     DAOPWC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6189_length_10784_cov_3.078292"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..696
                     /locus_tag="PKJ65_00245"
     CDS             106..696
                     /locus_tag="PKJ65_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage infection protein"
                     /protein_id="HNZ40296.1"
                     /translation="MKYDYIDKILADNDGILKTADIVAAGICKETFYNYAKDTELEKA
                     AHGIYISPDVPVDEMFLLQTQIPKVIYSHETALYLHNLSEKEPIPLTVTVPAKYNNPT
                     LTKKGVKIVYVKKEWYSLGICQMSSPAGYDITAYDMERAICDVVRKRSYMDISAFNYA
                     LTEYMKQKGKDLNRLMEYAKIMRLEKRIRETMGVLF"
     gene            complement(732..1025)
                     /locus_tag="PKJ65_00250"
     CDS             complement(732..1025)
                     /locus_tag="PKJ65_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ40297.1"
                     /translation="MNYQVIFTQAAFRDLKKIDSYTAAMIMGWIRKNLEGCSNPRQHG
                     KSLTAAHSGKWSYRIGCYRLLADIQDDKILILILNVGDRKEIYKKTSTVNETF"
     gene            complement(1022..1243)
                     /locus_tag="PKJ65_00255"
     CDS             complement(1022..1243)
                     /locus_tag="PKJ65_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HNZ40298.1"
                     /translation="MTISIRLNDTDAQLIRKYAEMKGMSISELVRQSVIERIEDEFDL
                     KVYEATLAEYKANPVTYTLDEVEKELELR"
     gene            1776..3074
                     /locus_tag="PKJ65_00260"
     CDS             1776..3074
                     /locus_tag="PKJ65_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40299.1"
                     /translation="MYRTSLEYLYKWKEKDNRKPLIIRGARQVGKTWLMKEFANKAYK
                     SHAYINFDNNIQIKQLFLQDLNVDRLIMGLELYTGKKIVPSETLIIFDEVQEVPDALT
                     SLKYFNEDAPSYHIVCAGSLLGIALHPGTSFPVGKVEFMDLYPLSFSEFMLAMGKEQY
                     AELLESKDYEMITTFKQEYINLLKQYFFVGGMPEVVAHYVDNKDFQGVREIQRRILDA
                     YEQDFSKHAPNEIVPRIRMLWNSIPAQLAKENKKFIYGLIKEGARAKEYETALLWLTD
                     CGLIHQVNRVNAPNIPLKAYEDLKAFKLFTLDVGLLSCMVGLHQSALLSGNDLFREFK
                     GALTEQYVLQQLKTLDNLNVYYWTNDRNRAEIDFLIGSEGRVIPVEVKAEVNLQSKSL
                     KTFRDKYDIEISVRTSMSDYKKEERLINLPLYAISNIKKI"
     gene            3175..4638
                     /locus_tag="PKJ65_00265"
     CDS             3175..4638
                     /locus_tag="PKJ65_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNZ40300.1"
                     /translation="MGRDLRKLTLLHSNDMHGDFMSENIDDKLVGGVAMLSGYIDKVR
                     QEEENVVYVIAGDMFRGSIIDQEYRGLSTIEIVNMLSPDVVTLGNHEVDYGVAHLLFI
                     EKLAKFPIINSNLYIKTNHTRLFRPYLIKEVGGIKILYIGILTEEVIAQTKQETLIGS
                     IVDVYEAAKEVGRICNAYSQIDIDFTILLTHIGFEEDKKLASMLDPAWGVDIIIGGHS
                     HTYLEQPEIVNGIVIAQAGTGTDQIGRFDIIVDKDNNCIEDFTWQAVPIDETHCPVDK
                     DLEEIIHQYHEKTSEKFSRIITRFKRALTHPKRSQETELGNLCADAVVDNLGLDIMLF
                     ASGSIRKKELGPILTLKDFKECFPFDDKIYRIKLTKKQIEKAFNTILGRDLSDHVEFY
                     QVSRGFKVKYSKKENRVTEISLESQPLDDNHIYSVGLQEYHFNNMADFIGITKEEAEA
                     NSPIKVVTTSSGDVLEEAFLGMVLADPKVEGRIVIEY"
     gene            complement(4769..5041)
                     /locus_tag="PKJ65_00270"
     CDS             complement(4769..5041)
                     /locus_tag="PKJ65_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNZ40301.1"
                     /translation="MPRFDGTGPRGMGPLTGRAMGYCTGARRPVGRYYGRGGGRGRGF
                     GVCNRLYYYDDAPLYDEKPSKELLLNQKKFLEEELASINKQIDEYE"
     gene            5228..6352
                     /locus_tag="PKJ65_00275"
     CDS             5228..6352
                     /locus_tag="PKJ65_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40302.1"
                     /translation="MSLTSKERLMRIFANKPIDRPAIKLWGSWNYRKGDKLLHPSYEP
                     AMIKAAEKTDLVVNAHSPFFMFCGQVDIVRRKNVEINDPLWYEEHIVFHTPMGQLRRV
                     DRISKIGEPGFTIEYPVKQPEDIKRMLSMPYEPFPYVDNIEEQQQLLGDRGIVLYRID
                     HIAYALYRMIGSENLAYFTHDCRDLLMEALDVFSKRLYAHVEEALNHGVRVPFSWVGP
                     ELLIPPLLSEKDFMDFCFDYDKHICDMVHNAGSYIWVHCHGKVRNLIEKFIEMGVDVL
                     NPLEPPKNGDVDLDEVARTFKNRIGLEGNIEMQDFYDESEEVIREKIKTCVQSGKQSN
                     RFIMCTSAGFMDFPTPSKQYLDNIMTYIDYGYECVNNARY"
     gene            complement(6387..8522)
                     /locus_tag="PKJ65_00280"
     CDS             complement(6387..8522)
                     /locus_tag="PKJ65_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40303.1"
                     /translation="MKRNKQTKIDFGYAFSEPHRICVCLPNSSNKTLYDCTKDSLTML
                     WSDSDTTHNPLGAYKRVRLDWKVKITAKCNDVAMEAFAWERIEGYIPSLLYKANAEGA
                     NLTVQCVATRLGDVIKMSVTNTTGSDKKIFIDACVLDNTINTKWADFDCEYSVVNPIF
                     NDASDRIVIMDASLTDTVPSRRESVTYAYILKAKETRESMLIRPNKKYNDDIEVMLKT
                     NWNALYELGLNEWRNIIKKAPVFDLPDPMIENAYKACLCDIFVMREEMADGRMAGLAG
                     TEMYRSSNTGEPCFQALTLGKYGFFEPAKENIEFVASFQLNNGSWEDDKQWGKYMWAS
                     SGWKSRCIQHYYLRTKDRAFLEENFERMLASARWSKAQRDKTKRKNPPDSPEWGLMPR
                     GMGDGGLKNGDDLFGVFYPHNFMHCMGLEAAVWAAKELQREDVYQELLDTYLDHKECI
                     LKSLEIGHIKEPDGSIWIGSCAHNTSGSRWAVADAVYPTNILPVDHPLAVGTMNKLQG
                     NISEGGLPKNLGWIHDGLWVAIALDAMAYVNILADKADISADYLVAALNHGTPLFTWC
                     EERTEEKGAKRITGDLQHAWTPICVTQFIRDMLVSDYLFKDDMLHLTCAVPRYFYKEG
                     NVMEVKEAPAFQGKISFNIEVSKNTVTFTADLSSFDINRELCLHLRLPNRNLEVTVQS
                     ISGGSAVITEQKAVIKPDTNKIKAVFSIG"
     gene            complement(8550..9347)
                     /locus_tag="PKJ65_00285"
     CDS             complement(8550..9347)
                     /locus_tag="PKJ65_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ40304.1"
                     /translation="MIIDFHTHIFPDTLAKKARETLLRNVNYRYEPTTDLTKAGLMKT
                     MDQWGIDISVVQTVITKSTQLQNTNLWAQSVCDDRIISFGGIFPHTEDYKKDIDFVCG
                     LGLKGIKFHVEYQAFVVDDPKALRIYDYALSKGLIIVHHAGEDHGMPPPYRSNPQKFA
                     YIADHMQGGVMVAAHLGSHNMWDDVERYLVGKNIYLDTSMGFEYYSKEQFLRIVKAHG
                     ADRILFASDSPWSNAKNEIGAVNSLPINQTSKDLILGGNAARILGLS"
     gene            9571..>10784
                     /locus_tag="PKJ65_00290"
     CDS             9571..>10784
                     /locus_tag="PKJ65_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40305.1"
                     /translation="MKPELLKLREYAKQYAEIALSDRNLAIPEQYRKLNSLQQVRPPV
                     LVFEVPWGELEDQEKLRLECETDLYRGIERAVKRALYQFEYFRGDYAIHPYYKVRIRT
                     KGTGIGLTVQEVMIGSATGTDISAHEYHDILPDEESLEKITMPTMQVDEQATQKALQA
                     AHDVFDGIMDVGLGGYQLIFNMWDEIPRYHGVENCLIDIYERPDFIHKMMDKFTRYYE
                     ETVSLYEKLNILETDAYYLHCTPACTYELPVKDVTEETVSAKDVWGRGMAQIFAVVSP
                     DMHKEFDIKYMKRLFDRCGLTYYGCCEPLHNKIDILTEFDNLRRISVTPWADVDIAAN
                     AMGNRYVLSAKSNPAYVSQAVFDPQPVIEETKKILLACKRNNTPCEFVLKDISTVNRN
                     VNHLSEWVKTVS"
BASE COUNT         3250 a         2008 c         2263 g         3263 t
ORIGIN      
        1 aacggttata ccgttcactt atttagaaga aagaaaatcc tataatgcat tgacttttat
       61 acggtcttta ggataaaata tccgtatgaa agttggtgat ttttaatgaa gtatgactat
      121 attgacaaaa tcctggcgga taacgatggt atcttgaaaa cggctgatat tgttgctgca
      181 ggtatttgca aagaaacttt ctataattac gcaaaagata cagagcttga aaaagcggct
      241 cacggcattt atatttcacc ggatgtgccg gtagacgaaa tgttcttact gcaaactcag
      301 attccgaaag tcatatattc ccatgaaact gcactgtatt tgcacaatct ttccgaaaaa
      361 gaaccgattc cgcttactgt aacggtgcct gcgaagtata acaatccgac attaacaaaa
      421 aagggtgtaa aaattgttta tgtaaaaaag gaatggtatt cgcttggtat ctgtcaaatg
      481 tcctcgcctg caggatatga tattactgca tacgatatgg aacgcgctat ttgcgatgta
      541 gtccgtaaac gatcttacat ggatatttcc gcttttaact atgctttaac tgaatacatg
      601 aaacaaaagg gaaaagacct taaccggctt atggaatatg caaaaataat gagactggaa
      661 aagagaattc gtgaaacaat gggggtattg ttttaattaa ataaatttag agttaatagt
      721 tgtaacccgg attaaaaagt ttcattaacc gtagatgttt tcttataaat ctcttttcta
      781 tctccgacat ttaaaatcag tatcagaatt ttatcatcct gaatatcggc aagcaggcga
      841 tagcaaccga tgcgatagct ccacttaccg ctatgagcag cagttaggct tttaccgtgc
      901 tgtcttggat ttgaacatcc ttccagattt ttccgtatcc agcccataat catggctgct
      961 gtataagagt caattttttt aaggtcacga aaggcagctt gtgtaaatat cacctgataa
     1021 ttcaacgtag ttccaactcc ttctctactt catccagtgt ataagtgacc gggttggctt
     1081 tgtattcagc taatgtcgct tcatagactt tgagatcaaa ctcatcctct atacgctcaa
     1141 ttacagattg gcgtaccagc tccgaaatac tcataccttt catttctgca tactttctta
     1201 taagttgggc atccgtatcg ttcaatctta tagatatagt catatcagtt cttcctttct
     1261 gtaatacatt gtattacggc atatttcagt tgtcaatata tagcaaaata tgtcaaatat
     1321 tgtgattgac taatgaatat aaatttagta aaattagtgt gcagaccaat cggtctacaa
     1381 aaagcggtgt accctaccat gagtgggaat caaaaaagcg gtgtacccta ccatgagtgg
     1441 gaattaaaaa agcggtgtac cctaccataa gtgggagcta aaaaagtaaa ggctgggtta
     1501 ccataattca gccttatctt tttagaggat ttttttatga gacttgagtt ttatactgtg
     1561 gatgcgaaat attgtgatta tttgagaaaa tttgattctt gtgttcgata gtacaggggt
     1621 ttttctttta aaagtgtatt aaagcaagag ttattgaata aaatgtagta tattacaaaa
     1681 agtcgttgaa gaaagtgttg cattagcaca aaaagtcgtt gaactttttg taaattgctg
     1741 atattatgtc tattgtgagc gaaaggaggt cgggtatgta tcgtacttct ttagaatatt
     1801 tgtataaatg gaaagaaaaa gacaatagaa agccgttaat tattcgcgga gcaagacagg
     1861 tgggaaagac ttggcttatg aaggaatttg cgaataaagc atataaatcg catgcatata
     1921 ttaattttga taacaatata caaataaagc agctgttttt gcaggattta aatgtagata
     1981 ggttaataat gggactggaa ctgtataccg gaaagaagat agttccgtcc gagaccctta
     2041 tcatatttga tgaagttcag gaagttcccg atgcactgac ttctttaaaa tattttaatg
     2101 aagatgctcc ttcatatcat attgtatgcg caggaagttt attaggtatt gcactgcatc
     2161 ccggaacatc ttttccggtg ggtaaagtcg agtttatgga tctttatcct ttgtcatttt
     2221 cagaatttat gctggcaatg ggaaaagaac agtatgccga actgttggaa tcaaaagact
     2281 atgagatgat tacaactttt aagcaggaat acattaatct tttgaaacaa tatttctttg
     2341 tgggcggtat gcctgaggtt gttgcacact acgttgataa taaggatttt caaggggttc
     2401 gggaaataca gagacgtatt ttagatgcat acgagcagga cttttccaag catgctccca
     2461 atgagattgt accccgcatt cgcatgcttt ggaacagtat tccggctcaa cttgcaaagg
     2521 agaataaaaa gtttatatac ggtctgatca aagaaggtgc cagagcaaaa gaatatgaaa
     2581 cggcactatt gtggcttacc gattgcggcc ttatccatca ggtgaatcgt gttaatgcgc
     2641 ctaatatacc ccttaaggca tatgaggatt taaaggcttt taagttattt accttggatg
     2701 taggtctctt atcctgtatg gtgggtctgc atcagagtgc tttgttaagc ggtaatgact
     2761 tgttcaggga attcaaagga gctttaacgg agcagtatgt tctgcagcaa ttaaaaacat
     2821 tggataattt gaatgtctat tattggacca atgacagaaa ccgagccgag attgattttc
     2881 tgattggcag tgagggtagg gttattccgg ttgaagtaaa agccgaagtt aatctgcaat
     2941 ctaaaagtct taaaacattt cgtgataaat atgatataga gatttcggtc aggacatcca
     3001 tgtcggacta taaaaaagaa gagcggctta tcaatttgcc gttatacgca ataagtaata
     3061 tcaaaaagat ttgaaaccgt atagcggagg atatgtgaag atgatataat ctaacaagat
     3121 gtagatttgt ccggataagt ttgaataaac aaataaatag aatgggaggt tgttatggga
     3181 agagatttga gaaaactgac tttgttgcat tcaaatgata tgcacggaga ttttatgagt
     3241 gagaatattg acgataaact tgtgggcgga gttgcaatgc tgtcagggta tatcgataaa
     3301 gtacgacagg aagaagaaaa tgtggtttat gttatagcag gagatatgtt cagaggatct
     3361 attattgatc aggaatacag aggactttcc acaatagaaa tagttaatat gctttcaccc
     3421 gatgtggtta cattgggaaa tcatgaagtg gattacggtg ttgcccatct tctgtttatt
     3481 gagaaactgg ccaagtttcc tattataaac agcaatttat atataaaaac caatcatacc
     3541 cgtcttttta gaccatatct cataaaggaa gtaggcggga taaaaatact gtatatcggt
     3601 atattgacgg aggaagttat tgctcagacc aaacaggaga ctctcatagg ttccatagtg
     3661 gatgtttatg aagccgcaaa ggaagtggga agaatttgta atgcctattc tcaaatagat
     3721 attgatttca caatacttct tacacatatc ggttttgaag aggataagaa attagcttcc
     3781 atgctggatc cggcatgggg agtggatatt attatcggtg gacattctca tacatatctg
     3841 gagcaacctg agatagtaaa cggtatagtt attgcacaag ccgggacagg taccgatcag
     3901 ataggaagat ttgatattat tgtcgataaa gacaacaact gcatagaaga ttttacctgg
     3961 caggcggtac ccatagatga gacgcattgc cctgtagata aagatttgga agaaataata
     4021 catcaatatc atgaaaagac cagtgaaaaa ttctccagaa taataaccag attcaagcgt
     4081 gcattaaccc atccgaaaag aagtcaggaa accgagttgg gcaatttatg tgcggatgcg
     4141 gtagtggata acctcggctt ggatataatg ttgtttgcat cgggaagcat aaggaaaaag
     4201 gaattgggtc ctatactgac acttaaggat tttaaagaat gtttcccgtt tgatgacaag
     4261 atatacagaa taaagttgac aaagaagcag atagaaaaag cattcaatac aattttggga
     4321 agagatttaa gcgaccatgt ggaattctat caggtatcca gagggttcaa ggtaaagtat
     4381 tccaagaaag agaacagagt taccgaaata tcattggaat cacagccttt ggatgataat
     4441 catatatact cggttggttt acaggagtac cattttaaca atatggcgga ctttataggc
     4501 atcaccaagg aagaagcgga agccaattcc cctataaaag tagttaccac ctccagcgga
     4561 gatgtgctgg aagaagcatt tttaggaatg gtgcttgccg atccgaaagt tgagggccgt
     4621 atagttatcg agtattagtt gaatagtaaa aacagattta tttgttgaat taaaccgttt
     4681 cggctatata tcataccgaa acggtttttt caggcagttc aaatactttg cttaaggtga
     4741 acttatgtaa acttaagcat tgtaagtttt attcgtattc atcaatctgt ttatttattg
     4801 atgcaagctc ttcttctaaa aacttcttct gattaagcag aagttccttt gagggctttt
     4861 catcatatag aggagcatca tcatagtaat acagcctgtt gcatacgcca aatcctcttc
     4921 ctctgcctcc gcctctgcca tagtatctgc ctaccggacg tctggcgcct gtgcagtatc
     4981 ccatagctct tcctgttagc gggcccattc ctctgggtcc tgttccgtca aatctgggca
     5041 tcttttgaac ctcccttatt tatttccggc atatgccgga tactatcagt ataccacatt
     5101 aatggcatat gtcaataact attttaatat ttaaccttgt ttcgttgaaa gtaatgtagc
     5161 agaggaatag ggtagtacaa tttcatgata taatacatat aacagaatct tgaacaagga
     5221 gtagcatatg tctcttacat caaaagaaag attaatgagg atttttgcca ataaaccgat
     5281 agacagaccc gcgataaagt tgtggggatc atggaattac cgtaagggtg ataaattatt
     5341 gcatccttca tatgagccgg caatgataaa ggccgcggaa aagaccgatc ttgttgttaa
     5401 tgcacactct ccgtttttca tgttctgcgg acaggtggat atagtcagga gaaagaatgt
     5461 ggagataaat gatcctctgt ggtacgagga acatatagtt ttccatacgc ctatgggcca
     5521 acttcgcagg gtagacagaa tatcaaaaat aggtgaaccg gggtttacga tagaatatcc
     5581 cgtaaaacaa ccggaggata taaagaggat gctgtctatg ccgtatgagc cgtttccgta
     5641 tgttgataat attgaagagc agcaacaact gttgggagac aggggaattg tcctgtaccg
     5701 tattgaccac atcgcatatg ccctttatcg tatgataggt tcggaaaatc ttgcatattt
     5761 tacccatgat tgcagagacc ttttaatgga agcattggat gttttttcca aaaggctgta
     5821 tgctcatgtc gaagaagcgc ttaatcatgg tgtcagagta cctttttcct gggtgggacc
     5881 ggagttgctt attcctccgt tattatcgga aaaagatttc atggatttct gttttgatta
     5941 tgataaacac atttgcgata tggtacacaa tgccggaagt tacatctggg tgcattgcca
     6001 cggtaaagta cggaacctga tagagaaatt tattgagatg ggtgtcgatg tgcttaatcc
     6061 tttggaaccg cccaagaacg gagatgtgga tttggacgaa gtggcaagaa cattcaagaa
     6121 tcgcataggt ctggaaggca atatagagat gcaggatttt tatgatgaat ccgaggaagt
     6181 tataagggaa aaaataaaaa cttgcgtgca aagcggaaag caaagcaaca gatttataat
     6241 gtgcacttcc gccggcttca tggattttcc gacaccaagc aagcaatatc ttgataatat
     6301 catgacatac atagattacg gatatgaatg cgtcaataat gcaaggtact agtaaatatt
     6361 attaacaatt atcttgatta aaagcattaa cctatactga ataccgcttt aatcttattt
     6421 gtatcgggct ttataacggc tttttgttcg gtaataactg ccgagcctcc cgatatgctt
     6481 tgtacggtta cttccaagtt tctgtttggt agccgtaaat gtaagcatag ttctctgtta
     6541 atatcaaaag aactcaaatc ggcagtgaag gttaccgtat tcttagatac ttctatattg
     6601 aaagatattt taccttggaa tgccggtgct tccttaactt ccattacatt gccttcttta
     6661 tagaaatatc tcggaaccgc acacgttaag tgcaacatat catctttaaa cagataatcc
     6721 gatacaagca tatcacgaat aaactgagtg acacatatag gtgtccatgc atgttgcaga
     6781 tctcctgtta ttctttttgc tcctttttct tcggttcgct cctcgcacca ggtaaacaga
     6841 ggagtgccgt gattgagtgc cgcaacaagg taatcggccg atatgtccgc tttatcggct
     6901 aatatgttta catatgccat ggcatccaat gcaatagcca cccataaacc gtcatgtatc
     6961 catccgaggt ttttcggcaa ccctccctca cttatattcc cctgtaactt attcatggta
     7021 cctacagcta agggatggtc tacgggaagt atattggtcg gataaaccgc atctgccaca
     7081 gcccatctgc tgcctgaagt attatgggca cagcttccta tccagatcga accgtccggc
     7141 tctttgatat gccctatttc cagtgacttt aatatacatt ctttgtgatc cagataagtg
     7201 tcaagcaact cttgataaac atcctctctt tgtaactctt tagccgccca aacagccgct
     7261 tccaatccca tacagtgcat gaagttgtga ggataaaaaa ctccgaaaag gtcatcgccg
     7321 tttttcagac cgccgtctcc catgcctctg ggcattaagc cccattcggg tgaatcggga
     7381 gggtttttcc gtttggtttt atctctttgt gccttggacc atctggcgga ggcaagcatt
     7441 ctttcaaaat tctcctctaa aaatgctctg tcttttgtcc gtaaatagta gtgttggata
     7501 cagcggcttt tccaacctga tgaagcccac atgtatttgc cccattgctt atcatcttcc
     7561 caggagccgt tattcagctg gaaacttgcc acaaactcga tgttttcctt tgcaggctca
     7621 aaaaatccgt atttacccaa agtaagtgcc tggaaacacg gctctccggt attggaactt
     7681 ctgtacattt ccgttccggc aagtcccgcc attctgccgt cagccatttc ttctctcatt
     7741 acgaatatat cgcacaggca cgctttatat gcattctcaa tcatggggtc gggtaaatca
     7801 aataccggtg cttttttgat aatattcctc cactcattca gccccagttc gtacagggca
     7861 ttccagtttg tcttcaacat aacttcaata tcgtcgttgt attttttatt ggggcgtata
     7921 agcatactct cacgggtttc ttttgctttg agtatatatg catatgtgac cgactctctt
     7981 ctggaaggaa cagtatcggt aagacttgca tccataataa ctatcctgtc cgaagcgtca
     8041 ttgaatatgg gatttaccac gctgtattcg cagtcaaaat ccgcccactt ggtattaatt
     8101 gtgttgtcca atacgcatgc gtcaataaat atttttttat cgctgccggt tgtattggta
     8161 acggacattt taataacatc tccgagtctt gtagccacac attgaacggt aaggtttgca
     8221 ccttccgcat ttgctttata caacagtgac ggaatatatc cctcaattct ctcccaggca
     8281 aaagcttcca tggcaacatc gttacacttt gccgttatct ttactttcca gtccagacga
     8341 actcttttgt atgcacccaa aggattatgt gttgtgtcgc tgtcgctcca cagcatggtt
     8401 aaagaatctt ttgtacagtc atacagtgtc ttgttactcg agttgggaag acatacacat
     8461 attctgtgag gttccgaaaa tgcgtacccg aaatcaattt ttgtctgctt atttcttttc
     8521 ataatttgta gctcctgctc ctttcaaatt taactcaatc ccagtattct tgcggcatta
     8581 ccgcctaata tcaaatcttt agaggtttga ttaatcggta agctgttaac ggcacctatt
     8641 tcatttttag cattactcca gggcgagtcg gaagcgaaca gtattctgtc tgccccgtga
     8701 gccttcacta ttcgcaagaa ttgttccttg ctgtaatatt caaagcccat tgaagtgtct
     8761 aaatatatgt ttttacctac aagatatctt tccacatcat cccacatgtt gtggctgcca
     8821 agatgagctg ccaccattac tcctccctgc atgtggtcgg caatatatgc gaatttttgc
     8881 ggattacttc tgtacggggg aggcataccg tgatcttcac cggcatgatg tacaattatc
     8941 aggcctttac tcaaagcata atcatatatc cttaaggctt tcggatcatc cacaacgaag
     9001 gcttgatatt ccacatgaaa ttttatacct ttcaagccta aaccgcagac gaaatctata
     9061 tcttttttgt aatcctccgt atgaggaaag attcctccga aagatattat tctatcatca
     9121 catacactct gtgcccaaag attggtgttt tgcagttgtg tgcttttggt tattaccgtt
     9181 tgaaccaccg atatatcaat accccattga tccatggttt tcataagccc tgcctttgta
     9241 agatcggtag tgggctcata tctgtagttt acatttctca aaagcgtctc cctggctttt
     9301 tttgcaagag tatcggggaa tatatgagtg tggaaatcta ttatcatgtc gttttttcct
     9361 tttcattaac tgtaacattt taataccttt tattcaagaa gcttcgggaa tatatttaca
     9421 atgcacaact ctcgccgaaa gttggagaag aataaaactg ttagacttta tttgattagg
     9481 gttttattgt aaaatcaact gtataaaaag tgtctgaaga aaaaaacagg ctgattataa
     9541 agttcatgaa actgtggagg aaaccgatat atgaagcccg aacttcttaa gttaagagaa
     9601 tatgcaaaac aatatgccga aatagcattg agcgacagaa atctggcaat accggagcaa
     9661 tacagaaaac ttaacagcct gcaacaggtg cgacctcccg ttcttgtttt tgaagtgccg
     9721 tggggtgaac tggaagatca ggaaaaactt cggttggaat gtgaaaccga cctgtacaga
     9781 ggaattgaaa gagccgttaa gagagctctt taccagtttg agtatttccg gggcgattat
     9841 gcaatacatc cgtattacaa ggtaaggata agaacaaaag gtaccggaat aggtcttacc
     9901 gtgcaggagg ttatgattgg ctccgccacg gggaccgata tcagtgctca tgagtatcat
     9961 gatattttac ccgatgagga atctttggaa aaaataacta tgcctacgat gcaggtggat
    10021 gaacaggcga cgcaaaaagc actgcaagcg gctcatgatg tatttgacgg cataatggat
    10081 gtaggacttg gcggctatca gctcatattt aatatgtggg atgaaatacc ccgatatcac
    10141 ggtgtggaga attgtcttat cgatatatat gaaagaccgg attttattca taagatgatg
    10201 gataagttta cacggtatta tgaggaaacc gtcagtctat atgaaaaact gaatatattg
    10261 gaaaccgatg catattatct tcactgtaca ccggcatgca cctatgaact gccggtaaag
    10321 gatgtgaccg aagaaaccgt cagtgccaag gatgtctggg gaaggggaat ggcgcagatt
    10381 tttgccgttg tatctcccga tatgcacaaa gaatttgaca taaagtacat gaagcggctg
    10441 tttgacaggt gcggacttac ttattacggc tgctgcgagc ctttacataa caaaattgat
    10501 attttaacgg agtttgataa tttgcggaga atatccgtaa ccccctgggc ggatgtcgac
    10561 attgcagcca atgccatggg aaacagatat gtgctttctg caaaatccaa tcccgcatat
    10621 gtgtcgcaag cagtatttga tcctcaaccg gttattgagg agacaaagaa aatacttctt
    10681 gcatgtaaga gaaacaacac gccttgcgaa tttgtattga aagatatttc cacggttaac
    10741 agaaatgtta atcatctgtc ggaatgggtt aagacggtat cgca
//
LOCUS       DAOPWC010000006        10765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_6202_length_10765_cov_4.778151, whole genome shotgun sequence.
ACCESSION   DAOPWC010000006 DAOPWC010000000
VERSION     DAOPWC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6202_length_10765_cov_4.778151"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PKJ65_00295"
     CDS             complement(<1..1875)
                     /locus_tag="PKJ65_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40306.1"
                     /translation="MRIIAVHIYGYGKLTDRTFNFKEGFNLVLGKNESGKSTLMSFIK
                     AMLYGHKKNEREKDGFIPEVKKYKPWNSDKYGGYLILETDEKKILRVERDFNGKTLSV
                     YNEYGEEITNEFSFSKENEMIGQDLLGMDYECFINSSFMCQDKNLLYAEDKEHIAQKL
                     MNINESGEEQISVGKAVKKLKEGITLLGNERTKGRKYNILSAQIREEEIKLTNMQNEN
                     KKCMEYLAEAEEIKKELEELENEEKLCKKSETFNRVNENIEEYERYNSEIEQLKKKLS
                     DTDIDAYEKRRKEFDTVAEQYKDAEKIYERLSEKEKRNNAKRTAFAILAILIIATGAV
                     LAITTDILLLLCASAAIPFIVLFLIMRKKNKELIIQAEKLENMIKTKNELVQIETVLG
                     NDKSVNGLIERYTGLQEDILRKEDVLDYSALLEKADFHNPQGKEPKWNYEECLTLIRE
                     KRERLAVVKAQLKEYMNSDEQTASQEERIELLKEQMKAVEEKQKALQYAIDGIEQASK
                     QIREEIIPKMNRKMSEYLKMITAGNHDNLSTGSTMDLNTEHNKTIRSVYNFSDGTLRQ
                     MYLAFRLAAAKVFSSECVTPVFMDETLVYYDEERKRSTFDFLYELSRHNQILFFATDM
                     E"
     gene            complement(1872..2918)
                     /locus_tag="PKJ65_00300"
     CDS             complement(1872..2918)
                     /locus_tag="PKJ65_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ40307.1"
                     /translation="MALTFFHMADIHLDQPFSSLGLQEGFPSKRREEIFHEFMRILET
                     AREEQPSFIFISGDLYEHEYTNIKRISRINEAFSELKGNIILISGNHDPESKNSFYRS
                     FPWSENVHVLGRSKPNVVFEKEKVCVFGIGYDTGGGQAKILETIRTDRKYINVLLLHG
                     DVDLSLSTYNAVSSDMLKDIGFDYVALGHNHRMYVKDNIYNPGSLCALKFGEDGKHGY
                     FKGDLSTGQVEFVESKARKYIDLKMDYEKFADFERMYPDRQDIYRIKVTGTKSDYQPL
                     ATPEGYDYVQLTDARTAEKKEKKQHVSQGIKGRFTDIMTTKMEEVLSEEIRIYEDALK
                     LGLQALSDEELDLE"
     gene            complement(2920..3300)
                     /gene="acpS"
                     /locus_tag="PKJ65_00305"
     CDS             complement(2920..3300)
                     /gene="acpS"
                     /locus_tag="PKJ65_00305"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNZ40308.1"
                     /translation="MRIACGTDIVENRRISDAVSRTDRFLEKNFTQREIEYCKSKKAL
                     MNQSLAARFAAKEAVSKALGTGFCAVVQPIHIEVINDDKGKPHIVLNEQLKTRFPEIA
                     DISVSLSHCKDYSVAYAVITFKEE"
     gene            complement(3297..4151)
                     /gene="ispE"
                     /locus_tag="PKJ65_00310"
     CDS             complement(3297..4151)
                     /gene="ispE"
                     /locus_tag="PKJ65_00310"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNZ40309.1"
                     /translation="MIVQKANAKINIGLDIKGLRQDRYHEIDTVMQTVELGDRITFAE
                     REKGIQFWCDDSSIKNNENTAFIAAKLFMKEMGIKRGIKIDLYKTIPLFSGLGGGSAD
                     AAAVLRGLNFLFNTGLPYEDLEKMAALIGSDVPFCIRGGTQRATGRGETLKRLKDFSD
                     VWFVIVKPKDSVETAWAYKEYDKISNPVRPDMDLIESLLEERDIEGLKGNVVNIFEKV
                     VIPHKPSIQKAIEDLSETDAVTVFMTGSGSGVAAMYNEMRLASMAYTSLSRKHDDIFI
                     TKTSGGLK"
     gene            complement(4155..5186)
                     /gene="tdh"
                     /locus_tag="PKJ65_00315"
     CDS             complement(4155..5186)
                     /gene="tdh"
                     /locus_tag="PKJ65_00315"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNZ40310.1"
                     /translation="MKAIIKAAAGMGNVELVQRPVPEINENEVLIKIKKAGICGTDYH
                     IYKWDKWSQNRIKPPLIIGHEFAGEIVEIGKSVKGFHIGQRVSAEGHITCQRCKLCRT
                     GRGHICEDVDIIGVDIDGCFAEYIKMPAINVWPLDDEVADKYGAIMDPLGNAMHTVMA
                     QPISSRDVLITGGGSIGLFATAIARKCGAARLVVSEPQPYKAALAKKAGADVVINPLS
                     DNPEQQVIEAFNGNRPEVVLEMSGFPSAFQLGLEVIENGGDMAILGIPSEELNINMGK
                     YVIFKGITLRGINGRRMFDTWYQCQNFLKSNSSFIDHVITHELPMEDVNEGFRLMSES
                     KAVKVVLDF"
     gene            5371..5811
                     /locus_tag="PKJ65_00320"
     CDS             5371..5811
                     /locus_tag="PKJ65_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ40311.1"
                     /translation="MKNLFDKLMQNRLFRELFLLFKYGIVGIINTGVTTGIFFLLSRL
                     GLKYYAYTVIGYVIGILTSFFLNRKYTFKKDNENVTRQIVLFFVISACLLGIVQLIQY
                     LLIDLLSLKEWLGVGIGMVFYTVTGFLLNRNVVFKYKDKENSGQ"
     gene            complement(5852..7165)
                     /locus_tag="PKJ65_00325"
     CDS             complement(5852..7165)
                     /locus_tag="PKJ65_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ40312.1"
                     /translation="MQLKSKLSIFLLVIIVVASMFAYMGAILVSNKNFIETIKENQRY
                     VAETIISANKETDLPLERIIAITHNALYPVKLTKDIKDLRLSNTEEALLENGIIVFIK
                     QRVIPVGISVVKTEEYYLRVGYQPENNLFRNYAMLLAYAFLFSIIVFMLIIMGLSNKA
                     MKPIVQLNNATQEVARGNFDVVVVNDSQDEIGQLTESFNKMAKELKSIEYLRKDFVSN
                     VSHEFKTPISSILGFAKLLENRNLTDEEKKEYLDIIVEESKRLSNLSTNILNLSKLES
                     QEIVFNKSEYDLDEQMRNVILLLEPKWYEKNISFDIEMENISLHANEDLMQQVWINLL
                     NNAITYSHPKGVIHIKVYNEEEQAVIVIRDEGIGMDEYTKERMFEKFFKGNSSLSKEG
                     TGLGLALVKRIIDLHHGTIEVESRPGEGTRIKLAIPNEFRGWERL"
     gene            complement(7149..7835)
                     /locus_tag="PKJ65_00330"
     CDS             complement(7149..7835)
                     /locus_tag="PKJ65_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ40313.1"
                     /translation="MFNILIVEDDKNLRKLIGTVLKQNGFNVLCANDGEQALEILDST
                     KADLIICDIMMPNMDGYELTESLRWSNYNMPILMITAKETLEDKRKGFLSGADDYMVK
                     PINTDEMLLRVNALLRRSKIATEHRIEVGEVVIDYDTLSVQSATERIILPKKEFLLLF
                     KLMSYPKKIFTRQQLMDDIWGLDSETDERTVDVHIKRLREKLVNIEEFEIVTVRGLGY
                     KAEKKNATEV"
     gene            complement(7931..8611)
                     /locus_tag="PKJ65_00335"
     CDS             complement(7931..8611)
                     /locus_tag="PKJ65_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNZ40314.1"
                     /translation="MSSIFYMIIITKREFEEAYLNFFRENDVHMVLSDLCRGTAQKKT
                     LDYLGIEKTEKIMFHIFVDSNRREKILRNLVNQMEIEVAGNGIALTIPLESIGGESCL
                     KYLTEGQEIGKKKGEKMENLKYSLIIVVADRGASETVMDAARSANAKGGTVVSAKETE
                     VNAKERFFGVSIAEEKEMIYILAKKQEKDEIMSAIMTKAGIGTDANAAVFSVPVDRIA
                     GLRSLMTD"
     gene            complement(8625..10172)
                     /locus_tag="PKJ65_00340"
     CDS             complement(8625..10172)
                     /locus_tag="PKJ65_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNZ40315.1"
                     /translation="MSRENREFIKHNRTILLEKLKESLSSVLPVTAVVFILCFIFFPA
                     DSGIMLTFIIGAVMLILGMGLFTLGADIAMMPIGEHVGSSITKSRKLWLIIIASLIIG
                     TAVTVSEPSLTVLAQQVPGIPDMVLILTVSLGVGLFLTFSMLRVIFRIKLAYVLMGVY
                     AVIFTLSFFAPKEFLSLAFDSGGASTGAMTVPFLLAFGAGAAAVRSDKDAHSDSFGFV
                     GLSAAGPILAVLLLGILYKPGNTTYEATAPLNPVKTTQITRLFITEIPGKLGEVALAI
                     LPIVVCFLIFQLAVLKLKKGQVIRIVVGLVYAYVGLVLFLTGANVGFLQMGSFLGKAL
                     GSLPYNWIVIPIGMVIGYFLVISEPAVHVLSKQVVELSSGAIPKKAIATSLSVGVATS
                     IGLCMIKILTSASILYFVIPGYVIGLILTFFVPPVFTSIAIDSGAVSSGPMASTFLVP
                     FAIGVCLSVGGNIIVDAFGIIALVTMTPFITIQVLGLFYRFKLKAAEKEQEIQQKLKK
                     KESIINF"
     gene            complement(10272..10583)
                     /locus_tag="PKJ65_00345"
     CDS             complement(10272..10583)
                     /locus_tag="PKJ65_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HNZ40316.1"
                     /translation="MDSRKFTMKDESFVCEACGAKVHALGVTSRNHCPRCLYSKHVDN
                     NPGDRMCDCHGLMQPIGLEKYRDSYKIVFKCQRCGAVKKNIVAKDDNTDKIIELSSKP
                     V"
BASE COUNT         2811 a         2489 c         1776 g         3689 t
ORIGIN      
        1 ttccatatcg gttgcgaaaa acagtatctg attatgtctg cttagctcgt acaggaaatc
       61 aaaagtactc ctctttcttt cttcatcata atatacaagt gtctcatcca taaaaacagg
      121 ggttacgcat tcggaagaaa aaacctttgc agcagccagc cggaaagcca gatacatctg
      181 ccttaacgtt ccgtcggaga aattgtatac ggagcgtatt gttttattat gttcggtatt
      241 taggtccatt gtgctgccgg ttgataagtt gtcatgattt cctgcggtta tcattttaag
      301 atactcgctc atttttcggt tcatcttggg aatgatttct tctcttatct gcttggaagc
      361 ctgctctatg ccgtctattg catattggag tgctttctgt ttttcctcta ccgctttcat
      421 ttgctctttc agcagttcga tacgctcttc ctgtgaagct gtctgctcgt cggagttcat
      481 atattcttta agttgagctt taaccaccgc caatctttct cttttttccc gaataagggt
      541 taagcattct tcataattcc atttcggttc cttgccttgc gggttatgaa aatcggcttt
      601 ttccaataga gcactataat ccagtacatc ctctttgcgt aaaatatctt cctgtaatcc
      661 cgtatatctt tctatcagac cattaacgga tttgtcgttt cccaatactg tttcaatttg
      721 caccaactcg ttctttgttt ttatcatatt ctccagtttt tccgcttgga taatcaattc
      781 tttattcttc ttgcgcatga taagaaacag cacgataaac ggtattgcgg ctgaagcaca
      841 caacagcaac aatatatccg ttgttattgc caacacggcg cctgttgcaa taatcagtat
      901 cgcaagtatc gcaaaagcgg ttctttttgc attgtttctt ttttcttttt ccgaaagtct
      961 ttcatatatt ttttccgcat ctttatattg ctcggcaacg gtatcaaatt cttttcttct
     1021 cttttcataa gcatctatat cggtatcgga aagtttcttt ttcagctgtt ctatttcgct
     1081 attgtatctt tcatattcct caatgttttc attgacgcgg ttaaaagtct cacttttctt
     1141 gcagagtttt tcctcattct ccagttcttc aagttctttt tttatctctt ccgcttcagc
     1201 cagatactcc atgcattttt tattctcatt ttgcatgttt gtaagtttaa tttcctcttc
     1261 ccttatctgt gcactcaata tgttatattt tcttcctttt gttctttcat tacccaataa
     1321 agtgatgcct tctttaagtt tctttacagc ttttcctacc gaaatctgtt cttcccccga
     1381 ttcgtttata ttcataagtt tctgagcaat atgctcttta tcttctgcgt acaaaaggtt
     1441 tttgtcctga cacatgaatg atgaattgat aaagcactca tagtccatgc ccagaagatc
     1501 ctgtccgatc atctcgtttt ctttgctgaa ggaaaattca tttgttatct cttcaccgta
     1561 ttcattgtat acggacagag tttttccgtt gaagtctctt tctactctta atattttctt
     1621 ttcatcagtc tccagtataa gatatccgcc atatttgtct gaattccacg gtttgtactt
     1681 tttgacttcc ggaataaaac cgtctttttc tctttcgttc tttttatgcc cgtaaagcat
     1741 cgcttttata aacgacatca gagtgctttt accggactcg ttttttccca acactaaatt
     1801 aaagccttct ttaaaattga atgtcctgtc cgtcagttta ccgtatccgt atatgtgaac
     1861 cgctataatc ctcattccaa atcaagctcc tcatccgata aagcctgcaa tcccagtttt
     1921 aaagcatctt catatatcct tatctcttcc gataaaacct cttccatctt agttgtcatt
     1981 atatcggtaa atcttccttt tattccctgt gatacatgct gctttttttc ctttttctcc
     2041 gcagtccttg catctgtcag ttgtacatag tcatatcctt caggcgttgc aagcggttga
     2101 taatcgcttt ttgttccggt tacttttatc ctgtaaatat cctgcctgtc aggatacatt
     2161 ctttcaaagt ccgcaaattt ttcataatcc atctttaaat caatgtattt tcttgctttg
     2221 ctttctacaa attccacttg tcccgtagat aaatctcctt taaaatatcc atgctttccg
     2281 tcttcaccga atttcaacgc acaaagactg ccgggattat atatattgtc ttttacatac
     2341 atcctgtggt tgtgtcccaa tgccacataa tcaaatccta tatctttcaa catatccgaa
     2401 ctgacggcat tgtatgtgga aagagacaaa tccacatcgc catgcaaaag cagcacatta
     2461 atatactttc tgtcggtacg aattgtctcc aaaatttttg cctgaccgcc tccggtatca
     2521 tagcctattc cgaagacaca tactttctct ttttcaaaaa caacattcgg cttgcttctt
     2581 cccaacacat gcacattttc cgaccacggg aaacttctgt aaaaagagtt cttggattcg
     2641 gggtcatgat tgccggatat aaggataata tttcctttaa gttcggaaaa ggcttcatta
     2701 atcctgctta tcctttttat gttggtatat tcgtgttcat aaagatcccc tgatataaat
     2761 ataaaagagg gttgctcttc ccttgccgtt tccagtatcc tcataaactc atggaaaatc
     2821 tcttctcttc tttttgaggg aaatccttcc tgcaatccta atgatgaaaa cggctggtcc
     2881 agatgtatgt ctgccatatg aaaaaatgtt agagccatct tactcctctt taaaggttat
     2941 taccgcatag gcaaccgaat agtccttgca atgtgagaga cttacactta tatccgcaat
     3001 ttcggggaag cgggttttaa gttgctcatt caatacaata tgcggttttc ctttatcgtc
     3061 atttataact tcaatatgaa tgggttgcac aacagcgcaa aatcccgttc ccaacgcttt
     3121 tgataccgct tcctttgctg caaatcttgc ggcaaggctt tgattcatta aggctttttt
     3181 ggatttgcag tattctatct ccctttgtgt aaagttcttt tccaaaaatc tgtccgtacg
     3241 actcacggca tcggatattc ttctattctc cactatatcc gtaccgcatg caatcctcat
     3301 ttaagtcctc ccgaggtctt ggttatgaat atgtcatcat gttttcttga caaagacgtg
     3361 tatgccatgc ttgcaagtct catttcgttg tacattgccg ctacccccga tcccgaaccg
     3421 gtcataaaca ccgtaacggc atcggtttcg gaaagatcct ctattgcctt ttgaatggaa
     3481 ggtttgtggg gtataacgac tttttcaaat atgtttacaa cattgccttt aagtccttca
     3541 atgtctcttt cttccaataa agattcaatc aggtccatat ccggtctcac gggattgctt
     3601 atcttatcat attctttata tgcccatgcc gtctccacgg agtctttggg ttttactata
     3661 acaaaccaaa catcggaaaa atctttcagc cgctttaatg tctcacctct tccggttgct
     3721 cgttgtgttc ctcctctgat gcaaaaaggc acatcggaac ctatcaatgc agccattttt
     3781 tccaaatctt catacggaag acctgtatta aaaaggaagt taagtcctct taataccgca
     3841 gcggcatcgg cgcttcctcc gcccagaccg gaaaacagag gtatggtttt atacaggtca
     3901 atttttatac cgcgttttat gcccatctct ttcatgaaca gctttgcagc aatgaaagcg
     3961 gtattttcat tgttcttaat agaactgtca tcacaccaga attgtatacc tttctctctc
     4021 tcggcaaaag ttatacggtc acccagttcc accgtttgca taacggtatc aatttcatga
     4081 tatctgtctt gccttaaccc tttgatgtcc agaccgatat ttatttttgc attcgccttc
     4141 tgtacaatca tcttctaaaa atccagtact acttttaccg ctttgctctc agacatcagt
     4201 ctgaatccct cattaacatc ttccatggga agttcatggg ttattacatg atctataaat
     4261 gaggagttgc tcttcagaaa attctgacac tggtaccagg tatcaaacat ccttcttccg
     4321 ttaattcctc tcaatgttat tcccttgaaa ataacatact tgcccatatt tatattcaat
     4381 tcttccgacg gaattcccaa tatggccata tctccgccgt tttctattac ctccagtccc
     4441 aattggaatg cagacggaaa accgctcatt tccaatacaa cttccggtcg gttaccgttg
     4501 aaagcttcta ttacctgttg ctcgggatta tccgataaag gatttataac cacatcggct
     4561 cccgccttct ttgcaagcgc cgctttgtac ggctgaggtt cgctgacaac cagtctggcc
     4621 gctccgcact ttcttgctat ggcagttgca aacaacccta tggaaccgcc accggttatg
     4681 agaacatctc ttgacgatat gggctgtgcc attaccgtat gcatggcatt acccaaagga
     4741 tccattattg ctccgtattt gtcggcaacc tcgtcgtcca agggccatac gtttattgca
     4801 ggcatcttta tatattccgc aaaacaaccg tctatgtcca ctccgattat atccacatct
     4861 tcgcatatat gacctcttcc ggttctgcaa agtttgcacc tctgacaggt aatatgtcct
     4921 tctgcagaca ccctctggcc tatatgaaac ccttttaccg attttcctat ctccactatc
     4981 tctccggcaa actcatggcc gattataagc gggggtttga ttctgttctg tgaccactta
     5041 tcccatttat atatgtgata atcggttccg catattcccg ctttttttat tttgataaga
     5101 acttcgtttt cgttgatttc gggaacaggt ctctgcacaa gttctacatt tcccattcct
     5161 gctgccgcct ttataattgc tttcataaat actccttaat aacagtttaa ttttatcata
     5221 tcccgctcta tttgttaatg tatttaacat gtcggttttc tgcacttccg taaagggccg
     5281 atatatctta gcgtctgaac ggttacgggt tttatctgta atatgctgta tttcactgaa
     5341 aatccgtatt ttttatagta taatgaccgt atgaaaaatt tattcgacaa gcttatgcaa
     5401 aacagacttt tccgagaatt atttctttta ttcaaatacg gcattgtcgg tattattaat
     5461 accggcgtaa ccacgggaat attcttttta ctgagccgtc tgggacttaa atattatgct
     5521 tacactgtca tcggatatgt aataggcata cttaccagct tctttttaaa cagaaaatat
     5581 actttcaaaa aagacaatga aaatgttacc cgacagattg ttctgttctt tgtaataagt
     5641 gcctgcctgt taggcatcgt gcagcttata cagtatctcc ttattgattt attatctctc
     5701 aaggagtggt tgggtgtggg tatcgggatg gttttctaca ccgttaccgg ttttctgctc
     5761 aatcggaatg ttgtttttaa gtataaggat aaggaaaact ccggacagta aaactctcag
     5821 actcctttta attaaatatt aatgtttgtt tctataatct ttcccagcct ctgaattcat
     5881 tgggaattgc cagctttatt cttgtcccct ctcccggtcg gctctccact tcgatagttc
     5941 cgtgatgaag gtcaataatt ctcttaacga gggccaggcc taatcccgtt ccttcttttg
     6001 acagcgagga gttgccttta aagaattttt caaacattct ttctttggta tattcgtcca
     6061 ttcctatgcc ttcgtcccgt ataactatta cggcctgttc ctcctcgttg tacaccttta
     6121 tatgtataac ccctttggga tgggaataag taatggcatt attcagaaga tttatccata
     6181 cctgctgcat taaatcttca tttgcatgaa gagatatgtt ttccatctct atatcaaaac
     6241 tgatgttttt ttcataccat ttaggttcca aaagtaatat aacatttcgc atctgttcat
     6301 caaggtcgta ttccgatttg ttgaatacta tctcctgact ttccagcttg gacaggttca
     6361 atatattggt ggaaagattg gaaagccttt tggattcctc gacaattata tccagatatt
     6421 cttttttctc ttcatcggtg agattccggt tttccaaaag ctttgcaaat cccagaatcg
     6481 acgatatggg cgtcttgaac tcatgggaaa cattgctcac aaaatccttt ctcagatact
     6541 cgatactttt aagttccttt gccattttat tgaaactttc cgtcagctgt cctatctcat
     6601 cctgagaatc atttaccaca acgacatcaa aattacccct ggccacttcc tgtgttgcat
     6661 tattaagttg cactatgggc ttcattgctt tattgcttaa tcccatgatt atcagcatga
     6721 acacaataat agagaacaga aacgcatacg caagcaacat ggcatagttt ctgaacaggt
     6781 tattttccgg ctggtatccc actcgtaagt aatattcctc ggttttcaca acggatatcc
     6841 ctaccggtat tactctttgc tttatgaaga caatgattcc gttttccagt aatgcttctt
     6901 ccgtgttgct caaacgaaga tcctttatgt ctttggtgag tttaacggga taaagggcat
     6961 tatgagttat ggctataatt ctttccaaag gaaggtcggt ttctttattg gccgatataa
     7021 tggtttcagc cacatacctc tggttttctt ttatagtttc tataaagttt ttattggaca
     7081 ccagaattgc tcccatatac gcaaacatgg aagctactac aatgatgacg agcaggaata
     7141 ttgatagttt agacttcagt tgcatttttc ttttccgctt tgtaacccag tccccttacg
     7201 gtgactattt caaattcttc tatattcaca agtttttctc tcagtctctt tatatggaca
     7261 tctacggttc tttcatccgt ttcactgtcc agtccccata tatcatccat cagttgctga
     7321 cgcgtaaaaa tcttttttgg gtaactcatt aatttaaaaa gcaaaagaaa ctctttttta
     7381 ggaagaatta ttctttcggt agcactctgt acgctcaatg tgtcataatc gataaccact
     7441 tccccgacct ctattctgtg ttcggtagct attttagacc tccttaaaag agcgttaacc
     7501 ctcagcagca tttcatccgt gttaataggt tttaccatat aatcatccgc accggacaga
     7561 aaccctttcc tcttgtcctc cagtgtttct ttggcggtta tcatgagaat gggcatattg
     7621 taattggacc accttaaact ttccgtcagt tcatatccgt ccatattggg catcattatg
     7681 tcacatatga taagatcggc ttttgtgctg tcaagaattt ccaaagcctg ttctccgtca
     7741 ttggcgcaga gtacattaaa accgttctgt ttaagaaccg tccctatgag ctttcttaag
     7801 tttttatcgt cttcgactat taatatatta aacataaaaa atctctccga tacatattat
     7861 atatcaacac cgccatgata tgaattgaat acactccgga aagagaaatt atttataaaa
     7921 tatccgttgt tcagtcggtc attaagctgc gtaatcccgc tattctgtct acgggaacgg
     7981 agaataccgc tgcattggca tcggtaccta tacccgcttt tgtcattatg gcactcatga
     8041 tttcatcttt ttcctgcttc tttgcaagga tgtatatcat ttctttttct tccgctatgg
     8101 aaacaccgaa aaatctttct tttgcattta cttcggtttc ttttgccgaa actacggtgc
     8161 cgccttttgc atttgcactg cgcgcagcat ccattacggt ttccgatgct cctctgtccg
     8221 ctacaacaat tattaatgag tattttaagt tttccatctt ctctcccttt tttttgccga
     8281 tttcctgtcc ctcagtcaga tactttaagc aagactctcc ccctatgctt tccaaaggta
     8341 ttgtaagcgc tataccgttg ccggcgactt caatttccat ctggtttaca agatttctta
     8401 atattttttc tctgcgatta ctatctacga aaatgtgaaa cattattttc tctgtttttt
     8461 ctattccgag ataatccaga gtcttctttt gcgcagttcc tctgcataaa tcggacaata
     8521 ccatatgtac atcgttttcc ctgaagaaat taaggtatgc ctcttcaaac tctctttttg
     8581 taattattat catataaaag atactgctca tttcacacct gccatcaaaa attgatgatg
     8641 ctttcctttt tctttaattt ctgttgtatc tcctgttctt tctccgcagc ctttaacttg
     8701 aaccggtaga acagtccgag aacttgtatc gttataaacg gcgtcattgt aaccaatgct
     8761 attattccga atgcatctac tattatattc ccgcctacac ttagacatac tcctattgca
     8821 aaaggtacca gaaaagttga ggccataggt ccggatgaca cggcgcccga gtctatagct
     8881 attgaagtaa agacgggcgg tacgaaaaat gtcaatatca acccgataac atatccgggt
     8941 ataacaaagt ataaaattga cgcacttgtt aaaatcttaa tcatgcaaag ccctatcgat
     9001 gtggctaccc cgactgaaag actggtggca attgctttct tgggtattgc tcccgatgac
     9061 aactccacaa cctgtttgct taatacgtga actgccggtt cggaaataac caggaagtat
     9121 cctattacca tgcctatagg aatgacaatc caattgtacg gcagactccc cagcgctttt
     9181 cctaaaaaac ttcccatctg tagaaaacct acattggctc cggtaaggaa aagaaccagc
     9241 ccaacatatg cgtatacaag acctactaca attctgataa cctgtccttt tttgagcttt
     9301 aaaaccgcca gttggaaaat gagaaaacat acaactatag gcaatattgc caaagccact
     9361 tcacccaatt tccccggtat ctccgttatg aacaatcggg ttatctgcgt tgttttgacg
     9421 ggattaagcg gagcggttgc ttcatatgtc gtgttgccgg gtttatataa aatacctaac
     9481 agcaatacgg ctaatatagg tccggccgca cttaatccta caaatccgaa actgtcgcta
     9541 tgtgcatctt tatcgcttct taccgcagct gcacccgcac cgaaagccaa aagaaacgga
     9601 acggtcatgg ctccggtgga agctcctccc gaatcaaaag caagcgaaag gaattcttta
     9661 ggagcaaaaa acgataatgt gaatattacc gcatacactc ccatcagaac atatgccagt
     9721 ttaattctga aaataactct taacatagag aaagttaaaa acaatcccac tccgagcgat
     9781 accgtcaata tcaaaaccat atccggtatt ccgggtacct gttgtgccaa tacggtaaga
     9841 ctgggttccg atacggttac cgcagttccg ataataaggc ttgcaataat aatcaaccat
     9901 aattttcgtg attttgttat tgacgaaccg acatgctctc cgataggcat catggctata
     9961 tcggcaccta atgtaaacag ccccattcct agaatcagca tcactgctcc gataatgaaa
    10021 gtcagcatta ttccgctgtc ggcagggaaa aatataaagc aaagtataaa aacaactgcc
    10081 gtaaccggta aaaccgaaga aagtgattcc ttcaattttt caagaagtat ggttctgttg
    10141 tgttttataa attccctgtt ttctctgctc attacatacc tttctaatca atggataata
    10201 atataccata atgtatggga atttgtaaat taattatgaa aaacaaagct tgttatacaa
    10261 gactaactac attaaacagg tttagaagat aactcgataa ttttgtccgt gttatcgtct
    10321 tttgcaacaa tattcttctt aacagctccg catctttgac acttgaatac tattttataa
    10381 ctatccctat acttctccag gcctataggt tgcatcaacc cgtgacaatc acacatacga
    10441 tctccgggat tgttgtccac atgtttggaa tacagacaac ggggacaatg atttcttgaa
    10501 gtaactccga gagcatgtac ctttgcgccg catgcttcac aaacaaatga ctcatctttc
    10561 attgtaaatt tccgggaatc catatatgct cccatattca agttcaaaaa tctccatcgc
    10621 ctttcctttc tgtctttcta agcgaaaaaa aggaaaaacc caagcgatat atcaaaaaga
    10681 cacctttcag tcgtgaaacg atgtccttta tggcggagag acagggattc gaaccctggt
    10741 tactattcct agtaaacacg atttc
//
LOCUS       DAOPWC010000007         9296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_7463_length_9296_cov_4.183855, whole genome shotgun sequence.
ACCESSION   DAOPWC010000007 DAOPWC010000000
VERSION     DAOPWC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_9296_cov_4.183855"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKJ65_00350"
     CDS             complement(<1..775)
                     /locus_tag="PKJ65_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ40317.1"
                     /translation="MLHIGTGYVDITDGFWKKRQDINKDVTVKAVQNRFLETGRFDAF
                     DFTNEKIAHIFYDSDVAKWIEGVSFILHKERNSELEVFIDRIVDLIEKNRSDDGYYNS
                     HFLRKPEERWQHRSDHELYCAGHLMEAAVQYYKATGKEKFLKLMEDYADYIYKVFITE
                     KSAAFKTPGHEEIELALVKMYDTTGKKKYLELSKHFIDRRGVEEEPWIGSNYNRYNTQ
                     DFLPVRQQRTAEGHAVRASYLYSGMADIAYRYKDKELLAA"
     gene            complement(820..3180)
                     /locus_tag="PKJ65_00355"
     CDS             complement(820..3180)
                     /locus_tag="PKJ65_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40318.1"
                     /translation="MKKNIVCIGILLLVLVFSVITLASFPTDDAVIVNTEERVQSEQA
                     TVQQPATEDVMKGPWENSVKIYGNTTHSLSFSDSFSEKWFELEPMTEGKSVILTISCD
                     DQAATGVISALYYKEDVLSQGNNAERLFGTYMLNKPSAYKISKGGSYYLKVSVIGNSA
                     IGSNIKLNYTTIVNDIYEDNDVWERATPIDIKKPYIIDISAINDIDWFKISLQKDQAL
                     QYIIRNDISSGKGVNVALYREEDLIKNGDTAPVLFTSTEFNKYYTHKNDKTQTYYLKV
                     TPSAREAVLNKVTLITDIIPPDSLEGNDSYDQAVMLTPGVAKTVTISASNDVDWLMVK
                     TDKPGQTVKLSIRNASFSGAKLICDWYEGARLIAGSEAAETLYKTRGTGTSYVYMEEA
                     GTYYFKLYTVDNSVITTPIEISYTVIPADRYEPNNDYQSAKTISGNSETRFTLPALND
                     QDWFTVKTYKENQALRLNTYIPKTGASVNIFVFRQDDVESYGEAAPLFTFNSAKAPYI
                     YMLEEKGTYYIKIVSDTIISDDCILYLSLLNSDANELNNTRDTATYFPAGERKTFTIS
                     GVNDTDWFYFNVGKDNSVIDYIMQSDFNKYAKVSIYSEAELDSYEEYAAPLFTSDKYD
                     ERVAYKLDKAGKYYIKIDSALPVLDDIHLEYTIRESDKHENNDTWNKATPLTPTEKID
                     FTIRAANDADWFKISSSGTNKMSLTFNISSKYMMKDAINIYVYREVDLLNYGSRAQHI
                     YVARTSDLTNLIDIADGTYYLKAVAVKNDAFDFEFTLRANVFKMLD"
     gene            3369..4079
                     /gene="fabG"
                     /locus_tag="PKJ65_00360"
     CDS             3369..4079
                     /gene="fabG"
                     /locus_tag="PKJ65_00360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNZ40319.1"
                     /translation="MRTVLITGASKGIGRQATEDFLAAGYNVIANFHTTKMDKSEYPS
                     FQEHIEVIKADVSDYGQVEEMFKKGQVRFGNIDVVINNAAVNYAGLLSRMEEKDWDRI
                     INVNLKGIFNVCKCAVPSMVYRKAGHIINISSMWGITGASCEVAYSVSKAGVIGFTKA
                     LAKELAPSNVYVNCVAPGIIQTDMNKAYTQQELEEMIPLGRIGKCRDVSEMLLFLADE
                     KTSFITGQVFSVNGGTVI"
     gene            4144..4536
                     /locus_tag="PKJ65_00365"
     CDS             4144..4536
                     /locus_tag="PKJ65_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40320.1"
                     /translation="MMRRLREFFMNRYGTDQLNRALMVVYALFFFVYLITKNNVFLFI
                     ALAVALYQVFRTLSRNFTQRAKENQFFLKYWNPLKGKYFSVKKKATDKTHRYYKCPKC
                     KTEVRVPKNKGKIRITCPKCSEQFIKKT"
     gene            4642..5553
                     /locus_tag="PKJ65_00370"
     CDS             4642..5553
                     /locus_tag="PKJ65_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ40321.1"
                     /translation="MEFLKTLEQLRTPFFDQFFLLVTKMGEELFFTVVILIVIWCIDK
                     KTGYRMFFIFLLGSFVNQMLKLIFKVPRPWTYENAAKPVEGSVEAATGYSFPSGHTQQ
                     GICLFGSVAMFLKKTRVYIVMVILTLLVAFSRMYLGVHTPADVFTSLVIGVVLLLLCY
                     YAFRVADKHKYGDEYLYGIVAFAIVISLIVLSFDKTGSPEYLHGIENGWKLLGCCIGI
                     IVSIIVDIKYTNFEVKAKWWQQIIKLVVGLGMVLVIKSFLKQPLLDLFNQSPVADGIR
                     YLLIILFAGCLYPMLFKIFKRNSKADS"
     gene            complement(5596..6690)
                     /locus_tag="PKJ65_00375"
     CDS             complement(5596..6690)
                     /locus_tag="PKJ65_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ40322.1"
                     /translation="MNATSSKIIRTIAILVIIVVIIGLGAWYIAEHGFSGLSFSFVEG
                     HRIEIRRGQFEEKARYETNADGIKEIQVKWITKAVEFKVHDHEKILIAESCQRDLAKG
                     ENLTYTVKDGILKISELENESIFKIAPKKVVIWIPKTLSESLSALKADCQITDITAQD
                     ITADIVYLETHTGRINVSNLNASRTVTLNTDTSSITASELKAKSVTVKSDTGNISLNR
                     ITAEEKINAETDTGRIEANDVITETLYCKSDVGRVICKNYKVKSITQKSDTGMVETEG
                     SFKTGEFRSNTGSITIKCAAPPDSIYADTDTGSIYLYIVKTDKLVVSYSTDVGKFDAE
                     IPVLVSHSADANINLKTSTGNIYIKPYEAE"
     gene            complement(6704..7327)
                     /locus_tag="PKJ65_00380"
     CDS             complement(6704..7327)
                     /locus_tag="PKJ65_00380"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HNZ40323.1"
                     /translation="MENKLKAYVNNLFRNAPATAKAYEVKEEILQNLKDKYNDLVTEG
                     KSKDEAYKTAIDSIGDLSELFAELEKEITPDDSKRQKYLALRSVVAMIFVLAAVPLVM
                     ALSYKSVLINVMIAVILVAIGIILAIYNRTNKPGYRHSNTQTDRMAEIERKRSIRKSV
                     SGFLWLITLIMYFIVSFQTNAWHLTWLIFLLAAAIETILDIWLGKAK"
     gene            complement(7317..7664)
                     /locus_tag="PKJ65_00385"
     CDS             complement(7317..7664)
                     /locus_tag="PKJ65_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ40324.1"
                     /translation="MTIASDLIRGNTDTIILASLMKKDSYGYEINKEISAKTAGEYEF
                     KEATLYTAFKRLEEAGFITSYWGEDNVTARRRYYSITERGKEEYINLLSDWNRSKNMI
                     EKLIDTGVMEDGE"
     gene            complement(8038..>9296)
                     /locus_tag="PKJ65_00390"
     CDS             complement(8038..>9296)
                     /locus_tag="PKJ65_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNZ40325.1"
                     /translation="IRKSPNEPEAKVRLVEKFEFTDRQAQAIVDMRLGRLTGLEILKL
                     QTEYDELTEKIKYYLSILEHEDITHRVVMEELEVIKKKYGDERRTAIEDSEEDLLDED
                     LIKEETVVLTLTRFGYIKRTPVDTYKSQNRGGKGIIGVTTREEDTIVDIFTTSTHSTV
                     LCFTDRGKVYKLRGFQIPEASRTARGTAIVNLLELDSNEKVTTILPIESFETTQNLFM
                     ATKLGFVKKTPLISYVNIRKSGIIAVTLREEDDLIAVRLSGGDDTVMLITNKGKAIKF
                     TEEDTKETGRSAKGVIGIRLDDDDYVVGMSLVKEEETILSVTENGFGKRTFVSEYPIQ
                     KRAGKGVINYKVTERTGTIIASMIASDQDDLLLMSLQGDMIRLDVGQISILSRATQGV
                     TLMRIEEDNAVVAATITEKEQEEAEE"
BASE COUNT         2386 a         1992 c         1753 g         3165 t
ORIGIN      
        1 atgcagcaag aagttccttg tctttgtatc tgtacgcaat atccgccatt cccgaataga
       61 gataagatgc tctgactgca tgtccttccg ccgtcctctg ttgccttaca ggcagaaaat
      121 cctgtgtgtt atatctgttg tagttgctgc ctatccaagg ctcttcttca acacctcttc
      181 ggtcaataaa gtgcttggaa agttcaagat acttcttttt tccggtcgtg tcgtacatct
      241 tgacaagagc aagttctatt tcctcatgac ccggtgtttt aaatgcggcg gatttttccg
      301 ttataaacac tttgtatatg tagtcggcat aatcttccat cagttttaaa aacttttctt
      361 ttcccgtcgc tttataatat tgcacagcgg cttccataag atgacctgca caataaagct
      421 catggtccga acggtgctgc catcgttctt ccggttttct taaaaaatgc gagttatagt
      481 aaccgtcatc cgacctgttc ttttcaatga gatctactat gcggtctatg aatacctcca
      541 actcgctgtt tctctcttta tgcagtatga aagatacacc ttctatccat ttggctacat
      601 cggaatcata gaagatgtgt gctatcttct catttgtaaa atcaaacgca tcaaacctgc
      661 ctgtttccag aaacctgttt tggacggctt ttaccgtaac atccttattt atatcctggc
      721 gcttcttcca gaaaccgtcg gttatatcta cataaccggt gcctatatgc agcatatcca
      781 gtcctccttt gcggtttgta tatcaatcag aaatatacat taatccagca ttttgaaaac
      841 atttgctctg agtgtaaact caaaatcaaa agcatcattt ttaaccgcta ctgctttgag
      901 gtagtaagta ccgtccgcaa tatcaataag attggttaaa tcggatgtcc tggcaacata
      961 aatatgctga gctcttgagc cgtaattcag aagatccacc tctctgtaca catagatgtt
     1021 tatagcatcc ttcatcatat actttgaact gatgttgaag gtcaggctca tcttattagt
     1081 gccgctgctg cttatcttga accagtcggc atcatttgca gctcttatgg tgaaatcaat
     1141 cttttcagtg ggggttaacg gtgtcgcttt attccatgtg tcgttattct catgcttatc
     1201 cgattctctg atggtatact ctaagtgtat atcatcaaga acgggtaaag cggaatctat
     1261 cttgatgtaa tattttcccg ccttgtccag cttatatgct accctctcgt catacttgtc
     1321 ggaggtaaac aacggagcgg catattcttc ataactgtcc aattccgctt cactgtatat
     1381 cgatacttta gcatatttgt tgaaatccga ttgcattatg taatctatta cactgttatc
     1441 ttttcccaca ttgaaataga accagtcggt atcgttaact cccgatattg taaaggtttt
     1501 tctttcaccg gccgggaaat aggttgcagt atctctggtg ttgttcagtt cgttggcatc
     1561 ggagttcagc aacgaaagat acaatataca gtcatcggaa ataattgtat cggatactat
     1621 tttaatgtaa taggtaccct tttcctccag catgtatatg tagggagcct tggcgctgtt
     1681 aaaggtgaaa agaggagcgg cttctccgta cgattccaca tcatcctgtc tgaaaacaaa
     1741 tatatttacc gatgctccgg tcttgggaat atatgtgtta agtctcaatg cctgattctc
     1801 tttgtatgtt ttaactgtaa accagtcctg atcattcaat gcgggaagcg tgaaacgtgt
     1861 ttcactgttg cccgatatgg tttttgcact ttgataatca ttgttgggtt catatcggtc
     1921 tgccggaatc acagtataac ttatctctat cggagtagtt attacgctgt tatcgacagt
     1981 atacagtttg aagtaatatg tgcctgcctc ttccatatac acatacgaag ttcccgttcc
     2041 tcttgtttta tacaaggttt ccgccgcttc gctgccggca attaatcgtg caccttcata
     2101 ccagtcgcat ataagttttg ctcccgagaa agatgcatta cgtatgctga gcttgaccgt
     2161 ctgcccgggc ttgtccgttt ttaccattaa ccagtccaca tcatttgatg cggatatggt
     2221 aacagttttt gcaactcccg gagtaagcat taccgcctga tcataagaat catttccttc
     2281 caagctgtcg ggagggataa tgtcggttat aagggtgact ttattgagaa ccgcttctct
     2341 cgcggacggt gtcaccttta agtagtatgt ctgtgtctta tcattcttat gggtataata
     2401 tttgttgaat tcggtagatg tgaacaatac cggtgcggta tccccgttct taatcagatc
     2461 ttcttctctg taaagtgcga catttactcc tttaccggaa ctgatatcat tccttattat
     2521 atattgcaaa gcctgatctt tttgtaagga tatcttaaac cagtctatat catttatggc
     2581 cgagatatct ataatgtacg gtttctttat atctatcggt gttgctcttt cccagacatc
     2641 attgtcttca tatatatcat ttacgattgt agtgtaattc aatttaatat tgctgccgat
     2701 tgcactgttt cctattaccg aaactttcag ataataagat ccgcctttac ttatcttata
     2761 tgccgacggt ttgttcagca tgtaggttcc gaacagtctc tccgcattat tgccctgact
     2821 taaaacatct tctttgtagt acagagccga tataacgccg gttgcagcct gatcgtcgca
     2881 acttatcgtc agaatgacgg atttaccttc cgtcatgggt tccagttcaa accacttttc
     2941 actgaacgaa tcactgaagg ataaggaatg tgtcgtattg ccgtatattt ttacggaatt
     3001 ttcccacggt cccttcatca catcctctgt tgcaggttgt tgaacggtag cttgttcaga
     3061 ctgtactctt tcttcagtgt taactattac ggcatcatca gtaggaaatg atgctaatgt
     3121 tatgacgcta aaaacaagaa ctaataacag aattcctatg caaactatat tttttttcat
     3181 tatatccctc cgaatgatgt ataaaggata tactaatata ccgcaattgt aaacacaaaa
     3241 ggagccgttt gcggacatag ttcagaaaaa gttcacaatt aatcataaca atcaaattag
     3301 tcattaaagc cctgtcttcg gtatttaaaa acaatgatgt ttatgctaac atatcgatag
     3361 gaggcagcat gagaacggtt ttgataaccg gtgcttcaaa aggaatcggg cgacaggcaa
     3421 cggaggattt tttggcagcc gggtacaatg ttatcgcaaa tttccatacc acaaaaatgg
     3481 ataaatcgga atatccctcg tttcaggagc atatcgaggt gataaaagcc gatgtctccg
     3541 actatggtca ggttgaagaa atgttcaaaa aaggacaagt tagattcggc aacattgatg
     3601 ttgttataaa caatgccgcc gtaaactatg cgggacttct ttcccggatg gaagaaaaag
     3661 actgggacag gattatcaat gtaaatctta agggaatttt taatgtatgc aaatgtgcgg
     3721 taccgtccat ggtatacaga aaagcgggac atatcataaa tatatcctcc atgtggggca
     3781 taaccggtgc ttcctgcgag gttgcctatt cggtatccaa agccggtgtc ataggcttta
     3841 caaaagcgct ggccaaagaa ttggctcctt cgaatgtata tgtcaactgt gtcgcacccg
     3901 gcataataca gaccgacatg aataaagcct atactcaaca ggaattggaa gagatgattc
     3961 ctttgggaag aataggtaaa tgcagggatg tatcggaaat gcttcttttc ttagccgatg
     4021 aaaagacctc gtttataacc gggcaggtct tttcggtaaa cgggggaacg gttatttagt
     4081 ttaaggacag ttcataattt caatctacca tatgatacga ttgaagtcgg gagaggcaaa
     4141 taaatgatga gaagattaag agaattcttt atgaacagat acggaacgga ccagctgaac
     4201 agggctttaa tggttgtgta tgcccttttc ttttttgttt acctgataac caaaaataat
     4261 gtgtttttgt ttattgccct ggctgttgcc ctgtatcagg tattccgaac tctatcaagg
     4321 aatttcactc aaagagcaaa agagaatcag tttttcttaa aatactggaa tccccttaaa
     4381 ggtaaatatt tttccgttaa gaagaaagct accgacaaaa cacacagata ctataaatgc
     4441 cccaaatgca aaaccgaggt tcgcgtacct aaaaacaaag gtaagataag aatcacctgc
     4501 cccaaatgca gtgagcagtt tataaaaaag acttgaaaaa gcggtttaac cgattcaaca
     4561 ggtattgcct tggcatagaa ttatttgaga gtataagtta aaatagagtg gaaataataa
     4621 taaattaaat ggggggttgt tatggaattt ctaaaaacat tggaacagct gaggactccg
     4681 ttctttgatc agtttttcct gttagtcaca aaaatgggtg aggaactttt cttcaccgtg
     4741 gtaatattga ttgtcatatg gtgtatagat aaaaaaaccg ggtaccggat gtttttcata
     4801 ttcttactgg gttcctttgt caatcagatg cttaaattaa tctttaaagt cccgcgcccc
     4861 tggacatatg agaatgccgc aaagccggtg gaaggctcgg tggaagcggc aaccggctac
     4921 tcttttcctt cgggacacac ccaacagggc atctgtcttt tcggatcggt agccatgttt
     4981 cttaagaaaa cccgggtgta tatagttatg gtaattctta cacttcttgt ggccttttcc
     5041 agaatgtacc tgggagtcca tactcctgca gatgtattta catctttagt tatcggagta
     5101 gtgcttttat tgctctgcta ctacgctttc cgtgttgcag ataagcataa atacggggat
     5161 gaatatcttt acggtattgt tgcatttgct atcgtaattt cacttatagt gctctcgttt
     5221 gacaaaaccg gctcaccgga atatctgcac ggaatagaaa acggatggaa acttttaggc
     5281 tgctgtatcg gtattatagt ctccataatc gtagacatca aatacacaaa ctttgaagta
     5341 aaagccaagt ggtggcaaca gattataaag ttggttgtgg gtctgggaat ggtattggtt
     5401 ataaaatcct tcttaaaaca gcctcttttg gacctgttca accaaagtcc ggttgccgac
     5461 ggaataaggt atcttcttat aattctgttt gccggatgtt tatatcccat gctctttaag
     5521 atatttaaaa gaaacagtaa agcagacagc tgatgctgtc tgccttttac tttatgcttt
     5581 tttgttgagt tttacttatt ccgcttcata aggttttata tatatatttc ccgtggaagt
     5641 cttaaggttt atgtttgcgt ctgccgaatg agaaaccaat acggggattt ccgcatcgaa
     5701 tttacctaca tcggtgctgt aagaaacaac aagtttatcg gtttttacta tgtacaggta
     5761 tatggagccg gtgtcggtat ccgcatatat actgtctggc ggagctgcac attttattgt
     5821 aatgcttccg gtattgcttc tgaactctcc cgttttgaag ctgccttcgg tttccaccat
     5881 gcccgtgtcg gatttttgtg taatggactt tactttgtaa tttttgcata ttactctgcc
     5941 gacatccgat ttgcagtaca gcgtttccgt tattacatca tttgcctcaa ttcttcccgt
     6001 atcggtctcc gcattgattt tttcctccgc tgttatcctg tttaaagata tattaccggt
     6061 atcgctttta accgtaacac tcttggcctt taattccgat gcggttatcg aagaagtatc
     6121 ggtattcaga gtaactgttc tgcttgcgtt gagattgctg acattaattc ttccggtgtg
     6181 tgtttcaaga taaacaatgt cagccgttat atcctgtgcc gttatatcgg taatctgaca
     6241 atccgccttt aatgcggata atgactccga taatgttttg ggaatccata tgactacttt
     6301 tttgggagct attttgaata ttgactcgtt ttccagctca ctgattttca aaatgccgtc
     6361 ttttacggta taagtaagat tctctccttt ggctaaatct ctctgacagc tttccgcaat
     6421 cagtatcttt tcatgatcat gtaccttaaa ttccaccgct ttggttatcc actttacctg
     6481 tatctctttt attccgtcgg catttgtctc atatcttgcc ttttcttcaa actgtcctct
     6541 gcgaatctca attctgtgtc cttcgacaaa cgaaaaacta agcccgctga acccgtgttc
     6601 cgctatatac catgctccaa ggcctataat tactacaatt attaccagta tagctatggt
     6661 cctgattatt tttgatgatg ttgcattcat tttattcacc tcactactta gcttttccaa
     6721 gccagatatc caatatggtt tcgattgcag ctgcaagcag gaatataagc catgttaaat
     6781 gccatgcatt tgtctgaaag ctgacaatga aatacataat caaggttata agccataaaa
     6841 atcccgatac cgattttctt atgcttcttt tcctctctat ttccgccatt ctgtcggttt
     6901 gagtgttgct atgcctgtac cccggtttat ttgtcctgtt gtatattgca agtattatgc
     6961 ctatggccac caatattacc gcaatcatga catttatgag cacgctcttg tatgacagcg
     7021 ccataaccaa cggaaccgcc gcaagcacga aaatcattgc gaccaccgat ctcagagcga
     7081 gatatttctg cctcttggaa tcgtcgggag ttatctcctt ttccagttcg gcaaaaagtt
     7141 ccgataagtc tcctatacta tctatcgctg tcttatacgc ttcatccttg ctttttcctt
     7201 ccgttacaag atcattgtat ttgtccttga gattctgaag tatttcttct ttaacctcat
     7261 atgccttcgc cgtcgcaggc gcatttctaa acaagttatt cacgtacgcc tttaacttat
     7321 tctccatctt ccatcacccc cgtatcaatt agtttctcaa tcatgttctt tgacctgttc
     7381 cagtcactta ataaattaat atattcctct tttcctcttt cggtaataga gtagtatctg
     7441 cgtcttgcag tcacattatc ttcaccccag taggaagtaa taaatccggc ttcttccagt
     7501 cgcttgaaag ccgtatacag cgtcgcctcc ttgaattcgt attcgccggc ggtttttgca
     7561 gatatctctt tgtttatctc ataaccgtaa ctgtcctttt tcatcaagct tgccagtata
     7621 atcgtatcgg tatttcctct tataagatct gatgctatcg tcactctatc tcctcccttc
     7681 gttaagtatt tcaccttacg tgatacataa tagtataata tacttcgcct gtcaaggtat
     7741 tttttaaaaa ttttttattt tttctttctc tttatttttt ccctttttta tacggttgta
     7801 aatacagaaa attaacagtt atatccaata taaaaagttc gtttctttta cttttctgtg
     7861 gataacttat ttttgaattt atcgcatatt tatcgtataa attttgattt tacctttctt
     7921 atccacaaaa agcggggtta gcctgtggat aactttgcat taaaaaatgc cttgtttatg
     7981 ggcattttta tatgtatgaa agttatacgg tttgtttgtc cacaagtact gtgagtttta
     8041 ttcttccgct tcttcttgct ctttttccgt aattgttgcg gcaaccacgg cattatcttc
     8101 ctcaattctc ataagagtaa ctccttgagt tgctctggat aagatgctta tctgtcctac
     8161 atccagccta atcatatctc cctgcagaga cataagcaga agatcatcct ggtccgatgc
     8221 tatcatggaa gctataattg ttccggttct ttcagtgact ttgtagttta tgacgccttt
     8281 tcctgctcgt ttttgtatag gatactcgga cacgaaggtt ctcttgccga aaccgttctc
     8341 cgtgacggaa agtatggttt cttcttcctt taccagactc atgcctacca cataatcgtc
     8401 atcgtctaac cttattccta taacgccttt tgccgatctt ccggtttctt ttgtatcttc
     8461 ttcagtgaat ttaatagctt ttcctttatt ggttatgagc attacggtgt catcacctcc
     8521 ggaaagtctg acagctatca aatcatcttc ttctcttaaa gttaccgcta taataccgct
     8581 ctttcgtatg ttgacatagc ttataagggg ggttttcttt acaaatccca gtttggtggc
     8641 cataaacaga ttctgagtcg tttcaaagct ctctatcggc aggattgtag ttactttttc
     8701 attggaatcc aattccagaa gattgactat ggccgttcct cttgcggtcc tgctcgcttc
     8761 cggtatctga aagcctctga gtttatatac ttttcctctg tcggtaaagc agagaaccgt
     8821 gctgtgggtg cttgtggtaa atatatctac aatagtatct tcttctcttg tcgtaactcc
     8881 tattatgccc ttacctcctc tgttctggct tttgtaagta tctactggag tcctttttat
     8941 atacccgaat cttgtaagag tcagcactac cgtttcttct tttatgaggt cttcgtccag
     9001 caggtcttct tcggagtcct ctatggctgt tcttctttcg tcgccgtatt tcttctttat
     9061 aacttccagc tcctccatta caacccggtg agtgatgtcc tcatgctcca gtatgctgag
     9121 atagtacttt atcttttccg tcaactcatc atactcggtc tgaagcttca atatttccag
     9181 tccggtaagt cttcccagtc tcatgtccac tattgcctga gcctgtctgt cggtaaattc
     9241 gaatttttcc accaaacgga cttttgcttc cggctcgttg gggctcttgc ggatgg
//
LOCUS       DAOPWC010000008         9181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_7565_length_9181_cov_3.252685, whole genome shotgun sequence.
ACCESSION   DAOPWC010000008 DAOPWC010000000
VERSION     DAOPWC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7565_length_9181_cov_3.252685"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PKJ65_00395"
     CDS             <1..1109
                     /locus_tag="PKJ65_00395"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ40326.1"
                     /translation="EVETMIKSKSENIKIFRSKDSVFGYHGWPSVCRGLNGELLAVCS
                     GSRLGHVCPFGKVLLSRSYDEGHTWTAPEIIMDTPLDDRDAGITVFNKDKIIVTSFNH
                     FIERQSKFPQGGFKLAMRDAYFSNNHITKEMEKKYYGSTYVISDDKGLTWSEVMHAPV
                     TSPHGPTALKSGGLIYMGNYKEADENPAKYDKKLTFEKELLVFYSKDGIKWNYLSHVP
                     YVTEEGYEYYHCEPYILELKDKLVAFIRVQRGLFATFMTQSYDQGKTWSNPVRINKHG
                     SPPHALLHSSGAVILTYGYREKPFGQRAMISYDNCKTWSEEIILRDDGPDNDLGYPAS
                     VELKDNSILTIYYQKETSDSLCEIMGTIWELPKK"
     gene            1119..2153
                     /locus_tag="PKJ65_00400"
     CDS             1119..2153
                     /locus_tag="PKJ65_00400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ40327.1"
                     /translation="MALQKYIVSKDDRTYEAWPDIVLTKSGKLICVFSECEHHGNRDN
                     ARIVYCHSTDRGKTWSEKMYLTTKGTKEEFFNCARICVMPDDSLIIVADYIFGNENHK
                     AQTFIWKSFDDGITWQGPFDNPSSGIVPDKIRVLKSGRWIISSHFLDREINKLVQYLW
                     YSDDRGKTWSDAVIVAKDERYNLCETCILEVEDNVLVAFLRENSWEGYDCFKAFSYDG
                     GVTWDGVYRIPIPGCHRPVAGFLQDGTILMTYRFIQGGKQGWGKTTQNFMGCIFDKNT
                     ALAKERRQQQTRIFPIDYDRNPKSDLGYSGWVQFEDGTIYVVTYLLDDSPKGQIRGYS
                     FRMEDFLLPE"
     gene            complement(2236..3123)
                     /locus_tag="PKJ65_00405"
     CDS             complement(2236..3123)
                     /locus_tag="PKJ65_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNZ40328.1"
                     /translation="MENLSISEKSMKIIIKAQKDEETGALLYDYLAKKEKDPDNKKLL
                     HRMASDEISHAKTWKSYTKKDIKPNRLKLFFVKIISTVLGYTFTLKMMQKDEQFAQKD
                     YEILKVEVPDAIKIQEDEHRHEMELIAMLDEERLQYVGAMVLGLNDALVELTGAIAGL
                     TFALANTKVVALSGIITGISATLSMAASNYLAEKADGNPKALKSSVFTGVAYLITVAL
                     MVLPYLLLPADAYLEAFLTMIAVVILIILVFNYYISVAKDQPFFKNFLKMALISISVA
                     GISYLIGILAKNLLGIDVM"
     gene            complement(3127..4236)
                     /locus_tag="PKJ65_00410"
     CDS             complement(3127..4236)
                     /locus_tag="PKJ65_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40329.1"
                     /translation="MSKIIRGKETLSSKERIKRTFAFEPVDRVAMGYDTNTIAHENLC
                     KALKMDPKEKFAFLRYIGVDHAGAWFKYIGEKIYPDIPGRNRSSEHGAVTKYIQNQYG
                     GYWDFCDFPLLDVDDEVIYNYPFPNPDDFDYEFYKQRVDYLCDMGFAISIGDPGTADI
                     MNTTGMLMGVEQALINICTDHEATLHMIDKRLNMQLKRLERILELCKGKFDFMWLGED
                     LGSQHAPLISMDLYRRVLKPRHQKFIDLAKAYNLPVIIHTCGSSSWVYEEFLEMGMNG
                     VDTLQPEAKNMSPQYLTDHFGGRLVFRGCISTAGPLAYGTVEETKKVCQETLEIMSKH
                     GGYIFAPTHAIQDNTPPENTIAMYNTAHAFRKKGK"
     gene            complement(4233..5126)
                     /locus_tag="PKJ65_00415"
     CDS             complement(4233..5126)
                     /locus_tag="PKJ65_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ40330.1"
                     /translation="MKIATQSAAVDRIFGPEKAFSVLKGAGFEAVDFPLDNEVRLNLP
                     YPALKKYFTDIGKYAADSGLEIGQTHAPFYGPDKYMTQYDEVIEIQKRAIAATSFLGS
                     EYVIIHPIMTKERKYNNDYERNKEMILKYYTDLIPALKEHDVYLAVENMFSWDSVEKC
                     ICPTACTTAEEMAEYIDELGERFVACLDIGHINLIHQKGYEHVNFAHMIQTLGHRIKT
                     LHVHDNDGINDEHLPPFAGNIDWNLVMTCLKEIGYSGNLSLEADNFLTYFRPEQFEKG
                     QKVMYANAKRLYDMFVQGGTK"
     gene            complement(5458..6816)
                     /locus_tag="PKJ65_00420"
     CDS             complement(5458..6816)
                     /locus_tag="PKJ65_00420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ40331.1"
                     /translation="MLKNKRIRIILISTLVALVISALYMLGIISDEIKLFKMEATSVL
                     DNQVASIRNEFMVNINSVYELRALVYGARGKYVDPQIIGERILKNPNIRNILYAPDSV
                     VTNVYPLEGNEAVIGLDLLSPENKSCEDAKLAVQSEDVVLTGPYELKQGGQAFSARLA
                     VRIPDENSQLKYSGLVSVTMNFPDLISESTNNLHNSLKYDFILEKRLNDSDEYIEIHS
                     GRVSKYDDYISSDFSVSNMDFRLSIVPQSGWYDKSGIMIKILAFVVVSLAVGIIMGFI
                     FAARQKLIEKSRTDALTGIYNRAGGTHAINTIIKNNLYKSGAFIMMDLDNFKSVNDQL
                     GHQMGDEVLITTAAILKNSIRATDILARLGGDEFMLYLPFEGDSDFLDKKLEEIRIKM
                     HRDIKGTEGVVTVSSSIGVTFYDKDKNFNTLYEQADKALYQSKESGKNRITIYEAKSD
                     IG"
     gene            7345..>9181
                     /locus_tag="PKJ65_00425"
     CDS             7345..>9181
                     /locus_tag="PKJ65_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40332.1"
                     /translation="MKRIFVIILAICMLLTSCSPLPMIMSAESNSQEQFMAATAVLQN
                     TNTLSSIQAMEYTQNDLYNRPYEPAYDYPFLAPSVDWGQWVAGQAPATFSPDKISYTL
                     ADDVVKVDSGITKHIASAGQLMSGSMKQLQPPNQIIANGGYIRQNILDAAVAKTGGVI
                     KNGTVVVDESTGTAFKVVSPTIFTGLFQADEQLKEMIKPLEGTYGLTTPQLHEILKDF
                     TLEEDTIQLTRANISGFAPNIERNICLPKPEDNYADIRTLGSGDEFNVDMEKGFKYIS
                     NQSLLALKFEKVRLDATVSNTKIAVTVTGGLGIDNIELTGRYSSFGGYEIGLSLKQEC
                     YLIIELDAEVHEEIRIPLLAIDVPFLIGRVSGGLYAIVGMDGDIRLDIAMREWTPAQV
                     GVSGGTSFCVPTSVTPHIYQDIKCDGNVQLAAKINGYMKFGPMVSLEVFGLDLVGTGV
                     LLGTGVSVVKEGAYLDVTLYAILNVYVSFLGKIYNLANFRPAILNRRQTDTAGLIIKF
                     QEVFLHPKRAGGTIQKEPDKSGAPYEPAGNVEYRIWVVPKNINVDLNNPSTFNQIGIE
                     KYPKTGYARTNAWGEFIQYPDGEALRSKSKVYLELKWEGKTYYSDP"
BASE COUNT         2659 a         1982 c         1850 g         2690 t
ORIGIN      
        1 atgaggtaga gactatgatt aaatcaaaat ccgaaaatat aaagatattc cgctcaaagg
       61 attcggtatt cggatatcac ggatggcctt cggtatgcag aggtttaaac ggtgaacttt
      121 tagcggtttg ttcgggtagc agattagggc atgtatgccc gttcggcaag gtactgcttt
      181 cacggagcta tgatgaagga cacacctgga cagcccccga gattataatg gacacacctt
      241 tggatgacag ggatgcgggt atcaccgttt tcaacaaaga caagataatt gtcacatctt
      301 tcaatcactt tatcgaaaga caaagtaaat tccctcaagg gggctttaaa cttgccatga
      361 gagatgcata tttctccaat aatcacatca caaaggagat ggaaaagaaa tattacggct
      421 ccacatatgt tatctcggac gataaaggac tgacttggag cgaggttatg catgcaccgg
      481 taacctcccc gcacggaccg accgcattga aaagcggcgg acttatatac atgggcaatt
      541 acaaagaagc cgatgagaat cccgccaaat atgataaaaa actgacattc gaaaaagagc
      601 ttttagtctt ttacagcaaa gacggtatca aatggaacta tctttcgcat gtcccctatg
      661 tgacagaaga aggatatgag tattatcatt gcgaaccgta tattctggaa cttaaggaca
      721 aacttgttgc tttcatcagg gtgcaaagag gtctcttcgc aacctttatg acccaatcat
      781 acgaccaagg caaaacatgg tccaatcccg taagaatcaa caaacacggc tctcctccgc
      841 atgctctttt acacagcagc ggcgcggtca tattgacata cggttacaga gaaaagcctt
      901 tcggacaaag ggctatgatt tcttatgata actgtaagac ctggtcagaa gaaataattc
      961 ttcgagatga cggaccggac aacgatctgg gatatccggc ttcggtggaa ttgaaggata
     1021 acagtatttt aacgatatat tatcagaaag aaacttccga ttctctgtgc gaaataatgg
     1081 gaacaatatg ggagttaccg aaaaaatagg agggtactat ggcgctgcaa aaatatatag
     1141 taagtaagga cgatcggact tatgaagcat ggccggacat tgttttaact aaaagcggaa
     1201 agctgatatg cgtattctcc gagtgcgagc accacggcaa cagagataat gcaagaattg
     1261 tatactgcca cagtaccgac cgcggaaaga cctggtcaga aaagatgtat cttacaacaa
     1321 aaggtacaaa agaagagttc tttaactgtg cacgcatttg tgtaatgccc gatgattcat
     1381 tgattattgt tgccgactat attttcggta acgaaaatca taaggcacag acatttatat
     1441 ggaaatcgtt tgatgacggc attacatggc agggaccttt tgacaatccc tcatcgggaa
     1501 ttgttccgga taagataagg gtgttaaaaa gcggtagatg gataatatcc tcgcattttc
     1561 tggacagaga aattaataaa ctggtgcagt atctgtggta cagcgatgac cggggaaaaa
     1621 cctggtcgga tgcggtaatt gttgccaaag atgaacgata taacttatgc gagacctgta
     1681 ttctggaagt ggaagataat gtattggttg cgttcttacg ggagaactcg tgggaaggat
     1741 acgactgctt taaagcattt tcctatgacg gcggggttac atgggacggt gtatacagaa
     1801 tccccattcc cggctgtcac agaccggttg cgggcttttt gcaggacggc accatactta
     1861 tgacgtacag gttcatacag ggcggaaaac agggctgggg caaaactacc cagaacttca
     1921 tgggatgtat atttgataag aataccgctc tggcaaagga gcggcgacag cagcagacca
     1981 ggatatttcc tattgattac gacagaaacc ccaaatccga tttgggctat tccggatggg
     2041 ttcagtttga agacggtaca atatatgtgg tcacttatct tctggacgat tctcccaagg
     2101 gacagataag aggatattcc ttcaggatgg aagattttct attaccggaa tagtttccaa
     2161 aacaattaat cagttaccga atggatgaat aactgcataa aagattaaat aagcaaatat
     2221 actaagtctt ttatactaca ttacatctat gcccaaaagg ttcttggcaa gtattcctat
     2281 taaataggat atgcctgcta ccgatatgct tataagggcc attttaagga agttcttaaa
     2341 aaacggctgg tctttggcta cggaaatgta gtagttgaaa acaaggatta tcagtattac
     2401 cactgctatc attgtcagga aagcttccag atatgcatcc gcaggcaaca gcagataagg
     2461 caaaaccatc agtgccaccg ttataagata agccactccg gtaaatacac tggatttgag
     2521 tgctttaggg ttgccgtcag ccttttccgc aagatagttg gacgcagcca tggataatgt
     2581 agcggatatt cccgttatta ttcccgacaa tgcgactacc ttggtattgg caagagcaaa
     2641 ggtcaggcct gcaatggctc cggtcagctc caccagtgca tcgttaagac ctaagaccat
     2701 tgctccgaca tactgaagcc gttcctcatc taacatggct atcagttcca tttcatgcct
     2761 gtgctcgtct tcctgtatct ttatggcatc gggaacttca actttcagta tttcatagtc
     2821 cttctgagca aattgctcat ccttctgcat cattttaaga gtaaatgtat atcccaatac
     2881 ggtacttata attttgacaa agaaaagttt caacctgttg ggttttatat ctttcttggt
     2941 ataactcttc catgtctttg catggcttat ctcatccgaa gccattctgt gaagtaattt
     3001 tttgttgtcg ggatccttct cttttttcgc aagataatca tataataaag cacccgtttc
     3061 ttcgtctttc tgggctttta tgattatttt cattgatttt tcactgattg acagattttc
     3121 catgtgctat ttccctttct ttctgaatgc atgtgccgtg ttatacatag ctatggtgtt
     3181 ttccggcggc gtattatcct gtatcgcatg tgtcggagcg aatatatatc ctccgtgttt
     3241 actcattatt tccagagttt cttggcatac ttttttagtt tcttccaccg ttccgtatgc
     3301 aagcggaccg gctgtggaaa tacagcccct gaatacaagt cttcctccga aatgatcggt
     3361 tagatactga ggactcatat tctttgcttc cggctgtaaa gtatccactc cgttcatgcc
     3421 catttctaaa aactcctcat aaacccatga tgaacttccg caggtatgta tgataacggg
     3481 cagattgtat gccttagcca gatctataaa tttctgatgc ctgggtttta atacccgtct
     3541 gtataagtcc atggatataa ggggggcatg ctgagaaccc aaatcctctc cgagccacat
     3601 aaagtcaaat ttacctttac acagttccag aatcctttcc agcctcttca gctgcatatt
     3661 caatcttttg tctatcatat gcaatgtggc ttcatgatcg gtacaaatat taattaaagc
     3721 ctgttccaca cccattaaca taccggttgt gttcattata tcggcggttc ccggatcacc
     3781 tatggaaatg gcaaagccca tatcgcaaag atagtccact ctttgcttat aaaactcata
     3841 gtcaaagtca tcgggattag gaaaagggta attgtatatt acttcatcgt ctacatccag
     3901 aagcggaaaa tcgcagaaat cccaatatcc tccatattga ttttgtatat acttggtaac
     3961 cgctccgtgt tcggaactgc ggtttctgcc gggaatatcg gggtatattt tctcacctat
     4021 gtatttaaac catgccccgg catggtcgac acctatatac cgcaaaaatg cgaatttctc
     4081 tttagggtcc attttaagtg ctttacataa gttctcatgt gcaatggtat tggtatcata
     4141 acccatagct actctgtcta ccggctcaaa agcaaatgtt cttttgattc tttctttgga
     4201 agacagcgtt tcttttcctc tgattatctt actcattttg tccctccctg aacaaacata
     4261 tcatacagtc tttttgcatt tgcatacata accttctgac ccttttcaaa ctgctccggt
     4321 cggaaatatg tcaaaaaatt atccgcttca agactcaaat tcccgctata acctatctct
     4381 tttaagcaag tcattacaag attccagtca atattccccg caaaaggagg aaggtgctca
     4441 tcatttattc cgtcgttatc gtgtacatgc aatgtcttta tcctatgtcc caaagtctgt
     4501 atcatatgag cgaagttaac atgctcataa cctttctgat gtataaggtt gatgtgccct
     4561 atatctagac aggccacaaa cctctccccc aactcatcaa tatattccgc catctcttcc
     4621 gccgtagtac aagcagtcgg acagatacat ttttccactg aatcccagga gaacatattc
     4681 tccactgcaa gatatacatc atgctctttc agtgcgggaa tcaaatcggt ataatactta
     4741 agaatcatct ctttattacg ctcatagtcg ttattgtact ttcgctcctt ggtcattatg
     4801 gggtgtataa ttacatattc agaccccaga aaacttgttg cggctatagc ccttttttga
     4861 atctcgataa cttcgtcata ttgcgtcata tacttgtcgg ggccataaaa aggtgcatga
     4921 gtctgaccta tctccaaacc cgaatcggca gcatattttc ctatgtctgt aaaatacttc
     4981 tttaaagcgg gatacggaag attaagccga acctcattat ccagcggaaa gtccactgcc
     5041 tcaaaacctg ccccctttaa aacggaaaaa gccttttccg gtccgaatat tctgtctact
     5101 gccgcacttt gtgttgctat tttcataaac tacctcataa actccaacca agcattaaat
     5161 aataaaatac aactctatct tatgatagta gcatagcatt ttcaagacta tttcacttaa
     5221 cattcaattt gagaagcatt cataaacagt taaatctgct cctgcttttg attgagtgta
     5281 cttaactctt tttctcttcc gataaacata cgaacccttc attactgtat cacgataaac
     5341 tttatcgtaa tacagttaat aatacaagtc ggaaagtatc aatatatttt tccgattctc
     5401 ttaacttaaa agcatgtaat ccatacttat aggaagagac tgatcccgga taaatcgcta
     5461 tccgatgtct gattttgctt catatatcgt tatccggttt ttcccgcttt cctttgactg
     5521 atagagggct ttatcggctt gctcataaag ggtattaaag tttttgtctt tatcgtagaa
     5581 ggttacgcct atactgctgc ttacggttac gactccctcc gttcccttaa tgtcgcggtg
     5641 catcttaata cgaatttcct caagcttttt atctaagaaa tcggagtcgc cctcaaacgg
     5701 aaggtacagc ataaactcat ctcctcccag tctggccaat atatccgtgg cacggatgga
     5761 atttttcagt attgccgctg tggttataag tacctcatcc cccatctgat gcccgagttg
     5821 atcattgaca ctcttgaagt tgtccaagtc catcataata aaggctccgc ttttatagag
     5881 gttgttcttg attatagtat ttatcgcgtg agttcctccc gccctgttat atattcccgt
     5941 cagtgcatcg gttctggatt tttctatcag tttctgcctg gcggcgaaga taaagcccat
     6001 gattatacct acagcaagag acactacaac aaaggccagt atttttatca taattcccga
     6061 tttatcatac caaccggatt gcggtactat ggataatctg aagtccatgt tggatacgga
     6121 aaagtcggaa gatatataat cgtcatattt cgaaactctg cccgaatgga tttcgatata
     6181 ttcatcactg tcgttcagtc ttttttccag tataaaatca tattttaaac tgttatgcag
     6241 attatttgtg ctttcggata ttaaatcggg aaaattcatg gtaaccgata ccaatccgct
     6301 atacttcaat tgggaatttt catcgggaat cctgaccgcc agtcttgcgg aaaaggcttg
     6361 tccgccttgc tttaattcat acggtcccgt tagaactaca tcctccgatt gcaccgccaa
     6421 tttagcatcc tcacagcttt tgttttcagg actcaataaa tccaatccga taacggcttc
     6481 attaccttcc aaaggataga catttgttac tacagagtcg ggcgcataca atatgttacg
     6541 tatattggga tttttcaata tccgttcacc tattatttgc ggatccacat acttgccccg
     6601 ggctccgtaa acaagggctc tgagctcata aacggaattt atattgacca taaactcatt
     6661 tcttatggaa gctacctgat tatccagaac agaagttgcc tccattttaa aaagctttat
     6721 ttcatcggat attatgccca gcatgtacaa ggcacttatc accaatgcaa caagagtgga
     6781 tatgagtatt attctgattc ttttgttttt caacatatta gcgatacctc tgaaactttt
     6841 tatacaaaac cttatattac ttttcgcgaa gatggtgcgc aaatctcaat aatattgtac
     6901 aacagtgctt tgattttttc catcgggatg taaaccgtta gaacggattt aacacggttt
     6961 ttcaatagaa caactcaaat attgaccaaa gacacccttt ttatgtcaac tttttacaaa
     7021 tatcatctgt taaaaataaa aaaatcattg tataatgaaa tcgcaattag aattgccttg
     7081 aatacgtaaa aagaagcaca cctcggatgc tgatgtcatt tcaattacga caccgtgtta
     7141 aaagcataac ctttttgaca taaaagcaaa tgcgatgttc cgaagagcct gtcagcatgt
     7201 ggggagcaaa ttactttgtc aatcgaatat accggaaact cggagcagca gtaaaaccga
     7261 gcttatctta cagtatttct tatttccggt accggatcat agacaaacga ataacataat
     7321 attcataatc gggaggacac ccacatgaaa agaatttttg taattatact ggcaatatgt
     7381 atgctgctta cttcctgctc tccgttgcca atgataatgt ctgctgaaag caactcgcag
     7441 gaacagttta tggctgcaac tgcggttttg caaaatacaa atacattatc gtcaattcaa
     7501 gccatggagt atactcaaaa cgatctttat aataggcctt atgagcctgc ttatgattat
     7561 cctttcctgg caccgagcgt tgattgggga caatgggtag caggtcaggc accggcgact
     7621 ttctcgcctg ataaaatttc ctacacgctt gctgatgatg tggtaaaagt agattccggc
     7681 atcacaaaac acattgcttc ggccgggcaa ctcatgtcgg gcagtatgaa gcaactgcaa
     7741 ccgcccaatc agattattgc caacggaggc tatattcgtc agaatatctt ggatgctgca
     7801 gtcgcaaaga caggcggtgt tatcaaaaac ggaacggtgg tcgtggacga atcgaccggt
     7861 accgctttta aggtggtgtc ccccactatt tttacaggac tttttcaagc cgacgagcaa
     7921 ctcaaagaaa tgataaaacc gctggagggg acatatgggc ttaccacgcc ccaacttcac
     7981 gaaatactta aggactttac actggaagaa gataccattc agctgacaag ggcgaatata
     8041 agcggatttg cgcccaatat cgaacgaaat atttgtctgc ccaagcccga agacaactat
     8101 gcagatataa ggacactggg ttccggtgac gagttcaatg ttgatatgga aaaggggttt
     8161 aaatatatct caaaccagtc tctgcttgcc ctgaaatttg aaaaagtaag gttggacgcc
     8221 actgtcagca acactaaaat cgccgttacg gtcaccggcg gtttgggcat tgataatata
     8281 gaactgacag gcagatattc ctctttcggc ggctatgaaa taggtctcag cttaaagcag
     8341 gaatgctacc ttattatcga actggacgcg gaagttcatg aagagatacg tatacctctc
     8401 ctagccattg atgtaccctt tcttatcggt cgggtaagcg gcggtctcta tgctattgta
     8461 ggtatggacg gcgatattcg tctggatatt gctatgcgtg agtggacacc ggcacaggta
     8521 ggagtaagcg gcggtacatc attttgtgta cccacaagcg ttacaccgca tatctatcag
     8581 gatataaaat gtgacggaaa tgtacagctt gcagctaaga taaacggata tatgaagttc
     8641 ggtcccatgg tgagcttaga ggttttcggt ctggatttag tgggaacagg agtcttactg
     8701 ggaacgggtg taagtgtggt aaaagaaggt gcctatcttg atgtcactct ttacgctata
     8761 cttaatgtct atgtgtcatt cctcggtaaa atttacaatt tagccaattt tcgacctgca
     8821 atactgaatc gacgacaaac cgacacggca ggccttataa tcaagtttca ggaagttttc
     8881 cttcatccca aacgagcggg cggtaccatt caaaaagagc cggataaatc cggagctcca
     8941 tatgagccgg ccggcaatgt tgaatatcgg atctgggtgg ttcccaaaaa tattaatgtc
     9001 gacctgaaca acccgtccac ttttaatcag ataggtattg aaaaatatcc gaaaaccggt
     9061 tatgcccgta ccaacgcgtg gggcgagttt attcagtatc ctgacggaga ggcccttcgc
     9121 agcaaatcca aggtatatct tgaacttaag tgggagggta aaacctacta ttccgatcct
     9181 g
//
LOCUS       DAOPWC010000009         8907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_7862_length_8907_cov_3.986331, whole genome shotgun sequence.
ACCESSION   DAOPWC010000009 DAOPWC010000000
VERSION     DAOPWC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7862_length_8907_cov_3.986331"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKJ65_00430"
     CDS             complement(<1..1194)
                     /locus_tag="PKJ65_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ40333.1"
                     /translation="MENEKLCDVSKIIPVDIEEQMRKAFIEYAMSVIADRALPDVRDG
                     FKPVHRRILYAMHLLGFYPDKSHRKCAATVGEVLGKFHPHGDAAVYDAMVRMAQDFSL
                     RYPLIDGHGNFGSRDGDPPAAMRYTESRLTRIAVEMLADINKETVDFKSNFDDHEMEP
                     VVMPSRFPNLLVNGSSGIAVGMATNIPTHNLTEVIDAIFAVIKNPDITIEELIEIVKG
                     PDFPTYGTIMGRSGIYQAYKTGKGRIVVRGKAEIEEHANGRQRIIIHDLPYQVNKAKL
                     IETIADMVKNKSIEGISEINDESDRNEEVRIIITLKKDANANVILNRLYKQTKLQDAF
                     NVNMLALTPSKTGYEPKTLNLKQMLEYYIQHQVDVIRRRTKFDLDKAQTRAHVLEGLI
                     KAVDIL"
     gene            complement(1390..1812)
                     /locus_tag="PKJ65_00435"
     CDS             complement(1390..1812)
                     /locus_tag="PKJ65_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40334.1"
                     /translation="MTEDRRNISAEEKAKAIRKAKIEKIVTISILVLLVLGIVSALIF
                     KSIVGTWWVEIQTDSTTRGAYIYFGGDKTYETFMYGVDEWAEKGTYELKGTSVNGYII
                     FNPENGRQYIRQYNIRGTRLKIFNPDDSFVSFLRNRTD"
     gene            complement(1862..2665)
                     /locus_tag="PKJ65_00440"
     CDS             complement(1862..2665)
                     /locus_tag="PKJ65_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNZ40335.1"
                     /translation="MKEILNEILIPVSIVGGIALFFGVILTIAGKFFEVKKNEKAEAI
                     REILPGANCGACGYSGCDLYAEAVAEGKAEGNLCIPGTNASAIKIGEILGVKVATQKT
                     RVMKAICCPDVSKKYTYSGIKTCAAASLAYRGENSCLYSCLGYGDCVAACPNGAIMMK
                     DGAPVIDPVICTECGLCAKTCPRNLIRYLIGEDGYYVACASEDSGRITGKNCKNGCIA
                     CNLCVKACQDDAIHVINNVAVIDYEKCTRCGKCYDVCPKKIIKKFCSSY"
     gene            complement(2676..3269)
                     /gene="rsxA"
                     /locus_tag="PKJ65_00445"
     CDS             complement(2676..3269)
                     /gene="rsxA"
                     /locus_tag="PKJ65_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNZ40336.1"
                     /translation="MNTDTFRMIIYISFGAILMNNFVLSNFLGICPFLGVSKKLSSAV
                     GMGVAVIFVMALASVVTYLTNTYLLVPYNLEYLQTLTFILVIAGLVQFIEMFMKKTMP
                     SLYKALGIYLPLITTNCAVLGVALLNVQYGYNLWQSALYGATGGLGFMIATIIFAGIR
                     ERLDMNDVPKYFKGLPIALITAAFLSMAFTGFKGMFG"
     gene            complement(3266..3928)
                     /locus_tag="PKJ65_00450"
     CDS             complement(3266..3928)
                     /locus_tag="PKJ65_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNZ40337.1"
                     /translation="MKNRSLQELSKGIIKENPTFILVLGMCPTLAVSTTAKNSFMMGI
                     AATAVLVLSNIIISLLRKLIPDTVRIPVYITVIAGFVTIVQLLMKAFLPELDKALGIY
                     IPLIVVNCIILARAEAFAGKNNAWYSLLDGLGMGLGFTLALTVIGTIREFLGNGAIFG
                     QEIIKNIPMPAIFVTPAGAFLVLGSLMALVSALTRKKTEIKDCADCPMQCEAKDKKGE
                     SR"
     gene            complement(3925..4458)
                     /locus_tag="PKJ65_00455"
     CDS             complement(3925..4458)
                     /locus_tag="PKJ65_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ40338.1"
                     /translation="MKKYIAPVLSLFLITLCVVGLLALTNRFTKGPIEAKEKEEAEKA
                     MNMVLSGGAPFTDISEEVMKLDINTKAKLKGVSKSAKGYVFTMVAKGYGGEMVVFVGI
                     NNEGAVENIVIGDNDETPNIGKNAELPEFTSQFTGFTRQDDIADNVDYITRATVTTDA
                     VISAVESALLIHAEVVK"
     gene            complement(4455..5375)
                     /locus_tag="PKJ65_00460"
     CDS             complement(4455..5375)
                     /locus_tag="PKJ65_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ40339.1"
                     /translation="MDKLIVSSSPHIKDKATTSRIMLDVIIALMPTAVLSVYYFSYIS
                     ALIIVASVSSCVLFEYLFNLITKKRHTETDLSCVVTGLILAMNMPPLKSSIILSVIGA
                     FIAIVVIKMLFGGIGQNIFNPAAAARIFLTTAFSSLMTVYVAPLTDGVTAATPLAIPK
                     GEMLPSYLDLFLGKTPGSLGETAAVTLIAGGIYLMIRKVISWEIPAIYIGTVALLSFT
                     FGQDPLYSILAGGLILGSIYMATDYTTSPVTRKGKIVYAAGLGLMTASIRKFGSNTEG
                     VAFSLLTMNALVPLIDKFTVKKTFGRIKKK"
     gene            complement(5368..5700)
                     /locus_tag="PKJ65_00465"
     CDS             complement(5368..5700)
                     /locus_tag="PKJ65_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ40340.1"
                     /translation="MMGTTAESYESPVIKQSNAFLFMTPEDLDFMEESACIRCGRCVE
                     ACPMGLMPLTMNRYALAKMTEELEKYNIMNCIECGSCSYVCPAKRHLVQSFRVGKDLV
                     RKAKVKNG"
     gene            complement(5721..6680)
                     /locus_tag="PKJ65_00470"
     CDS             complement(5721..6680)
                     /locus_tag="PKJ65_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HNZ40341.1"
                     /translation="MIIKVKNIKGVALPHRKGTSEIPTEILPIPKLVEIPMQQHIGAP
                     CKVLVGKGDYVYVGQVLGDSEKFVSAPIHSSVSGNVVQIKDIILHNGVKCESIVIETD
                     GKQEVLPGIDQPDISTKEKFLKAVRNSGLTGLGGAGFPAHVKLDTKERIPEALIINAV
                     ECEPYITCDYRAIIEEGGEIVNGVKLLLDKLAIPKAYIAVEDNKPAAFSLLKELTKDH
                     SGIQTVMLKTKYPQGAEKMLIYALTGKEVPMNGLPIDIGTVVMNVTSVLALYKYCKTG
                     MPLVSKRITVEGSGVKNRGNYQVVIGTHIKDIFEYCKVIKKKR"
     gene            complement(6782..7582)
                     /locus_tag="PKJ65_00475"
     CDS             complement(6782..7582)
                     /locus_tag="PKJ65_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40342.1"
                     /translation="MRRKTVIVIAVIIAIGFVMGLTSCTPYHKRPRPSINTANALEDR
                     YVIKEFPYDIATYGGEPIMKPTVSQFNNISRQKTLNARLSAFNEMIGRDPVQERNEQL
                     YQTYEIRQMNHYLFSCEYFTGFETTVTNKGVNMIIGSEIDGTLLMNFPALLGTNQESD
                     AWEEFMIFFNNARNAVGAKEITKAYMATDNVSFVFDGEDVTDLTLRVLYKVPDDTEQQ
                     EVPMSLNEIKGCLSERMQVYMSFTEDPSMTYEEYANTIAEYVLFLATE"
     gene            complement(7832..8626)
                     /locus_tag="PKJ65_00480"
     CDS             complement(7832..8626)
                     /locus_tag="PKJ65_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40343.1"
                     /translation="MKRKTVMLIAVILAIVFITGLASCTPHEIRISPSINTANALEDR
                     YIVVETEYNVATYGGEPIMKPTVAQFNNISRQKTLNARLNAFNEMVGRDSGQDRNEQL
                     YQSYEIRQINRYLFSCEYFTGFETTVTNKGVNMLVGSEINGTLLMNFPALLGTKQESD
                     SWTEFMVLFEEARKAAGAKEITKEYMATDNVSYVFDGDDLTDLTLRILYKAPDDTKQQ
                     EVPMKLNEIKKCFLEKIQIFMSFTEDPLMDYEEYANTILEYVLQQE"
BASE COUNT         2358 a         2044 c         1610 g         2895 t
ORIGIN      
        1 cagtatatca acggctttta tcagtccttc cagtacatgt gctctggtct gtgccttatc
       61 cagatcaaac ttggttcttc tccttattac atccacctga tgctgtatat aatattccag
      121 catctgcttg agattcaatg tcttgggctc atatccggtc ttggaaggag tgagtgcaag
      181 catgttgaca ttgaaagcgt cctgaagctt tgtctgctta tacagcctgt taagtataac
      241 gttggcattg gcatcttttt ttaaagtaat tattattctt acttcttcgt ttctgtccga
      301 ctcgtcgttt atttccgata ttccttcaat ggatttattc ttgaccatgt cggcaatggt
      361 ttcgataagt tttgccttat ttacctggta aggaaggtca tgaattatta ttctttgtct
      421 tccgtttgca tgctcttcta tttcagcttt gcctctgacg acaattcttc cttttcccgt
      481 cttatatgcc tgatatattc ccgaccggcc cattatggtc ccgtatgtgg ggaaatccgg
      541 gcctttaact atttctataa gttcctcgat ggttatatcg ggatttttaa taaccgcaaa
      601 aatagcatct attacttcgg tcaggttgtg ggtaggtatg ttggttgcca ttcctaccgc
      661 tataccgctg gaaccgttta caagaagatt gggaaatctt gaaggcatta caaccggttc
      721 catttcgtgg tcatcaaaat tggatttgaa gtccacggtc tccttgttta tatccgcaag
      781 catttccaca gctatcctgg taagtctgga ttcggtgtat ctcattgcgg caggcggatc
      841 cccgtcacgt gatccgaagt taccgtgccc gtcaatcaag ggatacctca gggagaaatc
      901 ctgagccatc cgcaccattg catcatatac cgcagcatcg ccgtggggat ggaattttcc
      961 taaaacctct cctacggtag cggcacattt tctgtgagat ttatctggat agaatcccaa
     1021 taaatgcatt gcataaagta ttcttctgtg aacgggcttg aatccgtcac gtacatcggg
     1081 caatgcccgg tcggctataa cgctcatagc atattctatg aacgcctttc tcatttgttc
     1141 ctctatatct accggtataa ttttggaaac atcacataat ttttcgtttt ccatagtgtt
     1201 tttttagtat ctctttcaca agttttatct taataattat accataaaac acctttccgg
     1261 tctatgcaaa acgggaatat gctcggctat cggaacggta aaaacaagtc ggaagttaaa
     1321 aagaaaaagc ctattcataa agctttttcg atgatgtctt ttatatgtgt tttaattgtt
     1381 ctctgccggt taatcggtcc tgtttcttaa gaaactgaca aacgaatcat ccggattgaa
     1441 tatcttcagc cttgttcctc tgatattata ctgtcttatg tactgtcgtc cgttttcggg
     1501 attaaagatt atataaccgt tgacactggt acccttcagt tcataagtcc ctttctccgc
     1561 ccattcgtca actccgtaca tgaaagtctc gtatgtttta tcgccgccga aatatatata
     1621 tgctcctctg gtcgtggaat cggtctgtat ttccacccac catgtgccca ctatagactt
     1681 aaatattaat gccgatacta tacctaaaac aagcaatacc aatatggata ttgtaactat
     1741 cttttcaatc ttcgctttgc gtatggcttt ggctttttcc tccgcactta tattccttct
     1801 gtcttccgtc atttatcaat ctcctttatt tacaatcggt cttcacattc tatcataact
     1861 gtcagtagct tgaacagaac ttctttataa tcttcttagg gcagacatca tagcatttcc
     1921 cgcaccttgt gcatttctca taatctatta ccgccacatt attgataaca tgtatagcat
     1981 catcttggca agccttcaca cacagattac atgctatgca tccgttcttg cagtttttgc
     2041 cggttatcct gccgctgtct tcacttgcac atgcaacata atatccgtcc tctcctataa
     2101 gatatcttat aaggtttctg gggcaggttt ttgcgcaaag tccgcattcg gtgcatatta
     2161 ccgggtcaat aaccggtgcg ccgtctttca tcattatggc accgttggga catgcggcaa
     2221 cgcaatcacc gtatccgaga caggagtaaa gacaggaatt ttcccctctg tatgccaaag
     2281 aagcggccgc acatgtctta ataccggaat aagtatattt ttttgataca tcgggacaac
     2341 atatggcttt cataactctt gttttttgtg ttgccacttt gactcccagt atctccccta
     2401 tttttattgc cgaggcattg gttcccggta tgcataaatt accttcggct tttccctccg
     2461 caacggcttc ggcatataaa tcacatcccg aataaccgca agccccgcaa ttggctccgg
     2521 gaagtatttc tcttatcgct tccgctttct cgttcttctt cacttcaaag aacttacctg
     2581 ctatagtgag tatcactccg aagaacagag ctattccccc tactatggaa accggaatca
     2641 gtatttcatt aagtatctct ttcaccgctt acacctcacc cgaacattcc tttaaatccc
     2701 gtaaaggcca tagacagaaa tgccgctgtt atgagcgcta tgggaagacc tttaaaatat
     2761 ttaggcacat cattcatatc caatcgctct cttatgccgg caaatattat tgtggctatc
     2821 ataaatccta agcccccggt ggctccgtac agagcgctct gccacaggtt atatccgtac
     2881 tgcacattca acaaagccac gcccagaacg gcacagttgg tggtaatgag cggaagatat
     2941 attcctaagg ccttatataa cgaaggcatg gtttttttca tgaacatctc tatgaactgc
     3001 accaatcccg ctattaccag tatgaatgtc agtgtctgca gatactccag attgtaagga
     3061 accagaagat aggtgttggt taaataagtt acaaccgaag ccagagccat tacaaatata
     3121 acggcaaccc ccattcccac agcggaactc agttttttcg atactcccag aaagggacag
     3181 attcccaaaa agttggagag aacaaaattg ttcatcagga tagctccgaa agatatatat
     3241 ataatcattc taaatgtatc tgtgttcatc ggctttctcc tttcttatct tttgcttcgc
     3301 attgcatggg gcaatccgca caatccttga tttcggtttt cttacgggta agagcgctaa
     3361 ccagtgccat aagcgaaccc agcacaagaa atgcgcctgc aggtgttaca aaaattgcag
     3421 gcataggtat gttctttata atctcctgcc cgaaaatcgc accgttcccc aaaaactctc
     3481 ttattgtacc tataaccgtc agtgcaaggg taaatcccag acccattccg agaccgtcca
     3541 acaatgaata ccaggcattg ttcttgcctg caaaagcctc ggctctagcc aaaataatac
     3601 agtttacaac tataagcgga atgtatatac ccaatgcctt atccaattcc ggcaggaatg
     3661 ctttcataag caactgtact attgttacaa agcctgctat tacggttata tataccggta
     3721 ttctgaccgt gtcgggtatg agctttctca gaagcgatat aattatattg gacaaaacaa
     3781 gtaccgctgt cgctgctata cccatcataa aagagttctt agcagtagta gagacggcta
     3841 acgtggggca cattccgagc accaatataa atgtgggatt ctctttgatt ataccttttg
     3901 aaagttcctg taaagatctg tttttcattt aaccacctct gcatgtatca aaagagcact
     3961 ttccaccgcc gaaataacag catcggtggt aaccgtcgcc cttgttatat agtcaacatt
     4021 atcggctata tcgtcctgcc ttgtaaaacc ggtaaactgg gaggtgaact ccggaagttc
     4081 cgcgttcttt cctatgttag gagtttcatc gttgtcccct attacaatgt tctccacagc
     4141 tccttcgtta tttattccta caaatactac catttctcct ccgtaacctt ttgcaaccat
     4201 agtaaagaca tatcctttag cgcttttgct cactcccttt aatttcgctt tggtgttgat
     4261 gtccaatttc atcacctctt cggaaatatc cgtaaaaggc gcaccgccgg aaagcaccat
     4321 gttcattgct ttttccgctt cttctttctc tttggcttca atcggtcctt tggtaaaacg
     4381 attggtaagc gcaagcagtc cgacgacaca gagggttata aggaaaagac ttaatacagg
     4441 agctatatac tttttcattt cttcttaatc ctcccgaaag ttttcttcac cgtgaatttg
     4501 tctatcaaag gaacaagcgc attcatggta agcagtgaaa atgcaacgcc ttcggtattg
     4561 gaaccgaact tccttatgct tgctgtcatt aaacctaatc ctgcagcata aacaattttt
     4621 cctttacgtg taacggggct ggtagtgtag tcggtcgcca tatatatgga acccaaaatc
     4681 aatcctcccg caagtatgct gtagagagga tcctgaccga atgtaaaact caacaaggca
     4741 accgttccga tatatatcgc cgggatttcc catgatatta cttttcttat cataagatat
     4801 attccgcctg ctataagtgt taccgctgcg gtttcaccca aactccccgg tgtttttccc
     4861 agaaacaggt caagatagga tggtaacatc tctcctttgg gtatggccaa aggtgttgcc
     4921 gccgtcactc cgtcggtcaa aggagccaca tataccgtca ttaaagagga aaaagcggta
     4981 gtcagaaaaa ttcttgcggc agcagccgga ttgaatatgt tctgcccgat tcctccgaaa
     5041 agcattttga ttaccactat ggcgataaat gcgcctataa cgcttaaaat aatcgaagac
     5101 ttcaaaggag gcatattcat tgccagaatc agaccggtaa caacacagga aaggtcggtt
     5161 tcggtatgtc tcttttttgt tatcagattg aaaagatact caaacaaaac acatgatgaa
     5221 actgaagcaa ctatgataag cgccgaaata taggaaaaat agtataccga taatacggct
     5281 gtcggcatta atgctattat aacatccagc attatccttg atgtggtggc tttgtccttt
     5341 atatgcggtg aagacgatac tattaattta tccatttttc accttcgctt tcctcacaag
     5401 atcttttcct acacggaaag actgtacaag atgccgcttt gccggacaga catatgaaca
     5461 ggatccgcat tcaatgcagt tcattatatt atatttttcc aattcctcgg tcattttggc
     5521 caaggcatat ctgttcattg tcaggggcat aagtcccatg ggacatgcct ctacgcatcg
     5581 gccgcatctt atgcatgccg attcttccat aaaatccaaa tcttccgggg tcataaacag
     5641 aaatgcgttg ctctgtttaa taaccggcga ctcatagctc tccgcagttg ttcccatcat
     5701 gggaccgccg caaagcacct ttaccgtttt ttctttatca ccttacagta ttcaaatata
     5761 tcttttatgt gtgttcctat aacgacctga taattccctc tattcttaac tccgctgcct
     5821 tcgacggtga ttcttttgga aaccagaggc attcctgtct tgcagtactt gtataatgcc
     5881 agcacgctgg ttacattcat gaccaccgtt cctatgtcaa tgggcagacc gttcatcggt
     5941 acttcttttc cggttaaggc ataaatgagc attttttccg caccttgcgg atattttgtc
     6001 ttaagcataa ccgtctgtat cccggaatgg tcttttgtaa gttccttgag cagggaaaat
     6061 gccgcaggct tattgtcttc cactgcaata tatgctttgg gaatggccag tttatctaaa
     6121 agaagcttaa ctccgtttac tatttcgcct ccttcttcta ttattgctct gtagtcacag
     6181 gtaatgtacg gttcacattc cacagcattt ataatgagag cttccggtat tctttccttt
     6241 gtgtccagtt tgacatgagc gggaaatccg gctcctccca aacccgtcaa tccgctgttt
     6301 cgaacagctt taagaaattt ttcctttgtc gatatatccg gttggtctat acccggtaaa
     6361 acttcctgct ttccgtcggt ctcaataact atgctttcac attttacgcc gttatgcagg
     6421 attatatctt ttatctgcac aacgttaccg gatacgctcg aatgaatcgg tgcggataca
     6481 aacttctccg aatctcccaa aacttgtcct acataaacat aatccccttt accgacaagc
     6541 actttgcagg gtgctccgat atgctgttgc atggggattt caacaagctt cgggattgga
     6601 agaatttccg taggaatctc cgaagttcct ttcctgtgag gtaaggcaac tccttttata
     6661 tttttgactt ttattatcat ccactccctc gccaattata atcattgata ttatacacct
     6721 ctacaggcag aaaaaaaagg acattgtatg cccttttctt tgattcctga tataatatcg
     6781 tttattccgt tgccaggaac aaaacatatt ccgctattgt atttgcatat tcttcatatg
     6841 tcattgacgg atcttccgtg aaggacatat agacctgcat tctctctgaa aggcatccct
     6901 ttatttcatt aagactcata ggaacttcct gctgttcggt atcatcggga actttgtaaa
     6961 gaacgcgtaa tgtaagatcg gtgacatctt cgccgtcaaa gacgaaactt acattgtcag
     7021 tagccatata cgctttggtt atctcttttg ctcctaccgc atttcttgca ttattgaaga
     7081 atatcatgaa ttcctcccat gcatcggatt cctgattggt ccccagaagt gccgggaagt
     7141 tcatcaaaag cgttccgtca atctccgaac cgataatcat attaacacct ttgtttgtta
     7201 ccgtcgtttc aaaaccggta aagtattcac aggaaaacag ataatggttc atctgtctta
     7261 tctcgtatgt ctggtacagt tgctcattcc tttcctgtac gggatctctt ccgatcattt
     7321 cattaaaagc actcagcctt gcattcagtg tcttctgtct gctgatgtta ttgaactgag
     7381 aaacggtagg cttcattatc ggttctccgc cgtacgtggc aatatcataa ggaaactctt
     7441 taatgacata tctgtcttct aaagcatttg cagtgttgat agacggtcta ggtcttttat
     7501 gatacggggt acatgaggta agccccatta caaatcctat tgcaataatt actgctatta
     7561 ctataactgt ttttcttctc atttatccct cccatgccct tgaatagcac tttttatgaa
     7621 ataattagta cctatattat acaacttaag attttgattg taaatcagtt ttcacaattg
     7681 catcatgttg tttatgtgat atttaatagg aaaattataa taaccattaa aatttactta
     7741 attatttcta ataccgtata aagattatat tctttttcac agacaggaaa aggacattat
     7801 ctgtcctttt cttctgcttt tgaaataata cctattcctg ttgtaaaacg tattccaaaa
     7861 ttgtatttgc gtattcttca taatccatta acggatcttc cgtaaaggac ataaatatct
     7921 gtattttctc caaaaaacac ttttttattt cattgagttt catgggaact tcctgctgtt
     7981 ttgtatcatc gggagcttta taaaggatac gcaatgtaag atcggtaaga tcgtcgccgt
     8041 caaacacgta actgacattg tcggtagcca tatattcctt cgttatttcc tttgcccctg
     8101 cggcttttct cgcttcttca aacagcacca tgaattccgt ccaggaatcg gattcctgct
     8161 ttgttcctaa aagtgccggg aaattcatta aaagcgttcc gttgatttcc gaacctacaa
     8221 gcatattaac gcctttgttc gtaaccgtcg tttcaaagcc ggtaaagtat tcacaggaaa
     8281 agagatagcg gttaatttgt ctgatttcat atgactggta tagttgttca tttctgtctt
     8341 gtccggaatc tctgccgacc atttcattga aagcattcag ccttgcattc agtgttttct
     8401 gcctgcttat gttgttaaac tgagcaacgg taggtttcat tatcggttct ccgccataag
     8461 tagcaacatt atattccgtt tccacaacta tatatctgtc ttccaaagca tttgcggtgt
     8521 tgatagaagg agaaatcctg atttcatgcg gggtacatga tgcaagcccc gttataaata
     8581 ctattgcaag aattactgct ataagcataa ctgtttttct tttcatattc acttttcctt
     8641 tcttaactgt ttctgagaaa taacaggcgc cggcattact tcttatgttt tgattgtaaa
     8701 tcggttttta taaatgcaca ataatgtaaa tgtgacattt aataggaata atataataaa
     8761 tgtcgagata ttcttgaaaa tatatgctat ttaatagact ttctgccgga atcttcataa
     8821 gaaagggaac aatatgatgc agtattgatg attgtcatgc ttttttcccg acaattctat
     8881 tataaattac acattactct cttaaaa
//
LOCUS       DAOPWC010000010         8215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_8715_length_8215_cov_3.691054, whole genome shotgun sequence.
ACCESSION   DAOPWC010000010 DAOPWC010000000
VERSION     DAOPWC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8715_length_8215_cov_3.691054"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKJ65_00485"
     CDS             <1..715
                     /locus_tag="PKJ65_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNZ40344.1"
                     /translation="EQFETLSTVVKGYNKNIIMFVDNCYGEFVKEQEPTQWGMDMAAG
                     SLIKNPGGGLCKSGGYIVGTKECIDNASARLYAPGLLKEVGATSNKRLMFQGLYMAPY
                     TVKESLKGAVFTALLFEKLGFKSYPKYDELREDIIQLVLFDKSEQLLQFCRGVQAGSP
                     IDSHVTPCPWEMPGYDDKVVMAAGTFVQGASIEFSADAPMRDPYVAYMQGGLTYTQVK
                     LGVLKACDIMIKKGMIDIR"
     gene            712..1971
                     /locus_tag="PKJ65_00490"
     CDS             712..1971
                     /locus_tag="PKJ65_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ40345.1"
                     /translation="MKKEREKRQSGKNTGAVIKILIIIAFVLFAILISRMAIRYYSSI
                     VKKDLAYEGRLTDQINISGLVLYDFDSYDTVSDHSLIRIAEEGSRAGVNHKIGVLADV
                     KNPLYEELCDINARITDIQQLYQINTGVLSKDIELIDMQIKDNFLSLSALLNENAMAD
                     AVERKNNIDSLYEYKTDLMSGNNTRQLQINALKSRQKELRQAMASDAIDIVCVNSGYV
                     TYNMYNGQHNHTYDAMYGMYPEDIKGSISCERDYTGNTVIKVATSRKFHIATILSSSD
                     AARIKDKKGLKIRINTGGLDFEVQVTKVITGNSSDGETVVFFETDNMLSSLVSLGRFD
                     GLLVIKEYSGLKVPVSAIKNYKYSSFQQTEIALVKGISVKFVKIDVLYSDGIYAIVQD
                     ADEDYSFKAYDYYILQPDKVSDGDIIN"
     gene            1983..2657
                     /locus_tag="PKJ65_00495"
     CDS             1983..2657
                     /locus_tag="PKJ65_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNZ40346.1"
                     /translation="MMESTNYVAENIYRIKKQLNNDDILIVAVSKTVSSETAALALEV
                     EGVELGENRVQEFLKKYEYFQGKAKIHLIGHLQRNKVKYVVGKTPLIHSVDSKELLDE
                     IQKRSAMSNVVTNVLLQVKIFDEETKYGVDESAVMDLINHNENNPNVLIKGLMTMAPL
                     TDDEQLISQIFGRLHDIYVDISKNTFYNTHMVYLSMGMTNDFMLAAKEGANILRIGSA
                     IFKDIP"
     gene            2701..3177
                     /locus_tag="PKJ65_00500"
     CDS             2701..3177
                     /locus_tag="PKJ65_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNZ40347.1"
                     /translation="MSVFDKFLTEFLGFEQAAEEKQTIAPVTRDGKTRSTVQINPIVE
                     RIDALDQIATKKVVPMERCTGTLNVMYPGSFEETKRVCDSLKANRAVIVNLEQVEKDL
                     AQRIVDFLSGTVYTLNGAIRKLSDKILVVAPSGYHIDDEKQEPAKVTDTKSRLYAN"
     gene            3187..3465
                     /locus_tag="PKJ65_00505"
     CDS             3187..3465
                     /locus_tag="PKJ65_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNZ40348.1"
                     /translation="MLKTVLTVIYYLLYALSFLVFIRVIASYFGGARFSKYYEVLVRL
                     TEPFLAPLRNFISWLAKGKPLMFDFSFIALYIIVMILQRIILVIQASL"
     gene            3651..4538
                     /locus_tag="PKJ65_00510"
     CDS             3651..4538
                     /locus_tag="PKJ65_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNZ40349.1"
                     /translation="MEYKDYYKILGVDKKDDKDTIKKAYRKLAKKFHPDNNPGNKRAE
                     ERFKEINEAYEVLYDDEKRKRYDTMGMNDFSSAGFDPSAYGQQYSYNYGGSSGFSRSN
                     DFSDFFNMFFGDLGMGADVFGQSSGRKSKAASRQFDSEASMKVSIEDALAQREMIVSI
                     NDKKVNLKIPEGIRSGQKIKMKGQGQGGGDLYIKIELQDTPNLTLENDDLIKTVDIYP
                     WQAYFGSVIQVEMPDNTLKVTIPKKIKGGARIRIKEKGYYKKDKTRGDLYIKVNIVNP
                     VRMSEKDEEIYKELSNRFG"
     gene            4554..4925
                     /locus_tag="PKJ65_00515"
     CDS             4554..4925
                     /locus_tag="PKJ65_00515"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNZ40350.1"
                     /translation="MKKADIILIVILICVFLIFLGLHLFNDSKKSDILVIQQDSKVIY
                     KESIHKDNEIQIKDRYGNITNIVTIKDGTAYMSFADCANKDCKHMGKLTPSSKKRIAC
                     LPNKVLVYLISTDSEVDGVTR"
     gene            4922..5431
                     /locus_tag="PKJ65_00520"
     CDS             4922..5431
                     /locus_tag="PKJ65_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNZ40351.1"
                     /translation="MNRTKKITISAIFAALAIALSVAESFIPFSAIAPGVKLGLANVV
                     PLTLLYVFGPAYAISVQAVRVSVAGLLRGNIVTFLFSFFGGTASITICSLIKRQKRVE
                     FSVIGLSVIGAVIHNAAQFFTALVILKNLNIFWYIAVLTIFAVIAGVLTGLISKYTIK
                     ALERYSNRF"
     gene            5486..6316
                     /gene="thyA"
                     /locus_tag="PKJ65_00525"
     CDS             5486..6316
                     /gene="thyA"
                     /locus_tag="PKJ65_00525"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNZ40352.1"
                     /translation="MSRADDIFKNMCRDIIDNGFWDTGLDVRPRWLDGTPAHSVKKFC
                     VVNRYDLSKEFPILTLRPTRFKGAIDEMLWIWQKKSNNIKDLNSKIWDAWADSEGSIG
                     KAYGYQLSVKHKYKEGMFDQVDRVLYDLKNNRASRRIITNIYVHADLHEMNLYPCAYS
                     MTFNVSGDVLNGILNQRSNDVLVANNWNVVQYAVLMHMMAQVSGLKAGELVHVIADAH
                     IYDRHIPLVEELLTREPYPAPTFWINPEITDFYDFTVEDFKLKNYQKNEQIKDIEVAV
                     "
     gene            6313..6813
                     /locus_tag="PKJ65_00530"
     CDS             6313..6813
                     /locus_tag="PKJ65_00530"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ40353.1"
                     /translation="MNIIVAVDEHFGIGNLGKIQWKIKEDMDFFRRSTLNKTVIMGRK
                     TLDSFKCGKPLKNRHNIVLTRDKCFKRENVTVVHSVKQALEKISDIDTGDVYIIGGQS
                     IYEAFLPYCDRAFVTKVHGVFDADTYFPDLDKSDEWKLIHQSDRQITQSGIHITFCEY
                     ERVRHE"
     gene            6806..8059
                     /locus_tag="PKJ65_00535"
     CDS             6806..8059
                     /locus_tag="PKJ65_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40354.1"
                     /translation="MSNGGKKSIRINKTYLILGVIYLIAFVVRIFIAGSITGHLTDMG
                     CFSYWGEQAYNEGLSEFYNGKLFCDYPPLYIYVLWVIEALRRAFGIVFAMPAHLILLK
                     LPTIIFDLLSAYFVFKIAKTRFSDKTSIILSACMAFNIMYILNSSAWGQIDSILTLLI
                     AITAYNIMNNKLHFATVAYIFAVLLKPQAFIFAPILIFAYIKSRNLKKVITSLLIGAG
                     IFVVVILPFAIGQKPLWIFGLYFGTMGQYAYATVNAYNLYALLGLNWIPDSEIMLLLP
                     YKIYGYIAIVGVCALTTAAFIKGHKKPGFTFATAGLLMTVVFTFATKMHERYIFPSIL
                     LVLAAFIFSNDRRFLYIHILQTLTCFANCAYILHLNYKNSYDFFSPFMAIVSAVTVFS
                     TLWYIKVYIQLYIMNKDNMPSKYLT"
BASE COUNT         2690 a         1408 c         1854 g         2263 t
ORIGIN      
        1 agagcagttt gaaacactca gcacagtggt taaaggatac aataaaaata ttattatgtt
       61 tgttgataac tgctacggag agtttgttaa ggagcaggag cctacccaat ggggaatgga
      121 catggcggca gggtcgctca taaaaaatcc cggaggggga ttgtgcaaaa gcgggggata
      181 tatagtcggt acaaaagagt gtatagataa cgcatctgca aggctgtatg ctcccggtct
      241 gttaaaggaa gtaggagcga caagcaataa aaggcttatg tttcaaggtc tgtatatggc
      301 tccttatacc gttaaagaga gtctgaaagg tgccgtcttc acggcattgc tatttgaaaa
      361 actgggattt aagagttatc cgaaatatga tgaattgagg gaagatataa tacagctggt
      421 actgtttgac aaaagcgaac agcttttaca gttttgccgg ggagtacagg ccggttcacc
      481 gatagattcg catgtaaccc cctgcccgtg ggaaatgccg ggatatgacg acaaggttgt
      541 aatggcggcg ggaacattcg tgcagggcgc ttccatagag ttttccgccg atgcacctat
      601 gagagatccc tatgtagcat atatgcaggg aggtcttacc tatacccagg tgaaactggg
      661 tgtacttaag gcatgtgata ttatgattaa aaagggtatg attgatatcc gatgaagaaa
      721 gaacgggaaa aaaggcaatc aggcaagaac acgggtgcgg ttattaagat attgataatc
      781 atagcttttg tgctgtttgc aatattgata agccgtatgg caatccgcta ttactcatcc
      841 atcgttaaaa aagatctggc ttacgaagga agacttacgg atcagataaa tatctccgga
      901 ctggtcttat atgacttcga ttcatatgat actgtctccg atcattcttt aataagaata
      961 gccgaagaag gaagcagagc gggtgttaac cataaaatag gggtgctggc tgatgtgaaa
     1021 aatcctttat acgaagagtt atgcgatatc aatgcccgta ttacggatat tcaacaactg
     1081 tatcagatta ataccggtgt tctttcaaag gatattgaac ttatagatat gcagattaaa
     1141 gacaattttc tgtcgctatc ggctcttctg aatgaaaacg caatggctga tgcagtggag
     1201 aggaagaata atattgactc tttgtatgaa tacaaaaccg accttatgag cggaaacaat
     1261 acaagacagt tgcagataaa tgcccttaaa agcaggcaga aagaattacg acaggccatg
     1321 gcaagcgatg ctatcgacat agtttgtgtc aattccggat atgtgactta caatatgtac
     1381 aacggacagc acaatcatac atatgatgcc atgtacggaa tgtatcccga agatataaaa
     1441 ggtagtatat catgtgagcg ggattataca ggtaacaccg tgataaaagt tgccacttca
     1501 agaaaattcc acatagcgac aatactttcc tcttccgatg ccgcaaggat aaaggacaaa
     1561 aaaggattga aaatacgcat aaataccggc gggctggatt ttgaagtgca agtgacgaaa
     1621 gtgataacgg gaaacagttc cgatggggag actgtagtat ttttcgaaac cgacaatatg
     1681 ctttcttctc tcgtttcatt gggcagattc gacggtttgc tggttatcaa agagtattcc
     1741 ggtcttaaag tgccggtaag tgcaataaag aattataaat actcctcttt ccaacagaca
     1801 gagattgcac tggtaaaagg catatcggtg aaatttgtaa agattgatgt tttatactcg
     1861 gacggtatat atgccatagt acaggatgcc gatgaggatt acagttttaa ggcatatgac
     1921 tattatattc ttcaaccgga caaagtgtcg gacggtgata taattaatta ataaaggcgg
     1981 atatgatgga atcaactaac tatgttgcag aaaacatata cagaataaag aaacagctga
     2041 acaatgacga cattctgata gtggcggtgt ccaagaccgt ttcctctgag actgcggctc
     2101 tggcgctgga agttgagggt gtggagctgg gagaaaacag agtgcaggag tttttgaaaa
     2161 agtatgaata ttttcagggt aaagcaaaaa tacacctgat aggtcacctt cagagaaaca
     2221 aagtaaaata tgttgtgggg aaaactcctc tgatacattc tgtggacagt aaagaactgc
     2281 ttgatgagat tcagaagcgg tcggcaatga gcaatgttgt tacaaatgta ctgctacagg
     2341 ttaagatttt tgacgaggaa accaagtacg gtgtggatga gtcggcagtt atggatttaa
     2401 taaatcacaa tgaaaataac cccaatgttt tgataaaagg tcttatgaca atggctcctt
     2461 tgaccgatga tgagcagcta atatctcaaa tattcggaag attacatgat atttatgttg
     2521 acataagtaa aaataccttt tacaatacac atatggtata tctttcaatg gggatgacca
     2581 atgattttat gttggccgct aaggagggcg ccaatatatt acgcatcggt tcagcaattt
     2641 ttaaggatat tccataaaat actaaagact atttgattgt acacaaaaga ggaggaaaac
     2701 atgagtgttt ttgacaagtt tctaaccgaa ttcttaggtt tcgaacaggc agcagaagaa
     2761 aaacaaacta tagcaccggt aacaagagat ggtaaaacca gatctactgt tcagattaat
     2821 cctattgtgg agagaattga cgcattggat cagattgcca cgaaaaaagt agtgcccatg
     2881 gaacgttgca caggtacttt gaatgtaatg taccctggca gcttcgaaga gacaaagaga
     2941 gtctgcgatt cgcttaaagc aaacagagcg gtaatagtca atcttgaaca ggttgaaaag
     3001 gatctggcac agagaatagt ggactttttg agcggcacgg tatataccct taacggcgct
     3061 ataagaaaac tttcagacaa gatattagtc gtagctccta gcggctatca tatcgatgat
     3121 gaaaagcagg aaccggctaa agtaaccgac accaaatccc ggttatatgc caactaaaga
     3181 ggtaagatgc tcaagaccgt actgaccgtc atatattatc tgctatatgc tctgtcattt
     3241 cttgtgttta taagggtgat agcatcatat ttcggcggtg cacgattcag caaatactat
     3301 gaagtgcttg tacggctgac cgaacctttc ctggcacctt taagaaattt catatcatgg
     3361 ttggccaaag gaaaaccttt gatgtttgat ttttctttca ttgctttata cattatcgtt
     3421 atgattcttc agcgtatcat attagtgata caggcgagtc tgtaaaatcc tcatatttat
     3481 ataaataaaa taccatatgg agtatatggt actttcttcc gttcaaaaat catactttac
     3541 cccatgtcgt ctaattgcgg tatagaagag atagaatact gttgaaaaga ttgtataatg
     3601 gatatataat atagtcatag gtcagagaaa gtcagactga aaggaaaggt atggagtata
     3661 aagattatta taaaatcctc ggagtagata agaaagacga caaggataca ataaaaaaag
     3721 catacaggaa actggcgaag aaatttcatc cggacaacaa tcccggcaat aaaagagccg
     3781 aggagaggtt caaggaaata aatgaggcgt atgaggtgct ttacgatgat gaaaagagaa
     3841 agcgctatga caccatgggc atgaatgact tttcttccgc aggtttcgat ccgtcggcat
     3901 acggacaaca atactcatat aattacggcg gcagttcggg tttttcacgg tccaatgatt
     3961 tttccgattt ctttaatatg tttttcggcg atctgggtat gggagccgat gtattcgggc
     4021 agtcttcggg acgcaagtcc aaagccgcat cccgtcagtt tgacagtgaa gcctccatga
     4081 aagtttctat cgaggacgca ttggctcaaa gggaaatgat tgtttccata aatgataaaa
     4141 aggttaatct gaaaataccc gaaggaatac ggtcgggaca gaagataaag atgaaaggac
     4201 agggacaggg gggaggagac ctttatataa aaatagagct tcaggacaca cccaacctga
     4261 cactggaaaa tgatgacctt ataaaaacgg tggatatata tccctggcag gcatatttcg
     4321 gttcggtaat ccaggtggaa atgccggaca ataccctgaa ggttaccata cccaaaaaga
     4381 taaaaggcgg agccaggata aggataaagg aaaaaggcta ctataaaaag gataagaccc
     4441 gaggtgactt atatatcaaa gtaaatatag tcaatcctgt aagaatgtcc gaaaaggacg
     4501 aagaaatata taaagaactg tcaaacaggt tcggttaatg ataaaatacg catatgaaaa
     4561 aggcggacat cattctcata gtcattctta tatgcgtatt cttaatattc ttgggactgc
     4621 atcttttcaa tgattcaaaa aaaagcgata ttcttgtaat acagcaggat tcaaaggtta
     4681 tttacaaaga gtccatacac aaagacaatg agatacaaat aaaagaccga tacggtaata
     4741 taaccaatat cgtaactatt aaagacggta ccgcatatat gtcttttgcc gattgtgcaa
     4801 acaaggattg taagcatatg gggaaactta caccaagctc aaaaaagcga atagcctgtc
     4861 ttcccaataa agttcttgtt taccttatat caacggattc ggaagtggac ggtgtgacaa
     4921 gatgaacaga acaaagaaga taacgatatc cgcaatattc gcagctctgg caatagcctt
     4981 gagtgttgct gaaagcttta tacctttttc cgcaatagct ccgggcgtaa aattagggtt
     5041 ggccaatgtg gtacctttga cgcttcttta tgtgttcgga cccgcatatg ccatatcggt
     5101 acaggccgta agagtatcgg ttgcgggatt gcttcgggga aatattgtta cctttctgtt
     5161 cagtttcttc gggggaacgg cttccataac aatatgctca ttgattaaaa ggcagaagag
     5221 ggtggagttt tccgtaatag gtttgagtgt gataggtgca gtaatacata atgctgcaca
     5281 gttttttacc gcattggtca tcttaaagaa tctgaacata ttctggtata ttgccgttct
     5341 tacgatattt gcggtgatag cgggagtgct taccggcttg atttcaaagt atacaatcaa
     5401 ggcattggaa agatattcaa acagatttta attatccgca atttaattgt ataatactat
     5461 aagaacaaag aaaaaggagt tcaatatgag cagagccgat gatatattca aaaatatgtg
     5521 cagagatata attgataacg gattttggga taccggtctg gatgtgcgtc ccagatggtt
     5581 agacggaaca cctgctcatt ctgtaaagaa attctgtgtc gttaacagat atgatttaag
     5641 taaagagttt cccatattga cactgcgtcc cacacggttt aagggagcta ttgatgaaat
     5701 gctgtggata tggcagaaga agtccaataa tataaaagat ttgaacagta aaatatggga
     5761 tgcctgggcg gacagcgaag gctcaatagg taaggcatac ggataccagt tgtcggttaa
     5821 gcataaatac aaagaaggaa tgtttgacca ggtggacagg gttttgtatg atttgaagaa
     5881 taacagagca agcagaagga taattaccaa tatatatgtt cacgccgacc ttcatgaaat
     5941 gaatctgtat ccctgtgcat acagtatgac tttcaatgtg tcgggtgatg tgcttaacgg
     6001 tatactgaat cagcgttcca atgatgtgct ggttgcaaac aactggaatg tcgtacagta
     6061 cgccgtactt atgcatatga tggctcaggt ttcgggctta aaggcgggag aactggttca
     6121 tgtaatagcc gatgctcata tttatgaccg acatattccc ctggtggagg agttgttaac
     6181 aagagagcca taccccgcac cgacattttg gataaacccg gagataacgg atttttatga
     6241 ttttaccgtg gaggacttta aactgaaaaa ctatcagaag aatgagcaga taaaagatat
     6301 tgaggttgcc gtatgaatat aattgttgcg gtggatgagc acttcggaat aggaaactta
     6361 ggcaaaatac agtggaagat aaaagaagat atggattttt tcaggaggag caccttgaac
     6421 aaaaccgtaa tcatgggcag gaaaacactg gattccttca agtgcggaaa acctctgaag
     6481 aacaggcata acatagtact tacaagagat aagtgcttta agagagagaa tgtaacggtt
     6541 gtgcacagcg taaagcaagc tttagaaaaa atatccgata tagataccgg tgatgtatac
     6601 ataataggcg gacagagcat atatgaggct tttttgccct attgcgatag agcatttgta
     6661 acaaaagtgc acggtgtatt cgatgccgat acatattttc ccgatctgga taaatcggat
     6721 gaatggaagt tgattcatca gtcggacaga cagattaccc aatcgggtat acatataact
     6781 ttttgcgagt atgaaagagt aaggcatgag taacggcggc aaaaaatcca tacggataaa
     6841 caaaacctat ctcatattag gtgtcatata cttaatagca tttgttgtcc gtatatttat
     6901 agcaggttcc ataaccgggc accttaccga tatgggttgt ttcagttact ggggcgaaca
     6961 ggcatacaac gaagggctgt cggagtttta taacggcaaa cttttctgtg actatccccc
     7021 tctttatatt tatgtgctgt gggtaataga agcattaaga agagcgttcg gtatagtatt
     7081 tgcaatgccg gcacatctga tattgctcaa acttcccacc ataatattcg atttgttatc
     7141 cgcatatttt gtatttaaga tagctaagac aagattttcc gataaaacat caataattct
     7201 ttcggcctgc atggcattca atattatgta tattctcaac tcttcggctt ggggtcagat
     7261 tgacagcata ctgacattgc ttatcgcaat tactgcatac aatatcatga ataacaaact
     7321 gcattttgca acggtggcat atatatttgc ggtgttactg aaacctcagg cattcatatt
     7381 tgctcccata ctgattttcg catatataaa aagcagaaat cttaagaaag ttataacttc
     7441 tcttttgata ggagcgggca tatttgttgt tgtaatactg ccctttgcga taggtcagaa
     7501 gcctttatgg atattcggat tgtatttcgg taccatgggg cagtatgcgt atgcaacggt
     7561 taatgcatat aacctgtatg cattgttggg acttaactgg ataccggaca gtgagataat
     7621 gttgctctta ccatacaaaa tttacggcta tatcgcaata gtgggagtgt gcgcattgac
     7681 aacggcggct ttcataaaag ggcataaaaa accgggattt acctttgcca cagcgggact
     7741 gcttatgacg gtggtgttta catttgcaac caagatgcat gagcgatata ttttcccttc
     7801 catccttctg gtacttgccg cctttatatt ctccaatgac agaagatttc tgtatataca
     7861 tattttgcag acactgacct gctttgccaa ctgtgcatat atactgcacc tgaattacaa
     7921 aaacagttat gactttttct cgccgtttat ggctatagta tcagccgtaa cggtattttc
     7981 tacattatgg tacattaaag tatatataca gctatatatt atgaataaag ataatatgcc
     8041 ttcaaagtat ttgacataac gggcatattg agtgaaaata tatgcgaaag ataaatattg
     8101 tttggacatg aggacaaata aatgaaacac aaaagacaaa tcatcaattc aattaaaaaa
     8161 gcaatagttg tagtaccgat aatatcagta tcagtactac aactattgct ttttt
//
LOCUS       DAOPWC010000011         8018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9003_length_8018_cov_3.635313, whole genome shotgun sequence.
ACCESSION   DAOPWC010000011 DAOPWC010000000
VERSION     DAOPWC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9003_length_8018_cov_3.635313"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="PKJ65_00540"
     CDS             <1..1403
                     /locus_tag="PKJ65_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40355.1"
                     /translation="ILTSNDSAIIDASKITVANGKLNFPIGAIGSKEGTVTLTAVGQN
                     ISSSIQITVKTPAAVKTLTITGVEGTVFVNQWVPLVVTAKDQYGNPTTLDTLDPTKLM
                     MFSTNTTVVPNTNIQFNGATKKIEIKGNASGSATLHISYDGITQGTLDVQVLEAPVPT
                     YITNVTMPIYYQKNSTIKMTADMLKIADQYEKEIALTPAHSVIVTSSDISGTKFTEGT
                     MTGAGYTMSAPATSGSYTLTFTLKKGASTVNTYDMTVLVVDDDKIVSYRIEPISTMYG
                     GATDTVTDTGHWKPIKIVGLLANGKSVNLAYTGTLPTGIDQITCTNDSGANNFVIEDV
                     SGVKTLKAQNVTQTMTTTIKLWKTGQEITSLNVTASGAAPTLTHLAATEPNVTKALSG
                     GVFNITAYVDAKDQYGVPMAFASITGSKTFMSSKTSVAVVNSTGIVTPVATGSCQITI
                     YFAGADKTLIINLTVN"
     gene            1543..2451
                     /locus_tag="PKJ65_00545"
     CDS             1543..2451
                     /locus_tag="PKJ65_00545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40356.1"
                     /translation="MEFRTLKESEFKEWTAFCGCIFPVGEAYFIRHFMNDPYRDINGI
                     FIAKDPTGIVSSVRVFTRYLRIKDNVIKVGGIGEVCTKPSHRGQGLSTKLLTMAIDYM
                     EKQDMPLSRLSTGNTHHYEKLGWFSLRFCHKVYRLRKSVLPEHYSVVQSSPVDMDAVK
                     SLFEEHAYKLCPVYVRNDEYYDKWVRSELINTYFLIKDRKVVSYADCVFKKGQGVEVR
                     EYIGGDEMYMMAPFFYAICKAEGWPKICGVSAPFGKDKESFRLYNSGQMFRINAPFEI
                     DGKIISCEQDLLPYLSELPYLPVDGY"
     gene            complement(2452..3696)
                     /locus_tag="PKJ65_00550"
     CDS             complement(2452..3696)
                     /locus_tag="PKJ65_00550"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNZ40357.1"
                     /translation="MTYIDRLGIKALKAKEQLIQIDTEKKNAALRLMAKNLLKHKEAI
                     KKANTDDVINAKEKGTKEALIDRLILNDARIESMAKGLMEISQFPEPIGNVLYERVLP
                     KGMKLKKISAPMGVIGIIYESRPNVTADAAALCFKAGSAVILRGGSEAINSNKAIVAA
                     LKDALEEIGINKDSIQLVEDTRRDTATQMMKADNYIDLLIPRGGAGLIASVIQNATVP
                     VIETGTGNCHLYIDSIFNEKFALDITVNAKCSRPGVCNAIETLLVHKDVYKDILPKIE
                     EELSKYKVQLRACEQSVKVLKNAIPATEADWSEEFLDYILAVKVVNDIEEAIEHINRY
                     GTKHSEAIVSDIEENRNKFTKNVDAACVYANTSTRFTDGGEFGLGAEMGISTQKLHAR
                     GPMGANEITTYKYIVEGNGQVR"
     gene            complement(3708..4511)
                     /gene="proB"
                     /locus_tag="PKJ65_00555"
     CDS             complement(3708..4511)
                     /gene="proB"
                     /locus_tag="PKJ65_00555"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ40358.1"
                     /translation="MRTTLQQADKIVIKVGTNTLTHANGKMNLRSMELLVREIAELVN
                     AGRKVILVTSGAVGVGLGKLNLMKKPKNLAARQVLASVGQCELMHIYSKLFSEYSITV
                     GQILLTRDVTENPVKAENAYNTFKGLMDMGIVPIVNENDAISTDQLAIVDSFGDNDTL
                     SAMVAKLIKADLLIILSNIDGLYDKDPNINEDAKIVDTVTEINKEIESYASDLTSSLG
                     RGGMKTKLTAAKTCLENDITMAIINGDNMKNISALLDGDKIGTLFTKRL"
     gene            complement(4513..5229)
                     /locus_tag="PKJ65_00560"
     CDS             complement(4513..5229)
                     /locus_tag="PKJ65_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNZ40359.1"
                     /translation="MYQPRDYRKSFTDRLKWYNVCVEETDLMIGTDEDCSELILKTVR
                     TLRKEIYAAIRDNGEFLTSLVPVEYKGEGVVKAMVEAAKLAKVGPFAAVAGAINDMLA
                     EKLMDKSKQIFIENGGDIYIKSDRDRLIGIYAGNSVLSGKFAIEIEKDLFPVGVCTSS
                     ATVGHSFSMGKADAAVVISKTCAVADAFATAFCNRIKKTEDIAPALEWVNGFDEVIGA
                     VAVMNDKIGAIGNIKLVKWN"
     gene            complement(5237..5641)
                     /locus_tag="PKJ65_00565"
     CDS             complement(5237..5641)
                     /locus_tag="PKJ65_00565"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ40360.1"
                     /translation="MSKLKLKLSFDKDCVTKPVIYELVKKYDVMTNILSANIEYGNRG
                     NMILEIKGKEENLSLTLEYFRSLNIKYEIYAEAILWDESKCINCGSCTAVCPTQALTM
                     GKDDLLHFDKDKCVVCQLCINACPIRIISVEL"
     gene            complement(5641..6789)
                     /locus_tag="PKJ65_00570"
     CDS             complement(5641..6789)
                     /locus_tag="PKJ65_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNZ40361.1"
                     /translation="MRTIAEINEKIKKGKAVVVTAEEVIDIVQEKGYKKAYETIDVVT
                     TGTFGPMCSSGVFINFGHSNPPIRMQSIMLNDVCAYGGLAAVDTYIGATEVSQKRGME
                     YGGGHVIEDLIAGKDIKLHALSSGTDCYPRKEILTTINKDNINQAYMFNPRNAYQNYP
                     AATNSTNKTIYTYMGTLLPNYGNITYSTSGELSPLLNDPYLRTIGVGTRIFLGGGVGY
                     VAWQGTQHKVDVERSANGIPLVHAATLAVIGDIKGMSTEYVRGAVFDKYGVTLFVGVG
                     VPIPVLDEEMLKYVSVKNSEIYTKLVDKSGNVNIDKKYNYAELRSGTVKFNGKAVHTA
                     PLSSLDVARKIACTLKEWINRGTFELTEKVADLPCESVVHPLEIREIK"
     gene            6965..>8018
                     /locus_tag="PKJ65_00575"
     CDS             6965..>8018
                     /locus_tag="PKJ65_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ40362.1"
                     /translation="MGFENRLTEGNVAKQLIKFSLPLLLSNIVQALYSVADMFIVGWL
                     SGTYTISGVNIGAQVTMIVTNFVIGLAIGGTVLIAQYIGAKKHEEVNKTIGTLFSVMG
                     LSAIFFSIFMTFFVDVMLKVLNTPEESYSMAKDYVLICMWGIIFVFGYNAISAILRGM
                     GDSKRPLMFVGIACVINVVLDILFVGPLGMKAAGAALATIISQAISMILAVIYLKRSG
                     FIFEFRRENFKIHKDKLKLLLKVGIPSSAQSVVVGISFLVLTSMINDIGGVTASAAVG
                     IVGKFNSFAILPAAAMSASVSAMAAQNIGAGLYDRASKTLWTAIAISFSISIVIFTFV
                     QVFPESILLAFKAEPDV"
BASE COUNT         2148 a         1816 c         1574 g         2480 t
ORIGIN      
        1 taatactgac atccaacgat tccgccataa tcgacgcttc caagataacc gtcgcaaacg
       61 gaaaactgaa tttccctata ggagcaatag gctctaagga aggtaccgtt acattaacgg
      121 ctgtaggaca gaacatatct tcttcaatac agattaccgt taagacacct gccgccgtaa
      181 agactcttac aataacagga gtggagggaa cggtatttgt aaatcagtgg gtacctcttg
      241 tagttaccgc aaaagatcag tacggcaatc ctacaacgtt ggatactctt gaccctacta
      301 aactgatgat gttctccacc aatacgacgg ttgtacccaa taccaacata cagttcaacg
      361 gtgcaaccaa gaagattgag ataaaaggaa acgcatcagg ctcggcaacc ctgcatattt
      421 cctatgacgg tattactcag ggcactttgg atgtacaggt gctggaagct ccggttccca
      481 catatattac caatgtaacc atgcccattt attatcagaa aaactccact atcaaaatga
      541 cagcggatat gctgaagata gccgaccaat atgaaaaaga aatagctctt actccggcac
      601 attcggttat tgtgacttct tcggatattt ccggtactaa gttcaccgaa ggtaccatga
      661 cgggagcggg atatacaatg agtgcacctg caacttcagg ctcatacaca ttgactttca
      721 ctctcaaaaa aggagcttcg accgttaata catacgatat gacggtattg gtagtggatg
      781 atgataaaat agtcagttat agaatagagc cgatatctac catgtacggc ggagctaccg
      841 atacggttac cgatacgggc cactggaaac ctataaagat agtgggacta ttagctaacg
      901 gaaaatcggt taaccttgca tacacgggta cccttccgac gggaatagat cagataacct
      961 gcacaaacga ttccggagcc aataacttcg taatcgagga tgtttcggga gtcaagactc
     1021 tgaaagctca gaatgtaact cagaccatga caactacaat caaactgtgg aaaaccggac
     1081 aggaaattac cagtctgaat gtaaccgctt cgggtgccgc tcctacactt acacatttag
     1141 ctgcaaccga gcccaatgtt accaaagcct tgtcgggcgg agttttcaac attactgcgt
     1201 atgtagatgc aaaagaccaa tacggagtac ccatggcctt cgcatccatc acgggcagca
     1261 aaacatttat gagcagtaaa acatcggttg ccgtagttaa ttcaaccgga atagttactc
     1321 cggtagccac aggctcctgc cagataacaa tatatttcgc cggtgccgat aaaacattga
     1381 ttatcaacct gaccgttaac tgataagcag ataatttaca cagacagact gcacgggcaa
     1441 ccgtgcagtt tgtttttcta ttgtttttcc gttaaatttc cactatgatt ctgttagcgg
     1501 tgaatatata ggtatgcaca aaaaccgtta agtgaggtaa caatggaatt ccgaacatta
     1561 aaagaaagtg aatttaaaga gtggactgct ttctgcggtt gcatttttcc tgtcggtgag
     1621 gcatatttta ttcgtcactt catgaatgac ccttacagag atataaacgg gatttttatt
     1681 gccaaagacc caaccggaat agtaagttcg gtaagggtgt ttaccagata cttgaggata
     1741 aaggacaatg taataaaggt cggaggaata ggagaggtat gcactaaacc ttctcacaga
     1801 ggacaaggat taagcacaaa actcttaact atggccatag attatatgga gaagcaggac
     1861 atgccgcttt ccaggctttc tacaggcaac acccatcatt acgaaaaatt aggctggttt
     1921 tctcttcgct tctgtcacaa agtatacaga ttacgaaagt cggtattacc ggaacactat
     1981 tccgttgtgc aatcctcacc tgttgatatg gacgcagtta aaagtctttt tgaagaacat
     2041 gcttacaaat tatgccccgt atatgtgcgc aatgacgagt attacgataa atgggtgcga
     2101 agtgaactta taaatacata ttttcttata aaagaccgta aggttgtatc ctatgccgac
     2161 tgtgttttca aaaaaggaca aggcgtggaa gtaagggagt atataggcgg agatgaaatg
     2221 tatatgatgg cacccttttt ctatgcaata tgtaaggcgg aaggctggcc taaaatatgc
     2281 ggtgtatccg ctccttttgg taaagacaaa gaatctttca ggttatataa cagcgggcag
     2341 atgtttcgga taaacgcacc gtttgaaata gacgggaaaa tcataagctg tgagcaggat
     2401 ttattgccct atttatccga gttgccctat ttacccgtag acggctattg attatcttac
     2461 ctgaccgttg ccttctacta tatatttata tgtggttatt tcgttggctc ccataggtcc
     2521 tcttgcgtgg agtttctggg tacttatacc catttccgct cctaatccga actcgcctcc
     2581 gtcggtaaat ctggtggagg tatttgcata tacgcaagcg gcatcgacat tcttggtaaa
     2641 cttgttcctg ttctcttcaa tatcggaaac tatggcttcg gagtgctttg taccgtacct
     2701 atttatatgc tctatggctt cctctatgtc attcaccact tttacggcaa gaatatagtc
     2761 caagaactcc tcagaccagt cagcttcggt tgcaggtatt gcatttttta atacctttac
     2821 tgattgctca caggctctta actgcacttt gtattttgaa agctcttctt ctattttcgg
     2881 cagtatatct ttatacacat ctttatgcac caacagtgtt tcaatggcat tgcacactcc
     2941 cggacgggaa cacttggcat tcaccgttat atccagtgca aacttttcgt taaaaatgct
     3001 gtcgatatag aggtggcagt taccggtacc cgtttctatt accggtacgg tggcattctg
     3061 tattaccgaa gctatgaggc cggctccccc tctgggtatc aaaaggtcta tatagttgtc
     3121 ggctttcatc atttgagtag ccgtatctct tcttgtatct tccaccagct gtatactgtc
     3181 tttatttatc ccgatctctt ccaaagcgtc ttttaaagcc gctacaatag ccttattgga
     3241 gtttattgcc tctgagccgc ctctcaatat gaccgcgctt ccggctttaa aacaaagagc
     3301 tgcggcgtcc gccgtgacat tgggacgcga ttcatatatt atgcctataa cccccatggg
     3361 ggcggaaatt ttcttaagtt tcataccttt aggtaatact ctttcataca gaacattgcc
     3421 tatcggttca ggaaattgtg atatctccat aagtcctttt gccatgcttt caattctggc
     3481 atcattaagg ataagtctgt ctatcaatgc ttctttggtg cctttttctt tcgcattgat
     3541 tacatcatca gtatttgcct ttttgatagc ttctttgtgc tttaagagat tttttgccat
     3601 cagtcgtaaa gcggcatttt tcttttcggt atctatttgt ataagctgtt cttttgcttt
     3661 taatgctttt attcccagcc ggtctatata tgtcattact cctcccgtta taaccttttt
     3721 gtaaataaag tacctatttt atctccgtcc agcaaagcgg agatattttt catgttatct
     3781 ccgttgataa tagccatggt tatatcattc tccagacagg tcttggctgc cgtaagtttt
     3841 gtcttcatac cgcctctgcc cagagagctt gtgagatcgg aagcatagga ctctatttct
     3901 ttgttaatct cggtaaccgt gtccactatc tttgcatctt cgtttatgtt cggatcttta
     3961 tcatacagtc cgtcaatatt ggaaagtatt atgagaaggt cggctttaat aagttttgcc
     4021 accattgccg ataacgtgtc gttatcaccg aaggaatcca ctattgccag ctgatcggta
     4081 gagatggcat cgttttcatt aactataggt acaatgccca tatccataag acctttgaaa
     4141 gtattgtacg cattttccgc cttcacggga ttttccgtta catcacgggt aagaagaatc
     4201 tgtcctacag taatggaata ttccgaaaaa agcttggaat atatgtgcat aagctcacac
     4261 tgaccgacag aagctaaaac ctgcctggca gcaagattct tgggtttttt cataaggttt
     4321 aacttaccca atcccactcc tacagcacct gaagtgacta atataacttt tctgcccgca
     4381 ttgaccaact cggcaatctc ccggaccaaa agttccatac tcctcagatt catctttccg
     4441 ttggcgtgag taagcgtatt ggttcccact ttaattacta ttttgtccgc ttgttgtaat
     4501 gttgttctca tatcagttcc atttcaccag tttaatattt cctattgcac ctattttatc
     4561 attcattacc gcaacagccc ctatgacctc atcaaacccg tttacccatt ccaatgccgg
     4621 ggcaatatct tcggtctttt ttatcctgtt acaaaatgcc gttgcaaagg catcggccac
     4681 tgcacaggtt ttacttataa ctactgccgc atcggccttt cccatactga aggaatgtcc
     4741 cacagttgcg gaagaggtac ataccccgac gggaaaaagg tctttttcta tctctattgc
     4801 aaacttaccg gataaaaccg agttacccgc atatatgcct atcaatctgt cccggtcgga
     4861 tttaatgtat atgtctccgc cgttttctat aaatatctgc ttactcttat ccataagttt
     4921 ttctgcaagc atgtcgttta tcgctcccgc caccgccgca aaaggtccca ctttagcaag
     4981 tttagccgcc tcaaccatgg cttttacaac accttcacct ttgtattcaa cgggcaccag
     5041 cgatgtcaaa aactctccgt tgtctcggat agcggcatat atttcttttc tcaaagttct
     5101 tacagtcttc aatatcaatt ccgaacagtc ttcatcggta cctatcatga ggtctgtttc
     5161 ttccacacag acattgtacc atttcaaccg gtcggtaaag gactttctgt agtctctcgg
     5221 ctgatacatc acacctctac aattccaccg atattatacg tatgggacat gcgttgatgc
     5281 aaagctggca gactacacat ttatccttgt caaaatgcag aagatcatcc ttgcccattg
     5341 taagcgcctg cgtcgggcac accgctgtgc agcttccgca gtttatgcat ttcgattcat
     5401 cccacaatat tgcttcggca tatatctcat atttaatatt caaagacctg aaatactcta
     5461 aagtaaggct cagattctcc tctttgcctt ttatctccag aatcatattg cctctgttac
     5521 cgtattctat attggcactt aatatattgg tcattacatc atatttcttg accagttcgt
     5581 atattaccgg ttttgtgacg cagtctttat caaatgaaag tttaagttta agtttgctca
     5641 ttattttatc tcccttattt ccagcggatg aacaacactt tcacaaggaa ggtccgctac
     5701 tttttccgtc aactcaaaag tacctctgtt tatccattcc tttaatgtac aggcaatctt
     5761 acgtgccaca tccaaagagg ataaaggtgc cgtatggaca gcctttccgt tgaactttac
     5821 agtcccgctt cgcaattcgg cataattgta tttcttatct atgttgacat tacccgattt
     5881 atctacaagt tttgtatata tctcggaatt tttaaccgat acatatttaa gcatctcttc
     5941 atccagtacg ggaatgggaa ctcctactcc tacaaaaagc gttactccgt atttgtcgaa
     6001 caccgctcct cttacatatt ccgtactcat tccctttata tcccctatca ccgcaagagt
     6061 ggccgcatgt actaaaggaa taccgtttgc acttcgttcc acatcaacct tgtgctgtgt
     6121 accctgccat gccacatatc ctacaccgcc tcccaagaat atcctggttc ccactcctat
     6181 ggtccgaaga tagggatcat ttagcagcgg actcaattca cccgacgtac tgtatgttat
     6241 gttgccgtag ttgggaagaa gcgttcccat atatgtatat atggtcttat tggtggagtt
     6301 ggtagccgcc ggataattct ggtatgcgtt tctgggattg aacatgtatg cctgatttat
     6361 gttatccttg ttgatggttg tgagtatttc ttttctcgga taacaatcgg ttccggaaga
     6421 cagcgcatgc aatttaatgt cttttcccgc tatcaaatct tctattacat gccctccgcc
     6481 gtattccatg cctcttttct gcgacacttc ggtagcccct atatatgtat ccacagccgc
     6541 aagaccgccg tatgcacaca catcattaag cataatgctc tgcattcgta tgggagggtt
     6601 ggaatgaccg aagtttatga acacccccga tgagcacatg ggaccgaatg ttccggtagt
     6661 caccacatca attgtttcat aagctttttt atagcccttc tcttggacta tgtctattac
     6721 ttcctccgcc gttaccacaa cggccttgcc ttttttaatc ttttcattta tctcggcaat
     6781 ggttctcata atacctttta tgcgacatgt tttttctttg tcacatactc cttccctgct
     6841 gattagggta attatatatt taaacacact ttttgtaaat gtaatacata aggctagata
     6901 acaaaaacct tgttaacaaa ggtatattgt gctaacatta caccattgca taaacggagg
     6961 cattatggga tttgaaaaca gattaaccga aggaaatgtg gcaaaacagc tgattaaatt
     7021 cagtcttccg ctgctgcttt ccaatattgt gcaggccttg tattccgttg cggatatgtt
     7081 tattgtggga tggttaagcg gtacatacac catatcggga gtaaacatag gcgcacaggt
     7141 caccatgata gtaaccaact ttgttatagg tctggcaatc ggaggtacgg tgcttatagc
     7201 gcagtatata ggggcaaaga agcacgagga agttaataaa accataggca ctctgttttc
     7261 ggtaatggga ctttcggcaa tcttcttttc aatcttcatg accttctttg ttgatgtaat
     7321 gttaaaagta ttgaataccc cggaagaatc ttattccatg gcaaaagatt atgtactcat
     7381 atgcatgtgg ggcatcatat ttgttttcgg ttataacgcg ataagtgcga tattaagagg
     7441 tatgggagat tccaagcgac ctttgatgtt tgtgggtata gcatgtgtaa taaatgttgt
     7501 gctggatatc ctctttgtag gtcctctggg catgaaagcg gccggtgccg cacttgccac
     7561 cataatctcc caggctatat ccatgatatt ggctgttatt tatcttaaaa gaagcggatt
     7621 catatttgag ttccgtcgtg aaaattttaa aatacacaaa gataaactga aactgctgct
     7681 gaaagtgggt attccttcca gtgcacaaag tgtcgttgtg ggtatttcct ttttggtgct
     7741 gacatccatg ataaatgata taggcggggt gacagcttcc gctgcagtgg gtatagtagg
     7801 taaattcaat tcctttgcca tacttcccgc cgcagccatg agtgcatcgg tttccgctat
     7861 ggctgctcag aatatagggg cggggctgta tgacagagca tccaagactc tctggactgc
     7921 tatagccata tctttttcca taagcattgt catattcact tttgtacaag tatttcccga
     7981 atccattctt ctggcattca aagcggaacc ggatgttg
//
LOCUS       DAOPWC010000012         7879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9203_length_7879_cov_3.841002, whole genome shotgun sequence.
ACCESSION   DAOPWC010000012 DAOPWC010000000
VERSION     DAOPWC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9203_length_7879_cov_3.841002"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PKJ65_00580"
     CDS             complement(<1..1234)
                     /locus_tag="PKJ65_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNZ40363.1"
                     /translation="MALGIGIDTGGTYTDAVIYDFDSGKVLCCAKALTTHEKLETGII
                     NALSRLNKQYFPKVEIVALSTTLATNACIENKGGRAKLLFFGVEQKVFDMVGKDYGLT
                     DKSDVYLCEKSGDFSGNNTQQPDWDKLFTQLDDWLNDAEGLGIVEVFAASNGAKLEKI
                     AKQLVEERYGIPAVCGHELFYELNSIQRGAGTLLNAKLIPIIKQFLESIKSALEKMHI
                     NAPVVVIRSDSTKMSEQYAETKPVETILSGPASSVTGGVNITEEKNCLIIDMGGTTTD
                     VSIVKNGIPLDADSGVRIGSWKTFVHGVFIDTFGLGGDSTIRVEHGKVVQLPQRMIPL
                     CVAATRWPLINKELEELIDSKKYHSYPLYEFLLLQKSVTGNNSHIIHNRYTKEESELI
                     NLLEKQPMPLEKAANASGK"
     gene            complement(1225..2481)
                     /locus_tag="PKJ65_00585"
     CDS             complement(1225..2481)
                     /locus_tag="PKJ65_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40364.1"
                     /translation="MITIAVIGLGNRGSGYMRWTKLLNGRKAKITALCDTDEYRLNTY
                     GDKYEVDKSNRYLNEVDFFKAGKLADAIYICTMDNDHYRHAMAAIECGYHIMLEKPVS
                     INIAECEEIRNKAKEKGVYVVVCHVMRYSPFFKKMNQLVKSGKYGKIISVEHTENIGY
                     FHFAHSYVRGNWRDSEKTTPMLMAKCCHDFDLIHWLMGDGCKSISSYGSLSYFKKENA
                     PEGCGDICLDCPKHIKNNCPYDAESLYITAPIWRFTFLKLLGDVITGKPKYNRKDKYD
                     SLRTGLFGRCVYKCDNNALDHQVVTMDYGDNRYVNLNVTAFSEKCYRHTILHMEKADI
                     HCDESKGRFIINRFGKGSKTVYAGVIRLAHISGDMALVNNFIKLLSGEPMETDDLTFI
                     DTTIVSHRNVMKAEQSRKEGGKVIWL"
     gene            complement(2478..2828)
                     /locus_tag="PKJ65_00590"
     CDS             complement(2478..2828)
                     /locus_tag="PKJ65_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNZ40365.1"
                     /translation="MNIQIFGKTKCFDTQKAQRYFKERKIPFRYINMSDKGMSKGELE
                     KVLRAVGGMENLLDYKTKDQDKLLRIKYAKEQDRTALLLENQHLIKTPVVRNGNKATA
                     GYEPGVWKEWEGNK"
     gene            2966..3589
                     /locus_tag="PKJ65_00595"
     CDS             2966..3589
                     /locus_tag="PKJ65_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40366.1"
                     /translation="MKAFFVKYRRLISLMLPFLLYIFYLIVCAIVKKSDNFFSTEMFL
                     DSYIPFIDFAVYGYISWVPLAAVSVIFLFFCPGDGYYRLIIAIAISVLICLIISLSYP
                     VRMDIPHFGDSAFLVAVLKNSGIASFPNIPSCILSNGFFLMIFYKKIHKRSKCLPIVL
                     LFGLILLAWIVCALLSKMTHISDLLIGILIGAVSSLSVWFIPFKQTH"
     gene            complement(3586..4419)
                     /locus_tag="PKJ65_00600"
     CDS             complement(3586..4419)
                     /locus_tag="PKJ65_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNZ40367.1"
                     /translation="MNRFNTIHLHTRYSDGLNDSHEYIEEAIRLGLKSIGFSDHAPVP
                     LKYDWAMKKEDLPRYFDELNYMRKVYGNKINIYIGLELDYLEGLDVKKYIDFDQLNLD
                     FVIGTVHYLYSDWIDGFASADGTQERVDKAIIEGFGGDGVAFYKKYYQTLRQMITEYN
                     PLMIAHFDIVEKRNIGKKWFDADTDEYKAHVEATLDVIKDSYIEVNTGAMAKDIDVMY
                     PSEYILRRCCAKNIPVSVNSDCHFTSALLFAYDKAIDRLKRAGYDCSYVFDGVMKKVK
                     F"
     gene            complement(4412..5347)
                     /locus_tag="PKJ65_00605"
     CDS             complement(4412..5347)
                     /locus_tag="PKJ65_00605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ40368.1"
                     /translation="MKKLYSLIIPLYNEEQVIHECIKRVTDVMKDKDFDYEVIFVNDG
                     SRDATEAIVKQYCAEDAHLKLISFSRNFGHQTAITAGMDHSAGDAVIVMDADLQDPPE
                     VVLQMIEKYEEGYDVVYAVRSKRKGETFFKKFTAKVFYRFLKSMCEVDIPVDTGDFRL
                     ISRQVCDVLKGLTERNRYVRGLVSWVGFKQTGIYYEREERFAGETKYPLKKMLKLSMD
                     GITSFSTKPLKLTKWIGLVTTASGFIYLLVVIIQRLMGIRLQNGWIPAVIAILIVGGI
                     QLIMSGINGEYIARIFDESKNRPLYIIREKVNVDE"
     gene            5573..5953
                     /locus_tag="PKJ65_00610"
     CDS             5573..5953
                     /locus_tag="PKJ65_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ40369.1"
                     /translation="MDLLYKKFGNRVREKRVMNNMSQEKLAEKCDVSTSYIGLVERGE
                     RKASLGVVLKIANALDVTPNALTMDSIKHKACNNKDKLVLMINDFDDAQLNTTFEIVN
                     QIHSYIKKGTRKAAYKDIDELLSD"
     gene            complement(5950..6144)
                     /locus_tag="PKJ65_00615"
     CDS             complement(5950..6144)
                     /locus_tag="PKJ65_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNZ40370.1"
                     /translation="MKTDIQLNDIVELKKAHPCGQNKWRILRTGADFRLECLGCGHKI
                     MLDRSDLFKKIKKVLKEDKD"
     gene            complement(6144..6779)
                     /locus_tag="PKJ65_00620"
     CDS             complement(6144..6779)
                     /locus_tag="PKJ65_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40371.1"
                     /translation="MKKVIIYILIIVILAAIPAGCNKKVEIVKPEHVSRGEVVDGYYV
                     NKDMGFKIKVPEDYYIATEEDLDTKFDIKQHVEDDELEMFITYLAFFSQKPIDDAQDN
                     SHIMISVEDASKYGYADKDTYIEYMSNSKADTYRNVKEAKITLSNQTNMWLGNRKFAN
                     RTITLDLHDLYIIFDMYAVVKNGYFLSIMIGAHNESDLEYLRGYLDTFSFE"
     gene            complement(6782..7198)
                     /locus_tag="PKJ65_00625"
     CDS             complement(6782..7198)
                     /locus_tag="PKJ65_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ40372.1"
                     /translation="MKSYRKELYFNIGTRRGFVNITTELMQCLKESGIREGLLLCNAM
                     HITASVFVNDDERGLHQDFEKWLEKLAPEKPYSQYAHNGFEDNADAHLKRTIMGREAV
                     IAVTDGKLDLGPWEQVFYGEFDGKRPKRVLVKIIGE"
     gene            complement(7283..>7879)
                     /locus_tag="PKJ65_00630"
     CDS             complement(7283..>7879)
                     /locus_tag="PKJ65_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40373.1"
                     /translation="CSNVVIENISGETTDDLIAINSATDVNLVFPFKQYVFPLIPSNI
                     CKDEESIEDRYITDVSVRNVYTSGVSGVALLSRHGNKIKNVCIENVIDTHTGPFIHPL
                     YPNATNLKLVYSYAGYFGETYTPGDISGVRISNAHSRFFKTTVFFGDVVHDVCIENVT
                     QQRSDGELITLHEQQDLKEIKVFCKNDTNNSGFTAKSI"
BASE COUNT         2114 a         1723 c         1372 g         2670 t
ORIGIN      
        1 ccttacccga agcatttgcg gctttttcca aaggcatggg ctgtttttcc agtaaattta
       61 taagttccga ttcttctttt gtatatctgt tgtgtatgat atgcgaatta ttaccggtta
      121 cacttttctg taaaagtaaa aactcgtaca aaggataact gtgatacttt tttgaatcaa
      181 taagttcttc caactcctta ttgataagcg gccatcttgt tgcggcaaca cacagaggaa
      241 tcatgcgttg aggcagttgc acaacctttc cgtgctccac ccttattgta ctgtctcccc
      301 ccaaaccgaa tgtgtctata aatactccgt ggacaaatgt cttccacgag cctatcctta
      361 ccccggaatc ggcatcaagc ggtataccgt tttttactat ggaaacatcg gtagtggttc
      421 ctcccatatc tatgataagg caattttttt cctccgttat attcaccccg cccgtaaccg
      481 atgaagcagg tccggataag atggtttcca caggctttgt ttcggcatat tgttcgctca
      541 ttttagtgct gtcacttcgt ataaccacaa cgggtgcatt aatgtgcatt ttttccaatg
      601 ccgactttat actctccaaa aactgtttga tgataggtat aagtttggca ttgagtaaag
      661 tgccagctcc tctttgaatg gagtttagtt cgtaaaagag ttcatgaccg cataccgcag
      721 gaattccata tctttcttcc accaactgct ttgcgatctt ttccagtttg gcgccgttac
      781 ttgcagcaaa tacttccact attcccaagc cttcggcatc gttcaaccag tcatccagtt
      841 gagtaaataa tttatcccag tccggttgct gcgtgttgtt gccggaaaaa tcaccgcttt
      901 tttcacatag atatacatca cttttgtcgg taagaccgta atccttgcct accatatcaa
      961 aaaccttttg ctccactccg aaaaacagca acttggctct tccgcctttg ttttctatac
     1021 aggcgttggt agccaaagtt gtggacagag ccactatttc cactttcgga aagtattgct
     1081 tatttaaacg ggataacgca tttattattc cggtctccag cttttcatga gtggtaagag
     1141 cttttgcaca gcataatact ttaccgctgt caaagtcata tattacggca tcggtatatg
     1201 taccgcccgt atctattcct attcctaaag ccatataact tttccgcctt cttttctcga
     1261 ctgctctgcc ttcattacat tgcgatgact gactatggtt gtatcgatga aggtaaggtc
     1321 gtcggtttcc atgggttcac cggataacag ttttataaaa ttattgacca gcgccatgtc
     1381 acccgatata tgtgcaaggc gtataactcc cgcatataca gttttacttc cctttccgaa
     1441 tctgttgatt atgaatctgc ctttggattc gtcgcagtga atatcggctt tttccatgtg
     1501 caggattgtg tgtctgtaac atttttcgga aaatgccgta acattcaggt tgacatatct
     1561 gttatcaccg taatccattg tcaccacctg atggtccaat gcattgttgt cacacttata
     1621 cacgcatctg ccgaacagac cggtccgcaa cgaatcatat ttatcttttc tgttgtattt
     1681 gggttttccg gttatgacat ctcctaaaag tttcaggaat gtaaaccgcc atatgggtgc
     1741 ggtaatatac aggctttccg catcatacgg gcagttgttt ttaatatgct tgggacaatc
     1801 caggcatatg tcgccgcaac cttccggtgc attttctttt ttaaagtaag aaaggctgcc
     1861 gtaggaagat atggatttgc aaccgtctcc catcaaccag tgtataaggt caaagtcatg
     1921 gcagcattta gccatcagca tgggtgtggt tttttccgaa tctctccagt tacctcttac
     1981 ataactgtga gcgaaatgga agtaccctat attctcggta tgttcaacag atattatctt
     2041 gccgtacttc ccgctcttta ccaattgatt catctttttg aagaacggcg aatatctcat
     2101 gacatggcat accacaacat atacaccttt ttcttttgct ttgttgcgta tctcttcaca
     2161 ctcggcaata tttatagaga cgggtttttc cagcattatg tgatatccgc attctattgc
     2221 cgccatggca tggcggtagt gatcgttatc cattgtgcat atatatatgg catcggccag
     2281 tttcccggct ttaaagaaat cgacttcatt cagatatctg ttacttttat ccacttcata
     2341 tttgtcgccg tatgtgttga gtctgtactc atcggtatcg cataatgcgg ttattttagc
     2401 ttttctgccg tttaaaagtt tggtccatct catataaccg ctgcctctgt tgcctaagcc
     2461 tatcacagct atggtaatca tttgttaccc tcccattctt tccatacacc cggttcatac
     2521 cccgctgtcg ccttattgcc gtttcgcacg acgggagttt ttatgaggtg ctgattttcc
     2581 aaaagcagtg ccgtcctgtc ctgctctttt gcatatttaa tcctcagaag tttatcctga
     2641 tcttttgttt tatagtctaa aagattctcc attccgccta ccgctcttaa aactttttcc
     2701 aactcgcctt tactcatacc tttatctgac atattgatat accggaaagg tatttttctt
     2761 tctttgaagt atcgttgggc tttttgggta tcaaagcatt tggtttttcc gaaaatctga
     2821 atattcatta ttccgcctct atatatccga ttgagtatat tttaacataa agcttgactg
     2881 tatataccga ttctttcgct tgtaagttta ccgataggat actataatgg gcatgagagt
     2941 tttttaagat acggaaagga aagttatgaa ggcttttttc gttaaataca gacggcttat
     3001 atcacttatg ttaccctttc ttttgtatat attttatttg atagtatgtg cgattgttaa
     3061 aaaaagcgat aacttctttt ccactgaaat gtttcttgac agctatattc cttttattga
     3121 ttttgccgtt tacggatata tatcctgggt gccgcttgca gccgtatccg ttattttcct
     3181 tttcttctgt ccgggcgacg gatattaccg gcttataata gcaattgcaa tatcggttct
     3241 tatttgcctg ataataagtc tttcctatcc ggtcagaatg gatattcccc atttcggcga
     3301 cagtgcattt cttgttgcag ttttgaaaaa cagcggtatt gcaagttttc ccaacatacc
     3361 ttcatgtata ctgtccaacg gctttttctt aatgattttc tacaagaaaa tacataaacg
     3421 ttcaaaatgc ttgccgatag ttttgctgtt cggattgata ttgctggcat ggattgtctg
     3481 tgctttgctt tccaagatga cacatatttc agatctgtta atcggaatac ttataggagc
     3541 ggtttcttct ttgtcggtct ggtttattcc ttttaaacaa acacactaaa atttaacttt
     3601 tttcataact ccgtcaaaga cataagaaca atcgtagccc gctcttttaa gccggtctat
     3661 cgctttatcg taagcaaaca gtaacgccga tgtaaaatgg cagtcggaat taaccgatac
     3721 gggtatgttt ttcgcacagc atcttctcaa gatatattcc gaaggataca tgacatcaat
     3781 gtcttttgcc atggcaccgg tgttgacttc gatataggaa tcttttatta catccaaagt
     3841 cgcttctaca tgagctttat attcatcggt atcggcatca aaccacttct tgcctatatt
     3901 ccttttttcc actatatcga aatgagcaat cattaacggg ttatattctg ttatcatttg
     3961 ccttaatgtc tgatagtatt ttttgtaaaa agcaaccccg tcaccgccga aaccttcaat
     4021 gatagcttta tccactcttt cctgtgtacc gtcggccgaa gcaaaaccgt ctatccagtc
     4081 ggaatagagg tagtggaccg tacctataac aaaatccaga ttaagttggt caaaatcaat
     4141 atactttttg acatccagcc cttccaaata gtccagctcc aatccgatat aaatatttat
     4201 cttgttcccg tacaccttcc tcatgtaatt taactcgtca aaatacctgg gcaggtcctc
     4261 ttttttcatt gcccaatcat atttcagcgg cacgggagca tggtcggaaa aacctatact
     4321 tttcagtccc aatcttattg cttcttctat atattcatgc gaatcattca acccgtcgga
     4381 ataacgggta tgtaaatgta ttgtattaaa tctattcatc cacgttaacc ttctctctta
     4441 taatatacaa aggtctgttc ttgctctcgt caaaaatcct ggcaatatac tcgccgttta
     4501 tacccgacat gataagctgt atgccgccca caatcagtat agcaattacg gcaggtatcc
     4561 atccgttctg caaccgtatt cccataagcc tttgtatgat tacaacgaga agataaataa
     4621 agcctgaagc cgttgtgact aaacctatcc attttgtcag tttaagaggt tttgtggaaa
     4681 acgacgttat accgtccatg gaaagtttta acatcttttt taaggggtat ttggtctctc
     4741 ccgcaaatcg ctcttctctt tcgtaatata ttcctgtctg tttgaatccc acccatgaaa
     4801 caagacctct tacatacctg tttctctcgg taagtccttt caatacatcg catacctgcc
     4861 tgcttatgag acggaagtcg ccggtgtcta ccggtatgtc cacttcacac atgcttttta
     4921 agaatctgta aaatactttg gcggtaaact tcttgaagaa ggtttcgcct tttcttttgg
     4981 aacgtacggc atataccaca tcatatcctt cctcatactt ttcaatcatc tgcaacacca
     5041 cttcgggagg atcctgaagg tcggcatcca taactataac ggcatcacct gccgaatggt
     5101 ccattccggc ggttatagcg gtctgatgcc cgaaattacg cgagaaagat ataagtttca
     5161 gatgagcatc ttcagcacag tattgcttaa caatagcttc tgttgcatcc ctgcttccgt
     5221 cattaacgaa aataacttca taatcaaagt ctttatcttt cattacgtcg gttactctct
     5281 ttatacattc atgtattacc tgttcttcgt tatacagcgg tattatgaga gaataaagtt
     5341 ttttcataca aaacacctca atgcaatatg atattcctat tatacatttt cccggctgat
     5401 aataacagat ttattgtaag gaatataata tataacaaaa tataaaatat aatgtaattt
     5461 ttcatatatt tttatgttgt atttatgtaa ttatatatta tatttatatt gactatttct
     5521 ttaataatgc tataatttga cataacaaaa aacagaaagg ggatatatgg taatggattt
     5581 actgtataaa aaattcggca acagagtccg tgagaaaaga gtcatgaaca acatgtctca
     5641 ggaaaagtta gcggaaaaat gtgatgtatc aacatcatac ataggtttgg tggaaagagg
     5701 tgaacgtaaa gcttctttgg gggttgtttt aaaaatagca aatgctttgg atgttacgcc
     5761 caatgctctc actatggatt caataaaaca taaagcatgt aacaacaaag acaaacttgt
     5821 cttaatgatt aacgattttg atgatgcgca gttaaacacc acttttgaaa ttgttaatca
     5881 gattcactct tacataaaga agggtacaag aaaagcagct tataaggata tagacgagtt
     5941 gctttccgat tagtccttat cttctttcaa aactttttta atctttttga aaaggtcact
     6001 tctgtccaac ataatcttgt gaccgcaacc gagacattcc agcctgaagt ccgcgccggt
     6061 tcttagaatg cgccatttgt tctggccgca ggggtgtgct ttcttgagtt ccactatatc
     6121 gtttaactga atgtccgttt tcattattcg aaagagaaag tatccagata acctcttaaa
     6181 tactccagat cggactcgtt gtgagcacct atcattatgg aaagaaagta tccgttcttt
     6241 acaacagcat acatatcaaa gattatgtaa aggtcgtgca aatccaaggt tatggttctg
     6301 tttgcaaatt tacggttacc caaccacata tttgtctgat tggataaggt tattttagct
     6361 tcttttacat ttctgtatgt atcggctttg ctgttggaca tatattcaat atatgtatct
     6421 ttatcagcat atccgtattt acttgcatct tcaacggata tcattatatg cgaattatct
     6481 tgtgcatcat ctataggctt ttgtgaaaag aaagcaagat aagttataaa catctccagt
     6541 tcatcgtctt ctacatgctg ctttatatca aatttagtat ccaggtcctc ttccgtagct
     6601 atgtaataat cttccggcac cttaatttta aatcccatat ctttgtttac ataatacccg
     6661 tcaactacct cacctcgcga cacatgctca ggcttaacaa tctctacctt tttattgcac
     6721 cccgccggta tggcggccag tattacgata atcaaaatat atattataac ttttttcata
     6781 atcattcccc tattatcttg acaagcactc ttttaggtct tttaccgtca aactcgccgt
     6841 aaaaaacctg ctcccacgga cctaaatcaa gttttccgtc tgttaccgct attaccgctt
     6901 ctctgcccat tatagttctt ttcagatgtg catcggcatt atcctcaaag ccgttgtggg
     6961 catattgcga atagggtttt tccggagcca gtttttccag ccacttttca aaatcctgat
     7021 gcaatcccct ctcgtcatcg ttaacaaata ccgaagcggt tatatgcatg gcattacata
     7081 aaagcaatcc ttcccttata ccgctttctt tcaggcactg cataagctcc gttgttatat
     7141 tcacaaaccc tcttcttgta ccgatattga aatatagctc ttttctgtaa cttttcataa
     7201 cactaatata ttttacaaaa agaaatgtga aaaaacaata ttttcaggtt gcttccccgc
     7261 cttttgccgt tttttaaatt attcatatgg attttgcagt aaaaccggaa ttattcgtat
     7321 cgtttttgca aaagaccttt atttctttca gatcctgctg ttcatgcaaa gtaataagct
     7381 ctccgtcgct gcgctgttgc gtaacatttt caatgcatac atcatggact acatcgccga
     7441 aaaataccgt agtcttgaaa aacctggaat gggcgttgct tattcttact cccgatatat
     7501 cgcccggagt atatgtttct ccgaagtatc ccgcatagga gtagacaagc tttaaattgg
     7561 ttgcattggg ataaagagga tgtatgaaag gtccggtatg ggtatctatg acattctcaa
     7621 tacaaacatt cttaatctta ttgccgtgtc gcgataacag agccactccg gacacaccgg
     7681 aagtgtatac atttctcact gatacatcag taatgtatct gtcctctatt gattcctcat
     7741 ctttgcatat attggacgga ataagaggaa agacatactg cttgaaagga aagacaagat
     7801 tgacatcggt ggcgctgtta atagcaataa ggtcatctgt tgtttccccg gatatattct
     7861 ctataacaac attgctaca
//
LOCUS       DAOPWC010000013         7763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9386_length_7763_cov_3.849896, whole genome shotgun sequence.
ACCESSION   DAOPWC010000013 DAOPWC010000000
VERSION     DAOPWC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_7763_cov_3.849896"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2700)
                     /locus_tag="PKJ65_00635"
     CDS             complement(133..2700)
                     /locus_tag="PKJ65_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ40374.1"
                     /translation="MKAIRLRTEYLQEPIGIDIVRPRFFWNCEGGIKQTAYRIICKRD
                     GETVWDSGKVLSDAMTHITYDGKPLCSRDIIDWSVKLWDEEGKEGEISSSKFEIGLLQ
                     PSDWKAQWITGNYIVCRKKRYPVDCFRKIFKCKDISKARLYITACGVYEALINGRRCG
                     TFVMAPGYTDYRKRIQYQTVDVTELLLSGENTLTVWLADGWYRGSCGSWGLKNQYGSV
                     TKLLVQLEITHTDNTVHTVISDDSWDWSNDGPIRFADNKDGEIYDANYTASYKGKAKL
                     TKNRVIPVASNNVGVSEQEHFTAKKIITPGGKTVLDFGQNMAGYVAFSLNAKARQKIT
                     LRFGEMMKSDGEFTQDNFQLVFGKHVTPLQQVIYTCKEGHNQYKTRFSIFGFQYVQVE
                     ADFDINPQDFEAIAVYSNLERTGFFDCSHPLVNGLVHATVWSAKSNHLDVPTDCPTRE
                     RHAWTGDAQIFFETASYLFDFAAFSKKYLQDVYDWQRKNGKLPHIAPDGGADFYMKPM
                     NGSVGWSDVGIIIPYQYYKIFGDRDILQRFYNGMANYARFMISRCGKTMPVFAKRIRL
                     SEGNRKYLVNTGQSYGEWAEPVDVCSFRWQDFATPHPEVSTAYTSYVLGLMAEIAEIS
                     GYEKDAREFEKYSKGCKNAYRELVSGGKYDLDTDRQAQLVRPLAFNLLNEEQTEFARK
                     RLIKALEYYRWRLGTGFLSTPMILSVLAEYDLEAAYRLLENEEIPGWLSMPLHGATTI
                     WENWEGPYNTQGAGLGSLNHYSKGAVCRWLFDTVCGIRVNGKNQFVVAPRPGGSLTHA
                     SASYNSIYGKVQSKWKKTDNKYEFTITVPANCEAVISLPDGSDFRQLAGTMIYTTEG"
     gene            complement(2723..3838)
                     /locus_tag="PKJ65_00640"
     CDS             complement(2723..3838)
                     /locus_tag="PKJ65_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ40375.1"
                     /translation="MKISKKDAYTWFKFFATLPEEEQLTAHQSEIVYAAFAQIEEVVD
                     KRNRELMASIGGLKTLQNRTYYVGNDDKFPNGCKSCLLGTGLSAVRKTNKCNLECKFC
                     YNYGELDDIPPVGEGMWEIGETKYYEKDIDLLLSVHKKPTGISYVYLEPFMEIEKYYP
                     VIKKFHDANIHQHMYTNGTLVTGQSVKILAGCGLDELRFNLGAANCSDKVIDNMRLAA
                     EHIRSVGIETPMTPEFIKGFFEKKNMILNTGIKFINCAELHLNPNNIANYYGENMYIS
                     RHGYISPVWSRELSLKLMKTSDDEKWDIVVHDCSNYTKFVRGLNLSAKEGKWFGANDY
                     ACEFPVIPYEYFLPILQDESFQFSDDQPLLAEYADRV"
     gene            complement(3870..5144)
                     /locus_tag="PKJ65_00645"
     CDS             complement(3870..5144)
                     /locus_tag="PKJ65_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40376.1"
                     /translation="MDPKESINVEFKQEVSKTFLKTVSAYANYNDGRIIFGIDDNGII
                     IGLDDAEAQLLRIENMINDSVSPIPSFSIQLKSEKNKETIVLTVRKGKDTPYLYHNKA
                     YRRADTATVEVDGFELRRLVMQGMNINYESIKAYSQDLTFNTLEEQLKKVLGIEKLSL
                     DILKTLNLYNIDGYYNIAAELLADTNAVKFSGVDIVKFGKDINTFLYRETITEQSLLL
                     QYKRALELYEQYYTYEEIEGFTRVKKELIPKEAFREALANALVHRVWDINSFIQIAMY
                     DDRIEISSPGGLPPGLSENEYLLDRISVLRNPIIADVFNKLDIIEKFGTGITRIKEQY
                     TDSITKPDFCITENRIIIELPVTEKNILRVSEDEKSVYTLLKNEAQLSRNEIDSALGF
                     DKFKTIRVLNNLIDKNIVDKEGKGSAVVYKLK"
     gene            complement(5317..7551)
                     /locus_tag="PKJ65_00650"
     CDS             complement(5317..7551)
                     /locus_tag="PKJ65_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ40377.1"
                     /translation="MRTIYNLNAKWAFTKQANAVPKELPDNWIWVNLPHTWNGIDGQD
                     GGNDYYRNTCYYAKQIDKTDLPTTDCYYLEIQGANSSADVYLNGKHLAHHDGGYSTWR
                     ADITPHLTRDNLLVVAVDNSHNEKVYPQFADFTFYGGLYRDVNLVCVNKSHFSLDYYG
                     GPGVKITPEIKGKNAKVHIETWVTNYKEGQSIRYTIYDAEHKKVSSVETDKTEAMLEI
                     KNVHLWHGRKDPYLYRATIELVEDNKVIDNISSRFGCRTFEIHPENGFILNGEQYPLR
                     GVCRHQDRLDIGNALLRKHHDEDMQLICETGSTTIRLAHYQHDQYFYDLCDQYGVVVW
                     AEIPYISQHNPAANENTISQMKELVIQNYNHPSIVVWGLSNEITMKGDRDPDLIRNHQ
                     VLNDLCHELDSTRLTTVAAVSPCPVDSPYLKIPDVVSYNHYFGWYGGDVSMNGPWFDD
                     FHKKHPDIPIGCSEYGCEALNWHTSRPVQGDYTEEYQAYYHEELIKQLFSRKYLWATH
                     VWNMFDFGADARNEGGTNGRNHKGLVTIDRKYKKDSFYAYKAYLSDEPFVHICGKRYI
                     NRVEDVTKVTVYSNQPEMELLANGVSLGKKKAKCFFYFDVPNKGRTVLTAVAGKQKDE
                     SVINKVEKFDESYRMREKTAILNWFDITAPEGYFSLNDKIEDILKTTRGTLLFMGLFG
                     KMIPKKGEKVMGGFEMNDSMMKMLGGFTVLRLAGMVGTMGLKLTKEDLLDLNAKLNKI
                     KKKE"
     gene            complement(7548..7712)
                     /locus_tag="PKJ65_00655"
     CDS             complement(7548..7712)
                     /locus_tag="PKJ65_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40378.1"
                     /translation="MRYLVAALYTFSALLMYIGLGLIYNLDKKKLAKMNEELEAARVA
                     AETEEKEAVN"
BASE COUNT         1968 a         1759 c         1517 g         2519 t
ORIGIN      
        1 ggtccgtcgc atacaaacat ggcgggaata ccgtactgct caaatttcct tgtttcccag
       61 aaagaggcac cttcgcaaaa ggcaatcttg tcttccaaag tcattttgga aataatgttt
      121 ttaatatcca tatcaaccct ccgttgtata aatcatcgta ccggcaagct gtctaaaatc
      181 gctgccgtcg ggcagagata tcacggcttc acagtttgca ggtacggtta ttgtaaattc
      241 atatttattg tccgtttttt tccacttgct ttgcacttta ccgtaaatac tgttatacga
      301 agcggatgca tgagttaaac tgccgcccgg tcggggagca acaacaaatt gatttttccc
      361 gtttactctt atgccgcaca cggtatcaaa cagccatcgg caaaccgcac ctttggaata
      421 atggttaagt gaaccgagcc cggcaccctg ggtattatac gggccttccc agttctccca
      481 gatggtggtg gcaccgtgaa gaggcatgga gagccatccg ggaatttcct cgttctccaa
      541 taacctgtat gccgcctcta agtcatattc agccagtacg cttaaaatca tgggtgtgga
      601 taagaaaccg gtacccaacc gccaacgata gtattccagt gccttaatca gccgtttcct
      661 tgcaaattca gtttgttctt cattaagcag attaaatgct aaaggacgca ccaattgcgc
      721 ctgtcggtcg gtatccaagt catacttgcc gcccgatacc agttcccggt atgcattttt
      781 acaacctttg ctgtatttct cgaattcccg ggcatccttt tcatagcctg agatttccgc
      841 aatttctgcc attaacccca gaacataaga cgtgtaggcg gtggatactt cgggatgagg
      901 tgttgcaaag tcctgccacc tgaacgagca gacgtctacc ggttccgccc attcgccgta
      961 actttgaccc gtattgacaa gatatttgcg attgccttca gacaacctga ttctcttggc
     1021 aaatacaggc atggttttac cgcaacgact aatcataaag cgggcatagt ttgccatacc
     1081 gttataaaat ctttgaagaa tatccctgtc accgaatatt ttgtaatact gatagggaat
     1141 tataattccc acatccgacc agcctaccga accgttcata ggtttcatat agaagtcggc
     1201 acctccgtcg ggggcaatat ggggcagttt accgttcttt ctttgccagt cgtaaacatc
     1261 ctgtaaatat tttttagaga atgcggcaaa atcaaaaaga taacttgccg tctcaaagaa
     1321 aatctgagca tcacccgtcc atgcgtggcg ttctctggta gggcagtctg tgggcacatc
     1381 caggtgattg ctttttgccg accagacagt ggcatgtacc agaccgttca ccaaaggatg
     1441 agagcagtca aagaaaccgg tccgctccag atttgagtaa acggcgattg cttcaaagtc
     1501 ttggggattt atgtcaaaat ccgcttctac ctgtacatat tgaaacccga atatagaaaa
     1561 gcgtgtcttg tattgattgt gtccttcctt gcaggtatat ataacctgct gcaggggagt
     1621 aacatgcttc ccgaaaacaa gctgaaaatt atcttgagtg aattcgccgt cggacttcat
     1681 catttctccg aagcggagag ttattttctg tcgagctttg gcgtttaaac tgaaagcaac
     1741 ataacccgcc atattctgtc cgaagtccaa cacggtttta ccgccggggg ttataatctt
     1801 tttggcggta aaatgttcct gctcggaaac tcccacatta ttggaagcaa ccggaataac
     1861 cctgtttttt gtcaatttcg ctttcccttt atatgaagcc gtatagtttg catcgtagat
     1921 ttctccgtcc ttattgtctg caaatcgaat gggaccgtca ttgctccaat cccagctgtc
     1981 atcggaaata acggtgtgaa cggtattgtc ggtatgggtt atttccagct gaactaaaag
     2041 cttggttacg ctgccgtatt gattcttcaa gccccaggaa ccgcaggagc cgcgatacca
     2101 gccgtctgca agccatacgg tcagtgtatt ttctccgctt aacaataatt ctgtcacatc
     2161 tacagtctga tattgaattc tttttcggta gtcggtatag ccgggagcca ttacaaatgt
     2221 accgcacctt cggccattaa tcaatgcttc gtaaactccg caggcggtga tatacaaccg
     2281 tgctttggat atatccttgc acttaaaaat tttgcggaaa caatctacgg gataacgctt
     2341 ttttctgcat acaatatagt ttcctgtaat ccactgtgcc ttccaatcgg aaggttgtaa
     2401 cagccctatt tcaaacttag aagaagatat ttcaccttct tttccttcct catcccagag
     2461 tttaaccgac cagtcgataa tatcacgact gcaaagaggc ttgccgtcat aagtaatatg
     2521 agtcatggca tcggacaaaa cttttccgct gtcccagacg gtttctccgt ctcgcttgca
     2581 aataatccgg taggcggtct gttttatccc gccctcacag ttccagaaaa aacggggcct
     2641 gactatatca attcctatcg gttcctgcag atattccgtt cttaatctga tggctttcat
     2701 aactgtcacc ttagtagtta tattataccc tgtcggcata ttctgccaaa agcggttggt
     2761 cgtctgaaaa ttgaaaactc tcatcctgta atatcggaag gaagtattca taaggaatga
     2821 ccggaaattc acatgcatag tcatttgctc cgaaccattt tccttctttg gccgacagat
     2881 ttagtcctct tacaaatttg gtatagttgg agcagtcatg tactacaata tcccatttct
     2941 catcgtctga tgtcttcata agcttcaaag aaagttcacg gctccagaca ggggagatat
     3001 agccgtgtcg ggaaatatac atattctcac cgtagtagtt agcaatatta ttggggttta
     3061 agtgaagctc ggcacagttt atgaacttta tgccggtatt taaaatcata ttcttctttt
     3121 caaaaaaacc ttttataaat tccggtgtca taggagtctc aataccgaca ctccgtatat
     3181 gctctgccgc taatctcatg ttatcaataa ctttatccga acaatttgcg gcacctaaat
     3241 tgaacctgag ttcgtcaaga ccgcatccgg ctaatatctt gaccgattgt ccggtgacca
     3301 atgttccgtt tgtgtacata tgctggtgaa tgtttgcatc gtgaaacttc tttataaccg
     3361 gataatactt ttcaatttcc atgaaaggct ctaaatatac ataagatatg ccggtaggct
     3421 ttttgtgtac ggaaagcaat aaatcgatgt ctttttcata atattttgtt tctcctattt
     3481 cccacatgcc ttcacctacg ggaggtatgt cgtccaattc accgtagtta tagcagaact
     3541 tgcattcaag gttacactta ttggtttttc ttacggcact taagcccgtt cccagaagac
     3601 aggacttaca gccgttcggg aacttatcat catttcccac ataatatgtc ctgttttgca
     3661 gagttttaag gccgcctatg cttgccatca actctcggtt tcttttatcc acaacctctt
     3721 caatctgagc aaatgcggca tacactattt ccgactgatg cgccgtgagc tgttcctctt
     3781 caggtagtgt tgcaaagaat ttaaaccatg tgtaagcatc ttttttggat attttcataa
     3841 tttgtctccc tgcgatattt ttagcggcgc tacttcaatt tatatacaac cgccgatcct
     3901 tttccctctt tatcaacaat attcttgtcg ataaggttat tcaatactct gattgtttta
     3961 aacttatcaa aacccagtgc cgaatcaatt tcatttctgg ataattgtgc ctcatttttc
     4021 aatagtgtat ataccgattt ttcgtcttcc gatactctta aaatgttctt ttccgtaaca
     4081 ggcaattcaa ttattatcct gttttcggtt atgcaaaaat ccggttttgt tatgctgtcg
     4141 gtatattgtt ctttaattct tgttatgcct gttccgaatt tttcaattat gtcgagttta
     4201 ttaaacacat ctgctattat gggatttctt aaaacggata ttctgtccag taaatactca
     4261 ttttccgaca gtccgggggg taagcctccc ggtgaactta tctcgattct gtcatcatac
     4321 atggcaattt ggataaagga attaatatcc cataccctgt gtaccaaggc atttgccaga
     4381 gcctctctga aagcttcttt gggtataagt tcctttttga ctctggtaaa tccttctatt
     4441 tcttcatagg tatagtattg ttcataaagt tcaagtgctc ttttatattg gagcaagaga
     4501 gactgttcag ttatggtttc cctgtataaa aaagtattaa tatcttttcc gaactttaca
     4561 atatctaccc cggaaaactt aacggcgtta gtgtctgcaa gcaattcagc ggcaatgttg
     4621 tagtagccgt cgatgttgta taagttcagt gtctttaata tgtcgagact gagtttttct
     4681 atgcccaata ctttttttaa ttgctcctcc agggtattga aagttaaatc ctgagagtaa
     4741 gcttttatgc tctcataatt gatattcatt ccctgcatta ccaatcttct caattcaaat
     4801 ccgtctactt caacagttgc ggtatccgct ctgcgatatg ctttgttgtg gtataaatat
     4861 ggagtatctt ttccttttct gaccgttaaa actatggtct ctttattttt ttcactttta
     4921 agttgaatgc tgaaactcgg aatgggactt acggaatcat ttatcatgtt ttcaattctc
     4981 aataattgtg cttcagcatc atctaaacct ataatgattc cgttatcatc gataccgaat
     5041 attattcttc cgtcattgta gttggcatat gcacttaccg tttttaaaaa tgttttggac
     5101 acttcctgct tgaactcaac attaatactt tctttcggat ccattgtttt ttctcctacg
     5161 gacttatagt aacatgatta cagtaaaaat gcaacgacaa gcttctcgtt gcatctctcg
     5221 ttgcagaata taacaataaa agcactctcg ttgcacattt gaatgcaacg aggcgattta
     5281 tctgaaatta tgtgcagttg tcttaagtac cgatgattat tcctttttct ttatcttgtt
     5341 aagctttgcg ttgaggtcca gtaaatcctc ttttgtcagc ttcagtccca ttgttcctac
     5401 catgccggct agccgcaaga ccgtgaatcc tcccagcatt ttcatcatgg agtcattcat
     5461 ctcaaaacct cccatgactt tctcaccttt tttagggatc attttaccga aaagacccat
     5521 gaaaagaagt gttcccctgg ttgttttcaa tatgtcttct atcttatcgt taagagagaa
     5581 atatccttcg ggcgcggtta tatcaaacca gttgagtatt gcggtctttt ctctcatgcg
     5641 gtaggactca tcaaacttct caaccttgtt tattacgctt tcatcctttt gtttccccgc
     5701 tacggcggtc aatacggttc tgcctttgtt gggcacatca aagtagaaga aacattttgc
     5761 tttcttcttg cccaaagata cgccgtttgc aagcagttcc atttccggct gatttgaata
     5821 aacggtaacc ttggtcacat cttctacacg gtttatatat cgcttgccgc atatgtgtac
     5881 gaaaggctca tcggataaat aggccttgta tgcatagaaa gaatcttttt tgtacttacg
     5941 gtctattgtt accagacctt tgtggtttcg tccgtttgta ccgccttcat ttctggcatc
     6001 ggcaccgaag tcaaacatat tccatacatg agttgcccat aagtatttgc gtgaaaacag
     6061 ttgctttatc agttcctcgt gataatatgc ctgatactct tcggtatagt ctccctgaac
     6121 gggacgggag gtgtgccagt tgagtgcttc acatccgtat tccgaacagc ctatgggtat
     6181 gtcggggtgt tttttatgga aatcgtcaaa ccacggaccg ttcatggaaa catcaccgcc
     6241 gtaccagccg aaatagtggt tataggatac cacatccggt atttttaaat aaggactgtc
     6301 gacagggcag ggggataccg ccgcaactgt tgtcaagcgt gtgctgtcca gttcatgaca
     6361 aaggtcgttg agtacctgat gatttctgat taggtcggga tctctgtctc ctttcatggt
     6421 tatttcattg gaaagtcccc agacaacaat ggaaggatgg ttatagtttt gtattacaag
     6481 ctctttcatc tgacttatcg tattttcatt tgcagcggga ttgtgttgtg aaatgtacgg
     6541 tatttccgcc cacaccacca ctccgtattg gtcgcataaa tcgtagaaat actgatcatg
     6601 ctgatagtgg gcaagacgaa tggttgttga accggtttcg catataagct gcatatcctc
     6661 atcgtggtgt ttacgaagga gagcattacc tatatccaga cgatcctgat gtcggcaaac
     6721 gccccttaac ggatattgct ctccgttaag aataaagccg ttttccggat gtatctcaaa
     6781 ggttctgcat ccgaaacggg aggaaatatt gtctatgact ttattgtcct ctacaagttc
     6841 aatcgttgct ctgtacagat aagggtcttt tctgccatgc cacagatgca cattctttat
     6901 ttccaacata gcctcggtct tatcggtttc cacactgctt acttttttgt gctcggcatc
     6961 ataaattgtg taacgaatcg actgtccttc tttgtaattt gttacccatg tttcaatatg
     7021 aactttagca ttttttcctt ttatttcggg agttattttt acccccggtc ctccgtaata
     7081 gtccaaagag aaatgggatt tgtttacaca gactaagttt acatcacgat acaatccgcc
     7141 gtagaaggta aaatcggcga actgaggata gaccttttcg ttgtgtgagt tatccaccgc
     7201 tacaaccagc agattgtcac gggtaaggtg aggtgtgata tccgctctcc atgtggagta
     7261 accgccgtca tgatgtgcca gatgttttcc gttgagataa acatcggctg aagagtttgc
     7321 tccctgtatt tccagatagt agcaatctgt cgtcggcaga tcggttttgt ctatctgttt
     7381 ggcatagtag caggtattac gataatagtc gttacctccg tcctgtccgt ctattccgtt
     7441 ccaggtatgc ggaagattta cccatatcca attgtcgggc aattccttcg gaacggcatt
     7501 tgcctgtttt gtaaaagccc attttgcatt taagttatat attgttctca attaactgcc
     7561 tccttttctt cggtttccgc agccacacgt gcagcttcca gttcctcatt catcttggct
     7621 aatttctttt tgtcaagatt gtagataagt cccagcccta tatacatcag cagggcactg
     7681 aacgtgtata aagcggctac gagatagcgc attcttaagg ctacatacaa tgtctgattt
     7741 cattaattgg cttctacata tcc
//
LOCUS       DAOPWC010000014         7636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9609_length_7636_cov_3.686849, whole genome shotgun sequence.
ACCESSION   DAOPWC010000014 DAOPWC010000000
VERSION     DAOPWC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_7636_cov_3.686849"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKJ65_00660"
     CDS             complement(<1..578)
                     /locus_tag="PKJ65_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40379.1"
                     /translation="MKRKAYKLLILIIVAALLLMLCGCAEPVISPSETTVVLPSPTPT
                     PTPTPTVIPETETPSATPEPTPEPTEEIKPDAEDITEKVYVSVDGHYSDKLSDYSFYS
                     KHKLNKGSKIEISSYEKMHSLYIIWDRIPGEWTLNIGDEKLTCGKNGFIHEYIELPCG
                     CTRISIELTNDGATICDVYVFTYGNLPRWVQI"
     gene            710..1753
                     /locus_tag="PKJ65_00665"
     CDS             710..1753
                     /locus_tag="PKJ65_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNZ40380.1"
                     /translation="MTNKLGAAKRAVSVILKTLAVFFTTLALIVVALLGVIYVLVKGP
                     SPTAKKLFVLSVRETSAVGFLADIYLSDEELATLTVSEIVEEPKEKTDTSLIEIPTPT
                     SSLPPSGEDTDITDTVPEEKDIEVVRIKGSLYEAVMLIVKDPLRVFVGTPDELGGKGL
                     KLTEMVEKYDAVAGINAGGFYDPLGGGHEGTPDGMVICNGEVVWGDPDVKLNVSAFDD
                     KGILHVGMMTTQEAMDAKIQWAVSFGPPLIINGKVQSFEKDLISGLNPRTAIGQRADG
                     AVLMLVVDGRRMNSLGATYADLADIMLDFGAVNASNLDGGSSTLMIYEGEVLNVCASV
                     TGPRALPTSFLVR"
     gene            1756..3168
                     /locus_tag="PKJ65_00670"
     CDS             1756..3168
                     /locus_tag="PKJ65_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40381.1"
                     /translation="MRDKAVMHNKDNAGKPKSKFVKGLIIYSICFLVAVFIGLVFFWQ
                     YIKSYEISRPENYMNEFISTVNDDTVRALMTENKKIEVSEFEEENTVINQLYFSLLKN
                     EEYKYRKMPGQYADTSPVYIIYTDSADLGKVVLTSKGDNSAKYGFNLWQTASFTVLDW
                     VLLPEQKTLSITVPEYADVYVNNIKVGESYITDNAVAYTGFEDVNQPFDKRPTGKKYE
                     IPGIYLEAEVTAKDDKGLPLELNKTENEYAYPLVIKETYSYKVTAPKETKVYINGVLL
                     TEKYIENGEAYYPGYENITTYSQVPLLNTYSADNLYYEPDIECYDVNGKSLEVTKASD
                     SKNIFYHYPADEQLKNTHEQHALNFAKTYINFTVNATGDIWGYFPTLNAYLVEGSVLQ
                     SRLKSACASLYWVMGSTLEYKYLKVIDFRPVSETCFTCTMSFSAIHKTYYETRYLEDS
                     FDLVFILYNNKWKVAEMLSE"
     gene            3184..4263
                     /locus_tag="PKJ65_00675"
     CDS             3184..4263
                     /locus_tag="PKJ65_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HNZ40382.1"
                     /translation="MTFKDNGLGITAVIPSLNPSQKLLTTVSGLIEQGFSDIIVVNDG
                     SGPEYDGIFDEVSSTEQCTLLKHEVNKGKGAALKTAFSYIQQRNKDIKGVVTADADGQ
                     HLPEDILKCSQHMTDDKTVILGARNFHQPDVPPKSFLGNKITSFVFKTGCGININDTQ
                     TGLRVFPVKLIPFLLSVEGDRFEYETNMLLEMKPEGIPFKEVEITTVYEDKNVGSHFK
                     AVRDAFRIYRLIFKFMLSSIISFLLDNIVFYVSLLIFANYVENTRILISTVIARALSS
                     FFNFNFNKSSVFKHKCDYKKCLIKYYVLSVSVMLASAGLVTLLSLIIPAEKEIIITLI
                     KIGVDAILFFVSFHVQREWVFKKCK"
     assembly_gap    4366..4465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4680..5477)
                     /locus_tag="PKJ65_00680"
     CDS             complement(4680..5477)
                     /locus_tag="PKJ65_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ40383.1"
                     /translation="MECNLFYTELSRGIYRISDSLHGPNSDGFSDAPGRATQNSYLIQ
                     GQEKAALIDLAVDTPDLFSYACQLTGLPTQVLLTHGHPDHVYNLESVGEAWLHPDDYD
                     LVLKGIPGICFPIHNVVLNPLKDRQKIDLGERILEVIHMPGHTLGSVLFWDKLSGFLF
                     SGDSCSRRLLYGLTPTVPLDEHCKKIAGLAELPINHIYTAHDRCGLPKAHLLRSCELI
                     RQELPKSATTVIIPGIGIMRNLRWGDENTLDYFDMAVEESYVEGLLK"
     gene            complement(5506..6636)
                     /locus_tag="PKJ65_00685"
     CDS             complement(5506..6636)
                     /locus_tag="PKJ65_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNZ40384.1"
                     /translation="MKNHSIRPGKPFLDTEGMRIQAHGGSIFFDAETETYYLYGENKE
                     FSVPGSGIWTWGVRAYASRDFYNWEDKGLIVEPVLDDPLSPFNPRTACLDRPHILFNQ
                     KTEKYVCWMKIMHRDDNRRQTVTIAVADSFLGPYSIVQSGVSPLGMNVGDFDLVDDEN
                     GKAYYYFERVHSELICAELTADYTDVTDVCSHHFPHPYPPFVREAPAHFKRNGLHYLI
                     TSGTTGYFPNRSEVAVSESWHGPFTVLGDPHPGDPTGTSFHSQISSVFKMPGKKNLYV
                     ALADRWMPNAMEIPAAFCEEVFEGFFSGRVPLSEGEHFRESLIRKYGLQGYRSILVQN
                     DTDTSVADYVWLPLRFEEPAPQHPNGMIFIDWLDEWRIEDYS"
     gene            complement(6638..>7636)
                     /locus_tag="PKJ65_00690"
     CDS             complement(6638..>7636)
                     /locus_tag="PKJ65_00690"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ40385.1"
                     /translation="AGWSDVIIELPYSMYRLTGNDLILKKCLPAMKKYADQVIRTAAM
                     RRGGASLDENDRYLWNTGFHFGEWLVPGKEKPGFEACKETAWYIAPFYAFRSVNLLAE
                     ICEKLGDEKSAAYYGSIARKMKAAIEAEVLPLQHEYADYMGRYILALAFELVDGDLEK
                     EYAGKLIELIHSNDYRIGTGFLATPYILKVLDSIGRHDLALKVLMNPNCPGWLYEVSM
                     GATTIWESWSAVAPDGTPQKISFDHYAFGIVDEYFFSVLAGVRTLEPGFASFEVCPDR
                     VIGLDRLKREFICEAGLISVEYTDSVLTVTVPPNTRAVIKWNGETKEVGSGTWRFE"
BASE COUNT         2146 a         1637 c         1631 g         2122 t
ORIGIN      
        1 catatctgta cccatcgggg aagattgccg tatgtgaaaa catagacatc acatattgtt
       61 gcaccatcgt ttgtcaactc aatacttatt cttgtgcaac cgcacggcag ctctatatat
      121 tcatgtataa atccgttctt tccgcacgtc aatttttcat cacctatatt aagcgtccat
      181 tctccaggta ttctgtccca tattatgtat aaagagtgca tcttttcata agacgatatc
      241 tctatttttg atcctttgtt aagtttgtgt ttggaataga aactgtaatc ggaaagcttg
      301 tcggaataat gcccgtcaac acttacatac accttctcgg ttatatcctc cgcatcgggt
      361 tttatctctt ctgtaggctc gggagtcggt tcgggtgttg cagacggtgt ttccgtttcc
      421 ggtataactg taggtgtggg tgtcggtgtg ggtgtgggtg atggtaaaac tactgttgtc
      481 tcgctcggag aaataaccgg ttcggcacaa ccgcacagca taagcaacag agctgcaact
      541 ataatcaata taagcaattt atatgctttc cttttcataa gacaactata ctttatataa
      601 caataccttt cttttctttg ccgcatatcc ccttaacggc accaatatta tattggaaat
      661 tcctctcttt gtgaatataa tatatgaagg gtaagtaagg agacttgtta tgacaaacaa
      721 actcggtgct gcaaaaaggg cggtttcagt aatattgaaa actctggctg ttttctttac
      781 cactttagct ttaatagttg tggctctgct gggagttata tatgtattgg taaaaggccc
      841 ttctcctacc gcaaaaaaac tgtttgtgct gtcggtaaga gaaacaagtg ctgtaggctt
      901 tctggccgac atatatctgt cggatgaaga gcttgcaacc cttactgttt cggagattgt
      961 agaagaaccg aaagaaaaaa ccgatacctc cttaattgaa atacccacac ctacctcttc
     1021 attacctcct tcgggggagg ataccgatat tacagatacc gttccggagg aaaaagatat
     1081 agaggttgtc agaataaagg gaagccttta tgaagcggtt atgcttatag taaaagatcc
     1141 cttaagagta tttgtgggaa ctcccgacga attgggagga aaaggcttaa agcttacgga
     1201 aatggtggaa aagtatgatg ccgttgccgg tatcaatgcg ggcggtttct atgatccctt
     1261 gggaggcggt catgaaggca ctcccgacgg tatggttata tgtaacggtg aagtagtctg
     1321 gggtgacccc gatgtaaaac tgaatgtctc cgcctttgat gataagggta tattgcatgt
     1381 aggcatgatg accacacagg aggcaatgga tgccaagata cagtgggcgg tcagtttcgg
     1441 accccctttg attataaacg gaaaagtgca gagttttgaa aaagacctca taagcggatt
     1501 aaatccccga acggcaatcg gacaacgtgc cgacggagcg gttctgatgc tggtggttga
     1561 cggcagacgc atgaactccc tgggtgccac atatgccgac ttagcggata ttatgctgga
     1621 tttcggagca gtcaacgctt ccaatttaga tggcggctcc tcaacactta tgatttatga
     1681 gggtgaggta cttaatgtct gtgcatcggt aaccggtccg agggctcttc ccacttcatt
     1741 tttagtaagg tgattatgag agataaagca gttatgcata ataaagacaa tgcgggaaaa
     1801 cccaagagta aattcgtaaa aggcttgata atatattcaa tatgctttct tgttgcggtg
     1861 tttatcggtt tggttttctt ctggcaatat ataaagtcct atgaaatatc ccgaccggaa
     1921 aactatatga acgagtttat ctctaccgtc aatgacgata ccgtaagagc cctgatgact
     1981 gaaaacaaga agattgaagt atcggaattt gaagaggaaa acacagtcat taatcaatta
     2041 tacttttcac ttcttaagaa tgaagagtat aaataccgta agatgccggg gcaatatgcc
     2101 gacacatcac cggtctacat aatctatacc gactcggcag atttaggtaa agttgtgctg
     2161 acaagcaagg gtgataattc cgcaaaatac ggcttcaatc tttggcagac cgcaagcttt
     2221 accgttttgg attgggtgct tttacctgag caaaaaacat taagtataac ggtaccggaa
     2281 tatgccgatg tgtatgtcaa taatataaaa gtgggtgaat catatatcac ggacaatgcc
     2341 gtagcatata caggttttga agatgtcaat cagccttttg ataaacgacc tacgggaaag
     2401 aaatatgaga taccgggtat atacctggag gcggaagtaa cggctaaaga cgacaagggg
     2461 ttgcctttag aacttaacaa aacggaaaac gagtatgcct accctctggt tataaaagaa
     2521 acttattcat ataaagtcac cgctcctaaa gagactaagg tttatataaa cggagtatta
     2581 cttacggaaa agtatattga aaacggtgaa gcatattatc ccgggtatga gaatataacg
     2641 acatactcgc aggttccttt gttaaacaca tactcagccg acaatttgta ttatgaaccg
     2701 gatattgaat gttatgatgt aaacggaaag tctttggagg taacgaaggc ctccgatagc
     2761 aagaatatat tctatcacta ccccgcagac gagcagctga aaaacactca cgaacagcat
     2821 gctctgaatt ttgccaaaac atatattaat tttaccgtca acgccacagg cgatatatgg
     2881 ggatatttcc ccacattaaa cgcatatctt gttgaaggca gtgttttaca gtccagactt
     2941 aagtcggcat gtgcaagcct gtactgggta atgggctcca ctttggagta taagtatctt
     3001 aaagttattg atttccgtcc ggtaagtgaa acatgtttca catgcactat gtctttcagt
     3061 gcaatacata aaacatacta tgaaacgcga tatctggaag attcgtttga cctggtgttt
     3121 atactgtata ataataagtg gaaagtagcg gagatgctat cggaataaat aaggagtata
     3181 atcatgacat ttaaagataa cggtcttgga ataaccgcag ttataccgtc attaaatccc
     3241 tcacagaaac ttcttacgac ggtttcggga ttaattgaac aaggcttttc cgatattata
     3301 gtggtaaatg acggaagcgg tccggagtat gacggtatat tcgatgaagt atcttctacg
     3361 gaacagtgca ctctgttaaa gcatgaagtg aataaaggaa aaggagctgc tcttaaaaca
     3421 gccttttcat atattcagca gcggaacaaa gatattaaag gtgtagtcac agcggatgcg
     3481 gacggacagc atcttcctga agacatattg aaatgttcgc agcacatgac cgatgacaaa
     3541 acggttatat tgggagcaag gaatttccat caaccggatg ttcccccgaa aagctttttg
     3601 ggaaacaaga taacttcctt tgttttcaaa acgggatgcg gaataaatat taacgacaca
     3661 caaaccggat tgagagtatt tcccgtaaaa cttattcctt tccttctttc agttgagggt
     3721 gatcgttttg aatatgaaac caatatgctt ttagagatga aacccgaagg tattccgttt
     3781 aaggaagtgg aaataactac cgtatatgag gataaaaatg tagggtcgca tttcaaggcg
     3841 gttcgagatg ctttcaggat atacaggctc atattcaagt tcatgctaag ttcaataata
     3901 tctttccttt tggacaacat tgttttctat gtgtctttgt tgatatttgc aaattatgta
     3961 gagaatacaa ggattcttat aagtacggta atagccagag cattatcctc gttttttaac
     4021 ttcaatttca ataaaagttc ggttttcaaa cataagtgtg attataaaaa gtgtcttatc
     4081 aaatactatg tattatccgt atccgttatg cttgcatcgg caggtcttgt aactttactt
     4141 tccttgatta tccctgccga aaaagaaata ataatcacat tgattaaaat aggggtggat
     4201 gcaatactat tctttgtttc ttttcatgtt caaagagaat gggtattcaa gaagtgtaaa
     4261 tagttattta atcgttttgc tcctcatcta tgtcattaaa ccaaaaatcc atgtattatc
     4321 cgtatccgtt atgcttgcat cggcaggtct tgtaacttta ctttcnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnncttga ttatccctgc cgaaagagaa ataataatca
     4501 tattgattaa aacaggggcg tgtatacaat actttctttg tttctttcca tgttcaaaga
     4561 gaatgggttt tcaagagcta atatgtaaag ggtggtttgt accaccaaat acatatgact
     4621 gcggctcatt tgtggtgaat tggaaagcag ttgtaagaaa tgagtattgt ctttttcttt
     4681 tatttcaaca atccttcaac ataggactct tcaaccgcca tgtcaaagta atccagagta
     4741 ttttcatctc cccatcggag atttcgcata atgcctattc ctggtatgat aactgttgtt
     4801 gccgactttg gaagttcctg tcggataagt tcacaactcc gcagcaagtg tgccttgggc
     4861 agaccgcatc ggtcatgtgc tgtgtaaata tgattaatgg gaagctctgc aagcccggca
     4921 atttttttac aatgttcatc caacgggacc gtaggcgtta ggccgtagag caatcgtctt
     4981 gaacaggaat ccccgctgaa caaaaaacca gacagtttgt cccaaaacag aacgctcccc
     5041 agtgtatgcc cgggcatatg gataacctca aggattcgct cacccagatc aatcttctgc
     5101 ctgtccttca gcggattcag tacgacatta tgtatgggaa aacatattcc cggaatccct
     5161 ttcaggacca gatcataatc atcaggatga agccatgctt caccaaccga ctcaagattg
     5221 taaacatgat ccgggtgccc atgggttaac agtacctgtg tcggaaggcc tgtaagctga
     5281 caagcatagg aaaacaaatc cggtgtatct actgccaaat caattaaagc agccttttcc
     5341 tgtccttgta tcaaatatga attctgtgtt gccctgcccg gtgcgtctga aaatccgtca
     5401 gaattcggtc cgtgaagact gtcggaaatc cgatatatcc ctcttgacag ttccgtgtaa
     5461 aacaaattac attccaatta catcgtctcc ttttataaat aatccttatg agtaatcttc
     5521 aattctccat tcatccagcc agtctatgaa aatcattcca ttcgggtgct gcggagccgg
     5581 ttcctcaaac ctcagcggaa gccagacata gtctgcgacg gaagtgtccg tatcattctg
     5641 cacaagaata ctccggtaac cttgtagccc gtatttcctg atcagagatt cccggaaatg
     5701 ctcgccttcg ctgagcggga ctcggcccga aaagaagccc tcaaaaacct cctcacagaa
     5761 ggctgccggt atttccatcg cattcggcat ccagcggtct gccaacgcca catacagatt
     5821 cttctttcca ggcatcttga acaccgagga aatctgcgag tgaaaggatg tgccggtcgg
     5881 atcgccggga tgtggatctc cgagaacagt aaaagggcca tgccagcttt ccgacactgc
     5941 aacttcgctg cggtttggaa aatacccggt tgttccggat gtaataagat agtgcagccc
     6001 atttcgctta aagtgtgccg gcgcttcccg aacaaaaggc ggatacggat gcgggaaatg
     6061 atgggagcag acatctgtca catctgtgta atctgctgtc aactccgcgc aaatcagctc
     6121 tgaatggact cgttcaaagt aataataagc ttttccgttc tcatcatcga caagatcaaa
     6181 atccccgaca ttcatgccaa gcggagatac cccgctctgt acaatcgaat acggtcctaa
     6241 aaagctgtct gcgaccgcta tcgtaaccgt ctgccgcctg ttgtcatctc tgtgcatgat
     6301 cttcatccag cagacatatt tctccgtctt ttggttaaac aggatatgtg gccgatccaa
     6361 acatgccgtc cggggattga acggtgataa cggatcgtca agcacaggct caactatcaa
     6421 tcccttatcc tcccagttgt agaagtccct tgacgcatag gcacgaactc cccaagtcca
     6481 gataccgcta ccgggaacag agaattcctt gttttctcca tacagataat atgtctcggt
     6541 ttcagcatcg aaaaagatac tccccccgtg ggcctgaatg cgcattcctt ccgtgtcgag
     6601 gaaaggcttt ccgggtcgga tactatgatt tttcatatta ttcgaatctc catgtaccac
     6661 tcccaacctc ctttgtttct ccgttccatt tgatcactgc acgggtattg ggcggaactg
     6721 ttaccgtcag aacggagtct gtatattcga cagaaataag gccggcctcg cagataaact
     6781 cccgtttcag ccggtccaga cctatcacgc ggtccgggca gacctcaaaa ctggcaaaac
     6841 ccggctcaag cgttctgact cccgcaagaa cggaaaagaa atattcatcc acaataccga
     6901 aagcataatg gtcaaagctg atcttttgcg gtgttccgtc aggtgctacg gcactccagc
     6961 tttcccatat cgttgtagct cccatgctga cttcatagag ccagcccggg cagttcgggt
     7021 tcatcaggac cttcaatgcc agatcgtggc ggccgatgct gtccagtact ttcagaatgt
     7081 agggcgttgc aagaaaaccg gttcctatac gataatcgtt tgaatgaatc aattcaatga
     7141 gtttcccggc gtattccttt tccaggtctc cgtcaaccaa ttcaaatgcc agggcaagga
     7201 tgtatcgtcc catatagtct gcatattcgt gttgcagagg aagcacttct gcctcgattg
     7261 ctgctttcat ttttcgggcg atactgccgt aatacgcagc gctcttttcg tcgccaagct
     7321 tttcacagat ttctgccagc aggtttaccg aacggaatgc gtaaaaagga gcgatatacc
     7381 aggccgtttc cttacaggct tcaaatccgg gcttttcctt ccccggaaca agccattctc
     7441 cgaaatggaa accggtattc cagaggtacc gatcgttttc atcaagtgaa gcgccgcctc
     7501 tgcgcatcgc cgctgtgcgg attacctgat cagcatattt cttcatagcg ggcagacatt
     7561 tcttaagaat caggtcattg cctgtcagcc tgtacatact gtacggaagt tctataatca
     7621 catcgctcca cccggc
//
LOCUS       DAOPWC010000015         7587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9688_length_7587_cov_3.815056, whole genome shotgun sequence.
ACCESSION   DAOPWC010000015 DAOPWC010000000
VERSION     DAOPWC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9688_length_7587_cov_3.815056"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..2332)
                     /locus_tag="PKJ65_00695"
     CDS             complement(71..2332)
                     /locus_tag="PKJ65_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ40386.1"
                     /translation="MKTDDKIQKLVDSYGSWFKEDTGFSAAKMTNKHAPYPMLFSPIK
                     INSLTIKNRLVMAPMGNINMAEETGRPNEKMIEYFRERAKGGVGLITTGLIPISFGID
                     HSLIELDKLSYFPRIDRSRTVYVGWRNLAHAVHSYGARIFVQLTPGLGRVGNPQCLTN
                     QFKFPVSASVNPNWYMAEVPCMPLTDAACRKIIKNVGQAAADAKAFGLDGVYLHGHEG
                     YLLEQMTNPAFNRRKLGRYSDWQAFGVEMIQQIRKRAGKSYPIMYRIDLSLALNETYG
                     EKMNKTALKKFKNGRTVEMTLDYMKNLVSAGVDIFDVDLGCYDNWWLPHPPEGMPPAC
                     FADVARIVKKYFEENNILSNAGVKVPVVAVGKLGYPDVAEEVLMQDKADMIMLGRPLL
                     ADPQWPNKARAGLTKDIIPCIGCQEGCINEFVEGGHPQCAVNPRTAFEHLYSEQPASA
                     EKVKNIAVIGSGPAGVMAAVTAAKRGHDVTVYEKQATIGGRLNAGGKPKIKFDIQNYH
                     EYLKNLLETTSKEYKLKVVLNTEATAELLKKEGYDTVIYAAGTKDVELKLPGYEKADK
                     VQAVDVLEKPELVKAAKKVVVIGGGVVGCETAFFLNKELGKEVTVIEMEPYFMNHVCT
                     ANRTYLIYYLEKAGVKLFNCTKLVSYEIGGIKVSRNMSKTVPDPYNTWSPILPPNIEN
                     PLAKPIKEELEGQFIEADIIVQAAGGRCDDTGFYALQKQLPKCEMYNIGDSFKGGKVF
                     DATKAGYAVGLTV"
     gene            complement(2381..4309)
                     /locus_tag="PKJ65_00700"
     CDS             complement(2381..4309)
                     /locus_tag="PKJ65_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNZ40387.1"
                     /translation="MKKDKKNISLWIKKIMLVALDIVAINAATVLSLFVRFELSFSKI
                     DVQYIENARQFLWIDTIFTLIIFTIFGLYSGLWKYASIKELFNIIVACTISYTFKYFV
                     TNWMGISQPRSTIFFGWVLLIILVFATRFSYRFLRYIVHENTQRKGIKRIMVIGAGDA
                     GNMIIREIRSSGILKSKVVCAIDDDKNIHGLNIQGVKIIGGRDQIKEAVKKYKVDEII
                     IAMPSVSSRERKKIYDICKDTQCTLRTIPGMYQIINNEVKLSDLREVQIEDLLGRDTV
                     DINVDEILDYVKGRVILVTGAGGSIGSELCRQIAGHNPKKLVMLDIYENSIYDIQLEI
                     AGKYPGLDMEVLIATIRDEKRIKSIMKKYKPEIVFHAAAHKHVPLMETSPNEAVKNNV
                     FGTLTLVKAADESSVDKFIMISTDKAVKPTNIMGATKRICEMIIQAYNKKSETEFVAV
                     RFGNVLGSNGSVIPIFRKQIEEGGPVTVTHKDITRYFMTIPEAVSLVLQAGYYAKGGE
                     IFILDMGEPVKIYDLATNMIKLSGRTPDVDIKIEITGLRPGEKLYEELLMDEEGIEKT
                     QNNLIYIGRPIQFDEKIFFENLAQLKRMADNEVSCMKLLIAKMIPTYTPEVTDATDEN
                     EVNVCMTQLLAQDNQIKQ"
     gene            complement(4338..5945)
                     /locus_tag="PKJ65_00705"
     CDS             complement(4338..5945)
                     /locus_tag="PKJ65_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ40388.1"
                     /translation="MGKKKTGIKVEKKSTKKNLIKDFFYLEQDTKKLTYSEIVTTAFL
                     YVFLSSFALIVSDKIYENITATKLYALISMFGIWFILMCVGLLEKIRSKKANAEYFKQ
                     AVKNLSVTDIALIAYTLVAFISTILSEYREVAFIGDKARNEGFLMQLLYISMFFMLSR
                     TYVHKLELKIWALASIPVALIGILQLNGYYAGKFTYDILRHGRSFFTTLGNIDVVSTY
                     LVTPIMICTVLYCQSKEKFFRILYFVSAGLCFYLSILIGVDSGFVGMATAIFFMFPFI
                     AKDRLSASRIFHIVSLFLFLIYLEDQISKIGTRRFVFFEKPFNDKLFLGAIAAIIISV
                     IIYFAGKVIKTEKKFIWPVAYFVVATVGAYFVVKKILVMPEPTGSSVKLVLYQFGQIL
                     KGNIQDHYGSNRIYAWRVAYRFFKEKPVIGHGPDTVRALWMREEYIYSTEKYRVVFDK
                     AHNDYLQVLVTTGILGLTSYLVFQFSLLTRSFKKFDQPLVIALVTACICFCAQAFFNI
                     GVPIVSPYGWMLFGMLASILRKKEETA"
     gene            6213..6920
                     /locus_tag="PKJ65_00710"
     CDS             6213..6920
                     /locus_tag="PKJ65_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNZ40389.1"
                     /translation="MEELDIRQIFSYLRRHFWLIVSIVLVAASLSLTVTYFFITPQYE
                     ASATLYVFNNSNRSDTTISSSDITTSQKLIATYSVIMQCDNVINKVIETTGLEYTPGQ
                     IRKMFSGSSVNNTEVMKITIKSEDPVDAMIIANAFIDVAPEEILRVIKAGAVEILDRA
                     VVPSKPSSPNMVKNVAVGVVLGLIVAIAFVILYELFNTKVRTEENLLRVVKAPIIGYI
                     PDLKLYQDEDKKGQIYG"
     gene            6913..>7587
                     /locus_tag="PKJ65_00715"
     CDS             6913..>7587
                     /locus_tag="PKJ65_00715"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNZ40390.1"
                     /translation="MADIEKRELLLFDNKSFPVAEAIKATRTNLMFITAKEKCPVISF
                     TSSHMGEGKSTVCANMALAFAEAGFKTLIIDADMRKPTINKYFKIKSSPGLSDILGGL
                     VKENFIYPTKFKNLHVLPSGTIPPNPSELLTSERMTLLLNELRNTLDYIFIDTPPALI
                     VTDALAISDKVTGTVIAVRQNFSRTNLIKDAVEAINKVNGKFLGFIFNDYNIETKPYN
                     KQYRYGK"
BASE COUNT         2058 a         1721 c         1356 g         2452 t
ORIGIN      
        1 ccctttgaac cgttcagaat atctttttcc tcatcggtga gaaacatttt atattccatt
       61 cttcttctcc ttaaactgtc aatcctaccg cataaccggc tttggttgca tcaaatactt
      121 ttcctccctt gaaagagtca ccgatattgt acatttcaca tttcggaagc tgcttctgta
      181 aagcataaaa tcccgtgtcg tcgcatcttc cgcctgctgc ctgcacaata atatccgctt
      241 ctataaactg tccttccaac tcttctttaa tgggcttagc aagaggattc tctatattgg
      301 gaggcagtat cgggctccat gtgttatacg ggtcgggtac cgtcttggac atatttctgg
      361 aaacctttat ccctcctatt tcataactta ccagtttggt acaattaaac agttttacac
      421 ctgctttttc cagatagtag ataaggtatg ttctgttggc ggtacataca tgattcataa
      481 agtacggctc catctctatt acggttactt ctttgcctaa ttctttattc aggaagaatg
      541 cggtctcaca tcctacaacg cctcccccta taaccaccac tttcttggct gctttcacca
      601 gttccggttt ttccaacaca tccaccgcct gcactttgtc ggctttctca taacccggaa
      661 gtttcaattc cacatctttt gttccggctg catagattac cgtatcatat ccttcttttt
      721 tcagcagctc ggcagtggct tcggtattga gaaccacttt aagtttgtat tcttttgagg
      781 ttgtctccag aagattcttc agatactcgt ggtaattctg tatatcaaac tttattttag
      841 gttttcctcc tgcattaagt cttccgccta tagttgcctg cttttcatac accgttacat
      901 catgtcctct tttagctgcg gttaccgctg ccataactcc cgccgggccg cttcctatta
      961 cagcaatatt ctttaccttt tcagcggatg ccggctgttc gctgtataaa tgctcgaatg
     1021 ccgttctggg attgacggca cattgaggat gtccgccttc cacaaactcg tttatacatc
     1081 cttcctgaca ccctatgcac ggaattatat cctttgtgag tcccgctctt gctttattgg
     1141 gccattgcgg atcggcaagc agcggtcttc ccagcattat catgtcggct ttatcctgca
     1201 tcaacacttc ctcggccaca tcggggtatc ccaatttgcc tacggctact acgggcactt
     1261 taactccggc attggaaagt atattgttct cctcaaaata cttctttact attcttgcta
     1321 catcggcaaa acaagcggga ggcattccct ccggcggatg cggcagccac cagttatcgt
     1381 aacagcccaa atccacatcg aatatatcca ctccggcgga cacgagattt ttcatgtaat
     1441 ccagtgtcat ttccactgtt ctgccgtttt taaacttctt aagtgccgtt ttgttcattt
     1501 tctccccgta cgtttcgttg agagcaagag aaaggtctat tctgtacatg ataggataag
     1561 atttacccgc tctttttctt atctgctgta tcatttccac accgaaagcc tgccagtcgg
     1621 aatatcttcc cagtttcctt ctgttgaatg cgggattggt catctgctcc aagagatacc
     1681 cctcatgacc gtgcagatat actccgtcca gcccgaatgc ttttgcatct gcggcagctt
     1741 gtccgacatt ctttattatc tttctgcacg ctgcatcggt aagcggcata cacggaactt
     1801 ccgccatata ccagttgggg ttaaccgatg ccgataccgg aaatttaaac tgatttgtca
     1861 gacactgagg atttcctacc cttcccagtc ccggtgtcaa ctgaacaaaa atccgtgccc
     1921 cgtatgagtg caccgcatgt gccagatttc tccatcccac atagacagtt ctcgaacggt
     1981 ctatcctcgg aaaatatgac agtttatcca gctcaatcaa tgaatgatct attccgaagc
     2041 ttatggggat aagaccggtg gttatcagtc ccactcctcc ttttgcgcgt tcccggaaat
     2101 attcaatcat tttttcattc gggcgtcccg tttcttccgc catattgata ttgcccatag
     2161 gtgccataac aagtctgttc ttaattgtca gactgtttat ctttatgggc gaaaacaaca
     2221 taggataggg agcatgtttg tttgtcattt ttgcagcact gaatccggta tcttctttga
     2281 accacgaccc gtaactgtca acgagttttt gaattttgtc gtctgttttc atgaatctta
     2341 cctccgtttg ctatttatat attcagaatg taactgttta ttactgctta atctgattat
     2401 cctgagcaag aagttgtgtc atgcatacgt ttacttcatt ttcatctgtg gcatcggtca
     2461 cttcgggggt atatgtcgga atcattttag ctataagcaa cttcatgcag cttacttcat
     2521 tatccgccat tcttttaagc tgtgccaggt tctcgaaaaa tattttttcg tcaaattgta
     2581 tcggtcggcc tatgtatatc aaattgtttt gtgttttttc tattccctct tcgtccatca
     2641 gaagctcttc gtacagcttc tctcccggtc ttaatccggt tatttcaatc tttatatcca
     2701 catcgggggt acggccggaa agttttatca tatttgtggc caaatcatat atttttaccg
     2761 gttcacccat atccaatatg aagatttctc ctccttttgc atagtatccg gcttgcaaca
     2821 ccaaagatac ggcctccgga atggtcataa aatatcgcgt tatatcttta tgtgtgactg
     2881 ttaccggtcc gccttcctca atctgtttcc ggaaaatggg aatgaccgaa ccgttggacc
     2941 ctaaaacatt gccgaaccgg acagcaacaa attccgtttc ggatttcttg ttgtatgcct
     3001 gaataatcat ttcacatatt cttttggtag ctcccattat attggtgggc tttaccgctt
     3061 tatcggtaga tatcataata aatttgtcta cagagctttc atccgccgct ttaacaagag
     3121 taagtgtacc gaacacgtta ttcttcacgg cttcgttggg gcttgtttcc attaaaggta
     3181 catgcttatg agctgctgca tgaaacacta tctccggctt gtactttttc attatgctct
     3241 ttattctctt ctcgtccctt atggttgcaa taagtacttc catatccaat cctggatatt
     3301 taccggctat ctccaactga atatcatata tgctgttttc atatatatcc aacattacaa
     3361 gcttcttggg attatgaccg gctatctgtc tgcaaagctc gcttcctatg gacccgccgg
     3421 ctccggtaac caatattacc cttcccttta cgtaatccag tatttcatcg acattaatat
     3481 ccacagtatc tcttcccagt aaatcttcaa tctgcacttc tcttaaatcg gacagtttta
     3541 cttcgttgtt tatgatctgg tacattccgg gaattgtacg taatgtacat tgggtatctt
     3601 tacaaatatc atatatcttc tttctttccc gtgaagaaac ggaaggcatg gcgattatta
     3661 tttcatccac tttatatttc tttaccgctt ctttaatctg atcccttcct ccgattatct
     3721 ttacgccctg gatattaaga ccgtgaatat tcttatcgtc atctattgcg caaaccactt
     3781 tggatttcag tatgccgctg gatcttatct ctctgataat catattaccg gcatctcccg
     3841 cgcctataac cattattctc tttatgccct ttcgctgtgt gttttcatga acaatgtatc
     3901 gtaagaacct gtaactgaat cttgttgcga aaaccaatat tattagcagt acccatccga
     3961 agaaaatcgt actgcggggt tgacttattc ccatccagtt tgttacaaag tacttgaatg
     4021 tataagatat tgtacatgcc actattatat tgaacaactc tttaatggat gcatatttcc
     4081 acaatccgct gtacaggccg aatattgtaa aaataatgag agtgaatatg gtatcaatcc
     4141 acaggaactg ccttgcgttt tcaatatatt gtacatcgat ttttgaaaaa ctcagctcaa
     4201 accggacaaa cagagacaga acggtagcgg catttattgc tactatatcc agggctacca
     4261 gcattatctt ctttatccat agactgatgt tttttttgtc ttttttcaag gttttactcc
     4321 tccaaaaaca ccttcaatca ggcggtttct tcttttttcc tcagtatcga tgcgagcata
     4381 ccgaacagca tccatccgta cggcgacaca atcggaactc ctatattgaa aaatgcctgt
     4441 gcacaaaagc atatacaagc cgtcactaaa gctattacca acggctgatc aaacttctta
     4501 aaggatcttg tcagcagaga gaactggaaa acaagatagg atgtcagacc cagtattccc
     4561 gttgtcacca gtacctgcag ataatcatta tgcgctttat caaaaaccac tctatatttt
     4621 tccgtactgt atatatattc ttccctcatc cacaaagcgc gtaccgtatc gggcccgtgt
     4681 cctataacgg gtttttcctt aaagaatcgg tatgcaactc tccatgcata tattctgttg
     4741 gaaccgtaat ggtcttgtat atttcccttc aatatctgtc cgaactggta cagtaccagc
     4801 ttaaccgatg agcctgtcgg ttccggcatg acaagtattt tctttacaac gaaatatgca
     4861 cctacggtag caacaacaaa gtacgcgacc ggccatatga atttcttttc tgtttttata
     4921 actttccctg caaaatatat tataacggaa ataataattg ccgctatcgc tcctaagaaa
     4981 agtttgtcat tgaacggttt ttcgaaaaat acgaaacgtc tcgttcctat cttggaaatc
     5041 tgatcctcaa ggtatataag gaaaagaaag agagaaacga tatggaatat cctggatgcc
     5101 gacaacctgt cttttgctat aaacgggaac atgaaaaata ttgcggtagc cattccgaca
     5161 aagccgctgt ccacacctat cagtatggaa agatagaaac agagtcctgc cgaaacaaaa
     5221 tacaatatcc tgaaaaattt ctctttggac tgacagtaca gaaccgtaca tatcattatg
     5281 ggagtgacaa gatatgttga aacgacgtca atgtttccta atgtggtgaa gaaagatctt
     5341 ccgtgtctca atatatcata agtaaatttt cctgcataat atccgtttag ttgaagaatc
     5401 cctattaatg ccaccggtat gctggccaat gcccatatct tcaattccag tttatgcaca
     5461 tatgtccgag acagcataaa gaacatggaa atatacaaaa gctgcataag aaagccttca
     5521 tttcttgcct tatctcctat gaatgcgacc tctctgtatt cggaaagtat tgtagaaata
     5581 aaagcaacca gcgtgtacgc tataagggct atatcggtta cggaaagatt tttgacagcc
     5641 tgtttgaagt attccgcatt tgcctttttt gaacgtattt tctccaacaa accgacgcac
     5701 attaaaataa accatattcc gaacattgat atcaaagcat acagttttgt tgccgttata
     5761 ttctcataaa tcttatcgct gactataagc gcaaaacttg acaaaaacac ataaagaaac
     5821 gctgttgtga caatctcaga atatgttaat ttttttgtat cctgctcaag atagaaaaaa
     5881 tcctttataa gatttttctt tgtgctcttt ttttcaactt ttattccggt ctttttttta
     5941 cccataagcg ctttcccaac ttgttgccat ttgaattata tagtaaagtt taatatttta
     6001 caatgatatc taaaataagg tcattgcgcc tttcattatt attcgcgctt atttcttcca
     6061 atttatgaca actgttaaga aaaacgcaaa actctgttca tagatatctg tattaacggt
     6121 ataaaaggtt aagacttggt ttacttgaca agccaattat gatgccttat tattataaac
     6181 gatgtattaa aaacactgta ggagagattt ttatggaaga acttgatata cgacagatat
     6241 tctcttatct gagacgacat ttctggctga tagtcagtat cgttcttgtt gcggccagtc
     6301 tttctttgac tgttacatac ttttttatta cacctcagta tgaggcgtcg gcaacactgt
     6361 acgtatttaa taactccaac aggtcggata caaccatttc ctcatcagat ataactactt
     6421 cgcagaaact tatagccact tattcggtta taatgcagtg cgacaacgtc ataaataagg
     6481 taatagaaac aacgggtctg gagtatactc ccggacaaat cagaaaaatg ttttcagggt
     6541 cttcggtgaa taacaccgaa gttatgaaga taaccatcaa gagcgaagat ccggtagatg
     6601 ctatgattat agccaatgcc ttcattgatg tagcaccgga ggaaatactc agagtcatta
     6661 aagcgggggc cgtggaaata ctggacagag ccgtagtacc gtcaaagcct tcctctccca
     6721 atatggttaa aaatgttgct gtcggtgttg ttttagggct tattgtcgct attgcttttg
     6781 taatattgta tgagttattt aacaccaaag tgcggactga agaaaacctg ctgagagttg
     6841 ttaaagcacc tattataggg tacattccgg atctgaaact atatcaggat gaagataaga
     6901 aaggacaaat ttatggctga cattgaaaaa cgtgagttac ttctttttga taacaaatcc
     6961 tttcccgttg cggaagcgat taaagctaca agaaccaact taatgtttat aaccgcgaaa
     7021 gaaaaatgtc cggttatctc ctttacaagt tcacatatgg gtgaaggaaa atctacggtc
     7081 tgtgctaata tggctctggc tttcgccgaa gcgggattta agacattaat aatagatgcg
     7141 gatatgcgta agcccactat taataaatac tttaagataa aatcctcacc gggactcagc
     7201 gatatactcg gcggtcttgt aaaggaaaac tttatatatc ctacaaaatt caagaatctt
     7261 catgttttac caagcggcac tattccccct aatccgtcgg aactgctgac gtccgaaaga
     7321 atgactttac tgctgaacga attgcgtaat actttggact atatatttat tgacacgcct
     7381 cctgcactta ttgttacaga cgccctcgcc atttcggata aggttacagg tacggttata
     7441 gcagtcaggc agaatttttc caggactaat ttaatcaagg atgccgtaga agcgataaac
     7501 aaagtaaacg gaaagttttt gggctttatt ttcaacgatt ataatatcga gacaaaaccc
     7561 tataataagc aatacaggta tggtaag
//
LOCUS       DAOPWC010000016         7515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9814_length_7515_cov_3.097721, whole genome shotgun sequence.
ACCESSION   DAOPWC010000016 DAOPWC010000000
VERSION     DAOPWC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9814_length_7515_cov_3.097721"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKJ65_00720"
     CDS             <1..776
                     /locus_tag="PKJ65_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40391.1"
                     /translation="MCDDHNNNRTSKALWIDGTGMTEKLEFFPNGQTVGPVTYLESGP
                     LLDQMSARKIGLFKLELDEFTGTMITGRHFVRLQENPGARLAFIQDGATVTFAGKDIT
                     TAYNLTLFDFGYYKGAFNREEILASAGPDGEHFTFAFAYHGELPGMAEFSIATGMTEG
                     TKVNIYKYDPETGIFTLIAGDVTVGRDGIVTYRNNTMSEYLITTKTLEDAKISDVADM
                     QSPEGVNIWLFISIGLAVLLCAAAVLLIVFYRKRPKKAK"
     gene            complement(865..953)
                     /locus_tag="PKJ65_00725"
     tRNA            complement(865..953)
                     /locus_tag="PKJ65_00725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Ser,seq:cga)
     gene            1261..1458
                     /locus_tag="PKJ65_00730"
     CDS             1261..1458
                     /locus_tag="PKJ65_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40392.1"
                     /translation="MSTTNINIRVDSSLKKEAEDLFRDLGLNPETEAALAEYEEMKKN
                     PGNYKRYNSFKEAMDEVLSDA"
     gene            1451..1723
                     /locus_tag="PKJ65_00735"
     CDS             1451..1723
                     /locus_tag="PKJ65_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNZ40393.1"
                     /translation="MLKAVLSNQFKKDLKQAKKRGFKLEKLNKVVNMLARQEPLPVKH
                     RDHNLTGKYRNFRECNVEPDRLLIYKIEKTELELFLFRTGTHSDLF"
     gene            1952..2028
                     /locus_tag="PKJ65_00740"
     tRNA            1952..2028
                     /locus_tag="PKJ65_00740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1986..1988,aa:Arg,seq:cct)
     gene            2051..2311
                     /locus_tag="PKJ65_00745"
     CDS             2051..2311
                     /locus_tag="PKJ65_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ40394.1"
                     /translation="MVRKTAYTTVIRRLDPLGRIVLPKKYRKKCEMNVKDDVEMYLEG
                     SAICVKKYNTRCKLCGETDNLIVFKGANLCPACVTGIKRLNF"
     gene            2311..2766
                     /gene="tadA"
                     /locus_tag="PKJ65_00750"
     CDS             2311..2766
                     /gene="tadA"
                     /locus_tag="PKJ65_00750"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNZ40395.1"
                     /translation="MLKKMMKEALEQASKALEHDDVPVGAVIVKDGNIVAKAHNRRFC
                     DKDSTAHAEILAISQACAFFNTQFLDGCDIYVTCEPCIMCAGAIIQSRIKNLYFGAYD
                     KKAGCAGSVLNVFDTAFNHKVSVTGGIMEADCSELLNIFFKNKRIKTNE"
     gene            2887..4485
                     /locus_tag="PKJ65_00755"
     CDS             2887..4485
                     /locus_tag="PKJ65_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNZ40396.1"
                     /translation="MLGNKIHELRKKHSLTQEAFASLINVSRQAVQKWERGISLPDCD
                     KLISISRVFNVSVDYILDLETRDPHQEFRVKKEIQPSYQALHWWEVYSTQLSIEYKQC
                     LDEGKDITKLKTLMESIQALETSTYKEQLADTLHNMILNTPYAPDYPYNEPDELNLIY
                     PLTKHDFHYPFGKLSTNVLKSKIKGAWMGRICGCLLGKTVEGIRTEELIPLLKETGNY
                     PMHRYILKSDMSEEIYSKYKYNLRNRCYADAIEYAPSDDDTNYTVLSSVIVDKFTDRF
                     TSYDVAKTWLEKQPKSAYCTAERVAFLNIAKGYLPPETAIYKNPFREWIGAQIRVDYY
                     GYINPGDPKKAAHMAWKDARVSHIKNGIYSSMLIAAIIAAAACCNNVKDAINYGLYQI
                     PQTSRLYKAVMKVLKQYESGMTCEKCFESIHEEYDEHSGHHWCHAISNTCIVIASLLY
                     GNNDYAKSICLAVQTGFDTDCNAATVGSVIGMLKGIEYIPDYWTAPIKNKLETSIFGI
                     GIVDTDYLADITYKHVMSYSDIYK"
     gene            4847..5323
                     /locus_tag="PKJ65_00760"
     CDS             4847..5323
                     /locus_tag="PKJ65_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40397.1"
                     /translation="MPPLQQAPYGRPYVQPKKSKAGVVIAVIAIIVVFGLLVAALSNG
                     WFSSAPVIKKACVATALDPETYEPLNRVRNIPGTEPTIYVAAYIQNVNQEVSVVSVWY
                     HLDSDESFISDYIYIDYDAWFYFSLDQSEFEFPVGDYVVELYVDDYLEETVEFTIY"
     gene            5649..7181
                     /locus_tag="PKJ65_00765"
     CDS             5649..7181
                     /locus_tag="PKJ65_00765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ40398.1"
                     /translation="MRKDFNKKDRLLINNITEKNSKQISDSVNAAELETERLAALNEQ
                     LEEKKEQLQIILNSTAEAIYGVDMEGICTFCNKSCLRILGYETEDQLLGKKMHELLFD
                     SNDERACKVIKCMEEGRTLHIDNIKFLKADKSTIHVELHSYLQYKNDKVIGAVVTFLD
                     ITQKLSDMECIRYLSFHDVLTDLPNRRFFEQRIKEIEVSDNLPYSVIFADINGLKITN
                     DVFGHDIGDRLILKSAQALKNSANNNSFIARVGGDEFIVLLPKTDRGEAYDIMNAVKE
                     EIHNARIASITFSISIGAETITDDKTSIEQAINMAEDRMYREKVMNRRSTNTDMLNEL
                     VITLHERSPREVVHSKNVSALSEKIALAMKLPEPMVKKAKIAGYMHDIGKVVLDEKIL
                     KKEVYSDEEREQFHLHPVYGYRILNLFDDMLDIAEGIYSHHEKWNGSGYPKGLKESDI
                     PLIARIVAVAEAYDALTNTKNICALSKEEALDRIGKSSGVTLDPYIADLFVDIMQNSV
                     TA"
     gene            7322..>7515
                     /locus_tag="PKJ65_00770"
     CDS             7322..>7515
                     /locus_tag="PKJ65_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40399.1"
                     /translation="MGERNKVLKRYLSNNNRFKSDNPDYGRAFLLNAALAYMSIICMI
                     FGILNIIKTDHPVFIYFYFAV"
BASE COUNT         2479 a         1367 c         1578 g         2091 t
ORIGIN      
        1 atatgtgcga tgatcataat aacaacagaa cttcaaaagc tttatggata gacggtaccg
       61 gaatgacgga gaagctggaa tttttcccga acggtcaaac agtcggtccg gttacctatc
      121 ttgaaagcgg tcccctgctg gatcaaatgt ccgccagaaa gataggcttg ttcaagttgg
      181 aactggacga atttaccggc accatgatta ccggaagaca ttttgtcagg ctgcaggaaa
      241 atccgggagc aagactggcc ttcattcagg atggtgctac cgtcacattt gcaggaaagg
      301 atataacaac cgcctacaac cttaccctgt ttgatttcgg ctattacaaa ggtgccttca
      361 acagagagga aattttagct tcggccggtc ctgacggtga acacttcact tttgcatttg
      421 cctatcacgg tgaactgccg ggcatggcgg aattcagcat cgctaccggt atgactgaag
      481 gcacaaaggt aaacatttat aaatatgatc ccgaaacagg tatatttaca ttgatagcag
      541 gcgatgtcac tgtaggtcgg gacggtattg taacctatag aaacaataca atgtcggaat
      601 atctgataac caccaaaact ctcgaagatg caaagatatc ggatgttgcg gatatgcaaa
      661 gccctgaagg agtaaatatc tggttgttta taagtatcgg tcttgctgta ttgctttgcg
      721 ctgctgctgt cttattgatt gtattctacc gaaaacgacc gaaaaaggcc aaatgacggt
      781 tttttacaca acttttggtc aacagtcata gaatatcata ggaaaacgca cctttgtcct
      841 ggcagacaaa agtgcgtttt ttgttggcgg aggaggtggg attcgaaccc acgtgtcctc
      901 tcggacaact cgatttcgag tcgagctcgt tatgaccact tcgatactcc tcccctcgcc
      961 ttatatatat tacttaaata aatcatttaa atcaatagtt taagtaagga gtgtttcggt
     1021 tttttagttg tagaaatttt gaatgataat tatatctgtg tggatacatt gttatctctc
     1081 gcagcaagtt accggcaagt taaattacgt ctaatttaaa atattcagtt tagataagca
     1141 tactgaatac ttaactcagg agaaacatca acgcaaacaa tgctttacac tgtgctgaaa
     1201 tctcttgcac ctgagattat aataaatcta taataaaaag aaggatagga ggtgacggct
     1261 gtgtccacta caaatatcaa tatcagagta gattcttctc taaaaaagga ggccgaagat
     1321 ttgttcaggg acttaggtct gaaccctgaa actgaagcag ccttggcgga gtacgaagag
     1381 atgaagaaga atcccggcaa ctataaacgc tacaattcct ttaaggaagc tatggacgag
     1441 gtactctccg atgcttaaag cggttctgtc aaatcaattc aaaaaagatt tgaagcaagc
     1501 aaaaaaacgc ggttttaaac tcgaaaaact taacaaagtt gttaatatgc ttgcccgcca
     1561 ggaaccacta ccggtaaagc accgtgacca caacctaacc ggaaaatatc gcaatttccg
     1621 tgaatgtaat gtagaacctg atcggctttt aatttacaaa attgaaaaaa cggaacttga
     1681 actgtttctg tttcgtaccg gcacacattc ggacctgttt taaagtcaca tttctatagc
     1741 aaggacggat aaacaatggc aaattggaaa gactataaaa attatgtaaa agaaatcaaa
     1801 caagaaatcg gaaaagatat agaggaagtt gaggccgttt cccgcattcc tcggttgcaa
     1861 gaatcgaatt cgattccgaa cttgttaatc acttccgtgt ctatattttc tttgacattg
     1921 aatcgctgtt taggtacaat atggagcatg ggcactcata gctcagcagg atagagcgtc
     1981 ggtttcctaa accgcaggcc ggaggttcga atcctcttga gtgcaccagt ttttatagga
     2041 ggcaacataa atggttagaa aaactgcata caccactgtt atcaggagac tggacccgtt
     2101 gggaaggata gtactgccta aaaaatacag aaagaaatgt gaaatgaatg tcaaagatga
     2161 cgtcgaaatg tatcttgaag gttccgctat atgcgttaag aaatataata cccgttgcaa
     2221 attatgtgga gaaaccgata acctgatagt attcaaagga gcaaacctct gccctgcttg
     2281 tgttacggga ataaagagac ttaatttctg atgttaaaaa aaatgatgaa agaggctttg
     2341 gagcaagcgt ccaaggcttt ggaacatgac gatgtaccgg tcggtgctgt tatagtaaaa
     2401 gacggaaata tagttgcaaa agctcacaac agaaggtttt gcgataaaga ttccactgca
     2461 catgcggaga ttttggcgat aagtcaggcg tgtgcttttt ttaatacgca gtttcttgac
     2521 ggttgtgaca tctatgtcac ctgcgaaccc tgcataatgt gtgcgggagc aataatacag
     2581 tcaagaatca agaacctgta tttcggcgcc tatgataaga aggccggatg tgcaggctcg
     2641 gtactcaatg tctttgatac cgcttttaac cataaggtgt cggtaaccgg cggaattatg
     2701 gaagccgatt gttcggaatt attgaatatc ttttttaaaa ataagagaat aaagacaaat
     2761 gaataagtca acacagcaca tcatttgatt tcatgcttta tgctacttac agtttcataa
     2821 atgtatacta aagtatattg aactctctct ttgtatatcg tattattgac atatggaggt
     2881 attgatatgc ttggtaataa aattcatgaa ttgcgaaaaa aacacagtct tacacaggag
     2941 gcatttgctt cactgattaa tgtaagcaga caggctgtac agaaatggga aagaggcata
     3001 tctttaccgg actgtgataa actcatatcc ataagcaggg tttttaatgt atcggtggac
     3061 tacattctgg atctggaaac gcgagatccg catcaggagt tccgtgtaaa aaaagagatt
     3121 caacccagtt accaggcttt gcactggtgg gaagtatatt ctacacaact ttcaatagaa
     3181 tacaagcaat gtcttgacga aggcaaagat attaccaagt tgaaaactct tatggaaagc
     3241 atccaggctt tggagacatc cacttataaa gagcaactgg cggacacgtt gcataatatg
     3301 attctgaata ctccctatgc accggattat ccgtataatg agcccgatga gcttaatttg
     3361 atttatcctt taaccaaaca tgatttccac tatcctttcg gtaaactttc aacgaatgtt
     3421 ttgaaaagta aaattaaagg agcgtggatg ggccgcattt gcgggtgtct gctgggcaaa
     3481 actgtggaag gtatccgcac ggaggagctt attccgctgc ttaaagaaac gggtaattac
     3541 cccatgcaca gatacatact caagtcggat atgagtgaag aaatttattc gaaatacaag
     3601 tataacttaa gaaacagatg ttatgccgat gcaattgaat atgctccttc ggatgacgat
     3661 accaactata cggtactctc ctccgttatc gtagataaat tcaccgaccg gtttacttca
     3721 tatgatgtag caaaaacctg gctggaaaaa cagccgaaga gtgcttattg cacagccgaa
     3781 agagtagctt ttctgaacat agcaaaggga tatctgcctc cggaaacggc aatttacaag
     3841 aatcctttca gagaatggat aggtgctcag ataagggtgg actactacgg atatataaat
     3901 cccggagatc ctaaaaaagc ggcacacatg gcttggaaag atgcaagagt ttctcatatc
     3961 aagaacggta tatactcctc aatgctgata gctgcaataa ttgccgcagc ggcttgttgc
     4021 aataatgtga aagatgccat aaattacggg ctttaccaga tacctcagac atccagattg
     4081 tataaagctg tcatgaaggt attgaagcaa tatgaatcgg gaatgacttg cgagaagtgt
     4141 tttgaatcta tacatgaaga atatgatgaa cattcgggac atcactggtg tcatgccatt
     4201 tccaatacct gcatagtaat agcttcgctc ctgtacggta acaatgatta tgcaaaatca
     4261 atatgcttgg cggtacagac agggtttgat accgactgta atgccgctac ggtaggttcc
     4321 gttataggta tgctcaaagg aatagagtat atacccgatt attggaccgc tccgataaaa
     4381 aataaactgg agacctccat tttcggtatc ggtattgttg ataccgacta cctggcagat
     4441 ataacgtata agcatgtgat gagctactcc gatatttata aatagcggta aaaatataaa
     4501 cccgatataa aaaagtattt cttgttgccg atttaatatt gttcatgata aaattatgtt
     4561 gtatagcaga tatataatgt aatacagtta ttatttcaaa aaattaaaag agaaatacaa
     4621 agggggtagt aaaatgttct gtccgaaatg tggtacaaat aatggtgata acacgagatt
     4681 ctgttttaat tgcggctatg ctttgtccgc acctcaacag ccgaaccaac agttgccgcc
     4741 actgcaacaa cctaaccaat atcaacaaca gttaccgcct ctcaggcaac ccaaccaata
     4801 ccaacaacag ttaccgcctc tcagacaaac taatcaattc caacagatgc cgcccttaca
     4861 gcaggcaccg tacggacgac catatgtaca acctaagaaa agtaaagcag gtgtcgtaat
     4921 tgccgtaatt gctattattg tggttttcgg tttacttgta gcggcattga gcaacggttg
     4981 gttttcttct gctcccgtta ttaaaaaggc atgtgtagcc acagcgctgg acccggaaac
     5041 ttatgaacct ctcaaccggg tcagaaacat acccggtacc gaacctacca tttatgtcgc
     5101 ggcttatata caaaatgtca atcaagaggt aagtgttgtt tccgtttggt atcaccttga
     5161 ttccgatgaa tcctttattt ccgattatat atatattgac tatgacgcgt ggttctactt
     5221 cagcttagac caaagcgagt ttgaattccc tgtcggagat tatgtagtgg aattgtatgt
     5281 tgatgattat ctggaggaaa cagtggagtt tactatttat taacatcaac aacagtccat
     5341 cctataacat atattaatgc aagcatcctt accgtttgat agaggcatct gtaaaaagcg
     5401 gcagaaatgc cgtgaagaat ttgcttattc agcaaataaa cattttttta catttttctt
     5461 tatttaaatc ttttttgtga tataatcatt agtggtcaac tatgacaaaa attataaaaa
     5521 acatggctgt ataaagcggt gttacatata tatcggttac cggactaact atttgaaaat
     5581 taatttaaag acttgacatt acctcttctc aaatagattt gccgatgtaa ctagatgagt
     5641 tatgggttat gagaaaagat tttaacaaaa aagacagatt attaattaat aacattactg
     5701 aaaaaaatag caaacagata tccgattccg ttaatgctgc agagctggaa acagaaagac
     5761 ttgcagcttt aaatgagcag ttggaagaga aaaaagaaca gttgcagata atactgaact
     5821 ctactgcgga agctatttac ggagtagata tggaaggaat atgcactttt tgtaataaaa
     5881 gttgtctgag aatattaggt tatgaaaccg aggaccagct tctgggtaaa aaaatgcatg
     5941 aactgttgtt tgacagcaat gacgaaagag catgtaaagt tatcaaatgc atggaagaag
     6001 gacggacttt acatattgat aatataaagt ttttaaaagc ggataaaagc actattcatg
     6061 ttgaactgca ttcttatctt cagtataaaa atgacaaggt tataggtgcg gtggtaactt
     6121 ttctggatat aactcaaaaa ctgtccgata tggagtgtat aagatacttg agttttcatg
     6181 atgtgcttac cgatcttccc aacaggcggt tttttgagca aagaataaaa gagattgaag
     6241 tatcggataa cctgccttat tcggtaatat ttgccgatat aaacggtctt aagataacca
     6301 atgatgtatt cggacatgat ataggtgaca gattaattct gaaatcggca caggcgctga
     6361 aaaacagtgc taacaataat tctttcatag cccgtgtcgg aggagatgaa tttattgtat
     6421 tattacctaa gaccgatcgg ggagaagcat acgatataat gaatgcggtt aaagaggaaa
     6481 tacataatgc cagaatagca tctataacat tcagtatatc cattggagcg gagacaatca
     6541 ccgatgataa aacatccatt gaacaggcaa tcaatatggc cgaagacagg atgtatcgtg
     6601 agaaagtcat gaacagaaga agtaccaata ccgatatgct gaacgagttg gtcataaccc
     6661 tgcacgaaag aagtcccaga gaagtggtcc attccaagaa tgtatcggca ttatcggaga
     6721 aaatagcatt ggccatgaaa cttcccgaac ctatggtcaa aaaagcaaag attgccggat
     6781 atatgcatga tatcggaaaa gtggttcttg atgaaaaaat actcaaaaag gaagtttatt
     6841 ccgatgagga aagagagcag ttccatctac accctgttta cggatacaga atattaaatc
     6901 tttttgatga tatgctggac atagccgaag gcatatactc tcatcatgag aagtggaacg
     6961 gttcagggta cccgaaaggc ttaaaggaat cggatattcc tctaattgca agaattgttg
     7021 cggttgccga agcatatgat gcactgacaa acactaaaaa catttgcgct ttatcaaaag
     7081 aagaggcctt ggacagaatc ggaaaatctt caggtgtaac gcttgatccc tacatagcgg
     7141 atttattcgt ggacattatg caaaatagtg taacagcatg agtttcatat taagaaaaga
     7201 ataatagaga aagaatattt acctagaatt taccttgaat taccaataat gcatgttttt
     7261 gcttgcactt tatttacaga tagtgtatat tatgctggaa tgattattta gagggtgtac
     7321 tttgggggaa agaaataagg ttttaaaaag atacctttca aataacaaca gatttaagtc
     7381 cgataatcct gattacggaa gagcattcct tttaaatgcc gctcttgcgt atatgtctat
     7441 catatgcatg attttcggca tcctgaatat aataaaaacc gatcatcccg tctttatcta
     7501 tttctatttt gcggt
//
LOCUS       DAOPWC010000017         7464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9894_length_7464_cov_3.528816, whole genome shotgun sequence.
ACCESSION   DAOPWC010000017 DAOPWC010000000
VERSION     DAOPWC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9894_length_7464_cov_3.528816"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKJ65_00775"
     CDS             complement(<1..219)
                     /locus_tag="PKJ65_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40400.1"
                     /translation="MNQRERFLNVMNFRPVDRLPMIEWAGWWDKTILRFISEGVPDMP
                     DKEDIKHEYFGLDRLKQYWIGARGRGYNQ"
     gene            complement(259..>1747)
                     /locus_tag="PKJ65_00780"
     CDS             complement(259..>1747)
                     /locus_tag="PKJ65_00780"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNZ40401.1"
                     /translation="VAALINRAIDTISEEDIKTIIASEAVQYEKMFNRTELRVTIALS
                     AVIFAILTVAMILIIYLKYKENKNLFLNTDSLTGYPNIKGMKAVTDVSISMLNISTYC
                     MVNLVVYNLNYIREVYGYTEADKVQTDIAVLLDNHIYNNEILARANNDDFILLLQFTE
                     REEMIGRLSNIVEKVNEKYDLKNSDYPVVLSIGIYNPKYEELNMDVLMQKSLQARQFV
                     KEKDDVRYKFYDDTVRIMTVEEKLMQQEFLASMEKDEFLIYIQPKILLADNSIYGGEI
                     LSRWYNPRRGLIRPGEYIPVFEKSGIITTFDMYMFEKACQLLQQSLKEGRQVVPVSVN
                     FARSHFANIDFCDRIFAIATQYEVSPNYLNIEVTESLIAENAKMMKDIVNKLKEMGFT
                     ISLDDFGTGYSSFSDLPNFMLDYLKLDRSMIYNLEDSKTKKMIKGITDLAHNMDSLII
                     CEGVETKEQADILRSLDCDIAQGYYYYQPMPYEEFEKLLQSKQID"
     assembly_gap    1748..1757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1758..2177)
                     /locus_tag="PKJ65_00785"
     CDS             complement(<1758..2177)
                     /locus_tag="PKJ65_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40402.1"
                     /translation="MKKRFLVLSIICILVMSAIYGYAAEEQTFVLTKEEQAVVASYPV
                     VYVALDRNSFPFEFYNYAEKQYEGVVVRILDHVHQLTGFQFEYLKTKDFEQALQYGFN
                     FQADITVSKVNKYAGFMQSRQLVKFKGYDVGMQFTQRM"
     gene            complement(2178..2621)
                     /locus_tag="PKJ65_00790"
     CDS             complement(2178..2621)
                     /locus_tag="PKJ65_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNZ40403.1"
                     /translation="MIAFKGIKKVLFGYSKKDVIMYIELLNEDLNSRIAEKDRQVNLI
                     LEQNKMLLKRLEMLEKNKDDIALSLIHAKKKADNILADARKQADIILKDTMEEKERQA
                     EQYKQVEEEFEEFRQSVSKVLKSFIRGAEGIKKDCDKAHKLNIGE"
     gene            complement(2671..4074)
                     /locus_tag="PKJ65_00795"
     CDS             complement(2671..4074)
                     /locus_tag="PKJ65_00795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ40404.1"
                     /translation="MFIETARAILISIGIFYSLYLFVYATFLLVSVTIGASELYRTKQ
                     LSTLHNVISHDYYFPVSIVVPGYNEEVTICDTVHSLLNLDYRLYEIIVVDDGSKDNTS
                     KLLVEEFKMTKVRRKIKKSIRCQDEEFIYESYAYKVPIVLIRKKNGGKADALNMGINA
                     SRYPYFVCMDADSMLQSDALERIVRPVLTDENLIACGGVIRIANGVVLENGKVKKYEM
                     PDNMVAAMQVLEYERSFLASRILFDMFNGSLIISGAFGLFKKDMVIAVGGYDHTTMGE
                     DMELVVRLHVFCLKNQIPYTIKYATDAVCWSQAPDNMRDLRKQRRRWHLGLYQSLTKH
                     KQILFSSKFGLVRTVSYMYFLIFELLSPYIEIFGILSVIAMFLLGMVTTRVAIAYTAI
                     SLLYGAILTLSAFFSRIHTQNIELRAKDVWKAIGACIFENVGFRVILAYTRMTAFIGY
                     KKRKLQWGKIKRVKFNQ"
     gene            complement(4064..5194)
                     /locus_tag="PKJ65_00800"
     CDS             complement(4064..5194)
                     /locus_tag="PKJ65_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ40405.1"
                     /translation="MANEVRIDLILIIYTAICVMMICFNTVFLLYLRYRDFIFKDKRE
                     KIRQAIKDQIELVSKNEEIDKQKQQYLYRNFTKIRFLKAFHEIVKEQAAVDYESTFAY
                     LKQCKPLFEYLIIEFDNKENSKKGYLAFLIGEFRLCDQFVYDFIVSAMISYTTNKSVF
                     VRQNALHTLYTSDNGEAILTAMLRMQEMKIKHNNKLIADGLMLFHGDRAHLASYLWKE
                     FGSFDVSTRVAIVNFIKMSTLDYSEKFLKLLKDKHSHKEIRLAVIRYFGRIAYAPAGQ
                     YLVELLRQKNSEWEYRAIAATALSAYKCDEVVEALKEALSSDNWYVRFNASESLVGMD
                     VKYMDLVDIYNGSDRYAREILEYKVETERLKRKTRREKRDVY"
     gene            complement(5184..7337)
                     /locus_tag="PKJ65_00805"
     CDS             complement(5184..7337)
                     /locus_tag="PKJ65_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40406.1"
                     /translation="MKKFLIATGIILILALGINYVLFSTNFFIDLHPNTPVTTSFITD
                     EENIYILKNGQYEKFQIKGVNIRSAIPGKFGTDYAIEYDDYLRWLKQIKEMGANTIRV
                     YQIMNVDFYNALYDFNKDNEDPLYLLHGLTVSDYVQNSHIDAKDEDFYKEIIKQTYIL
                     VDIIHGKRQLVLNQQSGYGFYMKDISPWVLGYVLGSNWNSNTIAYTDRLHKVHEGYSG
                     KYMYTTEDATAFESVLAMIGDTIITYESARYKEQRLVSFFNWPSTDPFVYPDYITEFF
                     KKCAEVDVEHIRTTDAFISGHFASYHAYPYYPGYLNYFENKDFWFKDAEGNVNTYTAY
                     LKMLKLHHSIPIVITEFGVPTSRGMAHEDTNRGYNQGRNTEYEQGQIIVNTYEQIKSA
                     DCAGSLVYVWQDDWSLTTWNTQHSVDERRCVYWSDAQSSDQGFGILTFDPGEKKVLCH
                     VDGDINEWVGIEPVAVNGDMSVSMQYDEKFIYYLVYKKDYDRERDILYIPIDITRKSG
                     SNYCKQHDVRFDCYADFLIVLNGINDSRVLVQERYDVLRAMYNQYIAGENPHSRPPHI
                     NSPVFNKINLILQLEALEKVNHPGIKAQVYETGILTHGNSNPAHKEYNSLADFHIAGN
                     YIEMRIPWNLLNFSDPSNMFIHDDYYEHYGVESIGIDKMYVGVGSAENRGHIIEMFPF
                     SLKGWNKVTYHERLKASYYMIQKMWTDEERTADGE"
BASE COUNT         1990 a         1703 c         1289 g         2472 t
ORIGIN      
        1 ctgattatat cctctccccc tggctcctat ccaatactgt ttcaatctgt ccagtccgaa
       61 gtactcatgc tttatgtctt ctttatcggg catatcggga acaccttcgg aaatgaaacg
      121 caagattgtt ttatcccacc atcccgccca ttcaatcatg ggcaacctgt ccacgggtcg
      181 gaaattcata acatttaaaa atctctctct ttgattcata gaaactcctc aacggttata
      241 ttaccagtcc gtaattgctt agtcaatttg ttttgactgt aataactttt caaattcctc
      301 atatggcatg ggttgataat agtagtagcc ctgagcaata tcacaatcaa gcgaccttag
      361 tatatcggct tgctcttttg tttccacacc ttcgcatatg ataagcgaat ccatattatg
      421 tgcaagatcg gttatgcctt ttatcatctt tttggtttta ctgtcttcta agttgtatat
      481 catgcttctg tcaagtttga gatagtccaa catgaaattg ggcaaatcgg agaaggagga
      541 ataccccgtt ccgaaatcgt ccaatgaaat ggtaaagccc atttctttta gtttattgac
      601 tatatctttc atcatcttcg cattttccgc aataagactt tccgttactt caatgttcag
      661 ataattggga gatacttcgt attgtgttgc tattgcaaat atccggtcgc agaaatcgat
      721 atttgcaaag tgcgaacgtg caaaattaac cgacacggga acaacctgtc tgccttcttt
      781 aagactctgt tgcaacagtt ggcaagcctt ttcaaacatg tacatatcaa atgtcgtaat
      841 aataccgctc ttttcaaaaa caggtatata ttcgcccggc cggataagtc cgcgtctggg
      901 gttataccat ctggacaata tctcgccgcc gtatattgag ttgtcggcaa gcagtatctt
      961 aggctgtata tatatgagga attcgtcttt ttccatgctt gcaaggaatt cctgttgcat
     1021 aagtttttcc tctacggtca taatacgaac ggtatcatca taaaacttgt atctaacatc
     1081 atccttttcc tttacgaact gacgggcctg caaagatttc tgcataagga catccatatt
     1141 cagttcttca tatttaggat tataaatgcc gatactcagc acaacgggat aatcggagtt
     1201 tttaagatcg tacttttcat ttaccttctc tactatattg gataatctcc ctatcatttc
     1261 ctctctttcg gtaaattgaa gcaacaatat gaaatcgtcg ttatttgctc ttgcaagaat
     1321 ctcattgttg taaatatgat tatccagcag tacggctata tcggtctgaa ccttgtccgc
     1381 ctctgtatat ccgtaaacct cccttatgta attcaggtta tacacaacaa gattaaccat
     1441 gcagtatgta cttatattca acatactgat ggaaacatcg gttaccgctt tcatcccctt
     1501 tatattggga taccctgtaa ggctgtcggt attcagaaaa agatttttat tctccttata
     1561 cttgagatag atgataagaa tcatagcaac cgtcagtatt gcgaatataa ctgccgagag
     1621 agcaatcgtg acacgcagtt cggttctgtt gaacattttt tcatactgca cggcctcgct
     1681 tgcgataatg gtctttatat cttcctcgga aatcgtatct atggcacggt taataagtgc
     1741 agcaacannn nnnnnnncat tctttgggta aactgcatcc ccacatcata acctttgaat
     1801 tttaccaact gtctgctttg catgaagccg gcatacttat ttactttcga tacggtaata
     1861 tccgcctgaa agttaaatcc gtactgcagg gcttgttcaa aatcttttgt tttgagatat
     1921 tcgaattgaa atccggtcag ctgatgtaca tggtctaaaa tccgtacgac aacaccttca
     1981 tattgtttct ccgcatagtt gtagaattca aaaggaaaac tatttctgtc cagtgccaca
     2041 tagacaacgg ggtaagacgc cacaacggcc tgttcttcct ttgtcagcac aaaggtttgt
     2101 tcctcggccg catatccgta tattgcagac attaccagaa tacatatgat tgataaaact
     2161 aaaaatctct tcttcatcta ctctcctatg ttcagtttat gtgctttatc acagtccttt
     2221 ttgatgcctt ccgctcccct tatgaagctt ttaaggactt tggataccga ttgcctgaat
     2281 tcctcgaact cttcctctac ctgtttatat tgctcggcct gacgctcttt ttcctccatc
     2341 gtgtctttca gtattatatc ggcctgcttt cttgcatcgg ccaatatatt gtccgccttt
     2401 ttctttgcat gaatcaaaga aagagcaata tcgtccttat tcttctccag catctccaat
     2461 cttttaagaa gcattttatt ctgctccagt ataagattga cctgcctgtc tttttccgct
     2521 atgcgactgt tcagatcttc attcaagagc tctatgtaca taatgacatc tttttttgaa
     2581 tatccgaata aaactttttt aatacctttg aatgcaatca tatctgttat cctttattat
     2641 attcatatac attgtaatat attctccgta ttattggttg aattttaccc tcttaatttt
     2701 accccactgc agttttcttt tcttatagcc tataaaagca gtcattctgg tataggccag
     2761 tattactctg aatccgacat tttcaaatat acaagctcct attgctttcc acacatcctt
     2821 tgccctcagc tctatattct gagtgtgtat cctagagaaa aaggcggaca atgtcaatat
     2881 cgctccgtaa aggagtgata ttgccgtgta tgctatggca acccttgtag tgaccatgcc
     2941 cagaagaaac atggctataa ccgacaatat accgaatatc tctatataag gagatagcaa
     3001 ctcgaagata agaaaataca tgtaggatac cgttctgacc aatccgaatt tactgctgaa
     3061 aagtatctgt ttgtgcttgg tcagactctg atatagtccc agatgccatc ttcttctctg
     3121 ttttctcaga tctctcatgt tatcgggagc ctggctccag cataccgcat cggtggcata
     3181 ctttatggta tacggtattt gattcttaag acaaaacaca tgcaaccgca caaccagttc
     3241 catatcttct cccatggtgg tgtgatcata acctccgacc gcaattacca tatccttttt
     3301 aaataatccg aatgccccgg atattatcag gcttccgttg aacatatcaa acagtattct
     3361 ggatgccaga aaagaccgtt catactccaa aacctgcatt gccgcaacca tattgtccgg
     3421 catttcgtac tttttgactt ttccgttctc cagcaccacc ccgttggcta tccttataac
     3481 tcctccgcat gcaataagat tctcatcggt aagtaccggc cggactattc tctcaagagc
     3541 gtccgactgc agcatggaat cggcgtccat acacacgaaa taaggatatc tcgatgcgtt
     3601 tatacccatg tttaaagcat ccgccttgcc gccgtttttc tttcttatga gtactatggg
     3661 aactttatat gcatagcttt catatatgaa ttcctcgtcc tggcaccgta tgcttttctt
     3721 tatttttctc cgcaccttgg tcattttgaa ctcttcaaca agcagtttgg atgtgttgtc
     3781 ctttgaaccg tcatccacaa ctataatctc atagagcctg taatcaagat ttaaaagaga
     3841 gtgtacagta tcgcaaatgg tgacttcttc gttgtatccg ggtactacga tggatacagg
     3901 aaaatagtaa tcatgggaaa ttacattgtg cagagtactc agctgcttgg ttctgtacag
     3961 ttcggaagct ccgatagtta ccgataccaa taaaaatgta gcatatacaa aaaggtaaag
     4021 agagtaaaaa atccctattg atataagtat tgctcttgct gtttcaataa acatcccgtt
     4081 tctcccttct cgtttttctc ttgagtcttt ccgtttccac tttatactcc agtatttccc
     4141 ttgcgtacct gtccgatccg ttatatatgt ccactaaatc catatatttc acatccatcc
     4201 cgaccagact ttccgatgca ttgaatctca cataccagtt gtccgatgat aatgcctctt
     4261 tcagcgcttc caccacttca tcgcatttat atgccgaaag agcagtggcg gctattgccc
     4321 ggtattccca ctcggaattt ttctgtctca gaagctccac aagatactgt cccgcgggag
     4381 cataagctat ccttccgaag tatctaatta cagcaagcct tatttctttg tgcgagtgtt
     4441 tgtctttcaa caattttagg aacttttcgc tgtaatccag agtagacatc tttatgaagt
     4501 ttactattgc aaccctggtg cttacatcaa agctgccgaa ttccttccac aaatacgatg
     4561 caaggtgtgc tctgtctcca tggaaaagca ttaatccatc ggcaataagt ttattgttgt
     4621 gctttatttt catttcctgc attctcagca ttgcggtcag tatggcttcg ccgttatcgg
     4681 atgtatacaa tgtatgaaga gcattctgtc taacaaacac cgacttattg gtcgtgtacg
     4741 atatcattgc cgagactatg aagtcataga caaattggtc acacagcctg aactcgccga
     4801 taagaaaagc aagataacct tttttagaat tttccttatt atcaaactct ataataagat
     4861 attcaaacag cggtttacac tgtttaagat atgcgaatgt gctctcataa tctacagccg
     4921 cttgctcttt tacaatctca tgaaatgctt ttaagaaacg tatcttagtg aaattcctgt
     4981 acagatactg ttgcttttgt ttgtcaatct cctcattctt acttacaagc tcaatctgat
     5041 cttttattgc ctgcctgatt ttttctcttt tatctttgaa tataaaatcc cggtagcgca
     5101 gatagagaag aaagaccgtg ttgaaacaaa tcatcatcac acagattgcc gtgtatataa
     5161 tcaatatgag atctatcctt acctcattcg ccatccgctg tccgctcctc gtctgtccac
     5221 attttttgta tcatgtaata tgacgcttta agtctttcat gatatgttac cttgttccat
     5281 ccttttaaac tgaacgggaa catttctatt atatgccctc tgttttccgc cgatccgaca
     5341 cctacataca tcttatctat acctatactt tccactccgt aatgctcata atagtcgtca
     5401 tgaataaaca tattggacgg atcggaaaaa ttcagaagat tccagggaat tctcatctca
     5461 atatagttgc cggcaatatg gaaatctgca agcgaattgt attctttatg tgcggggttg
     5521 ctgtttccgt gtgtcaatat tcccgtttca taaacttgcg ccttaatgcc gggatggttg
     5581 actttttcca atgcttccag ctgaagtatg agatttatct tattgaatac cgggctgttt
     5641 atgtgcggag gacgggaatg cggattctct cccgctatat actggttata catagctctc
     5701 agcacatcat atcgttcctg aaccagtacc ctgctatcat ttatcccgtt taatactata
     5761 agaaagtccg catagcagtc aaaccgcaca tcatgttgct tgcagtagtt gctcccggat
     5821 tttcgggtaa tatctatggg tatgtaaaga atatcgcgtt cacggtcata atccttttta
     5881 tatacaaggt aatatatgaa cttttcgtca tactgcattg atacgctcat atcaccgttt
     5941 acggcaaccg gttcaatacc tacccattcg tttatatcac cgtcaacgtg acataatact
     6001 tttttctctc ccgggtcaaa agtcaatatg ccgaatccct gatcggaaga ctgtgcatcc
     6061 gaccagtaca cacaccttct ctcatccacc gaatgttgtg tgttccaggt ggtcagcgac
     6121 cagtcatcct gccacacata cacaaggctt ccggcacaat ccgccgattt tatctgctca
     6181 tatgtattaa ctattatctg tccctgttca tattccgtat tcctgccctg attataccct
     6241 ctgtttgtat cctcatgagc catccctctg gaggtaggca ctccgaactc ggttataact
     6301 atgggaatgc tgtgatggag tttaagcatt ttcagatatg ccgtataggt gttgacatta
     6361 ccttccgcat ccttaaacca aaaatcctta ttttcaaaat agttaaggta accggggtaa
     6421 taagggtaag catgatagga ggcgaaatgt ccggatataa atgcatcggt cgtccttata
     6481 tgctccacat ccacctcggc acactttttg aaaaattccg ttatataatc gggatatacg
     6541 aaaggatcgg tagacggcca gttaaagaaa gataccaacc tctgctcttt ataccttgcc
     6601 gattcgtaag taattatggt atcgcctatc attgcaagca ccgattcgaa tgccgtcgca
     6661 tcttccgtag tgtacatata cttgccgctg tagccttcat gtactttatg caacctgtcg
     6721 gtatatgcga tggtgttgct gttccagttg gagcctaaga catatcccaa tacccagggc
     6781 gagatatctt tcatatagaa tccgtaaccg ctttgctgat taagtaccag ttggcgtttt
     6841 ccgtgtatta tatccacaag aatatatgtc tgctttatta tctctttata aaaatcttca
     6901 tctttcgcat ctatatgact gttctggaca taatcggaaa cggtaaggcc gtgaagaaga
     6961 taaagagggt cttcattatc tttattaaaa tcatacagcg cattatagaa gtctacattc
     7021 attatctgat acacccttat cgtattggcg cccatttctt ttatctgttt taaccacctg
     7081 aggtaatcat cgtattctat ggcataatcg gttccgaatt tgccgggtat ggcacttcgg
     7141 atatttacac ctttaatctg aaatttctca tattgtccgt ttttcagaat atatatgttt
     7201 tcctcatcgg taatgaatga cgtcgtaaca ggtgtattag ggtgcaaatc tataaagaag
     7261 ttcgtggaga acagcacata gtttatacct aatgcaagaa tcaatataat acccgttgct
     7321 atcaaaaact tcttcattta acctctttac atgcctaatt attgaaggac ttgtttctgg
     7381 aatcgtggct gtactgtttt atcgaatcaa tattctcatc catccatttc cctcttgccg
     7441 ttatatagtt cttaagctga ttca
//
LOCUS       DAOPWC010000018         7457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_9908_length_7457_cov_3.708322, whole genome shotgun sequence.
ACCESSION   DAOPWC010000018 DAOPWC010000000
VERSION     DAOPWC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_7457_cov_3.708322"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKJ65_00810"
     CDS             complement(<1..424)
                     /locus_tag="PKJ65_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ40407.1"
                     /translation="MERLIYFENLSDMMSVFGSSDCNLNVIENMMNVSVIVRGSEVKL
                     KGEAEEVNATEKLIYMLIEMAQSGEDINKQNIVYLADLVKNGKDEIYQLPTGYICLNA
                     SGAPIKAKTLGQKKYTDAIKENGITFGIGPAGTGKTFLA"
     gene            complement(510..896)
                     /locus_tag="PKJ65_00815"
     CDS             complement(510..896)
                     /locus_tag="PKJ65_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNZ40408.1"
                     /translation="MSGLTESMENYLRCIHELSYTNKGVRLTDLADKMKVRKASANNA
                     VMKLSKLGYCAQVKYGPIFLTDAGIEAVKAATGKHLIIVKFLKDILMVDEETAEAETT
                     KIEHHLSTSTVYAMHQLHSKLKNRLD"
     gene            complement(1046..2044)
                     /gene="trpS"
                     /locus_tag="PKJ65_00820"
     CDS             complement(1046..2044)
                     /gene="trpS"
                     /locus_tag="PKJ65_00820"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ40409.1"
                     /translation="MKDIVLTGDRPTGPLHIGHYVGSLKKRVEIQEEYDKPFIMIADT
                     QALTDNAHNPKKVSENVYEVAFDYLAVGLKPELNTFFIQSLVPELAEFTVFYMNFVTL
                     ARLKRNPTVKDEMQQKGFGYNVPVGFLNYPISQVADITAFKANLVPVGDDQLPMLEQT
                     NEVVRHINNHFNTAVLRECKPLLSKVLRLPGTDGLSKMGKSMNNAIFLNDEPDVISAK
                     VMSMYTDPNHIRVEDPGNVEINTVFKYLDAFDPEQEELKELKEHYKRGGLGDVKLKKR
                     LNNILQNTLEPIRQRRKEYEENPEEVMKLLKTGSEYARSVAAKTLKELKEVFTLNY"
     gene            complement(2115..3227)
                     /gene="prfB"
                     /locus_tag="PKJ65_00825"
     CDS             complement(2115..3227)
                     /gene="prfB"
                     /locus_tag="PKJ65_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ40410.1"
                     /translation="MIDINDIRNQLEEIKEQSDKLILSLDSDSAREEIAALEKETVKE
                     DFWQDNENSQKVLQRIKVLKDRAAKAADLQTKLDDCFILLEFIDEHPEDEELSNELIE
                     AMEQLNDTYTEIRMYSLLNGTNDKNNAYLTLHAGAGGTEACDWAEMLFRMYTRYFEQK
                     GFKATVTDYLDGDGAGIKSVSVYVEGEYAYGYLKSESGVHRLVRISPFDSAGRRHTSF
                     ASCEVIPEISDEIEVKIKPEDLSVDTFRSSGKGGQHVNKTDSAVRITHLPTNVVVACQ
                     SERSQHQNRDKAMKMLMSKLMEIKEREHKEKIEDLKGNQMDIGWGSQIRSYVFCPYTM
                     VKDHRTGYETGDVQSVMDGNLDAFINEYLTQNPAID"
     gene            complement(3227..4393)
                     /locus_tag="PKJ65_00830"
     CDS             complement(3227..4393)
                     /locus_tag="PKJ65_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ40411.1"
                     /translation="MKKPGEFIREEVRNTPPSGIRKYFDMLSGLSDVISLGVGEPDFV
                     TPWQIRESAIYSLEKGRTHYTSNTGLLELRQAVCNMLRKKYNLDYNPLNQIIITVGAS
                     EAIDIAIRSIAGYGDEVIIPEPCFVAYKGCVNFAGATAVPLQLKEENRFKLTVEQLKE
                     CVTKKTKALILAYPNNPTGAIMTKEELKPIADYLSTKDIVVISDEIYADLTYGTKHFS
                     FAELENMHDKVIYVNGFSKSYSMTGWRLGYVCAHKDIVAEMLKIHQYAIMCAPSFVQY
                     AAIDALEQCETDVEKMRKQYDARRRYIYNGLIEIGIDCFLPEGAFYVFPNIKKTGLTS
                     ERFCDRLLMEHKVLVVPGTAFSSAGEGYFRATYASSLENIKEALKRMERFVNTL"
     gene            complement(4390..4866)
                     /locus_tag="PKJ65_00835"
     CDS             complement(4390..4866)
                     /locus_tag="PKJ65_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNZ40412.1"
                     /translation="MDILELLKQDARLTPKELAVMTGKSVEEVEKVIKQYEQDKVIAG
                     YSAVINWEKARDNIVVAFIEIKVTPERDYGFEEIAKRIYSYEQVKTCYLMSGAFDIAA
                     VVEGSDLKEVALFVAQKLSAIQGVTATSTHFVLKKYKENDLVFEEEEKDRREAIVL"
     gene            complement(4962..5399)
                     /locus_tag="PKJ65_00840"
     CDS             complement(4962..5399)
                     /locus_tag="PKJ65_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNZ40413.1"
                     /translation="MAKKRFRNAMSGYNKDEVNKYIDNMMEQYEAKIAEKEATIEELS
                     KKAAELQLAYDELKSKEDALVKEKAGITKALIKANEMSEQIIKEAKEQAIKEVGELEV
                     RAEEEREKIVDIKRQLAALQASAAKLLEKFVENLDKTIGSDEK"
     gene            complement(5472..6359)
                     /locus_tag="PKJ65_00845"
     CDS             complement(5472..6359)
                     /locus_tag="PKJ65_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ40414.1"
                     /translation="MKKAAVFSDIHGNLDILQKAIDICKAQDVEQYIVLGDMISDGPD
                     SQAVLDTVMNLTEHVVRGNREEYILNYHAGKRKQWDNNYQCESLVKTHKNLTDAGLKY
                     ISGLPDMKRLNLFGLDTLIVHGSHISVGHFILPCWNIDVYMDMHDRYGCSLYLLGHSH
                     QSYHFDLMGSFFINPGPLGIPPAISVYKREDAFSMGIISIQDKQLVEYRPVYIPYNPA
                     KLKEYYLRDIDSYEHTFWMELIADSFFTKQYDSIKFLHMAKQMAKEKGYENVDPIPND
                     IWEDASNKWRKEHKGTGIF"
     gene            complement(6356..7261)
                     /locus_tag="PKJ65_00850"
     CDS             complement(6356..7261)
                     /locus_tag="PKJ65_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40415.1"
                     /translation="MSVHIEKVPYGGWDNCYRISNDEIELTVTSDVGPRIIRYGFIGK
                     ENVLCEKADQMGKTGGDEWRIYGGHRLWHSPEDKVRTYFPDNEAVDVKIIQNGVRISQ
                     PAETTTMIKKDMEITVKDKGTDVYINHILTNCNLWDIELSAWALTVVAPGGLEVVPQE
                     TLDTGLLPNRMISLWPYTDMNDKRVRWGSKYIFLAHDSKAKKAFKIGISNTDGWAAYF
                     NRNNMFVKKFSPKEDMLYPDYGGSMYETYTCDFMTEMETLSPLVLLGPKEKIEHEELW
                     QLFDNIAPPKDEKEMDKIAKEYITG"
BASE COUNT         1849 a         1775 c         1275 g         2558 t
ORIGIN      
        1 cagccaaaaa tgttttaccg gttcctgcag gacctatacc gaaggttata ccgttttctt
       61 ttattgcatc ggtatatttc ttctgcccca gggttttggc tttaatgggt gctcccgaag
      121 cattgagaca tatatagccg gtaggcagtt ggtatatctc gtctttcccg ttttttacaa
      181 ggtcggcaag atatactata ttctgcttgt ttatatcttc ccccgactga gccatttcta
      241 tgagcatgta tataagtttt tcggtagcgt ttacctcttc ggcttcccct ttcagtttca
      301 cttcgcttcc gcgcacaatt acggagacat tcatcatatt ctcgataacg ttcagattgc
      361 aatcggaact gccgaataca ctcatcatat cacttaaatt ttcaaaatat ataagtcttt
      421 ccattaactc ccgcgtatca attaacttaa acctcaaacg gtctgtcacc aaattatatc
      481 atttatatcg gcagtaaatc aaaccgtacc taatccagcc tgtttttcaa ctttgaatgc
      541 agctgatgca tcgcataaac agtgcttgta gacagatgat gctctatctt ggtagtttcc
      601 gcttctgcgg tctcttcatc caccattaag atgtctttca gaaatttcac gattatgagg
      661 tgctttccgg ttgcggcttt tacggcttca atgcccgcat cggtaagaaa tatcggtccg
      721 tattttacct gtgcacagta acccaacttg gacaatttca ttactgcatt atttgcggat
      781 gcttttctga ctttcatctt atccgcaaga tcggtcagtc tcacaccttt attggtatag
      841 gaaagttcgt gtatacatcg taaatagttt tccatggatt ccgtcaatcc gctcatagta
      901 acaccgccct gatatcagaa tttattgatt aacatgattc taccaaatct gccacttatt
      961 agcaactttt aagttgcact tgttatttcc tgctttattt atcctacctt tatttactta
     1021 atctatatag ttaacgataa cctcattaat agttgagtgt aaacacttct tttagttctt
     1081 tcaatgtctt tgcggcaacc gacctggcat actcgcttcc ggttttgagc agtttcataa
     1141 cctcctcggg attctcttca tattcttttc tgcgttgtct tataggctcc agtgtgtttt
     1201 gcagtatatt atttaagcgt ttcttcagtt taacatctcc caatccgcct cttttatagt
     1261 gttctttcaa ttccttcaac tcttcctgct cagggtcaaa agcatcaaga tatttaaaaa
     1321 cggtatttat ctctacattt cccgggtctt ccacgcgaat atgattaggg tcggtataca
     1381 tgctcattac cttcgcgctt ataacatcag gctcatcatt taaaaatata gcattattca
     1441 tggactttcc cattttactt aatccgtcgg taccgggtaa acgtaatact ttggaaagta
     1501 aaggcttgca ctctctcaaa acagcggtat taaaatgatt attaatatgt cttaccactt
     1561 catttgtctg ctccagcata ggcaattggt catcgccgac cggtactaaa ttggctttga
     1621 atgcggtaat atcggcaacc tgactgatag gataattcag aaacccgacc ggaacattat
     1681 atccgaaacc tttctgttgc atttcatcct ttaccgtggg attccttttc aatcgggcta
     1741 atgtaacaaa gttcatataa aataccgtaa attcggcaag ttccggtacc aatgattgta
     1801 taaaaaaagt gtttaattcc ggcttaagtc ctactgcaag ataatcaaat gcaacctcat
     1861 atacattttc acttactttt ttcggattgt gtgcattatc ggtaagcgcc tgtgtatctg
     1921 ctatcattat aaaaggttta tcatattctt cctgtatttc cactcttttt ttaagcgaac
     1981 ctacataatg tcctatgtgc aaaggtccgg ttggacgatc tccagttaaa actatatctt
     2041 tcataaagtg ctcctttcct ctctttgtgt atgctacaca aatacatttt gggcaaacta
     2101 gtacgcttat atcactaatc tatcgcaggg ttctgcgtca gatattcgtt tatgaacgca
     2161 tctaaattgc cgtccataac cgactgcaca tctccggtct cataaccggt cctgtgatct
     2221 tttaccatgg tatacgggca aaaaacataa gaccgtatct ggcttcccca gcctatatcc
     2281 atctgatttc ccttaaggtc ctcaatcttt tccttatgtt ctctttcctt tatttccata
     2341 agtttggaca taagcatctt catggcttta tccctattct gatgctggct cctttccgac
     2401 tgacaggcta caacaacatt ggtgggaaga tgggttatcc tcaccgccga atcggttttg
     2461 ttgacatgtt gtccgccctt tcccgaggaa cggaatgtat ctacggacaa atcttccggc
     2521 tttatcttta cctctatctc atcggaaatc tccggtataa cttcacagga tgcaaaggaa
     2581 gtatgtcttc ttcccgccga atcgaaagga gatattctga ccagtctgtg tacacccgat
     2641 tcgcttttta agtaaccgta agcatattcg ccttccacat aaaccgatac cgattttatg
     2701 cccgctccgt ctccgtcaag ataatcggta acggtggctt taaaaccctt ctgctcgaaa
     2761 tatcttgtat acatcctaaa cagcatctcc gcccagtcac aggcttcggt cccgcccgct
     2821 cccgcatgta atgtcagata tgcgttattt ttatcattgg taccgttaag cagagagtac
     2881 atgcgtattt cggtatatgt atcgtttaat tgctccatag cttcaataag ctcattgctc
     2941 aattcctcat cttcgggatg ctcgtcaatg aactcaagca atataaagca gtcatccagt
     3001 ttggtttgca gatcggctgc ctttgccgca cggtctttca gcactttgat tctttgaagc
     3061 actttttgcg agttctcgtt atcctgccaa aaatcttctt ttacagtctc tttctcaaga
     3121 gctgctatct cttctcttgc gctgtcggaa tccagactca atatgagctt atccgattgc
     3181 tcctttattt cctccaattg atttcttatg tcattaatat caatcattaa agtgtgttta
     3241 caaacctttc cattcgtttt agggcttctt ttatattctc caacgaactg gcgtatgtcg
     3301 cacggaaata gccctctccg gctgaggaaa atgccgttcc cggtactacc agcaccttat
     3361 gttccataag caacctgtcg cagaaccgct cggatgtcag tccggtcttt ttgatgttgg
     3421 ggaatacata aaaggcacct tccggtaaaa aacaatcaat acctatttca ataagtccgt
     3481 tgtatatata tcttcttctt gcatcatact gttttcgcat cttttccaca tcggtctcac
     3541 actgttccag agcatctatt gcggcatact gtacaaatga cggagcacac attatagcat
     3601 actgatgtat tttgagcatc tcggcgacta tatctttgtg tgcgcataca tatcccagtc
     3661 tccagcccgt catggaatat gatttggaaa atccgtttac atatattact ttgtcgtgca
     3721 tgttttccaa ctcggcaaag gagaaatgct ttgttccgta tgtgaggtcg gcatatatct
     3781 cgtcggatat gacaactata tcttttgtcg acagataatc ggctatgggc ttcaactctt
     3841 cctttgtcat gatggcaccg gtcggattgt tggggtatgc aagaatgagt gctttggttt
     3901 ttttcgttac acactccttt aactgctcca ctgtcagctt aaaacggttt tcctctttta
     3961 actgtaacgg taccgccgtt gctccggcaa agtttacaca tcctttatat gccacaaaac
     4021 acggctccgg aataataact tcatcaccat atcccgctat gctccttatg gctatatcta
     4081 tggcttccga tgcccctacc gtgattatta tctggtttaa gggattgtaa tccagattat
     4141 acttttttct cagcatatta catacggctt gtctgagctc taataacccc gtattggatg
     4201 tgtagtgagt tctgcctttt tccaatgaat atatggcgga ttctctgatc tgccacggcg
     4261 taacaaagtc cggctccccc actcctaaag atatgacatc actcaatccg ctaagcatgt
     4321 cgaaatattt tcttataccg gaaggaggag tgtttctcac ctcttccctt ataaattctc
     4381 cgggtttctt cacagaacta tagcctctct tctgtctttt tcctcttctt cgaatacaag
     4441 gtcgttttcc ttatacttct taagcacgaa atgcgtggat gtagcggtta ctccctgtat
     4501 ggcggaaagc ttctgagcca caaacaatgc cacttctttc aaatcggacc cttccacaac
     4561 cgccgctata tcaaaagctc cgctcatgag gtaacatgtt ttcacctgct catagctgta
     4621 tattcttttt gctatttctt caaatccgta gtctcgctcc ggagttacct ttatctcgat
     4681 aaaagccaca actatattat cccgtgcttt ttcccagtta ataacggcgg aataacctgc
     4741 aattaccttg tcttgctcat actgctttat tactttttcc acttcttcaa cgcttttacc
     4801 cgtcatgacc gcaagctctt tcggggtaag ccttgcatcc tgttttaata attccagtat
     4861 atccataact tttcctttct tataaaaaga ggactgacag tcctctgaga ataccgggtt
     4921 tcaagcaacc ggctattctc ttaatctgat gtattttaca gtcatttctc gtcggaaccg
     4981 atagttttat ccagattttc cacaaacttt tccaaaagtt ttgctgcaga tgcctgcaat
     5041 gcggccagct gcctttttat atccactatc ttttctcttt cttcttccgc tcttacttcc
     5101 agttcgccca cttccttaat agcctgttcc ttggcttcct ttataatctg ttcggacatt
     5161 tcattggctt ttatcaaggc ttttgtaatg ccggcttttt cctttacaag agcatcttct
     5221 ttactcttca attcatcata tgcaagctgc aactccgccg ctttcttgct gagttcctcg
     5281 atggtggctt ctttttcggc tattttggcc tcatattgtt ccatcatatt gtctatatac
     5341 ttgttgactt catccttgtt gtagccgctc attgcgttac ggaatctttt ttttgccatg
     5401 tcacacctct tctaatgatg atatttatct cgttttaatt ttatatataa tcagcccgaa
     5461 tagcaagcat tttagaatat acctgtacct ttatgctctt ttctccattt gtttgatgca
     5521 tcttcccaaa tatcgttggg tatcgggtcg acattttcat atcctttctc ttttgccatt
     5581 tgcttagcca tatgtaaaaa tttaatactg tcatactgct tggtaaagaa agaatcggct
     5641 ataagttcca tccagaaagt atgctcataa ctgtcaatat ctcttaagta atactcctta
     5701 agcttggcag gattatacgg tatgtataca ggtcggtact caacgagttg tttatcctgt
     5761 atcgatatta tccccatact gaatgcatcc tctcttttgt aaacactgat tgcgggcggt
     5821 atccctaaag gtccgggatt tataaaaaag ctgcccatga ggtcaaaatg ataggactga
     5881 tgcgaatgac ctaaaagata cagtgagcag ccgtacctgt catgcatatc catatataca
     5941 tcaatgttcc aacaaggcag tatgaaatgt cctacactta tatgagagcc gtgtacgata
     6001 agggtgtcca gaccgaaaag attcagtctt ttcatatcgg gcaatcccga aatatactta
     6061 agtcccgcgt cggtaagatt cttgtgcgtc tttaccaacg attcgcactg atagttgtta
     6121 tcccactgtt ttcttttacc ggcatgataa ttcaagatat attcttcccg gttacctctt
     6181 acaacatgct cggtaagatt catgacggta tccaacactg cctgcgaatc gggaccgtcg
     6241 gaaatcatat ctccgagcac tatatattgc tcgacatcct gtgctttaca tatgtcaatt
     6301 gctttctgaa gtatgtcaag attgccgtgt atatcggaaa aaactgccgc ttttttcatc
     6361 cggtaatata ttctttcgct attttatcca tttctttttc gtctttagga ggagcaatgt
     6421 tgtcaaacag ctgccacaat tcttcgtgtt caatcttttc tttgggaccc aacagaacaa
     6481 ggggtgaaag ggtttccatt tcggtcatga aatcgcaagt ataggtctca tacatagatc
     6541 cgccgtaatc gggataaagc atgtcttctt taggcgaaaa ctttttcaca aacatattgt
     6601 tccggttgaa atatgccgcc caaccgtcgg tattggatat gcctatttta aaagcctttt
     6661 ttgcttttga atcatgtgca aggaaaatat acttacttcc ccaacgcact cttttatcat
     6721 tcatatcggt atacggccat aaagatatca ttctgttggg taaaagaccg gtatccagtg
     6781 tttcctgcgg taccacttcc agtcctccgg gtgcaactac agtcagcgcc catgccgaaa
     6841 gctctatatc ccacagatta cagtttgtta atatgtggtt gatatataca tcggtccctt
     6901 tatcctttac ggtaatttcc atatcttttt ttatcattgt ggttgtttcc gcaggttgcg
     6961 atatccttac accgttttgt attatcttta catccacagc ttcattatcg ggaaaatagg
     7021 tgcgcacttt atcttcggga ctgtgccaca gcctgtgtcc tccgtatatt ctccactcat
     7081 ctcctccggt cttacccatc tggtcggctt tttcacacaa gacattttct ttgcctataa
     7141 atccgtaccg gatgattctc ggaccgacat ccgatgtaac tgtcaactca atttcatcgt
     7201 tacttattct gtaacaatta tcccaaccgc cataagggac tttctcaata tgtaccgaca
     7261 ttttttcacc tcgtattttt atataactgt tatactatgc ctttttaagg caatgtcaga
     7321 ttgtgcgata tatagtaacc tgtattattt cttttttccg gtaaaccagt gaattatagg
     7381 tataaccagg aatattatcc aacccggatg ccataaatcc catataaatc ccatagccag
     7441 atatattaag actatga
//
LOCUS       DAOPWC010000019         7380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_10042_length_7380_cov_2.895154, whole genome shotgun sequence.
ACCESSION   DAOPWC010000019 DAOPWC010000000
VERSION     DAOPWC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10042_length_7380_cov_2.895154"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKJ65_00855"
     CDS             complement(<1..246)
                     /locus_tag="PKJ65_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015165597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNZ40416.1"
                     /translation="MMGRYRLITRSDFDGLVCAMILKELDMLEDIMFVHPKDMQDGMI
                     DVNERDISTNVPYLPGVYLAFDHHISEKGTKAVNYINK"
     gene            complement(330..1925)
                     /locus_tag="PKJ65_00860"
     CDS             complement(330..1925)
                     /locus_tag="PKJ65_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNZ40417.1"
                     /translation="MKKIFELILSNIKEAMSKFVVSFYGSVIAFILLTLQIIFDFTEN
                     TVVLMAFSVGFAVIVSVMMQIISIKYNFSNKADIIQRIVSALTAVPCYFLIGNIMESD
                     RTFMGFFGLIIAFLLISLYVMYDNENSDLVFPQIIKSAFFAVTVASLFFGGITLCILA
                     LDFLVVELQQFDKWITITALLSYIILCYNLFIAMLPEKTDEIVLPKIFKVLVLYVGLP
                     VYTLLLGVLYAYLIKILVIMEMPQGKINLFASLASLFFIFFYLTVGSYDNAPVRFFRK
                     YGGWFLFPIIASQAIAIYIRVNAYGLTSARWASILLNVTALVFIILTLIKEGKYTKHV
                     ITGLAVIVLISSLTPLNIIDVPVYEQTYRLEKVLKRNNMLADGTVKPYKDISKTDKEI
                     ISSCYDYLNYKDKAPEYIKESNSFYDIFGFEKLNEYGSLDEYSLIHLNKSNSYNALDI
                     SGYSRYYIVDTSRERELKIVIDNTEYNVAEQIMKIKEGNDRSDMIFDVTEDIRVYLTY
                     VYYEMNNEGEVLYFSATGFALGK"
     gene            complement(1971..3857)
                     /locus_tag="PKJ65_00865"
     CDS             complement(1971..3857)
                     /locus_tag="PKJ65_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ40418.1"
                     /translation="MDFKKRAAELVSKMTLAEKMSQMQFESPAIERLGIPEYCWWNEA
                     LHGVARSGTATVFPQSIAMATSFNDRLMYKVATAISDEVRAKYNQYRKLNGTKIYEGL
                     TCWSPNINIFRDPRWGRGHETYGEDPLLTAKMGTAFVKGLQGNDKVYRKTDATLKHYA
                     VHSGPEWLRHSFDAHVSETDFYETYLYAFEYCIKHAKPAAVMGAYNRVYGEPCCGSPF
                     LLQKILRKKLGFKGYVVSDCGAICDFHLHHKVTENEAQSAALAVKSGCDLNCGDAYKW
                     LKVAVAEGLIKEKDITKCVERLMYTRLKLGMFDKECKYNDIPYSVICSKKHKKLNLEM
                     ARESIVLLKNNGILPLKKNTNVAVVGPNADDKSVLLANYNGTPDDYYTLLRGIQSVCK
                     GDVLYARGSHVFEDESTWRYSEHPLREAIITAGYSDVIIMCMGINPSIEGEESTHRGF
                     KGDRDEIELPAVQYKLFDELKKLGKPIIFVNVSGSCVGLAHADKNADAVMQCFYPGAL
                     GGIALADVIFGNVSPCGRLPVTFYEKTSDLPDFEDYSMKGRTYRFFEGQPVYRFGHGL
                     TYANIKEEWTDAYTVKLTNLSDVDTGYSVLRYEDKTKRKLLDFKKVQLKGNSSITVQF
                     APEK"
     gene            4084..4737
                     /locus_tag="PKJ65_00870"
     CDS             4084..4737
                     /locus_tag="PKJ65_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ40419.1"
                     /translation="MSEISNLFPVWYHLTEEQKQLLNDKVYIKDVKKGTVLHRGDEDC
                     DGLYIVRTGQLRAYIISDQGKELTLYRLFEMDICLFSASCILSNIQFEIIVEAEKDTS
                     ILIIPVRVYQKLMETNNAVANYTNHLMASRFTDVMWLIDQVLFKSMATRLASFLLYES
                     NIEESDELNITHEKIANHIGTAREVVTRMLKYFQQEELIEMARGSIIITDRKKLAEL"
     gene            4972..7134
                     /locus_tag="PKJ65_00875"
     CDS             4972..7134
                     /locus_tag="PKJ65_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNZ40420.1"
                     /translation="MFCTNCGKQIPENSNFCTYCGASTGNIPQPMQNQQQFRQPYQQP
                     FNQYPQKPKKGKGGVVALVAVLIIALIVTGIFVVPRIIDYFKSSPPSGKFASIGFTEQ
                     NVSVEAAKIVDNRGGVISLPSDSALAGCYVTVPATAVDQHINVSVGTVTGTYKNAPAS
                     ISSTALHLDLGGYKDINQPILITFKYADTPAEAEKVPVGLYIDEAGKLHHMLTHSIDK
                     KAGQFTVMTFHASTFTYYILEDMDVYPDSSTTGFMPSKDGFAIVNHGSSLFSGGECYG
                     MSTFAKWYYMNKKTSASDALYNLFKTPQMGVTPKGDAITPQDIIATKAFQYTTKQSSV
                     LYDTERQFSSYVETDEDGKIIKYTADNSVAVRCIMDSLYFWEEPVEVGIYGTAGHSVL
                     AYGYTKTADKITIKIYDPNFPGDNNQKIVYDIATKKISTPAYADGFLDKRLTTTGSGT
                     FTSVNEYEKILIDAKSNFAGTIADLVVIDPYNGDEVEERTYVITGYLDTLGMMGEQIG
                     DAVEIISDEGEIFRQNLSKDGSNASTFSIEVPLKSGENKFLINAIYKDEKGNENFLSH
                     NLYGWLVINCNAAANAIYITLTWDSQPDIDLYVSTPYGETAYWESKITSDGGFLDIDQ
                     TYGYGPEHYTLVASDSVAWGQAYTVRLHYFDGDGPTAYKVMVTENEGTKNERTTYYSG
                     VIYTHGFEIDDYYNNYKPGCSGPDGVDICTVVPVRESD"
BASE COUNT         2219 a         1602 c         1402 g         2157 t
ORIGIN      
        1 tttatttatg taattaacgg ctttggtccc cttctcggat atatgatgat caaatgccag
       61 ataaacaccc ggcagatacg gcacattggt ggaaatatct ctttcgttaa catcaatcat
      121 accgtcctgc atatccttcg gatgcacaaa cattatgtct tccaacatat ccagttcctt
      181 taaaatcatg gcacacacca atccgtcaaa gtccgatctc gttattaacc tgtatctgcc
      241 catcatctac ctcctgtcaa tcattcgtac agcacaaatt acatgtttat tatataacaa
      301 aaaactaaaa gtcaagtttt aatataccac tatttaccca aagcaaagcc cgttgcactg
      361 aagtacagca cttccccttc attattcatc tcatagtata catatgtcaa atataccctt
      421 atgtcttctg taacatcgaa tatcatatcc gatctgtcat taccttcttt tatcttcata
      481 atctgttcgg caacattgta ttccgtattg tcaataacta tcttcaactc tctttcccgt
      541 gacgtatcga caatatagta gcgactgtat cccgatatat ccaatgcgtt ataactgttg
      601 ctcttgttaa gatgtatcag gctgtattca tccaaactgc cgtactcgtt aagtttttca
      661 aatccgaaaa tatcgtagaa agaatttgac tcttttatat attcgggcgc cttatcttta
      721 tagttgagat agtcatagca gctggatatt atctccttat cggtctttga tatatcctta
      781 tagggcttaa ccgtaccgtc cgcaagcatg ttgtttcttt tcagtacctt ttccagtctg
      841 tatgtctgct catacaccgg cacatcgatt atattcaacg gagtcaatga cgaaatcaac
      901 actatcacgg ccagaccggt tattacatgc tttgtatatt ttccctcttt gataagggta
      961 aggattataa agaccagagc agttacattc agcagtattg atgcccatct ggcagaggtc
     1021 agtccgtatg cgtttaccct tatatatatc gctatggcct gtgaggcaat tatcgggaac
     1081 aggaaccatc ctccgtattt tctgaaaaac cgcacaggag cattatcgta acttcctacg
     1141 gtaaggtaga agaaaataaa gaaaagagaa gccagcgaag caaaaagatt aatcttaccc
     1201 tgcggcatct ccattattac aagtatttta ataagatatg cataaagcac tccgagcaac
     1261 agcgtatata cgggaagacc gacatacagc accagaactt taaatatctt gggaagtact
     1321 atttcatcgg ttttttccgg tagcattgca atgaaaaggt tatagcagag tatgatatat
     1381 gacaaaaggg cggtaatggt aatccatttg tcaaactgct gcaactccac caccagaaaa
     1441 tccagagcca gtatgcacag cgttatgcct ccgaaaaaca gtgaagccac cgttacggca
     1501 aagaatgccg attttataat ctgaggaaat accagatcac tattctcgtt atcatacatc
     1561 acatataacg atataagaag aaatgctatt atcagcccga aaaaccccat aaaagtcctg
     1621 tccgactcca ttatattgcc gataagaaaa tagcacggta cagcggtcaa tgcagaaaca
     1681 atgcgctgta tgatatcggc tttattggaa aaattgtact ttatgcttat aatctgcatc
     1741 attaccgaca ctataacggc aaaacccacc gaaaaagcca taagcactac ggtattttcc
     1801 gtgaagtcga atataatctg caatgtcaga agtataaaag cgataacgct tccgtaaaaa
     1861 gacaccacga atttcgacat agcttcttta atattactca gtatcagttc aaaaattttc
     1921 ttcataacac cgctcctcgt ccgatacttt aataagtatc ggtctttttg ttatttttcg
     1981 ggtgcaaact gcacagtaat actgctgttt ccctttaatt ggactttttt aaagtctaaa
     2041 agtttcctct ttgtcttatc ttcatatctc aacaccgaat aaccggtatc aacatcactc
     2101 aggtttgtca gttttaccgt atatgcatcg gtccactctt cttttatatt tgcatatgta
     2161 agaccgtgtc cgaaccggta taccggctgc ccttcaaaga atctgtaggt tcttcccttc
     2221 atggaataat cttcaaaatc gggaagatcc gatgtctttt cgtagaaagt aacgggcaac
     2281 cttccgcagg gagatacatt tccgaatatc acatcggcaa gagctattcc tcccaatgct
     2341 cccggataga aacactgcat aacggcgtcg gcatttttat cggcatgtgc caatcctacg
     2401 caactgcccg atacatttac gaaaattata ggttttccaa gtttcttcag ctcgtcaaac
     2461 agtttatact gtaccgccgg taactctatt tcgtctctgt ctcccttgaa tcctctgtgt
     2521 gttgattctt ctccttctat cgagggattg atacccatgc acataatgat tacatcgctg
     2581 taaccggctg ttattattgc ctcacgcagc ggatgttcgg aatatctcca tgtggattcg
     2641 tcttcgaaaa catgcgagcc ccttgcataa agaacatcgc ctttgcacac cgattgtatt
     2701 ccgcgcagca atgtgtaata gtcatcgggc gtaccgttgt aattggctaa aagcaccgat
     2761 ttgtcgtcgg cattgggccc tactacagca acgttggtat tctttttcaa aggaagtatt
     2821 ccgttattct tcaacagtac aatactttct ctcgccattt caagatttaa tttcttatgc
     2881 tttttactgc atataaccga ataaggaata tcgttatact tacactcttt atcgaacata
     2941 cccagtttga gacgtgtata catcagtctc tccacacatt ttgtaatatc tttttcctta
     3001 ataagacctt cggctaccgc cactttaagc catttatatg catcaccgca gtttaaatcg
     3061 catccgcttt ttacagccaa tgccgcactt tgcgcttcat tttccgttac cttatgatgc
     3121 agatggaaat cgcatattgc tccgcagtcg gataccacat atcctttaaa gcctaacttc
     3181 ttacgcaaaa tcttctgtaa aaggaacgga ctgccgcaac acggctctcc gtataccctg
     3241 ttatatgctc ccataaccgc agccggtttg gcatgtttaa tgcagtattc aaaagcatat
     3301 agatatgttt cgtaaaaatc cgtctccgat acatgtgcat cgaaactgtg acgtaaccac
     3361 tcgggaccgc tgtgaaccgc ataatgttta agcgtggcat cggtctttct gtatacttta
     3421 tcgttgccct gcagaccttt gacaaaggcg gtacccattt tagcggtaag cagagggtct
     3481 tctccgtatg tctcatgccc tcttccccac ctgggatcac ggaatatatt gatattgggc
     3541 gaccagcagg tcagtccttc atatatcttg gtgccgttca actttctgta ttgattgtat
     3601 ttggcgcgca cttcatcgga tattgccgtt gccactttat acatcagtct atcgttaaaa
     3661 gatgtggcca ttgctatgga ctgaggaaat accgttgccg ttccgcttct ggcaactccg
     3721 tgtaacgctt cgttccacca gcagtattcg ggaatgccca acctttcgat tgcaggtgac
     3781 tcaaactgca tctgtgacat tttttccgcc aaagtcatct tgcttacaag ttcagcagct
     3841 cttttcttga aatccatctg ttcctcctta tacaaaacaa gatttatgag tttgtaataa
     3901 taaacggtgc acaggcaacc gatgcagtta ttatatcaga agaataccac aattaaaacc
     3961 tgtatatata acatagtttt gttaattctt ataatatagc cggacaaaat tataaataaa
     4021 actttacatg cgtgtatggt ataatcggaa tgtgaacgta taagaatgca tcagggagtt
     4081 atcatgagtg aaataagtaa cctgttcccc gtctggtacc atcttaccga ggagcagaag
     4141 cagttgttaa acgataaggt gtatataaag gatgttaaaa aaggcactgt tcttcataga
     4201 ggcgatgaag actgtgacgg attatacata gtacgaaccg gtcagttaag agcatatatc
     4261 atttccgacc agggtaagga acttactctt tatcggttgt ttgaaatgga tatatgtctg
     4321 ttttccgcat cctgtatact gtccaacata caattcgaaa ttatagtgga agcggaaaaa
     4381 gatacttcca tattgataat accggttcgg gtatatcaga agctcatgga aacaaataat
     4441 gcggttgcaa actataccaa tcatcttatg gcttccagat tcaccgatgt catgtggttg
     4501 atagatcagg ttcttttcaa gagcatggca acacgtcttg cctcttttct tctgtatgaa
     4561 agcaatatag aggaaagtga cgagttgaat ataacgcatg agaaaattgc caaccatata
     4621 ggtaccgcca gagaagttgt gaccaggatg ctgaaatatt tccagcagga agaattaata
     4681 gaaatggcaa gaggcagtat tatcataacc gacagaaaga aactggcgga actgtaaaac
     4741 ggttgccgtt tatatactca atttaaactg catctaaaac ctttcgtact ctttttattt
     4801 aaaaaatatt ttttaaaatt ttatcacggg atattgacgg ccaaatacgg taataataaa
     4861 atatggtaag tatattgtac ataataatat atgaatgtct gtttgttgtg ccgtaccggg
     4921 catttcagat aaatgctctg tcttacgaaa taaatttacg gaggtgaaaa gatgttttgt
     4981 acaaattgcg gtaagcagat acccgaaaac tcaaatttct gcacttattg cggtgcttca
     5041 acgggaaata ttccgcagcc catgcaaaat caacagcaat tccgacaacc ttatcagcaa
     5101 cctttcaatc aatatccgca aaaacctaaa aaaggtaaag gcggtgttgt tgctttggtt
     5161 gccgtactga taatcgcttt gattgttacg ggtatttttg ttgtaccgag aataatagat
     5221 tattttaagt cttctccgcc atccggcaag ttcgcatcca tcggatttac cgagcagaac
     5281 gtgtcggtag aagctgcaaa gattgtggat aacagaggag gtgtaatatc attaccgtcg
     5341 gacagcgccc ttgcaggctg ttatgtcacg gttcccgcaa cggccgttga tcagcatatt
     5401 aatgtttcgg tgggaacggt aaccggtact tataagaatg ctcccgcgtc catttcctct
     5461 acagcccttc atttggattt gggaggctat aaggatatta atcaaccgat acttatcaca
     5521 ttcaaatatg cagatactcc tgcggaagcg gaaaaggtac cggtcggctt atatatagat
     5581 gaagcaggca aactgcatca tatgctgact cacagcatag ataagaaagc cggacagttc
     5641 accgtcatga ctttccatgc aagcactttc acctattata ttctggaaga tatggacgta
     5701 tatcctgatt cctccactac cggttttatg cccagcaaag acggctttgc catagttaat
     5761 cacggatcca gtctgttttc gggcggtgaa tgttacggca tgagtacatt tgccaaatgg
     5821 tattatatga ataagaaaac atcggcatcg gatgcacttt ataatctttt caaaacacct
     5881 cagatgggtg ttacacccaa gggtgacgca ataactcctc aggatattat cgcaacaaag
     5941 gcattccagt ataccaccaa gcaatcttcc gtcctgtatg ataccgaaag acagttctca
     6001 agctatgttg aaaccgatga agacggcaaa attatcaaat atacagccga taactcggtt
     6061 gctgtacgtt gtatcatgga ttctctctat ttctgggaag aaccggtaga agtgggtatc
     6121 tacggaaccg ccggccattc ggtgcttgct tacggctaca ctaaaaccgc tgacaaaata
     6181 accattaaga tatatgaccc aaactttccc ggagacaata atcaaaaaat agtttatgac
     6241 atagcaacca aaaaaatatc cacacctgct tatgccgacg gtttcctcga taaaaggctt
     6301 accacaaccg gtagcggcac atttaccagt gtaaatgaat atgaaaagat acttatagat
     6361 gcaaaaagca actttgcagg taccatagcg gaccttgtgg ttatagaccc gtataacgga
     6421 gatgaggtgg aagagagaac atatgtgata accggttatc tggatacttt gggaatgatg
     6481 ggagagcaga taggagatgc agtggagatt atatcggatg aaggagaaat attccgccaa
     6541 aatttgagta aagacggctc caatgcctct actttctcca ttgaagtacc tttaaagagc
     6601 ggagaaaaca aattcttaat taatgccatc tacaaggacg aaaaaggaaa tgaaaatttc
     6661 ctgtcacaca atctctacgg ctggcttgtc attaactgca atgccgcagc aaacgccatt
     6721 tatataaccc tcacatggga ctctcaacca gatattgatc tttatgtatc aaccccttac
     6781 ggagagactg cttattggga atccaaaatc acatccgacg gagggtttct ggatattgat
     6841 cagacctacg gctacggtcc cgagcactac accttagtcg catccgacag tgttgcctgg
     6901 ggacaggcat atacagtacg tctgcactat tttgacggtg acggtcctac cgcctataaa
     6961 gtcatggtta ccgagaatga aggtaccaaa aatgaaagaa ccacatatta cagcggagta
     7021 atatacaccc acggttttga aatagatgat tattacaata actataagcc gggatgttca
     7081 ggacccgacg gggtggatat atgcacggtt gtacccgttc gggagagcga ctgatgccgg
     7141 caacagtaag aaaaaattaa agttttcata tacatacatc cggatattct ccggatgtat
     7201 gttctttaac cgtattttaa catacatatt attgacaaac cgacatgtta ttctatataa
     7261 ttacagataa attattactt ggtaataaat actgatgagt tatatcaaaa aaagggggaa
     7321 aacattgatc ggtaaacaat catcagttat tgccatatgc aatggtaccg ggttttatgg
//
LOCUS       DAOPWC010000020         7308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_10164_length_7308_cov_4.691300, whole genome shotgun sequence.
ACCESSION   DAOPWC010000020 DAOPWC010000000
VERSION     DAOPWC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10164_length_7308_cov_4.691300"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKJ65_00880"
     CDS             <1..459
                     /locus_tag="PKJ65_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNZ40421.1"
                     /translation="FSVYLLGASHDSVSTAIKNIRLRYPHITIAGFHDGFFSTDQEKE
                     IIDDINAKKPDLIIVGLGAPKQEFFLYKHRYNIHSGLAIGAGGTIDIISGFSRRAPEF
                     FCKHKLEWFYRLLKQPARFRRMLNIPLFLIKCRQHARKTKKAYKKKNRRT"
     gene            complement(565..1263)
                     /gene="rsmG"
                     /locus_tag="PKJ65_00885"
     CDS             complement(565..1263)
                     /gene="rsmG"
                     /locus_tag="PKJ65_00885"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNZ40422.1"
                     /translation="MKQLLKGIFEELRINYREECYEKFDEYASLLIEWNKKFNLTGIT
                     GLRDVYIKHFGDSLLLYEGIEATQSLIDVGSGAGFPGIPLKIAGYKQKIVLLEANGKK
                     VSFLEHVIKTLDLDNIFVCNERAENAAREEVYREQFDVAAARAVAPLNILLEYCVPFI
                     KREGIFIAMKTDNSELQSAENAIKQLSVRILKEDKQTIPFSDIKRNNIYFKKTRNTAY
                     KYPRPDGVPKKKPL"
     gene            complement(1260..3140)
                     /gene="mnmG"
                     /locus_tag="PKJ65_00890"
     CDS             complement(1260..3140)
                     /gene="mnmG"
                     /locus_tag="PKJ65_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNZ40423.1"
                     /translation="MKQYDVIVVGAGHAGCEAALAGARMGLKTAIFTLNLDSIANMPC
                     NPSIGGTAKGHLVREIDALGGQIGKCADATLIQSKMLNKSGGPAVYSLRAQIDRRQYQ
                     TVMKHILEKQENLDIRQYEVTDVVFDNDNRVTGVVTNTDVIISAKAVIIATGTYMKSR
                     IIIGDMSYEGGPDGFFPANKLSLNLEKKGIILRRFKTGTPARVHKNSIDLSKMSVQDG
                     DEPIVPFSFETDKIEIDQIPCYLTYTNEKTHDIIRRNLDRSPLYSGKISGTGVRYCPS
                     IEDKIVKFADKERHQVFLEPMGLMTDEMYVQGMSSSMPEDVQEEMYKSIEGMENVVFM
                     RSAYAIEYDCFDPLQLLPTLEFKKFPGLYSAGQSNGSSGYEEAAAQGIMAGINAVLKI
                     KKRQPFVLDRSEAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLLLRQDNADERLTPKGY
                     ELGLIGKERYTRFLSKMGNIEKETDRLKKKNISPKIINPFLEKRNQQPVNSGTTEAAI
                     LKREGIGYEDLYEIDSEMPELTKEEKEQVEIKIKYEGYIEKQQRQVEQFKKMEKVLLP
                     EDIEYKKIKGLRNEAQQKLDSVKPVSLGMAGRISGVSPADVQVLYVYIEQQKRKKRKT
                     KE"
     gene            complement(3142..4512)
                     /gene="mnmE"
                     /locus_tag="PKJ65_00895"
     CDS             complement(3142..4512)
                     /gene="mnmE"
                     /locus_tag="PKJ65_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNZ40424.1"
                     /translation="MEHTIAAISTPVGVGGISVIRISGSNAFSVAESMFKSKTPFKDI
                     KSHTVTYGHIQDKKGNTLDNVLLLKMKAPKTYTKEDTVEIHCHGGLYVTRKILALAYA
                     FGAAPAQPGEFTKRAFINGSIDLMEAEAVMDLINSQAEDAYKEALSQIKGEKSLQIKS
                     IRSELIDLISHIMVVMEYPEYDTEKITVKEVQDKALNIADRLKVFVEDFKERRIIREG
                     LTVAVIGDVNVGKSTFINRACGQNRVIVTDIAGTTRDIVEVKIDVKGLCVTLKDTAGI
                     RSTDNEIEKIGIEKAKQAIEQSDAVIYIADINQPYKDEIFHYLRDRDKVVCVLNKTDL
                     IQEKDREKHIESMEKELRKLFKDRIIACSLDKKEGINEIFDKFLDMVNAGSIRSKDSA
                     ITNERHKKHILDAITNLENTKEGSFIDMLSIDLTAAAEDLGKITGETASADIIDRIFE
                     KFCIGK"
     gene            complement(4515..5165)
                     /gene="jag"
                     /locus_tag="PKJ65_00900"
     CDS             complement(4515..5165)
                     /gene="jag"
                     /locus_tag="PKJ65_00900"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNZ40425.1"
                     /translation="MSRVSIEKTGKTVDEAVQAALSELGLELGDVEIEIVEEETKGLL
                     GLGRKEAKVRVYFSTEDDPKETIKFFLEDVLSQMNLAPDVFVSEEDGFYKAEISCSDS
                     GLIIGRKGEVLQALQFITSQVVSRAYDTYIRVIIDTENYRERHKAYLEELARKTADKV
                     IKAKRDFMLDPMSSYDRRIIHTCLQNRENISTYSIGDEPYRKIVISFERDGRRNNY"
     gene            complement(5162..6157)
                     /locus_tag="PKJ65_00905"
     CDS             complement(5162..6157)
                     /locus_tag="PKJ65_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNZ40426.1"
                     /translation="MFDFIYNLFGKIFELLYTGLCGQNFGITIIVYTLLLKLLLMPLS
                     IKGQKSTMRMTELTPKVDAIKRRYKNDQQKQNEEIQKLYKQENVGCASGCLPTLIQFP
                     LLIAMYRVLAQPLTYIKGVSKDTINLIIERLGYAPTILQTTINNDLISDPAKFKLVKD
                     LISFDKLIDMNFLGMNLGLVPKVNPSILFGAETIGTYLPILFLPLISVGLVILSQYLT
                     DKAMKKRMPAKKKAPNPNDAPDMSEQMGKTMKFLMPAMTLWIGFTLPVSMSLYWIFTH
                     IVQIVQHFIMERIRKNHDAKKEAEAVRSGAASQGEEVIEATYEVKEESKESNDKK"
     gene            complement(6172..6381)
                     /gene="yidD"
                     /locus_tag="PKJ65_00910"
     CDS             complement(6172..6381)
                     /gene="yidD"
                     /locus_tag="PKJ65_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNZ40427.1"
                     /translation="MKKLIIRLIKFYRINISPNKLPCCRFVPTCSQYAIEAVEKHGAF
                     KGSLLSLWRILRCNPLFKGGYDPVK"
     gene            complement(6353..6712)
                     /gene="rnpA"
                     /locus_tag="PKJ65_00915"
     CDS             complement(6353..6712)
                     /gene="rnpA"
                     /locus_tag="PKJ65_00915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNZ40428.1"
                     /translation="METLKKNYEFLRVYRKGKYFSGKYLTINVLENACLRNRVGISVS
                     SKAYKSSVKRNLYRRRIKEILRTGNFNLKNGYDMVFVVKKTDEIPEYHEIEKEIVDLL
                     DKAKLIIKNEKTDNQID"
     gene            complement(6753..6887)
                     /gene="rpmH"
                     /locus_tag="PKJ65_00920"
     CDS             complement(6753..6887)
                     /gene="rpmH"
                     /locus_tag="PKJ65_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNZ40429.1"
                     /translation="MKRTYQPKTKQRSRVHGFRQRMKTAAGRKIIKNRRARGRKRLSA
                     "
BASE COUNT         1849 a         1653 c         1219 g         2587 t
ORIGIN      
        1 ttttccgttt atctgcttgg tgcatcacat gattccgtaa gtaccgcaat taagaatatt
       61 cgattacgtt acccgcatat aacaatagcg ggtttccatg acggcttttt tagtaccgac
      121 caggaaaaag agataattga tgatattaat gctaagaaac ctgatttgat tattgtaggc
      181 ttaggcgctc ccaagcagga atttttccta tacaaacacc gatataatat acattccgga
      241 cttgcaatcg gagcgggcgg aaccattgac attatttccg gtttttccag aagggctccg
      301 gaattcttct gcaagcataa actggagtgg ttctacagac tgctaaaaca gcctgcaaga
      361 ttcaggagaa tgcttaacat tcctttgttc ctcataaagt gcaggcagca tgcaagaaag
      421 acaaaaaaag catacaagaa aaaaaacaga aggacttaag tctttctgct tttatgagga
      481 atatattatg aaattgttgt gttctcataa taaagaaaac atgtgaataa attatgacta
      541 tacggttaaa tctttgtgaa atttttatag cggtttcttt ttgggaactc cgtccggtct
      601 gggatattta tatgcggtat ttcttgtttt tttaaaatat atgttattcc ttttaatatc
      661 ggaaaaagga atggtctgtt tatcttcttt caatatcctg acagaaagtt gctttatggc
      721 gttttcggcc gattgcagtt cggaattgtc ggttttcatg gcaatgaaaa taccctctct
      781 ttttatgaaa ggtacacaat attccaaaag tatattcaac ggtgctacag cccttgccgc
      841 ggcaacatca aattgctctc tgtatacctc ttctcttgct gcattttccg ccctttcatt
      901 gcatacgaaa atattatcca aatccagagt ctttataaca tgctccagaa aagaaacttt
      961 tttgccgttt gcttcaagta acactatttt ttgtttgtat cccgctatct ttaagggtat
     1021 gccgggaaaa cctgcaccgc ttcccacatc aattaacgat tgggttgcct ctattccctc
     1081 atacagcaaa aggctgtctc cgaaatgctt tatataaaca tctcttaacc cggttattcc
     1141 ggtaagattg aactttttat tccattctat aagaagagaa gcgtattcat cgaatttttc
     1201 ataacactcc tcccggtaat ttattctcag ttcttcaaaa atacctttca gcagttgctt
     1261 cattctttag ttttcctttt ctttcgtttc tgctgctcaa tgtaaacata tagaacctgt
     1321 acatctgccg gcgatactcc cgaaatcctt cctgccatgc ccagtgaaac aggtttcacg
     1381 ctatccagtt tctgttgcgc ttcgtttctg agacctttaa tttttttgta ttctatatct
     1441 tcgggaagca gtaccttttc cattttttta aactgttcca cctgtctttg ttgtttttct
     1501 atataacctt cgtattttat ctttatctcg acctgctctt tttcttcttt tgtaagctcg
     1561 ggcatctcgg agtcaatttc atacagatcc tcatagccga taccttctct cttcagaata
     1621 gccgcttcgg ttgttccgga attaacaggt tgctgatttc ttttttccag aaaaggatta
     1681 attattttgg gtgaaatgtt cttctttttt aaccgatctg tttccttctc tatatttccc
     1741 atcttagaaa gaaaccgggt atatctttcc tttcctatca gtcccaattc gtaacctttc
     1801 ggtgtcagtc tttcatcggc attatcctgt ctcaacagaa ggcgatattc cgctctgctt
     1861 gtcatcattc tgtacggctc gttggttccc ttagttacaa gatcgtctat gagtactccg
     1921 atatatgcct ccgatctgtc caggacaaag ggttgtcgct ttttaatctt caagacagca
     1981 tttatacctg ccattattcc ctgtgccgcg gcttcttcat aaccgctgct tccgttactc
     2041 tgtccggcgg aataaagtcc cggaaatttt ttaaactcca atgtgggcaa aagctgcaac
     2101 ggatcaaagc agtcatattc aattgcatat gcacttcgca taaaaacaac attctccatg
     2161 ccttctatgg atttatacat ttcttcctgt acatcctccg gcatggaact gctcattccc
     2221 tgaacataca tctcgtcggt catcaaaccc ataggctcaa gaaagacctg atgccgctcc
     2281 ttatctgcaa atttaactat cttatcctca atagacggac aatatcgcac tcccgtaccg
     2341 cttatcttgc cgctgtacaa aggagatctg tccagatttc ttctgattat atcatgcgtt
     2401 ttttcattgg tatatgtaag gtaacagggt atctggtcta tttctatctt atccgtttca
     2461 aaagaaaaag gtacaatagg ctcatcgccg tcctgaacgg acatcttgct caaatcaata
     2521 ctgtttttat gcactcttgc aggtgttccc gttttgaatc gtcttaatat aatacccttc
     2581 ttttccagat taagggacag tttgtttgcg ggaaagaatc cgtcgggtcc gccttcataa
     2641 gacatatccc ctatgataat acgactcttc atataggttc cggttgcaat tattacggct
     2701 ttggcggaga tgataacatc ggtattggta accactccgg taactctgtt gtcgttgtca
     2761 aaaacaacat cggtaacctc atactgccgg atgtccagat tttcctgctt ttccagtata
     2821 tgtttcatca ccgtctgata ttgtcttctg tctatctgag ccctaagaga atagactgca
     2881 ggtcctccgg atttgttaag catttttgac tgtatgaggg ttgcatcggc acatttacct
     2941 atctgtccgc ccaaggcgtc tatttccctt accagatgtc cctttgctgt acctcctatg
     3001 ctgggattgc acggcatgtt ggcaatactg tccagattca aggtgaagat tgccgtcttt
     3061 aatcccattc ttgcaccggc cagtgctgct tcacatcccg catgtcccgc tccgacaaca
     3121 attacatcat attgcttcat attatttacc tatacagaat ttctcaaaaa ttctgtctat
     3181 aatatccgca cttgcggtct cccccgttat tttacccagg tcctccgcag cagctgttaa
     3241 atcaatactg agcatatcta taaaagaacc ctcttttgtg ttttcaagat ttgtaatggc
     3301 atccagtata tgttttttat gtctttcgtt tgttattgcg gagtctttgc tccttatact
     3361 tcctgcgtta accatatcca aaaatttgtc aaaaatctca ttgattcctt cctttttatc
     3421 caaagagcag gctataatcc tgtctttaaa taactttctc aattcttttt ccattgactc
     3481 gatatgcttt tccctgtctt tttcctgtat aagatcggtt ttattcagta cgcatactac
     3541 cttatccctg tctctcaagt aatggaatat ctcgtcttta tacggctggt ttatgtccgc
     3601 aatgtatata accgcatccg attgttctat cgcctgcttg gctttttcta tacctatctt
     3661 ctctatttca ttatccgtac tgcggatgcc tgcggtatct tttaatgtaa cacaaagtcc
     3721 ttttacatca atctttactt ccactatgtc ccttgttgtt ccggctatat cggtaacaat
     3781 tacacgattc tgcccacatg ctctgtttat gaatgtggat ttgcctacat ttacatcccc
     3841 tataaccgct acagtcaacc cttcccgtat aattcttctt tctttaaaat cctctacaaa
     3901 tacctttaat ctgtctgcta tattaagagc tttgtcctgc acctctttta cggttatctt
     3961 ttccgtatca tattccggat attccataac caccattatg tgtgaaatga ggtcgataag
     4021 ttcacttcta atactcttaa tctgtaaact tttttctcct tttatctgtg acaacgcctc
     4081 tttgtaagca tcttccgctt ggctgtttat aaggtccatg accgcttccg cttccataag
     4141 gtcaatgctg ccgtttataa atgctctttt tgtaaactcg cccggttgtg cgggagcagc
     4201 tccgaatgca tatgccaaag caagtatttt tcttgtcacg tataatccgc cgtggcagtg
     4261 aatttccacg gtgtcttctt tggtataggt tttcggagct ttcatcttaa gaagcagtac
     4321 attatccaat gtattgccct ttttgtcttg aatatgtccg taagttaccg tgtggctttt
     4381 gatatcttta aaaggagttt tgctcttaaa catactttca gcaaccgaaa aggcattgct
     4441 tccgctaatt cttatcaccg atattcctcc aacccctaca ggtgtggaaa ttgctgctat
     4501 agtatgttcc atttttaata gttgtttctt cttccgtctc tttcaaagga aattactatt
     4561 tttctgtatg gctcgtctcc gatactgtat gtggaaatat tctccctgtt ctgaagacat
     4621 gtgtgtatta tccttctgtc atatgaagac atgggatcca gcataaaatc ccttttggct
     4681 ttgattactt tatccgcagt ctttcttgca agctcttcaa ggtaagcctt gtgtcgttcc
     4741 ctgtaatttt ccgtatctat tataaccctt atatacgtgt cataagctct gcttactacc
     4801 tgagatgtta taaactgtaa tgcctggagt acttctcctt ttcttccgat aataagtccg
     4861 gaatcgctac agctaatttc cgccttgtaa aagccgtctt cttcgctgac aaatacatcg
     4921 ggcgcaagat tcatctgtga aagaacatcc tctaagaaaa acttaattgt ttctttggga
     4981 tcatcttccg tggaaaaata cactcttacc tttgcttctt tccttcccag cccgagaagt
     5041 cccttggttt cctcctcaac tatttctatt tccacatctc cgagttccag gcctaattcc
     5101 gaaagcgccg cctgtaccgc ttcatcaacc gtttttcctg tcttttcaat gcttaccctg
     5161 ctcatttttt atcgttactc tccttgcttt cctctttaac ttcatatgtt gcttcaataa
     5221 cttcctcacc ctgtgatgca gcgccgcttc ttacagcttc ggcttctttc tttgcatcgt
     5281 ggtttttcct tattctttcc attatgaaat gctgaactat ctggacaata tgtgtgaata
     5341 tccagtacag cgacatgctg acaggtaatg taaatcctat ccataatgtc atagcgggca
     5401 ttaaaaactt cattgtttta cccatctgct ctgacatatc cggagcatcg ttgggattgg
     5461 gagccttttt ctttgccggc attctttttt tcatagcctt atcggtcaga tattgtgaaa
     5521 gtataaccaa tcccaccgat attaaaggca gaaacagaat gggaagatat gtccctatcg
     5581 tttccgcacc gaaaagtatg gacggattaa ccttcggaac caatccgaga ttcattccta
     5641 agaaattcat atcaataagt ttgtcaaaag atatgagatc ctttacaagt ttgaatttag
     5701 caggatcgga aataaggtcg ttgtttatgg tagtctgtaa aattgtcggg gcatacccta
     5761 atctctctat tatcagatta atcgtgtctt tggaaactcc ctttatgtaa gtaagaggtt
     5821 gtgcaagaac tctgtacatt gctatcagca acggaaactg aatcagggta ggcagacaac
     5881 ctgatgcaca acctacattt tcctgtttat acagtttctg aatttcttca ttctgtttct
     5941 gctgatcgtt tttatacctt ctctttatgg catccacttt cggtgtaagc tcggtcattc
     6001 tcattgtgga tttctgacct ttgatagaaa gcggcatcaa aagaagcttt aagagcagag
     6061 tgtatacgat tatggttatt ccgaaattct gtccgcaaag accggtatag agtaattcaa
     6121 atatctttcc gaacaagttg taaataaaat caaacatagc ccttcctttc tctatttaac
     6181 aggatcataa ccgcccttaa aaagcggatt acatctcaaa attctccaca gcgataacaa
     6241 actaccctta aaagcaccat gtttttccac tgcttcaata gcatactgag agcaggtggg
     6301 aacaaatctg caacacggta acttgttggg ggagatattg attctgtaaa atttaatcaa
     6361 tctgattatc agttttttca ttctttatta tcaattttgc cttatccagc aaatcgacta
     6421 tttccttctc tatttcatgg tattcaggta tttcatcagt tttcttaact acgaatacca
     6481 tatcataccc gtttttcaga ttaaagttac ctgtccttaa tatttccttt attcttctac
     6541 gatataaatt tcttttaacg ctacttttgt atgctttgct gcttactgat atgcctaccc
     6601 tgttgcgcaa acatgcattt tccagaacgt ttattgtcaa atactttccg ctgaaatatt
     6661 ttccttttct gtatacacgt aaaaactcat agttcttttt cagcgtttcc atatttaacc
     6721 tttcgtataa ataaaggcat gagaaaggaa tcctatgcag ataatctctt cctgcccctg
     6781 gctcttctgt tcttaataat ctttcttccg gcagccgtct tcattctctg tctgaatccg
     6841 tggactctgc ttctttgttt agttttcggc tgatatgttc ttttcatcag taactcctat
     6901 atataaaaaa atttatctcc tctttttgtg tacatgcgta cacagcgtat tagattatat
     6961 atatttacaa tataaaatgt caaggtcttt taatctgcgg cacaatgcat tatgctattc
     7021 tcttttttct ctactatata tagtattttc aaattcataa gaccacaatg attaccgata
     7081 ttccttaaat agtagtgtta aatatccggt atgcaggtat agagtgaaat aattacatat
     7141 atgttaggcg catataacat ttgccgcttt gaggttttct taaagatgct ttatggtttg
     7201 ttgctcttta agcgcaaaaa gtccgcaaac ccgaacgaat ctgcggacaa attgtggcgg
     7261 agaagatggg attcgaaccc atgcgccggt tgcccgacct aacggttt
//
LOCUS       DAOPWC010000021         7181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_10420_length_7181_cov_3.440359, whole genome shotgun sequence.
ACCESSION   DAOPWC010000021 DAOPWC010000000
VERSION     DAOPWC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10420_length_7181_cov_3.440359"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKJ65_00925"
     CDS             complement(<1..332)
                     /locus_tag="PKJ65_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40430.1"
                     /translation="MTETKYTLRDAFIEAKRIKLSGIPKDEDIDWTPSPRFERKINRL
                     IKNSRKRYWPLINTPLKKVAVAAMVIIMLFGFTMSIEAAREAVVDYIITVYEKYTGIF
                     FDVDDKDL"
     gene            complement(325..888)
                     /locus_tag="PKJ65_00930"
     CDS             complement(325..888)
                     /locus_tag="PKJ65_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ40431.1"
                     /translation="MIVFLSLTDNDEDKIKLENMYNRYSKRMFYVAMDILKNEYDAED
                     AVHNTFISIAVNMDKINDIRSEETYYYVMTITENTALNLLKKKKGESRKTINLDFEDD
                     SWLFVSDIDIAEEFVSKEEYNRIVDIIQSLPDIYSDVLYLHYVENLTAREIASALSRK
                     QETVRKQIVRGKKMLIHYLRKEGMEND"
     gene            complement(1088..1954)
                     /locus_tag="PKJ65_00935"
     CDS             complement(1088..1954)
                     /locus_tag="PKJ65_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNZ40432.1"
                     /translation="MKTQERKKIISVKNVSFTYAKNTPFEKKALEDINLDFYGNEYTA
                     IIGATGSGKSTLIQHLNGLLKPDKGEVTVDGIDTKDKKAQGDLRRKVGLVFQYAQYQL
                     FEETVYKDIEFGLIKRNVPEEERKKRVPEAAKLAGLTEENLKVSPYDLSGGQKKRAAI
                     AGILVLKPEILVLDEPAAGLDPEGTRELFATIQNLHKSKECTVILVAHSMELVALYAE
                     RIIVLDQGKVVMDGTRDEVFGNEEKLLSLGLDIPEITKVMKKIKEKHPNINDRIYTVD
                     DAKEEILKNLAK"
     gene            complement(1954..2781)
                     /locus_tag="PKJ65_00940"
     CDS             complement(1954..2781)
                     /locus_tag="PKJ65_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNZ40433.1"
                     /translation="MIEVKNAHYSYKTQPETTKAVSGISLSIAKGRYIAILGRNGSGK
                     STLARMLNALIIPDSGDIIVLGMNTKEKENQLPIRKKVGMIFQDPDNQIVGTRVLEDV
                     AFGPENLGYERQKIIQRVDDALHQIGIYDLRERAVHTLSGGQKQRVAIAGVLAMRPAC
                     IILDESTSMLDPKGQAELLSVVDKLNKEEGISIINITHNMDEARFADEVYVLHQGTLT
                     LKGSPEEVFTDVDNMLKAGLDVPQATKLLYELKKEKINIEDLTIDRNRAADIIGRLL"
     gene            complement(2824..3330)
                     /gene="rplQ"
                     /locus_tag="PKJ65_00945"
     CDS             complement(2824..3330)
                     /gene="rplQ"
                     /locus_tag="PKJ65_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNZ40434.1"
                     /translation="MPSHRKLDLPTDQRIAVLKNLVTGLIMTGKIETTEARGKEVKKI
                     ADSIIAKAVKECDNFTSKPQKVTRPKTHKGRRLTMTKESKYKVDYEVIERETVEEMVT
                     VDSPSRLHARRQIFKWVNKVDGVNVVNKLFDEIAPRLKDRQGGYVKIYKLGKRRGDAA
                     EMVLLEII"
     gene            complement(3342..4280)
                     /locus_tag="PKJ65_00950"
     CDS             complement(3342..4280)
                     /locus_tag="PKJ65_00950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNZ40435.1"
                     /translation="MTIEKPRIECVETTDVYGKFVVEPLERGYGTTLGNSLRRILLSS
                     LPGAAATSIKIDGVLHEFSTIPGVVEDVTEIILNVKGIRFRLDTDEVKTLSIRTEGTG
                     DVTAGDILKNAEVEVLNPDHYLFKMDECKSFYMEITVSKGRGYDSAEKNKKEGMPIGT
                     IPIDSIYTPVNRVVYKVEDTRVGQITDYDMLTMEIYTDKSMSPTKALSYAAKIMSDHL
                     NLFIDLSEEVKQTETVKEAEETNKDESLEKPIDDLDLSVRSYNCLKRAGINTVGNLIS
                     KTEEEMMKVRNLGKKSFEEIHNKLDELGYKFAKSDE"
     gene            complement(4332..>4733)
                     /gene="rpsD"
                     /locus_tag="PKJ65_00955"
     CDS             complement(4332..>4733)
                     /gene="rpsD"
                     /locus_tag="PKJ65_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ40436.1"
                     /translation="KYFEMASRQKGMTGENLLKILESRLDNIIYRIGLANSRPEARQL
                     VKHGHFTINGKTVDIPSYLVQVGEVIQLKESMKDSPKFKELIETSQTKIIPAHIEFDQ
                     SAVSAKIVDTCKREDVSLPVEERFIVEFYSK"
     assembly_gap    4734..4743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4744..4969)
                     /locus_tag="PKJ65_00960"
     CDS             complement(<4744..4969)
                     /locus_tag="PKJ65_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ40437.1"
                     /translation="MARYTDAVCKLCRREGTKLFLKGDRCYTDKCALVRRAYASGQHG
                     KKRSKQSEYGMQLREKQKARRFYGVLESQFR"
     gene            complement(4985..5383)
                     /gene="rpsK"
                     /locus_tag="PKJ65_00965"
     CDS             complement(4985..5383)
                     /gene="rpsK"
                     /locus_tag="PKJ65_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNZ40438.1"
                     /translation="MAQKTTVKRSRKRKEKKNVEHGQAHIRSSFNNTIVTMSDLQGNV
                     LSWASAGGLGFRGSKKNTPFAAQVAAETAAKAAMEHGLRTVEVYIKGPGAGRESAIRA
                     METAGLTVVMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(5397..5768)
                     /gene="rpsM"
                     /locus_tag="PKJ65_00970"
     CDS             complement(5397..5768)
                     /gene="rpsM"
                     /locus_tag="PKJ65_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ40439.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIYGIGLTSSQKILEQAGVNPDTR
                     VKDLTESEIVKLRGLIEKEHVVEGDLRREVSFNIKRLVDIGSYRGRRHRMGLPVRGQR
                     TKTNARTRKGPVRASVSVKKK"
     gene            complement(5876..5989)
                     /gene="rpmJ"
                     /locus_tag="PKJ65_00975"
     CDS             complement(5876..5989)
                     /gene="rpmJ"
                     /locus_tag="PKJ65_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNZ40440.1"
                     /translation="MKVKPSVKKRCEKCKIIKRKGRIMVICENPRHKQRQG"
     gene            complement(6002..6217)
                     /gene="infA"
                     /locus_tag="PKJ65_00980"
     CDS             complement(6002..6217)
                     /gene="infA"
                     /locus_tag="PKJ65_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ40441.1"
                     /translation="MANDVIETEGIVIESLPNTMFRVELPNKHVILAHISGKLRMHYI
                     RILPGDKVKIEMSPYDLTKGRIVWRGK"
     gene            complement(6236..6907)
                     /locus_tag="PKJ65_00985"
     CDS             complement(6236..6907)
                     /locus_tag="PKJ65_00985"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNZ40442.1"
                     /translation="MIIIMLGAPASGKGTQSVVVAQKLGVPHISTGDIFRWNIANNTP
                     IGEKAKKYIDKGMLVPDELTIQILKERIEMDDCKDGFVLDGFPRTINQQESLDKMLAD
                     CQKSIDMAINLVVTDNEVIRRISGRRMCKCGKAYHIVSYPSKTPGICDLCNGELYTRK
                     DDTIEVAKTRLDTYKKQSEPIIAVYREKGILIDIDGHKPQDEVTEDVLNEIEKYSSKQ
                     VWYNA"
     gene            complement(6915..>7181)
                     /locus_tag="PKJ65_00990"
     CDS             complement(6915..>7181)
                     /locus_tag="PKJ65_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ40443.1"
                     /translation="KKNGGFIPGLRPGKPTAEYLAYVLNKITWFGGLFLALLATIPSV
                     LARIVGIQIGFGGSALLIVVGVALETVKQMESQMLMRHYKGFLD"
BASE COUNT         1673 a         1689 c         1184 g         2625 t
ORIGIN      
        1 ttaaggtctt tatcatcaac atcaaagaag atacctgtgt atttttcata tactgtaata
       61 atataatcga ccacagcctc tctggctgct tctatgctca tagtaaaacc gaacagcatt
      121 attattacca ttgcggcaac agcaactttt tttaacgggg tattaattaa aggccaatat
      181 cttttcctgc tgtttttaat cagtctgttt atctttcttt caaacctcgg agacggagtc
      241 cagtctatat cctcatcttt aggtattccg gacaacttta ttcttttagc ctctataaat
      301 gcatcacgca atgtgtattt tgtttcagtc attttccata ccttcttttc tcagatagtg
      361 tataagcatt ttcttgcctc ttactatctg ctttctaact gtttcctgct ttcttgataa
      421 tgccgatgct atttcccggg cagtaaggtt ttccacataa tgcaagtaaa gcacatcgct
      481 gtaaatatcg ggtagacttt gtatgatatc gacgatacgg ttatattctt ctttagatac
      541 gaattcctcc gcaatatcaa tatcactgac aaacaaccag gaatcatctt caaaatcaag
      601 gtttattgtt tttctgctct cccctttctt cttttttaat aagttaaggg cagtattttc
      661 cgtaatcgtc atgacataat agtatgtttc ttcggaacga atatcgttaa tcttatccat
      721 attgacagca atggaaataa acgtattatg cactgcatct tcagcatcat attcattttt
      781 cagtatatcc atagcaacat aaaacattct tttgctataa cgattataca tattttctaa
      841 ttttatttta tcctcatcat tatctgtcag gcttagaaat actatcatta atcctccgtg
      901 atatacggta tatgttgtgc aaaaactaca caagcatatt agcacatatc cgtctcgggt
      961 gcaataaatg cattcctgta ttaaacatgc atctttgata agcattcctt cttacctctc
     1021 tatatatata acaatcctga aaggcaaaac ggggacaaaa attctgaact ttttttaaat
     1081 tttcttatta ttttgccaaa ttcttaagaa tttcctcttt tgcatcgtct accgtgtata
     1141 tacggtcgtt tatattcggg tgcttttcct ttattttctt catcaccttt gttatttccg
     1201 gtatgtctaa acctaaagac aggagtttct cttcattacc gaatacttca tctcttgtgc
     1261 cgtccataac tactttacct tggtccagta caattattct ttcggcatat agtgcaacaa
     1321 gttccatgct gtgagctacc agtataacgg tgcactcttt gcttttgtgc agattctgta
     1381 tggtggcaaa caactctctt gttccttcgg gatccagtcc cgctgccggt tcgtcaagaa
     1441 ccagtatctc gggttttaat acaagaattc ccgcaatggc cgcacgcttt ttctgtcctc
     1501 ccgaaaggtc gtacggagaa actttaaggt tttcttctgt taagcccgca agttttgcgg
     1561 cttctggcac tcttttcttt ctttcttctt ccggaacatt tctttttatc agtccgaact
     1621 ctatatcctt gtaaacggtc tcttcaaaca gctgatattg tgcatactgg aataccagac
     1681 cgacttttct tcttaagtct ccctgagctt tcttatcctt tgtatctatt ccgtctaccg
     1741 taacctcacc tttatcgggt tttaaaaggc cgttcagatg ttgtataagg gtggatttac
     1801 cgctgccggt agctcctatt atggctgtat attcattgcc gtagaagtct aaattaatat
     1861 cctccaaagc cttcttctca aaaggagtgt tctttgcata ggtaaaagaa acattcttta
     1921 cggatattat cttttttctt tcttgggttt tcattatagc aaccttccta ttatatccgc
     1981 agctcggttt cggtctattg taagatcttc tatgtttatc ttttcttttt tcaactcata
     2041 caacagtttg gtcgcctgcg gtacatccaa tcccgctttg agcatattat ccacatcggt
     2101 gaatacctct tcaggagatc cttttaatgt taaggttcct tgatgcagca catacacttc
     2161 atctgcaaac ctggcttcat ccatgttatg agtgatattt attatactta ttccttcttc
     2221 tttattgagt ttatccacaa cacttaacag ttcggcctgt cctttgggat ccaacatgga
     2281 agtggattca tcaagtatta tacatgccgg tctcatggcc agcactcccg ctatggcaac
     2341 cctctgtttc tgtccgcccg acaaggtatg aaccgctctt tctcttaagt catatatacc
     2401 tatctgatgc agggcatcat ctacccgttg tataatcttc tgtctttcat atcctaagtt
     2461 ttccgggccg aatgcaacat cttccagcac tcttgtgccc actatctggt tgtccgggtc
     2521 ctgaaatatc atgcctacct tttttcttat gggcagttga ttttcttttt ctttggtatt
     2581 catacccagt acgattatat ctccgctatc gggaatgata agtgcattaa gcattctggc
     2641 aagtgtggac ttgccggaac cgtttcttcc taaaattgct atgtatcttc ctttagctat
     2701 agacaaggaa ataccggaaa cggcctttgt tgtttccggt tgtgtcttgt atgagtaatg
     2761 tgcattcttt acttcaatca tccttaatat taaacaagcc ttccttgaag gcttattttc
     2821 tacttaaatt atctcaagta acaccatctc tgcggcatca ccgcgtctct ttcccaactt
     2881 atatatcttg acatatccgc cctgtctgtc tttgagcctg ggtgcaattt catcaaaaag
     2941 cttgttgacc acgttaactc cgtctacttt gttaacccat ttgaatatct gtcttctggc
     3001 atgtagtctt gagggactgt ccacggtaac catttcttct accgtttctc tctcaataac
     3061 ttcgtaatcc accttgtatt tgctttcctt ggtcatggta agccttctgc ctttatgtgt
     3121 cttgggacga gtgactttct gaggcttgga tgtaaaatta tcgcactctt taacagcttt
     3181 agctattatg ctgtcggcta tcttctttac ttcctttcct cttgcttcgg ttgtttcaat
     3241 ttttcctgtc attatcaaac ccgtaaccag attcttaaga actgctattc tctggtctgt
     3301 aggtaagtca agttttctat gtgacggcat tttattcctc cttattcatc cgacttggca
     3361 aatttatatc ccaattcgtc aagcttgtta tgtatttcct caaatgattt cttgccgaga
     3421 ttgcgaacct tcatcatttc ctcctcggtc ttgctgatta ggttgcctac ggtgttgatt
     3481 ccggctctct ttaagcagtt gtatgatctt accgaaaggt ccaaatcatc aatgggtttt
     3541 tccaagcttt cgtccttgtt ggtttcttcg gcttctttaa cggtttccgt ctgtttgact
     3601 tcttccgaaa gatcgataaa cagattgaga tgatcgctca ttatcttagc cgcataactc
     3661 aacgcctttg tcggggacat tgatttgtcc gtatatattt ccattgtgag catgtcatag
     3721 tcggttatct gacccacacg ggtatcctcc actttatata ccactctgtt taccggggta
     3781 tatatcgagt cgatgggaat ggttccgata ggcatacctt cttttttgtt cttttcggcg
     3841 gaatcgtatc ctctgccttt ggataccgtt atttccatgt agaaagattt acattcatcc
     3901 atcttaaaaa gataatgatc gggattgagt acttccactt cggcattctt aagaatatct
     3961 cccgcggtaa catctccggt accttcggtt cttatgctta aagtctttac ttcatcggta
     4021 tccagtctga acctgatacc tttaacatta agaattatct cggtaacatc ttccactact
     4081 cccgggattg tggaaaattc atgcaatacg ccgtctatct ttatgctcgt agcagctgct
     4141 ccgggcaaag aggataacaa aattcttctt aaggagttac ccaatgtagt gccgtagcct
     4201 ctctcgaggg gttccactac aaacttgccg taaacatcgg ttgtttcaac acattcgatt
     4261 ctcggtttct ctattgttat atctatcaat gttataccct ccttttaaac taatggtata
     4321 ttaagcagtt actacttgga gtagaattcc actataaatc tttcctctac aggcagactt
     4381 acgtcttcac gtttacatgt atcaacaatc tttgcgctca cggcggactg atcgaattct
     4441 atgtgtgccg gtattatctt ggtctgtgag gtttcgataa gttccttgaa cttgggcgag
     4501 tctttcatgg actccttgag ttgtatgact tctcccacct gtaccagata tgacggtata
     4561 tccactgtct taccgtttat tgtaaagtgc ccgtgtttga caagctgtct tgcttccggt
     4621 ctggaattag ccagtcctat cctgtaaata atattgtcca gtctgctttc caatatctta
     4681 agaaggtttt caccggtcat gcctttctgt ctggaagcca tttcaaaata cttnnnnnnn
     4741 nnnttctgaa ctgtgattcc agtactccgt aaaatcttct tgctttctgc ttttcacgaa
     4801 gctgcatacc gtattcgctc tgtttgcttc tcttctttcc gtgctgtccg gaagcataag
     4861 ctcttcttac caaagcacat ttatcggtat agcatctgtc accttttaaa aacaattttg
     4921 ttccttcgcg tctgcaaagt ttgcatacag catctgtata tcttgccatg ttaattgtac
     4981 ctccttaaac tcttcttctc ttcggcggtc tgcagccgtt atgcggaata ggagttacat
     5041 cctttatcat tacaacagta aggcctgcgg tttccatagc cctgattgcc gattctctgc
     5101 cggcaccggg tccttttata tatacttcca ccgttctcaa gccgtgctcc atagcagctt
     5161 tagcagctgt ctctgcggca acctgtgcgg caaacggtgt gtttttcttg gagcctctga
     5221 aacctaatcc gcccgcgctt gcccatgata aaacattacc ttgtaaatcc gacatggtta
     5281 caattgtatt attgaagctg gaacggatgt gtgcctggcc gtgttcaacg tttttctttt
     5341 cttttctctt tcttgatctc ttaacagtcg ttttctgcgc cattgctcat gtctccttac
     5401 ttcttcttta cactaacaga tgctctgacc ggacctttcc gtgtccttgc attggttttt
     5461 gttctctgac cccttacagg taagcccatt ctgtgtcttc tgcctctgta gcttcctata
     5521 tcaaccagtc tttttatgtt gaatgatact tctctgcgaa gatcaccttc aactacatgt
     5581 tctttttcta tcaggcctct caattttaca atttcagact ccgtaagatc ttttactctg
     5641 gtgtcaggat ttactcctgc ctgctccaga attttctgtg aactggtcag tccgataccg
     5701 tatatgtatg tcagaccgat ctctactctt ttctctctgg gtaaatccac cccggcaata
     5761 cgagccattt tactttttta cctccatagt cctttcaaaa tctttcgagt atctatgtta
     5821 tcactgctaa gaacagtatt tataccgtcc agccgcttta gcagcgctaa tcacattatc
     5881 cctgtctttg tttatgtctg ggattttcgc agataaccat tattctgcct tttcttttaa
     5941 ttatcttgca cttttcacat ctttttttaa ctgatggttt aactttcatt atatattcct
     6001 cctatttgcc tctccatact atccgacctt tggtcagatc atatggtgac atctcaattt
     6061 ttaccttatc tcccggtaat atccttatat agtgcattct cagttttcct gagatgtgtg
     6121 ccaatattac atgcttgttg ggtaactcga cccgaaacat tgtgtttggt aaagattcta
     6181 ttactatgcc ttctgtctca attacatcgt ttgccaaaac ttttgcctcc ttatcctaag
     6241 cattatacca cacttgtttt gaagagtatt tttctatttc atttaacaca tcttctgtaa
     6301 cttcgtcctg gggtttatgc ccgtctatat caataagaat ccctttttct ctgtacaccg
     6361 caataatcgg ttctgactgt ttcttatatg tatccagtct tgtttttgcg acttctatgg
     6421 tgtcatcttt tctagtgtaa agctcgccat tacatagatc acatattcct ggcgtctttg
     6481 aaggatatga cacaatgtga taagcttttc cacatttaca catccttctg cctgatatcc
     6541 ttcttatgac ttcattgtct gttacaacaa gattaatagc catatcgatg gatttttggc
     6601 aatcagctag cattttatcc aaagattcct gctgattgat tgtcctggga aaaccgtcaa
     6661 gtacgaatcc atccttgcag tcatccattt caattctttc cttcaatatc tgtattgtca
     6721 gctcatccgg aaccaacata cctttatcaa tgtacttctt tgccttttct cctattggag
     6781 tattgttggc tatattccac ctgaatatat cacctgttga tatatgtggt actcccagtt
     6841 tttgtgctac tacaacgctt tgcgtgccct tgccgctagc aggagctccc aacataatga
     6901 taatcatggt tctcctaatc aaggaatccc ttgtaatgcc tcatgagcat ctgtgattcc
     6961 atttgtttaa ctgtttctag tgcgacacct acgacaatca gtaaagcgct tcctccaaac
     7021 cctatctgta tgccaactat tcttgcgaga actgatggta ttgttgctaa tagggcaagg
     7081 aaaagacctc caaaccacgt tatcttattc aatacatatg ctagatactc tgctgtaggt
     7141 ttaccaggcc ttaaaccagg aataaatcca ccattcttct t
//
LOCUS       DAOPWC010000022         7114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_10545_length_7114_cov_4.166029, whole genome shotgun sequence.
ACCESSION   DAOPWC010000022 DAOPWC010000000
VERSION     DAOPWC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_7114_cov_4.166029"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKJ65_00995"
     CDS             <1..261
                     /locus_tag="PKJ65_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40444.1"
                     /translation="TQLQAKKAVEAGYWHLYRYNPKLAEEGKNPFILDSKEPTGSFKD
                     FLATETRYTSLKVTFPDHAEELFEKTEQDAKERYETYRKKAE"
     gene            444..1475
                     /locus_tag="PKJ65_01000"
     CDS             444..1475
                     /locus_tag="PKJ65_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNZ40445.1"
                     /translation="MFDEATRKILHACPREALDESLFSTSPIITRYPDTILSRHDVPY
                     SAALTFNAGVVKTDDRYIMIFRNDYGSFEEEKIIGTNLGLAFSDDGIKWKVEPEPCFA
                     MADDEVLRVYDPRITVLEDRYIICFAVDTKHGVRGGIAETKDLKHFEVLSMSVPENRN
                     MVLFPEKINGNYVRLERPMPVYGRDGMKFDIFLSESPDLVYWGKSRLVMGVEHVPFAN
                     EKIGPGAPPIKTDKGWIMLFHAVDFDPARGKNGWEPTWKKRYTIGVALLDLKDPSKVI
                     GMYKRPLMIPEGYYELEQGYRTNALFPCAALVDEGNVVRIYYSAGDAIIRMATCDLDE
                     LIGLCTEPL"
     gene            1477..2436
                     /locus_tag="PKJ65_01005"
     CDS             1477..2436
                     /locus_tag="PKJ65_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ40446.1"
                     /translation="MIQTNYINSPIVITPFKKRVITYAFHDIDGTHSLIRDWPPVMSI
                     VLNDVIENGLKEGYDSERNVKRLVKNAGTKPLPETDRFCVESAGLSALTQMEWAIRRG
                     VQEKTVTVQCDDSVNSEIISLIWQGKEEWHHIAETEEMKQMLTENTPRLFKYYEKVLN
                     GFCRDKNLVLARKDPEKFRIKGSFRFLNYLKENGIKNYFVTGAVVQRGMGMFEEVETL
                     GYKIGHGEIVEDIIGSTWTDKVPKNVIMNQLLDKYGIKGENTMVVGDGRSEIQAGVDM
                     GAFTMSRLCTHMQYQKQLHIRMGTNVIVEDFEHEDIYKILIAK"
     gene            2631..3725
                     /locus_tag="PKJ65_01010"
     CDS             2631..3725
                     /locus_tag="PKJ65_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40447.1"
                     /translation="MDFYPVDYLKENISDSGCIRECIEQAVKINGHKRIIFNKRNWHI
                     DEAILLHSDMEIIVDGCFIKQNDGVFDNIFRGVNLRVDEKDPYGKPLYVEKMKNVSIK
                     GYNNAKLIGCDVNKKGYHPVKKEYEDMVGDNWGWRTFMICLSECEGIEISGLSIENTK
                     CWALSLDLCSYGDIHDIFIQSDVKNGDGIDLRAGCRHFRIWNITGNTSDDTVACTALL
                     HSKQRFPFKNYLYPLAPAVSVFDGDIRKLDIHDISVSDIRTGGLHHAVICLAADGLRV
                     YNIDIKNIEDIGEGNRSATVMLYTGYGENYADNDIHDINIYGVHSRFSKYAVHINTKV
                     HNVTVSDILQENKEGSLYEFADTEGITVLN"
     gene            complement(3722..5053)
                     /locus_tag="PKJ65_01015"
     CDS             complement(3722..5053)
                     /locus_tag="PKJ65_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40448.1"
                     /translation="MLRRKVENDLLKWKRANNKTALLVTGARQVGKTASIRQFGKTYY
                     DNFIEINFITDINADNIFSGSLRADDIILNLSAFVKKALVPGKTLIFLDEIQECPNAR
                     TAIKFLVEDGRFDYIESGSLLGINYKEIPSLPVGYEEILQMYPMDFEEFLWANNVQED
                     TIDYLKDCFINEKCISEVVHEEIKKLFQRYMIIGGMPAVVSKFAETKDIGQVIAVQKR
                     IMELYRQDITKYSETEKMRIRQIFDHIPAELNKNNKRFTLSDMDKNARYNRYESGFMW
                     LSDAGVSLPCYNISQPVLPFELNTKYNLFKLFLCDTGLLCSQSLGNIQFAVLNGDTSV
                     NMGSILENTIAQLLVSNNFKLYYYNSKKVGEIDFIIQKGAIALPVECKSGKDYNLHKA
                     LDNLLSVKEYDMKEAIVLYSGNIKRENKILYLPWYMVSFITQPSIDSYIVN"
     misc_binding    5242..5454
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5499..>7114
                     /locus_tag="PKJ65_01020"
     CDS             5499..>7114
                     /locus_tag="PKJ65_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ40449.1"
                     /translation="MKEEIKRPVFPKRAVITSGMPYGNKGLHFGHIGGLFVHADVFAR
                     FMRDRIGEENVIFISGTDCYGSPILESYRKLKESGNTEHKNIVEYVESNHIKQKNTLD
                     AYEISLNHFGASGLYRDKEIHRKVSIEVFESLYKGGYLSVMSTPQFYDPKFKTLLNGR
                     QVEGKCPFEGCGSEKAYADECGLGHQYMPEELIDPKSTLSGLKPEIVDVENWYFRLED
                     FRDKMQEYTKDLREHSVTRKFVLDAIDEFLKPPVIYVQRKQIDDLEDLERAFPEHETI
                     DEENKASITFVFKNLDDRDIARDVLNERQIRYRTGKTLVPFRLSGNIDWGVPIPEKDN
                     LKDLTFWVWPESLWAPISFLRTYLEEKGAKEDEWKKWWLDDESNVYQFIGEDNIYFYC
                     IAEMAMFAAMKHPDFDTLDMTKLHLPDIIANKHILFMDKKASSSSQIKPPMANDLLEY
                     YTAEQLRMHFISLGLTSKSSSFKPQALLPKEEREGVDPALKEGNLLTNVFNRLIRSCL
                     YSIQKYNDSIVTTGKVSEEIKQESDDAILE"
BASE COUNT         2270 a         1243 c         1622 g         1979 t
ORIGIN      
        1 actcagctac aggcaaagaa agccgtggaa gcgggatact ggcatcttta cagatacaat
       61 ccgaaacttg ccgaggaagg aaagaatccg ttcattctgg actccaagga acccacggga
      121 agcttcaagg atttcctggc taccgaaaca agatatactt ctctgaaggt cacattcccc
      181 gaccatgcgg aagagctgtt tgaaaagacc gaacaggatg ccaaagaaag atacgagacc
      241 tacagaaaga aagccgaata agtaaaggta atattcgaat aaatcaagat gactgtacaa
      301 aatgtgcagt cattttgtat gtgaagggga gtttgccgat acttcaataa ccggaaaaca
      361 cgctgcttga tgttgactta gcagccggat ttgtatatat taagagttgc tataacaata
      421 tataaaaagg aaggtatttt caaatgtttg atgaagcaac ccgcaagata ttacatgctt
      481 gtccgagaga agcgcttgat gagagtctgt tttccacaag ccccataata acaagatatc
      541 ccgatacgat tctgagcaga catgatgttc cttattccgc ggcattgacc tttaatgcag
      601 gagttgtaaa gaccgatgac agatacatca tgattttccg taatgattac ggttcattcg
      661 aagaagagaa gattatcgga accaatctgg gactggcttt cagtgatgac ggaattaagt
      721 ggaaggtgga gccggaaccg tgttttgcca tggctgacga tgaagtgctc agagtttatg
      781 acccgagaat aaccgtcttg gaggacagat atataatctg ttttgcagtg gataccaagc
      841 acggagttcg cggaggaata gccgaaacaa aagacctcaa acattttgaa gtactgtcga
      901 tgtcggtacc ggaaaacaga aatatggttc tgttccccga gaaaataaac ggtaactatg
      961 ttcgtttaga aagacccatg ccggtttacg gaagagacgg aatgaaattc gatattttcc
     1021 tttcggaatc acctgacctt gtttactggg gtaaatccag gctggtaatg ggtgtagagc
     1081 atgtcccgtt cgcaaatgag aagataggac cgggtgcacc tcctatcaaa acggacaaag
     1141 gctggataat gttgttccac gcagtggatt ttgatcccgc aagagggaaa aacggatggg
     1201 agcctacatg gaaaaagaga tatacaatag gtgttgcttt actggatttg aaagatccgt
     1261 caaaagtaat aggaatgtat aaacgcccgt taatgatacc tgaaggatat tatgagctgg
     1321 aacagggata cagaaccaat gcattgttcc cgtgtgccgc tcttgttgat gaaggtaatg
     1381 tggtaaggat atattacagt gcgggagatg ccataatccg tatggcaacg tgcgatcttg
     1441 atgagcttat aggcttgtgt accgaaccgt tataatatga tacagacaaa ttatattaat
     1501 tcacccatag taatcacccc tttcaaaaaa agggtgatta cttatgcctt tcatgatatt
     1561 gacggcacac attcattaat acgagactgg ccacccgtaa tgagcatagt tttaaacgat
     1621 gtaatagaaa acggattaaa agaaggctat gacagcgaaa ggaatgtaaa aagactggtg
     1681 aaaaatgccg gaaccaaacc cttaccggag accgacaggt tctgtgtgga atcggcagga
     1741 ctttccgctt tgactcagat ggaatgggcg ataaggaggg gtgttcagga gaaaaccgtc
     1801 acagtgcagt gtgatgattc ggtaaacagc gagataatca gccttatatg gcagggaaaa
     1861 gaagaatggc accatatagc ggaaaccgaa gagatgaaac agatgcttac ggagaacacc
     1921 cccagattgt ttaaatacta tgaaaaagta ttgaacggtt tctgtcggga taaaaacctc
     1981 gtacttgcaa gaaaagaccc ggagaaattc cgaataaagg gttctttcag atttttgaac
     2041 tatcttaaag aaaacggaat taagaactat ttcgttaccg gagcggtggt gcaaagagga
     2101 atgggcatgt ttgaggaagt ggagacgctg gggtataaga taggtcacgg agagattgtg
     2161 gaagatataa taggctccac atggacggat aaagttccca aaaacgtcat aatgaatcaa
     2221 ctgctggata agtacggtat caaaggtgaa aataccatgg ttgtcggaga cggacgctcg
     2281 gaaatacagg ccggggtgga tatgggtgct tttacaatga gcagattatg tactcatatg
     2341 cagtatcaga agcagcttca tatcaggatg ggtaccaacg taatagtgga agattttgaa
     2401 catgaggata tatacaagat attaattgcg aagtaaaaga ggagtagcgc ataaaagaga
     2461 ggactgtcag gtatttttaa cagttccttt tttatgtaat cgttttctct tcttttagct
     2521 tttccgggtc tctcattccc gaaaaaaaga aagaaatcat ttgtcatata cctgcttttc
     2581 agatatactc cgtttgtaaa ccgttatatt tttatgttat aaggagagat atggattttt
     2641 atcctgttga ttacttaaaa gaaaatatta gcgactccgg gtgcataaga gaatgcatag
     2701 agcaggctgt taaaatcaac ggtcataaga ggataatatt taacaaacgc aactggcata
     2761 tagatgaagc cattcttctg cacagcgata tggagattat agttgacggg tgttttataa
     2821 agcagaatga cggtgtgttt gacaatatat tcagaggagt caatttaaga gttgatgaga
     2881 aagacccgta tggtaaaccg ttgtatgtgg aaaaaatgaa aaatgtatcc ataaagggat
     2941 ataacaacgc aaagcttata gggtgtgatg tgaataaaaa agggtatcat cccgttaaga
     3001 aagagtatga ggatatggta ggggataact ggggatggag aacttttatg atatgcttat
     3061 ccgaatgtga aggcatagag atatcggggt taagtattga aaacactaaa tgctgggcgc
     3121 tgtcgctgga tttgtgttct tacggagata ttcatgatat ttttattcaa tcggatgtta
     3181 aaaacggaga cggtatcgat ttaagagcgg gatgccgtca tttccgtata tggaacataa
     3241 ccggcaatac atcggatgat acggtggcat gcactgcgct tttacatagc aagcaacggt
     3301 ttccgttcaa aaattatctg tatcctttag ctccggcggt ttccgtattt gacggagaca
     3361 tacgcaagct ggacatacat gatatttccg tttccgatat ccgcacggga ggtttgcacc
     3421 atgcggttat atgcctggca gccgacgggt tgagagtata taacatagat ataaagaata
     3481 tagaggacat aggggaaggt aacagatcgg caacggttat gttgtatacc gggtacggag
     3541 aaaattacgc ggataacgat attcatgaca taaatatata cggagttcat tcaagattct
     3601 ccaaatatgc ggtgcatata aacacaaaag tgcacaatgt caccgtatcc gatatcctac
     3661 aggaaaacaa agaaggcagt ctttacgagt ttgccgatac ggaaggtata acggtattaa
     3721 actaattaac tatatacgag tctattgaag gttgtgttat aaaagaaacc atataccaag
     3781 gcagataaag aattttattt tctcttttaa tgttgccgga atacagaact attgcttctt
     3841 tcatatcgta ttcctttaca gataaaagat tatccaaggc tttatgcaga ttatagtctt
     3901 taccggattt acattcgaca ggtaaggcaa tagcgccttt ttgtattata aaatcaattt
     3961 ctcctacttt tttactgttg tagtaataga gtttgaagtt atttgaaacc agtaattgcg
     4021 ctatggtgtt ttctaaaata cttcccatat taaccgaagt atcgccgttt agaacggcaa
     4081 attggatatt acccaaagac tgtgagcaca ataggcccgt atcacacagg aaaagtttga
     4141 aaagattata cttggtatta agttcaaaag gtaacaccgg ttgagatatg ttatagcagg
     4201 gaagtgaaac gcctgcgtcg gacaaccaca taaatccgct ttcgtatctg ttgtatctgg
     4261 catttttatc catgtctgat aaagtaaacc ttttattgtt tttattcaat tcagcaggta
     4321 tgtgatcaaa gatctgtctt attctcatct tttctgtttc ggagtacttg gttatatcct
     4381 gcctatatag ttccattatt cttttctgaa cagctatgac ctgtcctata tcttttgttt
     4441 ccgcaaattt agaaaccacg gcgggcatgc cgcctattat catatatctt tgaaacaact
     4501 tctttatctc ttcatgaact acttcactta tacacttttc attaataaaa cagtctttta
     4561 agtagtctat agtgtcttcc tgaacattgt tggcccaaag gaattcctca aaatccatgg
     4621 gatacatttg cagtatttct tcatatccta cgggaagtga aggtatttct ttatagttaa
     4681 ttccaaggag agagccggat tcaatatagt caaatctccc gtcttccaca aggaacttaa
     4741 ttgccgttct tgcatttgga cattcctgaa tttcgtcaag gaatataagg gttttaccgg
     4801 gcacaagagc ctttttcaca aaagccgaga ggtttagaat aatatcatca gctcttaagc
     4861 tgccgctgaa tatgttgtcg gcatttatgt ccgtaataaa atttatctct atgaagttat
     4921 cataatatgt tttgccgaat tgtcttatag atgccgtctt gccgacctgt cttgctccgg
     4981 taacaagaag agccgtctta ttattcgccc gtttccattt cagtaaatca ttttctactt
     5041 ttcttctcag catttcacac ctcttaacac tatatttgaa ttttatccgt atttaaggca
     5101 taagtcaata tgtgtgtaac gtttttctca tatattaagg agcattttgt aacgttattc
     5161 cgatataaag agggttgttt tgtaacgttt ttcccaagaa agagtatttg acataattct
     5221 cctcttgttc tatacttttt tggtaaggcg atgataagaa aaagtagtgt ggtcaatgga
     5281 tttacagaga gccttgatgc tgagataagg cattacacat cacatgaaac aggccttgga
     5341 gccgtgaaca ccgggggttc ccgttacagg acggaagtac ttttgtactt tataaggcat
     5401 atacggtgac ggatatgcga agaagggtgg caccgcgata ttatcgcccc tgataaaaca
     5461 ttagttttac gggggctttt ttgagatagg agttaattat gaaagaagaa attaaaagac
     5521 cggtatttcc caaaagggca gttataacat caggtatgcc gtacggaaac aaaggactgc
     5581 atttcgggca tataggggga ttattcgttc atgcggatgt ctttgccaga ttcatgcggg
     5641 acagaatagg ggaagaaaac gtcatcttca tatcgggaac cgactgttac ggctctccca
     5701 ttctggaaag ttacagaaaa cttaaagaaa gcggcaatac cgaacataaa aatatagttg
     5761 aatatgtaga gtccaatcat attaagcaaa aaaacacact ggatgcatat gaaataagtc
     5821 tcaatcattt cggagcatcg ggcttataca gagataaaga aattcacaga aaggtatcaa
     5881 ttgaagtttt tgaatcactg tacaaaggcg ggtatctttc tgttatgtct acgcctcagt
     5941 tttacgatcc gaaattcaag actttactga atggcagaca ggtggaaggc aagtgtccgt
     6001 ttgaagggtg cggatcggaa aaagcatatg ccgacgaatg cggactggga catcagtata
     6061 tgccggagga actgatcgat ccgaaaagca ctttatcggg attaaagccg gaaatagttg
     6121 atgtggagaa ctggtatttc cggctggaag atttccggga taagatgcag gagtatacaa
     6181 aagatttgag agagcactcc gttacaagga agtttgttct tgacgcaata gatgagtttt
     6241 tgaaacctcc cgtcatatat gttcagagaa aacagataga tgatttggaa gatctggaaa
     6301 gggctttccc cgagcacgaa acaatagatg aggaaaataa ggcttccatt acttttgttt
     6361 ttaagaatct tgatgaccgc gatattgcgc gcgatgtact gaatgaaagg cagatccgat
     6421 accgtaccgg caagactctg gttcctttcc gtctttcggg aaacatagac tggggagttc
     6481 ccattccgga aaaagataat ctgaaagacc ttactttctg ggtatggccg gaatcgttgt
     6541 gggcacctat atcatttttg agaacctatc ttgaagaaaa gggagcaaaa gaggatgaat
     6601 ggaaaaaatg gtggctggat gatgaaagca atgtatatca gttcatagga gaagacaaca
     6661 tatacttcta ctgcatagcg gagatggcaa tgtttgcggc aatgaagcat ccggattttg
     6721 atacattgga tatgaccaag cttcatcttc cagatattat tgccaataaa catattctgt
     6781 ttatggacaa aaaagccagc agcagttcac agataaagcc accgatggcg aatgatttat
     6841 tggaatacta taccgcagag cagttgagaa tgcattttat aagcttggga cttacaagta
     6901 agagcagctc cttcaagcct caggctttac ttcccaaaga agagcgggag ggagtggacc
     6961 ccgcattaaa agagggtaat cttcttacca atgttttcaa cagactgata agatcctgtt
     7021 tatattccat acagaaatac aacgattcga tagtaacgac aggaaaagta tcggaagaaa
     7081 tcaagcagga atcggatgat gcaatactgg aata
//
LOCUS       DAOPWC010000023         6981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_10780_length_6981_cov_3.776783, whole genome shotgun sequence.
ACCESSION   DAOPWC010000023 DAOPWC010000000
VERSION     DAOPWC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10780_length_6981_cov_3.776783"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..597)
                     /locus_tag="PKJ65_01025"
     CDS             complement(22..597)
                     /locus_tag="PKJ65_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNZ40450.1"
                     /translation="MIKRTLTADIETEIKVMITKDEYQILFSKGHNIHTQINHYYKIT
                     DDLTVRIRKINNEFFLQYKVSNDGVQLKGLKDKTEYSMKLSESDYLIIKNNPEALLTY
                     LKKEKTSDSSVVYKGTLETLRANVSLDVSMPDAEIDMNTYNGFTDYELEWEIDKKQYK
                     KALKILKNHGIDINDRVTGISKYKRLIQSYI"
     gene            complement(632..4054)
                     /locus_tag="PKJ65_01030"
     CDS             complement(632..4054)
                     /locus_tag="PKJ65_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ40451.1"
                     /translation="MVKWTNQQIKAIKTLNTNILVSAAAGSGKTATLIERIINILSDE
                     KKNVQCENLLVTTFTNAAAGEIRERIRRSLREKVQDNPSDLYIYKQYRNIDRATISTL
                     HSFCSEVIRQNAALINIDPGFSVADKTVADKFLLQAINTVYEQNLSNKDADFNRLLET
                     FGYGKNFNALTDLITSVLKTVKSYPDYHEWLRASAKNTLIGEDIFSSAYGKQIYDHIP
                     EKTDICKRYIDNMQYEISDTPEGEHYKATLENDLKIIEGIEDTLKYKDWDRLKNCLDA
                     IRFPNLPQKKKDFEEETVNAVKAYRTKLKKAVSALAALFYDYSDNIIKDCRFMQRPIE
                     KFVETVLQADDVYSKMKVKAKLIDYADMEHMALRCLKSGAEEIYREKYKYILIDEYQD
                     FNYIQEEIINNISRGDNVFAVGDLKQSIYRFRNAQTAIFRDKSEEYEDDPSSGTKLTL
                     SYNFRSRNAILKYVNDIFTDIMTEQLGEVDYNEDEELKYGSKQYENETDESYRPTVIK
                     ICVDHSELETQDRLSDVEIEAYVIADEIKQLIDSKLLIYDSKLKTSRPVRLSDIVILM
                     RAVSNYGDKYTEILNKCGISAYSEVSIEFLSNKTISDLYALLNVVDNPYQDIYLAQIL
                     KNKAYNFNECEFALIKKDKSDLYDALVSYNEEDRLQSKIQRFLEDFHDIRKFSRNNTV
                     EKTVYYAMTKSGIYYVSREQDLLKRFYVLAVECYKKGIDDLYGFLDFLKSKDKHELSV
                     IYDIMRQNKDNTVNIMSIHKSKGLEFPVVILAGSTRPFNFTDAQGRLIIDRKLGIGSD
                     YINCDNKTYHKHITKNALSLSMQKQSLSEEMRVLYVALTRAKEKLIITGIDKSFDEFV
                     IEQEAKCPFDWILPNSYSATFKTLNEKDCENIIRNLTEGEVAETVQARDLNDAAYYQN
                     ILAKTGFEYPYLEETRLNRKVTVTELSKYKSKRYDIDDFYNRYRVQTHKKPMFMQKDS
                     KISAAQKGTIMHKIMASIDLRQLNNPEYISYIKSKITEPEYLNNILAFIGTPLFNDIC
                     EADAIYQEKVFFMPVNTSYISAYTDDKYQSEHEVLLQGVIDCLIIKDGYATIVDYKTD
                     KVEKGNEKEHALRYMKQLDIYSEAVERIMGIKVTRKIIHFFETNTNVELNLL"
     gene            complement(4048..>6981)
                     /locus_tag="PKJ65_01035"
     CDS             complement(4048..>6981)
                     /locus_tag="PKJ65_01035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNZ40452.1"
                     /translation="TVNDTGKTMLLYSLLNKEELSLDYYQKSRKYPGFSVLAMQTIKE
                     LRANNISYEDILRISENGDFSYATKTKFAEIAAIYELYMTKMKEMGLADQTDSYDMLC
                     EHINNTTNFDNSIIWFDRFSFFSAKEFDVIKSLMNKAERINISLCIDYGISGPDSDIF
                     TIPLCTMDKLKEQAKQQRTTFIEERDKTYTEETVQSQSLDFLFSNLFRSRNEIRKGAV
                     KDIEIFKAYDIYEEAEYAAIRIMNAVENRGIRYKDILCVVSDVSSYERIIKAVFDQYD
                     IPFYINSKKSIADNPLIKFIISILDIYVWDYDIDSTSRFIKNLYSIIDEEDALKLENY
                     IIKWGISAKKAWHNVWDYENNAYDGRMNRLREKIVDSLDAFFNKVRYGCKPEEFARHL
                     YNLIENLRIYEKTDVEENYTESDGAEQAKQCYDSFIDILDQMVIMPDDEKRTVEYYMN
                     ILKTAFMETSVGITPVTKDCVILTNVARADIKDYDTMIVLGVNEGLFPGLSDSKSLFN
                     DSDIERMKQYGYEISTDPSQNAVKEEYQIYELMQSPTRKIYLSYHLHDSEGGDKNMSV
                     WLAKISAIFPNIEVLTRGDIPEYEYAHNIKTAFSMLFNGNTSLGDVCKDTRLEKLLYV
                     PSDTANITQSEMSGMLFGDTMNLSATMLERYVACPYSFLMAYGLKFDERPVFEYFSAN
                     LGSLKHSILFDAMSLICDDNIDYTYDEIYAICEQSAEKISRTNNIYKRDSVLEYVKKR
                     AVQQAADTITISLDIMKNEKMRPKYLETKFDENGIIKPLVFKTGNVNVRITGKVDRID
                     TATGANNEYFRVIDYKTSDNDIALYRIKEGLDIQLALYAYAYHKSVKAQLAGMYYMTI
                     EHKYAKVENSQDNSFEDINKVLPGYSFDDRSGSLSELIDNNTIKKSKVLDSDLQQNIF
                     QSVESTIVTSADNINNGNFAVKPLMDKKVSACKYCPYGMLCGFDSKKDRYRKVEAKKD
                     NEIIW"
BASE COUNT         1834 a         1448 c         1138 g         2561 t
ORIGIN      
        1 gttttgcttg tgtggccgtt ttcatatata actttgaatt aatcttttat attttgaaat
       61 cccggtaact ctgtcattaa tgtctatccc gtgatttttc agtattttca aggccttctt
      121 atattgcttt ttatctattt cccattccag ttcataatcg gtaaaaccgt tgtaagtatt
      181 catatctatt tcagcatccg gcatggatac atccagggaa acatttgctc ttaaagtctc
      241 cagtgtacct ttataaacta cactgctatc ggatgttttt tcctttttta gatatgtcaa
      301 taacgcttca ggattgtttt tgattatcag ataatcggat tcggacagtt tcattgagta
      361 ttcggtttta tctttcagac cttttaattg cacaccgtca ttgctcactt tatattgcag
      421 gaaaaattca ttattaatct ttcggatacg aacagttaaa tcatcggtta ttttgtaata
      481 atgatttatc tgtgtatgta tattatgtcc ttttgaaaaa agaatctgat actcgtcttt
      541 ggtaatcata acctttattt ctgtttctat atcagcagtt aatgtccttt ttatcattaa
      601 atacctttct ttacataagt aatcccgcca attatagcag attgagctca acatttgtat
      661 ttgtttcaaa aaaatgtatt attttacgtg tcactttaat tcccataatt ctttccaccg
      721 cttcggaata tatatccagt tgcttcatgt acctcaaagc atgttccttt tcattgcctt
      781 tttccacttt gtcggtttta taatcaacaa tagttgcgta tccgtctttt ataataaggc
      841 aatcaataac tccctgcagc agtacttcat gttcggattg gtatttatcg tcggtatatg
      901 cggaaatata gcttgtattt acaggcataa agaagacttt ttcctgatat attgcgtccg
      961 cttcacagat atcgttaaat aaaggggtgc cgataaatgc aagaatattg ttcagatact
     1021 cgggttctgt tatttttgac tttatatagg aaatatactc gggattattt aattgtctca
     1081 aatctatcga tgccattatc ttatgcatga ttgtgccttt ttgtgccgca cttatcttgc
     1141 tatccttttg cataaacatg ggttttttat gtgtttgcac acggtatcgg ttatagaagt
     1201 cgtcaatatc atatctctta ctcttatact ttgacaattc cgttaccgta actttcctgt
     1261 taagccttgt ttcttccaag taaggatatt cgaatccggt ttttgctaag atattctggt
     1321 aataagcggc atcatttaaa tcccttgcct gcactgtttc cgccacttcc ccttcggtca
     1381 gattcctaat gatattttca cagtcttttt catttaaggt tttgaatgtt gcactgtaag
     1441 aattgggaag aatccagtca aaaggacatt ttgcctcttg ctctattaca aattcatcaa
     1501 aacttttatc aatgccggtt attatgagtt tttccttagc cctggtcaag gctacataca
     1561 gaaccctcat ttcctcggat aaactctgtt tttgcatact tagtgagagt gcattttttg
     1621 ttatatgttt atggtatgtc ttgttatcgc agtttatata gtccgacccg atacccaatt
     1681 ttctgtcaat aatcagtctt ccctgtgcat ccgtgaaatt aaacggtcgt gtacttccgg
     1741 ccaatataac tacggggaat tccagtcctt tactcttgtg tatactcatt atatttaccg
     1801 tattatcctt attctgacgc attatgtcgt aaataacgga taattcatgc ttgtctttac
     1861 tcttgagaaa gtctaagaag ccgtataaat cgtcaatacc ttttttgtag cattcgactg
     1921 ccaatacata aaatctcttt aggaggtcct gctccctgct cacataatat attccggact
     1981 ttgtcatggc gtaatatacg gttttctcta ccgtattgtt cctggaaaac tttcttatat
     2041 catgaaaatc ctccaagaac ctttgtatct tagactgcaa tctgtcttct tcattgtaag
     2101 aaaccaaagc atcatacagg tcggatttat ctttttttat taaagcgaat tcacattcgt
     2161 tgaagttata tgctttgttc tttaatatct gggcaagata aatatcctgg tacggattat
     2221 caactacatt taaaagagca tacaaatcgg agatggtttt attacttaaa aactctatac
     2281 ttacctccga ataggcggaa ataccgcatt tattcaatat ttcggtatac ttatccccgt
     2341 aattacttac agctctcata agtatgacta tgtccgatag ccttacaggt cttgacgttt
     2401 tcagtttgct atcatatatc aacaatttac tgtcaataag ttgctttatt tcatcggcaa
     2461 ttacataagc ttcaatctcg acatcggaca gtcggtcttg tgtttccagt tccgaatggt
     2521 caacacatat tttaattacg gtaggtctgt aactctcatc ggtctcattc tcatactgtt
     2581 tggagccgta cttcaattct tcatcttcat tgtaatcgac ttctcccagc tgctcggtca
     2641 ttatatctgt gaatatgtcg ttaacatatt tcagaatggc atttctgctt cggaaattat
     2701 agcttaaagt gagtttagta cctgaagaag gatcatcctc atattcttcg gatttatctc
     2761 tgaatattgc ggtctgcgca tttctgaacc tgtatatact ctgcttcaag tcccctaccg
     2821 caaatacatt atcacctctg cttatattat ttattatctc ctcttgtata tagttaaagt
     2881 cctgatactc atctatcaat atatatttgt acttttccct gtaaatctcc tccgcaccgg
     2941 acttaagaca tcttaatgcc atatgttcca tatcggcata gtcaatcagt ttagccttta
     3001 ctttcatttt tgaatataca tcatccgcct gtaaaaccgt ttccacgaac ttttctatag
     3061 gtcgctgcat aaaccgacag tcttttataa tattgtccga atagtcataa aaaagtgcag
     3121 ctaacgcact tacggctttc tttaactttg ttctgtaagc ttttaccgca tttactgttt
     3181 cttcctcaaa atctttcttt ttttgcggaa gattcggaaa cctaattgca tccaaacagt
     3241 tctttaacct gtcccaatct ttatatttca aagtatcttc aataccttca attatcttta
     3301 aatcattttc cagtgtcgct ttatagtgtt cgccttccgg ggtatcggat atctcatatt
     3361 gcatattatc tatatatcgc ttgcatatat ccgttttttc cggaatatgg tcatatatct
     3421 gttttccgta tgcggaggag aaaatatcct cccctatcaa tgtatttttt gcacttgccc
     3481 ttaaccattc gtgataatcg ggataacttt taacggtttt taatacggaa gttataaggt
     3541 cggtcagtgc attgaaattc tttccgtatc cgaatgtttc cagcagtctg ttaaaatctg
     3601 catccttatt gcttaaattc tgctcataaa cggtattaat tgcctgtaac agaaacttat
     3661 ccgcaaccgt tttatcggca acggaaaatc ccgggtctat gtttataagg gcggcattct
     3721 gccttataac ttccgagcag aaggaatgaa gtgtacttat tgtagctctg tctatatttc
     3781 tatactgttt gtagatatag agatcggaag ggttgtcttg cactttttct cttaaggatc
     3841 ttctgatacg ttcccttatc tcacccgctg ctgcatttgt aaatgttgta accagaagat
     3901 tttcacactg aacattcttt ttctcatcgg aaagtatgtt aataatgcgc tcgataagtg
     3961 ttgccgtctt accgctgccg gcagctgcgg ataccagtat attggtattc agtgtcttta
     4021 ttgcttttat ctgctgattt gtccacttta ccatattatc tcattatcct tcttggcttc
     4081 cacctttcgg tatcggtctt ttttactgtc aaaaccgcac aacatgccgt aggggcagta
     4141 tttacatgcc gatacttttt tatccattaa aggcttaact gcaaaatttc cgttgtttat
     4201 attatcggct gatgtaacta tggtgctttc tacgctttga aatatatttt gctgtaaatc
     4261 gctgtccagt accttacttt tctttatcgt gttgttgtct ataagttcgg ataagctacc
     4321 cgaccggtca tcaaaggaat accccggcag caccttattg atatcttcaa aagagttatc
     4381 ctgactgttt tccaccttgg catatttatg ttctatagtc atgtagtaca tacctgccaa
     4441 ttgcgctttt accgatttat ggtaagcata agcatatagg gcaagctgta tgtccagccc
     4501 ctcttttatt ctgtacaagg cgatatcgtt atccgatgtt ttgtaatcta ttactctgaa
     4561 gtactcgtta tttgcgcccg ttgcagtgtc aattctgtcc accttaccgg taatgcgtac
     4621 attaacattg cccgttttaa atacaagagg tttgattatg ccgttttcgt caaacttggt
     4681 ctccagatat ttcggtctca ttttctcgtt tttcataata tccaaagaaa ttgtaatggt
     4741 atccgctgct tgttgaaccg ccctcttctt tacatattcc aataccgaat cgcgtttata
     4801 aatattattt gtcctgctta tcttttccgc cgattgctcg cagattgcat atatttcatc
     4861 atatgtataa tcaatgttat catcacatat caaactcatg gcatcaaaca gtatcgagtg
     4921 ttttaacgag cctaaattgg cggaaaaata ttcaaacacc ggcctctcat caaatttcag
     4981 cccgtatgcc attaaaaagc tgtacggaca tgccacatag cgctccaaca ttgtggcgct
     5041 taagttcatt gtgtctccga aaagcatgcc ggacatttcc gattgagtaa tgtttgcggt
     5101 gtcggaaggt acatacagta atttttccaa tcgggtatct ttacaaacat cccctaaaga
     5161 agtattaccg ttaaacagca ttgaaaaggc cgttttgata ttatgtgcat actcgtattc
     5221 cggtatatcc cctctggtta aaacttcaat attggggaat atagcggaaa tctttgccag
     5281 ccagaccgac atattcttgt cgccgccctc tgaatcatgc agatgatagg acagatatat
     5341 ctttcttgta ggactttgca taagttcata tatctgatat tcctctttga cggcattctg
     5401 tgacggatcg gttgatatct cataaccgta ttgcttcatt ctttcaatat ccgaatcatt
     5461 gaataaagac ttggagtcgc tcaatccggg aaacagtcct tcattaactc ccaagacaat
     5521 catggtatcg taatctttta tatcggctct tgctacgttg gtcagtataa cgcaatcttt
     5581 tgtgacggga gtaatgccaa cggaagtttc cataaacgcc gtttttaaga tattcatgta
     5641 gtattccacg gttctctttt catcatccgg cataataacc atttggtcta agatatctat
     5701 aaaagagtca tagcactgtt ttgcctgctc cgctccgtca ctttcggtat agttttcttc
     5761 tacatcggtc ttttcataaa tacgcaagtt ttctataaga ttgtataaat ggcgtgcaaa
     5821 ttcttccggt ttacagccgt atcgtacttt attaaaaaaa gcgtccagag aatcaacaat
     5881 tttctctctt aatctgttca ttctgccgtc ataggcattg ttctcgtaat cccagacatt
     5941 gtgccatgct tttttagcgg aaatacccca ttttatgatg tagttctcca gtttaagagc
     6001 atcctcttca tcaattatcg aatagaggtt ttttataaat ctcgaagtac tgtctatgtc
     6061 ataatcccag acgtaaatat ccaagatgga aataatgaac tttattaacg ggttatccgc
     6121 aatgctcttt ttggagttta tgtagaaagg aatatcgtat tggtcgaaaa ccgcttttat
     6181 tattctttca taggaagaaa catccgatac cacgcaaaga atatccttgt atcttatccc
     6241 tctgttctcc acggcattca ttatcctaat agccgcatat tccgcttcct cgtatatatc
     6301 atatgcctta aaaatctcaa tatcctttac tgctcctttt cttatttcat ttcttgaacg
     6361 aaacagattg gaaaaaagaa agtccaatga ctgagattgc actgtttctt cggtatatgt
     6421 cttatcccgc tcctctataa aagtagttct ctgttgcttt gcctgttctt tcagtttgtc
     6481 catggtgcat aacggtatgg tgaatatatc gctgtcggga ccgcttattc cgtaatctat
     6541 gcataaagag atgtttattc tctcggcctt attcataagg ctttttatga catcaaactc
     6601 ttttgcggaa aaaaatgaaa acctgtcaaa ccagatgata ctgttgtcaa agtttgttgt
     6661 attgttaata tgctcgcata acatatcata ggaatccgtt tggtccgcca atcccatctc
     6721 cttcatctta gtcatgtaaa gctcatatat agcggcaatt tcggcaaatt ttgtttttgt
     6781 cgcataggaa aaatcaccgt tctcacttat acgcagtata tcctcataac ttatattatt
     6841 tgctctaagc tcttttatgg tctgcatcgc aagaacggaa aaaccgggat attttctgga
     6901 tttttgataa taatccaggg acagttcttc tttattcagt aaagaataaa gtaacatggt
     6961 ctttccggta tcgtttacgg t
//
LOCUS       DAOPWC010000024         6884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11004_length_6884_cov_3.780641, whole genome shotgun sequence.
ACCESSION   DAOPWC010000024 DAOPWC010000000
VERSION     DAOPWC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_6884_cov_3.780641"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKJ65_01040"
     CDS             complement(<1..463)
                     /locus_tag="PKJ65_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40453.1"
                     /translation="MNEYLHAIKIKWRNSAFVRTFNLHEEKAKGRSCFLASSLLLSLV
                     TQLSGGIFYTGFLLSFGINIVNIGIITFVPIITKLLSIFSPFILERFRKRKVFLTAMS
                     ILANVINILGVTLLPDIITSEAGRVIGFTLLVTTSSAISAIIDPGYSAWHAP"
     gene            complement(539..1888)
                     /locus_tag="PKJ65_01045"
     CDS             complement(539..1888)
                     /locus_tag="PKJ65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40454.1"
                     /translation="MNEYLHAIKIKWRNSAFARTFNLTDEKAKGRSCFLTSSILASLA
                     AQLSGGVFYTGFLLSFGINIVNIGIITFVPVVTRLLSIFSPFILERFRSRKVFLTVMG
                     ILANIINILGVTLLPNIITSETGRVIGFTLLVTISSAISAIIDPGYSAWHAPYLPNEI
                     RGDYFTLSSGVNSFLTQGVVLILSLVADSFAGSPRQLEIITGFRYAALVLAVMHILVL
                     AIPKEYPYEQSGSKIKLSNIFVLPFKSKKFLWTVILIGFYYLGASLTNASINIFLLQD
                     VGVTYSQINLINAVYFMFFIFFGKMWKKVLKKYSWFKTLAIAMLLQGPTFIMYAFVTA
                     ENYTWLWFTVRLLQHVLGVVSNTVIASMLYVNLPDTDRTYYVSFYNIVIHMSSFLSMM
                     LGTMFLSWMGDNVLNVFGFKMAGVPVLLAITGIAEIVVGILSWKFNDKLLPDDLTKR"
     gene            2116..2508
                     /locus_tag="PKJ65_01050"
     CDS             2116..2508
                     /locus_tag="PKJ65_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HNZ40455.1"
                     /translation="MKKDQNRFFICRRCGNMIGMIYNSGVPIVCCGVNMEQLVANTTD
                     GANEKHLPVIKQNGNNVVVEVSTVAHPMVKEHYIMWIYLETAKGGQRKALLPGEKPVA
                     TFAITDDDKVIAAYEYCNLHGLWKTAVK"
     gene            2548..2706
                     /locus_tag="PKJ65_01055"
     CDS             2548..2706
                     /locus_tag="PKJ65_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNZ40456.1"
                     /translation="MKKYVCMVCGYVYDEKEGDPDNGVDPGTKWEDVPEDWTCPICGV
                     SKEEFEEE"
     gene            2859..3920
                     /gene="aroB"
                     /locus_tag="PKJ65_01060"
     CDS             2859..3920
                     /gene="aroB"
                     /locus_tag="PKJ65_01060"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNZ40457.1"
                     /translation="MRKITVAGSKKYDIIIDKNILSEAYQYIKPFLTGNIVYIVTDDI
                     VDSLYGSILTDSLESNGLSVFRYIIPHGEQSKNTDNYIAVLNDMATKGVSRSDMLIAF
                     GGGVPGDFGGFAAATYLRGIPLLHIPTTLLATVDSSIGGKTGINLKEGKNLAGAFYQP
                     QAVICDLSLLDTLPDEVFKDGYAEIVKYGILSGNQLFNRIKKPLKSDIESIVYECVKI
                     KRDIVQKDESDIGMRQLLNLGHTIAHAVELLSGYAISHGAAVAKGLHLISKISYTQGL
                     CTKEVLTKITDMLNLHGFNTDLLYGAKDIANAITADKKRSSGHINLILIRDIGKCTIE
                     KTDMSDLTDIIQTATEYRG"
     gene            3922..5184
                     /gene="aroA"
                     /locus_tag="PKJ65_01065"
     CDS             3922..5184
                     /gene="aroA"
                     /locus_tag="PKJ65_01065"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ40458.1"
                     /translation="MDISVTVKNPGGTVKAITSKSHAHRHLICSALSDVPNFVEVTDT
                     SSDIDATVQCLNSLGANITAEKNGFYINPIKTVRINALLDCNESASTFRFLLPVTCAL
                     GAKSSFIMKKGLSKRPITPLYNQLIGKGCTLSLEGTVPFLTEGQLKSGIYQLPGDVSS
                     QYISGLLLALPLLKNKSGILLTSDLQSSGYVDMTADVMNQYNVKIIKTEKGFEIPGNA
                     TYTPTGNMSIEKDWSNAAFFLCAGAFSERAITVTGLNIHSRQTDKTIIDILKTTGAYV
                     TVNKDSVTVKKNRLNATDIDASQIPDLVPVLALLAAVSEGTTNIFNAKRLKLKESDRL
                     SSTCDMLKTLGADITEKPDSLVITGKEMLNGGTVCSHNDHRIAMTAAIASIVCENAVI
                     IKEAHAVNKSYPAFFRDFASIGGITERI"
     gene            5189..6280
                     /gene="aroC"
                     /locus_tag="PKJ65_01070"
     CDS             5189..6280
                     /gene="aroC"
                     /locus_tag="PKJ65_01070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ40459.1"
                     /translation="MSSVFGKNITVTIFGQSHSKAVGVVIDNLPAGFSIDMQKLTAFM
                     QRRSAIDKPLSTKRKEKDEIEFLSGLVGNTTCGAPICAIIYNKDINPEDYRSFKDIPR
                     PSHADYTAHIKYKGFQDTSGGGHFSGRLTAPLCIAGGICLQILEKYGISIHAHIKKIY
                     DTEDISFNPAEDNRENIEKLLNNDFPVIDRQKGILMQERIRTASQQNDSLGGIIECGV
                     YNLPAGIGEPMFDGLENNISKAVFAIPAVKGIEFGNGFDCTNLKGSENNDPFYIKDSK
                     ILTRTNNHGGILGGISSGMPLIFRVAVKPAPSIGIKQESVNMANNNSEQIKVGGRHDA
                     CIVPRAVACVEAAAAIAILDMYASYRIWE"
     gene            6306..>6884
                     /locus_tag="PKJ65_01075"
     CDS             6306..>6884
                     /locus_tag="PKJ65_01075"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNZ40460.1"
                     /translation="MNLDDLRKEIDKIDDKLLQLFQERMGIVKDIAEYKKVHNIPILN
                     SELEKVKLSDIISKTDDELVSYTQILFNTLFDISKSYQEKLNISKPAALGYSDKKFGL
                     LGAELQHSYSPLIHSHLGDYEYGLYEKQPDQLYDFVHNSDLDGFNVTIPYKKDIMKYC
                     NILTPIAKRTGSVNTVIGRANNLTGDNTDYYGF"
BASE COUNT         2337 a         1332 c         1365 g         1850 t
ORIGIN      
        1 acggtgcatg ccaggcggaa taaccgggat cgattattgc ggatattgct gaagaggttg
       61 ttaccaataa tgtaaagcct attactcttc ccgcttccga tgtaataatg tcgggcagca
      121 atgttacacc caatatgttt attacattgg ctaaaatact cattgccgta agaaaaactt
      181 ttctctttcg gaatctttcc aatataaaag gagagaatat cgacaacagc tttgttatta
      241 tgggaacaaa ggttattatc cctatattaa caatgtttat tccgaaactt aagagaaaac
      301 ctgtatagaa tattccgccg ctcagctgtg tcactaatga taaaagaaga gatgatgcca
      361 aaaaacaact tcttccttta gccttttctt catgcagatt aaatgtcctt acgaaagccg
      421 aattccgcca ctttatcttt atcgcatgca ggtactcgtt cataaattcc tcaaccggtt
      481 aatgcctgtg cattctataa tactgattat accaagtcaa cgggaatatt ggtaacaatt
      541 atctcttcgt taagtcatcg gggagcaact tatcattaaa tttccaggaa agaatcccca
      601 caacaatctc cgctataccg gttatggcaa gcaatacggg aacacccgcc attttaaagc
      661 cgaaaacatt taagacattg tctcccatcc atgaaaggaa catggtgccc agcatcatgc
      721 ttaagaaaga ggacatatgt attactatat tatagaagga aacataatat gtcctgtccg
      781 tgtccggaag atttacatac agcattgaag caattacagt attggagact actccgagca
      841 catgctgcaa cagtcttacc gtaaaccaga gccaggtata gttttccgct gtgacaaaag
      901 catacataat gaatgtcggt ccctgtaaaa gcattgcaat tgcaagagtc ttaaaccagg
      961 aatacttttt aagaaccttt ttccacatct ttccgaaaaa gataaaaaac ataaaatata
     1021 cggcattgat aaggttaatc tgagaatagg ttacacctac atcctgaagt aaaaagatgt
     1081 taatggaggc atttgtaaga cttgctccca gatagtaaaa gcctatcagg ataacagtcc
     1141 acagaaattt tttactctta aaaggcagaa cgaatatgtt gcttaatttt atcttgctgc
     1201 cgctttgctc ataaggatat tccttaggta tagccaatac cagtatatgc attacagcca
     1261 ataccaatgc cgcataacgg aatcccgtta ttatttccaa ttgcctgggg ctgcccgcaa
     1321 aggagtctgc taccaatgaa agaatcaata ccacgccctg cgtcaaaaag ctgtttactc
     1381 cggagcttaa tgtgaaataa tcccctctta tctcattggg aagatacggt gcatgccagg
     1441 cggaataacc gggatcgatt attgcggata ttgctgaaga tattgttacc aataatgtga
     1501 atcctattac tcttcccgtt tccgacgtaa taatattagg tagtagcgtt acacctaata
     1561 tatttattat gttggccaat atacccatta cagtaagaaa aacttttctt gaacggaatc
     1621 tttccaaaat aaaaggagag aatatcgaca gcaaccttgt aacaacggga acaaaggtta
     1681 ttatccctat attaacgata ttaattccga aacttaagag aaaaccggta taaaacactc
     1741 ctccgcttaa ctgtgcagct aaagaggcaa gtatggacga agttaaaaaa caacttcttc
     1801 ctttagcctt ttcatcagta aggttaaatg ttcttgcaaa agcggaattc cgccacttta
     1861 tctttattgc atgcaggtac tcgttcataa atccctcaat cagctaatat tctgagtatt
     1921 ctacaatact gattatataa agtcaatgag aacattggta acaattatgg acactttatg
     1981 cgaatttatg gaactgataa atacagggaa cctttgtgta caaaatatat ttgcaaataa
     2041 cctatagaca actatatgtt tgtgatataa taaaagtcta atagtactaa gtactaaata
     2101 gaaaggagaa atacaatgaa aaaagatcag aacagatttt ttatctgcag acgttgcgga
     2161 aatatgatag gaatgattta taactcgggt gtgcccattg tctgttgcgg agtgaatatg
     2221 gagcaattgg ttgcaaatac aaccgacggt gcaaatgaaa aacacctgcc tgtaataaaa
     2281 cagaacggca acaatgtagt tgtggaagta tccaccgtag ctcatcccat ggtaaaagaa
     2341 cactatatca tgtggattta tctggaaacg gcaaaaggcg gacaacgtaa agctcttctt
     2401 ccaggggaaa aacccgtggc aacatttgca atcaccgatg acgacaaggt aattgcggca
     2461 tatgaatact gcaatctgca cggactgtgg aaaactgccg taaaatagtg tatagtcata
     2521 aatataattg tggaggaggt tttttccgtg aaaaaatatg tttgcatggt atgcggttat
     2581 gtttatgatg aaaaagaagg cgatccggat aacggtgtag atcccggcac aaaatgggag
     2641 gatgttccgg aagactggac atgcccgata tgcggagttt ccaaagagga gtttgaagaa
     2701 gaataatata aacatcaact cgaagaaagt agcacatgct actttctttt tttagagcct
     2761 gctaagaaca taatcatatt atcattatac ggtttgttaa taaacataac ttcaaaccgt
     2821 tagatgataa tgtataatag tgacatggcg gatacaagat gagaaaaata actgtagcag
     2881 gttcaaagaa atacgacata ataatagata aaaacattct gtcggaagca tatcaatata
     2941 ttaagccttt cttaacaggt aatattgtct atatcgtaac cgatgacata gtggattcgc
     3001 tatacggctc tatattaacc gactctttgg aaagcaacgg gctgtcggta tttaggtata
     3061 ttattccgca cggggagcaa tccaaaaaca ccgacaatta catagcggta ctcaatgata
     3121 tggcaactaa aggagtttcc agatcggata tgcttatcgc tttcggtggc ggggttcccg
     3181 gtgatttcgg cggctttgct gcggctacat acctgcgcgg tataccgctt ttacacattc
     3241 ccaccacatt gcttgcaaca gtggattcct caataggagg aaaaacggga attaatctaa
     3301 aagaaggcaa aaacctcgca ggtgcttttt atcagccgca agcggtaata tgcgaccttt
     3361 cattattgga tactttaccg gatgaagtat ttaaagacgg atatgccgag attgtaaaat
     3421 acgggatatt atcgggcaat cagcttttta accgtataaa aaaacctcta aaaagcgata
     3481 tcgaaagcat cgtatacgaa tgtgtaaaaa taaaaagaga cattgtacaa aaagatgaat
     3541 cggatatcgg tatgcgacaa cttcttaatc tgggacatac gatagctcat gctgttgaac
     3601 ttctgagcgg ttatgcaatt tcgcacggtg ctgcggttgc aaagggactg catttaatat
     3661 ctaaaatttc ctatacacaa gggttatgca ccaaggaagt tttaactaag ataacagata
     3721 tgctgaacct ccacggtttt aataccgatt tgttatacgg cgcaaaagat atcgcaaacg
     3781 caataacggc ggataagaaa agaagcagcg gccacataaa tctgatactt atacgggaca
     3841 taggtaaatg cactatagaa aaaaccgata tgtcagattt gaccgacata atacaaaccg
     3901 caacggaata tagaggttga aatggatata tcggttacag taaagaatcc ggggggtacc
     3961 gtaaaagcga ttacatcaaa atcacatgca cacaggcatc tcatatgttc ggcattgtca
     4021 gatgtaccta actttgttga agtaaccgac acctcttccg atattgatgc taccgttcaa
     4081 tgccttaact cattgggtgc aaacattacc gctgaaaaaa acggttttta tataaacccg
     4141 ataaaaaccg taagaataaa tgcccttttg gactgcaatg aatcggcctc cacttttcgt
     4201 ttccttcttc cggttacctg tgcattggga gcaaagtctt cctttatcat gaaaaaaggg
     4261 ttatccaaaa gacccataac acctttatat aatcagctta tcggaaaagg ctgcacatta
     4321 tctttagaag gaaccgtacc tttccttacc gaagggcagc ttaaaagcgg tatatatcaa
     4381 ttacccggtg atgtatcttc tcaatatata tccggtcttc ttctggcact tcctcttctg
     4441 aagaataaaa gcggcatcct gctaacctcc gaccttcagt cttcaggata tgttgatatg
     4501 actgctgatg tcatgaatca atacaatgta aagattatta aaacagagaa aggttttgaa
     4561 atacccggca atgctactta tacaccgaca ggaaatatga gtattgaaaa agactggtcc
     4621 aatgcggcat tcttcctttg tgccggagct ttctcagaaa gagcaattac cgttaccgga
     4681 ttgaatatac actcccgaca aaccgacaaa acaattatcg atattcttaa aacaacggga
     4741 gcatatgtta cagttaataa agatagtgta accgtcaaaa agaaccggtt aaatgccacc
     4801 gatattgatg catcacaaat acccgacctt gtccccgtac ttgctctttt ggcagctgtt
     4861 tccgaaggaa caaccaatat attcaatgca aaaaggctta agttaaaaga aagtgacaga
     4921 ttatcttcca cttgtgatat gcttaaaacg ttaggtgctg atataacgga aaaacccgat
     4981 tccctggtta taacaggaaa agaaatgttg aacggcggaa cagtttgttc ccataatgac
     5041 cacagaattg ccatgaccgc ggcaatagca tctattgtgt gtgaaaatgc cgtaataatc
     5101 aaagaggcgc atgcggtaaa caaatcatat cccgcctttt tccgggattt tgcgtctata
     5161 ggcggtataa cggaaaggat ttagtaaaat gtcatcggta ttcggaaaaa acataacagt
     5221 cactatattc ggacaatcac attcaaaagc tgtaggtgtg gttatcgaca atctgcctgc
     5281 aggtttttca atagatatgc agaaactcac cgcttttatg caaagaagaa gtgccataga
     5341 taaaccgctc tccacaaaaa ggaaagaaaa ggatgaaata gagtttcttt ccggacttgt
     5401 gggaaatact acatgcggtg ccccgatatg tgcaattatt tacaataaag atataaatcc
     5461 cgaagactac cgatccttta aggatatccc cagaccttct catgccgatt atacggctca
     5521 tataaaatac aaaggttttc aggacacttc gggaggagga cacttctcgg gaagactgac
     5581 cgcaccttta tgtatagcgg gaggtatctg tcttcagata ctggaaaagt acggtataag
     5641 catacatgca cacataaaaa agatatacga tacggaagat atatctttca atcctgcaga
     5701 agataacaga gagaatattg aaaaactctt aaacaatgac tttcccgtta tagacaggca
     5761 aaaaggcata cttatgcagg agcgcattcg tacagcatca caacagaatg attcgttagg
     5821 cggaataata gaatgtgggg tatataacct tcccgcagga ataggcgagc ccatgtttga
     5881 cggtctggaa aacaacattt ccaaggccgt gtttgcaata cccgcagtaa aaggaataga
     5941 gttcggtaac ggttttgatt gcaccaatct gaagggctcc gagaacaatg atcctttcta
     6001 tatcaaagac agcaaaattc ttacgcggac aaacaaccac ggcggcatat tgggaggcat
     6061 aagctccggg atgccgttga tattcagagt ggccgtaaag cccgctccgt caataggtat
     6121 taagcaggaa agtgtcaata tggcaaacaa taacagtgag caaataaagg taggcggaag
     6181 acatgatgca tgtatcgtgc ccagagcggt tgcctgtgtg gaagcggcgg ctgcaatagc
     6241 aatattagac atgtatgcat catacagaat atgggaataa tttaaaaatt atataaaggg
     6301 ataaaatgaa tttagacgat ttgagaaaag aaatagataa aattgatgat aaacttttac
     6361 aattatttca ggaaagaatg ggcattgtaa aagacattgc ggagtataaa aaagtacaca
     6421 acatacccat acttaacagt gaattggaaa aagtcaaact ttcagatatt atctccaaga
     6481 cggacgatga gttggtctcc tatacacaaa tactcttcaa tacattgttt gatataagca
     6541 aatcttatca ggaaaagctg aatatttcta agccggctgc actcggatat tccgataaga
     6601 agttcggttt attgggtgcc gaattacaac acagctactc cccgcttata cattcccatc
     6661 tgggcgatta tgaatacggg ctttatgaaa agcagcccga tcagctttat gattttgtac
     6721 ataacagcga ccttgacggt tttaacgtaa cgatacccta taaaaaagat attatgaaat
     6781 actgtaatat actgacacct atcgcaaaaa gaaccggcag tgtcaatacc gttataggta
     6841 gagcaaacaa tcttacagga gataataccg attactacgg tttt
//
LOCUS       DAOPWC010000025         6867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11035_length_6867_cov_3.860393, whole genome shotgun sequence.
ACCESSION   DAOPWC010000025 DAOPWC010000000
VERSION     DAOPWC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11035_length_6867_cov_3.860393"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..511)
                     /locus_tag="PKJ65_01080"
     CDS             complement(98..511)
                     /locus_tag="PKJ65_01080"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNZ40461.1"
                     /translation="MGRAFGEPLTEKLAGGGKTKKVVDFVKRNGPIFFLLAFLIPGLP
                     DDLLCYVAGIVMLPFGIFLFLALFARILSTLSLAFLGDGVLKWTTGQMILVIVIMLVL
                     IGLVIIFRKPITNFLNMLVRKESKDDDVDITDMMT"
     gene            complement(512..847)
                     /locus_tag="PKJ65_01085"
     CDS             complement(512..847)
                     /locus_tag="PKJ65_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40462.1"
                     /translation="MSEDKKKKIRRRNIKLIIASAIILALLIFILTEKGKQLWDLFQL
                     NGEQFKEYIESKGKWGPALFFLLSYLQVVIAPIPGQITYFVGGMLFGTWGGFAVNHTA
                     TVLASITVY"
     gene            complement(844..1695)
                     /locus_tag="PKJ65_01090"
     CDS             complement(844..1695)
                     /locus_tag="PKJ65_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ40463.1"
                     /translation="MIITGISDEAGKHITTQIEAHKKLGWNTLELRNHNGVNPSTTKF
                     SDQEFDNVRKIIEEHNFKVTAFSSAIGNWSRSIRGDFNIDKEDLKISAKRMKMLNVRY
                     IRIMSWIPDEDDKTYTKKEVIRRCKELAKMAEDLDIVLAHENCTGWGGEAPENMLELK
                     QEVNSPNFVLLYDTGNVVAHGYDKPMKFFQGLRGHIDYVHIKDALIAKPGLENQFRYV
                     GEGEGKVREILDILINEDKYDGVISIEPHVASIVHTGEKPAGDEELFRSYIKYGNMLK
                     DMIASVK"
     gene            complement(1916..2308)
                     /gene="rsfS"
                     /locus_tag="PKJ65_01095"
     CDS             complement(1916..2308)
                     /gene="rsfS"
                     /locus_tag="PKJ65_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ40464.1"
                     /translation="MDTENFKNEIVEVLKEKKAKDLISIEVRDVTTLTDYFVICTGTS
                     DRHRKSMADDVQEKAEALGMTVSMPVEGYNQAKWILIDCMDVVVNIFEKETRSRYNLE
                     KLWSDGVLEEIRENNNKEVLSSTENSAV"
     gene            2527..3102
                     /locus_tag="PKJ65_01100"
     CDS             2527..3102
                     /locus_tag="PKJ65_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40465.1"
                     /translation="MVYWGTQFLVKKPHMLGSKLDDKIPFIPVFIFPYVIWFVLLFTI
                     QFVLYFYSLQTYALYVMSLLIVDITSGIIYLVYPTTFIRPEPEGDSFSVKVVRFIYSH
                     DKKILSCLPSLHCSLSTLFILSALYATGLPLFLKLIIIIFSLLVIVSTVFVKQHVIID
                     VITAIPLAVISWYIAMAIGADTFLTFFNFIQ"
     gene            3163..3591
                     /locus_tag="PKJ65_01105"
     CDS             3163..3591
                     /locus_tag="PKJ65_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40466.1"
                     /translation="MKTAIIYYSYGGKTKKYCENMAAELSADILEVQTVKRKCFLSNL
                     FTECPKALQQQPTDILPIDLDLSGYEKIILAAPMWAGFPAPPFNNMVAMLPKGKQVEI
                     YIVSASGETDPIKKEKVIVLTEETGSKVIKYTDICGADIK"
     gene            complement(3593..4348)
                     /locus_tag="PKJ65_01110"
     CDS             complement(3593..4348)
                     /locus_tag="PKJ65_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40467.1"
                     /translation="MWAQNNAKRWVIMSIIFMLIGIAMIVAGAVIGEMNYYWMIYVGA
                     ILFLTFFICFFMFIRQARNLDSMLKGTGLLAHWRFEENERLQRAAEEFLQRKTSNKVM
                     LLVIIAFVVIIGGLFAIFGFGNSKDAIFFIVVLLGIQILISLAAILIPRARYNKMKEA
                     APEVLISPFGAWIMGEYFQWKAPLTRITSVQFAKDREDNYTITVNFTIWQRFGPQKLQ
                     CRIPVPKDTVTEAETIASDIAKINKVRFLNETK"
     gene            complement(4352..5311)
                     /locus_tag="PKJ65_01115"
     CDS             complement(4352..5311)
                     /locus_tag="PKJ65_01115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ40468.1"
                     /translation="MYTGIFKNAYKVIKTEEGYFPVRFTDKQLEYYKNLSKATEDRSL
                     CTAGICMDFYTDAECITFSYRADHFSRRYVGFDFYENGIFKRHIEEALDTKQSTLTYP
                     LTEKGIKRVTVYVPNLSRIRVYDLDIGNFQCIKEQPSQKLLFLGDSITQGMVALSPSL
                     AFPTQVARALNAQSLNRGVGSAKFDVDSLDEQDKYDPDSIFIAYGINDIYQLKDMAEL
                     ENVLKTADKYLYKLKNIYKHAKVYIITPIWCLRYDEDQSFKEMFDEYQKELTTLAKKY
                     GFIPVNGLALMPHDTKYHQDHTVHPADSGFNHIAENLLKIIGR"
     gene            complement(5311..5886)
                     /gene="yqeK"
                     /locus_tag="PKJ65_01120"
     CDS             complement(5311..5886)
                     /gene="yqeK"
                     /locus_tag="PKJ65_01120"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNZ40469.1"
                     /translation="MGYISEEARLDIVKRYSPERYEHVKNVVELADHLARLHRADSEK
                     VYAAALLHDIAKDMKTEDMAAIIEAAGIFIDKQYHTGNILHGPCAAVIAKKEYNIEDE
                     EILNAIYYHSFGRAGMGLTEKIVFIADAAEKGRTYKNVTKIRKLMYNKLDEAVELSLD
                     GTVYHVLSKGEVLYPVTAAALEYFKEQRIKD"
     gene            complement(5870..6469)
                     /gene="nadD"
                     /locus_tag="PKJ65_01125"
     CDS             complement(5870..6469)
                     /gene="nadD"
                     /locus_tag="PKJ65_01125"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNZ40470.1"
                     /translation="MKLGILGATFDPVYKAHILMAKYALDYCEYVLLTPCTKPPHKDN
                     AGITDDNIRYTMLCLALDGYEKLIASDMELKRATTTYTIDTLRQVKELYPVFDEIIYI
                     IGADTLFQLKTWREYEEVFKLCTFLVFGRSGFTEEQITEQIEIFKKDYGADIIFDIRS
                     ISEISSTAIRKGIAEQDNSIEWMLDKKVYRYIKENGIYK"
     gene            complement(6466..>6867)
                     /locus_tag="PKJ65_01130"
     CDS             complement(6466..>6867)
                     /locus_tag="PKJ65_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNZ40471.1"
                     /translation="ARFGDPETQVLLPLLKTDLLDIFNAITDERLDKTDIQWENSAGA
                     TVVLASGGYPKEYTKGYPISGLEQVQKQGITVYHMGTAMKDGEYVTNGGRVLSLTAKG
                     DTVEQAVARVYNNINKVCFKDMHYRKDIGRK"
BASE COUNT         1872 a         1512 c         1161 g         2322 t
ORIGIN      
        1 tcccgaacca agcgcgctac caaactgcgc tacaccccgt gcttagatat tataacccgt
       61 cttttgcgtt ctgtcatctg ctatttttta atattcttta tgtcatcata tccgttatat
      121 ccacatcatc gtctttgctt tcttttctta cgagcatatt gaggaaattg gttatgggtt
      181 ttctgaatat tattacaagt cctataagaa caagcattat gacaataacc aatatcatct
      241 gtcccgttgt ccatttgaga acaccgtctc ccaaaaatgc caaggataga gttgagagta
      301 ttcttgcaaa taatgctaag aaaaggaata ttccgaaagg caacataact atcccggcaa
      361 cataacataa aaggtcatcg ggtaatcccg gaataaggaa tgctaaaagg aagaatatag
      421 gaccgtttct cttgacaaaa tccacaacct tttttgtctt tcctcctccc gccaactttt
      481 cggtaagagg ctctccaaac gctctcccca atcaataaac ggttattgat gccaatactg
      541 ttgccgtatg gttgacggca aaacctcccc aggtaccgaa cagcataccg cctacaaaat
      601 atgttatctg acccggtatg ggagcaatta caacctgtaa gtaggaaagc agaaagaaca
      661 gagcgggacc ccatttgcct ttgctttcaa tgtattcttt aaactgttca ccattcagtt
      721 gaaacaagtc ccacagttgt tttccttttt cagttaagat aaagataagt aaggctaaaa
      781 taatagcaga tgctataata agctttatat tccttcttct tatctttttc tttttatctt
      841 cactcatttt acagatgcaa tcatatcttt aagcatgttt ccgtatttta tatatgaccg
      901 gaaaagctct tcatcgccgg cgggtttctc gcctgtatga acaatagagg ctacatgagg
      961 ttcaatggat ataactccgt catacttgtc ctcattaatt aggatatcca gtatttctct
     1021 tacttttcct tcaccttctc ctacatagcg gaactgattt tccaagcccg gttttgcgat
     1081 aagcgcatct ttgatgtgaa cataatcgat atgtccccta agtccttgaa aaaacttcat
     1141 aggcttatca taaccgtgag ccactacatt tcccgtgtcg taaaggagca caaagttggg
     1201 gctgtttact tcctgtttaa gctccagcat attttccgga gcttctcctc cccaacccgt
     1261 gcagttttca tgagccaata ctatatcaag atcctcggcc atctttgcaa gttctttaca
     1321 tcttctgatt acttctttct tcgtatatgt tttgtcatct tcatcgggta tccaggacat
     1381 tatccttata tacctgacat tgagcatctt catcctcttt gcagatattt tcagatcctc
     1441 tttgtcaata ttgaaatccc ctcttatgct cctgctccag ttacctatag ctgaagaaaa
     1501 tgcagttact ttaaagttgt gttcttcaat aattttcctt acattatcaa actcctgatc
     1561 actgaatttg gtggtggaag gattgacacc gttatgattt cttaattcca aggtattcca
     1621 ccctaatttt ttgtgcgctt caatctgcgt ggtaatatgt ttgcccgctt catcgctgat
     1681 tcccgtaata atcatttctc acttataatc caatcatcgg tgatggtgat tggattttcc
     1741 tttctcccgg tctcttccgg gtaattcctt accatctttg gtatatttag tttaggtgct
     1801 gtggatttgt atcttttcct ctacagcttt aactggtacg aggtgactca gtccacagct
     1861 tttcgtctat tactgttaat cagtttgtta acgcttgacg tctctattta tgtgattaca
     1921 cagctgaatt ctctgtggaa gacaacacct ctttgttatt attttctcgt atttcttcta
     1981 aaaccccgtc actccagagt ttctcgaggt tatacctgct tctggtttct ttttcaaata
     2041 tatttaccac tacatccata cagtctataa gtatccattt agcttggtta tacccttcca
     2101 caggcatgga aaccgtcatt cccaacgctt ccgccttctc ttgcacatca tcggccatgg
     2161 atttacgatg acggtcggaa gttcccgtac atattacaaa atagtctgtc agtgtagtta
     2221 catctcttac ctctatggat atcaaatcct ttgctttttt ttctttcagt acctcaacaa
     2281 tttcattctt aaaattttca gtatccaata ttacctcctt gtatgttttg attatatcat
     2341 aaagagtaag ttataaatgg aaaaatgaat attcggcatt attaagaata ttaaaactat
     2401 ccgaaaacat tgcatattat caataaaagc attattatgc atacaggata aaaccgggag
     2461 gaaagtacaa tcaacgaatt actgaaagta acattgatat ttataggaat atttataatt
     2521 cagtcgatgg tatactgggg cactcagttt cttgttaaaa aacctcatat gctgggaagt
     2581 aaactggatg ataagatacc gtttataccg gttttcattt tcccgtatgt tatctggttt
     2641 gttcttttgt tcacaataca gtttgttcta tacttttatt ccctgcaaac atatgctctg
     2701 tatgtaatgt ctttacttat agtggatata actagcggaa taatatatct ggtatatccc
     2761 acaactttca tcagacccga acccgaagga gattcttttt ccgttaaagt agtaagattc
     2821 atttactcgc atgataagaa aatactgagc tgtctgcctt ctttgcactg ttctctatcc
     2881 acgcttttta tattatcggc actttatgcg accggtcttc cgttattttt aaaactgata
     2941 attattatat tttcactact cgttatagta tcaacggtat ttgtaaaaca gcatgtaatt
     3001 atcgatgtta ttaccgccat acctctggcc gttataagct ggtatatagc aatggcaata
     3061 ggtgccgata catttcttac cttcttcaac tttatacagt gatttaattg ttcttttaca
     3121 tttccggtat aaaatatatt tatgagatag gaggtgttaa ttatgaaaac ggcaataata
     3181 tattactcat atggcggtaa aacaaaaaag tactgcgaaa atatggcagc ggaactttcc
     3241 gccgatatcc tggaagtgca gacagtcaaa agaaagtgtt ttttatcaaa tctttttacc
     3301 gaatgcccta aggcactaca acagcaacct acggatattt tgcctataga cttggatttg
     3361 tcgggttatg aaaaaattat actggcagcg cctatgtggg caggcttccc cgcaccccct
     3421 ttcaataaca tggtagccat gttacctaaa ggcaagcaag tggaaatata tattgtttcc
     3481 gcaagcggtg aaactgatcc gataaaaaaa gaaaaggtaa tagtactgac tgaagaaaca
     3541 ggttcaaaag tcattaagta tacagatata tgcggtgcgg atataaaata ggttattttg
     3601 tttcgtttaa aaaccgtact ttattaattt tagcgatatc cgatgcaata gtctcggctt
     3661 ccgttacggt atctttggga acgggtatcc ggcactggag cttttgaggt ccgaatcgct
     3721 gccatattgt aaaattaacc gttatggtat agttgtcttc acggtctttg gcaaactgta
     3781 cagatgtgat tcttgttaaa ggtgctttcc actgaaaata ctcacccata atccatgcac
     3841 cgaacggact tattaacact tccggagcag cctctttcat cttgttatac cgagccctgg
     3901 gtattaaaat agccgctaaa gatatcaata tctgaatacc cagcaatact acaatgaaga
     3961 atatggcatc cttactgttg ccgaaaccga atattgcaaa taatcctcct attatcacaa
     4021 caaacgcaat tattaccagc agcattactt tgttggaggt ttttctttgc aggaactctt
     4081 ccgccgctct ttgcagtctt tcattctctt caaatctcca atgggctaat aaacccgttc
     4141 ctttcagcat gctgtccaga tttctcgcct gccggataaa cataaaaaag catataaaga
     4201 aagtaaggaa taatatagct ccgacataaa tcatccagta gtaattcatc tcgcctataa
     4261 ccgctcccgc tacaatcatc gctattccga taagcataaa gattatagac ataatcaccc
     4321 atctctttgc attattctgt gcccacatat cttacctccc gattatttta agaagatttt
     4381 ccgcaatatg attaaatccc gaatctgcgg gatgaaccgt atggtcctga tgatatttgg
     4441 tgtcatgcgg cataagagcc aagccgttta cgggaataaa accgtatttc tttgctaatg
     4501 tcgttaattc tttttgatac tcatcaaaca tttccttaaa tgattggtct tcatcgtatc
     4561 ttaaacacca tatcggagta ataatatata cttttgcatg cttgtagata ttcttaagct
     4621 tgtataaata cttgtcggct gttttcagta cattttccag ttccgccata tcttttaatt
     4681 gatagatatc attgatgccg tatgcaataa aaatactgtc gggatcatat ttgtcctgtt
     4741 catccaaaga atccacatca aacttcgccg aaccgacacc cctgttcaga ctctgtgcat
     4801 tcaaggccct tgcaacctgt gtgggaaaag ccaacgaagg actcaaagct accatgccct
     4861 gggtaatgga atctcccaga aaaagaagtt tttgagaggg ttgctcttta atacattgaa
     4921 aattacctat atccaaatca tacaccctta ttctgctcag attaggtaca taaacggtca
     4981 ctctctttat acctttttcc gttaacggat aagttaaagt agactgcttt gtatccaatg
     5041 cttcttctat gtgtctttta aatataccgt tttcgtaaaa atcaaatccc acatatcttc
     5101 tggaaaaatg gtcggctctg taggaaaagg ttatacactc ggcatcggta taaaaatcca
     5161 tacatatacc ggctgtacac agcgaccggt cttccgttgc cttggaaagg tttttatagt
     5221 attccagctg cttatcggta aaccgaacgg gaaaatatcc ttcctcggtt tttataacct
     5281 tgtatgcgtt tttgaaaata cccgtataca ttaatcttta atcctttgct ctttgaaata
     5341 ctccaaagcg gcagctgtta cgggatagag aacttctcct ttggaaagta catgatatac
     5401 cgtcccgtca aggctcagtt ccaccgcctc atcaagttta ttgtacatta atttgcgtat
     5461 tttagtcacg tttttgtatg ttcttccttt ttccgcagca tcggcaataa atactatttt
     5521 ttcggtaagt cccatccccg cccttccgaa cgagtgataa taaatggcat taagaatctc
     5581 ttcatcctct atgttgtatt ctttttttgc tattaccgcg gcacagggac cgtgcagtat
     5641 attaccggta tgatattgct tgtcaataaa aataccggca gcttcgatta ttgctgccat
     5701 atcttctgtt ttcatgtctt tggcaatatc atgcaaaagt gccgccgcat atactttttc
     5761 gctgtccgcc cggtgtaatc ttgcaagatg atctgccaat tcgacaacat tctttacatg
     5821 ctcatatctt tcggggctat acctttttac tatatccagc cttgcctctt cacttatata
     5881 tcccattttc ttttatatac ctgtacactt ttttgtccag catccactct atcgagttgt
     5941 cctgctccgc tatcccttta cgtattgcgg tagaggatat ttccgatatg cttcttatgt
     6001 cgaatataat gtcggcaccg taatcttttt tgaatatttc aatctgttcg gttatctgct
     6061 cttcggtaaa gcccgatctt ccgaatacca aaaacgtgca gagtttgaac acttcctcat
     6121 attctctcca tgtcttaagc tgaaacaggg tgtccgctcc tatgatatat attatttcgt
     6181 caaaaaccgg atataactct ttgacctgtc tcaatgtatc tatcgtgtat gtggttgttg
     6241 ctcttttaag ttccatgtcc gaagctatga gtttttcata accgtccaat gccagacata
     6301 gcatcgtata tcggatattg tcatcggtta taccggcatt atctttatga ggcggctttg
     6361 tacagggtgt caagagcaca tactcacaat aatccagtgc gtatttcgcc atcaggatgt
     6421 gagccttata taccggatca aatgttgctc ctaagattcc cagtttcatt ttctgcctat
     6481 atctttcctg taatgcatgt ctttaaagca tactttattg atattattgt atactcttgc
     6541 gaccgcttgt tcaacagtat ctccttttgc agttaatgaa aggactcttc ctccgttggt
     6601 aacatattcc ccgtctttca tggctgttcc catatgataa acggttattc cctgcttctg
     6661 cacctgctcc aaaccggata tcgggtaacc tttggtatat tctttgggat atccgccgct
     6721 tgcaagaacc accgtggcac cggcactgtt ctcccattga atgtcagtct tgtctaacct
     6781 ttcatctgtt atcgcattga atatatcaag aagatcggtt ttcagtaacg gcagaagtac
     6841 ttgtgtttcg gggtctccga acctggc
//
LOCUS       DAOPWC010000026         6848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11075_length_6848_cov_3.335345, whole genome shotgun sequence.
ACCESSION   DAOPWC010000026 DAOPWC010000000
VERSION     DAOPWC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11075_length_6848_cov_3.335345"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PKJ65_01135"
     CDS             <1..1318
                     /locus_tag="PKJ65_01135"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNZ40472.1"
                     /translation="MSDAVKPLAQMEGFTKILTMFSNPILGILTGAIFTAIIQSSSAS
                     VGVLQALSTTGVVTFGSAVPIILGQNIGTCATALISSIGTNKNAKRAALIHLFFNVIG
                     TVLFASLFYGLNAIIDFSFFNSVLNPVGIAIFHTFFNVLTTLVLFPFTKLLVRLSETI
                     IKDKGTVVEKIVLDERFLNTPSIAVEQCKRYIVTMANVTKETILMAIPLVKHYDAKKA
                     QVIVDNEDLIDTYEDKLGSYLVKLSGRELNDKDSKEISKILHSISDFERISDHAVNIL
                     DVAKELYDKDITFSEQAYKELDVMASAVEEILDLTVDAFLNNDFEKASKVEPLEQVID
                     ELKIILKNRHIERLQGGGCTIELGFILSDLINNYERVSDHCSNLAVLTIQIAEGLFDT
                     HEYLQNVKQSDRTHYMKYYRKYHDKYDVARIVDNNIKNEDSVALPK"
     gene            1447..2115
                     /locus_tag="PKJ65_01140"
     CDS             1447..2115
                     /locus_tag="PKJ65_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ40473.1"
                     /translation="MMLNKILIVEDEPALRNLLKTCLVKEGYQVLEAKDGQEALDIFY
                     EENISLVILDIMLPKLDGFEVLRTLRLESDVPVLILTARNTEYDELQGLRLGADDYIT
                     KPFSPAVLVSRVKAILKRAGIFADNDITAKDILMKIKEHEVYVKGEKINLTPKEFDLL
                     QYLIQNKNIVHSREKLLSAVWGYDFFGDARTVDTHMKCLRTKLGDSADIIKTVRKYGY
                     KLEV"
     gene            2138..3646
                     /locus_tag="PKJ65_01145"
     CDS             2138..3646
                     /locus_tag="PKJ65_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ40474.1"
                     /translation="MLIILCILLINIIATVLFSNVVFERFYINAKYNELKDYAGKINK
                     YFAEMDISLPDFRANLINAFDEIERNNIEIIWFIYNPTINHADIGYATKITGRYLANY
                     PPDTKEVEEYISALIESGDLNSIENVYEYKTSDINAGKQNINLITLVFENHDVRSNNM
                     LQSYIVLSSPKEYIATTAKTVARFVLHVSLVTLLMGVIAVVIISAQITKPIVKMNKVA
                     DKIAKLDFSESCDVSGKDEIGALAGSINEMSAKLQDSIDYLQQRAQLLKKDLEKQEGS
                     NKLKKEFIASVSHDFKTPLSLIIAYSELLNDKYKDDEEAKNQLLIITDQAVKMNLMVN
                     QLLTLSQLESGTVKKEDSIFCLSEAVESVVKDLHIMIEQAQIKLTFNYQDDYIVKGDY
                     NKITHVITNLLENAIKYCDEKKIINININEIDGKIRTSIYNTHRGIPEQSLPNIFNSF
                     YKEDKSRGGSSKSYGLGLSIVKAVMEMHNGKYGAYNSNAGVVFWFELEKRDI"
     gene            3748..5013
                     /locus_tag="PKJ65_01150"
     CDS             3748..5013
                     /locus_tag="PKJ65_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ40475.1"
                     /translation="MNNEYKTSENNPTNLYYQYNNMHNNRQDKKTTSRKTLVTAVVLC
                     SIFSLLLGLAGGFMAGRLYQGVFEWRYEFTPQESSSVIERERPRDNNKTPIYSTSEIE
                     KQYASVAEIYADSVDAVVGIVVEVESGSGWSAFTQSGSGSGVIISEDGYIVTNNHVIE
                     NAKKITVYLNDEESYTAKLVGLDVQLDLALLKIDADGLDYMVCGDSDNVMVGEVVLVI
                     GNPLGYLNQSLTVGYISGLEREIVLGNEKMVLLQTDAAVNPGNSGGALINIKGELIGI
                     INAKSYGSEIDGIGFAIPINDIKDVLDDIVNFGYVKGRPFLGVGLMDVTSQSTLNWYN
                     MMYGMNLTNLGVNVTKVQEGSAADKAGMKMYDCITEFNGVSVQSASHLKELIDSCTVD
                     QAVEIVVARGNETLTLTAIIGEEIPETSR"
     gene            5022..5759
                     /gene="truA"
                     /locus_tag="PKJ65_01155"
     CDS             5022..5759
                     /gene="truA"
                     /locus_tag="PKJ65_01155"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ40476.1"
                     /translation="MKNILLTIEYCGKNYSGWQRQLNGVSVQEKIEDAIEKLTGQKIG
                     INGSGRTDAKVNALGQCANFKIDCTIPPSKFGMALNSMLPEDIRIVRSEEVDMDFHAR
                     YSATGKHYRYTILNRTAPSAIYKHTTYHMKHPLNVDKMIKAGSCFVGKQDFRGFMSAG
                     SKIKKTVRTIYSLDITKDGDLIIIDIKGDGFLYNMVRIIAGTLTEVGRGNIPVEEIKD
                     IIQSRDRSRCGPTLKASGLCLMEVYYD"
     gene            5890..6426
                     /gene="raiA"
                     /locus_tag="PKJ65_01160"
     CDS             5890..6426
                     /gene="raiA"
                     /locus_tag="PKJ65_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNZ40477.1"
                     /translation="MKVSIVGRRVDITEEIKDWTEKKFAKLDKFFNGNTEVTVVVEAL
                     KQNKHTKVEATIVWKGVIFRAELSGNDLLGQIDKSVDIIERQVRKNRTRLEKRFHGDV
                     PDNWQGITQSAEYSEAAYNVVRTKRFPVKPMSVDEAIMQMEMLNHEFFMFVNEETNEM
                     NVVYKRKDGDYGLLEPYR"
     gene            6585..>6848
                     /locus_tag="PKJ65_01165"
     CDS             6585..>6848
                     /locus_tag="PKJ65_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40478.1"
                     /translation="MQSERFDYALNMLLEGEDINLLKKHAERISEDYRDGTGPSKRIV
                     NDAKKAKLLIHGDFIIGLPGETDKTAKSTFNFINEIKPDILQVS"
BASE COUNT         2344 a         1135 c         1569 g         1800 t
ORIGIN      
        1 catgtccgat gctgtcaaac cgcttgccca gatggaaggg tttaccaaga tacttaccat
       61 gttttccaat cctatactgg gtatacttac aggagccata tttaccgcaa taatacaaag
      121 ttcttcggca tctgtcggtg ttttacaggc tttgtccact accggagttg taacattcgg
      181 aagtgccgtt cctatcatat tgggtcagaa tatagggaca tgtgctacgg ctttgatttc
      241 cagtatagga acaaacaaga acgcaaaaag agctgcgctg attcatctgt tttttaatgt
      301 tataggaacg gttctgtttg catctttgtt ctacggtctt aatgccatta ttgatttttc
      361 attttttaac tcggtattga atcccgtagg aattgcaatt ttccatacat tttttaatgt
      421 gttgaccaca ctggttcttt tcccttttac caagttgttg gtgcggttgt ccgaaaccat
      481 tattaaagat aagggcacgg tagtagaaaa aatagttctg gatgaacgct ttctgaatac
      541 tccttccata gccgttgagc agtgcaagag atatatagta acaatggcca atgttacaaa
      601 ggaaaccata cttatggcaa tacctctggt aaagcattat gatgcgaaga aagctcaggt
      661 tattgtggat aatgaggatc tcatagatac ctatgaggat aaattgggat cttatcttgt
      721 caaattaagc ggacgggaat tgaacgataa ggacagcaag gaaatatcca agattctgca
      781 cagtataagt gactttgaaa gaataagcga ccacgcggta aacatactgg atgtagccaa
      841 ggaactttat gataaagata ttaccttttc cgagcaggca tataaagagt tggatgttat
      901 ggcttcagct gtggaagaaa tactggatct taccgttgat gcattcctta ataatgattt
      961 tgagaaagca tccaaagtgg aacctctgga gcaggtgata gatgagttga aaattattct
     1021 taagaatcgc catatagaaa gattgcaggg cgggggctgt acaattgaat tgggatttat
     1081 actgtccgac ctcataaaca attatgaaag agtttccgac cactgctcca atcttgcggt
     1141 tcttacaata caaattgccg aaggcttatt cgatacacat gaatatctgc agaatgtaaa
     1201 acagtcggat cggacccact atatgaagta ttatcggaag tatcacgata aatatgatgt
     1261 ggccaggata gtggataata atattaaaaa tgaagattcg gttgcacttc ccaagtagaa
     1321 accgcaaatg cataagcatc tgacagtagc aatagccgat gctattgctg tgttatcagt
     1381 gataaaaaca cagttttatc acaaatcggt gctataatta attaaaagaa agcattccgg
     1441 aggtttttga tgcttaacaa gatattaata gtggaggatg aacccgcttt aagaaatctt
     1501 ctgaagacct gcctggttaa agaaggctat caggtgctgg aagcaaaaga cggacaggaa
     1561 gctctggaca tattctacga agaaaacata tcactggtta tactggatat aatgttgcca
     1621 aagctggacg gattcgaggt cttaagaacc ttaaggctgg aatcggatgt tcccgttttg
     1681 attcttaccg caagaaatac cgaatatgat gaactgcagg gcttaagact gggagcggac
     1741 gattatataa caaagccttt ttcgccggcc gtacttgtat caagggttaa ggcaatatta
     1801 aaaagagccg gaatatttgc cgataacgat attaccgcaa aagacatact tatgaaaata
     1861 aaagagcacg aagtttatgt caaaggagag aagattaatc ttacccccaa ggaattcgat
     1921 cttcttcaat accttataca aaacaaaaat attgtgcatt ccagagaaaa acttctgtcg
     1981 gcggtatggg gatatgattt tttcggagat gcaagaacgg tggatactca catgaaatgc
     2041 ttacgcacaa agctgggcga ctcggcggat atcataaaaa ccgtacgtaa atacggatat
     2101 aaactggagg tataaaatca aacttcgcaa taagcttatg ctcataatct tgtgtatact
     2161 tcttatcaat atcattgcta cggtattgtt cagcaatgtt gtatttgaac gtttctatat
     2221 aaatgccaag tataacgaac ttaaggatta tgcaggaaaa ataaataagt attttgcgga
     2281 aatggatatt tccctccccg atttcagggc taatcttatc aacgcttttg acgagattga
     2341 acgaaataat attgaaataa tctggtttat atataatccc acgattaatc atgcggatat
     2401 agggtacgct accaagatta ccggaaggta tcttgccaac tatcctcccg acaccaagga
     2461 agtggaggaa tatatttccg ctcttataga aagcggtgac ttaaactcca tagagaatgt
     2521 ttatgaatat aaaaccagtg atataaatgc gggtaaacag aatataaatc tcataacgct
     2581 ggtatttgaa aaccatgatg tgcggagcaa taatatgctg caatcataca tagtcttaag
     2641 ttctcctaag gagtatattg ccacaaccgc aaaaacggta gcacggtttg tgctacatgt
     2701 ttcactggtg acattgctta tgggggtaat agcggtggtt atcatatcgg cacagataac
     2761 aaaacccata gtcaaaatga ataaggtggc agataagatt gcaaagctgg atttttccga
     2821 aagttgcgat gtgtcgggaa aagatgaaat aggagccttg gcggggagca taaatgaaat
     2881 gagtgcaaag ctgcaggaca gcatagatta tttacagcaa cgggcacagc ttttaaagaa
     2941 agatctggaa aaacaggaag gctcaaataa attgaaaaag gaatttatag cgtcggtttc
     3001 tcatgatttc aaaacacctc tgtcgctgat aatagcatac agcgaattgt tgaatgataa
     3061 atacaaagac gatgaggagg caaaaaacca actgttgatt attaccgatc aggcggtaaa
     3121 aatgaatctg atggtcaatc agcttctgac gctgtcgcag ttagagagcg ggacggtaaa
     3181 aaaagaagac agtattttct gcttaagcga agctgtggag tcggtagtga aagatttgca
     3241 tatcatgatt gagcaggcac aaatcaaact gacattcaat tatcaggatg actacatagt
     3301 caaaggcgat tacaacaaaa taacgcatgt gataaccaat cttctggaaa atgcaattaa
     3361 atactgtgat gagaaaaaga ttattaacat taacataaac gagatagacg gtaagataag
     3421 gacaagcata tataacacgc accggggaat acccgaacag tcgttgccca atattttcaa
     3481 cagcttttac aaggaagaca aatccagagg aggcagcagt aaatcgtacg gattgggatt
     3541 atccatcgta aaggccgtta tggaaatgca taacggaaag tacggcgcgt acaacagcaa
     3601 tgccggagtg gtattctggt ttgaactgga aaagcgggat atataaccga gtcggtgtgt
     3661 tatcacatga ataatatgat attttcacag ttttatcaca agcgacctgt ataataggca
     3721 ttgttatcag catgaggagg aaaaaacatg aataacgagt ataaaaccag cgagaacaat
     3781 ccgacaaatt tgtattatca atataacaat atgcataata accgacagga caagaaaacg
     3841 acatcaagaa aaacattggt gaccgcagtg gtgttatgtt caatattttc actgctgtta
     3901 ggtcttgcgg gcggatttat ggcaggcaga ctatatcaag gtgtttttga atggagatat
     3961 gagtttacac cgcaggaatc ttcgtcggta atcgagcggg aacggccgag agataataat
     4021 aaaaccccta tttactccac ttcggaaata gaaaagcaat atgcttcggt ggcggagata
     4081 tatgccgatt ccgtagatgc cgtggtgggt atagtagtgg aagtggaatc cggttccgga
     4141 tggtccgctt tcacacaatc aggttcgggc tccggagtca taataagtga ggacgggtac
     4201 atagtaacca ataaccatgt aatagagaac gcaaagaaaa taacggtata tctcaatgac
     4261 gaagaaagct acaccgctaa acttgtagga ctggatgtgc agctggatct tgctctctta
     4321 aagatagatg cggacggtct tgactatatg gtgtgcggtg acagtgataa tgtaatggta
     4381 ggagaagtgg tactggtaat aggtaatcct ttgggatatc ttaatcagag cctgacggta
     4441 ggttacataa gcggactgga aagagagatt gttttaggca atgagaagat ggttcttctt
     4501 cagaccgatg ccgcagtcaa tcccggcaat tcaggcggag cacttataaa cataaaaggt
     4561 gaacttatag gaataatcaa tgccaaatcc tatggaagcg agattgacgg aataggtttt
     4621 gccataccca ttaacgatat caaggatgtt ctggacgata tagtcaattt cggatatgtc
     4681 aagggcagac cgtttctggg agtgggactt atggatgtaa cttcgcaaag tactcttaac
     4741 tggtacaata tgatgtacgg aatgaatctt accaatctgg gtgtaaatgt gaccaaggtt
     4801 caggaaggtt ccgcagctga taaagccggt atgaagatgt atgactgtat aaccgaattt
     4861 aacggagttt ccgtacagag tgcaagtcat ctgaaagaac ttatagacag ctgtactgta
     4921 gaccaagcgg tggaaatagt tgttgcaaga ggtaacgaaa cattaacatt aaccgcaata
     4981 ataggagagg aaataccgga aacatccaga tagaatatat gctgaaaaat atattattga
     5041 caatagaata ctgcggtaag aattattcgg gatggcaaag acagcttaac ggagtgtcgg
     5101 tgcaggagaa aatagaggat gccatagaaa agcttaccgg acaaaagatc ggtattaacg
     5161 gcagcggacg taccgatgca aaagtgaatg ctttaggtca gtgtgccaat tttaagatag
     5221 actgcacaat tcctccttct aaattcggta tggcactgaa cagtatgctg ccggaggata
     5281 taaggatagt caggtccgag gaagtggaca tggattttca tgcacgttac agtgccacgg
     5341 gaaaacacta cagatataca atactaaaca gaacggcgcc ttcggcaata tataaacaca
     5401 ctacatatca tatgaaacat cctctgaatg tggacaaaat gataaaagcg ggatcatgtt
     5461 ttgtaggcaa acaggatttc cgcggcttta tgtcggccgg aagtaagatt aaaaaaactg
     5521 taaggaccat atacagtctg gatataacca aagacggaga ccttatcatt atagatataa
     5581 aaggagacgg gttcctgtac aacatggtaa ggataatagc gggaacactg acggaagtgg
     5641 gcagaggcaa catacctgtt gaggagataa aagatattat acaaagccgg gatcgttccc
     5701 gatgcggacc gacattaaag gcaagcggct tatgccttat ggaagtttac tacgattaaa
     5761 ttatttgtta aaaatatttt aattatttat caaatatatg taattgattt attgtagccg
     5821 ggtttttcat gcggtataat ataagtatca atacacatat gaatacatac aacgaaagga
     5881 gacagtttta tgaaagtttc aattgtcgga agaagagtcg atattaccga ggaaataaaa
     5941 gactggacgg aaaagaaatt tgcaaaactg gacaagtttt ttaacggaaa caccgaggta
     6001 acggtagttg ttgaagcttt aaagcagaac aagcatacaa aggtggaggc caccatagta
     6061 tggaaaggag ttatcttcag agccgaactt tccggtaatg atctattggg acagattgat
     6121 aagtcggtag atattattga aagacaggta agaaagaaca gaacaagact ggaaaaaaga
     6181 tttcacgggg atgtgcccga taactggcag ggtatcacac agagtgcgga atattccgaa
     6241 gccgcatata acgtagtaag aacaaaaaga ttcccggtaa agcctatgtc ggttgacgag
     6301 gctataatgc agatggaaat gctcaatcat gagtttttca tgtttgtaaa tgaagaaacc
     6361 aatgagatga acgttgtata taaaaggaag gacggagatt acggtttatt ggaaccgtac
     6421 agataaacaa aaaaacaacc ggctgaaagg ccggtttttt tgaatataaa ggttataaag
     6481 cggatataaa atcggatatt taattttaaa gattgtagaa ccgtgttacg gatttagtcg
     6541 gtatatccgt tatgttatac taaacgggaa aggacagtat tttaatgcaa tcggaaagat
     6601 ttgactatgc cttgaatatg cttttggagg gtgaagatat aaatcttctg aaaaagcatg
     6661 ccgaacggat aagcgaagat tacagagacg gtacaggtcc gagtaagcgt atagtgaatg
     6721 atgcaaaaaa agctaaattg ctaattcatg gggattttat aataggatta cctggtgaga
     6781 cagataaaac tgcaaagagt acgtttaatt tcatcaatga aataaaacca gatatattac
     6841 aggtgtct
//
LOCUS       DAOPWC010000027         6745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11277_length_6745_cov_4.057997, whole genome shotgun sequence.
ACCESSION   DAOPWC010000027 DAOPWC010000000
VERSION     DAOPWC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11277_length_6745_cov_4.057997"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1041
                     /locus_tag="PKJ65_01170"
     CDS             13..1041
                     /locus_tag="PKJ65_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNZ40479.1"
                     /translation="MGKIVTFGEIMLRLKSPYGERLLQSDKLEATFGGGEANVAVSCA
                     NFGMDTSYVTVLPDNDIAKACVRQLRGFGVNTDDIYYRNGRMGIYFLEPGAVQRPSKV
                     IYDREYSAISLVKPGDIDWKKVFNGASWLHVTGITPALTQNSALVTLEAVTAAKQAGL
                     TVSIDLNYRGNLWKYGKKASEVMPSIVKFTDVCVANEEDVQKTLEIHNDADVTKGELD
                     DYKYKELSDKVLSTYPNIKVIAITLRESRNANINGWGACINDRKDFYVSRKYEITDIV
                     DRVGGGDSFAAGLIYGLNTYGDSKKALEFAVAASCLKHTVYGDFSRFTVAEVERLMKG
                     DGSGRVQR"
     gene            1038..2720
                     /locus_tag="PKJ65_01175"
     CDS             1038..2720
                     /locus_tag="PKJ65_01175"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNZ40480.1"
                     /translation="MTYKDKYNDWLKKADSDTIKELEAIKGNEKEIEDRFYKTLDFGT
                     AGLRGVMGAGTNRMNKYVIGQTTQALAQYVLEQGMEKRGAVIAYDCRIKSDEYAKQSA
                     TVLAANGIKVYLYESLRSTAQLSYSIRKLNAATGINITASHNPKQYNGYKVYWEEGSQ
                     ILDDIADAIAENISRIDPFNDVKTVDFNQAVKSGQITMLGKDMDDMFIEEVLSLTLND
                     DIDKDVKIVYTPLYGTGLVPVTRILRERGFHNVTVVEEQKEPDGTFPTTPYPNPENVK
                     VFEIPLKYGKKTDADMIIASDPDCDRIAMMIKNDNNEFVFCTGNQTGALLAEYMLSQR
                     KEKGLYSPKDAVVQSIVSGSVVPKIAKHYGTHFFETLTGFKYICGLANQFEKTGEYRY
                     VFGYEESIGYCIETFTRDKNGVSAAMMMCEMAGYYKKRGITLYEVLTEIYEKYGYMTE
                     NQISVEYPGIKGAKIKKHVMSQWRKKPLLKISGIKCSVCKDYINGYEGLPAQDALKYE
                     FEDGSWYALRPSGTEPKLKVYINAMGRSRKEADDLVKVFTEDISDYLAKCEK"
     gene            complement(2725..4188)
                     /gene="malQ"
                     /locus_tag="PKJ65_01180"
     CDS             complement(2725..4188)
                     /gene="malQ"
                     /locus_tag="PKJ65_01180"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ40481.1"
                     /translation="MRASGILLPVFSLPSPYGIGTMGKAAYEFVDFLVSAKQKYWQIL
                     PLGPTGYGDSPYQSFCSFASNSYFIDLDILESKDLLLKEEYQNEPWGENPDKVDYGLL
                     YRKRNKVLEKAFNRFDKENLSFKAFCRDNAYWINDYAIFMSLKEHFDGKEWQSWPEDI
                     RMRHNDALVRYGTELSEKILFHKFLQFEFFNQWTQLKKYANEKGIMIIGDIPIYVSPD
                     SSDVWSRHYLFLTDEDRNLTSVAGVPPDAFSATGQLWGNPLYDWDAMEQEDFYWWKQR
                     TGMASLLYDYVRIDHFIGIERYFSIPAGSDTAATGVYHQGPGMKLIDAINQSRGNMGI
                     IAEDLGVLTDNVRRLLEKTGYPGMKVLQFGFSGEPSNDNLPMHYKKNLIAYTGTHDND
                     TILGWYKKLDRKNKDFLRKYLNRSRVHVYDLIFETMKSVANTAVVPIQDWLCQGSKSR
                     INFPSVAFGNWTYRITDNMLTESLAQKISDMTALCAR"
     gene            complement(4396..4749)
                     /locus_tag="PKJ65_01185"
     CDS             complement(4396..4749)
                     /locus_tag="PKJ65_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40482.1"
                     /translation="MYVLFIVLNETCCLEDILAGFLREGIGGATIIDSQGMGRAIAHT
                     KNEDIPMFGTLRMMLGNTHPYSKTIFTVLNDEQMVEKAVSAVRETVKGCTCSGVGLMF
                     TVPIGKVYRMVEEDK"
     gene            complement(4754..6013)
                     /locus_tag="PKJ65_01190"
     CDS             complement(4754..6013)
                     /locus_tag="PKJ65_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ40483.1"
                     /translation="MNVLLKIAIVLVVGIIGGRVARLVKLPNVSGYLVFGLLLGPSFF
                     NFISPADVQSFNIINELALAAIAFSIGNEFVLKEMKKIGKSIFIITLMEVIGAVLLVF
                     CILFFVFRQPFAFSIVIASMSAATAPAATLLVMKQYNAKGPLTRTILPVVALDDVFGI
                     VVFGIALSVAKIVTYKQQLSVASMIFGPLIEIAGSVGIGFVLGLLLSWLSQKASGRDE
                     LQAIALAAIGVATGISLLLNMSPLLTCIVMGTVLVNIQQNSIRVFDSVNSFAAPIYIL
                     FFTLAGASLDLSILKTVGLMGIAYVLARGLGKIAGAWAGAAMVKAEPAVRKYLGLGLL
                     PQGGISIGLMVLVRQQLPDYAVAINTIIMFSVLIYETSGPIFAKIALKKAGEIGIEEK
                     VRKPRRKGKKKSKVSSQKSESCIIDKG"
     regulatory      complement(6102..6231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(6325..>6745)
                     /locus_tag="PKJ65_01195"
     CDS             complement(6325..>6745)
                     /locus_tag="PKJ65_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNZ40484.1"
                     /translation="GVAFFFIAQICYIVSFSILVPITWLDILLFIGLEGSILLVYRFM
                     GYDAKELKIPMLLYSSAISFMTVKAVSLAFAKPFGFYHGITVVLGAVLFLFSDWLIAR
                     GILTGGRTLRARLLNLVTYYLGQIMLAFSIFLMFMKY"
BASE COUNT         2155 a         1384 c         1425 g         1781 t
ORIGIN      
        1 aggaggagtt gaatgggtaa gatagttact ttcggagaaa taatgctgag actgaaatct
       61 ccttacggag agaggctttt acagtcggat aaactggaag ccactttcgg cggaggagaa
      121 gccaatgtag cagtttcctg tgccaatttc ggaatggata catcatatgt aacggttctg
      181 ccggataacg acatagcaaa agcatgcgtc aggcagttaa gaggattcgg agtaaataca
      241 gacgacattt attacagaaa cggacgtatg ggcatatact ttctggaacc cggagcggta
      301 cagcgaccct caaaagtaat atatgacaga gagtattccg ccatatccct ggtaaagccg
      361 ggagatattg attggaaaaa ggtatttaac ggggcttcat ggttacatgt tacaggtatt
      421 accccggcat tgacgcaaaa cagtgcgttg gttactttag aagcggtgac tgccgcaaag
      481 caggcgggac ttacggtatc catagacctt aattacagag gaaatctgtg gaaatacggt
      541 aaaaaggcat cggaggtaat gcccagcata gttaagttta cagatgtctg tgttgcaaat
      601 gaagaggatg tacaaaaaac cctggagata cacaacgatg ccgatgttac aaaaggcgag
      661 ttggatgatt acaaatataa ggagctttcc gataaagttc tgtcgaccta tcccaatata
      721 aaggtcattg ccattacctt gagagaaagc agaaatgcaa atataaacgg atggggagca
      781 tgtataaacg acagaaaaga tttttatgta agcagaaaat atgagattac ggatattgtg
      841 gacagagtag gaggaggaga ttcttttgct gcaggactga tatacggact taacacatat
      901 ggtgacagca aaaaagcact ggagtttgcg gttgccgcaa gttgcttaaa gcataccgtg
      961 tacggcgatt tttcacgatt cactgtggcg gaagtggaaa gacttatgaa aggagacggt
     1021 tcgggaaggg tacaaagatg acatacaagg ataagtataa tgattggtta aaaaaagccg
     1081 attccgatac tataaaggaa ttggaagcta ttaaaggaaa tgaaaaagaa atagaagaca
     1141 gattctataa aactctggat ttcggaactg cgggattgag aggcgtaatg ggtgccggaa
     1201 ccaaccgcat gaacaagtat gtaataggac agaccactca ggctctggct cagtatgttt
     1261 tagagcaggg catggagaaa agaggagcgg ttatagcgta tgattgcagg attaaatccg
     1321 atgaatatgc aaaacagtcg gcaacggtat tggcggcaaa cggaataaaa gtgtatctgt
     1381 acgagagtct gagatctaca gcgcaactgt cctattcgat aaggaaactg aatgccgcaa
     1441 ccggtataaa cataaccgcc agccataatc ccaagcagta taacggatat aaagtgtact
     1501 gggaagaagg atcacagata ttggacgaca tagctgatgc tatagccgaa aacatttccc
     1561 gaatagatcc ttttaacgat gttaaaaccg tggactttaa tcaggcggtt aaatcgggac
     1621 agataacaat gctcggtaag gacatggatg atatgttcat agaagaggtt ctgtccttaa
     1681 ctttgaatga cgatatagac aaggatgtta aaattgttta tacacctctg tacggtacgg
     1741 gattggttcc ggtaacaagg atactcagag aaagaggatt ccacaatgtg accgtagtgg
     1801 aagagcaaaa agaacccgac ggaacattcc ccaccactcc ttatcccaat ccggagaatg
     1861 ttaaggtttt tgaaatacct ctgaaatatg gcaaaaagac ggatgccgat atgataatag
     1921 catccgaccc cgattgtgac aggatagcca tgatgattaa gaatgacaac aatgagtttg
     1981 tcttctgcac aggcaaccag acgggtgcat tacttgcgga atacatgttg tcgcaaagaa
     2041 aggaaaaagg actgtattcg ccgaaagatg ctgttgtgca gtccatagta agcggctcgg
     2101 ttgtgcccaa aatagcaaag cattacggaa cacatttctt tgaaacgctc accggattca
     2161 aatatatatg cggtctggca aatcaatttg aaaagaccgg tgaatacaga tatgtattcg
     2221 gttatgaaga aagcatcgga tactgtatag aaacatttac gcgagataaa aacggagtca
     2281 gtgcggccat gatgatgtgt gaaatggcgg gatattacaa aaagcgcggt ataactcttt
     2341 atgaagtgct gaccgaaata tacgagaaat acggttatat gacggaaaac cagatatctg
     2401 tggaatatcc cggtattaaa ggggcaaaaa tcaaaaagca tgtaatgtcg caatggagga
     2461 agaaacctct tctgaaaatc agcggcatta aatgttcggt atgcaaggac tatatcaacg
     2521 gatatgaagg gttgcccgct caggatgccc ttaaatatga gtttgaagac ggttcctggt
     2581 atgctttaag gccttcgggt accgaaccga aactgaaagt atatataaat gcaatgggac
     2641 gctcaaggaa agaagccgac gacctggtga aagtattcac cgaggatata tcggactatt
     2701 tggcaaagtg cgagaaataa cggattatct ggcacagaga gcggtcatgt cggaaatctt
     2761 ttgagcaagc gattcggtaa gcatattatc ggtaattctg taagtccagt ttccgaaagc
     2821 tacggaaggg aaattaatac ggctttttga accctgacac agccagtcct gtatgggtac
     2881 aacagcggta tttgccacgg atttcattgt ttcaaaaatc aagtcgtaca catgaacacg
     2941 cgacctgttt aagtattttc ttaagaagtc tttgtttttt ctgtccagct ttttatacca
     3001 tccgagtatt gtatcgttat catgtgtgcc ggtatatgca ataagatttt tcttatagtg
     3061 catgggaaga ttgtcattgg aaggctctcc ggaaaaaccg aactgcagca ctttcattcc
     3121 gggataaccg gttttttcca gtaatcttcg cacattgtcc gtcaacactc ccaaatcctc
     3181 ggcaattatg cccatattac ctctggattg gtttatcgca tcaatcaatt tcattccggg
     3241 accttgatga tatactcccg ttgcagcggt atcggagccg gcggggatgg agaagtatcg
     3301 ctcaatccct ataaaatgat ctatgcgtac ataatcataa agtagagaag ccatacctgt
     3361 tctttgtttc caccagtaaa aatcctcctg ctccatggca tcccagtcat agagaggatt
     3421 gccccacaac tgacccgtcg cggaaaaagc atcgggagga actcccgcaa ccgatgtaag
     3481 atttctgtcc tcatcagtca ggaacagata atgtctggac cacacatcgg aactgtccgg
     3541 tgatacatat ataggtatat cgcctattat cattatgccc ttttcatttg catatttttt
     3601 taattgtgtc cactggttaa aaaactcaaa ctgtaagaac ttatgaaaca atatcttttc
     3661 cgaaagctca gttccgtatc tcaccaaagc atcgttatgc ctcatcctta tatcttccgg
     3721 ccaggactgc cattcttttc cgtcaaaatg ttctttcaac gacatgaata ttgcataatc
     3781 attaatccaa tatgcgttat cccggcaaaa ggctttaaat gagagatttt ctttatcaaa
     3841 cctgttaaaa gccttctcca acaccttatt tctcttccta tataacaagc cgtaatcgac
     3901 cttgtcggga ttttcgcccc atggctcgtt ctgatactct tctttaagca gcagatcttt
     3961 gctctccaaa atatccaaat ctataaagta actgttggat gcgaaactgc agaaagactg
     4021 atagggagaa tcaccgtaac cggtaggtcc caaaggaagt atctgccagt atttttgttt
     4081 tgccgaaacc agaaaatcta caaactcata tgccgcttta cccatggtcc ctataccgta
     4141 aggtgaagga agactgaaga cgggaagtaa tattccggat gctctcataa taaacctctt
     4201 aacaatgaat atatccgaga ttacttagtt tgtaaagtgt tctttatact atttaatgca
     4261 cagtaattta acttcaatct aagcattaag tcagttatat taaagattga acagcaagtt
     4321 ttgctaatag atttgtgata ttaacagcta aacttgttaa tacagccgtt ctatatttaa
     4381 cagttaattg atagattatt tatcttcctc aaccattctg taaactttac cgataggtac
     4441 ggtaaacatc aggcctacac cgctgcaggt acaacctttc actgtttcac gaacggcgga
     4501 tacggccttc tccaccatct gttcatcatt caggacggtg aaaattgttt tgctgtaagg
     4561 atgagtatta cccagcatca ttcttaatgt gccgaacata ggtatatcct catttttggt
     4621 atgagcaatt gctctgccca ttccctggct gtctataatt gtggcgccgc ctataccttc
     4681 tcttaaaaat cctgcaagga tatcttccaa gcaacaggtt tcatttaata ctatgaacaa
     4741 aacgtacata atctcacccc ttatctatta tacatgattc acttttttgt gaagatactt
     4801 tacttttctt tttgcctttt ctgcgaggct ttctgacctt ttcttcaatt cctatctctc
     4861 ccgccttctt aagcgctatc tttgcgaata tcggtccgct tgtttcatat atgagaacgg
     4921 agaacatgat aatggtattg atagctaccg cgtaatcggg aagttgctga cgcaccagta
     4981 ccataagtcc tatggatatg ccgccttgcg gaagcagtcc caaacccaga tattttctta
     5041 cagcaggttc ggctttcacc atggctgcac ctgcccaggc acccgctatt tttcctaaac
     5101 ctcttgccag aacataggca atacccatca gaccgaccgt tttaagtatc gaaagatcca
     5161 agcttgcacc ggcaagtgta aagaagagta tatatatcgg cgccgcaaag ctgtttaccg
     5221 aatcaaatac tcttatggag ttttgctgta tattaactaa aaccgtaccc ataactatac
     5281 aggtaagcag cggagacata ttcaaaagaa gagagattcc ggtagctaca cctatggctg
     5341 ccaatgctat tgcctgaagt tcgtctcttc ccgaggcttt ttgtgaaagc caggataata
     5401 ataatcccaa cacgaaaccg ataccgacag atcccgctat ttcaataaga ggaccgaata
     5461 tcatggaagc aaccgataac tgctgcttat atgtaacaat cttagcaacc gacaaggcaa
     5521 taccgaatac cactattccg aagacatcat caagagctac tacggggaga atggttcttg
     5581 tgagcggacc cttagcgtta tactgcttca ttaccaataa tgttgctgca ggagcggtag
     5641 cagccgacat ggaagcaatt actatactga atgcaaaagg ttgtctgaaa acaaagaata
     5701 aaatgcaaaa aaccaataac accgcaccta ttacttccat aagagtgatt atgaaaatgg
     5761 atttacctat ctttttcatt tctttcaaga caaattcatt tcctatactg aaagcaatag
     5821 ctgccaatgc cagttcgttt ataatgttga aggattgaac atccgcgggg ctaataaagt
     5881 taaagaatga aggacctaaa agcaacccga ataccagata gccggaaaca ttaggcaact
     5941 tgaccagtct ggctaccctt cctcctatta ttcctacgac aagaacaata gcaatcttca
     6001 gcaaaacatt catataagac ctcccacagt atatatttca tatgcagtcc atcatgtttt
     6061 ttggcaacaa cttaacaaag cgcataaaaa tcatcatgta ctacattgct tggtgcaatc
     6121 ctatgggatt agctgtcggg tttgggccac caagctgccc atcttatcca gattcacccc
     6181 ttttcggttg gttcccccac tatcaaaaca gatattcgga tatccataaa atagtaacat
     6241 agaaagcagg ttaagtcaat gaagagcacc gttgtgtgtc aacccttgaa aagataaata
     6301 aatatagtta tgaaagcaaa gaaatcagta cttcatgaac ataaggaata tactgaatgc
     6361 cagcattatc tgtcccaaat agtaggtaac aaggtttaag agacgagccc ttaatgtcct
     6421 acctccggtt aaaataccgc gtgctatcag ccagtcggaa aacaggaaaa gtaccgcacc
     6481 cagcacaacg gtgataccgt gataaaaacc gaaaggtttt gcaaatgcca gtgaaacggc
     6541 ttttaccgtc ataaaagata tagcgctgct gtacagcaac ataggtatct taagctcctt
     6601 ggcatcataa cccataaacc tgtaaacgag caggatagaa ccctcaagtc ctatgaaaag
     6661 cagaatatcc aaccaggtaa tcggaaccaa tatggaaaaa gagactatat agcagatttg
     6721 cgcaataaag aagaatgcca cacct
//
LOCUS       DAOPWC010000028         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11371_length_6698_cov_3.768478, whole genome shotgun sequence.
ACCESSION   DAOPWC010000028 DAOPWC010000000
VERSION     DAOPWC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_6698_cov_3.768478"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="scfB"
                     /locus_tag="PKJ65_01200"
     CDS             <1..1264
                     /gene="scfB"
                     /locus_tag="PKJ65_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HNZ40485.1"
                     /translation="DKSKEEIVEIIKDKYPDLTYNDICECISDIEQLVRDNKLFSEDV
                     YKDIPVNKISAGNIVKALCLHVSHACNLNCSYCFASQGNYKGKRALMSFEVGKQALDF
                     LIKNSGNVRNLEVDFFGGEPLMNLDVCKQLVQYARSIEKQHNKNFRFTLTTNGMLIDD
                     DVIKFANEEMDNVVLSLDGRKHVHDRLRKDINGNGSYDVIVPKFKKLVDNRTKGTYYI
                     RGTFTHHNTDFTNDILHMADLGFTELSMEPVVCSPDDEYALNEKDLPVLLEQYELLAQ
                     KMLEREKAGKGFTFYHYMIDLNHGPCIHKRLSGCGSGREYLAVTPWGELFPCHQFVSD
                     PRYSMGDVWKGVTNTGLRKKFELCNTYTKEECKNCWAKLYCSGGCAANAYHATGNING
                     VYEYGCILFKKRMECAIMLQIARADTAD"
     gene            complement(1410..2966)
                     /gene="rny"
                     /locus_tag="PKJ65_01205"
     CDS             complement(1410..2966)
                     /gene="rny"
                     /locus_tag="PKJ65_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNZ40486.1"
                     /translation="MNHREIIGLVIIALLTIFSIVMFFLGIRHRKKVAEKEIGSAESE
                     AKKLILQGEKSAEERKREILIEAKEEVHKSRVEMERDLKERRKEIQRMESRVIQREEA
                     LDKKLASVESKEEILNQKMSDIDSKQKEIEQIHQDKLTELERIAALTVEEAKTYLLKT
                     LEDDIKHEKVLLLKEYEAKTKEESDNLARNIISAAIQRCAADYVAENTVSVVELPNDE
                     MKGRIIGREGRNIRALETLTGIDLIIDDTPEAVVLSGFDPIRREIARIALEKLIVDGR
                     IHPARIEDTVNKAKKEIENTIKIEGDKVSFEAGVHNLHPEIIRLLGRMKYRTSYGQNV
                     LKHSLEVALLAGIMAAELGLDVTLAKRAGLLHDIGKSIDFETEGSHIDVGADIARKYN
                     EHPVVLDAIMSHHGDKEPESAIAVLVQAADAISAARPGARRETLEAYVKRIEKLEEIA
                     NSFEGVEKSFAVQAGREIRIMIKPEKMDDDDMVLICRDIVKRIENEMEYPGQIKVNVI
                     REVRVVDFAK"
     gene            3193..4188
                     /locus_tag="PKJ65_01210"
     CDS             3193..4188
                     /locus_tag="PKJ65_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40487.1"
                     /translation="MNIKYIKDAIAHKPTAKVPYLIQLTRKANEKYGEKLLQDYYSKD
                     AMSAYKKGIISGCDAIYLSVKNHAVGISAPWWTWHDYKDYYLTSDPPPYMPATKGTGS
                     YTDFEQYTTFLKENFGSYVTVFIWGSHFEKAYFARGIENFLADMAGEPEFARNLLDTI
                     IRKNMVMLENIVTNGNVDGILLGSDWGSQKALLMSPQIWREMIKPGEQKEYDLIRAYK
                     KDVWIHSCGNVRQIIPDLCEMGVNVLNPIQPECMDIFEIKETFGDKLAFWGGISTQHT
                     LPYGTVEQVKAEAERVITRMSTNGGYVAAPAQEIQEDVPYENITALIDVLRYYGA"
     gene            4283..4531
                     /locus_tag="PKJ65_01215"
     CDS             4283..4531
                     /locus_tag="PKJ65_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ40488.1"
                     /translation="MKSAGVVRKIDELGRIVVPIELRKSLNIGKREAVEISMEDDCIL
                     IRKFEPGCCFCGSTDALEVFHGNKVCNKCIKDLSSATE"
     gene            complement(4514..5353)
                     /gene="rsmI"
                     /locus_tag="PKJ65_01220"
     CDS             complement(4514..5353)
                     /gene="rsmI"
                     /locus_tag="PKJ65_01220"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ40489.1"
                     /translation="MLYLVATPIGNLSDISKRAIETLSNCDYIASEDTRHTLRLLNAL
                     GIKKKLVSYHQHNMESSGQSILNDVINGMDVCLVSDAGCPAISDPGNELVKLMHRHNQ
                     KVTVIPGPNAAISALMISPFDTSSFCFEGFPPVKISEKKRFFEKIREELRTCIVYEAP
                     HKLIKTIDVARQVMGGDRRVSLVKEITKIFETVVVDSLDGIAEYLEQNPPKGEYVLVF
                     EGNTDTNKETENPCPHSERDIVEKYEELIKNGLSRKDAMRKVAVLFNMSRNDVYSILF
                     GSR"
     gene            complement(5356..6087)
                     /locus_tag="PKJ65_01225"
     CDS             complement(5356..6087)
                     /locus_tag="PKJ65_01225"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HNZ40490.1"
                     /translation="MLHEDERLDDLQLEGLKLIQNKKQFCFGTDAVLLSSFANVRSTS
                     SVIDLGTGNGVIPLLLYGKYKPEHITGIEIQKDTYDLAVRNIQLNNLEDKITVINGDI
                     RNIREYFKAGSFDHVVTNPPYLKTGTGIISTADTMAYARHEILCTLEDILKATKYLLK
                     TKGVFTMVHRPQRLDEILLLMKTYAIEPKTIRFVHANVKSPAILVLIQGLKDMNPSIS
                     VEPPFIIHKPDGSYTEELNILYGRN"
     gene            complement(6186..>6698)
                     /locus_tag="PKJ65_01230"
     CDS             complement(6186..>6698)
                     /locus_tag="PKJ65_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNZ40491.1"
                     /translation="YIKGFNPSIRMVFALPLPVGVSSDSEYADIWFNTEYECQYALNK
                     FSQALPDGFVLLEAYDVEKSTLMEDVGSAIYGFRVNSSKNLKKSLDKLISLDSIEVEK
                     SNKKGLVDILPHLYKIDYYDENRFTVLCKAGAKGNLHPRLLLKALEKYTNTEIEEISI
                     NRNKLFLKVQ"
BASE COUNT         1944 a         1392 c         1283 g         2079 t
ORIGIN      
        1 agacaaatct aaagaagaga ttgttgaaat tataaaagac aaatatcccg atttgacata
       61 taacgatata tgtgagtgta tttcggacat agaacagttg gtgcgggata acaagctgtt
      121 cagcgaggat gtatataagg atattccggt taacaaaata tctgcaggta acatagttaa
      181 ggcattgtgc cttcatgttt cacatgcatg caacttaaat tgcagttatt gctttgcaag
      241 tcagggcaat tacaaaggta agcgtgcttt gatgagtttt gaagtgggca aacaggcttt
      301 ggactttctt atcaaaaact ccggcaatgt aagaaatctg gaagtggatt tcttcggcgg
      361 tgaaccttta atgaacctgg atgtatgtaa gcaacttgta cagtatgcaa gaagtattga
      421 aaagcaacat aacaagaact tccgctttac cctcaccaca aacggaatgc tcatagatga
      481 tgatgtaatc aagtttgcca atgaagaaat ggataatgtg gtactcagtc ttgacggaag
      541 aaagcatgta catgacagat tgcgtaaaga tattaacgga aacggcagtt atgatgttat
      601 agtgcccaag tttaaaaagc tggtagataa ccgcacaaaa ggaacctact acatacgggg
      661 aacattcaca catcacaaca ccgatttcac aaacgacata ttgcatatgg ccgatttggg
      721 atttaccgaa ctcagtatgg aaccggtagt atgtagcccc gatgatgaat atgcacttaa
      781 tgagaaggat ttacccgttc ttcttgaaca gtacgagcta ctggcccaaa aaatgctgga
      841 acgggaaaag gccggaaaag gattcacctt ctaccactat atgattgact taaatcacgg
      901 cccctgtata cacaaaagat tatccggatg cgggtccggc agagaatatc ttgcagtaac
      961 accgtgggga gagctgttcc cctgtcatca gtttgtgtcc gacccccgat acagcatggg
     1021 cgatgtatgg aaaggtgtga caaataccgg tttgcgaaag aagtttgagc tctgtaacac
     1081 atacacaaaa gaagaatgca aaaattgctg ggcaaagttg tactgctccg gaggatgtgc
     1141 cgcaaatgca tatcatgcaa ccggtaacat caacggagtc tacgaatacg gatgtattct
     1201 tttcaagaaa cgcatggaat gtgctatcat gctgcagata gcaagagccg atacagcgga
     1261 ttagaaatat taaagtataa tacgtaaaag taatattata aataatattc actcctgaag
     1321 ggtaaaactt tttatcaacc ggtaagtaac agatacacaa aaaaatctaa aaaaaaagca
     1381 tgtactcgtt tgatacatgc ttttattact tacttggcaa aatctacaac tcttacttcc
     1441 ctgatgacat tgacttttat ttgtccggga tattccattt cattttcaat tctcttaacg
     1501 atatctctgc atatgagcac catatcgtca tcatccatct tctcgggctt tatcataatt
     1561 cttatctctc ttccggcttg tacggcgaaa gatttttcta cgccctcgaa tgaattagca
     1621 atctcttcaa gtttttcaat tctctttaca taagcttcca atgtttctct tcttgctccc
     1681 ggtcttgctg ccgatatagc gtcggctgcc tgaaccagta ccgcaattgc actttccggt
     1741 tctttatcac cgtgatgcga cattattgcg tctaatacca cgggatgttc gttatacttc
     1801 ctggctatat ccgctccgac atcaatgtga gaaccttcgg tttcgaaatc tatggacttg
     1861 cctatatcat gtaaaagacc cgctcttttt gccagtgtga catccagacc cagttccgca
     1921 gccataatac ctgccagtaa cgctacttcc agtgaatgtt taagtacgtt ctgaccgtaa
     1981 ctggttctgt acttcatcct gcccaataac cttattatct cgggatgaag gttgtgaact
     2041 ccggcttcaa aagaaacttt atcgccttct atcttgatgg tattctctat ctccttttta
     2101 gccttgttta cggtgtcttc tatccttgca ggatgaattc ggccgtctac aataagcttt
     2161 tccaatgcaa ttctggcaat ttcccttctt atcggatcaa atcccgacag aacaactgcc
     2221 tccggagtat catcaattat caaatcaatt cccgtaagag tttccaaagc ccttatattt
     2281 cttccctctc tgcctatgat acgccccttc atctcatcat tgggtaattc cacaacagac
     2341 accgtattct ctgcgacata gtctgcggca cacctctgaa ttgctgcgga aataatattc
     2401 ctggcaagat tatccgattc ttccttggtc tttgcctcat attctttcag cagaaggact
     2461 ttctcgtgtt tgatatcatc ttcgagtgtt ttaagaagat atgttttggc ttcttcaacc
     2521 gtaagtgcgg ctattctttc cagttcggta agcttatcct gatggatttg ctcaatttcc
     2581 ttttgtttgg aatcaatatc cgacattttt tgattaagaa tttcttcttt gctttccacg
     2641 gaagcaagtt ttttgtccag ggcttcttct ctttggatta ctcggctctc catcctctga
     2701 atttcctttc ttctttcttt gagatccctt tccatttcca ctctgctctt gtggacttct
     2761 tctttggctt caatcaatat ctctctcttg cgttcctccg cacttttctc accctgtaag
     2821 ataagttttt tagcttcgga ttccgcactg cctatttcct tttcagcaac tttttttctg
     2881 tgtcgtattc ccaaaaaaaa cattactatt gaaaatattg taagaagagc gataatgacg
     2941 agacctatga tttctctgtg attcattccc ttttattacc tccttattcg gttttcaaag
     3001 ttcatatgta aaattaaaat aaataatcca tacaatttgt gattttatta tttttttgca
     3061 tatatgtcaa taaattttac gcttattaca gagtacatta ggaaaattta taatttgcaa
     3121 acatttaaag tgccgatatt tatcattatt tattccttat acgatggtat aatagcttca
     3181 cgggagacac ttatgaatat caaatacata aaagatgcta ttgcacataa acctactgct
     3241 aaagttccct atcttataca gcttacaaga aaagcaaacg agaaatacgg agaaaaactc
     3301 ttgcaggatt attacagcaa agatgcaatg agtgcctaca aaaaaggtat catatccgga
     3361 tgcgatgcaa tatatttatc cgttaaaaat catgcggtag gaataagtgc tccctggtgg
     3421 acatggcatg attacaagga ttattatctt acatccgatc ctccgccata tatgccggct
     3481 acaaaaggca cgggcagtta taccgacttt gaacaataca caactttctt aaaagaaaac
     3541 ttcggaagtt atgtaacggt ttttatctgg ggcagccatt ttgaaaaagc atattttgca
     3601 agaggtatag aaaacttcct tgccgatatg gcaggcgaac cggaatttgc acgcaatctt
     3661 ctggatacaa tcatacggaa aaacatggtt atgctggaaa atatcgtaac aaacgggaat
     3721 gtcgacggta ttcttctcgg cagcgattgg ggctctcaga aagcgttatt gatgtctccc
     3781 caaatctgga gagaaatgat taaaccgggc gagcaaaagg aatatgattt aatacgagca
     3841 tataaaaaag atgtctggat acattcctgc ggaaatgtcc gtcagataat tccggattta
     3901 tgtgagatgg gagtgaatgt actgaatccc atacagcccg agtgtatgga tatatttgaa
     3961 ataaaggaaa ctttcggaga taagctggca ttctggggcg gaatctccac acagcacact
     4021 cttccgtacg gaaccgtaga gcaggttaaa gccgaagcgg aaagagtcat tacccgcatg
     4081 agtacaaacg gaggatatgt tgcggcaccc gctcaggaaa tacaggaaga tgtcccgtat
     4141 gagaatataa ccgcgttgat tgatgtactg agatattacg gtgcatagcc ttaaaatctt
     4201 atcatcaacc tgtaagttat cattgcatta ttgtgaatta attggtagaa taactcgtga
     4261 tgaattttat tggaggatta atatgaagtc agccggagtt gtaagaaaaa tagatgaatt
     4321 gggaagaatc gtagttccta tagaattacg aaaatccctt aatataggta agagagaggc
     4381 agtggaaata tccatggaag atgattgtat acttataagg aaatttgaac cgggatgctg
     4441 cttctgcggt tctaccgatg ctcttgaggt atttcacggc aataaagtat gcaacaaatg
     4501 tattaaagat ctgtcatctg ctaccgaata atatcgaata aacatcattt cttgacatgt
     4561 taaacaaaac ggcaactttt cgcatggcat ctttacggga aaggccgttt tttataagtt
     4621 cctcatattt ttcgactata tcccgttcgg aatgagggca agggttctcc gtttccttgt
     4681 ttgtatcggt attaccctcg aaaaccagta catactcccc tttcggcggg ttctgctcca
     4741 gatattccgc tataccgtcc agactgtcca caacaaccgt ctcaaatatc tttgttattt
     4801 cttttaccag cgatactctt ctgtcaccgc ccataacctg tcttgcgaca tctattgttt
     4861 taataagttt atgaggtgct tcgtacacta tgcaagtacg caattcctca cgaatcttct
     4921 cgaaaaaccg tttcttttcc gatatcttta ccggaggaaa accttcgaaa caaaaagaag
     4981 aagtatcaaa aggcgatatc atcagggcac tgattgcggc attgggtcca ggtatgaccg
     5041 ttaccttctg gttgtgccgg tgcataagtt ttacaagctc gttaccagga tcggatattg
     5101 cgggacaacc ggcatcggaa accagacaga catccatccc gttaatcaca tcgttaagta
     5161 tactctgtcc gcttgactcc atattatgct gatggtaact tacgagtttc ttcttgatac
     5221 ccaaggcatt cagcagacgc aaagtgtgtc ttgtatcctc cgatgcgata tagtcgcaat
     5281 tggaaagagt ttctattgcc cttttggata tgtcggaaag gttgcctata ggtgtagcaa
     5341 caagataaag cactgttaat tcctcccgta caatatgtta agttcttcgg tataactgcc
     5401 gtccggttta tgtattataa acggaggctc cacgcttatt gacggattca tgtcttttaa
     5461 tccctgtatc agcaccagaa tagcgggcga tttgacattt gcatgcacaa atcttattgt
     5521 tttaggctca attgcataag ttttcatcaa taacaatatt tcgtccaacc tttggggtct
     5581 gtgaaccatt gtaaagacac ctttggtctt cagaagatac tttgtagcct tcagtatgtc
     5641 ctctaaggtg cacagtatct catgccgtgc atatgccatc gtgtcggctg tactaattat
     5701 acccgttccc gtcttgagat aaggaggatt tgtcacgaca tgatcaaaac tgccggcctt
     5761 gaagtactcc ctgatattac gtatatcgcc gtttataacg gttatcttat cctcaagatt
     5821 gttcagttga atatttctta cggcaagatc atatgtatcc ttctgtattt ctatgcccgt
     5881 aatatgttcc ggcttatact taccgtacag aagaagagga attacaccgt ttccagtacc
     5941 gaggtcgata acactcgatg tgcttctaac attggcaaat gaagacagca aaacagcgtc
     6001 ggttccaaag cagaactgtt ttttgttctg tataagtttt aaaccctcca gctgaagatc
     6061 atccagcctt tcatcttcat gtaacatatg tatatattat acatttcgga aagtgcttta
     6121 tacaaataaa actttcattt tttgtcgata tctgatgata tcctgacata tattaaaaaa
     6181 agttgttatt gaaccttcag aaacagtttg tttctgttaa tggatatctc ttcaatttcg
     6241 gtatttgtat acttttccag ggctttgagc agaagtctgg gatgaagatt gccttttgcg
     6301 ccggctttac ataacaccgt aaatctgttt tcatcgtagt aatctatttt gtaaagatga
     6361 ggtaatatat ctacaagacc ctttttattg gatttctcca cttcaatact gtccaaagaa
     6421 atgagtttat ccagtgattt tttcaagttt ttactgctgt tgactctgaa tccgtagatg
     6481 gccgacccca catcctccat caaagtggat ttctctacat cgtaagcttc cagtaaaaca
     6541 aatccgtccg gcagtgcctg agaaaactta ttcaaagcat actggcattc gtattcggta
     6601 ttaaaccata tatctgcata ttcactgtct gaggatacac cgaccggcaa aggtaatgca
     6661 aaaaccattc tgatactcgg gttgaatcct tttatgta
//
LOCUS       DAOPWC010000029         6602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11580_length_6602_cov_4.338170, whole genome shotgun sequence.
ACCESSION   DAOPWC010000029 DAOPWC010000000
VERSION     DAOPWC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11580_length_6602_cov_4.338170"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..461
                     /locus_tag="PKJ65_01235"
     CDS             201..461
                     /locus_tag="PKJ65_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40492.1"
                     /translation="MKRVKSACIFQTLVFVQKPEYGFSKEHALKINREEFEHYIAALE
                     CSKTRYLIDDTVEQEDGSIVVRIRKQYNDKTDVSEYFKKNGG"
     gene            463..705
                     /locus_tag="PKJ65_01240"
     CDS             463..705
                     /locus_tag="PKJ65_01240"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HNZ40493.1"
                     /translation="MKTVDRIRAEIVKLYRTDPNIHIDVSMNRPRVRISNQEARIKSV
                     YPHIFNIETQGKCYSVQYTDILMKSVRIIELEEHNV"
     gene            complement(1077..>1278)
                     /locus_tag="PKJ65_01245"
     CDS             complement(1077..>1278)
                     /locus_tag="PKJ65_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40494.1"
                     /translation="RTIRKYGQIGKIEDPIKVYINEEYTFLSYPVIHGNGQVVYYNIA
                     FNSKNEISGLFTTLILPDRNNN"
     assembly_gap    1279..1288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1289..1807)
                     /locus_tag="PKJ65_01250"
     CDS             complement(<1289..1807)
                     /locus_tag="PKJ65_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40495.1"
                     /translation="MKEFLSGFMKDFLSKVLPSSLMVGIMYAILTAGGYLRNVKIGDY
                     DTPKVIREGNLSMFKSAIITNSILLGILIILISITLIAILTLMFKNKEIDYRVKIGII
                     LAVFIIFIIPYFCRNTPYTENDVYDTIESKAEDYITDFTNKEYDKMVKNYILNRNMNK
                     FVSKNIYSQAMER"
     gene            complement(1884..2159)
                     /locus_tag="PKJ65_01255"
     CDS             complement(1884..2159)
                     /locus_tag="PKJ65_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNZ40496.1"
                     /translation="MALKSTNIKIEEDLKKQAQQLFSDLGMDMTTAVNIFLCQAVKEQ
                     AIPFRIGKPAPNAETPEAMAEVAEMKKFPDRYKGYTDVDGMMKELLE"
     gene            complement(2279..2932)
                     /locus_tag="PKJ65_01260"
     CDS             complement(2279..2932)
                     /locus_tag="PKJ65_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ40497.1"
                     /translation="MKKGLTELVFILDKSGSMSGLESDTIGGFNSMLAKQKKVDGECF
                     VTTVLFDNDYELIHDRIDIKAIAPITEKEYYVGGSTALIDAMGKTINKIDNVQKNTAE
                     EYRAEKVMFVIITDGQENSSKEYTADKVRALVEKQKEKNGWEFVFLGANIDAVETAER
                     YGISRNRAVDYLSDKKGTALNYRVMADAVTSFRKSGEVDDSCFEEIRKDVKKRGKKK"
     gene            complement(3022..4038)
                     /locus_tag="PKJ65_01265"
     CDS             complement(3022..4038)
                     /locus_tag="PKJ65_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNZ40498.1"
                     /translation="MPFNIIRQDITKMKVDAVVNAANTRLKMGGGVCGAIFRAAGAEA
                     LQAACDKLAPIETGKAVITPGFDLPARFIIHTAGPIYNMQTAKESERLLCSSYMESLR
                     VAIENRCESIAFPLISGGIYGYPKDRALQIATSAIRDFLKEQDIDIYLVLFDKSAFVI
                     GRELLGDVKSYIDEHYVERHEVIRERFLGAESRTMSKAKASIDGCFGPMYESADAAAT
                     LDDVIGNLDEPFSDMLLRLIDSKGMTDVEVYKRANLDRRLFSKIRSGKGYMPGKRTAI
                     AIAVALELSLEETEALLKRAGYALSHAVKFDVIVEYFIINGKYDIFQINEVLFEYDQP
                     LLGN"
     gene            complement(4204..4749)
                     /locus_tag="PKJ65_01270"
     CDS             complement(4204..4749)
                     /locus_tag="PKJ65_01270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ40499.1"
                     /translation="MNYIILDLEATCEQNNRDFPNETIEIGAVKIDENFNIVSTYNEF
                     IKPKINPILTDFCKQLTSITQEDIDNAKTFPDVIRHFKNWIGVGTEEYILCSWGFYDK
                     KQFTRDCELHGLDSSWTNRHISLKHQFGTIRNEKPCGMEKALNKLNIPLEGTHHRGID
                     DAKNIAKIFLMYKDKWVFTYE"
     gene            complement(4774..>6602)
                     /locus_tag="PKJ65_01275"
     CDS             complement(4774..>6602)
                     /locus_tag="PKJ65_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40500.1"
                     /translation="VLSFALIIVIIATALISASCNNPVFIRNSFNATTFNSDIVIKRT
                     DGQEPLNMPYSYSKALLDNRLLFEEEIIGLNISSVKYEFCDAGMNMYGNRDVFVNEDS
                     REVKAVMDSVKYCKGITTLSGIISDKEDSKITVYEGYSANLLTESLRNYVIIPSTLSR
                     HINKEIPDNEKVLFIVNPGTNGLAYFTIIGEYTTNNEKDTLYLSYEGFCNMVRGGQED
                     ISEHVDCMEIDVNEQADLKNFSFFLSAYFADINAPDQYENRINRLNEPYPYLFVNSVN
                     IKPISFTEDTNFKKNIITVSRIDKKENLLMSYLYSDALIKDYYEYSRYITDINISTGI
                     KGVNWRDYLTPADYNEYGLSVEPFDEFGNQWSDYRNKSGKNCADYFYQQAVTSISEIK
                     KMKSNCKITFYSNYTNRDLVVQRKEDYKEAAFMGEIMIAPPIKGYAIIPVTMYEAARN
                     RRYTDHHIIEMAEISAAYLARSALRVGFKVIGYYEVSEEQKVEVPDRKAEEIMVEFYD
                     HTGNLIKMPVVEKPEYISDGYDVIYLSYIGYNDRYEREQFRNEYIESITIETRSDADI
                     APLIRYLEQYFAPATDVKKYEKEKNVLGLDYEYCYTIKEKAD"
BASE COUNT         1897 a         1382 c         1089 g         2224 t
ORIGIN      
        1 aacaaatttt aaatttgtgc atgaaattta tttgaaaaaa tgctacaatt tttatgcaac
       61 acaaattgcc atgagagtga tttcagcaga gatttcgatg gcagtcggag tttcaactgt
      121 ttagtttcgc tcgaacacct ctctgcatta taggccacat atttctgccg taatgtatgg
      181 taccaaaagg aggtgttatc gtgaaacgag taaaatcggc gtgtattttt cagacactcg
      241 tttttgtcca gaagcctgaa tacggattca gcaaagagca tgcattaaaa atcaatcgcg
      301 aagaattcga gcattatata gctgctttag aatgttcaaa aacaagatat ttaattgatg
      361 atacggttga gcaggaagac ggttctattg ttgttcgcat aagaaagcag tacaacgaca
      421 aaaccgatgt ttccgaatac tttaaaaaaa acggaggcta atatgaaaac ggtagataga
      481 ataagagcag aaatagtcaa gctttaccgc accgatccga acattcatat cgatgtttct
      541 atgaaccgtc caagggtcag aatcagcaat caggaagcac ggataaaaag tgtttatccg
      601 catattttta atatagaaac acaaggaaaa tgctattcag tacaatacac ggatatttta
      661 atgaagagcg tcagaataat tgaacttgaa gagcataatg tataataatg agtgtttacc
      721 ggacactgtt ttttctatct ttatcttttc catgtcaata tatttccctt tcacttttgc
      781 tgtcatagtt ttcttttgcc ttattaaaca tcaataaatg tccgcccgga gtccatatat
      841 tgcctatact gaaagcacct accgttatgg tgttgaaaat aaaaaggcta ttgaatcagt
      901 caggcagacg ctgcaaagtt gaatataagt gaagaatctg ctgaagtgtt attaatagta
      961 ggaaaagagc agtaaatgaa gctggaactg cttaaatata taattcgtgt atgtttttta
     1021 atcatataat atatatgaca ttacgtaaga aatattatgt ttttaaaggt aacaaatcag
     1081 ttattatttc tatccgggag tataagagta gtaaataaac cggatatttc atttttactg
     1141 ttaaaggcaa tattataata tactacttga ccatttccat gtattacagg ataagacaaa
     1201 aaagtatatt cttcattaat atatacttta ataggatctt ctattttacc tatctgacca
     1261 tattttctta tagttcttnn nnnnnnnntc tttccatcgc ttgagaatat atgttcttgc
     1321 tgacaaattt attcatattc ctgttaagaa tataattttt aaccatttta tcatattctt
     1381 tattggtaaa atcggttatg taatcttctg ctttactttc aatagtgtca taaacatcat
     1441 tttctgtata cggcgtattc ctacagaaat atgggattat aaatatgata aaaacggcta
     1501 atataatacc aatctttact ctgtaatcta tttctttatt cttaaacata agcgtcaaaa
     1561 ttgcaatcaa tgtaattgaa attaagatta ttaaaatacc cagtaaaata ctattggtaa
     1621 tgattgctga tttaaacatg cttaaattac cttctcttat aactttcggc gtatcataat
     1681 caccaatttt tacatttctt aaatatccgc cagcggtaag tattgcatac attatgccaa
     1741 ccataagtga cgaaggcaga actttggaca aaaaatcttt cataaatccg gataaaaatt
     1801 ctttcataat aaactctcat tcttaaaata gtattctcaa taactattat tacacattat
     1861 tcattgtatg ttaactgcat atttcattca agcagctcct tcatcattcc gtcaacatcg
     1921 gtatatcctt tatacctgtc gggaaatttt ttcatttcag ctacctctgc catagcctcc
     1981 ggtgtttccg cattaggagc cggtttgccg attctgaacg gaatggcctg ctcctttaca
     2041 gcctgacata aaaatatatt gaccgctgtc gtcatatcca tacctaaatc ggaaaagagc
     2101 tgctgagctt gttttttcag atcttcttca atctttatat tggtgctttt aagtgccatc
     2161 ttgtcacccc tttcactact taaacatacg cctattgtat gtctgatatc aatacaatag
     2221 gcgcaacatt actggttata atcggtctgt atagcaacac tataaaacta ttcttttctt
     2281 atttcttctt tcctcttttt ttcacatcct tacgtatctc ctcaaagcaa ctgtcgtcaa
     2341 cctctcccga cttacggaac gaagtcacgg catccgccat aactctgtag tttagagcag
     2401 tacctttctt gtcggacaaa tagtctaccg ctctgttacg ggaaataccg tatctttcgg
     2461 ctgtctccac tgcatcgata ttggctccga ggaagacaaa ctcccatccg tttttctctt
     2521 tttgtttctc gaccaatgct cttaccttat cggcagtata ctctttactg gagttttcct
     2581 gtccgtccgt tatgataaca aacataacct tttcggcacg atactcttcg gctgtgtttt
     2641 tttgtacatt gtcaatcttg ttgatggtct tacccattgc gtcaataaga gccgtagagc
     2701 ctccgacata atactctttt tcggtaatag gggcgattgc cttgatgtct atgcggtcgt
     2761 gtatgagttc atagtcgtta tcgaataaga cagtggtaac aaagcactct ccgtctactt
     2821 ttttttgttt tgcaagcatg gaattgaaac cgcctatggt gtcgctttcc agtccgctca
     2881 tagaaccgct tttgtcgaga ataaatacca gttcagttaa tccttttttc atttcagtga
     2941 cctccttttt taataaactg ttcttacaat tcaatgatac taccttcttt aaaatactcg
     3001 gtcgcccggg aagcgacatt tttaatttcc taaaagcggt tgatcatatt caaacaatac
     3061 ctcgtttatc tggaaaatgt catacttacc gtttatgata aagtactcca cgataacatc
     3121 gaatttgacg gcatgggaca gtgcataacc cgcacgcttc aataaggcct cggtttcttc
     3181 aagtgacagt tccaaagcca ctgctatggc aatagcggtg cgcttgccgg gcatatagcc
     3241 cttaccgctt cttatcttcg agaacagtct tctgtccaga tttgcccgtt tgtaaacttc
     3301 aacatcggtc atgcctttgg agtcgataag acgaagaagc atatcggaaa aaggctcatc
     3361 aaggtttcct ataacatcgt ccagtgttgc ggctgcatca gcggattcat acatgggacc
     3421 aaaacagcca tcaatgcttg cttttgcttt gctcatagta cgactttccg cacctaaaaa
     3481 cctttctctt atcacctcgt gtctttcgac ataatgctcg tcaatatagc tcttgacatc
     3541 ccctaacagt tctctgccta taacaaatgc cgatttatca aacagcacaa gatatatatc
     3601 gatatcctgt tctttcagaa aatcccgtat tgcggaggtc gcaatctgaa gggcccggtc
     3661 tttgggatat ccgtaaatgc cgccggatat gagcggaaaa gcaatgcttt cgcatctgtt
     3721 ttctatcgct acccgtaacg attccatata tgaggaacat aatagtctct cgctttcctt
     3781 tgcggtctgc atgttgtata taggacctgc cgtatgaatg ataaacctgg cgggtaaatc
     3841 aaaaccggga gttataacgg cttttcccgt ttcaatagga gcaagcttgt cgcaagcagc
     3901 ctgaagtgct tctgctccgg cagccctgaa aatagcaccg caaacaccgc cgcccatttt
     3961 aagtcgggtg ttggccgcat tgactacggc atcaactttc attttagtta tgtcctgacg
     4021 aataatgtta aagggcataa atacttcacc tcttaaataa atcttatgtc atagtatctc
     4081 ttcttaatta tacaccaatc aacaggtata tatgctacgc tgtttttctt tcggactttt
     4141 gtttttcatc tgtgaaacat gtttcttagg acaagaaaca tgttatacgg ttatcctctg
     4201 tttctattcg taagtaaaaa cccacttatc cttatacata agaaatattt tagctatatt
     4261 ctttgcatca tcaatccctc tgtgatgtgt tccctccaaa gggatgttta acttgtttaa
     4321 tgccttttcc attccgcaag gcttctcgtt acgaatagta ccgaactgat gtttcaagct
     4381 tatatgcctg tttgtccagg acgaatccaa tccgtgcagt tcacaatctc tggtaaattg
     4441 ctttttgtca tagaaccccc aggaacaaag aatatattct tccgtcccca cacctatcca
     4501 attcttgaaa tggcggataa cgtcgggaaa agtttttgca ttgtctatat cttcctgtgt
     4561 gatacttgtt aactgcttac agaaatcagt cagaatagga ttgattttag gttttataaa
     4621 ctcattgtaa gtagaaacaa tgttaaagtt ctcatctatc ttaaccgctc cgatctcaat
     4681 agtttcattc ggaaaatctc tattgttttg ctcacaggta gcttctaaat ccaatataat
     4741 gtagttcatt tttacctcgc atattttcaa ctgttaatca gctttttcct ttattgtata
     4801 gcaatattcg taatccagtc ccagtacatt tttctctttc tcatatttct taacatcagt
     4861 cgcaggcgca aaatactgtt ccagatatct tataagcgga gcgatatcgg catcacttct
     4921 tgtctctata gttatagatt ctatgtactc gttcctgaac tgttccctct catacctgtc
     4981 attgtatcct atatatgaca gatatatcac atcatacccg tcagatatat actcgggttt
     5041 ctcaaccacg ggcatcttaa tcaaattacc ggtatggtca taaaactcaa ccattatctc
     5101 ctccgctttt ctgtccggca cctcaacctt ttgctcttcc gacacctcat aatatcctat
     5161 taccttaaat cctacacgca atgcactcct tgcaagatag gctgcactga tttcggccat
     5221 ttctataata tggtgatccg tatagcgacg attgcgagca gcttcataca ttgttacagg
     5281 tattattgca tatcctttta tcggaggggc tatcataatt tctcccataa atgcagcctc
     5341 tttatagtct tcttttctct gaacaacaag gtccctgttg gtatagttag agtagaatgt
     5401 tatcttgcag ttgcttttca tttttttaat ctcacttatg cttgtaacag cctgttgata
     5461 aaagtagtcg gcacaattct tgcccgactt gttacggtaa tcgctccatt ggttaccgaa
     5521 ttcatcaaaa ggttctaccg aaaggccata ttcattataa tctgcaggtg tcagataatc
     5581 ccgccaattc actcctttta tgcccgtact tatatttatg tcggttatgt accgtgaata
     5641 ttcataatag tctttaatca atgcatcgct gtataaatac gacatcagaa gattttcctt
     5701 cttgtcgatt ctggagacag ttattatgtt ctttttaaag tttgtgtctt ccgtaaaaga
     5761 tatcggcttt atattcacac tgtttacaaa caaatacgga taaggttcat taagtctgtt
     5821 tatcctgttc tcatactgat ccggcgcatt tatgtcggcg aaatatgcac ttaagaaaaa
     5881 tgagaaattt ttaaggtcag cctgttcgtt tacatcaatc tccatacaat caacatgctc
     5941 ggatatgtct tcctgccctc cgcgaaccat attacaaaat ccctcatagg acagatacaa
     6001 agtatccttc tcattatttg ttgtgtattc ccctattatg gtaaaatacg ccaaaccgtt
     6061 tgtaccggga tttactataa acagtacctt ctcattatca ggtatttctt tattgatatg
     6121 acggctcaat gtagagggaa taattacata gttccgtaag ctttcggtca gcagatttgc
     6181 ggaataacct tcataaacgg ttattttact gtcttccttg tcggatataa taccgctcaa
     6241 tgttgttata cctttacagt actttacgga atccataaca gcttttactt ctctgctgtc
     6301 ctcatttaca aaaacatctc tgttaccata catattcata ccggcatcac aaaactcata
     6361 cttaacggaa gatatattga ggccgataat ctcttcttca aataacaatc tgttatccag
     6421 caaagccttg gaataactgt acggcatatt caaaggctcc tgaccgtccg ttctttttat
     6481 gactatgtca ctgttgaagg ttgttgcgtt aaaggaattc cggataaata cagggttatt
     6541 gcaagatgcc gatatcagtg ccgttgcaat aataactatt attaatgcga aggataaaac
     6601 ag
//
LOCUS       DAOPWC010000030         6525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11751_length_6525_cov_3.313447, whole genome shotgun sequence.
ACCESSION   DAOPWC010000030 DAOPWC010000000
VERSION     DAOPWC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11751_length_6525_cov_3.313447"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PKJ65_01280"
     CDS             complement(<1..1861)
                     /locus_tag="PKJ65_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ40501.1"
                     /translation="MSRVCQNCRLRVSDKAKYCRKCGSPIGITNVPEYSAAENMMQFS
                     KPLEKSRNRLGLTGLKYMLIDLRKGLRRTTYLIPVFAVAVIWIAVSLLFSAGIDTVFL
                     RILSFFTYADAGMNGGFLGLTGGILGKMAYAYFIVMLLTPLYSLQKPFKGVIPGAKTY
                     ISQSFRFKTLKDVYVFILGMGFSFMAYNMLTGDNAWTKSIISLIIAVIFFRGMGKKYG
                     FTWDVFHRVFSDSRDEDTTLKAMGGYVTGLLLAVPTFLFNFPFLSYYIGIVLIVIFLV
                     LKFIEFIMLHKADFKRIFPIILVCMMLLYPLSALSAPVTSEIRGVWILQDVKLVNPDT
                     KKNPKDNTYVSDGYIRKQYSADSQYTIEYFWKSLPKKIHAGDESRVMIETRLVYTSAE
                     ETALKTQLNSAMFITYKPNPKEPEVNKYFPGSRDFSVEGVFTELQQEHTAFFLKITDY
                     YELYDLLTKNNCHLTVSVQASFGGRVEYIYRLQRTPVDGFLTWLAGDGTAYDHLGPLG
                     TVFSNVASVTASMLCAAVAVGSLTLKRKKGLLTDVKRTDKKEEIPEEKYTDIRTESDF
                     ADNLVNLYEIFSNAFAAGWPKYREVLTKTLSRSKDNAVAYKSVGAIESSINNYF"
     gene            1950..2474
                     /locus_tag="PKJ65_01285"
     CDS             1950..2474
                     /locus_tag="PKJ65_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNZ40502.1"
                     /translation="MMTRTKQIAYTGIFTAIIAVMSQLAIPMPSGVPATLQTLAIAIT
                     GLLLGIKAGTLSVSLYITIGTIGIPVFSGLRSGFGTLFGVTGGFIFGFIPFVLLCAVA
                     KNVKNRIAAIAICIAGLLTCHLCGIIQFMIITGKPFIQTALTVSLPYLLKDIVSCILA
                     YIISVKLSKVIAVE"
     gene            complement(2712..4673)
                     /locus_tag="PKJ65_01290"
     CDS             complement(2712..4673)
                     /locus_tag="PKJ65_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40503.1"
                     /translation="MKKRFIVQFVAIFVLLSVLVAPVFTGYAKGDTQDSQSEASSEVT
                     EISWDNRNTIHACVSVGVIRGEGGLIEDEYWLRNTTRAQGLLIGLRLRGLEDSARFYK
                     GTYSFNDMPGLNKAYWSPILAYAYEHPELGLVTDVKSGFRPSEVLTGNAFAKIIMEAL
                     GYKQGVDFESADVRSFAQSKGLTVKEGKVTNQDFTVVLQQALKLNNKEGKLFIEDLYQ
                     KGALYDRREVKAAPGTDPAIPYAARMRLFYYEGGWLQLHASDQYGQRILPPKGTFSSS
                     DTSKIVLGDQRPGVIATTDVSVDAYGVTSGINTAVVTFKSDDGKWNLSTEVTVVGKSD
                     VNVFTDIRYEQDYQALMVGKTMKLRKFSLINSLGNFVNLSFHQYYLEVRRADDNDPYG
                     TGDSAVFEIGRQEINEHYTYITAKKAGTETLKIVACKGGMPGSGNPNYIEAVKEFTLT
                     AVNDTDIVGCKVDFLYGKNFMYAGELSEDHNLKIKVSGATVNRMIVQIQSTDEVALPD
                     AFSYKVDVDNIAVSTEKSEIVTVRPVTEDMEAKIKVIGKRGYVIGVFPFQLKVDAPKV
                     HYLYMSAFNKDYVDVYAYDQYGKSIAVPKGTITSSDPDKIAITGTREGYNPYDLCYNI
                     SSVGDGPNTATITYVTDDGLFKAEVTVTR"
     gene            complement(4896..>6525)
                     /locus_tag="PKJ65_01295"
     CDS             complement(4896..>6525)
                     /locus_tag="PKJ65_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40504.1"
                     /translation="VQFVAIFVLLSVLVAPVFTGYANTDVKNTKAQTQNVKRPETDFI
                     LDFSKDKFDKVEAFVDGNYVDITDCWNSFQRFISASWFRYYTPEKRVLATEPRIDENS
                     KCIKFTKDNEVYYGTAYPLTHNAFGGYDGFHVWHDHVMYRYAVSNITLFHRVFDYIGY
                     WEINNWTNQKNTIIKASVYDMMCDSPLLIRKMNRSGDLYYWGQEKYAVVSAIANHYTK
                     TLEQKPDNLIKYGIRRHYTYVYSSITEIDVTLYAQSLWQKGLYAHIRMNDEEVWLVLD
                     TCVKDIQAELNNCDYYKVPALTKDMAALKLNGYDKVTLINNDKEYDITNMWFVNFDNK
                     GLCDWAEYAKAAPLKTDCKIILEKEGEKLVMNVARTYEYAVIEGKGLYVLPHIESIID
                     AVLQSDLHRAVDTLVKKSVLYKTYRSSFVFRRFFSEESKGYFYEETFQPANSLIIRYV
                     ETLPGKPDIETYKWVEIDFLYKADSIKMTVWRIDDYGDVGYVNLSGEGADIWYKIQDA
                     YMMFSYNGCDAVGDPWVRLNFLPEYTMVPDSMSK"
BASE COUNT         1900 a         1486 c         1082 g         2057 t
ORIGIN      
        1 tgaagtagtt gtttattgag gattctattg caccgacact tttgtatgca accgcattat
       61 ctttacttct tgaaagagtc tttgtaagta cctctctgta tttgggccat ccggctgcaa
      121 aagcattgga aaatatttca tagagattta caagattatc ggcaaaatca ctttctgtcc
      181 ggatgtcggt atacttctct tcaggtatct cctctttctt atccgttctc tttacatcgg
      241 tcagtaaacc tttctttctc ttcaacgtaa gtgatcctac cgctacggcg gcacacaaca
      301 tggatgcggt aaccgatgct acattggaaa aaaccgttcc taaaggccct aaatgatcat
      361 atgcggtccc gtcacccgcc aaccatgtaa gaaaaccgtc aacaggcgtt cgttgaagtc
      421 tgtatatata ctccaccctg cctccgaatg aagcctgtac cgaaacggtt aagtggcagt
      481 tgttcttggt cagcaagtca tagagttcgt aataatcggt gattttcagg aaaaaagcgg
      541 tatgctcctg ctgaagctcg gtaaaaactc cttcaacgga aaaatcacgg cttccgggaa
      601 aatacttatt aacttccggt tctttgggat tgggtttata cgttatgaac atagccgagt
      661 tcaactgtgt tttcagtgcc gtttcctcag ccgatgtata aaccagtctc gtttctatca
      721 tcactcttga ttcatcacct gcatgtattt ttttcggcag actcttccaa aagtattcaa
      781 tggtatattg tgaatccgcg gaatattgtt tccgtatata tccgtccgat acatatgtgt
      841 tgtctttagg attttttttg gtatcgggat taaccagctt gacatcctgc aatatccata
      901 ctcctcttat ctcacttgtt accggtgcgg ataaagcact taacggataa agcaacatca
      961 tacaaacaag gataatcggg aatatacgtt taaaatcggc tttgtgaagc ataataaatt
     1021 cgataaactt taaaacaaga aatatcacaa tcagcacaat accgatatag tatgacaaaa
     1081 acggaaaatt aaacaaaaag gtaggaacgg ccagtaaaag accggtaaca tatcccccca
     1141 ttgccttcaa tgttgtatcc tcgtctcttg aatcggaaaa taccctgtgg aaaacatccc
     1201 aagtaaaacc gtacttttta cccatacctc tgaaaaatat aaccgcaatt attaaggaaa
     1261 taatgctttt tgtccacgca ttgtcaccgg tcagcatatt gtatgccata aaggaaaaac
     1321 ccatgccgag aataaacaca tatacatcct tcagtgtttt aaaacgaaaa gactgagaga
     1381 tatatgtttt tgcccccgga attacaccct taaaaggttt ctgcaacgaa tacagaggtg
     1441 tcagcagcat tacaataaaa tatgcatatg ccattttgcc taaaatacct cctgtaagcc
     1501 ccaggaatcc tccgttcatc ccggcatcgg cataagtaaa aaaggaaagt atccttaaaa
     1561 aaaccgtatc aatccctgca gagaagagaa gacttaccgc aatccatatg actgctactg
     1621 caaatacagg gataagataa gtagttcttc tcaagccctt tcgtaaatcg ataagcatat
     1681 actttaagcc cgtaagcccc agcctgtttc tgcttttctc taaaggcttg ctgaactgca
     1741 tcatgttttc ggcagccgaa tactccggta cattggtaat acctatggga gatccgcatt
     1801 ttctgcaata tttggcttta tccgaaactc gcaacctgca gttttgacat actctggaca
     1861 attgcaccct ccctgtcaga aattgatttt atcataatat caggcatatt tacacactaa
     1921 gcaccccaag atagtgtata atgaataaaa tgatgacgcg aacaaaacaa atagcatata
     1981 cgggaatatt caccgcaata atagcggtta tgtcccaact ggcaatacct atgccttcag
     2041 gcgttccggc tacattgcag actctggcca tagctataac gggcttactg ttaggtatta
     2101 aggcgggcac cttgtccgta agcctgtaca tcacaatagg cacaataggt attccggtat
     2161 tctccggctt aagaagcggg ttcggtactt tattcggagt aacgggcggt ttcatattcg
     2221 gattcatacc ttttgtgttg ctttgtgcgg ttgcaaaaaa tgtaaaaaac agaatagctg
     2281 caatagcgat atgtattgcc ggtcttttaa cctgccactt atgcggcatt attcagttta
     2341 tgataataac gggtaaacct ttcatacaaa cggcacttac agtatcatta ccctacctgc
     2401 tgaaagacat agtatcctgt atactggctt acataatatc cgttaaacta agtaaggtta
     2461 tagctgtgga ataacagtta taacctctta ttttatagga atattaaata gtaatcactc
     2521 aatgcctgca aaacccaaga cattaaaact tataattgtt ccttcacaaa cctgctttgc
     2581 agataagttt ccttcggaat catagaactt tataacagac gggcaaggtc ataatgtgat
     2641 taccggaagc aacagtaaaa tacggacaaa acttttgtta ttccggattt cactcttttt
     2701 tccctaaaat attacctggt aaccgtcacc tcggctttaa acagtccgtc atcggtaaca
     2761 taagtaattg tcgcggtgtt cggaccgtct cctacagagg atatgttata acaaaggtca
     2821 tacggattat agccttctct tgtaccggta atagctattt tatccggatc ggaactggtt
     2881 atggttcctt ttggtaccgc tatactttta ccgtattggt cataagcata tacatccaca
     2941 taatccttgt taaaagcgga catatacagg taatgcactt tcggagcatc gacttttaat
     3001 tggaacggaa atacgcctat tacataaccg cgcttcccta taacctttat tttggcttcc
     3061 atatcttccg taaccggtct taccgtcaca atttcgcttt tttcagttga taccgcaatg
     3121 ttatccacat caaccttgta actaaaagca tccggtagcg ctacttcatc tgtagactgt
     3181 atctgaacaa tcatcctgtt tacagttgct ccgctgactt ttatcttcag attatggtct
     3241 tcggataatt ctcccgcata catgaaattc ttgccgtata agaaatctac cttgcatccg
     3301 acaatatccg tatcattgac tgctgtcaga gtaaactcct tgaccgcttc tatataattc
     3361 gggttaccgg agccgggcat accgcctttg caggcaacaa tcttaagtgt ctccgttccg
     3421 gctttttttg cggtaatata tgtatagtgc tcatttattt cctgtcttcc tatttcaaaa
     3481 accgccgaat cacccgttcc gtaagggtca ttgtcatccg cccttctcac ttcaagataa
     3541 tattgatgaa agctgagatt tacaaaattc cccagactgt ttatcaaaga aaatttccta
     3601 agtttcattg tttttcctac catcaatgct tgataatcct gctcgtatct gatatcggta
     3661 aaaacattca catcactttt acctaccaca gtcacttcgg tacttaaatt ccattttccg
     3721 tcatcggact tgaaggttac aactgcggta tttataccgg aagtaactcc gtatgcatca
     3781 acactgacat ccgttgtagc aataacaccc ggtctttggt cgcccaagac aatttttgat
     3841 gtgtccgaac tgctgaatgt gcctttcgga ggtaatattc tctgaccgta ttggtcgctg
     3901 gcgtgcaatt gtaaccatcc gccttcatag taaaataacc gcattctggc agcataaggt
     3961 atagcagggt cggttccggg agcagctttt acctctcttc ggtcatacag agcacctttt
     4021 tgatataaat cctctatgaa taatttccct tctttattat tcaacttcaa tgcttgctgt
     4081 aatactacgg taaaatcctg atttgtaact ttaccttctt ttacggttaa tccctttgat
     4141 tgagcaaagg atcttacatc ggcgctttca aaatccacgc cttgcttata acccaacgcc
     4201 tccataatta tctttgcaaa cgcatttccg gtcaaaacct ccgacggtcg gaaacctgac
     4261 ttgacatcag taacaaggcc taattccgga tgttcatatg cataagctaa aatagggctc
     4321 cagtatgctt tattcaagcc cggcatatcg ttaaaactat atgtaccttt atagaatcgc
     4381 gcactgtctt ccaatccgcg taaccgtaaa cctatcaaca atccctgtgc tcttgtggta
     4441 tttcttaacc aatactcatc ctcaataagg cctccttctc ctcttatgac tccgactgag
     4501 acacaggcat gtattgtgtt tctgttatcc caagagattt ccgttacctc cgaagaagct
     4561 tccgattgtg aatcctgtgt gtctcctttt gcataccccg taaacaccgg agcaactaaa
     4621 acagacagca atacaaatat tgctacaaac tgaacaatga acctcttttt cataccaaac
     4681 ctcgcattct ttgttaatta taaatattat atgttaacta aaagtaactt ctgtgttcca
     4741 ttatatacct ttaagattag caaattgctt tgttcataaa ttgtttacaa attcacggca
     4801 ttgtttacaa aaagttcata aattcctccg ttttgaatgt ttctttatct ttcttttctc
     4861 caatcttcca catgatttca tatatccgac cgtttctatt tactcatact gtccggaacc
     4921 atcgtatatt ccggaagaaa gttaagacgc acccacggat cacccacggc atcgcaaccg
     4981 ttatatgaaa acatcatata cgcgtcctgt atcttatacc atatatccgc accttctccg
     5041 gacagattta cataccctac atcaccgtaa tcatctattc tccaaacagt cattttgata
     5101 ctgtcagctt tgtaaagaaa atctatttct acccatttat atgtttctat gtcaggtttt
     5161 ccgggcagtg tttccacgta tcttattatc aaagagttag caggttgaaa ggtttcctcg
     5221 tagaaatagc ccttgctctc ctctgaaaaa aatctgcgaa atacaaagga agatctatat
     5281 gtcttataca aaacgctctt tttaaccaag gtatcaactg ctctgtgtaa atccgactgc
     5341 aaaacagcat caattattga ttcaatatgc ggtaaaacat acagtccttt gccttctatt
     5401 actgcatatt catatgttct tgcaacattc attacaagct tttcaccttc tttttcaaga
     5461 ataatcttac agtccgtttt taagggggcc gctttagcat attccgccca atcacaaaga
     5521 cccttattat cgaaattcac gaaccacata tttgtaatat catattcttt gtcgttgttg
     5581 attaatgtaa ccttatcgta tccgtttaat ttaagtgcag ccatatcttt tgtaagagca
     5641 ggcactttat agtaatcaca attattcaat tcagcctgta tatcctttac gcaagtgtct
     5701 aaaacaagcc acacttcctc atcgttcatt cttatatggg catataaacc tttctgccaa
     5761 agagattgtg cgtatagcgt tacatcaatt tcggttattg atgaatacac ataggtataa
     5821 tgacgcctta ttccgtactt aattaaatta tcgggtttct gttccaatgt ttttgtatag
     5881 tgattggcta tagcggacac aaccgcatat ttttcctgcc cccagtagta taagtcacct
     5941 gatctgttca tttttcttat gagcaacgga ctgtcacaca tcatatcata tacacttgct
     6001 ttaataatag tgttcttctg gttggtccaa ttgtttattt cccaataacc gatataatca
     6061 aaaactctgt gaaatagcgt aatattagat actgcatatc tatacataac atggtcatgc
     6121 caaacatgaa aaccatcata tccgccgaat gcattatggg ttaacggata cgccgtaccg
     6181 taatatacct cattatcttt tgtaaatttt atacatttac tgttttcatc tatcctcggc
     6241 tctgtggcta aaacacgctt ttcaggcgta taatacctga accatgaagc acttatgaac
     6301 ctttggaagg aattccagca gtcggtaata tcaacataat taccgtccac aaaagcctct
     6361 accttatcaa acttatcttt ggaaaaatct aaaataaagt ctgtctccgg tctttttaca
     6421 ttctgtgtct gagcttttgt attctttaca tcggtattag cataccctgt aaacaccgga
     6481 gcaactaaaa cagacagtaa tacaaatatt gctacaaact gaaca
//
LOCUS       DAOPWC010000031         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_11925_length_6453_cov_3.723351, whole genome shotgun sequence.
ACCESSION   DAOPWC010000031 DAOPWC010000000
VERSION     DAOPWC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11925_length_6453_cov_3.723351"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKJ65_01300"
     CDS             <1..581
                     /locus_tag="PKJ65_01300"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNZ40505.1"
                     /translation="SVVIKPEKDTITPRTVLLMPILAAGETAAAIEDIYSIKAGIKWP
                     NDILINGKKVCGILCESSVADNNVLLIVIGIGINVNQKAMHPDIADIATSLFMETAIQ
                     VPVDLLIQNICGNIACSYDKIRRGNTAGIIDKWNEYSIMNNAVITYTKGQSLYSAVSK
                     GIDRQGRLIAEQDGLTIYLDSSEISIKSTRSK"
     gene            581..898
                     /locus_tag="PKJ65_01305"
     CDS             581..898
                     /locus_tag="PKJ65_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40506.1"
                     /translation="MDDNRNTASANDGNRKRSNNNYYRKKKNYSGERKQDNLPAQATA
                     GEERDNRKSSQNKGYNNYKRYNRDRRRNRSGEETVDDIKKDIMRIEKEIRLEIEEIKM
                     IRV"
     gene            902..1708
                     /locus_tag="PKJ65_01310"
     CDS             902..1708
                     /locus_tag="PKJ65_01310"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ40507.1"
                     /translation="MLLAIDVGNTNIVMGVHNGEKYTNVWRLTTNKERTPEEFGVFYM
                     NMFNAAGLKLSDVDAIILSSVVPGIMYTLTSSLEKYFNRTPLLVEQGIKTGLNIKIDN
                     PRELGADIIVNLVSAYETYKGPAIVIDFGTATTFSCVTDNFECPGVVICSGVSLTAEA
                     LYKRAAKLPNVDIIKPAKVIGKNTITSMQSGIVNGYLGLIAGIVNDMKKELAEDYNQK
                     YEDIKVIATGGLLSIIAKNSDQIDYIDGMLTLNGLKLVYDKNKSKTEGKA"
     gene            1716..2351
                     /gene="tmk"
                     /locus_tag="PKJ65_01315"
     CDS             1716..2351
                     /gene="tmk"
                     /locus_tag="PKJ65_01315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ40508.1"
                     /translation="MAKGMIISFEGTDGSGKTTQIKALSRYLEEKGYMPAILREPGGT
                     RIGEEIRSILLNSDNTEMASLTEMLLYASSRAQMMEEKVEPLLKEGKIVILDRFFDSS
                     LAYQAYGRDLNFDAVLSINLAAVKNIVPKITFFIDITPEQSLKRRQNATATDRLENED
                     MSFHYKVYEGYKKLLERFPERIIRIDGTKEAREITNEITSYVDNVLKEDIR"
     gene            2348..2677
                     /locus_tag="PKJ65_01320"
     CDS             2348..2677
                     /locus_tag="PKJ65_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNZ40509.1"
                     /translation="MKLIFAIVNDEDELDVMRGLSQEGYGVTKLCSTGGFLKSGNTTL
                     LIGVDDEKLDHAIEIIKARSKSRKKIVNSSMIGASQGAVLTGYPIEITMGGATIFVTD
                     VVKYVKS"
     gene            2679..3632
                     /locus_tag="PKJ65_01325"
     CDS             2679..3632
                     /locus_tag="PKJ65_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HNZ40510.1"
                     /translation="MADFDDIKGRKPLKNQISKLMSKGRICHGYIFEGPVGSGKKLIA
                     DAFSRLLFCKNPDKLKVCGRCEGCLGHKEQYILKSDKSISVEDIRALIKDTQLLPLGS
                     GNKIYIIHDADTMTDQAQNAFLKALEEPPSNVYFLLTVTNSEQLIDTIKSRCVIIYTG
                     VNTESEVLDYLQSKYPDTDIQQINFAASFSEGCIGRADTLITDETFRQTRSAAVRYLS
                     EINTDLADKDKSTNITVFLEVLQSLFRDALIFKETEQTDNLINADFLDIIKRTAANYS
                     SESLFKACNYVAKAAQYIKDNVSASIAIGTMNVELMEELNI"
     gene            3629..4516
                     /locus_tag="PKJ65_01330"
     CDS             3629..4516
                     /locus_tag="PKJ65_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HNZ40511.1"
                     /translation="MITVVGVKFRHSNKIYSFAPGDWELNINDKVIVETIKGTEMGTV
                     ASPITQVEQSNVVAPLKEIIRKATQEDLNKAEESKSKESEAFAICEKKIADHKIAMKL
                     VGVEATFDMSKILFYFTADGRVDFRELVKDLASTFRTRIELRQIGVRDEAKLLGGIGI
                     CGRPFCCTSYLNEFQTVSIKMAKDQAISLNPLKISGVCGRLMCCLKYEHEVYTDLIKK
                     TPGIGAIVQTVDGKGEVIDVSMLKGELKVKLDKGEEIDIRQYNAQDVTVLKKSSKYKV
                     LPDEEAAQDINELTKLEDN"
     gene            4608..4778
                     /locus_tag="PKJ65_01335"
     CDS             4608..4778
                     /locus_tag="PKJ65_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ40512.1"
                     /translation="MPYRITDECISCGACEAECPVEAITQGDDIYVIDPELCISCGAC
                     ADVCPVDAPVEE"
     gene            4884..5477
                     /locus_tag="PKJ65_01340"
     CDS             4884..5477
                     /locus_tag="PKJ65_01340"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ40513.1"
                     /translation="MFEYKARINEVEFRFYSDSGVFSKTGIDYGSKLLIDSVIADIGE
                     QEKGLSNEERPSLADLGCGIGVIGIVLNRLLKINNTYLYEINERAAELAKKNAGQNRS
                     KRTYVNKLDIAKEDIPEKVDICVTNPPIRAGKATVFAFYEKAYKMLNENGIFYCVIQK
                     KQGLDSTKKKLTELFSNLETVNRSAGYHILKCIKDKN"
     gene            5580..6338
                     /locus_tag="PKJ65_01345"
     CDS             5580..6338
                     /locus_tag="PKJ65_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40514.1"
                     /translation="MGITHIIDDGLLISENRVIAGKSAKKEATKIASIKRAIFDDNDH
                     RKEVIKAIRAGKVDSLLILGTSDKMVNEIAQRLEVSPIQKIIRITDISDEEEIAAATR
                     SRQEKGKHVIPVPTFEIKKDFAGYLLDPLYIFRRRDKTRYEPDGTKTIVRPTFSYLGG
                     YTISNNTLFQIASYGCTEINGYEGMTRFRVINRPEGISLEVNITVNVDYDIPETLRQV
                     QQKIYDVLDFTTALNMIRIDVTAKNIMINGEVYP"
BASE COUNT         2224 a         1146 c         1515 g         1568 t
ORIGIN      
        1 tttccgtggt tatcaaacct gaaaaagaca ctattacacc ccgaacggta ctgttgatgc
       61 ccattcttgc ggcaggcgaa acggctgctg caatagaaga tatatacagt ataaaagcgg
      121 ggataaaatg gcctaacgat atattgatta acggtaaaaa ggtatgcggg atactgtgtg
      181 aaagcagtgt tgccgacaac aatgttcttc tgattgttat aggcataggc ataaatgtca
      241 atcaaaaagc gatgcatccc gatatagcgg atattgccac atcactgttt atggaaaccg
      301 ctatccaagt tcctgtcgac ctgctgatac aaaatatatg tggaaatata gcctgctctt
      361 atgataaaat tagacgcggg aatacagccg gcataataga taagtggaat gaatactcta
      421 taatgaataa tgctgtcata acctatacga aaggacaatc gttgtacagc gcagtttcca
      481 aaggaattga caggcagggc aggctgatag ccgaacagga cggattgacg atatatcttg
      541 actcgagcga gataagtata aaaagcacaa ggagcaaata atggacgaca atcggaatac
      601 cgcttccgca aatgacggca accggaagag gtcgaacaac aattattaca gaaagaaaaa
      661 gaactattcg ggtgagcgaa aacaggacaa tttgcccgca caggcgactg caggtgagga
      721 aagagataac agaaaatctt ctcaaaataa aggatataat aattacaaac gctataacag
      781 ggacagaaga agaaaccgca gcggggaaga aaccgttgat gatataaaaa aggatataat
      841 gaggatagaa aaagaaatcc ggctggagat tgaagaaata aaaatgataa gggtgtagag
      901 aatgctttta gctattgatg tgggaaatac caatatcgta atgggggtac ataacgggga
      961 aaaatatacc aatgtgtggc gcctgacgac caacaaagaa agaacccctg aagaattcgg
     1021 agttttctat atgaacatgt tcaatgcagc cggtctgaaa ctgtccgatg ttgatgccat
     1081 tattctttct tcggttgtac cgggaataat gtatacgctt accagcagtc tggaaaaata
     1141 ctttaaccgg acacccttgc tggtggaaca gggaataaag accggattga atataaagat
     1201 agacaatccc agagaactgg gggcggacat aatagtaaac ttggtctccg cttatgaaac
     1261 atataaaggt ccggctatag taattgattt cggtactgca accacattca gttgcgtaac
     1321 ggataatttc gagtgtcccg gcgtggtaat atgctcgggc gtcagtctta cagccgaggc
     1381 tttgtacaaa agagcggcaa aactgcctaa tgtagatata ataaaacctg ctaaggttat
     1441 aggcaaaaat actataacct ccatgcagtc gggtatagta aacggctacc tggggctgat
     1501 agcgggcata gtgaatgata tgaaaaaaga gcttgcggaa gactataacc agaagtatga
     1561 ggacataaag gttatagcca ccggaggtct tttaagcatt attgccaaaa acagcgacca
     1621 gattgactat atagacggaa tgcttacact gaacgggctt aaactggttt atgataaaaa
     1681 caaatccaag acggagggaa aggcataata cgtttatggc aaaaggaatg attatttctt
     1741 tcgaaggcac cgacggttcc ggaaagacca ctcagataaa ggctttgagc cgatatcttg
     1801 aagaaaaggg atatatgccg gcaattttac gggaaccggg aggaacaagg ataggagagg
     1861 aaattcgctc catattactt aacagcgata ataccgaaat ggcttccctg acggaaatgc
     1921 ttttatacgc atcttcccgg gcacagatga tggaagaaaa agtcgaaccg ttgcttaaag
     1981 agggaaagat agtgatactg gataggtttt tcgattcctc gcttgcatac caagcctatg
     2041 gaagagactt gaatttcgat gcggtactgt ccataaacct tgcagcggta aagaatatag
     2101 ttcccaagat tactttcttt attgatataa cacccgagca gtcgctgaag agaaggcaga
     2161 atgctaccgc aaccgaccgg ttggaaaatg aggatatgtc ttttcattac aaagtgtatg
     2221 aaggatacaa aaagcttttg gagagatttc ccgaaagaat aataaggatt gacggcacca
     2281 aggaagcaag agaaataact aacgagataa catcatatgt agataatgtt ttgaaggagg
     2341 atattagatg aagctgatat ttgcaatagt gaatgatgaa gatgaattgg atgtaatgag
     2401 aggactgagc caggaaggtt acggagtcac aaagctgtgt tccaccggag gattcttaaa
     2461 atcgggaaat accaccttgc ttataggagt ggacgatgaa aaactggacc acgccattga
     2521 aataataaaa gcgagatcca agagcagaaa gaagatagtt aattcttcca tgataggagc
     2581 atctcagggt gcggttctga caggatatcc gatagagata accatgggcg gagcaacgat
     2641 tttcgtaacc gatgttgtaa aatacgtcaa gagttaaaat ggcggatttt gatgatataa
     2701 agggaagaaa acctttaaaa aatcaaatat caaaacttat gtcaaaaggc aggatatgtc
     2761 acgggtatat atttgagggt cctgtcgggt cgggaaaaaa acttattgcg gatgcttttt
     2821 cccggcttct tttttgcaag aatcccgata aactgaaagt atgcggccgc tgtgaaggct
     2881 gtttggggca caaagagcag tatatactga aaagtgataa atccatttcc gtggaggata
     2941 tacgcgctct tatcaaagat actcaacttc tgcctttggg cagcggaaat aaaatctaca
     3001 ttatacatga tgccgataca atgaccgacc aggcacaaaa cgctttttta aaagcactgg
     3061 aagagcctcc ttccaatgtc tattttctgt tgacggttac caattccgaa cagctgatag
     3121 atacaataaa atctcgatgt gtaattattt ataccggagt caacaccgag agtgaggttt
     3181 tggattatct tcaaagcaaa tatcccgata ccgatataca acagataaac tttgctgcaa
     3241 gtttttccga gggttgcata ggaagagcgg acacacttat aaccgatgaa actttccggc
     3301 agaccagaag tgccgcggta agatatctca gtgaaataaa caccgattta gcagacaaag
     3361 acaaatcgac aaatataacg gtttttctgg aggtattgca aagcttattc cgtgacgcac
     3421 ttatattcaa agaaaccgag caaacagaca acttgataaa tgccgatttt ttggatatta
     3481 taaaaagaac ggcggcaaac tacagcagcg aaagcctgtt taaggcatgc aattatgtgg
     3541 caaaggccgc acaatacata aaagataatg tttccgcaag catagctata ggtaccatga
     3601 atgtggaatt aatggaggaa ttgaatatat gataaccgtt gtcggagtaa agttcagaca
     3661 ttcgaacaaa atatacagtt tcgctccggg agattgggag cttaatataa atgacaaagt
     3721 aatagtggag acaatcaagg gtacggaaat gggaactgtt gcatcaccga ttactcaggt
     3781 tgagcaatcg aatgttgtgg cccctcttaa ggagattatc cgaaaagcaa cacaggagga
     3841 tcttaacaaa gcggaggagt caaaaagcaa agaaagcgaa gcctttgcca tctgcgagaa
     3901 aaagatagcc gaccataaga tagccatgaa gcttgtcggg gtggaagcaa catttgatat
     3961 gagtaaaata ctcttttatt ttacagcaga cggaagggtg gacttcaggg aactggttaa
     4021 ggatctggca tccacttttc gtaccagaat cgagttaaga cagataggcg tacgcgatga
     4081 agccaaactc ttaggcggaa taggaatatg cggtcggccc ttctgctgta ccagttactt
     4141 aaacgaattt cagacagttt ccataaaaat ggcaaaagac caggctattt cccttaatcc
     4201 tcttaagata tcgggagtgt gcggaaggct tatgtgttgc ctgaaatatg aacatgaagt
     4261 atataccgac cttataaaaa agacaccggg tataggtgcc atagtacaga cggtcgacgg
     4321 caagggggaa gttatcgacg ttagtatgct taagggcgaa ctgaaggtta aattggacaa
     4381 aggcgaagag atagacataa ggcagtacaa cgcacaggat gtaaccgtac tgaagaagtc
     4441 cagcaagtat aaggttctgc ccgatgaaga agcggcacag gatattaatg aacttactaa
     4501 gctggaggat aattgatatt ttgataaaga tatatcaatt attcatatga ccttgatttt
     4561 tatatttcac ggctatataa ttgctacaag atacaaggag gtgtaacatg ccatacagaa
     4621 ttactgatga atgcatcagc tgcggagctt gcgaagctga atgtcccgta gaagctatta
     4681 ctcagggaga cgacatttat gttatagatc cggaattatg catttcatgc ggtgcatgtg
     4741 ctgatgtatg ccctgtagat gctccggtgg aggaataaca ggagtcaaat actgagaacc
     4801 ggcggccaga ccgccctttt tcattttaag gaaagataat gacaaatcac tactatacgg
     4861 acaaacagtt aactcatgag gacatgtttg agtacaaggc cagaataaat gaggtggaat
     4921 ttcgatttta ctccgattcc ggtgtatttt ccaaaaccgg cattgattac ggctccaagc
     4981 ttcttattga ctcggttatt gccgatatcg gggagcagga aaaaggcctt tccaatgaag
     5041 agagaccttc gcttgccgat ttaggttgcg gaataggggt tatagggata gtactcaaca
     5101 gactgcttaa gataaacaat acgtatcttt atgagataaa cgaaagagcg gccgagttgg
     5161 caaaaaaaaa tgccgggcag aacaggtcca aaagaacata tgtaaataaa ctggatatag
     5221 caaaagagga tataccggag aaagtggaca tttgtgtgac caatcccccc ataagagcgg
     5281 gtaaagcgac ggtgtttgct ttttatgaaa aagcatataa aatgcttaac gaaaacggta
     5341 tcttttactg tgttattcag aagaaacaag ggctggacag cacaaaaaag aaacttaccg
     5401 agctattttc caatcttgaa actgttaaca gaagtgccgg atatcatatt cttaaatgca
     5461 ttaaagataa aaactgatat aatcttaata tatatacgga ggtacacatt aaagtatatg
     5521 ccttagtcgg tccgagcgga accggcaaaa gtcataaatc cacgctgctt gcttcggata
     5581 tgggtatcac ccatataatt gacgacggct tattgataag tgaaaaccgg gtcatagccg
     5641 gaaaaagcgc caagaaggaa gctaccaaaa tagcttccat aaagcgtgcc atttttgacg
     5701 ataacgatca tcgcaaggag gttataaaag cgataagagc aggtaaagtg gactctttac
     5761 tgattttagg aacatcggat aagatggtca acgagatagc acagagactg gaagtgtcac
     5821 ctatacagaa aataataaga attaccgata tatccgacga agaagaaatt gccgccgcga
     5881 ccagaagccg tcaggagaag ggaaaacacg taatacccgt tcccactttt gagataaaga
     5941 aggattttgc cgggtatctt ttagacccgt tgtatatttt ccgaagaaga gacaagacta
     6001 ggtatgagcc ggacggtacc aagaccatag taagacccac attttcctac ttgggaggat
     6061 acacaatatc caacaacact ttatttcaga ttgcaagtta cggatgtacc gaaataaacg
     6121 gctatgaggg catgacccgc tttcgtgtca taaaccgacc ggaagggata tctctggaag
     6181 taaatataac ggtaaatgtg gattatgata taccggaaac tttgcgacag gtacagcaga
     6241 aaatatatga tgtgctggat tttaccaccg ctttgaatat gataagaatt gatgtgacag
     6301 ccaagaatat catgataaac ggggaagtat atccttagtg cggtacatat gtttacttga
     6361 caatacatat gttatgcatt aaactgtcga tatattttga tttatggagc gtgaaaatcc
     6421 ttaatggata gtgacagtat ataccgaagt atc
//
LOCUS       DAOPWC010000032         6400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_12068_length_6400_cov_3.129866, whole genome shotgun sequence.
ACCESSION   DAOPWC010000032 DAOPWC010000000
VERSION     DAOPWC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12068_length_6400_cov_3.129866"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKJ65_01350"
     CDS             complement(<1..300)
                     /locus_tag="PKJ65_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ40515.1"
                     /translation="MFKKIKSFFNTVFKKTKTDPGEVETKNFYGQDESEIPGYTPDHI
                     IFCDGLVKIYKSKDTEVVALQGLELEIKKGEMMAIIGNSGSGKSTLMNLIGGLDVP"
     gene            complement(300..971)
                     /locus_tag="PKJ65_01355"
     CDS             complement(300..971)
                     /locus_tag="PKJ65_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40516.1"
                     /translation="MAELLRTVDICRDFKQGSQTVHALVDVNVSIQPGTLTMLAGRSG
                     SGKTTLMNIAGALDRPTSGKIFFHDREISDLSENKRDEIRRKSMGFVFQSVALISVLN
                     AYENVEFGLRMAGVHPKKRDRLVKEALTFVGLERRMKHKPHQLSGGEQQRVAIARSIV
                     ARPELIFADEPTAELDTKTGLRIVRLFRHLVDEEKLTIIMTTHDPSMIELADNVIRLE
                     DGRVV"
     gene            complement(971..1825)
                     /locus_tag="PKJ65_01360"
     CDS             complement(971..1825)
                     /locus_tag="PKJ65_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ40517.1"
                     /translation="MNEGIPINHILNILSSYPQGMIISRSLANEYGVKEGQFMNYVYS
                     STVDKMPVENLVENALVVAVVDYWPAMTGERFIVMTRDNYFVMKDFDTKGRILIDKKE
                     GVPDSQVLADIKGKMGPEEAIDSIKFTDYNVTLAKKNSVLNGMNGILTLDFLISMIVC
                     CIGFIIFWIISVRQRVLQFGIFRAMGMNKGELYRMIFYEQLMISFVSVIAGIVIGGVA
                     SQLFVPLFERMSTLSNQYLPFATYRDGIDYIRIYVITGVSLIIGLSVLIRFISKLKID
                     QALKLGED"
     gene            complement(1866..3794)
                     /locus_tag="PKJ65_01365"
     CDS             complement(1866..3794)
                     /locus_tag="PKJ65_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ40518.1"
                     /translation="MLRLIFRKILNNKWMVICLIIGCAFAIAVAAAIPMYASGMYNTM
                     LVNEFKKEQVQFDTFPFKASYNVTFFGLQSDTVKRVYESLDSTFYNKYMANMPVEVMK
                     DCNSVSTKTYSVLDQNNYRIKQDNILKEEKPSYRNFTIKTDFCLTSVVDAETVFDVVG
                     GRMFRTDTEEGVIEAVISEYAAALYRMSVGDYYFVYNKPDDDDFIAKIKIVGIVKVNE
                     SSASFFTVAERAFITDFDIMQEMLKESQITLSQANWFAAFDYTTARLRYADDISQATQ
                     ALSTSSSVTGVIINTSNNYISIINNYNRDMQTMQKVFLLLIIPVILMIAMYVVMISRL
                     MMEREKSEIATLASRGASRNQIFLIYLGQGIVIAVVSFIFGILLARLLCRLLGAANGF
                     MEFVNRTAIEVRFESTSLVYAVVCALIYLIMILVPAYKASKYTIVQLKQSIADQSKRS
                     FWQVMFLDVILIGVSLYFLYDYEQMSKYIQDVQSAQVDFTLFLAVTMFILGLGLLFIR
                     LFPYLTELVFIIGKRHWKPASYNALMHVSRSGNYKHFIMIFLILTIGVGIFNANSGRT
                     LNENLEDNIRYEIGADASFVPTLIFLNDDGSFSYIPVKGGGLMSFAELYCNSDSVNNY
                     TTYYYYNTFKGAHYIYRN"
     gene            complement(3796..4797)
                     /locus_tag="PKJ65_01370"
     CDS             complement(3796..4797)
                     /locus_tag="PKJ65_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ40519.1"
                     /translation="MNKKAYLSVLLIILLICMTGCMSIFPKEDEMLLLPVPSTAPVEY
                     KTINPTKGDIVNSITILCNVANRYENLQDQFFSVDGYLEELFVENEQYVEKGDLIAIL
                     SDRKDKEQVENAYLNALNEFIRVQDLYVKNAATEYQFRRAEIAYNHAKEKYDDLYIYM
                     TNKELRASQSGFIVNIRSFPNDIAKDAPIGRTIRFCSIATGQYKSISGTTSAVNASKL
                     KPGAGVIVVSKDIEYNGYVLTSEGTNLSFGLDEMDAVTAGEVVQIRVIIDEVYDVLKL
                     PKYAVTIRDQKTAAVRVLKDNTPTNMEINIGLTSSSEVEILPGQGIDEDTMIITGTK"
     gene            complement(4930..5667)
                     /locus_tag="PKJ65_01375"
     CDS             complement(4930..5667)
                     /locus_tag="PKJ65_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ40520.1"
                     /translation="MKLTVLGNNGPFPAKDGACSGYVIESNNKDCGSGIKTLNSKGLV
                     ILDLGSGCLANFQKKFDISDISFIVITHLHFDHMSDLLVLRYALQVKGRILDVYLPKE
                     PENIVSVLKCDEFNLVHIDESSVIKRDSFVLRFKKTTHPVETYAVKINDRFVYSSDLS
                     QPEDLTDFAYGVDTLLIDSALLAKQKSKTKAAHLCAKECGMLAAKLNVKRLILTHFPP
                     YSDLEQYCMEASSYFSNTVAAEILGEY"
     gene            complement(5642..>6400)
                     /locus_tag="PKJ65_01380"
     CDS             complement(5642..>6400)
                     /locus_tag="PKJ65_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNZ40521.1"
                     /translation="YKAKILIHEQNAYPGITNKMLARRADVAMASFQSALKSLSKAKK
                     TVLTGNLIDESFSCFAKYQPSEGIKTVVIIGGSQGAMTVNNAVTGMINEYMKETDFKL
                     IFAPGKRHYEEVIGKITKTLKNVTVRDYIYDRDTVYASADLMVTRGGATTISEVLAMG
                     MPSIIIPSPFVPDNAQEKNAEQIFNRDACEIIYDNGEFNSKVLYDKIMNLLGNNDKLI
                     ALSENAYSLGIRDANQRVYEEFSEVVNEINRSGK"
BASE COUNT         1829 a         1487 c         1119 g         1965 t
ORIGIN      
        1 aggcacgtcc aggcctccta ttaagttcat cagagtggat ttaccgcttc cgctgttgcc
       61 gataatcgcc atcatctcgc ctttttttat ctccagttcc aacccctgca gagcaaccac
      121 ttcggtatcc ttggatttat atatctttac caacccgtcg cagaatatga tatggtcggg
      181 tgtataaccc ggtatctccg actcatcctg gccgtaaaaa ttcttggtct ccacctcgcc
      241 cggatcggtt ttggtttttt taaagacggt attaaagaat gattttattt tcttaaacat
      301 cacactaccc tcccgtcttc cagccttatg acattatcgg caagctcaat catgctcgga
      361 tcgtgcgttg tcattattat tgtcagtttt tcctcatcca caagatgccg aaacagtcgg
      421 actattctca aaccggtctt tgtatccagc tccgcggtcg gttcatcggc aaatattaat
      481 tccggccttg caactattga gcgtgcaatg gcaactcttt gctgctctcc tccggaaagt
      541 tggtgcggtt tatgtttcat tcttctttcc agaccgacaa atgtcaacgc ttccttaacc
      601 agcctgtccc gcttcttcgg atgcactccg gccatacgaa gaccgaactc gacattctca
      661 tatgcattga gtacagatat taaagctacc gactggaaaa caaatcccat gctctttctt
      721 cttatctcat cccgcttatt ctcacttaaa tcggaaatct ccctgtcatg aaagaatatc
      781 tttcccgaag tcggcctgtc caatgcaccg gctatattca taagcgttgt tttacccgac
      841 cccgaccttc ctgcaagcat agtaagcgta ccgggttgta tggatacatt tacatccaca
      901 agagcatgta ctgtctggct tccttgtttg aaatctctgc agatatccac ggttctcaat
      961 aactcagcca tcagtcttct cctaacttca gtgcctgatc tatctttaat ttggatatga
     1021 accttataag caccgataat ccgattatta acgatacacc cgttataaca taaatcctta
     1081 tgtaatcaat tccgtctctg taagtggcaa aaggaaggta ttgattgctt aaagtcgaca
     1141 ttctttcaaa taaaggaaca aacagctggc tggcaacacc gcctatcaca atacccgcaa
     1201 taaccgatac gaaagatatc atcagctgtt cgtagaatat cattctgtat aattcacctt
     1261 tgttcattcc cattgcacgg aaaataccga attgcaacac tctctgtctt accgatataa
     1321 tccagaagat tataaaaccg atacagcaca ctatcatgga tattaggaaa tccagtgtca
     1381 gtattccgtt catcccgttc agaacggaat tcttcttggc aagcgttaca ttatagtcgg
     1441 tgaattttat actgtctata gcctcttccg gtcccatttt tccttttata tccgccagaa
     1501 cctgactgtc cggaacacct tccttcttgt ctatcaatat tctgcctttt gtatcaaaat
     1561 ctttcattac aaaatagttg tcgcgtgtca taacgataaa gcgttcaccg gtcattgcag
     1621 gccagtaatc cacaaccgcc accacaagag cattctctac caaattttcg acgggcattt
     1681 tgtcaacagt cgatgaatac acataattca taaactgtcc ctctttcacg ccgtattcgt
     1741 ttgcgagcga tctggatatt atcatgccct gaggataaga ggacagaata ttcaatatgt
     1801 gatttatggg aataccttca ttcaaatctt tccttaattt tacaacattg ccgaattcgg
     1861 acggctcaat tccgataaat gtaatgggct cccttgaagg tattatagta ataatatgta
     1921 gtgtaattgt taacggaatc ggaattgcag tataactccg caaaagacat gagaccacca
     1981 cctttaacag gtatgtaact aaaagatccg tcatcattca gaaaaatcag ggttggtacg
     2041 aaagatgcat ccgcacctat ttcataacgg atattatcct ccagattctc attcagagtc
     2101 ctgccggaat tggcattgaa tatacccaca cctatcgtaa gaatgaggaa aatcataata
     2161 aaatgtttgt agttaccgct gcgggaaaca tgcatcaatg cattgtacga tgccggtttc
     2221 caatgtctct ttccgattat aaacacaagt tccgtcagat aaggaaacaa ccttataaac
     2281 aataacccca atcccaatat aaacatggtt accgccagga acaatgtaaa atccacctgt
     2341 gccgattgca catcctgtat atatttactc atctgttcat aatcgtagag gaaatataac
     2401 gagacaccta tcagtatgac atccaagaac ataacctgcc agaaggatcg cttggattgg
     2461 tcggctatac tctgttttag ctgaacaatc gtatatttcg atgctttgta tgcaggcact
     2521 aaaatcatta tgaggtatat cagagcacat actacggcat acaccagaga agtgctttca
     2581 aacctgactt caattgccgt tctgttgaca aattccataa atccgtttgc cgctccgagc
     2641 agacggcaca gtaaacgcgc cagtaatata ccgaaaataa aggaaactac cgctatgaca
     2701 ataccctgtc ccagatagat aaggaaaatc tgatttctgc ttgcacctct gctcgccaaa
     2761 gtggcaattt ccgatttttc tctttccatc atcagccggg aaatcattac aacatacatc
     2821 gctatcatca atatgaccgg gattatgagg agcaggaata ccttctgcat agtctgcatg
     2881 tccctgttat agttgtttat aatggaaata tagttgttcg atgtgtttat aataacaccc
     2941 gtaacactgc tggatgtgga taaagcctgc gtggcctgtg agatatcatc ggcatatctt
     3001 aagcgagcgg tggtgtaatc aaaagccgca aaccagttcg cttgacttaa agtaatctgc
     3061 gattctttta acatttcctg cataatgtca aaatccgtaa taaaagctct ttcggcaacg
     3121 gtgaaaaatg aagccgaaga ttcatttacc tttactatgc cgacaatttt aatttttgcg
     3181 ataaaatcgt catcgtcggg cttgttatag acgaaatagt aatcaccgac cgacattcta
     3241 tacaaagcgg cggcatattc ggatattacc gcttcaatca ctccttcttc ggtatcggtc
     3301 cggaacatac gtccgcccac gacatcaaat acagtctccg catccacaac ggaagtaagg
     3361 caaaaatccg tctttatcgt aaagtttctg tatgacggct tttcttcttt taaaatatta
     3421 tcctgtttta tcctgtaatt gttctgatcc agtacggaat aggttttggt ggaaacactg
     3481 ttacagtctt tcataacctc caccggcata ttagccatgt atttgttata gaaagtgcta
     3541 tcaagacttt cgtatacccg cttgacggta tcgctttgca gtccgaaaaa tgtcacattg
     3601 tatgaggctt taaacggaaa agtatcaaat tgcacctgtt cttttttaaa ttcgttgacc
     3661 agcattgtgt tgtacatacc cgaagcatac atgggaattg cagcagctac cgctatggca
     3721 aatgcacacc ctatgataag acagataacc atccatttat tattcaatat ctttctgaaa
     3781 ataagtctca gcatactact tggtccccgt tataatcata gtatcttcat caatgccttg
     3841 tccgggaagt atttccactt ccgaagacga agtcaatccg atgtttattt ccatatttgt
     3901 gggggtatta tcttttaata cccttaccgc agctgttttc tgatctctta tggtaacggc
     3961 atatttcggg agtttgagca catcataaac ttcatctatt atcactctga tttgcactac
     4021 ttctcccgca gttaccgcat ccatttcatc caatccgaag gaaagattcg ttccctcgga
     4081 tgttaacaca tacccgttat attcaatatc tttactgaca actatcacac ccgctccggg
     4141 cttcaattta gaggcattga cagccgatgt ggtaccgctg atgctcttat attgtcccgt
     4201 agcaatagaa cagaatctta tagtcctgcc gataggagca tcttttgcaa tatcattggg
     4261 aaaggagcga atattaacta taaagcccga ttgcgatgcc cgaagttcct tgttggtcat
     4321 gtaaatataa aggtcgtcat acttctcttt ggcatgattg taagctattt ccgcccttct
     4381 gaactgatac tcggttgcgg catttttaac atacaggtcc tgtaccctta taaactcgtt
     4441 caaagcgttc agatatgcat tttccacctg ctctttatct ttgcggtcgg atagaatagc
     4501 tattaaatcg cctttctcta catactgttc gttttccaca aacaattctt ccagatatcc
     4561 gtctaccgaa aaaaactgat cctgtaagtt ctcatatctg ttagcaacat tacacagaat
     4621 ggttatggag ttgacaatat cgccttttgt ggggtttata gtcttatact ccacaggagc
     4681 cgttgaggga acaggcaata acagcatttc atcttccttg gggaagattg acatacaccc
     4741 tgtcatacaa atcaataata ttatcaatag tacggaaaga tatgcttttt tattcatatg
     4801 aagtccttct gtaggcatta tagcccatta acctgtcaat taaaagcatt ttactgcaaa
     4861 acattatgaa cttttcatga acttttaata actctttttg aaattcgcag caaatattac
     4921 atatatccgt taatattctc caagaatttc cgctgccacg gtattgctga aatatgagga
     4981 agcttccata cagtattgct ctaaatcgga ataaggcgga aaatgtgtca gtataagcct
     5041 ttttacattt aattttgctg ccagcatccc gcattctttt gcacaaagat gagcggcttt
     5101 tgttttactc ttctgttttg caagcaaagc cgaatcgatt aacaaagtat caacaccgta
     5161 tgcaaaatcc gtcaaatcct ccggttgcga caaatccgac gaataaacaa aacggtcatt
     5221 aatttttaca gcatatgttt ccacggggtg tgtggttttc ttaaaacgca agacaaaaga
     5281 atctcttttt attaccgaag attcgtcaat atggacaaga ttaaactcat cgcatttcaa
     5341 taccgatact atgttttccg gctctttcgg caagtataca tccaatatcc tccccttcac
     5401 ctgaagagca tatcgcaaga ccaataaatc cgacatgtga tcaaaatgaa gatgggttat
     5461 tacaataaaa gatatatccg aaatgtcgaa cttcttttgg aaatttgcaa gacaaccgct
     5521 tcccaagtcc aaaattacaa gacctttact gtttaaagtc ttaattccgc ttccgcaatc
     5581 tttattattg ctctctataa catatcccga acaggctccg tcttttgcag ggaaaggtcc
     5641 gttatttccc agaacggtta atttcattta caacctccga aaactcctca taaactcttt
     5701 gattggcatc acgtataccc agcgaatagg cgttttccga cagagcaatc agtttatcat
     5761 tatttccgag aagattcatt atcttatcgt aaagaacctt ggaattaaat tcaccgttat
     5821 catatataat ctcacaggca tccctgttaa atatctgctc ggcatttttc tcctgtgcgt
     5881 tatccggcac aaaaggagaa ggtattatta tcgaaggcat acccatggca agtacttcgc
     5941 ttattgtggt agcacctccc ctggttacca taaggtcggc agatgcatat acggtatcgc
     6001 ggtcgtatat ataatcacgg acagtaacat tcttaagtgt cttggttatc tttcctataa
     6061 cctcctcata gtgcctttta ccgggagcga aaatcaactt aaaatccgtc tccttcatat
     6121 attcattaat catacctgta acggcattat tgacagtcat ggctccctgg cttccgccta
     6181 ttatcacaac ggttttaata ccctcggaag gttgatactt ggcaaagcaa gaaaatgatt
     6241 catcaatcag attgccggta agtacggtct tttttgcttt acttaaggat ttaagtgccg
     6301 actggaaact tgccatggca acatccgcac gccttgccag catcttatta gtaataccgg
     6361 gataggcatt ctgttcatgg ataagaatct tggctttgta
//
LOCUS       DAOPWC010000033         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_12219_length_6341_cov_3.890550, whole genome shotgun sequence.
ACCESSION   DAOPWC010000033 DAOPWC010000000
VERSION     DAOPWC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12219_length_6341_cov_3.890550"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKJ65_01385"
     CDS             <1..229
                     /locus_tag="PKJ65_01385"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162228.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HNZ40522.1"
                     /translation="SDADYIKTSTGFSTAGATREDIMLFKKHIKNGKLIKAAGGIRSL
                     NDANDFISLGSSRLGTSSIVRIVKDEEITSY"
     gene            347..1420
                     /locus_tag="PKJ65_01390"
     CDS             347..1420
                     /locus_tag="PKJ65_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ40523.1"
                     /translation="MKKLFSIVLVVVLLVSMLSLAGCQAKTYELAMITDVGDIDDKSF
                     NQGTWEGLRDYAVEHKITHKYYKPLEVSTDAYLSAIDLAVSSGAKVIVTPGFMFEEAI
                     FIAQDRYPEVMFILLDGQPQDADYNKRIEKNVYAVIYAEEHCGYLAGYAAVVDGYRNL
                     GYQGGMAVPAVCRYGFGFVQGANDAAKELGLAKGDVKIMYNYSGDFADTPASQTRAAG
                     WYQAGTEVIFAAGGKVGNSVMSAAEAANKAVIGVDVDQSAESPTVISSAMKGLATMVQ
                     YGLKEFYAGKFPGGQVVTVGAESNAVSLAPFSTSKWKTFSQADYDALFAKLANGTLAP
                     EKDNDRTNPTELKLDYVKLTFVE"
     gene            1601..3130
                     /locus_tag="PKJ65_01395"
     CDS             1601..3130
                     /locus_tag="PKJ65_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40524.1"
                     /translation="MDYIIEMLNIRKEFPGIVANDDITLRIKKTEIHALLGENGAGKS
                     TLMSVLFGLYKPEAGIIKLRGKEIDISDPKTASHLGIGMVHQHFKLVEDFTVLENIIL
                     GAETVKNGFLKMDEAKKKVMELSNRYKLYIDPDAKVSTITVGMQQRVEILKMLYRENE
                     ILIFDEPTAVLTPQEIDELMGIMKELSKEGKSLIFITHKLEEIKKVADRVTVLRRGKC
                     IGTYNVQDVTKESLSEMMVGRKVQFTVDKKDITPGETIFEVKNLSMKTKGSTKNTLNK
                     ISFKVRKNEIVCIAGIDGNGQSELVYALTGIRHFDQGQILVHGEDWTKKSIRYRNSHG
                     MSHIPEDRHKYGLILEYSMAYNLILQTYFTDRFQKRGFLKNDSIHEYADGLIKKYDIR
                     SGQGARTVTRSMSGGNQQKVILAREIDKDPSIMIAVQPARGLDVGATEFIHKRLVEQR
                     ESGKAVLLISLELEEIMNLSDRILVIYEGELVGDLDPKKTTIQELGLYMAGAKRDEVK
                     A"
     gene            3145..4296
                     /locus_tag="PKJ65_01400"
     CDS             3145..4296
                     /locus_tag="PKJ65_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ40525.1"
                     /translation="MNQFFDRLSRKKGTGSFISSLVAIGIGLVIGLIILLISNPREAF
                     AGFFTMLIGGFNDGTRSFGNVFYLATPIIMTGLAVGFAFKTGLFNIGASGQFIVGAYV
                     AVLIGVKLPQLGGFQWVLGILAAMAIGTIWGAVPGLLKAYLNVHEVIASIMMNYIGMF
                     MVNYLIKQTVLNVYTNRSLAVAPTANIPKMGLTAIFPGSSINGGLLVAILFVILMYII
                     LEKTTFGYELKACGHNRFAAKYAGINENRNIVLSMAISGMLAGVGGALHYLSGAGVYI
                     EVVDVLAAQGFTGISVSLLGMSNPIGVFFAGIFIAWLQIGGFYMQRYSFVPEFIQIIT
                     AIIIYCSAASLLIRNYLELRQLKKDEKLSADSDNDTADIVNVENQGGGN"
     gene            4296..5255
                     /locus_tag="PKJ65_01405"
     CDS             4296..5255
                     /locus_tag="PKJ65_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ40526.1"
                     /translation="MDMLYLLLRQTMYFSIPLLIVALGGMFSERSGVTNIALEGIMVM
                     GGFTGIYFINQMQSQMSGQWLLVLAIFIAMAGGLLLSLPHAYASVNLKADQVISGTAL
                     NMFAPAFVIYIARMIQPSGIANVSFKNTFFIHKVPVLCDIPVIGDILFRNTYITTFLG
                     FFILIVSAVVLYKTRFGMRLRACGEHPQAADSVGINVYKMRYIGVMISGVLAGLGGLV
                     LTVPISTEFKGAVNGYGFLALAVLIFGQWKPMRILWASLFFGFMQTISAAYSAIPFLL
                     NLNIPSEFFKMTPYIATLIVLAFSSKSSKAPKAVGQPYDQGAR"
     gene            5259..>6341
                     /locus_tag="PKJ65_01410"
     CDS             5259..>6341
                     /locus_tag="PKJ65_01410"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNZ40527.1"
                     /translation="MRMYDIIQRKRDKYPLKDEEIRFFVKGYTGGSIPDYQAAALLMA
                     IFLNGMTDHETSVLTLAMAQSGDMIDLSAVNGITVDKHSTGGVGDKTSLIVAPCVASL
                     GFKVAKMSGRGLGHTGGTVDKLESIPGYRTSLDRQEFIDNVNRIGIALVGQSGNLAPA
                     DKKIYALRDVTATIDCLPLIVSSIMSKKIAAGSQNILLDVKVGTGSFTPKLEDAVILA
                     QKMVDTGKLAGRNIAALITNMDVPLGNAIGNSMEIIEACEILKNKGPSDLREVCVELS
                     AYMVSLCTQDSYENCRRKVLQTFADGSAFEKFRQLVSAQGGDVAFIDNTDKFKKAFYT
                     HDVLSLRSGFISSMDTANIGRVSLMLG"
BASE COUNT         1937 a         1188 c         1516 g         1700 t
ORIGIN      
        1 aagcgatgcc gactatataa agacatcaac gggcttttcc acagcaggtg ccacaagaga
       61 agacattatg ttgtttaaga agcatattaa aaacggcaag ctcataaaag ccgccggagg
      121 cataaggtct ttaaacgatg caaatgattt tatcagtctc ggctcttccc ggctgggcac
      181 atcaagtata gtaagaattg ttaaggatga ggaaattaca agttattgaa atgttaaaga
      241 aatgtgtcaa gaatgtgaac tttttgtttt ttcccccttg tttataaact ataaatctga
      301 tagtatttat atgtgaattt cattatatca aaaaaggagt ataattatga aaaaactatt
      361 tagtatcgta ttggtagtgg tgcttttggt ttctatgctg tccttagcag gctgccaagc
      421 taaaacctat gagctggcaa tgattaccga cgtaggagac attgacgaca agtcctttaa
      481 ccagggaaca tgggaaggat tgagggatta tgcggtagag cataaaatta cgcataagta
      541 ttacaaacct ctcgaagttt ccacagatgc gtatctttca gctatcgatc ttgcagtatc
      601 atccggagct aaagtcatcg taacaccggg atttatgttt gaagaagcta tttttattgc
      661 ccaagacaga tatccggaag ttatgttcat acttttggac ggtcaaccgc aagatgcaga
      721 ctataacaag cgtattgaaa agaatgttta tgcagttata tatgcggaag agcactgcgg
      781 ttaccttgca ggttatgcgg ctgtagtaga cggatataga aatttaggtt atcagggcgg
      841 aatggctgtt ccggcagttt gcagatacgg attcggtttt gtacagggtg ctaatgatgc
      901 agctaaagaa ttaggacttg caaaaggcga tgttaaaata atgtacaact attccggaga
      961 ctttgccgat acaccggcat cacagacaag agctgctggc tggtatcagg caggaaccga
     1021 agttatcttt gcagcaggcg gaaaagtagg aaactccgtt atgtcggcag cggaagcagc
     1081 caataaagcg gttataggtg ttgacgttga ccaatcggcg gaatcgccta cggttatctc
     1141 ttcggcaatg aagggcttgg caacaatggt tcagtacgga ttaaaagagt tctatgcagg
     1201 aaaattcccc ggcggacaag tcgtaacagt cggtgccgaa tccaatgcgg taagccttgc
     1261 accgttctca acttcaaaat ggaaaacctt ctcacaggca gactatgatg cattatttgc
     1321 aaaattggct aacggaacct tggctccgga aaaggataac gacagaacaa atcccacgga
     1381 actcaaactg gattatgtta aattaacttt tgttgaataa gagtaaaaca aggaagaaac
     1441 aaaaactgcc gtgtaaaagc ggcagttttt ttatcgttaa ttactatatg cttctgtttt
     1501 tttctttttt ttactttagc cgttattctt tatttattat tcttctcaaa taatatataa
     1561 taatgtaagt taaaaacact tatagccaag gagcaaattt atggattaca tcatcgagat
     1621 gttgaacatc aggaaggaat ttcccggtat tgtcgctaac gatgatatca ccctccggat
     1681 taaaaaaacc gaaatacatg cacttttggg tgagaacgga gcaggaaaaa gcacccttat
     1741 gagtgtactt ttcggtctgt ataaaccgga ggcgggcatc ataaaattaa gaggcaaaga
     1801 gattgacatt tccgatccca agacggcaag tcatctgggc ataggaatgg ttcatcagca
     1861 cttcaaactg gtggaagatt ttacagtact ggaaaacata atattgggtg cggaaacggt
     1921 aaaaaacggc tttcttaaga tggatgaagc aaaaaagaag gtaatggaac tgagcaaccg
     1981 ctacaagctg tatatagatc ccgatgcaaa agtgtcgacc attaccgtcg gcatgcagca
     2041 acgagtggaa atacttaaaa tgctgtacag ggagaatgaa atacttatct ttgacgaacc
     2101 aacggcggtt cttactccac aggaaattga tgaattgatg ggcataatga aagaactgtc
     2161 caaagaagga aagtctttga tattcattac tcataaactg gaggaaataa agaaagtcgc
     2221 cgacagggtt accgttttaa gaagaggaaa atgcatcggt acctataacg tacaggatgt
     2281 caccaaagaa tccttgagtg aaatgatggt cggaagaaag gtgcagttta cggtagacaa
     2341 aaaagatatt actccgggtg aaaccatctt tgaagttaaa aacctttcca tgaagaccaa
     2401 aggctcaacc aagaatactc tgaataaaat atctttcaag gtcagaaaaa atgaaatagt
     2461 gtgcatagcg ggaatagacg gtaacggaca gagtgagctg gtctatgcgc ttacaggcat
     2521 acgacatttt gaccaagggc agatacttgt tcacggagag gattggacca agaaaagtat
     2581 aagatacaga aattcccacg gaatgagcca tatccccgaa gaccggcata aatacggact
     2641 tatactggaa tacagtatgg catataacct tatacttcag acatatttca ccgacaggtt
     2701 tcaaaagaga ggttttctca agaatgacag tatacatgaa tatgcagacg gtttgattaa
     2761 aaagtacgac atacgaagcg gacagggcgc acggaccgtt acaagatcca tgtcgggagg
     2821 taatcagcag aaagtaatat tggccagaga aatagacaaa gacccttcca taatgatagc
     2881 cgtccaaccg gcaagaggtc tggatgtcgg agccacggaa ttcattcata agcgactggt
     2941 agagcaaaga gagagcggaa aagcggtttt gcttatttca ctggaactgg aagaaataat
     3001 gaacttaagt gacagaatac ttgttatata tgaaggagaa ttggtgggtg atcttgatcc
     3061 taaaaagacc accatacagg agttgggcct gtatatggca ggagcaaaaa gagatgaggt
     3121 gaaagcatga gtaaccgaaa ttccatgaat cagttttttg acagattaag cagaaaaaaa
     3181 gggacgggaa gctttatttc ctctttagta gccataggaa taggtcttgt aataggactt
     3241 ataattcttc taatatccaa tccccgggaa gcctttgcgg gattttttac catgcttatc
     3301 ggaggtttta acgacgggac aagaagtttc ggaaatgtct tctatcttgc cacacctatc
     3361 ataatgacgg ggctggcggt gggattcgca ttcaagaccg gactgttcaa tataggagca
     3421 agcggtcagt ttatcgtagg ggcatatgtt gcggtactta tcggagtaaa acttcctcag
     3481 ttgggaggct tccagtgggt tttgggcata ttggccgcca tggctatagg gacgatatgg
     3541 ggagcagtac cgggactttt aaaggcttat ctcaatgttc acgaagtaat agcctctatt
     3601 atgatgaact acataggcat gtttatggtt aactacctta taaaacaaac tgtgctcaat
     3661 gtatatacca acagatcgct tgccgtggct ccgaccgcaa acatacccaa gatgggtctt
     3721 acggcgatat tcccgggatc cagtataaac ggcgggttac tcgtagcaat actgtttgta
     3781 atactcatgt atataattct ggaaaagacc actttcggat atgaacttaa agcctgcgga
     3841 cataacaggt tcgcagcaaa atacgcagga ataaatgaaa acagaaacat agtcctttcc
     3901 atggcaatat ccggaatgct tgcaggtgtc ggaggggcct tacactattt gtccggtgcg
     3961 ggcgtatata tcgaggtcgt ggatgttctg gccgctcagg ggttcacggg aatatccgtt
     4021 tcacttttag gcatgagcaa tcccataggc gtcttcttcg caggcatatt catagcatgg
     4081 ctgcagatag gcggcttcta tatgcaaaga tacagctttg ttccggagtt catacagatt
     4141 ataacggcaa ttataattta ctgttccgca gcatctctgc ttattcggaa ctatctggaa
     4201 ttgcggcaac ttaagaaaga cgagaaactt tccgccgatt ccgataatga cacggcggat
     4261 attgttaatg ttgaaaatca aggaggtggc aactgatgga tatgctatat cttttactca
     4321 gacagaccat gtatttttcc attcctttac ttatagtggc tttaggaggc atgttttccg
     4381 aaagaagcgg cgtaaccaat atagcgcttg aaggaatcat ggtaatggga ggatttaccg
     4441 gcatatattt tatcaatcaa atgcaatcgc agatgagcgg gcagtggctg ctggttctgg
     4501 ctattttcat agccatggcg ggaggtttgc ttttatctct tccgcatgca tatgcttcgg
     4561 ttaatcttaa agccgatcag gtcatatcgg gaaccgcact taatatgttt gcacccgctt
     4621 tcgtcattta tatagccaga atgatacagc cttcgggaat agccaatgtt tcctttaaaa
     4681 acactttctt tatacacaaa gttccggtgc tttgtgacat accggtaata ggggatattc
     4741 ttttcaggaa tacatatata accacattct taggcttttt catacttata gtttccgcag
     4801 tagttctgta taaaacccgt ttcggaatga gattgagggc ttgcggagag caccctcagg
     4861 ctgccgattc ggtgggaatt aatgtatata aaatgagata tataggcgtt atgatttccg
     4921 gagtgcttgc cggactgggc gggcttgttc tgaccgttcc catatccacc gagtttaagg
     4981 gagccgttaa cggatacgga tttttggcat tggccgttct tattttcgga cagtggaaac
     5041 ctatgagaat attgtgggca tctttgttct tcgggtttat gcagacaata tcggcagcct
     5101 attcggcaat acccttcctg ttgaatctga atataccttc ggaattcttc aagatgactc
     5161 cgtatatagc tacacttata gtgcttgcat tctcgtcgaa atccagtaaa gctccgaaag
     5221 ctgtaggtca accgtatgac cagggggcaa gataagtcat gagaatgtat gacatcatac
     5281 aaagaaaaag agataagtat ccgctgaagg atgaggaaat ccgattcttt gtaaaaggat
     5341 ataccggcgg ttccataccc gattatcagg ctgccgcttt actgatggca atctttctca
     5401 acggcatgac cgatcatgaa acttccgtgc ttactctggc tatggctcaa tcgggagata
     5461 tgattgattt atcggccgta aacggaatca ccgtggacaa acacagtacc ggcggagtgg
     5521 gagacaaaac gtcattgata gtggctccct gtgtggcttc tttgggcttt aaagtagcca
     5581 aaatgagcgg aagagggctg gggcataccg gaggtacggt agataaactg gagtccattc
     5641 ccggctatag gacctctttg gacagacagg agtttatcga taatgtaaac aggataggaa
     5701 tagcgttggt cggacaaagc ggtaatcttg ctcccgccga caaaaagata tatgcattgc
     5761 gtgatgtgac ggcgactata gattgtcttc cgcttatagt gtccagcata atgagcaaaa
     5821 aaatagcggc cggatcacaa aacattctgt tggatgtcaa ggtgggaacg ggatctttta
     5881 cgcccaaact ggaagatgcc gttattcttg cgcagaaaat ggtagatacg ggaaagcttg
     5941 cgggacgaaa cattgccgca ttgataacca acatggatgt acccttggga aatgcgatag
     6001 gcaacagcat ggagataatc gaggcctgtg aaatactcaa gaacaagggg ccttccgatt
     6061 tgagggaagt gtgcgtggag ctgtctgcat atatggtgtc gctttgcacg caagattctt
     6121 atgaaaattg cagaagaaaa gttttacaaa cctttgcgga cgggtcggct tttgaaaaat
     6181 tcagacaact ggtttccgct cagggtggcg atgtggcatt tatagataat acggataagt
     6241 ttaaaaaagc gttttacaca catgatgtat tatccttacg ctccggtttt ataagttcaa
     6301 tggataccgc taatataggc agagtctctc tgatgctggg t
//
LOCUS       DAOPWC010000034         6259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_12431_length_6259_cov_3.473727, whole genome shotgun sequence.
ACCESSION   DAOPWC010000034 DAOPWC010000000
VERSION     DAOPWC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12431_length_6259_cov_3.473727"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKJ65_01415"
     CDS             complement(<1..628)
                     /locus_tag="PKJ65_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNZ40528.1"
                     /translation="MTIWQAILLGIIQGLTEFLPVSSSGHLVLFEKILGIETPTLTFN
                     IAVHFATLVAVCVVFWKDIIELVKKPVQKLTLWLIIATIPAALAGVLFNDFFEGLNDS
                     GATLGIGFLITAVLLIVSSVLKPGTVEKENMKWYDALIIGVIQMVAIVPGISRSGSTL
                     TGGLFRKLKKEDAVKFSFLLSIPVILGGFILELYGAVKVGLGNIEVLPI"
     gene            complement(625..1896)
                     /gene="clpX"
                     /locus_tag="PKJ65_01420"
     CDS             complement(625..1896)
                     /gene="clpX"
                     /locus_tag="PKJ65_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNZ40529.1"
                     /translation="MSDEIKKIRCSFCGKSEDRVQRLISGPGVYICNECVDLCSDLLD
                     TELGYDDEEEEVTETVIDQVPHPEEIKRQLDTYLIGQDDAKKAISVAVYNHYKRISYN
                     VAHEPDDVEIQKSNILMLGPTGTGKTYMAQTLAKILNVPIAISDATTLTEAGYVGDDV
                     ENILLRLIQAADYNLQRAQRGIVYIDEVDKIARRGDNPSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGRKHPTQEYIQIDTTNILFICGGAFQGIDEIILNRLGKRAIGFGAEIH
                     KTNSVEKDRILKHIETQDLLKYGLIPEFVGRLPVIVTLEQLTEDILVSILKEPKNALV
                     KQYKKILEMDGCELVFEEKALRAIAKKTLERKSGARGLRSIIESALMEVMYSVPSDPS
                     IVKCIVEESTILNNTPPKLVYKGNLLEENVS"
     gene            complement(1910..2491)
                     /gene="clpP"
                     /locus_tag="PKJ65_01425"
     CDS             complement(1910..2491)
                     /gene="clpP"
                     /locus_tag="PKJ65_01425"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNZ40530.1"
                     /translation="MNTIPYVIEQTSRGERSYDIFSRLLKDRIVFLGSEVNDIAASLI
                     IAQMLFLEHEDPEKDIKLYINSPGGSVTSGLAIYDTMQLVKCDVSTMCVGLAASMGAF
                     LLAAGTKGKRYALPNSQIMIHQPSGGIQGQATDIRLHTDWLLKVKDNLNRILAEKTGQ
                     PLEQIAKDVERDYFMSSADAKQYGIIDEVLNKR"
     gene            complement(2515..3930)
                     /gene="tig"
                     /locus_tag="PKJ65_01430"
     CDS             complement(2515..3930)
                     /gene="tig"
                     /locus_tag="PKJ65_01430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ40531.1"
                     /translation="MESKIVKKEGYKVEMEIALTKEEFNGYITKAYYKDKNKYTIQGF
                     RKGKAPQYLIERIYGKGVFYDTAVDMALNAEYPKAIDDLKLDVVDYPHVDLKDMDEEN
                     GITFSITVDVKPDVELGKYKGVEIKKAEVEVSDKDVEDALNREADKNSRMVSVEDRPV
                     KDGDTVIIDYEGFADGVAFEGGTAEKQQLVIGSKTFIPGFEEQIIGHNINDEFDVNVK
                     FPDEYHAEELKGKQAVFKVVLHEIKVKELPVIDDEFIKDISEYDTVEQYKEETKKKML
                     EERQKRAEDSMRSAALATACKNAKVDIPDAMIERATDNIIDNMRMEMQYSGYTLEQYL
                     EYTGMKMEDIRSQYKEQAGMMVVRDLVVEAIKNVENIQVTDEEIEELLNKEAETYKME
                     PDRIKQAIGDYKAYYTDKIANDKVVDLIYSSAKKVKEVKEDKKDKEVKEKKSVDKKKK
                     QDGEPEKKAASRKTAEDKSEK"
     gene            complement(4162..>6259)
                     /locus_tag="PKJ65_01435"
     CDS             complement(4162..>6259)
                     /locus_tag="PKJ65_01435"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ40532.1"
                     /translation="PFTAYDRTLLYYTFNVTEYLRKGSNMLGVILGNGFFNYPTIDQW
                     NTNAASWRGSPKMICELYITTKNNKQICIPSDITWESHMSPITFNSLRNGEFYDARLE
                     IPSWCQPDALVDDWVNVSISRDPGGLLKANEMPSVKVDREYPAISVKNVAKNTYLFDF
                     GQNMAGFVRIKVTGPKGAEFVIKMNEKLKDDGTLDTAYNSGFTKTGEFQTDKYIKKGD
                     GEEIWQPRFVYHGFRYAQIEGFTGQPDLSSATAVFLHTAVKEIGQFSCSDDNINQIHK
                     NTRMATYSNMYGLLTDSPHREKNAWTGDTNVSTEQILFNYMSGPLWMKWLNDIKDSMK
                     AKGNIPCMVPTGGWGYNWGNGPDYSSVLTWLPDSMHTYYADKYVIRKYYQYMKRNMKY
                     MLSMMQNDVCVNDYGVGDWCAPFEGPAISVNMSSFKAPIDLTDTACMYTMANVLEKFA
                     KMLGHRDDAKEFNKTAKRIKAGFRKTFYDTQTGRLKGDGQTCYAAMLYHNLYEDDKEY
                     ELVYKHLLRTIDEADGHLDYGILGNKYVNNILGIRGRADLIYSMITKETFPSFVHEIK
                     SLGATTLYETWNGEGSRNHHMFGDISATFFKYFAGIRPDPDYPGFKRFTIAPAFNTPL
                     VNVDAVFESMYGTIRSSYVKAGNTITMDIEIPTNTCAVLKLGAGMEIYEDNNLLGKGY
                     MEMESGKYKLEIRKTV"
BASE COUNT         1565 a         1504 c         1075 g         2115 t
ORIGIN      
        1 taatcggcaa cacttcgata tttcccaatc cgaccttaac agcaccatat agttccagaa
       61 taaatccgcc cagtattaca ggtattgaaa gcagaaaaga aaacttaacc gcatcttctt
      121 ttttcagttt cctgaataat ccgcccgtaa gagtggagcc cgatctggag attcccggta
      181 caatcgccac catctgtata accccgataa tcagagcatc ataccatttc atattttctt
      241 tctctaccgt tccgggtttt aatacggagg acactatcag taaaaccgcg gttattagaa
      301 accctatacc cagagtagct ccgctgtcat ttaatccttc aaaaaagtcg ttaaacagaa
      361 cacccgccaa agccgcagga attgtagcta ttatcagcca taatgtgagt ttctgaacag
      421 gtttcttaac caactcaata atatctttcc aaaacacgac acagaccgct accaatgttg
      481 caaaatgcac ggctatatta aatgtaagag tgggtgtttc tatacccagt atcttttcaa
      541 acagcactaa atgaccggaa ctggataccg gaagaaattc cgttaaaccc tgaattattc
      601 ctaatagaat tgcttgccag atagtcatga cacattttct tccaaaagat tacctttata
      661 cactaatttg ggaggtgtat tgttcaatat ggtactttcc tccactatac actttacaat
      721 ggacgggtcg gacgggaccg aatacattac ttccataagt gccgattcta taatggagcg
      781 taatccgcgc gctccgctct ttcgctccaa agtcttctta gctatggcac gtaatgcctt
      841 ttcttcaaat accagctcgc aaccgtccat ttccaatatc tttttatatt gttttaccaa
      901 ggcattttta ggttccttca gtatagaaac aagaatatct tcggtaagct gttccagcgt
      961 aacgataacc ggcaatctgc ctacgaactc gggaataagt ccgtatttca acaagtcctg
     1021 cgtttcaatg tgtttcaaaa tcctgtcttt ttccacactg tttgttttat gaatttccgc
     1081 tccgaaacct attgctcttt tacccaatct gttcaatatt atctcatcta taccctggaa
     1141 tgctccacca catatgaaca gtatattggt agtatctatc tgtatatatt cctgtgtcgg
     1201 atgctttctt cctccctgag gaggtacatt ggctacagtc ccttccagta tcttcaataa
     1261 tgcctgctgt actccctcgc ccgaaacatc tctggttatt gaagggttat cgcctcttct
     1321 ggctattttg tccacttcat ctatgtaaac aattcccctt tgggctcttt gcaggttata
     1381 gtccgcggcc tgtataagcc ttaacaatat attctccaca tcatctccca cataaccggc
     1441 ttcggtaagc gttgtagcat cggagatggc tatggggaca ttgagtatct tggcaagagt
     1501 ctgtgccatg taggttttgc ccgtacccgt aggacccaac ataagtatgt tgcttttttg
     1561 gatttccaca tcatcaggct catgagcaac attgtaactt atccttttat agtgattgta
     1621 taccgctacg gatatggcct tttttgcatc atcctgacct atcagatagg tatccagctg
     1681 tcttttaatc tcttcgggat gaggaacttg atctataacc gtttccgtta cctcttcctc
     1741 ttcatcatca tatcccagtt cagtatcaag aagatcggaa cataaatcca cacactcatt
     1801 gcatatatag acgccgggac cggagataag tctctgaacc ctgtcttcgg atttaccgca
     1861 aaaagagcat ctgatcttct ttatttcgtc tgacattcaa ttctcctttt tatctcttat
     1921 tcaggacttc atcaattatg ccgtactgtt ttgcatcggc tgaagacatg aagtaatctc
     1981 tttcaacatc ttttgctatc tgctctaagg gttgcccggt tttttccgcc aatatcctgt
     2041 tgagattatc ctttactttc aacagccaat cggtatgaag gcgtatatcg gttgcctgac
     2101 cttgtattcc tccggacggc tgatgtatca ttatctggct gttgggcagg gcatatcgct
     2161 tgcctttggt acctgccgca agcagaaacg ctcccatgga agcggccaat ccgacacaca
     2221 ttgtggaaac atcgcattta acaagctgca tggtgtcata aattgccaat cccgatgtaa
     2281 ccgatccgcc cgggctgtta atgtacaact ttatgtcttt ttccggatct tcatgttcca
     2341 gaaacagcat ctgagcgatt ataaggcttg ctgcaatatc attgacctca cttcccaaga
     2401 atactatcct gtccttcagc agtctggaaa aaatgtcata tgacctctct cctctgctgg
     2461 tctgttcaat tacatatggt atggtattca tcatttatcc ttccttgtcg gttattactt
     2521 ctccgactta tcttccgcgg ttttcctgga agccgctttc ttttcgggtt ccccgtcctg
     2581 tttctttttt ttatctacgc tctttttctc tttaacttct ttatcttttt tatcttcttt
     2641 aacttcctta accttctttg ctgaggaata tattaaatcc accactttat cgttagcaat
     2701 tttatcggta tagtatgcct tgtagtcacc tatggcttgc tttatccgat caggttccat
     2761 cttgtatgtt tccgcttctt tgttaagcag ttcctcaatc tcttcgtcgg taacctgaat
     2821 gttttcaaca ttcttaatcg cttccactac cagatcgcga actaccatca ttcctgcctg
     2881 ttccttatat tgactgcgga tatcttccat cttcattccg gtatattcca gatattgctc
     2941 caaggtatat ccgctgtatt gcatttccat cctcatattg tcgataatat tgtcggtcgc
     3001 tctttctatc atggcatcag gtatatcaac cttagcattt ttgcatgcgg ttgcaagtgc
     3061 tgcggaacgc atggaatctt ccgctctttt ctgtctttct tccagcattt tcttttttgt
     3121 ttcttcctta tactgctcaa ctgtatcgta ttcgcttatg tcttttataa actcatcatc
     3181 gataaccggc aattccttga cttttatctc atgaagtact accttgaaaa cagcttgctt
     3241 gccttttaat tcctccgcat gatattcatc gggaaacttg acattgacat caaactcgtc
     3301 attaatgttg tgcccgataa tctgctcttc aaatcccggt ataaatgttt ttgaacctat
     3361 taccagctgt tgtttttccg ccgttccgcc ttcaaaagca acaccgtccg caaatccttc
     3421 gtaatcaatt attacggtat ctccatcttt tacgggacga tcttcaacag ataccatacg
     3481 ggagttttta tctgcttctc tgttgagggc atcttccaca tctttatcgc ttacttccac
     3541 ttccgccttc tttatctcaa cacctttgta tttacccaat tccacatcgg gtttgacatc
     3601 cacggtaata gagaaggtaa ttccgttttc ttcatccata tcttttaaat ccacatgagg
     3661 ataatccact acatccaatt tgaggtcatc tattgctttg ggatattccg cattcaaagc
     3721 catatccacc gctgtatcat aaaaaacccc tttcccgtaa attctttcaa tcagatactg
     3781 aggagctttc ccttttctga agccctgtat cgtatactta ttcttgtctt tgtaatatgc
     3841 ctttgtaata tatccgttaa attcctcttt tgtaagagca atctccattt ccactttata
     3901 gccttctttt tttactattt tgctttccat tacatacctc cgccttcttt gcgtcgcagt
     3961 ttatattaac atatggtaca ccttaaatca ataaaattaa ataaataccc tgaattatta
     4021 catattatgt ctatacattc cggcataaac agactgtccc gtttcatatg tttcatttag
     4081 tatgaaaaaa cttttgttat agacaaacac aacactgcac ttttttggtg cagtgttgtt
     4141 attctgtttg caatgtgttt gttaaaccgt ctttcttatc tccagcttgt attttcccga
     4201 ctccatctcc atataccctt ttcctaaaag attgttgtct tcataaatct ccatccctgc
     4261 tcccagttta agaacagcac aggtattggt cggaatctca atatccatgg ttatagtatt
     4321 acccgctttg acataggaag aacgtatggt tccgtacatg ctttcaaaaa ctgcatctac
     4381 atttaccagc ggagtattaa atgccggagc aatggtaaac ctcttaaaac ccggatagtc
     4441 gggatcgggt cttatgcccg caaagtactt gaagaatgta gcggatatat caccgaacat
     4501 gtgatggttt ctggaacctt ctccgttcca ggtctcatac aaagtggttg cacccaacga
     4561 ttttatttca tgtacgaaac tcgggaatgt ttcttttgta atcatggagt agattaaatc
     4621 ggcacgacct cttattccca agatattgtt cacatattta ttacccaata ttccgtaatc
     4681 cagatgtccg tcagcttcat caatagttct gagaagatgc ttgtatacca gttcgtattc
     4741 tttatcgtcc tcataaagat tatgataaag cattgcggca taacaggtct gtccgtctcc
     4801 tttaagcctg cctgtttgag tgtcatagaa agtttttctg aaaccggctt taattctctt
     4861 tgcagtttta ttgaattcct ttgcatcgtc acgatgtcct aacatttttg caaatttctc
     4921 cagcacattg gccatggtat acatacatgc cgtatcggtt aaatcgatag gtgctttaaa
     4981 cgacgacata ttgaccgaaa tagccggtcc ttcgaaaggt gcacaccagt cgcccacccc
     5041 gtagtcattg acacatacat cgttctgcat catggaaagc atgtatttca tgtttctctt
     5101 catgtactga tagtatttgc gtataacata tttatccgca taatatgtat gcatgctgtc
     5161 gggaagccat gtcagtacgg aagagtagtc cggtccgttc ccccagttat atccccatcc
     5221 tccggtcggt accatacagg gtatatttcc ttttgccttc attgaatctt taatatcgtt
     5281 aagccacttc atccacaagg gaccggacat atagttgaag agaatctgct cggtggacac
     5341 attggtatct cctgtccatg cgttcttctc tctgtgaggc gaatcggtca ataaaccgta
     5401 catgttggaa taggtggcca ttctggtatt cttatgaatt tgatttatat tgtcgtcgga
     5461 acaggagaac tgccctattt cctttactgc ggtatgcagg aatacagcgg ttgcacttga
     5521 caaatccggc tggccggtaa atccttcaat ttgtgcatac ctgaagccgt ggtatacaaa
     5581 tctgggttgc cagatttcct ctccgtcacc cttcttgata tacttatcag tctggaattc
     5641 acccgtcttt gtgaaaccgc tgttataggc ggtatccaat gtgccgtcat ctttgagttt
     5701 ttcattcatc tttataacaa actcggctcc tttgggaccg gttactttta ttctgacaaa
     5761 tcccgccatg ttctgtccga aatcaaacaa atatgtgttt ttggctacat tcttaacact
     5821 gattgccgga tattctctgt caacttttac cgaaggcatt tcatttgctt tgagtaatcc
     5881 tcccggatcg cgtgatatac ttacatttac ccagtcatca actaatgcat ccggctggca
     5941 ccatgatggt atttccagcc tcgcatcata gaactctccg tttcttaaac tgttgaaagt
     6001 aatgggactc atatgacttt cccatgtaat atccgaagga atacatatct gcttattatt
     6061 cttggtggtg atatacaatt cgcatatcat cttgggactg cctctccagg aagcggcatt
     6121 ggtattccac tgatcaattg taggataatt gaagaatccg tttcctaaaa taacgcccag
     6181 catattggaa cccttacgca agtattcggt tacattgaaa gtgtagtaca gaagagttct
     6241 gtcatatgcg gtgaaaggc
//
LOCUS       DAOPWC010000035         6165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_12665_length_6165_cov_3.405892, whole genome shotgun sequence.
ACCESSION   DAOPWC010000035 DAOPWC010000000
VERSION     DAOPWC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12665_length_6165_cov_3.405892"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1846
                     /locus_tag="PKJ65_01440"
     CDS             200..1846
                     /locus_tag="PKJ65_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40533.1"
                     /translation="MRYINRVFQSSGQIDVQSVKNKSLRLAILFMVLLLLLTSCGPAV
                     TPRPSGVATLTPTTPSETPAPSETGTPVVEDPVPSEEPLPQPKTMEIDGVTYEMAPLP
                     EFPEPEELDFAAEKKKYQEDEDYSANYLSTVRLISYKEDKVLGYRTEVTARNANKETL
                     KFYPTEYQFYNFFKEYMGEIVNVIWYFDGNAYDENDKELYCCAYDMYIDEETNQNDNF
                     VEGKLTSYKLNSDYDIRITSGVVKLSERIDDLFLVLYVLGEDGYLYDFYYKDTYISLY
                     AGEPGTEVKIEWYIKADRIFFDFGEDDAKGELIPLRRLYEYEVLKEAPSDDYFYSPDG
                     IPYIPGVVIPDHVDLTKEASAEEINTDMESDFISLVKKVDEKPIPLSKYTSYTQFDTV
                     DKNGNTLTFVCTEFAYTTLQKNQATYVIWRNEQLTNIETGKTDIYKFVVGFENHYEIN
                     DNTFANGVTTRKLYVSEKKQVEGTFYKLSEGDYQYLNFLDNDGFLYSFFIGGQMDVES
                     LRNMAQGTKIKVSFYEKEIYIWEAGQRLLGRIAVDYTVIK"
     gene            1912..2313
                     /locus_tag="PKJ65_01445"
     CDS             1912..2313
                     /locus_tag="PKJ65_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNZ40534.1"
                     /translation="MKIDRIFVYGSLMKDFRFYKQYLEGKISNISYGKTHGTLYHLPE
                     GYPALVDGDGTVQGEIMEPVDGLLLADLDKFEGYTGTADNNLYIREKRTVLREDGKQQ
                     ECWVYIYANKKYAEEKGITVADGNWRRFISR"
     gene            2455..2724
                     /locus_tag="PKJ65_01450"
     CDS             2455..2724
                     /locus_tag="PKJ65_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNZ40535.1"
                     /translation="MIVIRPVSDLRNRFTEIEETVKEGKPVYLTKNGYGTMVVISLEQ
                     YTALTENIETKLDEADFMAAESTERYTAKEVFGRARKRIKEHEKI"
     gene            2711..3040
                     /locus_tag="PKJ65_01455"
     CDS             2711..3040
                     /locus_tag="PKJ65_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004011769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ40536.1"
                     /translation="MKKYRLRFLPLFEKDLNQIIDYISIQLENPVAAYSLIDEVYAAI
                     EKRLSCPESFEVFPSKKEHPLPYYRIYVRNYIIFYVISEDTMEVRRILYGRRDLEKHL
                     YIYRKHM"
     gene            3197..3925
                     /locus_tag="PKJ65_01460"
     CDS             3197..3925
                     /locus_tag="PKJ65_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ40537.1"
                     /translation="MRINNYISSAGICSRRKADELITQGKVKINGKTAVIGQVVNPGD
                     RVEVNGEYVKPSKNFVYIAFNKPVGVICTTERHVKNNIIDFINHPERIFPIGRLDRDS
                     EGLILLTGDGSIVNEILRDKNNYEKEYIVTVDKEITQDFIVKMSKGVRIFNPVKNTFT
                     RTKPCKVVKLDSYVFKIVLTQGLNRQIRRMCASFDYKVVKLRRIRIMDIGLKNLPPGK
                     WRNLTDDEVQSLLATRKSDDNQKI"
     gene            3906..4790
                     /locus_tag="PKJ65_01465"
     CDS             3906..4790
                     /locus_tag="PKJ65_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNZ40538.1"
                     /translation="MTTKKFKKIYIEITNVCNLNCSFCPPTKRKPEFMSIDTFGKILN
                     QIKPYTDYIYLHVKGEPLLHPDLSAILDISYEKGFFVNITTNGTLIPEIKDTLFAKPA
                     IRQINISLHSMENNNTDNTYIKNILTYIKQAVSDTSMIISLRLWNLDDKDSFSFGAGK
                     NSAVLQAIENAFNLPFRIEEASATDRGIKIADRIYVNQDFVFEWPDINAKEINTSGFC
                     YGLRNHVAILVDGTVVPCCLDQDGIMNLGNISETDFSKIIGSKRAIDIVNGFNNRQIT
                     EELCKKCPYISRFDIKSH"
     gene            4886..>6165
                     /locus_tag="PKJ65_01470"
     CDS             4886..>6165
                     /locus_tag="PKJ65_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40539.1"
                     /translation="MKKQVQLDEKGFRKFSIRDSIAYAAGDFGCNMSFALKGTMAIFW
                     TQFMQMDSILYAALLVIVQVWDAINDPLIGTIIDADRRQYKRNKFLTYIFVGSVGLIV
                     GGALCFIPWRNAPNIAKILIFVFGYVLWDAFYTIANVPYGSLLSLISADPADRASLSA
                     WRSVGAMAGNILPMVLLPAIIYDNNNNIVGVRVFIAALLMRVLGFIAFQFMIKNTVIR
                     VQEPVKLHEKQPKFNVFKALANFIRNRPAVGATVAAMGMFLGVQGATTAVTVLFQSYF
                     QNVKILGIVQLFALLPIFLFTPLARKMVVKYGKKELATVGSLCSLVSCILMIILPITP
                     DLKGLIIYIVCLFISSLGLGVYSTVSWAMMGDAIDYNEWKHGAREEGVTYSLHSFFRK
                     LAQGVGPAVALVIMVALGYVEANEGNQTFDVALR"
BASE COUNT         2074 a         1142 c         1266 g         1683 t
ORIGIN      
        1 gaatatgcaa ctgaaactgg aatttgtatc tacatgcaat gaaaacagtg aaccatataa
       61 taatgtaaaa gattaatcgg aaaaaccttc cgtcattgac tatatccttt ccgtttaagt
      121 caaactaaag agaaggaaat aacgcaatgc atggtataat atcaatgaca aaatgacatt
      181 tccgtggagg ttaagttaaa tgagatatat aaaccgcgta tttcaatcgt caggacaaat
      241 tgatgtgcaa tccgtaaaga ataaaagttt gcgtcttgca attcttttca tggtccttct
      301 gctcctgctt acatcgtgcg gacctgcggt aacaccccgc ccttccgggg tagctactct
      361 taccccgact acaccttcgg aaacacctgc tccttcggaa acgggtacac cggttgttga
      421 agaccctgta ccttcggaag aaccgttacc ccaaccgaaa accatggaaa ttgacggtgt
      481 aacatatgag atggctcccc tgcccgaatt tccggagccg gaggaattgg atttcgctgc
      541 ggagaaaaag aaatatcaag aggacgaaga ttattcggca aactatctct ccactgtaag
      601 acttatttca tataaagaag ataaggtttt agggtatcgt acggaagtaa cggcaaggaa
      661 tgcaaacaaa gaaactctta aattttatcc taccgagtac cagttttata acttcttcaa
      721 agagtatatg ggtgagattg tcaatgtaat ctggtacttc gacggcaatg cttatgatga
      781 gaacgacaag gaactttact gttgtgcgta cgatatgtat atcgatgaag aaaccaatca
      841 gaacgataac tttgttgaag gcaaactcac atcgtacaag ttgaacagcg attatgatat
      901 cagaataaca tcgggtgtcg taaaactctc cgaaagaatt gatgatcttt tcttagttct
      961 atatgtactg ggtgaggacg gttatctgta cgacttttat tacaaagata cttacatatc
     1021 gctttatgca ggagagccgg gtaccgaagt gaaaatagaa tggtatataa aagccgatcg
     1081 tatatttttt gatttcggtg aggatgatgc aaaaggcgag cttattcctt taagacggct
     1141 ctatgagtat gaggtattaa aagaagcccc ttcagacgat tatttctata gcccggacgg
     1201 cataccgtat atccccggtg ttgtcattcc cgaccatgtg gatttgacaa aggaagcaag
     1261 tgcggaggaa ataaataccg acatggagtc ggattttatc tcattggtga aaaaagtgga
     1321 tgaaaaacct atccctctaa gtaaatacac atcatatact caattcgata ccgtagataa
     1381 aaacggtaac accctgacct ttgtttgcac cgaatttgct tacactaccc tgcagaaaaa
     1441 tcaggctacc tatgtcatct ggcgaaacga gcaattaacc aacattgaaa cgggaaaaac
     1501 cgatatatat aagtttgtag taggttttga gaaccattat gaaataaatg acaacacttt
     1561 tgcaaacggg gttaccacaa ggaaacttta tgtgagcgag aaaaagcaag tggaaggcac
     1621 cttctataaa ctgtccgaag gagactacca atacttgaat ttcctggata atgacggatt
     1681 cctgtattcc tttttcatcg gaggtcagat ggatgttgaa agtttgagaa atatggcaca
     1741 aggaaccaaa attaaagtat cattctacga aaaagagatc tacatatggg aagccggtca
     1801 aagacttttg ggcagaatag cggtggatta tacggtcatt aaatagaacg atattccgaa
     1861 aatcctccat cccctttacg gaagatacgg atattcagta aggcggtgca tatgaaaata
     1921 gacagaatat ttgtatacgg atcattgatg aaagatttta ggttctataa gcaatatctt
     1981 gaaggaaaga taagtaatat aagttatgga aaaacacacg gaaccctcta tcatcttccg
     2041 gaaggttatc ccgctttagt agacggagac ggaacagtac aaggcgagat tatggaaccg
     2101 gtcgatggtt tacttttggc tgacctggat aaatttgagg gttacaccgg tacggcggat
     2161 aataatttat atatcagaga aaaaagaacc gttttgagag aagacggaaa acaacaggaa
     2221 tgttgggtat acatctacgc taacaaaaaa tatgccgaag aaaaagggat aacggtggcg
     2281 gacggaaact ggagaagatt tatatcaaga tagttattta agcactcaag tcaataaata
     2341 tgcttatact ttacgtatat tacaataatg tgattaaaat cgtatttaaa catttctctt
     2401 gccaatatgg cgcaattatg ataatattac gccatcagaa atgaggtgat tttaatgata
     2461 gttatacgcc ctgtatcaga tttacgtaac cgtttcacgg aaattgaaga aaccgttaaa
     2521 gaaggtaagc cggtttattt aacaaaaaac ggctatggaa ccatggtcgt tattagttta
     2581 gagcagtaca ctgccctaac ggaaaacatt gaaactaaac tggatgaagc cgatttcatg
     2641 gctgcggaat ctacagaacg atatactgca aaagaagtgt ttggcagagc aagaaaacgc
     2701 attaaggaac atgaaaaaat ataggctacg atttcttccc ctctttgaaa aagatttaaa
     2761 tcaaattatc gattacataa gtatccaatt agaaaatccg gttgctgcat attcattaat
     2821 agacgaggta tatgcagcaa tagaaaaacg cctttcctgt ccggagagtt ttgaagtctt
     2881 tccttcaaaa aaagaacatc ctctacctta ctatagaatt tatgtaagaa actatattat
     2941 tttttatgta atttctgaag acaccatgga agtaagacgt atcttatatg gccgtcgtga
     3001 tttggaaaaa cacctgtata tttatcggaa gcatatgtag tagtcacttc ttataccgcc
     3061 caagttattt gaaataaata tcttctatga ccgaaataac tttgcactca agaccgaaat
     3121 aagtttttgt aataacactg aaacaattct gatataattc tatactacaa tacgattgct
     3181 ttatgaggaa taaccaatga gaataaacaa ctacatcagt tccgcaggta tatgctccag
     3241 aaggaaggcg gatgaactta tcacacaagg taaagtcaag ataaacggta aaactgctgt
     3301 tataggtcag gttgtcaacc ccggcgacag agtggaagtt aacggtgaat atgtaaaacc
     3361 ttctaaaaac ttcgtgtata tagcatttaa caaacccgtc ggagttatat gtaccaccga
     3421 aagacatgta aaaaacaaca taattgactt catcaatcat ccggaaagga tatttcccat
     3481 agggcgactg gacagagatt ccgaaggttt gattcttctt accggtgacg gtagcatagt
     3541 aaacgaaatt ttaagagaca agaacaacta tgagaaagaa tatatagtaa cggtggataa
     3601 agagataacg caggatttta tcgtaaagat gtccaaagga gtgaggatat tcaacccggt
     3661 aaaaaacacc tttacccgca caaaaccctg caaggtagtg aaactggatt cttatgtgtt
     3721 caagattgtt cttacgcagg gattaaacag acagataaga agaatgtgtg cttcttttga
     3781 ctataaagta gtaaaactca gacgtatacg cataatggat atagggttaa agaatcttcc
     3841 tcccggaaag tggagaaacc ttacggacga tgaagtgcaa tctttacttg caacaagaaa
     3901 atccgatgac aaccaaaaaa tttaaaaaaa tatatataga gataaccaat gtatgcaatc
     3961 tgaattgctc cttctgcccg cccaccaaac gcaagccgga gtttatgagt atcgatactt
     4021 tcggtaagat attaaatcag ataaaacctt ataccgacta catatatctt catgtcaaag
     4081 gagagcctct tctccacccc gatttaagtg ctattctgga tatcagctat gaaaaagggt
     4141 tttttgtgaa tataacgaca aacggcacac tgataccgga aattaaagat accttatttg
     4201 ctaaacccgc aataaggcag ataaacattt cattgcacag tatggagaat aataatacgg
     4261 ataataccta cattaagaat atattaactt atatcaaaca agctgtcagt gacacgagta
     4321 tgattatttc tttaaggctg tggaatcttg atgataagga ttcctttagt ttcggtgcag
     4381 gaaaaaactc tgccgtatta caagcaattg aaaatgcatt caacctgcct ttcaggattg
     4441 aagaagcatc ggcaaccgac cgcggtatta aaatagccga cagaatatat gtcaatcagg
     4501 attttgtctt tgagtggccg gatataaatg caaaagaaat aaacacatcg ggtttttgtt
     4561 acggactgcg caatcatgtt gccatattgg ttgacggaac ggttgtcccc tgctgtctgg
     4621 atcaggacgg gataatgaac ttgggcaata tttcggaaac agatttttct aagattatcg
     4681 gcagtaaaag agcaattgac atagttaacg gttttaacaa caggcagatt acggaagaac
     4741 tttgtaaaaa atgcccatac ataagcagat tcgatatcaa atcgcattaa aaataatgac
     4801 atattcagcc tttttatagt aaaataaaat aagaagttgt aaaacaaaat aaacctttca
     4861 tcaaacaaat aaggaggaaa ttcccgtgaa gaaacaagtt cagttagatg agaaagggtt
     4921 caggaagttc agcattcgcg acagtattgc ttatgcagca ggtgatttcg gctgcaatat
     4981 gagttttgcc ttaaaaggca ccatggcgat tttctggaca caattcatgc agatggattc
     5041 catattgtat gcagcactgc ttgtaatagt gcaggtatgg gatgcaatca atgacccctt
     5101 aatcggaacc attatcgatg ccgacagacg tcagtataag agaaataaat ttctcacata
     5161 catttttgta ggctccgtgg ggcttatcgt aggaggagcg ctgtgcttta tcccgtggag
     5221 aaatgcaccc aatatagcaa aaatactcat attcgtattc gggtatgtcc tttgggatgc
     5281 tttctatacc atagcaaacg taccgtacgg ttcgcttctt tcactcatat ccgccgaccc
     5341 cgccgacagg gcttccttat cggcatggcg ttcggtagga gcaatggccg gcaacatact
     5401 gcctatggtt ttattaccgg ctatcattta cgataacaat aataacatcg tcggagttcg
     5461 tgtattcata gccgctcttc tcatgagagt attgggattc atagcattcc agtttatgat
     5521 taaaaatacc gtaatacgtg tacaggaacc ggttaaactt catgaaaaac agcccaaatt
     5581 caatgtgttc aaagcactgg ccaatttcat acgtaaccgt cccgctgtag gtgcaaccgt
     5641 tgccgcaatg ggaatgtttt taggcgtcca aggtgcaact accgcagtta ccgttctttt
     5701 ccagtcatat tttcagaatg taaagatact gggaatagta cagctttttg ccttactccc
     5761 catattcctt tttacacctt tagccagaaa aatggtagta aagtacggaa aaaaagaact
     5821 ggcaacggta ggttctcttt gcagccttgt atcatgtatc ttaatgatta tcctgcccat
     5881 tactcccgac ttaaagggtc tcataatcta tatcgtatgc ctgttcataa gcagcttagg
     5941 cttgggagta tattccaccg tttcctgggc aatgatgggt gacgcaatag actacaatga
     6001 atggaagcac ggcgccagag aagaaggcgt tacctactcg ttacattcat tcttcagaaa
     6061 acttgcacag ggagtcggac ctgccgtagc tcttgtaata atggtggcgc tgggatatgt
     6121 agaagccaat gaaggcaatc agacatttga tgtagcctta agaat
//
LOCUS       DAOPWC010000036         6159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_12683_length_6159_cov_3.401376, whole genome shotgun sequence.
ACCESSION   DAOPWC010000036 DAOPWC010000000
VERSION     DAOPWC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_6159_cov_3.401376"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1733)
                     /locus_tag="PKJ65_01475"
     CDS             complement(381..1733)
                     /locus_tag="PKJ65_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNZ40540.1"
                     /translation="MKKSMAVLLILIVLSLTACNIGSKPAKECREGSFIYIVKDNEVT
                     IIKYDGTEETVIIPDRISNKPVACIKDKAFYACSHVKEVFIPESVKRVGVCAFDDTEW
                     YKSLKDEFVVVGDGVLIKCNQTSKDIVIPEGVKSLSSTFYKAGDGGFFDAVIYLPATL
                     KKIDDYAFYKAFGHIIVCNTEQIEEIGQSAFEDSTVDRIIISDKLKSIKDRAFATTDK
                     TVKHSMTIEMTGGGYYVGTQKKVKLPFATSLGKDIFYGLDGNMETVKFEDGTTAITKD
                     MLTCLQSFAKFFIPGEVTCIEKDIFPEGAIIYTEAGSAAEKYAKDNSVKVLILPESFK
                     DRALEKLLIPAFLSADLEFTYDNMQIITSIQIRDQVEGNVYDHYRVYVIVQAATGAKG
                     IEHILKQDNVKSLEDLKFFTKLEYLLLESAIIDGSVDCAVLKSMPALKEAVVNNESLK
                     "
     gene            complement(2480..3124)
                     /locus_tag="PKJ65_01480"
     CDS             complement(2480..3124)
                     /locus_tag="PKJ65_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNZ40541.1"
                     /translation="MKSVLIIGTGRFGKSLALKMAELGNEVMVVDKDESELRDLSEHV
                     TSALVADCMDENVLKSFGINNFDICFVCIGENFQSSLEITSLLKDNGAKYVVSKADRD
                     IHAKFLLRNGADEIIFPEKDMANRAAYRFSANHMFDYIQLSPDYAVSEIKTPEQWIGK
                     TISHVNVRMNFNINVLAYKEDGKIHPLSNPNYIFKEHTRLIVSGAWKDVEKISK"
     gene            complement(3134..4477)
                     /locus_tag="PKJ65_01485"
     CDS             complement(3134..4477)
                     /locus_tag="PKJ65_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNZ40542.1"
                     /translation="MEAKKKIRLTTMQIIAIGFVVIILIGALLLSLPAASKDNTRIPF
                     INALFTATSALCVTGLATYDTYTQFTLFGQIVILVLIQTGGLGFMSMAGMIFLMLGKK
                     IGLRERGLLMETMGSPQIGGVVKLLRKIIITSFAIEIVAATILSIRFIPLLGTAKGIY
                     YGIFHAISAFCNAGFDLMGVFSPRSSLTFFDTDIVVNIVIMSLIVVGGLGFVVWDDIM
                     DNKLKFSKYRLHTKIVLIGTAVLITVPAVIFFFLESNNTLEGMTFGEKILVSLFQVIT
                     PRTAGFNTVDMTELSEGASFLTVLLMMIGANPGSTGGGIKITTMFVIILTIKAYITHE
                     PDLNVGGKRLEDFIPRKAFSIAAIYLSLTLLACFLIISFQSISVKDAMFESFSAIGTV
                     GLSTGITPELNILSKIAVIILMYCGRVGSLTVIAALAGDKPRPNVRLMEERIIIG"
     gene            complement(4481..5545)
                     /locus_tag="PKJ65_01490"
     CDS             complement(4481..5545)
                     /locus_tag="PKJ65_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ40543.1"
                     /translation="MQKAKTYYKGLKPYTNLDDKDLIYLDVREQPFSLYGLYDPANQP
                     VFKRMPDEVAAKCNSRVRIHYLHTSGGRIRFCTDSDYLCIQVFFPELFRMTNMSLQAA
                     SGFDIYEDFDNRSVFFSRFNPEVNTKDWYEMRIRFSEHKLRYFTLYFPLYNNMDKVYI
                     GVSEDSLLGPGKQYKYKKPVVYYGSSITQGGCASRAGNCYEAVISRMCDVDYINLGFS
                     GAAEGEQAIAEYIAGLDMSVFVLDYDHNAPKIEHLQKTHQPFFNTVRKKNPDLPVIFV
                     SKPDFSDYSFDSIQRRNIIFKTYMSAMENGDRNVYFIDGRSFFARDYRDSCTVDGCHP
                     NDIGFLRMAQVIGNMVKELL"
     gene            complement(5568..>6159)
                     /locus_tag="PKJ65_01495"
     CDS             complement(5568..>6159)
                     /locus_tag="PKJ65_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40544.1"
                     /translation="MVYDTVKLKLYENIVRILLTDKYPDDFKNGVNEQTMALIRNSYK
                     DRLHKDLPVGFVTNMSLVGIGAPTHIFLPDVAKALGTGCVIPSDAKYANAIGAVVSNV
                     RSVYQIEIRSEYDSSYMVYAPSGRYVTNDFDEAVEYAKKAASEAALKDAQSKGIADNY
                     NIDTQVITNTATAKKGEQINLGTTVIATVSGNMQYN"
BASE COUNT         1707 a         1375 c         1058 g         2019 t
ORIGIN      
        1 aagcgtcaaa agtacagaca attagccttc agttttttat cctgagctat aaaaaattga
       61 atactcatga aaagttccga catagtggga actgattttt atacgcaaaa ctccggtttt
      121 agagactttt atatgcaatt ttactaatct ttcctctata gtcttaaagc tacctcaaaa
      181 accggtcaat tatccggttt cgagtgcatt ttacggaaac tcaaggttcc gcgtatcgtc
      241 cttatcccgc cgggcaatgt ttattatgct tttacgcgca taaaaagaca gaggacgact
      301 gctctttaca tatatttctc tttttcatcc taaaaacaga ctgcttttat aactgcttcg
      361 gcaatcgcct tccgcaatca ttattttaat gattcgttat tcaccaccgc ttctttaaga
      421 gcgggcatac ttttcagaac ggcacaatcg acggacccgt cgataatcgc ggattcgagt
      481 aacaaatatt ccagtttggt gaaaaactta agatcctcaa gactctttac attatcttgc
      541 ttcagtatgt gttctatgcc tttggcgccg gttgcagctt gcactatcac atagacacgg
      601 taatgatcat aaacattgcc ctccacttgg tcgcgaatct gaatcgacgt aattatctgc
      661 atattgtcat atgtaaactc aagatcggcc gaaagaaaag ccggaataag cagtttttcc
      721 aatgccctgt ccttaaaact ctccggcaaa atgagtactt tgacagaatt atctttagca
      781 tatttttcag cggcgcttcc ggcttcggta tatattatcg ctccttcggg aaatatgtcc
      841 ttttcgatac atgtcacttc ccctggaatg aagaattttg caaacgactg caaacaggtc
      901 agcatgtctt tggttatcgc agtggtcccg tcttcgaatt taactgtttc catgttgccg
      961 tcgagtccat aaaaaatatc ttttcccaat gatgttgcaa acggaagttt aacctttttt
     1021 tgcgtaccaa cataatatcc gccgcctgtc atttcaatcg tcattgaatg ttttaccgtt
     1081 ttatcggttg ttgcgaaagc cctgtccttg atgcttttca gtttatcgga tattatgata
     1141 cggtctacgg tgctgtcttc aaacgcactt tgacctatct cctctatctg ctccgtatta
     1201 catacaatga tatgtccgaa cgctttatag aatgcgtaat catctatctt tttcaatgtg
     1261 gcgggcaggt atatgacggc atcgaagaaa cctccgtccc ctgccttgta aaatgttgaa
     1321 gaaagacttt tcacgccttc cggtatcact atatctttac tggtctgatt acatttaatc
     1381 agtaccccgt cgcctacgac aacaaattca tccttgagac tcttgtacca ttcagtatca
     1441 tcaaaggcac agactcctac tctctttacg ctctctggta tgaatacttc ttttacgtgc
     1501 gaacatgcgt aaaaagcctt gtctttgata catgctacag gcttattgga aattctgtca
     1561 ggtattatca ctgtttcttc cgtgccgtca tattttatga ttgtcacttc attgtctttt
     1621 acgatgtaaa taaaggatcc ttctctacac tcttttgcag gtttgcttcc gatattgcag
     1681 gccgtgagcg acaaaactat taaaatcagc aataccgcca tgcttttttt catgtcattt
     1741 gtttcctttc cttttcatac ttgagcgcaa atcatcgtat atacatttct tggctgatat
     1801 actccggggt tactctgcca gtcgcctgtt ctgatacatt tcctaacatt attttctttg
     1861 gtcactatct gcgttgaatc tccgctgtct accgaaatcc catgagaaat ttgagtaccc
     1921 caaatatttt taatcattgc tctatgctca taaagactca gtccggatgt tattggtgac
     1981 aaatccgagt tggtactgaa tacccaaagc aatacaatat cttcatatgc attattgagt
     2041 ttgaatattg cacttctgtg agttattgaa agatggactc catttgcttc gtctgtaact
     2101 aaattgttat attgtgtttc tgataaaatt tgtcccatgt ataaattcac tccacctatg
     2161 gcatattgga tttgtgattg ggttatggat tcgttaagat atgagtaatt gttaaaactt
     2221 gttaatgaat ccgcataaat atatcgtcat gatgtatttc tatagcctca taataaacac
     2281 ttctacccgc atttggtacc gaatagaaat cccttctata attagccatt taatacctct
     2341 ccgtttctac cttacgagta tggcatatta cgtttcattt gtcaacggag tcgtgacacg
     2401 gagtcgtacc ttgatacttt actacagata tgacaatatt agatgcagtg ccctcactca
     2461 tcgcttcgta ccacaaatcc tacttactta tcttctcaac atccttccac gcaccggata
     2521 ctataagccg tgtatgctct ttgaagatat agttggggtt ggaaagggga tgtattttac
     2581 cgtcctcttt gtatgcaagc acgttgatgt taaagttcat tcttacatta acatgggata
     2641 ttgtctttcc tatccattgc tcgggtgttt ttatctccga taccgcataa tcgggtgaaa
     2701 gctgtatata gtcaaacatg tgatttgcac tgaatctgta tgccgccctg tttgccatat
     2761 ccttttccgg aaatataatc tcgtcagcac cgtttcttaa aagaaatttt gcatgaatat
     2821 ctctgtcggc tttggacacc acatatttgg ctccgttatc ctttagtaat gatgttattt
     2881 ccaaagaaga ctggaaattc tcccctatgc atacaaaaca tatatcaaag ttatttatcc
     2941 cgaatgattt taatacattc tcatccatgc aatccgccac caatgcggaa gtaacatgct
     3001 cggatagatc tctgagttca ctttcatcct tatccactac cattacttca tttcccagct
     3061 ccgccatttt taatgccaag ctttttccga atctgcctgt tcctattatt aaaaccgatt
     3121 tcattttttg ctcctaacct attattatcc tttcttccat cagccgcaca ttaggcctgg
     3181 gtttatcacc ggccaaagcc gctataacgg tcaagcttcc caccctgccg cagtacatta
     3241 atattataac cgcaattttt gataatatgt tcaattccgg agtaataccc gttgataacc
     3301 ctacagtacc gattgccgaa aacgactcaa acatagcatc tttcacggaa atggactgaa
     3361 aggatattat caaaaaacaa gccaaaagag taagtgataa ataaatggca gctatgctga
     3421 aggcttttct cgggataaag tcttccaacc ttttccctcc cacattcaaa tccggctcgt
     3481 gagttatata tgcttttata gtaagaataa taacaaacat tgtagttatc tttattccgc
     3541 ctccggtgga accgggattg gcacctatca tcatcagaag aactgttaag aaagaggctc
     3601 cttccgacag ttccgtcata tctacggtat tgaatccggc ggttctggga gtgataacct
     3661 gaaacaggga aactaaaatc ttctctccga aggtcatacc ttccaaagta ttattgcttt
     3721 ccagaaagaa aaaaatcact gccggaacag taatcagtac tgcggtacct ataagcacta
     3781 tctttgtgtg cagtctgtat ttggaaaatt tcaatttatt atccataata tcatcccaca
     3841 ctacaaagcc gagaccgccg actactatca aagacattat aactatattg acaacaatgt
     3901 ctgtatcaaa aaaggttaat gaacttctgg ggctgaatac acccattaag tcaaatccgg
     3961 cattacaaaa tgcggatatg gcatggaata tgccgtaata tattcccttt gccgtcccta
     4021 aaagtggtat aaatctgatg gagagaatag ttgccgcaac aatttcaatt gcaaaggatg
     4081 taatgattat cttacgcaag agtttcacaa ccccgcctat ttgcggagaa cccatggtct
     4141 ccatcagaag ccctctttct ctcaaaccta tctttttgcc caacatcaga aatatcatac
     4201 ccgccatact catgaaaccc aggccgcctg tctgtataag taccaagatt acaatctgac
     4261 cgaacaatgt aaactgagta tatgtatcgt atgttgcaag tcctgtgaca cacaatgcag
     4321 aggtggcggt aaacagggcg tttataaaag ggattcttgt gttgtctttt gaagcggcgg
     4381 gaagagaaag cagcaatgct ccgattagta ttattacaac aaatcctatt gcaataatct
     4441 gcatggttgt taatcttatt ttcttcttcg cttccattag ttataaaagc tccttaacca
     4501 tatttcctat tacctgtgcc atacgtaaaa aacctatatc gttagggtga caaccgtcta
     4561 ccgtacaact gtccctgtaa tctcttgcga aaaaacttcg gccgtctatg aaatacacat
     4621 ttctgtcgcc gttttccatt gcactcatat atgtcttaaa gattatattc cttctctgta
     4681 tgctgtcaaa tgagtaatcg gaaaagtcgg gtttggaaac aaatattacc ggaaggtcgg
     4741 gattcttctt tcttacagtg ttaaaaaacg gttgatgtgt tttttgtaaa tgctcaatct
     4801 tcggagcatt atggtcataa tccagcacaa atacgctcat gtccaatccc gctatatatt
     4861 ccgctattgc ctgttctccc tcggcggcac cggaaaagcc taagtttata taatcgacat
     4921 cgcacattct ggatatgacg gcttcataac aattgcccgc tcttgaagcg catcctccct
     4981 gtgtgatgga ggaaccgtaa tacacaacag gcttcttata cttatattgc tttccgggac
     5041 ctaagagaga atcttccgaa acccctatat ataccttatc catattattg tataaaggga
     5101 aatacaaagt aaagtatcgc aatttatgct cactgaatct tattctcatc tcataccagt
     5161 ctttagtgtt tacttcggga ttaaatctgc tgaaaaatac acttctgttg tcaaaatctt
     5221 catagatatc aaatcccgag gcggcctgca aagacatatt ggtcatcctg aataattcgg
     5281 gaaagaagac ctgtatgcaa agataatcgg aatcggtaca aaaccgtatt cttcctccgg
     5341 aggtatgtaa atagtgtata cgcactctcg aattacactt agcagccact tcatcgggca
     5401 ttcttttaaa taccggttga tttgcggggt catatagtcc atagagactg aagggttgtt
     5461 ctctcacatc aagatatatt aaatctttat catctaaatt ggtgtacggc tttaagcctt
     5521 tgtaatatgt ttttgctttt tgcatatctg ttaatccttc tttcttttta attatactgc
     5581 atattcccgc ttaccgttgc aataactgtt gtcccaagat ttatttgttc tccttttttt
     5641 gccgttgcgg tatttgttat cacctgtgta tcaatattat agttgtcggc aatacctttg
     5701 gattgtgcat cttttaaagc cgcttccgat gccgctttct ttgcatattc caccgcttca
     5761 tcaaaatcat ttgtcacata cctgcctgaa ggagcatata ccatatatga actgtcatac
     5821 tcgcttctaa tctctatttg atataccgac cttacattgc ttacaaccgc tcctatcgca
     5881 ttggcatatt ttgcatcgga aggtattaca catcctgtac ccaaagcctt tgcgacatcg
     5941 ggaaggaata tatgtgtagg tgcccctata ccgactaaag acatatttgt tacaaaacca
     6001 acaggcaggt ctttatgtaa acggtcttta tatgaattgc gtataagggc cattgtctgc
     6061 tcatttaccc cgtttttaaa atcatcggga tatttatcgg ttaacagtat tcttacgata
     6121 ttttcgtaca gtttaagctt tacggtgtca tataccata
//
LOCUS       DAOPWC010000037         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_13635_length_5839_cov_4.313624, whole genome shotgun sequence.
ACCESSION   DAOPWC010000037 DAOPWC010000000
VERSION     DAOPWC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13635_length_5839_cov_4.313624"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="deoC"
                     /locus_tag="PKJ65_01500"
     CDS             complement(<1..548)
                     /gene="deoC"
                     /locus_tag="PKJ65_01500"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNZ40545.1"
                     /translation="MNIENILSKVDHTLLKQTAKWEDIKAICEDAIKYNCATACIPPY
                     FVKQAKDCFKNNLKICTVIGFPNGYSTTKAKCFEAAQAVSDGADEIDMVLNINMLKDK
                     LYDDILAEINQVKKACSGKLLKVIIETCLLTEEEKIKMCEIISKSDADYIKTSTGFST
                     AGATREDIMLFKEHIKNGKLIKA"
     gene            693..1823
                     /locus_tag="PKJ65_01505"
     CDS             693..1823
                     /locus_tag="PKJ65_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ40546.1"
                     /translation="MADIFSNVLEIRWEMVLMWVIGGVLIWLAIAKDMEPTLLLPMGF
                     GAILANLPTSGAVTQIINGNTEEGVLDVLFNAGIANELFPLLLFIGIGAMIDFGPLLS
                     NPKMLLFGAAAQFGIFFTFSLAALLGFDLKDAASIGIIGAADGPTSIFVANYFNSNYI
                     GAIIVAAYSYMALVPIIQPPVIRLFTTKKERLIRMPYEQKEVSATVRILFPIVTTMIV
                     GILAPRSVALVGFLMFGNLLRECKVLRILSESAQKELANLVTILLGITIASKMRYDLF
                     VKVDTLVIILLGLVAFVFDTIGGVLFAKLLNLFVKKKINPMVGAAGISAFPMSSRVVH
                     KMGLKEDPTNFLLMHAAGANVAGQIASVVAGGVMIALVEKLI"
     gene            1836..2003
                     /locus_tag="PKJ65_01510"
     CDS             1836..2003
                     /locus_tag="PKJ65_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNZ40547.1"
                     /translation="MNIRFDVDNFLQTPGVVLFGMLGVFIALTVIYLAILLLSRIFKD
                     NKKKDSGATEK"
     gene            complement(2066..3052)
                     /locus_tag="PKJ65_01515"
     CDS             complement(2066..3052)
                     /locus_tag="PKJ65_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ40548.1"
                     /translation="MKKIAIFITLISVLVCSAVYAADIPAPTSEFYVNDTANVLSEKT
                     ERTIIDKNIALYEKTGAQIVVVTVDYTHTASIADYAYELFNEYKIGSAEKNNGLLLLL
                     SIGDDDYYVLQGEGLERTLSSGTLSYLLYEHLEPYFAKQQYDEGVLSIFNVLYKEMLA
                     IHNTTIEYEDVYPPQDNYYYPEDFYNPSPPLTFFDILVTVFAVFRFFFFFIVIIGVLS
                     AIFGRRKSTRRSDVARNVMPVIGALGYMNTLKNMNNSTNYSSFNNNSSTVNRSNYYSG
                     GSSYSSGRSSYSGGSSFRSSSSSSRSSFSSGSRSSSGGGGSSRGGGAGRGGR"
     gene            complement(3083..3610)
                     /locus_tag="PKJ65_01520"
     CDS             complement(3083..3610)
                     /locus_tag="PKJ65_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ40549.1"
                     /translation="MRFKENRALAWILVVIAVIASVLISGHVSLSLQRRAVMNSFYDT
                     MDADLNTKSAYADNLAGVASRYLDRNSEYITNMAQARDMLLNAKTPAEKYAASVKITN
                     AAATLYDILGTMSLNETDERLRRSNYADIIAVDDILKRTSFNKNVDTFNSQLALFPAN
                     VIASITGIDKAEYFR"
     gene            complement(3610..4644)
                     /locus_tag="PKJ65_01525"
     CDS             complement(3610..4644)
                     /locus_tag="PKJ65_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNZ40550.1"
                     /translation="MNESTLTEKDRELAISYGDKLDIKSSASILQYGADAQRKITTFS
                     DATLEKIRSKELGEIGDMIGNLMAQLKDFTADQESGNFLERLFKRTKRYIDVLKARYA
                     KVEANVNKIVAVMEDHQVVLMKDITMLDKMYEMNLDYVKELALYIEGGRAKHKELKET
                     KLVELRKKAKETNLPEDAQAANDFANMLDRVEKKIYDLEITRNIAVQMAPQIRLIQNN
                     NTLLTEKIQTTLINTIPLWKNRMVLSLSITHSQNAMKAQRAVTDMTNELLLKNAEMLK
                     QSSVDIAKESERGIVDIETLKATNESLITTLEEIIRIQEEGKAKRREAEKELDRIEEQ
                     LKNKLLEFKN"
     gene            complement(4662..5279)
                     /locus_tag="PKJ65_01530"
     CDS             complement(4662..5279)
                     /locus_tag="PKJ65_01530"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HNZ40551.1"
                     /translation="MEVRHKSAIPIYIAGAVWVLYSIIFPMHKLIHIIIAAILFILAF
                     FLSGRFFPDRIEMVEEPIKYTGAKDADMAIAEGNRYIEKMRKANIRIQNAVVSEKIDI
                     LSEVIKKIFAYITEKPEKVKDIRRLITYFLPTTVKLLNAYARAEEQNDDGENITQVKT
                     DIEGAMDGIIHSFRKYLDNLYADEAMDIDAEITVFDSMLKAENLK"
     gene            5469..>5839
                     /locus_tag="PKJ65_01535"
     CDS             5469..>5839
                     /locus_tag="PKJ65_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNZ40552.1"
                     /translation="MMLTENAKKVLEKRYLKRDSEGNVVESYDDMFRRVAKTVASADA
                     EYLGKEDIEKSEKDFYDMMIKLEFLPNSPTLMNAGRPLGQLSACFVLPIEDSMDGIFE
                     TVKNAALIHKSGGGTGFSFSR"
BASE COUNT         1592 a         1200 c         1201 g         1846 t
ORIGIN      
        1 gcttttatga gcttgccgtt tttaatatgt tccttaaaaa gcataatgtc ttctctcgtg
       61 gcacctgctg tggaaaagcc cgttgatgtc tttatatagt cggcatcgct ttttgaaatg
      121 atttcgcaca tctttatctt ttcctcctcg gtcagaagac aggtttcaat tataacttta
      181 agaagttttc ccgaacaggc tttttttacc tgattaattt cagccagtat atcatcgtaa
      241 agcttgtctt tgagcatgtt tatgttgaga accatatcta tttcgtcagc tccgtcgctt
      301 acagcctgtg cggcttcaaa acatttggcc tttgttgtac tgtatccgtt gggaaaacct
      361 ataaccgtac agattttaag gttattctta aaacaatctt ttgcctgctt aacaaagtac
      421 ggaggaatac aggccgtcgc acaattatat ttaatcgcat cttcacaaat cgctttaata
      481 tcttcccact tcgccgtctg cttgagaagg gtatgatcta ctttggataa tatgttttca
      541 atattcattt tataattcct ttcccagtaa agtatatata tacaaacggt ttaatgcaac
      601 taatcacttg acgcaagcgg caggaaaaaa gtattatata tactaaagat gaacaggcaa
      661 taagcctgta aaaatcgggg gacaagacaa aaatggctga tattttttct aatgtactgg
      721 agataagatg ggagatggtc ctcatgtggg tgataggagg ggttttgatt tggctggcta
      781 tagccaaaga catggaaccc acactgcttc tgcctatggg gttcggggca attcttgcca
      841 atcttcccac atcgggagcg gtaacgcaga ttattaacgg caatacggaa gaaggtgttc
      901 tggatgtgct tttcaatgcg ggaatagcta acgagttgtt tccgttgctt ctgtttatcg
      961 gaataggagc aatgatagat ttcggaccgc ttttatccaa tcctaagatg ctgctgttcg
     1021 gtgcggctgc acagttcggt atattcttca ccttttcctt ggctgctttg ttgggctttg
     1081 atttgaaaga tgccgcatca ataggcatta tcggagctgc ggacggtccc acatccatat
     1141 tcgtagccaa ctacttcaac agcaactaca taggagcgat tatagtggcg gcgtattcgt
     1201 atatggcatt ggttcctata atacagcctc ccgttattcg tcttttcaca accaaaaagg
     1261 agcgacttat aaggatgcct tacgagcaga aggaagtatc cgcaacggta agaatactgt
     1321 tcccaatagt aaccacaatg atagtaggta ttctggcacc gagatcggta gcactggtag
     1381 gattcttaat gttcggtaac ctgttaagag aatgcaaagt cttgaggata ttatccgaat
     1441 cggctcaaaa agaacttgcc aacctcgtaa ccatattgtt gggaataacc atagcttcca
     1501 agatgagata tgatcttttc gtaaaggtgg acaccctggt gattattctt ttgggacttg
     1561 ttgcattcgt gtttgacacg atagggggag tgctgtttgc caaacttctg aatctgtttg
     1621 tcaagaagaa aataaatcct atggtaggtg cggcgggaat atcggcattc cccatgtcct
     1681 ccagggtagt acataaaatg ggattaaaag aagatcctac aaacttcctg ttaatgcatg
     1741 ctgccggagc caatgtagcc ggtcagattg catcggtggt tgccggcggc gtaatgattg
     1801 ccctggtgga aaaacttata taacggaggc gaaaaatgaa tatacgattt gatgttgata
     1861 attttttaca gacacccggg gtggttcttt tcggaatgtt aggtgttttt atagcactta
     1921 cggttatata tcttgcaata ctgctattgt caagaatctt caaagacaat aagaaaaagg
     1981 actcgggcgc aacggaaaag taaaagtcaa tacacgattg atatgaaatt aaaacaggtt
     2041 tgatatagag gaaataacag ggcggctatc tgccgcctct tcccgctcct ccgcctctgg
     2101 acgagccgcc tccgccgctg ctggatctgc taccggaaga aaaagagctt cttgaagaag
     2161 aggaactgct tcggaaagag gatcctcctg aatacgaact tctgcctgac gaataactgc
     2221 ttccgcccga atagtaatta ctcctgttga cggtactgct gttattgttg aatgaagaat
     2281 agtttgtgga attgttcata tttttcaatg tattcatata acccagagca cctataacgg
     2341 gcattacatt tcgggcaaca tcggaacgcc ttgtactttt ccttctcccg aatattgcgg
     2401 aaagtacgcc gataataact atgaaaaaaa agaaaaatct gaaaacggca aaaacagtta
     2461 cgagtatatc aaagaaagtc aatggagggg acggattata gaaatcttcc ggatagtaat
     2521 aattgtcttg cggcgggtat acatcttcat attctatggt tgtattgtga attgccagca
     2581 tctctttgta taaaacatta aaaatgctta atactccctc atcatattgt tgcttggcga
     2641 aatacggttc cagatgttca tatagcaggt aagaaagagt accgctggac agcgttcttt
     2701 ccaagccttc cccttgcaga acataataat cgtcgtctcc tatggaaaga agcaacaata
     2761 atccgttatt cttttctgcg gagcctatct tgtactcatt gaaaagttca tatgcgtaat
     2821 cggcaatgga tgccgtatgg gtataatcta ccgttacaac aacaatctgg gctcccgtct
     2881 tttcgtagag tgcaatattt ttgtcgatta ttgttctctc ggtcttctcg gataacacat
     2941 tggcggtatc attgacatag aactcggaag taggcgcagg aatatctgcg gcatataccg
     3001 cactacagac aagaaccgat attaatgtaa taaatattgc aatttttttc atgtcaatca
     3061 aacgggaaaa cattataaag gttcatcgga aatattccgc cttgtcaatg ccggttattg
     3121 atgcgataac attagccgga aacagggcca attgactgtt gaacgtatca acattcttat
     3181 taaacgatgt tctctttaag atatcgtcca cggctattat atcggcgtaa ttgcttcgcc
     3241 ttaatctctc atcggtttca ttgagactca tagtgcctaa tatgtcataa agagtcgccg
     3301 cggcattggt tattttcacc gatgccgcat atttctccgc aggtgtcttt gcattcaaaa
     3361 gcatatctct ggcttgtgcc atatttgtta tatattcgct gttgcggtca aggtatcttg
     3421 acgccacacc ggccagattg tcagcatagg ccgacttggt attcagatcg gcatccattg
     3481 tgtcgtaaaa agagttcatg accgctcttc tttgcaaaga aagagaaaca tgtccgctta
     3541 taagcaccga agcgattact gcaattacaa ccaatatcca ggctaatgct ctgttttcct
     3601 tgaatctcat cagtttttaa actccagcaa tttgttcttg agttgctcct caatcctgtc
     3661 aagttccttt tccgcttccc ttctcttagc ttttccttct tcctgaatac ggattatctc
     3721 ctcaagagtg gttatcaggc tttcgttggt ggctttcagc gtctcaatat ccacaatacc
     3781 tctttccgat tccttggcaa tgtccacact gctctgtttc agcatttccg catttttgag
     3841 aagaagttcg ttagtcatgt cggtaacggc acgctgagct ttcatggcat tctgggaatg
     3901 tgtgatgctt aatgaaagta ccatacggtt tttccaaaga ggaatggtgt ttatcagggt
     3961 ggtttgtatc ttttccgtca gaagagtgtt attgttttgt atcagtctta tctgcggagc
     4021 catctgaacg gcaatatttc tggttatctc caggtcatat atcttctttt ccactctgtc
     4081 taacatattg gcaaagtcat ttgccgcctg agcgtcttcg gggagattgg tttcttttgc
     4141 tttttttctc agctccacaa gttttgtctc tttgagttcc ttgtgtttgg ctctgccgcc
     4201 ttctatatac aaagcaagtt ctttaacata atcaaggttc atctcataca tcttgtccag
     4261 cattgtaata tctttcatca gtactacctg atgatcttcc attacagcca caatcttgtt
     4321 tacattggct tcaacctttg cataacgggc tttcaggaca tcaatatacc tttttgttct
     4381 cttgaaaagt ctctccagga aatttcccga ctcctggtcg gcggtaaaat ccttgagctg
     4441 cgccatgaga ttacctatca tgtcccctat ctctcccagt tccttggagc ggatcttttc
     4501 caatgtggca tccgagaagg ttgttatctt tctttgtgca tcggctccgt attgaagaat
     4561 ggaggcgctg cttttaatgt ccagcttgtc tccgtatgaa atagcaagtt ctctgtcttt
     4621 ctccgttaat gtactttcgt tcattgcatt tatcctttca gttattttaa attttccgct
     4681 ttcagcatac tgtcaaagac agttatttca gcatctatgt ccatagcctc atcggcatac
     4741 agattatcaa ggtacttacg gaaggaatgt ataattccgt ccattgctcc ctcaatatcg
     4801 gtttttacct gtgttatgtt ttctccgtca tcgttctgtt cttccgctct tgcgtaagca
     4861 ttcagaagtt taaccgttgt aggcagaaaa tatgtgataa gtctgcgtat atccttgacc
     4921 ttttccggtt tctcggtgat atatgcaaat atctttttta taacttcact gagtatgtca
     4981 atcttttccg acacaactgc gttttgtata cggatattgg cttttctcat tttttcaatg
     5041 tatctgtttc cttcagctat agccatatcc gcatcttttg caccggtgta ttttataggc
     5101 tcttccacca tctcaatcct gtcggggaaa aatcttccgc ttaagaaaaa ggctaatata
     5161 aaaagtattg ctgcgataat tatatgaatc aatttatgca tagggaaaat tatactgtac
     5221 agcacccata ctgcaccggc aatatatata ggtatagcgg atttatgtct tacttccata
     5281 gttttccctc tttccgtttg cagataaatt ataccataga aaaagcaaaa tactatgaac
     5341 atacaaagag agtgaaaaaa atttacaaat gaattccgtc aaattcgttg acagagaaat
     5401 acacgatgat acaatatata gcacaccatt ggtgttggta acacaaaata tagttttaca
     5461 gaggaataat catgcttacg gagaatgcaa aaaaagtact ggagaaaagg taccttaaaa
     5521 gagactcgga gggtaatgtc gtcgaatctt atgatgacat gttcaggagg gtagcaaaga
     5581 cggtagcatc cgcagatgcc gagtatttag gtaaggaaga tatagagaaa agcgaaaaag
     5641 atttctacga tatgatgata aagctggagt ttttgcccaa ttcacccaca ttaatgaatg
     5701 cgggaagacc gctggggcaa ttgtcggcct gttttgtgct acccattgaa gactccatgg
     5761 acgggatatt cgagactgta aagaatgcgg ctttaataca caaaagcggc ggaggaaccg
     5821 gtttcagttt ttccagaat
//
LOCUS       DAOPWC010000038         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_13735_length_5807_cov_3.006606, whole genome shotgun sequence.
ACCESSION   DAOPWC010000038 DAOPWC010000000
VERSION     DAOPWC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13735_length_5807_cov_3.006606"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKJ65_01540"
     CDS             complement(<1..476)
                     /locus_tag="PKJ65_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ40553.1"
                     /translation="MSIKNRIFLSSPTMHGDENRYIQEAFETNWIAPLGPNVDGFEQE
                     LAEYVGIKHAAALVSGTSALHLAVKLAGIKCGDIVFCSDLTFAATVNPVSYEGGIQVF
                     IDSEAETWNMDPRALKKAFEKYPACKCVIVVNLYGVPAKLDEIRDICDYNGAVLIE"
     gene            978..3008
                     /locus_tag="PKJ65_01545"
     CDS             978..3008
                     /locus_tag="PKJ65_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40554.1"
                     /translation="MFVRKRPWSSRYGWIWYNSKEILRDTQEDIDALVKDFADKGINI
                     LIGFSCTHFRWNFYRHWDKINECIGRLVKACHKYGIIYVEHHSCHLTFNPLNEDDLVY
                     MKSVLGKRDSYISDWPGLLEDATGDFSVDGKMISTFRQISGKTGKWGISTYHGYGMCF
                     NNPDYVAAYLKYLESLYAVGIDGIMTDDVQYFGNDGMGWREFNACTCVHCREKFKKQY
                     GYDLPSPSEWKDFYWNFNNPAFIAWKRFKDESTLEFVYKVKKHYEDLGLNQFRPNYIS
                     SILTTNKTAYPFEKCADVWDCVFQENCASSVIKESYLSFALESLHRFNLGRLNKIPSM
                     SMFYPVTKNALYFSWALAKSWGQLYTGCGGEGEGGTLDEKWLRDFEIKNMFCYTNPAK
                     ISDLAFMLSFDTRDYSNGCETHQKNFVRWLQASYLSGIATDMIFENAKFKEFAGYKRI
                     VCAHTVMLCDSVLKKLRRYVREGGHLITIGEFGKYRPDGSLRNRVGFGRKSGILGKGK
                     VTVLPDDACCKEYHWVFNIYRMETDHKVATAQAPPYAVDALRATGGKALLDAISYNTV
                     VTVTSKQDVYASLFKVRRGYALNIVNVQDTVTKSGLISHVAPIPSFVKGAKPIKHDIF
                     ININMDIPVKKITLHSPERSRCVNIPFVNENGVLTFTVPKNKFAGYALVKIK"
     gene            complement(3033..4931)
                     /locus_tag="PKJ65_01550"
     CDS             complement(3033..4931)
                     /locus_tag="PKJ65_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNZ40555.1"
                     /translation="MKRLTVILIIILVIGLTACNNEQKPSSEGYENNLLMVYFCGSDL
                     ESKNGRASANIEELLNAYIPDNTKIIIQTGGARKWKNDDINGNFIERFEIADNGLCNV
                     YHQPLNNMGEEETLTEFLNYCAKNYPDTNKSLILWNHGGGALKGCCFDENYNFDSLLI
                     TEIAKALKDADIPKLDFVGFDACLMGTVEVAYILKDCAKYMVASQELEPGCGWDYKAV
                     AESYGKNTLKETLIGIVDSYYEKCEKLGKALNATLSVIDLSEIENLANTFSKLFNTIQ
                     DNIDSVGLYSVTRPVSKASSFGGRTAYESNSNLIDLYGFADNLKLFPDKTDLLKTAIN
                     DCVIYKKNGALKQTCGGLSFYYPCNLNSSELKEMATLVVSEEYKAFLKDNYVDVGKEF
                     IRFENYGSINESGAFEITLSKQSLKFVCAVKYSLLEFAGTEFEPIIASLGTDVDIYNE
                     TPKVYTSNFKGRWVTLNGETLQCSYLGEENGYDIYSSPVKVNGEPKYLRFAFSYATRN
                     FKLLGVWAGISDSGMADKSFDDLKKGDIVTPVYYTFDANYECSTIDGNRIIISENIDF
                     QTSPLKEKYYQYVFVVEDIFGHLYYSRSAVLEMKYTYDELLANPLTGKYDFAADIIAI
                     SDNVALVP"
     gene            complement(4931..>5807)
                     /locus_tag="PKJ65_01555"
     CDS             complement(4931..>5807)
                     /locus_tag="PKJ65_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40556.1"
                     /translation="AAQEAFIASFEKNYNIPFLSDIKTFGDNLTSKAVGVGTGYCKAF
                     WGLVQGSYLWDVQTIELKEQFLNTSATAYCLAMSAAIRYLNLKNDTSMLSTLVGSFDF
                     NSKIIENYMKNLDDSMSRKRNNKTLEYSTGKTVSLTLNTYKADKDNNKTTDLSGECNI
                     KFPYENYLAKADFDKIIDAARTKKIDFLRSLRDAGFYGAPKSTEKEVRFGMEYSQSYQ
                     EFSTPISARGFSRPAQFYSGSTSSYHISYITCTGNSVSDTNTTTYAKLSRYRERNIKS
                     LIYYDTDNTYYYFMQ"
BASE COUNT         1775 a         1102 c         1065 g         1865 t
ORIGIN      
        1 tcttcaatca agactgcacc attataatcg caaatatcac gtatttcatc aagttttgcc
       61 ggaacaccat atagattcac aacaataaca catttgcatg cagggtactt ttcaaatgct
      121 tttttcaagg ctcttggatc catattccaa gtctctgctt cagagtcaat aaaaacctga
      181 attccacctt cgtacgaaac cggattgaca gttgctgcaa atgtcaaatc tgaacaaaaa
      241 acaatgtcac cacattttat tccagctaat ttcacagcca aatgtaaagc agatgtgcca
      301 gatactaaag ctgctgcatg cttaattcca acatattccg ccaactcctg ctcgaaacca
      361 tcaacatttg gacctaatgg agctatccaa ttcgtttcaa atgcttcctg tatatatcta
      421 ttttcatctc catgcatcgt tggtgacgac aaaaaaattc tattttttat actcatggaa
      481 cgcctcttta atttagtacc tataaaagcc tgcattgata acatagataa aaatttaatg
      541 tttactatgt catcatcgca agtgaaattc tactttacac cacatcaaac acttgtttaa
      601 ctgttttttt atgaaataaa ttttgcaccc cactctatcg atggcgtagc aacgatagtt
      661 tgttgtgata tatttcaccc atattcaact acgtgctccg ggcttcgtta gttgtttaca
      721 gggtcacggg taaaactaaa tattcaaaag ttattatatc attatattaa tatatataac
      781 aagtacatta tctattaatt atattcatat tgtcatgttt acctttcatt ccttgcattt
      841 tttgtcatct ttagtggtca ttatgcttta ataaaactta gtactattta atttatatta
      901 ttttcactgc ctcattgtgt atgcttttta tgcatgttat acttattctg tcaaaatatt
      961 ttacgggggt taagaatatg tttgttagga aaagaccatg gagcagcaga tacggttgga
     1021 tatggtataa ctctaaggag atattaagag atacacagga agatatagat gctttggtaa
     1081 aagattttgc cgataaaggt ataaatatac ttatagggtt ttcctgtaca cattttcggt
     1141 ggaactttta tcgtcattgg gacaagataa atgaatgtat cggtaggctt gtaaaagcat
     1201 gccataaata cggcattatt tatgtagagc atcacagctg tcatcttaca tttaatcctc
     1261 ttaatgaaga tgatttagtg tacatgaaga gtgtattggg aaaaagagat tcatatattt
     1321 ccgactggcc gggattattg gaagatgcaa ctggagactt ttccgtagac ggtaaaatga
     1381 tatcaacatt tcgccagatt tccggtaaaa ccggtaaatg gggaatatca acatatcacg
     1441 gctacggaat gtgttttaat aatcctgatt atgttgcagc atatctaaag tacttagaat
     1501 cactgtatgc cgtaggaata gacggcataa tgaccgatga tgtgcagtac ttcggaaatg
     1561 acggtatggg ttggagagaa ttcaatgcct gcacatgtgt tcactgcaga gagaaattta
     1621 agaaacaata tggatatgat ttgccctctc cttccgaatg gaaagacttc tactggaact
     1681 tcaataatcc cgcattcata gcatggaaga gatttaaaga tgaatccaca ctggagtttg
     1741 tgtataaagt aaagaaacat tatgaagatt tgggattgaa tcagttccgt cccaactata
     1801 tatcttccat acttactaca aacaagacgg catatccgtt tgaaaaatgt gccgatgtat
     1861 gggattgcgt attccaggaa aactgtgcaa gttccgttat taaagaatcc tatttaagtt
     1921 ttgcactgga atccttacat agattcaatc ttggaaggct gaataaaata ccttccatgt
     1981 ccatgttcta tcccgtaaca aaaaatgctc tatacttctc ctgggcattg gcaaaatcct
     2041 ggggacaact gtataccggt tgcggaggtg aaggagaagg cggaacactt gatgagaaat
     2101 ggcttcgtga ttttgaaata aagaatatgt tctgttatac caatcctgcg aaaatatccg
     2161 atttagcatt catgttgtcc tttgatacaa gagattattc aaacggatgc gaaactcatc
     2221 agaagaattt cgtacgatgg ttgcaagctt cttacctttc gggtatagct acagatatga
     2281 tttttgagaa tgccaagttt aaagaatttg cgggctataa aagaattgta tgtgcacata
     2341 cagtaatgct ttgtgattca gtattaaaga aattgagaag atatgtcaga gaaggcggac
     2401 acctcataac cataggagaa ttcggaaagt acagacctga cggatcttta agaaacagag
     2461 tcggtttcgg aagaaagtca ggtattttag gtaaaggtaa agtaaccgta cttcccgatg
     2521 acgcatgctg taaagaatat cactgggtat ttaatattta cagaatggaa accgatcata
     2581 aggtagcaac cgcccaagca cctccgtatg ccgtagatgc attaagagct acgggcggta
     2641 aagcactgct ggatgcaata tcttataata ctgtagttac cgtaacaagc aaacaggatg
     2701 tgtatgcaag tctgtttaag gtaagaagag ggtatgcttt aaatattgta aatgtacagg
     2761 ataccgttac aaaatcggga cttataagtc atgtggctcc cataccctca tttgtgaagg
     2821 gtgccaaacc tataaagcac gatatcttta taaatattaa tatggacatt cccgtcaaga
     2881 aaattacact gcattctcct gaacgaagca gatgtgtaaa tatacccttt gttaatgaga
     2941 acggggttct tacctttaca gtacccaaga ataagtttgc gggatatgct cttgttaaga
     3001 taaagtagcg ttaagagttt tagatatttt tattaaggca caagtgcaac attatctgaa
     3061 atcgctatga tatctgctgc aaaatcatat ttacctgtta acggatttgc taacagttca
     3121 tcatatgtat acttcatttc cagcaccgca ctacgggaat aatagaggtg gccgaatata
     3181 tcctctacta caaagacata ttggtaatat ttttctttaa gcggtgaggt ttggaaatct
     3241 atattttccg aaattataat tctgttgccg tctattgttg aacattcata gtttgcatca
     3301 aaagtgtaat atacgggagt tactatgtct ccctttttaa gatcgtcaaa ggatttgtcc
     3361 gccatacccg aatcacttat acctgcccat actcctaata atttaaagtt tcttgttgcg
     3421 taggaaaatg caaaacgaag atatttaggc tcaccgttta ctttaacagg cgatgaataa
     3481 atatcataac cgttttcttc gcctaaatag gagcattgca aagtttcacc gttaagtgtt
     3541 acccatcttc ccttaaagtt acttgtataa actttcggtg tttcgttgta tatatccaca
     3601 tcggtgccta aagatgcaat tatcggttca aattcggttc cggcaaattc caacagtgaa
     3661 tacttgaccg cacatacaaa tttcagagat tgtttgctta gcgttatttc aaaggcacct
     3721 gattcattaa ttgaaccgta attttcaaat cttataaact ccttaccgac atcaacataa
     3781 ttatctttca aaaaggcttt atactcctcc gacacaacaa gtgttgccat ctcttttaat
     3841 tcgctactgt taaggttgca gggataatag aaagaaagcc ctccgcaagt ttgttttagc
     3901 gccccgttct tcttatagat aacgcagtcg ttaatagcgg ttttgagtaa atccgttttg
     3961 tcgggaaaaa gctttagatt gtccgcaaaa ccgtataggt cgataaggtt actgttggat
     4021 tcatatgcag tcctgccccc gaatgaagaa gccttagaca caggacgggt aacggaatac
     4081 agaccgacac tgtcaatgtt atcttgtata gtattaaaca gtttgctgaa tgtatttgca
     4141 agattttcaa tttcagataa atcaattacc gaaagagtgg cattaagcgc ttttccgagt
     4201 ttttcacatt tttcataata cgagtctaca atgccgatta gagtttcttt tagagtgttt
     4261 ttcccgtaac tttccgcaac agctttgtaa tcccagccgc aacccggctc caactcttgg
     4321 cttgctacca tatattttgc gcaatctttt aggatatatg cgacctcaac ggttcccata
     4381 aggcaggcgt caaaacctac aaagtccaac ttgggtatat cggcatcctt aagtgcttta
     4441 gcaatttccg ttatcaaaag gctgtcaaaa ttatagtttt catcgaaaca gcaacctttc
     4501 agtgccccgc cgccatggtt ccagagaatt aaggatttat tggtatcagg ataatttttt
     4561 gcacaatagt taagaaattc cgttaaagtc tcctcttctc ccatattatt cagcggctga
     4621 tgatatacat tgcataatcc gttatccgct atttcaaaac gctcaataaa gttgccgttt
     4681 atgtcgtcat ttttccactt tctcgctccc cctgtctgaa ttattatctt agtattatcg
     4741 gggatatagg cgtttaaaag ctcctcaata tttgcacttg ctctgccgtt tttgctttcc
     4801 aaatcactgc cgcaaaaata taccatcaga aggttattct cataaccttc cgatgacggt
     4861 ttttgttcgt tattacaggc cgtaagaccg ataacgagaa ttatgattaa gataacggtt
     4921 aagcgtttca tcactgcata aaatagtaat atgtgttgtc ggtatcgtaa taaataagtg
     4981 atttaatgtt tctctcccta tatctggaca gctttgcata agtggtcgta ttcgtatcac
     5041 ttacactatt acctgtacag gtaatataac taatatggta actgcttgtt gatcccgaat
     5101 aaaactgtgc aggtctgcta aatcccctcg cagagatagg agtactgaat tcctggtacg
     5161 attgagagta ttccataccg aatcttacct ctttttccgt gctcttaggt gccccgtaaa
     5221 aacctgcatc tcttaaagac cgcaagaaat caattttttt agttcgagct gcatctataa
     5281 tcttatcaaa atcagctttg gcaagatagt tctcataagg aaactttata ttacattcgc
     5341 cgcttaagtc agtagtttta ttgttgtctt tatccgcttt ataggtattg agagttaaac
     5401 ttacggtttt gcctgttgag tattccagcg tcttgttgtt tctttttctt gacatggaat
     5461 catccaggtt tttcatgtag ttttctatta tcttggagtt aaaatcaaaa ctacctacaa
     5521 gagtggaaag cattgaagta tcatttttca aattcagata acgaatagcg gcagacatcg
     5581 ctaagcaata tgcggtggcg gaagtattga gaaactgctc tttaagttca atagtctgaa
     5641 catcccagag ataagaacct tgtaccaacc cccaaaatgc tttacagtac cctgttccga
     5701 caccgacggc ttttgaagta agattatctc cgaatgtttt aatatcggaa agaaagggta
     5761 tattatagtt tttttcaaaa ctggctataa aagcctcttg tgccgct
//
LOCUS       DAOPWC010000039         5775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_13843_length_5775_cov_3.421853, whole genome shotgun sequence.
ACCESSION   DAOPWC010000039 DAOPWC010000000
VERSION     DAOPWC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_5775_cov_3.421853"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKJ65_01560"
     CDS             complement(<1..1100)
                     /locus_tag="PKJ65_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40557.1"
                     /translation="MKKLSKKKIIAVMTVILITAMLMTSCVKLPFPGGKGKTSPEPSQ
                     TITETASPVQDPTATPKPDQSQEPTATPVETQTQAPTPTPTPTATPAPTATATPTPTA
                     TPTPTPTATPAPTATPTPTPTPTPTPTATPTPTPTATPTPTPSAAPFGIGNAYASGPK
                     EITVEFSAPAQAGAQVVLKTGGINVTGTSLNWASDRRSVRITRTSAYAAGEYTVTVSD
                     SSKTFTVQAEKLTAISITAQYVFPMDNQDLKVVFTNQYGGEMPLTAYTNFTLSAVCQA
                     KPSVTLNLNKTAAGFILKGAQGLAANDSVNIVIAENTTGTAVTKSITLSESPKITAFA
                     FGQVQLASGATVILVNSSGHSLTVDAKDQYNQA"
     gene            complement(1125..2258)
                     /locus_tag="PKJ65_01565"
     CDS             complement(1125..2258)
                     /locus_tag="PKJ65_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40558.1"
                     /translation="MNSRERLITVLKGEIPDHVPVSPDISQMIPIRRTGLPYWDIYLY
                     QRIPKWKAYIDSVKYFGFDSLMDGFANIQFEELGEFYNPWTEVIVHKDQLKIITQKYR
                     TEKNEKVWEPLVSVYHRDSPPSWDVHPHLVKLPLIPDRYEPVEDVSQWPTGEALLKWV
                     KEEMGNHGLVGVTCGTSIVLKNSEMVYDFYDNPDKYYKKRDELIEYYEKRLHKLMSLE
                     HKPDFIGTGGSGSLIFQTPEIFRELALPIVKRVAKLCKQYNIPSHVHSCGPQKEMVKM
                     LAEETELTVIDPLEIPPMGDCNLKELKKLYGDKIVLKGNLHTTEVMLRGSVQDVTDAC
                     KRAIDDAAEGGKFILSTGDQCGRDTPDENLFAMIETAKTYGRY"
     gene            2385..2627
                     /locus_tag="PKJ65_01570"
     CDS             2385..2627
                     /locus_tag="PKJ65_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNZ40559.1"
                     /translation="MNINTDTIVSMTEANQNFSKVARLVDEKGMAVIMKNNAPRYLVM
                     EFKQLKEQQLASDEDVLTLSKRFIDSNRKAYTELAK"
     gene            2752..4020
                     /locus_tag="PKJ65_01575"
     CDS             2752..4020
                     /locus_tag="PKJ65_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40560.1"
                     /translation="MKKEPGKTVNLAFVGLGKRGLSLLNLVLVAMDDIKVTALCDVYQ
                     DRTDRAADLVEEKTKERPFTTLDYKELLDRKDIDAVIVPSSWETHFDICLDFMKAGKY
                     VASEVGGANSVEQCWDLVKTYEKTGTPAMILENCCYGKTELTVLNMIKQGVFGELIHA
                     QGGYQHDLRTEVATGVEKRHYRLDNYKHRNGDLYPTHELGPIAKWLSVNRGNRMTYLC
                     SMASKTAGITDWIRKNRGPDYDNANFKFALGDVVTTMIKCAHGETILLVHDTSLPRPY
                     SRGNRLQGTNAIWMEDKWAFCIDGVSPVNKWGEYRWESFDDYYEKYEHPLWTWYRQRG
                     LIGGHDGMDYLTLRAFLEAVKNGTDTPIDAYDMATWMSITCLSEQSVAMGGHPVPIPD
                     FTNGRWMHREPAIPGRYCLDEICGPEYFEI"
     gene            complement(4034..5170)
                     /locus_tag="PKJ65_01580"
     CDS             complement(4034..5170)
                     /locus_tag="PKJ65_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HNZ40561.1"
                     /translation="MKRKKKKRSKTKNTNTVKAVTMLVAALCFAFLTAVLVTILKRVP
                     GFTNGIYTKWSVFAMHTLANFFSFVKFSVAEVLIYILSVIFLSSLVILIVNLIKKKYR
                     VTRIFKFITAWMLVVSVTVFLFYALWGGMYYSKGIGNYLPVNSEDIKERPIEELINLN
                     KYLIEKTNRLAAEVPRNEQGYYTGYEFQRTAAFTAKEVSKITGKEEAPPKYVVSSDAW
                     SYTQTTGIFIFLTGEANINKNNLAIALPFTCAHELAHRNGITSEDEANFFAFYSLHDS
                     ANINLRYSAYAMTLMYTMNSLYSKDAAAHKELASTYCDLLKHDYIRYHEHWDRYEGKT
                     SEISDKVNDTYLKAQGQSDGIYSYGRVTDIMLAWYENDIVKNKK"
     gene            complement(5142..5309)
                     /locus_tag="PKJ65_01585"
     CDS             complement(5142..5309)
                     /locus_tag="PKJ65_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNZ40562.1"
                     /translation="MNPLDYILIAIVALIVLSIVFYLIKSRKKGGSHNKCSACPYSGQ
                     CSDEKKKEEEK"
     gene            complement(5314..>5775)
                     /locus_tag="PKJ65_01590"
     CDS             complement(5314..>5775)
                     /locus_tag="PKJ65_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNZ40563.1"
                     /translation="TKAFTIIFVASIVIWFLNKFDIRFNVVSDSKDSMLAAIGSLIAP
                     VFTPLGFGNWKASTALLTGFMAKEAVVSTLAVLLSTGMEKLSAPLATLFTPVSAFSFL
                     LFTLLYTPCIAAVAAVRRELGTRYMFIAIAFQTGLAYIVAMVFYQIAMLAG"
BASE COUNT         1584 a         1190 c         1309 g         1692 t
ORIGIN      
        1 gcctgattat actggtcttt tgcatctacc gtaagcgaat gccccgaaga gttgacaaga
       61 ataacggtag ctcccgatgc cagttgtacc tgtccgaaag caaaggcagt tattttaggc
      121 gattcgctta atgttatgga tttggttacc gcagttccgg tagtattctc cgctattaca
      181 atattgaccg aatcattggc tgcaagtcct tgagctcctt taagaatgaa gccggcagct
      241 gttttgttta aattgagagt taccgaaggt tttgcctgac ataccgcact taatgtgaag
      301 tttgtgtatg ccgtcaaagg catttcccct ccgtattggt tggtaaacac aactttcaaa
      361 tcctggttat ccatagggaa aacatattga gcggttatac tgatagcggt aagtttttcc
      421 gcctgcaccg taaaggtttt acttgaatcg gatacggtaa cggtatattc gccggctgca
      481 tatgcggaag tccttgttat ccttaccgaa cgtctgtccg atgcccagtt taaactggtg
      541 ccggtaacat tgattcctcc ggttttcaac acaacctgag cgcctgcctg agcgggtgcg
      601 ctgaattcca cggttatttc cttaggaccc gatgcatagg cattacctat accgaacgga
      661 gccgcagagg gtgtcggagt aggagttgcc gtaggagtcg gagtaggtgt agccgtagga
      721 gtaggagtag gcgtaggagt cggagtaggt gtagccgtag gagcaggcgt tgccgtagga
      781 gtaggagtcg gtgtagccgt aggtgtaggc gttgcagtag ccgtaggagc aggtgtggcc
      841 gtaggcgtag gtgttggtgt cggtgcctgt gtctgcgttt ctaccggcgt tgcagtgggt
      901 tcttggctct gatcgggttt aggtgtagcc gtagggtcct gtaccggact tgccgtctcc
      961 gtaattgtct gtgacggttc gggtgaagtc tttccctttc cgcccgggaa aggaagcttc
     1021 acgcaggaag tcataagcat agcggttatc aatattaccg tcattaccgc aattattttt
     1081 ttcttactta gttttttcat tttttacccc cgttatgtta tttttcaata tctgccgtag
     1141 gtcttggcag tttctatcat tgcaaacagg ttttcatcag gcgtatctct gccgcactga
     1201 tcacctgtag atagtataaa tttaccacct tccgccgcat cgtcaatagc tcttttgcat
     1261 gcgtctgtta catcctgaac cgaacctctg agcattactt ccgtggtatg aaggttgccc
     1321 ttaagcacta ttttgtcccc gtagagtttt ttcaattctt tcagattaca gtcacccatg
     1381 ggcggtatct ccagaggatc tattacggtc agttcggttt cttccgccag catttttacc
     1441 atctctttct gcggaccgca ggagtgaaca tgcgacggta tattgtactg cttacacagt
     1501 tttgccactc ttttaactat gggcaaagcc agttccctga aaatctccgg tgtctggaaa
     1561 atcaacgatc cggaacctcc cgtgcctata aaatccggtt tgtgctccaa agacattaat
     1621 ttatgcagac gcttctcata atattctatc agttcatcac gctttttgta atacttatcg
     1681 ggattatcat aaaaatcata caccatttcg gagtttttta acactatgga tgttccgcag
     1741 gtaactccca caagtccgtg attgcccatt tcttctttaa cccactttaa cagagcttct
     1801 ccggtaggcc attgcgatac atcctctaca ggttcatacc tgtcgggaat aaggggaagt
     1861 ttgacaagat gaggatgaac atcccaggaa ggagggctgt ctctgtggta tacgcttacc
     1921 agaggttccc ataccttctc gtttttctcg gtacggtact tctgcgttat tatcttcagt
     1981 tgatctttgt gcactatgac ttcggtccag gggttgtaaa attcgcccag ttcctcaaac
     2041 tgtatattgg caaatccgtc catgagagaa tcaaaaccga aatatttgac actgtcgata
     2101 tatgctttcc atttggggat tctctgatat aggtatatat cccagtaagg aagaccggtt
     2161 cttcgtatag gaatcatctg agagatatcc ggagataccg gcacatggtc gggtatttct
     2221 ccttttaata cggttataag tcgctctctc gaattcatag ttgcctcata catacgccgt
     2281 tattatatac taattttaac ataagataaa taagataaat aattgcaatt ataggttgta
     2341 tttatcctat atgcaaacta taattaagac aggagggact cataatgaat attaataccg
     2401 acacaattgt ttctatgacc gaagccaatc agaacttttc taaagtggca agactcgtag
     2461 atgaaaaagg catggctgtt attatgaaga ataatgctcc tcgctatctg gttatggagt
     2521 ttaagcaact taaagagcaa cagcttgctt cggatgagga tgttctgact ttatcaaaac
     2581 gcttcatcga cagcaatcgt aaagcatata cggagcttgc aaaatgattg gtctgatatt
     2641 gtattgtata tgctgttcaa agtgatttaa tccatgtaaa attatgctat aataccaaag
     2701 agtgtattga tatagtacgc gaaagaacat atataatttt cggaggtaga tatgaagaag
     2761 gaaccgggta aaacagtgaa tcttgctttt gtgggattgg gtaaaagggg attgtcgtta
     2821 ttgaaccttg ttttggttgc aatggatgat ataaaggtaa cggcattatg cgatgtttat
     2881 caggacagaa ccgacagagc agctgatttg gttgaggaaa agacaaaaga aaggcctttt
     2941 acgacacttg attacaagga actcctggac cgcaaagata tagatgcggt aattgtaccc
     3001 tcctcatggg aaacacattt tgatatatgc ttggatttca tgaaagccgg caagtatgtc
     3061 gcttcggaag tgggaggagc caattccgtt gaacaatgct gggatctggt taaaacatat
     3121 gagaaaaccg gtacacctgc catgatattg gagaactgct gttacgggaa aaccgaactt
     3181 acggtattga atatgataaa acagggtgtt ttcggagaac tgattcatgc gcagggaggt
     3241 tatcagcatg atttacgaac cgaagtggct accggtgtgg aaaagcgtca ttacagactg
     3301 gataattaca agcaccgtaa cggcgatctt tatcccacac atgagttggg accgatagca
     3361 aaatggcttt ccgtaaacag aggcaacaga atgacttatc tttgttccat ggcttcaaaa
     3421 acagcgggaa taactgattg gatcagaaaa aacagaggac cggattatga caatgcgaat
     3481 tttaaatttg ccctcggcga tgtcgtgacc accatgataa agtgtgcaca cggcgaaacc
     3541 attctgcttg tgcacgatac ttctttaccc agaccgtatt ccagaggtaa caggcttcag
     3601 ggtacaaacg caatatggat ggaagacaag tgggcattct gtatagacgg agtcagtccc
     3661 gttaataaat ggggcgaata ccgttgggaa agtttcgacg actattatga aaagtatgag
     3721 catcctctgt ggacatggta caggcaaaga ggtctcatag gcggacatga cggaatggat
     3781 tatcttactt tgagagcttt tctggaagct gttaagaacg gtaccgacac tcccatcgat
     3841 gcatatgata tggctacctg gatgagtata acctgcttat cggagcaatc ggtggctatg
     3901 ggcggtcatc cggtacccat tcccgatttc acaaacggca gatggatgca cagagagcct
     3961 gcaataccgg gcagatattg tctggatgag atctgcggtc ccgaatattt tgagatttag
     4021 ttataacagt taatcatttt ttgtttttaa ctatatcatt ttcataccat gccagcatta
     4081 tatcggttac gcgtccgtaa ctgtaaatgc cgtctgactg accctgagct ttcagatagg
     4141 tatcattaac cttgtcggat atttccgatg tctttccttc atatctgtcc cagtgctcgt
     4201 gatatcttat atagtcatgt tttaataaat cgcagtatgt cgatgcaagc tccttatgtg
     4261 ccgcagcgtc tttggaataa agagaattca tggtgtacat caatgtcatg gcataagcgc
     4321 tgtaacgaag attaatatta gccgaatcgt gcagagagta gaatgcgaag aaattggctt
     4381 catcttccga tgtgatgccg tttctgtgcg caagttcatg tgcacaggta aagggcagtg
     4441 caatagccag attattttta tttatgtttg cctctccggt aagaaaaatg aatatgccgg
     4501 ttgtctgggt atatgaccat gcatccgaag ataccacata cttaggagga gcctcttctt
     4561 taccggttat tttacttacc tctttggccg taaaagcggc ggttctttgg aattcatacc
     4621 ccgtgtaata tccctgctcg tttctgggca cctcagccgc cagccggttg gttttttcaa
     4681 tgagatattt attgaggttt ataagttctt ctataggtct ttcctttata tcttcactat
     4741 tcaccggtaa gtagttgccg atgcccttgg aatagtacat gcctccccat aaggcataaa
     4801 agagaaaaac cgttaccgac accaccagca tccaggcggt aataaacttg aatatgcggg
     4861 ttactctgta ttttttcttt ataaggttga caatcagtat taccaaagaa gacagaaata
     4921 ttacagagag gatatatatg agtacttccg ctacggaaaa cttaacgaaa gaaaaaaagt
     4981 ttgccagagt atgcatcgca aaaacagacc acttggtata gataccgttt gtgaaaccgg
     5041 gaactctctt taatattgta accagaaccg ctgtcagaaa tgcaaagcac aacgccgcta
     5101 caagcatggt gacggctttt acggtgttgg tgtttttagt tttacttctc ttcttctttt
     5161 ttcttttcat cactgcattg tccgctgtac gggcatgccg agcacttatt gtgtgagccg
     5221 cccttttttc tgctttttat aaggtaaaag actattgata aaactattaa agcaacaatt
     5281 gctattaaaa tataatctaa aggattcata atatcaaccc gctagcattg caatctgata
     5341 aaacaccata gctacgatat atgccagacc tgtctgaaaa gctatggcta taaacatata
     5401 cctggttccc aattcccgtc gtaccgcagc aaccgcagcg atacacggcg tatatagaag
     5461 agtaaacaac agaaaggaaa atgccgaaac cggagtgaaa agggtagcca gcggagccga
     5521 taacttttcc attcccgtac ttaacaatac cgccaatgtg gatactaccg cttcttttgc
     5581 cataaaaccg gttaaaaggg cggtggaagc cttccagtta ccgaatccca aaggagtgaa
     5641 aacaggagct ataagcgagc ctattgcggc aagcatactg tctttactgt cggagacgac
     5701 attgaatcgg atatcaaact tattcaaaaa ccatatcact atggatgcga caaatattat
     5761 ggtaaatgct tttgt
//
LOCUS       DAOPWC010000040         5734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_13965_length_5734_cov_3.722134, whole genome shotgun sequence.
ACCESSION   DAOPWC010000040 DAOPWC010000000
VERSION     DAOPWC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13965_length_5734_cov_3.722134"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKJ65_01595"
     CDS             <1..506
                     /locus_tag="PKJ65_01595"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ40564.1"
                     /translation="TGVVLFAKNSVVHHKMSITDIDKRYIGMVIGIPSLDETTINMPI
                     ARVPDTPMERHFSPTGKQSVTHYEVLYKGHNASILSFRLETGRTHQIRVHCKESGFPI
                     IHDTLYGYQCEYDRYISRQALHSCSVSFIHPFTNEYITITSPLPEDMAGLEKAIKEKS
                     LVNEQNR"
     gene            521..1249
                     /gene="recO"
                     /locus_tag="PKJ65_01600"
     CDS             521..1249
                     /gene="recO"
                     /locus_tag="PKJ65_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNZ40565.1"
                     /translation="MEYLKLKGIVCKITEYKEADKILTLLTYEKGKVQVIAKGVKKKG
                     AALSHVARLFYCGEFECVCKNNMPILTGAAMIQDFFGLTNNIETLYYASHFMDVCSCF
                     IQAEQPSGDIMSLLLNSLYLLMKSNSDPLLLTCILHFRMSAIEGAAPVTDFCVECNKP
                     LDDNCTLGFSLEQDGVTCCKNGKSISGAVYQAINHICTADSSKIFFLSIPKTDLPVLY
                     ELSCKYIEKIAGRHFEILDRIEEL"
     gene            complement(1263..2162)
                     /gene="era"
                     /locus_tag="PKJ65_01605"
     CDS             complement(1263..2162)
                     /gene="era"
                     /locus_tag="PKJ65_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNZ40566.1"
                     /translation="MGFKSGFVTVIGRPNVGKSTLINALSEEKISIVTPKPQTTRKNI
                     KAIYTDDFCQMVFCDTPGIHEPKTRLGEYMVNAAKSAFEETDVIIFITALQKGEKIPK
                     EDVHILQLLSEVKNIPKIFVLNKCDTIDQQTAVSIIALYTEKAAFDEVIAISAKDGFN
                     IKPLKTLIIDNLPEGPMYYDKDQITDQTMREMAEEMIREKMLYFLSEEVPHGVAVEVE
                     NFRQRPDMQLYDIDAIIYCEKETHKGIIIGKGGAMLKKIATQARKSMEYSFDCKVNLK
                     LWIKVKSDWRNDSRMLNKLGYKF"
     gene            complement(2164..2574)
                     /gene="cdd"
                     /locus_tag="PKJ65_01610"
     CDS             complement(2164..2574)
                     /gene="cdd"
                     /locus_tag="PKJ65_01610"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNZ40567.1"
                     /translation="MKDKYEELKQKAKEAVKNAYSPYSDIKIGAAVLASSGKIYSGAN
                     IENSSYGASICAERTAIVKAISEKEQSVTAIAITGDVFDYAYPCGICRQVMSEFMQED
                     APVFVSGNNLEWKRYTLKELLPDAFDIKAWKKEE"
     gene            complement(2564..3055)
                     /gene="ybeY"
                     /locus_tag="PKJ65_01615"
     CDS             complement(2564..3055)
                     /gene="ybeY"
                     /locus_tag="PKJ65_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNZ40568.1"
                     /translation="MKTRIDIEGPDEVTALFSRPVKEVSESILARHETDDKDITVSFY
                     LEFVDDEYMLEINKAYRNIEKTTDVLSFPALNCKEGTIVYNKCDIDYENNELFLGDIL
                     IATSKIYSQAESYNHSPLRECVFLASHGLLHLLGYDHEDKQSEAVMLAIQEEVLKELN
                     YER"
     gene            complement(3039..5192)
                     /locus_tag="PKJ65_01620"
     CDS             complement(3039..5192)
                     /locus_tag="PKJ65_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ40569.1"
                     /translation="MNKTKAAKRDLFKTKLYSIVILLLAIMVALLGILQKVSDAGHLI
                     DRHNIATTDIVASHDYYDKEATIAKANKAKDSVEPIAVRINSSQVALYENIFTFFDVT
                     NRYREKTEQLFLTSQQYNAEIDGLVKQYTGMLPSSINISHNLAKAILQDVNSTSYDSY
                     KTVVRTQFSVFMDKYIFYENLAQHQEEFEVAVNQSVPDNNLKELTAVLKSYLIQINSR
                     IDTGATEKERQRVYDDVITNSPVLIMEGTVLYQKGEKLENEDILALYERGVIAASDEM
                     YMYIGATVLFLLCLLASFASVKIYGQTVRLNPKSIFIMVILFFMVYIPALYIPHEYYM
                     YIPYFLLPMMLSILVSDNISYIFSGPMAIFYCYIVGGDLIYLAILLIGSIATIYFVRK
                     TSARIRLAISGIALGVINVALYMSYKLITSDVTISIPYPEIIALFVASVVSSLLCLGL
                     LPVFEVFFNIVTPFKLMELASPTKPLLKRLMMEAPGTYHHSLMVGNLAEEAAEAIGAN
                     GLLARVGAYYHDIGKLNRPAFFGENQSSKTGNPHNTITPQLSATIITGHTTEGADLAK
                     QAKLPPSIRDIIVEHHGTTLVEYFYHKAKNASDEPVIEEDYRYSGPKPRTKESACVML
                     ADACEATIRSMENTNIIEIEKTIRRVAKTKRDDGQFDQCDLTLKELDEILFAFMKVFT
                     GYFHKRIKYPNQQSDDAVKSMGLDRKEDDRDEDED"
     gene            complement(5189..>5734)
                     /locus_tag="PKJ65_01625"
     CDS             complement(5189..>5734)
                     /locus_tag="PKJ65_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ40570.1"
                     /translation="LAVACAVTAFRAKAVKRIILTRPAVEAGEKLGFLPGDLQSKIDP
                     YLRPLYDALFELMGPDNVARNKEKGLIEIIPLAYMRGRTLDDAFIILDEAQNTTQEQM
                     KMFLTRMGFGSKMVINGDITQIDLPRDKKSGLITAVQILKNIEDIGIVNLTFKDVVRH
                     ELVQKIINAYERAESKKGERK"
BASE COUNT         1552 a         1232 c         1100 g         1850 t
ORIGIN      
        1 ctacaggggt ggtgcttttt gcaaagaatt ccgtcgtgca tcataaaatg tcaatcacag
       61 atattgataa aaggtatata ggcatggtta tagggattcc gtctttagat gaaacaacca
      121 taaatatgcc cattgcaagg gtacctgaca caccgatgga gcgacatttc agtccgacag
      181 gaaagcaatc ggtaacccat tatgaggtat tgtataaagg gcataatgca agcatattat
      241 cttttcgctt agaaaccgga agaacccacc agatacgggt gcattgtaaa gaaagcggtt
      301 ttcccataat tcatgatacc ttatacggtt atcagtgcga atacgacaga tatatatcca
      361 gacaggcttt gcactcttgc tctgtatcgt ttattcatcc tttcacaaat gaatatatta
      421 caatcacttc tcctcttccc gaagatatgg caggtttgga aaaggcaata aaggagaaat
      481 cacttgttaa tgagcaaaac aggtgatatg ctttgaaata atggagtatt taaaactaaa
      541 aggcattgtt tgcaagataa ccgaatacaa agaggcggat aaaatattaa cccttcttac
      601 ctatgaaaaa ggaaaggtgc aggttatagc caaaggagta aagaaaaaag gtgcggcact
      661 gtcacatgtg gcaaggcttt tttattgcgg cgagtttgaa tgtgtatgta agaacaatat
      721 gcctatactc accggagcgg caatgataca ggacttcttc ggtctgacaa acaacattga
      781 aacactgtat tacgcttcac actttatgga tgtctgctcc tgtttcatac aggcggaaca
      841 accttctggg gatataatgt cgcttcttct gaacagcctt tatctgttga tgaagagcaa
      901 cagcgaccct ttgcttttaa cctgtatact gcactttcgc atgtcggcaa tcgaaggtgc
      961 ggcacctgtt accgatttct gtgtagaatg caacaaaccg ttggatgaca attgcacttt
     1021 gggcttttct ttggagcagg acggtgtgac atgttgcaaa aacggaaagt ctatatcagg
     1081 tgccgtttat caggcaataa atcacatctg cacggccgat tcgtcaaaga tattcttttt
     1141 aagcatacct aaaaccgatt tgcccgttct gtatgaacta tcctgtaagt atatagagaa
     1201 aatagccgga aggcattttg aaatattgga cagaattgag gaactatagg tttacttctt
     1261 ttctaaaatt tatatcctaa tttatttaac atcctgctgt cgtttcgcca gtcactcttt
     1321 actttaatcc acagttttaa atttactttg cagtcaaagg aatactccat ggattttctg
     1381 gcttgcgttg ctatcttctt cagcatagcc ccgccttttc ctattattat ccccttatga
     1441 gtttcttttt cacagtaaat gattgcatcg atatcataca gctgcatatc cggtcgttgc
     1501 cggaagtttt ccacttccac ggccacaccg tgaggcacct cttccgacag aaaatacagc
     1561 attttttctc ttatcatttc ttccgccatt tctctcatgg tctgatcggt tatctgatcc
     1621 ttgtcataat acataggtcc ttcgggaagg ttgtctatta tgagggtttt gagaggcttg
     1681 atattaaatc cgtcttttgc acttattgct ataacttcat caaaagcggc tttttcggta
     1741 taaagagcaa taatactgac ggcagtttgt tggtcaatag tatcgcattt gttcagaaca
     1801 aatattttag gtatattctt cacttcggac agaagttgta atatgtgaac atcctcctta
     1861 ggtatctttt cgcccttttg caaagcggta ataaaaataa tcacatcggt ttcttcaaat
     1921 gccgatttgg cagcattaac catatactct cccaatcgcg tcttgggctc atgaattccg
     1981 ggagtatcgc aaaaaaccat ctgacagaaa tcgtcggtat atatggcttt gatgtttttt
     2041 cttgttgtct ggggtttggg tgtaacaatg cttatctttt cttcgctaag agcatttatg
     2101 agggtggatt tacctacatt aggtcttcct ataacggtaa cgaatcctga tttgaaaccc
     2161 atactactcc tcttttttcc acgccttaat gtcaaaagca tcgggtaaca gttcttttag
     2221 cgtgtacctc ttccattcaa ggttattgcc gcttacgaaa acaggagcat cttcctgcat
     2281 gaattcgctc attacctgac ggcatatccc gcacggatat gcatagtcaa acacatctcc
     2341 ggttatggct atagcggtga ccgattgctc tttttcgctt attgccttta caatagccgt
     2401 tcgctcggca catatggacg ctccgtaaga cgagttttca atattggcac ccgaataaat
     2461 cttcccgctt gatgccagaa ccgcagcccc tatctttata tccgaatagg gtgaataggc
     2521 atttttaaca gcttcttttg ctttttgttt taattcttca tatttatctt tcatagttta
     2581 actctttcag aacctcttcc tgaattgcaa gcattactgc ttccgactgt ttatcttcat
     2641 ggtcgtagcc cagaagatgc aacagtccgt gacttgcaag aaaaacacac tcccgtaacg
     2701 gactgtggtt ataactttcc gcctgtgaat atatcttgct tgttgctatt aaaatatctc
     2761 ctaagaacaa ttcgttattc tcgtaatcaa tgtcgcattt gttataaaca atagtacctt
     2821 ctttacagtt gagtgcggga aaagacaata catcggtagt cttttcaata tttctatatg
     2881 ctttatttat ctcaagcata tattcatcat ccacaaactc caggtaaaaa gatactgtta
     2941 tgtctttatc atctgtttca tgtcttgcca gtatgctttc cgatacttct tttaccggcc
     3001 gggaaaacag ggccgttact tcatccggtc cttctatatc aatcctcgtc ttcatcccgg
     3061 tcatcctcct tacggtccaa gcccatgctt tttacagcat catccgactg ctgattggga
     3121 tactttattc tcttatgaaa atatccggta aacactttca tgaaagcaaa cagtatttca
     3181 tccagctctt ttaatgtaag atcgcattgg tcaaactgtc cgtcatccct ctttgttttg
     3241 gccactcttc ttatggtctt ttcaatctct ataatattgg tgttttccat agatcttata
     3301 gtggcttcac aggcatctgc aagcatgaca catgccgatt ccttggttct gggtttgggt
     3361 ccggaatatc tgtaatcttc ctctataacc ggttcatccg aagcattctt agctttatga
     3421 taaaaatatt ccactaatgt cgttccgtgg tgctccacta tgatatccct tatggaaggc
     3481 ggaagtttag cctgttttgc caaatccgct ccctcggtag tatgaccggt tataattgtg
     3541 gccgacagtt gcggtgttat cgtattatga ggattacctg tcttggaaga ttgattttct
     3601 ccgaaaaatg cgggtctgtt cagcttgcct atgtcatgat aatatgctcc gactctggct
     3661 aaaagaccgt tggcccctat cgcttcggct gcttcctcgg caaggttacc caccatcagc
     3721 gaatgatgat atgtcccggg agcttccatc ataagtctct tcaacagagg ttttgtggga
     3781 gatgccagtt ccataagttt gaaaggagtg acaatgttaa agaatacttc aaataccggc
     3841 aacagtccga ggcataaaag tgaagaaacc accgatgcga caaacagagc tataatctcg
     3901 ggatagggaa tacttatcgt tacatcggaa gttataagtt tgtatgacat gtatagagcg
     3961 acatttatca cacccaatgc tattccggaa atagccagtc ttattcttgc ggaagtcttt
     4021 cttacaaaat atatggtggc tatggagcct ataagcagaa ttgccagata tataaggtct
     4081 cctcccacaa tgtagcaata gaatatcgcc ataggaccgg aaaagatata tgaaatgttg
     4141 tcggatacaa ggatggagag catcatgggc aacagaaaat acgggatata catatagtat
     4201 tcatgaggta tatacagagc gggtatatat accataaaga acaatatgac cattatgaat
     4261 atggacttgg gattaagtct cacggtctgt ccgtatattt ttaccgatgc gaaagaagcc
     4321 agaagacaca gtaaaaacag cacggtagcc cctatataca tatacatctc atcggatgcg
     4381 gcaatcactc ctctttcgta aagcgccaat atatcctcgt tctccagttt ttcacctttt
     4441 tggtataaaa ccgtcccttc catgatcaat acgggtgagt tggttataac atcgtcatat
     4501 accctttgcc gttccttttc cgtcgcaccg gtatctatcc tgctgttaat ctgtatcaga
     4561 taggatttaa gaacagcggt aagctctttt aaattattgt cgggaaccga ttgatttacc
     4621 gcaacttcaa attcttcctg atgctgtgca agattctcgt aaaatatgta tttatccata
     4681 aatacggaaa attgcgtacg gactaccgtt ttgtaactgt cataggaagt gctgttaaca
     4741 tcctgcagta tggcctttgc cagattgtgc gagatattta tgcttgacgg caacatgcct
     4801 gtatactgtt ttaccaaacc gtcaatttcc gcattgtatt gctgcgaagt gagaaacagt
     4861 tgctcagtct tttcgcgata gcggttggtt acatcaaaga aagtgaatat attttcatat
     4921 aacgccactt gtgacgaatt tatccttacg gcaatgggtt ctaccgagtc tttggctttg
     4981 ttggcttttg ctattgtggc ttccttgtcg tagtagtcat gtgaagctac tatatcggtt
     5041 gtggctatgt tatgtctgtc tatcagatgt cccgcatcgg acaccttttg caatatacct
     5101 aatagcgcaa ccattattgc caacagaaga attacaatgc tgtacagttt tgtcttaaat
     5161 aaatctcttt ttgcagcttt ggttttgttc atttgcgctc acctttttta ctctcggcac
     5221 gttcatatgc atttattatc ttttgcacca actcatgtct tactacatct ttaaaagtaa
     5281 gattgactat gccgatatct tctatgtttt taagtatttg tacggcagtg ataagtcccg
     5341 attttttgtc tctcggaagg tcaatctgag ttatgtctcc gtttataacc atcttggaac
     5401 cgaaacccat tctggttaaa aacatcttca tctgttcctg tgtggtattc tgggcttcat
     5461 ctaaaatgat gaatgcatca tccaatgtac gccctctcat atatgctaaa ggtattatct
     5521 ctattagccc tttttctttg tttcttgcaa cattgtccgg ccccatcagt tcaaacaggg
     5581 catcgtacaa cggtcgtaaa tagggatcta ttttagactg taaatcaccg ggcaaaaatc
     5641 ccagtttctc acccgcttcc accgcaggtc ttgtaagaat aattcttttt accgcttttg
     5701 ctctaaaagc cgttaccgca caggccacag ccaa
//
LOCUS       DAOPWC010000041         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14033_length_5717_cov_2.589014, whole genome shotgun sequence.
ACCESSION   DAOPWC010000041 DAOPWC010000000
VERSION     DAOPWC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14033_length_5717_cov_2.589014"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKJ65_01630"
     CDS             complement(<1..815)
                     /locus_tag="PKJ65_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HNZ40571.1"
                     /translation="MSGVINRYAVLHINSGTYAYAIDSHTLCIRLRTAKNDCKSVIVH
                     YMNVYDHSTNRKKENMAKILQDEHFDLYEAKISQKEKHFKYFFELIGEKEQVFYTSDG
                     FMGNEVKNENSFFFPVINDDEIYKLPKWAEGATVYQIFVDRFNSSCDKDFVNADWNDK
                     PLRSHFFGGDFKGIEYKLDYIKSLGIDIVYLSPIFSSATNHKYDIEDYYSIDEMFGGR
                     KELSNLIQSIHKKGMRIVLDCVFNHMSNNNKIFRDVLEKGQNSQYKDWFYIHD"
     gene            complement(812..1645)
                     /locus_tag="PKJ65_01635"
     CDS             complement(812..1645)
                     /locus_tag="PKJ65_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ40572.1"
                     /translation="MRKKNKLFRNIGVHAILIVLALIVLIPFVYILLISFGKDLLDIT
                     GYGDMGFSVDNYEKLFTETEYLHWLGNSLLVSLATMLISLVMVSVASNVFSKYKFAGR
                     EALFRTMLLIQIFPLTLMLVSLFKIFAALELLNKLIGLVIIDSVIATAGLILVSKGYF
                     DTIPYEIDEAAMIDGANRFQVLIHITLPLVKPILGIVAIQSFVLTYNEYAIASMLITS
                     GAEKMTVAVGLQSLIYGQFQQNWGMYSAGAVLASLPIFILFYSFQRYFIGGLTEGGVK
                     Q"
     gene            complement(1662..3980)
                     /locus_tag="PKJ65_01640"
     CDS             complement(1662..3980)
                     /locus_tag="PKJ65_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ40573.1"
                     /translation="MRKRTVLAILTVAIILSVCFCNIVKADGDKVITVWGSEIKREND
                     VIREIAQQFKIETGITVSVIDRRSLFDAPRDLVNNAKTGEKPDIVMIQHADIGDLAVN
                     GFLLELDFTEEFRLAYHESAFTSFEVRDKQYGIGYSVETYGLVYNRDVISKEQIANLT
                     WDEFFQYAIDFGTTTHNGNPMKAFVLNPKNMYFMYPIIKNYGGYYYGVDENGNYNPFD
                     VGLSNEGSKKYVEKIKNLLDHELVLKSRNGTDSDVSAAFANQDVAIIINGFWQNSFYR
                     QKGVNFGFAPLPLNNNKTATSPFATVNGFVINAHSDYPDEAKKFLEFMCRDEYQQMLI
                     EAGNNFEKKVGSRFPVNLSVRESNYIKSEEMYDVMADICENIEPVPNIPEGAIWYHYT
                     DKAYQSIFYGDINGNPVDSDSMLEELSKAILNDVKKMNVQVEKVEIPVLGYVFIGLFF
                     VILLAILIITFIRRRKGYSASANRIMLRATVVSYIALFPVLVLIGIFYLFPIVHNIYL
                     SMTNYSSLNLIDYVFIGGHNYKTIFIEGLNGFVSMFIWTVIFATSVMVITFLGGTMLA
                     SLLEKRKAKVSKIYKMIFILPWVIPSVITLLTWQGILATDGGALNNLLSLLGIGKVMW
                     LDNPFLARVCTIFVVSWMGTPYYMFLALGYLQSIPKDFYEAAMIEGAGRVKVFFIITV
                     PIIFRSLLPTLIMGFIMQFNNFGVYLLTSGGPFGSTLGAPGATDLLITYVYNLAYNTK
                     NYGLAAAYSVIIFLFVAVFTLITMKLGNKKNP"
     gene            complement(3994..5049)
                     /locus_tag="PKJ65_01645"
     CDS             complement(3994..5049)
                     /locus_tag="PKJ65_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40574.1"
                     /translation="MQLKPTISETASEMNKTYSDALKHAVGNVTFRALPPSDISRYLN
                     LSSYKYDETDLDKYVDDLLLGHFAMMEARSEVCDNYIPQLNIDLGIGDYAAFVTGDIY
                     FKEDTSWTVPTLHDLKMYRDLAPIGTSKWYRKYMYILEKVLEKTKDTDIPFARGFFSP
                     LDLAESLRGTDIYTDFYDYPDDVHDFLDYCAQATIKFAEDIYGVIKKYRKNAQYGTMF
                     IDGIVNMSEDIASMISPALYDEFCAPHTQKIIDHFGSGHMHSHSCAMYLVKHICSLNN
                     VANLWLATDPNHPRPFDMLEQLIEDADGTCLAIDCKSFKEIEDNIDILRKGNFSICLP
                     VETMEDAVYFAKRFREL"
     gene            complement(5143..>5717)
                     /locus_tag="PKJ65_01650"
     CDS             complement(5143..>5717)
                     /locus_tag="PKJ65_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40575.1"
                     /translation="RQNNYMDLAYDLLLAINVSEEHIRNIGMNTYSPMMLYDSMYSDS
                     LFFKIINDYPKAVEKAKATFKNDKECVLIYSIRNNKGINTLIDNIPLNKIAFTGTNRK
                     EIDEYIYLNKDKNFIAIGDIAGFIALSNISYEQLALITFSNNIAFPDAVSQITFNNQE
                     LKDNIKRIFEKLDARQASEQEQHIFRINPN"
BASE COUNT         1730 a         1201 c          973 g         1813 t
ORIGIN      
        1 taatcatgaa tatagaacca gtctttatac tgtgagtttt gacctttttc cagcacatca
       61 cgaaatattt tgttattgtt gctcatgtga ttgaaaacgc agtccaacac tatccgcatg
      121 ccttttttgt gaatactttg tataagattg cttaattctt ttcttccgcc gaacatctca
      181 tcaatggagt agtagtcttc aatgtcgtat ttatgatttg tagcggaact gaaaatcggg
      241 cttaaatata ctatatcgat acctaaagac tttatataat caagcttgta ttcaatacct
      301 ttaaaatccc ctccgaaaaa gtgacttcgc aaaggtttat cgttccaatc cgcattgaca
      361 aagtctttgt cacaggaact gttaaatctg tccacaaaaa tctgataaac cgttgcccct
      421 tcagcccatt taggcaactt gtaaatctca tcgtcgttta taacggggaa aaagaaactg
      481 ttttcattct tcacttcatt gcccataaaa ccgtcggatg tataaaaaac ctgctctttt
      541 tcaccgataa gttcaaaaaa gtatttgaag tgtttctcct tctggcttat ctttgcttcg
      601 tataaatcaa aatgttcatc ctgcagtatt tttgccatgt tttctttttt tctgttagtt
      661 gagtgatcat acacattcat ataatgcaca atgacactct tgcagtcgtt tttagctgtt
      721 ctcagtcgta tacataaagt atgtgaatca atagcatacg catatgttcc gctgtttata
      781 tgtaagaccg catatctgtt tataacaccg ctcattgttt gacacctcct tcggtaagac
      841 cgccgataaa gtatctttgg aaggaataaa acagtataaa tatgggcaga ctggctaaaa
      901 cggcgcctgc cgagtacatt ccccagttct gctgaaattg accgtaaatg aggctttgca
      961 gacccaccgc taccgtcatt ttctccgcac ctgaagttat aagcatactg gctatggcat
     1021 attcattgta ggtaagtaca aaactttgaa tggctacaat tcccagaatg ggttttacca
     1081 acggcaatgt aatatggatg agcacctgaa atctgtttgc gccgtctatc attgcagctt
     1141 cgtctatttc atacggaatc gtatcaaagt agcctttgga cacaagaata agccctgctg
     1201 tcgctataac ggaatctatt atcaccaacc ctatcagttt gttgagtaat tccaaagccg
     1261 caaatatctt aaacagtgag accagcataa gagtaagcgg gaatatctga atgagaagca
     1321 ttgtccggaa taatgcttct cttccggcaa acttatactt ggaaaataca ttggatgcaa
     1381 ccgacaccat aacaagcgat atgagcatgg tagctaaaga cacaagaagc gaattaccca
     1441 gccagtgaag gtattcggtc tcggtaaaaa gtttttcata attatctacg gaaaaaccca
     1501 tatcgccgta tcccgtaata tccagcaggt cttttccgaa ggatattaga aggatatata
     1561 caaacggaat aagtacaatc aatgctaaaa caatcaaaat tgcatgaaca ccgatatttc
     1621 ggaacaactt attcttttta cgcattttcc gtcacttcct ttcatggatt tttcttattt
     1681 cccagcttca tcgttatcag tgtaaatacc gcgacaaaga ggaatattat tactgaatat
     1741 gctgctgcaa gaccgtagtt cttggtattg taagcaaggt tgtacacgta ggtaataagg
     1801 agatcggtag caccgggtgc tcccaaagta cttccgaaag gacctcccga agtaagcaga
     1861 tacaccccga aattgttaaa ctgcattata aatcccatta tcagtgtagg tagcaaagac
     1921 cggaagataa tgggaacggt tattatgaag aataccttta ctcttcccgc accttcaatc
     1981 attgcggctt catagaaatc cttaggtatg gattgtaaat accccaaagc taaaaacata
     2041 taatacggag tgcccatcca ggatacaaca aaaattgtac agactcttgc cagaaaagga
     2101 ttatccagcc acataacttt acctatccct aaaaggctca gcaaattgtt caaagctccg
     2161 ccgtccgttg ccagtatccc ctgccatgta agtaaagtta taacactggg aataacccac
     2221 ggcagtatga atatcatttt gtatatttta gatacctttg cttttctttt ttccagtaaa
     2281 gatgccaaca tggtaccgcc cagaaaagtt ataaccatta cgcttgtggc aaagattaca
     2341 gtccatatga acattgaaac aaatccgtta agaccttcaa tgaaaatggt tttgtagtta
     2401 tgtccgccga tgaatacata gtctatcaga tttaacgagc tgtaattggt cattgacagg
     2461 tagatattat gaacaatggg aaacaggtag aatatcccta taagtaccag tacgggaaaa
     2521 agagcgatat aagaaacaac cgttgctctt aacattatgc ggttggccga tgcactgtaa
     2581 cctttccttc tccttataaa agtgataatc aaaatagcaa ggagtattac aaaaaacagt
     2641 ccgatgaaaa cataccctaa aacaggtatt tccacctttt ccacctgaac attcattttt
     2701 tttacatcat ttaatatggc tttcgacagc tcttccagca tactgtcgga atctacggga
     2761 ttaccattta tatccccata gaaaatcgat tgatatgcct tatcggtgta gtggtaccat
     2821 atagctcctt cgggaatatt gggaacaggc tcaatgttct cacatatgtc ggccattaca
     2881 tcatacattt cctcactctt aatgtagttg ctttccctga ccgaaagatt taccggaaaa
     2941 cggcttccaa cctttttctc aaaattattt cccgcttcaa taagcatttg ttggtattca
     3001 tccctacaca tgaattccaa aaacttttta gcttcatccg gatagtcgga atgtgcattt
     3061 atcacaaaac cgtttacggt tgcaaacggt gatgtagccg ttttgttatt atttaaagga
     3121 agaggagcga aaccgaaatt gacacctttt tgtctgtaga aagaattctg ccaaaatccg
     3181 ttgattataa tagcaacatc ctgatttgca aaagccgccg acacatcact gtccgtcccg
     3241 ttgcggcttt tcagaacaag ctcatggtcc aacagatttt taatcttttc cacatatttt
     3301 tttgaccctt cattgctcaa acccacatca aacggattgt aattaccgtt ttcgtcaact
     3361 ccgtaataat atcctccgta gtttttaata ataggataca tgaaatacat gttcttggga
     3421 tttaaaacaa atgctttcat agggtttccg ttgtgagttg ttgttccgaa atctattgca
     3481 tactgaaaaa actcatccca tgtaagattt gcaatctgtt ctttggatat aacatcacgg
     3541 ttataaacca atccgtatgt ctctaccgaa tatcctatgc cgtattgctt atctcgcact
     3601 tcaaaagaag taaatgccga ctcatgatat gccaatctaa actcttcggt aaaatccaac
     3661 tccagcagga aaccgtttac cgcaagatct cctatatcgg catgctgtat cattactata
     3721 tcgggttttt caccggtctt ggcattgttt accaaatctc tgggagcgtc aaacaaactt
     3781 cttctgtcta taaccgatac tgttattccc gtttctattt tgaattgctg ggcaatctca
     3841 cgtattacat cattctcgcg ctttatttca ctgccccaaa cggtaatcac cttatctccg
     3901 tcggctttga caatattaca gaagcatacc gaaagtatga tggctactgt tagtattgct
     3961 aagacagttc tttttctcat tgtaatcccc caattacaat tcgcgaaaac gtttcgcaaa
     4021 atataccgcg tcttccatag tttctacagg cagacatatg gaaaaattac cttttcttaa
     4081 aatatctata ttatcttcta tttctttaaa tgatttacaa tctatcgcaa ggcaggttcc
     4141 gtccgcatct tcaatcagtt gctccagcat atcaaaaggt ctgggatgat tgggatcggt
     4201 tgccaaccag aggttggcta cattatttag cgagcaaata tgcttaacaa gatacattgc
     4261 acaactgtga ctgtgcatat gtccgctccc gaaatggtca atgattttct gtgtatgcgg
     4321 agcacagaat tcgtcataaa gtgcgggact tatcatgctg gctatgtctt ccgacatatt
     4381 gacaatcccg tcaataaaca tggtaccgta ttgagcattc tttctgtatt tctttataac
     4441 accatatata tcttccgcaa acttaattgt agcttgagcg cagtaatcca aaaaatcgtg
     4501 aacatcatcg ggataatcat aaaaatcggt atatatatcc gttcctctca atgattccgc
     4561 aaggtctaaa ggtgaaaaga atcctcttgc aaaaggaatg tctgtatctt ttgtcttttc
     4621 caacaccttc tccaagatat acatatattt tctataccac ttagatgttc ctataggagc
     4681 caaatcccta tacattttca aatcatgaag tgtaggtacc gtccagcttg tatcttcttt
     4741 gaaataaata tctccggtta caaatgctgc ataatcacct attcccagat caatatttaa
     4801 ttgcgggata tagttgtcgc aaacttcgct tctggcctcc atcatggcaa aatgaccgag
     4861 aagtaaatca tcaacatatt tgtctaaatc ggtctcatca tatttataag aagaaagatt
     4921 aagataccgg gaaatatcac tcggcggcaa tgctctgaat gtgacattac cgactgcatg
     4981 cttaagcgca tcagagtatg tcttattcat ttctgacgct gtttcggaaa tagtcggttt
     5041 tagttgcatt atatgcatca ttgactcatt acctctttgg ttaaccgatt aagcaatcaa
     5101 aaaatatcac ataaagagta taatgtcaac acatttttta attcagttgg gatttatccg
     5161 gaatatatgt tgttcctgtt cacttgcctg tcttgcatcc agcttttcga agattctttt
     5221 aatattgtcc ttcagttcct gattgttgaa ggtaatctgc gagaccgcat cgggaaatgc
     5281 gatgttgttg gaaaaagtta taagagccag ttgctcgtat gatatgttgg ataatgcaat
     5341 aaagcctgca atgtctccta tggcaataaa attcttatcc ttgtttaagt aaatatattc
     5401 atcaatttct tttctgtttg ttcccgtaaa agcaatctta ttcaaaggaa tattgtcaat
     5461 caaagtatta atgcctttat tattccgaat actgtatatc aacacacatt ctttatcatt
     5521 tttgaaagtt gctttagctt tttccaccgc tttgggatag tcattgatta ttttaaagaa
     5581 aagactgtct gaatacatac tatcatacag catcatagga gaatatgtat tcatacctat
     5641 attacgaata tgctcttcac ttacattgat ggcaagaagt aaatcatatg ccagatccat
     5701 ataattattc tggcgtt
//
LOCUS       DAOPWC010000042         5715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14037_length_5715_cov_3.326678, whole genome shotgun sequence.
ACCESSION   DAOPWC010000042 DAOPWC010000000
VERSION     DAOPWC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14037_length_5715_cov_3.326678"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKJ65_01655"
     CDS             complement(<1..372)
                     /locus_tag="PKJ65_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNZ40576.1"
                     /translation="MEYIKGSITAPVGFRANGIHCGIRKNKTKRDLALIVSDTICTAA
                     CVYTKNLVKGAPITVTQQNVADGYAKAIICNSGNANTCNENGMEIARGMCNLAAKELN
                     INPADVIVASTGVIGQQLSLDP"
     gene            complement(388..1317)
                     /gene="argC"
                     /locus_tag="PKJ65_01660"
     CDS             complement(388..1317)
                     /gene="argC"
                     /locus_tag="PKJ65_01660"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNZ40577.1"
                     /translation="MVKVFIDGSYGTTGLRIHERLASRADIQLIILPEQQRKDVNKRA
                     KALNSADIAFLCLPDQAAIESVSLINNPDTVVIDASTAHRTDPDWTYGFLEMGEDIYD
                     KVKQSKRICVPGCHATGFTALVKPLIENRIVQKNYPFSCTSLTGYSGGGKAMISDYTD
                     KNRDSSLSVPRIYALTQTHKHLKEMRHYTGIVDYPHFNPVVCDIYSMMCVTVNISVKG
                     VKIDDICDIFVKKYGNRPLFDISDIEGESYNGYIPCDDFKDKDSIKIVVTGNDERISL
                     IALYDNLGKGASGAAVQILNVITGKEETYGLKV"
     gene            complement(1310..2686)
                     /gene="argH"
                     /locus_tag="PKJ65_01665"
     CDS             complement(1310..2686)
                     /gene="argH"
                     /locus_tag="PKJ65_01665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNZ40578.1"
                     /translation="MEKLWKGRLNSDTDKLADKFNSSIDIDHILYKQDITCSIAHAEM
                     LGATGIIPFSDAKKISECLKEILKEIDDGSLDIDRSFEDIHTFVEYELIKRLQDTGKM
                     LHTARSRNDQVATDFKMYVKERICDITGYIKELLSVLADHASDNLDTVMPGYTHLQRA
                     QPVTLGHYLMAYAMMFHRDLQRYRSFHSMMNTCPLGSGALAGTTYNTDRHMTAAALGF
                     EMPSLNSIDGVSDRDFATDACYANSMVMMHLSRFCEELILWASCEFKFIEISDGFSTG
                     SSIMPQKKNPDIAELIRGKSARTFSDLNTLLVMQKSLPLAYNKDMQEDKEAVFDSFDT
                     VSACLKVFTAMLKETVFLKDNMKKAAHKGFINATDCADYLVKKSLPFRDAYKITGQLV
                     AYCIQNGYTLETLPLEIYKSRSSLFEHDVYKEISSDACVEKRISFGGPSAQSVLLQIE
                     HIREAVNG"
     gene            complement(2697..3935)
                     /locus_tag="PKJ65_01670"
     CDS             complement(2697..3935)
                     /locus_tag="PKJ65_01670"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNZ40579.1"
                     /translation="MNKFDEKIKKIVLAYSGGLDTSVIIPWLKENYEGCEVIAVCGDV
                     GQGAETEGLEERALATGASKLYIEDLTEEFVYDFIFPTLKAGAVYEKKYLLGTSFARP
                     VTAKRIAEIALKENADAICHGCTGKGNDQVRFELTIKAFAPKMKIIAPWRIWDIKSRN
                     QEIDYAIAHNVPLKVSRETSYSKDKNLWHLSHEGLDLEDPSNEPQYNKEGFLEMCVSP
                     EQAPDKAEYITVDFEKGIPVAVNGNKMSGPDVIKTLNTIGGKHGIGLTDMVENRLVGM
                     KSRGVYETPGGTILYHAHAVLESLTLDRDTSHYKALVAEKFAELVYFGQWYTPLREAL
                     SAFVDKTQENVTGQVKLKLYKGNIINAGTVSDYSLYNMDFATFEEDNVYNQKDAEGFI
                     NLFGLPIKVKALADGKKLNK"
     gene            4285..4776
                     /locus_tag="PKJ65_01675"
     CDS             4285..4776
                     /locus_tag="PKJ65_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ40580.1"
                     /translation="MRTLGNRIAELRRAKNITQDEMAEKLNVTAQAVSKWENDISCPD
                     IMLLPKIAQMLSVTVDELLSDQPKKSVELVPEEKRKSIDDLVLRIYVNSKDGDRVKVN
                     LPMSLVKAGLEMGLKMPEVSGNEALKDIDFSQLIALADKGVIGKLVEVESADGDTVDI
                     VVE"
     gene            4778..5035
                     /locus_tag="PKJ65_01680"
     CDS             4778..5035
                     /locus_tag="PKJ65_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40581.1"
                     /translation="MRIKVKSDDGKKINLIFPTWFVFSDLGARIAAKVISRNAEPHEF
                     NLSGKDLSRLMTEVRKIKNKYGRFELVDVQSAEGDIVKIVL"
     gene            5047..>5715
                     /locus_tag="PKJ65_01685"
     CDS             5047..>5715
                     /locus_tag="PKJ65_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 2 glycosyl transferase"
                     /protein_id="HNZ40582.1"
                     /translation="MRKKRLRFTILLLICLQMFVLWNNAFTCHASDDFVYRRNKSMLF
                     EEAGLKYPAKVSGRDFLIYTNGQWKKVFLKGVNIGAAKPGTFPGELAITKEEYLRWFR
                     YISRMNAEVIRVYTTMKPDFYDALLEFNKTSSKPLYLMHGVWVNEEVIAMLNDAYADN
                     ERIKNDFIKDALDLVDIFHGNAELPFKYGFAHGKYETDISKYVIGWILGIEWDPYFVL
                     GTNEN"
BASE COUNT         1546 a         1187 c         1223 g         1759 t
ORIGIN      
        1 agggtctaat gacagttgct gccctataac cccggtggat gccactatta catcggcagg
       61 attaatgttt aactcctttg ccgcgagatt acacataccc cgtgctatct ccataccgtt
      121 ttcattgcag gtattggcat tgccgctgtt gcatatgatg gcttttgcat atccgtctgc
      181 aacattctgt tgtgtgaccg ttattggtgc tccctttaca agattttttg tatagacgca
      241 agctgcggta catatagtat cggaaacaat aagggcgaga tcccttttgg tcttattctt
      301 ccttataccg cagtgtatac cgtttgctct gaacccgacg ggtgcggtta ttgaaccttt
      361 tatatattcc attcttttat ctctccttta taccttcaaa ccgtaagttt cttctttacc
      421 ggttatgaca ttcaatatct gtaccgccgc acccgatgcc cctttaccga gattgtcgta
      481 aagtgcgatt agcgatattc tttcatcgtt tcccgtaaca actattttta ttgaatcttt
      541 atctttgaaa tcatcacaag gtatgtagcc gttatagctt tctccttcta tgtccgaaat
      601 atcaaacagc ggtctgttgc cgtatttctt tacaaaaata tcgcatatat cgtcaatctt
      661 tacacccttt acggatatat tgaccgttac acacatcatg ctgtatatgt cgcaaacaac
      721 gggattgaaa tgaggatagt caactattcc ggtataatgc ctcatttcct tcagatgctt
      781 atgcgtctgt gtaagtgcat atatgcgcgg taccgacagc gatgagtctc tgtttttgtc
      841 ggtataatcg cttatcatgg cttttcctcc gcccgaataa ccggtgaggg aagtgcagga
      901 aaaaggatag ttcttttgta caatgcggtt ttctatcagc ggctttacca atgctgtaaa
      961 tcccgtagca tggcaaccgg gaacacagat gcgtttgctt tgcttaactt tatcgtatat
     1021 atcctctccc atttccaaaa agccgtatgt ccagtcggga tcggttctgt gagcggtgga
     1081 agcatctatt accacggtat cggggttgtt aataagggag accgactcta tagccgcctg
     1141 atcgggaaga cataaaaaag ctatgtcggc actgtttaag gcttttgctc ttttgttgac
     1201 atctttcctt tgctgttcgg gaaggataat gagctgtata tccgcccttg atgcaagcct
     1261 ttcatgtatt cttaatcccg tggtgccgta agagccgtca atgaaaacct taaccattta
     1321 cagcctccct gatatgctct atctgtaaaa gtaccgattg tgccgaagga cctccgaaag
     1381 atatcctttt ttccacacat gcatccgatg atatctcctt atacacatcg tgctcgaaca
     1441 aagaagagcg gcttttgtaa atctccaaag gcagagtttc cagtgtataa ccgttttgta
     1501 tgcaataagc cactaattgt cccgtaattt tgtatgcatc ccgaaaaggc agagatttct
     1561 ttaccagata atcggcacag tcggttgcat ttatgaaccc tttatgtgcg gcttttttca
     1621 tattgtcttt aaggaagacc gtctctttaa gcatagcggt aaatactttc agacaggcgc
     1681 ttacggtatc gaaactgtcg aataccgcct ctttgtcttc ctgcatatcc ttgttgtatg
     1741 ccaaaggcaa ggatttttgc ataaccagca gagtattcag atcggaaaaa gtccttgcac
     1801 tctttccgcg tataagctcc gctatatcgg ggtttttctt ctgaggcatt atggaagagc
     1861 cggtggaaaa accgtcggag atttctataa acttaaactc gcacgaagcc cacagtataa
     1921 gttcttcgca gaaacgggaa aggtgcatca ttaccatact gttggcataa caggcgtctg
     1981 tcgcgaaatc gcggtcggat acgccgtcaa tactgttgag tgaaggcatt tcaaagccta
     2041 aagccgcagc ggtcatatgt ctgtcggtgt tatatgtggt tccggcaagg gcaccgctac
     2101 ccaaaggaca ggtattcatc atggaatgaa aagatcggta cctttgtaaa tcacggtgaa
     2161 acatcattgc atatgccata aggtagtgac ccaatgtaac cggttgtgct ctttgtaaat
     2221 gggtatagcc gggcattacg gtatccaggt tatccgatgc atgatccgcc aacacgctca
     2281 acaattcttt tatgtaccct gtaatatcac atatccgctc tttcacatac atcttgaaat
     2341 cggtggcaac ctgatcgttt ctggaacgtg cagtatgcag cattttaccg gtatcttgca
     2401 atctttttat aagttcatat tccacaaagg tatgtatatc ttcaaaactc ctatctatat
     2461 ccagactgcc gtcatctatt tcttttaata tttctttaag gcattcggat attttctttg
     2521 catcggaaaa aggtatgata cctgttgctc ctaacatttc cgcatgagct atcgagcatg
     2581 ttatgtcctg tttgtatagg atatggtcaa tatctatgga agaattgaac ttatccgcca
     2641 gtttgtccgt atcgctgttt aaacgtcctt tccaaagctt ttccatttta acctctttac
     2701 ttatttaatt ttttgccgtc ggcaagcgct tttaccttta tgggcagtcc gaacagattt
     2761 atgaaacctt cggcatcttt ctggttgtat acattgtctt cttcgaatgt ggcaaaatcc
     2821 atattgtaca gggaatagtc ggaaaccgtt cccgcattga ttatattgcc cttatacagt
     2881 ttgagtttta cctgtcccgt tacattttcc tgagtcttgt ctacaaatgc cgacaaagct
     2941 tctcttaatg gggtgtacca ctggccgaaa tataccagct ctgcgaattt ctccgccaca
     3001 agcgccttgt agtgcgaggt gtcgcggtct aaagtgagcg attccagcac tgcatgtgca
     3061 tgatacagta tggttcctcc cggtgtctcg tatacgcctc tggatttcat tcccacaaga
     3121 cggttttcca ccatatccgt caatcctatt ccgtgctttc cgcctattgt attgagtgtt
     3181 tttattacat ccggtccgct catcttgttg ccgtttaccg ctaccggaat tcctttttca
     3241 aaatctaccg ttatatattc cgctttatcg ggagcctgtt ccggagagac gcacatctcc
     3301 agaaaaccct ccttgttata ctgaggctca ttggagggat cttccagatc cagtccttca
     3361 tgcgaaagat gccagagatt cttatctttg gagtagctgg tttctctgga taccttaagc
     3421 gggacattat gcgctatggc ataatctatt tcctggttcc tcgactttat gtcccagatt
     3481 ctccatggtg ctattatctt catcttaggt gcaaaggctt ttatggtcag ctcgaagcgt
     3541 acctggtcgt tgccttttcc ggtacagccg tgacatatgg catcggcatt ttcctttaat
     3601 gcaatttccg ctattctctt tgcagtaacc gggcgcgcaa aagatgtgcc caataaatat
     3661 ttcttctcat acaccgcacc ggcttttaag gtggggaata tgaagtcata tacaaactct
     3721 tcggtaagat cttctatata cagttttgaa gctcctgtag caagtgctct ttcttccagt
     3781 ccttcggttt cggctccttg tccgacatct ccgcagactg ctattacttc gcagccttca
     3841 tagttctctt tcagccacgg tattattacc gatgtgtcca atcctcccga atatgccagt
     3901 actattttct ttattttctc atcaaattta ttcattgcac agcctctttc tgtttgattg
     3961 gcataattat acaggattac tgcataaagt caatacattt atgaataaaa tatcttaata
     4021 tatgcataaa tatgaatatt cggtgaatat taatcgaatg taagcattca tgaagagggg
     4081 atagtgctgc aaaattacca aaatcaaccg ttagttgagt aattatacaa ccgcaggttg
     4141 tgaaaaaaca gtaaaaacaa cctttgatat attcaatacc tgtataggcg atactaaaat
     4201 ttaattatga aaagtgccgg tattcagctt tgtaagtgtt gaatatgggc aaagaaaaat
     4261 atatattata taagggaaga aaagatgaga acattaggaa acagaattgc ggaattaaga
     4321 agagcaaaga acatcactca agatgagatg gcggagaagc tcaatgtaac agctcaggcg
     4381 gtatccaaat gggagaacga tatatcctgt ccggatatta tgctgttgcc caagatagca
     4441 caaatgctgt cggttacggt ggacgagttg ttgtccgatc agccaaagaa aagtgtggaa
     4501 cttgtaccgg aagaaaagag aaagagtata gacgatctgg tattaaggat ttatgtcaac
     4561 tccaaagacg gtgacagggt aaaagtgaac ctgcccatgt cgctggttaa agcgggactg
     4621 gaaatgggac tcaagatgcc ggaagtttcg ggaaatgaag cattgaaaga catagatttt
     4681 tcccaactga ttgcgctggc ggataaaggt gtgataggta aacttgtgga agtggaaagt
     4741 gcggacggag ataccgtgga tatagtggtg gagtaatatg cgaatcaagg ttaaatcgga
     4801 tgacggtaaa aagataaatc ttattttccc cacatggttt gttttttccg acttaggtgc
     4861 cagaatagcc gctaaagtca tcagcaggaa tgccgagccg cacgagttta atctatccgg
     4921 taaggattta agcaggctga tgactgaagt gaggaagatt aaaaataaat acggacggtt
     4981 tgaactggtg gatgtacaaa gtgccgaagg agacattgtt aagatagtgc tgtgataaaa
     5041 ggtgcaatga gaaaaaaaag attaagattt actatattac tactcatatg tttacagatg
     5101 tttgtattgt ggaataatgc ttttacctgc catgcatcgg atgattttgt ttatcgcagg
     5161 aataagagca tgctctttga ggaagcgggg ttgaagtatc ccgccaaggt ctccggtcgt
     5221 gactttctta tatatacaaa cggacaatgg aagaaggttt ttttaaaagg tgtaaacatc
     5281 ggagcggcaa agcccggaac atttcccgga gaactggcca ttaccaaaga agagtattta
     5341 cggtggttta gatacataag caggatgaat gcggaagtta taagggtgta tacgacaatg
     5401 aagccggatt tttacgatgc attgcttgaa ttcaacaaaa cctcctccaa gcctctgtat
     5461 cttatgcacg gtgtatgggt gaatgaggaa gttattgcta tgctgaatga tgcctatgcc
     5521 gataatgaaa gaataaaaaa cgactttatt aaagatgcct tggatttggt ggatattttc
     5581 cacggaaatg ccgagctgcc ttttaagtac ggatttgcac acggaaagta tgaaaccgat
     5641 atttcaaagt atgtgatagg ctggatattg ggaatcgaat gggatccgta ttttgtttta
     5701 gggaccaatg aaaac
//
LOCUS       DAOPWC010000043         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14224_length_5665_cov_3.167736, whole genome shotgun sequence.
ACCESSION   DAOPWC010000043 DAOPWC010000000
VERSION     DAOPWC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14224_length_5665_cov_3.167736"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..366)
                     /gene="rpmF"
                     /locus_tag="PKJ65_01690"
     CDS             complement(181..366)
                     /gene="rpmF"
                     /locus_tag="PKJ65_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNZ40583.1"
                     /translation="MAVPKRKVSKQRQRKRRTFYKLEEPNLVRCSQCHTLKRQHRVCD
                     ECGYYDGKEVIKKEEEK"
     gene            complement(391..900)
                     /locus_tag="PKJ65_01695"
     CDS             complement(391..900)
                     /locus_tag="PKJ65_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNZ40584.1"
                     /translation="MKIDITDIIERDGAVKQFATDINKLSDRNTGSGKAIVDVPGVIH
                     IEGEIYNHEGILDISAQIFGQLEAQCYRCLEDITAPFSVHMEETFERANGSKNPDNYE
                     YSGRILDISQAVIDSVYLNIPDRMLCKSNCKGLCPKCGSNLNKKQCGCIEQSEENGRF
                     SALKDLFKE"
     gene            complement(974..2176)
                     /locus_tag="PKJ65_01700"
     CDS             complement(974..2176)
                     /locus_tag="PKJ65_01700"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNZ40585.1"
                     /translation="MKVLVINTGSSSLKYQMIDSENEKMLAKGLCERIGLPNGIHTYT
                     CADGSSSTDECVISDHSHAFKLMLDALVCPGKGVLKSISEIDAVGHRIVHGGERFTKP
                     ALIDEDVIKAIEDCVDLAPLHNPAGILGIRVCQKVLKNVPMVAVFDTAFHHTIPDYAY
                     MYAIPYEMYEKYRVRKYGFHGTNHKYVAQRAAELMDRPLDKIKIVSCHLGSGASICAI
                     DRGKSIDTSMGFTPLSGLAMGTRSGTIDPSIISFIMEKKNMTVEEVNYYLNNKSGLLG
                     VSGISSDFRDLVKARNEGNKRAALALAIFAYRVKRYVCEYVGVMGGIDALVFTAGVGE
                     NNAYIRELITKNLDIFGIKMDRRKNNNVKGETDVSRDRSNVRILIIPANEELEIARQC
                     KSLLEDGK"
     gene            complement(2202..3194)
                     /gene="pta"
                     /locus_tag="PKJ65_01705"
     CDS             complement(2202..3194)
                     /gene="pta"
                     /locus_tag="PKJ65_01705"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNZ40586.1"
                     /translation="MTFLERTIQRAKSNKKTIVLPESEDIRTLKAASVALKEGIADII
                     LLGNKEKVKKNGKGLNIPEEKILEIDKFDRIDEYANMLYQLRKEKGVTLDIAYKLLEN
                     PLYFAVMMVKMGDADGMVAGAVSSTSDTLRPALQILRTAKGTKIVSAFFIMCVPDCQY
                     GANGVFAYGDSGLVENPDAEQLSEIAISTAKSFELLVEEQPVVAMLSYSTYGSAKSEL
                     TEKVIQATKLAKEKAPELLLDGELQVDAAVVKEIGQRKAPGSSVAGKANVMIFPNLDA
                     GNIAYKLTERLAKAEAYGPLLQGIAKPVNDLSRGCTAEDIVGVIAITAVQAQSV"
     gene            complement(3346..4551)
                     /locus_tag="PKJ65_01710"
     CDS             complement(3346..4551)
                     /locus_tag="PKJ65_01710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ40587.1"
                     /translation="MAVAKGKKEDVKKKSSKEISKEETIIRYGFLTLAVIIGLCLIYF
                     VIASLIPNNYIKIGNHKIAEEEFMYQTNTQYKVFANYYQDYIRNLGFDLSTPEGISAF
                     MKTAYDNQRSFGQMLLEGVVDSIQEQYIMLDQIKETGFKIDEEELEESLAIQLEAIQR
                     AADESGYDLDKYAKLFYGTSFKAMQKVIRQTLRAQQYTTYLEEQNISAVTEERALSYY
                     NSVDAEGNAVRDEIDRFTVVTILKRTVDDQFQPLAEDVIAAAKVKAAEIFEMAKSGGD
                     IYELAKLYSDDLNDQEAEESEEETDDAAKPGEFTFTKKEVGTTELSKWALKASVGDLD
                     LIETEIGHVIIKLIDRTDYEDMKDDIFKLIASSDFEMKMNEYKDMEQYKVGLYKPYQE
                     LYLTYAITQ"
     gene            complement(4633..>5665)
                     /locus_tag="PKJ65_01715"
     CDS             complement(4633..>5665)
                     /locus_tag="PKJ65_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ40588.1"
                     /translation="ILREKDRNGQVFYLYNRVMDMDSKFYHLKTLLKDQVRICVAHGQ
                     MPEKEFENTIIKFINREYDVLLCTTIIEAGIDISNANTLIIEDADKLGLAQLYQIKGR
                     VGRSDRVAYAYITYKRGKELTEEASKRLQTIKEYTEFGSGIKIAMKDLEIRGGGDLLG
                     ANQSGHMQGVGYDMYVRILSEEIANLRGEKVKGAVTETSVDLNISAYIDKSYISDDLS
                     RLKMYKRIATVATVTEKEDIIDELTDRYNQPPGHVVNIINVAYAKNMACGLVISEIVQ
                     TNKGFVMIYEKEHAIHFRKITDVLDKERIKYTVKAQEDLQIRVDTDKKNEELLDFITK
                     ILEKAVESK"
BASE COUNT         1387 a         1375 c          914 g         1989 t
ORIGIN      
        1 catcggaaat atggtactgg taatcgaaaa tatcctttat tccgaaaccg tttaccgtaa
       61 gcaaaaaatc gccgggtttt ataccggctc tcttcgctaa acttcctttc aatatctgtt
      121 taattgcaat tgccttgttt tccatatagc aaaaaggcga tatatcatcg ccttataaca
      181 ttacttttcc tcttcttttt ttattacttc ttttccgtca taatatccgc attcgtcaca
      241 tactctgtgt tgacgtttga gagtatgaca ctgtgaacat ctgacaagat tgggctcttc
      301 cagtttatag aaagtccttc ttttacgttg tctttgtttt gatactttac gcttgggtac
      361 agccatttta cacctcctgc attactaaca ttattcttta aataaatcct tcaaagcgga
      421 aaatctcccg ttctcttcgc tttgttcaat gcatccgcat tgctttttgt tgagattgct
      481 tccgcattta gggcacaatc ccttacaatt actcttacac aacattctgt cggggatatt
      541 taaatatacg gaatcgatta ccgcctggga gatatccagt atacgaccgg aatactcgta
      601 gttatccggg ttttttgaac cgttcgcgcg ttcaaaagtc tcttccatat gcacagagaa
      661 aggggcggta atgtcttcaa ggcatctgta gcattgcgct tccaactgcc cgaagatttg
      721 tgccgaaatg tccaggatgc cttcatggtt gtatatctca ccttcaatgt gaataacacc
      781 cggtacatca acaattgcct taccgctgcc ggtattgcgg tcactcaact tatttatatc
      841 ggttgcaaat tgctttacag caccgtctct ctctataata tcggttatgt caatcttcat
      901 ttaatacctt tcaaaaaaac cgacaagtgc tattatacgg ttacctctta tgccttgtca
      961 agacaatttt cctttactta ccgtcttcca acaggctttt acactgtctg gctatctcca
     1021 attcctcatt tgccggaatt atcagtatcc ttacattgga ccggtctctg cttacatcgg
     1081 tttccccttt tacattatta ttctttcttc tgtccatttt tatgccgaat atatcaagat
     1141 tcttggttat aagctctctt atgtatgcat tgttttcacc cacacctgcg gtaaatacca
     1201 aagcatctat tcctcccatt actcccacat attcacatac atatctttta actctgtaag
     1261 caaatatggc cagtgccagt gcagctcttt tattgccttc gtttcttgct ttgacaagat
     1321 cccgaaaatc actgcttatt cccgatactc ccaataagcc ggatttattg tttaaataat
     1381 aattgacttc ctccaccgtc atattcttct tttccattat gaaggagatt atggacggat
     1441 ctatggtgcc gcttctggtt cccatagcca atcccgaaag cggtgtgaat cccatggaag
     1501 tatctatgga ttttcctctg tcaatagcac atatggatgc tccgcttccc agatggcagg
     1561 atactatttt tattttatcc agaggtctgt ccattaattc cgccgctctc tgagccacat
     1621 acttgtgatt ggtgccgtgg aatccgtatt tacgtactct gtatttctca tacatttcat
     1681 aaggaatagc gtacatatat gcataatcgg gtatggtatg atgaaatgcg gtatcaaaaa
     1741 cagccaccat aggcacattt ttcaatacct tctgacacac tcttattccc aatatccccg
     1801 cagggttgtg taacggtgcc aggtccacgc aatcctctat cgcctttatt acatcctcat
     1861 ctattaaagc gggcttggta aatcgctcac cgccatgtac tatcctgtgc cctaccgcat
     1921 ctatttccga tatgctcttc aatactcctt ttccgggaca tactaaagca tccagcatta
     1981 acttaaatgc gtgggaatgg tcacttatca cgcattcatc ggtacttgat gagccgtccg
     2041 cacatgtata tgtatgaata ccgtttggca gccctattct ttcacataac ccttttgcca
     2101 gcattttttc attttcgctg tcaatcatct gatacttcaa tgaagaactg cctgtattta
     2161 ttactagtac tttcatatct acctgacctt aatatattaa attaaacact ctgggcctga
     2221 accgccgtta ttgcaataac tcccactata tcttccgccg tgcagcctct ggataaatcg
     2281 ttaaccggtt tggctattcc ctgaagcaga ggaccgtaag cttccgcctt tgccagtctt
     2341 tcggttaatt tatatgctat attgcccgca tccagattgg gaaatatcat tacgtttgct
     2401 tttcccgcaa cagaacttcc cggtgctttt ctctgaccta tctcttttac tacggctgcg
     2461 tctacctgca gttcgccgtc cagcagcagc tcgggagctt tttcctttgc caatttggta
     2521 gcctgaataa ctttctcggt caattcggat tttgcacttc cgtatgttga ataactgagc
     2581 attgccacaa ccggctgttc ttccactagc agctcaaaag attttgcagt ggaaatggct
     2641 atctcggaaa gttgctccgc atcgggattt tccaccaaac cggaatctcc gtatgcaaac
     2701 acaccgtttg ctccgtattg gcagtcgggt acacacatta tgaaaaatgc actgactatc
     2761 ttggttcctt ttgccgttct cagtatctga agagccggcc ttaaggtatc acttgtagag
     2821 cttaccgcac ctgccaccat tccgtctgca tcgcccattt ttaccatcat aacggcaaag
     2881 tataacggat tctccaatag cttatatgct atatccagtg taacaccctt ttcttttctc
     2941 agctgataca gcatatttgc atattcatca attctgtcga atttatctat ttccaatatc
     3001 ttttcctcgg ggatatttaa gcccttgccg tttttcttaa ctttttcttt atttcctaaa
     3061 agaattatat cggcaatgcc ttctttaagt gcaaccgatg ccgccttaag tgttcttatg
     3121 tcttccgatt ccggcaatac tatggtcttt ttattactct tggctctttg tattgttctt
     3181 tctaaaaatg tcattttata tccacctcaa atttaagtat ttaactaata cattatatta
     3241 ccgttaaatt atatattcaa gtcaaagcga acaaaaacct ccgcctatcc gttcattttt
     3301 ttatcagacc tgcggaggtt tctttttttt gtttttatta ccgtattact gtgttatcgc
     3361 ataagtaaga tacagttcct gatacggttt gtaaagccct accttatatt gctccatatc
     3421 cttatactca ttcatcttca tctcaaaatc actggaagca atcagcttga atatgtcatc
     3481 cttcatgtct tcataatcgg ttctgtcgat aagctttatt attacatgtc ctatctcggt
     3541 ctctataaga tccagatctc ctacggaagc cttaagtgcc cacttagata attccgttgt
     3601 tcctacttcc ttcttggtaa atgtgaattc acccggtttt gcggcatcat cggtttcttc
     3661 ctccgactct tcggcttcct gatcattcag gtcatcactg taaagtttcg ccagttcata
     3721 aatatcgcca ccgctctttg ccatttcaaa tatctccgcg gcttttacct tggctgcagc
     3781 tataacatcc tctgcaagag gttgaaactg atcgtcaacg gtacgtttta aaatagtaac
     3841 tacagtaaat ctgtctatct catctctgac ggcatttcct tcggcatcaa ccgaattgta
     3901 atatgagagt gctctttctt cggttaccgc actgatgttt tgttcttcca gataggttgt
     3961 gtattgctgt gctctcaaag tctgcctgat aactttttgc attgccttaa atgatgtgcc
     4021 gtagaatagc ttggcatatt tgtcaagatc atagcccgat tcatccgccg ctctctgaat
     4081 ggcttccaac tgtatggcta aactctcctc caactcttct tcatcaatct taaaacccgt
     4141 ctcttttatt tggtctaaca taatatattg ttcctgtatg gaatccacta caccttcaag
     4201 cagcatctga ccgaaagaac gctgattatc atatgccgtc ttcataaaag ccgatattcc
     4261 ttccggtgtt gacagatcaa agccgagatt tctgatatag tcctgataat agttagcaaa
     4321 gactttatac tgtgtattgg tctggtacat gaactcttct tcggcaattt tatgattgcc
     4381 tattttaata tagttattgg gtatcaaact ggctataacg aaatatatta aacaaaggcc
     4441 gatgataacg gcaagcgtca gaaatccgta ccttattatt gtttcttctt tggatatttc
     4501 cttgctgctc ttcttcttga catcttcttt tttgccttta gcgactgcca tttcctatct
     4561 cctttttctt aaggtaaata aaccgttttc cgtctaccta ttctacaata aacgactgct
     4621 ctttatcaag ccttattttg attcaacagc tttttccagt atttttgtta taaaatccaa
     4681 caattcctca ttctttttat cggtatccac ccttatttgc aaatcttcct gcgctttgac
     4741 agtatatttg atacgctctt tatccagcac atcggtaatc ttgcgaaaat gtatagcatg
     4801 ctctttttca tatatcatga caaaaccttt gtttgtctgt acaatttccg atatcaccaa
     4861 tccgcatgcc atattctttg cgtatgccac atttattata ttaacgacat gaccgggagg
     4921 ctgattatac cggtctgtca actcgtctat tatgtcttcc ttttccgtaa ccgttgcgac
     4981 cgttgctatc ctcttataca tcttaagtct ggataagtca tcggatatat aggacttgtc
     5041 gatatatgca cttatattta aatccacact tgtttcggta actgcacctt taactttttc
     5101 tccccgtaag tttgcaatct cttccgaaag aatcctgaca tacatgtcat agcctacacc
     5161 ctgcatatgc cccgattgat ttgctcctaa aaggtctcct ccccctctta tctccagatc
     5221 cttcatcgca atcttgattc ccgaaccgaa ctcggtatat tcttttatgg tctgtaatct
     5281 cttggaagct tcttcggtca gttctttgcc tcttttgtag gtgatatatg cgtaggcaac
     5341 cctgtccgat ctgcccacac gccctttaat ctgatataac tgtgctaacc ccagtttgtc
     5401 ggcatcttct attatgagag tgttggcatt ggatatgtct ataccggctt ctataatagt
     5461 ggtacacagt aaaacatcat attctcggtt tatgaacttt attatggtat tttcaaactc
     5521 tttttccggc atctgtccgt gtgcaacaca tatcctgact tgatctttta agagtgtttt
     5581 aagatgataa aacttgctgt ccatatccat aacacggtta tacagataaa aaacctgccc
     5641 gttacggtcc ttttcccgta agatt
//
LOCUS       DAOPWC010000044         5632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14328_length_5632_cov_3.075489, whole genome shotgun sequence.
ACCESSION   DAOPWC010000044 DAOPWC010000000
VERSION     DAOPWC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14328_length_5632_cov_3.075489"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKJ65_01720"
     CDS             complement(<1..590)
                     /locus_tag="PKJ65_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40589.1"
                     /translation="MVNKRKTCMTIIAVLIVIVLIALFSVSCKKVQDPLAGFNITTFN
                     GDIVIKRVDGKEPLNMPYRYSMALLAKRLVFRNEIAGINISSVKYEISDTGLRLYNYK
                     GMLVDADSSAVNEVIDSIKYCKGVTTLSGIIADKEDCKIKFYEGCSAYMLVDNLRDYA
                     IIPSTLSEHINKGLSDSEKVFSIMNPKTFGTVQFEI"
     gene            complement(698..2311)
                     /locus_tag="PKJ65_01725"
     CDS             complement(698..2311)
                     /locus_tag="PKJ65_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40590.1"
                     /translation="MNDLITLLKADLINTYKLNGLKDKSNRKKAIGMSLLALFLTAIA
                     FLYIGGAAYLVSDILKSMGLLNILLVFAFALPFLVLFMVNIYKMPSELFSFKDYDMLM
                     SLPVKDTVILAAKVIKLMLSGYVWQFYTSFPIFLVYCIKSDSINAFSVIIYIILSIVL
                     PLLPMSIGSVIGFVVARISAKIKSRNIIMIAGSFALLALIMVFSFSLQNILDEQAITD
                     MVTSIADIQSSFFLFDLYVDAVYGLNILSLLLFILIFTVPFILFIYLFAKSFKKINSS
                     MMETKAHHSSKNVKFKEYSPVMAIFKKELNFYVHCVVYIVNTFFGMVLLLIGCIAVLV
                     VNFDPESVFAEIAQAQGSLSVADIKLLAAATVFGYCIMMSSPTSASISLEGRNLWILK
                     SMPVETKDIFKGKTALGLVVTLPIGIISSVLIYIGLKLSFTQWLILLAFTVSAGFLTA
                     VLGLFANILLPKMEFKSPTEVVKQSASVAVAVFSGMFLVIIPMISGAALIMYNTSLFV
                     LIVASIYAVITLILWRYLITKGVKRFAVL"
     gene            complement(2304..3017)
                     /locus_tag="PKJ65_01730"
     CDS             complement(2304..3017)
                     /locus_tag="PKJ65_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40591.1"
                     /translation="MLRIENLSKTYANKVKAVDDISFHIEPKDMFGFIGHNGAGKTTT
                     LKCVAGILEFEKGEIYINGKSIKEDPVGCKADIAYVPDNPDVYESLTGIQYLNFVADI
                     FKVSKHDRERLTEYYAEQLTFTHALGDIISSYSHGMKQKLVIISALIHSPKLLIFDEP
                     FTGLDPQASFTVKEIMKDICNKGGAVFFSTHVLDVAEKICNKIAIIKNGKIAALGDIQ
                     SVMGDKSLESTFMELAKNE"
     gene            complement(3010..3666)
                     /locus_tag="PKJ65_01735"
     CDS             complement(3010..3666)
                     /locus_tag="PKJ65_01735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ40592.1"
                     /translation="MARINVNPTRMELNNLKRRLITARRGHKLLKDKRDELVKQFMTI
                     VKENKRLREICERMLGEAQNDFLLSRASISDQIIEESLMYPKKTIRLDVQTKNIMGVK
                     VPVITDIDEEESDPNIYPYGILSTTAELDSALYNITEVIPYLIKLAEKEKTVQLMASE
                     IERTRRRVNALEYIMIPQFEETIKYITMKLEENERGNISRLMKVKDMMIEEMRSKKDA
                     "
     gene            complement(3653..5050)
                     /locus_tag="PKJ65_01740"
     CDS             complement(3653..5050)
                     /locus_tag="PKJ65_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ40593.1"
                     /translation="MIKEYRTINEVYGPLMIVKDVENVKYDELGEIELSNGETRKCKV
                     LEIDGTDAIVQLFESSAGINLADSKVRFLGKSLDLPVSSDMLGRVFDGMGNPIDDGPR
                     IIADKRADINGEPMNPTARDYPSEFIQTGISSIDTLNTLVRGQKLPIFSGSGLPHPAL
                     AAQIARQAKVLNVDANFVVVFAAMGITFEEADFFIQDFKKTGAMDRTVMFINLANDPP
                     IERISTPRMALTAAEYLAFEQEMHVLVILTDITYYADALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGRRKGNKGSITLIPILSMPEDDKTHPIPDLTGYITEGQIILSRELN
                     KKSILPPIDVMPSLSRLKDKGIGKGKTREDHSDVMNQLFAAYSRGREVRELAIILGEA
                     ALTETDKLYAKFAEAFENVYINQGFYENRSIEQSLDLGWKLLVKLPRSEMKRVRDANL
                     TKYYDKFKESDDGQD"
     gene            complement(5047..>5632)
                     /locus_tag="PKJ65_01745"
     CDS             complement(5047..>5632)
                     /locus_tag="PKJ65_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ40594.1"
                     /translation="LSEPVTQATMRIVKVFWSLDSSLAYARHFPAINWLTSYSLYASK
                     LDEWSDENIAKNYSTLTKDAMRLLQEEANLQEIVRLVGIDALSNPERLVLEAARSLRE
                     DFLYQDAMHPFDTYCSVNKRYKLLKTIMDYYYRAKEIINDDIDLDKLFGMPVREKISR
                     ARLVEEQNIDDYFCTIDDEMEKQFKDFSDKEALS"
BASE COUNT         1635 a         1257 c          986 g         1754 t
ORIGIN      
        1 attatctcaa actgaaccgt accaaatgtc ttcggattca ttattgaaaa aaccttttca
       61 gagtcggata atcccttatt aatatgctca ctcaaggtag agggaataat agcataatcc
      121 cgaagattat ccacaagcat ataggcggag cagccttcat aaaattttat cttacaatct
      181 tctttatcgg caattatccc gcttaatgtt gttactcctt tacaatactt tattgaatct
      241 ataacttcat taactgcgct gctgtccgca tcaaccaaca tgcctttata gttatataat
      301 cttaaacctg tatcacttat ctcatatttt acggaagaaa tgttgatacc ggcgatttca
      361 tttctgaata ccagtctttt agccaataaa gccatggaat atctgtacgg catattcaac
      421 ggctctttac cgtccactct tttaataact atatcgccgt taaaggtggt tatattaaaa
      481 ccggccaaag ggtcttggac ttttttgcaa gataccgaaa ataacgctat caagacaatc
      541 actatcaaaa cagctattat agtcatacag gtttttctct tgtttaccat gccgttaacc
      601 tccggattta taacataaaa atcggttaat caaacttctc tttagacaca atggtactat
      661 gtataatatt ttatcataaa aagatatgta tttgatacta aagtacggca aacctcttaa
      721 cacccttggt tatgagatag cgccatagaa ttaaggttat gacggcatat attgaagcta
      781 ctatcaggac aaacaaagat gtattataca ttatcagtgc ggcacccgaa atcatgggga
      841 ttattactaa aaacatcccg gaaaaaaccg ccacagccac cgatgccgac tgtttaacaa
      901 cttccgtcgg ggatttgaat tccattttcg gaagaagaat gtttgcaaac aaacctaaaa
      961 ccgctgtaag aaatcccgcc gatacggtaa atgcaaggag aataagccac tgtgtaaaag
     1021 acagttttaa accgatatag atgagcaccg acgatattat ccctataggc agagtaacaa
     1081 caagtcctaa agctgttttt cctttaaaaa tatcttttgt ttcaacgggc atggacttca
     1141 atatccaaag attcctgcct tccaaagata tggaagcgga ggtcggactg ctcatcataa
     1201 tacaataacc gaataccgtc gctgccgcca gaagctttat atctgcgaca gataaactgc
     1261 cttgtgcctg tgcaatttcc gcaaagaccg attccggatc aaaattcaca accaaaaccg
     1321 ctatacaacc tattaataac agtaccatac cgaagaaagt atttactata tacacgacac
     1381 agtgaacata gaagttgagt tcctttttaa atattgccat taccggagaa tactctttga
     1441 acttgacatt ctttgatgag tgatgtgcct ttgtttccat catgcttgag tttattttct
     1501 taaaagattt tgcgaacaga taaataaaca gtatgaacgg aacggtgaat atgagaataa
     1561 acagaagcag cgataatata ttcagaccgt aaaccgcatc aacatacaaa tcaaacagga
     1621 aaaaggatga ctgtatgtcc gcaatggaag taaccatatc ggtaatagcc tgttcatcca
     1681 atatattctg taaagaaaaa gaaaaaacca ttataagggc aagtaatgca aaagagcccg
     1741 caatcataat tatattcctg cttttaatct ttgcggatat tcttgccaca acaaacccta
     1801 tcacggaacc tatactcata ggaagaagag gcaacacaat agaaagtatg atgtatatga
     1861 ttacggaaaa agcatttatg ctgtcggatt taatgcagta aaccagaaaa atcggaaaac
     1921 ttgtataaaa ctgccacaca taaccgctta acataagttt tattactttt gcggcaagta
     1981 tgacagtatc ttttaccggt aacgacataa gcatatcgta atccttgaaa gaaaacagtt
     2041 ccgacggcat cttatatata ttgaccataa acaaaactaa aaaaggtaac gcaaacgcaa
     2101 atacaagcag tatgttcaga agtcccatac tcttcagtat atcggagaca agataagcgg
     2161 ctcctcctat atatagaaac gctatagctg tcagaaaaag agcaagcagg gacataccta
     2221 ttgctttttt tctgtttgac ttatctttaa gtccgttcag tttgtatgtg tttataaggt
     2281 cggctttcag aagcgtaatt aaatcattca ttttttgcaa gctccatgaa agtgctttcc
     2341 aaagacttat cccccattac actttgtata tcccctaatg ccgctatttt tccgttcttg
     2401 ataatggcta ttttgttgca tattttttcc gccacatcca atacatgcgt ggaaaagaaa
     2461 accgctccgc ccttattgca aatatctttc attatttctt ttacggtaaa ggaggcttgc
     2521 gggtccagac cggtaaaagg ttcatcgaat ataagcagtt tgggactgtg tatgagtgcg
     2581 gatattataa ccagtttctg tttcatgccg tgtgaatatg aagatattat atcacccaaa
     2641 gcatgggtaa aggtcagttg ctcggcataa tattcggtaa gtctctcgcg gtcatgcttt
     2701 gaaactttaa aaatgtccgc aacgaaattc agatattgta ttccggtcaa tgattcatat
     2761 acatcgggat tatccggtac atatgcaata tccgctttgc atccgacagg atcttctttt
     2821 atgctttttc cgttgatata aatttcccct ttttcaaact ccagtatacc ggcaacacat
     2881 ttcagtgttg tggtcttacc cgctccgtta tgccctataa aaccgaacat atctttaggt
     2941 tcaatatgaa aggagatatc gtcaaccgct tttactttat ttgcataggt tttggaaaga
     3001 ttttcaattc taagcatctt tcttacttct catctcttct atcatcatat ccttgacctt
     3061 catcagcctg gatatgttgc cgcgttcgtt ttcctctaac ttcatagtga tatatttaat
     3121 agtttcctca aactggggaa tcattatata ttccaacgcg ttgactcttc ttcttgtcct
     3181 ctcaatctcc gatgccataa gctgtacggt tttttccttt tccgccagtt ttatcaaata
     3241 gggtatgacc tcggtgatgt tgtataaggc gctgtccagt tcggcggttg tactcaatat
     3301 accgtacgga tatatattgg gatcgctttc ttcttcatcg atatcggtaa ttacgggaac
     3361 cttaaccccc attatattct tagtctgcac atccagtctt atggtctttt tgggatacat
     3421 aaggctttct tctataatct gatcggaaat ggatgctctg gacagtaaaa agtcattctg
     3481 cgcttctccc agcatacgct cgcagatttc ccgaagcctt ttattctcct ttacaatagt
     3541 cataaactgc ttaacaagct catcccgctt atcttttaag agcttatgac ctcttcttgc
     3601 ggttataagt ctccttttga gattgttaag ttccatcctt gtaggattta cattaatcct
     3661 ggccatcatc cgattcctta aatttatcgt aatacttggt cagattggca tccctgaccc
     3721 ttttcatttc acttctgggc agtttcacca gtaatttcca acccaaatcc agcgattgct
     3781 ctatggacct gttctcatag aatccctggt ttatatatac attttcaaaa gcttcagcaa
     3841 atttggcata caacttgtcc gtttcggtca aagccgcttc acctaatata attgccagct
     3901 ctcttacttc cctacctctg gaatatgctg cgaaaagctg attcataaca tccgaatggt
     3961 cctctctggt cttgcccttt cctatccctt tatctttcag acgggaaagt gaaggcatta
     4021 catctatagg aggtaatatg gattttttat tcagctctct ggataatata atctggcctt
     4081 ccgttatata tccggtaagg tcgggaatgg gatgtgtttt atcatcttcc ggcatggaca
     4141 gtataggtat cagagttata ctgcctttgt tgccttttct tcttcccgcc cgctcataca
     4201 tggtggcaag gtcggtatag agatatccgg gataacctct tcttccggga acttctttct
     4261 tagcggccga tacttctctt aacgcatcgg catagtatgt aatatcggtc agaataacca
     4321 gaacatgcat ttcctgttca aatgccagat actccgcagc cgtcagagcc attctcggtg
     4381 tggatatcct ttcaatcgga ggatcgttgg caagattaat aaacataacg gttctgtcca
     4441 tggctccggt cttcttgaag tcctgtatga agaaatccgc ctcttcgaat gttataccca
     4501 ttgccgcaaa aaccacaacg aaattggcat ccacattcag gacttttgcc tgtcttgcaa
     4561 tctgagccgc aagtgcggga tgcggtaatc cggaaccgga gaatatcggc aacttctgtc
     4621 ctcttaccaa agtattgaga gtatctattg aagagatacc ggtctgaata aactccgaag
     4681 gataatccct tgccgtagga ttcatgggtt caccgtttat atccgctctt ttgtccgcaa
     4741 taatccgggg accgtcatct atggggtttc ccattccgtc aaacaccctg cccagcatat
     4801 cgcttgatac cggcaaatcc agactcttgc ccaaaaacct tactttactg tcagcaaggt
     4861 ttattccggc ggaactctca aacaactgaa ctatggcatc ggttccgtct atttccagca
     4921 ctttacactt tctggtttca ccgttggaaa gctctatctc gcccaattcg tcatacttga
     4981 cgttttccac atcctttact atcatcaaag ggccgtatac ttcattaata gtcctgtatt
     5041 ctttaatcat gataaagcct ccttatcgga aaaatctttg aactgctttt ccatttcatc
     5101 atctatagta caaaaatagt catcaatatt ctgttcttca acaagccttg cacgtgaaat
     5161 cttctcacgt accggcatac cgaataattt atccagatca atatcatcat ttattatctc
     5221 tttggctctg taataataat ccataatggt cttcagaagt ttataccgct tatttacact
     5281 gcaataggta tcaaacgggt gcatggcgtc ctgataaaga aaatcctctc ttaaagaccg
     5341 tgccgcttcc agaaccagtc gctcgggatt ggaaagcgcg tctataccta caagtctcac
     5401 tatttcctgc agattcgctt cttcctgcaa cagcctcatg gcatctttgg taagtgtcga
     5461 ataattcttt gctatgtttt catccgacca ttcatccaat ttggatgcat agagagagta
     5521 actggtcagc cagtttattg ccgggaagtg tctggcataa gccaaagagg aatccagact
     5581 ccagaacacc tttacaattc tcatcgtggc ctgtgtaacc ggttcggaaa gg
//
LOCUS       DAOPWC010000045         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14468_length_5591_cov_3.549855, whole genome shotgun sequence.
ACCESSION   DAOPWC010000045 DAOPWC010000000
VERSION     DAOPWC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14468_length_5591_cov_3.549855"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      259..358
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            413..895
                     /gene="purE"
                     /locus_tag="PKJ65_01750"
     CDS             413..895
                     /gene="purE"
                     /locus_tag="PKJ65_01750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNZ40595.1"
                     /translation="MKVLVLMGSDSDCKTVEPGLKFLEEYNIEYSVRVCSAHRTPDMA
                     KQLAVNAKSEGFKVIIAAAGKAAHLAGVIAAHTVLPVIGIPVKTSDLGGMDSLLSTVQ
                     MPAGVPVATVAIDGGKNAAILAAEILALNNEELSNKLQQFKKELEQKVMQADREINNK
                     "
     gene            897..2264
                     /gene="purF"
                     /locus_tag="PKJ65_01755"
     CDS             897..2264
                     /gene="purF"
                     /locus_tag="PKJ65_01755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ40596.1"
                     /translation="MSGVFGVYSKDKSVNIAEKIYYGLYALQHRGQQSAGIAVRDKDL
                     IEWHKDKGLVPEVFKGPDLDKLPGDAGIGHVRYSSSGDVSKGNIEPLVLKYISGYMAI
                     AKNGNIINANHLRGKLEADGCMFHTTNDTEVLAALLTKFRISSEHIEDSVEQMVKYIQ
                     GSYSIVMITPRFLIGLRDPLGIMPLCIGKINGATYVISSESAAIDAVDGEFIRDVEPG
                     ELVLIDSDGIQSRRLFDCKKSSLCIFEHVYFARPDSIVDGASTHDARVNAGRILAKEH
                     PVQADVVIGIPDGGTDMAYGFAQESNIVAPAGLLRNKYVGRAFIAPNQNAREIAVKIK
                     FNPVKTEVKGKRVVLIDDSIVRGTTTKTLVRMLREAGAKEVHLRIGSPPYKFPCYFGV
                     DTSSRGQLLASKNDKEAICKWIGADSLEYISYEGIIKAPVNSKVDFCTSCFTGVYPVD
                     VPKEF"
     gene            2278..3330
                     /gene="purM"
                     /locus_tag="PKJ65_01760"
     CDS             2278..3330
                     /gene="purM"
                     /locus_tag="PKJ65_01760"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ40597.1"
                     /translation="MKDAYKEAGVDVQAGYESVALIKKHIQRTVTEGVLGGIGSFSGL
                     FQINLNKYPTPVLVSGTDGVGTKLKLAHSMKVHDTIGIDAVAMCVNDVLCQGADPLFF
                     LDYIACGKNYPDIIEKIVKGVAEGCIQAECALVGGETAEMPGFYKQDEYDIAGFCVGA
                     VNKPDIIDGSLIEEGDVLLGLASTGVHSNGFSLVRKIFGEDAKSLSKVYPGYEATIGE
                     VLLTPTKIYVKDIKKIRAVAKIKGMSHITGGGFVENIPRMFKDNNLTPVINKGSWAVL
                     PVFDLIMKQGNITEDNMYRTFNMGIGTVIAVSKEDAKKIQKQFKNEIYEIGYVALAGD
                     VKKLKDIEENIYIKER"
     gene            3332..4876
                     /gene="purH"
                     /locus_tag="PKJ65_01765"
     CDS             3332..4876
                     /gene="purH"
                     /locus_tag="PKJ65_01765"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNZ40598.1"
                     /translation="MIRRALISVSDKSGIAEFAKRLSDMGVEIISTGGTSKVLSENGI
                     KVIPIESITGFKECLDGRVKTLHPNIHAGLLAIRDKKDHMDTLESLDITPIDMLVINL
                     YPFKKTVQKSNVTKEEAIENIDIGGPAMLRSGAKNHATVTVITDPSDYESVLNELEQN
                     GKVSYETNFRLAIKVFEHTANYDAMIADHFNKLTGDYSLKNTFTVTYEKLQEMRYGEN
                     PHQKAAFYKEPAITKGTICDAVQLHGKELSYNNINDADGALSMLKEFPDKPACVAVKH
                     ANPCGVGVGKNVFEAYKKAYDSDPVSIFGGIVAFNRTVDVVTAEKLNEIFLEIVIAPD
                     YKDEALTLLMKKKNLRILRLDNITYNNENEYIMKKVSGGMLVQSKDNCVVNPEDIKVV
                     TVKQPTEQQLKDAVFGMTVCKHVKSNAIVLVKDLASIGIGPGQMSRIGAAKIAISLAG
                     ERAKGSVMASDAFFPFDDCVIAAKEAGVECIIQPGGSLADKDVIAKADQFGICMIFTG
                     IRHFLH"
     gene            4889..>5591
                     /gene="purD"
                     /locus_tag="PKJ65_01770"
     CDS             4889..>5591
                     /gene="purD"
                     /locus_tag="PKJ65_01770"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNZ40599.1"
                     /translation="MKVLVIGGGGREHAVVKKLSESPKIKKIYCAPGNAGMGKIAQCV
                     NIKATDIDGILDFLKDNPVDLVVVTPDDPLALGLVDKLNERNIRAFGPVAKAAEIESS
                     KAFAKNLMKKYGIPTGAFEVFDNPEKALQYVKNRQYPLVIKASGLALGKGVIIVENYR
                     QAEETINQMMINDMFGSSGHTVIIEEFLQGPEVTVLAFTDGKTIKTMPSCQDHKRVFD
                     NDKGPNTGGMGAFSPS"
BASE COUNT         1856 a          976 c         1400 g         1359 t
ORIGIN      
        1 gaaatattaa attatgtaaa gtaggaaatt ttaccgaaag gagaaaaagt cagcaatatt
       61 tgtagttttt tctaacaatg agaaaaagat atgtaaaatt tggatttttc tttaaaatga
      121 gaaaaaacta ttgagaggta ttgttaatat cggacatttt cttaacgata tttccgtttt
      181 ttatcaatag aagaaacaaa taatgtatat gatttgaaac agccgttcgt gttgacacat
      241 aaagggaggg agtgataaaa tattttcttg aagagagctc gtatatgttg caaaaaacgg
      301 tctgcaacgt ttctaccggg acaccttaga cccgactatg agtggcttat tccattgcgt
      361 tgttttttat ggtgcagtca ctacgagact gcatttttat tgggaggatt aaatgaaagt
      421 attggtactg atgggaagcg acagcgactg taaaacggta gaacccggtc ttaaatttct
      481 ggaggaatat aatattgaat atagtgtaag ggtatgttcc gcgcaccgca cacccgatat
      541 ggccaagcaa ctggccgtaa atgccaaaag tgaaggtttt aaagtcataa tagcggctgc
      601 cggaaaagcg gcccatttag cgggagtcat agccgcacat accgtgttgc cggttatagg
      661 gattcccgtt aagacaagcg atttaggagg catggattca ctcctttcaa cggtgcagat
      721 gcctgcggga gtaccggttg ccacggtggc gattgacggc ggaaagaatg cagcaatatt
      781 ggcagccgag atactggccc tgaacaatga agaattaagc aataaattgc aacagttcaa
      841 aaaagaattg gaacaaaagg taatgcaagc ggacagggag ataaacaaca agtaatatga
      901 gcggagtttt cggagtatat tcaaaagata aatcggttaa catagcggag aaaatatatt
      961 acggactgta tgccctccaa cacagaggac agcagagtgc cggtatagcg gtcagagata
     1021 aagatttaat agagtggcat aaagataaag gtcttgttcc cgaggttttt aaaggtccgg
     1081 atctggataa actgccgggg gatgccggaa taggtcatgt gcggtattcc tcgtccggcg
     1141 atgtatccaa aggaaatatc gagccgttgg tactgaagta tataagcgga tatatggcca
     1201 tagcaaagaa cggcaatatt attaatgcaa atcacttaag aggaaagctg gaagccgacg
     1261 gatgtatgtt ccataccacc aatgataccg aagtactggc ggcacttctg accaagttcc
     1321 gcataagcag cgaacatata gaggattcgg tggagcagat ggtgaaatat attcagggtt
     1381 cctactccat tgtcatgatt acccccagat tccttatagg tcttcgggat cctctgggca
     1441 taatgcctct ttgtataggc aagattaacg gtgcaacata tgtcatttcc agcgaatcgg
     1501 ccgctataga tgcggttgac ggggaattta taagagatgt ggagccggga gagctggtac
     1561 tgatagattc cgacggtatt cagtcaagaa ggttgtttga ctgcaaaaag agcagtttat
     1621 gtatattcga gcatgtatat tttgctcggc cggacagcat tgttgacggg gcatcgacac
     1681 atgacgcaag ggtaaatgcc ggaaggattc tggcaaaaga acatcccgta caggcggatg
     1741 tggtaatagg aattcccgac ggcggaaccg atatggcata cgggtttgca caggaaagta
     1801 atatagttgc acctgcaggt ctgttaagga ataaatatgt gggcagggcg tttatagcac
     1861 ccaatcagaa tgccagagaa atagctgtaa agataaaatt caacccggtc aagaccgagg
     1921 tcaaaggtaa aagagtggta ctcattgatg attccatagt cagaggaacc acaaccaaga
     1981 cacttgtcag aatgctgagg gaagcgggag ctaaagaagt tcacttaagg ataggctctc
     2041 ctccatacaa gttcccttgc tatttcggag tggacacttc cagcagagga cagttgctgg
     2101 ccagcaagaa tgataaagag gcaatatgca aatggatagg tgccgacagc ctggagtaca
     2161 tcagttatga aggaattatc aaagcacctg tcaattccaa agtggatttt tgtacttcct
     2221 gttttacagg agtctatccc gtggatgtac ctaaagagtt ttaggaggat aagtgctgtg
     2281 aaagatgcat acaaagaagc gggagtggat gttcaagcag gttacgagtc tgtggcttta
     2341 attaaaaagc atattcaaag aacggtaacc gaaggggtgc tgggcggaat aggaagtttt
     2401 tccggattat tccaaataaa cttgaataaa tatcctaccc cggtattggt ttcgggaacc
     2461 gacggagtgg gtaccaaact gaaacttgct cattccatga aagttcatga cacgataggg
     2521 atagatgcgg tggcaatgtg cgtcaacgat gtactgtgtc agggagccga cccgctattc
     2581 tttttagatt atattgcatg cggcaagaat tatcccgata taattgaaaa gatagtaaaa
     2641 ggtgttgccg aaggatgtat acaggcagaa tgtgccctgg tgggaggaga aacggccgag
     2701 atgcccggct tctataaaca agacgaatac gatatagcgg gattttgtgt cggagcggtc
     2761 aacaagcctg atataattga cggatcgttg atagaagaag gcgatgtgct tttgggattg
     2821 gcatcaacgg gagtgcacag caacggtttt tccttagtac gtaagatatt cggagaggat
     2881 gcaaaaagct taagcaaagt atatcccgga tatgaagcaa ccataggtga ggtgcttctt
     2941 acacctacaa agatatatgt aaaagatatt aaaaaaataa gggccgttgc taaaataaag
     3001 ggaatgagcc atattaccgg aggaggattt gtggaaaaca taccgaggat gtttaaagac
     3061 aacaatttaa ctcccgtaat taacaagggc agctgggcgg tgttacccgt atttgacctc
     3121 ataatgaagc aagggaatat aaccgaagat aatatgtacc gtactttcaa tatgggtata
     3181 ggaacggtaa tagcggtaag caaagaagat gcgaagaaaa tacaaaaaca gtttaagaac
     3241 gaaatatacg agataggata tgtggcttta gccggggatg tcaaaaagtt aaaagatatt
     3301 gaggaaaata tatatataaa ggaaagataa tatgattaga agagcattga taagtgtaag
     3361 cgacaaaagc ggaattgcgg aatttgcaaa gagattgagt gatatgggtg tggaaataat
     3421 ttccaccggc ggcacttcaa aagtacttag cgaaaacgga ataaaggtta ttcccattga
     3481 aagcataacc ggctttaagg aatgtctgga cggaagagta aagacgctgc accccaatat
     3541 acatgcgggt ctgttagcta taagagataa aaaagaccat atggatacat tggagagtct
     3601 ggatatcacc cctatagata tgttggttat taatctctat cctttcaaaa agaccgtaca
     3661 gaaaagcaat gttaccaaag aagaggctat tgagaatatt gatataggcg gaccggcaat
     3721 gttgagaagc ggtgccaaga atcatgcaac ggtcacggtt attactgatc cttctgatta
     3781 tgaaagtgta ttaaatgaat tggaacaaaa cggcaaagta tcttatgaga ccaattttag
     3841 acttgcaata aaggtatttg aacacaccgc taattacgat gcaatgatag ccgaccattt
     3901 caacaagctt acaggagatt acagcttaaa gaatactttc accgttactt atgagaagct
     3961 tcaggaaatg agatacggag agaatcctca ccagaaagcc gccttctata aagaacccgc
     4021 aataacaaag ggaaccatct gcgatgcggt gcaactgcac ggcaaagaac tatcatataa
     4081 caacataaat gatgccgacg gtgcattgtc catgctcaaa gagtttcccg acaaacctgc
     4141 atgtgtggcg gtaaagcatg ctaacccctg cggtgtggga gtgggcaaaa atgtatttga
     4201 agcatacaaa aaagcgtatg attccgatcc ggtttccata ttcggaggaa tagtcgcatt
     4261 caacagaacg gtggatgtag taactgccga aaagctcaat gagatatttt tggaaatagt
     4321 tattgcaccc gattacaaag atgaagctct tacattatta atgaaaaaga agaatttaag
     4381 aattcttaga ttggataaca taacatataa caatgaaaac gaatatatta tgaagaaagt
     4441 aagcggggga atgcttgtac aaagcaaaga taattgcgtt gtaaacccgg aggatattaa
     4501 agtcgtgacc gtaaaacagc ctaccgaaca gcagttgaaa gatgccgttt tcggaatgac
     4561 tgtatgcaag catgtcaaat ccaatgcgat agtacttgta aaagacttag cttcgatagg
     4621 aataggtccc ggacagatga gcaggatagg agcggccaag atagccatat ccttggcggg
     4681 agaaagggct aaaggcagcg tcatggcatc ggatgcgttt ttcccgtttg acgactgtgt
     4741 catagcggcc aaagaagcgg gcgtggaatg cataatacaa cccggaggat cgttagccga
     4801 taaggacgta attgctaaag cggaccaatt tggtatctgt atgatattca cgggaataag
     4861 gcacttttta cactgagaaa ggtaaaagat gaaggtactg gtaatagggg gaggcggcag
     4921 agaacatgcc gtagtaaaaa aactttccga gagtccgaaa attaaaaaga tttattgtgc
     4981 tccgggtaat gccggaatgg gaaaaatagc gcaatgcgtc aacataaagg ctaccgatat
     5041 agacggtata ctggattttc ttaaagacaa ccctgtggac ctggttgtgg tcactcccga
     5101 tgacccgtta gctttgggac ttgtggataa actgaacgaa agaaatatac gtgcgttcgg
     5161 accggtagct aaagcggctg aaatagaaag cagtaaagct tttgccaaaa accttatgaa
     5221 gaaatacggt atacctacgg gtgcttttga agttttcgat aaccccgaaa aagctttgca
     5281 atatgttaaa aacaggcaat accctctggt tataaaagcg tcgggactgg cgctgggtaa
     5341 aggtgtgata attgttgaaa attatcggca ggcagaggaa accataaacc aaatgatgat
     5401 aaacgatatg ttcggctcat ccggccacac cgttataatc gaagagtttt tacagggacc
     5461 ggaagttacc gtactggcat tcaccgacgg taaaaccata aagaccatgc cgtcgtgtca
     5521 ggatcataaa agagtatttg ataacgacaa aggtcccaat accggcggca tgggcgcttt
     5581 cagtcccagc a
//
LOCUS       DAOPWC010000046         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14538_length_5570_cov_3.482684, whole genome shotgun sequence.
ACCESSION   DAOPWC010000046 DAOPWC010000000
VERSION     DAOPWC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14538_length_5570_cov_3.482684"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="prfA"
                     /locus_tag="PKJ65_01775"
     CDS             complement(<1..842)
                     /gene="prfA"
                     /locus_tag="PKJ65_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNZ40600.1"
                     /translation="MLGKLKEIEIKYEDISRQLTDPAVISDQEQYVALMKELNGISEI
                     VKVYREYVQTAKEIDDIYIMLKENSDDADFKQLLQEELAQLEEKKPALEQQLKILLLP
                     VDPNDTKNVIIEIRGGAGGDEAALFAADLLRMYTMYAEAKKWKTEILDLNEIGIGGIK
                     EVVFSITGQGAYSRFKYESGVHRVQRVPETESQGRIHTSTVTVAVLPEAEEVDVEINM
                     NDVQIDTYRSSGAGGQHVNKTDSAIRLTHIPTGIVVACQDERSQHKNRDKAFKILRSK
                     LYEIA"
     gene            complement(990..2108)
                     /gene="wecB"
                     /locus_tag="PKJ65_01780"
     CDS             complement(990..2108)
                     /gene="wecB"
                     /locus_tag="PKJ65_01780"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNZ40601.1"
                     /translation="MIKIMSVFGTRPDTIKMAPLVNLLDKEKEIDHIVCVSGQHREMV
                     NEILDIFKITPKYDLDIMRERQTLEHVTSSILMKLSDILSKEQPDMILVHGDTNTCLS
                     ASLAAFYKKIPIGHVEAGLRTDDIYSPYPEEFVRRTVDSISSLYFCPTINNADNLIKE
                     NISKDRIYITGNTVIDTLQTTVKKDYTFKEPLLNSIDYNNKRIIPITAHRRENIGVNL
                     ENICNAVKTLASKYPDAHFIYTVHPNPAVKNTANRILEGIDNVSLLPPLIFTDLHNLI
                     ARAYFVMTDSGGLQEEAPSLGVPVLVLRRETERPEAVQAGTVKLTGVEYKNIVRDGRM
                     LFENKSVYNKMSNAVNPYGDGKASRRIVDAIKEYFKNL"
     gene            complement(2101..3210)
                     /locus_tag="PKJ65_01785"
     CDS             complement(2101..3210)
                     /locus_tag="PKJ65_01785"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ40602.1"
                     /translation="MSYIYTFLASFIIALVFCPLVIKFAKAKKILKYPRRNRDVHTEP
                     VPLLGGIAIIISFGLSVFGNYIFNPNFIVTNEVIGLAVGIVIISVMGILDDIYDLKPV
                     IKVVFQVTAAVATVAISGSKIAYFTNLGTNNNLVHLPDIISFIATVAWIFMLTNAFNI
                     IDGLDGLTAGTTVICSLTLFFVTFVRPDAGLVGEYIPILLIALSGAAAGFLPFNFNPA
                     KLYLGETGAAFTGFCIAVLSVQGTMKAHAAIAVFIPLVAMGLPVLDISLAYFRRIIMK
                     KPIAEGDRDHIHHRLMKMGLSQRKTVLILYLIDIVFGITAFLLSGQQYSRFWLYFAII
                     IILMGLSVFFLYKPLLRQAAKNGNTEVMEEEQSND"
     gene            complement(3212..3658)
                     /locus_tag="PKJ65_01790"
     CDS             complement(3212..3658)
                     /locus_tag="PKJ65_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNZ40603.1"
                     /translation="MERPSWDEYFMQVVHLVKTRSTCLRRAVGAVIVKDKRILATGYN
                     GAPVGCKHCEETGCLRQKMNVPSGEKHELCRAIHAEQNAVVQAAKAGISIDGSTIYVS
                     VQPCVICAKLLINAGIKRIVFEGDYPDALSKELLEEAGIELVKYGG"
     gene            complement(3658..4746)
                     /gene="upp"
                     /locus_tag="PKJ65_01795"
     CDS             complement(3658..4746)
                     /gene="upp"
                     /locus_tag="PKJ65_01795"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ40604.1"
                     /translation="MLAIGCDHAGFTLKSEILPYLEQKNIPYRDFGSYSEDSCHFPFY
                     ASLVASSVQKNECERGILICGTGIGMSIAANKFKGIRAALCMDEYQASMAKRHNNANI
                     LVLAGRITPKDMAHKIIDIWLNETYLEGKYELRNGLIRRIEEGGSMSGTNYMDMAFVF
                     DHPLIQHKISIMRDKNTNTKEFRELVTEVSTLMGYEVTRELPLEQVEVETPIAKTTVK
                     VLSGKSVGFVPILRAGLGMVDGLLTLIPTAKIGHIGLYRDHETLQPVEYYCKLPEDAH
                     ERMIILLDPMLATGGSASAAIGFLKQRGVKNIKLMSIIAAPEGIKKVHDDHPDVNIYC
                     ACVDKALNDDAYIVPGLGDAGDRLFGTK"
     gene            complement(4868..>5570)
                     /locus_tag="PKJ65_01800"
     CDS             complement(4868..>5570)
                     /locus_tag="PKJ65_01800"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ40605.1"
                     /translation="YNKGDELIFDTLNGTSLSVYLKKKGYSPALLKKLLYSIKDMMRI
                     TGSYLIDAEYVVPDTRYIYVFKDETFKYILDPFNKYDLNNGLKQLVSEITEGNYTERS
                     IKEALSSEHFDINGIIKDSHDGNIDVQEKVCEEPKKAVFKNIVDTDDCIIIADKDITV
                     GRLKLICDYRLPGKLASLRHARMRYSHDTVYVRDLGSKYGTYLNGDRIKPESFVKVKR
                     GDIVSFGEDEYILIS"
BASE COUNT         1503 a         1290 c          977 g         1800 t
ORIGIN      
        1 gcaatctcat acagcttact tctcagtatc ttgaaagcct tatccctgtt cttatgctga
       61 cttcgttcgt cctgacaggc cactacaata ccggtaggga tatgggtcag tcttattgcc
      121 gaatcggtct tatttacatg ctgccctccc gctcctgacg atctgtatgt atctatctga
      181 acatcattca tgtttatctc cacatccact tcttccgctt ccggtaatac cgctacggta
      241 acggtggagg tgtgtattct gccctgcgac tcggtttcgg ggactctttg taccctgtgc
      301 actccgcttt catatttgaa tctgctgtat gcaccctgtc ccgttatgga aaataccact
      361 tccttaattc ctcctatacc tatctcgtta aggtccaata tctcggtctt ccatttttta
      421 gcttcggcat acatggtata cattcttaac aaatcggcag caaaaagtgc ggcttcatct
      481 ccgccggcac cgcctcgtat ctctattatt acgttttttg tatcattggg atcgacggga
      541 agcaacagta tcttaagctg ctgttccaag gcaggcttct tttcttccag ttgagccagt
      601 tcctcttgca gtaattgctt aaaatcggca tcatcggagt tttctttaag cataatatat
      661 atatcgtcaa tttcttttgc agtttggaca tactccctgt aaaccttaac tatttccgaa
      721 atcccgttaa gttccttcat taatgccaca tactgctcct ggtcggatat tacagccggg
      781 tcggtcagtt gtctgcttat atcttcatat ttaatctcta tttcttttaa ttttcccagc
      841 atttccacct cttaatattc acttatatta tacattaatt gcaattaaat aaaaaaacat
      901 cataaaaaca aagcaactca taaattatac attaatatcc gtaaatcatt acgaactgaa
      961 atgcaacaac gttgcatctt gttgcttagc tacaaatttt taaaatactc ttttattgcg
     1021 tccactattc ttcgggaagc cttaccgtca ccgtacggat taacggcatt actcatcttg
     1081 ttgtacaccg atttattttc aaacagcatt ctgccgtccc gtactatatt tttatattct
     1141 acaccggtca gttttaccgt tcccgcctgt accgcctcgg ggcgttcggt ttctcttctc
     1201 agcacaagca cgggtactcc caatgaaggt gcttcttcct gcaatccgcc cgaatcggtc
     1261 ataacgaaat atgctcttgc gataaggtta tgaagatcgg tgaatataag gggtggcagt
     1321 aacgatacat tatctatacc ttccagtatc ctgtttgccg tattctttac tgcgggattg
     1381 ggatgcaccg tgtatataaa gtgggcatcg ggatattttg atgcaagagt ttttacggca
     1441 ttacatatgt tttctaagtt tactcctata ttttcccttc tgtgggccgt tatcggtatt
     1501 attcttttgt tattataatc tatactgtta agtaacggtt ctttgaatgt atagtctttt
     1561 tttaccgttg tctgcaaagt gtctataacc gtatttccgg taatatatat tctgtctttg
     1621 cttatattct ctttgataag attgtccgca ttgtttatag taggacagaa atacagtgag
     1681 gatatggaat ccaccgttct tctgacaaat tcctcgggat acggcgaata tatgtcatcg
     1741 gttctcaatc ccgcttccac atgtccaatg ggtatttttt tataaaaagc ggcaagcgat
     1801 gcggaaagac aggtgttggt gtctccgtga accaatatca tatccggttg ctcctttgac
     1861 aggatatcgg acaatttcat cagtatcgac gaggtgacat gctccagcgt ttgtctctct
     1921 ctcattatat ccaggtcata tttcggtgtt attttaaata tatccagtat ctcattgacc
     1981 atttctctat gctgccccga cacgcatact atatgatcta tttctttctc tttgtccaga
     2041 agattcacta aaggagccat ttttattgta tccggtctgg tgccgaatac cgacattatt
     2101 ttaatcattg ctttgttcct cctccatgac ttccgtattg ccgtttttag cagcctgcct
     2161 gagtaacggc ttgtatagga aaaataccga taaacccatc agaatgatta tgatggcgaa
     2221 atataaccag aatctgctgt actgctggcc ggaaagtaaa aaagccgtta ttccgaacac
     2281 tatatctatc aaatacagaa ttagtacggt ttttcgctgt gataatccca ttttcataag
     2341 tctgtgatgt atatgatccc tgtctccttc cgcaatgggt tttttcatga ttattcttct
     2401 aaaatatgcc aaggatatat ccaataccgg caatcccatg gcaacaagag gaataaagac
     2461 cgctatggcg gcatgagcct tcattgtacc ctgaacggat aaaaccgcaa tacaaaaacc
     2521 ggtaaaggcc gctcctgttt ctcctaaata cagttttgcc ggattgaaat tgaagggtaa
     2581 aaagcccgca gcggcaccgc ttaaggctat aaggagaatg ggaatatatt cacctaccaa
     2641 ccccgcatcc ggtcgcacaa aggtaacaaa gaaaagagtg agactgcata ttacggtggt
     2701 tcccgcagta agaccgtcca atccgtctat aatattgaaa gcgttggtga gcatgaatat
     2761 ccatgcaacg gtagctataa aggatattat atccggcaga tgaacaaggt tattattggt
     2821 ccccagattg gtgaaatatg caatcttgga tcccgatatg gcaacggtag caactgccgc
     2881 tgtaacctgg aataccactt ttataacagg ctttaagtca tatatatcat ccaatatacc
     2941 cataaccgat atgattacta taccgaccgc caaacctatt acctcgttgg taacaataaa
     3001 attgggattg aaaatgtaat tcccgaaaac cgagaggccg aaagaaatta ttatggcaat
     3061 accgccgaga agcggtaccg gctcggtatg aacatccctg tttcttctgg gatattttaa
     3121 tattttctta gctttggcaa actttatcac taaaggacag aaaaccaatg caataatgaa
     3181 tgaagctaag aaggtgtata tataactcaa attaacctcc gtactttacc agctcaatgc
     3241 ctgcttcctc taaaagctcc tttgacaatg catcgggata atccccttca aatactattc
     3301 tctttatacc ggcatttata agcagttttg cgcatattac acacggctgt acggaaacat
     3361 atattgtgga gccgtctatg gatattccgg cctttgcagc ctgtacaacg gcattctgct
     3421 cggcatgtat agcacggcat aattcatgtt tctcccccga cggaacattc attttttgtc
     3481 ttaaacatcc cgtttcttca cagtgtttac atccgacagg tgcgccgtta taccctgtag
     3541 ccagtatcct tttgtcctta acaataacgg cacctaccgc acgtctcaaa caggtggaac
     3601 gggttttgac aagatgaacc acttgcatga aatattcatc ccaagaaggt ctttccatta
     3661 cttcgtacca aacaatctgt ctccggcatc acctaatccc ggtacgatgt aagcatcatc
     3721 attcaaggct ttatcaacac atgcacaata tatgtttaca tcgggatggt catcatggac
     3781 tttctttatg ccctcaggtg ctgcaattat actcataagc ttaatattct tgactcctct
     3841 ttgctttaag aaccctatag cagccgaggc agatcctccc gttgcaagca tagggtcaag
     3901 cagtataatc attctttcat gagcatcttc cggaagtttg cagtaatact cgaccggttg
     3961 caatgtctca tgatctctgt ataaccctat atgccctatc ttcgcggtgg gaatcaaagt
     4021 caacagaccg tcaaccatac ccaatcccgc tctcagtata ggaacaaatc ccacactttt
     4081 ccccgaaagc acctttacgg tagtttttgc tatcggtgtc tctacctcaa cctgctccag
     4141 cggcaactct cgcgtaacct catatcccat caatgtggat acttcggtta caagctctct
     4201 gaattcctta gtatttgtat tcttatccct cataatggat atcttatgtt gtataagagg
     4261 gtgatcgaat acgaatgcca tgtccatata attggttccg gacatacttc ctccttcttc
     4321 tatcctgcgg atcaatccgt tccttaattc gtattttcct tcaaggtaag tttcgttaag
     4381 ccatatgtct ataatcttat gcgccatatc tttcggagta attctgcctg caagcaccag
     4441 tatgttggca ttattgtggc gctttgccat acttgcctga tattcgtcca tacacaatgc
     4501 cgcccttata cccttgaact tgtttgccgc aatgctcata cctattccgg ttccgcatat
     4561 taaaatacct ctttcacact cgttcttctg aacggatgat gctactaaag aggcataaaa
     4621 aggaaaatga caggaatctt cgctgtaaga tccgaaatcc cgatacggta tgttcttttg
     4681 ctcgagatac gggagtatct cgcttttcag cgtaaaaccc gcatggtcac aacctatggc
     4741 taacatggga tatctcctct gtttttattt aaaggaattc tatcacaaat aataacatat
     4801 atccacacta aattaaaagc aatttatcaa attaccaaac tcaactatat atattaataa
     4861 acagttatca gcttattaat atatattcat cctctccgaa agaaactata tccccccttt
     4921 taactttaac aaaactttcc ggcttaatcc tatcaccgtt taagtaagtg ccgtatttgc
     4981 tgccaagatc ccgtacatat acggtatcat gggaatatct catacgggca tgtcttaagg
     5041 aagcaagttt gcccggaagt cggtaatcac aaatcaactt gagtcttccc accgttatgt
     5101 ctttatcggc aattattatg cagtcgtcgg tatctactat gtttttaaat acggcttttt
     5161 taggttcttc acaaactttc tcctgcacat caatatttcc gtcatgggaa tcctttatta
     5221 tcccgtttat atcaaaatgc tcagaagata aagcttcttt aatacttctc tcggtataat
     5281 taccttcggt tatttcgctg acaagctgtt taagaccgtt atttaagtcg tacttattga
     5341 aagggtccaa tatgtatttg aatgtttcat ccttgaatac atatatataa cgtgtatccg
     5401 gaactacata ttcggcatct atgagatagg agcctgttat ccgcatcata tcctttatac
     5461 tgtataaaag cttttttaaa agagcgggag aatatccctt ctttttaaga tataccgata
     5521 aacttgttcc gttaagcgtg tcgaatatca gttcatcacc tttattgtat
//
LOCUS       DAOPWC010000047         5514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14747_length_5514_cov_3.993222, whole genome shotgun sequence.
ACCESSION   DAOPWC010000047 DAOPWC010000000
VERSION     DAOPWC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14747_length_5514_cov_3.993222"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKJ65_01805"
     CDS             <1..302
                     /locus_tag="PKJ65_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014353331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40606.1"
                     /translation="VSDDIKDYIVRIVTATRNSVRLILGLSPRGSLALMRAARAYAAI
                     KGRDFVIPSDVKTVAPHVMSHRVMLKGHSLSYGMRGTMDVINEVLDTVPVPTEEV"
     gene            309..1463
                     /locus_tag="PKJ65_01810"
     CDS             309..1463
                     /locus_tag="PKJ65_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ40607.1"
                     /translation="MELILIVTSLVIFFVAEIVFFRFFVLRKIEYSLSFSQSEAYEGD
                     DIFIDEVINNNKSVAVPWLKADIHASKWLQFANTSSTIIDEERCVSSGFSLKGYQKVT
                     RRWYTRCAKRGVFTIVNTTLTAGDLLGLMQLSEAYPANTRLMVFPGVLRLEEAFFSNR
                     DLIGEMIVKRFIMDDPFIVKGTREYVQGDPLNRINWKTTARMQKLMVRENDFTAKKGI
                     TVLFNIKSYAMEFIDVKYKEVIELGIKVVNTVLEKGYGMGFPLRLGTNAGILSNPDDM
                     IFTDSLFGPEHMRHLKVILAELILKNHLDFPDFLERHYDDIYDTQVYIVSAYLNDEIM
                     EKADILRRNGNTVKFLILNYTSDATSNRGMEVFLLDRNTEFLKEGEYHGA"
     gene            1453..2865
                     /locus_tag="PKJ65_01815"
     CDS             1453..2865
                     /locus_tag="PKJ65_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40608.1"
                     /translation="MAPEKQIRRKILPLLGNIGSVGGLTFAIYYEIFPETVYHWLGFC
                     FLMMVIGTMAGVMIVDFYDNKGEGIVVADVDRVRLFLQPAYIYAVIPFVLLFVLGLIV
                     SRGFVEAFIGFALMPTIYFILGIGNGLFDYTQTANRKILYTALIPLIIAIFKAFVSGP
                     YGFYKDPVWIFGSLYMLFFMLTINRIKIEELFVLSRKVNVENKKTIRRRNDFLIMIFF
                     ALYLIIFVVRNTFTRISEWIFKNTIAVIGFIINFFYKMRKVIEVEQVQEVVEEGIPSE
                     APPLPPTMNPMAKTVLIVIASLTIAFVLYFLVKKIIQLIRWLIGKASDTLTGGKRIKR
                     DTENEEFEEIVEFTKDKARRKRQKTIRYKYSLRELKNLDTYKEKVRYLYGFALERLKY
                     KNVKISKADTPEEIVDKLGTYENGNKLVDNVFKDFTDEYINVRYGDKETGQDEDFMEK
                     ADAVDKGIENIKVKDEKATA"
     gene            2971..5361
                     /gene="lon"
                     /locus_tag="PKJ65_01820"
     CDS             2971..5361
                     /gene="lon"
                     /locus_tag="PKJ65_01820"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ40609.1"
                     /translation="MTKEKNNIKEIIENEDIKENPMKVEFTKIPLIPLRAVAVIPGMV
                     THFDVGREKSILALEKAMEENGQVFLVMQRDASIENPKRENMYEYGTIANVKQVLRLP
                     GRTCRILVEGIKRAKLEETVTVKGYYTAIVTIPEITEAIISANNILEAMHRKLTTLYE
                     EYVTLLGRISHEGYFNAVAADDIGVVADAMVGSMALKSEVVQSFIEELDGITRAEKLL
                     PLLVNEIEILKLERDLATKVKSTIDEKQKEYYLREQLKAIQEELGEYSEEVDENEMGE
                     YIKAIESKKYPEEVRAKLKKEMSRLSKMSPMSPEASVIRSYMETLIELPFGIETKEKL
                     DIKHVGRILERDHYGMNKVKERIIEYIAAKKLSNNFNSPILCLVGPPGTGKTSIVKSI
                     AESINRKYVRMSLGGIKDEAEIRGHRRTYIGSMPGRVVKAIKQAGSMNPLILFDEIDK
                     LGNDFRGDPASAMLEVLDAEQNNAFRDHYLEVSLDLSRVMFITTANTVETIPRALLDR
                     MEVIEVTGYTEEEKMHIANKYIIPKQLKKHGMTRKHLNISESALKKVISNYTRESGVR
                     NLEREIASLCRKAATRMQLDETDRVKLTVDNIEDFLGPQKYHYDTTFDKDEVGIARGL
                     AWTQVGGDTLTIEVNIMNGTGHVELTGRLGDVMKESAKAAIGYIRSQTDRLNINPDFY
                     KDKDIHVHVPEGAVPKDGPSAGITLATAIISALTNRKVRRDVAMTGEITLRGRVLPIG
                     GLKEKLSAACRANVRQVLMPMENMRDLAEVPKQITDNLEITPVSNMDDVLKNALAE"
BASE COUNT         1812 a          972 c         1324 g         1406 t
ORIGIN      
        1 cggtatcgga tgatatcaaa gactatattg taagaatagt aacggctacc agaaattccg
       61 taaggcttat cttaggctta agtccgagag gaagtctggc acttatgaga gctgcaagag
      121 cgtatgcggc tattaaagga agggattttg taataccttc cgatgttaaa acggtcgctc
      181 cccatgttat gagtcacagg gttatgctga aaggacattc gctttcttac ggtatgcggg
      241 gaaccatgga tgtcataaac gaagtattgg atacggtacc ggtacccaca gaggaagtat
      301 agtaaagtat ggaacttata ctgattgtca caagccttgt aatattcttt gttgcggaga
      361 ttgtattctt cagattcttc gtgttgagaa aaatagaata ttcgcttagt ttcagtcaga
      421 gtgaagcata tgaaggcgat gatatattta ttgatgaagt tataaacaat aataaaagtg
      481 ttgccgtgcc gtggcttaag gcggatatac acgcatcgaa atggctgcag tttgccaaca
      541 ccagttccac tattattgat gaggagcgat gcgtatcttc cggcttcagt cttaagggtt
      601 atcagaaggt aaccagaaga tggtatacgc gatgtgcgaa aagaggagta tttaccatag
      661 tcaataccac tcttactgcc ggtgatcttc tgggccttat gcagctttcc gaggcctatc
      721 ccgcaaatac cagacttatg gtatttccgg gcgtgttgcg cctggaggaa gcttttttct
      781 ccaacaggga cttaataggt gaaatgatag ttaaaaggtt cataatggat gatcctttca
      841 tagtcaaagg cacaagagaa tatgtgcagg gagatcctct gaacaggata aactggaaaa
      901 ccaccgcaag aatgcagaag cttatggtgc gggaaaacga ttttaccgca aaaaaaggta
      961 tcacggtact tttcaatatc aagtcgtatg caatggaatt tatcgatgtc aagtataaag
     1021 aggtaataga gctgggtatt aaagtggtca ataccgtttt ggaaaaaggt tacggaatgg
     1081 gctttccttt acgcttaggc accaatgcgg gaatactgtc caaccccgat gatatgatat
     1141 ttaccgattc gctgttcggc cccgagcata tgaggcatct taaggttata cttgcggaac
     1201 tgatattgaa gaatcatctg gattttcccg atttcctcga aagacactat gatgatatat
     1261 atgatacaca ggtatatata gtatccgcat atctgaatga tgagataatg gaaaaagcgg
     1321 atatattgag aaggaacgga aataccgtaa agtttctgat acttaactat acatcggacg
     1381 caacatcaaa caggggtatg gaagtgttcc tgttggacag aaatacggag tttttgaagg
     1441 aaggggaata tcatggcgcc tgaaaaacag ataagacgta agatactgcc ccttttgggg
     1501 aatatagggt cggtaggcgg tctgactttc gcaatatact atgaaatatt tccggagact
     1561 gtttaccact ggctgggttt ctgttttctg atgatggtta taggaactat ggcgggagta
     1621 atgattgtgg atttttacga taataaggga gagggaattg tcgtagcgga tgttgacaga
     1681 gtgcgactgt ttctccaacc cgcatatatc tatgcggtaa taccttttgt gttacttttt
     1741 gtgttgggac tgattgtctc ccgcgggttt gttgaagctt ttatcggttt tgctcttatg
     1801 cccaccatat acttcatatt gggtatagga aacggtttat tcgactacac tcagaccgcc
     1861 aacagaaaga tattgtatac cgcattaatt cccttaatta ttgctatttt caaagctttt
     1921 gtttcgggtc cttacggttt ctataaagat cctgtctgga tattcgggtc gctgtatatg
     1981 ttatttttca tgctgacaat aaacagaata aagatagagg aattgtttgt tctgtccagg
     2041 aaagtgaatg tggagaataa gaaaaccata agaagaagaa atgattttct cattatgata
     2101 ttctttgcac tttacttgat aatattcgtt gtacgtaata cattcaccag aatttcggaa
     2161 tggatattca aaaacaccat agccgtaata ggttttataa tcaacttttt ttataaaatg
     2221 cgtaaggtta ttgaagtgga acaggttcag gaggtagtcg aggaaggtat tccgagcgaa
     2281 gcgcctccgt tacctccgac aatgaatccg atggcaaaaa cggtacttat agtcattgct
     2341 tcactgacca tagcttttgt tttgtacttc ctggtcaaga agataattca gctcataagg
     2401 tggcttatag gcaaagcaag cgatacatta accggcggta agagaataaa aagagatacg
     2461 gaaaacgaag agtttgaaga gattgtggaa tttaccaaag ataaggcacg aagaaaaaga
     2521 caaaaaacca tccggtacaa gtattcgctc agagagctta agaatctgga tacatataaa
     2581 gagaaagtcc gttatctgta cggattcgca ttggaaaggc tgaaatacaa gaatgtaaaa
     2641 atatccaaag ccgatacacc ggaagagatt gtggataagc tgggcactta tgagaacggt
     2701 aacaaacttg tcgataacgt gtttaaggat tttaccgacg aatatatcaa tgtgcgatac
     2761 ggtgataaag aaacggggca ggatgaggat tttatggaaa aagccgatgc cgtggacaaa
     2821 ggcattgaaa acataaaagt caaggatgaa aaggcaacag cctgacttta atcttattgc
     2881 atattttgca taacttaaga atgtttgata gaatagcaga tgcacaaaaa taagctgtga
     2941 atgttttacg gcttgatgag gataaatgat atgacaaaag aaaagaataa tataaaagaa
     3001 ataattgaaa atgaagatat aaaggaaaac cctatgaaag tggagtttac caagataccc
     3061 cttatacccc ttcgggccgt tgcggtgata cccggtatgg taacccattt cgatgtgggc
     3121 agagagaagt ctattctggc actggaaaag gccatggaag aaaacggaca ggttttcctt
     3181 gtaatgcaga gagatgcttc aatagaaaat cccaagcggg aaaatatgta tgaatacgga
     3241 accatagcca atgtcaaaca ggtgttaagg cttccgggaa gaacctgcag aatactggtg
     3301 gaaggtataa aaagagccaa actggaagaa accgttacgg tcaagggtta ttatacggca
     3361 atagttacca tacccgaaat tacggaagcg atcatatccg caaacaatat tttagaagcc
     3421 atgcacagaa agcttaccac cctgtacgaa gagtatgtga ctctcttggg cagaatatcc
     3481 catgaaggat atttcaatgc cgtggctgcg gatgacatag gtgtggttgc cgacgcaatg
     3541 gtgggatcca tggcgttgaa atcggaagtg gtacagtcct ttattgagga gttggacgga
     3601 ataactcgtg cggagaaact gcttcctctt ctggttaacg agatagagat actgaaactg
     3661 gagagagatc ttgccactaa ggtaaaaagc accatagacg aaaaacagaa ggaatactat
     3721 cttcgtgagc aattaaaagc catacaggaa gaactgggtg aatacagcga agaagtggat
     3781 gaaaacgaga tgggcgaata catcaaggct attgaatcca aaaagtatcc cgaggaagtc
     3841 agagcaaagt tgaaaaaaga gatgagcagg ctttccaaaa tgtctcccat gtctcccgaa
     3901 gcttcggtaa tccgttcata tatggaaact ctcatagagc ttcctttcgg aattgagacc
     3961 aaagaaaagc tggatataaa gcacgtcgga agaatactgg aaagagatca ctacggaatg
     4021 aataaggtaa aggagaggat aatagagtac attgccgcaa agaaattgag caataacttc
     4081 aattctccca tattatgcct tgtgggaccc ccgggaaccg gtaaaacttc catagttaaa
     4141 tccatagccg aatctataaa tcgtaaatat gtacgcatgt ccttaggcgg tatcaaagat
     4201 gaagcggaga taagaggaca cagaagaaca tatatagggt ccatgccggg aagagtggta
     4261 aaagccataa agcaggccgg ttccatgaat cctctgatac tgttcgatga aatagataag
     4321 ctgggaaatg atttcagagg agatcccgca agcgccatgt tggaagtatt ggatgctgaa
     4381 cagaataacg cttttcggga ccactatctg gaagtatcgc tggatttaag ccgtgtaatg
     4441 ttcataacca ccgccaatac ggtggaaact attcccagag cattgttaga ccgtatggaa
     4501 gtaatagagg ttacgggata taccgaagaa gagaagatgc atattgcaaa taagtatata
     4561 atccccaaac agcttaaaaa acacggcatg acaaggaagc atcttaacat ttccgagtct
     4621 gctctgaaaa aagttataag caattacaca agagaatcgg gagtgagaaa tctggagcgg
     4681 gagatagctt ctctgtgccg aaaagcggca acaagaatgc aattggatga aaccgacaga
     4741 gtaaagctga cagtggacaa tattgaggat ttcttaggac cgcagaaata tcattatgac
     4801 acaacattcg acaaggacga ggtgggaatt gccagagggc ttgcctggac acaggtggga
     4861 ggagataccc ttactataga agtgaatata atgaacggta ccggacatgt ggaactgacc
     4921 ggaaggttag gcgatgttat gaaagaatcg gccaaagccg caataggata tatccgttca
     4981 cagactgaca ggttgaacat aaatcccgat ttctataaag acaaagatat ccatgtacac
     5041 gtaccggaag gtgccgttcc caaagacgga ccttcggcag gtataacttt ggctaccgca
     5101 attatttccg ctctgacaaa cagaaaggta agaagggatg ttgccatgac gggtgaaata
     5161 accttaaggg gaagagtact tcccatagga ggtctcaaag aaaaactttc cgcagcatgc
     5221 agagctaatg tgaggcaggt cttaatgccc atggaaaata tgcgggatct ggcggaagta
     5281 cccaagcaga taacggacaa tcttgaaata actccggtca gcaatatgga tgatgtgctt
     5341 aagaatgctt tggctgaatg atatatcagg cttactgtct ttgaatctta agcctgtctt
     5401 gcttccattt gccggaaaaa tagaaatacc cccctataat catggaaaag caggtggata
     5461 tgggaacggc atatcctaag ccgcccactc ccgtttcaaa aaccttggca aaaa
//
LOCUS       DAOPWC010000048         5508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_14770_length_5508_cov_2.884467, whole genome shotgun sequence.
ACCESSION   DAOPWC010000048 DAOPWC010000000
VERSION     DAOPWC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14770_length_5508_cov_2.884467"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKJ65_01825"
     CDS             <1..190
                     /locus_tag="PKJ65_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNZ40610.1"
                     /translation="TGPHEGIIGASRDTVIEKFLTGMPAYFTSQEGRGQLNAVILELC
                     EKTGKAISIVRINSVEKD"
     gene            174..653
                     /gene="lspA"
                     /locus_tag="PKJ65_01830"
     CDS             174..653
                     /gene="lspA"
                     /locus_tag="PKJ65_01830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017000562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNZ40611.1"
                     /translation="MLRRINFKNILWLFLSILIIAADQLSKFFIVSNFQLGDSIKIID
                     GFLYITHTRNTGAAWGIFGEHTEYLAIFSVVATIALVVMLLYMKRACGAFSLALIIGG
                     AVGNAIDRIRLGWVVDFIDAYIFGYDFPAFNVADSAITAGVFLLIIYMLFVHREMSP"
     gene            656..1618
                     /locus_tag="PKJ65_01835"
     CDS             656..1618
                     /locus_tag="PKJ65_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ40612.1"
                     /translation="MDNINDTCDEIILQDNVLVDELVFSADSDTGIRLDVYLTKYMED
                     YSRSFIQKIIREGQVTVNSLVRNASYSVKSNDVIKVIMPDILPSSIQPQNIPLDILYE
                     DDDIIVINKKRGMVVHPATGNYTDTLVNALLYHTKNSLSDINGVERPGIVHRIDKDTS
                     GVILACKNNYSHRVLTESFARHDIKREYTAIVKGYPDSDKGLINAPIARDRLNRLKMA
                     VDSDGKNAVTHFEVLEKMHGASKIKAVLETGRTHQIRVHMAFIGNPLLGDDIYGGKDK
                     RFTEGQLLHAYIRGFVHPVKKQYCEFTAPPDDYFKNAVKVLVNT"
     gene            1615..2301
                     /locus_tag="PKJ65_01840"
     CDS             1615..2301
                     /locus_tag="PKJ65_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNZ40613.1"
                     /translation="MSDAIYDRTIRLIGERAMQELRQKKVAIIGLGGVGSYVLEAFAR
                     AGIGYLILADYEKIEKSNINRQILALHSTVGKYKTEVALERVKDINPDIKVTVHNVFV
                     SKDNIKDIIPDDCDYVIDAIDTVSSKIDIIRYAKKNGINIISCMGTGNKIDNTGFKIA
                     PVEKTDTCPLARKVRKILSSEGITGVDVLYSVSKNQVSAPDLISGISYVPSTAGLIIA
                     GYVIRKLINM"
     gene            2315..3844
                     /gene="xylB"
                     /locus_tag="PKJ65_01845"
     CDS             2315..3844
                     /gene="xylB"
                     /locus_tag="PKJ65_01845"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNZ40614.1"
                     /translation="MNYLLGIDIGTSATKSVLFSEDGKAYASASYEYPLYQPNPGWAE
                     QDPDDWWEAVCFTCKKIITDTGIDSGLIKGVGISGQMHGLVMLDEQDRLLGRSIIWCD
                     QRTEKQAEYLEGKIGRDRLISITANPAITGFTAAKILWVMQNRPDLYKKAKKILLPKD
                     YILYMLTGEFSADVSDGSGMQLMDVRKRVFSKELCDALEIDTSLLGYMHESADAAGKV
                     TKKAAFLTALKEGTIVAAGGGDQACGAVGNGIVKEGIVSSTIGTSGVVFAHTDKLHID
                     KLGRIHTLCHAVPGCYHVMGVTQAAGLSLKWFRDNFCKEEIDAAAAKGTDPYVIMDKM
                     AKDITIGAGGLIFLPYLMGERSPHLDPFCSGVFFGITPTHSKAHFIRAILEGVVYSLK
                     DSVSILREMGIDVSQVRVSGGGAKSPLWKTMQADIYESDIAAVVNSEGSALGAAILGG
                     VASGVYPSVTKACELVITTKNRQKYNKSAANEYNKYYEIYKSLYPALKEEYRNLRLLL
                     Q"
     gene            3938..4993
                     /locus_tag="PKJ65_01850"
     CDS             3938..4993
                     /locus_tag="PKJ65_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNZ40615.1"
                     /translation="MTYTIGIDLTFIKDHKKAVGNEISALSIVEGLHNNRSGQDFIIF
                     ANQDNAKDLKSMYKSFNVITTAKGFMFYNKFLPQFVKKYGVDVLYYPQYVRFMNFRLK
                     TKIVITTHGLIKGDDFLLDKLRPAMIKRAAKVVAASDFLKNQLIKKRSIDPSKIITIP
                     NPLANMTKSVDVVFKKKNILAVGSDKPQKNIMLVVRAFEKLVPIIEHDLILVGDISGK
                     GEIFRYIVDNNISNRIIVTGRVSRETLFGYYKNTDLFVNTSTYEGFGLAPLEAMAFKA
                     PVVSTPIPSISELGNVQFDAALNYEDDEREVANKMLAVLKSEKNTEYLENRAKRILDY
                     YSLDSISKKYLQVFENV"
     gene            5046..5393
                     /locus_tag="PKJ65_01855"
     CDS             5046..5393
                     /locus_tag="PKJ65_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ40616.1"
                     /translation="MLNKDNRWVRYAEAIPWDEIEKIYASKFSNRGAPAKPLRKVLGA
                     YILKEEYNFSEARIIKEINENPYLQYFIGLNEYTDKVPVSASLIRSFSKRFTEQDKTE
                     IERLLKEARKSLK"
BASE COUNT         1791 a          923 c         1312 g         1482 t
ORIGIN      
        1 gacggggccg catgaaggca taataggggc ttcgagggat acggtaatag agaagtttct
       61 tacgggaatg ccggcatatt ttacaagtca ggagggaagg gggcaactca atgcggttat
      121 actggaatta tgtgaaaaga ccggtaaagc aataagcatt gtgagaataa acagtgttga
      181 gaaggattaa ttttaagaat attctatggc tgtttctcag tattctgatt attgcggcag
      241 atcagctgtc caagttcttt attgtaagca actttcagtt gggcgacagc attaaaataa
      301 ttgacggctt cttgtatatc acccacacca gaaataccgg agcggcatgg ggaatattcg
      361 gcgaacatac cgaatacttg gcaatatttt ccgttgtagc cacaattgcc cttgtagtta
      421 tgcttttgta catgaaaaga gcatgcggcg ccttttccct ggctctcata atcgggggag
      481 cggtaggaaa tgccatagac aggataaggt tgggctgggt tgtggatttc atagatgcat
      541 atattttcgg gtatgatttt cccgcattca atgttgccga ttccgctata acggcaggtg
      601 tctttctgct tataatctac atgctctttg ttcacaggga gatgtcaccg tgagtatgga
      661 taatattaat gatacttgtg atgagataat attacaggac aatgtcttgg ttgatgaact
      721 tgttttttca gcagattcgg atacgggaat aagactggat gtttatctca ctaagtacat
      781 ggaagattac tcacggtcat ttatacagaa gattatcaga gaaggacagg ttaccgttaa
      841 tagtctggta agaaatgcaa gttattctgt taaaagtaat gatgttatta aggtaataat
      901 gcccgacatt ttaccttctt ccatacaacc tcagaatatt cctttggata tattatatga
      961 agatgacgat ataatagtga taaataaaaa aaggggaatg gtggtgcatc ccgctaccgg
     1021 aaactatacc gatacccttg taaacgccct gctgtaccac acaaaaaaca gtttgagtga
     1081 tataaacggg gtggaacgtc cgggaatagt acacaggatt gataaagaca cttccggcgt
     1141 tattttggcc tgcaagaata actattcgca tagagtttta accgaatcat ttgcacgaca
     1201 tgatattaaa agggagtata ccgcaatagt taaaggctat cccgattccg acaaaggcct
     1261 tattaacgct cctatagcaa gagacaggct taacaggctg aaaatggcag tggattctga
     1321 cggtaagaat gcagtaactc attttgaagt attggagaag atgcacggtg catcaaaaat
     1381 aaaagcggtt ctggaaacgg gcagaaccca tcagataagg gtgcatatgg cttttatagg
     1441 gaatccttta ttgggtgatg acatttacgg aggtaaggat aaacggttta ccgagggaca
     1501 acttctgcat gcttatattc ggggctttgt acatccggta aagaagcaat attgtgagtt
     1561 taccgcaccg cccgatgatt acttcaaaaa tgcagtgaaa gtattggtga atacatgagt
     1621 gatgctatct atgacagaac aatccgcctt ataggggagc gggcaatgca ggagttgcgt
     1681 caaaagaaag tcgctataat aggactcgga ggagtgggtt cctatgttct ggaagctttt
     1741 gcaagagcgg gtatagggta cttgatactt gccgattatg aaaagattga gaagagcaat
     1801 ataaacaggc aaatattggc cttgcattcc accgtgggga agtataagac ggaggttgct
     1861 ttagaacgtg taaaagacat aaatcccgat attaaagtaa ccgtgcataa tgtttttgta
     1921 agtaaagata atataaaaga cataatacct gatgattgtg attatgtaat agatgcaatt
     1981 gataccgttt catcaaaaat tgatataatc agatatgcca agaaaaacgg cataaacata
     2041 atatcctgca tgggaacggg aaataagatt gataatacgg ggtttaaaat tgcacctgtt
     2101 gaaaagaccg atacctgtcc tttggcaaga aaggttagaa aaatattatc atccgaaggt
     2161 ataaccggtg tggatgtact gtattcggta agtaagaatc aggtaagcgc ccctgatctc
     2221 atatccggta tatcttatgt gccttcaaca gcgggactta tcattgcagg ttatgttata
     2281 cggaaattaa taaatatgta aacggaggtt ctttatgaat tatttattgg gaatagatat
     2341 aggtacttca gccacaaaat ccgttttgtt cagtgaagac ggaaaagctt acgcatccgc
     2401 atcatatgaa tatccgcttt atcagcccaa tccgggatgg gcggaacagg atcctgatga
     2461 ctggtgggaa gctgtatgct ttacatgtaa gaagattatt accgataccg gtattgacag
     2521 cggacttatt aagggcgtag gtatttccgg acagatgcac ggtcttgtca tgttggacga
     2581 acaagaccgc cttcttggac gctccataat atggtgcgat caaagaacgg aaaaacaggc
     2641 tgaatatctc gaaggtaaga taggaagaga cagattgata agcataactg caaatcccgc
     2701 cattacgggg tttacagcag ctaaaatatt atgggttatg cagaaccgac cggatttata
     2761 taaaaaggcc aaaaaaatac tgctgcccaa agactatatt ctttacatgc ttaccggtga
     2821 gttcagtgcc gacgtgtcgg acggttcggg aatgcagctg atggatgtca ggaaaagagt
     2881 attttccaaa gaattatgtg atgcactgga aatagacact tcattactgg gctatatgca
     2941 cgaatcggcg gatgcggcag gtaaagtaac aaaaaaggct gcttttttga ctgcacttaa
     3001 agaaggaact atagttgccg cagggggagg ggatcaggcc tgcggtgcag taggtaacgg
     3061 aatagtaaaa gaaggaatag tatcttctac aataggtaca agcggcgtgg tatttgctca
     3121 caccgataaa ctgcatattg ataagttagg aaggattcat accttatgtc atgctgtacc
     3181 cggatgttat catgttatgg gcgttacaca ggctgcggga ttgtcgttaa aatggttcag
     3241 agataatttt tgcaaagaag agattgatgc ggcggcggca aaaggaacag acccgtatgt
     3301 aataatggat aaaatggcga aagatataac aataggagca gggggattga ttttcctgcc
     3361 gtatctcatg ggagaacgat ctcctcacct ggatcctttc tgttccggag tgtttttcgg
     3421 tattactccc actcactcaa aagcgcattt tataagggcg atactggagg gagtggtgta
     3481 ttcgctaaaa gacagtgtca gtatattaag agaaatgggt attgatgtaa gccaggtaag
     3541 ggtttcgggg ggaggagcca aaagtcctct gtggaagaca atgcaggctg atatatatga
     3601 gagcgatatt gcggctgtgg tcaattccga agggtcggca ttgggtgcgg caatcttagg
     3661 aggtgttgca agcggagtat atccgtcggt taccaaggca tgcgagcttg taattacaac
     3721 taaaaacagg caaaaataca ataaaagcgc tgcaaatgag tataataagt actatgaaat
     3781 atataaatcg ctgtatcccg ctctaaagga agaatacaga aatttgcgcc tgttgttaca
     3841 gtgattatat tcaacagtgt atatttttta attgcataac ggctatattt aatatataat
     3901 ctgattgacc ctattttgta tgttaacgga gactattatg acctatacca taggtatcga
     3961 cttgacattt atcaaggatc acaaaaaggc tgtcggcaac gagatatccg ctttaagtat
     4021 tgttgagggt ttacacaaca acagaagcgg acaggatttc ataatatttg caaaccagga
     4081 taatgccaaa gacttaaaaa gtatgtacaa atcctttaat gttattacca ctgcaaaagg
     4141 attcatgttt tataataaat ttttaccgca gtttgtaaag aagtacggtg tggatgtttt
     4201 atactatccg cagtatgtac gttttatgaa tttccggctt aaaacaaaaa tagtcattac
     4261 aacacacggt ttaatcaaag gtgatgattt cttattggat aaactcaggc cggcaatgat
     4321 aaaaagagcg gctaaagtgg tagccgcctc ggattttctg aaaaatcaac tgataaaaaa
     4381 aaggagcatt gatccttcaa agattattac catacccaac cctttggcca atatgacaaa
     4441 gagtgtggat gtggtgttca aaaaaaagaa tatattggcg gtaggaagcg ataaacctca
     4501 gaagaatata atgctggtgg tgagggcatt tgagaaactt gtaccgatta tagaacacga
     4561 ccttatactt gtaggcgata tatcgggtaa aggcgagata ttccgttata tcgtggacaa
     4621 taatatttcc aacaggatta tagtaaccgg aagagtgagc agggaaacat tattcggata
     4681 ctataaaaat accgatttgt ttgtcaacac ttcaacatat gaagggttcg gattggctcc
     4741 gctggaagcc atggctttta aggctcccgt agtcagcacc cccatacctt ccatcagcga
     4801 attgggaaat gtgcagtttg atgccgccct gaactatgaa gatgatgaaa gggaagtggc
     4861 aaacaaaatg ctggcagtgc tgaaaagcga gaaaaatacg gaatatctgg aaaacagagc
     4921 taagagaatc ctggattact acagtctgga cagtataagt aagaaatatt tgcaggtatt
     4981 tgaaaatgta taggaaacag gcccgacaaa taactattta ttcttttgta acaccgttcg
     5041 gcggtatgct gaacaaggac aacagatggg ttcgatatgc cgaagcaatt ccctgggatg
     5101 agattgaaaa gatttatgct tccaagttca gcaacagagg tgcaccggca aagccgctcc
     5161 gaaaggtttt aggagcatat atactgaaag aggaatataa cttttccgaa gcaaggataa
     5221 tcaaggaaat aaacgagaat ccttatctgc agtattttat cggtctgaat gaatatacgg
     5281 ataaggttcc cgtcagcgca tcgttgatac gttctttcag caaaagattt accgaacagg
     5341 ataaaaccga gattgaacgc cttctaaagg aagcaagaaa aagtctcaag tagaaaagct
     5401 gtataaatgc tttacttagt agcaataaaa gtaatataat aggcggtgat aacatcgcct
     5461 tgtttggtat gtaagcaaaa gaaaaatagg aaaacggaga gatgaatg
//
LOCUS       DAOPWC010000049         5409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15138_length_5409_cov_3.454800, whole genome shotgun sequence.
ACCESSION   DAOPWC010000049 DAOPWC010000000
VERSION     DAOPWC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15138_length_5409_cov_3.454800"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /locus_tag="PKJ65_01860"
     CDS             <1..2024
                     /locus_tag="PKJ65_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ40617.1"
                     /translation="KDQKTVKELAIDFLLYGTLAEIFSRETGFHRGLGGSMHAFFIPF
                     GIYPNNAIVGGSADIAVGAALYKKVNKKSGMVIANLGDASLGCGPVWEGICFATMDQY
                     KTLWEGEHKGGLPIIFNFFNNFYGMGGQTMGETMGFDILARFGAGVNPEQMHAERIDG
                     YNPLAVIDAYKRKREIIEKKEGPVLLDVMTYRFAGHSPSDASAYRTKEEIDMWMEQDS
                     LIEYRQKLVKGGIAKEEEFEAILGDAASLIFKIYKMAIDPAITPKMDLVGNPKAIEDL
                     MFSNERIEKMDDRPVDALIPLEENTRYQQVQKKERRGIVDGVVVYKNKVFQIRDAIFE
                     AMIEKFYTDPTMVAYGEENREWGGAYAAYRGLCDSMPYHRLFNSSISEAAIVGSAVGY
                     AMAGGRAVVELMYCDFLGRAGDEVFNQLPKWQAMSAGILKMPVVVRVSVGAKYGAQHS
                     QDWTSLCAHIPGLKVVFPVTPYDAKGLLNAALSGTDPVIFFESQRLYDKGEMFHEGGV
                     PTEYYEVPIGEPDIKRAGKDITILTIGATLYTALDAADILKEKYGMEAEVIDARTVSP
                     FNYDTVVQSVKKTGRIILASDACERGSHLKDMATTISTLCFDYLDAPAVVVGSRNWIT
                     PAADYEKSFFPQPSWILDAIHQKIVPLKGHVSTMSFTDGEIVRLNRLGV"
     gene            2031..2792
                     /locus_tag="PKJ65_01865"
     CDS             2031..2792
                     /locus_tag="PKJ65_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNZ40618.1"
                     /translation="MNQRQLQIKRMLDDHSEVFLKQLKKIFSDVSEMTIRRDLILLED
                     KGYAVRTHGGAVSINRFKKAGEDETGYTVRQAENVEKKAIIARKASHFIEKDRSVYLD
                     AGSTVMGLANLLTDMKGCTFITSAVNTALKLASTDNSVILLGGTISPNTLACSGPEAM
                     QMLEIMNIDLAVMGASGFSADKGFSVSNMHDAKLKTRIIEKARKTIMLADSTKIEKDK
                     AYTFCRLKDVDIWVCDTLPDDKVLDKCKKDNVLVV"
     gene            2797..3960
                     /locus_tag="PKJ65_01870"
     CDS             2797..3960
                     /locus_tag="PKJ65_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40619.1"
                     /translation="MKKRLIALFLLFTLLIVLSSCKTFNEKNLDTDNITETSNVFNGD
                     INDRRFTRFRQSDGCSYSQFVETPEGIYYLKDFYVYFSSDGISKYVKLCSKPDCLHND
                     SDCNAYAGANLISYYDGYIYYVAIDKFNHYLFRMNMDGTGHVNIKPISTTNERYTGFF
                     EHGYLYYYKGTIFGLAGNTNTVIYKTPVFDNSEGIAVVDTEDNDIGIGKIFLFYPLDE
                     YMYFYTYSPDSECSVCRYSMETGQWSEFVNTEWCYENVFTDNDKVYWYLNGKGFYEYT
                     YETGVTEKVAESIGDGLYGISYNDKYIYAYQYFKEKYSSVFPMFYIFDRQYNLIDSVL
                     FDVEREDCFPPFYVTELEDYVILSSKINSNPSDYYLLKSEFGSGDIKVRKIGD"
     gene            3988..4551
                     /locus_tag="PKJ65_01875"
     CDS             3988..4551
                     /locus_tag="PKJ65_01875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ40620.1"
                     /translation="MKILFIRHGQTDWNIQERVQGSSDIPLNETGIKQAHAVKEMLKK
                     HSFDEVIVSPLERARHTASIVCEGRDVEMTIDRRIAERDFGKYEGVSYRQDTEYYKKV
                     WSLDMNNQLETVESYIAFYGRVVDFLEEIIKKHYGKTVLLVAHGGVSIAVAHYFKGAP
                     ENGDYRSYLLDNCEIAEYDETDIKKKL"
     gene            4548..>5409
                     /locus_tag="PKJ65_01880"
     CDS             4548..>5409
                     /locus_tag="PKJ65_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNZ40621.1"
                     /translation="MIDFNVSKSRYVRAVQCIKMYWMDRVKPEEFDYSVMDEAVLETG
                     NEVGELAQKLFDNVSVVEYSDDKSQMINRTSEYIRLGKRYIAEASFAYKGRFLSVDIL
                     EVCDDGVIINEVKSSTSVKDVYLDDAAYQSYVLSLSGFNVKRVNVITLNSSYVRRGEL
                     DLTQLFSVTDVTCQSFSRHNVVEEHLESMSQLDFEIEPDMEIDNYCFEPYPCGYFGYC
                     TKHLPEENIFALKNVFRKDKLSMYRSGIYSFSDVINERVVFSRLRPVVKDQLLKEKIV
                     DKKEIGRFVSK"
BASE COUNT         1720 a          921 c         1296 g         1472 t
ORIGIN      
        1 agaaagacca gaaaactgta aaagaactgg caatagactt tctgctttac ggaacattgg
       61 cggaaatatt ctccagagag accggtttcc acagaggatt gggcggctcc atgcatgcct
      121 tctttattcc gttcggcata taccccaaca atgcaatagt tggcggaagt gccgatatag
      181 ccgtaggagc ggctctttac aagaaggtta acaaaaagag cggtatggtt atagccaact
      241 taggtgatgc ttcattgggt tgcggaccgg tatgggaagg aatctgtttt gccacaatgg
      301 atcagtacaa aacattatgg gaaggcgagc ataaaggcgg attgcccata atattcaatt
      361 tcttcaataa cttctatgga atgggcggac agacaatggg tgaaaccatg ggctttgata
      421 ttcttgcccg tttcggagcg ggagtaaacc ccgagcagat gcatgccgag agaattgacg
      481 gatacaatcc tttggcggtt attgatgcgt ataagagaaa gagagagatt attgaaaaga
      541 aagaaggacc ggttcttctg gatgtcatga catacagatt tgcaggacac tctccgagtg
      601 atgcttcggc atacaggaca aaggaagaaa tagatatgtg gatggaacag gattcactta
      661 tcgaatacag gcagaaactt gtaaaaggcg gaatagcaaa agaggaagag tttgaagcaa
      721 ttctaggcga tgcggcttca ctgatattca agatatataa aatggctatt gatcccgcta
      781 taacacccaa gatggatctt gtgggtaatc ccaaagctat tgaagacctt atgttctcca
      841 atgaaagaat cgagaaaatg gatgacagac cggttgatgc tctcatacct ttagaagaaa
      901 atacccgtta tcagcaggtt cagaagaaag agagaagggg tattgtagac ggagtggtgg
      961 tttacaagaa taaagttttc cagataagag atgcgatatt cgaagcaatg atagagaagt
     1021 tctacacaga tcccactatg gttgcatacg gtgaggaaaa cagagaatgg ggaggagcat
     1081 atgccgcata cagaggatta tgcgattcca tgccctatca cagactgttc aactcctcca
     1141 taagtgaagc cgcaatagta ggctctgcgg taggatatgc aatggcgggc ggaagagcgg
     1201 tagtggaact tatgtattgc gatttcttgg gcagagcggg agatgaagta ttcaaccagc
     1261 ttcccaaatg gcaggccatg agtgccggaa tactcaaaat gccggtagtg gtaagagtaa
     1321 gtgtaggcgc caaatacggt gcgcagcatt cacaggactg gaccagttta tgtgcacata
     1381 tcccggggtt gaaggtagta ttcccggtaa caccttacga tgctaaaggt ctgctaaatg
     1441 ccgctctatc gggaaccgac ccggttatat tctttgaaag ccagagactg tatgacaaag
     1501 gcgagatgtt ccatgaaggc ggagtaccta ctgaatatta tgaggtaccg ataggagagc
     1561 ccgacataaa gagagcgggt aaggatataa ccatactgac aataggtgca acactttaca
     1621 cggctttgga tgcagccgat atactgaaag aaaaatacgg aatggaagcc gaagtaattg
     1681 atgcaagaac cgtttcaccg tttaattacg acacggttgt tcaatcggtt aaaaagaccg
     1741 gaagaattat acttgcttcg gatgcctgcg aaagaggatc tcaccttaaa gatatggcaa
     1801 cgacaatttc cacactgtgt tttgattatc tggatgcacc tgcagtagtt gtaggatcca
     1861 gaaactggat tacaccggca gccgattatg agaagtcttt cttccctcaa ccgagctgga
     1921 tattagatgc catacatcag aagattgttc cgttaaaagg tcatgtttcg acaatgagct
     1981 ttaccgacgg tgaaatagtc agattgaaca gattaggggt gtaagttaag ttgaatcaga
     2041 gacaattaca gataaagcgg atgctggatg atcattcaga ggtttttctc aagcagctga
     2101 agaagatttt ctccgatgtc tcggaaatga ccatacgccg tgatttgata ctgctggaag
     2161 ataagggata tgcggtgcgg actcacggcg gagcggtcag tataaaccgt tttaaaaaag
     2221 cgggggaaga tgagacaggt tatactgtaa gacaggcgga gaatgtggag aaaaaagcca
     2281 tcatcgcccg aaaagcctcc catttcattg aaaaagaccg atcggtatat ttggacgcgg
     2341 gaagtaccgt tatgggttta gccaatcttc ttaccgatat gaaaggctgc acctttataa
     2401 cttccgcggt aaacaccgct ttgaaacttg cttccaccga taactcggtt atattgctgg
     2461 gaggaacgat aagccccaat acattggcat gttccggtcc ggaagccatg cagatgttgg
     2521 agataatgaa cattgattta gctgtaatgg gagcttcggg attttccgcg gataagggtt
     2581 tttccgtttc caatatgcat gatgccaaat taaaaaccag aattatcgag aaggccagaa
     2641 aaaccataat gcttgcagat tctaccaaaa tagaaaagga taaagcatac actttctgcc
     2701 ggcttaagga tgtggatatt tgggtttgcg acaccctgcc cgatgataaa gttttagata
     2761 aatgcaaaaa agacaatgtt ctggttgtgt aaccgcatga aaaaaagatt aatcgcacta
     2821 tttctgttat ttactctgct tatagtatta tcctcctgta aaacttttaa tgagaagaat
     2881 cttgatacgg ataatatcac tgaaacaagc aatgttttta acggtgatat aaacgacaga
     2941 agatttacgc gtttccggca gtcggacgga tgttcttatt cacagtttgt tgagacaccc
     3001 gagggaatct attatctcaa ggacttctat gtctatttca gttccgacgg tatttcaaaa
     3061 tatgtaaaat tatgctcaaa acctgattgt cttcacaatg attctgattg taatgcttat
     3121 gcaggcgcaa acttaatctc gtattatgac gggtatatct actatgtagc aattgataaa
     3181 tttaatcatt acctgttcag aatgaatatg gatggcaccg gtcatgtcaa tataaaaccg
     3241 atttccacta caaatgaaag atataccggt ttttttgaac atggctattt atattactac
     3301 aaaggaacga ttttcggttt agccggaaac actaataccg tgatatacaa aacgccggtt
     3361 tttgacaaca gtgaaggaat agcggtagta gatactgaag ataatgatat cggtatcggt
     3421 aagatttttt tgttttatcc gttagatgaa tatatgtatt tttatactta ttctcctgat
     3481 tctgaatgct ccgtatgcag atattcaatg gagacaggac agtggagtga atttgtaaac
     3541 accgagtggt gttatgaaaa tgtatttacc gataacgata aagtatattg gtatctgaac
     3601 ggtaaaggat tttatgaata tacctacgaa acaggtgtaa cagaaaaagt agcggaaagt
     3661 ataggcgacg gcttatatgg aatttcgtat aatgacaagt atatttatgc atatcagtat
     3721 tttaaagaaa aatattcatc tgtttttccc atgttttaca tctttgacag gcagtacaat
     3781 ctgatagaca gtgtattgtt tgatgtggaa agagaggatt gctttcctcc tttttatgta
     3841 acggaactgg aggactatgt tatcctgtca tcaaaaataa acagtaaccc ttccgattac
     3901 tatctcttga aatcggaatt cggcagcggg gatataaagg tgaggaaaat cggtgactga
     3961 tagtccgttt tatgttatca tattcctatg aagattctat ttatacggca cggtcagacc
     4021 gattggaaca tacaggaaag agtacagggc tcttcggata taccacttaa tgaaacggga
     4081 ataaaacagg cacatgcggt taaagagatg cttaagaagc atagctttga tgaagtgata
     4141 gtttcgcctt tggaaagagc aagacatacg gcatccatag tatgtgaagg cagagatgtg
     4201 gaaatgacta tagaccgcag gattgcggaa agagacttcg gtaagtacga gggggtttca
     4261 tacaggcagg ataccgaata ttataaaaaa gtatggtcac tggatatgaa taatcagctt
     4321 gagactgtgg aatcatatat tgcgttttac ggaagagttg tggattttct tgaagaaata
     4381 attaaaaagc attacggcaa aaccgtctta ctggttgcac acggcggagt atcgatagcc
     4441 gttgcacatt atttcaaagg tgcccctgaa aacggagatt accgcagtta tttattggat
     4501 aactgcgaga tagcggaata tgatgagacg gatataaaaa agaaattatg atagatttca
     4561 atgtttcaaa atccagatat gtaagagcgg tacagtgtat aaaaatgtac tggatggaca
     4621 gagttaagcc ggaagagttt gactattccg taatggatga agcggtgctg gaaaccggca
     4681 atgaagtggg ggaattggcg caaaagcttt ttgataatgt atccgttgta gaatactcgg
     4741 atgataagtc gcaaatgata aataggacaa gtgaatatat caggctgggt aaaagatata
     4801 ttgccgaagc ttcatttgcc tataaaggac gctttttgag cgtggatata ttggaagtat
     4861 gtgatgacgg cgttatcatc aatgaggtaa agagttccac aagtgttaaa gatgtatacc
     4921 ttgatgatgc cgcatatcag agttatgttc tgtcactttc cggtttcaat gttaaaaggg
     4981 taaatgtaat aacactcaat tcaagttatg tcaggagagg cgagctggat ttgactcagc
     5041 tgttttcggt cactgatgtg acttgtcagt ctttttcccg ccataatgtt gtagaggaac
     5101 atttggaatc catgtcacaa ctggattttg aaatcgaacc ggatatggaa atagataact
     5161 actgttttga accttatcct tgcgggtatt tcggttactg cacaaagcat cttcccgaag
     5221 aaaatatatt tgcacttaag aatgttttca gaaaagacaa gcttagcatg tatcgtagcg
     5281 gcatatattc cttttccgat gttattaatg aaagagttgt attttcacgg ctgagaccgg
     5341 ttgtaaaaga ccagcttctg aaagaaaaga ttgtagataa aaaagagatc ggtcgatttg
     5401 taagcaagt
//
LOCUS       DAOPWC010000050         5349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15377_length_5349_cov_3.786929, whole genome shotgun sequence.
ACCESSION   DAOPWC010000050 DAOPWC010000000
VERSION     DAOPWC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15377_length_5349_cov_3.786929"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..772)
                     /locus_tag="PKJ65_01885"
     CDS             complement(188..772)
                     /locus_tag="PKJ65_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40622.1"
                     /translation="MIVYYHIPEDKAKDAISFGMKLSEYGNFLINIGNGPKNVITAYL
                     CPADDIKRYNRSGYTCLRIDFPMEKTYIMEGVYLETENTEALKASLTKAEEYKLGSYR
                     KPLALITTTVFAERIKVLGKFMDVPMVVDDSEELYLKNALLELEEGDSGYYEKALYGY
                     LTVTKEARMLYEKNGYVEYILNKRKYIARKPDGK"
     gene            complement(769..1872)
                     /locus_tag="PKJ65_01890"
     CDS             complement(769..1872)
                     /locus_tag="PKJ65_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HNZ40623.1"
                     /translation="MYPQTDIKSYMPVKILFGNNCVFNNKDIFKQTGKFPFIVTGKSG
                     AVKSGAFDDVISVLNGGPYILYDRVEENPKVSDMEDTAIISKKFRCDYIIAIGGGSAL
                     DAAKAIAVLAVNNMSGMDLFSKKADAALPVIAIPTTSGTGSEVTPYSILTVDEWQTKK
                     SFAGDCIYPKIALLDPKYTFSLPIDYTISTAFDAVSHLFESYFSLKSNEMSKMYSQEG
                     IRAFAKAIDAIKNRKFDEQSRYYLMYASMLGGIAISHTGTTAMHAMGYSMTYFHGLPH
                     GFANLYFAEPYFRHMMKQEEQKTKTVTDLMGMTDLQSMINFFSDSIQRPKLNKEQCER
                     YATLAMMQSSIKSTPGNLTKEDVVNMFKEAGRA"
     gene            complement(1856..2449)
                     /locus_tag="PKJ65_01895"
     CDS             complement(1856..2449)
                     /locus_tag="PKJ65_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40624.1"
                     /translation="MRQDKKTAIAIITVAFIMSLMFCTTAQTADLVIASVEYVDAKIA
                     EVRALIQSSGTPSGGTTPVTPVDNAKITELETALGNALKEMDALRSRMDFLDVTVRDM
                     SDNYGMQVLHVYKDDVIYALGTSELILRSGSAIVIGNANNEGLSDATTGENLNPGVSL
                     GKDHFVVIPRGDGRGIKITSGESFIMYRGSFSYVPAN"
     gene            complement(2525..3670)
                     /gene="alr"
                     /locus_tag="PKJ65_01900"
     CDS             complement(2525..3670)
                     /gene="alr"
                     /locus_tag="PKJ65_01900"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ40625.1"
                     /translation="MEKPDRVWVEINLDNIAHNIAAIRKKTGDVKVAAVVKADGYGHG
                     AVEIAQTLCENGVYMLVVATLDEGISLRRNGITAPILVLGYICPEKIEQAIDNNLAAT
                     ITNSDMARKYSSVAKEAGKTLYAHIKIDTGMHRVGFAYDDKQSILSALALCNIHYSGI
                     FSHFSTADEEDKTYTNMQYERFSGVLDSVKKAGYDIALKHICNSGAIILHQDKYMDMV
                     RPGIIIYGLYPSQYCKNHDMSDLRCAMSFKSRIIQIKKVKKGQPISYGNSYITERDSI
                     IATIACGYADGYSRVLSQKTHVFINNRRAPIVGNICMDMCMADITGLKGISVNDEVEL
                     FGPNIPVEHLAAICSTINYEYICKIGARVPRVYIKEGNMIKINNYLV"
     gene            complement(3657..4502)
                     /locus_tag="PKJ65_01905"
     CDS             complement(3657..4502)
                     /locus_tag="PKJ65_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNZ40626.1"
                     /translation="MKIVEYKESDISDFFPLRKDISCKSDYGRFLVIGGSPGMTGALR
                     LCVDACYRSGAGLVYYTAPEKDLLLYDLLTVEGITLPAEDILSFIGDKDCICIGCGLY
                     NDEENLILLKKVLENSKCNIVADAAALRMLAEHKELLKRHGNRMTVLPHPGEMSHLTG
                     LSVADIQKDRINTAYKFYEVYGCITVLKGNRTVVAGNDSVYINNTGNPGMATAGSGDV
                     LAGMVTAYSVMSNDMFKNACMAVYVHGLAGDMAAKDLGVISMISSDIIRYIPQAHKEV
                     ACGKA"
     gene            complement(4559..5020)
                     /locus_tag="PKJ65_01910"
     CDS             complement(4559..5020)
                     /locus_tag="PKJ65_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNZ40627.1"
                     /translation="MLKQYLYILAISAVPVIEQRGAIPLGILLYKLDPVLVTLVSYVG
                     SLIPMFFVIYCFTPIFKWLSTKKILKWFYDFVDNKVRKGKEKVERYEVIGLTIFVAIP
                     LPMTGVWTGSAVAAFMQMNKKKAFISVSIGAAISAIIITIICYVAPKLLGY"
     gene            complement(5014..>5349)
                     /locus_tag="PKJ65_01915"
     CDS             complement(5014..>5349)
                     /locus_tag="PKJ65_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNZ40628.1"
                     /translation="GLFGEGIDLVGEMLSGVAIIGPGLPMLCPERELIKKYFDDIDMN
                     GFDYSYTFPGMNRVLQAAGRVIRSETDTGFVLLIDSRFGTAKYRSLFPKWWNCSNMYN
                     SFEEDGFLC"
BASE COUNT         1379 a         1301 c          972 g         1697 t
ORIGIN      
        1 ataaccacgg aaaaccatgc tccgtcgctg ttgtcggata ggaaatccct gcctaaccgt
       61 cctctgccct gcaactgtct ttccgcctgt attaccgtaa ttttcttact gaaagactgc
      121 gcattatctt ttgcatatgt attggtggaa tttatatatg gaagtttaat gatttccata
      181 tcgcacatca cttgccatcc ggctttcttg ctatgtattt tcttttgttc agtatatatt
      241 caacatatcc gttcttttca taaagcatac gcgcttcttt tgtgaccgta agatatccgt
      301 ataaggcttt ttcataatat ccgctgtcgc cctcttccaa ctccagcagt gcatttttaa
      361 gataaagttc ctccgagtca tccacaacca tgggtacatc catgaatttt cccagcacct
      421 ttattctttc ggcaaatacc gtggtggtga taagagccaa aggctttcta tagcttccaa
      481 gcttatactc ctccgctttg gtcaaagaag ccttgagtgc ctcggtgttt tccgtttcca
      541 gatagactcc ctccattata tatgtctttt ccatgggaaa atcgatacgc aagcaggtat
      601 agcccgacct gttatatctc tttatatcat ccgcaggaca cagatatgcc gtgataacat
      661 ttttcggtcc gttgccgatg tttataagga aattgccgta ttccgaaagt ttcatcccga
      721 aagatatggc gtcttttgct ttgtcttcgg gaatgtgata atagactatc atgccctgcc
      781 tgcctcttta aacatattta caacatcctc ttttgtcagg ttgcccggtg tagatttaat
      841 gctggactgc atcatggcca gcgttgcata gcgctcacac tgttctttgt tcagtttcgg
      901 tctttgtatg ctatccgaaa agaagtttat cattgactgc aaatcggtca tacccattaa
      961 atccgtaacc gtttttgtct tttgctcttc ctgtttcatc atatgcctga aataaggttc
     1021 ggcaaaatac agattggcaa atccgtgcgg cagtccgtgg aaatatgtca tggaataacc
     1081 cattgcatgc attgcggtgg taccggtatg cgatattgct attccgccca acatggatgc
     1141 atacataaga taatatctgc tttgctcatc aaacttcctg ttttttattg catcaatggc
     1201 ttttgcaaaa gcacgtatgc cctcttgcga atacattttt gacatttcat tactctttaa
     1261 ggaaaagtaa ctttcgaaaa gatgacttac cgcatcgaaa gcggtggata tggtatagtc
     1321 gataggtaaa ctgaaggtat atttcggatc taacagtgca atcttgggat atatgcagtc
     1381 gccggcaaaa ctcttttttg tctgccactc atccacggtc agtatggaat aaggagtgac
     1441 ctcgcttccg gtcccgctgg ttgtcggtat ggctattaca ggtaaagccg catccgcctt
     1501 cttgctgaaa agatccatgc cgctcatgtt attaacggcc agcaccgcta tggcttttgc
     1561 cgcatccaaa gcggaacctc cgcctattgc aataatatag tcgcaacgga attttttact
     1621 gataattgcg gtatcttcca tgtcggatac cttgggattc tcttccaccc tgtcataaag
     1681 aatatacggt ccgccgttta aaacagatat cacatcatca aaagcgccgc tttttacggc
     1741 tccgcttttt ccggtcacaa taaagggaaa ctttccggtc tgcttaaata tatctttatt
     1801 gttaaagacg caattgtttc cgaaaagtat ctttaccggc atataacttt tgatatcagt
     1861 ttgcgggtac atatgagaag gaacctctgt acataataaa actttcaccc gatgtaatct
     1921 taatacctct gccgtcgcct ctgggtatta ccacaaagtg atctttaccc agcgataccc
     1981 cgggattgag attttctccc gtggtggcat cgcttaaccc ttcgttgttt gcatttccta
     2041 ttactattgc gctgccgctc cgcaagataa gctccgaagt acctaatgcg tatattacat
     2101 catctttata aacatgaaga acctgcatgc cgtaattatc cgacatgtct ctgacagtta
     2161 catcaagaaa atccattctg gatcttaaag catccatttc ctttaacgcg ttgcccaaag
     2221 ccgtttccag ctcggttatt ttagcattat ccacgggggt aaccggtgtt gtgcctcccg
     2281 acggcgttcc cgaagactga ataagagccc ttacctctgc tatctttgca tccacatatt
     2341 cgacacttgc aattacaaga tcggcagtct gtgccgtggt acagaacata aggctcatta
     2401 taaatgcaac ggttattatt gctatcgcgg tttttttatc ctgtctcatc ttttgttctc
     2461 ctctttatac ggttttattt ctttttacat tatacataac tacctgaata acggtcaatc
     2521 aatgttatac aaggtagtta tttatcttta tcatattgcc ctcttttata tagactctcg
     2581 gtactcttgc acctatctta caaatgtact cgtagtttat tgtggagcag attgccgcca
     2641 gatgttctac cggtatatta ggaccgaaca actctacctc atcattaacc gatatacctt
     2701 tcagtccggt aatgtccgcc atacacatat ccatgcatat attgcctact atcggagctc
     2761 ttcggttgtt tataaacaca tgggtttttt gtgaaagcac cctggaatag ccgtcggcat
     2821 atccgcatgc aatggtggca atgatactgt ctctctcggt aatataactg tttccgtaac
     2881 ttatcggctg accttttttc actttcttaa tctgtattat tctggacttg aaactcatag
     2941 cgcaccgcaa atccgacatg tcatgatttt tgcaatactg cgaaggatac agaccgtata
     3001 tgattattcc cggtcgcacc atgtccatat atttgtcttg atgcagtatt atcgctccgc
     3061 tgttacatat gtgcttcaaa gctatatcgt aacccgcttt tttcacactg tctaaaacac
     3121 cgctaaatct ttcatactgc atatttgtat atgttttatc ttcttcgtcg gcagtggaaa
     3181 aatgtgagaa tatcccgctg taatgaatat tgcaaagagc cagtgcggaa agaatagatt
     3241 gcttgtcatc atatgcaaaa cccaccctgt gcattccggt atctatcttg atgtgtgcat
     3301 acagcgtttt tcccgcttct tttgcaaccg aagagtactt ccttgccata tccgagttgg
     3361 taattgttgc cgcaaggtta ttgtctatcg cctgctcaat cttttccggg catatatagc
     3421 ctaacaccaa tatgggagcg gttatgccgt ttcttcgtaa acttatccct tcatcaagag
     3481 ttgccaccac cagcatatat acgccgtttt cgcaaagagt ctgggcaatc tccacagctc
     3541 cgtgtccgta accgtcggct tttaccacgg cggctacctt cacatcacct gttttttttc
     3601 ttatcgcagc gatgttgtgt gctatgttat ccagatttat ttccacccac actctgtcag
     3661 gcttttccac atgccacctc cttgtgtgct tggggtatat accttattat atccgatgaa
     3721 atcatactta tcacgcccaa gtctttagca gccatatctc ccgccagacc gtgtacatat
     3781 accgccatgc aggcattttt gaacatgtca ttactcataa ccgagtatgc cgttaccata
     3841 cctgcaagaa catcccccga tcctgcggtg gccattccgg gatttcccgt attgttaata
     3901 tacacggaat cattccccgc aaccacggtc cggttgcctt ttaacaccgt tatacatccg
     3961 tacacttcat aaaatttata tgccgtatta attctgtctt tttgaatatc cgctaccgac
     4021 aaccccgtaa gatgagacat ttctccggga tgcggtaaca cggtcattct gttgccgtgc
     4081 cgtttcaaca gttctttatg ttccgcaagc atcctcagcg ccgcagcatc ggctactatg
     4141 ttgcatttcg agttttccaa tacttttttt aacagtatca ggttttcttc atcgttatac
     4201 agaccgcatc caatgcatat acagtcttta tcaccgataa acgaaagaat atcttctgcc
     4261 ggtagggtaa taccttccac ggtaagaagg tcatataaca gtaagtcttt ttcgggcgcc
     4321 gtatagtaca caagtcccgc tccgcttctg taacaggcat ccacacataa tctcaacgct
     4381 ccggtcatgc cgggtgagcc gcctattaca agaaacctgc cgtaatccga tttacagctt
     4441 atgtcttttc ttaacgggaa aaaatccgat atatcgcttt ctttatattc aacaatcttc
     4501 atagaggtca ttatatcacc aataaagtga ttagttaaat aagttagtta tggaaaaatc
     4561 aatatcctaa aagcttaggg gctacatagc atattatagt tatgataata gcggatatgg
     4621 cggctcctat actgacactt ataaaagcct tctttttgtt catctgcatg aatgcggcta
     4681 cggcactgcc ggtccacact ccggtcatgg gaagaggtat ggcaacaaat atggtaagtc
     4741 ctattacttc atatctttcc accttttctt tgcctttcct caccttgtta tccacaaaat
     4801 cgtaaaacca cttaagaatc ttttttgtac tcagccactt aaatatggga gtaaagcagt
     4861 agattacaaa aaacattgga atcaaagagc ctacatagga aacaagtgta acaagaaccg
     4921 gatccaattt atacagtagt attcccaacg gaattgcgcc tctttgctct attacgggaa
     4981 ccgctgaaat cgccaatatg tacaggtatt gttttaacat aaaaaaccgt cctcttcaaa
     5041 actattgtac atattactac aattccacca ctttggaaat agcgacctgt attttgccgt
     5101 cccgaaacgg gaatctatga gcagcacaaa gccggtatcg gtttcgcttc gtattactct
     5161 tcccgcagcc tgaagcaccc tgttcattcc gggaaacgtg taagaatagt caaaaccgtt
     5221 catatcaatg tcatcaaaat acttctttat aagttccctt tccggacaca gcatgggaag
     5281 tccgggacct attatcgcca caccggaaag catttctccc acaaggtcta ttccttctcc
     5341 gaaaagtcc
//
LOCUS       DAOPWC010000051         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15401_length_5340_cov_2.919395, whole genome shotgun sequence.
ACCESSION   DAOPWC010000051 DAOPWC010000000
VERSION     DAOPWC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15401_length_5340_cov_2.919395"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1123)
                     /locus_tag="PKJ65_01920"
     CDS             complement(50..1123)
                     /locus_tag="PKJ65_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40629.1"
                     /translation="MKKVCLLIICLFILSFILTGCDNQPWINMVDTLMQGDYRKANKQ
                     YEKIEKNYVHAMNTSEYVYQYIENIVASFKQDKTSYETAEKKLNLFSLLEMPLYNLED
                     AKSEVERIQASRTAYNQARELYLAELYYDAHNKFKLVEIDDSRNYDKAQEYLLSIVYV
                     LLYREEYYKAADVILSVDINSNTVPKLAEYSVDLAIRLLYYKDDMDRAYELNDKLIKL
                     LIRMEPGVKDAIKEWSRKLESIYSRPEYAAVCISFLSDDMEDMFYKLEQTDLRLETQK
                     TDYAFIILKSIPVRQDTIGYIAPRMTEILYQYISQGKPDYAQSVVDIIFSEIDDDRMV
                     KSLVTYIQDNIKNKEYLNKIDMA"
     gene            complement(1168..1878)
                     /locus_tag="PKJ65_01925"
     CDS             complement(1168..1878)
                     /locus_tag="PKJ65_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ40630.1"
                     /translation="MTNISIWGDSVLRGVVYDDNAGKYVFLQNSCIPETAKNFNITYV
                     NNSKYGMTAPKALERMKKNLEENDIKPDFALIELGGNDCDFNWKEVASNPDINHDPNT
                     PIKVFDKTIRQMVTMFREKGIEPVLTNLHPIDPVRYLDWITKDGTSKEAILKWLKDVF
                     KIYRFQELYSLTIESISKQLNVRLVDIRKAFLNARNLMELLCRDGIHPNEKGHEIIAN
                     CLIEYISDYKKEVLQGAV"
     gene            complement(1987..2661)
                     /locus_tag="PKJ65_01930"
     CDS             complement(1987..2661)
                     /locus_tag="PKJ65_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNZ40631.1"
                     /translation="MNVADYILILIIIVSMILGGIRGIIMSAYGLLTTILSIFLSFKF
                     AQPIRDLLVGTSFYNNIHEKIEHMAEALVPNLDSVEEAIIATVNSLPFPDDIKIWLIE
                     RIDFSQTTTKAEAMGGLVANITDLIIAIIIGIILFVLLLIIFKLLRGLVKKISELPVI
                     KQVDAIVGAIIGAVSGVLLIYIIFLIISGFQTAEIMSVVHETIQKSKLASVLYNNNLL
                     LELFKK"
     gene            complement(2723..3829)
                     /locus_tag="PKJ65_01935"
     CDS             complement(2723..3829)
                     /locus_tag="PKJ65_01935"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HNZ40632.1"
                     /translation="MAKKRKKKAYWIIIVVLLAVLVAIGFIFFRTEILRFFKADVVAK
                     DAGVQYTIPIATGSVVASENGYIYIYTDSGLSVCKSNGEFLRSYLLSYTDTKIAYSDT
                     HVVVYDKGATSYTVFCEGKKVDEYTAKTAITYVFAFDEYMFLLCEGLQGYLGAVYCST
                     YQTNLIIPDGYKNDIQYSGRYPVYLDMFDDKEQFAVVSCVPDDMSKTHIEVFEIGKPT
                     PIAGITLDGFLPKTVCLQDGRFLVANDTDVLLIDKVMEKTSVFKDQQIVYLKGYENQA
                     YGYGIIDGEDRIFKIDSDGTLAWKKALQEDVQGFSVFENKLVTWKAQTLSMFTLKGND
                     FHTVETDSLIQQVIAIGGHRLAILTSRNVIVYKY"
     gene            complement(3949..>5340)
                     /locus_tag="PKJ65_01940"
     CDS             complement(3949..>5340)
                     /locus_tag="PKJ65_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40633.1"
                     /translation="KGVLHEKKYTEYSRIIVALSSIGKDARDVAGYDLTYALGDYDKT
                     IWQGLNGPIWALIALDTKDYPIPENKEAKTQATRQKYIDRILECQLKDGGWNLFGGSS
                     IAAAQDEISDPDITGMALQALAKYQDQPKVAKATKEALACMSGKQGSSGSFDSWGTTN
                     SESCVQMLVALCELGIPLDDSRFAKNGVTLLDNLKTYQLENGSFLHTYSGSGSNQMAS
                     EQGLYGVVAALRAIEGKNSLYRMSDAITVEGSIDITVKKGEGLEGKHKDVLPMPITAP
                     GTTFEDITFSPNIAAIEALASRGIINGRSKKSFAPDADMTRAEFATIITRGLGLVEED
                     TKVFSDVSSDKWYAGYIGTANKYGIVNGIGEGKFNPEGTITRQEAATMVARAAALCGL
                     DTKMSNAEIRDVLSQFEDYVTVADWAMESMAFCYKKDILSDSALKVEPKVQIQRSEIA
                     QMLFNMLTVANLL"
BASE COUNT         1494 a         1202 c          896 g         1748 t
ORIGIN      
        1 aaatccattt ttttgagagt ttcttttata ctggaattta actttgcact caagccatat
       61 ctatcttatt gagatactct ttattcttaa tattatcctg tatataggtc accaaagact
      121 taaccatccg gtcatcatca atttccgaaa agattatatc tactaccgac tgagcatagt
      181 caggtttgcc ctgtgagata tattgataca gaatttcggt cattctgggg gctatatacc
      241 ctatagtatc ctgacgaaca gggatggatt taaggataat aaatgcataa tcggtttttt
      301 gtgtctccag gcgtaaatcg gtctgctcca acttgtaaaa catgtcttcc atatcgtccg
      361 aaaggaaaga aatacacacc gccgcatatt ccggacggga ataaattgat tccagttttc
      421 ttgaccattc cttaattgcg tctttaacgc cgggctccat gcgtataaga agttttatca
      481 atttatcatt taattcataa gctctatcca tatcatcctt gtaatacaga agtcttatcg
      541 ccagatctac cgaatactcc gcaagtttgg gtaccgtatt gctgtttata tccaccgata
      601 agatgacatc ggcagcctta tagtattctt ctctataaag caacacatat acaatgctta
      661 agagatattc ctgagcttta tcatagtttc tggaatcatc tatttccaca agcttgaact
      721 tattatgggc atcgtaatac aattcggcaa gatacaactc ccttgcttga ttataggctg
      781 ttcttgatgc ctgtattctt tcaacttcgc tttttgcatc ctccagatta tacagcggca
      841 tttccagcag cgagaaaaga ttcagtttct tttccgcggt ttcgtatgag gtcttgtcct
      901 gcttgaatga tgcaactatg ttttcaatat attgatagac atactctgat gtattcatcg
      961 catgtacata attcttttca attttctcat actgtttgtt ggcttttctg taatcacctt
     1021 gcattaaagt gtcaaccatg ttaatccacg gctggttgtc acaacctgtc agtataaagg
     1081 ataatataaa aaggcaaatt ataagcaggc atactttttt catagaaaaa gtatatccca
     1141 tattgccttt tcattcaata taatgcgtta aacagctcct tgtaaaactt cttttttgta
     1201 atcggatatg tattctatga ggcagttggc tattatctcg tgtccttttt cattgggatg
     1261 aatgccgtct ctgcataaca gctccatcag attccttgca tttaaaaacg cctttctgat
     1321 gtccacaagc cgcacattca gttgttttga tatgctttct atagtaagag aataaagctc
     1381 ctgaaatctg tatatcttga atacatcctt aagccacttc agtatggctt cttttgatgt
     1441 tccgtctttt gtaatccaat ccagatatct gacgggatct atcgggtgca ggttggtaag
     1501 aacaggttcg ataccttttt ctctgaacat ggtaaccatc tgccttattg tcttatcaaa
     1561 cactttaata ggggtattgg ggtcatggtt aatatccggg tttgatgcta cttctttcca
     1621 gttaaaatcg cagtcattac ctcccaattc aataagtgca aaatccggtt taatatcatt
     1681 ttcttccaga ttcttcttca ttctttccaa agccttgggt gcggtcatac cgtatttgga
     1741 attattgaca tatgtgatat taaagttttt agcggtttcc ggtatgcaac tgttctgaag
     1801 aaaaacatac ttaccggcat tgtcatcata cacaacgcct ctcaaaaccg aatctcccca
     1861 tattgatata ttggtcataa caaaatcctc cccgtattac attaaactat atacgcggaa
     1921 gatgaactta atatgacttt ttaagcttat taagttaaat caatattaat aatgtataag
     1981 aaaaacctac ttcttaaaca actccagcag aagattgttg ttatacagca cacttgccag
     2041 tttgcttttc tgaattgttt catgaaccac actcattatt tccgctgtct ggaaaccgga
     2101 tataatcagg aatataatgt aaatcaacaa tacgcccgat accgctccga taatagctcc
     2161 tactattgca tccacctgct tgataaccgg cagttccgat atcttcttta ccaatcctct
     2221 gagaagtttg aatattatca acaacagaac gaataatatg atacctatga ttattgctat
     2281 tataaggtcg gttatgttag caaccaatcc gcccatagct tccgcttttg tggtggtttg
     2341 agaaaaatct attctctcta taagccaaat ctttatatca tcgggaaacg gcaaagaatt
     2401 gacggtagcg ataatcgctt cttctaccga atccagattg ggtaccaatg cttccgccat
     2461 atgctcaatc ttttcatgta tgttgttata gaaactggtt cccaccaata aatctctgat
     2521 gggctgagcg aatttaaagg agagaaaaat acttaagatg gtagtcagca atccatatgc
     2581 cgacatgatg attcccctta ttcctcccaa aatcatggaa acaattatta ttaatattaa
     2641 gatatagtct gctacattca tcacttatct ccttctctat atattctccc acacttcata
     2701 taaatcatca atctacagtt atctaatact tataaacaat cacattcctt gacgttaata
     2761 tggcaagtct gtgaccgcct atcgctatta cctgttgtat aagtgaatcg gtttccactg
     2821 tatggaaatc atttccctta agcgtaaaca tgctcaaggt ctgtgctttc caggtcacca
     2881 gtttattctc gaaaaccgaa aatccctgaa catcctcttg taatgctttt ttccatgcca
     2941 atgtgccgtc ggaatctatc ttaaatatcc tgtcttcacc gtctattata ccatatccgt
     3001 atgcttggtt ttcatatcct tttaaataaa caatctgttg atctttaaaa accgaggtct
     3061 tttccattac tttatctatc agcaaaacat cggtatcatt ggccacaaga aatcttccgt
     3121 cttgtaaaca taccgttttg ggcaggaacc cgtccaatgt gatacctgct atcggagtgg
     3181 gtttccctat ttcaaatact tctatatgtg tttttgacat atcatcgggg acacaggaaa
     3241 caaccgcaaa ctgctcctta tcatcaaaca tgtcaagata aacgggatat ctcccggaat
     3301 attgtatatc gttcttgtat ccgtcgggta ttatgaggtt tgtctgatat gtcgaacagt
     3361 ataccgctcc gaggtatcct tgtaagcctt cgcaaagtaa aaacatatat tcatcaaatg
     3421 cgaatacata tgttatggcg gtttttgcag tatattcatc taccttctta ccttcacaaa
     3481 aaacggtgta cgatgtggca cctttatcat ataccactac atgcgtatcg gaataggcaa
     3541 tctttgtatc ggtataggaa agaagataag agcgtaaaaa ctctccgttg cttttgcaaa
     3601 cagataagcc gctgtcggta tagatatata tataaccgtt ttccgatgca acaacacttc
     3661 ccgttgctat aggaattgtg tattgtacgc cggcatcttt tgctactaca tcggccttaa
     3721 agaacctgag tatctccgtt ctgaaaaata tgaaaccgat tgctaccaat accgcaagca
     3781 gcacgacaat tataatccag taagcttttt tctttcgctt tttagccaat taaagatatc
     3841 tcctatcact tttatcaaac atattctacc acaatacata tgtccataaa agtgatgttt
     3901 cggtaagata gtgtaaactt gcatgtataa agcacacctt aacaactatc atagcaaatt
     3961 cgcaacagtt aacatattaa acagcatctg cgctatttcg cttctttgta tctgtacttt
     4021 aggctccacc ttaagtgcgg agtcgctgag tatatctttt ttgtaacaaa aagccattga
     4081 ttccattgcc caatccgcca ccgttacata gtcttcaaac tgagataaaa catcccttat
     4141 ctcggcattg ctcatctttg tatccagtcc gcataatgca gctgcacgag caaccattgt
     4201 agcggcttcc tgccttgtaa tagtgccttc gggattgaat tttccctctc cgatgccgtt
     4261 tactatcccg tacttgttgg ctgttcctat ataacccgca taccacttat ccgaagatac
     4321 atcgctgaac actttggtat cctcttcaac aagacctaac ccgcgggtta ttatggtagc
     4381 aaactccgct ctggtcatat cggcatcggg tgcaaagctc ttcttgcttc tcccgtttat
     4441 aatacccctc gaagcaagcg cctctatcgc cgctatattg ggactgaatg taatatcttc
     4501 aaaagtggtg ccgggtgcgg ttatgggcat aggcaacaca tctttatgct tcccctcgag
     4561 accttcacct tttttaacgg taatatcaat agaaccttct acagttatag catcactcat
     4621 tctgtagaga ctgtttttcc cctcaatcgc tctaagagcc gcaactacac cgtaaagccc
     4681 ctgttccgaa gccatctgat tgctgccgga gccggaatat gtgtgaagaa aactgccgtt
     4741 ctccaactga taagtcttta aattatccaa aagagtaaca ccgtttttcg caaaacgtga
     4801 atcgtccaaa ggtattccga gttcacacag tgcgacaagc atttgtacgc agctttcgga
     4861 atttgttgtt ccccaactgt caaaactgcc gctactaccc tgcttaccgc tcatacatgc
     4921 taaagcctct tttgtcgctt tagccacttt aggttggtcc tgatattttg caagagcctg
     4981 aagtgccatt ccggtaatgt ccggatcgga aatttcgtcc tgagcggcag ctatggaact
     5041 tcctccgaac aaattccagc ctccgtcttt tagctgacac tctaaaatgc ggtctatata
     5101 cttctgtctt gtcgcctgag ttttggcttc tttgttttcc ggaatgggat aatcctttgt
     5161 atccaatgca attaaagccc atattggtcc gttaagtccc tgccatatag ttttatcgta
     5221 atcacctaaa gcataagtaa ggtcatagcc tgcaacatca cgggcatctt taccgattga
     5281 agaaagagct acaattatac ggctgtattc cgtatatttc ttttcatgca acactccctt
//
LOCUS       DAOPWC010000052         5321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15467_length_5321_cov_3.513483, whole genome shotgun sequence.
ACCESSION   DAOPWC010000052 DAOPWC010000000
VERSION     DAOPWC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15467_length_5321_cov_3.513483"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKJ65_01945"
     CDS             <1..797
                     /locus_tag="PKJ65_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ40634.1"
                     /translation="FILAALVLGIFLFLRDKKNNVKFTILGNGTEKEKKNLLIAGIAC
                     GLIQTVANFFQMSGLKHTTVGKAGFITVLYIFIVPIAGLLLFKKRVTLAVWISVLIAL
                     AGSYLLFIQEGFSINKGDLLVLVSAFLFAAHIMVIDHFTKITDSIRMVFLQYAICGIL
                     SLIAMFVFENPKWDTIVNASIHIAYAGILSSGVASSLQFVSQKNISAPVASIIMSMES
                     VFAALAGWIFLQEILSLKEIAGCLLIFFGIILAQIPFRSLKRTGKS"
     gene            858..1220
                     /locus_tag="PKJ65_01950"
     CDS             858..1220
                     /locus_tag="PKJ65_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNZ40635.1"
                     /translation="MNIQESAEMYLETILILSKKSDTVRSIDIVNYTGYTKPSISRAV
                     NLLKKNGYLNIDEKGYITLTASGESIAKNIYERHCVLTDVLLKMGIDDKTAKQDACKI
                     EHVISEETFNKIKEYLNK"
     gene            1235..2164
                     /locus_tag="PKJ65_01955"
     CDS             1235..2164
                     /locus_tag="PKJ65_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40636.1"
                     /translation="MTPKQRAITALNRGIPDEIPTFELEFQLSYELVGKEFLRQEQLE
                     NMSKKEKDIALHENAELMVEVYEKLDYSVIPIHYLWLDEAIKTAKIINRMTNDRYLLI
                     RHGDGTFSIPDGNEMYEFSYRIADDPKGLLEEAEKMAANVIERNKRAIDAGIGSIMLC
                     SDYCYNSGPFLSPYHFSVFITPFLAKIIKGIRDAGGYAIKHTDGNIMPILDQLIEANP
                     HAIHSLDPMAGVDIAEVKRIAGDKVCLIGNVNCALLQTGTDEEVIESAKYCLTHAKPG
                     GGYIYSTSNVPFKGLPLERYLLILDVWKQYKKY"
     gene            complement(2223..2397)
                     /gene="ssrS"
                     /locus_tag="PKJ65_01960"
     ncRNA           complement(2223..2397)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKJ65_01960"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2466..3344)
                     /locus_tag="PKJ65_01965"
     CDS             complement(2466..3344)
                     /locus_tag="PKJ65_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNZ40637.1"
                     /translation="MKIKHVISDDCIILENLNPDDFNIKDTFECGQCFRWEKTDRQNG
                     YLGIAHGKKLIVQQNEDYITLHCNEKDFLHIWQYYFDLDTDYDEIKERLRKDDIMNKA
                     ICSAPGIRILNQELFETAVSFITSSNSNIKRITRNIKDLCALLGQPIGDGYYAFPTVE
                     ALSNADICTINQCRAGFRCDYIHSSANMIKNMSDSFDKTRYINLGYEETKKELMRLKG
                     VGNKVADCIILFAGISSSAFPTDVWVKRIMQNLYIKKEVTLDYISKYGRDKFGDLAGY
                     AQQYLFHYARNIAVHK"
     gene            complement(3334..>5321)
                     /locus_tag="PKJ65_01970"
     CDS             complement(3334..>5321)
                     /locus_tag="PKJ65_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNZ40638.1"
                     /translation="RQVHLDFHTGEQVTGIGANFNPEEFAKTLKDAHVNSVTCFARCH
                     HGMIYYDSKINKERIHPHLVEKDLLKKQIEACHKYDIKVPVYTTVQWDLYTAKKHPEW
                     LVRTPEGSVDGNYFNKDGFYQRICVNTPYMDFLKKHVTELLTELDCDGVFLDIVGDLD
                     CSCVYCMESMEKGGFNPLDENDRRAHYRQVMTNFKKEMSRLIWSLKPGILIFYNGGHV
                     SYKDKLPSKDTYSHYEIESLPSGGWGYMHFPVTCRYARNLGKDYLAHTGKFHTSWGDF
                     HSFKNIEALEYEVFRMLALNSKCLIGDQLEPDGALSKPVYDLIGKVYARVEQKEPWCK
                     NADHVAEIGVFVPTDEKTRVSASQAAIESLLDQLAYQFDFIDPYMDFSRYRMLIIPER
                     ITIDEQTDKKLCGYMKNGGKVLSVFDAIFSGSGVKVIKETLDDYGQPVKGRFVARNRA
                     ADYVIPTDLIGKELYKTEYVMYAKGMETVATDGKALVKAYEPIFNRSFRHFSSHAQAP
                     SSHIEAYDAVVINKSKNAIYFSHPIFEIYNKKGPKWIKSMLHDAIKLLLKDNLISHDG
                     PSTLFTGMNVQKDKKRYILHLLHYIPERRCTDFDLIEDVIPLYRIKINLNLPKTIKSI
                     TVVPENKPVKFTGKGGKAKFTLDKLEGHCMLCIEYED"
BASE COUNT         1563 a         1114 c         1021 g         1623 t
ORIGIN      
        1 gtttcattct tgccgcactg gtactgggaa tatttctgtt tttaagggat aagaagaata
       61 acgtaaagtt tactattctc ggaaacggta cggagaagga aaaaaagaat cttcttattg
      121 ccggtatcgc ttgcggattg atacagaccg ttgccaactt ctttcagatg tcgggattaa
      181 aacatacaac cgtgggtaaa gcgggcttta taacggtact gtatatattc attgttccga
      241 ttgccggttt attattgttc aaaaagaggg ttaccttagc ggtatggata agcgtactca
      301 tagcattggc aggctcttat ctgctcttta ttcaggaagg attcagcata aacaaagggg
      361 atcttctggt actggtaagt gcattcttat ttgccgcaca cataatggta atagaccatt
      421 ttacaaagat aaccgacagc atcagaatgg tctttctgca atatgcaata tgcggcatac
      481 tgtcgcttat agcaatgttc gtgtttgaaa atcctaaatg ggatacaata gtcaatgcct
      541 ctattcatat agcatatgcc ggtatattat catcgggtgt tgcctccagc ctgcagtttg
      601 taagccagaa gaatatttcc gctcccgtag cttccataat aatgagcatg gaatcggtat
      661 ttgccgcttt agcaggttgg atatttttac aggagattct ttcattaaaa gaaatagcgg
      721 gctgtctgtt gattttcttc ggaataatac ttgctcagat tcccttcagg tcactgaaaa
      781 gaacaggtaa gagctgaact gtttttttat atttctttat gatacaattt aataaaatcc
      841 tgacgaaagg ctgagtcatg aacatacagg aatccgctga aatgtatctg gaaactatac
      901 tgattctgtc gaaaaaatcc gacacggtac gttccataga tatagtaaac tacaccgggt
      961 atacaaaacc cagcataagc cgggccgtca acttgttgaa aaaaaacggc tatctgaata
     1021 tagatgaaaa gggatatatc actttaaccg cttcgggaga aagtatagca aaaaacatct
     1081 atgaacggca ctgtgtgctg accgatgttc ttcttaaaat gggtatagat gataagacgg
     1141 caaaacagga tgcttgcaaa atagagcatg ttataagtga ggaaaccttt aataaaatta
     1201 aagagtattt aaataagtag tatcgggagg aattatgaca cctaaacaaa gagcaataac
     1261 cgcgcttaac aggggtattc ccgatgaaat acctacattt gaactggaat tccagctttc
     1321 ttatgaactg gtaggtaaag agtttcttcg gcaggaacag ctggagaata tgagcaagaa
     1381 ggaaaaagac attgcactgc atgaaaatgc cgagctgatg gtggaagtat atgaaaaact
     1441 tgactattcg gtaattccta ttcactatct ttggctggat gaggccataa agaccgcaaa
     1501 gattataaac aggatgacaa acgacagata ccttctcata agacacggag acggaacatt
     1561 cagtatcccg gacggcaatg aaatgtacga attttcttac agaattgccg atgatcccaa
     1621 aggtcttctt gaagaggcgg aaaagatggc tgcaaatgtg atagagagga ataaaagggc
     1681 cattgatgcg ggaataggca gtataatgct ctgttccgat tactgctata acagcgggcc
     1741 tttcttatca ccttaccatt tctccgtatt tataaccccc tttttggcaa agataataaa
     1801 aggtataaga gatgcgggcg gatatgcaat caagcatacc gacggcaaca taatgcccat
     1861 actggaccag cttatagaag ccaatccaca tgccattcac tcgcttgacc ccatggcggg
     1921 agtggatatt gccgaagtaa aaagaattgc cggagacaaa gtgtgtctta taggtaatgt
     1981 caactgtgcc ttgcttcaga ccggaaccga tgaagaagtt atagaaagtg caaaatactg
     2041 tctcacccac gccaaaccgg gcgggggtta catatattcc accagtaatg tacctttcaa
     2101 aggactgccg cttgaaagat acctgcttat tctggatgtc tggaaacaat ataaaaaata
     2161 ttaaatgaga caaaaacctt cattacttct attatctaat tcatataaaa aagagaaata
     2221 taaaaattcc caaaataccg gccctttttt ttgacgccta gcgtccgcca ggtgggttcc
     2281 gtgatgaatg tttctgtctt ccgctgatat acggcctgac atccgcacac atactactaa
     2341 atcccgtcaa attactgtag gttcaaatac aagggttatc gtgtatctca ggaatctact
     2401 acctgaatta tacaatatta ccggtttttc atcaatattt aatattcctc taagatatga
     2461 tataattatt tatgaacggc aatatttcgt gcatagtgga aaagatactg ctgcgcatat
     2521 cctgccaaat caccgaattt gtcccgtccg tatttcgata tgtagtccag agtgacttct
     2581 ttttttatgt ataagttctg cattattctt ttaacccaca catcggtagg aaaagcggaa
     2641 gaagaaatgc ccgcaaataa aataatacag tcggcaacct tattccccac ccctttaagt
     2701 ctcataagct ccttctttgt ctcttcataa cccaaattaa tatatcttgt cttatcaaaa
     2761 gaatcggaca tattcttaat catgtttgca gatgagtgta tatagtcaca acggaatccc
     2821 gctctgcatt gatttattgt gcatatatcc gcattactta aagcttccac ggtcggaaaa
     2881 gcataataac cgtcgcctat cggctgcccc aacagtgcgc acaaatcttt tatattcctc
     2941 gttatccttt taatattact gttagatgat gttataaaac tcacagcggt ctcaaacagc
     3001 tcctgattga gtatccttat accgggagcg gagcatatgg ccttgttcat gatatcatct
     3061 tttctaagtc gttcctttat ctcatcgtaa tcggtatcca aatcaaagta atactgccag
     3121 atatgtaaaa agtccttttc attgcaatgt aacgttatat aatcctcatt ttgctgcacg
     3181 ataagcttct tgccgtgagc tatacccaga taaccgtttt gtctgtctgt tttttcccat
     3241 cggaaacact gaccgcactc aaacgtatcc ttaatgttaa agtcgtcggg atttaagttt
     3301 tccagtatga tacagtcatc acttatgaca tgcttaatct tcatattcta tgcatagcat
     3361 gcaatggcct tccagtttat ccagtgtaaa cttagctttg ccgcctttcc ccgtaaactt
     3421 aacaggcttg ttttccggca caacggttat actcttaatt gtcttcggaa ggtttaagtt
     3481 tatctttatc ctgtataaag gtatcacatc ttctatgaga tcaaagtcgg tacatcgtct
     3541 ttcgggaatg taatggagca ggtgcagaat ataccttttc ttatctttct gtacattcat
     3601 tcccgtaaag agcgttgacg gtccgtcatg agatataagg ttatctttta ataaaagctt
     3661 tattgcatca tgaagcatgc tctttatcca tttgggcccc tttttgttat aaatctcaaa
     3721 tatgggatgg ctgaaatata tcgcattttt actcttgttt atcaccaccg catcgtatgc
     3781 ttcaatgtga gaagaagggg cttgtgcatg tgaagagaaa tgcctgaatg atctgttgaa
     3841 tatgggctcg taagctttga caagagcctt tccgtcagtt gcaacggttt ccatgccttt
     3901 tgcatacatt acatactccg ttttatacaa ctccttacca atcagatcgg taggaataac
     3961 ataatcggcg gctcggtttc ttgcaacaaa tcttccttta acaggctgac cgtaatcgtc
     4021 taatgtttct ttaatgactt taactcccga gccggagaat atggcatcaa ataccgataa
     4081 gactttgccc ccgtttttca tatacccgca aagcttctta tccgtttgtt catctattgt
     4141 tattctctcg ggaatgataa gcattctgta tctgctaaaa tccatatacg gatctataaa
     4201 atcaaactga taagccaact ggtcaagaag actctctata gcggcctgag aggcggaaac
     4261 tctggttttt tcatccgtag gcacaaacac ccctatctcc gccacatggt cggcattttt
     4321 gcaccacggt tccttctgtt ccacccgtgc ataaacctta cctattaagt catatacagg
     4381 ttttgacaaa gctccgtcgg gttccaactg atctcctatc agacacttgg aattaagagc
     4441 aagcattctg aacacttcat attctaaagc ctcaatattc ttgaatgagt gaaagtcgcc
     4501 ccagcttgta tggaatttgc cggtgtgtgc aagatagtct ttacctaaat ttcgcgcata
     4561 tctgcaggtt accggaaagt gcatatagcc ccatcccccg ctcggaagac tttctatctc
     4621 ataatgtgag tatgtatctt ttgacggcag tttatcttta taggaaacat gaccgccgtt
     4681 atagaatatc aatataccgg gttttaatga ccaaataagt cgggacattt cctttttgaa
     4741 atttgtcata acctgtctgt aatgtgctct tctgtcgttt tcgtcaagag ggttaaaacc
     4801 gcctttttcc atactctcca tacagtagac gcaggagcag tccagatctc ccactatatc
     4861 caaaaacact ccgtcacagt ccagttccgt caacagttcc gtcacatgct tctttaaaaa
     4921 atccatatac ggtgtgttga cgcaaatcct ctgatagaaa ccgtccttgt tgaagtaatt
     4981 gccgtcgaca gatccttcag gcgttcttac cagccattcg ggatgctttt tggcggtgta
     5041 taaatcccat tgtacggtag tgtacacggg aacctttata tcatacttat gacaggcttc
     5101 aatctgtttt tttaataaat ccttctctac cagatgagga tgtattcttt ctttatttat
     5161 cttggaatcg taatatatca ttccgtgatg acatcttgca aaacatgtaa cggaattcac
     5221 atgagcgtct tttaatgttt ttgcgaactc ttccggatta aaatttgcac ctataccggt
     5281 aacctgttcg cccgtatgga aatccaaatg tacctgtctg a
//
LOCUS       DAOPWC010000053         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15562_length_5297_cov_3.480923, whole genome shotgun sequence.
ACCESSION   DAOPWC010000053 DAOPWC010000000
VERSION     DAOPWC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15562_length_5297_cov_3.480923"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKJ65_01975"
     CDS             <1..668
                     /locus_tag="PKJ65_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ40639.1"
                     /translation="DIPLHLSETVVKIHGKGRITGVTTAKVDSNMKPIKGTEQYVECD
                     TLLLSVGLIPENELSEQMGVGMYQVTGGPFVDQDLETDTEGVFACGNVLHVHDLVDYV
                     TMEGQRAAMSAKAYLDKTLTEKKSAQKIAVVAGNGLRYVLPQHIYLKDTEEKIKIFFR
                     SRARFREVYVRASVGDNVLTRRKLLRLIPSEMESVDIKMRDLLNTGSETNGFGDLVIE
                     VEQ"
     gene            665..1048
                     /locus_tag="PKJ65_01980"
     CDS             665..1048
                     /locus_tag="PKJ65_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HNZ40640.1"
                     /translation="MTKEKIMKKDIVCIVCPRGCTIQVNYDKNGIISMEGSSCKRGDE
                     YARAEILSPTRMITSSVKIDNAKYTVCPVKTGAPVSKSKIFDVMEEIYKVRIKAPVQQ
                     GDVIIKDVCGTGADIIATQTFETES"
     gene            1051..2442
                     /gene="murC"
                     /locus_tag="PKJ65_01985"
     CDS             1051..2442
                     /gene="murC"
                     /locus_tag="PKJ65_01985"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNZ40641.1"
                     /translation="MKAGFLQGIKHVHFIGINGTSMSGLAILLSEKGIKITGSDIEHT
                     ATMDKLLSMGIYVYIPHDASKVGKPDLVVYTAAVHPDNCEYAYAVSKEIPVMDRAVLL
                     GKIMEEFKYSIAISGAHGKTTTTTLAAYLLEKMGYDATIHNGGEAEFLNGNVKSGNSD
                     YFLTEACEFNGSFLKLKPYIGVILNIDFDHVDWFKTFDNMKRSFLEFADKIDKDGFLV
                     ACYDDIPAREVAKKASRNVVYYGLNNLSLDYTAMNMTIDTQGKPSYDLYVKGYFICHV
                     QLGAEGQHNALNSLAVIAVMGLLGADLTEVCTHLSFVHGAKRRFDLIGEADGIKVYSD
                     YAHHPTEIAVTLETASRMKHNKLYAAFEAHTFSRVRVLFEEFVQSFALADIAIIADIY
                     NDRESPDSEVSGKRLAQAINKNVTKAIYLPSYQAIEDYLFEHAKSGDIVMVLGSKYLE
                     TICKPLLKRLEKR"
     gene            2575..3270
                     /locus_tag="PKJ65_01990"
     CDS             2575..3270
                     /locus_tag="PKJ65_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNZ40642.1"
                     /translation="MKQVLRQEKKYIISKTDYIKYSAAFNTYLAQDENNGEHGYMVRS
                     LYFDTLWDNDFVEKEDGVEIRRKIRLRIYKPTDQFAGLEMKQKQGSQQLKRSLKMTRE
                     DAILLTKGIYTPLLGYKDPFAKELYAVMNMRFYRPKAIVEYNRKAFIAKENSIRITFD
                     HNIRATESCFDIFSENLNTYPVFSQDGVILEVKYNGFLLSYIKDMLDRVEQSELSVSK
                     YALSRTICLNYVF"
     gene            3285..3962
                     /locus_tag="PKJ65_01995"
     CDS             3285..3962
                     /locus_tag="PKJ65_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNZ40643.1"
                     /translation="MTKYLLELLEQGKTLTVQEILSHLAVAAVVGVVIFLSYMYTHTG
                     TIYSRKFNVSLLMLTLLTTMVMTVIGNNIALSLGMVGALSIVRYRTAIKDSRDTAYIF
                     WAIIAGICCGAGDYLVAAIGSTVVFLLLLAFGKVRNDNRMLVIVKGARNSERQIEAII
                     FNYYSAKANLRVKNTTELTVEYIYEVSKKVMRKAAANEKSITERLYELQHIEYVNIVE
                     QNDEINN"
     gene            3974..>5297
                     /locus_tag="PKJ65_02000"
     CDS             3974..>5297
                     /locus_tag="PKJ65_02000"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNZ40644.1"
                     /translation="MRRKLIISSIALICLIAVAVSLSYLNYGNRRHHQHIADVKHVKC
                     NHTDGRFCTHLPLVHINTDGQDIPVLADNSMVIDADLSIFDKGDSNNHLDDQADLHSL
                     ITIRARGNTSLWFDKKSYLIRLIKEDRTENAKKVMGMEAHDKWILNGPILDKTLIRNY
                     ISMNIAGEIMSNSPDVRFCEVFVNDEYMGVYVMMEAISRGKGRVDISRYREDRPFTSY
                     IVRLDRGTVPEEEIDPFTGYTYNMNSICNIVYPARKNINEQIKTYIENDVSAFEKALY
                     SYDYADKELGYRKHIDVMSFVDYLIINEFTQNYDAEIYSTYLYRDTKGKIGIGPVWDF
                     NNAYDNYMETPMSATGFSFQNKVWYIMLMKDDYFTGLVIKRYKVLRKTVLSDEYLINY
                     IDKTVRYLGPAIDRNFEKWGYTFLIDKGLLEPAERNLKSYDAALNQLKN"
BASE COUNT         1736 a          929 c         1247 g         1385 t
ORIGIN      
        1 acgatattcc tttacacctg tccgaaacgg tggtcaagat acacggaaaa ggtaggataa
       61 cgggagtcac caccgccaag gtggacagca acatgaaacc tataaaaggc accgaacaat
      121 atgtggaatg cgatacgctg ctgttgtcgg tcggccttat accggaaaac gaattaagtg
      181 agcagatggg tgtgggcatg tatcaggtta cggggggtcc ttttgtggat caggacctgg
      241 aaaccgatac cgaaggggta tttgcatgcg gaaatgtgct gcatgtgcat gatcttgtgg
      301 attatgtaac gatggaaggt caaagagcgg ctatgagtgc aaaagcctat ctggataaaa
      361 ctttgacaga aaaaaagtct gcacaaaaaa tagcggtggt tgccggaaac ggattgagat
      421 atgtgttgcc gcagcatata tatttaaagg atacggagga aaagataaag atatttttcc
      481 gttcaagggc gcgattccgc gaagtatatg taagggcttc tgtcggtgat aatgtgctta
      541 cccgaagaaa gttgctgcgt ctcattcctt cggaaatgga atcggtggat ataaagatga
      601 gggatttgtt gaatacgggc agtgaaacaa acggtttcgg cgatttggtc atagaggtgg
      661 agcagtgaca aaagagaaga taatgaaaaa agatatagtc tgtatagtat gccccagggg
      721 atgtacgata caagtgaatt atgacaaaaa cggcattata agcatggaag gcagcagttg
      781 caaaaggggc gatgaatatg ccagagccga gatactttcc cctacaagaa tgattacttc
      841 ctccgttaag atagataacg caaaatatac ggtctgtccc gtaaaaaccg gcgctcccgt
      901 aagtaagagt aagattttcg atgtaatgga agagatatat aaagtaagaa taaaggctcc
      961 cgttcagcag ggagatgtga taattaagga tgtgtgcggt acgggtgccg atataattgc
     1021 cacacagact tttgagacgg agagctaagc atgaaagccg gatttttgca gggaatcaaa
     1081 catgtgcatt ttataggtat aaacggtact tccatgtccg gtcttgccat attattgtcg
     1141 gaaaaaggca taaagataac cggctccgac atagagcata ccgctacaat ggataaactg
     1201 ttatccatgg gtatttatgt atatatacca catgatgcct ccaaagtggg aaaacccgat
     1261 cttgtggtat ataccgcggc ggtgcatccg gataattgcg aatatgcata tgcagtgtct
     1321 aaagagatac ctgtaatgga cagagcggta cttttaggta agataatgga agagttcaag
     1381 tactcgatag ccatatcggg agctcacgga aaaaccacaa ccaccactct tgcagcatac
     1441 ttattggaga agatgggata tgatgccacc atacacaacg gcggggaagc ggagtttctg
     1501 aacggtaacg taaaaagcgg caactccgat tactttctga ccgaagcctg cgaattcaac
     1561 ggcagtttct taaagcttaa accgtatatc ggagttatac tgaatattga ctttgaccat
     1621 gtggactggt tcaaaacctt cgacaatatg aagcggtctt ttttggaatt tgccgacaaa
     1681 atagataaag acggttttct ggtagcctgt tacgatgata ttcctgcaag ggaagtagca
     1741 aaaaaagctt cccgtaatgt cgtatactac ggtctgaata atttatcttt agactatacc
     1801 gctatgaata tgacaataga tacacaaggt aagccgtcat atgacctgta tgtcaaagga
     1861 tattttatct gtcatgtgca attgggagcc gaagggcaac acaatgcact gaactcactg
     1921 gccgtcattg cggttatggg gttattaggg gcggatctta ccgaagtgtg tactcatctg
     1981 tcatttgtac acggagccaa aagaagattt gatcttatag gagaagccga cggaataaag
     2041 gtgtattccg attacgctca tcatcccacc gaaatagcgg taacgctgga aacggcaagc
     2101 aggatgaaac ataataaact ttatgcggct tttgaagctc ataccttttc cagagtaagg
     2161 gtgttgtttg aggaatttgt gcaaagcttt gcccttgcgg atatcgctat aatcgccgac
     2221 atatacaatg acagggaaag tcccgacagt gaagtatcgg gaaaaaggct tgcccaagca
     2281 ataaacaaga atgttacaaa agcgatatat ctgccgtcat atcaggcgat agaggattat
     2341 cttttcgagc atgcgaaaag cggtgatata gtcatggtgc tgggctccaa atatttggaa
     2401 acaatatgta aacctttgtt gaaaagactg gaaaaaaggt aataagaata gtattttgag
     2461 ttaaatattt atttaagtta aattaaatca attaaaacag ctttatggaa gtaaagcctg
     2521 ttgttatttt attccttgaa taatataatc taaacgagag aaaaagagac gaaaatgaag
     2581 caggttttgc gacaggaaaa aaagtatatc atatcaaaga ccgattacat aaaatattcg
     2641 gccgctttta atacctacct tgctcaggac gagaacaatg gagagcacgg ctatatggtg
     2701 cgttctttat attttgatac tttatgggat aatgattttg tcgaaaaaga agacggcgtg
     2761 gaaataagaa gaaagatacg gttgagaata tataagccca ccgatcagtt tgccggtctt
     2821 gagatgaaac agaaacaggg ctcccagcag ctgaaaaggt ctttgaagat gacaagagaa
     2881 gatgccatac tgcttacaaa gggaatatat acacctttgc tcggctacaa agaccctttt
     2941 gccaaagaat tgtatgcggt catgaatatg cgtttttaca gacccaaagc catagtggaa
     3001 tataacagga aggcttttat tgccaaagag aacagtataa ggattacatt tgaccataat
     3061 attagggcga ccgagagttg ctttgacata ttttccgaaa atcttaacac atatccggtt
     3121 ttcagtcagg acggggtgat tttggaagta aaatacaacg gttttttact ttcctatatt
     3181 aaagacatgc tggacagagt ggagcaatcg gagttatcgg taagtaaata tgcactcagt
     3241 cgtactatat gccttaatta tgtattttaa aaaggagaag gattatgact aaatatttat
     3301 tggaattgct ggagcaggga aaaactctga ccgtacagga aatattgtcg catcttgccg
     3361 tagccgccgt tgtaggagtg gtaatatttc tgtcgtacat gtatacccac acgggtacta
     3421 tatacagcag gaaatttaat gtatctcttt tgatgctgac gctgcttacg acaatggtaa
     3481 tgacggtcat aggaaacaac atagccttgt cgttaggtat ggtaggtgca ttatccattg
     3541 taagataccg tacggcgatt aaggattcca gagatactgc atacattttc tgggcaatta
     3601 tcgcaggcat ctgttgcggt gccggagact atctggtagc cgctatagga agtaccgttg
     3661 tgttcctgct gcttcttgct ttcggtaagg taaggaatga caacaggatg ctggtaatcg
     3721 taaaaggagc acgaaactcc gagcgtcaga ttgaagccat tatattcaac tactattcgg
     3781 caaaagccaa tctcagagta aagaatacca ccgagcttac cgtggaatat atttacgagg
     3841 tatccaaaaa agtaatgaga aaagcggctg ccaacgaaaa aagcataacc gaaaggcttt
     3901 acgagttgca acacatcgag tatgtcaaca tcgtggaaca gaacgacgag ataaacaatt
     3961 aaaaggagag gtcttgcgta ggaagttaat tatcagttca atagccctta tatgccttat
     4021 tgccgtagcc gtttcgctgt cttacttaaa ttatggcaac agaagacatc atcagcatat
     4081 tgccgatgta aagcatgtga agtgtaatca tacggacggt cgtttctgta ctcatcttcc
     4141 tttagtgcat ataaatacgg acggacagga cataccggtt cttgccgata acagcatggt
     4201 tatcgatgcg gacctttcga tatttgacaa gggcgatagc aacaatcatc tggacgatca
     4261 ggcggatttg cacagtctca ttaccataag agcaagaggc aacacttcct tatggtttga
     4321 taaaaaatcc tatcttataa gacttataaa agaggaccgt acagagaacg ccaaaaaagt
     4381 aatgggtatg gaagcccatg ataagtggat tctcaacgga cctatactgg acaagacact
     4441 cataaggaat tatataagca tgaatatagc cggagagata atgagtaatt ctcccgatgt
     4501 ccgtttttgc gaggtatttg tcaatgatga atatatgggg gtatatgtca tgatggaagc
     4561 aatctcacgc ggcaaaggca gggtggatat ttccagatac agagaagaca gaccgtttac
     4621 aagttatatt gtccggctgg acagaggtac cgttcccgaa gaggagatag acccgtttac
     4681 cggatataca tacaacatga attccatatg taatattgtc tatcccgcaa gaaaaaacat
     4741 taacgagcag ataaagacat atattgaaaa cgatgtgtcg gcatttgaaa aagctttata
     4801 ctcctatgat tatgccgata aagaattagg ctaccgtaaa catatcgatg taatgtcttt
     4861 cgtggattat ttgattataa acgagtttac gcaaaattat gatgcggaaa tttactccac
     4921 atatctttac agagatacaa aagggaagat aggaataggt ccggtatggg attttaacaa
     4981 tgcttatgat aactatatgg aaacacctat gagcgctacc ggattttcct tccagaataa
     5041 agtatggtac ataatgctca tgaaagacga ctattttacc ggactggtaa ttaaaaggta
     5101 taaggttctg agaaagacgg tgctaagcga cgagtattta ataaactata tcgacaagac
     5161 cgtaagatat ctcggacctg ctattgacag aaattttgag aagtggggat atactttctt
     5221 aatagataaa ggattgctgg agcctgcaga aagaaatctc aaaagttacg atgcggcatt
     5281 gaatcagctt aagaact
//
LOCUS       DAOPWC010000054         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15717_length_5263_cov_3.760561, whole genome shotgun sequence.
ACCESSION   DAOPWC010000054 DAOPWC010000000
VERSION     DAOPWC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15717_length_5263_cov_3.760561"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PKJ65_02005"
     CDS             complement(<1..931)
                     /locus_tag="PKJ65_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40645.1"
                     /translation="MLGGKEDIVGHIDYMGIDINDKANLVKFSYFLSEYFADYNVLSQ
                     YEKRINKFNEPYQYMYVNNVDILPINLSEDSGFEKNIITVTGIDGNDNLQMSHVYGDA
                     LIEDYHKYSQYITDIIISTGVKGEDWSKYPLNVKIPCYGINFGGYGLEGFYVKYTEYY
                     QSHGMDSPWYHQAVTSIREIKSMKKNCDITFYTNYTEKDLVVIRKEDYVEPKDHLDSG
                     ITGYAIVPKMIWESVRNHPDIDYQIIRLFEQPKKDDNPSDRMRFGFKVIGYYETADES
                     DTVYVTYGGYNRKYVKEPFKNECIRSMIIETRSD"
     gene            complement(879..1691)
                     /locus_tag="PKJ65_02010"
     CDS             complement(879..1691)
                     /locus_tag="PKJ65_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40646.1"
                     /translation="MVKKKKTGLIITITVIVLAVVAALLFLFRNRLFCNIGHFNVTTF
                     NSDIVIKRSDAQEPLNMPYRYSKALLDKRLVFREEIERLNITTVRYEISKTGLALYNC
                     KEVLKNPESGETKKVIESIKYCKGITALSGLKADKADSKITIYRGYSADLLEQSLHNY
                     VIIPSTLSEHIDSQLSDNEKVLFLVNSGTSGLAYFTIIGEYETKHKHDTLYFSYSGLS
                     NVVLGGKEDIVDMTDNIFFPSQHHFVLFLFRIEQCGAGRERRYCRSYRLHGD"
     gene            complement(1773..3587)
                     /locus_tag="PKJ65_02015"
     CDS             complement(1773..3587)
                     /locus_tag="PKJ65_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40647.1"
                     /translation="MKKTNMIIAVALTVIITALLLASCDGLSKKEEGLIDATFSSDIV
                     IKRVDGKQPANIPYKYTKALLSDNIAYIDGVQAVNISSRRYNIKDNDISVRGDRDLFV
                     SSEEEKAAEVMGSIKYCKGITSLCTIVADKGKSKVEFYAGYSEEYLTSVDKAYVIIPS
                     SIGELINEEIPDSDKRIVLEKGAFGITYFTIIGEYEVKDRSKNKGEPDTLYLSYAGFG
                     NLINMGEEIDISFVDCLAIDADEQADLTKLTYCLTTYFADANTLSQYEGRINMFNENY
                     SYMYENSVNMKSLDLSPDTDCEKKIITVSRIDGKSDLDMSYVYADALVRDYNKYSQYI
                     TDINISTGLGGHDPYENAKLESIDKGGGFIQTNFILDNADGLNISFASSSTGFRDAVT
                     NMITQNRKDALADVGKGKAWKKHVQYLLHQPITSISEIERMKSNSEVTFYTNYTNRDL
                     VVQRKEDYIESTFKSDGDIKGYAIIPKTMHDAASNLPDLKTHILYLSEKNDAVESNQK
                     VLVGFKVIGYYETDDRYDVVYITYAGSNDKYTKERFRNDYINSVTIETKKDTDITPLL
                     EYLEQYFVPATDTSKCAGKKNALGRDYEYCYTIKSNAD"
     gene            complement(3651..>5263)
                     /locus_tag="PKJ65_02020"
     CDS             complement(3651..>5263)
                     /locus_tag="PKJ65_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40648.1"
                     /translation="VVTTFNSDIVIKRTDANESLDMPYRYTKALMDNMFLFRQEIVGI
                     NIASVKYSMSDNYIDWHTPEGVLTDTDRGKGKQVIDAVKYFKGISTLSSIVADKEDCK
                     ITIYEGYSEDLLIHDYQNFAIIPSSMSKYFNKDLPADEKVLNIRNMRYGSMLHFTIIG
                     EYKTEEEYDTLYVTYTGLSTLIRAGRTDILNHVDCLEIDVNEDKDLNKLMRFLSEYYA
                     DAQVLSQYTERNNIYNDPYQYMFVHSMSIEPIELKEDVIYEKNIITITRMDGKEDLEM
                     SHVYADAIIKGYNKYSQCITDLDISTGVKGINPADYPPGSEAFWNQPVYQLLLKYDTV
                     YEAKLKETLGDFPCYHQAVTSINEILRMKKDCKVTYYLNYMNSDLIVPRQKDLLGKIK
                     GYAIVPKPLHEATSDLPNFNNHIVEVYESRVYVGIGGVDPSQIDRSPHFRAQFKIIGY
                     YETTDPYDTVFVTYVGCNEKYKSAAFKNEHIESITMKTKGDVEISPLINFLKLYFAPS
                     ENAAEYAGSTNELGLAYEYSFTMKEIAE"
BASE COUNT         1438 a         1139 c          839 g         1847 t
ORIGIN      
        1 catcgcttct tgtttctata atcatagacc ggatgcactc gtttttgaaa ggctccttta
       61 catacttcct gttatacccg ccatatgtaa catacaccgt atcactttcg tcagcggttt
      121 cataatagcc tattacttta aatccgaatc tcattctgtc cgaagggttg tcgtcttttt
      181 tcggttgctc aaacaacctt ataatctggt agtctatatc gggatggttg cggaccgatt
      241 cccatatcat tttaggcaca attgcatatc ccgttatgcc gctgtccaag tgatccttgg
      301 gttccacata gtcctctttt ctgataacaa caaggtcctt ttcggtatag ttggtataaa
      361 atgttatatc acaatttttt ttcatacttt taatttctct tatacttgta acagcctgat
      421 ggtaccaagg actatccata ccgtgtgatt ggtagtattc cgtatatttc acataaaagc
      481 cttccaatcc gtaacctccg aaattaatac cgtaacaggg aatctttaca tttagcgggt
      541 acttagacca atcctctcct tttacccctg tgcttattat tatgtccgtt atatattgcg
      601 aatatttatg gtagtcttca attaatgcat caccgtatac atgcgacatc tgaagattat
      661 cgttaccgtc tattccggtt acggttatta tattcttttc aaaaccggaa tcttccgaaa
      721 gatttatcgg caggatatca acattattta catacatata ttgataaggt tcattgaatt
      781 tgtttattct tttttcatac tgtgaaagca cattatagtc tgcaaaatat tcgctcagga
      841 aatatgagaa ttttacaagg ttcgctttat cgtttatatc aatccccatg tagtcgatat
      901 gaccgacaat atcttctttc cctcccagca ccacattgct caatccggaa taggaaaagt
      961 acaaagtggt gctgggaggg aaagaagata ttgtcggtca tatcgacaat atcttctttc
     1021 cctcccagca ccacattgct caatccggaa taggaaaagt acaaagtgtc atgtttgtgc
     1081 ttggtctcat attcccctat tatggtgaaa tatgccagac cgcttgtgcc ggaatttaca
     1141 aggaacaata ctttttcatt atcggataat tgagaatcta tatgttcgct aagggtagag
     1201 ggaattatta catagttatg taaactctgt tccagaaggt ccgcagagta acctcggtaa
     1261 attgtgattt tactgtccgc tttatcagct tttaagccgc ttaatgcagt tataccttta
     1321 cagtacttaa ttgattctat aactttcttt gtctctccgc tttccggatt tttcaaaacc
     1381 tccttacagt tatacaaagc cagacccgtt ttacttattt catatctgac ggtggttatg
     1441 ttgagtcttt ctatttcctc tctgaacacc aaccttttat ccaataaagc cttggaatat
     1501 ctgtacggca tattcagcgg ttcctgtgca tccgaccttt taataactat atcgctgttg
     1561 aaagttgtga cattgaaatg tccgatattg caaaaaagtc tgttgcgaaa aagaaacaac
     1621 aatgctgcta caaccgctaa aacgattaca gtgatagtaa taatcaaacc tgtctttttc
     1681 tttttcacca tacactgaat gccctcaaat acctttacta acgctgttta tttaaatacg
     1741 acacaagtta cggaataatg cgtgtttaat aattaatccg cattgctttt aattgtatag
     1801 caatattcat aatccctgcc caatgcgttt ttctttcccg cacattttga tgtatcggtt
     1861 gccggtacaa aatactgctc cagatattcc agcaacggtg taatgtcggt atcttttttt
     1921 gtttctatgg ttacagagtt aatatagtca tttctgaaac gttcctttgt atacttatca
     1981 ttactacccg catatgttat atataccaca tcatatctgt catctgtctc gtaatagcct
     2041 attaccttaa aaccgactaa tactttctgg ttactctcca cagcatcatt tttctcgctc
     2101 aggtataaaa tatgggtttt taaatcggga agattacttg cggcatcatg cattgtctta
     2161 ggtattattg catatccttt tatatcgccg tcgcttttaa aggtactctc aatataatct
     2221 tcctttcttt gcacgacaag atccctgttt gtatagttcg tataaaaagt cacctcgctg
     2281 ttgctcttca ttctttctat ttcacttatg ctggtaatag gctgatgcaa aagatattga
     2341 acgtgttttt tccaagcttt cccttttcct acatctgcta aggcatcttt acgattctgt
     2401 gtaatcatat ttgttaccgc atcgcgaaaa ccggttgagc ttgaagcgaa acttatattc
     2461 agaccgtcag cattatcaag aataaaattt gtctgtataa aaccaccgcc tttgtctatt
     2521 gactcaagtt ttgcattttc ataaggatca tgtcctccca aaccggtact tatatttata
     2581 tcggttatgt attgtgagta tttattgtaa tctcttacca acgcatctgc atatacatag
     2641 gacatatcca aatcactttt tccgtctatt ctggaaacgg ttattatttt cttttcacaa
     2701 tctgtatcgg gtgataaatc caacgacttc atgttgacgg agttttcata catatatgag
     2761 taattctcgt taaacatgtt tatcctgcct tcatattgag aaagcgtgtt tgcatcggca
     2821 aaatatgtgg taagacagta ggtaagtttt gtgagatcgg cttgctcatc tgcgtcaatg
     2881 gccagacaat caacaaagct tatgtcaatc tcttcaccca tattaatcaa gttaccaaat
     2941 cccgcatatg acagatatag ggtatcgggc tcacctttat ttttgctcct gtcttttact
     3001 tcatactccc ctattatagt gaaatatgtt ataccgaaag cgcctttctc cagtactatt
     3061 cttttgtcgg aatcaggtat ctcttcgttt ataagctcac ctatggaaga cggaataatt
     3121 acataagctt tatctacact tgtaagatac tcctcggaat atcccgcata aaactccact
     3181 ttactttttc ctttgtcggc gactatcgtg cagagggaag ttataccttt acagtacttt
     3241 atcgagccca taacttccgc agctttttcc tcctccgaag aaacaaacaa atccctgtct
     3301 ccgcgcacac ttatatcatt atcctttatg ttgtatctgc gagaagatat atttacagcc
     3361 tgcactccgt caatatatgc tatattgtcc gaaagcaggg cttttgtata cttatacggt
     3421 atatttgccg gttgcttccc gtcaaccctt tttataacta tgtcgctgct gaatgttgca
     3481 tcaatcaaac cctcttcttt ttttgacaga ccgtcacaag atgccaacag caatgcagtt
     3541 attataactg ttaaggcgac agctataatc atatttgttt ttttcatgtt tttaccctcc
     3601 aatcggttgt ttgtaaatcc ttatgtaaaa cacaaattac ggcataattt ttactccgca
     3661 atctccttca ttgtaaaact gtattcatac gccaaaccca attcatttgt actgcctgca
     3721 tattcagcgg cgttttccga aggtgcaaaa tacaacttca aaaaattaat caacggactt
     3781 atctccacat ctccttttgt cttcatggtt atagactcta tatgttcgtt cttgaatgct
     3841 gcacttttat acttctcgtt gcatccgaca tatgttacaa acacggtatc atacggatcg
     3901 gttgtctcgt aataccctat tattttaaat tgggctctaa aatgaggaga tctgtctatc
     3961 tgactggggt caactccgcc tataccgaca tataccctgc tctcatatac ttcaacaatg
     4021 tgattgttaa aattgggaag atcggatgtt gcctcatgca gcggtttggg aactatagca
     4081 tagcctttta ttttgcccaa caagtccttc tgtctgggta caataaggtc actgttcata
     4141 tagttcagat agtatgtaac tttgcagtct tttttcatcc ttaatatttc atttatactt
     4201 gtaacggcct gatgataaca gggaaagtcc cccagcgttt ctttcagttt agcctcgtat
     4261 accgtgtcat attttaaaag caactgatat accggctgat tccagaaagc ttccgaaccg
     4321 ggaggataat ctgccggatt aattcctttt actcccgtac ttatatctaa atcggttatg
     4381 cattgtgaat atttgttata cccttttatt atcgcatcgg catacacatg cgacatttca
     4441 agatcttcct tcccgtccat tctggtaatg gttattatgt tcttttcata gattacatcc
     4501 tctttaagtt caataggctc tatactcatt gaatgaacaa acatatattg ataagggtcg
     4561 ttatatatat tattcctttc cgtatactgg gaaagcacct gtgcatccgc gtaatattcg
     4621 cttaagaaac gcataagttt attaagatcc ttgtcctcat tcacatcaat ttccagacag
     4681 tcaacatgat ttagaatatc ggttctgccg gcacggatta aggtacttaa gccggtatac
     4741 gtaacataca aggtgtcata ctcttcttca gtcttgtact cccctattat cgtaaaatgc
     4801 aacatgctgc cgtatctcat attccttata ttcaacacct tttcatcggc aggcaaatcc
     4861 ttattaaaat acttactcat ggaagaaggt ataatggcaa agttttgata atcatgaatc
     4921 agtaaatctt cggaatagcc ttcataaatg gttatcttgc aatcttcctt atcggcaact
     4981 atcgaactta aagtggatat acccttgaaa tactttacag catctattac ctgcttacct
     5041 ttacctctgt cggtatctgt caaaacaccc tccggtgtat gccaatcaat atagttatcg
     5101 ctcatgctat actttacgga tgctatattg atgccgacaa tttcctgtct gaataaaaac
     5161 atattatcca tcagtgcttt ggtatatctg taaggcatat ctaacgattc attcgcatcc
     5221 gttcttttta ttactatatc gctgttaaat gttgtaacaa caa
//
LOCUS       DAOPWC010000055         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15905_length_5218_cov_3.147782, whole genome shotgun sequence.
ACCESSION   DAOPWC010000055 DAOPWC010000000
VERSION     DAOPWC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15905_length_5218_cov_3.147782"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1097)
                     /locus_tag="PKJ65_02025"
     CDS             complement(201..1097)
                     /locus_tag="PKJ65_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ40649.1"
                     /translation="MLKRSLGEKIFNIFNLLGMFLFTLIIVIPVLFVFRNSFELSETA
                     TATLRLWPEDFSLVFYGMVIKDETVYRPLMNSVFVTVVGTPLTLLINALGAYTMSKRE
                     LPGKDFFAYYMVIIPMLIGGGTIPGFLLMRALGLIDSLWALIIPSLASGWNMTLLRNY
                     YWSIPNELTESARIDGASEMTIFAKIILPLSKPVLSALGLLSGLGFWNIFYAAMIYIR
                     TPSKWTFPVKIRETVMKNEGNDKLWEEMMVAAGFDPSELLINQSGLGGAMMVVSMLPV
                     LIVYPFLQKYFAGGIMVGSVKG"
     gene            complement(1114..2052)
                     /locus_tag="PKJ65_02030"
     CDS             complement(1114..2052)
                     /locus_tag="PKJ65_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ40650.1"
                     /translation="MDEKNLRIYKKNEKKIFTKVNIMIYLMIVPGLILYYIFRYVTMP
                     GIMIAFQEFTTGGFKYWCGFENFDKLFGLRAFWQAFRNTWVFIGLEYLLIRPAPIILA
                     LLLNEVRIMWFKKTVQTVSTLPSFLTWVVVSGIFIQLLSPSTGYVNVIIALLGGERIY
                     FLGKPQLFPWLLTGMRIWKGVGYSSIIYLASLGGVDPELYEAAVIDGASRWQQTMYIT
                     LPGIKNTIVVLLIFSFSGIMGGLFEPVFTLTDGNSLLDETAMVLDVYIYNTGIRQGKF
                     SMSTTTGLFNSIISTVLMLSANWASKYLTEDGRTII"
     gene            complement(2183..3178)
                     /locus_tag="PKJ65_02035"
     CDS             complement(2183..3178)
                     /locus_tag="PKJ65_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNZ40651.1"
                     /translation="MEILEKLTATFGVSGRENEINQVITDMVKNHVDRVMTDGIDNVI
                     AVKNGYGKNKKKIMVSAHKDEIGFMVMYIDSDGYIYLRPVGGIPVSVSIGNRIIFRNG
                     TVGVLCFKKDAESIKNNNVGDMFADIGAKNKEDAMNYVKPGDMGSYYGPLCMLKNDRV
                     VAKALDNRIGCYVAIKALMENERPYNDVYYVFTSQEELGLKGAIVASRVVQPEIAVAV
                     DVTSSFDTPITKEGNAVLGGGAAIKVMDRSVICDESLIGIMQDICKKNDIANQSDILA
                     AGGTDAGAIYTSNEGVRTGGISIPMRYVHGPVGMVDMNDVKACIELLKGFANQKI"
     assembly_gap    3247..3256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3257..4553)
                     /locus_tag="PKJ65_02040"
     CDS             complement(<3257..4553)
                     /locus_tag="PKJ65_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40652.1"
                     /translation="MFGKIKDYLRKLYIAVFYRNDTKLLTMPGKKDRDWFIVQGFVGT
                     VFALLTGATYLTGLFNKVNAPEIVIAYLPIVGTVAGVIAIFAGYIMQTIKNRKKFTLI
                     LNTILKTMIVLIAWIPLIFPNQNASYIMIAIALIAYALNNFMGITINSWFVDLIDVKI
                     RGQYMSVRNMIALVAHLLVPIIVSAFIDTSSDQYMAIVIVFTVSWLVMWAENYSFYKI
                     NEPPLQPPKKKIKLKDVLIVPLKDKTFMKSITSISVFYFIWYLSMSFATLYQLNYLGL
                     SYTFISISATLNVILQFIWYPVIGKLVDRHNTDFLLIVSYILYLIQAFLWGFTNISSR
                     YVMTILVNIFASMAIPLFSLVVFKAKYDVIPAEDRSLYDGFYTALIALIIAVAPAIGN
                     VLKDSISVNVRPFGIFEVPQFQLIFMLSGILLLGLILLNM"
     gene            complement(4555..>5218)
                     /gene="map"
                     /locus_tag="PKJ65_02045"
     CDS             complement(4555..>5218)
                     /gene="map"
                     /locus_tag="PKJ65_02045"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNZ40653.1"
                     /translation="KPGVTTFAIDKFVEQFIISHNAIPAQKGYHGYPYASCTSVNDEI
                     CHGMPSEYVLKEGDIVKVDMVADKDGWMADSAWCYAVGEVSPVAQKLMKVTKECLYKG
                     ISKAVEGNRLGDIGYHIQRHAEKNGFSVVRDFTGHGIGRAMHEEPAILHYGQIGRGLR
                     IVEGMVFTVEPMINEGDYDLYINRDNGWTAYTSDGSLSAQYEHTVAITANGPEIMTKQ
                     EF"
BASE COUNT         1506 a         1189 c          926 g         1587 t
ORIGIN      
        1 agggagtttt tagcttttct gcacatttca tgagggagtt ttagcaatct tcccacattt
       61 ttagaaagaa gtgctaaaat ctgtcttccg atcataggtt catccgctta aagctatcct
      121 taatattagt ttaatttttt tctttaaaaa aggatgagca ttcgcccatc cttttgttaa
      181 tctatatatt tcctaattga ttatccttta accgaaccta ccattatacc gcctgcaaaa
      241 tacttctgta agaaggggta tacaatcagt accggaagca tggatacaac catcatcgct
      301 ccgcctaagc ccgactggtt gataagaagc tccgacgggt caaatcccgc tgcaaccatc
      361 atttcttccc agagtttatc attaccttcg ttcttcataa ccgtttcacg aatcttaacc
      421 gggaatgtcc atttactcgg tgttctgatg tatatcattg ccgcatagaa tatattccag
      481 aatcccagac cggacagaag acccaatgcc gaaagaaccg gcttggataa cggaagtatt
      541 atctttgcaa aaatggtcat ttccgaagca ccgtctatac gtgccgattc cgtcaattcg
      601 ttaggtatcg accagtaata gtttctcaga agtgtcatat tccatcccga ggcaagtgag
      661 ggaataataa gtgcccagag ggaatctata agtcccaatg ctctcatgag aaggaatccg
      721 gggattgttc cgccgccgat aagcatgggt atgataacca tgtaataagc gaagaagtct
      781 tttccgggga gctctctttt actcattgta taagcgccta atgcgttaat aagcaatgtc
      841 aaaggtgttc ccactacagt tacgaatacc gagttcatta acggtctgta taccgtctcg
      901 tcttttataa ccattccgta gaataccaaa ctgaagtctt ccggccataa tctcagcgtt
      961 gccgtagcgg tctcactcaa ctcaaaagag tttctgaaaa cgaataatac cggaataact
     1021 atgattagtg taaataaaaa cattcccaac agattgaata tattgaaaat cttttctcct
     1081 aatgatcttt ttagcattta tctcaccttc ttcctatatt atcgttcttc cgtcttcggt
     1141 taagtattta cttgcccagt tggcacttaa cataagaaca gtcgaaatta tcgagttgaa
     1201 caaccctgta gttgtcgaca tactgaactt tccttgtctg ataccggtat tgtaaatata
     1261 tacatccaga accattgccg tttcgtcaag caacgagtta ccgtccgtca atgtgaaaac
     1321 cggttcgaat agtccgccca ttattcccga gaaagagaat attaaaagta cgactattgt
     1381 atttttgata ccgggcagag taatatacat tgtctgctgc cacctggaag caccgtcaat
     1441 aaccgccgct tcatataatt cgggatccac accgcccaag gatgcaaggt atatgatgga
     1501 ggaatatcct acgcccttcc atatcctcat accggtaagc aaccagggga ataactgagg
     1561 tttccctaga aagtatatcc tttcccctcc cagcaaagct ataattacgt taacatatcc
     1621 tgtactgggt gataacaact gtataaagat accgctgaca acaacccatg taaggaagct
     1681 cggtaatgtg gaaaccgttt gaacggtttt cttgaaccac atgattctca cttcattaag
     1741 cagtaatgcc agaataatcg gtgccggtct gataagcaaa tattccaaac cgataaatac
     1801 ccatgtgttt ctgaatgcct gccagaaagc tctcaagccg aacagtttgt caaagttctc
     1861 aaatccgcac cagtacttaa atccaccggt agtgaattcc tgaaacgcaa tcatgatacc
     1921 gggcatagtt acataacgga aaatgtagta cagtatcagt cccggaacta tcatcaggta
     1981 aatcataata tttaccttgg tgaagatttt cttttcgttt ttcttgtaaa ttctcaagtt
     2041 tttttcgtcc aattacaaca ccaacccttc ttttaaagat aaatccctcg gataatcgtg
     2101 cattataaca ttaatagttt atcacatatt taccgttttc aatacatgaa atttataact
     2161 tttgttgtat atatgtgatt tttcatattt tttggttagc aaagcctttt aacaattcta
     2221 tacaggcttt aacatcgttc atatccacca ttccgaccgg accgtgcaca tacctcatgg
     2281 gtatggatat accgcccgtc cttacacctt cgttggatgt atatatagct ccggcatcgg
     2341 ttccgcccgc agcaagaatg tccgactggt tggctatatc attctttttg catatatcct
     2401 gcattatacc gatcagcgat tcatcacaga taaccgacct gtccatgacc ttaattgccg
     2461 ctcctccgcc caataccgca ttgccttctt tggtaatagg agtatcgaat gaagatgtca
     2521 catccacggc aacagctatt tccggctgca caactctgct tgctactatt gctcccttta
     2581 agcccaattc ctcctggctg gtaaatacat aatatacatc attatacggt ctctcattct
     2641 ccatcaatgc ttttatggca acataacaac ctatcctgtt gtccaatgct ttggcgacca
     2701 ctctgtcatt tttaagcata cacaaaggac cgtaatagct acccatatct cccggtttga
     2761 catagttcat ggcatcttcc ttattcttgg ctcctatatc cgcaaacata tccccgacat
     2821 tgttgttttt aatactctcg gcatcttttt tgaaacataa tacgcccacc gttccgtttc
     2881 ggaatattat tctgtttcct atcgaaaccg atacgggaat accgcccacc ggtctgagat
     2941 atatatatcc gtcggaatct atatacatga ccataaatcc tatctcatcc ttatgggcgg
     3001 aaaccatgat tttcttcttg ttttttccgt acccgttctt aacggctatt acattgtcta
     3061 ttccgtcggt cataacccgg tccacatgat ttttcaccat atcggttata acctgattaa
     3121 tttcattttc tcgtccgcta actccgaatg tcgcagtaag tttttctaaa atctccacaa
     3181 ttttcccctt tcgttattcc tctttattta ttatcgtttt tcctgtccct ggcttctttg
     3241 aaaagcnnnn nnnnnntcat gtttaacagt ataagtccca acagcaatat gccggacagc
     3301 ataaatatca actgaaactg aggtacttca aatatgccga acggccggac atttacggat
     3361 atagaatcct tcagcacatt tccgatagcg ggagctaccg caattattaa cgcaatcaga
     3421 gctgtataga aaccgtcata taaagatctg tcttctgcgg gaataacatc atatttcgct
     3481 ttaaacacta ccagagaaaa cagcggtatg gccatggaag cgaaaatatt gaccagtatc
     3541 gtcattacgt atcttgagga aatattggta aatccccaca aaaaagcttg tattaaatat
     3601 aggatgtagc tcacgataag gagaaaatcc gtattatgtc tgtcaacaag ttttcctatt
     3661 acagggtacc agataaattg cagtatgaca ttcaatgtag cggaaatcga tatgaaagtg
     3721 tacgacaaac cgaggtaatt caactgataa agtgttgcaa aggacatgga cagataccat
     3781 ataaaataaa acacggaaat ggaggtaatg gatttcataa atgttttgtc tttcaaggga
     3841 actatcagca catccttaag ttttatcttt ttcttaggag gttgcaaggg cggttcattt
     3901 atcttgtaga aagaataatt ctccgcccac atgacaagcc aggatacggt aaacacaata
     3961 acaattgcca tgtactgatc ggatgaggta tcaataaatg ccgatacaat gataggaaca
     4021 agcagatgag ctactaaagc aatcatattc cttacactca tgtactgacc tcttatctta
     4081 acgtctataa gatccacaaa ccagctgttt atcgtaatac ccatgaagtt gttaagagca
     4141 taagcaatta aagctatggc tatcatgata tacgaggcat tttgattggg aaatatcaaa
     4201 ggaatccatg ctatgaggac tatcattgtt tttaagatgg tgttgagtat cagagtgaat
     4261 ttcttacgat tctttattgt ttgcatgata tatccggcaa aaatagctat gacacctgca
     4321 acggttccga caataggcag ataagctatc actatttcag gagcattaac tttgttgaaa
     4381 agcccggtca gataagtagc tccggtaagc agtgcgaaaa cagtacctac aaatccctgt
     4441 actataaacc agtctctgtc tttcttgccg ggcatggtaa gcaactttgt atcgtttctg
     4501 tagaataccg caatatataa ttttcgtaag taatctttta ttttgccaaa catattaaaa
     4561 ttcctgtttt gtcattattt cgggaccgtt ggcagtaatg gcaacggtgt gttcatactg
     4621 tgccgaaagg gagccgtccg aagtgtatgc ggtccaaccg ttgtcccgat ttatataaag
     4681 gtcataatcc ccttcattaa tcatgggctc aacggtaaaa accatacctt cgactattct
     4741 caatcccctg cctatctgtc cgtagtgcag tatggcgggc tcttcatgca tggctctgcc
     4801 tataccgtgt ccggtaaaat ccctgactac cgaaaaaccg ttcttttcgg catgtctctg
     4861 tatatggtaa cctatatcac ccagacggtt gccttctaca gccttgctta tgcctttgta
     4921 aagacactct ttagtaacct tcataagttt ttgtgcaacg ggacttacct ctcctacagc
     4981 ataacaccat gccgaatcgg ccatccagcc gtctttatcg gctaccatgt ccactttgac
     5041 aatatcacct tccttgagaa catattcgga aggcattccg tggcatatct catcatttac
     5101 cgatgtgcac gacgcatacg gatatccgtg atatcctttc tgagcaggaa tggcattgtg
     5161 tgatattatg aattgctcta caaatttgtc aatagcaaaa gttgttacac ccggcttt
//
LOCUS       DAOPWC010000056         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15930_length_5212_cov_4.170060, whole genome shotgun sequence.
ACCESSION   DAOPWC010000056 DAOPWC010000000
VERSION     DAOPWC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15930_length_5212_cov_4.170060"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKJ65_02050"
     CDS             <1..595
                     /locus_tag="PKJ65_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ40654.1"
                     /translation="KRLKTNEKSRLPYKVMQAPITDQPLLELAFAVIFPAFGQAILFN
                     IGASSGGTDIVAMLLKKFTHWDIGRSLFISDCILTLAGFAVFSVETVLLSILGLLIKS
                     TLVDSVIENLNLNKYFTIITEHPEEICDYILNHIKRSATVFDGKGAFTNENKKIIITV
                     MSRSQAVQLRQYIKQVDPGAFILITNTSEIIGKGFRA"
     gene            624..1955
                     /locus_tag="PKJ65_02055"
     CDS             624..1955
                     /locus_tag="PKJ65_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40655.1"
                     /translation="MKSTGDFGFLPGNNARANTTALQKAVDKTGTIRIEQPGIYDIDD
                     TVYIPSDTALVFGKGVYIRRTTDRGYVFINKGAYTREYDYNISITGLNLLCNGHNSKE
                     GNLIVGLRGQVCFFYVKDLVIRDLLCLDLTEHSFCIHICTFENLLIENVHIEGLKDGI
                     HIDKGSKFTIRHGVFKTFDDPIALNAHDYTSCAPELGWIEDGIIEDCYDLDQEKTTGY
                     FCRLLAGSWLDWFKGMKVQQSDTVVYNNKIYRVNMQPDGKIYTSNTAPNHSEGTKEYD
                     GIRWTMLQDREIVYNCGCRNIHFRNIFLKKKRHMAFALTFDKNEYSRGYYPNSIPPVQ
                     EDITFERIYVQNEIIDLLGIRTPVNTIKLTDSVIGNNRIYLRYLEEEGITYIPAHVLI
                     SNVTFKGDGKQSLVYAEKGRKATLKIKGSIKENASYEPLIEGDIEILKKDI"
     gene            complement(2106..3380)
                     /locus_tag="PKJ65_02060"
     CDS             complement(2106..3380)
                     /locus_tag="PKJ65_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ40656.1"
                     /translation="MDKELYKPRLVDKQVKLELEAFGAVCIEGAKWCGKTWTSGYHSK
                     SAIYLGDPSRNFQNRELAKLEPTTVLEGEVPRLIDEWQEVPSIWDAVRHEVDRRGKKG
                     QFILTGSSTPKQKGILHSGAGRISTIRMRPMSLFESNDSSGQVSLTSLFDGTFKAVAT
                     GNIDLETLIKLTIRGGWPESLGLDYEKFRLLPKEYIKSIINHDIYRLEGINRDSAKMH
                     LLLKSLARNESTTASIATLKKDISDFDNDTLDNDTISTYLSTFERLFLIENQKAFSSN
                     IRSSTRIKQSEKRHFVDPSLAIAILEASYEDLLNDLHTFGFMFEALCVRDLRIYAESL
                     EAQLYHYQDYKEREIDAVVKLRDGRFGAFEIKLGADAIEKAAQSLIKIRDDLKAEGTR
                     IPDILVVICGLSNFAYQREDGVYVVPITALRP"
     gene            complement(3669..3992)
                     /locus_tag="PKJ65_02065"
     CDS             complement(3669..3992)
                     /locus_tag="PKJ65_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40657.1"
                     /translation="MTKVMIDPGICNLVTSVEASSDDGMEVTLKVKSGCKSINDMFKD
                     LGDTFDAYELCLGKPGSGPLYDYAKEHLPGHCGCPAIAGIIKAVEVECNLALPKDAKI
                     TFVKE"
     gene            4157..>5212
                     /locus_tag="PKJ65_02070"
     CDS             4157..>5212
                     /locus_tag="PKJ65_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNZ40658.1"
                     /translation="MGRYTQIISQRLIPLPKELSCNDIKKVAFNDIKIDVIYPEDALL
                     RSAKRKLVSFFGNSLYGSFKIRIVDPMPETLNVLNHFPNSDQAYRIWSDGSINISALT
                     SKGIMNGVRTLLQAVSKEDGMFNIPLLFIYDYPDIPLRGQRSETAVRDIAYMASLKLN
                     SADYMSINSIDEEKRLKENCINEGIVLNSNISYSGCITDENLCVLSENTDTVIFDWMT
                     YGGKCIFPRTAVDFIKENCEKFVENKTRKVVCYTPTSDIYQKFNITAMAEWLWNAKSR
                     DAESFMRAYATVNQMDSDKFVKWCTYNMTPARTLAESGFIERLMSNRTVTVKGKEYLD
                     YCGFDDFIENAIIDIDKY"
BASE COUNT         1627 a          990 c         1018 g         1577 t
ORIGIN      
        1 caaaaggctt aagaccaatg aaaaaagcag gcttccgtat aaagtcatgc aagctcccat
       61 aaccgatcag cctttattgg agttggcttt cgccgtcata tttcccgcat tcggtcaggc
      121 aattttgttt aatatagggg cgtcttccgg cggtaccgat attgtggcaa tgttgctgaa
      181 gaaatttaca cactgggata tagggagatc gctgtttatt tccgattgta ttcttacatt
      241 agcgggattt gccgttttca gtgtggagac ggtactgttg tccatattgg ggctgctgat
      301 aaaatccact cttgttgaca gtgtaataga gaatcttaat ctgaataaat actttacaat
      361 cataaccgag catccggaag agatatgcga ctatatactt aatcacataa aaaggagtgc
      421 caccgttttt gacgggaaag gcgcatttac caatgagaac aagaaaataa tcatcaccgt
      481 aatgagcaga tcgcaggcgg tgcagttaag acaatatatt aaacaggtag atcccggagc
      541 ctttatactt atcaccaata cctcggagat tatagggaag ggttttcgtg catgattgcc
      601 atacataaag tgcggaggat ataatgaaaa gtaccggaga tttcggtttt ttaccgggta
      661 acaatgcaag agccaacaca acagctttac aaaaagcggt cgacaaaaca ggaactataa
      721 gaatagaaca accgggcata tacgatatcg atgataccgt ctatatacct tccgataccg
      781 cactggtatt cggtaagggt gtgtatataa gaaggactac cgataggggc tatgtattta
      841 tcaataaagg agcctataca agagaatatg attataatat ttctataacc ggacttaatc
      901 ttttatgtaa cggacacaac tccaaagagg gtaatctcat agtcggcctt agggggcagg
      961 tatgtttctt ttatgttaag gatttggtta tacgtgacct tctctgttta gatcttacgg
     1021 aacacagttt ctgcattcat atatgtacat ttgagaatct tttaatagag aatgtgcaca
     1081 tagaagggct taaagacggt atacatatag ataagggcag caagtttaca ataaggcacg
     1141 gggtatttaa aacattcgac gatcccatag cattaaatgc ccatgattac acaagctgtg
     1201 ctccggaact tggctggatt gaagacggca taattgaaga ctgttatgat ttggatcagg
     1261 aaaagactac aggctatttc tgcagactgc ttgcagggtc ctggcttgac tggttcaaag
     1321 gaatgaaagt tcagcaatcg gataccgtcg tatacaacaa taagatatac agggtaaaca
     1381 tgcagccgga cggcaaaata tatacatcaa acaccgctcc aaatcacagc gaaggaacaa
     1441 aggaatatga cggtataagg tggacaatgt tacaggaccg agaaattgta tataactgcg
     1501 gatgcaggaa tatacatttt aggaatatct tcttaaaaaa gaaaagacat atggcatttg
     1561 ctctgacctt tgataagaat gaatattcca gaggatatta tccaaactcc ataccgcccg
     1621 tgcaggaaga tataaccttc gagcgtatat atgtacagaa tgaaattatc gacttattag
     1681 gtattagaac gccggtaaat acaataaaac ttaccgattc ggtaataggt aataacagga
     1741 tttatctcag atatcttgaa gaagagggaa taacatatat ccccgcacat gtacttatat
     1801 ccaacgtaac gtttaaaggt gacggcaaac agagccttgt atatgcggaa aaaggaagaa
     1861 aagcgacatt aaagattaaa ggctccataa aagagaatgc ttcctatgaa cctttaatag
     1921 aaggggatat cgaaatactg aaaaaggata tatagtacgg acatattccg ccgacttgca
     1981 catcaaaaca gcgccgactt gcacatttgt gttgctgtca ggaactgatg atgtgtatat
     2041 gtaatgccta ttcggtgttt gaagataaat gctcggacgt aaccatttta caattttcaa
     2101 cggctttatg gtctcagcgc ggtaatcggt actacataaa ccccatcttc cctttgataa
     2161 gcaaaattgc ttaatccgca tatgaccacc aaaatatccg gaattcttgt tccttcagct
     2221 tttaaatcat ctctgatttt gattagtgat tgtgcagctt tttcaatagc atctgcaccg
     2281 agctttattt caaaagcccc gaaacgacca tctctaagtt tgacaactgc atcaatttct
     2341 ctttccttgt agtcttgata atggtacagt tgagcttcca aggattccgc gtatattctt
     2401 aaatctctta cacatagcgc ttcaaacata aaaccgaatg tgtgcagatc gtttaataaa
     2461 tcctcataac ttgcttcaag tatcgctata gctaaagaag gatcaacaaa atgtctcttt
     2521 tccgattgct taatgcgtgt tgatgacctt atattacttg aaaaggcctt ttggttttct
     2581 attaagaaca gtctttcaaa tgtacttaaa tatgttgaga ttgtatcatt gtcaagagta
     2641 tcgttatcaa aatcggaaat atccttttta agagtagcaa tagaagctgt tgtcgattca
     2701 tttcttgcaa gagattttaa tagcaaatgc attttggccg agtctctgtt aattccttcc
     2761 agtcgataaa tatcatgatt tatgattgac ttaatatact ctttcggaag tagcctgaat
     2821 ttttcataat ccaatcctaa actttccggc caaccgcctc ggattgttaa ttttataagg
     2881 gtttccaaat ctatattgcc ggtggctact gccttaaaag taccatcaaa caaagaggtc
     2941 aatgatactt gtcccgatga atcattcgat tcaaataatg acatcggtct cattcttatc
     3001 gtagatattc tgccggcacc gctatgcaaa attccttttt gcttcggtgt tgacgagcct
     3061 gttaaaataa actgtccttt tttacctctt ctgtctactt catgtctgac tgcatcccaa
     3121 atagaaggca cttcctgcca ttcatctatt aatctgggta cttctccttc taaaacagtt
     3181 gttggttcga gctttgccaa ttccctgttt tgaaaatttc tcgacggatc tcctaagtat
     3241 atggcgcttt tggaatggta tccggaagtc catgttttgc cacaccattt agctccttca
     3301 atacaaacgg cgccaaaggc ttcaagctcc agttttacct gtttatcaac taatctcggt
     3361 ttatatagct ctttatccat agttacctcc gacacgagta taacatgaat cggtcatatt
     3421 ttcaatttct attcggttaa attttcaatt tctattcggt taaatttgca atttctattc
     3481 ggtcatattt tcaatttaca ttcggtcatt ttttggaata ttaaacttga ttaatgtttt
     3541 tcgacggttt catttaatgt ttttcctgca tatcgacgat ttcatttaac gtttttcgac
     3601 aatatcaatc agtgtttttc ggagtattga ttagaaacaa tgtatatttg attattatga
     3661 aagtacggct actctttcac aaatgttatc ttggcgtctt tgggtaaagc cagattacat
     3721 tcaacttcca ccgcttttat aatccctgct atagcgggac atccgcagtg gccgggcagg
     3781 tgctcttttg catagtcata taaaggtccg cttcccggtt taccgagaca cagttcatat
     3841 gcgtcaaatg tgtcgcctaa gtccttaaac atatcattaa tgcttttgca tcccgattta
     3901 actttcagtg taacttccat tccgtcatcc gaggatgcct ctaccgatgt gacaaggttg
     3961 catatacccg gatctatcat tacttttgtc atattacaga atatcctttc aaatgtttta
     4021 catgaatgct attttacacc aatatacagt cgtatggaac atgtgacagt gatattatca
     4081 cttccctaat atgtgtaaga ctgtaaatca ttacatgaac atgatagaat tcatgtataa
     4141 aaccgggggt gttgtcatgg gcagatatac acaaataata tctcaaagac ttattccttt
     4201 accgaaggag ttgtcatgca atgacattaa aaaagtggca ttcaatgaca taaaaataga
     4261 tgtaatatac cccgaagatg ctttactgcg ttcggctaaa agaaagctgg tatcattttt
     4321 cggcaattcc ctttacggct ctttcaagat aaggatagtg gatcctatgc cggaaacctt
     4381 gaatgtgctt aaccattttc cgaattccga tcaggcatat cgcatatggt ccgacggatc
     4441 aataaacata tctgcactga cttccaaagg aataatgaac ggagtacgca cattattaca
     4501 agccgtatca aaggaagacg gcatgttcaa tataccgctt ctgttcatat atgattatcc
     4561 ggacataccg ttaagaggac aacggagcga aacagccgtg cgggatatcg catatatggc
     4621 ttctttaaaa ctcaacagtg cggattatat gtcaattaat tcaattgatg aggaaaaaag
     4681 attgaaggaa aactgcataa atgaaggtat agtgctcaac tccaatatat cttattccgg
     4741 atgtattaca gatgaaaatc tttgtgtttt atcggaaaac acggataccg ttatttttga
     4801 ttggatgact tacggcggaa aatgtatatt cccccggacc gctgtagatt tcataaaaga
     4861 aaactgtgaa aaatttgttg agaataaaac acgcaaggtt gtatgttaca caccgacatc
     4921 cgacatatat caaaagttca atataacggc tatggcggaa tggttatgga acgcaaaaag
     4981 cagggatgcg gaaagcttca tgagggcata tgcgaccgtc aatcagatgg attccgacaa
     5041 attcgtaaaa tggtgtacat ataacatgac tccggcacgg acgcttgcgg aaagcggatt
     5101 tattgaaaga ctgatgtcca ataggactgt gacggtaaaa gggaaagaat acttggatta
     5161 ctgcggattt gatgacttca tagaaaatgc gattatagat attgataaat at
//
LOCUS       DAOPWC010000057         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_15932_length_5212_cov_3.342641, whole genome shotgun sequence.
ACCESSION   DAOPWC010000057 DAOPWC010000000
VERSION     DAOPWC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15932_length_5212_cov_3.342641"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1511
                     /locus_tag="PKJ65_02075"
     CDS             243..1511
                     /locus_tag="PKJ65_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNZ40659.1"
                     /translation="MIYAIILFVITYILMLTLGKYRTYVALISAAVFIISGMLPLNKI
                     FGSIDFNVLLMIAGTMGLVQLFIDSKMPALLADLVMEKIPNVQMATVALALFAGIISA
                     FVDNVATVLMVAPIALEICKKLKTNPVPFIIAIAVSSNLQGAATLVGDTTAIMLGSYA
                     NMNFLDFFFYQGKPGIFFAVELGALLSALILAWLFRKEKQPIPKDTARTVVTDYVPTV
                     LLVGMIVLLIAASFIPDKPDITNGLICVGLLIIGIIYTAIKTSDKKSIITILKAVDLE
                     TIGLLFGLFLIIGGISHMGVIDAVAGLLAKLGGGNVFVLYTVIVWASVLFSAFIDNIP
                     YVATMLPVISGLAANLGIQPTLLYFGLLSGATLGGNCTPIGASANIAGIGILRKAGYE
                     VKTKDFVRIGIPFTLAAVIPAYIYFWLLYG"
     gene            1637..3427
                     /locus_tag="PKJ65_02080"
     CDS             1637..3427
                     /locus_tag="PKJ65_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ40660.1"
                     /translation="MKLTTETTRCDLCVVGGGISGVCAAIAAARRGLKVVLMQERPVL
                     GGNASSEIRMWICGCKGLNNRETGILEEIQLDSLYRNPYKLYPVWDSLIYNKVISEPN
                     ITLMLNCSCCKADMDKDKIQAVYGWQMTSQTWQKVEADYFADCSGDSILAPLTGAEYR
                     MGRESKEEFGEDIPLTEPDSKTMGNSCLIQARKLDHEVTFKAPPFATKLTREDVEKRH
                     PNIESSYENFWYLELGGDRNTIKDAESIRDELVALAYGMWDYIKNSGDYDAGCWQLDF
                     IGFLPGKRESRRLMGDHIMTQKEISADTFFPDTIAYGGWPLDDHDPGGFWHPGRASSI
                     IPTPKPYKIPLSVLYSRNIDNLWFAGRNISMTHAAMSSSRIMATCALLGQAVGESCAV
                     AKKYNLSPRLARKLHISEIQQNLMENDCFLPGFIREISPLCKKATLSSDPHIENLDNL
                     RSGTDRDHQIYDGPQGVYIRKGGSIEYTLEKATKISKVRMVFDSDLDRNTLPGDLCER
                     THTMRASINIDAPVMTMPKTLVKSYKLTAQLEDGSETVLIEDTENIKRLIKVNAGKKI
                     KRLTLTLLSDWSENGAYFDDKIHLFSFDFS"
     gene            3616..>5212
                     /locus_tag="PKJ65_02085"
     CDS             3616..>5212
                     /locus_tag="PKJ65_02085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ40661.1"
                     /translation="MKKVNEITFFILIAAIFISTFVLTDVLDMPNLYLIDREGVSFID
                     GWTWKGDGYKERYYLPHRFDVEKSEPLVIKNTVPDRFPAGSVIALKANVQSVVIKIDG
                     ETVYDIGNDRSKFMGRDLGNYWAVVKTQSEHKGKEIEISLFSHRAASHGFASEVFIGS
                     ESAFYGYLFAQHGLWNIFPPIIFILGIMIILTFFFLGMYKEKNLSLLYLGIYALLMSN
                     WFLGDSEMLQLFTANTYYSTRIAYLMTLVSPIPVSLYIREAVPMKKRFFDDFLIGLII
                     INTVVSLGLEHSGILGLSDTVLVAVGLISVMCVYYMAIFIIESVAYKNEKALRELKSL
                     SVIFIFAVAEIISYFVNGQIVSSLYLRIGVLIYIVLVLIYQVKDYRERRRIRLEKEYY
                     EKMAYTDAMTGAGNRARFMEDMDMITKPERVIIVLADIDRMKYINDYFGHFSGDKAII
                     DTYNTLNTNFGHIGRIYRIGGDEFVVIVEKTNINEIYAIIKNLEKEVDLIDKESEYDF
                     SISLGVAEYDASLDEDIHATFLRV"
BASE COUNT         1526 a         1012 c         1153 g         1521 t
ORIGIN      
        1 cctccatgta tgaagataag aggagacata ttgccgaaag agtaattccc gacaggagaa
       61 aaagtcagta aaagactgca atagttttct gcaagaaact cacagggtga aaacaaatct
      121 atagaaaatc gctttctaca ccgttttttt acttagttta tattgctttg tagtataatt
      181 aagcaggtta tatagtgatg gaccgttaaa gattttaatg taaagggaga aaaggttttc
      241 ctatgattta cgcgattatt ctttttgtta ttacttatat actgatgctg actttaggaa
      301 agtatcgtac atatgtcgca ttgatttcgg cagccgtttt tattatctcc ggaatgctgc
      361 ctcttaacaa gattttcggt tccattgact tcaatgtgct cctgatgatt gccggtacca
      421 tgggcctggt tcaactgttc attgacagta aaatgccggc acttctggct gatctggtta
      481 tggaaaagat tcccaatgtt cagatggcta ctgttgcact ggcactcttc gcaggaatca
      541 tatccgcatt cgtggataat gtcgcaaccg ttttaatggt agctcccata gcactggaga
      601 tctgtaaaaa actgaagacc aatccggttc ccttcattat tgctattgcc gtgtcatcca
      661 atctgcaggg tgctgccact ttggtcggcg acacgaccgc cataatgctg ggttcatacg
      721 caaacatgaa tttcttggac tttttcttct accaaggtaa accgggtatt ttctttgcgg
      781 ttgagttggg tgctctttta tccgcattga ttctggcatg gctgttccgc aaagaaaagc
      841 aaccgatacc caaggatacc gcccgtaccg ttgttaccga ttatgttccc accgtgttgc
      901 tggtcggtat gattgtcctt ctgattgccg cttctttcat tcccgataaa cctgacatta
      961 caaacggtct tatctgtgtc ggattgctta ttataggcat tatttatacc gccataaaaa
     1021 cttccgataa gaaatcaatc ataactatac tgaaagcggt ggatctggaa accataggcc
     1081 tgttattcgg tctcttcctt ataatcggcg gtatttccca tatgggcgta attgatgccg
     1141 ttgcggggct gctggccaaa ctcggcggcg gaaacgtatt tgttctttat acggtcattg
     1201 tatgggcttc ggttttgttt tccgcattta ttgataacat tccctatgtt gcaaccatgc
     1261 tgccggttat cagcggtctt gccgccaatc tgggaatcca accgactctg ctctatttcg
     1321 gactgctctc cggtgccacg ctcggaggaa actgcacacc tataggtgcc tctgcgaata
     1381 ttgcgggtat cggtatcttg cggaaggccg gttatgaagt aaaaactaag gattttgtcc
     1441 gcataggtat tccgtttact ttagcagcgg taattcccgc atatatatat ttctggttgc
     1501 tttacggtta aaggacatcg gtgttacaaa agatgcatta aattaaccgc aaccttactt
     1561 aaaatggtat tatattatca gtataataaa atgagtcgat taaatattta attgtaaaga
     1621 gcaaggagaa aaacccatga aattaacaac cgaaacaacc cgctgcgatc tctgtgtagt
     1681 cggaggcggt attagtggcg tatgtgctgc aatcgcagca gctagacgag gattgaaagt
     1741 ggtcttaatg caggagcgtc cggtgctcgg aggcaatgct tcttctgaga taagaatgtg
     1801 gatatgcggc tgcaaaggtt taaataaccg tgaaaccggt atacttgaag aaattcagct
     1861 ggattcactg tatcgcaacc cgtataagct ctaccccgtg tgggacagct taatttacaa
     1921 caaagttatt tccgagccga atattacact tatgcttaac tgctcctgct gcaaagcgga
     1981 tatggacaaa gataaaatcc aggctgtata cggctggcag atgacctcac agacatggca
     2041 gaaagtagaa gcagattact tcgccgactg ttccggtgac agtattttag caccgcttac
     2101 gggagccgaa tatcggatgg gtcgcgagtc taaagaggaa ttcggcgaag acataccctt
     2161 aaccgaaccg gacagcaaaa ccatgggtaa ttcctgcctg attcaagcca gaaaactgga
     2221 tcatgaagtt accttcaaag cccctccgtt tgcaacaaaa ctgacacggg aagatgtgga
     2281 aaagcgtcac ccgaatattg aaagcagtta tgaaaatttc tggtacctgg agttgggagg
     2341 agacagaaac actatcaaag atgccgaatc catccgcgat gaacttgtgg ctcttgccta
     2401 cggaatgtgg gattatatta aaaacagcgg ggattatgac gcaggttgct ggcagctgga
     2461 tttcatagga tttctaccgg gaaagagaga atccagaagg cttatgggtg accatataat
     2521 gacacagaag gaaatatctg ccgatacctt tttccccgat acgatagctt acggcggctg
     2581 gccgctggac gatcatgatc ccggaggctt ctggcacccg ggacgtgcca gttccatcat
     2641 acccacaccc aaaccgtata agattccgct ttcggtgctt tattcaagga atatagacaa
     2701 tctgtggttt gcgggaagga atataagcat gacacatgca gcaatgtctt catcacgtat
     2761 aatggcaacc tgtgctctgt tgggacaggc tgtaggagag agttgcgcgg tggctaaaaa
     2821 atacaatctg tcgccgagac ttgcccgtaa acttcacatc tcggagattc agcagaactt
     2881 aatggaaaac gactgcttcc tgccgggatt tataagagaa atctctccgt tatgtaaaaa
     2941 agcgacatta tcgtccgatc ctcatattga aaacttagat aatcttagaa gcggaacaga
     3001 ccgtgaccac cagatttacg acggtcccca aggagtgtat ataagaaagg gcggatcaat
     3061 tgaatataca ttggaaaaag caacaaaaat ttcaaaagtc cgtatggttt tcgactccga
     3121 cctggacaga aatacgttgc ccggtgatct ctgcgaacgg acacacacca tgcgggcaag
     3181 cataaacata gatgcacccg tcatgacaat gcccaagaca ctggtaaaat cctataaact
     3241 gaccgcacag cttgaagacg gcagcgaaac cgtgctaatt gaagacactg aaaacatcaa
     3301 acggttaata aaagtaaatg caggtaagaa gattaaacgt cttacgctga cgcttttatc
     3361 cgactggtcg gagaacggtg cctattttga cgataagatt catctcttct cttttgattt
     3421 cagttaagtt taatttgcct gaacgggata ctatttgtag tttttgacat gattacatga
     3481 aaacaaataa agctgataat attaatcatt tatctcaata aaactatcgc agtaagcgtt
     3541 gtttgacaaa ccctaccatc acagtcataa tataactgtc attatgtggc agcgacgaga
     3601 aggagtgagc agtacttgaa aaaggttaat gagataactt tttttatact aatagccgct
     3661 atatttattt ccacatttgt tcttacagat gtcttggata tgccgaatct ttatctcatt
     3721 gacagagagg gagtttcttt catagacggt tggacatgga aaggagacgg ttacaaagaa
     3781 agatactatt tacctcatcg ttttgatgta gaaaaatccg agcctttagt gataaagaac
     3841 actgttcccg accgcttccc cgccggatcg gttattgctt tgaaagccaa tgttcagtcg
     3901 gttgttataa aaattgacgg agaaaccgtc tatgatatcg gcaacgacag aagtaagttt
     3961 atgggaagag atttaggtaa ctactgggca gttgtaaaga ctcaatcgga acataaagga
     4021 aaggaaattg aaatttctct cttctcccac agagccgcct cacacggttt cgcttcggag
     4081 gtttttatag gttcagagag tgccttttac ggttaccttt ttgctcaaca tggtctttgg
     4141 aatattttcc ctcctattat tttcatttta ggaataatga ttattttaac tttctttttc
     4201 ctgggtatgt ataaggagaa aaatctaagt ctcctttatt tgggaattta tgctttacta
     4261 atgagtaatt ggtttttagg tgattcggaa atgttgcagt tatttaccgc aaatacatat
     4321 tactccacca gaattgcgta tctcatgaca ttggtttccc ctatacccgt aagtttgtat
     4381 ataagggaag ccgttcctat gaaaaagaga ttttttgatg actttttaat aggcttaata
     4441 attataaata cagtcgtcag cctggggctg gagcattcag gcatattagg tctttcggat
     4501 acggttctgg ttgcggtggg acttatatcg gtaatgtgtg tatactatat ggcgattttt
     4561 atcatagaat cagttgctta caaaaacgag aaagctttgc gggaactgaa atcgctttcc
     4621 gttattttca tatttgcagt tgcggagata atatcatatt ttgtaaacgg acaaattgtt
     4681 tcctctctct atctgcgtat cggcgtatta atatatatag tcttagtgtt gatttatcag
     4741 gttaaggatt acagagaaag aagaaggata cggttagaaa aagaatacta tgaaaaaatg
     4801 gcatataccg atgccatgac cggagcgggt aatcgtgcaa gatttatgga agatatggac
     4861 atgattacta aaccggaaag agtcattatt gtgctggccg atattgacag aatgaaatat
     4921 ataaacgatt acttcggtca tttcagcggt gacaaagcta ttatcgatac ttacaatact
     4981 ttgaacacaa acttcggtca tatcggcagg atttatcgca taggcggaga tgaatttgtt
     5041 gtaatagttg aaaagaccaa cataaatgaa atatatgcga taattaagaa cttagaaaag
     5101 gaagtggatt taatagataa ggaaagcgaa tatgactttt caatatcttt aggtgttgct
     5161 gaatatgatg cctctttgga tgaggatatt catgctacct ttttaagggt tg
//
LOCUS       DAOPWC010000058         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_16028_length_5192_cov_2.518201, whole genome shotgun sequence.
ACCESSION   DAOPWC010000058 DAOPWC010000000
VERSION     DAOPWC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16028_length_5192_cov_2.518201"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKJ65_02090"
     CDS             <1..279
                     /locus_tag="PKJ65_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNZ40662.1"
                     /translation="TVDITRYSILINDVPTHATPKEVEVLFLLLSNPRIVFTREQIMN
                     LIWGYEYTGDTRTIDTHIKRIRAKLPAEIQKCIQTIYGVGYKFECNEE"
     gene            269..2119
                     /locus_tag="PKJ65_02095"
     CDS             269..2119
                     /locus_tag="PKJ65_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40663.1"
                     /translation="MKNKFTDIPFFVRIVLLILSILIISLIITTVIYTVASEEIFISN
                     QLERLKEETDMLASLIPGDWVTRDQLEYFFDKALQAESTILRSFIFVSDRLNVEVYSY
                     APAIKRETQTVAKADMDKFYRNNLSKHMRGVLAGQYISLKNTDNEFHSDMFIIMRPIF
                     SQYGDVQAVLTVCKFIDDYSGELRTINNALVLTMIGVSLLMLFPIYRMSKSITKPLLE
                     ITDVAVAFGKGDFSVKADQSYPAELGQLSLTMNHLSSQLSKTITSLTRETNILQGVLN
                     SMNQGILLVDETLSPMIINPALPKFFKSTMDINEKHSIIPLKAVWEDFEQCLSEQQYI
                     ENIYEYDGKMILCVINPLKVNDALIGAMGIFSDITKEIRLEQTRRDYVANVSHELKTP
                     LTGVMGLLAPLKDGIVTDPDKITKYYAIMSNELQRLNRLINDLLMLSRLQSASEAFAM
                     DKVNLSAIIVDKIENYHFAYEDNNLAIDFTGPSQPVLVYGNADRIEQILTILIDNAIK
                     YSQNSSDKTIINEKIKIKIAIVKKDDKYIVSVQDYGTGISKEDIEYVFERFYKSDKSR
                     SGAGSGLGLAIARETLKRMNEDIWVESDGKSGSVFYFTLTEYKPNIDRVK"
     gene            complement(2127..3338)
                     /locus_tag="PKJ65_02100"
     CDS             complement(2127..3338)
                     /locus_tag="PKJ65_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNZ40664.1"
                     /translation="MRLFNKKKIYLFDGSKGFMLQKYGLQKGELAESYNITHPDTVMR
                     IYKEYCNAGSDIIQTNTLCSNRLVLKRHGLEELHDKINSQGIELAKQAIDGRNILIAA
                     SIGPIGELLQPFGPLTFEYAYSLYKDQIYACRAADVINFETFTDLKEMRIAYLANRET
                     VNLPVICNMTFEKNNMTLMGHSPKVCAGILKKMGADVVGVNCSNGPDKMGDILKDIAL
                     FEDFVCVKPNAGVPSFVDGIAHYDQGKIEFCGYSDNLLDFNVGIIGGCCGTTPEYIKA
                     MNYIKDKDRSITVPSDKKRYIFCQYGYVDVDDSYETVDFAVSDGDVYDNIDAAYDINE
                     EPCDAFNIFYSENNAQFLFELINQIQDILKKPFIFHIDDKVCLDSALRAYCGIAGIRG
                     FELCKYGAVKI"
     gene            complement(3356..3892)
                     /locus_tag="PKJ65_02105"
     CDS             complement(3356..3892)
                     /locus_tag="PKJ65_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNZ40665.1"
                     /translation="MNRNKILLHTCCAPCLVYVHEKLNEMNVDYDSYFYNPNIHPIKE
                     HERRLLTLRSYMSRFNIPLIEDDEFLQHLWEEGFQCKDCYSIRLDKTAKYAKDNGYKG
                     FSTTLLVSIYQNHQLIIDIAESVADKYGIEFFYHDFREGYRIGQNKAKEYGLYRQKYC
                     GCINSYNSSEYKDKIKWD"
     gene            complement(3879..4880)
                     /gene="ruvB"
                     /locus_tag="PKJ65_02110"
     CDS             complement(3879..4880)
                     /gene="ruvB"
                     /locus_tag="PKJ65_02110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ40666.1"
                     /translation="MEEERFVTPSFIKNDQSEASLRPKVFDEYFGQEKIKENLKVFIE
                     AAKLRREPIDHVLLAGPPGLGKTTLAGIIASEMGANIRITSGPAIEKAGDLAAILTNI
                     EENDILFIDEIHRLNRTVEEILYPAMEDFALDIIIGKGPSAKTIRLDLNKFTLIGATT
                     KTGMLSSPLRDRFGVSCRLEMYTVEELSKIVKRSAGILGFNINEYGANEIASRSRGTP
                     RIANRLLRRIRDFAQVEGEDVITSEIADYGLQKLEIDKIGLDNIDRAILETIITKFNG
                     GPVGLETLAASINEEKDTIEDVYEPYLLQLGFIKKTPKGRCVTSHAYEHLRIPYEPK"
     gene            complement(4893..>5192)
                     /locus_tag="PKJ65_02115"
     CDS             complement(4893..>5192)
                     /locus_tag="PKJ65_02115"
                     /inference="COORDINATES: protein motif:HMM:NF019140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ40667.1"
                     /translation="EVATAVVSEDAASLSKAPGVGKKTAMRIILELKDKLKGLYAAEA
                     DAVKQIAVSDKYSEAVNALLVLGYNTKYAREALNKVYDESDSLQDIIKKALAEQI"
BASE COUNT         1511 a         1118 c          929 g         1634 t
ORIGIN      
        1 accgtggata taacaaggta ttctatcctt ataaacgatg tacccacaca tgccactccc
       61 aaagaagtgg aagttctttt tcttcttctg tccaatccca gaatagtatt taccagagaa
      121 cagattatga acttaatctg gggctatgag tatacaggcg acaccaggac cattgacact
      181 catatcaagc gcatacgtgc aaaacttcct gcggaaattc aaaaatgtat acaaacaatc
      241 tacggagtgg gatacaagtt cgagtgtaat gaagaataag tttaccgaca tacctttttt
      301 tgtaagaata gtattactaa tactatcaat acttattatt tcacttatta tcaccactgt
      361 catttacacc gttgcttcgg aagagatatt cataagtaat caactagaga gattaaaaga
      421 agagaccgat atgcttgcct cgcttatccc cggtgattgg gttacaagag accaattgga
      481 atacttcttt gataaagcat tacaagcgga atccaccatc ttaagaagct tcatatttgt
      541 atcggacagg cttaatgtgg aggtatattc ctatgctccc gccatcaaaa gagagacgca
      601 gacggtagca aaagccgata tggataaatt ctacagaaat aatctttcaa agcatatgcg
      661 cggagtgctt gccgggcaat atatttcttt gaaaaatacc gataatgagt tccactccga
      721 tatgtttatt atcatgagac ctatcttttc acagtacgga gatgtccagg cggttctcac
      781 tgtttgcaaa ttcattgatg actattcagg cgaattaaga accatcaata atgctctggt
      841 acttaccatg ataggtgtat cattactgat gttatttccc atctacagga tgtccaaaag
      901 catcaccaaa cctcttctgg agataacaga tgtagcggtt gcattcggaa aaggagattt
      961 ttccgtcaaa gccgaccaat cctatcctgc agaattaggc caattatctc tgaccatgaa
     1021 tcacctgtcc tcacagttaa gcaagacaat aaccagttta acacgtgaga ccaatattct
     1081 gcagggagta ttgaactcca tgaaccaggg tattttgctt gtggacgaga cactttctcc
     1141 catgattatc aatcccgctc ttccgaaatt ctttaaatcc acaatggata taaatgaaaa
     1201 acacagcatt attcccttga aagcggtatg ggaggatttt gagcaatgct tatcggagca
     1261 acagtatatt gagaatatat atgagtatga cggaaaaatg attctgtgcg ttataaatcc
     1321 cttaaaggta aatgatgcgt tgataggtgc gatgggaata ttcagcgata taacaaaaga
     1381 gatacgactg gaacagacaa gaagagatta tgtggcaaat gtatcacacg aattaaaaac
     1441 tcctcttaca ggtgttatgg gattattggc tcctcttaaa gatggaatag ttaccgaccc
     1501 cgataagatt acaaagtatt atgcaattat gtctaacgaa ttacagcggc tgaacagact
     1561 gataaacgat ctgcttatgc tgtcccgtct gcagtcggca agcgaggcat tcgccatgga
     1621 taaagttaac ctttcggcaa taattgtaga taagattgag aattatcatt ttgcttatga
     1681 ggataataat ctggcaatag attttaccgg tccttcgcaa cctgtgcttg tatacggcaa
     1741 tgccgatcgt atagaacaga tactgaccat acttattgat aatgcgataa agtactcaca
     1801 aaactcctcg gataagacaa taattaatga gaaaattaag ataaaaatag caatagtcaa
     1861 aaaagacgat aaatatatcg tttcggtgca ggattacgga accggaatat ccaaagagga
     1921 tatcgaatat gtgtttgaac gattctataa atcggacaaa tcccgttcgg gtgccggttc
     1981 gggcttggga ctggccattg cccgtgagac tctgaaaaga atgaacgaag atatatgggt
     2041 ggaaagtgac gggaaatccg gatcggtttt ctattttacc cttaccgagt ataaaccgaa
     2101 tattgaccga gttaagtgaa actacattaa attttaaccg ctccgtattt gcataattca
     2161 aaacctctga tgcctgctat tccgcaatat gctcttaaag cactgtccag acacacttta
     2221 tcatcaatat ggaatataaa aggctttttc agtatatcct gtatctgatt tataagttca
     2281 aacagaaatt gtgcattatt ttcgctgtaa aatatattaa acgcatcaca gggctcctcg
     2341 tttatgtcat aagcagcatc aatattgtcg tatacatcac cgtccgaaac agcaaaatca
     2401 acggtttcat aagagtcatc aacatcaaca tatccgtact gacagaagat atatcttttt
     2461 ttgtcggatg gcacggtaat gcttctgtct ttatctttga tgtaattcat ggctttgata
     2521 tattccggag ttgtaccgca acatccgccg attatcccca cattgaaatc cagtaaatta
     2581 tccgaatagc cgcaaaactc tatttttccc tggtcataat gagcaattcc gtccacaaag
     2641 ctcggaactc ccgcattggg ttttacacag acaaaatcct caaataatgc aatgtctttg
     2701 agtatatcgc ccattttatc cggtccgttg gaacagttta ctcccaccac atcggctccc
     2761 attttcttca gaataccggc acataccttc ggtgaatgtc ccataagagt catgttattc
     2821 ttctcaaatg tcatattgca tattacaggc agatttaccg tttctctgtt tgcaagataa
     2881 gcgattctca tctcttttaa atcggtaaaa gtttcaaagt taattacatc ggcagcacga
     2941 catgcatata tctggtcttt atacaaactg tatgcatatt cgaaagttaa cggtccgaaa
     3001 ggctgtaaaa gctctccgat aggtcctatc gatgccgcaa ttaatatgtt tcttccgtca
     3061 atcgcctgtt ttgcaagctc aataccctga ctgtttatct tgtcatgtaa ctcttccaaa
     3121 ccgtgacgct ttaatacaag cctgttggaa cataacgtat tggtctgaat tatgtcgctt
     3181 ccggcattgc agtattcttt atatatgcgc ataaccgtat ccggatgtgt tatattatac
     3241 gattccgcca gctctccttt ttgcaaaccg tatttttgca gcatgaatcc tttggaaccg
     3301 tcaaacagat atatcttttt tttgttaaat agtctcattt cttacacttt ttcctttagt
     3361 cccatttaat cttatcttta tattctgaag agttatatga gttgatgcaa ccgcagtatt
     3421 tctggcggta taacccgtac tcctttgctt tattctgacc tatcctgtaa ccttcccgaa
     3481 agtcatgata aaaaaactct atgccgtatt tatcggcaac actttccgct atgtcgataa
     3541 taagttgatg attctgataa atgcttacca gtaatgtggt ggaaaaccct ttataaccgt
     3601 tatccttggc atattttgcc gttttgtcca accttatact ataacaatcc ttacattgga
     3661 aaccctcttc ccaaagatgc tgcaaaaact catcatcttc aataagagga atgttaaaac
     3721 gtgacatata acttcttagc gttaaaagcc ttctctcatg ttcttttatc ggatgtatgt
     3781 tggggttata gaaatatgag tcataatcca cattcatttc attaagcttt tcatgaacat
     3841 atacaagaca aggtgcacag catgtatgta aaagtatttt atttcggttc ataaggtatt
     3901 ctcagatgct catatgcatg tgatgttacg catcgtcctt tcggcgtttt ctttataaag
     3961 cccaactgca gaagatacgg ttcatataca tcctcaatag tatctttttc ttcatttatt
     4021 gaagctgcaa gtgtttccag gcctaccggt ccgccgttaa acttggttat tatagtttcc
     4081 aggatagctc tgtctatatt atccaatcct attttgtcaa tttccagttt ttgcagtccg
     4141 taatcggcaa tttcacttgt aattacatct tctccttcca cttgtgcaaa gtcccttatt
     4201 cttcttaaaa gcctgtttgc tattctgggg gttcctctgg agcgggaagc tatctcattt
     4261 gctccgtatt cgttgatatt gaaccctaaa ataccggcac ttctctttac tatcttgctt
     4321 aattcctcta cggtatacat ctccagtctg caggatacac cgaatctgtc ccgcagcggt
     4381 gaggatagca tacccgtctt agtagtagct ccgataagag taaatttatt taaatccagg
     4441 cgtattgttt tggcactggg accttttccg ataataatat ccaaagcaaa atcttccatt
     4501 gcgggataga gtatttcttc taccgtacgg ttaagcctgt gtatttcatc aatgaacaga
     4561 atatcgttct cctcaatatt ggttaatatg gcggcaagat cccccgcttt ttctatagcc
     4621 ggtccgctgg ttatcctgat atttgctccc atttccgatg ctatgatacc cgccaaagtg
     4681 gtttttccta agcccggagg tcctgcaaga agcacatgat caataggttc acgccgtaat
     4741 ttggcagcct ctatgaaaac cttaagattt tcctttattt tttcctgacc gaaatactcg
     4801 tcaaaaacct taggccgtaa actcgcttcg ctttggtcgt tctttataaa tgacggagtt
     4861 acaaatcttt cttcctccat tacccttctc ctttatatct gctccgctaa agccttcttg
     4921 attatatcct gcaacgaatc ggactcatcg tataccttat tcagagcttc tcttgcatac
     4981 tttgtattat accccaatac aagcagtgca ttaaccgctt cggagtattt atcgctgacc
     5041 gcaatctgtt taaccgcatc ggcttcggca gcatagagac cttttaattt atccttaagc
     5101 tccagtatta ttctcattgc agtctttttt cccacaccgg gagctttgga taacgatgcc
     5161 gcatcttccg atactacagc tgtagccact tc
//
LOCUS       DAOPWC010000059         5029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_16740_length_5029_cov_3.471452, whole genome shotgun sequence.
ACCESSION   DAOPWC010000059 DAOPWC010000000
VERSION     DAOPWC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16740_length_5029_cov_3.471452"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PKJ65_02120"
     CDS             complement(<1..1831)
                     /locus_tag="PKJ65_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40668.1"
                     /translation="MTEDITHILTNKPVPEEIAKRLEKSADVQRLLFFIVGDLSKDSL
                     YSESVLAVTDKGMYCFDRTYDDGYKYVTHENIEKIEIKRLYGNAIMEISTEKEGTFDF
                     FRFTYSVANLCEAAADFSEQIKKGENVDRLMDMVTAVFEKQLSNCPRCGRTLVRPGAE
                     CLNCQSKGKIAKKLMKYVLPHKKLLIICLIFSIITTAMSLVPPYITKMMVDDIIPDNN
                     KKLLLTVVFVLFCTYVIQYSVGALRSYLLRVSGDRIVAGLRSDIYTKAQYLPMRFYDK
                     TSTGSVITRISSDTATLQSFTLRVTQEVIVQFFLLIGIIVIMFVMDRRLTLLSLIPVP
                     LVVIGSRIFGKKILPYYRRIWRKWSKVSSILTDTIPGVRVIKAFTNEKGAIDKFDKYN
                     EEWLKTDIKAARITTLFPQLVSFFVTCGSLLIWSVGGNLVMNPQSGLTLGLLVSFISY
                     TAMFYGPVNFFANFNDSLQAAVAAAERVFDIIDAEPEADFGKGNHPKKLEGKIQFKNV
                     NFSFDRTKMTLQDINIDIEPGDIVGIVGTTGSGKSTLINLIMRYYDNYDGKILMDGQD
                     IKDIDMEFYRSQIGYVQQEPMMFHDTIYNNIAYGNSDVQVEEVI"
     gene            2021..2767
                     /locus_tag="PKJ65_02125"
     CDS             2021..2767
                     /locus_tag="PKJ65_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ40669.1"
                     /translation="MSKYKCLIFDVDNTILQYDASAAKALEDLLLNHNVEYTPKIEEL
                     FRTVCYDLWKSYNMNLLDDPVMQEKYHEVYKIYGYKRFEVFLDKLNIGSLRKEHASDI
                     ADEYNEIFNNTHIFENHAIDVLKHCSKTHIVAAATNGIKEIQQSRLSPVRKYLSHLFI
                     SEEMGCVKPMKQFYNHMLETLNLSGNQCLFIGDLYTTDILGAINVQMDALWYINKERQ
                     SETAEWLESSNQYEITVPVIHSLLEIYRYI"
     gene            complement(2799..>5029)
                     /locus_tag="PKJ65_02130"
     CDS             complement(2799..>5029)
                     /locus_tag="PKJ65_02130"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40670.1"
                     /translation="FSIDSPLAQSKLFLRCNDEYITFTSQVGFGEGFAQDKMGRRIPL
                     VVNRNFFLCEDDYWSLAGLPVGYGYTDYSCTHTAGWSVIALKYKDISSKVRIFVPNTG
                     HMEIWSITLKNQSDRTRKLSLIAYAKTDIDAPYKPQGYNIARGSAFKDKNCVYGFFYT
                     QFNTERNIPIYGYMTSSESIKGYDSRRTAFIGAYNDDQRPKALEETKGCTNSDCMVEK
                     LCLALENSVTLKPGEEKTIHYVVGLAFGEDKIITPKDKWVQQQFTAMADKYEDRLRRI
                     KIKTPWENFDNLINGWMVYATDMGSRWARVRHNGYRDMCQDTDGLSVLNPELAWERIK
                     RVLSYQYENGYAPRTIIDGELKDRKFSDNTVWLTYTVYSCIKELNKPELLHEQVPFNN
                     GTSASVYEHIKRSVEFLYNFTGHYGLIRIWGGDWNDCINFAGLEGKGVSVWLSIAWCA
                     AAKQLCELAGMLGYEQDKKDYMEKYHIMRDRIDEHGWDGEYYMYARTDEDITMGSHTQ
                     EEGKIFLNPQIWAVFAGLDKDDRHIKSMDMAEKLLQSPEGILLSAPAYTRQYHYIGSM
                     AEKPGGVQDNGGVYLHPAAWKLAADSLLKRNDKVEENLKKMLPFDQTYYPTKGEPYCM
                     FNCYYLDYHGYRGGTPGQSWRTGTNAWLLQSTVKYIFGLAPEPEGLKLNPCLPPSWKE
                     CSITKLFRGCTYNIKYIQKGKGHNNRITRILMNGKETRFKDNTIPPVEGATLDIVVYL
                     EN"
BASE COUNT         1371 a         1142 c          972 g         1544 t
ORIGIN      
        1 ggataacttc ctctacctga acatcgctgt tgccgtaagc gatattgttg tatatcgtat
       61 cgtggaacat catgggttcc tgctgaacat atcctatctg agaccggtaa aattccatgt
      121 ctatatcttt tatgtcctgt ccgtccataa gaatcttacc gtcataatta tcataataac
      181 gcatgattag attgataagt gtggattttc ccgaaccggt agtacccaca atacctacaa
      241 tatctcccgg ttcaatatct atgtttatat cctgcaaggt catctttgtg cggtcaaaag
      301 agaaattaac attcttaaac tgtattttcc cttccagttt tttcggatga tttccctttc
      361 cgaaatccgc ttccggttcg gcatcgataa tatcaaaaac cctctccgcg gcggctacgg
      421 cagcctgcag agagtcgttg aagtttgcaa aaaagtttac cggtccgtag aacatggccg
      481 tgtaggaaat aaaggagacc agaagaccta atgtcaaccc gctttgggga ttcattacca
      541 gattgcctcc cacactccag ataagtaagg agccgcaggt tacgaaaaag cttaccagct
      601 gcggaaacaa agtggttatt cttgcggctt ttatatcggt cttcagccat tcttcgttgt
      661 atttatcaaa cttgtcaatg gctccctttt cgttggtaaa tgcctttata actctcactc
      721 cgggtatggt atcggtcaga atggaggaca cttttgacca tttcctccat atccttctgt
      781 aataaggcag tatcttcttt ccgaatattc tggaacctat tacaaccaga ggaacgggaa
      841 taagcgaaag caatgtaagc cttctgtcca tgacaaacat aatcactatt atccctataa
      901 gcagaaagaa ctgcactatt acttcctggg ttacccttaa ggtaaaggac tgcaatgtgg
      961 cggtatcgct gcttattctg gttatgaccg aacctgtaga tgtcttgtca tagaacctca
     1021 tgggaagata ctgagctttt gtataaatat cacttcttaa accggccact atcctgtctc
     1081 cgcttaccct taacagataa gaacgcaatg cacctaccga atactgtatg acataagtac
     1141 aaaacagcac aaataccacc gttaaaagca gctttttgtt attgtcgggt attatgtcat
     1201 ccaccatcat cttggttata tatggcggga ccaacgacat ggctgtggtt attatggaga
     1261 atatgaggca gattatgagc agcttcttat ggggaagcac atacttcata agttttttgg
     1321 ctattttacc tttggactga cagttaaggc attcggcacc gggacgcaca agagtccttc
     1381 cgcatctggg gcagttggaa agctgctttt cgaaaacggc tgtaaccata tccattaatc
     1441 tgtcaacatt ctcacctttt tttatctgct cggaaaaatc cgctgcggct tcacataaat
     1501 tggcaaccga ataagtaaaa cggaaaaagt cgaaagtgcc ttctttttct gtagatattt
     1561 ccattatggc attgccgtac agccttttta tttctatttt ttcaatattt tcatgagtta
     1621 catatttata tccgtcatca taggtcctgt caaaacagta catacccttg tcggtaaccg
     1681 caagaaccga ttcgctgtat aagctgtctt tggacaggtc tccgacaata aaaaaaagca
     1741 ggcgctgcac atccgccgac ttttcaagtc tttttgctat ctcttccggt acaggtttgt
     1801 tggttaatat atgagtgatg tcctctgtca tatttttacc tcctccaccc cttacccggt
     1861 tattatcggt tacccggcaa ttaaagtcaa catcaaaaac aacattttat ttctattgtt
     1921 aaaacaacat tgatttggta gaatagacaa aaataaaaat atcgcggcaa gaataatcat
     1981 tatattaacg ccaactgccg acgattgaac ggagcgcact ttgagtaaat ataaatgcct
     2041 tatatttgac gttgacaata caattttaca atatgacgca tcggcggcta aggcactgga
     2101 agatttgctt ttaaaccata atgtcgagta cacaccaaaa attgaagagt tgttcagaac
     2161 ggtctgttat gatttgtgga agtcttataa tatgaatctt ttagatgacc cggttatgca
     2221 ggaaaaatac catgaagttt ataaaatata cggctacaaa cgctttgaag tatttttgga
     2281 taaattgaat ataggaagtt taagaaaaga gcatgcttca gatattgccg atgagtataa
     2341 cgagatattc aataacactc atatatttga aaatcatgca attgatgttc tcaaacattg
     2401 ctcaaaaaca catatagtgg cggctgctac aaacggaata aaagaaatac agcaatcgcg
     2461 attgagtccg gtaagaaagt atctttccca tcttttcata tccgaggaaa tgggttgtgt
     2521 aaaacccatg aaacagttct acaaccatat gcttgaaaca ttgaatcttt ccggtaacca
     2581 atgtcttttc ataggagatt tgtataccac cgacatctta ggcgccataa acgtacagat
     2641 ggatgcccta tggtacataa ataaggaaag acaaagcgaa actgccgagt ggctggaatc
     2701 atccaatcag tatgaaataa ccgtacctgt catccattcg cttttggaaa tatatcggta
     2761 tatttagaat actataagca tacttatgtt gtatagggct aattttccag atacacaaca
     2821 atatccagtg ttgctccctc aacaggaggt attgtattat ccttaaaccg tgtttcctta
     2881 ccgttcatta atatccttgt tatcctgttg ttatgccctt tacctttttg tatgtacttt
     2941 atgttataag tgcatcctct gaacagtttg gttatggaac actctttcca ggaaggcggc
     3001 agacagggat tgagtttcaa gccttcgggc tcgggagcca agccgaaaat atacttgacc
     3061 gtagactgta aaagccatgc gttggttccc gttctccacg actgtccggg agttcctccc
     3121 ctgtatccgt gataatccag atagtagcag ttgaacatac agtaaggctc accttttgtc
     3181 gggtaatagg tctgatcgaa aggcagcatc tttttaaggt tttcttccac tttatcgttg
     3241 cgtttcaata acgagtcggc cgcaagtttc catgctgcgg gatgtaaata tacgcctccg
     3301 ttgtcctgta ctccgccggg tttttccgcc atggagccga tgtaatgata ctgtcttgta
     3361 taagcgggtg cggatagcag tattccttca ggggattgca acagtttttc cgccatatcc
     3421 atggacttta tatgcctgtc atctttatcc aatcccgcaa aaaccgccca tatctgcggg
     3481 tttaagaaaa tcttaccctc ttcctgagta tgtgatccca tggtaatatc ttcatcggtt
     3541 cttgcataca tgtagtattc tccgtcccag ccgtgctcat caatcctgtc acgcattata
     3601 tggtattttt ccatgtaatc ttttttatcc tgctcataac ccagcatgcc tgccagttca
     3661 caaagctgtt ttgcagccgc acaccatgcg atggaaagcc ataccgatac gcctttacct
     3721 tccagtccgg caaagttgat gcagtcattc cagtctccgc cccatatcct gataagcccg
     3781 taatgccctg taaagttgta taaaaactcc acacttcttt ttatgtgctc atataccgaa
     3841 gcggatgtgc cgttattgaa cggaacctgc tcatgcaaca gttccggctt gttaagctcc
     3901 tttatacagc tgtagactgt gtaggtcagc catacggtat tgtcggaaaa cttcctgtcc
     3961 ttcagctctc cgtctattat ggttctcgga gcatatccgt tttcatactg gtaggacagt
     4021 accctcttta ttctctccca tgccagttcg gggttgagaa cagacagacc gtcggtatcc
     4081 tgacacatat cacggtaacc gttatgtcgc acccgcgccc atctgcttcc catatcggtg
     4141 gcatatacca tccagccgtt tataagatta tcaaagttct cccagggagt ttttatctta
     4201 atccttctta agcggtcctc atatttgtca gccatggcgg taaattgctg ctgcacccat
     4261 ttatccttcg gtgttattat tttatcctca ccgaaagcca gacccaccac ataatgaatt
     4321 gttttttcct caccgggctt aagtgtaacg gagttttcca aggccaggca taacttctcc
     4381 accatacaat cactgtttgt gcagcctttg gtctcttcca aagctttggg tctctggtca
     4441 tcgttatatg cccctataaa agccgtcctt ctcgaatcat acccttttat gctctccgag
     4501 gaagtcatat agccgtatat gggaatgttt ctttcggtat tgaattgtgt atagaaaaat
     4561 ccgtacacac agtttttatc tttgaaggca cttccccttg ctatgttata tccctgcggt
     4621 ttatacggag cgtcaatatc ggtctttgca tatgcaataa gcgacagttt tcttgtcctg
     4681 tccgattgat tcttcaaggt tatgctccat atttccatat gaccggtgtt tggaacaaat
     4741 atcctgacct ttgaggatat atccttgtat ttaagagcta ttaccgacca tcccgccgta
     4801 tgagtgcagg agtaatcggt atatccgtat ccgacaggaa gaccggccaa tgaccagtaa
     4861 tcgtcctcac acagaaagaa gttacggtta acaaccaaag gtatacgtct tcccattttg
     4921 tcctgtgcaa atccttctcc gaatcctacc tgtgatgtga aagttatata ttcatcgtta
     4981 catctaagaa agagcttgga ctgggccaga ggactgtcga tggagaaac
//
LOCUS       DAOPWC010000060         5012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_16805_length_5012_cov_4.062538, whole genome shotgun sequence.
ACCESSION   DAOPWC010000060 DAOPWC010000000
VERSION     DAOPWC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16805_length_5012_cov_4.062538"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..600
                     /locus_tag="PKJ65_02135"
     CDS             400..600
                     /locus_tag="PKJ65_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40671.1"
                     /translation="MKVKIVDIKEKITDRKVMYKGRILEVEKVTALLPDGKEVYRDVI
                     RHSGACAIVALDEDMNIILEKQ"
     gene            625..951
                     /locus_tag="PKJ65_02140"
     CDS             625..951
                     /locus_tag="PKJ65_02140"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ40672.1"
                     /translation="MELPAGKKEDGEEPLQCAVRELEEETGYVADKMTHMITVASSPG
                     FSDEVVHIFLAEGLTRTQMKWDQEERLIVWKEPLSDAKKMVMEGKIKNGLAVAGILTA
                     CQLKGI"
     gene            951..1742
                     /locus_tag="PKJ65_02145"
     CDS             951..1742
                     /locus_tag="PKJ65_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ40673.1"
                     /translation="MIKFCPLFSGSSGNAIYLKYRDTSILIDAGLSKKKITQAIADIS
                     EDASELDAILITHEHYDHISGIGVMERGYNIPIYANTSTWKEMPRIFGRIREENKKTF
                     ETGKRFVIKDIEIMPFSIPHDTADPVGFSFFLDSKKITSVTDMGHLTETIAENIKGSD
                     LLLLESNHDLDMLINGRYPLKLKKRIQGEKGHLCNKDCGDIVVKFIEFGLKTVVLGHM
                     SAQNNVPQLAYDTVRKCIEENGYVIGKDINLVVAVREGNNRVFLL"
     gene            1739..2197
                     /gene="rlmH"
                     /locus_tag="PKJ65_02150"
     CDS             1739..2197
                     /gene="rlmH"
                     /locus_tag="PKJ65_02150"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNZ40674.1"
                     /translation="MKINIICVGKLKEKYLIEAQDEYVKRLSRFCDVKITEVSDEKDD
                     IPDAMEKEGARILSKIQPKDYVICLAKEGMKFDSEAFSEKVISVFDLSNPVIDFVIGG
                     SLGLSDSVKKRADMLLSFSDMTFPHRLMRVILLEQLFRAFKIANNESYHK"
     gene            2214..2351
                     /locus_tag="PKJ65_02155"
     CDS             2214..2351
                     /locus_tag="PKJ65_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40675.1"
                     /translation="MCKQRLQNQKDESKVFEDLLSASESSMDFWFNDVDDEQWNDEKN
                     D"
     gene            complement(2465..2986)
                     /locus_tag="PKJ65_02160"
     CDS             complement(2465..2986)
                     /locus_tag="PKJ65_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ40676.1"
                     /translation="MKAVTSHEFVLESLTSALIGLMKTKSFDKITVTELCEKACVGRV
                     SFYRNYNSKQDILVKYLAACTNEWWQEFSQKPEKEFYENFWPSLLDEFKKQKELLLLL
                     NQNNLNYLLRDHIFYCCGPSPDEDDNVAYIRALLAGMISGLIEEWVHRGMNDLPESLN
                     IHSMLKEALRSHE"
     gene            3138..4619
                     /locus_tag="PKJ65_02165"
     CDS             3138..4619
                     /locus_tag="PKJ65_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40677.1"
                     /translation="MKSKESKPKIDKSLWLTTKSERINYYVGDLGRTLEGYIVTAFMT
                     LFLLFQGINLISISAAILVVKVIDALDDVIFGFLIDKLNIKKSRLFGKIAGEGKYLPW
                     YRATFFLFPIFTVLFFLMPQGVPNAVKIGWFMVTYLLYDISYTLVEVPMNSMIVSLTD
                     NTEERNSILQTKGIIGGIATVLAGIIWTFLISEHVGISIRLVAIVSSVIFFFMMLPLA
                     TKVKEHNTELKNTEDEKSTERYNFRDMIKCVKTNKYMAVILLSTLIMGALATGSSLGT
                     YASYYLYGDSMILIIPIAIAFIPQLIAQLNTDKLCRKFGKKKVYMVCGLTAALIYGSI
                     FFAGYNNFVVITILLVLQAAPGNVANIAKTFFTPDTIEYTRYKTGKDCSGIFFSLNAF
                     VNKLTTSIASSLGVFILGLSEWITVEADSFEELAELNVVQTQSALDTLWIVFMLIPAL
                     GTLLSVVVMAFYKLKDDDAKLMAQCNAGEISREECETRLSRKY"
     gene            4638..>5012
                     /locus_tag="PKJ65_02170"
     CDS             4638..>5012
                     /locus_tag="PKJ65_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40678.1"
                     /translation="MLERDNVRFFDMTSVSPTQGIERRATGQYVKAGLARLFPGEVFR
                     NICVSFNGSGEYHPKVSLIQRMFGGGKKIEGLPAYYEVELEHLTGNHTETLIVWSPAA
                     WNDRFAGTAGGGTGIGGRSYLTK"
BASE COUNT         1555 a          930 c         1172 g         1355 t
ORIGIN      
        1 ataaagaaga atgccacccc tataagaaag ctgttcttta tctttacctc actgataagg
       61 aagaagtcac cgaatatgga aaagaaaaga cctgcgcata aaaacagcgc ggttgcagtg
      121 taattgccgg taaggatgac cgacacaacg gctgtagcaa aaaacataat gctcggagcc
      181 gtcttggtta tcttctcacg aggattttct ttgaatataa tgtgatacag ataaacacac
      241 aacgttatga aaaaaattgc caaacaaata aatacacctg tcatattaac ctctacccaa
      301 atagatttta acctatacag gcatataaat gcaaatacta accaaactat tttatataca
      361 caatcattta tataatcttt tagatggtat aatgtatcgg tgaaggtaaa gattgtggat
      421 ataaaagaga aaataacgga tagaaaagtc atgtataaag ggagaattct ggaagtggaa
      481 aaagtaaccg cacttcttcc cgacggaaag gaagtgtacc gggatgtgat tcggcacagc
      541 ggtgcctgtg ccatagtggc gctggacgaa gatatgaaca tcatactgga gaagcagtaa
      601 aggattgatt ttgacgatat tctcattgaa ttacctgcag gaaaaaaaga agatggcgaa
      661 gagcctttgc agtgcgctgt aagagaactg gaagaagaaa ccggttatgt agccgataaa
      721 atgactcata tgattacggt agcatcatca ccgggttttt ccgatgaagt ggtgcatata
      781 tttttagcgg aaggtcttac ccggacacaa atgaaatggg atcaggaaga gcggttgata
      841 gtatggaaag aaccgctatc cgatgctaaa aaaatggtta tggaaggcaa gataaaaaac
      901 ggactggcgg ttgcaggtat attgactgca tgtcagctaa aggggatata atgataaaat
      961 tttgtccttt gttcagcgga agcagcggga atgctattta tcttaaatat agagacacat
     1021 cgatactgat agatgcgggg ttgtctaaaa aaaagattac tcaggctata gcggatatca
     1081 gtgaagatgc atcggaattg gatgcaatat taataacgca tgaacactac gaccatatat
     1141 ccggtatagg cgttatggaa agaggatata acataccgat atatgcaaat acctctacgt
     1201 ggaaagaaat gccgagaata ttcggaagaa taagagagga aaacaagaaa acatttgaaa
     1261 cgggaaagag atttgttatt aaagatatag agataatgcc tttttccata ccgcatgata
     1321 ctgccgatcc ggtaggattc agtttctttt tagactcaaa gaaaatcaca tcagtgacgg
     1381 atatggggca tcttactgaa accatagcgg aaaacatcaa aggcagcgac ctgctgttat
     1441 tggagtccaa tcatgatttg gatatgctca taaacggtcg ctatccgctg aagttgaaaa
     1501 aaagaataca gggagaaaaa gggcatttat gtaacaagga ctgcggagat atagttgtta
     1561 agttcataga gttcgggcta aagaccgttg tgctcggaca tatgagtgca cagaacaatg
     1621 tgcctcagct ggcatatgat accgtaagga agtgcataga agagaacggt tatgtaatcg
     1681 gcaaagacat aaatctggtt gtagcggtaa gggaaggcaa taacagagtg tttttattat
     1741 gaagataaac ataatctgtg tgggaaaatt aaaagaaaaa tatctaattg aagcacaaga
     1801 cgagtatgtt aaaagactgt caaggttttg tgatgtgaag ataacggaag taagtgatga
     1861 aaaagacgat attcccgatg ccatggaaaa agaaggagca agaatactgt caaaaatcca
     1921 acccaaagac tatgtgatat gtcttgcgaa agaaggcatg aaatttgaca gtgaagcttt
     1981 ttccgagaaa gtcatatctg tattcgattt atcaaacccc gttattgatt tcgtgatagg
     2041 aggaagtctc ggtctttcgg acagtgtaaa gaaaagagcg gatatgcttc tgtccttttc
     2101 cgacatgact tttcctcacc ggttgatgag agtaatattg ttagaacagc tattcagagc
     2161 attcaagata gcaaacaatg agagttatca taagtagaag gatttaaaaa aaaatgtgta
     2221 aacaaagact gcagaatcaa aaggatgaga gcaaagtatt tgaagactta ttatcagcaa
     2281 gtgaaagcag tatggatttc tggtttaatg atgtagatga tgagcagtgg aatgatgaga
     2341 aaaacgatta aataactatt tacacttctt gagatacggt ttgtcaaggg gattttttac
     2401 cccttacatt aaaacgcaag aagtatgcag ttgagtattt cggttcaatc tgactccaat
     2461 ggtttcattc atgtgatctc aacgcttcct tcagcattga atggatgttt aatgattccg
     2521 gcagatcatt catgccgcga tgcacccatt cttctataag tccggagatc atacctgcga
     2581 gaagagcccg tatataggcc acattgtcat cttcatctgg tgaggggcca cagcaataga
     2641 atatgtgatc acgtaataaa taattcaagt tattctgatt aagaaggagc agcagttcct
     2701 tctgtttttt aaattcatcc aacagtgacg gccagaagtt ttcatagaat tccttttccg
     2761 gcttttggga aaactcctgc caccattcat tggtacaggc ggcgagatac ttaacaagaa
     2821 tatcctgttt ggagttgtag tttctataaa aagaaactct gccgacacag gctttttcac
     2881 aaagctccgt aacggtgatt ttgtcaaatg acttagtttt catgaggcca atcaaggcac
     2941 tggtaaggct ttcaagtaca aattcgtgtg atgttactgc tttcatagtg atacaaattc
     3001 cccgttttgt ttcttttttt gacgaagcaa tcctttctct tgatttcagg aaaaaggatt
     3061 gatacacttg caccatgaaa caaatgtatc agttggctgg gagcttgtca agcataatat
     3121 aatatcggga ggtttctatg aaaagcaagg aaagcaaacc aaagatcgat aagtctttat
     3181 ggcttacaac aaaaagcgaa agaatcaact actatgttgg cgatttgggg cgcacattgg
     3241 agggctatat cgtcacggcg tttatgacgc tctttctgtt gtttcaggga atcaacctga
     3301 tttcgatttc cgcggcaatc ctggttgtca aggtcatcga tgcgctcgac gatgtaattt
     3361 tcggtttctt gatcgacaaa ctcaatatca aaaagagccg actttttgga aaaatagccg
     3421 gtgaaggcaa atacctacct tggtaccgcg cgaccttctt cctgttcccg atttttacgg
     3481 tccttttctt tcttatgccg cagggcgtgc caaacgccgt gaagatcggt tggtttatgg
     3541 tgacctatct tctgtatgat atcagttata cgcttgttga ggtccccatg aactcaatga
     3601 ttgtgtcgct gacggacaac accgaggagc gtaactcaat ccttcagacg aaaggcatca
     3661 tcggcggcat tgcgacggtt ttagccggca tcatctggac attccttatt agcgaacatg
     3721 taggaatctc cataagactg gttgcgattg tttcgtctgt cattttcttc ttcatgatgc
     3781 taccgctggc gacaaaagtg aaggaacaca ataccgaact gaaaaacacc gaggacgaaa
     3841 agagcacgga gcgttacaat ttccgtgata tgattaaatg cgtaaagaca aacaaataca
     3901 tggctgtcat ccttctcagc acgcttatta tgggggctct ggcgacgggc agttctctcg
     3961 gaacctatgc gtcatactat ctgtacggtg actcgatgat tcttatcatt ccaatcgcga
     4021 ttgcctttat ccctcagctg attgcccagc tcaataccga taagttatgc cgcaaattcg
     4081 gaaagaaaaa ggtttatatg gtctgcgggt tgaccgcagc tctaatctat ggttctattt
     4141 tctttgccgg ttacaacaat tttgtcgtta ttacaatcct gctggtgctg caggcagcgc
     4201 cgggcaatgt tgccaatatc gcaaagactt ttttcactcc ggatacgatt gagtatacgc
     4261 gctataagac cggaaaagac tgctcgggga ttttcttctc cctcaatgcg ttcgttaaca
     4321 agttaacgac aagcatcgct tcttctttgg gcgtgttcat cctggggctt tccgaatgga
     4381 ttactgttga ggcagacagc tttgaggagc ttgccgagct caatgtagtg cagacccaga
     4441 gtgcactgga cactctttgg attgtcttta tgctgattcc cgcacttggc acactgctga
     4501 gcgttgtggt tatggcattc tataagctga aggatgacga cgcaaaactt atggctcaat
     4561 gtaatgccgg tgagattagc cgtgaggaat gcgagaccag attgtcgcgg aaatactgaa
     4621 aaacaggaga ctgtatcatg cttgaaagag acaacgtcag attctttgat atgacttctg
     4681 tctctccgac gcagggaatc gaacggaggg caacgggtca gtatgtcaaa gccggccttg
     4741 cacggctgtt tcccggtgag gttttcagaa atatatgtgt cagttttaac gggagcgggg
     4801 agtatcatcc aaaggtttct ctgattcagc gtatgttcgg cggcggaaaa aagattgaag
     4861 gcctacccgc atattatgaa gtggaactgg agcatctgac cggtaaccat actgagacgc
     4921 tcattgtttg gtcgcctgca gcctggaacg atcgttttgc gggaaccgcc ggaggaggga
     4981 ccggaatcgg aggacgaagt tatctgacaa ag
//
LOCUS       DAOPWC010000061         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_17023_length_4964_cov_3.524139, whole genome shotgun sequence.
ACCESSION   DAOPWC010000061 DAOPWC010000000
VERSION     DAOPWC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17023_length_4964_cov_3.524139"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKJ65_02175"
     CDS             complement(<1..1109)
                     /locus_tag="PKJ65_02175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ40679.1"
                     /translation="MYKTDNNRSKRKNTFKKKEHYKKKRKYDKQAGQPTKFRKRPVEY
                     NQDSRVLNYSGTGFADLHLSEGIMKALNKKGYVYSSTVQSAVIPPLMQYRDVIAKAPT
                     GTGKTFAFGIPLLEQLDNENKDIQALILAPTRELVIQICDELKELSEFLPKVRIEAIY
                     GGKAIEGQIKALKRHPQIIVATPGRLRDHIERKTIDISKVRTAVLDEADRMLDMGFIE
                     EVTGILNMMPDRKNLAMLSATMSIGVMDISWLYQRNPVEIAVEEQNEDKPDINQMYVT
                     VQNMRFKARAVREILDNNELYKVLIFCNTIRMVRMLTDDLREKGYSANCIYGGKSQAG
                     REKTIRAFKEERLNVLVATDVAARGLDITDVDAVI"
     gene            complement(1111..1720)
                     /gene="hisIE"
                     /locus_tag="PKJ65_02180"
                     /pseudo
     CDS             complement(1111..1720)
                     /gene="hisIE"
                     /locus_tag="PKJ65_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            complement(1701..2450)
                     /locus_tag="PKJ65_02185"
     CDS             complement(1701..2450)
                     /locus_tag="PKJ65_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ40680.1"
                     /translation="MPKAALQLYSIKDIAEQDFYHAMHIAKQVGFDGVEFAGFYGKEA
                     IETKKYLDDLGLLPAGSHSGLNAVVDKFHETMEYNSVIGNKYVAVPYLSEQFHSADGM
                     KTVAEYLNIACENAKKYGIKIGYHNHAFEFTVIDEGKRLFDILAENTVSDIFLQIDTL
                     WAVKGGVDVLEYCKKYKDRLDTLHFKDISEEGKDTELGRGLIDFVSILNVSDVIDWCI
                     LEQEQFDMPKEKSIAVSCAYLKGILDAKVAV"
     gene            complement(2515..3231)
                     /locus_tag="PKJ65_02190"
     CDS             complement(2515..3231)
                     /locus_tag="PKJ65_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ40681.1"
                     /translation="MDRKKVRNREDLFFIEGRKLLDEALSSRIRITEVYVSDTYYQNN
                     MSYVDKLTVKDVYLVRDGILESLSYMKSPEGILALGIPPKFDVPDVLKKGNPVLVMDR
                     INDPKNAGTMIRSAEAFGMGCVFALEGCTDLYNEKSLRASMGSIFRVPYIKISEEIFT
                     VLKNAGYVIYGLDANGHNIETVSLDKRCALVIGNESNGLDKKIRKHLTETLSIPMQGK
                     TESLNAACAAGIVMYKISLL"
     gene            complement(3288..3632)
                     /gene="rplT"
                     /locus_tag="PKJ65_02195"
     CDS             complement(3288..3632)
                     /gene="rplT"
                     /locus_tag="PKJ65_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ40682.1"
                     /translation="MARVKSGVTKRRRHKRVLKLAKGYFGQKSKNFRVAKQQVMKSLN
                     YAYIARRANKRNFRKLWIARINAAARTNGMTYSRFISALKNNGIELNRKVLADMAVND
                     PKAFASLVSSVK"
     gene            complement(3647..3844)
                     /gene="rpmI"
                     /locus_tag="PKJ65_02200"
     CDS             complement(3647..3844)
                     /gene="rpmI"
                     /locus_tag="PKJ65_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNZ40683.1"
                     /translation="MAKLKTHSASKKRFRVTASGKIKMGHAFRRHRLISKTKRAKTDN
                     RATAFASDADAPTIKKLIPYK"
     gene            complement(3857..4387)
                     /gene="infC"
                     /locus_tag="PKJ65_02205"
     CDS             complement(3857..4387)
                     /gene="infC"
                     /locus_tag="PKJ65_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ40684.1"
                     /translation="MAAQKGTKSQYRTNRSITAEEVRLIGTDGTAIGIMSVDEALKHS
                     EAAGLDLVEISPNARPPVCRIMDYGKFMFEISKREKEAKKKQKTMELKEIWLKLGIDE
                     HDINFKIKNACKFLADGNKVKVSLSFRGREMTRTQMGAEVLEKFTEACSEYGTPEKEP
                     KLEGRHMIVIIAPKKQ"
     gene            complement(4601..>4964)
                     /locus_tag="PKJ65_02210"
     CDS             complement(4601..>4964)
                     /locus_tag="PKJ65_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40685.1"
                     /translation="NAAKRETYTVATAEHNPWRVVADYYNELIGLKYKEVDKEDYISF
                     ISPDKNSPYARWQLEYDRMFDRIIDNSKILNIAGMKQSELMPLREGLKRELAVLPADT
                     FTAQSNTSRNMDIFLEKR"
BASE COUNT         1161 a         1215 c          850 g         1738 t
ORIGIN      
        1 tttatcacgg catccacatc ggttatgtcc aatccccttg ctgcaacatc ggtggctacc
       61 agcacgttga gccgctcctc tttgaaagct cttatcgttt tttcccttcc ggcctgcgac
      121 ttgcctccgt atatgcaatt tgccgagtat cctttttcac gcaaatcatc ggtcagcatc
      181 ctgaccattc ttatagtgtt gcagaatatg agcactttat ataattcatt gttatccagt
      241 atctctctta cggctcttgc cttgaaacgc atattctgaa ccgttacata catctgatta
      301 atgtccggct tgtcctcgtt ctgctcttcc acggctatct ccaccggatt tctttgatat
      361 agccaggata tatccatcac tcctatggac atggtggctg ataacattgc taagtttttt
      421 ctgtcgggca tcatattcag aataccggtc acttcctcaa taaatcccat atccagcata
      481 cggtcggctt catccaatac tgcggttctg acttttgaaa tatcaatagt tttgcgttcg
      541 atatggtctc ttaatcttcc cggtgtggca acaataatct gcggatgcct tttcaatgcc
      601 tttatctgtc cttctatggc ttttccgccg tatatagcct ctatccgcac tttaggaaga
      661 aactccgaca actccttcaa ctcgtcgcaa atctgaataa ccaactctct tgtcggcgcc
      721 agtataaggg cctgtatgtc tttgttttca ttatccagct gttccagaag cggtataccg
      781 aatgcaaaag tctttcccgt tccggtaggt gcttttgcaa taacgtccct gtactgcata
      841 agtggaggaa taacagccga ctgaacggtt gaggaataaa catacccctt tttattcaac
      901 gctttcatta tcccttcgga taaatgcaaa tcggcaaatc cggtaccgga ataattcagt
      961 acccggctat cctgattata ttctacaggt cgttttctga atttagtcgg ttgtccggcc
     1021 tgcttatcgt attttctctt ttttttgtaa tgctcttttt tcttaaaagt attttttctt
     1081 ttgcttctat tattatcggt cttatacata ttaccttctg tttctcaatt catcctcaat
     1141 cttatcgaga gttatacctc tttccacaag caataccatc aggtggtata caaggtcgga
     1201 tacttcatat acaatttcct gctcagagcc gttttttgcc gctataatgg tttccgaagc
     1261 ttcttcacct atcttcttta aaatcttatc cagaccttta tcaaaaagat aacaggtata
     1321 cgatccttct ttaggattgc tcaatctgtc ggctattacc tgatataact cattcaatat
     1381 ctcattcatt tttctttatc tccccgtttt gctctatctg tctgtagaaa caggactgct
     1441 cgccggtatg gcatgcctgc cctttctgtt taacaagata aatcaatgca tcaccgtcgc
     1501 agtccaccag cactttaagt acctgttgag tgttgccgct ggtctcgcct tttacccatt
     1561 cgctgtttct cgaacgggaa aagtatgtcg cttttcctgt cttaagagtt ttgtcaacct
     1621 gttctttttt tgcatatgcc agcataagta cctgtttggt atcaatatcc tgacatataa
     1681 ccggaattaa tccgttttgg tcaaactgca actttagcat ccagaatccc ttttaagtat
     1741 gcacaactta ctgctatgct cttttcctta ggcatatcaa actgttcctg ctccagaata
     1801 caccagtcga taacatcgga aacattcaaa atagatacaa agtctatcaa tcctcttccc
     1861 agttcggtgt ctttaccttc ttcggatata tccttaaaat gcagcgtatc caatctgtct
     1921 ttatactttt tgcaatattc cagcacgtcc accccgcctt taaccgccca gagtgtatct
     1981 atctgaagaa aaatatcgct gacggtattt tccgcaagta tatcaaaaag cctttttcct
     2041 tcatcaataa cggtaaattc aaaggcatga ttgtgataac ctatctttat accgtatttc
     2101 tttgcgtttt cgcatgcaat gttcagatat tccgcaacgg ttttcattcc gtccgcggaa
     2161 tgaaattgtt ccgacaaata cgggactgca acatatttgt tgcctataac ggagttgtac
     2221 tccattgttt cgtgaaattt atccacaacg gcattcaaac cggaatggct tcctgccggc
     2281 agtaatccca gatcatccag gtatttcttt gtttcaatag cttccttgcc gtaaaaaccg
     2341 gcaaattcca ctccgtcaaa acctacctgc tttgcaatat gcatggcatg ataaaaatcc
     2401 tgttccgcga tatcttttat ggaatatagt tgtagagctg ctttcggcat tatttcctcc
     2461 ttttataatc ggcatctatc agcaattata cacattaacc gataccaatt caaatcacag
     2521 taacgatatc ttgtacatta ctatacctgc cgcacatgcc gcatttaacg attccgtctt
     2581 accttgcata ggaatggaca atgtttctgt aaggtgcttt cttatctttt tatccaaccc
     2641 gttggattca ttacctatga ccaatgcaca tcgtttatcc agactgaccg tctcgatatt
     2701 gtgtccgttt gcatccaatc cgtatataac atagccggca tttttcagca ctgtaaaaat
     2761 ctcttcgctt atctttatgt acggaaccct gaatatcgaa cccatgcttg ccctcaagga
     2821 tttttcgtta tataaatcgg tacatccttc cagtgcaaac acgcaaccca ttccgaaagc
     2881 ttcggcactt cttatcattg ttccggcgtt tttcggatcg tttattctgt ccattacaag
     2941 caccggattg ccttttttaa gcacatccgg aacatcgaat ttaggaggta ttcccagtgc
     3001 cagaattcct tcgggacttt tcatgtagga caatgactcc aatataccgt ctctcacaag
     3061 atagacatcc ttgacggtaa gcttatccac ataagacata ttgttctgat aatatgtgtc
     3121 gcttacatac acttccgtta ttcttatacg gctgcttaat gcttcatcca gcagtttcct
     3181 gccttcgatg aaaaaaaggt cctcacgatt gcggaccttt tttctatcca agcttttcag
     3241 ccttttgatt ttcttattgt ccccggattc tatgcggttc atggttttta cttaaccgaa
     3301 ctgacaagtg atgcaaaggc tttcggatcg tttaccgcca tgtcggcaag tacttttctg
     3361 ttaagttcaa taccgttatt cttgagagcc gatatgaatc tggaataagt cattccgttt
     3421 gttcttgccg cagcatttat tcttgcaatc cagagctttc tgaagttacg cttgtttgct
     3481 cttcttgcaa tgtatgcgta attcagcgat ttcattacct gctgcttggc aactctgaaa
     3541 ttcttactct tttgtccgaa atatcccttt gcgagcttta atactctttt atgtcttctg
     3601 cgttttgtta cgccgctttt tactcttgcc atttgtctgt tcctccttac ttgtacggga
     3661 taagcttctt gattgtcggt gcatccgcat ccgatgcgaa agccgttgct ctgttatccg
     3721 tttttgctct cttggtctta gatatcagtc tgtgccttct gaaagcatgt cccattttta
     3781 tctttccgct tgcggttact ctgaaccttt ttttcgaagc gctgtgtgtt tttagttttg
     3841 ccatattcta atcctcctat tgtttcttgg gcgcaattat aactatcata tgtctgcctt
     3901 ccagtttcgg ttctttttcc ggtgtgccgt attccgaaca ggcttccgta aacttctcca
     3961 atacttccgc tcccatctgc gttctggtca tttcacgtcc ccgaaatgac aggcttacct
     4021 ttactttgtt gccgtccgca aggaatttgc aggcattctt tatcttaaaa ttaatatcat
     4081 gctcgtctat tcccaatttc agccagattt ctttcagttc catggttttc tgtttctttt
     4141 tagcttcctt ttcccttttg gaaatttcaa acatgaattt gccatagtcc atgattctgc
     4201 atacgggcgg tctggcattg ggggaaatct cgactaaatc cagtcctgcc gcttcactgt
     4261 gcttgagcgc ttcgtcaacc gacattattc ctatcgccgt tccgtccgtt cctattaatc
     4321 tgacttcttc cgctgtaata ctcctgttag tcctgtactg gcttttggta cctttttgag
     4381 ctgctatcgt aattacctcc gtaaaaatta taatggtcct gcagacaata atccgcaaga
     4441 ccacacaaca agttaatgcg atatgtttac ccgttcagtc gttacttagg ggtgagaagg
     4501 gattacttct gctttccgtg cgttatacta tcatatggaa tataagttgt caaatataac
     4561 ttttcatttt ttatccgaca gtatacctta tattccgtat ctaccgtttt tccaggaata
     4621 tatccatgtt tcggcttgta ttgctctgcg cggtaaaggt atcggcagga agaacggcaa
     4681 gttccctttt caagccttca cgtaacggca taagctccga ctgcttcatc ccggctatat
     4741 tgagtatctt ggaattgtct atgatacggt cgaacatacg gtcatattca agctgccagc
     4801 gtgcataagg actgttttta tcgggtgata taaaggaaat atagtcttct ttgtcaacct
     4861 ccttatattt aagccctata agttcattgt aataatccgc aacaactctc cacggattat
     4921 gctcagccgt tgcaactgta tatgtctctc tttttgcagc gtta
//
LOCUS       DAOPWC010000062         4950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_17093_length_4950_cov_3.768335, whole genome shotgun sequence.
ACCESSION   DAOPWC010000062 DAOPWC010000000
VERSION     DAOPWC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17093_length_4950_cov_3.768335"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKJ65_02215"
     CDS             <1..831
                     /locus_tag="PKJ65_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNZ40686.1"
                     /translation="KDIFGKMIDALYTEGIYTGYRYYDKAGVPVRWPFGYGLSYTSFD
                     YANLNIKEKTVTVTVKNIGDRAGAEVVQLYIGAPQDGIHRPVRELKGFKKIFLQPGES
                     TTVSFELEDRSYSIWQNGWKIPGGTYTIHIADLSESIKISGESIDIPAWQKESWYQSC
                     KGKPTLKDWELLTGKTYTVPVLKKGKFTMNNTVMEMKDYSFVMRIMVKVVERIVAEVN
                     GCKVDYDNPDFRMMINTSLGAPLRSMQIAGGVKDGIMQGLLDMANGHFFRGIGKMIKG
                     "
     gene            846..2264
                     /locus_tag="PKJ65_02220"
     CDS             846..2264
                     /locus_tag="PKJ65_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40687.1"
                     /translation="MSKAETEERRIKRKAKELKELEKWKKEQARPKRKFYLLYLMVVL
                     CLVFIVDTVATEISVKMKAEICHYLFDDSTAVMDLLGMLASAVMIAGAIYKSMSDRFG
                     RKIFLVLNTVAMGLGMFIVYLAGEIGSAAVYVIGSLFITFFIAHDMQAVYVIETVPSK
                     YRFTILSIVTSVASLGSILIPVLRRNIMGNNTELWNKVFLIPAILGAVVGLIAMITAR
                     ETDVFLNQRIKFLEMTDEQREAEKEQRHAEAQAQGGLGAAFRFAFRHKQLRWIFICTM
                     VVSMAMIGTMYYQQIMTYGYEAAYGTSTPEVVTDALMVMPIIGAVFTLARGFISDIFG
                     RKTVAVVMASLAVVGMIGLYLGVTFVWNPVLVGLFTGIYVSGYWCAADAMGGNMSAES
                     SPTNLRASIIGAQSLCNMVGMGVSFPAMIAVAAIAGNNALGIACLFLVVIPLVVAIIL
                     FLAKVAETKGIDLNTVRGDEWD"
     gene            2398..4821
                     /locus_tag="PKJ65_02225"
     CDS             2398..4821
                     /locus_tag="PKJ65_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNZ40688.1"
                     /translation="MQDYEKLGVFYIGKKYDIENRKLQDEYVLMKSKNLTTHAAIIGM
                     TGSGKTGLGIGLIEEAVMDNIPVIAIDPKGDLTNLALTFPMLRAEDFRPWINLQDASN
                     KDMDPDSYAQAQADSWQKGLAEWGQDGLRIQRLKNESDVKIYTPGGSSGIGVSVLKSF
                     NAPSKEVLADKDTYRDRISATTTGLLTMLGIAADSFTSKEFILISKILDNSWSEGKSI
                     TLPKLIAEIQHPPFDKIGVMDVNSIYPSKDRFALAMLLNNLIAHPGFEAWLEGEPLDI
                     DRFFYSHSGKPKVSIFSISHLSDNERMFFVTMLLNEVLSWVRSQPGTGSLRAMIYMDE
                     LFGYLPPVANPPSKAPLLTLLKQARAFGLGLVLATQNPVDLDYKALSNAGTWFIGRLQ
                     TERDKLRVLEGLEGVALGGSFDKGRTEKILAGLGQRVFYMHSVYDNEPVIFNTRWAMS
                     YLAGPLTKDQIAKLPEQYITSDRLTVESHISQPDSAASITQRDLSTKPVLPPAIKQVF
                     LPEKTRARGKLIYKPAVLGVADAVYNSEKHMITATKSYTILTRFNEGPVTLDWNQSRI
                     IDTELGDIETTPIEGALFSDYPAAAADAKNYDTWKKLLSQYIRTNLTLKLYSSPKLKL
                     VSQYDEDERDFLIRVQHTAHERRDAALEAMKKKYSSKIQTLDNSIIRARQAVEKQNVM
                     ASQKKMDAMVSTGSAILGALLGRKTISATSVSRLGTAVSRTSKAMKSGQSIDQAEERL
                     KALEAQQETLQTELEQQLKDISYEYESVKDVIETVNIRATSTNITVHLLGLAWVPYEK
                     GELTDTILI"
BASE COUNT         1448 a          986 c         1225 g         1291 t
ORIGIN      
        1 aaggatattt tcggtaaaat gattgacgct ctgtatacag agggtatcta tacaggctat
       61 cggtattatg acaaagccgg tgttcccgtt cgttggccgt tcggttacgg tctcagttat
      121 acaagttttg attatgcaaa tctgaacata aaggaaaaaa ccgttacggt taccgttaaa
      181 aatatcggcg accgtgcggg agcggaggtt gtgcagttgt atatcggtgc tcctcaggac
      241 ggcatccatc gtccggttag ggaactgaaa ggtttcaaaa agatattttt acagcccggt
      301 gaaagtacga cggtttcctt tgaacttgaa gaccgcagct actcaatctg gcagaacggc
      361 tggaagattc ccggcggtac atacacaata cacattgcgg atttgtctga aagtattaaa
      421 atttcaggtg aaagtataga tattcccgca tggcagaaag aatcatggta tcaatcgtgc
      481 aaaggaaagc ctacattgaa agattgggag ttattgacgg gtaagaccta taccgttccg
      541 gtattgaaga aaggtaagtt tactatgaat aataccgtaa tggagatgaa ggattactcg
      601 tttgtaatga gaataatggt caaggttgtg gaacggatag ttgcagaagt caacggttgt
      661 aaagtggact atgacaatcc ggattttcga atgatgatta atacctcatt gggtgcaccg
      721 cttcgcagca tgcagattgc aggcggtgta aaagacggta ttatgcaggg acttttggat
      781 atggcaaacg gacacttttt ccggggaatc ggaaaaatga taaaaggata acagggggaa
      841 taattatgag taaagcggaa accgaagaaa gacgcattaa aagaaaagct aaagaactga
      901 aagaattgga aaagtggaaa aaagagcagg ctcgtccaaa acgtaaattc tatctgttgt
      961 atcttatggt agtgctgtgt ctggtgttca tagtggatac cgttgcaacc gaaataagtg
     1021 tcaaaatgaa ggcggaaata tgccactacc tgtttgacga ctctacggct gtcatggatt
     1081 tattaggtat gctggcttcg gcggttatga ttgcaggtgc gatatataag tccatgtccg
     1141 accggttcgg ccgaaagata ttcctggtgc tgaacaccgt tgccatgggc ttgggtatgt
     1201 ttattgtcta cttggcaggt gaaataggtt ccgctgccgt ttacgtcata ggttctctgt
     1261 ttatcacctt ctttattgcc catgatatgc aggcggtata tgtgatagaa accgtgcctt
     1321 ccaagtaccg atttaccata ctgtcgatag tcacttccgt tgccagtctg ggcagtattc
     1381 ttattccggt attgcgacgt aatataatgg gcaataatac tgaactttgg aataaagtat
     1441 ttcttatccc cgctatttta ggtgccgttg taggtctgat tgcaatgatt accgcacggg
     1501 aaaccgatgt tttccttaac caaagaataa agttcctgga aatgaccgat gagcagcgtg
     1561 aggcggaaaa agagcagcga catgcagagg ctcaggctca gggcggtttg ggggcggcat
     1621 tcagatttgc tttccgtcat aaacagttac gctggatatt catatgtaca atggtggttt
     1681 ccatggctat gataggtacc atgtactacc agcagattat gacctacggt tacgaggctg
     1741 cttacggcac atccaccccc gaggtggtta ccgatgcgct gatggttatg ccgataatcg
     1801 gtgcagtctt cacattggca agaggtttca tcagtgacat attcggaaga aagaccgttg
     1861 ccgttgtaat ggcctctttg gcggttgtag gcatgatcgg tttgtattta ggtgttacct
     1921 ttgtatggaa tccggtgctg gtgggactgt ttaccggtat atatgtttca ggatactggt
     1981 gtgcagccga tgccatggga ggcaatatgt ccgccgagtc ttctcccacc aacttacgtg
     2041 cttccatcat aggagctcag agtctgtgta acatggtagg catgggtgtt tccttcccag
     2101 ctatgattgc ggttgctgcg attgcgggga ataatgcttt gggtatagcc tgccttttcc
     2161 ttgtggtaat tccgctggtg gttgctatta tactgttctt agccaaggta gcggaaacca
     2221 aaggtatcga tctcaatacc gttcgcggag acgagtggga ctgacaatat attaaaatcg
     2281 gagtcactga gtgaacccga aaacatttaa taagtatata gtggctgaaa tggtatataa
     2341 tttaagtacc gattctatta acatatcatg atgtatggaa aaacggaggg atatataatg
     2401 caggattatg aaaagctcgg agtgttctat atagggaaaa aatatgatat agaaaaccgt
     2461 aagttgcagg atgaatatgt tcttatgaaa tcaaagaacc tgacaacgca tgccgccatt
     2521 attggaatga ccggaagcgg taaaacgggg ctaggaatag gacttattga agaggctgta
     2581 atggataata ttcccgttat agccattgat cccaaaggcg atctgacaaa ccttgctctg
     2641 acattcccca tgcttagagc ggaagatttc cgtccgtgga taaatcttca ggatgcttcc
     2701 aataaagata tggaccccga ctcttatgca caggctcagg ccgattcctg gcaaaaggga
     2761 ttggccgaat ggggtcagga cggtttaaga atacagcgat tgaaaaacga gtcggatgtg
     2821 aaaatatata ctccgggagg ctcttccggt ataggcgtat cggtattgaa gtctttcaat
     2881 gctccctcta aggaagttct agcggataaa gatacatatc gtgacagaat aagtgccacc
     2941 actacggggc ttctgactat gctgggaata gctgccgact cctttacaag taaagaattc
     3001 atactcatat ccaagatttt agacaacagc tggtcggaag gaaaaagcat aacgcttcca
     3061 aagcttattg cggaaattca gcatccgccg tttgataaaa taggcgttat ggatgtaaac
     3121 agcatatatc cgtcaaaaga ccgctttgca ttggctatgc tgttgaataa cttaatagca
     3181 caccccggat ttgaagcctg gctggaagga gaacctttag atatcgacag gttcttttac
     3241 agccattcgg gcaaacccaa agtgtcgata ttttccatat ctcatctttc ggacaatgag
     3301 aggatgttct ttgtcaccat gctgttgaat gaagtactgt cctgggtcag aagtcaaccg
     3361 ggcacaggaa gtttgcgggc tatgatatat atggatgaat tgttcggtta tcttcccccc
     3421 gttgccaatc cgccgtccaa agcaccttta ctcacacttc ttaaacaagc ccgtgctttc
     3481 ggcttaggcc tggtgcttgc aacacagaat cccgtggatc tggattacaa ggctctttcc
     3541 aatgccggaa catggtttat aggaaggttg cagaccgaga gagataaact aagagtactg
     3601 gaaggactgg aaggcgttgc attgggaggc agtttcgata aaggacgaac cgagaagatt
     3661 ttagcgggct tgggacaaag agtcttttac atgcacagtg tttacgacaa tgagccggta
     3721 atattcaata cccgatgggc gatgtcttat cttgcaggac cgctgacaaa agatcagatt
     3781 gcaaaactgc ccgagcagta tataacttcg gacaggttaa ccgtggagag tcatatttcc
     3841 caacccgatt cagctgcttc cataacacaa cgggatttat cgacaaaacc ggtattacct
     3901 cccgctatca agcaggtctt tcttcccgaa aaaacccgcg cccgtggcaa acttatctac
     3961 aaaccggcgg tattaggagt agcagatgcg gtgtataaca gtgaaaagca tatgataacc
     4021 gctaccaaat cctataccat actcacccgc ttcaacgaag gaccggtaac tttggactgg
     4081 aatcagtcac ggattataga tacggaactg ggcgatattg aaacaacccc gattgaagga
     4141 gcgcttttct ccgattaccc cgccgctgca gccgatgcaa agaattacga tacgtggaaa
     4201 aaattattgt cacagtatat acgcaccaac ttaacattga aactttattc gtctcccaaa
     4261 ctcaaactgg tttctcagta tgatgaagat gaaagggact tcctgataag ggtacagcat
     4321 accgctcatg aaaggcgcga tgccgctttg gaagccatga aaaagaaata ctcctccaaa
     4381 atacagactc tggataattc cattatacgg gcacgtcagg cggtggaaaa acagaatgta
     4441 atggcctccc agaaaaagat ggatgcaatg gtatccaccg gaagtgccat attgggagcc
     4501 cttctgggaa gaaaaaccat cagtgccact tcggtttccc gattgggtac ggcggtaagc
     4561 agaacatcca aagccatgaa gagcggacaa agtatagacc aggcggaaga aaggctgaaa
     4621 gccctggaag cacagcagga aacacttcag accgaactgg aacagcaact gaaagacata
     4681 tcttacgaat atgaaagcgt aaaggatgta atagaaaccg tcaatatccg ggcaacaagc
     4741 accaatatta ccgttcattt attaggtctt gcctgggttc cttatgagaa gggagaactt
     4801 accgatacaa tccttatata accgctttac cgttttaatt gcaaggtaaa gaaacggcgg
     4861 ttaaggtgta atataaagga aggaacagtc atggataact cgacagtatt ttgggtaata
     4921 tcgatatttg cggtaggtgc gctggtagtt
//
LOCUS       DAOPWC010000063         4901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_17326_length_4901_cov_3.111845, whole genome shotgun sequence.
ACCESSION   DAOPWC010000063 DAOPWC010000000
VERSION     DAOPWC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17326_length_4901_cov_3.111845"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKJ65_02230"
     CDS             complement(<1..1085)
                     /locus_tag="PKJ65_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ40689.1"
                     /translation="MLHLKEDERTYVSLDNPALRNLARFNPELFFERFKTPLLIDEIQ
                     YAPELFPYIKMIIDARNENNLFWLTGSQVYPLMRGVQESLAGRVRILQLDGFSVKEYK
                     EQEHNPFPRDLSDMMREDILSFSVAKTIIQGSMPRVLFQKNIDVAGYYQSYIDSYIAR
                     DVREITNVLDTGQFLHFMTLLAICTAQELNLASIGKDLGVDSSTVRRWLDVLLTSGLV
                     IELPAYSRNLGKRIIKRPKIHFCDVGLAAYLCSIRTVEAFDMNPLKGNLFESWIVSEI
                     YKSYRHNGISPQMYYYRDSNQREIDLLIEMGGKLYPFEIKLSDNPSHAEKNFSVLAQE
                     DLRIPYYGVICHTNTLSPVKEKIWKIPV"
     gene            1526..2062
                     /gene="pyrR"
                     /locus_tag="PKJ65_02235"
     CDS             1526..2062
                     /gene="pyrR"
                     /locus_tag="PKJ65_02235"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNZ40690.1"
                     /translation="MEFKATLMDEDAVRRALIRISHEILEDNKGPEGICLVGIKTRGI
                     PLSERIAKNIENISSVKVATGILDITLYRDDLSKYSEDPILNATDIQFEIKGKNVILV
                     DDVIFTGRTARAAMDAVFAIGRAAKIKLAVLIDRGHRELPIRPDFVGKNVPTAKDEMI
                     AVKLSETDGITGVCLYHI"
     gene            2098..3438
                     /locus_tag="PKJ65_02240"
     CDS             2098..3438
                     /locus_tag="PKJ65_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNZ40691.1"
                     /translation="MGKTKNEGFTLKTAILSFQHMFAMFGATILVPILTGMSISVALL
                     SAGLATIAFYFIAKKKVPVFLGSSFAFLPALISGMQGAGEIGSDTWNRAMGKVSVAVM
                     LAGLVYVIIALFLKRIGVDRIRKLFPPIVVGPVIVVIGMILAPKMFYNNIIGQAIWTG
                     VPAWKHWTAAGITALTILLVSAVAKPKSFLKVIPILMGFVVGYIYSLIIGLVDYSNVD
                     WSQIIIFQDMVKTFGFYKYLTFDWGVILSLVPIAVVTFMEHLGDIAASSTVCGKDFMK
                     DPGVHRTLMGDGVGTMIAGVLGGPPNTTYGENTAVLAITKNYEPKNLFYAGIMAVIFG
                     SITIFGTAVGTIPSAVIGGASIVLFGMISAAGLRALVDGKVDFSDTKNILVVSVILSI
                     GLGLGAMSLAGDVTGDPIYKIMIGTVEISPLAIATFAGIILNLVIPSDNREEKK"
     gene            3492..4676
                     /locus_tag="PKJ65_02245"
     CDS             3492..4676
                     /locus_tag="PKJ65_02245"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ40692.1"
                     /translation="MMLLKNAYIYKDGSFHASDLFIIDDKRWIAGKNLSAVKTDKVID
                     LQGKYIIPGLVDVHVHLRQPGFEFKETILTGSKSGAAGGFTSICAMPNVVPAPDSIDN
                     FRRIRKIIDSDACINVYQYGCITKKREGMELADIGKLTGYVIGFSDDGNPVSSESVMA
                     AMLEQASLHDCLIAAHCEDLSYEDPAKREYMHIERDIGLLRKTGISCRYHVCHVSRKE
                     SVELIRQAKKEGINITCETAPHYLIFTKEDVKSENYIMNPPLGNKEDREALLQAVCDG
                     TIDIIATDHAPHTKEDKAAGAMGVIGMETAFSSMYTYLVAAGRVSLERLVEFMSVNPA
                     RIFGIPQYSDFTVLDLDKEYIVNANEFFSMGRNTPFEGMRMKGRVIKTICKGKIVWEY
                     TQ"
     gene            4658..>4901
                     /locus_tag="PKJ65_02250"
     CDS             4658..>4901
                     /locus_tag="PKJ65_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNZ40693.1"
                     /translation="MGIYTIESIRQLTYDTYEMTLSGDMSWIKAPGQFVNIKIDGFFL
                     RRPLSVCDIDDRYMTLIFKVVGQGTKALSMMKVSSQL"
BASE COUNT         1491 a          885 c         1133 g         1392 t
ORIGIN      
        1 acgggaattt tccaaatttt ctctttaaca ggacttaatg tatttgtatg acatatcact
       61 ccgtaatagg ggatacgcaa atcttcttga gctaaaacgg agaaattctt ttctgcgtga
      121 gagggattat cgcttaattt aatttcaaac ggatacagtt ttcctcccat ttcaatgagt
      181 aaatctattt ctctttggtt actgtcgcgg taataataca tttgcgggct tatgccgtta
      241 tgtcgataag acttgtatat ttccgaaact atccaacttt caaaaagatt tccttttagc
      301 ggattcatat caaaagcctc gactgtcctg atagaacaca ggtaagcagc cagtccgaca
      361 tcacagaaat gaattttggg ccgtttaatg attctctttc ccaaatttcg gctatatgca
      421 ggcagctcga ttaccagacc gcttgttaag agaacatcca gccaccttct caccgtactg
      481 ctgtcaacac ctaaatcttt tccgatactt gccaagttta attcctgtgc ggtacatatt
      541 gcaagcaacg tcataaaatg caggaactga ccggtatcca aaacattggt aatttctctc
      601 acgtcacgtg cgatataact gtcaatgtat gactgatagt aacctgcaac atcaatattc
      661 ttttggaaaa gaactcttgg catggaacct tgtataatag tttttgcaac agaaaaagac
      721 aagatatctt cccgcatcat gtcggacaaa tcacggggaa aaggattatg ctcctgctct
      781 ttgtattctt ttacggaaaa tccgtctaat tgcaaaattc tgactcttcc cgccaacgac
      841 tcctgcaccc cgcgcatcag aggataaact tgcgagccgg taagccaaaa gagattgttc
      901 tcattacggg catctataat catttttata tacggaaaaa gttcgggagc atattgaatc
      961 tcatcaatca gaagaggagt tttaaagcgt tcgaaaaaca attcgggatt gaatcgtgcc
     1021 agatttctca aagcgggatt atcaagactt acatatgttc tttcatcttc tttcaaatgt
     1081 agcaaaagcg tgcttttacc cacctgtcgt ggtcctgtaa gcaaaagtgc aggataatat
     1141 tgtgtttgct atttaaaata ctcttcaatt tggcgtctgc gataagtaat cattttgata
     1201 tacctctcta taattaagat tattatacta aacatattca tgaaaatcaa ggattcaatc
     1261 cctgaaaaac ggggatgcaa tccttgaaaa gcataaattc ctgttaaaga caggaacttt
     1321 atccaaacat aagtgaggta ttttttgcgt aaatttgtgc ggcaaattgc taaaaccctc
     1381 ttgatatatt tgagcattaa tggtactata ctccaagaac tttaagtgcg gtgcagagat
     1441 acccacaagg tgtttataaa gaacaggttt gtctatcctt gtatgcattg cgcaacaggg
     1501 atttttttta gataaggagg gggacgtgga gtttaaagcc actttgatgg atgaagatgc
     1561 ggtaagaaga gcacttattc gtatttcgca tgagatactg gaagataaca aaggtccgga
     1621 aggtatctgt cttgtaggca ttaaaacaag gggaatccct ctatcggaaa gaattgcaaa
     1681 gaatattgaa aacatttcct cggttaaagt tgcgacaggt atattggata ttacccttta
     1741 tcgtgatgat ttatccaaat attccgaaga tcctatactc aatgcgacag atatacaatt
     1801 tgaaataaaa gggaaaaacg tcatcctcgt agatgacgtt atttttaccg gtcgcaccgc
     1861 acgtgccgca atggatgctg tttttgctat aggcagagcg gctaaaatta aattggctgt
     1921 gcttattgac agggggcaca gagagttgcc tataagaccg gattttgtgg gaaagaatgt
     1981 tcctacggcc aaggacgaga tgatagcagt aaaattgtcg gaaaccgacg ggataacagg
     2041 cgtatgcctt tatcatatat aaaagtagcg gaaataaatc aggaggagaa aaaaactatg
     2101 ggaaaaacaa aaaatgaagg cttcacatta aagacggcta tactgagttt tcagcatatg
     2161 ttcgcaatgt tcggagcaac aatactcgta cctatcttga cgggaatgag tatatcggta
     2221 gcactgcttt cggcgggatt ggcaacaata gcattctact ttatagctaa gaagaaagtt
     2281 ccggtatttt tggggagcag ttttgcattt cttccggctc ttatatccgg aatgcagggt
     2341 gccggtgaga taggatcgga tacatggaac agagcaatgg gaaaagtaag cgttgccgta
     2401 atgctggcag gtcttgttta cgtcataatt gctcttttct tgaagagaat aggcgttgac
     2461 aggataagga aattattccc gccgatagtg gtaggtcccg ttatagtggt tataggtatg
     2521 attcttgcac ccaagatgtt ctataacaac attatagggc aggctatttg gaccggagtt
     2581 cccgcctgga agcactggac ggcagccggt atcaccgctt tgacaattct tcttgtaagt
     2641 gcggttgcaa aacccaagag ctttttgaag gtaataccta tacttatggg ctttgttgtc
     2701 ggatatatct attcactcat aatcggtctt gtggattatt ccaatgtcga ctggagccag
     2761 attatcatat tccaggatat ggtaaaaaca ttcggtttct ataagtacct gacttttgac
     2821 tggggggtaa tactttctct tgtacctatt gctgtagtaa cttttatgga gcatctgggt
     2881 gatattgcgg ccagcagtac ggtatgcggc aaagacttca tgaaagatcc gggagtgcac
     2941 agaacattga tgggagacgg agtgggaaca atgatagccg gagtcctcgg aggacctccg
     3001 aacactacat acggtgaaaa tacggcggta cttgctatta caaagaatta tgagcccaag
     3061 aatttgttct atgcaggtat aatggctgta atattcggtt cgataaccat attcggaacg
     3121 gctgtaggaa ctattccttc agcggtaata ggcggagcat ccatagtgtt gttcggaatg
     3181 atttccgcag caggcttgag agcattggta gacggcaaag tggacttttc cgataccaag
     3241 aatattctgg tagtttcggt aatactttca ataggtctcg gacttggcgc aatgagtctt
     3301 gcaggcgatg ttaccggtga tccgatatat aagataatga taggaacagt ggaaatttct
     3361 cctctggcaa tagcaacatt tgcaggtatt atacttaacc tggtaattcc ttccgacaac
     3421 agggaagaga agaaataagt gtaaattacg gctgccctca aaaaaggggg cagcctgctt
     3481 aaaaggaggg catgatgctt ttaaagaatg cttatatata caaagacgga agttttcatg
     3541 cctccgattt attcattatt gacgataagc ggtggatagc aggtaaaaat ctttccgctg
     3601 ttaaaacgga taaggttatt gatttgcaag gaaaatatat tattccgggg ttggtggatg
     3661 ttcatgtgca tctgagacag ccgggatttg agtttaaaga gacaatactg acaggatcaa
     3721 aaagcggggc ggcgggagga tttacgtcga tatgtgctat gcccaatgtg gtgccggcac
     3781 cggacagtat tgataatttc cgaagaattc ggaagattat agacagcgat gcatgtatca
     3841 atgtatatca gtacggttgt ataactaaaa aaagagaagg tatggaattg gcggatatcg
     3901 gtaagttgac cgggtatgta ataggttttt ccgatgacgg aaaccccgtt tcaagtgaat
     3961 cggttatggc ggcaatgctg gaacaggcat cattgcatga ctgcctgata gccgcccact
     4021 gcgaggactt gtcttatgaa gatcctgcaa agagagagta tatgcatata gaaagggata
     4081 tcgggttgtt gaggaagacg ggtatatcct gcagatatca tgtatgccat gtgtcaagaa
     4141 aagaaagtgt tgaacttatc cggcaggcaa aaaaagaagg aataaatatt acatgtgaga
     4201 cggctcctca ctaccttatc tttacaaaag aagatgttaa gtcggaaaac tatatcatga
     4261 atccgccttt gggaaataaa gaagacaggg aagctctgtt gcaggctgtc tgtgacggaa
     4321 cgatagatat tatcgccacc gaccatgctc ctcatacaaa ggaagacaaa gcagcgggtg
     4381 ccatgggagt tataggtatg gaaaccgctt ttagcagtat gtatacgtat ttggtggctg
     4441 caggtcgtgt ttcattggaa cgactggtgg agtttatgtc cgttaatccc gcccgtatat
     4501 tcgggatacc gcaatactcc gattttacgg tactggattt ggataaagaa tatatagtta
     4561 atgccaatga gtttttttca atgggaagaa atactccctt tgaaggcatg agaatgaagg
     4621 gcagagttat taaaacaata tgtaaaggga agatagtatg ggaatataca caatagaaag
     4681 cataaggcag cttacatatg atacttacga aatgacatta agcggtgata tgtcttggat
     4741 aaaagcaccc ggacaattcg tgaatataaa gattgacgga ttctttttaa gaagaccgct
     4801 gtcggtatgc gatattgatg acagatatat gaccctcata tttaaggttg taggacaagg
     4861 caccaaggca ctttccatga tgaaagtttc ttcgcagctg g
//
LOCUS       DAOPWC010000064         4893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_17366_length_4893_cov_3.867714, whole genome shotgun sequence.
ACCESSION   DAOPWC010000064 DAOPWC010000000
VERSION     DAOPWC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17366_length_4893_cov_3.867714"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /locus_tag="PKJ65_02255"
     CDS             <1..2084
                     /locus_tag="PKJ65_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40694.1"
                     /translation="ITPKNATKDHPAPGVVFTHGLYNNREMQLQNGIEAARRGYVVLL
                     IDRESHGHNDTTTYDNYGDSMLSAAKYLYNLTDPQGNRIVDGSRILVSGHSMGGSATN
                     GALMLDGVSNKTAITVGDTKYTWEGQVDEALSKGYHMGIISAGIVQANNAQNASYGTN
                     LKGVSVLKASSDEFFFASTTKNGIYRPLNLNQNTELLFTQGINEKVTVNRSIDGKLYV
                     KKGDDYVAITSSDTYHKKTQYYVFTKSGNSPFYLMSSQAMDFVGKPLESATGEQYEVI
                     NGGIYDFNKQGALIGQPEDKKYLSAQTGGAQQASDTVQIRAVYEAKENHPMNHFSRMS
                     AAGIIDFCYNVFGVAEGTTFKAPNNQTWWIKEVFAIFGIAGLFGLLVILVDLLLKTKA
                     FASLAAAEGDIPEAPVLLTKPRKHVFYWLSGILTAWYGYYAWENIVRGKNWYANSFWR
                     TLITDTTSEITGQTYTSWANAGTIAYWGICCATFALIITAVIWLINRVINMIVYKDDY
                     LAHEERPFLGFRIRSVGNIFKTLALAAILCFVFFGTIQLLWNVLVVDFRAWTFAFRIF
                     KTDRLLSYLRYVPFFFVFYMTNAALNINYRVKDLPEWVTVLINVVFNVGWLIISVWYF
                     NSYFINVGAFTNSTLSMQNIWTYPIIPCVATATIVSRRLYVRTGNAWLAGLVNATIMA
                     LVCCAGTSIGA"
     gene            complement(2164..3549)
                     /locus_tag="PKJ65_02260"
     CDS             complement(2164..3549)
                     /locus_tag="PKJ65_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNZ40695.1"
                     /translation="MSNSTIINKIVEKERIYFNEGHTLPISARIKSLKLLETSIRNNE
                     DMILSALKSDLNKSRYEGYMTELAIVFCELDHTLKNIKHWARPERKKVALSQAPAKAK
                     IIKEPYGTVLIMAPWNYPFQLCMVPLIGAIAAGNTAVVKPSAYAPAVSNAVCSVISEA
                     FPPEYVSVVEGGREENNALLEQHFDYIFFTGSPAVGKAVMQQAANNLTPVTLELGGKS
                     PVIAEQSADIKKTAKRILFGKLLNAGQTCVTPDYLLVQNSIKAELINEIKNHYNTMIP
                     DTKYKRENFPKIINKKHFDRIRQLMETQDILIGGNTYPDTLQIEFTLVNEPDTESPLM
                     QEEIFGPVLPVMGFDDINQAVNFVKSRPKPLALYLFTKDKSVEKTILRNLSFGGGCVN
                     DTVMHLSSPHLPFGGVGNSGMGSYHGKETFETFSHTKSILKKSWYLDIPVRYHPFQSP
                     DKKLPEWLFKK"
     gene            complement(3573..4397)
                     /locus_tag="PKJ65_02265"
     CDS             complement(3573..4397)
                     /locus_tag="PKJ65_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ40696.1"
                     /translation="MEVGLVTRSLANHTPEETVDLLKKFGFTSTEISFAFSNLYVYQY
                     NGYKPMDELTDEAAKDIIKLFRDNSIDVVAIGAFSSLFEPDKEKQENNFKAYERYIQV
                     AANNGIEYVATESGFVPGKRGIDFFTFEADFAYFKSNMIRLLDIAKHYGVSIAYEPCI
                     FDFAPSAKRTRDLIWQCERDNFKILLDPANLIANSDEEDIFKYLKDYIAYFHGKDRKV
                     NDAKGRNVGEGDIDWVKFLSLRKKYTPDAAFILEYCNKTNCEMVKERVLEYAKQVV"
     gene            complement(4413..>4893)
                     /locus_tag="PKJ65_02270"
     CDS             complement(4413..>4893)
                     /locus_tag="PKJ65_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HNZ40697.1"
                     /translation="VLAKLLAVNLQVIRIGTEFVSIAMASILFGPAYGALSAVLADLL
                     GSVLFPTGPYSPVITIVAIANGLIYGLFLHNKEITVKTTIFAVLTKALISDFFLMSIA
                     LYMLYRIPINELLVARSIKTAAVIVIETLVIYLVIRPVLNIFYKKGWIVPEDIIKKD"
BASE COUNT         1367 a         1077 c         1013 g         1436 t
ORIGIN      
        1 tgatcacacc taaaaatgcc accaaagatc atccggctcc cggtgtggtc tttacacacg
       61 gtctgtacaa caaccgtgaa atgcagttgc aaaacggcat cgaggctgcc cgcagaggtt
      121 atgtcgtatt gctgattgac cgtgaatctc acggacacaa cgacacaacc acttatgaca
      181 attacggtga ttcgatgttg tccgcagcca agtaccttta caatctgacc gatccgcaag
      241 gtaacaggat tgttgacggc agcaggattt tggtttccgg tcactccatg ggcggcagcg
      301 ccacaaacgg tgccttaatg cttgacggtg tatccaataa gaccgctatc accgtaggtg
      361 acaccaagta cacatgggaa ggccaggttg atgaggcatt gagcaaagga taccacatgg
      421 gtataatttc cgcaggtatc gtccaggcca acaatgctca gaacgccagt tacggtacca
      481 accttaaagg tgtttcggtt ctcaaggcat ccagcgacga gttcttcttt gccagcacaa
      541 ccaagaacgg catctatcgc ccgctcaacc tcaaccaaaa cacggaattg ttgttcacac
      601 aaggtataaa tgaaaaggtt accgtaaaca gaagtataga cggtaaattg tatgtaaaga
      661 agggggatga ttatgtagca ataacctctt ccgataccta ccacaagaaa acacaatact
      721 atgtgttcac aaagagcggc aattccccgt tctatctcat gtcctcgcag gctatggact
      781 ttgttggcaa acctttggaa tcggcaacag gtgagcaata cgaagttatt aacggcggaa
      841 tctatgattt caacaaacaa ggtgctctga taggtcagcc tgaagacaaa aaatacctgt
      901 cggcacagac cggcggagcg caacaggcaa gtgatacggt tcagattcgt gcggtttacg
      961 aagccaaaga aaaccacccg atgaatcact tctccagaat gagtgcagcg ggaataatag
     1021 acttctgcta caatgtattc ggagttgccg aaggcacaac cttcaaggca cctaataacc
     1081 agacatggtg gataaaggaa gtattcgcca ttttcggaat tgccggtctg ttcggactgc
     1141 ttgtcatact ggtagattta ctgcttaaga caaaagcatt cgcttcactg gcagctgccg
     1201 aaggcgatat tcccgaggct ccggttcttc ttacgaaacc ccgcaaacat gtattctact
     1261 ggctgagcgg tattctgact gcatggtacg gatattatgc ctgggagaat atagtgaggg
     1321 gtaaaaactg gtatgcaaac agtttctgga gaacactgat taccgacacc acaagcgaaa
     1381 ttaccggtca gacctacacc agctgggcaa atgccggtac cattgcttac tggggtatat
     1441 gctgtgcaac atttgcactg ataataacgg cggtcatctg gctcataaac agagtcatca
     1501 acatgattgt ttacaaagat gattatcttg cacatgaaga gagaccgttc ctgggcttca
     1561 gaattcgcag tgtaggtaat atcttcaaga cgcttgcact tgcagcaata ctttgcttcg
     1621 tgttcttcgg aaccatacaa ctactttgga atgtgttggt tgtggacttc agagcatgga
     1681 catttgcatt cagaatattt aagaccgaca gactgctgtc ctacttaaga tatgtgccgt
     1741 tcttctttgt attctacatg accaatgcgg cattgaatat aaattacagg gtgaaggatc
     1801 tgcccgagtg ggtaaccgtt ctcataaatg tagtattcaa tgtaggctgg ttgattataa
     1861 gtgtgtggta cttcaacagc tacttcatca atgtaggagc attcacaaac agcacgttaa
     1921 gcatgcagaa tatatggaca tatcccatca ttccatgcgt tgcaaccgca actattgttt
     1981 ccagacgtct gtatgtacgt acaggaaatg catggcttgc cggtcttgta aatgccacta
     2041 taatggcact ggtctgctgt gcaggcacat ccataggtgc ataagagaaa cggtaattca
     2101 caaaagataa atcaaacgga acgagaaatc gttccgtttt tcttcttacc cggttaattc
     2161 tgtttacttc ttaaacaacc attccggcag cttcttatcc ggagattgga aaggatggta
     2221 gcgtacggga atatccaaat accatgattt tttaagtata ctctttgtgt gcgagaaggt
     2281 ctcaaaggtc tctttaccgt gatagctgcc cattccgctg ttacccaccc caccgaacgg
     2341 cagatgcgga ctgctaagat gcataaccgt gtcattgaca caaccgccgc cgaaagaaag
     2401 atttctcaga attgttttct ctaccgattt atcttttgta aaaaggtaca gagccaaagg
     2461 cttgggacgg ctcttaacaa agtttacagc ttggtttata tcgtcaaaac ccattacagg
     2521 aagaacagga ccgaaaattt cctcctgcat aagaggactt tcggtatccg gttcgtttac
     2581 taaagtgaac tctatttgca gagtatcggg atatgtattt cccccaatca aaatgtcttg
     2641 ggtttccata agttgcctga tacgatcaaa atgctttttg tttataatct tggggaaatt
     2701 ttctcttttg tattttgtgt cgggaatcat tgtattgtaa tgattcttta tttcattgat
     2761 aagttccgcc tttatgctgt tttgcactaa aagatagtcg ggagttacac aggtttgtcc
     2821 tgcgttcaga agttttccga agagtatccg cttggcagtt tttttaatat ccgcgctttg
     2881 ttccgctata acggggctct ttccgcccag ttccagagta acgggtgtaa gattatttgc
     2941 agcctgttgc attacggctt ttcctacagc gggtgatccg gtaaagaaga tatagtcaaa
     3001 atgctgctcc aataaagcat tattctcctc tctgcctcct tccacaacgg atacatattc
     3061 cggaggaaat gcttcgctta taacggagca aactgcattt gatacagcag gagcatatgc
     3121 cgaaggcttt acgacagccg tattacctgc ggctatggcg cctataagcg gaaccatgca
     3181 aagttgaaaa ggataattcc aaggggccat tatgagaaca gtaccgtaag gctcctttat
     3241 tatcttcgct ttagccggag cctgtgaaag agcaactttc tttctttcag gtcttgccca
     3301 atgttttata ttttttaaag tatggtccaa ctcgcaaaat acaattgcaa gttcggtcat
     3361 atatccttca taacggctct tgttcaaatc gcttttcaga gcggaaagaa tcatatcttc
     3421 attatttctt atggatgttt ccagcagttt aagagattta atccttgcac ttatcggcaa
     3481 agtgtgtcct tcattgaaat atatgcgctc tttttctaca atcttattta taattgtgct
     3541 gttgctcatg tcggaaactc ctttatcggt ttctatacca cctgttttgc gtactccaac
     3601 actctttcct ttaccatctc gcagttggtt ttattacagt attccaatat gaaagcggca
     3661 tcgggggtat actttttacg aagcgacaga aacttcaccc agtctatatc gccttctccc
     3721 acattcctgc cctttgcatc atttactttt ctgtctttac cgtggaaata cgcaatatag
     3781 tctttaagat atttgaatat atcttcttca tcggaattgg caatgagatt tgcgggatct
     3841 aacaatattt tgaagttatc tctttcacac tgccagatta agtctctggt tctttttgcg
     3901 gaaggtgcaa aatcaaatat acagggttca tatgcaatgg atactccgta atgctttgcg
     3961 atatccaaca gtcttatcat gttggatttg aaataggcaa aatccgcctc aaaggtaaag
     4021 aagtctatgc ctctttttcc cggaacaaat cccgattcgg ttgccacata ctcaataccg
     4081 ttgtttgcgg ctacctgaat gtatctttca tatgctttaa aattgttttc ctgtttctcc
     4141 ttatccggct cgaacagtga ggaaaatgca cctatggcaa ctacatctat cgaattatcc
     4201 ctgaacaatt tgattatgtc tttagcggct tcatcggtca actcgtccat cggtttgtaa
     4261 ccgttgtact gatatacata taaattactg aatgcaaaac ttatctctgt agaggtaaaa
     4321 ccgaatttct ttaataagtc taccgtttcc tccggtgtat gatttgcaag actcctcgta
     4381 accaatccta cttccattgt gtaattcctc ctctaatcct ttttaattat atcttccggt
     4441 acaatccatc cttttttata gaagatattg agaaccggtc tgataactaa ataaataacc
     4501 agcgtttcta tcactattac cgctgcggtc ttaatgcttc ttgctaccaa taattcatta
     4561 atcggtattc tgtacagcat atacaatgct atgctcataa gaaaaaagtc gcttatcagg
     4621 gctttggtca gtacggcaaa aattgtggtt tttacggtta tttctttatt gtgaagaaat
     4681 aagccgtata taaggccgtt agcaatggcc acaatggtta ttaccggaga atacggtccg
     4741 gtagggaata aaacgcttcc cagcaaatcg gcaagcacgg cggataatgc tccgtaggcg
     4801 ggaccgaata atattgatgc catagcaatg gaaacaaatt ccgtccctat ccttataacc
     4861 tgaagattaa cggctaagag ttttgctaaa aca
//
LOCUS       DAOPWC010000065         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_17911_length_4780_cov_3.412275, whole genome shotgun sequence.
ACCESSION   DAOPWC010000065 DAOPWC010000000
VERSION     DAOPWC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17911_length_4780_cov_3.412275"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKJ65_02275"
     CDS             complement(<1..734)
                     /locus_tag="PKJ65_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNZ40698.1"
                     /translation="MERNISAIISEGKTFIGIEFGSTRIKSVLIDDAFNVLASGNFDW
                     ENKLEGGYWTYGKDEIIEGMQISYRNLAADVYEKYGIKLIKTAAIGISGMMHGYLAFD
                     SEDNLLVPFRTWRNITTGVAAKKLSETLSFNIPKRWSISHLYQAILDKEPHVDKIDHI
                     NTLAGHIHYLLTGKKVVGIGEASGIFPLSGNGYNKEMLKKTEELFLASGYSLKLEKLL
                     PSVKKTGETAGYLTERGAKLLDPTGTL"
     gene            complement(1463..2461)
                     /locus_tag="PKJ65_02280"
     CDS             complement(1463..2461)
                     /locus_tag="PKJ65_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40699.1"
                     /translation="MKPFRIAQIGTSRWSHGNECFRALKNNPDVFEIAGYCFPENEPE
                     KFPKQMNIFEGYRELTLDEILNDSTIGAVAIETEEKYLTKYALSAVRHGKHIHMEKPG
                     GLDSAEFEQVITIAKTNQTVFHTGYMYRYNPYVQELLEQIKCGELGEIISVEAQMNCY
                     EPLECRKWLEDYPGGMMFFLGCHLIDLIYTIQGRPKAIIPLNKSTGLDGVSCKDFGMV
                     IFEYDKGVSFAKTSAVEGGGFERRQLVVCGSKKTVELHPLERHIENRLLTTGRYTREN
                     STLWNTVHEQEFAKPFDRYSPMMLNFAQMVRGEKKNPYDYDYELTLHQLVIKASGG"
     gene            complement(2458..3477)
                     /locus_tag="PKJ65_02285"
     CDS             complement(2458..3477)
                     /locus_tag="PKJ65_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNZ40700.1"
                     /translation="MTRKSILFVQVGKAELVDIPLPELKTNEVLVRTAISTISSGTER
                     ANLCGDLNVSPMQPTLTVAKFPRSSGYSTSGTVEAVGAAVKKVAVGDRVAMCWSCHTD
                     LNVLSENLVYKLDDNISFETAALLHIGTFPMAAIRKTRLEFGEPAIVMGQGILGQIAV
                     LQLKVAGASPVIAVDPVKERRDIALNLGADFALDPTDSEFVNNVKAICPLGVPVAIEV
                     TGVGKGFDQVLDCMAQFGRVALLGCTRNSDFTIDYYRKIHRPGITVVGAHTMARPEVN
                     SYPGYWTRTDDMEAQLRLIKYGRLSYDGLVKDTRSPVEAPEVFNRLANDPHFPITQFD
                     WRRLL"
     gene            3599..>4780
                     /locus_tag="PKJ65_02290"
     CDS             3599..>4780
                     /locus_tag="PKJ65_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40701.1"
                     /translation="MKSIVKRSYVPKSVIYAKSYVPKNVILLIFNTFADIMYVRKGGV
                     MKRKIYKDLLNWKNSGDRKPLILQGSRQVGKTYIVNLFGADNYANVVYCNFEKEPDLA
                     DFFTDLKPVNILEKLGNYKRKEILPSHTLIIFDEIQACPEALTSLKYFCEEANEYHLI
                     ATGSLLGVSVNRKESSFPVGKVEFLNMYPMDFEEFLMANDVSYLIDEIKDCYNLNKSL
                     PEPFHREALRLYKIYLYTGGMPEVVEEYLKNNNYELVKIKQLAILEAYFNDMGKYNRA
                     TEIPKTKQVYKNISTQLARENRKFKYSSIKSGGRASEFANAIEWLCLAGVANQLYRVE
                     HIKLPLNAYKSLNDFKFYMNDVGLCSASQDVFINDILYDNPAFNDFKSGLTENYVNNQ
                     LI"
BASE COUNT         1396 a          974 c          947 g         1463 t
ORIGIN      
        1 agtgttcctg tgggatccaa taatttggca cctctttcgg taagataacc tgccgtttcc
       61 ccggttttct taacggaagg cagcagtttt tccaatttca gagagtaacc gcttgccaaa
      121 aacagctctt cggtcttttt cagcatttct ttgttgtacc cattacccga taaaggaaat
      181 atgccgctcg cttcaccgat tcccactact ttttttcctg tgagaagata gtgaatatgt
      241 cctgcaagag tgtttatatg gtcaatttta tctacatgcg gttccttatc caagattgcc
      301 tgatacaaat gggaaatgct ccacctcttt gggatattga atgataaagt ttcactcaat
      361 ttctttgcag ctactcccgt agttatattt cgccaggtgc ggaagggaac taacagatta
      421 tcttcactgt caaatgccaa ataaccgtgc atcattccgg atatgcctat ggctgcggtc
      481 tttatcaatt ttatgccgta cttttcataa acatcggctg cgagattccg ataggagatt
      541 tgcatcccct caattatttc gtccttcccg tatgtccagt aaccgccttc aagtttgttt
      601 tcccaatcga agtttcccga agcaagaaca ttgaatgcat catcaattaa aacagatttg
      661 attcttgtag agccgaactc tatgccgata aaggttttgc cttccgatat tattgcagat
      721 atgtttcttt ccatattaaa tactcccgtc agatgtatta tactatagaa attatgtgaa
      781 ttatcaattt aagacactgt tttctgaaaa aagaataggg tgatattaag caggtatggt
      841 atcggataaa taccggaaga ataatcagaa tcttataaaa tgtgattttg cctaattatc
      901 tttgacgaag aataaaaaac gcctgcggaa tgcgggtgtt cagtaagtgt gaatgtatta
      961 ttaattatga tttgattatt ccggctgcta ataaaataac ataaaaagga aagaacaata
     1021 ttataagtat caattttata atttcaacag ttctgcgcat tattctttat cctccaaaag
     1081 cctttatcac aagctgatgc ggtttcagtt cgtaatcgta atcataagga ttcttcttct
     1141 caccatatac catatgcggg tgttcagtaa gtgtgaatgt attattaatt atgatttgat
     1201 tactccggct gcttttaaca gaacaacaaa cggccaaaat attatcatca gtattgcttt
     1261 tattatgtaa aaggttcttg tcattaagct tattatatcc tccgcatatt tattttatca
     1321 tctggtgagg ctgctgttca agggtttttt ccatattaag tacattttct gtaaaaaaag
     1381 cgtctatata tttcaagaac attcagttct tcttccttga ccgtaaagcc cgagtgtgcc
     1441 gctgcctgtt cagaagcatt ttttatcctc cggaagcctt tattacaagc tgatgcagtg
     1501 taagttcgta atcgtaatca tagggattct tcttctcgcc ccgtaccatt tgtgcaaaat
     1561 tcaacatcat aggggagtat cggtcaaacg gtttggcaaa ctcctgctca tggactgtat
     1621 tccaaagagt ggaattttcc cgtgtgtaac gaccggttgt cagcagtctg ttttctatgt
     1681 gtcgctccaa aggatgcagt tccacggttt ttttgctacc gcaaacaacc aactgacgcc
     1741 gctcaaaccc gccgccttct accgctgatg ttttggcaaa agacacgcct ttgtcatatt
     1801 cgaatattac cataccgaaa tccttgcagc taacaccgtc cagacctgtg gatttgttca
     1861 gcggaatgat tgccttcggt cgaccttgaa tggtgtaaat taaatcaatc aggtgacacc
     1921 ccagaaagaa catcataccg cccggataat cttccagcca tttccgacac tccaacggtt
     1981 catagcagtt catctgcgct tcaacactga taatttcgcc gagttcaccg catttaatct
     2041 gttccaacag ttcctgtaca taaggattat atctatacat atagccggta tgaaacaccg
     2101 tttgattggt ttttgcgatt gttataacct gttcaaattc cgcagaatcc aaaccgccgg
     2161 gcttttccat gtggatatgc ttgccgtgcc ggacagctga aagtgcatat ttagttaagt
     2221 atttttcctc ggtttcgatt gcaacagcgc cgatagtgga atcatttaat atttcgtcaa
     2281 gcgtcaattc gcggtaaccc tcgaatatat tcatttgctt tggaaacttt tccggctcgt
     2341 tttcggggaa gcagtatcct gcaatctcga acacgtccgg attattcttc aaggcacgaa
     2401 aacattcgtt accgtggctc caacgactgg tgcctatttg ggcgattctg aacggcttca
     2461 taaaagtctt ctccaatcaa attgagtaat aggaaagtgc ggatcgttag ccaaccggtt
     2521 aaaaacctcc ggtgcttcaa cgggacttct ggtgtccttg accaaaccgt cataggataa
     2581 ccgaccgtat ttgattaaac gaagttgcgc ttccatatca tcggtccgtg tccaatagcc
     2641 cggataagag tttacttcag gacgcgccat ggtgtgagca ccgacaaccg tgatgcccgg
     2701 ccgatggatt tttcggtagt agtcgatggt gaaatcactg ttacgggtgc aaccgagcaa
     2761 agcgacacga ccgaactgtg ccatgcaatc cagcacctga tcaaatccct tgccgacgcc
     2821 ggtgacttca atagctacgg gaacacctaa aggacaaatt gccttgacat tgtttacgaa
     2881 ttcagaatcg gtcggatcca gcgcaaaatc agctcccaga ttcaaggcaa tatcacggcg
     2941 ttctttaacc ggatcaaccg cgatgaccgg tgaagcaccg gcgactttca gttgtaatac
     3001 ggcaatctga ccgagtatgc cttgtcccat gacgatagcc ggttcaccaa actccaaccg
     3061 tgttttgcgg attgccgcca tagggaaagt tccgatgtgg agcaaagccg ctgtttcaaa
     3121 tgaaatattg tcgtccaatt tatataccag gttttcggaa agaacattca aatcggtgtg
     3181 acatgaccag cacatggcaa cgcggtcacc gacggcaact tttttcaccg ccgcacctac
     3241 ggcctctacc gtacccgatg tgctgtaacc ggacgagcgc ggaaatttgg caaccgtaag
     3301 ggtcggttgc atcggcgaaa catttaaatc cccgcacaga ttagcgcgtt ccgtaccgct
     3361 gcttatagta gaaatagcgg tacgcaccag tacctcgttt gtttttaatt ccggtaaagg
     3421 tatgtcaacc agctccgctt ttccgacttg gacaaacaaa atggattttc tcgtcataaa
     3481 atatcctccc gaatttcgat accataatta tattggataa acaaagcggt tacaacgggc
     3541 tatgtaattc agatgttttt tataacctga aagctgtgag tggaaacact tatttctctt
     3601 gaaaagcata gtaaaacgca gttatgtgcc aaaaagtgtg atttatgcaa aatcttatgt
     3661 gccgaaaaat gtgattttat tgatttttaa cacatttgct gatattatgt atgttagaaa
     3721 gggaggagtt atgaaacgga aaatttataa agatttattg aactggaaaa actccggtga
     3781 caggaagcct ttgatattac aaggatccag gcaagttggc aaaacatata ttgttaattt
     3841 gtttggagca gataattatg ccaatgtggt ttattgtaat tttgagaaag agcccgattt
     3901 agcggatttt tttacagatt taaagcctgt aaatatttta gagaaattag gcaattacaa
     3961 aagaaaagag attttacctt ctcatacttt gattattttt gacgaaatac aagcctgccc
     4021 tgaagcttta acctctctta aatacttttg cgaagaagca aatgaatatc atttgattgc
     4081 aaccggttcg ttgctcggtg tttctgttaa cagaaaggaa tcatcctttc ctgtcgggaa
     4141 ggttgaattc ctaaatatgt atcctatgga ttttgaagag tttttgatgg ccaatgatgt
     4201 tagttattta atagatgaaa ttaaggattg ttataacttg aataagtcgt tacctgaacc
     4261 ttttcaccgg gaagctttga gattatataa aatatatttg tataccggcg gaatgcctga
     4321 agttgttgaa gagtatttga aaaataataa ctatgaactt gttaaaatta aacaactggc
     4381 aatattggaa gcctacttca atgatatggg taagtataac agagcaactg aaattccgaa
     4441 aacaaaacaa gtatataaaa atataagcac tcaacttgca agagaaaaca ggaaatttaa
     4501 gtattcttcc ataaaaagcg gtggcagagc aagcgaattt gcaaatgcta ttgaatggtt
     4561 atgtttagca ggtgttgcaa atcaactata tagagtggaa catattaaac tcccgctaaa
     4621 cgcttacaaa tcactaaatg atttcaagtt ttatatgaat gatgttggat tatgctctgc
     4681 cagtcaggat gtttttataa atgacatctt gtatgataat ccggcattca atgactttaa
     4741 aagcgggtta acggaaaatt atgttaacaa tcagttaata
//
LOCUS       DAOPWC010000066         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_18295_length_4710_cov_3.073255, whole genome shotgun sequence.
ACCESSION   DAOPWC010000066 DAOPWC010000000
VERSION     DAOPWC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18295_length_4710_cov_3.073255"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKJ65_02295"
     CDS             <1..253
                     /locus_tag="PKJ65_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ40702.1"
                     /translation="SFIGNITSFNIIYLLTGGMPKVTGGYIAGGTDLLVTWLYKLTID
                     EQQYNLGAVIGILTFLLTSTVTLITYRRSKSYKEEDAFQ"
     gene            250..1167
                     /locus_tag="PKJ65_02300"
     CDS             250..1167
                     /locus_tag="PKJ65_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ40703.1"
                     /translation="MSEKNENVKVKKIRPNYLNLSSSEKISRLGAYILLIALCLIWLY
                     PIVWIVLNAFRTEFNPDGTMVGTVVSHYWPKQVGWDNFKILFTNTYFPVWFRNTLVVA
                     VFTCILSTMLNMSVSYVMSKVKFKMRKPFMNIAMVMGLFPGFMSMIAIYYILKALGLT
                     QTLFALILCYSAGAGLGFYVGKGFFDTVPNALIEAARIDGAGEARIFATIILPISKPI
                     IVYTALMSFTGPWMDFIFARVILGEQNTHLHTVAVGLYYMMYGQIVDTSRFTVFAAGC
                     VCVAIPIVALFMYLQKYYIEGSTAGSVKG"
     gene            1164..2783
                     /locus_tag="PKJ65_02305"
     CDS             1164..2783
                     /locus_tag="PKJ65_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ40704.1"
                     /translation="MMKNITKKILCILLSILILLTLVSCSTDAFRGEKAIKIEQMPAD
                     NIPGVYYEIFVGGFSDSNKDGTGDIQGIINRIDYLNDGNPLSGKSLGIDGIWLMPIMQ
                     AGSYHKYDTVDYYSIDYNYGTMEDFENLIAVCNEKGIDVIIDLVLNHTSEYNKWFTQL
                     KKAIKEGDKDNKYLDWYSITTDSSKGWHKLATDPDGVDYFYEGNFSPSMPELNMDNKE
                     VLDEIENIVKFWLGKGVKGFRLDAVKYVYLNDDTRNIEFWKWFADTCKKYDEDVFLVA
                     ENWSGNTSIIDYYAALDCFDFGMSGSAGQVALTVNGIFNLNDFVKNICRYKENVEKSN
                     PDAVLKPFISNHDMDRCAGYLPVKEYRMHMAANMYILTSGNPFMYYGEEIGMYGSRAT
                     SSTDANRRLAMLWGDNDTVKDPPGTTFPAERQVNGTVKKQVKRADSLYTHYKKLLALR
                     KANPEIAVGHYTPLYFEGYNLFGGFLVEHNNVVKGVFHNLSDKPITIDLSPYLNAGTS
                     FNSISGYIGQGKASIKKNTLTLDGMTSVILK"
     gene            2929..3513
                     /locus_tag="PKJ65_02310"
     CDS             2929..3513
                     /locus_tag="PKJ65_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40705.1"
                     /translation="MKKIKVIAIVLTLVLALGSLVACTPDTVLENTEKDYYVTGQFAG
                     WGDAVGKDQFRMTAVSLKDARVAALKAQLKGAKYLYVLEHVVITDSGAGWTAQYVENG
                     AVKDCDGNQTMKWLQVAKGQEAPDWWAQSPESGPVTSLTPDLLWIPGFTETPAVGPDW
                     NGNPVVLKAGTYTVVFAMVEKDTGLEKVAGLIAE"
     gene            complement(3580..4506)
                     /locus_tag="PKJ65_02315"
     CDS             complement(3580..4506)
                     /locus_tag="PKJ65_02315"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNZ40706.1"
                     /translation="MRLTNFIENGKFYKANYHAHSTRSDGQLPQEEAVNVFKEHGYNF
                     LCLSEHNIVTKTDKYNTDDFILVPGVELHSNRNCPLTIHHLLGLTTMDNDKIYDGMRL
                     EDIPFTESKESLLKLEKTLLDLDLLTVYCHPTWSRVEPHQYDFLKDTHIVEICNWGTV
                     YSQNHDNDRVYWEMLLKQGKQIWGMANDDSHRREQYCGGWNMVKCEEFTVESMLDSLR
                     KGSFYATTGPEIYDFYIEDGYGFVKCSEAKTITFITLSRWGKCYRDPAAKLTEASLNL
                     SSSAYMVRVEVEDKYGNVAWSNPIMRMDRFEY"
BASE COUNT         1409 a          950 c          987 g         1364 t
ORIGIN      
        1 ctcattcata ggcaacatta caagtttcaa tatcatatat ctcctgaccg gcggtatgcc
       61 caaagttacc ggcggatata ttgcgggagg taccgatctt cttgttacct ggctttacaa
      121 acttaccatt gatgaacaac agtacaattt aggagctgtg ataggtattc ttaccttcct
      181 cttaacatcg acggttactc tgataaccta ccgcagatcc aaatcctata aagaggagga
      241 cgcattccaa tgagtgaaaa aaatgaaaat gtaaaggtaa agaaaataag accgaattat
      301 ttgaatcttt ccagcagtga gaaaatttcc agattgggtg catatattct cctcatcgct
      361 ttgtgcttaa tatggcttta tcccatagtg tggatagtgc tcaatgcatt ccgtacggag
      421 tttaatcccg acggaacgat ggtaggtact gttgtatccc actactggcc caaacaggtg
      481 gggtgggata atttcaagat actttttaca aacacatatt ttcccgtatg gttcagaaat
      541 actttggttg tggctgtatt cacctgtatt ctgtccacaa tgctcaatat gtcggtatct
      601 tatgtcatga gtaaggtaaa gttcaaaatg agaaaaccgt ttatgaacat agccatggta
      661 atgggtttgt tccccggatt tatgtccatg atagctattt actacatttt gaaagctctg
      721 ggactgacac agacactgtt tgctttgata ctctgctact cggcaggtgc gggattggga
      781 ttttatgtag gcaaaggctt tttcgatacc gtacccaatg cgcttattga agcggcacgc
      841 atagacggcg caggagaagc acgaatattc gctaccatca ttctgcccat aagtaaaccc
      901 ataattgtat ataccgcttt aatgagtttt accggaccgt ggatggattt catatttgcc
      961 cgtgttatac tgggtgagca gaacactcat ctgcataccg ttgcggtcgg gttgtattac
     1021 atgatgtacg gacagattgt cgacacatcc cggtttacgg tttttgcagc aggctgtgta
     1081 tgtgtagcca tcccgatagt tgcgttattc atgtacctgc agaaatacta catagaagga
     1141 agcacggcgg gttcggtaaa aggatgatga aaaacataac aaaaaaaata ttatgcatac
     1201 ttttaagcat cttaatactg cttacccttg tttcctgcag tactgatgcg tttagaggcg
     1261 aaaaagccat aaaaatagaa caaatgccgg cggataatat ccccggcgtt tattatgaaa
     1321 tattcgtggg aggtttctcc gacagcaata aagacggaac gggcgatata cagggaataa
     1381 tcaaccgtat cgattacctc aatgacggta accctttaag cggtaaaagt ctcggaatag
     1441 acggcatctg gcttatgccg ataatgcagg caggttccta tcataaatat gacaccgtag
     1501 attattacag tattgattac aactacggta ctatggaaga ttttgagaac ctcatagccg
     1561 tttgcaatga aaaaggtatt gatgtgataa ttgatctggt tctcaatcac acatcggaat
     1621 acaacaaatg gtttacccaa ctgaaaaagg caattaaaga gggagataaa gataataaat
     1681 atttagactg gtacagtatt acaaccgatt cgtccaaggg atggcataag cttgcaacag
     1741 accccgacgg tgtcgactat ttttacgaag gcaacttctc cccttccatg ccggagctga
     1801 atatggataa caaggaagtg cttgacgaaa ttgaaaatat cgtcaagttc tggctgggaa
     1861 aaggcgtaaa gggtttccgg ctggatgccg ttaaatatgt ttatctgaac gatgataccc
     1921 gtaacataga gttctggaaa tggtttgccg atacctgcaa aaagtatgac gaggatgtat
     1981 tcttagttgc ggaaaactgg tcaggtaata ccagcataat cgattattat gcggcattgg
     2041 actgctttga tttcggtatg tcgggctcgg ccggacaggt ggcacttacg gtaaacggca
     2101 tattcaatct gaacgacttt gtcaaaaaca tatgccgcta caaagaaaat gtggaaaaat
     2161 ccaaccccga tgctgtactc aaacctttca tttccaatca cgatatggac agatgtgcag
     2221 gatacttacc ggtcaaagag tacagaatgc atatggccgc aaatatgtat atacttactt
     2281 cgggtaatcc ttttatgtat tacggagaag aaataggtat gtacggaagc agagccacct
     2341 cttcaaccga tgccaacagg cgtctggcaa tgctgtgggg agataacgat accgtaaaag
     2401 atcctccggg gacaacattc cctgctgaaa gacaggtaaa cggtaccgtt aagaaacagg
     2461 taaagcgggc cgacagttta tacactcatt acaagaaact gttagcatta agaaaggcaa
     2521 atcccgaaat tgcggtggga cattatactc ctctgtattt tgaaggttat aaccttttcg
     2581 gaggatttct tgttgagcac aataatgtag ttaaaggagt attccataac ctttccgata
     2641 aacctataac cattgatttg tcaccgtatt tgaatgccgg tacttcattc aactctataa
     2701 gcgggtatat cggacaagga aaagcaagca taaaaaagaa tactctgaca ctggacggaa
     2761 tgacttcggt tatactgaaa tgataaaagt ttacaaaata ttaacactga gggaaaagtt
     2821 cacaaatttt tcacaatttg cctaaaatcc cttgactgta ttaatcgttg tgatagaata
     2881 ggattgttca ttataagtag tactagatac taaaaacagg aggaatttat gaaaaaaatc
     2941 aaagttatcg ctattgttct gacactggtt ttggcactcg gatcacttgt cgcttgcaca
     3001 cctgataccg ttctggaaaa cacagagaaa gactattatg taacaggaca gttcgccgga
     3061 tggggcgatg cagttggaaa agatcagttc agaatgaccg ctgtttcctt aaaggatgca
     3121 cgtgttgctg ctctgaaagc tcaacttaaa ggtgcaaaat atctctatgt ccttgagcac
     3181 gtagtaatca ccgactccgg tgcaggctgg acagctcaat atgtagagaa cggtgctgtt
     3241 aaggattgcg acggaaacca gacaatgaag tggttacagg ttgcaaaagg acaggaagct
     3301 cccgactggt gggcacagag tcccgaaagc ggcccggtta cttccctgac acccgatctt
     3361 ctctggattc ccggatttac cgaaaccccg gcagtaggac cggactggaa cggaaacccc
     3421 gtagtactca aagcaggcac atacactgtt gtatttgcta tggttgagaa agataccgga
     3481 ttggaaaaag tagcaggcct tatagccgaa taagcaaagt aaactacaca aaaaggcggc
     3541 tttcatagcc gctttttttg tgtctttatt tactaactac taatactcaa atctgtccat
     3601 tctcatgatg ggattggacc aggctacatt gccgtattta tcctctactt ccactctgac
     3661 catatatgcg ctgctggaca gattcaatga agcttcggta agttttgcag ccggatcgcg
     3721 atagcatttt ccccatctgc tcaatgtaat gaacgttatg gtcttagctt ccgaacattt
     3781 aacaaatccg tagccgtcct ctatgtagaa atcatatatt tccgggccgg tagttgcata
     3841 aaaactgccc tttcttaaac tgtccagcat agactcaacg gtaaattcct cacacttgac
     3901 catattccat cctccgcaat actgttctct tctgtgcgaa tcgtcgttgg ccattcccca
     3961 tatctgtttt ccctgtttta acagcatttc ccagtacaca cggtcattat catgattctg
     4021 cgaatatacc gttccccagt tgcatatttc cacaatatgt gtgtctttta agaaatcata
     4081 ctgatgaggt tccacacggc tccatgtggg atgacaataa actgtcagaa ggtccaaatc
     4141 cagcaatgtc ttttccagtt tcagcaacga ttccttactc tcggtaaaag gtatatcctc
     4201 cagtctcata ccgtcataaa tcttatcatt atccattgtt gtaagtccca acaggtgatg
     4261 gatagtaagc gggcaattgc gattggaatg taattccacc ccgggaacaa gtataaaatc
     4321 atcggtgtta tacttatcgg tctttgtcac tatattatgc tcggataaac aaaggaaatt
     4381 atagccgtgc tctttaaaaa cattgacagc ttcttcctga ggcagctgtc cgtcacttct
     4441 tgtcgaatgt gcgtggtaat tggccttata gaatttgccg ttttcaataa aattggtcag
     4501 tctcatatat tcatcctcat ttcacaatac atacatatgt atgcgtttgt cagttataca
     4561 agattatata tcatgtatat ggtttttaga ttatttttta tttatattga tgtaaattta
     4621 ttattcacgc atatacgcct taatgaacgg ttattatggt ataattttta tatcattata
     4681 atttagattt attgatttta aattttgtaa
//
LOCUS       DAOPWC010000067         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_18358_length_4694_cov_3.381332, whole genome shotgun sequence.
ACCESSION   DAOPWC010000067 DAOPWC010000000
VERSION     DAOPWC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18358_length_4694_cov_3.381332"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..2603)
                     /locus_tag="PKJ65_02320"
     CDS             complement(141..2603)
                     /locus_tag="PKJ65_02320"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNZ40707.1"
                     /translation="MKKTGFYRSIVSIALSVLLICAFLPVFAYDVNGYHISDYDIAID
                     VGEDNVMYITENIAAVFTESGKHGIIRTIPTVFGNQRVFIDDIYVDKTYVVSTEADYV
                     SIRIGDADKTVKGEVKYKITYRYDYGDDQQDAYDKIYHNILGTDWDCDIYKVTFTVNM
                     PKSFDKEKISITYGDRYSDSKYTGYTVSGNSIKGQLTDLSAYQGITLALELPEGYFVN
                     ERPVTVYDRHGYHISHYETVIDVGENNVMHITENITADFTAYGKHGIIRTLPTVFEGK
                     RVYVDNIYVDKTYVVEHAREYVSIRIGDADKIVEGEVKYKITYDYDYGDDLEEAYDKI
                     YHNILGRDWDCDVYLVTFTVNMPKPFDKEKISITYGDWYSDTRYTGYTVTGNTVKGQL
                     SGLSSYQSVTLALELPEGYFVNERPAAGVRIPVIAIIAPVSVLILVLIISGWTKKGRD
                     QQIISVPQFYPIEGQSPAEVGYIIDGVINNKDITSLLFYWADKGYLEITEKRWSYEFT
                     RLTVPVFENDYERYMFNKLFEYGDGDTVTTDDLKDNFYSAVPNIKSKLKQVFKGRKEL
                     VSKDSLKARMEYFMLALVPFAVFLAVLFLEFFDMGFLIAFILIYGGFYATLGFMYVNA
                     ENKWNIIGKFRKLLYAVAVVIMLILTTVIGHAIITDGYYYFNIRTYSIAYFIVAFVTA
                     ISLAYCAAMKKRSEYGQYVLEYNLGFKDFIANAEMDKLKTLIDEDPKYFYNILPYAIV
                     LGLERKWGRKFENIITQPPDWYHGSSMRAFSTMHMLSGMNSCLARTTSSMVQAPSSSS
                     GGGFSSGGFSGGGGGGGGGSSW"
     gene            complement(2609..3160)
                     /locus_tag="PKJ65_02325"
     CDS             complement(2609..3160)
                     /locus_tag="PKJ65_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ40708.1"
                     /translation="MILGYVVLGVILLVAVYFIATYNAFIRLRNKYEEAFAAIDVFCK
                     KRYDLIPNLIETIKGYAKHEEQTFIKVVEARAKAMQSKTVAESQANETALEGALKSLF
                     ALAESYPDLKANQGFLDLQAQLTTIEGELAGSRRYYNAIVQSFNTKTQTIPSSFVASI
                     CRFEKQPYYEVAEEARENVKVSF"
     gene            3381..3572
                     /locus_tag="PKJ65_02330"
     CDS             3381..3572
                     /locus_tag="PKJ65_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40709.1"
                     /translation="MDEYIDKITDLTDRYVSSITVFTTEKSDPYNKEYIAPSAISIRK
                     TLIITSVCFNPFSRNYRSA"
     gene            3630..>4694
                     /locus_tag="PKJ65_02335"
     CDS             3630..>4694
                     /locus_tag="PKJ65_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40710.1"
                     /translation="MYEPVSFCMSVIHASYVYRLAEKYTKELFTHDTHIGKDLQRAAL
                     DYIEGAKPLIQSLKEDTVFKYAALIHCARDMEDNNLKQCVKEMKERVRDEEYFSVQYL
                     QALCDIHNETKDKQYLKKALNVMTSVLKLTEPDNTVIDLKEEKNNLDKRSATVYLWDY
                     IDSLLYLAFTDNNESLCNVLSYVFDLIDENRCPKSSDNVLLLPVFTLLNKKLFSIILH
                     KTTKSKALSAFNGFVGDNVYRHGTGTYISQTVSKEQDTFYTMQCGNLKVRLRFNTTYF
                     GPRGRFRSDVLEETDTGLRLRYIREWGYWLPLEDRSVPAIIGQDVNKVDRTMTNLQHI
                     DMQADIKFLDNGADITISIKG"
BASE COUNT         1370 a         1021 c          856 g         1447 t
ORIGIN      
        1 agttcttctc tgacgcctct tcttcttccg tcaattgcag gtcttatgcc gactttaggc
       61 aaagtgcctt tcagtctttc aacgttgttt ttctttgtca tttctcatac tccttccata
      121 tatgttaaat caactattta ttaccagctg gaaccgccac cgccaccgcc gccgcctccg
      181 gaaaatcctc ccgatgagaa tccgcctccg ctgctggagc tgggtgcctg aaccatggat
      241 gatgtcgtcc gggcaagaca gctgttcatt ccggataaca tatgcatcgt ggaaaatgcc
      301 ctcatactgg atccgtgata ccaatcgggc ggttgcgtaa ttatgttttc aaacttcctg
      361 ccccattttc tttccagacc caatactatt gcataaggca gtatattgta aaagtatttc
      421 ggatcctcat caataagcgt ttttaacttg tccatttcgg cattggctat aaagtccttg
      481 aatcccagat tgtactccag aacatactgt ccgtattccg aacgtttctt catcgcagca
      541 caatatgcca gcgatattgc ggttacaaaa gcgacaatga agtatgctat cgaatatgtc
      601 ctgatattaa agtaatagta cccgtcggta attatcgcat gaccgattac ggttgttaaa
      661 atcaacatga ttaccaccgc taccgcatac agtaattttc tgaatttacc gattatattc
      721 catttatttt ccgcattaac atacatgaaa ccgagggttg cataaaaacc tccgtaaatc
      781 agtataaacg ctattaaaaa ccccatatca aagaactcca gaaacagcac tgccagaaat
      841 accgcaaaag gcaccaaagc cagcataaaa tattccatgc gggcttttaa tgaatctttg
      901 gaaaccagtt cttttcttcc tttgaaaacc tgtttcaatt tgctctttat attcggaacg
      961 gcggaataga aattatcctt caaatcgtca gtggtaaccg tgtcaccgtc tccgtattca
     1021 aacagtttgt tgaacatata ccgctcatag tcattttcaa ataccggcac ggtcagtctt
     1081 gtaaactcat agctccaacg cttttccgtt atctccagat accccttgtc cgcccagtag
     1141 aacaataaag aggtaatatc tttgttattg ataactccgt cgattatata gcccacttcc
     1201 gcaggagact gtccttcaat gggataaaac tgcggtacgg agataatctg ttggtctctt
     1261 cctttttttg tccagcccga tattatgagt accagaatca gcacggatac cggagcaatt
     1321 atcgctatga ccggaatcct tacacccgct gcaggacgtt cgtttacaaa atatccttca
     1381 ggaagttcca gcgcaagtgt aaccgattga taggaagaca gaccggacaa ttgtcctttt
     1441 acggtattac ccgttacggt atatccggta taccttgtgt cactatacca atccccgtat
     1501 gtaatactta ttttctcttt gtcaaacggt ttaggcatat tcaccgtgaa agtgaccaga
     1561 taaacatcgc agtcccagtc tcttcccagt atattatggt aaatcttatc gtatgcttct
     1621 tccaagtcgt cgccgtagtc gtaatcatag gttattttgt atttaacctc gccctcgaca
     1681 attttatcgg cgtcgcctat ccgaatggat acatattctc ttgcatgctc cactacatat
     1741 gtcttgtcca catatatgtt gtctacataa actctcttgc cttcgaaaac ggtgggaaga
     1801 gtacgtatta taccgtgctt gccgtaggcg gtaaaatcag ccgtaatatt ttccgttata
     1861 tgcatgacat tgttttcacc tacatctata acggtttcat aatgcgagat atgatatccg
     1921 tgtctgtcat acaccgttac gggacgttca tttacaaaat atccttcggg cagttccagt
     1981 gcaagagtaa ttccctggta tgcggacagg tcggtcagtt gtccttttat gctgtttccc
     2041 gaaacggtat atcccgtata tttactatca ctgtacctgt ccccgtatgt aatgcttatt
     2101 ttctctttat caaacgattt gggcatattt acagtgaatg tgactttata aatatcacag
     2161 tcccagtcgg ttcccaatat gttatggtaa atcttatcgt aagcatcttg ttggtcatca
     2221 ccgtaatcgt aacgataagt tattttgtat ttaacctcac ctttaacggt tttgtcggca
     2281 tcacctatac gtatggaaac ataatccgct tcagtactca ctacataggt tttatcaaca
     2341 tatatgtcgt cgataaaaac tctctgattt ccgaaaacgg tgggaattgt acgtattata
     2401 ccgtgcttgc cgctttcggt aaaaacagca gcaatattct ccgttatgta cattacattg
     2461 tcttcaccta catctattgc aatgtcataa tcggaaatat ggtatccgtt cacatcatac
     2521 gcaaaaaccg gtaaaaaagc gcatatcagt aaaactgaca gcgctattga cactatactt
     2581 ctataaaaac ctgttttttt catacctctt aaaagctcac ttttacgttt tcgcgtgctt
     2641 cctctgcaac ttcatagtaa ggctgttttt caaatctgca tatacttgcc acaaaggaac
     2701 tggggattgt ctgtgtcttt gtattgaaag actgtacaat tgcattgtaa tatcttctgg
     2761 agcctgcaag ctctccttca atagtggtca actgtgcctg caaatccaaa aacccctgat
     2821 tggcttttaa atccggatag ctctccgcca gtgcgaaaag acttttaagg gcaccttcca
     2881 gtgccgtctc atttgcctgt gattcggcaa cggttttact ttgcattgct tttgctctgg
     2941 cttccactac tttgataaaa gtctgttctt catgctttgc atatcctttt attgtttcaa
     3001 taagattggg tatcaaatca taccgctttt tacagaacac atcgattgcg gcaaatgctt
     3061 cctcatattt gttccttaag cgtataaatg cgttatacgt agcgataaaa tacactgcta
     3121 caagcagtat aactcctaat acaacataac caagtatcat ccatcccatt attattacct
     3181 ccgtttgatt ttattatata ttaacaacta taatcataca aatgaatttt tgataaagtt
     3241 atatgctctt ttactattac aagttattaa gatgcggaca tgccgaaata tatgcaaaat
     3301 tttcctttag agttggcaca tccctttgat gttgacgaat aaatcgcttg tgctataatc
     3361 accttgaaag tgagtaacct atggatgaat atatcgataa aataaccgat cttaccgaca
     3421 gatatgtctc gtcaataact gtttttacaa cggaaaaatc cgatccttac aataaagaat
     3481 acattgctcc ttcggcaata tccatcagaa aaacattgat tataacaagt gtttgtttta
     3541 atcctttttc aagaaattac cggtcggcat gactgttaaa agcagcttcg gaatatctta
     3601 acaatgcaat tgcacttgtc aataaaggca tgtacgaacc tgtatctttt tgtatgtcag
     3661 tcatacatgc atcgtatgta tacagattgg cagagaaata tacgaaagaa ttgtttaccc
     3721 acgatacaca tataggcaaa gacttacaga gagcagcttt agattatata gagggtgcta
     3781 aaccgctgat acaaagtttg aaagaagata ccgttttcaa atatgcggca ctgattcact
     3841 gcgcaagaga tatggaagat aacaacctga agcaatgcgt caaagaaatg aaagaaaggg
     3901 tgcgggatga agaatatttt tccgttcaat atttgcaggc actttgcgac atacacaacg
     3961 aaaccaaaga caaacaatac ttaaaaaagg cattgaatgt tatgacatcg gtgcttaaac
     4021 ttacggagcc ggacaatacc gtaatagact tgaaagaaga aaagaataat ctcgataaaa
     4081 gaagtgcaac tgtttatctt tgggactata tcgattcttt gctgtatctg gcttttacag
     4141 acaacaatga gagtctctgc aatgttcttt cctatgtatt tgatttaatt gatgaaaacc
     4201 gttgtccgaa aagcagtgac aatgtacttt tactgcctgt ttttactttg ttaaacaaaa
     4261 agcttttcag catcatttta cataaaacaa caaaaagcaa ggccttatcc gcatttaacg
     4321 ggtttgtcgg cgataatgtt tatcgtcacg gaaccggtac gtatatatcc cagacagtat
     4381 ccaaagagca ggatactttc tacaccatgc agtgcgggaa cttaaaggta cgtctccggt
     4441 tcaataccac atacttcggg ccgagagggc gtttcagaag tgatgtgctg gaagaaaccg
     4501 ataccggatt gaggttgagg tatataaggg agtgggggta ttggctgccg ctggaagacc
     4561 gatcggttcc tgcgattata ggtcaggatg tcaataaagt cgacagaaca atgaccaatc
     4621 tgcaacatat agatatgcag gccgatataa agtttctgga taacggagcg gatataacca
     4681 tatccattaa ggga
//
LOCUS       DAOPWC010000068         4633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_18684_length_4633_cov_4.112276, whole genome shotgun sequence.
ACCESSION   DAOPWC010000068 DAOPWC010000000
VERSION     DAOPWC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18684_length_4633_cov_4.112276"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PKJ65_02340"
     CDS             <1..1146
                     /locus_tag="PKJ65_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40711.1"
                     /translation="TNISYQNTEMGPVNANQWIYAEISGAVESWDRLEPSVQKPYMLH
                     TNLIGHGAVNFTANDIGMAKTRVQNGASWLSTGSIYTFKTNGYYATFKVPSMGVPDVN
                     LNAAFTEERLTYSAADVHKKGYINVNFTIDKPLKEIDYIKLSVQGGNSIILQPHERNG
                     QLELDIPIQSVMPARYKVRVLIEVKSIFNDVHITREYCSVDLYKEETPYNPTDPVQPE
                     EIISDLPEDIPYQANYDIHVTDISLTGYWNIWHLNERFTSLERVRLNVAVAGRVTKLS
                     VIFSEELKSRTYRDKYGNTYDYKELLGSYDMCPNDICYEGLWENGYTLNTEYILPLCP
                     DTVDSSGNRIKDKYFIKIIAENENKEHTKEYLIDITGNIYDFLYVQP"
     gene            1170..1748
                     /gene="purN"
                     /locus_tag="PKJ65_02345"
     CDS             1170..1748
                     /gene="purN"
                     /locus_tag="PKJ65_02345"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNZ40712.1"
                     /translation="MNICVFASGGGSNMQAVIDACKNRKINAKVVLVISNNSDAYALE
                     RAKKEKIDNYHVSEYKYKESFIDKLLELLDKYEINLVLLAGYMKKLPKEIVERYKVLN
                     IHPALLPKYGGRGMYGERVHKAVLEAKEKESGCTVHVVDEHYDNGKILNQIKVSVLED
                     DTVETLAKRVLEQEHIIYVDTVKKILTKEIEI"
     gene            1755..2000
                     /locus_tag="PKJ65_02350"
                     /pseudo
     CDS             1755..2000
                     /locus_tag="PKJ65_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
     gene            complement(2008..3393)
                     /locus_tag="PKJ65_02355"
     CDS             complement(2008..3393)
                     /locus_tag="PKJ65_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ40713.1"
                     /translation="MTDNNIKDFRQDIIKQITKARVAKGLTQSQLAELLGMHRSAISR
                     LESGSHSPSLDLLLQVASVLELKIELTTPSTKVLSHMKNNYELRLFDKVLLTFSLEKR
                     GIEGLTAHIISIDKEQLHLLPLGLELSDKGILRWLEKRVIPKNRTFVKEILKTLNLSQ
                     GDTKGIIDICKGLSLNDSFWVVPEGFEGSFAQYNLYENRFSEILSLVAYTGIEQSHGG
                     FTTSPELTTAGMLPKAWRLIDNDGIYLYKGGTFGAANTGNEPYSEYYACQIAQTMGLN
                     AVQYELENWKGILASKCKLFCDIDTAFVPVGRIVTSGGLKAVLKYYSSIGEDFLQAVH
                     SMLVFDAVIYNEDRHFGNFGVLRDNHSGALIAPAPLFDHGLSLFNYAMPKDIKNLDQY
                     AKTRLPAYPDVTFEDICREVAGKAQAQQLRRLIGFTFKRHPSVNWPEERLTAIEKHLQ
                     KRVRQLLGMVK"
     gene            complement(3504..3668)
                     /locus_tag="PKJ65_02360"
     CDS             complement(3504..3668)
                     /locus_tag="PKJ65_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40714.1"
                     /translation="MLHFSKAELNRQANALGFVRDTFEKICRLADVLGFMENNKKPRK
                     VFKVGIAFTI"
     gene            complement(3655..4419)
                     /locus_tag="PKJ65_02365"
     CDS             complement(3655..4419)
                     /locus_tag="PKJ65_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNZ40715.1"
                     /translation="MKYYENMVEMGCFSRSELAKALQLGDNTAATILQQYQKKGYIER
                     VRHNFYVVISIENKQPILSRYGIGSRLFPDASVSHHSAFEVYGYANQVFYEVYVMTGS
                     RFKDFEYDGITFRRVSPKETVQTEIIGGVRVTGVEQTVIDSINDVDKIAGLEELLRCL
                     DLIPTLNEEKLLFTLNEYQNGFLYQKTGFILKHFRESYGLSDAFFRICADRIPKSDRY
                     LTKEHIGYVYHPEWRLMAPKDINTVINKGVDYHAAL"
BASE COUNT         1454 a          909 c          954 g         1316 t
ORIGIN      
        1 accaatatat cttatcagaa caccgaaatg ggtcccgtta atgctaatca gtggatatat
       61 gccgaaatat ccggagcggt cgaatcgtgg gacaggctgg agccttcggt gcaaaaaccg
      121 tatatgctgc acacaaatct tatagggcac ggagcggtga attttaccgc aaatgatata
      181 ggtatggcta agacaagggt gcagaacggc gcatcctggc tcagtaccgg ttccatctat
      241 accttcaaaa ccaacggata ttatgccact ttcaaggttc cttcaatggg tgttcccgat
      301 gtaaatctga atgccgcttt tactgaagaa aggcttacct attccgctgc cgatgtacat
      361 aagaaaggat atatcaatgt aaacttcacc atagacaaac ctcttaagga aattgactat
      421 ataaaactgt cggtacaggg aggtaacagc attattctgc agcctcatga aagaaacggg
      481 caactggagc tggacatacc gattcagagt gtcatgccgg caaggtataa ggtgagggta
      541 ttaatagaag ttaagagcat atttaacgat gttcatataa caagagagta ttgttctgta
      601 gacctttata aagaagagac tccgtataac ccgactgatc ccgtacagcc ggaggaaata
      661 atatccgacc tgccggaaga tattccgtat caagcgaatt atgatattca cgttaccgac
      721 atttcattga cgggttactg gaatatatgg catctgaatg aaagatttac ctcactggaa
      781 agagttcgct taaatgtagc ggttgcaggc agagtaacaa aactgtcggt catattcagt
      841 gaagaattga aaagccgtac ataccgggac aaatacggaa acacctatga ttataaagag
      901 cttttaggca gttatgacat gtgcccgaat gatatatgtt atgaaggatt atgggagaac
      961 ggctacacat tgaatacgga atatatactt cctctgtgtc ccgataccgt tgattcttcc
     1021 ggtaacagaa taaaggataa gtattttata aaaataatag ccgaaaacga gaataaggag
     1081 catacaaagg aatacctgat tgatattacc ggtaacattt atgattttct gtatgtgcag
     1141 ccatagcata tgatgatata atttccatga tgaatatttg tgtattcgcc tcgggcggag
     1201 gttccaacat gcaggcggta atagatgcct gtaagaacag gaagatcaat gcaaaagtcg
     1261 tactggttat ttccaacaac agtgatgcct atgctttgga gcgtgcaaaa aaggaaaaga
     1321 tagataatta tcatgtaagc gagtacaaat ataaagaaag ttttattgat aagttgctgg
     1381 aattgttgga taagtatgag attaatctgg tactgcttgc aggctatatg aagaaattgc
     1441 ctaaagagat tgtggagaga tataaagtgc tgaatatcca tccggccctt cttcccaaat
     1501 acggaggccg gggcatgtac ggagaaagag tacataaagc ggtactggaa gccaaagaaa
     1561 aagaatcggg ctgtaccgtt catgtggtgg atgaacacta tgataacggt aagatactaa
     1621 atcagataaa agtttccgta ctggaagacg ataccgtgga gacattggca aaaagggtat
     1681 tggaacagga acatatcata tatgtcgaca ccgtaaagaa aatattgaca aaagaaatag
     1741 agatatgagg agatatgtac agatataaaa caaaaggaac atgttcaacc gagataactt
     1801 ttgagataca ggaaggaaaa ctgaaggatg tgaaatttac ggacggatgc agcgggaatc
     1861 ttcagggtat aagcaaattg cttgagggta tggaagcata gaaagccgta agcctgttga
     1921 aaggcattaa atgcggttat aaagaaacat cctgccccga tcagttgtcc atagcgataa
     1981 gtaaggtgtt ggaacactga atccttttta ttttaccatt cccaacagtt gtcgtacccg
     2041 tttttgcaga tgcttttcaa ttgccgtcaa tcgctcttcc ggccagttaa cggacggatg
     2101 cctcttgaag gtaaaaccga ttaaacgacg caactgctgt gcctgtgctt ttcccgcaac
     2161 ttctctgcaa atgtcttcga atgttacatc cggataagcg ggtagacggg ttttagcata
     2221 ttggtcaagg tttttgatgt ctttaggcat agcataatta aacagtgaaa gtccgtggtc
     2281 aaacagaggt gcaggggcaa tcaatgcacc tgaatgatta tctcttaaga ctccgaaatt
     2341 tccgaagtgt ctgtcctcat tgtagattac cgcgtcaaat accagcatgc tgtgtaccgc
     2401 ttggagaaaa tcttccccga ttgaagaata gtattttaat accgctttca aaccgcccga
     2461 tgttacaatg cgtcctaccg gtacaaaagc ggtatcaata tcacaaaaca acttgcattt
     2521 tgaagctaaa atacctttcc agttctccaa ttcatactgc acggcattta gacccatggt
     2581 ctgtgcaatt tggcaagcat aatattcact gtaaggctcg tttcccgtat tagcggcacc
     2641 gaatgtgcct cccttataca agtaaatacc gtcattatca ataaggcgcc atgctttagg
     2701 caacattccg gcagtagtca gctccggcga ggttgtgaaa cctccgtgac tttgctctat
     2761 acccgtatag gcaaccaaag aaagaatttc cgaaaaacgg ttttcataga ggttatactg
     2821 agcaaaggaa ccttcaaagc cctccgggac tacccaaaaa ctgtcgttaa gcgacaagcc
     2881 cttgcaaata tcaataatac ctttagtatc accttgagac agatttaaag ttttcaagat
     2941 ctctttcaca aaggtacggt ttttaggaat cactcgtttc tccaaccatc tcaaaatgcc
     3001 tttatcagac agttccaatc caagcgggag taagtgaagt tgttccttgt caatagaaat
     3061 aatatgtgct gtcaatcctt caattcctct tttctcaaga gagaaagtga gtaatacttt
     3121 gtcaaagagc cgtaactcgt agttgttttt catatgggaa agtactttcg ttgaaggtgt
     3181 tgtcagttca attttcagct ccaaaaccga agccacttgt agtaaaaggt ccaatgaagg
     3241 actgtgactt ccgctttcca agcgtgatat agcggaacgg tgcataccta aaagttcagc
     3301 taactgactt tgtgtgagac ctttagcaac ccgtgctttt gtaatctgtt tgataatatc
     3361 ctgacgaaag tctttaatat tattgtccgt cattaaaatg cctcctgctc tattattgca
     3421 aaatgtaaca aatttgtcaa caaattacaa gaaacattta ataacagttc tacgtggtaa
     3481 caaatatgta gacaattatt tgattaaatt gtaaatgcta ttcctacctt aaaaactttt
     3541 cgtggttttt tattattttc cataaaacca agcacatctg caagacgaca aatcttttca
     3601 aatgtatctc ggacaaaacc aagagcattg gcttgtctgt tcagttctgc tttactaaag
     3661 tgcagcatga taatctactc ctttatttat caccgtattt atatcctttg gtgccataag
     3721 tctccattcc ggatgatata catatcctat atgttccttt gtgagatagc ggtcggattt
     3781 aggtatacga tctgcacaga tgcggaagaa agcatccgat agaccgtaag actcgcgaaa
     3841 gtgtttcagg ataaaaccgg ttttttggta gagaaaacca ttttgatatt cattcagagt
     3901 aaatagcagt ttttcttcat ttagggtagg tatcagatca aggcagcgga gaagctcctc
     3961 cagtcccgca atcttatcta catcattgat actgtcgata accgtctgtt ctactcctgt
     4021 aacacggaca cctcctatta tttccgtttg cacagtctct ttaggtgaaa cacggcggaa
     4081 agttattcca tcgtattcaa agtccttgaa acgactgcct gtcataacat aaacctcata
     4141 aaatacttga tttgcatacc catatacttc aaaggcacta tgatgtgaaa cggacgcatc
     4201 gggaaatagg cggctgccta ttccgtaacg cgataagatt ggttgcttgt tctctatgct
     4261 tatcacaacg taaaagttat gcctgacccg ttctatatat ccttttttct gatattgttg
     4321 taatatggtg gctgctgtat tatcccccaa ttgtaaagcc ttagcaagct cactacggga
     4381 aaagcaaccc atttctacca tattctcata gtatttcaaa agaatatacc tcctgtattt
     4441 ttaattattg cacaaaaaga cttaaacagc aatatagttg tgcaattatt aaaaagggga
     4501 ggaaataaaa aaagcggtaa ttttgccgct attctataac cgttaaaaca tcttccaagg
     4561 attttctttt cttcggacgg agtttgttgg agtcgtcttt atagcctacc gctataacca
     4621 gccttatcct gtc
//
LOCUS       DAOPWC010000069         4621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_18739_length_4621_cov_3.735874, whole genome shotgun sequence.
ACCESSION   DAOPWC010000069 DAOPWC010000000
VERSION     DAOPWC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18739_length_4621_cov_3.735874"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /gene="rbfA"
                     /locus_tag="PKJ65_02370"
     CDS             complement(<1..320)
                     /gene="rbfA"
                     /locus_tag="PKJ65_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ40716.1"
                     /translation="MKARRVEQLNSEFKREISSILADEIKDHRLTAIVSVINVNVTRD
                     LSYADIHVSMIGTEQQKKDSFDAILSSAGYIRKLLGQRMKIRYIPQLRFKYNDYIEEG
                     IRIS"
     gene            complement(317..3205)
                     /gene="infB"
                     /locus_tag="PKJ65_02375"
     CDS             complement(317..3205)
                     /gene="infB"
                     /locus_tag="PKJ65_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ40717.1"
                     /translation="MGKRVNELAREIDKKPSEVLRKLEQMGIKSNVNTILTDEQVQMI
                     YDIYRIKNPAAGMKVRTTVVTSSDTATPVKTVVVKAPVKQKTKTEKDAADEKPMVVVV
                     KTTKGGETKSTIKHIQKEPQKTPETKKEVPQKPQEKKVVVVKTPEVTVPRKETVAETP
                     KTVKAEETQIRKPQIAIPQKPEQKEDVQKTTEKEDIVKKEEKKEKTYIEQKAYKDKTY
                     IPKEKTDKPYTPRDSRPDKGGYHQRDSRTDRPYTPKPADKKPGFARTDSHDGRTKPTV
                     RKPVDRDSGDDSAARIEKPRVIKKEYIKKDQDKVLNKETKKETAVAKSIASKQTKYEK
                     REILTPKGAIDTFMLTDDELFSTFYRDSGVRKKRKKRDANQPKIEVLKFVKLPPEMSV
                     KEFAEAIKKTGSDVLSKLLDMGSNIMSINENIDFDTAALIADEFGITCEKENIITEED
                     ILFDDDKECDPKDLVERSPVVVVMGHVDHGKTSLLDYIKNSNVAASEAGRITQHIGAY
                     RVKIGERYITFLDTPGHEAFTAMRKRGADVTDIAIITVAADDGIMPQTIEAINHAKAA
                     NVNIIIAINKIDLPTANPDRVMQQLMDHNLVATKYGGDIECIPVSAKTGEGIDTLLDT
                     ILLSADILELKANPTRQAKGIVLESRVERAGTVTTLLVQRGTLKKTDYIISGTSVGNI
                     RALTDDRGNNIESAGPSMPVEILGLDAPPEAGEIFYAVDDERTAKSLAERRKYERQIA
                     KTAHKLTTLDDLYEQIKAGNIKDLNLIVKADVQGSVEALTDSFKKLSTDQVKVHVIHA
                     AVGTVTETDVSLAEVSNSIIIGFNVRPLGNVTDLAKEASVDIRLYSVIYDAIEDIKKA
                     MAGMLDPVTKEVVYGHAQVRETFKITGVGTVAGCMVLDGKILRNTNVRLVRDGITVFD
                     GALSSLKRFKDDVKEVAAGYECGMSIEKFNDLKINDIIECYKTEVIKK"
     gene            complement(3206..3493)
                     /locus_tag="PKJ65_02380"
     CDS             complement(3206..3493)
                     /locus_tag="PKJ65_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNZ40718.1"
                     /translation="MIGLAIKAGAAATGTAGCITKIKNNRIMLVFIADDCSKNTREKI
                     LSAVKNKNIEISEMFSKEQLGSIIGKEEISVIGISDKNFAKGISDKITEEN"
     gene            complement(3504..3773)
                     /locus_tag="PKJ65_02385"
     CDS             complement(3504..3773)
                     /locus_tag="PKJ65_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNZ40719.1"
                     /translation="MKNKKIPMRMCLSCRIMKPKKELIRIVLTQDNKAVMDLTGKRNG
                     RGAYICADLQCLFKAKKAKSIERTFSIGQDDTIYGEMEEYINRKE"
     gene            complement(3763..>4621)
                     /gene="nusA"
                     /locus_tag="PKJ65_02390"
     CDS             complement(3763..>4621)
                     /gene="nusA"
                     /locus_tag="PKJ65_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ40720.1"
                     /translation="IAAQTTKQVILQRIKDAERIKILDMYENKLKEIVYGVVRRVEND
                     TVYLDLGNSEAIMPKKEQVPSERYGVNFKFKAFVYDVKKTSKEPKIFVSRTHPDLIKR
                     LFEMEVPEIQSGIVEIKDIAREPGARTKIAVYSNDPNVDATGACIGHKGIRVSNVVEE
                     LRGEKIDIIEWSPNPAQYISSALSPADVSRVDVIEVENAEGESEIKARVTVPDNQLSL
                     AIGKEGQNARLAAKLTNWKIDIIPETELRRIIEEQLLSDDLLAKDTKPVHELDALFDH
                     PTEKEKDEE"
BASE COUNT         1043 a         1077 c          766 g         1735 t
ORIGIN      
        1 ttactaatcc ttattccctc ttctatataa tcgttgtact taaaccttaa ctgtggaata
       61 tatcttatct tcatcctttg ccccagcaat tttcgtatat atcctgccga acttaaaatc
      121 gcatcgaaac tgtctttttt ctgctgttcc gttcctatca tgcttacatg tatatcggca
      181 taggataaat ctcttgtaac attgacattg ataaccgata caatagccgt aagtctgtga
      241 tcttttattt catcggcaag tatagaggat atctctcttt taaactcact gttcagctgt
      301 tccactcttc ttgccttcat ttcttaatca cctctgtttt gtagcattcg attatatcat
      361 taatctttaa atcattgaat ttctcaatgg acataccgca ttcgtaacct gcagctactt
      421 cctttacatc gtctttaaat ctcttaagtg aactgagagc tccgtcaaac accgtaattc
      481 cgtctcttac aaggcgaaca ttagtgtttc tgagtatttt accgtccaga accatgcacc
      541 cggctaccgt tcccactccg gttatcttaa atgtctctct tacctgtgcg tgaccgtata
      601 ccacttcttt tgtaacggga tccagcatac ccgccattgc ctttttaata tcttcaattg
      661 catcatatat gacgctgtaa agccttatat ccaccgaagc ttcttttgcc agatcggtta
      721 cattacccag cggtctgacg ttaaaaccga tgatgatgga attggatact tcagccaaag
      781 atacatcggt ctccgttacg gtgcctaccg cggcatgaat aacatgtact ttaacctggt
      841 cggtggaaag ttttttaaac gaatccgtta aagcctctac cgaaccctga acatccgctt
      901 taactataag gttgagatcc ttgatatttc ctgctttaat ctgttcatat aagtcatcca
      961 gagtggtaag tttatgtgcc gtttttgcta tctgcctttc gtattttctt ctttccgcca
     1021 atgattttgc ggttctctca tcatctacgg catagaatat ctcgcctgcc tcgggcggtg
     1081 catccagacc gagaatttcc acaggcatag aaggtccggc agactctatg ttgtttcctc
     1141 tgtcatccgt aagtgccctt atatttccca ccgatgttcc ggagataata taatcggtct
     1201 ttttgagagt acctctctga accagaagcg tagttaccgt tcccgctctt tcaactcttg
     1261 actccagtac gatacctttt gcctgtctgg taggatttgc ttttaattcc agaatatcgg
     1321 ctgataacag tattgtatct aacagagtat ctataccttc accggttttt gcggaaaccg
     1381 gaatacattc gatatcaccg ccgtattttg ttgcaacaag attatgatcc ataagctgct
     1441 gcattaccct gtcgggattt gcggtcggaa gatcgatttt gtttatagct ataataatat
     1501 ttacattcgc ggcctttgca tgatttatag cttcaatggt ctgcggcatt attccgtcat
     1561 cggcggcaac ggttattatt gctatatcgg tgacatccgc tcctcttttt ctcattgcgg
     1621 taaaagcttc atgaccggga gtatccagga aagtgatata cctttcacct atttttactc
     1681 tgtatgcacc gatatgctga gtaatccttc cggcttcgct ggcagcaacg ttggaatttt
     1741 taatgtagtc caataaagat gtttttccgt ggtcaacatg ccccataaca acaacaacgg
     1801 gagatctttc cacaagatcc ttcggatcac attctttatc atcgtcgaat aaaatatctt
     1861 cttcggtaat aatgttttcc ttttcgcatg ttataccgaa ctcgtcggct attaatgcgg
     1921 ctgtatcgaa atcgatattc tcatttatac tcataatatt tgatcccata tccaacaact
     1981 tggacagcac atcggagccg gtctttttta tggcttcggc aaattcctta acggacattt
     2041 ccggcggaag ttttacaaat ttgagtactt cgattttagg ttgatttgca tctctcttct
     2101 ttctcttttt tcttactccg ctatctctgt agaatgtact gaacaattca tcatctgtaa
     2161 gcataaatgt atcgatggca cccttgggag tcaaaatttc tctcttctca tatttggtct
     2221 gttttgaagc tattgatttg gcaacggctg tctctttctt ggtttcttta ttaagaacct
     2281 tatcctgatc cttcttaatg tattctttct ttataactct cggtttttca attcttgcag
     2341 cggaatcgtc accgctgtct ctgtcaacag gttttctgac agtaggtttt gttctgccgt
     2401 catgtgaatc ggtacgagca aatcccggct tcttatcggc aggcttaggc gtataaggtc
     2461 tgtctgtccg gctgtctctt tgatgatatc ctcccttatc cggcctgctg tctctgggag
     2521 tataaggctt atccgttttt tccttaggaa tatatgtctt gtctttatag gctttctgct
     2581 ctatatatgt tttttctttc ttttcttcct ttttgactat atcttccttt tccgttgtct
     2641 tttgaacatc ttccttttgt tcgggttttt gcggtatagc tatctgaggt ttcctgattt
     2701 gcgtctcttc cgctttgact gttttaggcg tttctgcaac cgtctctttc cgtggaacgg
     2761 tcacttcagg agttttaaca acaacaactt tcttttcctg gggtttttgg ggtacttctt
     2821 ttttagtctc cggagttttt tgaggttctt tctgtatatg ctttattgtg gatttggttt
     2881 caccgccttt tgttgttttt acaaccacaa ccataggttt ttcgtcagct gcatccttct
     2941 ccgtttttgt tttctgtttt acgggagctt taacaacaac ggtcttaacc ggtgttgcag
     3001 tatcagacga agttacgacg gttgttctta ccttcattcc tgccgcagga ttctttattc
     3061 tgtagatatc ataaatcatc tgtacctgtt cgtcggttaa gatggtattg acattggatt
     3121 tgatacccat ctgttctaat tttcttagaa cttcactcgg ttttttatct atttctcttg
     3181 ctaactcgtt tactcttttc cccatttagt tctcctcggt tatcttatcg cttatgcctt
     3241 ttgcaaaatt cttatcgctt attcctatca ctgatatctc ttcttttcct ataatactgc
     3301 ctaactgctc tttggaaaac atctcggata tctctatatt cttattcttt actgcactca
     3361 atattttttc tctggtattc tttgaacaat catcagctat aaaaacaagc attattctgt
     3421 tgttctttat ctttgttatg cagccggctg tacctgttgc agccgctccg gcttttattg
     3481 ccaaccctat cattttgaga atattactct tttctattta tatattcctc catctcaccg
     3541 tatattgtat catcctgtcc tatggaaaat gttctctcaa tgctttttgc tttcttcgct
     3601 ttgaaaagac actgtaaatc agcacatata tatgctcccc gcccgtttct cttacctgtc
     3661 aaatccataa ccgctttatt atcctgtgta agcacaatac gaatcaactc tttcttgggc
     3721 ttcattattc tgcatgataa acacatcctc atgggaatct ttttattctt catctttctc
     3781 cttttccgtc ggatgatcga ataaagcatc caactcgtgt accggttttg tatctttagc
     3841 aagtaaatca tccgataaaa gctgttcttc aataattctt cttaattcgg tttcgggaat
     3901 aatatcaatt ttccaatttg taagctttgc tgcaagtctt gcattctgtc cttctttacc
     3961 tattgccaat gataactgat tatccggtac ggtaactctg gcctttatct cgctttcgcc
     4021 ttctgcattc tctacttcga taacatccac tctggaaaca tccgcaggac ttaaagccga
     4081 cgaaatgtat tgggcgggat tgggactcca ttcgattata tctatctttt ctcctcttaa
     4141 ttcctccact acattggaaa cccttatgcc tttatgtccg atacatgctc ctgtggcatc
     4201 cacgttgggg tcattggaat aaaccgctat tttggttctt gcaccaggtt cacgtgctat
     4261 atctttaatt tccactatac cgctctgaat ctccggtact tccatttcaa acagacgttt
     4321 aatcaaatcg ggatgtgttc tggaaacaaa tattttaggc tctttactgg ttttcttaac
     4381 atcataaaca aacgctttga acttgaaatt aactccgtaa cgttcactcg gaacctgttc
     4441 tttcttgggc ataattgctt ccgaattgcc taaatcaaga tatacggtat cattttccac
     4501 tcttcttact acaccgtaca caatctcttt gagcttattc tcatacatat ccaatatttt
     4561 tattctctct gcatctttta ttctctgtaa aatgacctgt tttgtggttt gtgcggcaat
     4621 a
//
LOCUS       DAOPWC010000070         4562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_19036_length_4562_cov_3.571777, whole genome shotgun sequence.
ACCESSION   DAOPWC010000070 DAOPWC010000000
VERSION     DAOPWC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19036_length_4562_cov_3.571777"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..908)
                     /locus_tag="PKJ65_02395"
     CDS             complement(168..908)
                     /locus_tag="PKJ65_02395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40721.1"
                     /translation="MNYHKITKQYYTKWLGISSESFETRGMHFIYSKERNITQYGYSH
                     PFDIYVWLQPGKLIVSYGDKASEKINELKQKMNVAMSSAKVKELLSDTFGMPITQSIK
                     YVYRETNAVSCAVILSCSDYPSYLEFFRKAHLGCNNTDWLREYFDEMVQYGLCVGIFE
                     NSKLVSCSDAPGMPYMQETVQEIGVNTLPEYRGRGYASEVCIKCAKSIIKLGKCPIWS
                     TEIDNAASQKLADKVGFVKFAETLAMTL"
     gene            complement(1024..1887)
                     /locus_tag="PKJ65_02400"
     CDS             complement(1024..1887)
                     /locus_tag="PKJ65_02400"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ40722.1"
                     /translation="MNSVLDSMMSKYPVATSDAEAKNAVKQALQEITLCGLGRSDFYQ
                     NAAFNGGTALRIFHGLQRFSEDLDFSIDNEKIDTFVLDKYVGCINNELTSLGIPATFE
                     VDSKSGFIQRGYIKGNCREILNCFGTEEVFTGRVPANEVLKIKLEADAAALSAASYET
                     KYLLQPYPTPVRLYDKGTLFAGKVSAVLSRSWKSRVKGRDLYDYLFYITNKYPMNLAH
                     LESRLKNNGYLKSYQSLDKDILKQLLCERFATIDYASAKSDVSPFIRDRKVVDFWSAD
                     LFIAATKDFDF"
     gene            complement(1884..2474)
                     /locus_tag="PKJ65_02405"
     CDS             complement(1884..2474)
                     /locus_tag="PKJ65_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40723.1"
                     /translation="MIYTTNMLLEKYSNYSNAYNKIQRDCKNGKLVKLMRGLYEDNPS
                     VSPHLCSGYIYGPSYISFDYVLSQSGLIPEYVPNITCATFRKNKIKQYDTPLGSFIYR
                     DIPPEVFPLATIARIEDGYSYIIATPEKALCDKLYSIPQVSSVSAFEELMFDDLRIDK
                     DELSKLDWEIIKSIAPKYNKRNLDYLLKYHAKETEK"
     gene            complement(2667..3917)
                     /gene="rlmD"
                     /locus_tag="PKJ65_02410"
     CDS             complement(2667..3917)
                     /gene="rlmD"
                     /locus_tag="PKJ65_02410"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNZ40724.1"
                     /translation="MKQAELNIIDISYEGKGIARKDNLVYFTDNALDGETVTAEAHEI
                     KPKYVKAKTIQILKPSKYRTEPKCVVYDKCGGCNLRHMSYERQLKLKKDTVIANLKRM
                     AHINIEDITIHQNPKTDYYRNKAEYKIKNGKIGFYEDKSHNIVTHEECLLLKEEINNI
                     KKSIEKHITKDMTALTIKEADDGYMVIAESDKPLPNKLINTLKENNIVLSVYQRKQNR
                     NIQIYGKQYITDNICGLKFQISPDSFYQVNKMQTEKLYNIAKHYLKAADKDSNLKDKT
                     VLDLYCGIGTIGLFIADSVKYVIGVEIVKEAIDNAKSNAKLNTIENCQFIHADVNDAI
                     LRIEYDILIADPPRKGLDDSVIQAIRQKKPESLIDISCNSSTLARDIGQLSDLYSLSE
                     IEAVDMFSHTSHVETVTLLSRKTG"
     gene            complement(3918..>4562)
                     /locus_tag="PKJ65_02415"
     CDS             complement(3918..>4562)
                     /locus_tag="PKJ65_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HNZ40725.1"
                     /translation="DRTFPEWELYKHHPYIRSRTDQAKIALDYADENFAVSILELPYI
                     FGAQKGRKPVWTFLVDMIRKMPVVTMYPKGGTAMITARQVGQCIASALEIGKGGQLFP
                     VATYNMTWKEMLAMFHEHMGMPNRKIVTVPTCFFKASVKKLAKQQKAAGHEGGLDMVK
                     FVKVMTYEAYMSDFNAKTIFRARPDDINKAIGESVRLCLDILDSNTQVIDMKGE"
BASE COUNT         1197 a          949 c          834 g         1582 t
ORIGIN      
        1 caacgtttat gttggggcat attccgttat cttatccgcc gaaatatcat tgtggcatta
       61 ttgatttatc ttcttataca ctttcgcttt aaaatataca taactaaaaa gtttataatt
      121 ccggatttct gtccggtcaa atttagagtt tttattcggt catatattca taatgtcatg
      181 gctaatgtct ctgcaaactt tacaaaaccg actttatcag cgagtttctg tgatgctgca
      241 ttatctattt ccgtagacca tatcgggcat ttgccaagtt ttataatgct ttttgcacat
      301 ttgatacaaa cttccgatgc ataacctcta ccgcgatatt ccggaagtgt gttaactcct
      361 atttcctgaa ctgtctcctg catataaggc attcccggag catccgaaca acttacgagc
      421 ttactgtttt caaatattcc gacacaaaga ccatattgta ccatttcgtc aaagtattca
      481 cgtagccagt ctgtattatt acatcctaag tgagctttcc tgaaaaactc gagatatgag
      541 ggatagtcgg agcaggataa aataaccgca caacttaccg catttgtttc tctgtaaaca
      601 tatttgatgc tttgtgtaat cggcataccg aatgtgtccg acaacaactc cttaacctta
      661 gctgatgaca ttgctacatt catcttttgc ttaagttcat ttattttttc ggatgcttta
      721 tccccatagg aaactattag ctttcccggc tgaagccaaa cataaatgtc aaaaggatgt
      781 gaataaccgt actgtgtgat attacgttct ttagaatata tgaagtgcat acctcgagtt
      841 tcaaacgatt cagatgatat accgagccac tttgtgtaat actgtttggt tattttatga
      901 taattcatat atcttccagc ctacctctct aaaatttcaa aatataaata tagtatttac
      961 ctttttctgc cggttattaa tggaacaacg cgagcaaccg ctttcacata tccttcctgc
     1021 aaattaaaaa tcaaaatctt tagttgcagc aataaacaaa tctgccgacc aaaagtcaac
     1081 aactttcctg tcacgaataa acggagaaac atcagatttt gctgatgcat aatctattgt
     1141 cgcgaacctt tcacaaagca attgcttaag tatatctttg tcgagcgatt gataagattt
     1201 cagatagccg ttgttcttca gcctgctttc cagatgtgcg agattcatag gatatttgtt
     1261 agttatgtaa aaaagatagt catagagatc gcgccccttg actctgcttt tccaacttcg
     1321 cgaaagtacg gcagaaactt tcccggcaaa caatgtacct ttatcgtaaa gtcttacagg
     1381 ggtaggatat ggctgtagca gatatttcgt ttcatagctt gcagcggaaa gtgctgctgc
     1441 atccgcttca agttttatct tcaatacttc gttagccgga actcttcctg taaagacctc
     1501 ttcggttccg aaacaattta aaatttccct gcaattgcct ttgatatatc ctctttgtat
     1561 aaatccgctt ttcgaatcaa cttcaaatgt tgccggaata ccaagagaag taagctcatt
     1621 gttgatacag ccgacatact tatcaagtac gaatgtatct attttttcgt tatcgataga
     1681 aaaatccaaa tcttctgaaa atctttggag accgtgaaaa attctgagag ccgttccgcc
     1741 gttaaaagct gcattctgat agaagtcaga acgtcccagt ccgcataatg taatttcctg
     1801 caaggcttgc ttaactgcgt tttttgcttc cgcatcgctt gtagccacgg gatatttact
     1861 catcatggaa tctaatacac tattcatttt tcggtctcct ttgcatgata tttcaaaaga
     1921 taatccaagt tgcgtttatt gtattttggg gcgatgcttt taataatctc ccagtcaagc
     1981 ttgctcagtt catccttgtc gatacgaaga tcatcaaaca tcaattcctc aaatgcggat
     2041 acggagctaa cctgcggaat gctgtacagt ttgtcgcaga gtgccttttc gggagtggca
     2101 attatgtatg aataaccgtc ttcaatcctt gctattgttg caagcggaaa cacctcaggc
     2161 ggaatatccc tatagatgaa ggaacccaat ggagtatcat attgcttaat cttattcttc
     2221 ctgaaagtgg cgcatgtgat attcggaaca tattcgggaa taagtccgct ttgtgacaga
     2281 acatagtcaa aggaaatata tgagggcccg taaatgtacc cgctgcaaag gtgaggtgaa
     2341 acagaaggat tgtcctcgta caaaccccgc atcagcttta caagctttcc gttcttgcaa
     2401 tcacgttgta ttttattata tgcattggaa tagttgctgt acttttccaa aagcatgttt
     2461 gttgtataaa tcatattctc ctccaacaac tcccagtata gagtagttat tggagaaaat
     2521 caagagtaaa atgtataatc cgcatagtat atgaaaggat attgaagaga acacaccatt
     2581 ttcaggcact gcattggaaa ccgtattgca gaaatagaaa gggtctgcgg aaagttcaaa
     2641 cgggattccg ttttctataa taacggttat ccggttttgc gggatagtaa ggtaaccgtc
     2701 tccacatgac ttgtatgact gaacatatcc acagcttcta tttcggacag ggaatagagg
     2761 tcgcttaact ggccaatatc tcttgcaagt gtagagctgt tacaggagat gtctattaag
     2821 ctttccggtt tcttttgtct gatagcctgt ataaccgaat catccaatcc ttttctcgga
     2881 ggatcggcga taagtatgtc atattcaatc cgcaagattg catcatttac atcggcgtga
     2941 ataaattgac agttttctat ggtattaagt tttgcattgc ttttagcgtt atctatcgct
     3001 tctttcacta tttccacacc tatcacatac tttacgctgt ccgcaataaa aagacctatt
     3061 gtacctatac cgcagtacag gtctaaaact gttttatctt ttaagttact gtctttatct
     3121 gctgctttca gatagtgctt ggctatgtta tacagttttt cggtctgcat tttgtttacc
     3181 tgatagaaac tgtccggtga tatttgaaat ttcagaccgc atatgttgtc ggttatgtat
     3241 tgttttccgt agatttgtat atttcggttt tgtttccttt gatataccga taggactata
     3301 ttgttttctt tcagagtatt aatcagtttg tttggtagag gtttatcgct ttccgctata
     3361 accatatatc cgtcatcagc ctcttttatt gttaacgcag tcatgtcttt tgtaatatgt
     3421 ttctctatgc ttttctttat attgttaatc tcttctttaa gaagcaggca ctcctcatgc
     3481 gtaacgatat tatggctttt atcctcataa aaccctatct ttccgttctt aatcttatat
     3541 tccgccttat tgcgataata atccgtttta gggttttggt gtattgttat gtcttctata
     3601 ttgatgtgtg ccattctttt taagttggct ataactgtat ctttcttaag tttaagctgt
     3661 ctttcataag acatatgcct taagttgcaa cctccgcact tatcgtatac aacgcatttc
     3721 ggttcggttc tgtattttga cggtttcagt atttgtattg tctttgcttt tacatatttc
     3781 ggtttgattt catgcgcttc tgctgttacc gtttctccgt caagagcgtt atctgtaaaa
     3841 tagacaaggt tatctttcct tgctatccct tttccttcgt atgatatatc aattatgttc
     3901 aactctgctt gcttcaacta ttcgcccttc atgtcgatga cttgggtatt gctgtccaat
     3961 atgtctaagc ataaccgtac cgattcgcct atagccttgt ttatatcatc cggccgtgcc
     4021 ctgaatatgg tctttgcatt gaaatcgctc atgtaagctt cataggtcat tacttttacg
     4081 aatttaacca tatccaatcc gccctcatgt cctgcggctt tttgctgctt tgccagtttc
     4141 tttacgcttg ctttgaaaaa gcaggtgggt actgttacta tctttctgtt gggcattccc
     4201 atatgttcat ggaacattgc aagcatttcc ttccatgtca tgttataggt tgcgacgggg
     4261 aagagctgac cgccttttcc tatttccaga gcggaagcta tgcattgacc tacctgtctt
     4321 gcggtaatca tagcggtacc gcctttggga tacatggtca ctaccggcat ttttcttatc
     4381 atatccacta aaaatgtcca gaccggtttt ctgcctttct gggcaccgaa tatatagggc
     4441 agttccagaa tggatacggc aaagttttca tcggcataat ccagagctat ttttgcctga
     4501 tccgttctgc tccttatata cggatggtgc ttgtacagtt cccattcggg gaatgtgcgg
     4561 tc
//
LOCUS       DAOPWC010000071         4519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_19293_length_4519_cov_3.009409, whole genome shotgun sequence.
ACCESSION   DAOPWC010000071 DAOPWC010000000
VERSION     DAOPWC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19293_length_4519_cov_3.009409"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PKJ65_02420"
     CDS             <1..1014
                     /locus_tag="PKJ65_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ40726.1"
                     /translation="ILLCVIMVLSFAGCAKDREVLNVYNWGEYIDMEVLDMFEEETGI
                     KVNYNTFVTNEDMYVKLSKGRVSYDVIFPSDYMIQRMIEEDMLQKINMDNVPNYSNIA
                     DVHKNLSFDPNNEYSVPYLWGTVGILYNKTMVSDTVDSWDILWNEKYSQKIFMQDSQR
                     DSMMVALIRLGYSQNTTDQKQVDQAKDLLIAQKPLVLGYLVDEVKDKMIQGEGALAVV
                     WSGEAVDAMSMNKDLAYVIPKEGSNRWVDSMVIPKNAKNVSAAEKFINFMLRDDIMVM
                     NANETGYSITSKTAIELLDEDWRNNLAAFPTEDMLSNCEVFEYNAQATVMYNKAWTEV
                     TAN"
     gene            1085..2107
                     /gene="mreB"
                     /locus_tag="PKJ65_02425"
     CDS             1085..2107
                     /gene="mreB"
                     /locus_tag="PKJ65_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNZ40727.1"
                     /translation="MGLDIGIDLGTSSVLIYVKGKGVVLREPSVVSRDEDGKVKAVGE
                     DACLMIGRTPANIEAIRPLRDGVISDYEATEEMLKYFIKKVCGKKLLLYKPRIMICVP
                     SGITEVERRAVMDAADKVGAKEVYIIEEPIAAAIGAGLDITKAYGSMVVDVGGGTTDI
                     AVISLCGIVKSKSIKIAGDTFDEAIIRFMRRKYSLQIGERMAEEMKIRIGCVYPRKEE
                     VSMQVRGRNLVLGLPRTIEITSSELMEAFDEVVQQIVDGVKNVLEKTPPELSADIGDR
                     GIVLTGGGSLIYGLDTLLSIKTGLEVTISDDPISCVVLGTGEALENLDKILKLRVIKQ
                     EERSHR"
     gene            2109..3359
                     /locus_tag="PKJ65_02430"
     CDS             2109..3359
                     /locus_tag="PKJ65_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNZ40728.1"
                     /translation="MKTKGLRLYGVNDLRLDEFELPRIKEDEILARVITDSICMSSYK
                     AAIQGAGHKRVPKDIAKNPVIIGHEMCGEILEVGKKYKGRYEKGQLFTMQTSLNHPSN
                     IYAAPGYSFRYTGGTIQNMIVAKEVMEMDCLLEYNGDSYYQGSLAEPYSCIAGAFAAM
                     YHTNRVNYEHTMGIKEGGKMAILAGAGPMGLGAIDYAVNQKKKPSLLVVTDIDDARLA
                     RAAEIFSPEWAAEHGIKLVYLNTGKAEDPVRALMELSDNKGYDDVFVFAPVKPVVEQG
                     DRILGYDGCLNFFAGPSDTTFSASFNFYNVHYNSTHVVGTSGGNTEDMKECITMFEEG
                     KLNPAAMVTHIGGLDSAAETTLNLPDIPGGKKLIYTGISMPLTAISEFKEKGYPELAN
                     ICDANKGLWCKQAEDWLLKHSKKI"
     gene            3521..3988
                     /locus_tag="PKJ65_02435"
     CDS             3521..3988
                     /locus_tag="PKJ65_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40729.1"
                     /translation="MNNTDNARINMAIYIISLIAGTVIYWLILSSYITSAFSFALSAA
                     GYAISILFLLTRYNEYIINKDYRVMNKYCDDVMSVCPKCGHMHITAYSRVFNSRIFMN
                     ILKYIAFQKDARPVYIIRIAKCNKCGHIWYGGYRNVDKINSSFDENCVQNKVS"
     gene            4030..>4519
                     /locus_tag="PKJ65_02440"
     CDS             4030..>4519
                     /locus_tag="PKJ65_02440"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ40730.1"
                     /translation="MKNKMNKTEWSWVLYDVANSAFTMLTTALVPIYFAAMVANTSTI
                     ESNEATALWGSANSVAILILALLSPVLGAIADYKGMKKKMFAAALSVGLLGAFAMAFM
                     NTWLSFLIIYVVARIGYSATNVFYDSMLTDVTTDDRMDRVSSNGYAYGYIGSCIPFIL
                     CLV"
BASE COUNT         1465 a          751 c         1081 g         1222 t
ORIGIN      
        1 attctgttat gtgtgataat ggtgttatct tttgcaggat gcgcaaagga tagagaagta
       61 ttgaatgttt acaactgggg cgaatacatc gacatggaag ttttagacat gtttgaggaa
      121 gaaaccggta ttaaggttaa ctacaataca ttcgtaacaa atgaggatat gtatgtaaag
      181 ctttccaagg gcagggtgtc atatgacgtt atcttcccgt ccgattatat gatacaaaga
      241 atgattgaag aggatatgct ccaaaaaatt aatatggaca atgtacctaa ctattcgaat
      301 atagccgatg tacacaagaa tctttcgttt gatccgaata acgaatactc cgttccctat
      361 ctttggggaa ctgtcggaat attgtacaac aaaaccatgg taagcgatac tgtagacagt
      421 tgggatatac tgtggaatga aaaatattcg caaaaaatat ttatgcagga cagtcaaagg
      481 gactccatga tggtagccct tataagattg ggttactcgc agaacactac cgaccagaag
      541 caggtagatc aggcaaagga tcttttaatt gcacaaaagc cgttggtatt gggctatctt
      601 gtagacgaag taaaagataa gatgatacaa ggcgaaggtg cgttggcggt ggtatggtcg
      661 ggtgaagcgg tggatgccat gagtatgaat aaagaccttg catatgttat accaaaagaa
      721 ggaagtaaca gatgggtgga cagtatggta ataccaaaga atgcaaagaa tgtatcggca
      781 gcggaaaaat tcataaactt catgttaaga gatgatataa tggtcatgaa cgcaaatgaa
      841 accggttatt ccattacttc aaaaaccgca attgaactgc ttgatgagga ctggagaaat
      901 aatctagctg cattcccgac tgaagatatg ctgtcaaatt gcgaagtttt tgagtataat
      961 gcacaagcta ctgttatgta caacaaagca tggacggaag ttaccgcaaa ttaaataaat
     1021 atagtttttt attatatgat ttatagtata taattataat aaataatatg taaggagcaa
     1081 tggtttggga ctggatattg gaatagatct cggtacttct tcggtactta tatatgttaa
     1141 aggaaaaggt gtggttttac gtgaaccttc cgtcgtatca agagatgaag acggaaaagt
     1201 caaggctgta ggtgaggatg catgccttat gataggcaga actccagcca atattgaggc
     1261 gataagaccg cttcgtgacg gtgtcatatc cgattatgaa gccacggagg aaatgctcaa
     1321 gtactttatt aagaaggttt gcggcaagaa gcttctgctc tacaaaccgc gcataatgat
     1381 atgtgtaccc agcgggataa ccgaggttga aagaagagcg gttatggatg cggccgacaa
     1441 agtcggtgca aaggaagtgt atattatcga agaacccatt gcagccgcaa taggagccgg
     1501 actggatatt accaaggcat acggaagtat ggtggtggat gtcggcggcg gaacaacgga
     1561 tattgcggtt atttctcttt gcggaattgt taaaagcaaa tccataaaaa tagcggggga
     1621 tacatttgat gaagccatta tcaggtttat gagaagaaaa tacagccttc agataggtga
     1681 gagaatggcc gaggaaatga aaatacgcat cggttgtgta tatcccagaa aagaagaggt
     1741 ctcaatgcag gtcagaggca ggaatctggt tttgggattg cccagaacca tcgaaataac
     1801 ttcttcggaa cttatggagg cctttgatga ggtggtccag caaattgttg acggagttaa
     1861 gaatgtgctg gaaaagacgc ctccggaatt atctgcggac ataggtgaca gaggtatagt
     1921 attgaccggt ggcggcagcc ttatatacgg actggatacc ttactttcca ttaagaccgg
     1981 tctggaggtt accatttccg acgatcccat atcctgcgtt gttttaggta ccggagaagc
     2041 attggagaat cttgataaaa tcttgaagct cagggttatt aaacaggaag aaaggagcca
     2101 cagatagtat gaagacaaaa ggattaagat tatacggagt gaatgatctt cgtttggacg
     2161 aatttgaact gccccgaata aaagaggatg agatattggc aagagttata accgacagta
     2221 tatgtatgtc atcatataaa gccgccatac agggagcggg acataaaaga gtaccgaaag
     2281 atatcgcaaa gaaccccgta ataatagggc atgagatgtg cggagagata ctggaagtgg
     2341 gcaagaaata taaaggcaga tatgaaaagg gacagctttt tacaatgcag accagcctta
     2401 atcatccttc caatatatat gcggctccgg gttattcatt cagatacacc ggcggaacga
     2461 tacagaatat gatagtagcc aaagaggtta tggaaatgga ttgcctgctg gagtataacg
     2521 gagattccta ttaccaaggt tcattggcgg aaccttactc ctgcatagcc ggtgcttttg
     2581 ccgcaatgta tcacacaaac agagtcaatt atgagcacac aatgggcata aaagaaggcg
     2641 ggaaaatggc aatactggca ggagccggac cgatgggttt gggtgcaata gattatgccg
     2701 ttaatcagaa aaagaaacct tcacttttgg ttgttaccga tatagatgat gccagactgg
     2761 caagagcggc cgagatattt tctcccgaat gggccgccga acacggtatc aaacttgtat
     2821 atctcaatac cggaaaagca gaagatcccg ttagagcact tatggaactt tccgataata
     2881 aaggatatga cgatgtattc gtgtttgctc ccgtaaaacc cgtagtggag cagggggaca
     2941 ggattttagg ctatgacgga tgtctaaatt tctttgcagg accgtcggat accacttttt
     3001 cggcatcttt caacttctat aatgtgcatt ataactctac tcatgtagtg ggaacaagtg
     3061 gcggaaatac tgaagatatg aaggaatgta taaccatgtt tgaagagggt aagctcaatc
     3121 ctgcagctat ggttacacat ataggcggat tggattccgc tgcggaaacc acattaaatc
     3181 ttcccgatat accgggagga aaaaaactta tctataccgg aataagtatg cctcttaccg
     3241 ctatcagcga atttaaagaa aaaggatatc ctgaactggc aaatatttgt gatgcaaata
     3301 agggattgtg gtgtaagcag gcggaagact ggcttttgaa gcacagtaag aagatttagt
     3361 ttaaatttgt aatcaatctt taatagtaaa taattcagat aaaacagctt tttgacaagc
     3421 tgttttgcta tatattaagc taaaaaatgc gatataagtc cgtattatag tacatatctg
     3481 ttgttattat gtatatatcc cgggaaaggc ggtacatgat atgaacaata cggataatgc
     3541 aagaataaat atggctatat atattatctc attgatagca ggcacagtta tatactggct
     3601 tatattgtct tcctatataa cttcggcttt ttcctttgca ctgtcggcag cgggttatgc
     3661 aatcagcatt ttgtttttgc tgacgaggta caacgagtac attatcaata aagattaccg
     3721 ggttatgaat aaatactgtg atgatgttat gtcggtatgt cctaaatgcg ggcacatgca
     3781 tataaccgct tacagcagag tatttaacag cagaatattc atgaatattc tgaagtatat
     3841 cgcatttcaa aaggatgccc ggcctgttta tatcatcaga attgccaaat gtaataaatg
     3901 cggacacata tggtatggcg gttataggaa tgttgacaaa ataaattctt catttgatga
     3961 aaattgcgtg caaaataaag tcagttagtg taaaatagca tcaacaggta caaacggggg
     4021 aatttaataa tgaagaataa aatgaacaag accgaatgga gctgggtgct gtacgatgtg
     4081 gctaattcag catttacaat gctgacaaca gctcttgtgc ccatttattt tgccgcaatg
     4141 gtagccaata caagtaccat tgaatccaat gaggcaacag cactatgggg aagtgcaaat
     4201 tcagtggcaa ttttaatact tgcattgctt tcacctgttt taggtgcaat agcggactac
     4261 aagggtatga agaagaaaat gttcgcagca gcattgtcgg taggtctgtt gggagctttc
     4321 gcaatggcat ttatgaatac atggctttcc ttcctgataa tatatgttgt tgcaagaata
     4381 ggttattcgg caaccaatgt gttctatgac tccatgctta ccgatgtgac aaccgatgac
     4441 agaatggaca gagtctcatc caacggttat gcatacggat atataggcag ttgtattcct
     4501 tttatattat gtcttgtgg
//
LOCUS       DAOPWC010000072         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_19390_length_4501_cov_2.945794, whole genome shotgun sequence.
ACCESSION   DAOPWC010000072 DAOPWC010000000
VERSION     DAOPWC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19390_length_4501_cov_2.945794"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..133
                     /locus_tag="PKJ65_02445"
     tRNA            58..133
                     /locus_tag="PKJ65_02445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91..93,aa:Phe,seq:gaa)
     gene            139..213
                     /locus_tag="PKJ65_02450"
     tRNA            139..213
                     /locus_tag="PKJ65_02450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Gly,seq:gcc)
     gene            223..297
                     /locus_tag="PKJ65_02455"
     tRNA            223..297
                     /locus_tag="PKJ65_02455"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:255..257,aa:Cys,seq:gca)
     gene            complement(389..1153)
                     /locus_tag="PKJ65_02460"
     CDS             complement(389..1153)
                     /locus_tag="PKJ65_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40731.1"
                     /translation="MIRGIESFRKWFQGCEEQYVIIGGTACDLLMTNEGLDFRATKDI
                     DLVLVIEALDVSFGKRFWEYIVAAGYEHRNKSTEAYQFYRFSKPQLQEYPAMIELFTR
                     KPDTIILPRNAILTPLPLDENISSLSAILLDDDYYNFLMQGRTKVAGVTVLDATHLIA
                     FKAKAWLELSERKVACGDVDSRDIKKHKNDVFRLTELLYPDQEPLRNVPLTVLKDMRT
                     FLEKMETEDVDLIKLRIPNKTKDAILEELRSLYGLD"
     gene            complement(1137..2096)
                     /locus_tag="PKJ65_02465"
     CDS             complement(1137..2096)
                     /locus_tag="PKJ65_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ40732.1"
                     /translation="MGYLNTLLGIHVVYKGDIVRNIPNFIHARYKLQKATLDGNEVIF
                     LFPKTELEPVSAIKKHIERIQKEENLHVILVPKKLTYRQKEYLLRDRIPFIVEGKQIY
                     LPFMGTYLQERSDGEKQMTDVMLPSAQLLFLYYIYNGCGELLTSEAAKALELTPTSIS
                     RASIQLEGMGLIKTEKRGVQKVVFSNKTVQELFTEGQKHLANPVKRRVYVPKSEIKER
                     LLFSGYSALSNYSMLNPSDIDFYATDSIARWEKIASKKLLNEDEQCAVEFWSYNPRKL
                     SKDENVDRLSLALSLREDKDERVEEAVQEMLEQVWRDIDDKRN"
     gene            complement(2419..3279)
                     /locus_tag="PKJ65_02470"
     CDS             complement(2419..3279)
                     /locus_tag="PKJ65_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40733.1"
                     /translation="MKKTKLLYIVCCIVFISILVACQINKEKQPEASDNTEIRSVEDT
                     QTVVFVDYNWVVLKKEDAYTKNTVILTGEVSNVRYTTVDYEFMDTNVSDKATLFDIKV
                     DKVLSCTSDLYPDRDCITVGVGYTMSTYNEELPVITEGARYLLFCYVTADDKDDVRET
                     YKYADCWICYPKDLLCENIDGYYLVNDFFSDIPSAVSLAGAIGVTKKDASDIFSHVSK
                     EYIKRKMEASSKRKVISEEVALSCIETLKVRAGNGADQLWNIMKNRYLIEETAFENHI
                     RKTAESFSDR"
     gene            complement(3306..>4501)
                     /locus_tag="PKJ65_02475"
     CDS             complement(3306..>4501)
                     /locus_tag="PKJ65_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNZ40734.1"
                     /translation="FRKGDVIINIPENPTGQVTESSDLSSPTYTRQYTPIEGLNRYLP
                     RKNNPQNVTLTADKIKVINDMFNSIVDNDATVYISFPDSSIACYENDKFNLNTGIFHE
                     KHNYIATVTIVSDSIIKSGYLLADRDGTIYMDEIKKSTMKHDVSLRIINRDNPITQAE
                     ANDTTGLIQLKDKNYVLDNRDDLFILPHVKQALEELLAAADKQDASLKMHVASAYRTY
                     DSQKQSFDYWVNRRVTQENMTYAQAYEYTAGRVAIPGCSEHHNGLTLDVVGYGYMMDE
                     SSKGAPYAIWLKNNSYKYGFTVRYEEGKEQYTKITLYEPWHLRYVGLLTAFYLYNENL
                     CMEEFYQKLLTEKLIDFTYDNETYRYIYAKNDNIYIDENVMETVQYSRIASNVEGYVV
                     LCKVK"
BASE COUNT         1226 a          993 c          775 g         1507 t
ORIGIN      
        1 aatgccagcc tgtcacgctg gaggtcgtgg gttcgagccc catccaggtc gccatatgcc
       61 cagatagctc agtcggtaga gcaggagact gaaaatctcc gtgtcggtgg ttcgattccg
      121 cctctgggca ccaaacgagc gggtttaact cagtggtaga gtgtcacctt gccaaggtga
      181 aagtcgcgag ttcgaatctc gtaacccgct ccatttattt atggcgccat aaccaagcgg
      241 taaggtagag gtctgcaaaa ccttcattct ccggttcaaa tccggatggc gcctccagat
      301 tacggtagac ggacacaaaa gtgtgttcgt cttttttatt tacatcaata gctaatatat
      361 ctttcagcat tacttccctg gcagttgatt aatctaaacc gtacaaagaa cgcaattctt
      421 ccaatatagc gtctttggtc ttattcggta tacgaagctt tatcaaatcc acatcttccg
      481 tttccatttt ctctaaaaag gttctcatat ctttcagcac tgtaagcggc acattccgaa
      541 gcggctcttg atcagggtaa agcagctccg ttaagcgaaa tacatcattc ttatgtttct
      601 ttatatccct actgtctaca tcaccgcagg ctactttcct ttcagacaac tccaaccatg
      661 ccttagcttt aaaagcaatc agatgagttg cgtctaaaac agttactcct gcaacctttg
      721 ttcttccctg cattaaaaaa ttatagtaat catcatcaag aagaatagcc gatagactgg
      781 aaatattttc atctaacggg agcggtgtaa gaatagcatt tcgaggcaga atgattgtgt
      841 ccggttttct tgtaaacagt tcaatcattg cagggtattc ttgcaattgc ggctttgaaa
      901 agcggtaaaa ttgatatgct tccgtgcttt tgttgcgatg ttcataccct gcagcaacta
      961 tgtattccca aaatcttttt ccgaacgaaa catctaatgc ttctataacc aatacaagat
     1021 caatatcttt tgtcgcacga aaatccaacc cttcatttgt cattaacagg tcacaagcag
     1081 tcccccctat aatcacatat tgctcttcac agccttggaa ccattttcta aagctttcaa
     1141 ttcctcttat catcaatatc cctccatacc tgctcaagca tttcttgcac agcttcctct
     1201 accctttcgt ccttatcttc ccgaagagat aatgcaagag aaagcctgtc aacattttcg
     1261 tccttggata gttttctcgg attgtaactc caaaattcca ctgcacattg ctcatcctcg
     1321 tttagcaact tctttgaagc aatcttctcc catcttgcaa tactgtctgt agcataaaag
     1381 tcaatgtcag acggattcaa catagaataa ttggaaagag cagagtaccc gctaaaaagc
     1441 aatctttctt ttatctcact tttcggtaca tatactcttc tctttaccgg attagccagg
     1501 tgtttttgcc cttcagtaaa gagctcttgc acagttttat tggaaaaaac aactttctgt
     1561 acacctcttt tttccgtctt aattaacccc ataccctcaa gttgtattga tgctctggag
     1621 atagatgtcg gtgttaattc caatgctttg gcagcttcac tggtcagtag ctcaccgcaa
     1681 ccgttataaa tgtagtatag aaacagcagt tgtgcggaag gaagcattac atccgtcatt
     1741 tgcttttctc catcgcttcg ctcctgaaga taagttccca taaaaggaag gtatatctgt
     1801 tttccctcca caataaacgg tatacgatcg cgcagaagat attctttctg cctatatgtc
     1861 agtttcttag gaacaagaat aacatgcaaa ttttcttctt tctgtatcct ctctatatgt
     1921 tttttaatag cgcttacagg ttccaattca gtcttcggaa ataggaatat aacttcattt
     1981 ccgtcaagag tggctttctg cagcttatat cgtgcatgaa taaaattggg tatattcctc
     2041 acaatatcgc ccttatatac tacatgtatt ccaagtaaag tattcaaata tcccatcact
     2101 tccgcccttt cttaactctg atttgtatta taactcaatt taagttataa tgcaataact
     2161 aagttaagat atggattaat aatcaactaa aacctaatta gctacacctt tttagttttt
     2221 aagtgaccct gtgttagtat caggctacca caggaacatt aacctcattt ttggaaattt
     2281 gctatatcat tatgatttgc aggtaaaaac caataatctg catgcaatca ggcaataatg
     2341 gtgcagaata cttgcatatc ctgcaccgaa tattacacta cattcggtgc agtattaatt
     2401 tgttataacg gtttctgttt atcggtcgga aaaactttcc gctgtcttcc gaatatgatt
     2461 ctcaaatgcg gtctcttcta taaggtaacg gtttttcatt atattccata attgatccgc
     2521 tccgtttccg gctcggactt tcaatgtctc tatgcaggaa agtgccacct cttcacttat
     2581 tactttcctc ttcgatgagg cttccatttt tcttttaata tactcctttg atacatgtga
     2641 aaagatatca gatgcatctt tttttgtcac tcctattgca cctgcaaggc ttacggcaga
     2701 cggaatatcc gagaaaaaat cgtttacaag ataatatccg tcaatgttct cgcacagcaa
     2761 atccttggga taacaaatcc aacaatcggc atatttgtat gtctctctca cgtcatcttt
     2821 gtcgtcagcc gtgacatagc agaacaaaag atagcgtgct ccttccgtta ttacgggaag
     2881 ttcttcgttg tatgtactca tagtgtaacc tacacctact gtaatgcaat cccggtcggg
     2941 atacaaatcc gatgtacagg ataaaacttt gtctacctta atatcgaaca gtgtggcttt
     3001 gtcggaaaca ttcgtatcca tgaattcata gtctacggta gtatatcgca catttgaaac
     3061 ttcaccggtc aatattaccg tgttttttgt ataagcatct tctttcttaa gaacaaccca
     3121 gttataatca acaaagacaa ctgtttgcgt atcttcaacg gaccggatct ctgtattgtc
     3181 cgatgcttcc ggttgttttt ctttgttaat ctgacaggca actaaaattg agataaatac
     3241 tatacagcaa acaatgtata ataactttgt tttcttcatt atggtacctc ccgattttta
     3301 tgcttttatt tcaccttaca cagtaccaca taaccctcaa catttgaagc tatgcgcgaa
     3361 tactgtactg tttccattac attttcatct atgtatatgt tatcattttt tgcgtatata
     3421 tatctgtatg tttcattatc gtatgtaaaa tctatgagct tttcggtcaa cagtttttga
     3481 taaaactcct ccatgcataa gttctcattg taaaggtaaa atgcggtgag taatcctaca
     3541 taccgtaagt gccagggctc atatagagta atctttgtat actgctcctt gccttcttca
     3601 tatcttacgg taaatccgta tttataactg ttgtttttaa gccagattgc atatggagca
     3661 ccttttgacg attcatccat catgtagccg taacctacca catccaatgt cagtccgtta
     3721 tgatgctcac tgcagccggg tattgctaca cgtcccgctg tgtattcata ggcctgtgca
     3781 tatgtcatat tttcctgagt cacccttctg ttgacccagt aatcaaaact ctgtttttga
     3841 gagtcatatg ttctatatgc acttgcaaca tgcattttaa gacttgcatc ttgcttatcg
     3901 gcagctgcta atagttcttc caatgcctgt ttaacatgag gcagaatgaa aaggtcatct
     3961 ctgttgtcta aaacatagtt tttatcctta agctgtataa gtcccgttgt atcgtttgct
     4021 tcggcttgcg ttatagggtt atccctgttt atgattctca gggaaacatc atgtttcatt
     4081 gtactctttt ttatttcatc catatagatt gtaccgtctc tgtcggccaa aagataaccc
     4141 gactttatta tgctgtccga aacaatcgta accgtggcta tatagttatg tttttcatga
     4201 aatattccgg tattcagatt gaatttgtca ttctcataac acgcaatgga gctatcgggg
     4261 aaagatatat aaactgtggc atcattatca acgatactgt taaacatatc atttattacc
     4321 ttaatcttat cagctgtaag tgttacgttt tggggattgt ttttacgggg aagatatcgg
     4381 tttaaccctt caatcggagt atactgccgt gtatatgtag gggaagataa gtcggaggat
     4441 tccgttacct gccctgtcgg gttttcgggt atatttatta taacatcccc tttacggaaa
     4501 a
//
LOCUS       DAOPWC010000073         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_19398_length_4499_cov_4.009226, whole genome shotgun sequence.
ACCESSION   DAOPWC010000073 DAOPWC010000000
VERSION     DAOPWC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19398_length_4499_cov_4.009226"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /locus_tag="PKJ65_02480"
     CDS             complement(<1..2355)
                     /locus_tag="PKJ65_02480"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40735.1"
                     /translation="MKVKRKLLSVFLVISMLAGILPLTALPVYAANPPATDVSVYTTE
                     GNQTLDSMYPYLVNGKKAKTGTLGSGGCTAYFDHTTGILTLQNHSGGNIVKGSLGGKK
                     DLTVKLVGTNALTSALQNISGGDLIITAEDTATLSITNNTDTNGVITGISTDITGVNA
                     GPGSITIRGKANISIYVENISTGVESGVRGIFAKQDISILDNASVNIECIGKGTGSYP
                     VSGIQSTNKSVTFNTTGKVNIDCSDAQNKNPYAINNMLANILTSVDELTLKWTNGGRG
                     YSLVDGGMSYDVTSFTRNIKEATPTQPGIEVFKKGTPLTLTIENGKNNFGKKEGQFTH
                     GETVNVTANSITGLTFKKWVATAGIIGCATDSSTTLTMPDENVTLTAIYDVFAVQPKS
                     AYVSATSRKVTFSLKATPTTYAPRLVTESGDYNDIVGETTFGHDGALNYSEVIRCIGI
                     FDVPNGNYRIAAKFDDVWYYSDVFTVDYSTHSITVDGDAVETTDYTITGVSDFSSIPD
                     GTNLTINAVNDINVDGENVVAGSSKTIVINSSYTITINKKVSNSAPTVVVGKETQTGN
                     ATPKASSGIPAAIAYEADMSGWFNDADGDALTYSVVSAREGTNDVSAHVSVAGKHITY
                     TPAAAQAGKNVIIKIKANDGLADSTDNVTITVTVEEVPADAKIYKVTVAGDAAETTDY
                     TISGATDLNAVPDGTNLTIYAVNNITVNGEIVTCGNSKNITINSAQTITINKKVGNSA
                     PTVVAGKEAQTGNATPEANCGHPEAVMYEADMSGWFNDVDGDVLT"
     gene            complement(2396..2782)
                     /locus_tag="PKJ65_02485"
     CDS             complement(2396..2782)
                     /locus_tag="PKJ65_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40736.1"
                     /translation="MRQIILSMKDYIFADAVAQALLNEKGSDFAVQKTKSPEEIQKYC
                     KITSPYALLMEVTGYPPFALSERLALREQVKKQNPNCKIVILVDENSEKEIAAQVRQA
                     KKDGLIDQFIYGSTSASYLVAMMDTL"
     gene            complement(2942..3565)
                     /locus_tag="PKJ65_02490"
     CDS             complement(2942..3565)
                     /locus_tag="PKJ65_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ40737.1"
                     /translation="MVNVLIVEDKIMPRDCMEGYIKSSDRYSLVASITNAGMAEITCM
                     CYPVDLILMDVCTENNESGIKACSVIKRYFPDIKVIIVTSMADSSFITKAREANADSF
                     WYKEVSENELLDIMDRTMSGESVWPGKVPEVQIGKASSCEFTQREMEVLRLIVRGLTD
                     AAIAEELYISITTVRTHIRELLKKSGYSNRVRLACDVINKDLIVEGF"
     gene            complement(3569..>4499)
                     /locus_tag="PKJ65_02495"
     CDS             complement(3569..>4499)
                     /locus_tag="PKJ65_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40738.1"
                     /translation="FADSKGMIVLCNRQMHRLCCILLGTGLQHIFELREALQTPTDTV
                     THVEGTNDTFRFPDGRIWEFRESTVTVVGGKTYTQIQALSLTELYAKKTELEQKNREL
                     EEVNKRARKLYAELDNTVREEEAFAIKMRIHSDIGLRLLATHKVLREGGLTELKEAGK
                     IWRSTLNVFGIADAVASDYTNIEKKTSSRSEEMLHNLINSAAGIGLKIILDGNLPEDR
                     EQSYLITTAMRECATNTVRHAGGNEMKVRIFEVKSTLVVWIENNGKPPERKVVEGGGL
                     SDLRKRIEKAGGTMRIKSSPTYRLTVILPGKEK"
BASE COUNT         1129 a         1071 c          842 g         1457 t
ORIGIN      
        1 agtcaataca tcaccgtcaa catcattaaa ccatcccgac atatccgcct catacattac
       61 tgcttcagga tgaccgcagt tagcctcagg tgttgcattt ccggtttgtg cttcctttcc
      121 tgcaactacc gtaggtgcac tattgcccac ctttttgtta atggttattg tttgagctga
      181 attgatagtt atatttttgg agtttccgca agtcacaatt tcaccgttaa cggtaatgtt
      241 attaaccgca tagattgtca gattcgttcc gtccggcacc gcattcaagt cggttgctcc
      301 cgatattgtg tagtcggtag tctcggcagc atctccggca acagttacct tatatatctt
      361 cgcatctgca ggcacttcct ctacagttac ggttattgtt acattatccg tactgtctgc
      421 aagtccgtca tttgctttta tctttataat tacattttta cccgcttgtg cggcagcagg
      481 tgtataggtg atgtgctttc ccgcaacact cacatgagcg gaaacatcat ttgtgccttc
      541 ccttgccgat actaccgagt aagtcaaagc gtcaccgtca gcatcattaa accatcccga
      601 catatccgcc tcatacgcaa tcgctgcagg aataccgcta cttgccttcg gagttgcatt
      661 tccggtttgc gtttcctttc ctacaactac cgtaggtgca ctattgctca cctttttatt
      721 aatggttatg gtataggacg aattgataac tatagtcttt gagctgccgg cgactacatt
      781 ctctccgtca acattaatat cattaactgc attgattgtc aggtttgtac cgtccggtat
      841 agaactgaag tcacttacac ccgttattgt gtagtcggta gtctcaaccg catctccgtc
      901 aacggttatg ctatgggtac tataatctac cgtgaaaaca tcgctataat accatacatc
      961 gtcaaatttg gcggcaatac ggtaattgcc gttcggaaca tcgaatattc caatacaacg
     1021 aataacttct gaatagttta atgcgccatc atgaccgaaa gttgtctccc cgactatgtc
     1081 gttataatct ccggattcgg taactaaacg gggagcataa gtagtaggag ttgcctttaa
     1141 agaaaaagtg acttttctcg atgtagcgct tacataggcg gatttaggtt gtacggcaaa
     1201 aacatcatat attgccgtaa gcgttacatt ctcatcgggc atggttaaag tggtggaaga
     1261 atctgtggca catccgatta taccggctgt ggctacccac tttttaaatg tcagaccggt
     1321 aatggaattg gccgtaacat ttactgtttc tccgtgggta aattgccctt cctttttgcc
     1381 gaaattgttt ttgccgtttt ctatcgttaa agtaagaggt gttcctttct tgaagacctc
     1441 tattccgggc tgggttggtg tagcttcttt gatgttgcgg gtaaaagatg tgacatcata
     1501 tgacatacca ccatcaacta aactatatcc acgacctccg ttcgtccatt ttagagtaag
     1561 ttcatcgaca gaggttaaaa tatttgctag catattgtta atagcatatg gatttttgtt
     1621 ctgtgcatcc gaacagtcga tatttacttt tcccgttgtg ttgaatgtta cacttttatt
     1681 tgtactttga attcctgata ccgggtagga acccgtccct ttgcctatac attcaatatt
     1741 taccgatgca ttatcaagga tagatatatc ctgtttagca aagatacctc ggaccccgga
     1801 ttctacacct gtacttatgt tttctacgta tatggatata tttgcttttc ctctaattgt
     1861 tatacttccg ggtccggcgt taactcccgt aatatcggta cttatgcctg ttataacacc
     1921 gttagtgtct gtgttgttag taatcgaaag agtggcggta tcctctgccg tgattatgag
     1981 gtccccgccc gatatgtttt gcaaagcgga tgtaagagca ttagtaccga ccaacttaac
     2041 ggtcaaatcc tttttacccc ctaaagaacc tttcactata ttacctccgc tatgattctg
     2101 cagtgtcaag ataccggtag tatggtcaaa ataggcggta catccgccac ttcccaaagt
     2161 acccgttttt gccttcttcc cgttaaccag gtacggatac atggagtcga gagtttgatt
     2221 accttccgta gtatatactg atacatctgt cgccggcgga ttggccgcat ataccggcag
     2281 agcggttaag ggcagaatcc ctgccaacat ggatattacc aaaaaaacag ataataattt
     2341 cctttttact ttcatttcgc tttctccttc atatatgtat tttccttttt gttgtctaca
     2401 aggtgtccat catcgccaca aggtagcttg cacttgtgga accgtagata aactggtcta
     2461 taagaccgtc cttttttgcc tgtcttacct gcgctgcaat ctctttctcc gagttctcgt
     2521 ccacaagaat aacaatcttg cagttcggat tttgtttctt tacttgctcc cgtaaagcaa
     2581 gcctttccga cagtgcaaaa ggcggatatc ccgtcacttc catgagcaga gcatatggag
     2641 aagtaatctt gcaatacttt tgtatttctt ccggactttt tgttttttgc acggcaaaat
     2701 cactgccctt ttcattcagc aatgcctgtg cgacagcatc tgcaaagata tagtctttca
     2761 tgctcaaaat aatttgacgc accctgccac cttccttttc ttaagtaggt gatatacagc
     2821 accttgtcac tttaaattat agggaaaagg gcataaaaaa catatcgtca taaatgatga
     2881 tttttgcaac aaaagaagcc gtagtacgga aaaacggtgc ggaatagata taatatattt
     2941 tctaaaatcc ttcgacaatc aaatctttgt ttatcacatc acaggcaagc cttacacggt
     3001 tgctatagcc tgattttttg agcaactccc ttatatgtgt acgtactgtg gttatactta
     3061 tatacaactc ctccgcaata gcggcatcgg tcagacctct tacaatcagt cgaagtacct
     3121 ccatctctcg ttgagtaaac tcgcaggagc ttgctttgcc gatctgtact tcgggaactt
     3181 tacccggcca taccgattcg ccgctcattg tacgatccat aatatccaac aattcatttt
     3241 cacttacttc cttataccaa aaactgtccg catttgcttc tcttgctttg gttataaagc
     3301 ttgaatcggc catggaagtc actataataa ctttaatatc gggaaagtat cgctttataa
     3361 cggaacaggc cttaatgccg ctttcgttgt tctcggtaca aacatccata agtataaggt
     3421 cgacaggata acacatgcat gttatttccg ccattcccgc attagttata gaggctacaa
     3481 gactatagcg gtcactgctt ttgatatagc cttccatgca atcgcgaggc attattttat
     3541 cttccacaat aagtacatta accatagatt atttctcctt tccgggcaaa atcactgtca
     3601 atctgtatgt aggactgctc tttattctca tagtaccgcc cgccttttct atacgctttc
     3661 ttaaatcgga caaacctccg ccctctacaa ctttcctctc gggcggcttt ccgttatttt
     3721 caatccatac aacaagtgtg ctttttactt caaatatcct tactttcatt tcattaccgc
     3781 cggcatgacg taccgtattg gtggcacatt ccctcatagc ggtagtaata aggtaacttt
     3841 gttcccggtc ctcaggtaaa ttaccgtcaa gaatgatttt taagcctatc cctgcggcgg
     3901 aattaataag gttatggagc atttcctccg acctgcttga ggtttttttc tctatatttg
     3961 tataatcact tgccaccgca tcggctatac cgaaaacatt aagggtgctt ctccatattt
     4021 tcccggcttc cttaagttcg gtaagtccgc cttcacggag caccttatgt gtggcaagca
     4081 accgtaaacc tatatcgcta tgaatacgca ttttgattgc aaaagcttct tcctctctga
     4141 cggtattgtc caactccgca taaagcttcc ttgcccgctt attcacttct tccaattcgc
     4201 gatttttttg ctccagctct gtttttttgg catacagttc ggtcaaagaa agtgcctgta
     4261 tctgcgtata tgttttgccg ccgacaacgg taaccgtact ttccctaaac tcccatatcc
     4321 gaccgtcggg gaaacgaaaa gtatcatttg ttccctcaac atgtgtaacg gtatccgtag
     4381 gtgtttgtaa tgcctctctg agctcaaaaa tgtgttgcaa acctgttccg agcaatatgc
     4441 aacataatct gtgcatttgt ctgttacaca atacaatcat acctttgctg tcggcaaag
//
LOCUS       DAOPWC010000074         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_19478_length_4485_cov_3.176524, whole genome shotgun sequence.
ACCESSION   DAOPWC010000074 DAOPWC010000000
VERSION     DAOPWC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19478_length_4485_cov_3.176524"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKJ65_02500"
     CDS             <1..324
                     /locus_tag="PKJ65_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40739.1"
                     /translation="GADITISIKGTDGLATKLDLIFEKHGLFDCDYASTQAAGNDFIQ
                     LQQGNFTYTAGSDRITAGPAFNELSIGSEGLRGSNPPIENSFTVYFTGMTPSVHTVRI
                     RGDRI"
     gene            321..1910
                     /locus_tag="PKJ65_02505"
     CDS             321..1910
                     /locus_tag="PKJ65_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40740.1"
                     /translation="MKELIKERIRRSDDIIENQLNAQCTDPDSVYYGNVPDRTAGYNE
                     PTRSILVAATYITRYCHPLSRFYKNQLLLQRALIGLKHGLKHMHPDNTMDLTATNPHD
                     PTSVAFSVRTIVPAFRMLKNYMQKKSSPSALEVKVYNTIINYLTKSADGMVGKGFHTP
                     NHRWVVASGLAMVMNILDMPELMSEINKYLDEGIDCDEYGEYAERSISIYNLTNNESL
                     IILARELNRPEFLEHVRRNLYMSMKYIEPDGTLYTLNSSRQDVGTRIYPFTLFEGYMV
                     MAHIDRNPQFAYMAKVIFDMCSNNPIDTGELGYYCFTAHIIRNMEYSEFFDGEVAILP
                     FDYTHYDAFFPYSDIARMRDGDTSMTVLAKNVMPVKYMYRFLGIYMMFLSDKYMEFDR
                     IEKTHAGYRLYGNDDKTEVILDCVFQPDKATISVETKADQPYPCAAVLMFDKEGFLQG
                     DGYSLRARTGKNLHVKNGKFTYLPSVRYIPNALTVESDFTGDEYSKICMNKVLPTDKN
                     SFTVFLTAEAPFRKTITIKKE"
     gene            complement(1913..3169)
                     /locus_tag="PKJ65_02510"
     CDS             complement(1913..3169)
                     /locus_tag="PKJ65_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40741.1"
                     /translation="MRYKIEKREREYLRDYAKKQLEISKLPVMNERVKHWYNHNENRK
                     GKPMIVIELNSFKNDVRSPLKTQSDFARRIESAIQSNLLVHELLDDDQVCPDFLSFNW
                     DITYKEFGVDIPVIKSKDASGREIGFEYIHPITDLERDFPILKPFTFSVDRENSLRRL
                     QAAEDILGDILNVELENTFHQWNHMITYKVIKLMGMENWMIAMFETPDTVHKLMDYIT
                     SNLLAYQQWMEDEGLLTMNNSNHYTGAGSRGFTHELKKPADGRVRLKDLWINVNSQES
                     TGISPDTYREFVYPYIDRIAREFGMVYYGCCEAVHSLWDVCLKDLSNLRKVSVSPWCD
                     EEFMGERLRGTNIIYSRKPSPHFLGVGYDFDDDEYKKYMQNTLKAAKGCNLEIIFRDV
                     YTLGGNPSKAKRAVRILRELIEEYWE"
     gene            complement(3296..4450)
                     /locus_tag="PKJ65_02515"
     CDS             complement(3296..4450)
                     /locus_tag="PKJ65_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40742.1"
                     /translation="MSEDILNKFKKIHEEKKSTDTGKVLKIGIIGTGWIAEPHVNAYL
                     KCPDAKIVACADLIDGKAAAFCKKMGLEDVCLYRSHKELLDNEELDAVSVCTYNCTHA
                     ECAIYALEKGVHVLLEKPMCVTLDEAVAIVRAEKASGKVLSIGFQPRFDENMKMIKKI
                     VQNGELGDVYYIQTGSGRRRGIPTPFGTTFIQKDTGGIGALGDIGCYSLDMVLNAVGY
                     PKPLTVSGYKSDFFGKRPSYYPKHPEYAKVFGVDDFAAAFIRLEGGIVLDFRISWAMN
                     MDTSGDTLILGTKAGLRIPSTECWNGTVGGPMTLYHEISGKQVETVIPVLKTDFNLFD
                     MKIRTFLDAVKEGTPSPIPSSQILYNQAIIDGINKSAELGREIEIKIPKI"
BASE COUNT         1274 a         1015 c          840 g         1356 t
ORIGIN      
        1 ggagcggata taaccatatc cattaaggga acggacggtt tagcaaccaa gctggacctt
       61 atattcgaaa aacacggttt gtttgattgc gactatgctt ccacacaggc tgcaggcaat
      121 gattttattc agcttcagca aggcaacttc acttataccg caggaagcga ccgtataacg
      181 gcaggtccgg catttaatga attatccatc ggaagtgaag gtctgagagg tagcaatccg
      241 ccgatagaaa actcctttac cgtttatttt acgggtatga cgccgtcagt acataccgtc
      301 aggataagag gtgacagaat atgaaagaac tgattaaaga aagaataaga cgttccgatg
      361 atattataga aaaccagctg aatgcgcaat gtaccgatcc cgattccgtg tattacggaa
      421 atgtaccgga tcgtacggcg ggatacaatg aacctacacg atctatactg gtagccgcaa
      481 cctatattac cagatactgc catccgctat cccggttcta taaaaaccaa ctgctcttac
      541 agcgtgcttt aataggtttg aagcacgggc tgaaacatat gcatccggat aatacgatgg
      601 atcttacagc caccaatccg catgacccta catccgttgc attttccgta aggacaatcg
      661 taccggcttt tcgtatgctt aaaaactaca tgcaaaaaaa atcgtcacct tccgctttgg
      721 aagtgaaagt ctacaacact ataataaatt atctgacaaa atcggcagac ggaatggtag
      781 gtaaaggatt ccacaccccc aaccacagat gggttgttgc cagcggtctt gcaatggtaa
      841 tgaatatatt ggatatgccg gagctgatga gtgaaataaa taagtatctt gatgaaggaa
      901 ttgactgtga tgaatacggc gaatatgcag agcggtccat atcgatatat aacctgacaa
      961 ataacgagtc gcttatcata ttggcccgtg agttgaacag gccggaattt ttggagcatg
     1021 taagacgtaa tctgtatatg tctatgaaat atatcgagcc ggacggaacc ttatataccc
     1081 ttaattcctc cagacaggat gtcggaacca gaatctatcc ttttactctt tttgaaggct
     1141 atatggtaat ggcccacata gacagaaatc ctcaatttgc atatatggcc aaagtgatat
     1201 tcgatatgtg cagcaacaat cctattgaca caggtgagct gggctattat tgcttcacgg
     1261 cacatatcat acggaatatg gaatattctg agttttttga cggggaggtt gccattttgc
     1321 catttgatta tactcattat gacgccttct ttccctattc cgacatagcc cgcatgagag
     1381 acggcgatac ctccatgacg gttcttgcaa agaatgttat gcccgtaaaa tatatgtaca
     1441 gattcttagg tatatatatg atgtttttgt cggataagta tatggaattt gacagaattg
     1501 aaaaaacgca cgcaggatac agactgtacg gaaacgatga caaaaccgaa gttatattgg
     1561 actgcgtatt ccaacccgac aaagcaacaa tatccgtaga gacaaaagcc gatcagccgt
     1621 atccctgtgc ggcggttctt atgtttgaca aagaaggatt tttgcaaggt gacggttatt
     1681 cactccgagc aagaacggga aagaatcttc atgtaaagaa cggcaaattc acttatttgc
     1741 cttcggtcag atacattccc aatgctctta cggtggaaag cgattttacc ggtgatgaat
     1801 attcaaaaat atgtatgaat aaggttctgc cgaccgacaa aaactctttc accgtttttt
     1861 tgacagcgga agcccctttc aggaaaacta taacaattaa aaaagaatag tgttattccc
     1921 agtattcttc tatcaattcc cgtaagattc ttacggctct tttggctttg gaagggttac
     1981 cgcctaaagt atatacatcc ctgaatatta tctctaaatt acaacctttt gccgccttta
     2041 aagtgttttg catatacttt ttatactcat cgtcatcaaa atcatatccc actcccagaa
     2101 aatgaggtga aggttttctg gagtaaatga tatttgtccc tcttaatctt tcacccataa
     2161 actcttcatc acaccacgga gaaaccgata ctttgcgtaa attggataaa tccttcagac
     2221 aaacatccca aagagaatgt acggcctcac aacatccgta atataccatt ccgaactctc
     2281 ttgctattcg gtctatatac ggatacacaa actccctgta tgtatcggga gatattccgg
     2341 tagattcctg tgaattaaca tttatccata agtcttttaa tcgtacacga ccgtcagcgg
     2401 gtttctttaa ctcatgagta aatcccctgc ttcccgctcc ggtatagtga ttactgttat
     2461 tcatggtaag caatccttca tcctccatcc attgttggta tgcaagcaga ttggaagtta
     2521 tataatccat aagcttatgc accgtatccg gtgtttcgaa catggctatc atccagtttt
     2581 ccattcccat aagttttata actttatagg taatcatatg attccattgg tgaaaagtgt
     2641 tttccagttc tacattgagt atatctccca atatatcctc ggcggcttgc agccttctta
     2701 acgaattttc tctgtctacc gaaaaagtaa aaggtttgag tatgggaaaa tcccgctcca
     2761 gatcggttat cgggtgtata tattcaaaac ctatctctct gcctgaagca tctttggatt
     2821 ttataaccgg tatatccact ccgaactctt tgtatgttat atcccaattg aaggaaagaa
     2881 aatccggaca aacttggtca tcatcgagaa gttcatgaac cagcaggttg ctctgaatag
     2941 cggattctat tcttctggca aaatccgact gtgtttttaa aggagaccgg acatcatttt
     3001 taaacgagtt aagctctatc actatcatag gcttaccttt tctgttttcg ttatgattgt
     3061 accaatgttt aacccgctcg ttcattaccg gcaacttgga aatctccaac tgcttcttgg
     3121 cataatctct taaatattct ctttcccgtt tctctatctt gtatctcatg atttccatac
     3181 tccgctgtta aatataatat ccaatcaaat actgttaatc aataaaattc ataaaacatc
     3241 atacgacaca aaggcgcctt ttggcgcctt tggactgaac ttaaacgttt tgttattaaa
     3301 tcttaggtat cttaatttca atttcccttc ccagttcggc ggatttattg attccgtcta
     3361 ttatagcctg gttatacagt atctggctgg aaggtatcgg agacggagtt ccttctttta
     3421 ctgcatccaa gaaggtcctt atcttcatat caaaaagatt gaaatcggtc tttagtaccg
     3481 gtatgacggt ctccacctgc ttgccgctta tttcatgata cagcgtcatg ggaccgccga
     3541 ccgtaccgtt ccagcattcg gttgaaggaa ttctcaatcc ggcttttgta cctaaaatca
     3601 atgtgtctcc cgatgtgtcc atgttcattg cccaggaaat acggaagtcc aatacaatac
     3661 ctccttccaa tcggataaat gcagctgcaa aatcgtccac tccgaaaacc tttgcatatt
     3721 ccggatgttt gggataatag ctcggtcgtt ttccgaagaa gtccgactta tatcccgaaa
     3781 cggtaagcgg tttcggataa cctacggcat tcaataccat atccaaagaa tagcacccta
     3841 tatctcccaa agctcctatt ccgccggtat ctttctgtat gaaggtggta ccgaaaggag
     3901 tgggtattcc tcttcttctg ccgctgcctg tctgaatgta gtagacatcg cccagttctc
     3961 cgttctgcac tatcttttta atcatcttca tgttctcgtc aaatctcggc tgaaaaccta
     4021 ttgagagaac tttacccgat gctttttccg cccttactat tgcaaccgct tcatccagcg
     4081 ttacacacat gggcttctcc aagagtacat gcacaccttt ttccagagca tatattgcac
     4141 attccgcatg agtacagtta tatgtgcaaa cacttaccgc atccaactct tcattatcca
     4201 gaagttcttt gtgacttctg tacagacaca catcttctaa ccccattttc ttacagaaag
     4261 ccgcggcttt tccgtctatc aagtcggcac atgccactat ctttgcatcg gggcatttta
     4321 agtacgcatt gacatgaggt tccgctatcc aaccggtacc tatgatacct attttaagca
     4381 ccttaccggt atcggtactc tttttttcct cgtgtatctt cttgaatttg tttagaatat
     4441 cctctgacat atttccctcc ttttttacca aatcaggttt tttaa
//
LOCUS       DAOPWC010000075         4367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20229_length_4367_cov_3.764610, whole genome shotgun sequence.
ACCESSION   DAOPWC010000075 DAOPWC010000000
VERSION     DAOPWC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20229_length_4367_cov_3.764610"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKJ65_02520"
     CDS             <1..537
                     /locus_tag="PKJ65_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40743.1"
                     /translation="SLYYALLDWKNVVDKENLHFHTGITCTAVACMRMADLMKKILEK
                     LDIKHREHHSLSYHEIGGWDVSVFDKEGIAELTYDIAKYADKEGRYHICFDYTDGKSD
                     ISVKSISGLCNNIEKAHVLYDNTSSLKRLNNDEPWYEVFIDIECTGRDQEKILKIILE
                     RATDNKNKCNGIVGIRKI"
     gene            complement(538..1566)
                     /locus_tag="PKJ65_02525"
     CDS             complement(538..1566)
                     /locus_tag="PKJ65_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40744.1"
                     /translation="MNSRERVHAAIHFQPVDKAPLQYNYTPVGYFEHGEKLNELYASV
                     PDDFGPFQKKEIPALTKDQFDENGNYHEFKKDEWGTVWEYRIYGIAGIPHIYPLEDMS
                     NLKDYKMPEQSEIGSEQFLQDKRDAVLHKKEYYLSRFAGTMIEKLKALCPDENVYCDI
                     AEDTKEINKLADMIVEYYEKKIQYLLAIDADGFYFGDDYGTERSMILSPALWRRFFKP
                     RWDRLFEPIRAAGKNIHFHSCGYVLPILEDLKELGVHSIWPQLPAYNIEEFAKYCRSL
                     KLAVAIHTDRGKVMTFGTPSEVRDLVKREYETFRMHEGGSWFYVECDNGFPFENMEML
                     VRTIAEYR"
     gene            complement(1612..3357)
                     /locus_tag="PKJ65_02530"
     CDS             complement(1612..3357)
                     /locus_tag="PKJ65_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNZ40745.1"
                     /translation="MSRLGYTDKPVENLTEDSFGIGTYINGLCQFIVSCDTPMTISIQ
                     GDWGSGKTSMMNMIKQQIEDKVYPIWFNTWQYSQFNMGNDLCLSFLENLITKLQSKAE
                     TATSKILETSLKILKIGAKTAISSVGLDGAAIVNVFDKQDSDIAKAVNDLKDAFQESV
                     NNKLKDNKKDRVVIFVDDLDRLNPGKAVELLEVLKVFLDCENCVFILAVDYSVVTAGV
                     RQKFGNLVDEDKGKSFFDKIIQLPFKMPVAQYDINKYIYDMLKKMNMRADSRIVQDYK
                     QLIRNSIGFNPRSIKRLFNTYQLLNLISDSKKTSVREDLHQRLLFAIICMQMTYEHLY
                     AFIVTSAKNDELTGKGLRTLADPEVVRNYKGLPEELRLIGKDGKIDEDELIRMCAFMK
                     TFVSIVDIDKDRLLSDEELNDMKEILFFSSVTSIMEEAKTGTGMEADQKIRARNRAII
                     DDVDANLTNLIGDFEIWQPLKSESDTKMSDVRCSINLITVSGIPFKYEFSISSKTLQD
                     ICLFIKFTCVQENLERLKEFFGPNPLDNEIPPVINDKGYEYVCMNIPEDKAVEVITEK
                     VKEVHGKLKTKGYIV"
     gene            complement(3516..>4367)
                     /locus_tag="PKJ65_02535"
     CDS             complement(3516..>4367)
                     /locus_tag="PKJ65_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNZ40746.1"
                     /translation="ADVFAYAAASIKKAMDITKKLDGQGYVFWGGREGYETLLNTDMK
                     LELDNLARMMHMAVDYKKKIKFNGPFYIEPKPKEPTKHQYDFDAASSIAFLRKYGLEK
                     HFKLNIEANHATLAGHTFEHELRTAAINDMFGSIDANQGDMLLGWDTDQFPTDVYNAV
                     YCMLEVIKAGGFTTGGLNFDAKQRRGSNTPEDTVIAYIAGMDTFALGLKIAYAIIDDG
                     RIDDFVKERYKSYTHGIGADILNGKTDFEKLTRYALSNGEITTNTSGRQEMLESIINS
                     IMFGNRF"
BASE COUNT         1186 a          929 c          808 g         1444 t
ORIGIN      
        1 agcttgtatt atgctctttt agattggaaa aatgttgtcg ataaagagaa tctgcatttc
       61 catacgggaa taacatgtac ggccgtcgca tgtatgagaa tggccgacct tatgaagaaa
      121 atactggaaa aactggatat aaagcaccgg gagcatcatt cactttctta tcatgaaatc
      181 ggcggctggg acgtgtctgt gtttgacaaa gaaggaattg cggagctgac gtatgatatt
      241 gcaaagtatg ccgacaaaga aggccgatat cacatttgtt tcgattatac ggacggtaag
      301 tccgatatat cggtaaagag catatcgggt ttatgcaata atattgagaa agcgcatgtt
      361 ctttacgaca atacttcttc tctgaaacgg ttgaataacg atgagccttg gtatgaggta
      421 tttatcgata ttgagtgtac aggaagagac caagaaaaaa tactgaaaat aatattggaa
      481 agggctacag acaataagaa taagtgtaac ggtattgtag gtataaggaa gatatagtta
      541 tcgatattcg gctattgtac gaacaagcat ttccatattt tcaaacggga aaccgttgtc
      601 gcattccaca taaaaccacg atcccccttc atgcatacga aatgtttcgt attctctttt
      661 tacaaggtcc cgtacttcgg aaggcgttcc gaaagtcatt acttttcctc tgtcggtatg
      721 aatggctacg gcaagtttta atgacctgca gtattttgca aactcctcta tattgtaagc
      781 gggaagctga ggccatatgg aatgaacgcc taattctttt aagtcttcca aaataggaag
      841 aacatacccg caactgtgga aatgaatatt ttttccggct gctcttatag gctcaaaaag
      901 tctgtcccat ctgggcttaa aaaatcttct ccaaagtgcg ggactcagaa tcatacttcg
      961 ttcagttccg tagtcatcgc cgaaataaaa gccgtcggca tcaatagcca gaagatattg
     1021 aattttcttt tcgtaatatt caactatcat atcggcaagt ttgtttattt cttttgtgtc
     1081 ttctgctata tcgcagtaca cattttcgtc cgggcacaga gccttcagct tttcaatcat
     1141 agtgcctgca aatctggaca gataatattc ttttttatga agaacggcat cccgcttatc
     1201 ctgcaaaaac tgctcgctgc cgatttcaga ctgttcaggc attttgtaat cttttaaatt
     1261 tgacatatcc tcaagaggat atatatgagg aattcccgct ataccgtata tcctgtactc
     1321 ccagaccgta ccccattcgt cttttttgaa ttcatgataa ttgccgtttt cgtcaaactg
     1381 atcttttgtg agtgcgggaa tctctttttt ttgaaaaggt ccgaaatcat cgggaacgga
     1441 agcataaagt tcgttaagct tctctccgtg ctcgaaatat cccacgggag tgtagttata
     1501 ttgcagcgga gctttatcta cgggctggaa atgtatggct gcgtgaactc tttcacggct
     1561 gttcataggg tacctcttct tgtgatactt atcaaatata aagtcggcat gttaaactat
     1621 ataacctttg gttttcagtt ttccgtgtac ttctttgacc ttttcggtaa tgacttcaac
     1681 cgctttgtcc tcgggtatat tcatacagac atattcatat cccttgtcgt tgataacagg
     1741 cgggatttca ttatccaacg gattgggtcc gaaaaactct tttaatcttt ccagattctc
     1801 ctgtacacat gtgaatttaa taaacaggca tatatcctgc agggttttgg aagatatgga
     1861 aaactcatat ttaaaaggta taccgcttac agtaataaga ttaatggaac atcttacatc
     1921 ggacatttta gtgtcgctct cggatttcag cggttgccat atttcaaaat ctcctatcag
     1981 attggtaagg tttgcgtcta cgtcatctat gatagctctg tttcgcgccc ttatcttctg
     2041 atccgcttcc ataccggtgc cggtctttgc ctcttccatt atggaagtaa ccgaggagaa
     2101 gaaaagtatc tctttcatat cattcaattc ctcgtcggat aagagtctgt ctttgtctat
     2161 atccactatg gagacaaatg ttttcatgaa tgcacacatt cttatgagtt cgtcttcatc
     2221 tatcttcccg tctttaccta ttaaacgcaa ttcttccgga agacctttgt agttgcgaac
     2281 cacttcggga tcggcaagtg tccgaaggcc ctttcccgta agttcgtcat ttttagcact
     2341 tgttacaatg aaagcataca gatgctcata ggtcatctgc atgcagatta ttgcaaaaag
     2401 cagtctttga tgtaaatctt ccctgacgct ggttttcttg ctgtccgata taagattcag
     2461 caactgatat gtgttgaaaa gtctttttat gcttctggga ttgaatccta tggaatttct
     2521 tatgagttgc ttatagtctt gcactattct ggaatccgct ctcatattca tttttttcag
     2581 catatcgtat atgtacttat ttatatcata ctgcgccacc ggcatcttaa acggaagctg
     2641 tattatctta tcaaagaagc tcttgccttt gtcttcatcc acaagatttc cgaacttctg
     2701 ccgtacaccg gctgttacca cgctatagtc gacggctaaa atgaacacac agttttcaca
     2761 gtctaaaaac actttaagta cttccagaag ttccaccgct ttgccgggat ttaatctgtc
     2821 cagatcatcc acaaatatga caacacggtc ttttttattg tctttaagtt tgttgtttac
     2881 gctttcctgg aatgcgtctt tcaaatcgtt taccgctttt gctatatccg agtcttgctt
     2941 atcaaataca ttcactatag cagcgccgtc aagaccgacc gaactgatgg ctgttttggc
     3001 tcctatcttc aatatcttta aactggtttc cagaattttg gaagttgcgg tctccgcttt
     3061 ggattgcagt ttggttataa ggttttccaa aaaagataaa cacaaatcat tacccatatt
     3121 aaactgtgaa tattgccagg tattaaacca gataggatat actttatcct cgatttgttg
     3181 ttttatcata ttcatcatac tggtttttcc gcttccccaa tcaccctgaa tggatatggt
     3241 catcggtgta tcacagctta caataaactg gcataaaccg ttaatatatg tacctatacc
     3301 aaaagaatct tccgttaaat tttctaccgg tttatccgta tatcccaacc tgctcatttg
     3361 ctaccccctt ttaaaagcta atagaattcc gatattctta atccgatttt ttatacataa
     3421 tgattataca caaaaaccat gaataatatc aattaatcat gggttaagta tcaaatatta
     3481 tttatgctat tctttcaaac tccgattacc gttgcctaaa aacgattacc gaacattatg
     3541 gagtttataa tgctctccag catttcctgt cttcccgaag tattggttgt aatctctccg
     3601 ttggacaatg catacctggt cagtttctcg aaatcggttt ttccgtttaa gatatctgca
     3661 cctataccgt gagtgtaact cttgtaacgc tccttaacga agtcgtcaat tcttccgtcg
     3721 tctataatag cataagctat tttaagacct aatgcgaatg tatccatacc ggcgatatat
     3781 gcaataacgg tatcttcagg tgtatttgag cctcttcgct gttttgcatc gaaattcaaa
     3841 cctccggtag tgaatccccc cgcttttatt acttccagca tgcaatatac tgcattgtat
     3901 acatcggtag ggaactgatc ggtatcccac cccagcaaca tatctccctg atttgcatca
     3961 atggatccga acatatcgtt tattgccgca gttcttaact catgctcgaa tgtatgtccc
     4021 gcgagagttg catgattggc ttcaatgttt agtttaaaat gcttttccaa tccgtatttt
     4081 cttaagaatg ctattgagga tgccgcatca aaatcatact gatgttttgt aggctccttg
     4141 ggcttgggtt ctatgtagaa gggaccgttg aatttaattt tcttcttgta atccacggcc
     4201 atatgcatca tacgtgccag attatccaac tccagtttca tgtcggtatt cagaagagtt
     4261 tcatatcctt cgcgtccgcc ccagaaaaca tatccctgtc cgtcaagttt cttggttata
     4321 tccatcgcct tttttatgga agctgccgca taagcgaaaa catcggc
//
LOCUS       DAOPWC010000076         4363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20255_length_4363_cov_3.075673, whole genome shotgun sequence.
ACCESSION   DAOPWC010000076 DAOPWC010000000
VERSION     DAOPWC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20255_length_4363_cov_3.075673"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKJ65_02540"
     CDS             <1..726
                     /locus_tag="PKJ65_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40747.1"
                     /translation="IYPIYDGEIVYIKKDTSAQLGHIVVKSDIGYEEPVYIEYLHVIP
                     VRGLDENDYVYTSIPIATIDEYNRYDSHLHLGRVNEDRTLHYQLYDLFSDVLRWNNGS
                     DLDVFSYPVFNHETNIFSVTAYVSSDTENLDYYGGYGRFSLKYITFHYSVNNGTWKSF
                     NIAEHDKDFRYSFNIKDMTGSKSKDVVKYYITATRDNQSNTDTTFKNAPYTVAYYPAY
                     YAHPSPALTKEQADRIALSLTIK"
     gene            complement(719..2479)
                     /locus_tag="PKJ65_02545"
     CDS             complement(719..2479)
                     /locus_tag="PKJ65_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40748.1"
                     /translation="MLKDMKFIFRLMGKNKGRYSVLLATWSIIGSLVDMTFSYALKLL
                     TDFFVYKEKENVLLLILVFSASIVSSILFCVFFHLHNKVKEDILRDIRMMTFREVQKL
                     PSSYFKGTHSGDLVSRFNADTQSVITVFVEFEHIFESAIVLLMRIPFALLLDFRFGAI
                     LLGFSVFTGVLNVLFIKPLREKHKNILKDKASLSKQASEVVTGFTVARMFSLSTFLRS
                     RFEKSVQDSFDSGWKAAKTYTLQSGLNTIIWNFSHYLSTILGALFIINGTLEAGNYMA
                     ISTSTGIGWSVSILITALPNLQKAFAGSNRLKELYDEPKEPELYDIEGTETNMGIEMS
                     KVGFAYEIRDNEEKEENSKDKENNFRISGLSLDIEQGKTLALVGDSGGGKSTIAKLIL
                     GLFKIDEGNMQINGKAYKDYTLEQLRESIGYVPQDAYIFNGTISENIKYGKPDATEEE
                     IINAAMLANAHDFILAQPDGYETYVGERGIRLSGGQRQRIAIARAIIKNPSILLLDEA
                     TSSLDSESEKMIQDALDRLIKTKTSLVIAHRLSTIMNADRICYIKDGYVAEQGTHAEL
                     LAKKGLYHELYYREFAKEAI"
     gene            complement(2472..4244)
                     /locus_tag="PKJ65_02550"
     CDS             complement(2472..4244)
                     /locus_tag="PKJ65_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40749.1"
                     /translation="MAKKDKELSQKKEKTDVKYVIKTMVKLYKESAEHKKWAFGRWFG
                     GVVSGILAVVIFFVTGSVLDVALSGDYNRLYTLIYALSAIFVIRFILQFVNPLIASQY
                     ELRTHKTLAMNVYDKIDKLKMGYYENVHTADTISTLVDDVEKIKTFMGNTVAGFLSYN
                     PVSFILSMFFLIRINWKLTIFSLTVIPLIMYLIDKASKPLREVKNKIQEYTAILNSYI
                     RDFVEGNDIYKSFNMHKSHTAKVQQSCQDIAEESYKNSYVMTKNRALTILMMIIPQGV
                     CMFASMYFIARNELTIGQYVIFSNLLWPLMQTFRTAGMGWADIVGYTGTAERYFKFLE
                     TKEERCDGEDFGTQDSDIIVEFKNVGFSYHEDIPLLKDVSFKLHKNSRMALCGVSGSG
                     KSTIFKLICGYYENFTGEIYVFGHSIRSWNLKALRKYMTVVTQDVYLFDDDIINNIKM
                     GNLEATDEMVAEASKKAYIEQLVKDVGERGAKVSGGQKQRIAVARALLKDAPLIMLDE
                     PTSALDTKSEYYVEKSIENLKKGRSVLVIAHRLTTIIDSDCIILLEDGTVKEQGTHEE
                     LIRKKKRYAELYERQLVEEEAHNA"
BASE COUNT         1271 a          957 c          721 g         1414 t
ORIGIN      
        1 atttacccta tatatgacgg tgaaatagtt tacattaaaa aagacacatc ggcacagttg
       61 ggtcatattg tggttaaatc ggatatagga tatgaagagc ctgtatatat agaatacctt
      121 catgtgatac ccgtccgggg tttggatgaa aatgattatg tctatacttc cattcccatt
      181 gctacaattg atgaatataa caggtatgat tcacatcttc acttaggaag agtgaatgaa
      241 gacagaacat tacattatca actctatgat cttttttccg atgttttaag atggaacaac
      301 ggctccgatt tggatgtgtt cagttatcct gttttcaacc atgaaaccaa tatcttttcc
      361 gtcaccgctt atgtatcgtc ggatacggaa aaccttgatt attacggcgg ttacggacgt
      421 ttctctttga aatatataac ttttcattat tcggtgaata acggcacctg gaagagtttt
      481 aacatagcag agcatgacaa agacttcagg tattcattca acataaaaga tatgacaggt
      541 tctaaaagta aggatgttgt aaagtactat ataaccgcaa caagggataa tcagagtaat
      601 accgatacca catttaaaaa tgcaccgtat accgtagcat actaccccgc atactatgca
      661 catccgtcgc ctgcccttac aaaggagcag gcggatagaa ttgctttatc tctaacaatt
      721 aaatagcttc tttggcaaac tctctgtaat acagttcgtg atacagacct tttttagcca
      781 acagctccgc atgtgtaccc tgttccgcta cataaccgtc ttttatataa catatacggt
      841 cggcattcat gatggtggaa agcctgtgag ctattacaag tgatgttttg gttttgataa
      901 gtctgtccag cgcatcctga atcatttttt ccgattcgct gtccaaagag gatgtagctt
      961 cgtcaagaag taaaatggaa ggatttttaa ttatagccct tgctatggca attctttgcc
     1021 tttgaccgcc cgataacctt attcccctct ctcctacata tgtttcgtat ccgtccggtt
     1081 gcgcaagtat aaagtcatga gcatttgcca gcatggccgc attaattatc tcttcttccg
     1141 tagcatccgg tttaccgtac ttaatatttt cacttatagt gccgttgaag atatatgcgt
     1201 cctgcggaac atacccgatg ctctctctca actgctctaa tgtgtaatct ttataggctt
     1261 taccgttaat ctgcatgtta ccttcatcaa tcttaaaaag acctaagata agttttgcaa
     1321 tagtactctt accgcctccg ctgtcaccta ccaatgccag tgtcttgccc tgctcaatat
     1381 ccagacttaa accggatatg cggaagttat tctctttgtc cttactgttt tcctctttct
     1441 cttcattatc tctaatctca tacgcaaaac ccacttttga catctctata cccatattag
     1501 tttccgtacc ttcgatatca tataattcag gctctttagg ttcatcatac aactctttca
     1561 atctgttgga gcctgcaaag gccttttgta aattgggtaa tgcagttata agaatggata
     1621 ccgaccagcc aattccggta ctggtggata tagccatata atttcccgct tccagagtac
     1681 cgttaatgat gaataaagcg cctaaaatag tgcttaaata gtgcgaaaag ttccatatga
     1741 tggtattcag cccactttgc agagtatagg tttttgccgc cttccatccg ctgtcaaaac
     1801 tgtcctgaac actcttttca aatcgcgaac gcaagaaagt actcaaggaa aacattcttg
     1861 caaccgtaaa gccggtaacc acttcggaag cctgtttact taatgacgct ttgtctttta
     1921 aaatattttt atgcttttct cttaacggtt taataaacag aacattcaaa acaccggtaa
     1981 aaacggagaa gcctaagaga attgctccga accggaaatc caacagcagc gcaaaaggta
     2041 ttctcattaa tagaacaatg gcagactcaa atatatgctc gaattcgaca aataccgtta
     2101 ttacactctg ggtgtctgca ttaaatctgg aaacaagatc tccgctgtga gtacccttaa
     2161 agtaggaaga aggaagtttc tgaacctctc tgaatgtcat catccttata tcccgcaata
     2221 tatcttcctt tactttattg tgcagatgga agaatacaca aaagagtatg ctgctgacaa
     2281 tacttgcaga aaagaccagt ataagcaata atacattttc tttttcttta tacacgaaga
     2341 aatcggtaag caacttaagc gcataagaga aggtcatatc cacaagactg cctattatac
     2401 tccatgtcgc taacaaaacc gaatatctgc ctttattttt ccccatcaaa cggaaaatga
     2461 acttcatatc cttaagcatt gtgtgcctcc tcttctacca actgtctttc gtataattcc
     2521 gcatatcgct ttttcttcct gataagttcc tcatgggttc cctgttcttt aacggtaccg
     2581 tcttcaagaa gaataatgca atcggaatct ataatggtag ttaatctatg agcaataact
     2641 aaaaccgaac gtcctttttt taagttttct atggatttct ccacatagta ctcggatttg
     2701 gtatccaagg cacttgtagg ttcgtccaac attatcaaag gtgcatcttt aagtagagct
     2761 ctggctaccg ctattctctg cttctgaccg cccgatactt ttgctcctcg ttctccgaca
     2821 tctttaacca gttgttctat gtatgccttt ttagatgcct ctgccaccat ttcatctgtg
     2881 gcttccaaat tacccatctt tatgttattg ataatgtcat catcaaacag gtatacatcc
     2941 tgagttacaa cggtcatata ttttctcaat gcttttaagt tccaagacct gatactatga
     3001 ccgaaaacat atatttcgcc agtaaaattc tcataatacc cgcagataag cttgaatatg
     3061 gtgcttttac cgcttccgct cactccgcat aatgccattc tggagttttt atgcaatttg
     3121 aaactgacat ccttaagtaa aggtatgtct tcatggtagg agaaaccgac atttttgaac
     3181 tccacaatga tatccgaatc ttgggtaccg aaatcctcac cgtcacatcg ctcctctttt
     3241 gtttcaagaa atttaaaata tctttccgca gtaccggtat aacctactat atccgcccat
     3301 cccatacctg ccgttctgaa agtttgcatg agaggccaaa gcagattgct gaatattaca
     3361 tactgcccta tcgtcaattc atttcttgca ataaaataca ttgatgcaaa catacaaaca
     3421 ccttgcggaa taatcatcat cagaattgta agcgctctgt tcttggtcat aacatagctg
     3481 tttttatatg attcctccgc tatatcctga caagactgtt gaacctttgc cgtatgagat
     3541 ttgtgcatgt taaatgattt gtatatatcg ttaccttcaa caaaatcccg gatatatgaa
     3601 tttaatatag ccgtatattc ctgtatctta ttctttactt cccttaaagg tttggaggct
     3661 ttatcaatta gatacattat taacgggatt acggttaagg aaaatatagt cagtttccaa
     3721 ttaatcctga taagaaaaaa catggaaagt ataaaggata cgggattgta ggaaagaaat
     3781 cccgctaccg tattacccat gaatgtcttt atcttttcca catcgtctac caatgtgctt
     3841 atggtatcgg cggtatgtac attttcataa tatcccatct taagtttatc tatcttgtca
     3901 tatacattca tggcaagagt tttgtgtgtt cttaactcgt actgtgaagc tatcaaggga
     3961 ttaacaaact gaagaatgaa tcttattaca aatattgcag ataaagcata tataagagta
     4021 taaagcctgt tataatcgcc cgacaaagct acatccaata ccgatcccgt tacaaagaat
     4081 atgacgaccg ccaatattcc cgatactaca ccgccgaacc atcttccaaa agcccatttt
     4141 ttatgttccg cagactcttt gtataacttt accatcgttt tgatgacata tttcacatcg
     4201 gtcttttctt tcttttgaga taattcttta tctttttttg ccatatttgc cctcacacaa
     4261 aatccattgt tatctgtcca tggtcggttt taatatccgc tgctgctatt ataaccgatt
     4321 tatatgcata acggctttca ccttttgcat aaataatcct tcc
//
LOCUS       DAOPWC010000077         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20357_length_4344_cov_3.930287, whole genome shotgun sequence.
ACCESSION   DAOPWC010000077 DAOPWC010000000
VERSION     DAOPWC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20357_length_4344_cov_3.930287"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKJ65_02555"
     CDS             complement(<1..651)
                     /locus_tag="PKJ65_02555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258074.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ40750.1"
                     /translation="MTQGKKDVEQITGRDIDFAKWYTDVVTKAELIDYSQVKGCVVLR
                     PYAFALWENIQKILDDMFKKTGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTLGG
                     SEELTERICVRPTSETLFCDHYKNIIKSWRDLPKKYNQWCSVVRWEKTTRPFLRHREF
                     LWQEGHTIHATVDEAREETHRMLDIYADFCENYLAMPVIKGQKTEKEKFAGAEETYT"
     gene            complement(652..1125)
                     /gene="nrdR"
                     /locus_tag="PKJ65_02560"
     CDS             complement(652..1125)
                     /gene="nrdR"
                     /locus_tag="PKJ65_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNZ40751.1"
                     /translation="MKCPNCGHEEDKVIDSRPSEEGYAIRRRRECLNCKERFTTYENI
                     ERKPIIVVKKDQSRQNFDKAKLLNRLMIACAKRPVSIDKLEDIVNAMELDFHKRHLRE
                     VTSREIGDKVLEYLIDIDYVAYVRFASVYKDFDDMDTFIKEINLVKEKHKKQETE"
     gene            complement(1238..3499)
                     /locus_tag="PKJ65_02565"
     CDS             complement(1238..3499)
                     /locus_tag="PKJ65_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ40752.1"
                     /translation="MSLKSNSIADNLNEEQLKAVTRTQGAVLVLAGAGSGKTRVLTRR
                     IAYILEQGLARPNQILAITFTNKAADQIKQRVYALCEEDKKIYFPWIGTIHSCCLKML
                     RQYPEDAGYRNNFLIYDTDDKKSVIKNCMKQLNISDKAFPVAYVASEISRAKDDLLGY
                     KEYAAANANDFQKKAVAGIYELYQIKMKEYNAMDFDDIILNTYFLLKDNRHILEYFQK
                     IFKYILIDEFQDTNIAQYRIFAMLSVKHKNLFVVGDDDQSIYGFRGANVKNILGFEKD
                     FPDCTVIKLETNYRSYANILTAANAVIKNNVSRKSKTLKTSKPEGSRINVYKAYNELN
                     EAVYVANEIKKLMEKGYSCKDFAVLYRTNAQSRVFERIFRERGLNYRIFGGLSFFSRQ
                     EVKDVLAYMRVVFSTDDDASLFRIINQPKRGIGDTTVKRIQSIAAREYLSSYEIVKNA
                     DRYEQLSSAAEKLKAFSDMIEQLKEYNVPGQLEQLYDNILKLTGIDQYYISEDVNYGR
                     ERVENIKELKSAIVTMNQSQQEDFGELLSLETFLENVTLATDTDKEEESDDYISIMTL
                     HSAKGLEFRVVFLVGMEEGLFPSNLSMSEGAHGLEEERRLCYVGITRAMDILYCIHAE
                     ERTRYGKTEPCEQSRFLKEIPKELINSGKRAFSRREKSGSSFDWFTSSQPVSVTKGGK
                     PVNIPGLKTSTEASVNKQDYSIGDRVMHKKYGEGSIKDLYDDNGMDIVEVNFDMAGVK
                     RMVLEFARLVKIQ"
     gene            3635..>4344
                     /locus_tag="PKJ65_02570"
     CDS             3635..>4344
                     /locus_tag="PKJ65_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40753.1"
                     /translation="MAEQLNTRLRFVRTLTGQEVDRVPFMRVFGGTNHALPSWLEKRP
                     NLSRYMDQLLGFEGAYRGWAIVPANYNLCGLPEDICIEDRGSVRIYRNGIGEIRTWSK
                     DSDYHSGVIEYALKTADDWEKIRPYVVCDVEKRIPRDIDELAEIYKNRDYPLQLTCGG
                     VYGFIRRLAGDENLGYMFYDNPDTVKEIITEYINMLLELWERLCDKIRFDLIESWEDM
                     AYKCGSIISPEHFNEFLAP"
BASE COUNT         1133 a          989 c          790 g         1432 t
ORIGIN      
        1 cgtgtaggtt tcttcggcac ccgcaaactt ttctttctcg gttttctgcc cttttattac
       61 cggcattgcc agataatttt cacagaaatc cgcatatata tccagcatcc gatgagtttc
      121 ttctctggcc tcatcaaccg tagcatgtat ggtatgacct tcctgccaca aaaattctct
      181 gtgtcttaaa aaaggtctgg tagttttttc ccaacgaacc accgaacacc actggttata
      241 tttcttgggc agatctctcc acgattttat aatattcttg taatggtcac agaaaagcgt
      301 ctcggaagta ggtcgtacac atattctttc ggtaagctcc tccgaacctc ccaaagtcac
      361 ccatgccacc tcgggggcaa atccttccac atgatctttt tctttctgta agagcgactc
      421 gggtataaac ataggcatat atacattttc atgacctgtt ttcttaaaca tgtcatccag
      481 tattttctgt atgttttccc ataatgcaaa agcataaggc cgcaacacca cacacccttt
      541 aacctgcgag tagtctatga gttcggcttt tgttactaca tcggtatacc actttgcaaa
      601 atctatatct cttccggtaa tctgctctac atcctttttc ccttgtgtca tttattccgt
      661 ctcctgcttt ttatgttttt ccttaaccag gtttatttct tttataaaag tgtccatatc
      721 gtcaaaatcc ttatacaccg aagcaaaccg tacatatgcc acatagtcta tgtctataag
      781 gtactccagt actttatcgc ctatttctct ggaagtcact tcccttaagt gacgtttatg
      841 aaaatccagt tccatggcat ttacgatatc ctccagtttg tcgatggaaa cgggacgttt
      901 ggcacatgca atcatcagcc tgttgagcag ttttgcttta tcgaaattct gtcttgactg
      961 gtcctttttt actacgatta tgggttttct ttcaatattt tcataagtag taaaacgctc
     1021 tttgcaattt aggcattcac gtctccttct tatggcataa ccctcttccg acggtcttga
     1081 atcgataacc ttgtcttcct catgtccgca atttgggcat ttcattttat gtatatcctt
     1141 tcgtacaaat atctaaaaac tcttaaataa catcttttta aattatattc cataacggta
     1201 ttaatcgcaa tgtacttaag atatgagata ttgagaatca ctgaattttc acaagccttg
     1261 caaattccag caccattctc tttactccgg ccatatcgaa attcacttcc actatatcca
     1321 ttccgttatc gtcatacaaa tcctttatgg aaccttcccc gtatttcttg tgcattaccc
     1381 tgtcgccgat gctgtaatcc tgtttattca cggaagcttc cgtcgaagtt ttcaggcccg
     1441 gaatattcac aggtttcccg ccttttgtca ccgacaccgg ttgcgatgaa gtaaaccaat
     1501 caaaggagga accgctcttt tctcttcttg agaaagcgcg tttaccgctg tttatgagtt
     1561 ccttgggtat ttccttcaga aaacgtgatt gttcgcacgg ttcggtcttg ccgtatctgg
     1621 ttctctcctc ggcatgtatg caatacagta tatccatcgc ccttgttatt cctacataac
     1681 acaaccttcg ctcttcctct aagccgtggg caccctcgct catggacaaa tttgacggaa
     1741 acaacccttc ttccatgcct accagaaata caaccctgaa ctccagtcct tttgccgaat
     1801 gcagagtcat tatggaaata tagtcgtccg attcttcttc cttgtcggta tcggtagcca
     1861 acgtgacatt ttccaaaaaa gtctccaaag acagcagttc tccgaaatct tcctgctgtg
     1921 attgattcat tgtaacaata gcactcttta actcttttat attctccact ctttctctgc
     1981 cgtaattaac atcttccgat atatagtatt gatctattcc ggtaagtttt agaatgttgt
     2041 cgtaaagctg ctccaattgt cccggcacgt tatactcttt caactgctca atcatatcac
     2101 tgaaagcctt aagtttttcc gctgccgaag acagctgctc atacctgtcg gcatttttaa
     2161 caatctcata ggaagaaaga tattcacggg cggctattga ctgtattctt tttacggtgg
     2221 tatcgccgat acctcttttg ggctgattta ttattctaaa cagcgacgcg tcatcgtctg
     2281 tgctgaatac cactctcata tatgcgagca catcttttac ttcctgtctg gaaaagaagc
     2341 tcaaacctcc gaatatcctg tagtttaaac ccctttcgcg aaaaatcctt tcaaataccc
     2401 gggactgggc atttgtcctg tacagtaccg cgaagtcttt acaggaatat cccttttcca
     2461 taagtttttt tatttcattt gcaacatata cggcttcatt cagctcatta tatgccttat
     2521 acacgtttat cctgctgccc tcaggtttgc ttgtcttaag ggttttactc tttctcgaga
     2581 cattgttttt aataacggca ttggcggctg tcaatatgtt tgcgtaagaa cggtaattgg
     2641 tttccagctt tataaccgta caatcgggaa aatctttttc aaaacccagt atattcttta
     2701 cattggctcc tctgaaaccg taaatggact ggtcgtcatc acctaccaca aaaagattct
     2761 tatgtttcac ggaaagcatt gcaaaaatcc tgtattgggc tatgttggtg tcctgaaatt
     2821 catctatcag tatatatttg aaaattttct ggaagtactc cagtatatgc ctgttatctt
     2881 taagaagaaa atatgtattg agtattatgt catcgaaatc catggcattg tactctttca
     2941 tcttaatctg ataaagttca tatatcccgg caaccgcttt tttctggaaa tcgtttgcgt
     3001 ttgcagccgc atattcttta tatcctaaaa gatcatcctt ggctctgctt atctccgaag
     3061 cgacataagc gacaggaaaa gctttatcgg agatattgag ctgtttcatg cagtttttaa
     3121 taacactctt tttatcgtcg gtatcgtata tgagaaagtt gtttctgtag cccgcatctt
     3181 cgggatattg ccttaacatt tttaagcaac aggaatgaat tgtgcctatc caggggaaat
     3241 atatcttctt atcttcttca cataaagcat ataccctttg cttaatctga tctgcggctt
     3301 tgtttgtaaa ggtaatggcc aatatctgat tcggacgggc aagaccctgc tcgagaatat
     3361 atgctatcct tcttgtcagc actcttgtct ttccggaacc cgctcccgcc agcaccagaa
     3421 ccgccccttg tgtacgggta accgctttaa gttgttcttc attcagatta tccgctatac
     3481 tgttgctttt taatgacatt acaaacctct attactaatc gtccaggata ttttaccatt
     3541 aataagtctc tattgaaaga aacaatatgt gttgaagtgt tatatccggt atgataccat
     3601 tcatttattg taatcaatct atgtgaggtt tgatatggca gaacagttaa acacacgtct
     3661 tcgctttgtc aggactctta ccggtcagga agtggacagg gtccctttta tgagggtctt
     3721 cggcgggaca aaccatgcgc ttccttcgtg gctggaaaaa cgacctaacc tttcaagata
     3781 tatggatcaa cttttagggt ttgaaggggc atacagaggc tgggcaatag ttccggcaaa
     3841 ctacaatctc tgcgggcttc ctgaggatat atgtattgaa gacaggggtt cggtacggat
     3901 ttatagaaac ggtataggtg aaataaggac atggtctaaa gacagtgatt atcatagcgg
     3961 agttatagag tacgctttga aaaccgcaga tgactgggaa aagataagac cgtatgtcgt
     4021 atgtgatgtg gaaaaaagaa tacctcggga tatagatgaa cttgccgaaa tttacaaaaa
     4081 cagagattat ccgcttcagc ttacctgcgg aggtgtttac ggatttatcc gaaggcttgc
     4141 cggcgatgag aatctcggat atatgtttta cgacaacccc gatacggtaa aagaaattat
     4201 taccgaatat attaatatgc tgctggaact gtgggaaagg ctttgcgaca aaatacgatt
     4261 cgatcttata gaaagctggg aggacatggc ctacaaatgc ggttccatta tatctcccga
     4321 acacttcaac gagtttttgg cgcc
//
LOCUS       DAOPWC010000078         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20504_length_4320_cov_2.886518, whole genome shotgun sequence.
ACCESSION   DAOPWC010000078 DAOPWC010000000
VERSION     DAOPWC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20504_length_4320_cov_2.886518"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PKJ65_02575"
     CDS             <1..122
                     /locus_tag="PKJ65_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40754.1"
                     /translation="KEKALVSSRCIPFVQEKLSDRCVCCGKPAKHMVVWAVAY"
     gene            129..2510
                     /gene="priA"
                     /locus_tag="PKJ65_02580"
     CDS             129..2510
                     /gene="priA"
                     /locus_tag="PKJ65_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ40755.1"
                     /translation="MKSVTVAAVILDNTTRTFDKVYNYLVPEDMYMYLETGMRILVPF
                     GRADTKKVAYFLNFVPYTEKLKDIKMKYIAEILDDEAVLSKNMFILAKQIRDQYFCTY
                     GQVLSAMVPLGLKVSVEKLILQEDGTLTLFNTYLHSLEGGIKELNRLLKEDRIKIVEQ
                     GVRNIKKKFEKAARLKITSEQVANLIYGDVIKNERHLRVLDLLSTYEDITVNDFSCYE
                     NISRNVLSTLVKKGYIEFFDKPVKPDIVSEEIDEIQPSDKIPTAEQAYVTDTVSLSIE
                     KGIYDKYLIHGVTGSGKTEVYLNLAEKVVNKGKDVIVLVPEIALTPMMVRRFTQRFGN
                     NIEVMHSRLSMKERYNAWMKIKRGEVKIALGTRSCIFAPFNNLGLIAIDEEHDSSYIS
                     DRTPRYHTDFVADKRAQMEKAVLVLGSATPKITTYKAFEKSGKVLTMTNRASGKLPEV
                     TLVDMREDAINGKLSVFSATLLNEIKKNLDNKEQTILFLNRRGYSSIQLCTSCKAVVK
                     CQHCDVTLTYHKGKNNLICHYCGHMQKKSSICPECNEATMMEYGTGTEKCEEQLHELF
                     PEATIIRMDQDTTGMKNSHKEILERFEKEKIDILIGTQMIAKGHDYPLVTLAGILNAD
                     ALGCGAFYESNENAYQLITQSSGRAGRADLEGRAIIQAYNVDSPLITSAIRQDYKSFY
                     LSELKVRKVLSLPPYTNMAFLIFSSSERHLSEEYADIAYRLLSKTSMTVYAPSKAGIA
                     KLHDKIRTRVIVKHKDTKEFMENITKAYLFMIKKLPGKVTMSIDVFKDEFDLL"
     gene            2575..4239
                     /locus_tag="PKJ65_02585"
     CDS             2575..4239
                     /locus_tag="PKJ65_02585"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNZ40756.1"
                     /translation="MLTDIEIAQNAAIKPITKIAKSIGIKEESLELYGPYKAKVKSSA
                     LAGKEQDGKLILVTAITPTPAGEGKTTTSIGLADALRKIGKKSMLALREPSLGPVFGV
                     KGGAAGGGYAQVIPMEDINLHFTGDIHAITAANNLLAALVDNHIYQGNKLKIDPRLVT
                     WKRCLDMNDRQLRYTIDGLGGKSNGMPRQDGFDISVASEIMAILCLSDSIEDLKERLG
                     RIIIGYDFEGEPVTASMLNAQGALAVLLKDAIKPNLVQTLEGTPAFVHGGPFANIAHG
                     CNSIMATKYALRLSDYLVTEAGFGADLGAEKFCDIKCRITGLKPDAVVIVATVKALKY
                     HGGVNKKELGIPNAEAVEKGMPNLYKHIENITEVFKVPAVVAINKFSKDTQEEIDVIK
                     KGCASVKVKAVECLVWEQGGEGGIELAKEVVRLCRKRSRFEYCYDSDLSITEKIKAVA
                     TKIYGADGVEFTSEAKAVLKIINSKGYNNLPVCIAKTQFSFSDNVKLINRPENFTITV
                     RDLRISAGAGFIVALTGEIMTMPGLPKVPAAEAIDIDDEGKITGLF"
BASE COUNT         1431 a          808 c         1021 g         1060 t
ORIGIN      
        1 ttaaagaaaa agctttagtc tcttccagat gtataccttt tgtgcaggaa aaactgtcag
       61 accgctgtgt atgctgcggt aagcctgcaa aacacatggt tgtctgggct gtggcatatt
      121 aaaatctgat gaaaagtgta acggtagctg ccgtaatact ggataataca acaagaacat
      181 ttgacaaggt atataactat cttgtacctg aagatatgta catgtatctt gagaccggta
      241 tgcgcatttt ggtgcctttc ggaagagccg acaccaagaa ggtcgcatat tttctgaact
      301 tcgtaccgta taccgaaaaa ctgaaagaca taaaaatgaa gtacatagcg gaaatattag
      361 acgatgaagc ggtactttcc aagaacatgt ttatacttgc caaacagata agagaccaat
      421 atttctgcac ttacggacag gtattaagtg ctatggttcc tttgggccta aaggtatcgg
      481 tggaaaaact tattcttcag gaagacggaa cgcttaccct gtttaataca tatctgcatt
      541 cgctggaggg cggtataaag gaacttaaca ggttacttaa agaagacaga attaagattg
      601 tcgagcaggg tgtacgcaac atcaaaaaga agtttgagaa agcggcaagg cttaagataa
      661 cttccgagca ggtggcgaat ctcatatacg gggatgttat aaaaaatgaa agacacttaa
      721 gggtactgga tttattatca acttatgaag atataacggt caatgatttt tcctgctatg
      781 agaacatttc ccgaaacgtt ctgtcgacat tggtaaaaaa gggatacata gagtttttcg
      841 ataaacccgt aaaacctgat attgtttccg aagaaattga cgaaatacag ccgagtgata
      901 aaatacctac cgccgagcaa gcatatgtta ccgataccgt ttctttgtcg atagaaaagg
      961 gcatatatga caaatacctt atacacggcg taacgggcag cggcaaaacc gaagtatatc
     1021 tgaatctggc cgagaaagtt gtaaacaaag gtaaggatgt tattgtattg gttccggaaa
     1081 tagcccttac acccatgatg gtcagacgtt tcacacagcg gttcggaaac aatattgaag
     1141 ttatgcacag tcgtctatcc atgaaagagc gatataatgc gtggatgaag ataaaaaggg
     1201 gtgaggtcaa aatagctttg ggaaccagaa gctgtatatt tgcacctttt aacaacctcg
     1261 gccttattgc tatagacgag gagcatgaca gttcctatat atccgacaga acgcccaggt
     1321 atcataccga ttttgttgca gataaacggg cgcagatgga aaaggccgtt cttgtattag
     1381 gttcggcgac acctaagatt actacataca aagcgtttga aaagtcggga aaagtgctta
     1441 ccatgaccaa cagggcatcg ggtaagttgc cggaagtcac gcttgtggat atgcgtgaag
     1501 atgccataaa cggaaagctg tcggtgttca gtgcaacctt actgaatgaa ataaagaaaa
     1561 atctggacaa caaagagcag accatactgt ttctcaacag acggggatat tcctcaatac
     1621 agctatgcac atcctgcaaa gccgttgtga aatgtcagca ttgcgatgtt accttaacat
     1681 accataaagg gaaaaataac ctcatatgcc actattgcgg acacatgcaa aagaagagta
     1741 gcatatgtcc cgaatgtaac gaggcaacca tgatggaata cggtaccggt acggaaaaat
     1801 gcgaagaaca gctgcatgaa ttgtttcccg aagcgaccat aatcaggatg gatcaggata
     1861 ccaccggtat gaaaaactcc cataaggaaa tattggagcg ttttgagaag gaaaagatag
     1921 atattctgat aggcactcag atgatagcaa agggtcatga ttacccgctg gtaacgctgg
     1981 cgggaatctt aaatgccgat gctctgggat gcggagcctt ttacgaatcc aatgaaaatg
     2041 cctaccagct cataactcag tcgtcgggca gagcggggag agccgacctg gaaggaaggg
     2101 cgataattca ggcatataac gtggactcgc ctttaataac cagtgctatc aggcaggatt
     2161 acaagtcctt ttacttgtcg gaacttaaag ttcgtaaagt tttgtccctt cccccttata
     2221 ccaatatggc tttcttaatc ttttcttcat ccgagagaca tttaagtgaa gaatatgccg
     2281 atatagccta cagactgtta agcaaaacgt ccatgacggt atatgctcct tccaaagccg
     2341 gaattgcaaa gctgcatgat aagataagaa ccagagttat agtcaaacac aaagacacaa
     2401 aagagtttat ggagaatata accaaggcat atctgttcat gattaaaaaa cttcccggta
     2461 aagtgaccat gagcattgat gtttttaagg atgaatttga tcttttataa cattcaaact
     2521 cggtttgatg tataataaat cagtatgaat aattaaagaa aaggaaggtg tcatatgctg
     2581 acagatatcg aaattgctca aaatgcggca ataaaaccca ttactaaaat tgcaaagtcc
     2641 ataggaatca aagaagaaag cttggagttg tacggaccgt ataaggcgaa agtgaaaagt
     2701 tccgctttag ccggaaaaga gcaagacggt aaacttatac ttgtcacggc cattacgccc
     2761 acacctgccg gtgagggaaa aaccacaacc agtatagggc ttgccgacgc attaaggaaa
     2821 atagggaaaa agtccatgct ggctttaaga gaaccttctt taggtcccgt attcggagta
     2881 aaaggaggtg ccgcaggggg aggatatgct caggttatcc ccatggaaga tattaatctt
     2941 cattttaccg gtgacattca tgcaataacc gccgcaaaca atcttctggc ggcactggtt
     3001 gataatcata tttatcaggg aaataaactt aaaatagacc cgagacttgt aacctggaaa
     3061 agatgtctgg atatgaatga cagacagttg agatatacaa ttgacggtct cggaggcaaa
     3121 tccaacggaa tgcccagaca ggacggtttt gatatatcgg tagcaagcga gattatggcg
     3181 atactttgct taagcgacag tatagaagat ttaaaagagc ggttaggcag aattataatc
     3241 ggatatgatt ttgaaggaga accggtaacg gcatcaatgc tcaatgcaca aggggctctt
     3301 gcggtgcttc ttaaagatgc aataaaacct aatcttgtac agactcttga aggcacaccg
     3361 gcattcgtgc acggaggacc ttttgccaat atagcccacg gctgtaacag tatcatggcc
     3421 actaagtatg cattgagatt aagcgactat ctggtaaccg aagcgggatt cggtgcggat
     3481 ttaggtgcgg aaaagttctg tgacattaaa tgcagaatta ccggactgaa acccgatgcg
     3541 gtggttattg tggctacggt aaaagctttg aagtatcacg gcggcgtaaa caaaaaagaa
     3601 ttgggaattc ccaatgcgga agcggtggaa aaaggtatgc ccaacctata caaacacatt
     3661 gagaatataa ccgaagtgtt caaggttccc gcagtagtgg caattaataa gttctccaaa
     3721 gacactcagg aagaaattga tgtcataaaa aaaggatgtg catcggttaa agtaaaagcg
     3781 gtagaatgtc tggtatggga gcaaggcgga gaaggcggaa tagaactggc aaaagaagtg
     3841 gtaagacttt gcagaaagcg gagcaggttt gagtactgct atgacagtga cctttccatc
     3901 accgaaaaga ttaaagccgt tgccacaaag atatacggag cggacggtgt ggaatttaca
     3961 tcggaagcca aggcggtgct taagataatc aacagtaaag gctacaacaa ccttccggta
     4021 tgtatagcaa aaacgcaatt ttccttttcc gacaatgtaa aactgatcaa cagacctgag
     4081 aactttacta tcaccgtgcg tgatttacgt atatcggcgg gagcgggatt tatcgtagct
     4141 cttacgggag agataatgac aatgccggga ctgcccaagg tgcctgcagc cgaagccata
     4201 gatattgatg atgagggtaa aataacggga ttgttctgat aagagacaag taatatcttc
     4261 aaattccatt tcctgctgaa gttattacat caactgtgct attatatgct taaggggggg
//
LOCUS       DAOPWC010000079         4285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20713_length_4285_cov_3.633097, whole genome shotgun sequence.
ACCESSION   DAOPWC010000079 DAOPWC010000000
VERSION     DAOPWC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20713_length_4285_cov_3.633097"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKJ65_02590"
     CDS             complement(<1..440)
                     /locus_tag="PKJ65_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40757.1"
                     /translation="MYDISVKKNIEWKAFWLHDPIFKKQPVIQPVDAKRSKYLRTEVQ
                     NRHTLFRKEFEIKNIPIKSAKLYITADDCYKAYINGKFLAYGPAQALAFAYNYNAFEI
                     KKLLKPGLNTLSFHVFYQGMLNMALISADNLNGMICMLEIEYKGG"
     gene            553..1176
                     /locus_tag="PKJ65_02595"
     CDS             553..1176
                     /locus_tag="PKJ65_02595"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNZ40758.1"
                     /translation="MLIHYLLLISASVMLSKLSVRPLRALSYTDTPLFYDDMGCMLSV
                     LLSVSFTVSFYVKTDIISKILMVILLFCLCCITYTDSQAGYIYDRFHIVILLIGIAQL
                     FIKGFSITDIQDSISGCIIGGGFLQLMNCLSILIFKKEGIGGGDVKLCFACGLVLGLK
                     GMISAFIFAFFPAALYAFISGAVRGREVALGPFLCVSIAAVYCIGML"
     gene            1203..1787
                     /gene="loaP"
                     /locus_tag="PKJ65_02600"
     CDS             1203..1787
                     /gene="loaP"
                     /locus_tag="PKJ65_02600"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HNZ40759.1"
                     /translation="MTLGYENVISSRQGYINSWYVFHVKSGEEIKARDNLIKICERKG
                     IKLDFVIPTAIKFIKKKRQRIKSYKTVFPGYVFANGVIDVEIYNTLKGIPGVYRLLRD
                     RSTFALLPVPAEEMELLAGIMNVHGVIEAPEVSFKEGQSAVITKGPLTSFKGKIVAVD
                     KHKNKLTLLVPFLGEERRVEIGFLFAESLDKENS"
     gene            1787..2365
                     /gene="yfbR"
                     /locus_tag="PKJ65_02605"
     CDS             1787..2365
                     /gene="yfbR"
                     /locus_tag="PKJ65_02605"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: protein motif:HMM:NF003009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HNZ40760.1"
                     /translation="MKKGSFFAFIHRIGYINRWGLMRNTSYENLKEHSYDVAVIAQGL
                     ALIGNAKFNKNYDVNRITAAAMFHDVNEALTGDLPTPVKYANKEMEKAYKKIERDAVN
                     NILKMQDDSIRESYEQYFNPSEEDRLVIKAADKISGFIKCKEEKDRGNKDFDKAYDSL
                     KKSIEETDLPEVKYFVENMLPHYNLVLDEFLE"
     gene            2519..2953
                     /gene="rplM"
                     /locus_tag="PKJ65_02610"
     CDS             2519..2953
                     /gene="rplM"
                     /locus_tag="PKJ65_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNZ40761.1"
                     /translation="MKTYMAKADSMEKKWYVVDAEGRTLGRLASEIAKILIGKNKPEY
                     TPSVDTGDYVIVINADKVVVTGKKETQKLYRRHTGYVGGLREVVYRDLMAKHPDRIIT
                     YAVKGMLPKNKLGRHMLKKMKVYAGSEHNHQAQNPIVLDLNE"
     gene            2968..3354
                     /gene="rpsI"
                     /locus_tag="PKJ65_02615"
     CDS             2968..3354
                     /gene="rpsI"
                     /locus_tag="PKJ65_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNZ40762.1"
                     /translation="MTQYYGTGRRKSSVARVRLVTGTGKITINDRDIDDYFGLETLSI
                     IVNQPLEATKLRGKYDVLVNVKGGGFTGQAGAIRQGIARALLEANPDLRAELKKAGYL
                     TRDPREKERKKYGLKKARKASQFSKR"
     gene            3549..>4285
                     /locus_tag="PKJ65_02620"
     CDS             3549..>4285
                     /locus_tag="PKJ65_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40763.1"
                     /translation="MERFILEKLLDWKNSPYRKPLILKGVRQVGKTWVLKEFGKRYYE
                     NTAYFNFDENLEYKQFFETTKDVDRILQNLMIVSGQTIVPEKTLIIFDEVQDCPNVIN
                     SMKYFCENAPQYHVACAGSLLGIALAKPSSFPVGKVNFMQINPMTFEEFLLANGDENL
                     LAFLRSCDSIEPIPDAFFNPLYEKLKMYYITGGMPESVLMWTEARDVSAMQEALSDII
                     GAYERDFAKYPDISEFPKISMVWKSVPS"
BASE COUNT         1380 a          699 c          977 g         1229 t
ORIGIN      
        1 ccgcctttat attctatctc caacatgcat atcatgccgt ttaagttatc cgcagagata
       61 agcgccatgt taagcatacc ctggtagaaa acatggaaag aaagagtatt aagaccgggc
      121 ttcaaaagtt tctttatctc aaaagcgtta taattatacg caaatgccag cgcctgagcg
      181 ggaccgtatg ccaaaaactt accgttgata taggctttgt agcagtcatc tgctgtgata
      241 tatagttttg cactttttat cggaatattc tttatttcaa attctttacg aaacagtgta
      301 tgtctgtttt gcacttccgt tcttaagtac ttggatcttt ttgcatcgac aggctgtata
      361 acaggctgct ttttgaatat gggatcatga agccagaaag ctttccattc aatattcttc
      421 tttaccgata tatcatacat tacaattcac ctccgctata ttgtaacatt ttattcagag
      481 gtttttgcat taatacaccg tttgttcagt ttgtctgtgt attaatacag ttaaagaata
      541 aataatatat taatgttaat acattatctt ctgttaatat ccgcatcggt aatgctgtcc
      601 aagctgtcgg taagaccgtt aagagcattg tcctataccg atactcctct gttttacgat
      661 gatatgggat gcatgttatc ggttttgcta tccgtttctt ttacagtatc tttttatgta
      721 aaaacggata ttataagcaa gatattgatg gttatattgc tcttctgctt atgttgtata
      781 acctataccg actcacaagc cggctacatt tatgacagat ttcacatagt catattgctt
      841 ataggtatag cacaactgtt tataaaaggg ttttcaatca ctgatattca ggacagcata
      901 tcaggttgca ttataggggg aggcttttta caactgatga actgtctgtc aatcttgata
      961 tttaaaaaag aaggaatagg gggtggtgat gtaaagctgt gctttgcatg cggattggta
     1021 ttaggactta aaggaatgat atctgccttt attttcgcct ttttccctgc ggcgctatat
     1081 gcctttatat caggtgcagt acgaggtagg gaggtggcgt taggcccttt cttatgtgtc
     1141 tctatagctg ccgtatattg tattggtatg ctataaatgg tacaatctta aagaggtatt
     1201 gaatgacgtt agggtatgaa aatgtaataa gcagccgaca gggatatatt aattcgtggt
     1261 atgtgtttca tgtaaaaagc ggcgaagaga taaaggcgcg ggataacctt ataaaaatat
     1321 gtgaaagaaa aggtatcaag ctggacttcg ttattccgac ggcaattaaa tttattaaaa
     1381 agaaaagaca aaggataaaa tcctacaaga cggtatttcc gggatatgta tttgcaaacg
     1441 gggtaatcga tgtagagata tacaacacgc tgaagggtat acccggtgtt tacaggttat
     1501 taagggacag aagcactttt gcgctgctgc cggtacccgc cgaggagatg gagttacttg
     1561 cgggtataat gaatgtacac ggagtgatag aggctccgga agtgagtttt aaagagggac
     1621 agagtgcggt tattacaaag gggccgctga caagttttaa aggtaaaatt gttgctgttg
     1681 acaagcacaa gaataagctt accctgctgg ttcctttcct gggagaggag agaagggtgg
     1741 aaataggatt tctgtttgca gaatcgttag ataaggagaa cagttaatga aaaagggcag
     1801 ttttttcgct ttcatacaca gaataggata tataaacaga tgggggctga tgcgtaatac
     1861 cagttatgag aacttaaaag aacacagcta tgatgttgct gtcattgcac agggacttgc
     1921 ccttataggc aatgctaagt tcaacaagaa ctatgatgtt aacagaatta ccgctgcagc
     1981 catgtttcat gatgtaaacg aggcgcttac cggcgatctt cccacacccg taaagtatgc
     2041 aaataaagaa atggagaagg cctacaaaaa gatagaaaga gatgcggtga ataatattct
     2101 taaaatgcag gacgacagta taagagaaag ttatgaacag tattttaacc cttccgagga
     2161 agacaggttg gttataaaag cggcggacaa gatatcggga tttataaaat gcaaagaaga
     2221 aaaagacaga ggaaacaagg atttcgataa ggcatatgat tctttaaaga agagcataga
     2281 agagaccgat cttccggagg taaagtattt tgtggagaat atgcttcccc actataacct
     2341 ggtactggat gaatttctgg aatgaagagc gagtaaaacg acgattgcga gagtatttga
     2401 gagcataaaa aagatttcga gagatatata aaaaacatgt tgacaatacc tgatatatgt
     2461 attaaaatga ctcgtgccgt gtaaaacggc attaaatgtg gaggagaata agtataatat
     2521 gaagacttat atggcaaaag ccgacagtat ggagaagaaa tggtatgtcg tagatgccga
     2581 aggccggact ctgggcagat tggcaagcga gatagccaag attcttatag gaaagaacaa
     2641 gccggagtac acaccgtcag tggatacggg agattatgta atcgtcataa atgccgataa
     2701 ggtagttgta acaggtaaga aggaaacaca gaagttgtac agaagacaca ccggttatgt
     2761 cggcggatta agagaagttg tatatagaga tttgatggct aagcatcctg acagaattat
     2821 cacttatgcc gttaaaggaa tgcttcctaa gaacaaattg ggaagacata tgttaaaaaa
     2881 gatgaaagta tatgcgggaa gcgaacacaa tcatcaggca cagaacccga tagttctgga
     2941 tttgaatgag tagcgggagg ttaagttatg acacagtatt acggaacagg tagaagaaag
     3001 agttctgtcg ccagagtaag attggttacc ggaacaggaa aaataacgat aaacgataga
     3061 gatatagatg attatttcgg attggaaaca ctttcaatca tagtaaatca accgctggaa
     3121 gcaacaaagc tcagaggcaa atatgatgtt ctcgttaatg taaagggtgg cggattcaca
     3181 ggccaggcgg gagctatcag acagggtatt gcaagagcat tactggaagc taatcccgat
     3241 ttaagagcgg aacttaagaa agccggttat cttacaagag atcccagaga aaaagaaaga
     3301 aagaaatacg gtctcaagaa agcaagaaaa gcttcacagt tctccaagag ataatattta
     3361 tcgaaacaat atattacaaa cagcccggga catactccgg gttttttgta tactgttttc
     3421 agttttactg cattttagct cgcaaatata tctgcttttg aaaagagatt ggtttttggc
     3481 tatgaaatat aataaaagtg tgataatata cacataattc tatataataa tgtgattgga
     3541 ggggtaatat ggaacgattt atattggaaa agctattaga ctggaagaat tccccttatc
     3601 gtaaacccct tatactaaaa ggtgtgcgtc aagtcggtaa gacatgggtt ttaaaggagt
     3661 tcggcaagcg ttattatgaa aataccgcct actttaactt tgatgaaaac ctggaatata
     3721 agcagttctt tgagactaca aaggatgtag accgtatttt gcaaaatctg atgatagtaa
     3781 gcggtcaaac tattgtacct gagaagacgc tgattatttt tgatgaggtt caggactgtc
     3841 cgaatgtaat aaactccatg aagtatttct gtgaaaatgc tccgcagtat catgttgcat
     3901 gtgccggttc tctattaggt attgcgcttg caaaaccgtc ttcttttcct gtcggcaagg
     3961 tcaatttcat gcagattaat cccatgacat ttgaggaatt tttgcttgca aacggcgatg
     4021 agaacttact tgcttttttg agaagttgtg actccattga accgattccc gatgcttttt
     4081 ttaatccttt gtacgagaaa ttaaaaatgt actatattac cggcggtatg cctgagtccg
     4141 tgttaatgtg gacagaggca cgggatgttt ccgctatgca ggaagccttg tccgatatta
     4201 tcggtgccta tgagcgtgat tttgcaaagt accccgacat aagcgaattc cctaaaatct
     4261 ccatggtctg gaaatccgtt ccctc
//
LOCUS       DAOPWC010000080         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20825_length_4271_cov_3.379032, whole genome shotgun sequence.
ACCESSION   DAOPWC010000080 DAOPWC010000000
VERSION     DAOPWC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20825_length_4271_cov_3.379032"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKJ65_02625"
     CDS             complement(<1..566)
                     /locus_tag="PKJ65_02625"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ40764.1"
                     /translation="MSITIKDLTVNYLTNPLGIDEIPRFSWKLAQDGRGNKQRSYRIK
                     VMTGGKVVWDSKNVRSDESVLVEYKGKKLLPRTTYRWNVSVTDKKGERAQSEDAFFDT
                     GKLEEEWVGKYIQAGYRVPRELAASSPYLRKRFDIPDDVSKAMLYITGAGFFEPYING
                     KKINDHVMDPPFTAYDRTLLYYTFNVTE"
     gene            complement(754..1959)
                     /gene="tuf"
                     /locus_tag="PKJ65_02630"
     CDS             complement(754..1959)
                     /gene="tuf"
                     /locus_tag="PKJ65_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNZ40765.1"
                     /translation="MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLALADKNIV
                     FKAYEQIDAAPEEKARGITIATAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTKEHILLARQVGVPYIVVYMNKTDQVDDPEILELVEMELREILN
                     EYEFPGDDIPIVMGSALQALQSTSTDQSAPEYASILKLMETVDSYIPTPQRDVDKPFL
                     MPVEDVFSITGRGTVATGRVERGTVKVQDTVEIVGLTEKISSTVVTGVEMFRKILDFA
                     QAGDNVGCLLRGVQRTDIERGQVLAKPGSIKPHTDFVGQVYVLTAEEGGRHTPFFTGY
                     RPQFYFRTTDVTGVANLPEGVEMVMPGDHIDMRIALITPIAMDEGLRFAIREGGKTVG
                     AGSVVKIMK"
     gene            complement(2037..4112)
                     /gene="fusA"
                     /locus_tag="PKJ65_02635"
     CDS             complement(2037..4112)
                     /gene="fusA"
                     /locus_tag="PKJ65_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ40766.1"
                     /translation="MPRDYALEFTRNIGIMAHIDAGKTTTTERILYYTGKLHRMGEVH
                     EGAATMDWMEQEQERGITITSAATTAYWKDYRLNIIDTPGHVDFTAEVERSLRVLDGA
                     VSVFCAKGGVEPQSETVWRQADRYKVPRIAYINKMDTTGADFFRCVEMMRKRLNANPV
                     PIQLPIGKESDFTGIIDLVKFKAYFYRDDLGNSIEELDIPDDMLDKAAEYRIKLIESV
                     CDESECLMEKYLEGEDVEEDEIKAALRAGTVNLSITPVLCGSSYKNKGVQKLLDAITD
                     YLPSPVDVCDIEGFSMHDHSKVITKPSRDDEPFAALAFKIMSDPFVGKICFFRVYSGT
                     LETGSYVLNATKDKKERIGRILMMHSNSRTDISKVYSGDIACAVGLKFTVTGDTLCDP
                     KDPVILESMQFPEPVISIAVEGKDKVHQDRMALALQKLSEEDPTFKFKTDKDTGQVLI
                     SGMGELHLDIIVDRMRREFSVEATKGKPQVTYKETITKTSRAEGKFIRQTGGHGQYGH
                     VVLEVAPAKPGEGFTFIDKTVGGVIPKIYIPAVEKGIRESLSNGIQAGYPVVDVTVTL
                     LDGSFHEVDSSDMAFMIAANMGFREACMNADPILLEPVMRVDIFVPENCLGDVLGDIT
                     AKRGKIENVEAGEIESVITATVPLKEMFGYATDLRSNTQGRGTFTMQYHHFERIPDDL
                     AAKILKTNY"
     gene            complement(4128..>4271)
                     /locus_tag="PKJ65_02640"
     CDS             complement(4128..>4271)
                     /locus_tag="PKJ65_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523252.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNZ40767.1"
                     /translation="NRNEHTMKQRLAAEIMDSVNGVSNTFKRKEEMHRMAEANKAFAH
                     YRW"
BASE COUNT         1039 a         1045 c          838 g         1349 t
ORIGIN      
        1 aactcggtta cattgaaagt gtagtacaga agagttctgt catatgcggt gaaaggcgga
       61 tccattacat ggtcatttat ctttttaccg ttgatatagg gctcaaagaa tcccgccccg
      121 gttatgtaca gcattgcctt ggaaacatca tcggggatat cgaatctttt tcttaagtaa
      181 ggagaagaag ctgcaagttc tctgggtact ctgtaccctg cctgtatata tttgccgacc
      241 cattcttctt ccagtttgcc ggtatcaaaa aaagcatctt cactttgtgc tctttcacct
      301 tttttgtcgg ttacactgac attccagcga tatgtggtcc tcggcagcag tttcttgccc
      361 ttatactcga caagtacgga ttcatcgctt cggacatttt tggaatccca taccaccttg
      421 ccgcccgtca tgacctttat tctgtatgaa cgttgtttgt tacctcttcc gtcctgtgca
      481 agcttccatg agaatctcgg aatctcatca attcccaacg ggttggtaag atagtttact
      541 gttaaatcct tgatagtgat agacatttac tcatctcctg acttaattat tttgcctttt
      601 aattatatct tttttataac tgcatgacaa ggtagcaaaa gtcccggtaa actcaaaaga
      661 aaataaaaaa gaacacattt tgattcaaaa ttgctttaaa aaaacggaag caagtgtaaa
      721 cctgcttccg ctgagataac tattaaaata gtcttacttc attatcttaa caacgcttcc
      781 tgcgcctacg gtctttccgc cttctcttat agcgaatctc aagccttcgt ccatagcgat
      841 aggtgtgata agagcaattc tcatatcaat gtggtcgccg ggcataacca tttccacgcc
      901 ttcgggaagg ttggcaacac cggtaacgtc ggttgttctg aaatagaact gaggtctgta
      961 acctgtgaag aacggtgtat gtcttccgcc ttcttcagct gtcagtacgt atacctgtcc
     1021 gacaaaatcg gtatgcggct tgattgatcc gggttttgca agaacctgtc ctctttcgat
     1081 atcggttctc tgaactcctc tcaaaagaca tcctacattg tcacctgcct gtgcgaaatc
     1141 cagaatcttt ctgaacattt ccacgcctgt tacaacggta gatgaaatct tttccgtaag
     1201 tcctactatc tctacagtat cctgaacttt aaccgttcct ctttccactc ttccggtagc
     1261 aaccgttcct ctacccgtga tggagaaaac gtcctcaacc ggcattaaga aaggcttatc
     1321 tacatctctc tgcggagtag gaatatatga gtcaacagtt tccataagtt taaggattga
     1381 tgcatactcg ggtgcactct gatcggtaga agttgactgc agagcctgaa gcgccgaacc
     1441 catgactatg ggaatatcat ctccggggaa ctcatactcg ttgagtattt ctcttaattc
     1501 catttctacg agttccagaa tctcgggatc atcaacctgg tcggttttat tcatgtatac
     1561 tacaatgtac ggaacgccaa cctgacgtgc aagcagtata tgctcttttg tctgaggcat
     1621 cggaccgtca gcagcggaaa caacgagtat tgctccgtcc atctgagccg caccggtaat
     1681 catgttctta acatagtctg cgtgaccggg acagtcaaca tgcgcatagt gtctgttctt
     1741 tgtctcatac tcaacgtgag ctgtagcaat ggtgattcct cttgcttttt cttcaggtgc
     1801 tgcgtcaatc tgctcgtatg ccttgaaaac tatgttttta tcagctaaag ccaatgtctt
     1861 tgtaatagca gcagttaatg tggtcttgcc atggtctacg tgtccgatag taccgatgtt
     1921 gcaatgtggt ttagttcttt caaattttgc ttttgccatt ttattttttc ctccaattat
     1981 aatgagctga tttatatatt aatttatata cattctacat atgaatgcgg gttgtgtcaa
     2041 tagttcgttt tgagtatttt tgccgctaaa tcatccggta ttctctcaaa atgatggtat
     2101 tgcatggtaa atgtgcctct tccctgcgta ttggatcgaa ggtcggtagc atatccgaac
     2161 atctctttca agggcactgt tgcggtgatg accgattcaa tttccccggc ctccacattc
     2221 tctatttttc ctctttttgc cgttatatcg cctaaaacat cacccagaca gttttccggg
     2281 acaaatatgt ccacacgcat taccggttcc aaaagaatgg gatcggcatt catacatgct
     2341 tctctgaaac ccatgtttgc ggctatcatg aaagccatat ccgaggagtc cacctcatgg
     2401 aaagagccgt ccagaagggt tacggtaaca tccacaacgg gatatcccgc ctgaataccg
     2461 ttgcttaagg attctcttat gcctttttct accgccggaa tatatatttt gggtattacc
     2521 cctcctacgg ttttatcgat aaaggtaaac ccttcgcccg gtttggccgg tgccacttcc
     2581 agcactacat gaccgtactg tccgtgtcct ccggtctgcc tgatgaattt tccttctgca
     2641 cgcgaagttt tcgtgatggt ttccttgtaa gtcacctgag gttttccttt ggttgcttcc
     2701 acggaaaact cccttctcat cctatctacg ataatatcca gatgaagttc tcccattccc
     2761 gatatgagaa cctgtccggt atctttgtcg gttttgaatt tgaaggtcgg atcttcttcg
     2821 gaaagtttct gcaaagccaa tgccattctg tcctgatgaa ctttatcttt gccttcgaca
     2881 gctatggata taaccggttc gggaaactgc attgactcca aaattacagg gtctttgggg
     2941 tcgcaaagtg tatctcccgt cacagtgaac tttaagccta ccgcacaggc aatatctccg
     3001 gaataaactt tactgatatc ggtcctgctg ttggagtgca tcatgagtat tcttccgata
     3061 cgctcttttt tatccttggt tgcattcaga acataggaac ctgtctccag cgttccggaa
     3121 tatacccgga aaaaacatat ctttccgaca aaaggatcgg acattatttt gaatgccagt
     3181 gcggcaaacg gttcatcatc ccgtgacggt ttggttatca ctttggaatg atcatgcata
     3241 ctgaagcctt ctatgtcaca aacatccacc ggcgacggca gataatcggt tatagcatcc
     3301 agaagtttct gaaccccttt gtttttatat gaagatccgc acaatacggg agttatgctt
     3361 aaattgacgg ttcctgccct caaagctgct tttatttcgt cttcctccac atcctctccc
     3421 tcaagatact tctccataag acattccgat tcatcacata ccgattcgat aagtttaatt
     3481 ctatactcgg ctgctttatc cagcatatca tcaggtatat ccagttcctc aatactattg
     3541 cccaaatcat ccctgtagaa atatgctttg aacttaacaa ggtctattat tcccgtaaag
     3601 tccgactctt tacctatagg taactggatg ggaaccggat tggcattgag tcgctttctc
     3661 atcatttcca cacaacggaa aaagtccgca ccggtagtat ccatcttatt gatataggca
     3721 attctgggta ccttatacct gtccgcctgt ctccataccg tttccgactg gggttccaca
     3781 ccgccttttg cacagaaaac cgaaacggca ccgtcaagca ctctaagact tctctccacc
     3841 tcggcggtaa aatccacatg accgggagta tcgataatgt tcagtcggta gtctttccaa
     3901 taggctgtcg tagcggcgga agttatggta ataccacgct cctgctcctg ctccatccag
     3961 tccattgttg ccgctccctc atgaacctct cccatcctgt gaagctttcc ggtatagtag
     4021 agaattcttt cggtagttgt ggttttgccg gcatcaatat gagccattat tccgatattt
     4081 ctggtaaatt ccagtgcata atctctcggc atattattct ttccgaatta ccacctgtaa
     4141 tgagcaaacg ccttattggc ctctgccatt ctgtgcattt cttcttttct cttgaaggta
     4201 ttgctgactc cgttaacgga atccataatc tctgcggcaa gtcgctgttt catggtgtgt
     4261 tcattgcggt t
//
LOCUS       DAOPWC010000081         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_20895_length_4260_cov_4.037337, whole genome shotgun sequence.
ACCESSION   DAOPWC010000081 DAOPWC010000000
VERSION     DAOPWC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20895_length_4260_cov_4.037337"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKJ65_02645"
     CDS             complement(<1..184)
                     /locus_tag="PKJ65_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40768.1"
                     /translation="MESWYYEKIVYRKKTRKDDLISLAVVSVTLAICIVSYYFLRNLS
                     FIIWLGVIYACYRIITS"
     gene            311..772
                     /gene="ispF"
                     /locus_tag="PKJ65_02650"
     CDS             311..772
                     /gene="ispF"
                     /locus_tag="PKJ65_02650"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNZ40769.1"
                     /translation="MKCAIGQDSHKFSENADKKLILAGVHIKNHPGLIANSDGDVIYH
                     ALTNAISGITSVNILGKTADIMCKGGITDSSLYVKEALKSLEYKIEHVSFTVECLTPV
                     LFPYIEEMKGNIAMLLGIDKKDVGLTATSGEGLSSFGKGSGIMCFCIITVS"
     gene            complement(734..1441)
                     /gene="ispD"
                     /locus_tag="PKJ65_02655"
     CDS             complement(734..1441)
                     /gene="ispD"
                     /locus_tag="PKJ65_02655"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNZ40770.1"
                     /translation="MESVSVVIAAGGKSGRMDGINKLLAPLGGVPVIIRTVKAFHDIR
                     YIKQIILVIPKKDESEYLSLLSEYRVNRKVKIAYAGNTRRHSVYNGLLSLSDKEGIVL
                     IHDGARPLVTRKIIDECVDGAKRYGVCVAASKSTDTVKIADSIMFIEKTLDRDKVYNA
                     QTPQAFKTEFALDFHKRAEADDLEVTDDCMIAEHYGHNVKIIESDSLNLKITTKSDLV
                     IASAILADGYYTEAHDS"
     gene            complement(1445..1954)
                     /locus_tag="PKJ65_02660"
     CDS             complement(1445..1954)
                     /locus_tag="PKJ65_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ40771.1"
                     /translation="MSYSIGDKIVYPMHGAGVIVDKVDRKIMDEIRCYYVVKSPISEM
                     VAMLPVDNMDGIGIRDVITDSEADELLDEIKTMDTEETSNWNKRYRENMEKLKDGSIY
                     EIAQIAKSLMTRDKSLSTGERKLMINAKNAVLSEIMLAKNISLVQAENALKESIYCNS
                     AEDSAVKEE"
     gene            2307..3278
                     /locus_tag="PKJ65_02665"
     CDS             2307..3278
                     /locus_tag="PKJ65_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HNZ40772.1"
                     /translation="MNNTWENKLELFAKNVQIMKSEFVWQNVSIKRMAALLYALEGKT
                     VDLETIRQCHNLIKQNTGAFSTFRGSMALGLATMLSLSGNPENSLSATLKVYKLLKDV
                     KFRASYLLVVAAYRIAIQADASDYENAVMRTRAFFDGMKAKNFFYTGQDDYIFAAMLG
                     ISGLDLAEGTERIKQIYNQLKREFRNKNSVQILAQVLALGEADDSIVNRVLALRDELR
                     TQKIKLDKAYTLPFLGIFALLPVEIETIVYKIGEAQDTLRSKKGFRTLSVSTGELLLF
                     AASIVASDYTENIKDGVLTATLSTCIANIIIAQEAAMVAAASSSSSV"
     gene            complement(3505..4179)
                     /locus_tag="PKJ65_02670"
     CDS             complement(3505..4179)
                     /locus_tag="PKJ65_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40773.1"
                     /translation="MTNTPILQLEKVSYEYRNKYQTVRAVKDFSYEFFKGKFYAITGK
                     SGSGKTTLLSLMVGLDVPAKGVIKYDGTNLKEMNMDKYRRDCISLIYQDFNLFPLLTA
                     MENVMYPLKLKGLKTSECETLAKEKLNMVGMDETFYKRYPSMLSGGEQQRVSIARALA
                     TGSSILLADEPTGNLDTENGDIIVSILKNLAHNNDFCVIVVTHDLEIASVTDVQLNLK
                     DGMLVT"
BASE COUNT         1281 a          883 c          801 g         1295 t
ORIGIN      
        1 tggaagttat aatcctgtag catgcatata tgacacccaa ccagataata aatgagagat
       61 ttcgcagaaa gtaataggaa actatacata tcgcaagcgt aactgaaacg acggcaagcg
      121 atatcaaatc atcttttctg gttttctttc tgtatacaat cttctcatag taccaacttt
      181 ccatatgcat cttcctttca atatgctaaa tatatcatat aaagggtttt tatgtgtgta
      241 ctttttgtta acttttccga tattatggta ttatgttatt caagtattaa acaacgaggt
      301 gacatacatc atgaagtgcg caatcgggca ggacagtcat aagttttccg aaaatgcaga
      361 caagaagctt attcttgcgg gagttcacat aaaaaatcat ccgggactta tcgcaaacag
      421 cgacggggat gttatctatc atgctctgac caatgcgata agcggtatta cctcggtaaa
      481 tattttagga aaaaccgcag atataatgtg caaaggagga ataaccgaca gttctctata
      541 tgtaaaagaa gctttgaaaa gcctggaata taaaatagag catgtcagct ttacggtgga
      601 gtgtttaact ccggttctct ttccttacat agaggaaatg aaaggcaata tagcaatgct
      661 tctcggaata gataaaaaag atgtgggttt gacagccaca tcgggtgaag gtttgtcatc
      721 attcggaaaa ggttcaggaa tcatgtgctt ctgtataata accgtcagct aaaatagccg
      781 aagctattac taaatccgat tttgtagtta tttttaaatt caaagaatcg gactctataa
      841 tctttacatt atgtccgtaa tgctccgcta tcatgcagtc atcggtgact tccagatcat
      901 cggcttccgc tcttttgtga aaatccaaag caaattccgt cttaaaagcc tgcggtgtct
      961 gggcattgta taccttgtct ctgtcaaggg ttttttctat aaacattatg ctgtccgcta
     1021 tctttacggt atcggtgctc tttgaagcgg ccacacatac cccgtatcgc tttgctccgt
     1081 ctacacactc atctataatc ttacgggtaa ccagcggtct ggcaccgtcg tgtataagta
     1141 caataccttc tttgtcagat aatgacagca atccgttata caccgaatgt ctgcgggtgt
     1201 ttcccgcata cgcaatcttt acttttctat tcaccctata ttcagacaat aaagataagt
     1261 actccgactc atctttttta ggtataacaa gtattatctg tttaatgtat cttatatcat
     1321 gaaacgcttt aaccgtccgg ataataacgg gaacaccgcc aagaggcgcc aacagtttgt
     1381 ttattccgtc catcctgccg gatttgccgc ccgcagcaat aaccacactt accgattcca
     1441 tagattattc ttccttaacg gccgaatcct cagccgaatt acaatatata ctttccttta
     1501 aagcgttctc cgcctgaacc agagaaatat tcttagccag cattatttca ctcaatacgg
     1561 catttttcgc attaatcatc aattttctct cacctgtcga gaggcttttg tcacgggtca
     1621 ttaatgattt agcaatctga gcaatttcat atattgatcc gtccttgagt ttctccatat
     1681 tttcacggta tctcttattc cagttggatg tttcttcggt atccattgtc tttatctcat
     1741 ccagaagttc atccgcttcg gaatcggtga taacatccct tatacctata ccgtccatat
     1801 tatccacggg aagcatagcg accatttcac ttatgggact ttttactaca taatagcatc
     1861 ggatttcatc cataatcttt ctgtcgactt tatcaacaat gacgcctgcg ccatgcatag
     1921 gatatactat cttatccccg atactgtaac tcaattaaag atacctcctg cacaatatta
     1981 tactcttttt agtgcctgct gtcaaaagca acacaggcga aaatgagcag gaaaattgaa
     2041 cgtaattttt ttgacaaaac ataaagaatg aacgaatgtt gcctctttaa aacttccctt
     2101 ttaagccatt tgccacatta aagcacatgc gccgtgaaca gcagcagtgt caaaaattat
     2161 taagcgatgc aggatattga gctaagagaa agttgaatgt ataattgagt tgattatcat
     2221 atggcataaa tacaggaaag atgatttgac acaactttat ttaatactta cataacagca
     2281 aattcaattt acggaggtct tttcagatga ataacacatg ggaaaacaag ttggaacttt
     2341 ttgcaaaaaa cgtgcaaatc atgaaatcgg agtttgtctg gcaaaatgtc tcgataaagc
     2401 gtatggcggc gcttctgtac gcactggaag gcaaaacggt tgacttagag acaatcaggc
     2461 agtgtcacaa tttgattaaa cagaacacgg gcgcgttttc cacattccgc gggagcatgg
     2521 cgctgggttt ggcgacaatg ctctctctat ccggtaatcc tgagaattcg ttaagtgcaa
     2581 cccttaaagt atacaagttg ctgaaagatg taaagttcag agcttcctac cttttggtgg
     2641 ttgccgcata ccggattgca atacaagccg atgcttccga ttatgaaaac gcggtaatgc
     2701 gcacgagggc attttttgac ggaatgaaag ctaaaaattt cttttacacc gggcaggatg
     2761 attatatttt tgcggctatg ttgggaattt ccggtttgga tcttgccgaa ggaacggagc
     2821 gcattaagca aatttataat cagcttaaaa gagagttcag aaataaaaac agcgttcaga
     2881 ttctggcaca ggtgctggca ctcggagagg ctgacgacag tattgttaat cgtgttcttg
     2941 ctctgcgtga tgagctcagg acgcaaaaaa tcaaactgga caaagcttat acattaccgt
     3001 ttttgggaat ctttgcactg ctgccggtag aaattgaaac aatcgtttat aaaataggag
     3061 aagcacaaga tactcttagg agcaaaaagg gattcagaac tctgtccgta agtacggggg
     3121 aactacttct gtttgcggca tcaattgtag caagcgacta taccgaaaat ataaaagacg
     3181 gagtgctgac ggcaacgctt tcaacatgta tagccaatat tattatagcg caggaagccg
     3241 ctatggtagc agctgcatcg tcatcttcat cagtttaaca acatgctgaa taagaagcgt
     3301 tcgtgattaa atagcaatca gattacgcag gaaacaggat ataacaatat taaaagaatt
     3361 atgacaatcc tttcttaacg atatgtattc gaagactcaa aggaaacaca aggttatgca
     3421 acggttgcaa aaagcttttc aaccgagttc ggaaaaccgt gaatagtaaa ttgaagtaaa
     3481 tatcagtgga aaaatcaaca ttatctaagt aacaagcata ccgtccttta agttcagctg
     3541 cacatcggta accgaagcaa tttccaaatc atgtgttaca actataacac agaaatcgtt
     3601 gttgtgtgca agattcttta atatggaaac aattatatcg ccgttctccg tatcaaggtt
     3661 acccgtcggc tcatccgcta aaagtattga ggagccggtt gccaaagcac gtgcaatcga
     3721 tactctctgc tgttcgcctc ccgacaacat ggaaggatag cgtttataaa aagtttcatc
     3781 catccctacc atattcagct tttcctttgc aagtgtctcg cactcgcttg ttttcaatcc
     3841 ttttagtttt aaaggataca ttacattctc catcgcggta agcaacggaa acagattgaa
     3901 atcctgatat atcagtgaaa tacaatctct tctgtattta tccatattca tctctttaag
     3961 attggttccg tcgtatttaa taacgccttt tgcgggaaca tccaacccta ccattaatga
     4021 taataaagtg gttttccccg aaccggattt tcctgttatt gcgtagaatt tccccttaaa
     4081 gaattcataa gaaaaatcct ttaccgctct taccgtctga tatttgtttc tgtattcata
     4141 gcttactttt tccaactgca atatcggtgt atttgtcatt ttgtcggtct ccttaatcgt
     4201 tcgctttcat tattttcatt actttaacac ttgctattct taatgcttct atcgccgtac
//
LOCUS       DAOPWC010000082         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21074_length_4235_cov_3.918900, whole genome shotgun sequence.
ACCESSION   DAOPWC010000082 DAOPWC010000000
VERSION     DAOPWC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21074_length_4235_cov_3.918900"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKJ65_02675"
     CDS             complement(<1..564)
                     /locus_tag="PKJ65_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HNZ40774.1"
                     /translation="MIENFDIDNRLIKLADIVQQQCDAQFKRLEDTAETNQVKVTAAF
                     QRNRVSDAYFAETTGYGYSDRGRELLDEVYADIFRTEDALVRESIASGTAAISLAMAS
                     NVRPGDEIIFANGLPYDTLRSTVGITEARGSLAEYGVTHKIIDTKDNKIDYSAVAASL
                     SKKTKMVFVQRSRGYSIKPPVNKEQFET"
     gene            complement(561..1769)
                     /locus_tag="PKJ65_02680"
     CDS             complement(561..1769)
                     /locus_tag="PKJ65_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ40775.1"
                     /translation="MKKTDTYYIHEPGRVFKAIVRGIVRAGFRVVLNLRSEKSDDIDK
                     LYGATILIGNHLSAIDPFVMGAYVTKRWVHYLTSQSYFEYPALKKILTYLGAIPKRQG
                     MSDIRAMKVALNVLKDNKVIGLFPEGARTIDGSMLPFNNSAAKLIKKTGSNVVVSYLC
                     GASISLPRWYRGCSGGFRRGGIEHKAYVLLTKEQVSTMSVEQIDLRIREALYYNEYDW
                     QREKKVEFKSRSMAEGMHAILHKCPKCLTDYSMKSKGMFLYCDECDNTAVVDKYGLLN
                     AQTEDDVSFGDMPEWRAWQNTHLEKEVASDDFVMEFPGSLTVKDSYGKVLKEYTDSYL
                     KITKVEVIYTYPEGKTVLPFHSITDAVCDYSLRFEINQEQFSYQFTPLSGQAVIKITD
                     AIKCIKGILR"
     gene            complement(1868..2713)
                     /locus_tag="PKJ65_02685"
     CDS             complement(1868..2713)
                     /locus_tag="PKJ65_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ40776.1"
                     /translation="MITRNPMQLKAFIKNKATEKHISAQLVMQNYMMERLLERISLSS
                     FKQNFILKGGFLISSIVGLDTRATMDMDMTIKGVALTHETIRSIFMKICATQVDDDIQ
                     FEVSGVSDIRISEDYHGIRVALKANYPPIRVPLTVDITTGDVITPCAIEYTYRLLFDK
                     RTINILSYNIETILAEKLETVLTRNIANTRLRDYYDIYILYNLHSLKYDKATLLNALV
                     RTTKRRGSSMILTEYQHIIKEICKSDALRNFWDKYRNEYDYAKDISFDDVCNTLRIII
                     DAILT"
     gene            complement(2710..3303)
                     /locus_tag="PKJ65_02690"
     CDS             complement(2710..3303)
                     /locus_tag="PKJ65_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiEi antitoxin N-terminal domain-containing
                     protein"
                     /protein_id="HNZ40777.1"
                     /translation="MDKKEKIDELLDTLEDGIITASQVSEAGLHRSVLQNLVESGEIY
                     RYGRGIYVKSNAWEDDLYLLQCKYGRGIYSHDTALYLLGYSDRTPAKYTMTFPKGYNA
                     PSLHKENIIVKRVIPKNYQSGIIEIESLSGNPIRVYDLERTLCDILRGSGSDIQIVGE
                     AMKRYATSKDRNIHKLMQYAEQLRVKPKVLRYMEVLL"
     gene            3568..4230
                     /locus_tag="PKJ65_02695"
     CDS             3568..4230
                     /locus_tag="PKJ65_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ40778.1"
                     /translation="MRYTLCLFDLDGTLTDPKIGITKSFQYALSYFGIHEELDNLTNF
                     IGPPLRESFKNSYGFSDTDTEKAVAKYREYFTATGLYENAVYPKIPETLEKLKNNGCI
                     LAVATSKVTAYAKKILKHFNLDGYFTLVSGDEMDGSLTKNGKHALINIALDRLDPERT
                     LSVVMIGDRKDDIIGANNAGIDSIGVLWGYGTVEELETANATKIVHTSDELYSLIIEE
                     CR"
BASE COUNT         1206 a          921 c          753 g         1355 t
ORIGIN      
        1 tgtttcaaac tgctctttat ttacgggagg tttgatgctg taacctctgg atcgttgaac
       61 aaataccatt tttgtctttt tgctcaagct tgcagccacg gcggaataat caattttatt
      121 atccttcgta tcaattatct tatgggtaac accgtattcc gcaagacttc ctctggcttc
      181 ggttataccc actgtacttc ttaaagtatc atacggcaac ccgttggcaa aaattatttc
      241 atcgcccgga cgaacattgg acgccattgc aagcgatatg gcggcggtac ccgaagcaat
      301 gctttcccgt acaagggcat cttcggtacg gaatatatcg gcatatactt catccagaag
      361 ttccctgcct ctgtcggaat agccgtaacc tgttgtttcg gcaaaatatg catcacttac
      421 cctgtttctc tgaaaagcag ccgttacctt aacctgattt gtttcggcgg tatcctccag
      481 tctcttaaac tgtgcatcgc attgttgctg tacgatatcg gccagcttga taagtctgtt
      541 atctatgtcg aaattttcaa tcatcgtaat atccctttga tgcattttat agcgtcggtt
      601 attttaataa cagcctgccc tgataaggga gtaaactgat aggaaaactg ttcctgattt
      661 atctcaaacc gtaaactgta gtcgcacacg gcatcggtaa tgctgtggaa cggcaacacg
      721 gtttttcctt cgggatatgt gtagataact tcaaccttag tgattttcag ataactgtcc
      781 gtatactctt taagtacctt tccgtaactg tcctttacgg taagcgaacc gggaaattcc
      841 attacaaaat catcggaagc tacttccttt tccaggtgcg tgttttgcca tgctctccat
      901 tcgggcatat caccaaatga tacatcatct tccgtttgtg cattcaacag tccgtattta
      961 tcaaccaccg cggtgttatc gcattcatcg cagtacaaaa acataccttt actcttcatg
     1021 gaataatcgg taagacattt aggacatttg tgtaatatgg catgcattcc ttcggccatg
     1081 gaacggcttt taaattccac ctttttttcc cgctgccaat catattcatt gtaataaaga
     1141 gcttctctta ttcttaaatc aatctgttct accgacatgg tgctcacctg ttcttttgtc
     1201 agcagcacat atgccttatg ttctattccg cctcttctga aacctcccga acatcccctg
     1261 taccatctcg gcaacgaaat agaagctccg caaagatatg aaaccaccac attggaaccg
     1321 gttttcttaa tcagtttagc ggcactgtta ttaaacggaa gcatggagcc gtcaattgtc
     1381 cttgctcctt cgggaaacaa tcctatcacc ttgttgtctt taagcacatt caaagctact
     1441 ttcatagccc ttatatcgct cataccctga cgcttgggta tggctcccag atatgtaagt
     1501 atctttttca atgcgggata ttcgaaatat gattgcgaag taagataatg cacccatctt
     1561 tttgttacat acgcacccat tacaaaaggg tctattgcgg ataaatggtt accaataagt
     1621 atggtagctc cgtaaagttt atcaatatca tcggatttct ctgaacgaag attcaatacc
     1681 acccggaatc ccgcgcgaac aatacctctg actatcgctt taaatactct tcccggttca
     1741 tgtatgtaat aggtgtctgt ttttttcaat cttactccct catcttttgc aaagtgtatt
     1801 ctatcattta cccggcatgt tatcaacatg tcgcgattag agatatttag ataatacttc
     1861 catatgttca tgtcaaaatc gcgtctataa taattcgcaa agtattacag acatcatcaa
     1921 aagatatatc cttagcataa tcatattcat ttctgtactt atcccaaaaa ttcctgagtg
     1981 catcactttt gcagatttct tttattatat gttggtactc tgtcaaaatc atactactgc
     2041 cacgtctctt cgtagttcgc acaagagcat tgagtagcgt tgctttgtca tattttaagc
     2101 tgtgcagatt atacagaata taaatatcgt agtagtctct tagacgagta tttgcaatat
     2161 ttctagttaa cactgtttct aacttttccg ctagtatcgt ttcaatatta taggaaagta
     2221 tattaatcgt tctcttatca aacaataacc gatatgtgta ctctatagca cagggggtta
     2281 tgacatcgcc tgtagtaata tccactgtaa gtggtactct gattggcgga taatttgctt
     2341 tcaatgcaac acgtataccg tgataatctt cacttatgcg gatatcagaa acacctgaaa
     2401 cctcaaactg aatgtcatcg tcaacctgtg tagcacatat tttcatgaag atagagcgaa
     2461 tagtttcatg tgtcagagca actcccttaa tcgtcatatc catgtccatc gtcgctcgcg
     2521 tatccaatcc tactatggat gaaatcaaaa aacccccttt cagaataaaa ttttgtttga
     2581 atgatgagag ggatattcgt tcaagcagac gttccatcat atagttttgc attacaagtt
     2641 gtgcagatat atgtttttct gtcgctttat tcttgataaa ggctttcaat tgcataggat
     2701 ttcttgtaat cacaacaaaa cctccatata acgaagaact ttcggcttca cgcgaagctg
     2761 ttctgcatat tgcatcagtt tatgtatatt cctgtcttta gatgtggcat atcttttcat
     2821 tgcttcacct acgatctgaa tatcgctgcc actaccacgc agaatgtcgc acaatgtccg
     2881 ttccaaatcg tatactcgaa tcggattacc tgacaaagac tcaatctcaa ttattcccga
     2941 ttgatagttt ttaggtataa cacgctttac tattatgttc tctttatgca aggacggagc
     3001 gttataaccc tttggaaaag tcatagtata ttttgcaggt gtacggtccg aatagccaag
     3061 cagatacaaa gctgtatcat gcgaataaat accgcgtccg tatttgcatt gcagaagata
     3121 taaatcatct tcccatgcat tgcttttcac ataaataccg cgtccgtatc ggtatatttc
     3181 tccgctctct acaagattct gcaatacact acgatgcagt cctgcttccg agacttggga
     3241 tgcggtaatt attccgtctt ccaatgtatc caacaattca tcaatcttct cctttttgtc
     3301 catactaatc acctcactat gacaacaaca cattaaatat tagcaatata atgtgcgatt
     3361 gtcaatataa tagatataac ttgagttcgc aaattgcggt cttaagaatc ttaagatgaa
     3421 aaatattatt acttgatatt ccaaaacgga ttctcaggct taatctcaat taccctatct
     3481 atagccgata tgttataatt cagaaaagcc tctttaaagg cggtaagagt atcgttatta
     3541 agtaaaacgg attggagatt acatatcatg cgctatacct tatgtttgtt cgatcttgac
     3601 ggcacattga cagatccgaa gattggaata actaaatcat ttcaatacgc actttcgtat
     3661 ttcggtattc atgaagagct tgataacctt acaaatttta tcggtcctcc gttgcgggaa
     3721 tccttcaaaa acagttacgg attttccgat accgatacag aaaaagccgt agcaaaatat
     3781 cgggaatatt ttacggcaac aggtctctat gaaaatgccg tctaccctaa aatccctgaa
     3841 acattggaaa agttaaagaa taacgggtgt atacttgccg ttgctacgag caaagttacc
     3901 gcttatgcaa agaagatatt aaagcacttc aatcttgacg gatattttac tttggtcagc
     3961 ggtgatgaaa tggacggcag tttaacaaaa aacggaaaac atgctcttat taatattgct
     4021 ttggacagac ttgaccctga acgcactttg tcggttgtta tgattggcga ccgtaaagac
     4081 gatataatcg gtgcaaacaa tgcgggaatc gacagtatcg gcgtattatg gggatatggc
     4141 acagttgagg aacttgaaac ggccaatgct acaaagattg tccatacctc ggacgaattg
     4201 tactctctga ttattgaaga atgccggtaa atgca
//
LOCUS       DAOPWC010000083         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21206_length_4216_cov_3.593127, whole genome shotgun sequence.
ACCESSION   DAOPWC010000083 DAOPWC010000000
VERSION     DAOPWC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21206_length_4216_cov_3.593127"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKJ65_02700"
     CDS             complement(<1..191)
                     /locus_tag="PKJ65_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40779.1"
                     /translation="MKKRTAVKIVTSLLLVAMIGAFMVGCGTDSKSNTATPATSPEAS
                     KSTKNTNPIVVTWYPNESS"
     gene            complement(466..1314)
                     /locus_tag="PKJ65_02705"
     CDS             complement(466..1314)
                     /locus_tag="PKJ65_02705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNZ40780.1"
                     /translation="MKFTRNELLAFLKEQRKVFDIVRLVDVSLTTQYEITDSGELIQG
                     PYQCYTVWNKEKRCENCVSAKAFAIKGKLTKFEFVNNDIYFVTSIYTEVEGTPYMLEM
                     VDKLNDDVLFGAYGKDKFVDTITEHNRKLYIDTLTGAYNRLYYSEQLQKLTNIKVIAM
                     FDVDNFKTVNDTYGHPVGDYVLQEIVRIIIKNIRKSDAVIRTGGDEFLLILQDIPYEK
                     LSERLENIRKKIECIRCENCPDLRVTVSIGAIYADNDVTSLMEMADKKLYESKKNKNT
                     VTIARH"
     gene            1643..>4216
                     /locus_tag="PKJ65_02710"
     CDS             1643..>4216
                     /locus_tag="PKJ65_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40781.1"
                     /translation="MKKLSFRRNKNLIKKSAIIMLIGVLLFAGMPNKVQSQGSYDTNF
                     SWTTDEEVLNEWTLDPAIKSTSYTGIGTAGFSYHHYYYDEQDRIVLEFELSMFPASAG
                     RELSASRLFRENWQYAHIFIDPKLINKVDVAASFFQMYYPKDPTTVSFNQGDNTGTGN
                     VYKFTIDQVYPQKPANSDYMKSKLYLILKDTVTREQLNDDYALELRYTNNSNQVYNQK
                     GANGRIVLGNYLNYFEELNNYDPSANNDDVTLKTIAPFHTASMSNLVANPYLPPDMMR
                     TVAQSVIYDSAQGKLHVYYKQAPNHYTYRNYANDGQFLSSWIGIRQVMDSRIYDALLP
                     DANGVVGQMRMTDLDGVPGTNWSVSTEIKVDEFSYIPTTPTVGTYSYMMVPAEFKTDV
                     VNKAVVPTNQKNNIKNVYLHGYRKQSDFVRFIYNVDKNKMDALFAQGNHSTLSISTAY
                     ITDRPTEKTQTEYRLTPAQNIVIPKGAKLVFELPETSRSVFKSGPNNNYERLIGNLKT
                     KVDGQMSTGVDPNDYGEGYTQTFYSGLIFGGHVLTPETGLTLRAGETASLTVFDTASP
                     NQVKMTVVTGMYNQNREEYILTKHAENENKIYNLAHSNVRTGIIINRSANTPHVDEFF
                     TDSASITGHSKYPLALVSVRKASDGVIFREANSSDTDQLFNAEGMPYNGYPFTLDIPS
                     DVSLKKDMALSFSNSAIGFMRSRASTYRAQAKVTFDKNDGTGNTVVRIVPINQKAYND
                     PGYSPNGFDGPNILKVSQGTIEYLSDYEGKALTKEADIVERTFYSGPTRAGYNFLGWS
                     TKQVDSMGATTFADLPTLTDVAQWESTDNYKFTATSPVDQGRTVYAVWEKNTIEQYKI
                     VL"
BASE COUNT         1298 a          803 c          818 g         1297 t
ORIGIN      
        1 tccgatgatt cgttgggata ccacgttaca actatgggat tagtgttttt ggtgcttttg
       61 gaggcctccg gcgaagtggc gggagtggcg gtgttgctct ttgagtcagt accgcaaccg
      121 accataaaag ctcctatcat tgctacgagt aaaagtgatg ttactatttt tactgctgtt
      181 ctttttttca tttttctttc tcctttaatt ttctaatcat tttcaaattt cgttttgatt
      241 gtacaattga catgtaaaat ctgacacctt attcacgtca aaagtaaata aaaagagtgg
      301 taggtatctt aaaattatgt atactccgag aagccggaat cacatacgga atgttttcag
      361 gttatcggag tgttggatta gatgagaaaa acattcgttt ttcgtatgta atacggaaaa
      421 ttgaaaattt tacagtgtag aatacactac atgaaaaaaa acaccttaat gtcttgctat
      481 tgtaacagtg ttcttatttt tcttagattc atacaatttt ttatcagcca tttccatcag
      541 tgaagtaaca tcattgtcgg catatatggc ccctatgctg acagtaactc ttaagtccgg
      601 acaattctca catcgaatac attcaatttt tttgcgtata ttttccagtc tttcagaaag
      661 tttttcataa ggtatatcct gtaaaataag cagaaattca tctccgcccg tgcggatgac
      721 agcatccgat ttgcgaatat tctttataat aatccttact atttcctgca acacataatc
      781 tcctaccgga tgaccgtaag tatcattaac tgttttgaaa ttatccacat caaacatggc
      841 aatgactttt atattggtta atttctgcaa ttgctccgaa tagtaaaggc gattatatgc
      901 accggttaaa gtatcaatgt acagttttcg attatgttcg gttattgtat ctacaaattt
      961 gtccttcccg tatgcaccga ataaaacatc atcgttaagt ttatcaacca tttccagcat
     1021 atagggagtg ccttcaacct ccgtatatat tgatgttacg aaataaatgt cattgttaac
     1081 aaactcaaac ttagtgagtt tccctttaat tgcaaatgct tttgcggata cacagttttc
     1141 acatcgtttt tctttattcc atacagtgta gcattggtat ggtccttgta ttagttcacc
     1201 ggaatcagtt atctcatatt gtgttgttaa tgatacatca acaagcctta caatatcaaa
     1261 tacttttctt tgttctttta aaaatgcaag taattcattt cgtgtaaatt tcataacttt
     1321 ctccaaatac ctttcatatg aactgtctct tcctattctt ttgttgactt catttttatt
     1381 atatgcaacc cgagttggtt aagcaaacaa cagtttattt gaaaatgttg tttattgttt
     1441 taatcggttt tttttgttat tttacattca acagcggata tcttgttgtg tttaaagcag
     1501 agatgtcgag tttttaagta aatttaacat aaatttacac cattattgat accaaatact
     1561 aaatagttca ttgcatatat tgaataaata aaatatttta tttataatat actgtgcata
     1621 agtagatgag aggtatggta gattgaaaaa attaagtttc agaaggaata aaaatttaat
     1681 aaaaaagtca gcaattatta tgctgattgg cgttttactc tttgccggca tgcccaataa
     1741 agtgcagtcc caaggttctt atgatacgaa tttttcctgg acgacagacg aagaggttct
     1801 aaacgaatgg actttagatc ctgcaattaa gtcaacgtca tataccggta tcggtacagc
     1861 aggtttttcc taccatcatt actattatga tgaacaagac aggattgtgc tggaatttga
     1921 gttaagcatg tttccggcat cagcgggtag agagctttca gcatcccggc ttttcagaga
     1981 aaactggcaa tatgcacata tttttattga tcccaagctt ataaacaagg tggatgtagc
     2041 tgccagtttc tttcaaatgt attatccgaa agatccgaca accgtttcct ttaaccaggg
     2101 agacaacacg gggacgggga atgtatataa atttactatt gatcaggtat atccgcagaa
     2161 gccggccaac agtgactaca tgaagtctaa actttattta atactgaaag atacggtcac
     2221 aagagagcag ttgaatgacg attacgcttt ggagttgcgt tataccaata acagtaatca
     2281 agtctataat caaaaaggtg caaacggaag aatcgtgctg ggaaactact taaactattt
     2341 tgaagaactt aacaattatg atcccagtgc caacaatgac gatgttactt tgaagacaat
     2401 agctcctttc cacactgctt ccatgagtaa tctggttgcc aatccttatc tgccgccgga
     2461 catgatgaga acggtggctc aatcggttat ttatgacagc gctcagggaa aacttcatgt
     2521 ttactataag caagcaccta atcactatac ctaccgcaat tatgcaaatg acggacagtt
     2581 tttatcctca tggataggaa tacgtcaggt tatggactcc agaatatatg atgctttact
     2641 gcccgatgcc aacggggtag tgggtcagat gagaatgacg gatttggacg gagttcccgg
     2701 aactaattgg agtgtatcta cggaaataaa agttgatgaa ttttcctata taccgacaac
     2761 cccgaccgta ggtacctatt cttacatgat ggtacctgcc gagtttaaaa ccgacgtagt
     2821 gaataaggct gttgtgccta ctaatcagaa aaataatata aagaatgtgt atttacacgg
     2881 atacagaaaa cagtcggact ttgtacgttt tatttacaat gtggataaga ataagatgga
     2941 tgccttgttt gcacagggca atcactcgac tttaagcatt tccacagctt atataaccga
     3001 caggcctacg gaaaagacgc agacggagta cagattgact cctgctcaga atattgtcat
     3061 tccgaaagga gcaaaacttg tatttgagtt gcccgaaact tcgcgttccg tttttaaatc
     3121 cggtccgaac aataactatg aacgtttaat aggtaattta aaaacaaaag tagacggtca
     3181 gatgagtacg ggtgtagacc ctaacgacta cggtgaaggt tatacacaaa cattctattc
     3241 cgggctaata tttggcggac atgttttgac acccgaaacg ggtctgacac tccgggcggg
     3301 agaaaccgca agtttaaccg tgtttgatac tgcatcgcca aatcaggtaa aaatgacggt
     3361 agttaccgga atgtacaatc agaacagaga ggaatatatc ttaacaaaac acgcggaaaa
     3421 tgagaataaa atatataacc tggctcattc caatgtgcgt acgggtatca tcataaaccg
     3481 cagtgcaaac actcctcatg ttgacgagtt tttcacggac agtgcatcta ttaccggaca
     3541 cagtaaatat cctttggctc ttgtgtcggt aagaaaagca tcggacggag tgattttcag
     3601 agaagccaat tcttccgata ctgaccaact gtttaatgct gaaggaatgc cgtataacgg
     3661 ttatcctttt actctggata taccttccga tgtgagctta aaaaaagata tggctctgtc
     3721 cttttccaat tcggcaatag gttttatgag atccagagca tccacctaca gagcccaggc
     3781 aaaagtgaca ttcgataaaa atgacggaac aggtaacact gtggtgcgta ttgtgcccat
     3841 aaatcaaaag gcttacaatg accccggtta cagccccaac ggttttgatg gacccaatat
     3901 tctgaaggtt tcccagggta cgattgaata tctttccgat tatgaaggca aggcccttac
     3961 taaagaggcg gatattgtag aaagaacttt ctattccggt ccgacacgtg ccggttacaa
     4021 tttcctgggt tggtccacca agcaggtgga ttccatggga gcaacgacct ttgccgattt
     4081 gcccacactt accgatgtgg cacaatggga aagtaccgat aactataagt tcactgcaac
     4141 ctctcccgta gaccagggca gaaccgtata cgcggtttgg gaaaaaaaca ctatagaaca
     4201 gtataaaata gtgctt
//
LOCUS       DAOPWC010000084         4184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21428_length_4184_cov_3.389683, whole genome shotgun sequence.
ACCESSION   DAOPWC010000084 DAOPWC010000000
VERSION     DAOPWC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21428_length_4184_cov_3.389683"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1209)
                     /locus_tag="PKJ65_02715"
     CDS             complement(103..1209)
                     /locus_tag="PKJ65_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ40782.1"
                     /translation="MMASKVYFTDLHTKHGHNLLTKFENLFKKAGIDELSFENKFTAI
                     KIHFGETGNLAYIRPNYTKVLSDYIRSKKGRPFLTDCNTLYPGSRKNALEHLDTAYQN
                     GFNPFVTGCNVIIADGLKGTDEALIKIDLKHVKEAKIGHAIADSDIIISLNHFKGHVS
                     TGMGGAVKNIGMGSGSRAGKMEMHSAGKPEVKKEKCRGCFTCIKSCAHDAISEVYGKA
                     YINHDYCTGCGRCIGSCLFDAIRPSHDETNEILSEKMAEYAYAVLKDKQHFHISFVTD
                     ISPYCDCHSFNDVPIVDDIGIFASFDPVALDKACADAVNEGIVIKGSLLDKNRKMKDN
                     LTTVNPSTDWIHALQYASDIGLGSMQYDLIKLDK"
     gene            1379..2677
                     /locus_tag="PKJ65_02720"
     CDS             1379..2677
                     /locus_tag="PKJ65_02720"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNZ40783.1"
                     /translation="MDFIEKRLLECAVHVPKILIPTNKSDHISWPGFPAVNDDLNASL
                     LAPLNAGNLMNSKDYSSDLENELNLPERKRYVRTYKNMKRAVRSGFLTEYKGFILTRR
                     INKSGVERYGLLILLDYEDYLSDNPIIAVSREYNRDIATAQADFMTNAIFDLQYAQAL
                     IEDQEATLIEPLVASTFHFKKIFSFNHPVFGIYEGFLIDTPQHFTLISNAFAKIKAKS
                     IKNNKSLFLVHEGVESFESGLIHWNRLKEQYGNRLYRYNPARFHLVELFNIYSPDINL
                     IPYNVLVKDISHDKLVTQFRAKEKIKVETLSSVRELHEAILKRSEKYGSVCYGLICDD
                     VSYLVTFRTSESDIGIINIQDTLAKLMQENEHSVSYHINISEMLIEYAEGKHTAIIMP
                     PIKKDTFFDNIAKHGRLNRHAYCHDIPANLRFLFEARFLQ"
     gene            complement(2678..4117)
                     /locus_tag="PKJ65_02725"
     CDS             complement(2678..4117)
                     /locus_tag="PKJ65_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ40784.1"
                     /translation="MKVVLFALNSSYVHTNLAVRCLKNYAEQQLPDKHEIIIVENNLK
                     DKFDEVLYSLYSLKADVYAFSCYIYNIREILFYAGGLKSLLPDSLIILGGPEVSYDAE
                     DLLKNHTFIDYVIINEGEEVFAEFLEKGSDEKIIDGKIFGKFTQTGIQYSVNETISGN
                     IAYYESSRGCPYKCSYCLSSRISGIRAKTVSHTLQDLLEFEKFSTVSTIKFVDRTFNY
                     DMKRAKDIFQGLLSEKYTKTYHMEMRPELFDEEMFCILEKFPKGKLQMEIGIQSTDNK
                     VLLAIDRPSDIEKAIRNMKKLRKLGNINVHADLIAGLPYDDISSVRESYNSLFYACDR
                     LQLGFLKLLKGTKIRKEKDKYAYRFNMNAPYDVYENRFISFDEISSLKRVAALTERYK
                     NSVHFERTIEYITGFSLSPFDFFVGLDRYINRHISSVSQENAFKALYEFSKKEIKSGM
                     TDKEWSDFIDYLKEDYSGCLNRQAPVSYI"
BASE COUNT         1268 a          847 c          719 g         1350 t
ORIGIN      
        1 ttcaatttgg gatttctctt ttattctatc aatccccaaa caagtttaca ctatcgctgc
       61 atgtatgtcg ttaaaatatt gtaaaaaaca tgcctaacaa gcctatttat ccagtttaat
      121 taaatcgtat tgcatactgc ccagtccgat atcgcttgca tattgcaaag catgaatcca
      181 atcggttgag ggattaaccg ttgtaaggtt atctttcatt tttctgtttt tatccaacag
      241 actgcctttt ataactattc cttcattaac ggcatcggca catgccttat ccaatgcaac
      301 cggatcaaaa gaagcgaaaa tacctatatc atccacaatg ggaacatcat tgaaactgtg
      361 acaatcgcag tacggagaaa tatccgtcac aaaggatatg tggaagtgtt gtttatcttt
      421 caatacggca tatgcgtatt ccgccatttt ttcactcaat atttcgtttg tttcgtcatg
      481 tgaaggtctg atggcatcaa acagacagga gccgatgcat ctgccgcaac cggtacaata
      541 atcatggttt atgtatgctt ttccgtaaac ttcggaaata gcatcatgag cacaactttt
      601 tatgcatgta aaacaacctc tgcacttttc tttttttact tcgggtttac ctgccgaatg
      661 catttccatc ttacccgctc tggaacccga ccccataccg atattcttga cagcacctcc
      721 cataccggtg gaaacatgtc ctttaaaatg gttaagggaa ataataatgt cggaatcggc
      781 aatggcatga cctatctttg cctcttttac atgctttaaa tctattttaa tcaatgcttc
      841 atcggtccct tttagtccgt ccgctataat aacattgcat cctgtgacaa agggattgaa
      901 tccgttttga taagcagtat ccagatgttc caatgcattt ttcctgcttc ccggatacag
      961 tgtgttgcaa tcggtcagaa aaggccttcc ttttttactt ctgatatagt cgctcagaac
     1021 ctttgtataa ttgggcctta tatacgccag attacccgtc tctccgaaat gtatttttat
     1081 agcggtaaac ttgttttcaa atgataattc atcaattccc gccttcttaa aaaggttttc
     1141 aaatttggtc aacagattgt gaccgtgttt ggtatgcaga tcggtaaagt atacttttga
     1201 tgccataatg gttgtcacct cttttatagg ataattataa cgggttactg cgtgcaattc
     1261 aaatcgtaca tattctcaaa agaagaatac aaaggttggt tttgctgttt ttattaatat
     1321 tatgctgtgg ataatccgct gaccgtatgg taatattatt catgaaaagg aactgaaaat
     1381 ggatttcata gaaaaacgac tgctggaatg tgcagtgcat gtaccgaaaa ttcttatacc
     1441 gaccaataaa agcgaccata tatcctggcc gggttttcct gcggtcaatg atgatttgaa
     1501 tgcatcttta ttggctcctc ttaatgcggg gaacttaatg aattccaaag actattcttc
     1561 ggatttggaa aatgaactca atcttcccga aagaaagcga tacgttcgga cttacaagaa
     1621 catgaaaaga gctgtacgtt cgggtttttt aaccgagtat aaaggattta ttctgacaag
     1681 acgaataaat aagtcgggtg tcgagagata cggattgctt attttgctgg attatgaaga
     1741 ttatctttcc gacaacccga taattgcggt ttcaagggag tataacaggg atatagctac
     1801 cgcacaggcc gattttatga caaacgcaat cttcgattta cagtatgcac aggcccttat
     1861 tgaagatcag gaagcaaccc ttatagaacc tttagtggca agcacttttc attttaagaa
     1921 aatattttct tttaatcatc ccgttttcgg aatatatgaa ggatttttaa tcgatacacc
     1981 gcagcatttc acacttataa gcaatgcttt tgcaaaaatc aaggctaaaa gtatcaaaaa
     2041 taacaaatca ctttttttgg ttcatgaagg agtggaatct tttgaaagcg gtcttattca
     2101 ttggaacaga ttgaaagaac aatacgggaa ccggctttac agatataacc ccgcacgttt
     2161 ccatcttgtg gaattgttca acatatacag tcccgatatc aaccttattc cgtacaatgt
     2221 actggtaaaa gacatttctc atgacaagct tgtaactcaa ttcagagcaa aggaaaagat
     2281 taaggtggaa acattatctt cggtaagaga actgcatgaa gccatattga agcgatcgga
     2341 aaaatacggc tcggtttgtt acggacttat ctgcgatgac gtgtcctatc tggttacttt
     2401 cagaacttcc gaatcggata taggtattat aaatattcag gatactttgg ccaagctcat
     2461 gcaggagaat gaacattcgg tctcctacca tatcaatatt tcggaaatgc tcattgaata
     2521 tgccgaagga aagcatacag ctatcataat gccgccgatt aaaaaagata catttttcga
     2581 caatattgcc aagcacggac ggttaaaccg acatgcatat tgtcatgaca tacccgctaa
     2641 cttgagattt ttatttgaag caagattctt acaatagtta aatataactc acaggtgcct
     2701 gtctgtttaa acagccactg taatcttctt tcagatagtc tatgaaatcc gaccactctt
     2761 tatcggtcat accgctttta atctcttttt ttgaaaactc atacagagct ttgaacgcat
     2821 tttcctgaga aacggaagat atatggcgat ttatatatct atccaatccg acaaagaaat
     2881 caaacggact taaagaaaat ccggttatat actcaattgt tctttcgaaa tgaacactgt
     2941 ttttgtatct ttcggtcaat gcggctactc ttttcagact acttatttca tcgaaagaaa
     3001 taaagcgatt ttcataaaca tcatatgggg cattcatatt aaacctatat gcatatttgt
     3061 ccttttcttt gcgtattttt gttcccttaa gtaacttcaa aaatcccagt tgcaaccggt
     3121 cacaggcata aaacaatgag ttatatgact ctcttaccga agatatatcg tcataaggta
     3181 atcccgcaat caggtccgca tgaacattga tattacctaa ttttctgagt tttttcatat
     3241 ttcttattgc cttttcaata tccgaaggtc tgtcaatggc caaaagaact ttattgtcgg
     3301 tactctgaat accaatctcc atctgcagct tacctttagg gaatttttcc agtatacaaa
     3361 acatttcttc atcaaataac tccggacgca tttccatatg ataggttttt gtatatttct
     3421 cacttaacag gccctgaaag atatcttttg ctcttttcat atcataatta aaggtcctat
     3481 ccacgaattt aatagtggag acagtactga acttctcaaa ttccagaaga tcctgcaatg
     3541 tatgagaaac cgttttagct cttatgcctg aaatgcggga agataagcaa tatgagcact
     3601 tgtaaggaca acctcttgaa gactcataat atgcaatatt acccgaaatt gtctcattta
     3661 cggagtattg tatacctgtt tgtgtaaatt taccgaatat cttaccgtct attatttttt
     3721 catcactgcc cttttccaga aactcggcaa acacctcttc cccttcattt attatgacat
     3781 aatcaataaa tgtatgattt ttcagtaaat cctccgcgtc ataagatact tccggtcccc
     3841 ccaatattat caacgaatcg ggaagaagcg attttaaacc gccggcatag aaaagaatct
     3901 cacggatatt gtatatgtag caggaaaagg catacacatc cgccttcagc gaatacagcg
     3961 aatatagtac ctcgtcaaac ttatccttaa gattattttc cacaattatt atttcatgct
     4021 tatccggaag ttgctgctcg gcataatttt ttaagcatct tactgctaaa ttggtatgaa
     4081 cataggagct gttgagtgca aacaatacaa ctttcataaa acatttcctc gttttataag
     4141 atccttcccg aatttatctt taagttcgtc gatagtttct tcca
//
LOCUS       DAOPWC010000085         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21480_length_4176_cov_2.960689, whole genome shotgun sequence.
ACCESSION   DAOPWC010000085 DAOPWC010000000
VERSION     DAOPWC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21480_length_4176_cov_2.960689"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKJ65_02730"
     CDS             <1..635
                     /locus_tag="PKJ65_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNZ40785.1"
                     /translation="EHGGGNNSTFTTHVVTSSGTDTYSCVSASLGSLKGPRHGGANLK
                     VVHMMDDILKHVKVLSDDNIEVYLEKIIAKEAFDKSGLIYGLGHAVYSVSDPRALILR
                     NQLEKYMSDKKRDRLFTLYDKLQYIAPKVIQKKRNVTGNIAINVDYYSGYLYRQLDIP
                     EELYTPIFAISRILGWSAHRIEELANNGKIIRPAYIAVAERSKYVDIDER"
     gene            684..1577
                     /locus_tag="PKJ65_02735"
     CDS             684..1577
                     /locus_tag="PKJ65_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNZ40786.1"
                     /translation="MVIVLFIIMLIGLLLGKMKVLNSESRNAISFLIINVSMPMLIVV
                     SSNLEFDRQTTNEAMWLTIMSVATFFFSILLAQLIFKKAVPRFAMVFSNAGYMGLPVL
                     NSILGAKGLFLGAIFQMIFHVFTWTYGISLFTKSGKFKDSLKKMLNPCLVAVVIAVIF
                     MLTGWEIPDTIRTVLKMMGELTTPLSMLLIGATLSQVNFKEIIKVKEIYLVTALRLLV
                     IPAFVLIFFLFPSIPAISVYVVFVINAMPSAAITGILAMKYNNDEKLASSIVAFSTLV
                     SIVTLYLITDVFDVAALLSLI"
     gene            1737..3080
                     /locus_tag="PKJ65_02740"
     CDS             1737..3080
                     /locus_tag="PKJ65_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ40787.1"
                     /translation="MRKIKAFRSLALLLALCMIAALFAGCAKKANPLTIWVGVESVDF
                     YTAKMAEYVENYKTKTGEDFPYQIAVQGVDTGSAASKVLDDIDAGADIFTIAHDNLPK
                     LTAGASAIAPVRSQALLNQIKSDNPQAFLDVIDMNVQGTTYTFGIPYIAQSLILMYNT
                     KYLTKDDVKTWEGIWAAAKKHNMQSVSLTGSDGFNNSFLVLAKNETTKKMPTVLYEDG
                     ITENCNFTSDEVVAIMQWGQRFFTGANGAKMPTDSGWEIELRDEISLGVITGAWKFNA
                     AKAALGANLGIATLPSFTLTAEDVKGTNVKAGTVYRSGTFADCKVFVMKKGSDKADYL
                     EDILLFLTSKEVQEQSFIECANLPAYKNAVKEFASMQTDNLETELAARQLDMFDYGIP
                     QPFGTPTSQFYYYSKGAPDLIREILENKDGLFGTTDAIRAQLQKVENIWKTGERE"
     gene            3106..>4176
                     /locus_tag="PKJ65_02745"
     CDS             3106..>4176
                     /locus_tag="PKJ65_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ40788.1"
                     /translation="MDRKKYGISGFFQRFAQPFVNFYKCFMDGSIGTKMSHFIMGAGN
                     IYHGQIIKGLIYFLIQVGYVVFMVTCPKVNNTPLGYKAFINFVTLGTQEGDIFTPADN
                     SMLMLLFGVVTFGITLMYLFAYNSSIKSSYKADKDLKNYGKVTTFRHDLKEMFGNRFY
                     IAMLTPATLGAVVFTLLPTLFMILIAFTNFDKERQAGLKLFDWVGLDNFTTFFTNPSG
                     EIAARFLPVLGWTLIWAFFATFLNYYGGILLALLINKKGLKGKVIFRAIFVLTIAIPQ
                     FISLLAMRNLIGEYGPLQSFLIKTGIMSEPMSFLANAPNVWIARITIILINLWVGIPY
                     SMLLASGAIMNIPESLYEAARID"
BASE COUNT         1244 a          844 c          880 g         1208 t
ORIGIN      
        1 cggagcacgg cggaggtaat aactccacat tcaccactca tgttgttaca tccagcggta
       61 ccgataccta ttcctgcgta tccgcttctc tcggttcatt gaaaggacct cgtcacggag
      121 gtgccaatct taaagttgtt catatgatgg atgacatatt aaaacatgtg aaagtacttt
      181 cggatgacaa tattgaagta tatctggaaa agattattgc aaaagaagca tttgacaaat
      241 ccggtctcat atacggactg gggcatgcgg tctactcggt atccgatccc agagcactta
      301 ttttacgcaa ccagttggaa aaatacatgt ccgataagaa aagggacaga ttattcactt
      361 tatacgataa acttcaatac atagctccta aagtaataca gaaaaagcgg aatgtcacag
      421 gtaacatcgc tataaatgtt gattattact ccggatatct ttacaggcag ctggatattc
      481 ccgaggaatt atatacacct atttttgcaa tctccagaat tttgggctgg tcggctcaca
      541 ggatagaaga attggccaac aacggtaaga taatacgtcc cgcatatatc gcggttgccg
      601 aacgcagtaa atatgtggat attgatgaga gataagctta ttgatgttgc atgtaaagtt
      661 ttttgatata taataccgat attatggtta tagttctttt tattatcatg ttaatagggc
      721 tgctcttagg aaagatgaaa gtattaaatt ccgaatccag gaatgcaatc tcttttctta
      781 tcataaatgt ttccatgccc atgctcatag tcgtatccag caatctggag tttgaccgac
      841 agactacaaa cgaagcaatg tggttgacta taatgtcggt tgcaactttc ttcttttcca
      901 ttctgctggc tcagcttata ttcaaaaaag cggttcccag atttgccatg gttttttcca
      961 atgccggtta tatgggattg cccgtactta actccatttt aggtgcaaaa ggcttattct
     1021 taggtgcaat atttcagatg attttccatg ttttcacatg gacatacggc atctcactgt
     1081 tcactaaatc gggtaagttt aaagactctc tgaaaaaaat gcttaatccc tgtcttgttg
     1141 cagtggtaat cgccgtcatt ttcatgctga caggttggga aatacccgat acaatacgaa
     1201 cggttttgaa aatgatgggt gaactgacta cccctctttc catgcttctc ataggagcca
     1261 cactttcaca ggtcaacttt aaagagataa taaaagtcaa agaaatatac cttgtaacag
     1321 ctttgcgcct gctggtgata cccgcattcg tgctgatatt ctttctgttt ccgtcaatac
     1381 ccgcaatatc cgtttatgtt gtattcgtta taaatgccat gccgtctgcg gccataacgg
     1441 gaatattggc aatgaaatac aataatgacg agaaacttgc ttcttcaatc gtggcttttt
     1501 ccacactggt atccatagtc actctgtacc tcattaccga tgtttttgat gtggcggccc
     1561 tgctttcttt aatttgaaaa acgattatct tactcttttt ttatataaat ttctcttaaa
     1621 gtaattgtaa attaaaactg taacaatatt tgacacctac tattcaatgg atgtataatt
     1681 tgtatgttaa tagaacataa aatcaattag atttatattt ttcaggagga gtgaatatga
     1741 gaaaaataaa agcgttcagg tcattggcat tgctattggc cttatgtatg attgcggcat
     1801 tatttgccgg atgcgcaaaa aaagccaacc ccctcaccat ctgggtgggt gtggaatcgg
     1861 tagattttta cactgccaaa atggcggagt atgtggaaaa ttacaaaacc aagaccggtg
     1921 aagatttccc ctaccagatt gcggtgcagg gagtggacac cggttcggca gcatcaaagg
     1981 tgctggatga tatagatgcc ggtgccgata tctttaccat tgcgcatgac aatctgccca
     2041 aacttacggc aggtgcatcg gcaatagctc cggtcagaag ccaggcatta ttgaatcaga
     2101 taaaatccga taatcctcag gcatttctgg atgttatcga tatgaatgta caaggcacaa
     2161 catacacctt cggcattccg tatatcgcac agtcgcttat cctgatgtat aacaccaaat
     2221 atctgacaaa agacgatgtt aaaacatggg aaggaatatg ggcggctgca aaaaaacata
     2281 atatgcagtc ggtctctctt acgggatcgg acggatttaa taattcgttt ttggttcttg
     2341 caaaaaatga aacgaccaaa aaaatgccta ccgtattgta tgaagacggc attaccgaaa
     2401 actgtaattt tacttccgat gaagtggtgg caataatgca gtggggccaa cggttcttta
     2461 ccggtgctaa cggcgccaag atgcctaccg actcgggatg ggaaatagag ttaagagacg
     2521 agatttcctt aggtgtcata accggtgcat ggaaattcaa tgcggcaaaa gctgccctgg
     2581 gagcaaatct cggaatagcc acacttcctt cctttacatt gacagcggag gatgtcaagg
     2641 gaacaaatgt caaggcgggt acggtataca gaagcggtac ctttgccgac tgtaaagtct
     2701 ttgtcatgaa aaaaggaagc gataaagccg attatctgga agacatattg cttttcttga
     2761 caagcaagga agtacaggaa cagtctttca tcgaatgcgc caatcttccc gcatacaaga
     2821 atgccgtcaa ggaatttgcc tccatgcaga ccgacaatct ggaaaccgaa ttggctgcaa
     2881 gacagctgga tatgttcgat tacggtattc cccagccctt cggtactcct acatctcaat
     2941 tctattacta ctcaaaagga gctcccgatc ttataaggga gatattggag aacaaagacg
     3001 gcttattcgg tacaacggat gccataagag ctcagttgca gaaagttgag aatatttgga
     3061 aaaccggaga aagagaataa aagtcggtcg aacggaggct aaaatatgga caggaaaaaa
     3121 tacgggatta gcggattttt tcaaagattt gcgcaaccct tcgtaaattt ctataaatgt
     3181 tttatggatg gcagtatcgg cactaaaatg tctcacttca taatgggtgc gggaaacata
     3241 taccacggtc agataataaa agggcttata tactttctta ttcaggtcgg ttatgtcgtc
     3301 ttcatggtaa cctgccccaa agtcaacaac acgcctttgg gctataaagc attcataaac
     3361 ttcgttacat taggcacaca ggagggagat attttcactc ccgcggacaa ttccatgctg
     3421 atgctgcttt tcggagttgt cacattcggt attacactta tgtacctgtt tgcatacaat
     3481 tcatcgataa aaagcagcta caaagcggat aaagacctta aaaactacgg aaaggtcaca
     3541 acattcagac atgacttaaa ggaaatgttc ggcaaccgtt tctacattgc aatgcttaca
     3601 ccggcaacgc tgggcgctgt tgtatttacg ttactgccga ccttattcat gattcttata
     3661 gcttttacca attttgataa ggaaaggcaa gccggcttaa aacttttcga ctgggtggga
     3721 ctggataatt ttactacttt ctttaccaat ccttcgggag aaattgctgc acgattcctg
     3781 ccggttttag gctggactct tatatgggcg ttctttgcga cattcctcaa ttactacgga
     3841 ggaatactgt tggcattact tatcaacaag aaagggctca aaggtaaagt aatattccgt
     3901 gcgattttcg ttcttactat tgcgataccg cagtttattt cactgcttgc aatgagaaac
     3961 cttataggtg aatacggtcc gcttcagagt ttcctgatta aaacaggaat tatgagcgaa
     4021 cctatgtctt ttcttgcaaa cgctcccaat gtgtggattg caaggataac cattatactt
     4081 ataaacctgt gggtgggtat tccctactcc atgctattgg catcgggtgc cattatgaat
     4141 atacccgaaa gcctttatga agcggcaaga atagac
//
LOCUS       DAOPWC010000086         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21539_length_4167_cov_3.134241, whole genome shotgun sequence.
ACCESSION   DAOPWC010000086 DAOPWC010000000
VERSION     DAOPWC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21539_length_4167_cov_3.134241"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKJ65_02750"
     CDS             <1..706
                     /locus_tag="PKJ65_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40789.1"
                     /translation="QEPMTSLNPVFRIGMQVDEVITLHNPEMTPEDVAKRTVELLDLV
                     GIANSEGVYKMYPHELSGGMRQRVMIAMALACSPDLIIADEPTTALDVTIQAQILDLL
                     RTLKDKINSSIMLITHDLGVIAEMADLVVVMYAGKVVEKGTANDIFKNAKHPYTIGLM
                     ESKPVINKDVDRLYSIPGKVPNPINMPDYCYFKDRCEKCVAACSGHYPKEIKLSDTHY
                     VSCYLYVDEEVKNNGK"
     gene            696..1715
                     /locus_tag="PKJ65_02755"
     CDS             696..1715
                     /locus_tag="PKJ65_02755"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNZ40790.1"
                     /translation="MANKRLKEKTFEPITPDKKYLLQVNALKTYFPIKAGVFSRVKGY
                     VKAVDGVSFNIKKGQTMGLVGESGCGKTTVGRTILKLTPHTDGEVYFDGQAIFDLTKK
                     EIIPMRPKMQIIFQDPYSSLSPRLPVGEIIGEAVREHKLVPEDKYDDYITSIMNSCGL
                     QAYHKDRYPHEFSGGQRQRICIARALALNPQFVVCDEPVSALDVSIQAQIINLLMDLQ
                     EKYGLTYLFISHDLSVVEHISDTIGVMYLGSLVETGSKKDIFKDPLHPYTQALFSAIP
                     VPDPDYKMKRIILEGSIPSPANPPSGCKFHTRCRHCFERCKKEDPKPKDVGNGHVVCC
                     HLYDK"
     gene            1718..1969
                     /locus_tag="PKJ65_02760"
     CDS             1718..1969
                     /locus_tag="PKJ65_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40791.1"
                     /translation="MFKKKEYDDDDGRVIAPMNVEGMPWFQKGVGLQSANNEKTTPLT
                     RKETLSLLFNATLAGLTVALVLGLAAFLYILFCIHVWLR"
     gene            1974..2450
                     /locus_tag="PKJ65_02765"
     CDS             1974..2450
                     /locus_tag="PKJ65_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40792.1"
                     /translation="MKYFMWIILILFVAVLIFLGFLIASRVDYFMYEKQVVSFVAKGI
                     QEGAIVRYDGKSVLVNKYNFEVMCGKLLTITEREKIHKVKEYAKDREIIIEVDERNYV
                     VIMPLERSKAVYMETVLDGKRRYFYVSDKYRIYERVITYSRPEGFYGPNTLLDDSK"
     gene            2530..3156
                     /gene="udk"
                     /locus_tag="PKJ65_02770"
     CDS             2530..3156
                     /gene="udk"
                     /locus_tag="PKJ65_02770"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ40793.1"
                     /translation="MMDILVIGIAGGTGSGKTTLTNRLKERFGKDISVISHDNYYRSQ
                     DDISFEERVKTNYDHPDSFETDLLIEHLKQLKNGHSIECPVYSFSEHTRTKDTRKIDP
                     TKVIIVEGILIFQNPELLNMLDIRIFVDTDPDVRILRRILRDVKERGRSLDSVVTQYL
                     ATVKPMHEQFVEPSKRNADIIVLEGGYNLVALDMIIQRIQGHVEATNE"
     gene            3149..>4167
                     /locus_tag="PKJ65_02775"
     CDS             3149..>4167
                     /locus_tag="PKJ65_02775"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNZ40794.1"
                     /translation="MNKTRYIYSKAGQLLYDEVARDLPIADYHCHLSPKEIYEDKPFD
                     NIGVMWLEKDHYKWRLMRAFGIQEQYITGKAGWKDKFFAYCKAIEHSPGNPLYHWSAM
                     ELKKYFDTDTVISEENAQTIWDKANAVIKQKKLSPRKLITSSNVIYIATTDDIVDDLQ
                     YHKLIKADKSFKTVVAPTFRTDNVLLVNKPDYAEYIKRLEKVSKTNITDLKSLCEAVE
                     KRLDYFAKQGCSLSDVGIQYEADADFDYATADRAFKHALKGKEVEMGDYKKFLGYMFV
                     YLSGQYKKRDITQQIHTGVYRNTNTRLFESIGPDCGCDISSNSLDAVSFVRLFVPIEQ
                     VKMVNS"
BASE COUNT         1370 a          756 c          953 g         1088 t
ORIGIN      
        1 ccaggaaccc atgacaagtt taaatcccgt tttcaggata ggcatgcagg tggatgaggt
       61 aataactctt cataatcccg aaatgacgcc tgaggatgtt gcaaaacgaa cggtggagtt
      121 gctggatctt gtgggtatag ccaattcgga gggtgtatac aaaatgtatc ctcacgaatt
      181 atcgggcggt atgagacaaa gagttatgat agcaatggct ctggcttgca gtcccgacct
      241 tataatagcc gatgagccca caaccgcact ggatgttacc atacaggccc agattctgga
      301 tctcttaaga acattaaagg ataagattaa cagcagcata atgctcataa cacatgatct
      361 gggcgttatt gccgagatgg cggatctggt agtggtaatg tatgcaggaa aagtggtgga
      421 aaaaggaacg gcgaatgata tattcaaaaa cgccaagcac ccctatacca taggacttat
      481 ggaaagcaaa cccgttatta ataaggatgt ggacagactg tacagcatac ccggtaaagt
      541 tcccaatccc ataaacatgc ccgactactg ttatttcaaa gacagatgcg aaaaatgcgt
      601 tgcggcatgt tcaggtcatt atcctaagga gattaaactt tccgatactc attatgtaag
      661 ttgctatctg tatgttgacg aggaggtaaa gaataatggc aaataaaaga ttgaaggaaa
      721 agacattcga gccaataact cctgataaaa aatatttact ccaggtcaat gccttaaaaa
      781 catatttccc gattaaagca ggtgtatttt cccgcgttaa agggtatgtt aaagctgttg
      841 acggagtttc attcaatatt aaaaaaggtc agactatggg gctggtagga gaatcgggat
      901 gcggtaaaac aacagtcgga cgtaccattc tgaaactgac gcctcatacc gacggtgaag
      961 tatattttga cggtcaggca atatttgact taaccaaaaa agaaataatt cccatgcgtc
     1021 ccaagatgca gataatattc caggatcctt attccagtct ttcgccgcgt cttcccgtag
     1081 gtgagataat aggggaggcc gtaagggagc ataaactggt accggaagat aaatatgatg
     1141 actatatcac atcgataatg aactcctgcg gactgcaggc atatcataaa gaccgatatc
     1201 ctcatgagtt ttccggcggt cagagacaaa gaatatgtat tgccagagcg ttagcactca
     1261 atccgcagtt tgtggtgtgt gacgagccgg tttccgctct ggacgtttcc attcaggcac
     1321 agattataaa tcttctgatg gatcttcagg aaaaatacgg tctgacatat ctgtttattt
     1381 ctcacgacct gtcggttgtt gagcatatat ccgatacgat aggggtaatg tacttaggat
     1441 ctttggtaga gacaggatct aagaaagata tattcaagga tccgcttcat ccctatacac
     1501 aggcattatt cagtgccata ccggtacccg atcccgatta caagatgaag aggataatac
     1561 tggaaggaag tattccttcg cctgcaaatc ctccttcggg atgcaagttc cataccagat
     1621 gcagacactg ctttgaaaga tgtaaaaaag aagatcccaa acctaaggat gtaggtaacg
     1681 gacatgtggt gtgctgccat ctgtacgata aatagagatg tttaagaaga aagaatacga
     1741 cgatgatgac ggcagggtaa tcgctcccat gaatgtggaa gggatgccct ggtttcaaaa
     1801 aggcgtcgga ttgcagagtg cgaataacga aaaaactact cctttgacaa gaaaggaaac
     1861 tttatcgttg ctttttaatg ccactttggc aggtcttacg gtagcgcttg tattgggact
     1921 tgccgcattt ttgtatattc tcttctgcat acatgtatgg ttaaggtgat atcatgaagt
     1981 actttatgtg gataatactg atactttttg tagcggtcct tatattctta gggtttttaa
     2041 tagcctccag agtggactat tttatgtatg aaaagcaggt tgtgtctttt gtagccaaag
     2101 gcattcagga aggtgctatc gtgcgttatg acggcaaatc ggtactggtt aacaaatata
     2161 attttgaagt catgtgcggt aaactcttga caataaccga aagagagaag attcataagg
     2221 ttaaagagta tgctaaagac agagaaatca taattgaagt ggatgaaaga aattatgtgg
     2281 tcataatgcc tctcgaacgc agcaaggccg tctatatgga aaccgtactg gacgggaaaa
     2341 ggcgatattt ctatgtatcg gacaaataca gaatatatga aagggtaata acttacagcc
     2401 gaccggaagg tttttacggc cccaatacat tgcttgacga ctcaaaatga gttgtttaac
     2461 aacaaagccg tgttattcat cggttaaagg taatatataa tagatataat aaaactaaaa
     2521 gaggggttta tcatggacat actggtaatc ggaatagcgg gaggaacggg aagcggaaag
     2581 actacactta ccaacaggct aaaagaacga ttcggtaagg atataagtgt gataagccat
     2641 gacaattact accgctctca ggacgatata tcttttgagg aaagagttaa gaccaattac
     2701 gatcaccccg attcttttga aaccgaccta ctcattgaac acttaaaaca gttaaaaaac
     2761 ggtcacagta tcgaatgtcc cgtttattcc ttttccgagc atacaagaac aaaggatacg
     2821 cgtaagatag atcccaccaa agttataata gtggaaggta tactgatatt ccagaacccc
     2881 gaactgctca atatgctgga tataaggata tttgtggata ccgatcccga tgtcagaata
     2941 ttaagaagga ttttacgcga tgtaaaagag agaggcagaa gtctggattc ggtggttacc
     3001 caatatttag ccacggttaa acccatgcac gagcagtttg tggaaccgtc aaagagaaat
     3061 gctgatataa ttgttttaga aggcggatat aatttggtgg ctctggatat gattatacag
     3121 aggattcagg ggcatgtgga ggcaacgaat gaataaaaca cggtatatat actcaaaagc
     3181 gggacaactt ttatacgatg aggtggcaag agaccttccc atagcggatt atcactgcca
     3241 cttaagtccc aaggaaatat atgaggataa gccttttgac aatatcggtg ttatgtggct
     3301 ggaaaaagat cattacaagt ggagactgat gagagctttc ggcatccaag agcaatatat
     3361 aacgggaaaa gccggttgga aagataagtt tttcgcatat tgtaaagcta tagagcactc
     3421 acccggcaac cctttatatc attggtcagc aatggaatta aagaaatatt ttgataccga
     3481 tacggttatt tcggaagaga atgcacaaac tatctgggac aaagccaatg ccgttataaa
     3541 acaaaagaag ttaagtccga gaaaactcat aacttcctca aatgtcatat acatagcgac
     3601 cacagatgat attgtagatg atttgcaata tcataaactt ataaaagcag ataagtcttt
     3661 caaaacagta gttgcgccga ctttcagaac cgataatgtg cttcttgtaa acaagccgga
     3721 ttatgcggag tatataaaga gactggaaaa agtatcaaag acaaatataa ccgacctcaa
     3781 atcgctgtgt gaagccgtag aaaagcgact ggactatttt gcaaaacagg gatgctctct
     3841 ttccgatgtg ggaatacagt atgaagcgga tgcggatttt gactatgcaa cagccgacag
     3901 ggcatttaag catgccctga aaggcaaaga agttgaaatg ggtgattata aaaaattctt
     3961 aggctatatg ttcgtatacc tttcgggcca atacaaaaaa agagatataa ctcagcagat
     4021 acataccgga gtatacagaa ataccaatac caggcttttt gaatcaatcg gaccggattg
     4081 cgggtgtgat atttcctcca actcattgga tgcggtgtct tttgtgcggt tatttgtgcc
     4141 aattgaacaa gtaaaaatgg taaattc
//
LOCUS       DAOPWC010000087         4165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21553_length_4165_cov_3.016302, whole genome shotgun sequence.
ACCESSION   DAOPWC010000087 DAOPWC010000000
VERSION     DAOPWC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21553_length_4165_cov_3.016302"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKJ65_02780"
     CDS             complement(<1..1071)
                     /locus_tag="PKJ65_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ40795.1"
                     /translation="MKKYHKDISILGKNVLAPRATSIPYSDVADAKKDIRGLSPYFKL
                     LNGKWNFKYCENEYDVDESFYLPEYDDEDWDIIPVPSSWQVLGYDKPQYINVRYPYPV
                     DPPHIPDINPVGLYRTIFVLPDSFTARNVIIHFGGVNSSFVLYINGQEVGYSQGSHMP
                     SEFDITKYVRQGANLIAVQVYKMSATSYLEDQDFYRLSGIFREVYLYSTHNNSIMDFT
                     VKATLNDIFVSILSSETKGITYVYELYDKEDVKIFGKSVKKPFIDMHVEGVLPWTSET
                     PNLYTLILQLKDAKGTVTDIRSCKVGFRTVSINDGIFMINNTPVKLKGTNRHDTYALT
                     GHAISREVMESDIVMMKRHNINT"
     gene            1266..2354
                     /locus_tag="PKJ65_02785"
     CDS             1266..2354
                     /locus_tag="PKJ65_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNZ40796.1"
                     /translation="MSNSKKKKTDKEKKALTAITVITVVVGIPLFVLFLSRYFKNNTG
                     ISTDTPVIQSPIATYQQTEIPAETVAPTQTVMPTETAAVQTPEPVPSSSINLNEIEIR
                     NMTSLKIYLESILKSDEGAHGLSFYNTVTEEYIGINDTELFVAASSTKVPMVMYVYNA
                     VEKEQLSFDDIVVYTENDYEEGTGIIIKGEFGDVYTIGELAEYAIIHSDNCAINMLIR
                     TCNEWYMVNYMNKLGAKVDYIKKRWKTCPYDLTLYYKELLRLVEENPEHYQKLMDDLC
                     SNKVGYWMSSKSVLPKDLQIAMKTGINTTLPTYNEATIVFAKHPYILSYCSTDIVIEE
                     STELHKKISKVIYDFMENGYAVGELKWK"
     gene            2366..3490
                     /locus_tag="PKJ65_02790"
     CDS             2366..3490
                     /locus_tag="PKJ65_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40797.1"
                     /translation="MTKKVSALIILILVMACLVSCNTGTVREPVQSSVLKPDTYYTSF
                     KISACFGRFIETPYGSYFASGNRYIYYSHKGNTKYVKLCNKPDCSHNSEDCNAFLKGS
                     VIGYYDNKIYYRRFKHIYCMDMDGCNHKIVKALYEEHCSNHGYFHNGYYYYIKTNFAE
                     YNGVKGKDDPTLYRTKVDDKSESEVLLTNDLILQTDMFTMIGDNIYISVRNSDPDKIS
                     SLYSYSTVTESLSKLTDDWSGLGSYLRDENKGYCYVKNKGFYEYDFGTNEMSLVKPIE
                     LENGGISGAFYLDDYILLVHNNSVPFDYENQVMCIYDRDYNLLNTIVFDKANGKIPGG
                     FLSDVDGYIIFSSNFRDKPDYYIDKSEIGTDNLQFHKIED"
     gene            3655..3963
                     /gene="rplU"
                     /locus_tag="PKJ65_02795"
     CDS             3655..3963
                     /gene="rplU"
                     /locus_tag="PKJ65_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ40798.1"
                     /translation="MYAIIETGGKQYKVSEGDTLYIEKIEAEEGKVKFDKVLAVATDE
                     GLKVGKPYVEKATVTAEVEKTGKAKKILVFKYKAKKGYKKMQGHRQPYTKVKITKISL
                     "
     gene            3982..>4165
                     /gene="rpmA"
                     /locus_tag="PKJ65_02800"
     CDS             3982..>4165
                     /gene="rpmA"
                     /locus_tag="PKJ65_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ40799.1"
                     /translation="MAHKKGVGSTKNGRDSQSKRLGVKRGDGQAVLAGTILVRQRGTK
                     IHPGENVGIGKDDTLYA"
BASE COUNT         1373 a          723 c          874 g         1195 t
ORIGIN      
        1 cgtatttata ttatgcctct tcatcatcac tatgtcgctt tccattactt cacgtgatat
       61 ggcatgtccg gtcaatgcat aagtgtcatg tctgttggtg cctttcagtt taaccggtgt
      121 gttgttaatc atgaatatac cgtcatttat cgaaactgtt ctgaatccca ctttacacga
      181 ccgtatatcg gtaaccgttc cttttgcatc ctttagttgc aggataaggg tatataagtt
      241 gggtgtctcg cttgtccagg gcagaactcc ctcaacatgc atatctataa agggtttctt
      301 tacggatttg ccgaatatct tgacatcctc tttatcatac agttcataaa catatgtaat
      361 gccttttgtt tcggatgaaa gaatactgac aaaaatgtcg ttcaatgttg ctttgaccgt
      421 aaaatccata atggaattgt tgtgtgtgga atagagatat acttcacgga aaataccgct
      481 cagtctgtaa aagtcctgat cttccagata actggtggca ctcatcttat acacctgtac
      541 ggctataagg ttggcaccct gacgaacata tttagtgata tcgaattccg aaggcatatg
      601 cgagccttgg gaataaccta cttcctgacc gttgatgtaa agaacaaaag aggagttaac
      661 accgccgaaa tgaattatga catttcttgc ggtaaaagaa tcgggcagta caaatattgt
      721 tctgtaaagt cccacagggt tgatatcggg aatatgcggg ggatcgacgg ggtacgggta
      781 tcttacatta atgtactgcg gtttatcgta tcccagtacc tgccaggatg aagggacggg
      841 tataatgtcc cagtcttcat catcgtattc gggaagatag aaagattcat ccacatcata
      901 ttcattctcg cagtatttga agttccattt tccgttcaga agtttaaagt aaggtgataa
      961 ccccctgata tctttcttgg catcggcgac atcgctgtaa ggtatcgaag ttgctcttgg
     1021 agcaagtaca tttttcccta aaattgatat gtctttgtgg tattttttca tggagacctc
     1081 ctcatatttt actgacagac ggttattgaa tcaatacatt caacaacaac attatatatc
     1141 ttacatcatg tatgtgaaag ttatgtgaaa aggaaacagg tttttaagca aacaagttta
     1201 aagtaaataa cttactgtta atatcagata attaatatgt ggtataatgc ttgaaggata
     1261 acactatgag caattcgaag aaaaaaaaga ctgataagga aaagaaagca ttaactgcaa
     1321 tcaccgttat aaccgtagtt gtgggtatac cgctttttgt acttttcctt tcccgatatt
     1381 ttaaaaacaa tacgggtatc agtaccgata ctcccgtaat tcagtcaccg attgctacat
     1441 atcagcaaac ggaaatacct gctgaaacag ttgctcccac acagaccgtt atgcctacgg
     1501 aaaccgcggc tgttcagaca cctgaacctg taccgtcatc aagtataaac ttaaacgaaa
     1561 ttgaaatacg aaacatgact tcactcaaga tttatcttga gtcgattctt aaatccgatg
     1621 aaggtgcaca cggattgagt ttttacaata cggttacgga ggaatacata ggcattaacg
     1681 ataccgaact gtttgtggcg gcatcatcca caaaagtgcc tatggtaatg tatgtatata
     1741 acgcagtgga aaaggagcaa ctttcctttg atgatatcgt ggtttatacc gaaaatgatt
     1801 atgaagaggg aacggggata ataatcaaag gggagttcgg agatgtatat acgatagggg
     1861 aattggcgga atatgccatt atacacagtg acaactgtgc gataaatatg cttattcgga
     1921 cctgtaatga atggtatatg gtcaattaca tgaacaaact aggtgccaag gtggattaca
     1981 taaaaaagcg ctggaaaacc tgtccttatg atcttacgct ttactacaaa gaattattaa
     2041 ggcttgtgga agaaaacccc gagcattatc aaaagcttat ggacgatctt tgcagtaaca
     2101 aggtgggtta ttggatgtct tccaagtcgg tactgcctaa agacttgcag attgccatga
     2161 aaaccggaat aaataccaca ttgccgacat ataacgaagc tacaatcgtt tttgcaaaac
     2221 acccttatat cttatcttac tgttcgacag atatagtaat tgaagaatct acagagcttc
     2281 ataaaaagat aagcaaggtt atatacgatt tcatggaaaa cggatatgct gtaggtgaat
     2341 taaagtggaa atagcaggta atcgtatgac aaaaaaggtt tctgccctca taattctcat
     2401 acttgttatg gcttgtctcg tatcgtgtaa taccggaacc gtccgggaac ctgtccaatc
     2461 ttctgtttta aagcccgata catattatac ttcgtttaaa ataagcgcct gtttcggtag
     2521 atttattgaa actccctacg gttcgtattt cgcttccgga aacagatata tatattactc
     2581 ccataaggga aacacgaaat atgttaaact gtgcaacaag cctgactgca gccataattc
     2641 ggaagattgt aatgcgtttt taaaagggag tgtgataggt tattatgata ataaaattta
     2701 ctacaggcgt ttcaaacata tatattgcat ggacatggac ggatgcaatc ataaaatagt
     2761 taaggcattg tatgaagagc attgtagtaa tcacggatat tttcataacg gatattatta
     2821 ctacattaaa actaatttcg cagaatataa cggagtaaaa ggaaaagatg accctactct
     2881 ttacagaaca aaagtagatg acaaatcgga gtccgaagta ttattgacaa atgatttgat
     2941 actgcaaacg gatatgttta cgatgatcgg agataatata tacataagcg tgcggaattc
     3001 cgatcctgat aaaatatctt cgttatattc ttattcgaca gttaccgaaa gtctctcaaa
     3061 actgaccgat gattggtccg gcttaggttc atatttaagg gatgagaata aaggttattg
     3121 ttatgtcaaa aataagggat tttatgaata tgatttcggc acaaacgaaa tgagccttgt
     3181 taagcccata gagttggaaa acggaggcat aagcggtgct ttttatttag atgattacat
     3241 acttcttgtt cacaataaca gtgtaccgtt tgattatgag aatcaggtta tgtgcattta
     3301 tgaccgggat tacaatctct taaataccat tgtatttgat aaagcaaacg gcaaaatacc
     3361 gggcggattc ttaagtgatg tggacgggta tataatattt tcttccaact tccgtgataa
     3421 acccgactat tacatagaca aatccgagat aggaacggat aatttgcagt ttcataagat
     3481 tgaagactga tttaggtgtt aaagttgttt aataacgaaa acacgggaaa aactcgttat
     3541 tgacacccat acctccttat gatagaatct gtcggtagcc gcacttgagg ggttttaaaa
     3601 gtatgcaatt acttaccttg gcaaggcgag attttgctta ggaggcaaca aaggatgtac
     3661 gcaattattg aaaccggagg aaaacaatac aaggtatccg aaggcgacac actctatatc
     3721 gagaagatag aggcggaaga aggaaaagtt aagtttgaca aggttcttgc agtggccaca
     3781 gatgaaggac ttaaggtggg aaaaccttat gttgaaaaag caacagtaac cgctgaagtg
     3841 gaaaagaccg gtaaggcaaa gaaaatactt gtttttaagt ataaggccaa aaagggatac
     3901 aaaaagatgc agggacacag acaaccttac acgaaagtaa aaattactaa aatttcttta
     3961 taggatcgga ggtgaattaa attggcacat aagaaaggtg ttggaagtac aaagaacgga
     4021 agagacagtc agagtaaaag attaggcgtt aaaagaggtg acggtcaggc ggttcttgca
     4081 ggaacaattc ttgtaagaca aagaggaacc aagattcacc ccggtgaaaa tgtcggaatc
     4141 ggcaaagatg atacgttata tgcaa
//
LOCUS       DAOPWC010000088         4150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21648_length_4150_cov_4.476190, whole genome shotgun sequence.
ACCESSION   DAOPWC010000088 DAOPWC010000000
VERSION     DAOPWC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21648_length_4150_cov_4.476190"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKJ65_02805"
     CDS             <1..409
                     /locus_tag="PKJ65_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNZ40800.1"
                     /translation="ICIKTLEHNMKTTKVRICNTEYVIKGDESEEYIEKIALFVDKKM
                     NSIIRENPKLSLSTAAVLTAVNVSYDYFKERDQANKQKDLMDEFEKKSKDQVSRVRLL
                     EAKIEKLTQANSELKETLTKTEEQLKKAQNEFR"
     gene            406..888
                     /gene="dut"
                     /locus_tag="PKJ65_02810"
     CDS             406..888
                     /gene="dut"
                     /locus_tag="PKJ65_02810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNZ40801.1"
                     /translation="MIKVYIEKTRKDAKIPVYANSFDAGADVCACEDAVLDINSTRVI
                     PTGLKVAIPEGYEIQIRPRSGLSLNTRLRVANSPGTIDAGYRDEIGIIMHNTSVVNEG
                     NFDVTEKHNRQGVYHIKKGDRIAQIVLQQIPKIEFVETDCVADIGKDRGGGFGSTGVR
                     "
     gene            866..1504
                     /locus_tag="PKJ65_02815"
     CDS             866..1504
                     /locus_tag="PKJ65_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNZ40802.1"
                     /translation="MARQGSDKAVYTIKQDAVSEITEKKSRFISYAIKTETEKDALAE
                     LEKIKSKYPDATHHVYAYVVNDQGVVYHRYSDDREPSGTAGLPTLDVLKKGNIENVLL
                     VTVRYFGGTLLGTGGLTRTYGLSAKKAVKQAGLVKRVDCAVFTLTADYSSYEKIKRLL
                     DECVVMDTEFGELVTVRFAAEDLKAEERIGRMNELLNSRCVIERKNNIVLEI"
     gene            1506..2237
                     /locus_tag="PKJ65_02820"
     CDS             1506..2237
                     /locus_tag="PKJ65_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ40803.1"
                     /translation="MAGHSKWANIKHKKEKSDAARGKIFTKIGREIMVAVKAGGPDPA
                     SNSRLRDVIAKAKANNVPNDTINKSIKKAAGEGDSENYEQITYEGYGPNGVAVIVEAM
                     TDNRNRTASDVRHYFDKFGGNLGTTGCVSYLFDRKGVLIIEEKESIDAEQLMTDALEG
                     GAEDFEHEDEMYEIICDPAEFSSLREALEQKGYEFLEAEVQMLPQTYVTVEEDKVESM
                     QKLIDNLEDLDDVQNIYHNWEQPEE"
     gene            2299..>4150
                     /locus_tag="PKJ65_02825"
     CDS             2299..>4150
                     /locus_tag="PKJ65_02825"
                     /inference="COORDINATES: protein motif:HMM:NF018444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40804.1"
                     /translation="MQKRINVSVRDIADFSLASGDIETYAAFGYREAALDGTQNHIRF
                     QKDMIKQYGEDCFAKEVYVEDVLENDVVYMVITGRIDGVLKTDDSNIFVYEIKTTCKD
                     INEIKPADRPEHLGQAHCYAYMYCKKNNIDATGIRLVYINRENQDSITFEYRLTFKQL
                     ESIYSELSNRYLDWVTGITAWKTIRNQSIETLEFPFASYRAGQKTLMSEVYNCIDDAV
                     KLFATAPTGIGKTMGVLFPAIKALGAGLVDKLMYLTAKTVTAKVAVDAYKKLEKNGLN
                     LRTVCITAKDKVCPMDKKDCDPEKCPRANHYYIRARACLKEILHEQFFDRDIIARYAD
                     EFNICPFELSLDVSGYCDLIICDYNYLFDPRVRLQRYFDYENEQSFCFMVDEAHNLVE
                     RGREMFSCSIGIKQLEDFKDSIKKEWKALRKSVNALINEMKELQAEHFSTMNQGFISQ
                     AELPLVLTDRANRCVNDIMMYDLSKIPDDKRESFLEGMFNILFFSRVAQMYDRRFTTL
                     FTSDGLGLDVKLMCLDPSYLLKQSMDTGRSAILFSATLEPKRYYMDLLGGDGNMDSFL
                     TLDSPFPRENLKVKLVTDISTKYKDRDLSYEKIAQLLKKQFSQKTGNYMVF"
BASE COUNT         1391 a          738 c         1031 g          990 t
ORIGIN      
        1 gatatgcata aaaacattgg agcataacat gaaaacgact aaagtgagaa tttgcaatac
       61 ggagtatgtg ataaaaggcg atgaatcgga agaatacatc gaaaagatag cattgtttgt
      121 tgataaaaaa atgaactcca taataaggga gaatcccaag ttaagccttt ctacggctgc
      181 cgtcttaacc gcggttaatg tctcttacga ttacttcaaa gaaagagatc aggctaataa
      241 gcagaaagac cttatggacg agtttgagaa aaagtccaaa gaccaggtgt cgcgtgtacg
      301 attactggaa gctaaaatag aaaagcttac acaagccaat agcgaactta aagaaacgct
      361 taccaagacc gaggagcagc ttaaaaaagc tcaaaacgaa ttcagatgat aaaagtttac
      421 atagaaaaaa ctaggaaaga tgcaaagatt ccggtttatg ccaacagctt tgatgccggg
      481 gcggatgttt gtgcatgtga agatgcggtt ctggatataa acagcacaag ggtgataccg
      541 accggtttga aagttgcaat acccgaaggg tatgagatac aaatccgtcc caggagcgga
      601 ctttctctga atacccgatt gagagtggcc aacagccccg gaactataga cgccggatac
      661 agagatgaaa tagggataat aatgcataat acctccgtag ttaatgaagg taattttgat
      721 gtaacggaaa agcacaaccg tcagggtgtt tatcatatta aaaaaggcga cagaatagca
      781 cagatagtgc tgcaacagat accgaaaata gagtttgttg aaaccgattg tgtcgccgat
      841 atcgggaaag acagaggtgg aggttttggc tcgacagggg tcagataaag cggtctatac
      901 aataaagcag gatgccgttt cggagataac ggaaaagaaa tcacgcttca tttcctatgc
      961 gataaaaacg gagacggaaa aagacgcctt agcagagctg gaaaagataa agtcaaaata
     1021 tcccgatgcc acacatcatg tgtatgccta tgttgtaaac gaccaagggg tggtttatca
     1081 cagatattcc gacgacagag aaccttccgg tactgcggga ctgcctacat tggatgtcct
     1141 gaaaaaaggc aacattgaaa atgtgctcct tgtaaccgta agatacttcg gaggaacact
     1201 tctcggaacg ggaggactga caagaactta cggtttaagt gccaaaaagg ccgtaaaaca
     1261 ggcggggttg gtaaagagag tagactgtgc cgtttttaca ttgacggcag actattcatc
     1321 gtacgaaaag attaagcgat tgttggatga gtgtgttgta atggatacgg aattcggaga
     1381 gcttgttacg gtgcgttttg ctgcggagga cttgaaagcg gaagaacgga ttggtagaat
     1441 gaacgagctg cttaatagca ggtgtgtaat tgaacgaaag aataatatag ttttggagat
     1501 atgatatggc aggacattca aagtgggcaa acataaagca caaaaaagag aaaagcgatg
     1561 ctgcaagagg taaaatattt accaaaatag gccgtgagat aatggttgcg gtaaaagccg
     1621 gaggaccgga ccccgccagt aattcgcgtt tgcgcgatgt tatcgctaaa gcgaaggcca
     1681 ataatgtgcc taacgatact ataaacaaaa gcataaagaa agcggcggga gagggagatt
     1741 cggaaaacta cgagcagata acatacgaag gatacggacc caacggtgtt gccgtcatag
     1801 tagaggcaat gaccgataac cgcaacagga ccgcttccga tgtgcgccac tatttcgaca
     1861 aattcggagg caacctcggg acgaccggct gcgtatccta cctgtttgac agaaaaggcg
     1921 tgttgattat agaggaaaag gaaagcattg atgcagaaca gcttatgacc gatgccctgg
     1981 agggcggagc ggaggatttc gagcatgaag atgaaatgta tgagataata tgcgatcctg
     2041 cggagttttc ttcattgaga gaagcattgg aacaaaaagg atatgagttt ctggaagcgg
     2101 aagttcagat gcttccgcag acatatgtca cggtagagga agacaaggtt gaaagtatgc
     2161 aaaagcttat tgacaatctg gaagaccttg acgatgtgca gaacatatat cataactggg
     2221 aacaaccgga agaataaaaa catagttgta caacacctgt cggtaagtgt ataatagaga
     2281 acaggtgttt gtttttttat gcagaaaaga attaatgttt cagtcaggga tattgcggac
     2341 ttttctctcg catcgggaga tattgaaacc tatgccgcat tcggttacag ggaagcggcg
     2401 ctggacggaa cgcaaaatca tatacgattt cagaaggata tgataaaaca gtacggtgaa
     2461 gattgctttg caaaggaagt atatgtagaa gacgtattgg aaaatgatgt agtgtatatg
     2521 gttattaccg gtcgaataga cggtgttctt aaaaccgatg attcaaatat ttttgtatat
     2581 gaaataaaga caacttgcaa agatataaac gagataaagc cagccgacag accggagcat
     2641 ctggggcagg ctcactgtta tgcatatatg tactgcaaaa agaacaatat tgatgcaaca
     2701 ggtataaggc tggtatatat aaacagagaa aatcaggaca gcattacttt cgagtaccgg
     2761 ctgactttta agcaattgga aagtatatat tccgagcttt ccaacagata tcttgactgg
     2821 gtaaccggaa taaccgcatg gaagacaata agaaatcaga gtattgaaac actggaattt
     2881 ccttttgctt catatagagc gggtcagaaa acacttatga gcgaggtata caactgcata
     2941 gacgatgccg taaaactttt tgccaccgct ccgaccggta tcggaaagac aatgggagta
     3001 cttttccccg caataaaagc attaggagcg ggccttgtgg acaagcttat gtatctgacg
     3061 gcaaagaccg taacggcaaa ggtggcggtg gatgcatata aaaaactgga gaaaaacgga
     3121 cttaacttac gaacggtctg tataaccgca aaagataaag tgtgtcccat ggataaaaaa
     3181 gactgtgatc cggagaaatg tcccagagca aaccactatt atataagagc aagggcatgc
     3241 ttgaaagaaa tacttcatga acagtttttt gaccgcgata taattgcccg gtatgccgat
     3301 gaattcaata tctgtccgtt tgagctgagt ctggatgttt ccggttactg cgacctcata
     3361 atatgcgact acaactatct tttcgatccc agagtccggt tgcaaaggta tttcgattat
     3421 gaaaatgagc agtctttctg ttttatggtg gatgaagcgc ataatcttgt ggagcgggga
     3481 agggagatgt tttcctgctc cataggtata aagcagctgg aggatttcaa agacagcatt
     3541 aaaaaagaat ggaaggcact gagaaaaagc gtaaatgcac tgattaacga aatgaaggag
     3601 ttgcaggccg aacatttttc gaccatgaac caaggtttta tttcacaggc agagttacct
     3661 ttggttctta cggacagggc taacagatgt gtcaatgaca ttatgatgta cgatctttcc
     3721 aaaatacccg acgataagag agaatctttc ttggaaggta tgtttaatat tctgttcttt
     3781 tcccgtgtcg cacaaatgta tgacagacgg tttaccacac tgtttacctc cgacggctta
     3841 gggctggatg tcaaactgat gtgtctggac ccttcctatc ttttgaagca aagcatggat
     3901 acgggaagat cggcaatatt gttctccgca acgctggagc ccaaaagata ttacatggat
     3961 cttttgggcg gtgacggcaa tatggacagt ttcctgacat tggattcgcc ctttccgaga
     4021 gaaaacttga aagtaaaact ggttacggac atatccacaa agtataaaga ccgggacctg
     4081 tcctatgaga aaatagcaca actgctgaaa aagcaattct ctcaaaagac cggtaactat
     4141 atggtatttt
//
LOCUS       DAOPWC010000089         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_21822_length_4124_cov_3.257557, whole genome shotgun sequence.
ACCESSION   DAOPWC010000089 DAOPWC010000000
VERSION     DAOPWC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21822_length_4124_cov_3.257557"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKJ65_02830"
     CDS             <1..615
                     /locus_tag="PKJ65_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ40805.1"
                     /translation="ITLENVGPKRAYHYYQIDYILTEIPDDCAYFFAQFRRENPVKYK
                     TDYTIIDNIKGKGHYVGTYMTWGANNNGWWGEGEIKFFMDGDDKFPTICGTGTEDYFC
                     GAYCFMENDRYLDYSTAYSGFYPVRYEKEAQGHQRFSMYRWHITDPIRFEKDLRVTMQ
                     ALGWRSNTKYLALQDDVSSVAYWYQTLPFNKFPTLPTYEFLEII"
     gene            615..1085
                     /locus_tag="PKJ65_02835"
     CDS             615..1085
                     /locus_tag="PKJ65_02835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNZ40806.1"
                     /translation="MITVCCYQSKIGRINISEDNEGICGVSICTSNECSHLSQSKLKD
                     ETFRQLAEYLQGKRKSFNVKLSLKGTEFQKSVWEQLLNIPYGETRSYGEIAALIGNAK
                     ASRAVGMACNRNPVMIIVPCHRIIGKKGCLTGYAAGIDLKEKLLDMERELKSNR"
     gene            complement(1117..2493)
                     /locus_tag="PKJ65_02840"
     CDS             complement(1117..2493)
                     /locus_tag="PKJ65_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40807.1"
                     /translation="MINEMIAECSDYDFKEILEVKRPQSWLKTVSAFANGTGGTLFFG
                     VDDTGAVVGLDDIKSHIGKITDMIQAKIEPNPVFHIIPCKENNLTFLKVDILSGQFTP
                     YYYYSDGNRIAYYRLGDESIQAPSYILNELILRGSGQTYDSVLTQELSSDYSFSYLKS
                     KYLNTVNLRFNSEDFVSFGLSDGKNLSRAGLLLADTNRVRQSRIFCTRWNGVDKLSEE
                     TVINDAEISGCLLIQLDRAIDFFKANTNIKWHKERGETVYNPDYDEGAVKEALVNAII
                     HRDYNVLGAEVVLNIYDDRLEITSPGGMCSGKEIPSVVDKVMESIRRNPIIADLFHRL
                     HLMNRRGSGLANITNRTNKLFDDGDNHVFYRSDNEFFVVKIENANYSVNVETINDRNE
                     TLNRNEAAVLKLIKKNSFITQNEMANALSLSRSTVIRITNKLTRNGIIQRVGANKNGY
                     WKVIRMYL"
     gene            complement(2727..3425)
                     /gene="araD"
                     /locus_tag="PKJ65_02845"
     CDS             complement(2727..3425)
                     /gene="araD"
                     /locus_tag="PKJ65_02845"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HNZ40808.1"
                     /translation="MNSLAQLKQRVYRANMDLVKHDLIVLTWGNASAIDREKELVVIK
                     PSGIEYDKLQPEDMVIVNMDGKVKEGSLKPSSDILTHIELYKAFPEIGGVVHTHSCFA
                     TAFAQAGKPVLPFGTTHADCFLGPIPCTRFLKDRELADYEKNTGKVIAETFTSLDYRV
                     IPAALVAGHGPFIWGRTPEESVLHAVTLEAVAKMAYLTITLGGEQCIGQKLLDKHYFR
                     KHGKDAYYGQEDNK"
     gene            complement(3418..>4124)
                     /locus_tag="PKJ65_02850"
     CDS             complement(3418..>4124)
                     /locus_tag="PKJ65_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ40809.1"
                     /translation="NPEIDIMSTPDGYPTAVIHCGNCTGDIDAWMHIFQEVLKATGSS
                     IEKSKLFETLMNKAMEADADLGGLMNFNYISGESITGIKPGVPLFLRQPGARFTLANF
                     LQTHLFSALATLRIGMEYFFKEGVKFNRIICHGGFFHSQAGAKAMAAALDSPVYLYKT
                     ASLGGPWGMALLAAYSQSEESLTLADYLDNKVFKNFELIQVLPDRDTVTSFVSFLEKY
                     RKFLKVEADASRCFNE"
BASE COUNT         1217 a          840 c          808 g         1259 t
ORIGIN      
        1 ataacactgg aaaatgtagg tccgaaacga gcatatcatt actatcagat tgactatata
       61 ctaactgaaa tacccgatga ctgtgcatat ttctttgctc agttcagaag agaaaatccc
      121 gttaaataca agaccgatta cactattatc gataatataa aaggcaaagg acactatgtc
      181 ggaacataca tgacatgggg agccaataac aacggatggt ggggagaagg ggagattaag
      241 ttctttatgg acggagacga taaattccct acaatctgcg gtaccggtac cgaagactat
      301 ttctgcggag catactgctt tatggaaaat gacaggtatc tggattattc caccgcctat
      361 agcggatttt atcccgtaag atatgaaaaa gaagcgcaag gtcatcagcg tttttccatg
      421 tacagatggc atataaccga ccctataaga tttgaaaaag acttaagggt taccatgcag
      481 gcgttaggtt ggagaagtaa caccaaatat cttgcactgc aggatgatgt ttcttcggtg
      541 gcttattggt accagaccct gccctttaat aaattcccga ctttaccgac ctacgagttt
      601 ttagagatta tataatgata accgtttgct gttaccaatc gaagataggc agaatcaata
      661 tatcggaaga taacgaaggt atatgcggtg tatctatttg cacatcaaat gagtgtagcc
      721 atttatcgca atcaaaactg aaagatgaaa cattcaggca acttgccgaa tatctgcaag
      781 gcaagcggaa aagtttcaat gtaaagcttt cactgaaggg caccgagttt cagaaatcgg
      841 tatgggagca actgctgaat attccgtacg gagaaacaag gagctacggt gaaattgcag
      901 cactcatcgg aaatgcaaaa gcaagcaggg cagtaggtat ggcatgcaac agaaatcctg
      961 taatgataat agtgccttgc cacaggatta taggcaaaaa aggttgttta acaggttatg
     1021 cagcgggaat tgacttgaaa gaaaagctat tggatatgga gagggaactc aaaagcaacc
     1081 gttaaattac ctaataaata catatttgtc tgttttttat aaatacattc taatcacttt
     1141 ccaataaccg tttttattag cgcccaccct ttgaattatc ccgttccgtg ttaatttatt
     1201 tgttatcctt ataactgtgc ttctggacag ggaaagtgca tttgccattt cattttgtgt
     1261 aatgaaagaa tttttcttga ttaatttaag aacagctgct tcattcctgt ttaaggtttc
     1321 attcctgtca tttatggttt caacattgac actgtaattg gcattttcaa ttttaacaac
     1381 aaaaaactcg ttatccgaac gataaaaaac atgattatca ccgtcatcaa aaagtttatt
     1441 ggttctgttt gttatgtttg caagaccgga tcctcttctg ttcataagat gtaatctgtg
     1501 aaataaatca gctataatag gatttcttcg tatggattcc attactttgt ccactacact
     1561 gggtatttct tttcctgagc acattcctcc gggagaggtt atttccaatc tgtcatcata
     1621 aatattcagc acaacttccg caccgagtac attgtaatct ctatggataa tagcattaac
     1681 taaagcttct tttaccgctc cttcatcata atcgggatta tagactgttt cacctctttc
     1741 tttatgccat ttgatgtttg tgtttgcctt gaaaaaatca atagcacgat ccaattgaat
     1801 cagaagacaa ccgcttattt cggcatcatt aataaccgtt tcttcactga gtttatcaac
     1861 cccgttccat ctggtgcaaa aaattctgct ctgtcttact ctattagtat ctgcaagcaa
     1921 aagacccgct cgacttaagt tcttcccgtc ggataatccg aaagagacga aatcctcgga
     1981 attgaaacgc aaattaacgg tatttaaata cttggattta agatagctga atgaatagtc
     2041 gctacttaat tcttgtgtta aaacagaatc ataagtttga ccggatcctc tgagtatcag
     2101 ttcgttgagg atatatgagg gtgcttggat tgattcatct cctaaacgat aatacgcaat
     2161 tctgtttccg tcactataat agtagtaggg agtaaattga ccgctcaaaa tatccacttt
     2221 caaaaaagtc agattattct ctttgcaggg aataatatga aaaacaggat tcggttcaat
     2281 ttttgcctga atcatgtccg ttattttccc gatatgactt tttatatcat ccaggccgac
     2341 aactgcaccg gtatcatcaa cgccgaaaaa caatgtcccg cctgttccgt tggcaaaagc
     2401 ggaaacggtt tttaaccagc tttgcggtct tttaacttcc aatatttctt tgaaatcata
     2461 atcactgcac tctgcaatca tttcattaat catgtgaacc ggccctctct ttatcaccga
     2521 tatatattca tatgatacta taatgatacc ataaatgcaa ccataatgac accataaatt
     2581 gtgcaattct ttaattgtat gatacatata gcagagtagc agtcaagtga caattactta
     2641 ttatgggatt ttaaccttta ccgttcttaa cctgttttaa acaccttctc aatagagatt
     2701 ccggttttat gagcagaaaa tatgtttcat ttattatctt cttgtccgta ataggcgtct
     2761 tttccgtgct ttctgaaata gtgtttatct aaaagtttct gtcctataca ttgctctccg
     2821 cccagggtaa tggtcagata agccattttt gccaccgctt caagtgtcac agcatgaagt
     2881 acgctttcct caggtgtcct gccccaaata aaagggccgt gtccggcaac caatgcagca
     2941 ggtattacac ggtaatccag agaagtaaat gtttccgcaa ttacttttcc ggtatttttc
     3001 tcgtaatccg caagttcacg gtctttcaaa aagcgggtac aggggatagg ccctaagaaa
     3061 cagtcggcat gagttgtgcc gaaaggtaag acaggttttc cggcctgtgc aaaagctgtc
     3121 gcaaaacagg aatgtgtatg gacaacaccg ccgatttcag gaaatgcttt gtataactct
     3181 atatgagtca gtatgtcgct cgagggcttg agactgcctt ctttaacctt gccgtccata
     3241 tttactatga ccatatcttc cggttgcagt ttatcatact ctattccgct gggtttaatg
     3301 acaaccagct ctttttctct gtctatcgca ctggcattgc cccatgtcag gacaataaga
     3361 tcgtgtttga ctaaatccat attggcacga taaactcttt gttttaattg tgctaggcta
     3421 ttcattaaag cacctcgatg catctgcctc tactttgagg aattttctgt atttttctaa
     3481 aaaagacaca aagcttgtga ccgtatcccg gtcgggaagc acttgaatca attcgaagtt
     3541 tttgaatact ttattatcta agtaatcggc aagtgtgaga ctctcttcag attgtgaata
     3601 ggcagccagt aaagccatgc cccagggtcc gcccagactt gcggttttat acaggtaaac
     3661 aggagaatca agtgcagcag ccattgcttt ggctcccgcc tgtgagtgga aaaaacctcc
     3721 gtggcagatt attctgttaa acttcacacc ttctttgaaa aagtattcca tacctatcct
     3781 gagtgtggca agagcggaaa agagatgtgt ctgcaggaag tttgcgaggg taaaccttgc
     3841 tcccggttgt cttagaaaaa gcggtactcc gggtttaatt cctgtaatgc tttcaccgga
     3901 gatataattg aaattcatca aacctccgag atcggcatca gcttccatgg ctttattcat
     3961 cagagtctca aagagcttgg atttttcaat ggaagaaccg gttgctttga gaacctcttg
     4021 aaatatgtgc atccatgcat cgatatcgcc ggtacagttg ccgcaatgaa tcacggcggt
     4081 gggatacccg tccggagtgg acattatatc aatctcagga ttgt
//
LOCUS       DAOPWC010000090         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_22012_length_4097_cov_2.864671, whole genome shotgun sequence.
ACCESSION   DAOPWC010000090 DAOPWC010000000
VERSION     DAOPWC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22012_length_4097_cov_2.864671"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKJ65_02855"
     CDS             <1..315
                     /locus_tag="PKJ65_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40810.1"
                     /translation="GCIDQLIPMWLENGVNTMFPIEVGVWNASIAPWREQYGKELRGV
                     GGMNKNVLSYDKKAVDDEIERLKPLIALGGYIPCPDHRLPPRTKYELVQYYAEKIKTV
                     KI"
     gene            315..1367
                     /locus_tag="PKJ65_02860"
     CDS             315..1367
                     /locus_tag="PKJ65_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40811.1"
                     /translation="MERIIKKQNKLYPESEMADWFKLFYQSEFGNAHIPEDRNSNFIA
                     LKDEIEDKKSNKADIEDIGNGNVRVHLGHLKKIGLSQELFVEIMMQSRLLSKGTREGF
                     SDKVKALEKAVKESSLPITEEEFYTFKESYYAAGCRLISHSERYKEAYNPSYRVIRAE
                     YANKMKLWAKVEKLQNEAKINGTTVNIAIDGKSSAGKTYLADMFSGIFDCNIIHMDDF
                     FLQNHQRTIERLLQPGGNVDYERFQKEVIPNLKSPVEFTHHTYDCQTNVISEKKICAK
                     TLNIIEGAYSMHPYFGDIYDLCIFMDIDKETQFKRILKRNGELYKRFTNRWIPLENEY
                     IENFKIKERCDFIFKV"
     gene            complement(1391..2527)
                     /locus_tag="PKJ65_02865"
     CDS             complement(1391..2527)
                     /locus_tag="PKJ65_02865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40812.1"
                     /translation="MISTRMCTYKDKAAMKKLWTELFGDSKSFTDWFFDNRFYPQYCV
                     CTYDNDLLVSCAHSLPMPVLIRGKEIPCAMISGIATVEAYRKQGLMHKTLLYLINELN
                     AKDIYAATLKAVNLSTYYSMHYYGCTLSAYIRSGKKDKYACLMPYIEEKANSDVSCRQ
                     IDIYDEFDGLHACYTGFIKGYSGIAIRDKAAFRLKMNDYLSDNAKAIAVFKDGKITGY
                     CIYFDEKDLYAEEYITDDLYSACAMFKALKEQGKSITVKTSKKVADRVGVKNTEVTEQ
                     NSMGIINIGKLLESICGNGDYGVKVFDSFYNPNNGIYLLNGKKVNDKYHLELSSGHLM
                     QLLTGHKSLEELVSEGVAVIHDKVACDEISKMFPEQNCFIADEY"
     gene            2609..3010
                     /locus_tag="PKJ65_02870"
     CDS             2609..3010
                     /locus_tag="PKJ65_02870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNZ40813.1"
                     /translation="MKTEIKVYYIYEYMQKGMITMCNQAQALQILKEVYEACKPVFSD
                     TLKEIYLYGSYARGDYHNESDIDILIVLDLEASEISAYRRTIAAIASDLSLKHDITIS
                     VTMKPVVQFRKYANVLPFYKNVIEEGIRYGA"
     gene            3000..3395
                     /locus_tag="PKJ65_02875"
     CDS             3000..3395
                     /locus_tag="PKJ65_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNZ40814.1"
                     /translation="MELKERKSLSDVRYQRALECLAAAKSLLESGNYKSAANRSYYAV
                     FHAMRSVLAFDEVDRRHHSSVISEFRKRYIKTGIFETELSKIISVLFDTRTDSDYDDF
                     FVISKEEVVEQTKNAEYFLNQIKLFLDKK"
     regulatory      3514..3609
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3648..>4097
                     /locus_tag="PKJ65_02880"
     CDS             3648..>4097
                     /locus_tag="PKJ65_02880"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HNZ40815.1"
                     /translation="MDLFLTQSNGNYLLTTYGYIAIALLAVLVFCAIYFFRPKKEKSE
                     LQVNPTKKLAFCSMAVAAGFVASYIRLFSMPLGGSVTLLSMFFICLAGYWYGTGTGLI
                     AAFTYGLLQLVQEPYIINFWQVCFDYLFAFTALGLSGLFKDKKNGLLT"
BASE COUNT         1270 a          747 c          860 g         1220 t
ORIGIN      
        1 ggatgtatcg atcagctgat acctatgtgg ctggaaaacg gagtcaacac catgttcccg
       61 attgaggtgg gtgtctggaa tgccagcatt gctccctgga gagagcaata cggtaaagaa
      121 ttaagaggag tgggcggaat gaacaagaac gtcctgtcat atgataagaa ggctgtggat
      181 gacgagattg agaggttgaa gcccctgata gccctgggcg gatacattcc ctgtcccgat
      241 cacaggttac cgccccgtac aaaatatgag ttggtacagt attacgcgga gaagataaag
      301 actgtaaaga tataatggaa agaattatta aaaaacagaa taaactatat cctgaatcgg
      361 aaatggccga ctggttcaaa ttattctatc agagcgagtt cggtaatgct catatacccg
      421 aagacaggaa cagcaatttt attgctctta aagacgaaat agaggacaaa aaaagcaata
      481 aagccgatat tgaagatatc ggcaacggca atgtgagggt gcacttgggt catcttaaaa
      541 aaatcggtct ttcacaggag ttgtttgtgg aaataatgat gcaaagcaga ttgctgtcta
      601 agggtactcg ggagggattt tccgataaag tcaaagcttt ggagaaagcg gtaaaggagt
      661 catcccttcc cataactgaa gaagagtttt atacatttaa ggaaagttat tatgcggcag
      721 gatgccgttt aataagtcac agtgagaggt ataaagaggc atacaatcct tcttacaggg
      781 taatacgtgc ggaatatgca aataagatga aattatgggc aaaagtcgag aaacttcaaa
      841 acgaagcgaa aataaacgga acaacggtga atattgccat tgacggcaag agctcagccg
      901 gcaagacata tcttgccgac atgttttccg gaatatttga ctgtaacatt attcatatgg
      961 atgatttctt tttgcaaaac catcaaagaa caattgaaag gctgctgcag ccgggcggaa
     1021 atgtcgatta tgagcgattc caaaaggaag tgatacctaa ccttaaatcg cctgtggaat
     1081 ttacccatca tacatacgac tgtcagacca atgtaataag tgagaaaaag atatgtgcaa
     1141 agacactgaa tataatcgag ggcgcttaca gtatgcaccc gtatttcggc gacatatatg
     1201 atttatgtat attcatggat atcgacaagg agacgcagtt taagagaata ctgaaaagaa
     1261 acggcgaact gtacaaacgt tttaccaaca gatggattcc tttggagaat gaatatattg
     1321 aaaactttaa gataaaagaa cgatgcgact ttatcttcaa agtgtagtct ttaaataaat
     1381 agtatacggt ttaatattcg tctgcaataa agcaattctg ttcgggaaac atttttgata
     1441 tttcgtcaca tgcaacttta tcatgtataa cggcgacacc ctccgacacc agctcttcca
     1501 atgacttatg ccccgtaagt aactgcataa gatgtcccga agacagctcc agatggtatt
     1561 tatcattaac tttcttaccg tttaaaagat aaataccgtt gttggggtta tagaaactgt
     1621 caaaaacctt tacaccgtaa tcgccgttac cgcagatgct ctccaacagt ttaccgatat
     1681 taattatacc catggagttt tgttcggtaa cttccgtatt cttaacccct acccgatccg
     1741 caactttctt tgaagtcttt accgttatac tcttcccctg ttctttaagt gccttaaaca
     1801 tagcgcatgc gctgtataaa tcgtcggtta tatattcttc cgcatataaa tctttttcat
     1861 caaagtaaat acagtatccc gttatttttc cgtctttaaa cacggcaatt gctttggcat
     1921 tatcgcttaa gtaatcattc attttcaacc tgaatgccgc cttatctctt attgcaatac
     1981 cgctgtatcc ctttataaat cccgtgtaac aggcatgtaa accgtcaaac tcatcgtata
     2041 tatctatttg cctgcatgaa acatcgctgt ttgctttttc ttcaatatag ggcattaaac
     2101 aggcatattt atctttctta cccgaacgga tatatgcact taaagtacaa ccgtagtagt
     2161 gcatcgaata atatgttgaa agatttaccg ctttcaatgt ggctgcataa atgtcttttg
     2221 catttaactc gtttattaaa tatagcaaag tcttatgcat gagaccttgt tttctgtatg
     2281 cttcaacggt ggctattccg gatatcatgg cgcagggtat ttctttacct ctgattaaaa
     2341 caggcatggg aagtgaatga gcgcacgata ccaataagtc attgtcataa gtgcatacgc
     2401 agtattgagg ataaaatctg ttatcgaaga accagtcggt gaaacttttg ctgtcaccga
     2461 aaagctcagt ccagagcttc ttcatagccg ctttatcttt atatgtgcac atccttgttg
     2521 aaatcattgt tacctcttct aaattatata tcaaaaaaga tgattaccca atgcttaata
     2581 aagcgaatcg gtttttttgc gggattgatt gaaaactgaa ataaaggtat actatattta
     2641 cgaatatatg cagaaaggaa tgattactat gtgtaatcaa gctcaagcac ttcaaatcct
     2701 gaaagaagta tatgaagctt gtaagcctgt tttcagcgac acattaaaag aaatctattt
     2761 atacggttct tatgcaagag gcgattatca caatgagtct gatatcgata tccttattgt
     2821 tttagacctg gaagcttcgg agatttcagc atatagaaga acaattgcag caattgcaag
     2881 cgatttgagt ttgaaacacg atatcacaat ttccgtgacg atgaagcctg tagtacaatt
     2941 tcggaaatat gcgaatgtgt tgccttttta taaaaatgta attgaggaag ggattcggta
     3001 tggagcttaa ggagagaaaa agtctttccg atgttcgcta tcaacgtgct ttggaatgct
     3061 tggctgctgc gaagtctttg ttggaaagcg gtaattacaa aagtgcagct aatcgttctt
     3121 actatgcagt atttcatgcc atgagatccg tgttggcgtt tgatgaagtg gatagaagac
     3181 atcacagtag tgttatttca gagttccgta agcgctatat taaaacaggc atatttgaaa
     3241 cagagctatc caagattatt tccgtactgt tcgataccag gacagatagt gattatgatg
     3301 atttctttgt tatttccaag gaagaggttg ttgaacagac gaagaatgcc gaatattttt
     3361 taaatcagat taaattgttt ttggacaaaa agtaaaagtt gatgtcaagc tgatacaatc
     3421 agtaaataga gataggtaat tatcattcgc aagtcataat atgcttgaca ttaatactta
     3481 ctcgtaatag aatcaacttc accgagaaat cggcatatat gctaggggag ccatgtggct
     3541 gagagatatt attgaccctc attacttgat ccgggtaata ccggcgtaag gaagcaaccg
     3601 acagtactgt ttcacctcct gcatgtataa acaagggagg tatttttatg gatttgtttt
     3661 taactcaatc gaacggcaat tatctgctga caacatacgg atatatcgca atagcgttgt
     3721 tggctgtctt ggtattttgt gcgatttact ttttccggcc caagaaggaa aaatccgaac
     3781 tacaggtaaa tcctacaaag aaactggcct tttgttccat ggcggttgca gcgggctttg
     3841 tggcatcgta cataaggttg ttcagtatgc ctttgggcgg ttcggtaacg ctgttatcca
     3901 tgtttttcat atgccttgca ggttactggt acggaaccgg taccggtctt attgccgctt
     3961 tcacatacgg tcttctgcag ttagtgcagg aaccgtatat cataaacttc tggcaggtgt
     4021 gttttgatta cctgtttgct tttaccgcac tggggttgtc gggacttttt aaagataaaa
     4081 agaacggtct tttaaca
//
LOCUS       DAOPWC010000091         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_22165_length_4077_cov_2.555445, whole genome shotgun sequence.
ACCESSION   DAOPWC010000091 DAOPWC010000000
VERSION     DAOPWC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22165_length_4077_cov_2.555445"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1084
                     /locus_tag="PKJ65_02885"
     CDS             173..1084
                     /locus_tag="PKJ65_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40816.1"
                     /translation="MNKIKLYVKSSDNEYAGRLKEYIKINGLNMFILTEDNADVVLED
                     SGAENELLLKTSSKEWHTGKYQSADKIISAIMENIDISAGINVKSENRTIFVTSAHGG
                     AGKTFISQALSIYLSRNGRKVLYINLDGLCVKDSVFRCEQQNDISLIAYYASKGNENI
                     NTCIERYKNHDVTKNVYFLNSIYPSYDVTFDMSSAKVFINSLNANSIYNYTIIDCPLY
                     PISPYAFIMKNAYKTLFIKCRGSEREEEVAAFLQKNDIEVIQLCNMIRYTEGIYIPKA
                     EEDIAHNLPDFYEAIEQVVYELEKNDE"
     gene            1059..2216
                     /locus_tag="PKJ65_02890"
     CDS             1059..2216
                     /locus_tag="PKJ65_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ40817.1"
                     /translation="MNWRKMMNNDYDKIIKKCLEGITGMEEGEIEKTVTEYVYDIKNK
                     DMENKPEVIKRVMDAITGYGILQPLMEKEGITEIMVNADRIFYEEKGVIHRYNISFTK
                     QDIDKLISILCRKTDRTVNMANPILDGSIGHDIRVNIILEPISHNGHSITVRRFPEKQ
                     ITYEDMIRDGFFTKEISDFLDNAVKERLNIFICGGAGCGKTTLLNILTGFVPNSERVI
                     TIEDSVELMIDRIDNIIRLTCRNPNNEGKGEISMDSLIRASLRMRPDRIIVGEIRGKE
                     ALYMLQAMNTGHIGSISTGHANSCKDAFSRIETMVLMECDMPFLAIRKQIYDAIDIII
                     SLQRIDGKRVLTEIVQVTEFEDGEIKTECLFTYDIESGKHIKVGEATRRRK"
     gene            2213..2923
                     /locus_tag="PKJ65_02895"
     CDS             2213..2923
                     /locus_tag="PKJ65_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40818.1"
                     /translation="MRFEFIKRAVTTGLVYAICVYFLYLSLLVSAITGILVAGICLLD
                     KKYMKETIQKRNRDGFLRFLEILLSYTAIPSDFIHAFSKALLEFKRIYQRDYMLTLVD
                     NVYSKTMANMSTDSILQSFAEHLDIEEAYLFCDSIIICEKKGADLNRTIKETIAFIKE
                     KRSIEQEIEVITEEKKTERFIVSLCPFFILALFNTMGTGYLDILYEGLLGRAIMTAAG
                     ILFLISSYLAKKILEVKV"
     gene            2920..3744
                     /locus_tag="PKJ65_02900"
     CDS             2920..3744
                     /locus_tag="PKJ65_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ40819.1"
                     /translation="MILFAVYVLLILFTLLYSRKVRFDFPLCHCTFMKATYKAFVPLA
                     KVAARYTASDNRLDLLYPKTAKEDLHIADTCLKMNIFVIMLFLPTIAIACKASAVVTA
                     ISVLLCFASVKCVDFDISKVYENKKQEIITDFSVFLTKLTLCLGAGMTLYQAFTRQDY
                     DLPDRTFKAHLDRLINNLTMHSDPEYSFLELSLMLPIPQINAFCSIMITGFKNTEAGI
                     KDTLKTYTSSIWTERRNNAKKRAEQSSAKAVLALAIGLIGILLVLTAPAMIMLMSI"
     gene            3787..3960
                     /locus_tag="PKJ65_02905"
     CDS             3787..3960
                     /locus_tag="PKJ65_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HNZ40820.1"
                     /translation="MRIENGKKGMGTVEVIIILAVLVGIALIFREAITNFVQGIIDGT
                     LNNQGILEKLRVK"
BASE COUNT         1369 a          651 c          898 g         1159 t
ORIGIN      
        1 cagttttatc cctccgattt caggaaaaca gagttttctt tatttacttt atcaacataa
       61 gcagagtcat aaaaaagaat gactgtctat ttatactatg ttacatgtgt ttgtcatatg
      121 ttgaatgaga attaatggta aataatggta taattaaccg gcaggagtta atatgaataa
      181 gataaaacta tatgttaagt catcggataa tgaatatgcc ggacggctga aagaatacat
      241 taaaattaac gggttgaata tgttcattct gactgaagat aatgcggatg ttgttcttga
      301 agattccggt gcagagaatg aacttctttt gaagacatct tctaaagaat ggcatacagg
      361 taagtatcag tccgcagata agataatcag tgcaatcatg gagaatattg atatatccgc
      421 cggtataaat gtaaagtcgg agaacaggac gatatttgtg acatcggctc acggcggtgc
      481 gggtaagaca ttcatttcac aagctctgtc aatatattta tcccgaaacg gcaggaaggt
      541 actgtatatc aatcttgacg gattatgcgt aaaagattcg gttttcagat gtgaacagca
      601 gaatgatatt tcgctcatag catactatgc ttctaagggt aatgagaata tcaatacatg
      661 tattgaaagg tacaaaaacc atgatgttac aaagaatgtg tacttcctga acagcatata
      721 tccttcatat gatgtaacct ttgacatgag cagtgcaaaa gttttcatta acagtctcaa
      781 tgcaaatagt atatataact acactataat cgactgtccg ttatatccaa tcagtccgta
      841 tgcctttatc atgaaaaatg catacaagac actgtttatt aaatgcagag gcagtgaaag
      901 agaagaagag gttgcggctt ttttgcaaaa gaatgatata gaggttattc agttatgcaa
      961 tatgatccgg tataccgaag gaatatatat acccaaagcc gaagaagata tagctcataa
     1021 tctgccggat ttctatgaag caatcgaaca ggttgtgtat gaactggaga aaaatgatga
     1081 ataacgatta cgacaaaatc attaaaaagt gtctggaagg tataaccggt atggaggagg
     1141 gggaaattga aaaaaccgtt accgaatatg tgtatgatat aaaaaataaa gatatggaga
     1201 ataagccgga agttataaaa agggtaatgg atgccataac gggatacggg atattacagc
     1261 ctctgatgga aaaagaaggc attacggaaa taatggtaaa tgccgacaga atattttacg
     1321 aggaaaaggg tgttattcat cgatataata ttagttttac aaaacaggat atagataaac
     1381 tgatatcgat actgtgcaga aagaccgaca gaaccgtaaa catggccaat cccatattgg
     1441 acggcagtat agggcatgat atacgggtta atatcatatt ggagcctatc tcccataacg
     1501 gtcacagtat aaccgtaaga agatttccgg aaaaacagat aacttatgag gatatgataa
     1561 gggacggctt tttcacaaaa gaaatttcgg attttctgga caatgcggta aaagagcggc
     1621 tgaacatatt tatatgcggg ggagcgggat gcggtaaaac gacattattg aacatattga
     1681 caggatttgt tcccaacagt gaaagagtga taacaattga ggattcggta gaacttatga
     1741 ttgaccgtat agacaacata attcgtttaa catgccgtaa tcccaacaat gagggaaaag
     1801 gcgagataag catggacagc ctcatacgtg caagtttaag aatgagaccg gacaggatta
     1861 tagtggggga gataagaggt aaagaagctt tatacatgct gcaggctatg aacaccggtc
     1921 atataggttc catatccaca gggcatgcaa attcctgtaa agatgcattt tcccgcatag
     1981 aaaccatggt actgatggaa tgtgatatgc cttttttggc tatacgaaag cagatttatg
     2041 atgccatcga cattattatc tcgctgcaaa ggattgacgg aaagagggtg cttaccgaaa
     2101 ttgtacaggt tacggagttt gaagacggtg agattaaaac cgagtgcttg tttacctatg
     2161 atattgaatc gggaaagcat atcaaagtcg gggaagcaac aaggagacgg aaatgagatt
     2221 tgagtttata aaaagggcgg taacaaccgg tcttgtatat gcaatatgcg tctattttct
     2281 ctatctgtct cttcttgtat cggcaataac gggaatactt gttgcgggaa tatgtcttct
     2341 tgataaaaaa tacatgaaag aaacgattca aaagaggaac agggacggtt ttttaagatt
     2401 tttggagatt ctgctgtctt acactgccat accttcggat tttattcatg cgttttccaa
     2461 agcacttctg gagtttaaaa gaatatatca aagagattat atgcttacat tagtcgacaa
     2521 tgtttactca aaaacaatgg caaatatgtc taccgacagt attttgcaat ctttcgcaga
     2581 gcatctggat attgaagaag catacctgtt ttgtgacagt ataatcattt gtgagaaaaa
     2641 aggtgcggat ttgaacagga ccataaagga gaccattgct tttataaaag aaaaaagaag
     2701 tatagaacag gagatagaag taatcaccga agaaaagaaa accgagcggt ttattgtgag
     2761 cctgtgtccc ttctttatac ttgccctttt caataccatg gggaccggat atctggatat
     2821 tctgtatgaa ggtcttttgg gcagagctat aatgactgcg gcgggcatac tgttcttaat
     2881 atcttcatat cttgcaaaaa agatactgga ggtaaaggta tgatactttt tgcggtttat
     2941 gttttattaa tactctttac cttgctttat tccaggaaag tgcggtttga ttttcctttg
     3001 tgtcattgta ctttcatgaa ggcgacatat aaggcatttg ttccgttggc aaaagtcgct
     3061 gcaagatata ccgcttcgga taaccgactg gatttgctgt atcctaagac agcaaaagaa
     3121 gatttacata ttgccgatac ctgtttaaaa atgaacatat tcgttattat gctttttctt
     3181 ccgacaattg caattgcatg taaagcatct gcagtggtta ccgctatatc ggtattgctt
     3241 tgtttcgcaa gtgtaaagtg tgtggatttc gacatatcta aagtgtatga aaataaaaag
     3301 caggagatta ttacggattt ttcggtattc ttaaccaagc tgacattgtg tctaggagcg
     3361 ggtatgactc tttatcaggc ttttacccga caggattatg accttccgga tagaacattc
     3421 aaggcgcatc tggaccgcct cataaacaat cttacaatgc attccgaccc ggaatactcc
     3481 tttctggaat tatcccttat gcttcccata ccgcagatta atgctttttg ttccataatg
     3541 ataaccggtt tcaagaatac cgaagcggga ataaaagata ctctaaaaac atatacttca
     3601 tctatatgga cggaaagaag aaacaatgcc aaaaaaagag cggagcaatc gtctgcaaaa
     3661 gcggtattgg ctctggcaat agggctgata ggaatactgt tggttcttac cgctcccgcg
     3721 atgattatgc ttatgagtat ataggataac cataaaatca ctaccatata ggtgaaggag
     3781 gataatatga gaatagaaaa cggaaaaaaa ggaatgggta cggtggaagt gataataata
     3841 cttgccgttc tggtaggtat agcgttaatc ttccgggaag ccataacaaa ctttgtgcag
     3901 gggataattg acggtacatt gaataatcag ggtattttgg aaaagttaag ggttaaatga
     3961 catgataagc gagataatac agagcaacca agacaggatg ttgcttgtca gcggtgacaa
     4021 aatattgtgt cccgaccata tgatacagat gctgcaacat tgctttattg attgctt
//
LOCUS       DAOPWC010000092         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_22198_length_4072_cov_3.409759, whole genome shotgun sequence.
ACCESSION   DAOPWC010000092 DAOPWC010000000
VERSION     DAOPWC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_4072_cov_3.409759"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..825)
                     /locus_tag="PKJ65_02910"
     CDS             complement(34..825)
                     /locus_tag="PKJ65_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNZ40821.1"
                     /translation="MGIIENIRQFFLQTPSNFWVFILVGFIAQVIDGTLGMAYGVSCN
                     SFLLTFGVSPAIASASVHIAEVFTTSVSGFSHWRLKNVDKSMFFKLLIPGVIGGAVGA
                     YVLSNFEGGTIKPFINAYLIVMGVVIIVKIFKKQKEKHLGGWIYPLGLAGGVLDAIGG
                     GGWGPVVTSTLIATGVEPRYTIGTVNAAEFFVTLAESIMFISFLGLQDSVYAILGLVI
                     GGIIAAPFGAFLCKRVPMKPLMALVGALVIFLNVRSLIKFIVGLL"
     gene            complement(830..2620)
                     /locus_tag="PKJ65_02915"
     CDS             complement(830..2620)
                     /locus_tag="PKJ65_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ40822.1"
                     /translation="MTENSNEHMNIVIVGHVDHGKSTVIGRLLADTGSLPEGKLEQIK
                     ELCRRTSKPFEYAFLLDALKDERSQGITIDTARSFFKTDKRKYIIIDAPGHIEFLKNM
                     ITGASRADAALLVIDAAEGVKENSRRHGYMLSMLGIKQVCVLVNKMDNVSYSEKAYSK
                     IVSEYDDFLKQIGIAAEGFIPVSAMQGDNIAKISINMPWYKGDTVLTMLDKFRATKPL
                     DSLPFRMPVQDVYKFTADNDDRRIIAGTVESGIFKQGDEVVFYPSGKKTHVKKIEPMS
                     EQEEVGKASAGYATGFTMTEQIYVRRGELCVKADELPPLMGKHMKVSLFWLGKNPMVK
                     DKKYYLKTGTMKAECYLEEILSVMDASTLVKTEKQYIDRHDVADCIISTSRLCAFDNV
                     AALASTGRFVIVDDYEISGGGIFTDTVENLHRYERAKVMLREGKWEHGYVTVEERSDK
                     HDQNPALIILTGAKDTNKKLFAKALERTLFDEGRNVYYLGIGSVIYGLSADINPPGEK
                     ESRSGENIRRLAEVANILTDTGLILIVTASDLTKYDIETINLVTKNDNMLVIWLGEEC
                     TTDIECDMVLRADEDALEIIRKRVEQITAR"
     gene            complement(2621..3502)
                     /gene="cysD"
                     /locus_tag="PKJ65_02920"
     CDS             complement(2621..3502)
                     /gene="cysD"
                     /locus_tag="PKJ65_02920"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNZ40823.1"
                     /translation="MDHLDRLEAKSIHIMREAYSCFKNLCMLWSIGKDSTVLLCLARK
                     AFYGHVPFPLVHIDTRYKIPSMVEYRDKLALEWNLDMIYGENTLALQEKRTFPDGNCT
                     RLECCKNLKTEALINTLSGEWTRYRLDHKLKKYVPDPNTEPFTGVIVGVRADEEGSRS
                     KERYFSPRDTHSDWDISDQPPEFWGQFKTDFAPGTHLRIHPLLDWTELDIWEYIKRER
                     VPIVPLYFDQGNNTRYRSLGCYPCTKPVASSAKNIDDIIKELKTGKFANIAERSGRDQ
                     DKEDGGGLEELRKGGYM"
     gene            complement(3502..>4072)
                     /gene="cysC"
                     /locus_tag="PKJ65_02925"
     CDS             complement(3502..>4072)
                     /gene="cysC"
                     /locus_tag="PKJ65_02925"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNZ40824.1"
                     /translation="NVVWHRNLDSKYRQEKTGNKGMVIWFTGLSASGKSTIASLVEKN
                     LIDSGKTAYLLDGDNVRHGLCSDLAFSERDRVENIRRLAEVAALFEDSGVIAIVSAIT
                     PMEKMRKLARSKAKHFVLVYVKADLDTCIERDPKGLYEKALKGEIKGYTGIDSPFEQP
                     EQYEILLDTDKYDVLTCANKVTEYIQGVL"
BASE COUNT         1041 a         1049 c          723 g         1259 t
ORIGIN      
        1 atgaaatcac ctttactcca gttcttaaac atattacagc aaccccacaa taaacttaat
       61 cagcgacctg acattgagga atataaccag ggcacctaca agcgccatca aaggcttcat
      121 aggcactctt ttacatagaa atgctccgaa cggtgccgct atgattcctc ctatgaccaa
      181 tcccaagata gcatatacgc tgtcttgcaa gcccaaaaaa gaaataaaca tgattgattc
      241 ggccaatgtc acgaaaaact ccgcggcatt aaccgttccg atggtatagc gcggttccac
      301 acctgtcgct atgagggtgg aagttactac cggcccccat ccgcctccgc ctatggcatc
      361 cagtactcct cctgcaagac caagaggata tatccatcct cctaaatgct tttctttctg
      421 ttttttgaat atcttaacta ttattacaac acccattact attaaatagg cattgatgaa
      481 gggttttatt gttccgcctt caaaattgga taacacatat gcaccgacgg ctcctcctat
      541 cacaccggga atgagaagct taaaaaacat gcttttatcc acattcttga gtctccaatg
      601 agaaaatccc gaaaccgatg tggtaaacac ttcggcaata tgcacgcttg ccgaagctat
      661 tgcaggagac accccgaaag tgagtaagaa tgaattacac gatactccgt atgccattcc
      721 caatgttccg tcaataacct gagcgataaa acctaccagt atgaataccc agaaatttga
      781 gggtgtctgt aaaaagaatt gcctaatgtt ttctattatc cccataaaat caacgtgcag
      841 ttatctgctc aaccctcttc cttataatct ccaacgcatc ttcatcagcc cgtaaaacca
      901 tatcacactc tatatcggta gtacattcct cgcccagcca gattaccagc atattatcat
      961 tctttgttac aagatttatc gtttctatgt catatttggt cagatcggaa gcggttacta
     1021 tgagaataag tccggtatcg gtaagtatat tggcaacctc ggccagtctt cggatatttt
     1081 cgcccgatct gctttccttt tctcccggag gatttatatc cgcactgagc ccgtatatca
     1141 cacttccgat acctaaataa tatacattcc tgccttcatc gaaaagcgtt ctttccagtg
     1201 ctttggcaaa cagctttttg tttgtgtctt ttgctccggt aagtattatg agggcgggat
     1261 tctgatcatg tttgtcgctt ctttcctcaa cggtcacata accgtgctcc catttgcctt
     1321 ctctcaacat gactttggct ctttcatacc tgtgaaggtt ctccaccgta tcggtaaata
     1381 taccgccgcc ggaaatctca taatcatcca ctatgacaaa ccgtccggtc gatgccaaag
     1441 ccgcaacatt gtcaaatgca cacagtctgg aggtagagat aatgcagtcg gccacatcat
     1501 gtctgtcaat atattgtttt tccgttttca caagagtgga tgcgtccata acgctcaata
     1561 tctcttccag atagcattcc gctttcatgg taccggtctt gaggtagtat tttttatctt
     1621 ttaccatggg atttttaccc agccagaaca gactgacttt catgtgtttt cccatcagcg
     1681 gcggcagttc atccgctttt acgcacaact cccctcttct tacatatatc tgttcggtca
     1741 tggtaaaacc tgtggcatat ccggcacttg cctttccgac ttcttcctgc tcgctcatag
     1801 gctcaatctt tttaacatgt gttttctttc ccgacggata gaataccacc tcatctccct
     1861 gtttgaatat cccgctttcc accgttcccg caataattct tctgtcatcg ttatcggcgg
     1921 taaacttgta tacatcctgt acaggcattc gaaaaggtaa cgaatccaaa ggtttcgtag
     1981 ctcggaattt gtccagcatg gtaagtaccg tatcgccttt ataccatggc atattgatac
     2041 ttatcttcgc aatattatcg ccctgcattg cacttaccgg gataaatcct tccgctgcaa
     2101 tgcctatctg tttaagaaaa tcatcatatt ccgaaacaat cttcgagtat gctttctcgc
     2161 tgtatgacac attatccatc ttattaacca gtacgcacac ctgctttata cccaacatgg
     2221 aaagcatata tccgtgtctt ctggagtttt ccttgacacc ctcggcagca tctataacta
     2281 aaagtgcggc atcggctctg gaagctccgg taatcatgtt tttaagaaac tctatatgtc
     2341 cgggtgcatc aataattatg tattttctct tatccgtttt aaaaaaactt cttgcggtat
     2401 ctatggtaat tccctgtgac cgctcatctt ttaatgcgtc cagtaaaaat gcatattcaa
     2461 aaggtttgct tgttcttctg cacagttctt taatctgctc cagttttcct tcgggcagag
     2521 agcctgtatc ggccaataat cgccctataa cggtgctttt gccgtggtct acatgcccta
     2581 caatgactat attcatatgt tcattgctgt tttcagtcat ttacatgtac ccgccttttc
     2641 tcagctcttc cagaccgccg ccgtcttctt tatcctggtc tcttcccgaa cgttcggcta
     2701 tattggcaaa cttaccggtt ttaagctcct taatgatatc atcgatgttt ttagccgatg
     2761 atgctaccgg ttttgtacag ggataacagc ccaaagaacg gtatctggta ttatttccct
     2821 gatcaaagta caaaggcaca ataggcaccc tctccctctt tatatactcc catatatcca
     2881 gttccgtcca gtccagcaaa ggatgtattc tcaaatgggt accgggagcg aaatccgtct
     2941 tgaactgccc ccagaactcc ggaggctgat cggatatatc ccagtcactg tgagtatctc
     3001 tgggagaaaa atatcgttct ttggaacggc tgccctcttc gtccgcccgc actcccacta
     3061 ttacccctgt aaacggttcg gtattggggt cgggtacata ttttttcagt ttatgatcta
     3121 atctgtaacg tgtccactct ccggataaag tatttatcaa cgcttcggtc tttaagtttt
     3181 tacaacattc cagtctggtg cagtttccgt cgggaaaagt tcttttttcc tgcaaagcta
     3241 acgtattctc accgtatatc atgtctaagt tccattccag cgccaattta tcccggtatt
     3301 ccaccatgga cggtatttta taacgggtat ctatatgaac cagcggaaag ggcacatggc
     3361 cgtaaaaagc ctttcttgca aggcataaaa gcacggtgct gtctttacct atcgaccaca
     3421 gcatacataa gtttttaaag caactgtatg cctcccgcat tatatgtata ctcttcgctt
     3481 ccagcctgtc cagatgatcc attacagtac tccttgtata tattccgtta ctttatttgc
     3541 acatgtcaaa acatcatatt tatctgtatc caataaaatc tcatattgct ccggctgctc
     3601 aaacggtgaa tcgatgccgg tatatccttt tatctcgcct ttcaaagctt tttcgtacag
     3661 tcctttggga tcgcgttcga tacaggtatc cagatcggct ttgacatata ccaaaacaaa
     3721 gtgttttgcc ttgcttcttg caagctttct catcttttcc atgggagtaa tggcgctgac
     3781 tatggctata actccgctgt cttcaaacaa agcagccacc tctgccaatc ttcggatatt
     3841 ttccaccctg tcacgctcgg aaaaagccaa atcggaacac aaaccgtgac ggacattatc
     3901 tccgtcaagc aaatatgccg ttttacccga atctataagg ttcttttcca ccaacgaagc
     3961 aattgtggat tttccgcttg ccgaaagacc ggtaaaccag ataaccatcc ctttgttgcc
     4021 tgtcttttcc tgtctgtatt tgctgtctaa gttgcgatgc catacgacat ta
//
LOCUS       DAOPWC010000093         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_22797_length_3991_cov_3.320630, whole genome shotgun sequence.
ACCESSION   DAOPWC010000093 DAOPWC010000000
VERSION     DAOPWC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22797_length_3991_cov_3.320630"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1654)
                     /locus_tag="PKJ65_02930"
     CDS             complement(53..1654)
                     /locus_tag="PKJ65_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ40825.1"
                     /translation="MKIEDIRRMAHDIIDQCAVENDYIEYKKSAADIVKDGILKTVCA
                     YANNYMNREIGLLLIGIEEEDDEATGRKAVPVRPISGIDEAKIETTENELKYLLSYIH
                     PKPVYHLVQDKIDNRFYIILAVEPGNNGPYETDQKAEKDSKIKLKAGRYIRVRRDTRL
                     PNKREEFELLKKFADFHFSSELNETATLDDLDYEYMKEYMVRTNSAEDIRTLSKLEMA
                     KAMHLIDSSEYGGYRARNFAVLMFADAPEKFIPYAYVEVIREISGTDKMESKSFKGPV
                     WIQAQRVRDYFRDTIMASYTVREPGKPGAHRVFNWPLEMFEELATNCILHKEYSRKQY
                     IGIYVYRDHLSFINYNRPVPPITIADLNNKVVFDNRHYLNDELKEMFFRLNLIQSYGS
                     GIRRAKKAMADNGSPKLVYSPDNDTDDYTQVVAYINEEFLRIQEEEEVRQSSKQQSGR
                     EDFHSTDERSLKEVLKEVLKEADYRKIISIADVIDTKGSITPAEAKEACGKSESTTWR
                     YLSILTSTGLVVSEGSTNKTVYRRI"
     gene            complement(1644..1862)
                     /locus_tag="PKJ65_02935"
     CDS             complement(1644..1862)
                     /locus_tag="PKJ65_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ40826.1"
                     /translation="MNEHDFMTICQAADKWNITVRRAQVLCAEGRIEGAFRLGRAWII
                     PRDAEKPADARIKSGKYVKVKDGGANED"
     gene            2109..2714
                     /locus_tag="PKJ65_02940"
     CDS             2109..2714
                     /locus_tag="PKJ65_02940"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNZ40827.1"
                     /translation="MSNGYADKIRERVLSATNGTVFTTSDFSDIADNNTIRQNMLRLV
                     NEGVLKRILGGVYEKSKYSKLLGEYVAVNPDAVAQALARSYHWTIAPCGNTALNMIGL
                     STQVPATWSYISDGPYRKYEWNSTCIEFKHRTNKEITGLSYMTALLIQAIKTLGKENI
                     TQDIIETLSEKLTRKEKDAALKEASRSADWVYRIIKRICRN"
     gene            2791..3291
                     /locus_tag="PKJ65_02945"
     CDS             2791..3291
                     /locus_tag="PKJ65_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40828.1"
                     /translation="MQYHIRRAKEKDAEDILRLLGQIAQLHHEKRPDIFKSNASKYNN
                     KLILEKIKRPEEYITVAVDETDKVAGYLFCVFDIVKNHPVQQDRKILYLDDLCVDEDK
                     RGSGIGSLLINHAYEYAKKEHCDAVELNAWEINERAVKFYADHGFTVKYRRMELIVDS
                     KNDLER"
     gene            3294..>3991
                     /locus_tag="PKJ65_02950"
     CDS             3294..>3991
                     /locus_tag="PKJ65_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNZ40829.1"
                     /translation="MKVYVVQPSYPKNGDEKKSTVDFMLQSLDSVGTDADLILLPEFS
                     NIPGASQYDEMIADYNRNTDILTKRVIATAKRTNSLVAVNMAHKTDTGFRNSTLLYNG
                     NGELIYRYDKQHLTYAEKELLKLDYSYRYRTDIPCFVDVGGLRLAFMTCYDTYFLEYI
                     EYIRSKKPDIILICSYQRSEEEDIILSQARIISSRCNAYVLRASYSMGNTAKGGHSLV
                     CDCDGTVLADMEQLT"
BASE COUNT         1214 a          849 c          804 g         1124 t
ORIGIN      
        1 atttcatatt atacatctaa ataaagattt ataccccaac atcatctggg gattatattc
       61 tcctgtaaac agtcttatta gtgctacctt cggaaaccac cagccctgta ctggtcaata
      121 tgctaagata gcgccaagtt gttgattcag acttgccaca ggcttcctta gcctctgcag
      181 gggtaatact gccttttgta tctatcacat ccgcaataga gatgatctta cgatagtcag
      241 cttccttcag aacttctttc aaaacttctt tcaaacttct ttcatctgtt gaatgaaaat
      301 cctcgcgtcc ggactgttgc tttgaagatt gcctaacttc ctcctcttcc tgtatcctca
      361 gaaactcctc gttgatatag gccacgacct gtgtatagtc atcggtatca ttatccggac
      421 tgtagacaag ttttggagaa ccgttgtcag ccattgcctt ctttgcacga cgaatcccgg
      481 aaccatagga ttggatcaga tttaaacgga aaaacatctc tttaagctca tcattcagat
      541 aatgccgatt atcaaagacc actttattat tcagatcagc tatggttatc ggcggaacag
      601 gacgattata attgataaaa gacaggtgat ccctataaac ataaatcccg atgtactgct
      661 tccgtgagta ttccttatgt agaatgcagt tggtcgccag ctcttcaaac atttccaatg
      721 gccagttaaa gaccctgtgc gctcccggct tgcccggctc tcgaacagtg taagaggcca
      781 tgatggtatc tcggaaataa tctcggacac gctgtgcctg aatccataca ggtcccttaa
      841 agcttttgga ttccatctta tctgttccgg aaatctctct gattacctct acgtaagcgt
      901 atggaatgaa cttttccggt gcgtcagcaa acatcagcac agcgaagttc cgtgcgcggt
      961 agccaccata ctcgctgctg tcgatcaaat gcatggcctt cgccatctct agcttggata
     1021 atgtgcgaat atcctctgcg gaattagtgc gtaccatata ctctttcatg tactcataat
     1081 ccagatcatc cagcgttgca gtttcgttca attctgaaga aaaatgaaaa tctgcgaact
     1141 tcttgagaag ttcaaactcc tctcgtttgt tgggcagacg cgtatctcgg cggacccgga
     1201 tatatctacc tgctttcagc ttgatttttg aatccttttc cgccttttga tcggtctcat
     1261 aaggaccgtt atttccaggc tcgacagcaa gaatgatata aaaccgatta tctattttat
     1321 cctgaaccag atgatacacc ggtttgggat ggatataaga caaaaggtat ttcaattcat
     1381 tttccgttgt ctcaatcttt gcctcatcga ttccggatat cggacggaca ggaactgctt
     1441 tgcgaccagt agcctcatca tcctcttctt cgatccctat aagcagaagc ccgatttccc
     1501 ggttcatata gttattagca taagcacaga cagtttttag gataccatcc ttcacgatat
     1561 cggctgcgga ttttttgtat tcgatataat cgttttctac ggcacactga tctataatat
     1621 catgagccat ccggcggata tcttcaatct tcattagcgc caccatcctt taccttaaca
     1681 tatttgccgc tcttgattcg cgcgtctgca ggcttttcgg catctctcgg aatgatccag
     1741 gcacgaccca gtcgaaaggc gccttcaata cgaccttccg cacacaacac ctgtgcgcga
     1801 cggacggtaa tattccattt gtctgcagcc tggcatatcg tcataaaatc atgttcattc
     1861 atcaatacaa actcctccgt caaaacaggc aaccccattt tatacattta tgcgaacaat
     1921 ttcaagccct tatatttgaa gaattgtgaa atatgttaca agtcagtttg acataattag
     1981 catatctttg taacaaagta tgcttataaa ttactcatct ataaatttaa caactgatgg
     2041 agaaagtgca aactattgca atattgtaac gaatagtata taattttcgt tacaaggagg
     2101 gtactcttat gagtaacgga tatgcagaca aaataaggga gcgcgtgctt tcagcaacaa
     2161 atggaacagt ctttactaca tcagactttt cagatattgc agataataat actattcggc
     2221 aaaatatgct tcgcctcgta aacgaaggtg ttttgaagcg cattttaggc ggtgtatatg
     2281 aaaagtctaa atacagcaaa ttgcttggag agtatgtagc tgttaatccc gatgcggtgg
     2341 cacaggcact tgctcgcagt taccattgga caattgctcc ttgcggtaat acagccttga
     2401 atatgatagg tctctcaacg caggttccgg ccacctggtc atatatcagc gacggaccat
     2461 atagaaagta tgagtggaac tcgacatgca tagaatttaa gcacagaaca aacaaagaga
     2521 ttaccggtct atcctatatg actgctcttt tgattcaagc tattaaaaca cttggtaaag
     2581 aaaacattac gcaagacatt attgagacac tttcggagaa attgacacgg aaggaaaaag
     2641 atgcagccct aaaagaagca tcaagaagtg ccgattgggt ttacaggata attaagcgga
     2701 tatgtaggaa ctaagcagaa taatgcgcct aaacatagat ttataggata taataaagaa
     2761 tatagaagat atggaaatga gacattgtaa atgcaatatc atataagaag agcaaaagaa
     2821 aaagatgcgg aagatatcct gaggttgctt gggcagatag cgcaactgca tcatgaaaag
     2881 cgaccggaca tatttaagag caatgccagt aaatacaata ataaactgat acttgaaaag
     2941 attaagagac cggaagaata tataaccgtt gcggtggatg aaaccgacaa agttgctgga
     3001 tatttattct gtgtgtttga catagttaaa aatcatcccg ttcagcagga cagaaaaata
     3061 ttgtatttgg atgatttatg tgtggatgaa gataaaagag gctcaggtat cggctccctc
     3121 ctcataaatc acgcatatga atacgcaaaa aaggaacact gtgatgccgt agaacttaat
     3181 gcctgggaga ttaacgaaag agccgtaaaa ttctatgcgg atcacggatt taccgttaaa
     3241 tacagaagaa tggaattaat tgttgacagt aaaaatgatt tagagaggta gctatgaaag
     3301 tatatgttgt acagccgtct tatcctaaga atggtgatga aaagaaaagt accgtggact
     3361 ttatgctgca atcgctggat tccgtaggaa cggatgccga ccttatactg ctccccgaat
     3421 tttccaatat tcccggagca tcacagtatg atgaaatgat tgccgattac aaccggaata
     3481 ccgatattct gactaagaga gttatcgcca cggcaaaaag aacaaacagc cttgtagccg
     3541 tcaatatggc acataagacc gataccggtt tccgaaacag cacgctttta tataacggaa
     3601 acggagagct tatttacaga tatgacaaac agcatcttac atatgcggaa aaagagttgt
     3661 taaaactgga ttactcttac agatacagaa ccgatatccc ctgctttgtt gatgtaggcg
     3721 gactgagact ggcattcatg acctgttacg atacatactt tttagagtat attgagtata
     3781 tccggtcaaa gaaacccgat atcattctta tctgttccta tcagcggagt gaagaagaag
     3841 acattattct ttcacaggca aggataatat cctccagatg caatgcttat gtcttaagag
     3901 catcctactc catgggcaat accgccaaag gcggtcactc ccttgtctgc gactgtgacg
     3961 gaacggttct ggcggatatg gaacagttga c
//
LOCUS       DAOPWC010000094         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_23172_length_3947_cov_3.209661, whole genome shotgun sequence.
ACCESSION   DAOPWC010000094 DAOPWC010000000
VERSION     DAOPWC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23172_length_3947_cov_3.209661"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKJ65_02955"
     CDS             complement(<1..658)
                     /locus_tag="PKJ65_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ40830.1"
                     /translation="MGIHNRMTSPFGISHPFTLLMYYTALFSCIFIFRNPVFILFLFI
                     ACCLMAIYYAGADSFFKSMKWIALIGVAVLVLNALLVHRGSTVLFFLFKNPVTKEALI
                     YGVYNMLMLMSVMATFISFNVLLDSPKFLYLFSGILPKLSFVFDMALRYIPLFRKRAS
                     DLAAVRSVNSDREHMTFIQKIKTYGIYLKALTAWNLEEGMETALSLKVKNYGTHNKVH
                     Y"
     gene            complement(637..1041)
                     /locus_tag="PKJ65_02960"
     CDS             complement(637..1041)
                     /locus_tag="PKJ65_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNZ40831.1"
                     /translation="MKRAVYIIFLLCLIFVLITGCTKDTDVSEGYVYVTIDATKADKG
                     IILSEYAVSLKEGTTVFDITFAACKDNGILMSSRGTVVTRYIEGIADLYEFDLGPASG
                     WVYYVNDISATIGCGLYEIKEGDKIRWEYIIE"
     gene            complement(1041..2447)
                     /locus_tag="PKJ65_02965"
     CDS             complement(1041..2447)
                     /locus_tag="PKJ65_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40832.1"
                     /translation="MRKALTVILAVCILLSTVSVSASEDVYVKISQLYLEKTALSVWE
                     ALGLSLNGYRIPDSYYATLKEDIVSRNGVYRTSLEYAKIIILLKQGGKNPKDFHGYNL
                     VSLLQSFKNIDKSGINGVIFTIFALNDIQSESNAIWTKDNLLPLLLEYQNEDGGFPLV
                     KGWGSDNDLTAMAVSALAYYPDNKQAKEAMDKALSYLSGKLDNDGFMYYQNSDSSEIL
                     SQIIIELSTAGISLNDSRFVRNNKTLYDTLIEKYMTKDFRFRHSISQEANDMATEQAY
                     LALSAIKTGYVYAKKEKTIVPDPTPSPSPTASSTVPPEIIHEPTNPDVAKKTGFSDEE
                     RISKRYYSDVLTAYTEKLVVGDGSNLRPLDNLTRAEACAILYNVAQLGINVFIPRFKD
                     IGKESWYSKYVIACYLNGIMFGADEVCFEPDSYITVFELTESINRMSGTDVVYDDGFI
                     TREAAISLIVRIFYGGFD"
     gene            complement(2448..>3947)
                     /locus_tag="PKJ65_02970"
     CDS             complement(2448..>3947)
                     /locus_tag="PKJ65_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40833.1"
                     /translation="AGWMYVLNDESSWNSVKDQKLEKDDYVVLYYIKDWMNCSYSTFG
                     VADEYEVDAFTTVKLNLKHQVTDPVTWAASNENLAGAVITVDGKKTDAVTDEFGNAYL
                     TFKDKGTHVISAEKYVDGINTISRSYAYIDVQGKGVAVNIYTTDRTYRACVEPKGFDL
                     AKYDASSECEFSALHAIIAALNSNGFDAADPKIANYNKGSFISALAGVEASGNSAWMY
                     TVNNQTSWFAVNEQPLTDGDVLDIYLLNDWATDTYSYFDMTSIKVQGGENIRVRLYRH
                     VLDFVTWETSVHPYANCEITVNGRKAGYVTDNDGYVNLKFNKKDIYEISVLPKEDNIS
                     RASVTVEVTNDSDISLEQYNDYKEVTDKYLTYMEKAVGKYIMNGDQDNNLRPADEITK
                     AEFLTMLVRASKLDVPGKYRKRYTDVVAGNWFGSFAQAVFRYGLAKHDKGFLYPDAAV
                     SSDIALYALNTAFASDAALSDAQKAVFAEGLTREEAAYLIISYMEATGR"
BASE COUNT         1124 a          914 c          705 g         1204 t
ORIGIN      
        1 cataatgcac tttattatgc gtgccgtagt ttttgacttt tagactcaac gccgtttcca
       61 taccctcttc caaattccag gcggtcagag cttttaagta tataccgtat gttttaatct
      121 tttgtataaa agtcatatgc tccctgtcgg agtttaccga ccggaccgcg gcaaggtcgg
      181 atgctctctt tctgaataaa ggaatatacc gcaaagccat atcaaaaaca aaagacagtt
      241 taggcagtat accggaaaaa aggtacaaaa acttagggct gtccagtaat acattaaagg
      301 atataaatgt ggccattacc gacatgagca taagcatgtt gtacactccg tatatcaatg
      361 cttcttttgt taccggattt ttgaaaagaa agaataagac tgtactgccc ctgtggacaa
      421 gcaacgcgtt aagaacaaga accgcaacgc ctatcagagc tatccatttc attgacttaa
      481 agaagctatc cgctccggca tagtaaatcg ccataagaca acacgctata aaaagaaaca
      541 ggatgaagac gggatttctg aatataaata tgcaggaaaa taaagccgta tagtacatca
      601 acagtgtaaa cggatgagat ataccgaacg gacttgtcat tctattatgt attcccacct
      661 tattttatcc ccttctttta tttcatataa accgcagcct atagttgccg atatgtcatt
      721 cacataatac acccaaccgc tggcaggtcc taaatcaaat tcatataaat cggcaatccc
      781 ttcaatataa cgggtaacta ccgttcctct ggaggacatc agaatcccgt tatctttaca
      841 tgccgcaaag gtaatatcaa ataccgtcgt gccttctttc agacttaccg catattccga
      901 caagattatt cctttatccg ctttggttgc atctatggtc acgtatacat acccttccga
      961 aacatcggta tcctttgtgc aaccggttat taagacaaat attaagcata ataaaaaaat
     1021 aatatatacc gcacgtttca ttaatcaaaa cctccgtaaa aaatcctcac tatcaatgat
     1081 atagctgctt ctcttgttat aaaaccgtca tcatacacta catccgttcc ggacatcctg
     1141 ttaatacttt cggtcagttc aaaaaccgtt atatatgagt cgggctcaaa acatacttca
     1201 tccgccccga acattattcc gttaagatag cacgcaataa catattttga ataccaactc
     1261 tccttcccga tatccttaaa tctgggaatg aaaacattga tacccaactg tgcaacattg
     1321 tacagaatgg cacatgcctc agcccgggta agattatcta aaggtcgcaa attgcttccg
     1381 tcacctacaa ccagtttttc agtatatgct gtcaaaacat cgctataata ccgctttgaa
     1441 attctttcct catcggaaaa tcccgtcttc tttgcaacat ccggatttgt cggttcatgg
     1501 ataatttccg gcggtactgt tgaagatgca gtcggtgaag gtgatggggt cggatccggt
     1561 acaatagtct tttctttctt tgcatataca taaccggttt taatcgcact taaggctaaa
     1621 taagcctgtt cggttgccat atcgttggcc tcctgtgaaa tactatgcct gaaacgaaaa
     1681 tcttttgtca tatatttttc aatcaaagta tcatacagtg tcttgttgtt acgaacaaat
     1741 ctgctatcgt tcaaggaaat gccggcggta cttaattcaa taataatctg agaaagaatt
     1801 tccgaactgt cggagttctg ataatacata aatccgtcat tgtccagttt accgctcaaa
     1861 taggataaag ccttatccat ggcttctttt gcctgcttat tgtctggata atatgccagt
     1921 gcggacacag ccattgcagt taaatcatta tccgaacccc acccttttac caacggaaaa
     1981 cctccatctt cattttgata ttccaaaagc agaggaagca gattatcttt tgtccatatc
     2041 gcattacttt ccgattgtat gtcgttcaat gcgaaaatgg tgaaaataac gccgtttatt
     2101 cccgatttat ctatgttctt gaatgactga agcagtgaaa caaggttata tccgtgaaaa
     2161 tccttgggat tctttccgcc ctgctttaaa aggattatta tcttggcata ctccaaagat
     2221 gtcctgtaga ctccgtttct gctcacaata tcttctttta atgtcgcata atatgagtcc
     2281 ggtattctat aaccgttcag tgaaagtccg agagcttccc atacggaaag agccgttttt
     2341 tcaaggtata actgtgatat ttttacatac acatcttctg aagcacttac actgacagtg
     2401 ctcagcaaaa tgcatacagc caatatcaca gtcagagcct ttctcattta tctgcctgta
     2461 gcctccatat agcttattat caggtaggca gcttcttctc gcgttaaacc ttcagcaaat
     2521 accgctttct gtgcatcact caaagcggca tccgatgcaa atgccgtatt caaagcatat
     2581 agcgcaatat ccgatgaaac agcggcatcg ggataaagga atcctttatc atgctttgcc
     2641 agaccgtatc tgaataccgc ttgagcaaaa cttccgaacc agttgcctgc aacgacatcg
     2701 gtatatctct tcctgtattt acccggtacg tccagtttgg atgcacgtac gagcattgta
     2761 aggaattccg ctttggtaat ttcatcggca ggtctcagat tattatcctg atccccgttc
     2821 atgatatatt tacctaccgc cttttccatg tatgttaagt acttatcggt tacttccttg
     2881 taatcgttgt attgctccaa agatatatcg ctgtcatttg ttacttcaac ggtcaccgat
     2941 gctctggaaa tattgtcttc cttgggcaat acggatattt cataaatgtc ttttttgtta
     3001 aacttaagat ttacatatcc gtcgttatcg gttacatatc ccgctttacg tccgtttacc
     3061 gttatctcac agtttgcata aggatgaacc gatgtttccc atgtgacaaa atccagtaca
     3121 tggcgataca atctcactcg tatattctca ccgccttgga cttttatgct tgtcatatca
     3181 aagtaagaat aggtatcggt tgcccaatcg ttcaaaagat aaatatccag aacatctccg
     3241 tcagtcagcg gctgttcatt taccgcaaac caggatgttt ggttatttac cgtatacatc
     3301 catgcggaat tgcccgaggc ttccactccc gccaatgcgg atataaaaga acccttgttg
     3361 taattagcaa ttttaggatc ggctgcatca aaaccgttac tgttgagtgc cgcaattatg
     3421 gcatgtaaag cggaaaactc acactccgat gatgcatcgt attttgcaag atcaaaccct
     3481 ttaggctcca cacacgctct gtaggtccta tcggttgtat atatgtttac agccacacct
     3541 ttaccctgca catctatgta tgcataggag cgggagatgg tgtttatacc gtcaacatat
     3601 ttctcggcgg atattacatg tgtaccttta tctttgaatg taaggtaggc attgccgaat
     3661 tcatccgtta ccgcatcggt cttctttccg tccaccgtta taaccgctcc tgcaagattc
     3721 tcattagaag cagcccatgt aacggggtcg gttacctgat gcttaaggtt gagctttact
     3781 gtggtaaatg cgtcaacttc atactcgtcc gctacaccga atgtggaata ggagcagttc
     3841 atccagtctt tgatatagta gagaactaca taatcatctt tttccagctt ctggtccttt
     3901 acggaattcc atgacgattc atcgttcaga acatacatcc aaccggc
//
LOCUS       DAOPWC010000095         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_23224_length_3941_cov_3.467319, whole genome shotgun sequence.
ACCESSION   DAOPWC010000095 DAOPWC010000000
VERSION     DAOPWC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23224_length_3941_cov_3.467319"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKJ65_02975"
     CDS             <1..232
                     /locus_tag="PKJ65_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease IV"
                     /protein_id="HNZ40834.1"
                     /translation="TRIMHVHFSQIEYTQGGEKKHLTFEDDKYGPYFQDLIPVLYKYD
                     MKPVIICESDNTMATDALKSKIMYEQYMKKDL"
     gene            241..1317
                     /locus_tag="PKJ65_02980"
     CDS             241..1317
                     /locus_tag="PKJ65_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNZ40835.1"
                     /translation="MFSKRVDCMKKIMEEKELDGAVITSRDNTLYLSSFSGSSSILIF
                     TRDKAYFATDFRYIEAAELISAFGYEVVLIQEGIKSIVSLLSKLFTKSALRIGFEKTV
                     SYGYFEALKKGLKDCEYIDVSDRLLAIRSVKDQKELSLMAKAASIADIAFTHILNTVK
                     AGMTEIEVAAELEYVMKKNGASFPAFETIAISGKKTSMPHGVPDHKKLMNHDPVTLDF
                     GANYKGYCSDMTRTFFLGSPSPKMKKIYNIVYDAQKSAQDYVREGVTGADVDKIARDI
                     IDNEGYGKYFGHSTGHGVGLLVHEEPRLSTQSTTVLKSNMTVTVEPGIYIPDAGGVRI
                     ENTVVVQEDGCYTLNHSNKELIII"
     gene            1319..1783
                     /locus_tag="PKJ65_02985"
     CDS             1319..1783
                     /locus_tag="PKJ65_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40836.1"
                     /translation="MNYTIRKLDAESFICIAELRGYAKDDEDFIERYNRVVDKKDDCY
                     AVFVNDECIADVTLTYVSSDKAAAEENIRAYMSGLLVKENYRNKKIATALIMHLTEEL
                     KEKGYKELSVLSDTDNVVACKLYNRLGFRKENTYNVEDMSFDLLVYGIYRAL"
     gene            1926..2315
                     /locus_tag="PKJ65_02990"
     CDS             1926..2315
                     /locus_tag="PKJ65_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNZ40837.1"
                     /translation="MADDVLKINGLETSLGQLKVTNEVIAILAGIAAMEEDGVYAMSG
                     GITGELAQALGIKNLSKGVRIETDEEGIHVNIYIIVELGVRIPEVAWSIQEKVKKLVE
                     KMTGSKVTEVNIHVQGVNIHKPGEEQK"
     gene            2312..2845
                     /gene="amaP"
                     /locus_tag="PKJ65_02995"
     CDS             2312..2845
                     /gene="amaP"
                     /locus_tag="PKJ65_02995"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HNZ40838.1"
                     /translation="MKFFKKGRKFFYAVNIILIVVLCAVTIHACLDPMDYERAFEDFV
                     DARFFSVQRGVIFLIALLILVFEAFFIVSLLRKEEKPVETVNLTTPTGQISITAYSLE
                     TMVKSIVSRFDSVADVRTKVDFSNSQVGMDISVKVKSSVLIPEISSKIQSIVKEEVLK
                     STGIELHSIRVFVDGIN"
     gene            2857..3261
                     /gene="nusB"
                     /locus_tag="PKJ65_03000"
     CDS             2857..3261
                     /gene="nusB"
                     /locus_tag="PKJ65_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNZ40839.1"
                     /translation="MGRRKTREHALRLVYQIQINDDDINEQMEQYFLDNDVKNTAKEY
                     IKKAVTYINENCKDIDSVLSMYLAKNWRIERIPKIELAIMRLAYYEILNLEDVPKSVA
                     INEAVELAKTYGSDDAGKYINAVLANIQNGEQ"
     gene            3261..>3941
                     /gene="xseA"
                     /locus_tag="PKJ65_03005"
     CDS             3261..>3941
                     /gene="xseA"
                     /locus_tag="PKJ65_03005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ40840.1"
                     /translation="MEKALTVSQLNEYIKQLVSSDEVLESLHVEGEISNCTIHYSGHI
                     YFSIKDDKCAIRCVMFAGYTKNLKFEPADGVKVTVYGYLSFYEKTGQCQIVVTNMLES
                     GTGDLFIKFENLKKKLEKEGLFDTAKKKPLPFLPETIGVITSSTGAVLQDIRNILNGR
                     FPNYGFILYPATVQGVDAHKTVIEGLEYFNKNKNADVIIIARGGGSVEDLWPFNEEVV
                     ARAIFNSAL"
BASE COUNT         1268 a          640 c          945 g         1088 t
ORIGIN      
        1 gacaagaata atgcatgtgc atttttccca gattgaatat acgcaaggcg gcgagaaaaa
       61 acaccttaca tttgaagatg acaagtacgg tccctatttc caggacctta taccggtact
      121 gtataagtat gatatgaaac ctgttataat atgcgaatcg gataatacca tggctaccga
      181 tgcattgaag agtaagatta tgtatgagca atatatgaag aaggatttat gaggtgccgt
      241 atgttcagta aaagagttga ttgtatgaaa aagataatgg aggaaaagga actcgacgga
      301 gccgttataa cgtccagaga taatacgctt tatctttctt ctttttccgg gtccagcagt
      361 atattgatct ttacacgtga taaagcatac tttgcaacgg attttcgtta cattgaagcg
      421 gccgaactga tatccgcttt cggctacgaa gtggtgttga ttcaggaagg cataaaaagc
      481 atagtctctt tgttatccaa gctttttact aaaagtgcat tacgtatagg atttgaaaaa
      541 accgtttcat acggctattt cgaggcttta aaaaaaggtc tgaaagattg cgaatatatt
      601 gatgtttccg accggttgct cgccatacgc tcggttaaag accaaaagga attgtctctg
      661 atggcaaaag ccgcttccat agcggacatc gcttttactc atatactgaa tacggtaaaa
      721 gcgggaatga cagaaataga ggtagctgcc gaactggagt atgtgatgaa gaaaaacgga
      781 gcatcctttc cggcttttga aaccatagcg atatcgggta aaaagaccag tatgcctcac
      841 ggtgttcccg accataagaa gcttatgaat catgatccgg ttactttgga tttcggagca
      901 aactacaaag gatattgttc cgatatgacc aggactttct ttctgggttc tccgtcacct
      961 aagatgaaga agatatataa catagtgtat gacgcacaaa agagtgcgca ggattacgtc
     1021 agagaaggtg taacgggagc ggatgttgat aagattgcaa gagatataat agataatgaa
     1081 gggtacggaa agtatttcgg gcacagtacg ggacacggag tgggtttgct ggtacatgaa
     1141 gaacccaggc tttccaccca aagcacaacc gttctgaaaa gtaatatgac cgttaccgta
     1201 gagccgggaa tatatattcc cgatgcgggc ggagtccgga tagaaaatac cgttgtcgtg
     1261 caagaagacg gctgctatac tcttaatcac tcgaataaag agttgattat tatttaaaat
     1321 gaattatacg ataagaaaac ttgatgccga atcatttatc tgcattgccg aacttcgagg
     1381 atatgcaaaa gatgatgagg attttattga gcgctataat cgggttgttg ataaaaaaga
     1441 cgattgctat gcggtttttg taaatgatga atgtattgcc gatgtcacat tgacatatgt
     1501 ttcaagtgac aaggctgcgg ctgaagaaaa tataagggca tatatgtcgg gattattggt
     1561 caaagaaaac taccgtaaca agaagattgc gaccgctttg ataatgcatt taactgagga
     1621 gttgaaagaa aaagggtata aggagttgtc ggttttatcg gataccgata atgttgttgc
     1681 ctgtaaactg tataacagac tcgggttccg aaaagaaaac acctataatg tagaggatat
     1741 gtcctttgat ttattggtgt acggaatata tcgggcactt taagggatgt tctatgtttt
     1801 ttaactttta aattaatttt tggttcactc taaaattttg tgggatttag gattcttgac
     1861 gtaagtctct ttcggttgtg ttgtaaatat atcgagcaaa atgtataatt actgtgaggt
     1921 atgttatggc tgatgatgtt ctgaaaatta acggactgga aacatcctta gggcaactta
     1981 aggtgactaa cgaggtaata gcgatattgg ccggaattgc cgcaatggaa gaagacggcg
     2041 tgtatgcaat gagcggaggg attaccggcg aacttgcaca ggcattgggg attaagaatc
     2101 tttccaaagg cgtcaggatt gagactgatg aggaaggcat tcatgtcaat atatatatta
     2161 ttgtggaatt gggagtaaga atacccgaag tggcatggag tatacaggaa aaggtaaaaa
     2221 aactggtgga aaaaatgacc ggcagtaaag ttacggaagt taatatccat gttcagggtg
     2281 taaatataca taaacccggg gaggagcaga aatgaaattt tttaaaaagg gcaggaagtt
     2341 tttttatgcc gtaaatataa tattgattgt tgtcttatgt gccgttacga tacatgcatg
     2401 cctcgatccg atggattacg agagagcttt cgaggatttt gtcgatgcaa ggtttttctc
     2461 ggtacaaaga ggggttatat tcctgattgc attattgatt ctggtatttg aagctttctt
     2521 tatagtgtct cttttacgca aagaagaaaa acctgtagaa acggtcaatc tgaccacccc
     2581 gacaggacag atcagtatta cggcctattc gttggaaact atggtcaaga gtatagtaag
     2641 tcggtttgat tcggtggccg atgtgagaac caaggtggat ttcagcaatt cgcaggtagg
     2701 tatggatata tcggtaaaag tcaagtcatc ggttctcata ccggagatat cttccaagat
     2761 tcagagcatt gttaaagagg aagtgttgaa aagtaccggc atagaattgc atagcatcag
     2821 ggtttttgtg gacggaataa attaaaaggg actgttatgg gaagaagaaa aacaagggaa
     2881 catgctcttc ggcttgtata ccagatacag ataaatgacg atgatataaa tgagcagatg
     2941 gaacagtatt tccttgataa tgatgtaaag aataccgcaa aggaatatat aaaaaaggcg
     3001 gtaacatata tcaatgagaa ctgcaaagac atcgactcgg tgctttccat gtaccttgct
     3061 aagaactggc gtattgaaag aattccaaag atagaactgg caatcatgag acttgcatat
     3121 tatgagatat tgaatcttga ggatgtaccc aaatccgtgg caataaatga agccgtcgaa
     3181 cttgccaaaa cctacggcag tgatgatgcc ggaaaatata tcaatgcggt gctggcaaat
     3241 attcaaaacg gagagcagtg atggaaaaag cccttaccgt atctcagctc aatgagtata
     3301 tcaaacaact tgtttcttcc gatgaagtat tggaaagtct gcatgtggaa ggtgaaatat
     3361 ccaactgcac cattcactat tccggtcata tatacttttc cataaaggat gataaatgcg
     3421 ctatacgatg tgtaatgttt gccggatata ccaaaaacct caagtttgaa ccggcggacg
     3481 gtgtgaaggt tacagtttac ggataccttt ctttttatga aaaaaccggg cagtgtcaga
     3541 ttgttgttac caatatgtta gaaagcggga caggtgattt attcatcaaa ttcgagaatc
     3601 tcaagaagaa actggagaaa gaaggtcttt ttgatactgc aaaaaagaaa ccgttgccgt
     3661 ttttgccgga aaccataggt gtaattacct caagtaccgg agcggttttg caggatataa
     3721 ggaacatact gaacggaagg tttcctaatt acggattcat tttgtatccc gcgaccgttc
     3781 agggagttga tgcgcataaa accgtaattg aaggactgga atattttaat aagaataaaa
     3841 acgccgatgt cataataata gcaagaggcg gcggttcggt tgaagacctt tggccgttca
     3901 acgaagaagt cgtggcacgg gccattttta actcagcgct c
//
LOCUS       DAOPWC010000096         3923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_23376_length_3923_cov_3.111686, whole genome shotgun sequence.
ACCESSION   DAOPWC010000096 DAOPWC010000000
VERSION     DAOPWC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23376_length_3923_cov_3.111686"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKJ65_03010"
     CDS             <1..843
                     /locus_tag="PKJ65_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HNZ40841.1"
                     /translation="TLNAPLGFIEDMKKYSSHILNVQGGSVTKEMVKHRKSYDIVNTA
                     HPFTGIHLTEKGLDYLEQYIKDVRSVIGYEVPLAVDHFGHVCVEDCIKFARRMEPYNI
                     AWIEDMIPWIYTDQYVRLKNSTTIPICTGEDIYLKEGFVELIKAGAVSIIHPDILTCG
                     GALELKKIADIADEFGVAVAVHMAESPIACMAAAHVCAAMRNVLAMEFHSVDVPWWSD
                     IVVGLPKPIIQDGYCTVPDKPGLGIDALNEEVIKEHINPQIPGMWEETSKWDKEFSND
                     RIWS"
     gene            887..1789
                     /locus_tag="PKJ65_03015"
     CDS             887..1789
                     /locus_tag="PKJ65_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HNZ40842.1"
                     /translation="MHFNSKEDIIEMTPLWKGERLEDGRPYVDPKYLTELRKMTLEEV
                     WKPIYVKGYENQFEGRLKTLHDDGRKLIGRAVTCTFCPTRPDLHEIMFRIGSNEGRKG
                     NYNQWVIDSLVEGDVVVADMYDKVYKGTFLGGNLTTAIKNRTKTGGGVIWGGIRDVEQ
                     MKQAEGIQVYYRGIDPTPIRECVMTGFNTPTRIGNAICLPGDIVFGAGGGVLFIPSHL
                     VAEVVEGAAKTHIKDEFGFEMIKQNKFTTAQIDRNTWTVEMLDMLVDFIQNDERGKNY
                     RDIDWSKEYYMAKHGDPNDTQTAL"
     gene            1799..2491
                     /locus_tag="PKJ65_03020"
     CDS             1799..2491
                     /locus_tag="PKJ65_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ40843.1"
                     /translation="MKLRWIGQSGYILEDDTTEIIIDPYLSDIVNKVANRPRLVPSPI
                     QPEDINADYVICTHDHLDHIDPETIERMNKDTVCFIAPSSCKERLSALGCKNIKTIDA
                     GEQISAGKFQITAVFANHSVDAIGIIAEQDSIKLYFSGDTLYHEKLEELKESGITVMF
                     VCINGKLGNMNADEAAKLTKIINPLVGVPTHYGMFASNTEDPEKYTSKIDNGFIMEFN
                     KEYTISELLRGE"
     gene            2494..3237
                     /locus_tag="PKJ65_03025"
     CDS             2494..3237
                     /locus_tag="PKJ65_03025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ40844.1"
                     /translation="MQHKLSGKRALITGSGRGIGRAIAIELAKNGADIIVHYLGNVKS
                     AEQVKKQIEASGGSCSIIQADLCKPETLEGLVEKIGGVDILVLNASVQYRRQWENISL
                     EEFYDQINCNLCASMLLIQQAVPYMRKKHWGRIITIGSVQEAKPHPDMLVYSASKAAQ
                     TNMVKSLAVQLAKDGITVNNVAPGAINTDRNKDVLKDKEYYKKVTDSIPAGFIGEPAD
                     CAGIVSLLCSEEGRYITGQSIYIDGGKCL"
     gene            3255..>3923
                     /locus_tag="PKJ65_03030"
     CDS             3255..>3923
                     /locus_tag="PKJ65_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNZ40845.1"
                     /translation="MSHTLLRFFEEQKVQSNKRIEGDIQKYRKAFAKIKVVNSQGDLI
                     ENVKGRIKQKTHDFKFGCNIFMLDQFPDEEHNKAYRKEFKKLFNYTVAPFYWQDFELK
                     DGEPRLGKNPVNVYRRPSIEKILKYCGENNIDVKGHPLFWHLFLPQWLVQDKNEVYYR
                     IERRLFEIAEVFKDRIRDWDVVNESLSRPPHRENYQLPYDYVNRIFDLADRYFPKNRL
                     FINDT"
BASE COUNT         1313 a          660 c          932 g         1018 t
ORIGIN      
        1 actttgaatg ccccgctcgg ctttattgag gatatgaaaa agtattcttc acatatcctg
       61 aatgttcagg gcggttcggt aacaaaagag atggtgaagc ataggaagag ttatgacatt
      121 gtaaataccg ctcacccgtt tacaggaatt catcttaccg aaaaaggact ggattatctg
      181 gaacagtaca taaaagatgt ccggtcggtt atcggttatg aagtaccgct tgcagtggat
      241 catttcggcc atgtttgcgt tgaagactgc ataaaatttg cccgcagaat ggagccgtat
      301 aatattgcat ggattgaaga tatgattcct tggatatata ccgatcagta tgtaagactg
      361 aaaaattcca ccaccatacc gatatgtaca ggagaagata tttatctgaa agaaggcttt
      421 gttgaattga taaaagcagg agcggtctcc ataatccacc ccgatatatt gacttgcggc
      481 ggagccttag aattgaaaaa gattgcggat attgcagatg aattcggggt tgcagtggca
      541 gtgcatatgg cggaaagtcc tatagcctgc atggcggcag ctcatgtttg tgcggcaatg
      601 cgaaatgtcc ttgcaatgga atttcactct gtggatgttc cgtggtggag cgatatagtg
      661 gtaggtttac ccaagcccat aatacaagac ggatattgta ccgtgccgga taaaccggga
      721 ctgggaatcg atgctttaaa tgaagaagtt ataaaagaac atattaaccc gcaaataccc
      781 ggaatgtggg aagaaacttc caaatgggat aaagagttct cgaatgacag aatttggagt
      841 taaagaaaat agtaatttgt aaaacaactt atagagaggt ataaaaatgc atttcaacag
      901 caaagaagac ataattgaaa tgactccctt gtggaaggga gagcgcttag aggacggcag
      961 accttatgtt gatccgaagt atttgacaga actaagaaaa atgacattgg aggaagtatg
     1021 gaagcctatc tatgtcaagg gttatgaaaa ccagtttgaa gggcgattaa aaaccttgca
     1081 tgatgacggc agaaagctta tcggaagagc ggtcacctgc acattttgtc ctacacgacc
     1141 ggatttgcat gaaataatgt ttaggatagg ttcaaacgaa ggaagaaaag gtaattacaa
     1201 tcagtgggta atcgacagcc tggttgaggg tgatgtagtt gtagcggaca tgtatgataa
     1261 ggtttataaa ggtacttttt tgggaggaaa ccttacaaca gctattaaaa acagaacaaa
     1321 aaccggaggc ggtgttatct ggggcggcat tcgcgatgta gaacagatga agcaggcgga
     1381 agggattcag gtatattatc gcggtattga ccccactcct atccgggaat gcgttatgac
     1441 aggctttaac acaccgacca gaataggtaa tgccatatgt cttcccggag atattgtttt
     1501 cggagcggga ggaggagtat tattcattcc cagccacttg gttgccgagg ttgtggaagg
     1561 tgcggctaag acacatatca aagatgaatt cggctttgag atgataaagc agaataaatt
     1621 tactacggct cagatagacc ggaatacatg gactgtggaa atgctggata tgctggtaga
     1681 ctttatacaa aatgatgagc gcggtaagaa ttatcgtgat attgattggt caaaggaata
     1741 ttacatggct aaacacggcg atccgaacga tacgcagaca gcattgtaga ggtatttgat
     1801 gaaactacgt tggatcgggc aaagcggata tattttagag gacgatacta cagaaattat
     1861 aatcgacccg tatttatcgg atattgtaaa taaagttgca aatcgcccga gattagtgcc
     1921 gtctcctata caaccggaag atattaatgc agattatgtt atctgtacac atgaccatct
     1981 ggaccacatt gatcccgaaa caattgaaag aatgaataaa gacactgttt gctttatagc
     2041 accttcttcc tgcaaagaac gtctttcggc actgggatgt aaaaatatta aaaccataga
     2101 tgcaggagaa caaatatcag ccggaaagtt ccagataaca gcggtctttg ccaatcattc
     2161 ggtagatgcc atcggaatca tagcggaaca ggattcaata aaactgtatt tttccggtga
     2221 taccttgtac catgagaaat tggaagagtt gaaagaatca gggattaccg taatgtttgt
     2281 atgtattaac ggaaaactgg gaaacatgaa tgcagacgag gcagctaagc tgacaaagat
     2341 tatcaatcct ttggtaggag taccgaccca ttacggtatg tttgcaagca atacggaaga
     2401 tcctgaaaaa tacacatcaa agattgataa cggctttatt atggagttca ataaagaata
     2461 tactatttcc gaattattaa ggggagaata aatatgcaac ataaattaag cggaaaaaga
     2521 gcactcatta ccggttccgg tcgcggcatc ggaagagcaa tcgctatcga gcttgctaaa
     2581 aacggagcgg atatcattgt acattattta ggtaatgtaa aaagtgcgga acaagttaag
     2641 aaacagattg aggcttctgg cggctcctgt tcaatcatac aagcggattt atgcaaaccg
     2701 gaaacattag aaggtttggt cgaaaaaata ggcggtgttg atattttagt gttgaatgca
     2761 tcggttcaat acagaagaca atgggaaaac atatcacttg aggaatttta cgatcagatt
     2821 aattgcaatc tgtgtgcaag catgttactt attcagcaag cagtaccgta tatgcgtaaa
     2881 aaacattggg gaagaataat aacgatagga agtgtacagg aagcaaaacc gcatccggac
     2941 atgttggttt attcggcatc taaagccgcg caaaccaata tggtaaaatc cttggcggtt
     3001 cagttggcaa aagacggaat cactgttaac aatgttgcac cgggtgctat taataccgac
     3061 cgcaacaagg atgttttgaa agataaagaa tattataaaa aagtgacaga ttccattccg
     3121 gccggcttta tcggtgaacc cgccgactgt gccggtattg tatctttact ttgttcggaa
     3181 gaaggaaggt acatcacagg tcagagtata tatatagacg gcgggaaatg cctgtaattt
     3241 gaaaggagca agtaatgagt catacattat taagattttt tgaagaacag aaagtacaga
     3301 gcaacaaaag aatagaaggc gatattcaaa aataccgtaa agcctttgca aaaataaagg
     3361 tagttaacag ccaaggagat ttaatagaaa atgttaaagg tcgcataaag caaaaaacgc
     3421 atgattttaa gttcgggtgt aatatattta tgctggatca attcccggac gaggaacata
     3481 ataaagcgta cagaaaagag tttaaaaagc tttttaacta taccgttgca ccgttttatt
     3541 ggcaggattt tgagttgaaa gacggagaac cgagattagg gaaaaatccg gttaatgttt
     3601 atcgcagacc gtctattgaa aagatattga aatattgcgg ggagaataac attgatgtaa
     3661 aaggacaccc gttattctgg catctgtttt taccccaatg gctcgtgcag gacaagaatg
     3721 aagtatacta tcggatagaa cgcagacttt ttgagatagc agaggtattt aaagaccgta
     3781 ttcgtgattg ggacgttgta aatgaatcat tatcccgtcc tcctcaccgg gaaaattatc
     3841 agcttcccta tgactatgta aaccgcatct ttgatttagc ggacagatat tttcccaaga
     3901 accgtctttt tataaacgac acg
//
LOCUS       DAOPWC010000097         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_23410_length_3919_cov_2.463768, whole genome shotgun sequence.
ACCESSION   DAOPWC010000097 DAOPWC010000000
VERSION     DAOPWC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23410_length_3919_cov_2.463768"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKJ65_03035"
     CDS             <1..1096
                     /locus_tag="PKJ65_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ40846.1"
                     /translation="SELNKICIKNKDIGFISSGIAFQYAREALPDASYLKLGITYPIS
                     EELLKKFASSVKKIYVIEELEPIIETACKACGIEVTGKQLLPVTGEYSVELLRNRLFG
                     QKPYNCMEGYDMSKVPGRPPVMCPGCPHRGIFYVLKRLKLTVTGDIGCYTLAALQPLA
                     AMDTSICMGSGITSLHGFNKARGDEYMKKCVGVIGDSTFVHSGITGLIDIVYNKGVGT
                     VIVLDNSITGMTGHQNNPLNGLDIKNNPAKQIDLVMLAKSIGIERVRIIDPFDVEETM
                     AVIKEEVNAMEPSLIISQRPCALLKTVRYKGCVTVDRDKCTKCKMCMSLGCPAISFKN
                     NQIEIDTSLCNGCGLCVNVCRFGALKKEEK"
     gene            1096..1671
                     /locus_tag="PKJ65_03040"
     CDS             1096..1671
                     /locus_tag="PKJ65_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNZ40847.1"
                     /translation="MVKSIVIAGVGGQGTLFASTLIGEAATLKGFDVKVSEVHGMAQR
                     GGSVITYVRYGKQVISPIIPVGEADVLIAFEQLEALRWAHYLKKGGILIGNTQKIDPM
                     PVITSSMQYPDDIIPELTGRIDNCIFMDASELAEQSGSIRTVNSVLIGRMARDSEIEK
                     EIWLEALKKTAKPAFIDMNIKAFEAGYNYGV"
     gene            1762..3879
                     /locus_tag="PKJ65_03045"
     CDS             1762..3879
                     /locus_tag="PKJ65_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40848.1"
                     /translation="MSITEKDKIFEQDRLNEVYAKLSKRIEDLEVSLKQSIDSLKKSN
                     QMMWESGKRNPADFHDAIENMPYLESIKYDFFKTETIKKELRRMFLLQRSMYFGRIDF
                     QEQDQEDVEKIYIGKFSLTDDEGQYLVFDWRANICSLFYENELGIVFYNAPDGRIYGD
                     MSKKRQFDILYDNINAMFDSSLMIDDDILKDILSKSKDSKMSNIVETIQKEQNKAIRS
                     TYSAIIVDGPAGCGKTSIAMHRAAWLMYKYKNELQNNQIIIFSPNETFNDYISEVLPE
                     LGEENIRMSTFRNLAYHILSDRRVLISKYVELEDVLLDNEYADSDVLRLKYSVDFANK
                     LKSYTELISEGDYPFKDLIVDGITIFTKREFEDLFYNEYSFNDVMTRLAKIRIVLNER
                     IFPVIRNLRLEYVRKVKADELDMSKGFKELRESCKRIYQAVDMSVSADFEMIYAQFLK
                     HSYSAKISSDFLERLLDGYIYAEDTAPLIYLRLLLGNRYNFSDIRHVIIDEFQDYSYI
                     EKLVISLLFGKCHMTLLGDINQKINHYQEKVNDKDIFITARSVKLDKSYRSTYQIAKF
                     CNDMLKDNPDIKYFNRQGKQPEVLSLSTDNMDVIANTVINKVSEYRASGHRSLAVITR
                     TEWFAKKLHPYLKDAKINRVTEKSTSYTYGAVLIPSYLTKGLEFDAVIVLDTDIDKFK
                     KEEELHLFYTCCTRALHELTVIN"
BASE COUNT         1307 a          655 c          882 g         1075 t
ORIGIN      
        1 gtcggaattg aataagattt gtataaagaa taaggatata ggcttcatat cttcgggaat
       61 agctttccaa tatgcccgtg aagctctgcc cgatgcatct tatctgaaac tgggtattac
      121 ttatcccata tccgaagagt tattaaagaa attcgcttca tcggttaaga agatttatgt
      181 tatagaagag ttggaaccga taattgaaac ggcatgtaag gcatgcggta tagaggtgac
      241 ggggaagcaa ctgctgcctg taaccggaga atattcggtg gaacttttga gaaacaggtt
      301 attcggtcaa aagccgtata actgcatgga aggctatgat atgagcaagg tgcccggcag
      361 acctcccgta atgtgcccgg gatgtcctca cagaggaata ttttatgttt taaagagact
      421 taaactgacg gtaacgggcg atatagggtg ttacacttta gcagctttgc aaccgcttgc
      481 cgctatggat acttccattt gtatgggaag cggtattact tcacttcacg gcttcaataa
      541 agcaagaggg gatgagtata tgaagaaatg cgtgggtgtg ataggagatt ccacattcgt
      601 gcattcgggt attaccggac ttatagacat agtctacaac aaaggggtag gcaccgtaat
      661 cgtattggat aattccataa ccggtatgac gggacatcag aataatcctc tgaacggatt
      721 ggatattaaa aataatcccg ccaaacagat tgatctggtt atgctggcaa agagcatagg
      781 tatcgaaagg gtaagaataa tcgatccttt tgatgtggaa gaaacgatgg cggttattaa
      841 agaggaggtc aatgcaatgg aaccttctct gataatctca caaagacctt gtgcattgct
      901 taaaacggtc aggtacaaag gatgcgttac cgtagacaga gacaaatgta caaaatgcaa
      961 aatgtgtatg agtctgggat gtcccgcgat atctttcaag aataatcaaa tagaaataga
     1021 tacatcctta tgtaacgggt gcggtttatg cgtcaatgta tgcagattcg gtgcattgaa
     1081 aaaggaggag aagtaatggt aaagtcaatt gtaatagccg gagtgggcgg acagggtaca
     1141 ctatttgctt ctacactgat aggtgaagcc gccacattga aaggttttga tgtaaaagtt
     1201 tccgaagtgc acggcatggc gcaaagaggc ggctcggtta taacatatgt acgttacggg
     1261 aagcaagtca tatctcccat aatacctgtt ggagaagccg atgtacttat tgcttttgaa
     1321 cagctggaag ctctgagatg ggctcattat cttaaaaaag gcggtattct tataggtaat
     1381 acacaaaaga tagatcctat gcctgtaatt acatcttcga tgcaatatcc cgatgacatt
     1441 attccggaac taaccggaag aatagataac tgtatcttta tggatgcttc ggaattggct
     1501 gaacaatcgg gttccataag aacggtaaac tcggttctga taggtcgcat ggcaagagat
     1561 tccgagatag agaaagaaat atggttggaa gctttgaaaa agaccgcaaa acccgctttt
     1621 atcgatatga atataaaggc atttgaagcc ggatacaact acggtgtctg actctatccg
     1681 ttaaacttcc cgtttgctat atgagcaaaa ctaaggtata attatggtta atgcatattg
     1741 tatctttagc aaggggcgta tatgagcatc actgaaaagg acaagatatt cgaacaggat
     1801 agattgaatg aggtctatgc caagctgtca aaaaggattg aggatcttga ggtatcttta
     1861 aaacaaagca ttgattcttt aaaaaaaagt aatcagatga tgtgggaaag cggaaagcgt
     1921 aatcccgcgg attttcatga tgccatagaa aatatgcctt atctggaaag cataaaatac
     1981 gacttcttca aaaccgaaac cataaaaaaa gaattaagaa ggatgttcct gcttcagaga
     2041 agtatgtatt tcggcagaat agattttcag gaacaggatc aagaggatgt agaaaagata
     2101 tatataggaa agttcagtct gaccgatgat gaagggcagt atctggtttt tgactggaga
     2161 gcaaatatat gcagtctgtt ctatgaaaac gaattgggaa ttgtctttta taatgctccc
     2221 gacggcagaa tttacggaga tatgtctaaa aagcgacagt ttgacatatt gtatgacaat
     2281 ataaatgcta tgttcgattc gtcattaatg attgacgatg atatcttaaa agatatcttg
     2341 agtaagtcca aagactcgaa aatgagtaat attgtggaaa caatacagaa agaacagaat
     2401 aaagccataa ggagcactta cagtgcaatc atagtggacg gaccggcagg ctgcggtaag
     2461 acttccatag ccatgcacag agccgcctgg ctgatgtata aatataaaaa tgaattgcag
     2521 aataatcaga taatcatatt ctcgcccaat gaaacattta acgactatat ttcggaagta
     2581 ttgccggagt tgggtgaaga gaatataagg atgtctacat tcagaaatct ggcatatcat
     2641 atcttatccg acagaagagt gcttatatcc aagtatgtgg aattggagga tgttcttttg
     2701 gataacgaat atgcggattc ggatgtttta agacttaagt attccgttga ttttgcaaac
     2761 aaacttaaga gttatacgga acttatctcc gaaggcgact atccttttaa agaccttatt
     2821 gttgacggaa ttactatttt tacaaaacgg gagtttgaag atctgttcta taacgaatat
     2881 tcttttaacg atgtaatgac cagactggct aagataagga tagtgcttaa tgaacggata
     2941 ttcccggtta tccgaaactt aagattggaa tatgtacgta aagttaaagc cgatgaactg
     3001 gatatgtcga aaggattcaa agagttaaga gagagttgca aaaggattta tcaggctgtc
     3061 gatatgtcgg tatcggcgga ttttgaaatg atttatgcac aattcctgaa gcacagctat
     3121 tcggcaaaga taagttcgga ttttttggaa agacttcttg acggttacat atatgcagag
     3181 gatacggcac cgttaatcta tttgcgactg ctcttgggta acagatataa tttcagtgat
     3241 ataaggcatg taataatcga tgaatttcag gattattcct atattgaaaa gcttgtcata
     3301 tcattactct tcggaaaatg ccatatgact ttactgggtg atatcaatca gaaaattaat
     3361 cactaccagg aaaaagtcaa tgataaggat attttcatca ctgccagatc ggtaaaactg
     3421 gacaagagtt atcgttccac atatcagatt gcaaaattct gcaacgatat gctgaaagac
     3481 aatcccgata taaaatattt caacaggcag gggaaacaac cggaagtttt aagcttaagt
     3541 accgataaca tggatgtaat agcaaatacg gtgattaata aggtatcgga gtacagagca
     3601 tcaggtcatc gttccctcgc cgttataacc agaaccgaat ggtttgccaa gaaacttcat
     3661 ccctatctca aggatgcgaa aatcaacaga gttaccgaaa agagcacatc ctatacatac
     3721 ggtgcggtgc ttataccgtc ttatcttaca aaagggctgg agtttgatgc ggtaatcgta
     3781 ctggataccg atattgacaa gtttaaaaaa gaagaggaat tacacctctt ttatacctgc
     3841 tgtacaagag ctcttcacga actcaccgta ataaactaac ttaaatcata ttctaacaca
     3901 cttcataaac atgcttcat
//
LOCUS       DAOPWC010000098         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_23437_length_3915_cov_2.693523, whole genome shotgun sequence.
ACCESSION   DAOPWC010000098 DAOPWC010000000
VERSION     DAOPWC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23437_length_3915_cov_2.693523"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKJ65_03050"
     CDS             <1..528
                     /locus_tag="PKJ65_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNZ40849.1"
                     /translation="PDIKKDSKEVSIILEKLKEMEHYGYQFEAADASFELLVKRTLGL
                     YKPHFNLVLYKTMSEFPVPAGGMPASATIKVEVDGKEEITAVMGNGPVNALDLALRKS
                     LTVFFPAIKEMQLMDYKVRVLEENSTTAAKVRVLIETSDGKKSWSTIGVSNDIIEASL
                     IALADSIEYKLSMNE"
     gene            complement(531..881)
                     /locus_tag="PKJ65_03055"
     CDS             complement(531..881)
                     /locus_tag="PKJ65_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ40850.1"
                     /translation="MNCPKCGTLLKENAKFCSGCGAQVPQVQSASNSCAYCGATLVAG
                     AKFCAYCGAAAVSSSNACPRCNTPFKPGAKFCGVCGAPATTSTLPPLNVCKNCGTPFK
                     PGTRFCGKCGSAIL"
     gene            complement(1078..2001)
                     /locus_tag="PKJ65_03060"
     CDS             complement(1078..2001)
                     /locus_tag="PKJ65_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40851.1"
                     /translation="MPLVAAKCTQCGANLQIDSSKDAAICPNCNTPFVTEKAITNYKT
                     YYEYKIEKADVHIHDEKSVETRLKNAEIFFKKHNNIDKAYELFHSVANDAPGDYRGWW
                     GLVRVKTNDFDSPEISRKETDDIKYYANCAFNVAPSDMLDKLEQTWRTYNQQVYKFHS
                     KLSLDKEEWVNQLLTAQANILSLESRITLLSNEIIESDIICKRRNDSKLFYFIPTAII
                     LGVISLIGLLTNIFSKEGESSILLPLLGLLYSAILAAVYVIFKCIKKNAEQLNQEKKK
                     QKEKLIDTVNEYHKTKTTLLEKISFAEKILS"
     gene            complement(2171..3517)
                     /locus_tag="PKJ65_03065"
     CDS             complement(2171..3517)
                     /locus_tag="PKJ65_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNZ40852.1"
                     /translation="MFKLKENGTKVSTEILAGLTTFVTMAYIIAVNPGMLSLAGMEWG
                     AVFLATIIASIIGTLIMGLVANVPYAQAPGMGLNALFVFTVCFTYGFSWQHALSMVFI
                     CGVINILITVTKIRKHIIKAIPKSLQNAIGGGIGIFIAYIGLLNVGAINFGAGVPSMS
                     VINTGAIYVFIIGLVLTIVLLLLKVKGAILIGIVAATLIGIPLGVTTSADKVSFIDAV
                     NVLPTTLGVIFTKEGLGSLFSDVSKLPIVLITIFAMSLSDTFDTIGTFIGTGRQTGIF
                     SEEDEKAMESSSGFKSKMDKALFADATATSIGAIFGTSNTTTYVESAAGIAVGGRTGL
                     VSVVVAICFALSAFFASFISSIPGAATAPALVVVGIMMLSSFKEIQWSELEEAIPAFF
                     AGIFMALAYSITTGIAMGFIFYLIVKVCKRQFKDISPVLWVSSALFILNFVLQAVV"
BASE COUNT         1185 a          761 c          784 g         1185 t
ORIGIN      
        1 cccgatatta aaaaagactc aaaggaagta tcgataatat tggagaaact caaggaaatg
       61 gagcattacg ggtatcagtt tgaagctgcg gatgcatctt ttgaattgct tgtcaaaagg
      121 accttaggat tgtataaacc tcattttaat ctggttttgt ataagacaat gagcgaattt
      181 cccgtacctg cgggcggtat gcccgcttcg gcgaccataa aagtggaagt tgacggcaaa
      241 gaagagataa cggcggtaat gggaaacggt ccggttaacg cattggatct ggcattgaga
      301 aagtcgttga cagtattttt cccggccata aaagaaatgc agctcatgga ctataaggtg
      361 cgagtactgg aggaaaacag cactacagcg gctaaagtaa gggtgcttat cgaaacatcc
      421 gacggcaaga agagttggtc gacaatagga gtatccaacg acataattga agcaagcctt
      481 atagctttgg cggactcgat tgaatataag ctttcgatga acgaataata ttataaaata
      541 gcactaccgc attttccgca gaatctggtg cccggtttaa agggtgtgcc gcagtttttg
      601 catacattta aaggcggtaa tgttgaagtt gttgcaggtg ctccgcatac tccgcagaac
      661 ttggctcccg gtttgaacgg ggtattgcat ctgggacaag cattggagga tgaaaccgca
      721 gcagcaccgc agtaagcaca gaatttagct cccgctacaa gtgtcgcacc gcaatatgca
      781 caggaattgg aagcactctg tacttgggga acctgcgcac cgcaacccga acaaaactta
      841 gcattttctt tcaataatgt accgcatttt ggacaattca taagctcccc cttgtaatca
      901 aaaacattct tgacacaatt attttaacag aatatattcc gaattcaata tgcatagaga
      961 aataatgaaa gtttcaagat gcactctgtt ctgtaataac cctgtttttg tgcatttata
     1021 agatgctgaa gagtaaagct ttatcggatt tatgctgtat ataagatatg taaaggacta
     1081 actcaatatt ttttccgcaa aagatatttt ctctaagagt gttgtttttg tcttgtgata
     1141 ttcgttaacg gtgtctataa gcttttcttt ctgtttcttc ttttcttgat taagctgttc
     1201 cgcatttttt ttgatacatt tgaatataac atatactgca gctaaaattg ctgaatagag
     1261 taagccaagt aatgggagaa gtatagagga ttctccttct ttactgaata tattagtgag
     1321 taaacctatc aaggaaatga cacctagtat aatggccgta ggaataaaat aaaagagttt
     1381 gctgtcatta cgtctcttac atataatatc agactctatt atttcattag acagcagggt
     1441 aattctgctt tctagagata atatgtttgc ttgagcggta agtaattgat taacccattc
     1501 ctctttgtcc aaagacagtt tgctgtgaaa cttataaacc tgctgattat aggttctcca
     1561 cgtctgctcc aacttgtcca gcatatcgga gggagcgaca ttaaatgcgc agttggcata
     1621 atatttaatg tcatctgttt cctttctgct gatttcagga gaatcaaaat catttgtttt
     1681 tacgcgaacc aatccccacc agcccctgta atcaccggga gcatcgttgg caacactgtg
     1741 gaacagctca tatgctttgt caatattatt gtgctttttg aaaaatatct ctgcattttt
     1801 aagacgtgtc tcaacggatt tctcatcatg tatatgcaca tcggcttttt ctattttata
     1861 ttcatagtat gttttgtaat ttgttattgc tttttcagtt acaaaggggg tattgcaatt
     1921 tgggcatatc gctgcatcct tggaactgtc aatttgcaga ttcgcaccgc attgggtgca
     1981 ttttgcggct actagcggca taggaaactc ctttatttat tattttgagt gtatacaatt
     2041 gctgtcaaat tgtcaacaaa aagccactgt tgtaatgact gcctattgtc tgttttaata
     2101 atgtgaaaat gtgttattaa aaaaagaacc gctctgctga acggctcttt cggttgttat
     2161 ctttcgtttt ttatactact gcctgtaata caaagttcag tatgaacaag gctgaagaaa
     2221 cccataatac cggtgatatg tctttaaact gtctcttgca aactttgact atcaggtaga
     2281 aaatgaagcc cattgcaata cccgttgtga tggagtaagc aagtgccatg aatatacctg
     2341 caaagaaagc cggtattgct tcttccagtt ccgaccattg aatttccttg aatgaggaaa
     2401 gcatcattat acctactacc acaagtgcgg gagcagttgc cgctccggga atggaactga
     2461 tgaaggaagc aaagaatgca cttaatgcaa aacaaattgc aactacaaca ctgacaagac
     2521 cggtacgtcc gccgactgct atacctgctg cactttcaac ataagtagta gtattggatg
     2581 tgccgaatat agcaccgatg gatgttgcgg ttgcgtctgc aaataaagct ttgtccattt
     2641 tggatttgaa tcccgagctg gattccattg ctttttcgtc ttcttcgctg aatattcccg
     2701 tctgacgtcc cgttccgata aatgttccta tcgtgtcgaa agtatcggat aagctcattg
     2761 cgaaaattgt aataagaact atgggcaatt ttgaaacatc ggagaacagt gaacccagtc
     2821 cctcttttgt gaatataacg ccgagtgttg taggcaacac attaacggca tcaatgaaac
     2881 ttactttgtc tgcagatgtt gttactccta acggaatacc gataagtgtt gcggctacta
     2941 ttccgataag tattgcaccc tttaccttga gcagaaggag tactatcgta agaacaagac
     3001 ctataataaa tacatatata gcgcccgtat taataaccga catggacggt actccggcac
     3061 cgaaattaat ggcacctaca ttcaataatc cgatgtaagc gatgaatatt ccgataccgc
     3121 cgccgatagc attctgaagg ctcttcggta ttgcttttat tatatgctta cgaattttag
     3181 ttaccgtgat aagtatgttt attactccgc agatgaaaac catggacaat gcatgttgcc
     3241 atgaaaaacc gtaagtaaaa catacggtaa atacgaacag agcgtttaac cccattccgg
     3301 gagcctgtgc atacggaaca ttggcaacaa gacccattat cagtgtaccg attatcgacg
     3361 caataatggt tgcaaggaaa acagctcccc attccattcc tgccagcgat aacattcccg
     3421 ggttaacagc gataatatat gccattgtaa cgaatgttgt cagacccgcc aaaatctctg
     3481 ttgacacttt tgtgccattt tctttgagct tgaacatgtt taaatccccc ttttgaattt
     3541 aatatactgc aagtatatta catggtgata aattgtcaat ataaaagacg aaaaaataac
     3601 tcgaatttat taaattccac aacatgttgt tatatgacat aatcaaaaac gatacatatt
     3661 gatttttgct gtactcactg aaatttacgg ttgtacagag gatttttctt gaaaaaattt
     3721 ttgaaaacag aaaaagaccg taactcctgt gaagttacgg ccttaggttt ttacaacaaa
     3781 tcggattatt gtcctcttgc gttaaagtcc tgacagatat agttcattgc aatggaaagt
     3841 ctatgcaaat aaatgaaagg tcctactatg ataagcgatc ctaatacacc ccaaagccaa
     3901 tatgtattgg aattg
//
LOCUS       DAOPWC010000099         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24026_length_3844_cov_4.082080, whole genome shotgun sequence.
ACCESSION   DAOPWC010000099 DAOPWC010000000
VERSION     DAOPWC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24026_length_3844_cov_4.082080"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKJ65_03070"
     CDS             <1..451
                     /locus_tag="PKJ65_03070"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNZ40853.1"
                     /translation="RLASKADAVVPNITEACFMLDKKYKEGPYDEQYIRDLLLGLADM
                     GPDKVVLTGVYYNEKELGAATYDKTIDQFGFYKRERIEGYYHGTGDVFGSALLAAMIK
                     GKNLLDSATVAVDFTVESLIRSYKEKTDIRFGVNFEAGLEKLAKVLK"
     gene            complement(465..2141)
                     /locus_tag="PKJ65_03075"
     CDS             complement(465..2141)
                     /locus_tag="PKJ65_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40854.1"
                     /translation="MRRRVFISIFLTSLLTMILTFVLISWIMYNNTFDSIKREVKNES
                     FYVSDALKTYDDDSPDMYSYLNKVGLNSSNRITYIKNNGTVLYDNLAQASDLDNHSDR
                     PEVIEALQNGSGEITRYSDTLGESIYYYALKLGNGNILRIAATTKSILGIMIGSAIWI
                     IIIFITFISLSVIIARLLTKSIVKPINTLDLDNPLSNKTYDELSPLLLRLDKQKTELM
                     KNVQEMSKARINFAFISNNMSEGIVVFSEKGYITGANKSAREILGISIQQFFMEGCRD
                     LDYIKAVRSALDGKPMTTKFEKNGLVYQLSASPVKENPEKHAALMFITDITEKDQAEQ
                     RRKEFSGNVSHELKTPLTSISGYAELISKKIAKKEDVPRFAEMIHTEAIRLLQVIEDI
                     MKLSRLDESDLEKEFEYVSLKKICSSVVNDLTPIAQHKNVAINTHLKDVYINGYAPIL
                     YEMLYNLIDNAIIYNNPKGHVDISLAKEEDEIILSVKDNGIGIPPEHQPRIFERFYRV
                     DKSHSKDTGGTGLGLSIVKHAAALHKADIDLISAPGEGTTVMITFYDSDK"
     gene            complement(2138..2824)
                     /locus_tag="PKJ65_03080"
     CDS             complement(2138..2824)
                     /locus_tag="PKJ65_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ40855.1"
                     /translation="MIYFVEDDSSIRELVVYTLNNTGFEAEGMDNAKMLYSKISVNKP
                     ELILLDIMLPGESGLEILKKLRSSKETAHIPIIMVTAKGTEYDKIIGLDSGADDYISK
                     PFGIMELVSRVKALLRRVSRKDDTDSETDTYVYEGVRINSKNHTVTLDSEPVVLTLKE
                     FRLLYELMANQGIVLTRDSLLERVWGYDYPGETRTIDVHIRTLRKKLGKYGDIIKTIR
                     GVGYIIGGNA"
     gene            complement(2827..3471)
                     /gene="phoU"
                     /locus_tag="PKJ65_03085"
     CDS             complement(2827..3471)
                     /gene="phoU"
                     /locus_tag="PKJ65_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNZ40856.1"
                     /translation="MRSKFDMQLEILNNSLIEMGALCEKAIATAIKALLENNIPLAKK
                     VMESVKDINQAEKDIEAMCLKLLLQQQPVARDLRQISSILKMITDMDRIGVQAGDIAE
                     IVTFANLSKSKDRVHIADMAEATIKMVSESINAFVKKDLELANKVIAYDDVVDDLFVK
                     VRTGIVDLISKDKRHGEQAVDIIMIAKYLERIGDHATNIAGWVAFSITGIHVEL"
     gene            complement(3471..>3844)
                     /locus_tag="PKJ65_03090"
     CDS             complement(3471..>3844)
                     /locus_tag="PKJ65_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ40857.1"
                     /translation="TDAAIFNEVKDRLKRNALELSGGQQQRLCIARALAVEPDVLLMD
                     EPTSALDPISTSKIEDLVLELKNRYTIIVVTHNMQQATRISDKTAFFLLGEVIEMNDT
                     GILFSNPADVRTENYITGRFG"
BASE COUNT         1024 a          815 c          730 g         1275 t
ORIGIN      
        1 aagacttgct tccaaggccg atgcggttgt tcccaatatt accgaagcct gttttatgct
       61 ggataagaaa tataaagaag gaccgtatga tgaacaatac atcagggatt tgttattggg
      121 tcttgcagac atgggtcccg ataaagtggt tctgacaggt gtctattata atgaaaagga
      181 actgggtgcg gctacatatg ataagaccat tgatcaattc ggattctata aaagagagcg
      241 tatagaggga tattatcacg gaaccggtga tgttttcggc agcgcattgt tggcagcaat
      301 gattaaagga aagaacctgc tggattccgc aacggtagca gtggatttta cggtagaaag
      361 tcttatccgt tcatataaag aaaaaaccga catacggttc ggtgtaaact ttgaagcagg
      421 tcttgaaaaa ctggcaaaag tattaaaata agaagtcgtt tatattactt atcactgtca
      481 taaaaagtaa tcattaccgt tgtaccttca cccggagcac ttattaaatc aatatccgct
      541 ttatgtaatg ctgcggcatg ttttacgatg gataaaccca gtcccgttcc gcccgtgtct
      601 tttgaatggc ttttgtccac tctgtagaag cgttcaaata ttctgggctg atgttccggc
      661 ggaataccaa tgccgttatc tttaacggaa agaatgattt catcttcttc ttttgctaag
      721 gaaatatcaa catgcccctt aggattattg tagattatgg cattatcaat cagattgtac
      781 agcatttcat aaagtatcgg agcataaccg tttatataca catctttcaa atgtgtgttt
      841 atcgctacat tcttatgttg cgctatcggt gtcaaatcat taacaacaga ggaacaaatc
      901 tttttcaggc tgacgtactc aaattccttc tccagatccg attcatccag tcgggataac
      961 ttcataatat cttcaataac ctgaagcagt cttatagcct cggtgtgtat catttccgca
     1021 aaacggggca catcctcttt ttttgcaatc tttttcgata tgagttcggc ataaccggat
     1081 atggaagtaa gaggtgtctt cagctcatgc gagacattgc cggaaaattc ttttcttctc
     1141 tgttcggcct gatccttttc cgttatatcg gtgataaaca taagtgcggc atgtttttcg
     1201 ggattttcct ttaccggact tgccgaaagc tgatatacca atccgttttt ttcgaatttt
     1261 gtcgtcatcg gttttccgtc aagtgcagag cgaaccgctt tgatataatc gaggtctctg
     1321 caaccttcca tgaagaattg ctgtatactt atgccgagta tttctctggc gcttttattt
     1381 gcaccggtta tatatccttt ttcggaaaac accacgattc cttcggacat attattggat
     1441 atgaaagcaa aattaatgcg ggctttggac atttcctgca catttttcat taattcggtt
     1501 ttctgtttat ccaatcttaa gagcaaagga gataactcat cataagtttt gtttgataac
     1561 ggattatcca gatccagagt attaataggt ttgacaatgg attttgtcaa cagtcttgct
     1621 atgattacag acagtgatat gaaagtaatg aatatgatga taatccatat cgcggagcct
     1681 atcattattc ctaaaatgct tttggttgta gcggcaatac gtaaaatgtt accgtttccc
     1741 aacttcaacg cataatagta tatgctttct cccaatgtat cggaatatcg ggtaatctca
     1801 ccggacccgt tttgtaaagc ctctattacc tccggtctgt ccgagtgatt atccaaatcc
     1861 gaggcctggg ctaaattgtc ataaagaacg gtcccgttat tctttatata tgttattctg
     1921 ttggaactgt taagccccac tttgttaaga tacgagtaca tgtcgggaga atcatcgtca
     1981 tacgttttca gagcatccga aacataaaaa gattcgtttt taacctcgcg ctttatgctg
     2041 tcgaaagtgt tattgtacat tatccaggag ataaggacaa aggtaagtat catggtcagc
     2101 aacgaggtca ggaagatgct tatgaaaacc cgtcttctca tgcattgcct ccgataatat
     2161 atcctacgcc tcgtattgtt tttataatat ccccgtattt tcctaatttt ttcctcagtg
     2221 tccgtatatg tacgtcaatt gtccttgttt cgccgggata atcataaccc catactctct
     2281 ccagcagaga atcacgtgtc aggactattc cctgattagc cataagttca tataaaagtc
     2341 tgaactcttt gagagtcaga accacaggtt ccgaatccaa tgtaacggta tggttcttgc
     2401 tgtttatgcg cactccttca tatacataag tatcggtctc actgtcggta tcatcttttc
     2461 tggatactct tcgtaaaagg gcttttactc tggataccag ttccattata ccgaagggtt
     2521 tggatatgta atcatcggcg ccgctgtcca gtccgattat cttgtcatac tcggtacctt
     2581 ttgcagttac cattattatc gggatatgcg ccgtttcttt tgaagaacgt aattttttca
     2641 gtatctccaa tccgctctcc ccgggtaaca taatgtccaa cagtataagc tccggtttat
     2701 ttaccgatat tttactgtac aacatttttg cattatccat gccctctgct tcaaaacccg
     2761 tgttgttaag ggtatatact acaagttccc gtatactgct gtcatcttcc acaaaataaa
     2821 tcatttttac agttccacat gtatgccggt aatggagaaa gcgacccatc cggcaatatt
     2881 tgttgcgtgg tctcctattc tttccaagta tttagctatc attattatat caaccgcctg
     2941 ttctccgtgt ctcttgtctt ttgatataag atcgacaatg ccggttctga ctttaacaaa
     3001 aaggtcatct accacatcat cgtaagctat tactttattg gcaagttcaa gatccttctt
     3061 aacaaatgcg tttatgcttt ccgaaaccat ttttatggtt gcctcagcca tatcggctat
     3121 atgtactctg tccttgcttt tagacagatt ggcaaaagta actatctcgg caatgtctcc
     3181 ggcctgtact ccgattctgt ccatgtccgt tatcatttta agtattgagg atatctgacg
     3241 taaatctctt gcaacgggtt gttgctgaag aagcagtttc aggcacattg cttctatatc
     3301 cttttcagcc tgatttatat ccttaaccga ttccattact ttttttgcaa gaggaatatt
     3361 attctcaagt aaagccttta tagccgttgc aatggctttc tcacataatg cacccatctc
     3421 tataaggctg ttatttaata tttctaactg catatcaaat ttactgcgca tcatccgaac
     3481 ctccctgtta tgtagttttc agtacgcaca tcagccgggt ttgagaataa aattcccgta
     3541 tcgttcattt ctattacctc accgagaagg aaaaatgccg tcttatcgga tattctggtt
     3601 gcctgttgca tgttgtgtgt gactactata atagtatatc tgttttttaa ttccagtaca
     3661 aggtcctcga ttttagatgt ggatatgggg tccaatgcac ttgtaggttc gtccatcagc
     3721 agaacatccg gctccacggc caatgcacgt gcaatacaca gtctctgttg ctgaccgccc
     3781 gagagttcca atgcatttct tttcagacgg tcttttactt cattaaaaat agccgcatcg
     3841 gtca
//
LOCUS       DAOPWC010000100         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24091_length_3836_cov_3.667548, whole genome shotgun sequence.
ACCESSION   DAOPWC010000100 DAOPWC010000000
VERSION     DAOPWC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24091_length_3836_cov_3.667548"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKJ65_03095"
     CDS             complement(<1..1268)
                     /locus_tag="PKJ65_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40858.1"
                     /translation="MKITELFSDVITEDMECEFKAVLNSDNPVKWAKSIVGFANGNGG
                     MLFVGVSEDREAFGISLDEIDKTKNLVAKINDRHIFPHVKLRYLMRNVDNAAEKFVLA
                     INISPSESIVRYREGDFNETVYIKGNGYSAPATPEEIITLSKRKFGVDNEISETLYAE
                     KQWTSYAELCREFRKDGSVPTVKELQNYEIVSKDGYAKTGFLMFRDGYDGDDTLICCR
                     LWRGKTKVGEVLDNGRFKGPLPDVFNETLRFIERNTKTGWHKTENGGREEIRSYPKKA
                     ICEALVNAIAHRDYSIYGTQIDVDIYSDRIDIVSPGSWLLPKSYEEYPIGSIPSVRRN
                     TIISACLDVANLMERGGTGFQAIVDGYKDSSQDKQPCVLIYPGFLNLRLFDLLYKEEF
                     SYELLTDTEKVIGLLRDAPMPVKDLQAVTK"
     gene            1608..2291
                     /locus_tag="PKJ65_03100"
     CDS             1608..2291
                     /locus_tag="PKJ65_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNZ40859.1"
                     /translation="MKKIYTGKTKDVYCLEDGNYLLKFKDDVTGTDGVFDPGANTVGL
                     KMEGSGKAGLKLTKFFFEKLNSLGIPTHYISADIDNNTMTVKPAKMFGKGVEVILRYR
                     AVGSFYRRYGMYCTEGQHLDGYVEVTLKDDDRNDPLITDEALQMLKIMTKEEYRILVE
                     LTRKTGSIVKEEMLKKGLELYDIKLEFGRVGTDNHIALIDEISGGNMRAYCKGKYVEP
                     LELEKIMLK"
     gene            2328..2822
                     /gene="mscL"
                     /locus_tag="PKJ65_03105"
     CDS             2328..2822
                     /gene="mscL"
                     /locus_tag="PKJ65_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNZ40860.1"
                     /translation="MKKRVKRVKKEKVKLFEEFKKFITRGNVIDLAVGILIGSAFTKI
                     VSSLANDIIVPLVGLIMGKINLKELKWVLTYDAEGKALSSVNYGMFIQYIVDFLIMAV
                     CVFMLVRLFTALRKKSEELKEKAKKAEEAAAAATPPPPPAPPEPTKEQLLLTEIRDIL
                     KAQK"
     gene            2819..3808
                     /gene="corA"
                     /locus_tag="PKJ65_03110"
     CDS             2819..3808
                     /gene="corA"
                     /locus_tag="PKJ65_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ40861.1"
                     /translation="MSENNLQKPNITVTEYNSDEIREYSTDSLETLKKDVKGVRWIDI
                     DNECGKVLCEQMSRLYNIHPVVLESIYEKDQSPKLDDYQDFLFAVATMIYKKEQALVA
                     EGISFLLGDNYVISIGNLDGDVFDKVRKELHTPKSRIRMSGPDYLLYRLMDSITDNYF
                     ATLYEIEERIYRLEEEIIKDPSDSLLAKIREVKSDNQLVAKSIIPFKESRSILINMPS
                     KFITSSVAPYMNDVYDHIKEAIGLSETCRVMINDLMQLFYASTSYKLNEIIKVLTVIS
                     TIFIPLTFIVGLYGMNFKHIPEFNIPWAYPVLLGVMLVITLFMIYYFKKKKWF"
BASE COUNT         1232 a          775 c          744 g         1085 t
ORIGIN      
        1 aatttggtca ctgcctgaag gtcctttacc ggcatcggag catctcttaa caatcctatt
       61 actttctcgg tatcggtcaa tagctcataa gaaaactcct ctttgtaaag aaggtcaaac
      121 aaacgcaaat tcagaaatcc gggatatatc aatacacaag gctgcttgtc ctgactgctg
      181 tctttataac cgtcaactat agcttgaaaa ccggttcctc cccgctccat aagattggca
      241 acatccaaac aagcggatat gattgtgttt ctacgaacgg agggaataga acctatagga
      301 tattcttcat aactttttgg taacaaccac gaaccgggcg aaacaatatc aattctgtca
      361 ctgtaaatat ctacatcaat ctgcgttccg taaatagagt aatccctgtg tgcgattgca
      421 tttaccaatg cttcacatat agctttttta ggataggaac gaatctcttc acgaccgccg
      481 ttttccgttt tgtgccaacc tgtttttgtg tttctctcaa taaacctgag tgtttcatta
      541 aaaacatcgg gcaacggtcc tttgaatctg ccgttatcca atacttctcc gacttttgtt
      601 ttacctctcc ataaacgaca gcagattaat gtatcatcac cgtcatatcc gtctctaaac
      661 atcagaaaac ctgtttttgc gtaaccgtct ttggaaacaa tctcatagtt ttgcaactct
      721 tttacagtgg gtaccgaacc atcctttctg aactccctgc ataattcggc ataacttgtc
      781 cattgctttt ccgcataaag agtttcgctt atctcattat ccacaccgaa tttccttttt
      841 gataaggtga ttatttcttc cggcgttgcg ggagcggaat agccgttacc ttttatgtat
      901 acggtttcat taaaatcacc ctcacgatac cttacaatgg attccgaagg agaaatgttt
      961 attgcaagaa caaatttctc cgcagcatta tcaacatttc gcatcagata gcgcaactta
     1021 acatgaggaa aaatatgtcg gtcatttatt tttgcaacaa ggttttttgt tttatcaatt
     1081 tcatccaagc tgataccgaa ggcttcccgg tcttcggaaa ctcccacaaa cagcataccg
     1141 ccgttaccgt tagcaaaacc cacaatgctt ttagcccact tcacaggatt atccgaatta
     1201 agtaccgctt tgaattcaca ctccatatct tccgttatta catctgagaa taattcggtt
     1261 atcttcataa aacgctctcc tttttcattg ctccgctaaa ttatatcaaa cattgcccga
     1321 cattgcaata acatttctgt acattacaac aacattgccc ggcattacaa tcacattgcc
     1381 gaccatattg tgacattact cataaatcct ctgactttta atctgccgat ttttgatggc
     1441 ttcttgaatc aaaagataat acttataaaa atacatctca ctgctacgga cagcatcaat
     1501 tacatagtgc ataacaaaac ataattgtgt ataattttat tgatattaaa gtgttgaccg
     1561 gaaagccctt tttccggaac ctatattact acagtagagg ttaaagaatg aaaaaaatct
     1621 atacaggaaa aactaaagac gtatactgcc tggaagacgg aaattatctt ctgaagttca
     1681 aggatgatgt gacgggaacc gacggggttt tcgatccggg agccaatacc gtcggactga
     1741 aaatggaagg ctcgggaaaa gcgggactga aacttacaaa gttcttcttt gaaaaactga
     1801 acagtctcgg cattcccacc cactatatat ccgcagatat agacaacaat acaatgaccg
     1861 ttaaacccgc aaaaatgttc ggaaaaggtg tggaagtcat attgcgatac agggctgtgg
     1921 gcagtttcta cagacgatac ggaatgtatt gtaccgaagg acagcattta gacggttatg
     1981 tggaagtgac attgaaagat gacgacagaa atgaccctct tataaccgat gaagcgttgc
     2041 agatgctgaa gataatgaca aaagaggaat atcgtattct tgtggagttg acaagaaaaa
     2101 caggttctat agtaaaagag gaaatgctca aaaaaggatt ggagctttat gacataaagc
     2161 tggagttcgg aagagtgggt acggacaatc atatagcact tatagatgag atatccggcg
     2221 gtaatatgag ggcatattgc aaaggcaaat atgtcgaacc gttggaattg gaaaagataa
     2281 tgctgaaata gataggaata tatgcattta caaaacagga ggtatatatg aaaaagcgtg
     2341 ttaaacgtgt taaaaaagaa aaagtaaaac tatttgagga gttcaagaag tttataacaa
     2401 ggggaaacgt aatagatctt gcggtcggta tcctgatagg atcggcattt accaaaatag
     2461 tttcttccct tgcaaatgac atcattgttc ctttagtggg ccttataatg ggaaaaataa
     2521 atctaaagga gcttaaatgg gtactgacat atgatgcgga aggcaaagca ctctcttcgg
     2581 tcaattacgg catgtttata cagtacattg tggattttct gataatggcc gtatgtgttt
     2641 tcatgttggt aagactcttt acggctctta ggaaaaagag cgaagaactt aaagagaaag
     2701 caaagaaagc ggaagaagct gcagctgcag ctacgccgcc cccgccaccg gcaccgcccg
     2761 aaccgacaaa agagcaactg cttcttacgg aaatacgcga tatactgaag gcacagaaat
     2821 gagtgagaac aatttacaaa aacccaatat taccgtaacg gaatacaatt ccgatgaaat
     2881 tcgagaatat tcgacagatt ctttggaaac acttaaaaag gatgtaaaag gggttcgctg
     2941 gattgatatc gacaatgaat gcggcaaggt attatgcgag cagatgtcaa gactgtataa
     3001 tatccatccc gtagttttgg aaagtatata tgaaaaagac caaagtccca aactggatga
     3061 ttatcaggac tttctgtttg ccgttgccac tatgatttac aaaaaagaac aggcccttgt
     3121 ggcagagggt atttcctttt tgttaggcga caattacgtt atttccatag gaaatctgga
     3181 cggggatgtt tttgataaag tgcgaaaaga gttacatacc cccaaaagcc gcatacgcat
     3241 gtccggtccc gattatctgc tataccgtct tatggactcc ataaccgata attactttgc
     3301 caccctttac gagattgagg aacggatata ccggttggaa gaggaaataa tcaaagatcc
     3361 gtccgactca ctgttggcaa aaataaggga ggtaaagagc gataaccaac tggttgcaaa
     3421 atccatcata ccttttaaag agtcccgctc aattttgatt aacatgcctt caaaattcat
     3481 tacctcttct gtcgcccctt atatgaatga cgtttacgat cacataaaag aagctatagg
     3541 attgtcggaa acctgtcgtg taatgataaa cgatctgatg cagctgttct atgcctccac
     3601 aagctataaa cttaacgaga taataaaagt acttaccgtt atatccacaa tattcattcc
     3661 tctgactttc attgtgggat tgtacggtat gaacttcaag cacataccgg aatttaacat
     3721 accctgggct tatcccgtgc tattgggagt tatgctggtt ataaccttgt ttatgatata
     3781 ttatttcaaa aaaaagaaat ggttctgata aaaaagctat tccgatatat aacaaa
//
LOCUS       DAOPWC010000101         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24103_length_3835_cov_3.168783, whole genome shotgun sequence.
ACCESSION   DAOPWC010000101 DAOPWC010000000
VERSION     DAOPWC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24103_length_3835_cov_3.168783"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKJ65_03115"
     CDS             <1..279
                     /locus_tag="PKJ65_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40862.1"
                     /translation="FLWRMCSIYHDLLDRELAMDFVFASVRDGASGVFSCITELKEHN
                     KNVVEGFIQACEIVEDMLKKGAGRADVGAMMSSRGKDIFWEKWRTQYI"
     gene            383..1243
                     /locus_tag="PKJ65_03120"
     CDS             383..1243
                     /locus_tag="PKJ65_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNZ40863.1"
                     /translation="MRLRRIITSILIIMILLSPLITINVDASAEISPQGIKYWVTNSP
                     VIYDYKNIAFKNDKLIVDGYLIVPVTEFFDFFGRYEYEWDNDKKSVTVTDGNNTYQIY
                     SGTRYLLENGHKIQAPAMNFIYNGKIYASLKVMADALGLKTLYEEVSDSILLTKHSFY
                     FNAYYTYEDLYWLSRIVHAEAAHESYEGKVAVANVVLNRVRHDSFPDTIYGVIFDKAG
                     GVQFTPVAAGTVYNDPSEEAIRAAKDALNGYNNVSWCLFFFNPRLAKSFWIADTCDFY
                     KSIGNHDFYF"
     gene            complement(1245..2714)
                     /gene="glpK"
                     /locus_tag="PKJ65_03125"
     CDS             complement(1245..2714)
                     /gene="glpK"
                     /locus_tag="PKJ65_03125"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNZ40864.1"
                     /translation="MKKYVIALDQGTTGSRAVAFDHDGKCVGSVNKEFTQFFPSPGWV
                     EHDPYDILQSQMGALFELIKKLAVKPEQIAAIGITNQRETVVVWDKMTGKPVYNAIVW
                     QCRRTAGICEQLKADGYADLIKSETGLVLDAYFSGTKIKWILENVKEVKNKSLLCGTI
                     DTWLIYNLTKEKSHVTDMSNASRTMLFNINTLEWDKQLLDILGIKKEMLPKVVPSSCV
                     VGHMELEGISIPIAGIAGDQQAALFGQACFEPGEVKNTYGTGCFILMNTADKPVFSDK
                     GLITTIAWNLNNKTYYALEGSIFNSGSAVQWLRDQLGLIKTAAETERMAFAVEDTDDV
                     YFVPAFTGLGTPHWDMYARGVLVGITRGTNKNHIARAVLESMAYQTVDVIDCMQQCSD
                     VALKCLKVDGGASANNFLMQFQADMLNCDVVRPVMQETTSLGAAFLAGLAVGFWKDVE
                     ELKGIIKQDRIFVPKDFFDREYRKKRWHEAIKRSKNWAR"
     gene            complement(2731..>3835)
                     /gene="murD"
                     /locus_tag="PKJ65_03130"
     CDS             complement(2731..>3835)
                     /gene="murD"
                     /locus_tag="PKJ65_03130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNZ40865.1"
                     /translation="IKSGATLTSELCEFIKYCNAKIIAVTGSSGKTTTTTLIGELLKQ
                     QGYKVWVGGNIGTPLFHRLDDISVNDYVVLELASCQLQMFGNKSPNISVITNITPNHL
                     DFHDDYDEYIYCKSIIFKHQKSDDIVILNQDNDITRQLRESVQSKAYMFSMNEKYDKE
                     NSVNGAYLHDNAIYVNIDKNIKRMLDVNDIKIPGRHNIENYMAAILATYSLVEQAAVK
                     HVATTFNGVEHRVEYVRTVNGVRFYNDSIGTTPSRTVASLHCFDKKVILIAGGYDKNI
                     SYDIMGPLINEKVKGLVLMGQTKEKIKQAYTDYSCTATLCEASTMEEAVRKAFNMAEE
                     GDIIILSPASAAFDMFKNFEVKGNIYKQIVNSL"
BASE COUNT         1030 a          853 c          747 g         1205 t
ORIGIN      
        1 ttcctttgga gaatgtgttc aatatatcac gatttactgg accgtgagct ggcaatggac
       61 tttgtattcg cttcggtgcg tgacggagcc agcggtgtat tctcctgtat caccgaactg
      121 aaagaacata acaaaaatgt tgttgagggt ttcattcagg catgtgaaat tgttgaagat
      181 atgcttaaaa aaggagcagg cagagccgat gtaggcgcaa tgatgtcttc cagaggtaaa
      241 gacatattct gggaaaaatg gcgtactcag tatatataat ggaactgatt ttattgcctt
      301 tgttcacaat ttgttcacaa tttgcggttt tgctattgca ttaccgtatt ttttggtgta
      361 atataccgaa atgatgaaag atttgagact aagaagaata ataacttcga tactgataat
      421 aatgattctg ctctcaccgt taataacaat caatgttgac gcaagtgccg agatatctcc
      481 tcagggtatt aaatattggg ttaccaactc ccctgtgata tatgattaca agaatattgc
      541 gtttaaaaat gacaaactga tagtagacgg ttatctgatt gttccggtga cggaattctt
      601 tgattttttc ggcagatatg aatatgaatg ggataatgat aagaaaagcg ttacggtaac
      661 cgacggtaat aatacctatc agatttattc gggaacgcgc tatcttctgg aaaacggcca
      721 caagatacag gctcctgcca tgaactttat atacaacggc aagatatatg cttcactgaa
      781 agttatggcc gatgcattag gtttgaaaac cctatatgaa gaagtttccg attccatatt
      841 gcttaccaag cattctttct atttcaacgc atattacact tatgaggatt tatattggtt
      901 gtccagaata gtgcatgcgg aagcggcaca tgaatcatat gaagggaaag tggcggtagc
      961 aaatgtggta ctcaatcgtg taagacatga cagttttccc gacacgatat acggcgttat
     1021 attcgataag gccggaggtg ttcagtttac accggtagcc gcaggtaccg tatacaatga
     1081 cccttcggaa gaagcaatcc gtgcggcaaa agacgcattg aacggttata acaatgtgtc
     1141 ctggtgttta ttcttcttta atcccagatt ggcaaagagt ttctggattg ccgatacctg
     1201 cgacttttac aaaagcatag gtaatcacga tttctatttt taaactatct ggcccagttt
     1261 tttgaccttt tgatggcttc atgccatctt ttttttctgt attccctgtc aaagaagtct
     1321 ttgggcacga atattctgtc ctgctttatt atacctttaa gctcttcaac atccttccag
     1381 aagcctacgg caagtcctgc cagaaaagcc gctcccaaag aggtggtctc ctgcataacc
     1441 ggtctgacta catcgcagtt aagcatgtcg gcctggaact gcattaaaaa gttgtttgca
     1501 cttgctccgc cgtctacttt caggcacttt aaggcaacat ccgagcactg ctgcatacag
     1561 tctatgacat ccaccgtctg atatgccatg gattccagaa ccgctcttgc tatatgattt
     1621 ttgtttgtgc ctcttgtaat acccacaagc actcctcttg catacatatc ccaatgggga
     1681 gtacccagac cggtaaacgc cggaacgaaa tagacatcat cggtatcttc caccgcaaaa
     1741 gccattctct cggtttccgc tgcggttttt ataaggccca gctggtcgcg caaccattga
     1801 acggcggaac cgctgttgaa tatgctgcct tccagtgcat aatatgtctt attattcaga
     1861 ttccatgcaa ttgtggttat cagcccttta tcggaaaata ccggcttatc cgcggtgttc
     1921 atcagtatga agcatcccgt tccgtaagta ttcttgactt caccgggctc gaaacatgcc
     1981 tgtccgaaca gtgccgcctg ctgatccccc gctatccccg caatgggaat acttattcct
     2041 tccagttcca tatgacctac aacacaggaa gaaggtacca ctttcggaag catttctttt
     2101 ttaataccta atatatctaa aagttgttta tcccattcca acgtattgat gttaaacaac
     2161 atggttctgg aagcattgga catgtcggtg acatggcttt tttccttggt aaggttgtat
     2221 attagccagg tatctatggt accgcaaaga agactcttgt tttttacttc cttgacattt
     2281 tccagaatcc atttaatctt ggttccggaa aaatatgcat ccagtaccag accggtctcg
     2341 gattttatca aatcggcata tccgtcggct ttaagctgtt cgcatatacc cgccgttctt
     2401 ctgcactgcc aaacaattgc gttataaacg ggttttccag tcattttatc ccataccaca
     2461 accgtttccc gctgattggt aatacctata gcggcaatct gttcgggctt gacggctaat
     2521 ttttttataa gctcgaaaag cgcacccatc tgactttgca aaatatcata cgggtcatgc
     2581 tccacccacc cgggagacgg aaagaactga gtaaactctt tgttgacaga accgacacat
     2641 ttaccgtcat ggtcgaaagc gacagctctg ctgccggttg ttccctgatc caatgcaatt
     2701 acatattttt tcatgacaca cctgctttat ttatagtgaa tttacaatct gcttatatat
     2761 attaccttta acttcaaagt tcttaaacat gtcgaaagct gcacttgcgg gagacagtat
     2821 aattatatca ccttcttctg ccatgttaaa tgccttgcgg acagcttctt ccattgtaga
     2881 cgcttcacac agtgttgcgg tgcaggaata atcagtgtat gcctgtttta tcttttcctt
     2941 ggtttgcccc ataagaacca gccccttaac cttttcgttg atgagcggcc ccattatgtc
     3001 gtaagatatg tttttatcgt aacctccggc aatcagtatt actttcttat caaaacaatg
     3061 cagacttgcc accgttctcg aaggtgtagt acctatggag tcattgtaga aacgcactcc
     3121 gttaaccgtc ctgacatatt ccaccctgtg ctccacaccg ttaaaggtag ttgcaacatg
     3181 ctttactgcg gcttgctcca caagcgaata tgtcgcaaga atggccgcca tatagttttc
     3241 tatattatgt cttcccggaa tctttatatc attaacatcc agcatcctct ttatgttttt
     3301 gtcaatattg acatatatgg cattatcatg aaggtatgca ccgtttaccg aattctcttt
     3361 gtcatacttt tcattcatgg aaaacatata tgcttttgac tgcaccgatt ctctcaattg
     3421 tcttgttata tcgttatcct gatttaaaat tacgatatca tcacttttct gatgcttaaa
     3481 gattatactt ttacagtaaa tatattcatc gtaatcgtca tgaaaatcca gatggttggg
     3541 tgttatgttt gtaataacgg aaatattcgg actcttgttg ccgaacatct gcaactggca
     3601 ggatgccaat tccagtacga cataatcatt cacacttata tcatccagtc tgtggaataa
     3661 cggtgtgccg atattacctc ccacccatac tttatatccc tgctgtttca gcaattcccc
     3721 tattaaagtg gtggtagtgg tcttaccgga acttccggta acggcaatta tcttggcatt
     3781 acagtatttt ataaactcac acaattcgga tgtaagagtt gccccgctct ttatt
//
LOCUS       DAOPWC010000102         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24110_length_3834_cov_3.185499, whole genome shotgun sequence.
ACCESSION   DAOPWC010000102 DAOPWC010000000
VERSION     DAOPWC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24110_length_3834_cov_3.185499"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKJ65_03135"
     CDS             <1..385
                     /locus_tag="PKJ65_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ40866.1"
                     /translation="KCKVRSIEFSLMQRCAAHIGSRTDIEEAFLAGQQAVKAAVEGHT
                     DYMIGFERTNVKGKYGIKTVLIPLAKVANAEKTVPLEWFNQEKNFLKKDFIDYALPLI
                     QGDSGLPFEEGLPKFAKLKKVSARK"
     gene            399..1337
                     /gene="fba"
                     /locus_tag="PKJ65_03140"
     CDS             399..1337
                     /gene="fba"
                     /locus_tag="PKJ65_03140"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNZ40867.1"
                     /translation="MALITSKKMFEKAYKGGYAIGAFNVNNMEIIQGITEAAKEVQAP
                     LILQVSAGARKYAKHVYLMKLIEAALEDTGLPICVHLDHGDSFETCKSCIDGGFTSVM
                     IDGSHLPFEENIRLTKQVVEYAHSKGVVVEGELGRLAGVEDDIKVKKEDSSYTDPDEV
                     EEFVSKTGVDSLAIAIGTSHGAYKFQGEAKLRFDILEEVQKRLPGFPIVLHGASSVIP
                     EFVEKINKYGGKMPGAQGVPEEMLRKAAGMAVCKINIDSDLRLAMTSTIREYFALYPD
                     HFDPRQYLKPARENIKQLVKHKLVHVLGCDNTAREY"
     gene            1451..2068
                     /gene="yihA"
                     /locus_tag="PKJ65_03145"
     CDS             1451..2068
                     /gene="yihA"
                     /locus_tag="PKJ65_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNZ40868.1"
                     /translation="MLVKDSKFIISAAKAEQWPDDEIPEVVFSGKSNVGKSGLINALC
                     NRKNLAYSASAPGKTRLINFYEINGVMRMVDLPGYGYSKASKRETKLYGKLAERYLTE
                     RKNIRLIIQILDIRHDPTEDDLHMIEFLNTIGLPFVIAVNKCDKLSKSQAVNRARNIA
                     GMLDIDPDTPFILTSVTAKRGMEEIWEAIDFYCFDQTDDDNNETE"
     gene            2083..3147
                     /gene="tig"
                     /locus_tag="PKJ65_03150"
     CDS             2083..3147
                     /gene="tig"
                     /locus_tag="PKJ65_03150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ40869.1"
                     /translation="MKNRKINHTKEIRILSLITVTVLLLTMLLTGCAPYKNSRYIELA
                     EYMNLQLDYNLIAVTEEDLQKETDILLDKYAKTVVLDKGEVKSGDIVNIDYSGLVDEK
                     PFAGGAETGYDLTIGSHTFIEGFEEGLIGAVIGQTVVLNLTFPVNYKDKDNSVSALAG
                     KEVVFNVTVNKVTRRVPPEYTDEFIKSLDNEYKTVEEYNEYLKKEIFKYKKTQEAWKT
                     VVNNTVVIKYPDTVQKRVDEIREYYEYYTIYYGYSSFNDFCIDALGQTEEEFNRINLE
                     SARDGVKQEMISQAIAGLEKIKLTKSDLDQGYANYAEYYGYDNTQDLFKDYDKNSITS
                     SILLNKVMEYVAKHAVVVKD"
     gene            complement(3175..>3834)
                     /gene="ilvE"
                     /locus_tag="PKJ65_03155"
     CDS             complement(3175..>3834)
                     /gene="ilvE"
                     /locus_tag="PKJ65_03155"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNZ40870.1"
                     /translation="RPFIISVDPFLGVHPSYTYLFMIIIGPVGPYYPGGLNPTSIYVE
                     DFYKRASKGGMGHAKTMGNYAASLIAQEKAIKLNCIQTLWLDAHEGKYIEEVGAMNVF
                     FVIGDKVITPELSGSILPGITRKSTLEIAEALGHKTEERKISVDEVIKALKNGDMKEC
                     FGTGTAAVISPVGELVYKDVRYVINDNKIGKISQKLYDTLTGIQYGIIKDTRGWTLEV
                     K"
BASE COUNT         1247 a          691 c          879 g         1017 t
ORIGIN      
        1 caaatgtaaa gttcgttcca tagagttcag ccttatgcag cgttgtgcgg cgcatatagg
       61 ctcaagaacc gatatagaag aagcttttct tgcaggacag caggcggtaa aagctgcggt
      121 agagggacat accgattaca tgataggttt tgaaagaaca aacgtaaaag gtaaatacgg
      181 tataaagacg gttcttatac ctcttgctaa agtggccaat gccgagaaaa ccgttccttt
      241 ggagtggttt aaccaagaga agaatttctt aaagaaagat ttcatcgatt atgcacttcc
      301 tttgatacag ggagattccg gtcttccttt tgaagaagga ctgcctaaat ttgcaaaatt
      361 gaaaaaagta tcagcaagaa agtagagagg atatagatat ggcacttata acatcaaaga
      421 aaatgtttga aaaagcctat aaaggcggat atgcgatagg cgcattcaat gtaaacaata
      481 tggagattat ccaaggtata acggaagccg caaaggaagt tcaggcaccc ctgatacttc
      541 aggtttcggc aggcgcaagg aaatatgcaa agcatgttta tcttatgaaa ttaattgaag
      601 cggctcttga agataccggg cttcccatat gtgttcacct ggaccacggc gacagttttg
      661 aaacgtgtaa atcctgtata gacggaggtt ttacttcggt aatgatagac ggttctcatc
      721 ttccctttga agaaaacatc agattaacaa agcaggttgt ggaatatgca cactcaaaag
      781 gtgtagtagt cgaaggagaa ttgggcagac ttgcgggagt ggaggatgac attaaggtca
      841 aaaaagaaga ttcttcctat accgatcccg atgaagtgga agagtttgta tcaaaaaccg
      901 gagtggactc actggcaata gcaataggta caagccacgg agcatacaag ttccagggtg
      961 aagccaagct gagatttgat atattggagg aagtgcagaa aagattgccc ggtttcccga
     1021 tagttcttca cggtgcatca tcggttattc cggagtttgt ggaaaagata aacaaatacg
     1081 gcggtaaaat gcccggcgct cagggagttc ccgaagaaat gctgagaaag gctgcaggta
     1141 tggcggtatg taagataaat atagactccg atctgaggct tgcaatgact tcaaccataa
     1201 gagagtattt tgcattgtat cccgatcact tcgatccgag acagtatctg aaacctgcaa
     1261 gagaaaacat aaagcagttg gttaaacata aacttgttca tgttttggga tgtgacaata
     1321 cggcgagaga gtattaaaat aagcgggaaa ttaaacataa aaaagcggga aaccgctttt
     1381 tttatggaaa aaacttaaaa gttcgcaata tcgggaatgt aatgtataat attggcaaag
     1441 gagtaccgat ttgttagtaa aagattccaa gtttataata agtgcggcaa aagcggaaca
     1501 atggcctgat gatgagattc cggaagttgt gttttccgga aaatccaatg tgggtaaatc
     1561 cggccttata aacgctcttt gcaacagaaa gaatcttgcc tactcggcat cggctccggg
     1621 taaaacccgt cttattaact tctatgaaat aaacggtgtc atgagaatgg tggaccttcc
     1681 cggttacgga tactccaaag cttcaaagcg ggaaacaaag ttgtacggta aacttgccga
     1741 acggtattta accgaaagaa agaatatacg gctcataatt cagatattgg acattcgcca
     1801 tgaccccacg gaagatgatt tgcatatgat agagtttttg aatactatag gtttgccgtt
     1861 tgttatagct gtaaataagt gcgataaact ctccaaatct caagctgtga acagagcaag
     1921 aaacatagcg ggaatgctgg atattgaccc cgatacgccg ttcatattga cttccgtaac
     1981 ggcaaaaaga ggtatggaag agatatggga agctatagat ttttattgct ttgaccaaac
     2041 ggatgacgat aataacgaaa cagagtaaac taaaggacgg atatgaagaa caggaagatt
     2101 aatcacacta aagaaataag gatattatca cttattacgg taacagtgct gttacttaca
     2161 atgctgctta ccggatgtgc tccttataag aacagcaggt atatagaact tgccgaatat
     2221 atgaatcttc agctggacta taacctgatt gccgtaaccg aagaagattt gcaaaaagag
     2281 acagatattc ttttggacaa gtatgcaaaa accgttgttc tggataaggg agaggttaag
     2341 tcaggcgata ttgtaaatat cgattattcc ggtctggtag acgaaaagcc ttttgcgggc
     2401 ggtgccgaaa caggttatga tctgaccata ggatcacaca cttttataga gggatttgaa
     2461 gaaggactga taggggcagt tatcggacag acggttgtgt tgaatcttac atttcccgta
     2521 aattacaaag ataaggacaa ctctgtgtcc gcacttgccg gcaaagaggt agttttcaat
     2581 gttacagtga ataaagtcac aaggcgagta ccgccggagt ataccgatga gtttataaaa
     2641 agcctggata atgagtataa gacggtggaa gaatacaacg aatatctgaa aaaagagata
     2701 ttcaagtata agaaaactca agaagcatgg aaaaccgtgg tgaataatac agtagtaata
     2761 aaatatcccg ataccgttca aaagagagtg gatgagataa gagaatatta tgaatattac
     2821 acgatatatt acgggtacag ctccttcaac gatttctgta ttgacgcatt ggggcagacc
     2881 gaagaagagt ttaaccggat taatctggaa agcgcccgtg acggagtgaa gcaggaaatg
     2941 atatcgcaag ccattgcggg gttggagaaa ataaagttaa ccaaaagcga tttggatcaa
     3001 ggctatgcca attatgccga gtattacgga tatgacaaca ctcaggattt gtttaaagat
     3061 tacgataaaa attccattac ctccagcata ctgttaaata aggtaatgga atatgttgca
     3121 aagcatgcgg ttgtggtaaa agattaggta tcttaaaaag tattatttaa tatattactt
     3181 aacttctaaa gtccaacccc tcgtatcctt aatgataccg tactgtatgc ccgtaagcgt
     3241 atcatagagc ttttgggata tcttgcctat cttattgtca ttaataacgt aacgcacatc
     3301 cttgtataca agttctccta cgggggatat taccgctgcc gttcccgttc cgaaacattc
     3361 tttcatgtca ccgtttttca gcgcttttat aacctcatca acggatattt ttctttcctc
     3421 cgtcttgtgg cccaatgctt ctgcaatctc caatgtgctt tttctggtta ttccgggtaa
     3481 tatgcttccc gaaagttccg gtgtaattac tttatcgccg atgacgaaga atacgttcat
     3541 tgcgcctact tcttcaatat acttaccttc atgcgcatcc agccacaaag tctgaataca
     3601 attgagtttt attgcctttt cctgtgcgat aaggcttgcc gcgtaattac ccatggtttt
     3661 ggcatgtccc attccgcctt tggaggcacg tttatagaag tcttccacat aaatactggt
     3721 gggattgagt ccgccgggat aatacggacc taccggccct attattatca taaaaagata
     3781 tgtataggaa ggatgcactc ccaggaaggg atcgacagag ataatgaacg gacg
//
LOCUS       DAOPWC010000103         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24184_length_3823_cov_3.721868, whole genome shotgun sequence.
ACCESSION   DAOPWC010000103 DAOPWC010000000
VERSION     DAOPWC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24184_length_3823_cov_3.721868"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PKJ65_03160"
     CDS             complement(<1..1164)
                     /locus_tag="PKJ65_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ40871.1"
                     /translation="MTTLKMTVNTTVKKGHISPLIYGSFAEHLGRCIYDGIYVGDNSD
                     IPDTDGIRNDIVEALKAIKLPVLRWPGGCFADEYHWRDGIGDKSKRKKMINTHWGMVI
                     DDNSFGTHEFLRLCELIGCEPYIAGNVGSGTVREMAEWVEYLNCAAPTSVVLERTANG
                     RIEPWKVKYFGIGNENWACGGNMRPEYYADLYRRYAVYCRNYGSGRLYKIACGPSSHD
                     YNWTDKLMESITATKDCSAISLHHYCLMTNVRATELSSTDYAKTISKAQAMDKIIEKH
                     WEIMGKYDPDRRIKLVVDEWGTWYRSEPGTHPSFLHQQNTMTDAIVAAGTLDIFNNKC
                     NIVDMANIAQTVNVLQSVILTSGNQLIKTPTYHVFDIYKEHQNNMLLESSCENR"
     gene            1308..2354
                     /locus_tag="PKJ65_03165"
     CDS             1308..2354
                     /locus_tag="PKJ65_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40872.1"
                     /translation="MEFTPDYRNIRDAAYNKKAKRLPMYEHSISTDHMEKILNKKFAA
                     LIDGDFSDKCEFFNRYCLFFKTMGYDTVSFEVAIRESFPGGGALDKHITPAITSREDF
                     INYPWEEIPELFLKRADEFYMALKQSLPPGMKMIGGPGYGIFECVQDLTGYMNLCIMS
                     YEDPELYADIFLAMRKLFVKIWQGFLDKYADMCAVARMGDDLGYKSNTMLSAEDIKRY
                     IVPGYADIVRLSHSYGLPFLLHSCGNIFNVMEDIINVAKINAKHSNEDQIAPFSVWVE
                     RYGDRIGNFGGVDADVLCRTDKQQLKEYITNLLYSVGNEKGIAIGTGNSVPDYVPTEN
                     YINMIETVREYRKG"
     gene            2365..3519
                     /locus_tag="PKJ65_03170"
     CDS             2365..3519
                     /locus_tag="PKJ65_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40873.1"
                     /translation="MRDFEQLKKMTADFYSMKPDAPIPLEEFGYYSKEQWISQGYIKE
                     GEDMVALFGFERLTNYYLHGLAGTVAPFVPRFKEEVIHSDEQYEIAIDKAGRTVKYFK
                     GKREGFMPQYLDFPVKDMATWEEIKWRLDYANAQRNEDLKKFISDSVPLAEKGYHSVM
                     YIVGAFMYLRSLVGGEEILYMIYDNPGLLHTCMEKWLELSDYVTAKYQDHVRIDELLL
                     DEDICYNHGPLISPEQIRTFLFPYYKELIQGLRSRNARLAMSDKECAYKQPKAYDLII
                     HVATDGDCRPVIPLYKEIGMNYMSPMEVASGCDVVQIRKEHPDIRIKGGIDKRILAHS
                     KEAIKAEIDRILPFMKKQGGYLPTCDHGVPPEVPFENYVYFRELLKDYAR"
     gene            3683..>3823
                     /locus_tag="PKJ65_03175"
     CDS             3683..>3823
                     /locus_tag="PKJ65_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ40874.1"
                     /translation="MYYDICIIGAGIVGSLIARQLSKYKVKIAVIERHVDVSMDATGA
                     NSG"
BASE COUNT         1083 a          758 c          878 g         1104 t
ORIGIN      
        1 tctgttctca cagctgcttt ccagaagcat attgttctgg tgttccttgt atatatcgaa
       61 cacatggtaa gtgggtgttt taataagctg gttgccgctt gtcagaatta ccgactgcag
      121 aacatttacc gtctgggcaa tattggccat gtctacaata ttacacttgt tgttgaatat
      181 atccagcgtt ccggctgcta ctatggcatc ggtcatggtg ttttgctgat gaaggaagct
      241 cggatgggtt cccggttcgg acctgtacca ggtgccccat tcatccacaa ccagcttgat
      301 tctgcggtcg ggatcgtatt tgcccattat ttcccagtgt ttctctatta ttttgtccat
      361 tgcttgtgct ttggaaattg tttttgcata atcggttgac gataactcgg tcgctcttac
      421 attggtcatt aagcaatagt gatgcagaga aatagccgaa cagtctttgg tggcggttat
      481 gctttccatt agcttgtcgg tccagttata atcgtgagaa ctgggtccgc atgctatttt
      541 atataacctg ccggaaccgt agtttctgca gtagaccgca tatcgtctgt aaagatccgc
      601 atagtactcg ggtctcatat tgcctccgca tgcccagttt tcatttccta ttccgaagta
      661 ttttactttc cacggctcaa tgcgtccgtt tgccgttctt tccagcacaa ccgatgtagg
      721 tgcggcacag tttaagtatt ccacccattc ggccatttca cgaacggtac cgctgcctac
      781 attgccggca atatacggct cacaccctat aagttcgcac aatcttaaga attcatgagt
      841 accgaaactg ttatcatcta tgaccatacc ccaatgggta ttaatcatct tctttctttt
      901 tgacttatct cctataccgt ctctccagtg atattcatcg gcaaagcatc ctcccggcca
      961 tctcaatacg ggcagtttaa ttgccttcag cgcttccact atatcattcc ttataccgtc
     1021 ggtatcgggt atatcggaat tgtctcctac gtatatgccg tcatatatgc atcttcccag
     1081 gtgctcggca aagcttccgt atataagggg acttatgtgt ccttttttaa cggttgtgtt
     1141 tacggtcatt ttcagtgttg tcatgatgta cctcttgaat ttatagtttc atctcattat
     1201 accatgttaa tgaatgtact cttaaaatat atgtataggt gatagttata tttttaaggt
     1261 gtatttgatg atataattag aaaaaagata agtattaaag gacggttatg gaattcacac
     1321 cggattacag aaatatacgg gatgcggcat ataataaaaa ggcgaaaagg cttccgatgt
     1381 atgaacacag tatcagtacc gaccacatgg aaaagatact gaataaaaag tttgcagctc
     1441 ttattgacgg cgatttttcc gataagtgcg agttctttaa tcggtattgc cttttcttta
     1501 aaaccatggg atatgataca gtttcttttg aagtggcgat aagagagtct tttccgggcg
     1561 gcggagctct ggataaacat attactcccg ccataacatc aagagaggat tttatcaatt
     1621 atccgtggga ggaaataccg gagttatttc ttaaaagggc ggatgaattc tacatggctt
     1681 tgaaacaatc gctccctccg ggcatgaaaa tgataggcgg tcccggttac ggtatattcg
     1741 aatgtgttca ggacctgacg ggatacatga atctttgcat aatgtcctat gaggaccccg
     1801 aactgtatgc cgatattttt ttggcaatga gaaaactgtt tgttaagata tggcaagggt
     1861 tcctggataa atatgccgat atgtgtgccg tagcaagaat gggcgacgat ttggggtata
     1921 aatccaacac catgctttca gccgaagata taaagaggta tattgttccg ggctatgccg
     1981 atattgtacg actttcccac agctacggct tgccgtttct tttacattcc tgcggtaata
     2041 ttttcaatgt aatggaggat attatcaatg tggcaaagat aaatgccaag cattccaatg
     2101 aagaccagat agcaccgttt tccgtgtggg tggagcggta cggagaccgt ataggtaatt
     2161 tcggaggagt ggatgccgat gtattgtgca gaacggataa acagcagttg aaggaatata
     2221 ttaccaatct gctttactcg gtgggtaacg aaaaaggtat agcaataggt accggcaatt
     2281 ccgttcccga ttatgtgcct accgaaaact atataaatat gattgaaacg gtaagagaat
     2341 acaggaaagg ttgatttata cagtatgaga gattttgagc aacttaaaaa aatgaccgca
     2401 gatttctatt ccatgaaacc cgatgctccc ataccgttgg aagagttcgg ttattacagc
     2461 aaagagcagt ggataagcca aggatatatt aaagaaggtg aagacatggt agcactcttc
     2521 ggttttgaac gacttacaaa ctattacctt cacggtcttg ccggaacggt agccccgttt
     2581 gttccccgat tcaaagaaga ggttattcat tccgatgaac aatatgaaat agcaatagac
     2641 aaagccggaa ggactgtcaa atacttcaaa gggaaaagag aaggttttat gccccaatat
     2701 ctggattttc ccgttaagga tatggccaca tgggaggaaa tcaagtggag actggattat
     2761 gcaaatgccc aaagaaacga ggacctgaaa aagtttatat ccgactccgt accgttggca
     2821 gagaaagggt atcattcggt catgtatatt gtgggtgctt ttatgtatct gcgttcgctc
     2881 gttggcggtg aagaaatact gtatatgatt tatgacaatc ccggactgct tcatacctgc
     2941 atggaaaagt ggctggaatt atccgattat gtaacggcaa aatatcagga ccatgtaaga
     3001 atagatgaac tgctgctgga tgaagacata tgctacaatc acggcccgtt aatatctccc
     3061 gagcagatac gcacctttct gtttccctat tacaaagaac ttatacaggg attaaggagc
     3121 agaaatgcgc gacttgcaat gagtgacaag gagtgtgcgt acaaacaacc caaagcctat
     3181 gaccttataa ttcatgttgc caccgacggt gactgcagac cggtcattcc gttgtataaa
     3241 gagataggta tgaactatat gtctcccatg gaagtggcct cgggatgcga tgtggtgcag
     3301 ataagaaaag agcatcccga catacgtata aaaggcggta tagataagag aatactggct
     3361 cacagcaaag aggccataaa agcggaaatt gacaggatct taccttttat gaaaaaacag
     3421 ggaggctatc tgcccacctg cgaccacggg gttccgccgg aagttccttt tgagaactat
     3481 gtgtatttca gggagttgct taaagattat gcaaggtagc gtatcattgc aaaaaagacg
     3541 gctcgggtat aaaattaatc agtaaggaag ggagatcaca tgaagaaagc aatagcaatc
     3601 atactggccg caatactggt gctttccctg attctgacaa tgattttaca gagcggcgta
     3661 ttgtatagat aacggaggat gtatgtatta cgatatatgt attatcgggg ccggtatagt
     3721 cggcagcctc atcgcaagac aattgtccaa gtataaagtt aagattgcgg taatagagcg
     3781 acatgtcgat gtatcgatgg atgctacggg tgccaattcc ggt
//
LOCUS       DAOPWC010000104         3780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24569_length_3780_cov_3.394899, whole genome shotgun sequence.
ACCESSION   DAOPWC010000104 DAOPWC010000000
VERSION     DAOPWC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24569_length_3780_cov_3.394899"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKJ65_03180"
     CDS             complement(<1..268)
                     /locus_tag="PKJ65_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNZ40875.1"
                     /translation="MQLFIITGMSGAGKSYALKTMEDLGFYCMDNIPPSFIPKFVTMV
                     RNNKKIRKVALVTDIRGAEPLGNILVALDELDKQGCKYKLIYLVA"
     gene            complement(268..1155)
                     /gene="murB"
                     /locus_tag="PKJ65_03185"
     CDS             complement(268..1155)
                     /gene="murB"
                     /locus_tag="PKJ65_03185"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ40876.1"
                     /translation="MKTILEKYDFVKFNEPMSNYTSYKTGGIADIVALPENAESLKAI
                     LTILREYNMPVYLFGNCTNILVRDKGIRGTVILTGRLNDIEVEGNFITASAGVSMAKL
                     AAAAYEASLTGAEAVSGIPGSVGGAVSMNAGSYGTSIEDILIRSQYLDENLNICTLQK
                     EQHQFGYRTSFYTDKNYVILNSTFAFTHGDKSSIKRDMLEYARKRKSSQPLEYPSCGS
                     VFKRPVGHFAGKLIQDAGLKGYTVNGAQVSEKHAGFIINKGGATSKDILAIIDYIKSV
                     VYNKYKVNLELEVKIIGEE"
     gene            complement(1139..2095)
                     /gene="hprK"
                     /locus_tag="PKJ65_03190"
     CDS             complement(1139..2095)
                     /gene="hprK"
                     /locus_tag="PKJ65_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNZ40877.1"
                     /translation="MNIPENYDATAHSISVNTIIERYCLDKMYVPENIDKKIIDSAEV
                     YRPGLQLAGYLEGFCPERVQLLGRTEMNFLGTLSREERQKRLDAYMHESIPCIIISNS
                     QPVYDEIIKSARLKRVPVLRTYEGTAVFLAMLISYLNLELAERMTIHGELLELYGVSV
                     LITGHSGVGKSETALELVKRGHRLIADDVVEIRKVSQTTLLGTSPEIIRHFMELRGVG
                     IIDVKGLYGAGSVKDIDSIDLVINIERWDENKKYDRIGMEENYTEYLGIRIPSLTLPI
                     MPGRNMPILMEVAAMDLRQKKMGYNAAKEMYERVNRKFENNT"
     gene            complement(2073..2840)
                     /locus_tag="PKJ65_03195"
     CDS             complement(2073..2840)
                     /locus_tag="PKJ65_03195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ40878.1"
                     /translation="MDFIKAVNAVLDAGALMKDATYRTNAVKKGDKDYVTACDIAIQK
                     SIAQALRHHYPDIPLIAEEKDNRGILSDSVFILDPLDGTTNFLHGYTPSCISLAYVHK
                     EQPVFGIVHDPVADATYIAEKGKGAQLNGKPIRVSNVKNIENALITCETSPYERGDAD
                     MHFDVLKKVFLACMDIRISGSAALDICYVAEGAADAFLTRNLSPWDVAAGICILTEAK
                     GVCTDWKGNPPSMRYKSDILASNGLLQEVLYEYTGKL"
     gene            complement(2840..3127)
                     /locus_tag="PKJ65_03200"
     CDS             complement(2840..3127)
                     /locus_tag="PKJ65_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNZ40879.1"
                     /translation="MKKILILFLTAVILLTLTACQTYLFGVYVSEETGRKYEFMNNEF
                     TCTENDGEISGTYVIKNKTITFKYDGKEDTFSYSRDGAAAVIDGVVYIRGQ"
     gene            complement(3163..>3780)
                     /locus_tag="PKJ65_03205"
     CDS             complement(3163..>3780)
                     /locus_tag="PKJ65_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40880.1"
                     /translation="CRSKGHLDRVTVRNVTGTYRSYGFFINPWFPGDGFGNYGHITFE
                     NIDLRPMDHVYPYRTATLFDIGGNFDCITFKNIHHQNPSDNRPLWVFGLPFHENDLNY
                     APDCRPRIKNVVIDGLTVIQAASDPKVGGYIHVYDRVENLFLKNVIVSSDEGAVKTDA
                     FITFRKFKNCERYGAIGNLVTHDIYMPDVENLTDNPSQVECMCDT"
BASE COUNT          959 a          932 c          708 g         1181 t
ORIGIN      
        1 aagccacaag atatatcagt ttatacttac aaccctgctt atccagttca tccagcgcaa
       61 ctagtatatt tcccagcggt tccgctcccc ttatatcggt aaccagcgca acttttctta
      121 tctttttgtt attgcgtacc atagtaacga acttaggaat aaaggaaggc ggtatgttgt
      181 ccatgcaata gaaacccaag tcttccatgg tcttaagtgc gtatgatttg cccgctccgc
      241 tcattccggt tatgataaac agttgcatta ttcttctccg ataatcttaa cttccaattc
      301 aagatttact ttatatttat tatacacaac actttttata tagtctataa ttgcaagaat
      361 atcttttgag gttgcgcctc ctttattgat gataaaaccg gcatgttttt ccgacacttg
      421 cgccccgttt acggtatagc ctttcaatcc ggcatcctgt ataagttttc cggcgaaatg
      481 tccgaccggt cgcttgaata cgcttccgca ggaaggatat tccaaaggct gagaggactt
      541 tctttttctt gcatactcca gcatatcccg ctttatgcta cttttatcac catgtgtaaa
      601 cgcaaaagtt gaattgagta ttacataatt cttatcggta tagaagcttg ttctgtatcc
      661 gaattgatgc tgttcttttt gtaaagtaca tatatttaag ttttcatcca gatattgaga
      721 ccttatgaga atatcctcta ttgatgtacc gtatgatccg gcattcatgg ataccgctcc
      781 gcctaccgag ccgggtatgc ccgacaccgc ttccgctccg gttaaggatg cttcatatgc
      841 cgctgcggcc agttttgcca tggaaacacc ggccgaggcg gttatgaaat tgccctcaac
      901 ttctatgtca ttcaaccttc ccgttaatat cacagtcccc cttatacctt tatcgcgtac
      961 cagtatgttg gtgcagtttc cgaacaggta aacgggcata ttatattctc tcaatattgt
     1021 aagtatcgct ttgagagatt ctgcattttc cggcaacgcc acaatatccg ctatcccccc
     1081 ggttttatat gaggtatagt tgctcatggg ctcattgaat ttcacaaaat catacttctc
     1141 aagtattgtt ttcaaatttc ctgttcaccc tttcatacat ttctttggct gcattgtatc
     1201 ccatcttctt ttgccttaaa tccattgccg ccacttccat aagtatgggc atgtttcttc
     1261 ccggcattat gggcaatgtc aatgaaggta tccgtattcc cagatactcg gtatagtttt
     1321 cctccatacc tatgcggtcg tattttttat tctcatccca tctctctatg tttataacca
     1381 gatctatact gtctatatct tttaccgaac ccgctccgta aagacctttg acatctataa
     1441 tacccactcc gcgcaattcc atgaaatggc gtattatctc gggcgatgta cctaaaaggg
     1501 tggtctggct taccttacgt atttccacaa cgtcgtcggc tatgaggcgg tgacctcttt
     1561 taaccagttc cagtgcggtc tccgatttgc ccacaccgct gtggcctgtg ataagaacac
     1621 tcacaccgta aagctctaaa agctccccgt gtatggtcat cctttcggcc agttccagat
     1681 tgaggtaact tatgagcatg gctaaaaata ccgccgtacc ttcataagtc cgtaaaaccg
     1741 gtactctctt cagccgtgcc gactttataa tctcatcata taccggctgc gaattggata
     1801 ttataatgca gggtatgctc tcatgcatgt aggcatccag tctcttctgc cgctcctcac
     1861 gggaaagagt acctaaaaag ttcatctcgg ttcttcccaa cagctgcaca cgctccgggc
     1921 agaaaccttc cagatagccc gcaagctgca atcccggtct gtatacttcc gcagaatcta
     1981 tgatcttctt gtcgatattc tccggaacat acatcttatc cagacaatac ctctctataa
     2041 tggtgttcac actgatgctg tgagcagtcg catcataatt ttccggtata ttcataaagc
     2101 acctcctgta acaatccgtt gcttgccaat atatcacttt tatatctcat ggaaggcggg
     2161 ttacctttcc agtcggtaca tacgcctttt gcttcggtca gtatacatat acctgcagcc
     2221 acatcccacg gacttaaatt gcgtgtaaga aaggcatcgg ccgccccttc ggccacataa
     2281 cagatatcca gtgcagccga accgcttata cgtatatcca tgcatgccag gaacactttc
     2341 ttcagcacat cgaaatgcat atcggcatcg cctctttcgt acggactggt ctcgcatgtt
     2401 ataagggcgt tctctatatt cttaacattt gaaaccctta tgggtttacc gttaagttgg
     2461 gcgcctttac ccttttcggc tatataggtg gcatcggcaa ccggatcgtg cactataccg
     2521 aaaaccggct gctctttatg cacataagcc agcgatatgc aggaaggcgt atagccgtgt
     2581 aaaaaattgg tggtgccgtc cagggggtct aatataaaga cggaatcgga taaaataccc
     2641 cggttatcct tctcttctgc gataagcggt atgtcgggat aatggtgccg taaagcttgg
     2701 gctatacttt tttgtatggc tatatcgcat gccgtcacat agtccttatc gccttttttt
     2761 acggcatttg tacgataagt tgcgtctttc atcaatgctc ccgcatccag caccgcatta
     2821 acagctttta taaaatccat tattgtcctc ttatataaac cacaccgtct attacggcgg
     2881 cagctccgtc acgcgagtag gaaaaagtat cttccttacc gtcatatttg aatgtaatgg
     2941 tcttgttttt tataacatag gtcccgctta tttcaccgtc attttccgta catgtaaact
     3001 cattattcat aaactcatat tttcttccgg tctcctcgct tacatacaca ccgaaaagat
     3061 aagtctggca tgcggtaaga gtaagtaata taacggctgt taaaaataag ataagaatct
     3121 ttttcatata ttaatatacc tcttctttct ttccaaatta tatcatgtat cacacataca
     3181 ctccacctgt gacggattat cggtaagatt ctccacatca ggcatatata tgtcatgtgt
     3241 gacaagattg cctatggcac catatctttc acagttttta aatttcctga aagttatgaa
     3301 ggcatcggtc tttacggcac cctcatcgga agatactatc acgttcttta aaaacaggtt
     3361 ttccacacgg tcatatacat gtatatatcc gccaaccttc ggatcggaag cggcctgaat
     3421 tacagttaac ccgtcaatta cgacgttctt gatacgcggc ctgcaatcgg gagcgtaatt
     3481 taagtcgttc tcatggaaag gcaatccgaa cacccacagc ggccggttat ccgaagggtt
     3541 ctgatggtga atgtttttaa atgtaataca gtcgaagttc ccccctatat caaacaaagt
     3601 ggcggttctg taaggatata catggtccat gggacgtaag tctatattct caaaggtaat
     3661 atgtccgtaa ttgccgaaac cgtctccggg aaaccagggg tttatgaaga agccgtagct
     3721 tctgtaagta cctgttacat ttcttaccgt taccctgtcc aagtgtcctt ttgatctgca
//
LOCUS       DAOPWC010000105         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24595_length_3776_cov_3.317657, whole genome shotgun sequence.
ACCESSION   DAOPWC010000105 DAOPWC010000000
VERSION     DAOPWC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24595_length_3776_cov_3.317657"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKJ65_03210"
     CDS             complement(<1..1109)
                     /locus_tag="PKJ65_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNZ40881.1"
                     /translation="MPLDGIAVKCLANELNGICQNGKIQNIYMPEKDEVILGIYANGT
                     SYNVALFLQPSSPGIYFKKEKINNRKTPYGFCMFLRKHIGGGLISEVYAKDYERIIYF
                     DINCTDEFSGNSVKTVIMELTGRNCNLIVVNSSYRILDALRHVDESMSSVRRVMPACE
                     YVLPPIQDKIPIEYVQWEHLLKYADFPLSDAIFRTVLGMAQVFAKEICIRAGVDEAKT
                     VKNISKEEVIQVMSVIKATAQDISANTYSPIVVYSNEKKTITDCYCFDLSVYKNISEF
                     TVKKYPLYIDALSDHLNLKNKRAVFIQASDVVRKSVSAHLAKAEKKLLIYNNNLSDRE
                     KIDKYKLYGELLTANIHAIKQKTDYVELLDYYTDK"
     gene            1229..3445
                     /locus_tag="PKJ65_03215"
     CDS             1229..3445
                     /locus_tag="PKJ65_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNZ40882.1"
                     /translation="MQCLKAAVQNGADAVYLGIKTFSARANADNFTFEELTEAVRYCH
                     LRDVSVYLTLNTLIDDENMQTALNNAKKAYSSGIDGIIIQDLGLAAQIRKMLPDLRLH
                     ASTQMSVIHPKEALLLKSLGFKRIIAAREATLEQIKALVATGIEVEVFGHGSLCVCYS
                     GQCSLSYFNGGKSGNKGGCAQPCRLPYSLFIDNRNISRKNYLSTKDIAVMDDIDRLVS
                     AGVVSLKIEGRMKSPEYVAIITKTYRKALSGTLTDKDRQDAMMAFNREGFSKGYMFSE
                     SGKDMMAYAFSGNTGIRIGTVKKINKEKNLIQIESNKELINGDGISFPDSSIGTYVNI
                     VRKNGNLYGLICRNKLPEPNEPVYLTYDKQLTEQAQKSYADDFARKIPINGHFVSKVG
                     QKASLTVTDNSGNTYTSYSDIETQAANNPNADDDILKNLNKTGNTVFEFSSLTKDTDT
                     ATFLPAKTVNALRRDALNGLAELRQSIKNRNSKTELFPKYENLSLSSESLNIEKTEKR
                     NTPVNRDISNKKTGISLFFFEDRLDFDIADIDADRIYVPSTMYKRYLHDDRVYLYIPI
                     GANPQDTAGNRQRIISSSMGILDIPADKVLDAGINCLNTYTVDFLSGYKDIKAIHLSY
                     ELPVENIRRFANFNGNISLEYLVYGRVAVMKTKYRIKDIFTDTDTQEIYLLDRHKKRI
                     DIVTYPDCDTTYLLGAFTVNNLLHKDLIRASGADTLRINVYKETADEIKDIIKRAV"
     gene            complement(3446..>3776)
                     /locus_tag="PKJ65_03220"
     CDS             complement(3446..>3776)
                     /locus_tag="PKJ65_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ40883.1"
                     /translation="GKKAAVALNPATDIRVLEYVLPMLDMVLIMSVNPGFGGQKYIPY
                     STEKIKALKKMIDKMGLKTDIQVDGGVTVDNCREIKAAGANVLVAGSAIFQSKDIKET
                     IRKLIEY"
BASE COUNT         1194 a          759 c          722 g         1101 t
ORIGIN      
        1 atcttgtcgg tgtaataatc taaaagctcc acataatcgg tcttttgttt aatcgcatga
       61 atattggctg tcaaaagttc tccgtacagt ttgtatttat ctatcttttc tctatccgaa
      121 agattgttgt tatatattaa aagttttttc tccgcttttg ccagatgggc cgataccgat
      181 tttctgacca catcggatgc ttgtataaaa acagctcttt tattctttaa gtttaaatgg
      241 tcgcttaatg catcaatata taaaggatat ttcttaaccg taaactccga tatattttta
      301 tacactgaaa gatcaaaaca gtaacaatcg gttatggtct ttttttcatt gctgtaaacg
      361 acaatcggag aatatgtgtt tgccgatatg tcttgtgctg ttgctttaat aacggacata
      421 acctgtatca cttcttcttt gctgatattc ttaacagtct ttgcttcatc tactccggct
      481 cttatacata tctcttttgc aaaaacctgt gccataccta aaaccgttcg gaatattgca
      541 tcacttaagg gaaaatccgc atatttaagt aaatgctccc actgcacata ttctatcggt
      601 atcttgtcct gtatgggagg aagcacatac tcgcatgccg gcattactct tcttaccgaa
      661 gacatggatt cgtccacatg ccttaaagcg tccaatattc tgtatgaaga attgacgact
      721 atgaggttgc agttgcgacc ggtcagctcc attatcacgg tcttaaccga attgccgctg
      781 aattcatcgg tgcaatttat atcaaagtat attattctct cataatcttt tgcataaacc
      841 tcggatatca atcctcctcc tatgtgcttt cgcaagaaca tgcagaaacc gtacggggtt
      901 ttacgattgt ttatcttttc ttttttgaaa tatattcccg gtgaagaagg ctgcagaaac
      961 aatgccacat tgtaagatgt gccgtttgcg tatatgccca gtataacctc atctttttcc
     1021 ggcatgtaaa tattctgaat cttcccgttt tggcatatac cgttcaattc atttgcaaga
     1081 cactttactg caattccgtc cagcggcatg atatcctccc atctcaaaac attgccgttt
     1141 tatattatat atattatcat aatatccgta ctttaatact tggtacagga aagaggacac
     1201 tttggaatta ttatctccgg cgggaaatat gcaatgtctt aaagccgcag tacaaaacgg
     1261 agccgatgcc gtttatctcg ggataaagac tttcagtgca agagcaaatg ccgacaattt
     1321 tacatttgaa gaattgacgg aagctgtaag atactgtcat cttcgtgatg tatccgttta
     1381 tctgacactt aacacactta tagatgacga gaatatgcaa accgccctca ataatgcaaa
     1441 gaaagcatat tcatcaggta tagacgggat tattattcag gacttagggc ttgctgcaca
     1501 gataagaaaa atgcttcccg acctgagact gcacgcatcc acacagatga gcgttatcca
     1561 tccgaaagaa gcattgttgc tcaagagttt gggatttaaa aggataatag cggcaagaga
     1621 agccacctta gagcagataa aggcgcttgt tgccacgggt attgaagtgg aggttttcgg
     1681 tcacggctcc ttatgtgtat gttactcggg gcagtgcagt ttaagttact ttaacggagg
     1741 caaatcggga aataaaggcg gttgtgccca gccctgcaga cttccctact cactgtttat
     1801 agataatcgt aatatctccc gtaaaaacta tctcagcacc aaagacatag cggtaatgga
     1861 tgatatagac aggcttgtat cggcgggtgt tgtatctttg aaaattgaag gcagaatgaa
     1921 aagcccggaa tatgtggcga ttataacaaa gacatacaga aaggctcttt cgggtactct
     1981 tacagataaa gacagacagg atgccatgat ggcattcaac agggaaggct tttccaaagg
     2041 atatatgttt tccgaatccg gtaaagatat gatggcatat gctttttccg gaaataccgg
     2101 tataaggata gggaccgtca agaaaataaa caaagagaaa aacctgatac agattgaaag
     2161 caacaaggaa cttataaacg gtgacgggat aagttttccc gacagctcta tagggacata
     2221 tgtcaatatt gtaagaaaaa acggcaatct gtacggttta atatgcagaa ataaactccc
     2281 cgaaccaaat gagcctgtat acctgacata tgataagcaa ctgaccgaac aggcacaaaa
     2341 atcctatgcc gatgattttg cgcgcaaaat acccataaac ggtcattttg tatcgaaagt
     2401 cgggcagaaa gcaagcttaa ccgtaaccga taacagcggc aacacctata cctcttattc
     2461 cgatatagag acgcaagcgg caaataaccc taatgccgac gatgacatat tgaagaatct
     2521 caacaaaacg ggaaatacgg tatttgaatt ctcctctctc acgaaagata ccgataccgc
     2581 tacattcctt cccgctaaaa cggtaaatgc tttaagaaga gatgctttga acggattagc
     2641 agaattaagg cagagcataa aaaacagaaa tagtaaaacc gaactgtttc ccaagtatga
     2701 aaacctatcg ttatcttcgg aaagtttaaa tatagagaag acagaaaaaa gaaatactcc
     2761 ggtaaacaga gatataagta ataaaaaaac aggtatatcc ttattcttct ttgaggaccg
     2821 attggatttt gatatagcgg atattgatgc agacaggata tatgtacctt ctaccatgta
     2881 taaaagatat cttcacgatg acagagtgta cttgtatata cccataggag ccaatcctca
     2941 agatacggca ggcaatcggc aaagaattat ttcctcttcc atggggattt tagatattcc
     3001 tgcggataaa gtactggatg cgggaataaa ctgcttgaat acttatactg tagattttct
     3061 ttcggggtat aaggacataa aagccataca cctttcatat gaattacctg tcgaaaatat
     3121 aagaagattt gcaaacttta acgggaatat aagtctggag taccttgtat acggtagagt
     3181 tgccgtaatg aaaaccaaat accgtataaa ggatatattc accgataccg atacacagga
     3241 aatatatctt ttagaccgac ataaaaaacg cattgacata gttacatatc ccgactgtga
     3301 taccacatat cttttaggtg catttacggt aaataatctt ttgcataaag atttaataag
     3361 agcatccggt gcggatactc tcagaattaa tgtttataaa gaaacagcgg atgaaattaa
     3421 agatataatc aaaagagcag tataatcagt attctataag ttttctaata gtttctttaa
     3481 tatctttcga ctggaatatt gccgatcccg caacaagaac attggctccc gcagccttta
     3541 tctcacggca gttatccacc gttacgccgc cgtcaacctg aatgtcggtt ttcagcccca
     3601 ttttgtctat cattttctta agagctttta ttttctccgt gctgtacggt atatactttt
     3661 gtcctccgaa accgggattt acactcatta taagcaccat gtccaacata ggcagaacat
     3721 attccaaaac ccttatatcg gtggcgggat taagagcaac cgccgctttc ttgcct
//
LOCUS       DAOPWC010000106         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24667_length_3769_cov_3.957458, whole genome shotgun sequence.
ACCESSION   DAOPWC010000106 DAOPWC010000000
VERSION     DAOPWC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24667_length_3769_cov_3.957458"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKJ65_03225"
     CDS             complement(<1..652)
                     /locus_tag="PKJ65_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ40884.1"
                     /translation="MYEKNIKEMIEMSRKVGSMPDYTQGGGGNTSVKLDDRLMAVKAS
                     GCRLCDITATDGFVVVDYKSVRHYFDTVNLSEKRDFYKESSQFVKDNMVKLEGLNDGR
                     PSIEAGFHSLLEKYVIHTHSVYSNLLCCSRQGESIAKQLFEKSPYRYLWLPYIAPGFY
                     LTLEIQKKIKTMGCIPSVIFMGSHGVIVTGKTMAEAEKINEYVSERIKERLNITEPY"
     gene            complement(707..2185)
                     /locus_tag="PKJ65_03230"
     CDS             complement(707..2185)
                     /locus_tag="PKJ65_03230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNZ40885.1"
                     /translation="MIKVLAYDFGASSGRAILGVYDGKKIHIEELHRFSNDPVNLNGT
                     LYWDILRLFHEMKVGLIEVKKAGHGDIESIGIDTWGVDFGLIDKDGRLLENPVHYRDK
                     RTDGIMEEVWDKVGKRHLYERTGTQFMQFNTLYQLYSLVKNRPELLKRADRLLFMPDL
                     LSYFLTGKKVAEFSMASTAQMLNTRTGDWDYELLEKLGIPVRILEKVTDSATVIGKLS
                     EQVCNETGLQPISVTAVAGHDTGSAMIATPADTDLFAFLSSGTWSLLGSELPKPIVND
                     LSYKHEYSNEGGINRTARLIKNIMGLWIYQECRRQWNKEGDLASFDELEAAARKAKPF
                     ACLIDVDHQDFYPPGDMPARVADFCRRTGQYVPKNKAEIVRCIMESLALKYRYAFDGL
                     CEIVGSRIPKLHMVGGGCKNAMISQFTADALGIEVTAGPVEATAVGNIAAQLIALGKI
                     KDLKQARIIIKESFEVKSYFPENRELWEEKYDYYLKNILNKG"
     gene            complement(2224..2955)
                     /locus_tag="PKJ65_03235"
     CDS             complement(2224..2955)
                     /locus_tag="PKJ65_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ40886.1"
                     /translation="MSYQELCRKRDYVNGKLPELSPEKIEELEKQFECDYAYESIKIK
                     GSELSRDQVMAILNDKADDLEKTRDVQGTINHSRAFKYMKMYLKKNAYLDEEAIKEIN
                     AIVVEDISVGGIYRNVNLIIPGRKHVPPSPEKAYYKMKKYILDLPYLQYDTVTEKAIY
                     AYAMFMKIHPFIDGNSRTGKLIMNYVLMKEGFLPIYIPQNRYIEYVRAFELFASEDNL
                     EPLAKLAFEFENAQLDIYIKAIDKA"
     gene            complement(2973..>3769)
                     /locus_tag="PKJ65_03240"
     CDS             complement(2973..>3769)
                     /locus_tag="PKJ65_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ40887.1"
                     /translation="TIQDFMAPYRAARNDRNRAMCEKINQVLNKYDISLDFDKEVAPL
                     SLYHEGGTVTERHISYALALKMIERFKRGKALIDFYKTTLNLPLSLKNEALLSDVSNP
                     VYEYDLLGAIKSDLISLFYIDAYDECPDIHDVISLSRKTKAISAYAYLGDVASSVTGD
                     KKPQKFEDSYLDKLFEVLKQLDFDAVTYMPSRNTKEQLIRVRTLCERYGFFQISGEDI
                     NSPRQSFVCKAMRDPFFDNLRESTYALINHERQATEDITKAMFYNK"
BASE COUNT          940 a          896 c          669 g         1264 t
ORIGIN      
        1 cgtacggttc tgttatattc aatctttctt ttattctctc ggaaacatat tcatttatct
       61 tttccgcttc ggccatagtc ttgccggtta cgataacgcc gtggcttccc atgaatataa
      121 cggagggaat acatcccatt gtctttatct tcttttgtat ttccagtgtg agataaaaac
      181 ccggagcaat gtacggaagc cacaggtatc tgtaaggtga tttctcaaag agttgctttg
      241 ctatactttc tccctgcctt gaacagcata aaagattgct gtaaaccgaa tgggtatgga
      301 taacatactt ttctaaaagc gaatggaatc ccgcttctat cgacggtctt ccgtcattga
      361 gcccttctaa ttttaccata ttgtctttaa cgaactgcga actttcttta tagaaatccc
      421 ttttttcgct tagattcacg gtgtcaaaat agtgacggac ggatttataa tctaccacaa
      481 caaaaccgtc ggttgcggtt atgtcgcaaa gtctgcaacc ggatgctttt accgccataa
      541 gccggtcatc caatttcacc gaagtgtttc cgccgcctcc ctgtgtgtag tcaggcatac
      601 ttcccacctt acgggacatt tctatcattt cttttatgtt tttttcgtac ataataatct
      661 ccttcatgtt ttttactgac gggattttat tgattgtata tctattttag cccttgttca
      721 atatgttttt caggtaatag tcatattttt cttcccaaag ctctctgttt tcggggaaat
      781 atgacttaac ttcaaagctt tcttttataa ttattctagc ctgcttgagg tcttttatct
      841 tgcctaaagc tataagttgc gccgctatat tcccgacagc cgttgcttca acgggacctg
      901 ccgtaacctc tatacccaaa gcatccgccg taaattgcga tatcattgca ttcttgcagc
      961 cgcctcccac catatgaagt ttgggtatcc tgcttcccac tatttcacaa agtccgtcaa
     1021 acgcataacg gtatttcaat gccaggcttt ccattatgca tcgtactatc tcggctttgt
     1081 tcttgggaac atactgaccg gttcgtctgc agaaatccgc aactctggca ggcatatctc
     1141 ccgggggata aaagtcctga tggtccacat caatcaagca tgcaaaaggc tttgctttcc
     1201 gtgcagccgc ctccagctcg tcaaaggatg caagatcccc ttctttgttc cattgtcttc
     1261 tgcattcctg atatatccat aagcccataa tgttttttat aagtcttgcc gttctgttta
     1321 ttccgccttc gttggaatac tcatgtttat atgaaagatc attcactatc ggcttgggca
     1381 gttcggaacc cagtaaggac catgttcccg aagaaagaaa tgcaaacaga tcggtgtctg
     1441 cgggagtggc tatcatggcc gatccggtgt catgtcccgc tacggcggta acggatatcg
     1501 gttgcagtcc tgtctcatta caaacctgct ctgaaagttt tcctatgact gttgcactgt
     1561 ctgttacctt ttccaatatc cttacaggta ttcccagttt ttccagcaat tcgtaatccc
     1621 agtcaccggt acgggtattc agcatctgtg cagtgcttgc catggagaat tccgccactt
     1681 ttttgccggt gagaaaatat gaaagcaaat ccggcatgaa aagaagcctg tccgctcttt
     1741 ttaacaactc cggccggttt tttaccaacg aatacagctg ataaagtgta ttgaattgca
     1801 taaactgcgt tcccgttctt tcgtaaagat gtcgctttcc taccttatcc cacacttcct
     1861 ccataatacc gtcggttctt ttatctctgt aatgtacggg attttctaaa agccttccgt
     1921 ctttatcaat aagaccgaaa tccacccccc acgtatcaat tccgatactc tctatatcac
     1981 cgtgacctgc tttctttact tcgataagac ctaccttcat ttcatggaaa agacgcaaaa
     2041 tatcccagta cagggtaccg ttcagattca cgggatcatt ggaaaacctg tgcaattctt
     2101 ctatgtgtat cttctttccg tcatatactc ctaagattgc tcttccgctg cttgcgccga
     2161 aatcatatgc caataccttt atcatcatta acctccaatg attttagtgt ataagtatct
     2221 gttttatgcc ttgtctatcg ctttaatata tatgtccagc tgtgcgtttt caaattcaaa
     2281 agccaacttg gctaaaggct cgagattatc ttccgaagca aacaactcga atgcacgcac
     2341 atactcgata tatctgttct gcggtatgta tatgggcagg aaaccttcct tcataaggac
     2401 ataattcatg ataagctttc cggtcctgct gtttccgtca ataaaaggat gaatcttcat
     2461 gaacatggca tatgcataaa tggctttttc cgtaaccgta tcatattgca gatagggcag
     2521 atcgagaata tattttttca ttttgtaata tgccttttcc ggtgacggcg gaacatgctt
     2581 tcttccggga atgataagat tgacattgcg atatataccg cctacggaaa tgtcttccac
     2641 aactatggca tttatctctt ttattgcttc ctcatcaaga tatgcgttct tctttaagta
     2701 cattttcata tacttgaaag ctctgctgtg gtttatcgtt ccctggacat cccttgtttt
     2761 ctccaagtcg tcggctttat catttaagat agccatgacc tgatccctcg acagttcact
     2821 tcctttaatc tttatggatt cgtaagcata gtcacactcg aattgttttt ccaattcctc
     2881 gattttttca ggggataatt cgggcaattt gccgtttacg taatctcttt ttctgcacaa
     2941 ttcctggtag ctcaattgcg gacacctctt tcttatttat tataaaacat agccttagtt
     3001 atatcttcgg tcgcttgccg ttcgtgattt atcaaggcat atgttgattc ccttaaatta
     3061 tcgaaaaacg gatctctcat ggctttgcac acaaaactct gtctcggtga gtttatatcc
     3121 tctccgctta tctggaaaaa tccgtaccgc tcacataacg ttctgaccct tatcagttgc
     3181 tctttggtat ttctcgaggg catatatgtc accgcatcga aatccagttg ctttaacact
     3241 tcaaaaagct tatccagata agagtcctca aatttctgcg gcttcttgtc tccggtcacc
     3301 gaacttgcca catctcctaa atatgcatat gccgatatgg ctttggtctt tctgctcaaa
     3361 gatattacat cgtgtatatc gggacactcg tcataagcat ctatgtagaa cagtgaaatc
     3421 aaatcacttt ttatcgctcc caaaaggtca tactcataca ccggattgga cacatcgctc
     3481 aataaagctt cattcttaag gcttaaaggt aaatttaaag tagtcttgta gaaatctata
     3541 agtgcttttc ctcttttgaa acgttctatc attttaagtg caagtgcata agatatgtgt
     3601 ctttcggtta ccgttccgcc ttcatgatac aaagataacg gagcgacctc tttatcaaaa
     3661 tccaatgaaa tatcatattt gttcaagacc tgatttatct tttcacacat agctcggttg
     3721 cggtcatttc tggccgctct gtacggcgcc atgaaatcct gtattgtat
//
LOCUS       DAOPWC010000107         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24752_length_3760_cov_2.811876, whole genome shotgun sequence.
ACCESSION   DAOPWC010000107 DAOPWC010000000
VERSION     DAOPWC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24752_length_3760_cov_2.811876"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..359)
                     /locus_tag="PKJ65_03245"
     CDS             complement(33..359)
                     /locus_tag="PKJ65_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HNZ40888.1"
                     /translation="MQVTPLQTNRSLGRFILLSIVTLGIYGIVYFYKVGRDLNTVCSP
                     YDGKKTMHFVLLALLIAPLTAGIGGIVWIHKMSNRVGWALTHRNIAYTFNSNTYWLWD
                     VLGRLS"
     gene            complement(457..1662)
                     /locus_tag="PKJ65_03250"
     CDS             complement(457..1662)
                     /locus_tag="PKJ65_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNZ40889.1"
                     /translation="MEMQMPSKQVIQKKKELVLDRIYNYHLKKMAGSEGNVFLISDTY
                     PGVWLEHVYDAVSWVNYDKTDPTVSVHQIRLFLDRQKPDGQFPCYVIDPEKGNKFKNK
                     VGYSQIQECLSFARLCLETCIQNNDTELLAECYDRCKKWDRWLTRNRMTMKTGLIEMF
                     CGYDTGHDNSGRFANIKYKGNISEDAKDYPHDDHSLPHITPDMNAVFYGSMTALAVMA
                     GKLGLEQEAHDWKQAAREVKKKILEICYDEQDDFFYDVDRHGNKIKCKSISITNLFCE
                     NVLDVDMAKSIFSKYLDNPKEFKTPYPYPAVSISDPLWIQNRQGNSWGFYSQGLVALR
                     SLRWMDKYGFSEQLEDNMKKWITAWCLTDKLNFGQELHPITGEPSDCGNWYSSTMLYF
                     IHSLRRLGL"
     gene            1812..2798
                     /locus_tag="PKJ65_03255"
     CDS             1812..2798
                     /locus_tag="PKJ65_03255"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNZ40890.1"
                     /translation="MIGKELYHKMHMEFQVLIHPDELSYEWIDRVISSGIDAIGIHPV
                     GGYRARESLADLVERFKDNNFTDLIDYASDNGIKIEYEMHAASYLMPRELFSSHPEYF
                     RVDEAGNRNNDANFCVSNQEALQYVSNAAAILASGLYKSSERYYFWLDDVKNKKCMCE
                     KCRNLSMSDQQMIFINAALKEIQKSNRKAKMAYLAYFDCITPPQKIKPVKGVFAEYAP
                     MGKYVSETQTHKNSKINEESDINNLFSVFSKSNSKVLEYWLDNSMFSNWTKPEKKFVP
                     DNKMIKEDIEKYHSLGFEYISSFACYLGPEYTKLHGQPDLSAFYEVVKSKKH"
     gene            complement(2810..3457)
                     /locus_tag="PKJ65_03260"
     CDS             complement(2810..3457)
                     /locus_tag="PKJ65_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40891.1"
                     /translation="MGLFATLLSLIFKDEEIDYSEIKSVMVIDITQLYTTKYNYGYSS
                     GWKWGDGRSIMMPSEVPDGGQYTFSVTFKDGSKKIIKARSDTEKCNKLLQFVYDPPGK
                     EKSSTSQLQIEDKENKNHYPVLGKNQLPNGIYVIGKDIPPGTYDFKCIWGDCYLVKIG
                     KGETDWNCTNYYECIGADSETRVCLNVTCEEGEKIKVDGNAILEISKSKPVEIDL"
     gene            complement(3597..3681)
                     /locus_tag="PKJ65_03265"
     tRNA            complement(3597..3681)
                     /locus_tag="PKJ65_03265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3645..3647),aa:Tyr,seq:gta)
BASE COUNT         1116 a          760 c          713 g         1171 t
ORIGIN      
        1 ggaaagtcta tgcaaataaa tgaaaggtcc tactatgata agcgtcctaa tacatcccaa
       61 agccaatatg tattggaatt gaatgtataa gctatgtttc tgtgagtaag agcccagccg
      121 acacgattac tcattttgtg tatccagact ataccgccta ttcctgctgt aagaggagct
      181 atcaacagtg ccaaaagaac aaagtgcatt gttttcttac cgtcatacgg agaacagaca
      241 gtgttcagat ctctgcctac tttgtaaaaa tatacaatac cgtatattcc gagtgtgacg
      301 atactgagga gaataaatct tcctaaactt ctatttgttt gtagtggtgt tacttgcatt
      361 acatataccc cctttgttat tttatataac cacatgttta tactaaaagt atacttttat
      421 taaaaacttg tgtcaatgtt tttacacaat tttccgttat agtcccaatc ttcgcaatga
      481 atgtataaaa tacagcattg tggaggaata ccagttaccg caatcggaag gctctcccgt
      541 aatggggtga agttcctgcc cgaaattcag tttatcggtc aggcaccatg ccgttatcca
      601 ttttttcatg ttgtcttcca gttgctcgga gaaaccgtat ttatccatcc atctcaatga
      661 ccgaagagct accagtccct gtgaataaaa gccccatgaa ttaccttgtc tgttctgtat
      721 ccataacggg tcggaaatag agacggcggg gtaggggtaa ggagtcttga attccttcgg
      781 gttatcaagg tatttgctga aaatactttt agccatatct acatccaata cattttcaca
      841 gaacagattg gttattgaaa ttgatttgca ctttatttta ttcccgtgcc tgtctacatc
      901 ataaaaaaag tcatcctgct cgtcataaca tatctccagt atttttttct tgacctcacg
      961 tgcggcttgt ttccaatcat gagcttcctg ttcaagtccc agttttccgg ccattacagc
     1021 aagtgccgtc atggacccgt aaaaaaccgc attcatatcg ggtgttatat gaggaagcga
     1081 gtggtcgtca tgcgggtaat cctttgcatc ttcggagatg ttgcctttat acttgatatt
     1141 tgcaaacctt ccgctgttat catgtcccgt gtcatatccg cagaacattt caatcagacc
     1201 ggttttcatg gtcattctgt ttcgggtaag ccagcggtcc cactttttgc atctgtcata
     1261 acactccgcc agaagttccg tgtcattatt ctgtatgcat gtctccaaac acagtctggc
     1321 aaaggacaga cattcctgaa tctggctgta tcctaccttg ttcttgaatt tgttaccttt
     1381 ttcgggatct attacatagc aggggaactg tccgtcgggt ttttgcctgt ccagaaaaag
     1441 acgaatctga tgcaccgata cggtcggatc ggtcttatcg taattaaccc aggataccgc
     1501 atcatagaca tgttccaacc atacaccggg atatgtgtcg gatatcagaa agacattgcc
     1561 ttctgaaccc gccatttttt taagatggta gttatagatt ctgtccagaa ccaattcttt
     1621 ctttttctga attacttgtt ttgaaggcat ctgcatttcc atttattttc ctcaagtttc
     1681 ttaattttga ccattataat tctaattaat aagagatttt gtgtcaatga ggaccaagtc
     1741 gaatttgtta ccttgaaagt ctatgtttat cagccggtaa acggtaaaag tgtataatat
     1801 agataaggag agtgattggc aaagaactat atcataaaat gcacatggaa tttcaggttt
     1861 taatacatcc cgacgaatta tcatatgagt ggattgaccg ggttatatca agcggtattg
     1921 atgcaatagg tattcacccc gtgggaggat acagggctcg ggagagtctt gccgatttgg
     1981 tggaaagatt caaagacaat aattttaccg accttataga ttatgcatcg gacaacggta
     2041 taaaaataga gtacgaaatg catgcggctt cctaccttat gccaagagag cttttttcca
     2101 gtcaccccga gtatttccga gtagatgaag caggtaacag aaacaacgat gcaaacttct
     2161 gtgtttcaaa ccaagaagcg ctgcaatatg tgagcaatgc ggcagcaatc ctggcatccg
     2221 ggctttacaa aagttcggaa agatactatt tctggttgga tgatgttaag aataagaagt
     2281 gtatgtgtga gaaatgccgc aacctttcca tgtccgacca acaaatgata ttcattaatg
     2341 cggcattaaa agaaatccaa aaaagtaatc ggaaagccaa gatggcatat cttgcatatt
     2401 ttgactgtat tacacctccg caaaagataa aacctgttaa aggagttttt gcggaatatg
     2461 ctcctatggg gaaatatgtg tccgaaacgc aaacacataa aaactctaaa ataaatgaag
     2521 aaagcgatat caacaatctg ttttccgttt tctcaaagag caactccaaa gtgttggaat
     2581 attggctgga taactccatg ttttccaact ggaccaagcc ggagaagaag tttgttccgg
     2641 acaataaaat gataaaagaa gatatagaga aatatcattc tttaggattt gaatatattt
     2701 caagttttgc ctgctacctt ggaccggaat acacaaagct gcacgggcaa ccggatttgt
     2761 cggcttttta tgaggttgtt aaaagtaaaa aacattaata tttaaaaaat tataaatcaa
     2821 tctcaacagg tttagatttg gatatctcta atattgcatt accgtcaacc ttgattttct
     2881 cgccttcctc acatgtcaca ttcaaacata ccctggtttc agaatcagct cctatacact
     2941 cataatagtt cgtacaattc cagtctgttt cacctttacc gatttttact aaatagcagt
     3001 ctccccatat gcatttgaaa tcatatgttc caggaggaat atcttttcct atgacatata
     3061 taccgttcgg aagttggttt ttcccaagca cgggataatg gtttttattc tctttatcct
     3121 ctatttgcag ttgcgatgtg cttgattttt cttttccggg aggatcataa acaaattgaa
     3181 gtaatttatt gcatttttca gtatcgcttc gtgctttaat aattttttta ctgccgtctt
     3241 tgaatgtaac actaaatgta tattgaccgc cgtccggcac ttcagacggc atcataatgg
     3301 atcttccgtc gccccacttc caaccggatg aatagccgta attatattta gtggtataaa
     3361 gttgtgtaat gtctataacc ataacggatt ttatttcgct gtaatcaatt tcttcatctt
     3421 taaatatcaa acttaataaa gtagcaaaga gtcccattat gttacctgcc ttatactgat
     3481 aaccaaatat taacataaaa atgcagagaa gagaacagaa ggtttgatga ataaacattg
     3541 tataacaata aatgttcaat aacagaagaa aaaagaacac ctcacggtgt tctttttggt
     3601 ggagagagat ggatttgaac catcgaaatc atagataaca gatttacagt ctgccccctt
     3661 tggccactcg ggaacctctc cgtacactgt tttatattgg cgacccggat ggggctcgaa
     3721 cccacgacct ccagcgtgac aggctggcat tctaaccaac
//
LOCUS       DAOPWC010000108         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24914_length_3742_cov_3.754272, whole genome shotgun sequence.
ACCESSION   DAOPWC010000108 DAOPWC010000000
VERSION     DAOPWC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24914_length_3742_cov_3.754272"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PKJ65_03270"
     CDS             complement(<1..136)
                     /locus_tag="PKJ65_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40892.1"
                     /translation="MNRYYESESMELKAGYTDTVVREIVSFLNTEGGVIYIGIEDDGT
                     V"
     gene            366..>759
                     /locus_tag="PKJ65_03275"
     CDS             366..>759
                     /locus_tag="PKJ65_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40893.1"
                     /translation="MEKANKIKKTCMIFQTLLLMWFFLDMTGVSLGDKCLVTQSYRED
                     GVFFLIYLFTVILFIVKVHIGKWLVVVWTSLWFIAQFLSHEWYTIFNCGIMGSVEGKI
                     KYFSGTLQWIRIEGKYIPDIYHTVLHILI"
     assembly_gap    760..769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(884..2053)
                     /gene="nagA"
                     /locus_tag="PKJ65_03280"
     CDS             complement(884..2053)
                     /gene="nagA"
                     /locus_tag="PKJ65_03280"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNZ40894.1"
                     /translation="MRTIIYNGKLILKDVISERGHVEITDGVITNVSQHSPDKDLLSQ
                     ADNIIDAKGMYISPGFIDLHTHGGGGYDFGVNSTDSYIVPCEIHARHGTTSLYPTISS
                     ATFETMEEAVVLYKEAKYINHSGATMRGLHFEGPYFSPAQKGAQSEEHLRTPKPFEYL
                     KILNMTDDIKRWSSACELDGMQQFTYELKKRGILPSIGHCDATYEEVKQARKWGFSLI
                     THLYSCCSTITRDKGYRILGVTEAAYLIEDMDVEIICDGHHLPDGLIQMVYRFIGPDR
                     TAIITDCLSYAGLDAPDGTLLGDRFVVEGGVAKLLDRSAFAGSIATFDMLIRTALKAG
                     LPLKDIIKMSSITPARIMGIDKQTGSLEKGKQADVIVFDKDINMHAVFVKGKRVL"
     gene            2144..2902
                     /locus_tag="PKJ65_03285"
     CDS             2144..2902
                     /locus_tag="PKJ65_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ40895.1"
                     /translation="MKLAAQLYTLREYTQTETDYYNTCVKVKNMGYSSVQLSGAGPME
                     AKTVKSISDELNLPVTATHIPHTMFVNELDKVIQNHKIMGCKYVGLGYMPKEYWESVS
                     TLNEFITTYSKVSEALDKQGLRFVYHNHHFEFFKFKGKTMMDIILENTPSCFFFLLDT
                     YWIQAAGANPVYWIKKLKGRGDYIHFKDMGIGSDYKTTFVPVGQGNLDWQNIIQACRY
                     AEVKYIAVEQDTCEGSPFDALKTSYDYLSSLGLK"
     gene            2913..>3742
                     /locus_tag="PKJ65_03290"
     CDS             2913..>3742
                     /locus_tag="PKJ65_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40896.1"
                     /translation="MTEVRFGIIGYGNIAGTHLSFFDKNEIPDARVTAIFDTDKSRAD
                     KAREKYKGKIAVFDDEEEFFSSGLFDAILICSPHYHHPHYAIKGFEHNLHVLCEKPAG
                     VYTKQVREMNEAYRHHKHLVFAMMYNQRTNPLYRKVRDMLLNGEYGEIRRCVWIITDW
                     FRTQRYYDSGGWRATWAYEGGGVLLNQDPHQLDLWQWICGMPVRVRAFLGFGKFHDIE
                     VEDDVTAYVEYPNGATGVFITSTGEAPGTNRFEISLDRGKIVIENDKICAYKLKEDLS
                     "
BASE COUNT         1130 a          727 c          775 g         1100 t
ORIGIN      
        1 ttacggttcc gtcatcttcg ataccaatgt atatcactcc gccttcggtg ttaagaaaag
       61 aaactatttc ccgcacaacg gtatccgtat atccggcttt caattccatt gattccgatt
      121 cataatacct gttcattttt tatcaccgcc tttcgcatag ataataaacc aacagtgcca
      181 taatgtcaag cattagtgcc taattggcac taatgctatg ttgtcagtgc catatttgac
      241 caaaagacaa ggtatatggc agatatttac ttgttgtaag cattaaatgt atattatagg
      301 cctttctaat atgtataata aagaggacct atttaatcat aaaatagaat tttttgagag
      361 gtaatatgga aaaagcaaat aagattaaaa aaacttgtat gatatttcaa acactccttt
      421 taatgtggtt tttccttgac atgacaggag tttccttagg tgataagtgt ttagttacac
      481 agtcatatag ggaggatgga gtgttctttt tgatttatct ttttacagtt atattattta
      541 ttgtgaaagt tcatattggg aagtggcttg ttgttgtttg gacttcttta tggtttatag
      601 ctcaattttt gagccatgaa tggtatacaa tttttaattg cggcattatg ggcagtgtag
      661 aaggaaaaat aaaatatttt tcaggtacac ttcaatggat aaggattgaa ggaaaatata
      721 taccagatat ttatcataca gtattacata tccttatctn nnnnnnnnna tcttattagt
      781 tctaatcagc acaattatct atattgtaaa aagtaagaaa aaggtggaag tccaaattta
      841 gtttttagtc aattccgact tatgtagagt tttaggtgca atattacaac acacgcttgc
      901 cctttacaaa caccgcatgc atattaatat ccttatcaaa aacaatgacg tccgcttgct
      961 tgcctttttc aagtgaaccg gtctgtttgt caatgcccat aattcttgca ggtgttatgg
     1021 aagacatctt tataatatcc ttcagaggta accccgcctt caatgccgtc cgtataagca
     1081 tatcaaatgt ggcaatggag cccgcaaaag ccgacctgtc caaaagcttg gccactccgc
     1141 cttccacaac aaatctgtca cccagtaaag tgccgtccgg ggcatccagt cccgcatagg
     1201 aaaggcagtc ggtaataatg gccgttctgt ccggtcctat gaatctgtat accatctgaa
     1261 taagaccgtc gggaagatga tgtccgtcac agataatctc cacatccatg tcctctatca
     1321 gataagcggc ttcggttact cctaaaatac ggtatccttt atctcttgtt attgtggaac
     1381 aacaggaata cagatgggtt atgagggaaa atccccattt ccgtgcttgc ttaacctctt
     1441 catatgtggc atcgcagtgt cctattgacg gcagtattcc tctttttttc aattcatacg
     1501 tgaattgctg cataccgtcc aactcacatg ccgaagacca cctctttata tcatcggtca
     1561 tgttaagtat tttcagatat tcaaaaggct taggtgttct taaatgctct tcactctgtg
     1621 cacctttctg tgcaggcgaa aagtacggac cttcaaagtg cagccctctc attgttgctc
     1681 ccgaatgatt tatgtacttt gcttctttgt ataacacaac agcttcttcc atggtttcaa
     1741 aagttgccga tgaaattgtg ggatacagac ttgtggtacc gtggcgtgca tgaatttcgc
     1801 aggggacgat ataggaatcc gtgctgttta ccccgaaatc ataacctccc ccgccatggg
     1861 tgtgtaaatc aataaatccc ggagagatat acatgccctt tgcatctatg atattgtccg
     1921 cctgtgataa taaatcttta tcggggctat gttgtgatac atttgtaata actccgtcgg
     1981 ttatttccac atgcccccgc tcggatatta catccttcag tatgagtttt ccgttataaa
     2041 taattgtgcg cataaatttt ccttccttcc cccattatat tatttaaccg ttgaataaac
     2101 aaccttgtaa ggtataatta aagtatcaaa aggagaaaca aaaatgaaac ttgcggcaca
     2161 gttatataca cttcgggaat acacccagac cgaaacggat tattacaaca catgtgttaa
     2221 ggtaaagaat atgggttatt cctccgtcca gctctccgga gcaggtccca tggaagcaaa
     2281 aacggttaaa agtatttcag atgagttaaa tctgcccgtc acggcaacac atattccaca
     2341 tacaatgttt gtaaatgaat tggataaggt aatacaaaac cataagataa tgggatgcaa
     2401 atatgtggga ttgggttaca tgcccaaaga gtattgggag agcgtaagta cattgaatga
     2461 gtttataacc acctattcga aggtttccga agcactggat aagcaaggct tacggttcgt
     2521 ataccacaat catcatttcg agttcttcaa gttcaagggc aagacaatga tggatataat
     2581 attggaaaat actccctcct gctttttctt tttgctggac acctactgga tacaggctgc
     2641 gggagcaaat ccggtatact ggataaagaa attaaaaggc agaggcgact atattcactt
     2701 caaggatatg ggtataggca gtgattataa aacaactttc gtgccggtag ggcaaggaaa
     2761 tctggactgg caaaacatta tacaagcatg cagatatgca gaagtaaaat atatagcagt
     2821 ggaacaggat acatgtgaag gttcgccgtt cgatgcgctg aaaacttcct atgattatct
     2881 gtcatcattg ggattgaagt aaggagattg atatgactga agtgcgattc gggattatag
     2941 gttacggaaa cattgcaggc acacatttga gtttttttga taaaaatgaa atacccgatg
     3001 cccgcgtaac ggccatattt gatacagata aatccagagc ggacaaggca agagaaaaat
     3061 ataagggtaa aatagcggtc tttgatgatg aagaagagtt tttctcatcc ggcctttttg
     3121 acgccatact catctgttct ccgcattatc atcatccgca ctatgccata aaaggattcg
     3181 agcacaacct tcatgtgctg tgcgaaaaac cggcgggagt gtatacaaaa caggtgcgtg
     3241 aaatgaatga ggcttaccgg catcataagc atcttgtttt tgccatgatg tacaaccaga
     3301 gaaccaaccc tctgtacaga aaggtccgtg atatgcttct aaacggcgaa tacggagaga
     3361 taaggcgctg tgtctggata attaccgact ggttccgtac tcaaagatat tacgattcgg
     3421 ggggctggag agctacctgg gcttatgaag gaggcggagt gctgttaaat caggatcccc
     3481 atcagctgga tttgtggcaa tggatatgcg gtatgcccgt aagagtacga gcattcttag
     3541 gtttcggaaa gtttcatgat attgaagtgg aagacgatgt aaccgcttat gtggaatatc
     3601 ccaacggagc aacgggagta ttcataactt ccacaggtga agcccccggc acaaacaggt
     3661 ttgaaatttc tttagacaga ggcaagatag taatagaaaa cgacaaaata tgcgcatata
     3721 aactgaagga agatctgagc ag
//
LOCUS       DAOPWC010000109         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24969_length_3736_cov_3.556099, whole genome shotgun sequence.
ACCESSION   DAOPWC010000109 DAOPWC010000000
VERSION     DAOPWC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24969_length_3736_cov_3.556099"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKJ65_03295"
     CDS             <1..433
                     /locus_tag="PKJ65_03295"
                     /inference="COORDINATES: protein motif:HMM:NF036563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5343 domain-containing protein"
                     /protein_id="HNZ40897.1"
                     /translation="PTERYKQFRNPSLSGKVLAKGLVDAYKPLFERNERFFDLSKEKF
                     NELVCEETGFDHDNITAKRITNTFFEVKRLADFSSVFDEETIEPEKDEAPAIQLQNNN
                     SQIQPVSKLGLSYNINLILPETDDPKVFNAIFKSLKENLLL"
     gene            430..1008
                     /locus_tag="PKJ65_03300"
     CDS             430..1008
                     /locus_tag="PKJ65_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HNZ40898.1"
                     /translation="MIANENKFRSFCFSCMAALCDLEKIQYVVNYPLIHKLESKQNSL
                     TDYFDQKIYYEAQNMAQYYTIFYCLERSVRDIVVEKLKAIHGGNWWETKVPETIQKNV
                     KENMQRELDAGVSIRSDQEIDYTTFGELGEIVKANWADFGDIFKSVKAFTKIMTSLNI
                     LRGPIAHCCSLSEDEVDRLNLVVRDWMRIQSK"
     gene            1284..1472
                     /locus_tag="PKJ65_03305"
     CDS             1284..1472
                     /locus_tag="PKJ65_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HNZ40899.1"
                     /translation="MFNRNSEKRFDIIHKEGTVSGFKIIVDRETGVNYLFSWDGYAGG
                     ITPLLDRDGKPVVTSNNV"
     gene            2021..2473
                     /locus_tag="PKJ65_03310"
     CDS             2021..2473
                     /locus_tag="PKJ65_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ40900.1"
                     /translation="MKVISIKKSPEYKDRAIKYFQSKWASPDTMKIYDDCITNCITTP
                     SPLPQWYLLMDNDRVIGCAGLITNDFISRMDLYPWVCAVYIEKEYRGNAYGSLLLEQA
                     KIDAKAGGFSHLYLCTDHIGYYEKYGFKYIGTGYHPWGESSRIYDAAL"
     gene            2952..3176
                     /locus_tag="PKJ65_03315"
     CDS             2952..3176
                     /locus_tag="PKJ65_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HNZ40901.1"
                     /translation="MKCPCCNQEMEEGYLQTGTRIAWTKKIHKVSLLPRKDEIMLENN
                     VFKGVNFQAYICKQCKKVLLDYSDKNYVEG"
BASE COUNT         1250 a          586 c          878 g         1022 t
ORIGIN      
        1 tccaactgaa cgttacaaac agtttagaaa tcctagctta tctggtaaag tgttggcaaa
       61 aggcttggtt gatgcataca aaccactatt tgaaagaaat gaaagatttt ttgatttatc
      121 aaaggaaaag tttaatgaac tagtttgtga agaaactgga ttcgatcatg ataacattac
      181 tgccaaacga ataacaaata ccttttttga agttaagaga ttagctgatt tttcatcagt
      241 gtttgatgaa gaaacaattg agccagaaaa agatgaagca ccagccatac aattgcaaaa
      301 caacaatagc caaatacagc cagttagtaa attgggtttg tcatacaata tcaatttaat
      361 attacccgag acggatgatc ctaaagtttt caatgcaatt tttaaaagtc taaaggagaa
      421 cttattgtta tgatagcaaa tgaaaataag tttcgttctt tctgctttag ttgtatggcc
      481 gccttatgtg atcttgagaa aattcagtat gtagttaatt atccattaat acataagctt
      541 gagtcaaaac aaaatagctt aacagattat ttcgatcaaa aaatatatta cgaagcacag
      601 aatatggcac aatattatac tatattttat tgtctcgaga gatcagtacg agacatagtt
      661 gtggaaaaat taaaagcaat tcatggaggg aattggtggg aaaccaaagt tccagaaact
      721 attcagaaaa atgttaaaga aaatatgcaa agggaactag acgctggagt tagtattagg
      781 tctgatcaag aaattgatta tactactttt ggagagctgg gtgagattgt taaagcaaat
      841 tgggctgatt ttggtgatat attcaaaagt gtaaaggcat ttacaaaaat tatgacaagc
      901 ttaaatattc tacgtggacc aattgctcat tgctgtagcc tatccgaaga tgaagtggat
      961 agacttaatc tagtggttcg agattggatg agaatacagt caaagtaaac gtaagaacga
     1021 gttaatgata ccttgatatt gatgcaaact gcgtataaca atgcttttac gctttgggcg
     1081 tgcttacggg tcattgctgc cacacagcct caccgggcgt caagcgccgc caggaagaaa
     1141 gttccgctgg tccggttcgt cggcgtcgta aaaccgggac gttagatgac acgtaatgca
     1201 gattgattat gtaataaagt ataagtttta tgttgaatat aataagaata atgataagta
     1261 taactaattc ttggaggtaa gaaatgttta atcgtaattc agaaaaaagg tttgatatca
     1321 ttcataaaga aggtactgta tctggcttta aaataatagt agacagagag accggggtta
     1381 attatttgtt tagttgggat ggatacgctg gaggaataac accattgttg gatagggacg
     1441 gaaagccagt tgttacatcg aataatgtat aatattgata taaggacaca aactcctaca
     1501 cggagtttgt cagacattta aacctgggat aggcaatatc tatattgtat accctattaa
     1561 ggtatagata ccgaaagtag tatagagata taaaggaaca aactcctatg cggagtttgt
     1621 aggtgattta aacccaagag aggtaatagt tacacaagcg acgcaagtca ggtcccggat
     1681 accggaagca gtatacaaag tttattagta aggcgttgag gggcattacg catcatctaa
     1741 caatgcactc acgcggcggc acggcagcaa aggcagggag tatgcgtgcc ttgggttgca
     1801 gaggatatgc aatactatag tttgacttag gtagcaacca caccccttca ccgggagcga
     1861 tgctccgcta tgaagtggaa ctctgtgggt ccggttcagt ggtgtcgtga gtgcgaaccc
     1921 gttatatgac agatggcgct aaggagtatt attcctatgt gtatataagt agaataaagc
     1981 gaagtaaaga atgtttgcat aacagaaagg tgtgaagtaa atgaaagtca tatctataaa
     2041 aaaatctcct gaatataaag atagagcaat taaatacttt caaagtaaat gggcatctcc
     2101 agatactatg aagatctatg atgactgcat aacaaattgc attactactc cgagtccatt
     2161 accgcaatgg tatctgttga tggataatga tagagtaatt ggttgcgcgg gtctcataac
     2221 aaatgatttt attagccgta tggatttata cccttgggtt tgtgctgtat acattgagaa
     2281 agaatacagg ggaaatgctt atggttccct tctgttagag caggcaaaga tagacgcaaa
     2341 agcaggaggc ttttctcact tatatctctg cacagaccat atcggatatt atgaaaagta
     2401 tggatttaag tatattggta caggctatca tccatgggga gaaagttctc gtatatatga
     2461 tgcagcatta taatatgttt cgcaaaaata ttgtaatgta aaaactccta tgggtcgttt
     2521 gttagagatt taaacccaag ggagacaata gatacataat cgacacatgg caggtcccgg
     2581 ataccggaag aggtatatga agattatagg gtagggccct gggggcgcca tccatcatct
     2641 aacaatgcgc tcacgcggcg gcacgggagg gaaagggcaa ggagcaatcc gtgccttggg
     2701 ctgccggata caattgcttt ggagtggggg cagggcaaca acaccccttc accgggagcg
     2761 agctccgcca aggggacagg ctctgtgggg ccggttcagg gacgtcgtga gtgcgaaacc
     2821 gttatatgac agatggcgca gattgattta tacaacaaat gttattttta tattacatat
     2881 cagtgaatta cgcgaggtag agtatattta tataccaaga agataagcag gaatttgaca
     2941 ggagggaaaa tatgaagtgc ccatgttgta atcaggaaat ggaagaaggt tatttgcaga
     3001 caggaacaag aatagcttgg acaaagaaaa tacataaagt ttctttactt ccaaggaaag
     3061 acgaaataat gcttgaaaac aatgtattta agggtgttaa ttttcaggca tatatttgta
     3121 agcagtgtaa aaaagtgttg cttgattatt ccgataaaaa ttatgtagaa ggctaaactt
     3181 taatttatca gcgagagtat gagcataagc gttaggtatt tatatatgat aaatctttac
     3241 ataaggtgca aactcctatg cggagtttgt taatattaaa acccgaggga gacaatattt
     3301 acataagcga cacatgtcag gtaccggata ccggaagcgt atagaaagat attaaggtag
     3361 agctctgggg acgccatcca tcatctaaca atgcactcac gctgcggcac gacgggctgc
     3421 ggcaaggagc aagcgtgcct tgggttgcaa agatatgcaa tactatagat tgctgtaagt
     3481 agcaaccaca ccccttcacc gggagcggag ctccgccagg gggatggact ctgaggtccg
     3541 gttcagtggt gtcgtgagtg cgaaaccgtt agacgacagg taatgcagat tgctgattgt
     3601 aaaaagataa atggatgatg aacttattaa gcagaaatat taatattgta ggtttgtacc
     3661 atattatggt agaattagat ggagactgtt gcaggtgtgg tatgcctcct tttactaagg
     3721 aggtgaggct atgagt
//
LOCUS       DAOPWC010000110         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_24977_length_3735_cov_4.101087, whole genome shotgun sequence.
ACCESSION   DAOPWC010000110 DAOPWC010000000
VERSION     DAOPWC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24977_length_3735_cov_4.101087"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="PKJ65_03320"
     CDS             <1..1516
                     /locus_tag="PKJ65_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40902.1"
                     /translation="AGTATITVTTEDGGKTATCTVKVVPAKPTGLVAIKPTTAGGTDG
                     MITGTSAIMEYANNVSFTGAVNCIGTKITNLSAGTYYVRVKATVAHDAGEATTVVVPA
                     FVAIEEEDEDIYIPVTASTIPANNGAVNIGYIESEGVVSLDLTDDKIEEIIKKSSKGN
                     VDFNVSGIENATSVEIPKEAFAAFGKADMSVTVKLPDGSITLDKEAVDSVSEQAEGDS
                     VNIELSVIEAKELNHLQEQSVKADDIVVDINISDGKGNLIKTFDGELTIQIPYKGTLP
                     VTVWYLNNAGQLEKIEATYKEGIVTFKLNHLSVYVLGQDKDRTKDWDNPFTDVNEKDW
                     YYDSVKFAHMNKLMYGVGYKQFDPHGSIPRGLLLTVLHRLEKTPEPKGYGAFKDVAAN
                     KYYSKAVNWAAENNIVTGYGDGNFGPNDLITREHLALILMNYAKYKGFDTSKKADLEI
                     FADSKSISIWAKEAMAWVVAEDIYVGDQANLNPAGNATRCQVAAIFKRFIENVE"
     gene            1898..2488
                     /locus_tag="PKJ65_03325"
     CDS             1898..2488
                     /locus_tag="PKJ65_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNZ40903.1"
                     /translation="MIALKEYFKENPVITNRDADNIGIGRHVLSDLASQGKIERIRPG
                     VYQKSGEILDDFVLISSNRDRVVFSHQSALYLHDLSDRVPNIFNISVPQGYNASHIKK
                     GYANLKIHYIKKDLYPIGISEIRTPLGNVVQVYDVERSICDIIVDREKIDAQIFVGAL
                     SAYFKGKNKDLRKLIKYGRQFGIEKEIRRYVAFFRD"
     gene            2752..2982
                     /locus_tag="PKJ65_03330"
     CDS             2752..2982
                     /locus_tag="PKJ65_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HNZ40904.1"
                     /translation="MIKQIESKELEFAIFCIENTAVHLGIKGSVLYKKLTEESDILYD
                     YIIPLYDALHTQSKEYIVDDILRVMEKRGIAA"
     gene            2979..3449
                     /locus_tag="PKJ65_03335"
     CDS             2979..3449
                     /locus_tag="PKJ65_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HNZ40905.1"
                     /translation="MKLYHASNVEVSKPDLVHSRKNLDFGVGFYTTPIYEQALSWANR
                     FRKTEGYAVISEYVFDKTAYQTLSILKFDAYSEDWLNFIISCRTGKDNTTYDIVIGGI
                     ANDKVFNTIELYLDNLIDRKEAIKRLKYERPNSQYCFRTEKALAYLSFERSNCL"
     gene            3446..3679
                     /locus_tag="PKJ65_03340"
     CDS             3446..3679
                     /locus_tag="PKJ65_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HNZ40906.1"
                     /translation="MKAHPILLQKKYARIVEQFAKKQGIEISTALDFFYGSMVYVLMS
                     EGISDFHCMSDTYIVDELIIEYSKALKKNQLDK"
BASE COUNT         1258 a          620 c          819 g         1038 t
ORIGIN      
        1 agcgggaacg gcgactatta ccgttacaac ggaagacggc ggtaaaacag ctacttgtac
       61 ggtaaaagta gttcctgcaa aacctacggg acttgttgca ataaaaccta ctacggcagg
      121 cggtaccgac ggtatgatta ccggcacaag tgcaattatg gaatatgcaa acaatgtttc
      181 ctttacaggt gccgttaatt gtatcggtac aaagattacg aacctttcgg caggaacata
      241 ttatgtaaga gtaaaggcaa ccgtagcaca cgatgccgga gaggcgacca cggttgtagt
      301 tcccgcattt gttgctattg aggaagagga cgaggacatt tatattcccg ttaccgcttc
      361 cactattccg gcaaataacg gtgcggtcaa cataggatat attgaatccg aaggtgtggt
      421 atcattggat ttaaccgacg ataagataga ggagattatt aagaagagca gtaaagggaa
      481 tgtggacttt aatgtttccg gaattgaaaa tgctacatcg gttgaaatac ccaaagaggc
      541 atttgcagca ttcggtaaag cggatatgtc tgtaactgta aaactgcccg acggaagcat
      601 tactcttgat aaagaagccg ttgattcggt ttccgaacag gctgaaggag attcggtaaa
      661 tattgaacta tcagtaattg aagctaaaga gttgaaccat cttcaggagc aatcggtaaa
      721 agcggacgat atagttgttg atatcaacat tagcgacgga aaaggcaatt tgattaagac
      781 ctttgacgga gaactgacaa tacagattcc ttacaaggga actttaccgg taacggtctg
      841 gtacttaaat aatgccggtc agttggagaa aatagaagca acttacaaag aaggaatcgt
      901 tacgttcaaa ctcaatcacc tttcagtata tgtactggga caagataaag acagaacaaa
      961 agattgggat aatcctttta ccgatgtaaa tgaaaaggat tggtactatg attcagtgaa
     1021 gttcgcacat atgaacaaac tgatgtacgg tgttggctac aagcagtttg acccccacgg
     1081 ttctatacct cgcggactgt tgcttaccgt actgcatcgt ttggagaaaa cgcccgaacc
     1141 gaaaggatat ggcgcattca aagatgtagc ggcaaacaaa tattacagta aagctgtcaa
     1201 ctgggctgcc gagaataata ttgtaaccgg ttacggagac ggaaactttg gccccaatga
     1261 ccttattaca agagaacatt tggcattaat ccttatgaac tatgccaagt acaaaggttt
     1321 tgacacatca aaaaaggcgg atttagaaat atttgccgac tcaaagagta tttccatttg
     1381 ggcaaaagag gctatggcat gggttgttgc cgaagatatc tatgtaggtg accaagccaa
     1441 tctgaatcct gccggtaacg ccacacgttg ccaagtagcg gcaatattca agcgtttcat
     1501 tgaaaacgta gagtaaagag gcagtatgca gttatgcatt aatacaattc aatagtttaa
     1561 caatcggtac catgctttcc ttgaaggtat ggtacctttt attgtgtacc tggcatggca
     1621 cagttctaac gggtgaaagt ccctttacag gtatctatcg ccaagtgtag tgaaccacaa
     1681 gctggaagaa gtaatgccgg ggtgaaagcc ggtcgtgtac actctgcgga tgaggttgac
     1741 ggaatactga ataaggagtt cggtatctga tggagaagta ttctgtaaac tactccgatg
     1801 taataaactc tacttattat ttctaagcaa ttcgacattt agcactttac agattcgctt
     1861 taattatgca taatctaaag cgaaagtgta ggaagatatg atagcgttaa aagagtactt
     1921 taaagaaaat cctgtcatta cgaataggga tgccgataat atcgggatag gaagacatgt
     1981 tctatccgat ttggcaagtc aaggaaaaat tgaaagaata agaccgggag tatatcaaaa
     2041 atccggtgag attttagatg attttgttct gatatcgtcc aatagagata gagttgtttt
     2101 ttcacaccaa tcagccctat atttacatga tttatctgat cgtgttccga acatctttaa
     2161 tatatccgta cctcaaggat acaatgcaag ccacatcaaa aagggatatg caaacctgaa
     2221 gattcactat attaaaaaag acttatatcc tatcggtata tctgaaatac gaacaccgct
     2281 cggaaatgta gttcaagttt atgatgtaga aagaagcatt tgcgacatca tagttgatag
     2341 ggaaaaaatc gatgcgcaga tttttgtcgg tgcgctttcc gcatatttca aaggaaaaaa
     2401 caaggattta agaaaactta taaaatacgg cagacagttc ggaatcgaaa aagagatacg
     2461 gcgttatgtg gcgttctttc gtgattaaca tagagagtat aaaagggaaa ataagaaact
     2521 tatattataa gaacggctac acacagagaa agtttgaata aacaaaaaga gattgaaaaa
     2581 atattacggg atatagaaaa cgatgacgtt ttgtcttata agataacgaa atccgccttg
     2641 cttaagcgtt gtgtcaatta tcttgatatt aacgtaacgt aatcgattgt tttaattgca
     2701 tattaagaca actttatatg tgttataatt atcctatcgt caggagggaa aatgattaag
     2761 cagatagaat caaaggaact ggagtttgct atattttgca tagaaaacac agctgtgcat
     2821 cttggcatta aagggagtgt tctgtacaag aagcttaccg aggaaagcga cattctttat
     2881 gattacataa taccgctgta tgatgctctt catactcaga gtaaagaata tatcgtggat
     2941 gatatcttga gggttatgga gaagagaggt attgcggcat gaaactatat cacgcttcaa
     3001 atgtagaggt ttcaaaacct gacttggttc attccagaaa gaaccttgac ttcggtgtcg
     3061 gtttctatac tacaccgatt tatgaacaag ctctaagttg ggcaaacagg tttcgaaaga
     3121 ctgaaggtta tgcagttatt tcagagtatg tctttgacaa aacagcttat caaactttaa
     3181 gtatacttaa atttgacgcc tattcggaag attggctgaa ttttataata agctgtcgta
     3241 caggaaaaga caacacaact tatgatatcg taattggcgg aatagcaaat gataaggttt
     3301 ttaacacaat tgagctttat cttgacaatc tgattgatag aaaagaagca attaagcgac
     3361 ttaagtacga aaggcctaat tcccaatatt gtttcaggac ggaaaaagca cttgcttatt
     3421 taagttttga aaggagcaac tgtttatgaa ggctcaccct attttgctac aaaagaaata
     3481 tgcaaggatt gtggaacagt ttgcaaaaaa acagggtata gaaattagca ctgctcttga
     3541 ttttttctac ggttccatgg tgtatgtttt gatgagcgaa ggaatatcgg attttcactg
     3601 catgtcggat acatatatag ttgatgaact tattattgag tattcaaaag ctttgaaaaa
     3661 gaatcaatta gataagtgat acgggaattt tccaaatttt ctctttaaca ggacttaatg
     3721 tatttgtatg acata
//
LOCUS       DAOPWC010000111         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25039_length_3729_cov_3.537561, whole genome shotgun sequence.
ACCESSION   DAOPWC010000111 DAOPWC010000000
VERSION     DAOPWC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25039_length_3729_cov_3.537561"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKJ65_03345"
     CDS             <1..682
                     /locus_tag="PKJ65_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNZ40907.1"
                     /translation="VNYINKPDAASTARVLYEYFGGASRLKISEELMDAVDKFDSAQY
                     TMEDIVNPKGWVMLSYLLDARTGLGRFRNFRVTNYDLMMMLLNYCPDHTIDEVMRLPD
                     VQERVKMYYEHEKLFRDQIKGCAEVVKDVVILHLKNCDPIYVGNRFYVYTLFPQATVS
                     IHEMWGLKKRNTVFTLGKSIINRGSDKDLAAICKKHGGGGLMNAATCQVPNGLADGIL
                     EDILQQLL"
     gene            696..1013
                     /locus_tag="PKJ65_03350"
     CDS             696..1013
                     /locus_tag="PKJ65_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNZ40908.1"
                     /translation="MERYAWRAKVKEGMLDEYIRRHDEIWPEMVKLLKKAGIKNYTIW
                     YNNNDLFGYYECEHGVEYAAKVQAESPIVDKWNEYMKDVMVMEMDPVTKAQPLMRKVF
                     FLE"
     gene            complement(1074..2261)
                     /locus_tag="PKJ65_03355"
     CDS             complement(1074..2261)
                     /locus_tag="PKJ65_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ40909.1"
                     /translation="MEFRICNHKYRKPTDWNIPKSLKDAGYENIFIGAWDVSRIPPEQ
                     YGFNVTHLSKQYNKYISEKYPNVQYTNSWFGEVNPVPYSDSRPKYYADRVCEQLERLK
                     SDDKPWFIWVDGVDPHLPCRPCKEFYDMYAPEDIPPFGGFGDTFQNKPYIQKQQILNW
                     RLENMSWEDWSETVRCYYAMITQIDHSFGIILDKLKECGMYDDTLVIYTSDHGDMCGS
                     HGMIDKHYIMYDDVLRIPMIMSYPKLFKNNSVDYRRCDTFVYNALDIPATICEICKIE
                     RNPDCHGRSLLSILKGEKPNKEQFPDYTVSASNGQQFGSFTQRSIRTDKYKYIWNLTD
                     IDEFYNLQTDPYELENLIYDISYTDIISDLRKLLKKELARCFDPFVKSGWLNRTLEEN
                     IKL"
     gene            2271..2429
                     /locus_tag="PKJ65_03360"
     CDS             2271..2429
                     /locus_tag="PKJ65_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40910.1"
                     /translation="MDSARIPFMNDCLAGAHTGVGQYALSNTIPSFANLSILGVRTAR
                     IFEQPTQS"
     gene            complement(2473..3582)
                     /locus_tag="PKJ65_03365"
     CDS             complement(2473..3582)
                     /locus_tag="PKJ65_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40911.1"
                     /translation="MAIKIGIVGMGGIGNTHARCYKADPLAQLVAVCDVIKEKADKAA
                     NEHNVQAFYCIEDMMKAHPELDLIDVTTSGFDNGSWHYEPVMKALDAGFNVMVEKPIS
                     NDNDEAREMVVTAQKKNLYLGCNLNHGFTGPADKAKELISQGKIGAPVYILHKVGFNG
                     SEYSYKPLPKDGPMRRFAGPYSHAYAFLSHPFSLMRSFGGDITHVQAFFDKPGGRKSA
                     NDLMWSINGINVKYQNGSIGYILSQRGDAIFGLGGWWSFEMAGTKGTFCIENCVEKLY
                     YWDGSKPASMGTMQAEVFDFGTTDFGITFNNRLHAFLEDMTNKVPPEHIRGSGRDALA
                     TLEYTVAAIKSFEEGGALVRPRALPPLHGTPGFVV"
BASE COUNT         1024 a          789 c          802 g         1114 t
ORIGIN      
        1 cgttaattac ataaataaac ctgatgctgc ctcaacggca agagtactgt atgagtattt
       61 cggaggagca agccgactta agatatccga agaactcatg gacgccgtag ataaattcga
      121 ttcggcacaa tataccatgg aagatatagt taatcctaaa ggttgggtaa tgctgtctta
      181 tcttctggat gcaaggacgg gcttgggcag attcagaaat ttcagagtaa ccaactacga
      241 tcttatgatg atgcttttaa actattgtcc cgaccacact atcgatgaag tcatgcgcct
      301 gcccgacgta caggaaagag taaagatgta ttatgaacat gaaaaacttt tccgtgacca
      361 gataaaaggt tgtgcggaag tggttaagga tgtggtaata cttcacttga agaactgcga
      421 tcccatatat gtaggcaaca gattttatgt atatactctg tttccgcaag ccacggtatc
      481 cattcatgag atgtggggcc tgaaaaagag gaataccgta ttcacactgg gaaaatccat
      541 tataaacaga ggttcggata aggatttggc tgctatatgt aagaaacatg gcgggggagg
      601 acttatgaat gccgcaacct gtcaggtacc caacggtttg gcggacggaa tactggaaga
      661 catactgcaa cagttgttat aaagcggagg actttatgga aagatacgca tggagagcta
      721 aagtaaaaga aggaatgctg gatgaatata tcagaaggca tgatgagatt tggccggaaa
      781 tggtaaagtt gctcaagaaa gcgggaataa agaattatac catctggtac aacaataacg
      841 atttattcgg atactatgag tgtgaacacg gtgtggaata tgccgcaaaa gttcaggcgg
      901 aaagtcccat agtggataaa tggaatgagt acatgaagga tgtcatggtt atggagatgg
      961 accccgttac caaagctcag ccgctgatga gaaaggtgtt tttcttagag tagttcaata
     1021 tataaaacat tgatatatat aaataaaaaa agataagaaa aataaaagct gttctacagc
     1081 tttatatttt cttccaatgt tctgttaagc caaccgcttt ttacaaaagg atcaaagcat
     1141 cgtgcaagtt ccttttttaa cagctttctt aaatcggaaa ttatatctgt ataactgatg
     1201 tcatatatca ggttttccag ttcgtaagga tcggtttgaa gattataaaa ctcatcaata
     1261 tcggtaagat tccagatgta cttatactta tcggtacgta tgcttctttg agtgaaactg
     1321 ccgaactgct gtccgttgga ggcggatacc gtgtaatcgg gaaattgttc tttattgggt
     1381 ttttctcctt taagaatact taataaggat cttccgtgac aatcgggatt ccgctctatt
     1441 ttgcatattt cgcatatagt agccggaata tccagtgcat tatatacaaa ggtgtcacat
     1501 cttctataat caactgagtt attcttaaaa agcttagggt aactcattat cataggtatt
     1561 ctcagcacat catcatacat gatatagtgc ttgtctatca tcccgtgaga tccgcacata
     1621 tcgccatggt cggatgtata tatcaccaaa gtgtcgtcat acataccgca ttctttgagt
     1681 ttatccagta ttattccgaa ggaatgatct atctgggtaa tcatggcata atagcatctg
     1741 acagtttccg accagtcttc ccatgacatg ttttccagtc tccagttgag tatctgctgc
     1801 ttttggatgt acggtttgtt ttggaaagta tcaccgaatc ctccgaaggg cggtatgtct
     1861 tccggagcat acatatcata aaactcttta caaggtctgc agggcaggtg agggtccact
     1921 ccgtccaccc atatgaacca gggtttatcg tcacttttta acctttccag ctgctcacat
     1981 accctgtcgg cataatactt aggtctgctg tcggaataag ggacgggatt tacctcgccg
     2041 aaccagctgt tggtgtattg cacattggga tatttttccg aaatatattt attgtattgc
     2101 tttgacagat gtgtcacgtt gaaaccgtat tgctcgggcg gtattcttga aacatcccat
     2161 gcgcctatga aaatattctc atatcccgca tctttaagac ttttgggtat gttccaatcg
     2221 gtcggtttac ggtatttgtg gttacaaatc cgaaattcca caatgctcct atggattcgg
     2281 ctcgcattcc gttcatgaac gactgtctgg cgggagcgca caccggtgtg ggacaatatg
     2341 cattgtcaaa tacaattcct tcttttgcca atctgtctat attgggagtt cggactgccc
     2401 ggatattcga gcagcctaca cagtcataac gatgctgatc tgccaaaaaa agcagaatat
     2461 tgggtttttt catcaaacta caaatcccgg tgttccgtgt aaaggaggaa gcgcacgggg
     2521 acgcacaagt gctccgcctt cttcaaagga ttttattgcg gcaacggtat attccaatgt
     2581 cgcaagagca tctcttcccg accccctaat atgctccgga ggaactttgt tggtcatatc
     2641 ctctaagaat gcatgcagtc tgttattgaa tgttataccg aaatcggtgg tgccgaaatc
     2701 gaatacttcg gcttgcattg tacccatgga tgcgggtttg gaaccgtccc agtaatacaa
     2761 tttctccaca cagttttcta tgcagaaagt tccttttgta ccggccattt caaaactcca
     2821 ccatccgcct aaaccgaata tggcgtctcc tctttgcgat aatatatatc ctatcgaacc
     2881 gttctgatat ttgacgttta ttccgtttat cgaccacata aggtcgtttg cactcttgcg
     2941 tccgcccggc ttatcgaaga aagcctgaac gtgagttata tctccgccga aacttctcat
     3001 caaagagaaa ggatgagaga gaaatgcata tgcatgagaa tatgggcctg cgaatcttct
     3061 catgggaccg tccttgggaa gaggcttgta tgagtactcg cttccgttaa atcccacttt
     3121 gtgaagtatg tataccggag cacctatttt tccctgcgag ataagctctt ttgccttatc
     3181 cgcgggaccg gtaaacccgt gatttaagtt gcagcctaaa taaaggtttt tcttctgtgc
     3241 ggtaactacc atttcacgtg cttcgtcatt gtcgttggat atgggttttt ccaccattac
     3301 gttgaagccc gcatccagag ccttcataac cggctcatag tgccaggaac cgttgtcaaa
     3361 gcccgatgtg gtaacatcaa tgagatccag ctcggggtga gctttcatca tgtcttcaat
     3421 gcaatagaaa gcctgcacat tatgctcatt agccgcttta tcggcttttt ccttaattac
     3481 atcgcacaca gcgaccagtt gtgccagcgg gtcggcttta tagcatcttg catgcgtatt
     3541 tcctattccg cccattccta ctatccctat cttaattgcc attttttctc tccttattcc
     3601 ttaatatttg ctacaataac cggtttattt gtttttaaag actgtatagc cgcatgtatt
     3661 atacggctgg cttccaggcc gtctttgccc gatccgtcta tatcttcagg tttgacacct
     3721 tccgcaact
//
LOCUS       DAOPWC010000112         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25201_length_3714_cov_4.276032, whole genome shotgun sequence.
ACCESSION   DAOPWC010000112 DAOPWC010000000
VERSION     DAOPWC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25201_length_3714_cov_4.276032"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKJ65_03370"
     CDS             <1..393
                     /locus_tag="PKJ65_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ40912.1"
                     /translation="THVKGLTTAAGLWTIAAVGLAIGAGFYEAAILCVIILAIVMAYL
                     GNFGSRRIKMDIYLEITGSRNINTVIKDIKKAGYTIDNIEVHSAKSGTYGNIGLSIVT
                     GIPKKSNEEKVIDDITAVENVVLVVRCD"
     gene            393..1319
                     /locus_tag="PKJ65_03375"
     CDS             393..1319
                     /locus_tag="PKJ65_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ40913.1"
                     /translation="MADKSNLNGIPVFRIEDNPDGHLMFYVKKYRPENGTHYLHAHEC
                     IQINYVYGGKAVHIIHNKKYDLVKGDIFVIPPNVPHQIQPAGVSDTEIVEFEFDPKFI
                     NEFLNDLKKGESFWDFAYIEPFLVEEHSVRPRFNLVGKIQCETENIFNEILREYENRE
                     PGFDLIIRSLLLKLLVMAGREFTKSISNSKSAKLFERHSDAIKGAIRYIEENYTLDLN
                     IDTVAKKFHLSPSFFGYLFKSLTSKTFKEYITGLRIAKASEMLKHTDDLVINICYESG
                     FNNVNHFNRTFKQIIGMTPGQYRKRFMNGNSQ"
     gene            1407..2762
                     /locus_tag="PKJ65_03380"
     CDS             1407..2762
                     /locus_tag="PKJ65_03380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ40914.1"
                     /translation="MSELAIFGGKPAIDKTKVPDSLFKWPIVTREDEDAVLDVLRRGA
                     MSGLDVTEKFEKEFAKWQKRKYAIGYCNGTMSLQAAMYACKIGAGDEIICPSKTYWAS
                     CLQAFNLGATVVFADIHPRTLCIDPDDIERCIGPRTKAIMVVHYLSHPADMDPIMAIA
                     KKHNIYVIEDVSHAQGGMYKGRMLGTFGHVAAMSAMSGKSFATGEMGILVTDDKEMYD
                     RAMAYCHYERNNSKYISTDYLLKYASMPLGGVKGRVNQMCAAIGRVQLKYYDERTAEI
                     RKAMKYFWSLLEGVPGIRAHMVDESDGSNMAGWYAPHGLYCPEELEGLSVERFCEAVQ
                     AEGFAARAGGNRALHLHDIFKDYNGLNYDKPARVAFTDRDVRKLDEGITVSQHVQIYS
                     IPWFKHYMPECIEMYANAFKKVALNYKELLAGDEKRGTESGRWFFYTDKKEKKEKKGV
                     K"
     gene            2759..>3714
                     /locus_tag="PKJ65_03385"
     CDS             2759..>3714
                     /locus_tag="PKJ65_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ40915.1"
                     /translation="MSKRIIRYGIIGCGGIAHTHAEALLQIKEAKLVGVSGCSPQEPI
                     PFAEKYGIKSFDSNEHLLLSDEIDAVTICTPSGYHAPIAISAAQHKKHAIIEKPLALT
                     TESAKQVVQAFAKNGVTGSVISQLRFYDGIRKVKSIIEDKRLGDLIMANLTMKFHRSQ
                     EYYDSGKWRGTWELDGGGAMMNQGIHGVDALLYFIGEVKNVYGYIRTLTHKIEVEDNA
                     VAVVEFRNGCLGTIIASTSMVPACNRTIEIHGTKGTVLLEEEHIARLYIEGEKEYSAD
                     DIAQGEAASSHIITAGGHVKQLTDFTMALVEGRAPYVSLEDG"
BASE COUNT         1239 a          651 c          924 g          900 t
ORIGIN      
        1 actcatgtaa agggactgac caccgctgcg gggctttgga caattgccgc tgtaggttta
       61 gcaataggag cgggatttta tgaagctgct attctgtgcg ttataatact tgcaatagta
      121 atggcttact taggcaactt cgggtccaga aggataaaga tggatattta tctggagatt
      181 accggcagca ggaacataaa taccgttata aaggacatta aaaaagcggg atacaccata
      241 gacaatatcg aagtgcatag cgcaaaaagc ggcacatacg gcaatatagg attaagcatt
      301 gtaacaggca taccgaaaaa atccaatgaa gaaaaggtaa tagatgatat cacggcagtg
      361 gaaaatgtag tacttgttgt aagatgtgac tgatggcgga taaaagcaat ctaaacggca
      421 taccggtttt cagaatagaa gataatcctg acggacatct gatgttttat gtcaaaaaat
      481 accgaccgga aaacggaaca cattatctgc atgctcatga atgcatacag ataaactatg
      541 tatacggcgg aaaagccgtt catatcatac ataacaagaa atatgacctt gtcaaaggag
      601 atatttttgt cattcctccg aatgttccgc atcagataca acccgcggga gtaagcgata
      661 ccgaaatagt ggagtttgaa tttgacccca aatttattaa cgaatttctt aatgatttga
      721 aaaagggcga atcgttctgg gattttgcat atatagaacc ctttttggtg gaagagcatt
      781 cggtcaggcc gaggtttaat ctggtaggca agatacagtg tgaaacggaa aatattttta
      841 acgaaatact cagggaatat gaaaacagag agccgggatt tgaccttata atccgctcgt
      901 tgcttttgaa gcttttagta atggcgggaa gggagtttac caaaagcatc agcaacagca
      961 aatcggctaa actatttgaa agacacagcg acgcaataaa aggagcaatc cgatatattg
     1021 aagagaacta tacgctggat ttgaatatag ataccgtggc taagaagttt catttgtctc
     1081 cctctttctt cggttatctg tttaaaagcc ttacatccaa aacattcaag gaatatataa
     1141 ccggattgag aatagcaaaa gcgtcggaga tgctgaagca caccgatgac ctggttataa
     1201 acatatgcta tgagtcggga tttaacaatg taaaccattt taaccgcacc ttcaaacaga
     1261 ttatcggcat gacaccggga caatacagaa aacgatttat gaatggaaac agtcaataaa
     1321 taagtggaaa gtgcaaagaa acaccgcaat aaagagatta atgtataata gagaaagtgt
     1381 atttatacaa gaggaggaaa atataaatga gtgaacttgc aatattcgga ggaaagcccg
     1441 caatagacaa aacaaaggta ccggattcgc tttttaagtg gcctatagtg accagggaag
     1501 atgaagatgc cgttttggat gtcttaagaa gaggcgcaat gagcggactg gatgtaacgg
     1561 agaaattcga gaaagagttt gccaagtggc aaaagagaaa atatgccatc ggatattgta
     1621 acggaacaat gtcacttcag gctgcaatgt acgcatgtaa aataggagcg ggcgatgaaa
     1681 taatatgccc cagcaagacc tattgggcat cctgtcttca ggcgtttaat ctcggtgcaa
     1741 cagtggtttt tgcagatata catcccagga cattatgtat cgatcccgat gatatagagc
     1801 gatgcatagg accgagaacc aaagcaataa tggttgtgca ttatctttca catccggccg
     1861 acatggatcc cattatggcc attgcaaaga aacataacat ttatgtgata gaggatgtat
     1921 cccatgctca gggcggtatg tataaaggca gaatgttggg tacattcggt catgtggccg
     1981 caatgtccgc tatgagcgga aaatcttttg ctacagggga aatgggtatt cttgtaaccg
     2041 acgataagga aatgtatgac cgtgctatgg catattgcca ttatgaaaga aacaattcca
     2101 agtacatatc caccgattat cttcttaaat atgcttccat gcctttgggc ggagttaaag
     2161 ggagagtaaa ccagatgtgt gccgcaatcg gaagggtgca actgaaatat tatgatgaga
     2221 gaactgccga gataaggaaa gctatgaaat atttctggag tcttctggag ggagttccgg
     2281 gaatcagagc acatatggtg gatgagtcgg acggatccaa tatggcgggg tggtatgctc
     2341 cccacggatt atactgtccg gaagaactgg aaggactttc tgtagagaga ttctgtgaag
     2401 cggtacaggc ggaaggcttt gcagccagag cgggcggaaa cagggctttg catctgcatg
     2461 atatatttaa agactacaac ggattgaatt acgataagcc tgcaagagtg gcatttaccg
     2521 acagggatgt gcgtaagctg gatgaaggta taacggtatc gcaacatgtg cagatttact
     2581 ccataccgtg gtttaaacac tatatgccgg aatgcataga gatgtacgca aacgctttta
     2641 agaaggtggc tttgaattac aaggagttgc ttgccggaga tgaaaagaga ggaacggaaa
     2701 gcggaagatg gttcttctat accgataaaa aggaaaagaa agagaaaaaa ggagtaaaat
     2761 gagtaaaaga attatcaggt acggaataat aggatgcggg ggtatagcac atactcatgc
     2821 cgaggctctt ttacagataa aagaagcaaa acttgtggga gtcagcggtt gttcaccgca
     2881 ggagcctatt ccttttgccg aaaaatacgg cattaaaagt tttgacagta atgagcatct
     2941 tctgttaagc gacgagattg atgccgtaac aatatgtact ccgagcggat atcatgcacc
     3001 tatagccata agtgcggcac agcataaaaa acacgctatt attgaaaaac cgcttgcctt
     3061 gaccacggaa agtgctaagc aggtagtaca agcttttgcg aaaaacggcg tgaccggcag
     3121 tgtaatttca cagttaaggt tttatgacgg aataaggaaa gtaaaaagca taatcgagga
     3181 caaaagactc ggtgatctta ttatggccaa ccttaccatg aaatttcaca gatcgcagga
     3241 atactatgat tccggtaaat ggagaggcac ctgggaactg gacggcggcg gagcaatgat
     3301 gaatcagggt attcacggag tggatgcgct tctttatttt ataggagaag taaagaatgt
     3361 ttacggctac atacgcactt tgacacacaa aatagaggtg gaagataacg cggttgcggt
     3421 tgtggaattc aggaacggtt gcttgggaac aataattgcg tccacatcaa tggtaccggc
     3481 atgcaacaga acaatagaga ttcacggcac caagggtaca gtacttctcg aagaggagca
     3541 tattgcacgt ctttacatag aaggcgagaa agagtattcg gctgatgata tagcacaggg
     3601 agaagcggca agcagccata ttattacagc cggcggacat gtaaaacagc ttaccgattt
     3661 taccatggct cttgttgagg gaagagcacc ttatgtttca ctggaagacg gaat
//
LOCUS       DAOPWC010000113         3707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25261_length_3707_cov_3.525739, whole genome shotgun sequence.
ACCESSION   DAOPWC010000113 DAOPWC010000000
VERSION     DAOPWC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25261_length_3707_cov_3.525739"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PKJ65_03390"
     CDS             <1..1415
                     /locus_tag="PKJ65_03390"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNZ40916.1"
                     /translation="NIVSVKSVKKTKRKKYPFPPFITSTLQQDASNKLGFTTSKTMKI
                     AQELYEGVELKNEGSVGLITYMRTDSTRISADFQMATRDFIKQNFGSEFLPSNIPSYK
                     NRNNTQDAHEAIRPTDFNLTPTRVSASLTKDQASLYTLIYNRYIASQMAPAEYDNTAV
                     DIVCGPYLFKAVGNIVTFKGYMTVYEEHRIEQSDSEKDEYENTYLPKLSAKEQLMKHD
                     LQKIQKFTTPPSRYTEASLVKMMEEKGIGRPSTYAPTINIILKRNYVKREKRILVPTD
                     LGMTVNSFMVKNFETLLDYGFTAQMEEKLDNIETGKVDWISLIDKFYKDYIEQIYIAD
                     LRTDKIDLPSDEVCEKCGKPMLIKSGRYGDFLGCSGYPACNFTKKIIDDTGYKCPKCN
                     GKVLNKKTKSGVKFISCENYPKCDFSSWGTIIKDRKCPKCDSFLLKVFEDKQAMTKCS
                     SDKCDYKTKFVSVTKRKKEK"
     gene            complement(1419..2789)
                     /locus_tag="PKJ65_03395"
     CDS             complement(1419..2789)
                     /locus_tag="PKJ65_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HNZ40917.1"
                     /translation="MKLKVVLSVLSCKITCAVLRAFKKGGTSLPGKIALLFCKDILKY
                     VSKNVKVILVTGTNGKTTTCRIIETALEKAGINYFSNKSGANLLNGVVTSFCLASSLS
                     GKNRHDYAVIECDEAALRQVTKYINPQVILVTNIFADQLDRYGDVFNILRLIADGINN
                     CDSATICINADCSLCTHLMTLTEKEARLFGVDIEIPDTHINEQNGILHCIKCGNPYTY
                     DYVTYAHLGGFRCDNCGYRRHKPDVGAVSILNMDITGSDIIMSMNNRQYNLRVNLAGA
                     HNIYNALAAACALNTVGMEDKHILTAVSTFKSGFGRMEIIEINNIPVNIILVKNTAGF
                     NQIINYLFLVENEYILSVILNDKPADGTDVSWINDVDFEKLNETSKKIKYVYLGGTRA
                     GDMALRLKNAGFDMNKVYTVKNDKDLVNKALEHNIPIYITATYTAMFNLRNMLTGKYS
                     LKEFYK"
     gene            2959..>3707
                     /locus_tag="PKJ65_03400"
     CDS             2959..>3707
                     /locus_tag="PKJ65_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNZ40918.1"
                     /translation="MENKLKRAVKSLPIIFVLVFAISGCGKVCIFSSEETSHPPNFYT
                     LPIQTEDSTEAVTSPNVTENIPNSDSPTMTPEAKIPADDDLVRVLDYIPTIYIDLKYA
                     TTDNFTGTVIYDFKDAYLRYGTVKKLFTVQEELSEQGYSLKIWDAYRPVSAQYKLWQV
                     YPDPTYVANPNTGYSSHSKGNTVDVTLVFLDGSDVKMPSAFDDFSSYADRDYSDVSND
                     ASENASLLEKIMERNGFSGYSGEWWHYSDTT"
BASE COUNT         1207 a          711 c          725 g         1064 t
ORIGIN      
        1 ataacattgt ttcggtaaag tcggtaaaga agaccaagag aaagaaatat cctttcccgc
       61 cttttataac ctctacatta cagcaggatg cttccaataa attgggtttc acaacatcca
      121 aaaccatgaa aattgcccag gaactttacg aaggagtgga gttgaagaat gaaggatctg
      181 taggacttat tacctacatg agaaccgatt ccacacgaat atccgccgat tttcaaatgg
      241 ctacacggga ttttataaaa cagaatttcg gcagtgaatt cctgccttca aatataccca
      301 gttataaaaa ccgcaataat actcaggatg cacatgaagc catacgtcct acggatttca
      361 accttacacc taccagagta tcggcaagtc tcacaaaaga ccaggcttca ttgtacacat
      421 tgatatacaa caggtatatt gccagtcaga tggcacctgc ggaatatgat aatacagccg
      481 ttgatattgt ttgcggacct tatctgttta aagccgtcgg taatattgtg acctttaaag
      541 gctatatgac cgtttacgaa gagcatagga tagaacagag cgattcggaa aaagacgaat
      601 atgaaaatac atatcttccg aaactttctg caaaagagca gcttatgaaa cacgatttac
      661 agaaaataca gaaatttacc acccctcctt caaggtatac cgaagcatcg ctggtcaaga
      721 tgatggagga aaaggggata ggaagaccca gtacttatgc tcctactatt aatattattc
      781 tgaaaagaaa ctatgttaaa agagaaaaga ggatactggt acctaccgat ctgggtatga
      841 ccgtaaattc attcatggtt aagaatttcg aaactctgct ggattacgga tttaccgctc
      901 agatggaaga gaagttggat aacatcgaaa cgggaaaggt tgactggata tctttaatcg
      961 ataaattcta caaagattat atcgaacaga tttatatcgc ggatttacgg acggataaga
     1021 tagatttgcc gtccgatgaa gtatgtgaaa aatgcggaaa acccatgctt ataaaatccg
     1081 gaagatacgg agattttctg ggatgctccg gttatccggc atgtaatttt accaagaaaa
     1141 taattgatga tacgggttat aaatgtccta aatgcaacgg taaggtattg aacaagaaga
     1201 ctaaaagcgg cgttaagttt atttcctgcg aaaattatcc caaatgcgat ttttcatcat
     1261 ggggaaccat tataaaagac agaaaatgtc ctaaatgcga tagtttcctt cttaaggtat
     1321 ttgaagacaa gcaggctatg acaaaatgtt cctcggataa atgcgactat aaaaccaagt
     1381 ttgtcagtgt cacaaagaga aaaaaggaaa aatagatact atttgtagaa ttcctttaac
     1441 gaatacttcc cggtcagcat atttctcaga ttgaacatag cggtatatgt ggcggttata
     1501 tatatcggta tgttgtgctc taaagcctta tttacaaggt ccttatcatt ttttaccgta
     1561 tataccttat tcatatcgaa accggcattt ttaagtctta aagccatatc cccggctctt
     1621 gttcctccca gataaacata ttttattttc ttgcttgttt cattcaactt ctcaaaatca
     1681 acatcattaa tccaggagac atcggtaccg tcggccggtt tgtcattcag tataacggac
     1741 agaatatatt cgttttcaac taaaaacagg taattgataa tctgattaaa gccggcagta
     1801 ttttttacaa gaattatgtt aaccggtata ttatttattt ctatgatttc catgcgtccg
     1861 aaaccgctct taaaagtgct gaccgctgtt aaaatatgtt tgtcttccat gcccacggta
     1921 ttcaaagcgc aagccgccgc cagtgcgtta tatatattgt gagcacccgc aagattaacg
     1981 cgaagattgt attgtctatt attcatggac ataataatgt cactgccggt tatatccata
     2041 ttcaatatgg aaacggcacc gacatcgggc ttatgacgcc tataaccgca gttatcgcat
     2101 cggaaaccgc ctaaatgagc atatgtgaca taatcataag tatacgggtt accgcactta
     2161 atacagtgta aaataccgtt ctgttcatta atatgagtat cgggtatttc tatatccaca
     2221 ccgaataatc ttgcttcttt ttcggttaaa gtcatcaaat gcgtgcataa tgaacaatcg
     2281 gcattaatgc atattgtcgc ggaatcacaa ttattgatac cgtcagctat caggcgtaat
     2341 atattaaata catcaccgta tctgtccaac tgatcggcaa atatattggt taccagtata
     2401 acttgcggat ttatatattt ggtaacctgt ctcaaggctg cttcatcaca ctctattacc
     2461 gcgtaatcat gacggttttt ccctgataat gaagaagcca gacaaaaaga ggtaacaact
     2521 ccgttaagca gattagctcc cgatttgttg gagaaataat taattcccgc tttctccagg
     2581 gcggtctcta tgattctgca ggtggttgtt tttccgttgg taccggttac cagaataacc
     2641 ttaacattct ttgaaacata tttaagaata tccttacaga acagcagtgc tattttgccg
     2701 ggaagggaag ttcctccttt tttaaaagct ctgagaacgg cacaggttat tttacatgat
     2761 aataccgata aaaccacttt caatttcata aaaaaattat atcagtgcaa aagaggatta
     2821 tcaataaata aagaaagatg aatatgggaa attattgaca aaggtcaaat tttgagatat
     2881 gttgagccta gaaatctacc aagcaaaaga ggaatgttat ttgtatatat attattttga
     2941 gtgaataagg tgctgaaaat ggaaaataaa ctaaagcgag cagttaaatc tttgccaatt
     3001 atatttgtgc tggtatttgc tatttcagga tgcgggaaag tttgtatttt tagcagtgaa
     3061 gaaacgagcc atccgcctaa tttttacact cttcccattc agacagaaga ctccacagaa
     3121 gcagtcactt caccaaatgt gacagagaat attccaaaca gtgattcacc gacaatgaca
     3181 ccggaagcga agattccggc tgatgatgat ctggtcaggg tgcttgatta cattccaacc
     3241 atatacattg atctgaaata cgcaacgaca gacaatttca cgggaactgt tatatatgat
     3301 ttcaaggatg catacttgcg ctatggtaca gtcaagaagc tctttaccgt gcaggaagaa
     3361 ttatcagaac aggggtacag cctcaaaata tgggatgcct acagaccggt aagtgcacaa
     3421 tacaagctct ggcaggtata cccggaccct acatatgtag ctaatccaaa caccggctat
     3481 tcatctcaca gtaaagggaa cacggtagat gtgacgctgg ttttcctcga tggttcggat
     3541 gttaaaatgc cgtcagcgtt tgatgatttc tcatcttatg ccgacagaga ttatagtgat
     3601 gtttccaatg acgcatccga gaatgcgtct ctacttgaga agattatgga acgtaacggt
     3661 ttttccggat actccggtga atggtggcat tattcagaca caacaga
//
LOCUS       DAOPWC010000114         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25465_length_3684_cov_4.400661, whole genome shotgun sequence.
ACCESSION   DAOPWC010000114 DAOPWC010000000
VERSION     DAOPWC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25465_length_3684_cov_4.400661"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..971)
                     /locus_tag="PKJ65_03405"
     CDS             complement(108..971)
                     /locus_tag="PKJ65_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ40919.1"
                     /translation="MCISFDDLPVVSYGITDTLYQKELIFKLINSLTSKKIPAIGFVN
                     EMKLYKKDSLNHFQIRLIENWVNNNLDLGNHTYSHPDYNSVTYDEYTRDIMKGGIISS
                     KLLKTHGRELMYFRHPFLHVGNTKSKADSLEEFLKNNGYTVAPVTVDNEDYLFALAYK
                     RALAKNDIELAKRIGTDYISYMEEKIIYYEQQTIELFGRNICQILLVHASALNSDYIG
                     SLINMLGNRQYTFVSISEALKDPAYLTQVSVYGNWGISWIDKWAFSQGKKGDFFRNDP
                     KTPDYIRELAE"
     gene            complement(2149..2565)
                     /locus_tag="PKJ65_03410"
     CDS             complement(2149..2565)
                     /locus_tag="PKJ65_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNZ40920.1"
                     /translation="MNGCTISSSKNEDIKTTIIELEKQALAQWNNGNPDGFLDLCADD
                     VVYIDPAFESKLEGKKALEDYYDTIRGKIKIDLYKMIDPVVQVTAEAAVLTYNYETQR
                     DGMVFKMNCTEVYRLDPTNKWKIIHTHWSFVQPGKI"
     gene            2656..2856
                     /locus_tag="PKJ65_03415"
     CDS             2656..2856
                     /locus_tag="PKJ65_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40921.1"
                     /translation="MIIGQENLTECAGKPGVPRKALKGVFQNAVIHSVGFSGISLAQQ
                     SGFAWLLPQIFLYLYQIALMSL"
     gene            3216..>3684
                     /locus_tag="PKJ65_03420"
     CDS             3216..>3684
                     /locus_tag="PKJ65_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40922.1"
                     /translation="MQTDLPSGKYTLRAYTAWMQNFPEIYMFHKEITLVNPLDAKSDS
                     LTVQASKDDSLFGIQFLPESGRYIPGDLAVFAFKAVTTDGRGIGVTGAVFNSRDSIIT
                     SFSDTYKGMGKLIFHPAPNERYYAVIRSKQGTELKQSLPALSETGAVIHVTKRG"
BASE COUNT         1029 a          718 c          798 g         1139 t
ORIGIN      
        1 caaattagag ataagacagc ataaacgggc ccgctgctat aagaaagatt tgtagttatt
       61 ataaatcgga aatcgggtga ccggaaatat ttagtcatat agggttgtta ttcggccaac
      121 tcccggatgt agtcaggtgt tttgggatca ttcctgaaaa agtcaccttt tttaccttgc
      181 gagaaggccc atttgtcaat ccatgatatg ccccagtttc cgtaaactga cacctgggta
      241 agataggctg ggtctttcag tgcctctgaa atactcacaa aagtatattg gcggttaccc
      301 agcatattga taagggaccc gatataatcc gagttcagag cactggcatg taccagaaga
      361 atctggcata tgttccggcc aaacagctca atggtctgtt gttcgtaata gatgattttt
      421 tcctccatgt aagagatata gtccgtacct attcttttgg caagttcaat atcgtttttt
      481 gccagggcac gtttataggc aagagcaaac agataatctt cattatctac cgtaaccggg
      541 gcgaccgtgt atccattgtt tttcagaaac tcttcaagcg aatcggcttt gctctttgtg
      601 tttcccacat gaaggaaggg atgtctgaaa tacataagct ccctgccgtg tgtctttaat
      661 aatttgctgg aaattattcc ccctttcatt atgtcccggg tgtattcatc ataagttaca
      721 gaattatagt caggatgtga ataggtatga ttgcccaggt ccagattgtt attaacccaa
      781 ttttcaatga gacggatttg gaaatgattc agactgtctt tcttatacag tttcatttca
      841 ttcacaaaac ctatggctgg aatttttttg gaagtcagtg agttaatgag tttaaagatg
      901 agttctttct ggtaaagtgt gtccgtgatc ccataactga caaccggaag atcatcaaat
      961 gagatgcata ttttcttttg ctgtccgaaa gaaagagcaa aagcaggaag aaataaacaa
     1021 aggaaaagta ttcgtttcac aggcaatgga aatttaattc attctattcc caaatgtaac
     1081 gatctcttaa tgattttgaa aatatgtttt aataacaatg cctgatcttt atctttgact
     1141 ttaggtatgg ataaagacag aacagctgta ttgtgcaatt cttttgtcgt attgtttttt
     1201 tgaaaggtta aatgttaatg ttaatggcgg gattgtcggt tgcacaacga caatcccgct
     1261 cgcgaataag agagggggca gtccgcatcc cttccgccgt tccgtttgtt ttgataagca
     1321 gtattacaca cggaattaca tctcggacgg taccattcag aaagtgatcc ccacaaaata
     1381 accggatgtc ctgttatgtt tcccgtgaag gaacaatgag aggactccgt ttcagatcat
     1441 ataaccagaa gataaagatt agcataagcg attgagaggc agcataaatt atatcctccc
     1501 atcctgcctg gccgtcccat acgatcctgg aagttaaaaa aagtccatag catgcaaaga
     1561 aacccatttg gtatctcaaa gattttttca taaccatatg cttttggaat tccggttaaa
     1621 gttataatat attttgatat gttgtaactt tttgacagaa agaggtgtat gcatatttat
     1681 tttatgtact tttgcgttgg caccggtcac tgaatgaatc tataaccata aatgatgaat
     1741 actatgaata gaaaaataat catcaccaat gcggttattt ttatttgtgt ctttttatta
     1801 tactactttt tcatttaccg gagtttttgg gaagcatttg taagtagttt gattctggtg
     1861 ggtatgattt tctggagcac gtatactgcc aggaagaggc tggataggtg gcccggtgga
     1921 aatacccggt agggtactcg ccagggtgct ctcccgtctt tttcgttcaa gaacttccga
     1981 caagatattc agaaaaaggg aaacctcgcg cccgcggagg gaaggaccca ttggcacagc
     2041 caatgggagg cgtttcccgt tctgaatatc ttgtggtgga gttctatgtg gtgcggccgg
     2101 tttgtggaac cggaagttgc gcagccggaa ggagtcttgg aatcctatct atatctttcc
     2161 gggctgaaca aaagaccaat gtgtgtgtat gattttccac ttatttgttg ggtctaacct
     2221 gtatacctct gtacaattca ttttgaaaac catgccatcc cgttgggttt catagttgta
     2281 ggtgaggaca gcagcctcgg cagttacctg taccaccggg tcaatcattt tgtacagatc
     2341 aatcttgatt tttcccctaa tggtgtcata gtaatcttca agcgcttttt tcccttcaag
     2401 tttactctca aaagcagggt cgatgtaaac aacgtcatcg gcacacaaat caaggaatcc
     2461 atcgggattc ccgttgttcc attgtgccaa tgcctgtttt tccaattcaa tgatggtagt
     2521 cttaatgtcc tcattttttg aggatgatat ggtgcatccg ttcatcgata ataaaattag
     2581 aatgaataaa atgtgatttc tcatgtctga taaatatgag gtaggttaat ttgagaataa
     2641 atttaagagt tttttatgat tattgggcag gaaaacctta cagaatgcgc gggtaaaccc
     2701 ggagtgccgc gcaaagccct taaaggtgtt tttcaaaacg ctgttattca ttctgttgga
     2761 ttttcaggaa tttctcttgc gcagcaatcc gggtttgcct ggcttttgcc tcagattttt
     2821 ttgtatcttt atcaaatagc gcttatgagt ctgtaagatt ggtaccagaa taaccagcct
     2881 atacaacaat gcgattcgtc tttctgtctt tatttgtact cacaatctcc ggcatccatt
     2941 gcagtgcgca ggatattacc gggattgagc cggctctgta tagttttgag tgcttttcga
     3001 cacttctatc tcccgagaag gtgtatctgc acacagacaa acaggtctat tgtacaggag
     3061 aatatatctg gtttaatgct tatttaacga acaactcatg tttctctttg ttgccggaaa
     3121 gcaactacat ctatgcagaa ctcatccaca aggataccgt actgtcccgg gtaaagatta
     3181 aacggtgcgt tgagggtttc ccggggagaa tccctttgca gacagatttg ccatcgggaa
     3241 aatatacttt acgggcatat acagcctgga tgcaaaactt tcccgaaata tatatgtttc
     3301 acaaagaaat tacccttgtc aaccctttgg atgcaaaaag cgatagtctc accgtgcagg
     3361 catctaaaga tgattcattg tttggcattc aatttttgcc ggaaagcgga cggtatattc
     3421 ccggggacct tgccgtgttt gcgtttaaag cggttaccac cgacggcaga gggattggag
     3481 taacaggtgc cgtctttaat agcagagatt ctataattac atctttttct gatacttata
     3541 aaggcatggg gaaattaata ttccatcctg cacccaatga gcgatactat gccgttatca
     3601 gaagcaaaca gggaacagag ctaaaacaat cgctaccggc tctttcggaa acaggtgcgg
     3661 taatacatgt gacgaaacga ggcg
//
LOCUS       DAOPWC010000115         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25481_length_3683_cov_3.959206, whole genome shotgun sequence.
ACCESSION   DAOPWC010000115 DAOPWC010000000
VERSION     DAOPWC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25481_length_3683_cov_3.959206"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKJ65_03425"
     CDS             <1..837
                     /locus_tag="PKJ65_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNZ40923.1"
                     /translation="HGVLDMFRNPKDAAYVYTSQTDKYPVMHVATSMSKGDYPMAMTK
                     SVWAFTNCDYIKMSINGKHIKDFYPDRKRFKHLLHPPIEIDDFTGNMLIDDLHFSEYS
                     SRVIKKLLSHIYVNGPENLPLGYMLMLVTVLIKEKLTINKLTEIITAHVAGWGDNVKQ
                     YVFEGIKDGKTVITEIKEPMNKMQLVLSADAHELKEEETYDTASVTIRAVDQNMNLLN
                     YCNEPLRIVTEGPIKLIGPELISLKGGCAGVYVKTDKQSGKAVLRVIGKDVTAEKEFS
                     VS"
     gene            complement(839..2089)
                     /locus_tag="PKJ65_03430"
     CDS             complement(839..2089)
                     /locus_tag="PKJ65_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ40924.1"
                     /translation="MNWIDRTITNHLLHAMDFFPAVLLTGPRQVGKSSLLQMVAKDFE
                     YITFDNALILQQAKSDPELFLMSHSQKLILDEIQYVPELFSSLKIRIDNMRFEALSNN
                     KPQECLYLLSGSQAYELMQNVSESLAGRLAILPLQGISYREQKKISCALPFLPTEQYL
                     SVREKESVDLSDLWEIIHRGYMPRLVSSNDDWETFYASYVSTYIERDVNKISHIDDKT
                     DFVRFMASVAARSGELLNYDSISKDIGVSSTTVKRWLQILQTAGIIFLLYPYYSNHLK
                     RMIKTPKVYFMDTGLMAWLTRWVTPDTIKYGAKAGQFFETFVVSEIIKSLLNAGKSTR
                     DLYFYRDADQKEIDLIIQDGRTIYPVEIKMTARPHKKMASAFSVLESVKADDMQIGNG
                     AVINLYYKPLLIDRNVLSIPAGYL"
     gene            2268..3341
                     /locus_tag="PKJ65_03435"
     CDS             2268..3341
                     /locus_tag="PKJ65_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40925.1"
                     /translation="MLDTSVYALNDAQLRRYEELEKEYMQMFTDPKNCIPKFTIPIKT
                     SFNPTWEEILADKDVMLKNHLERNARHVEVGDDSTLNVRVDFGTCVVANAFGCDVFYP
                     TNSLPCAKDHVVKTREQIFSLKPPDISVAPYRKFREWSEYFVEKLPEGYHMEFPDIQG
                     PFNNAHLIRGNDIFFDMYDDIEAFDKLMEVATDATIEYAKATRQWTNAKEGWLYDWSA
                     LWKGNARISNCSLHMIGRELYLNHVIKHDVRFIKELNGARIHYCGSAKNVIEEMAKIP
                     GITGIDYESLLHDIEETMENVPEDLTILQPLGLSTDVAKKILSSKTWPFKKRNIIFRL
                     RGPDKTVEEAKELYRRLRRVAEN"
     gene            3417..>3683
                     /locus_tag="PKJ65_03440"
     CDS             3417..>3683
                     /locus_tag="PKJ65_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ40926.1"
                     /translation="MYIAGIDIGGTKCAAVLGKCVDNNIIRVDIVDKISFKTKSCDSP
                     SAVIDRLILGLESILAKNFLISNDLSCIGISCGGPLDSEKGLILS"
BASE COUNT         1198 a          657 c          773 g         1055 t
ORIGIN      
        1 cacggagtgt tggatatgtt cagaaatccg aaagatgccg cttatgtata cacatcacag
       61 accgataaat atccggtaat gcatgttgca acatccatgt cgaaaggcga ttatcctatg
      121 gcaatgacaa aaagcgtatg ggccttcacc aattgcgatt atataaagat gagtataaac
      181 ggaaagcata taaaagactt ttatcccgac agaaagcgat tcaaacattt attacaccct
      241 cctatagaaa tagatgactt cacgggaaat atgttaattg acgatttgca tttttccgaa
      301 tattcatcaa gggtaattaa aaaacttttg tcacatatat atgtaaacgg accggaaaat
      361 cttccgctcg gatatatgct gatgcttgtg acggtactta taaaagaaaa acttacaata
      421 aacaaactga cggaaataat cactgctcat gtggcgggtt ggggagacaa tgtaaaacag
      481 tatgtgtttg aaggcattaa ggacggaaaa acggtaataa ccgaaattaa agaacctatg
      541 aataagatgc aattagtctt atcggcagat gctcatgaat tgaaagaaga agaaacttac
      601 gataccgcat cggtaaccat aagagcggtg gatcagaata tgaacctttt aaattactgc
      661 aacgagcctt tgcgaattgt taccgaaggt cctataaaac ttataggtcc cgaacttatc
      721 tcgctaaagg gaggttgtgc cggggtatat gtaaaaaccg ataaacaaag cggcaaggcg
      781 gttttaagag taatcggaaa ggatgtaacc gccgaaaagg agtttagcgt gagctgattt
      841 acaaatatcc tgccggtatg gaaagtacat ttctgtcaat caacaggggt ttgtaataca
      901 gattaataac cgctccgttt ccaatctgca tatcatccgc cttcactgat tctaaaacac
      961 tgaaagccga tgccattttt ttatgaggtc tggcagtcat tttaatttca acaggatata
     1021 ttgttctgcc gtcctggata ataagatcaa tctctttttg gtcggcatct ctgtagaaat
     1081 ataaatcacg tgtggatttg cctgcattaa gtaatgactt tataatttcg gaaactacga
     1141 aggtttcaaa gaattggcct gctttagctc cgtattttat ggtatcggga gtaacccatc
     1201 ttgtcagcca agccattaat cccgtatcca taaaatatac tttaggcgtt tttatcatac
     1261 gtttcagatg attactgtaa tacggataaa gcaggaaaat gatgcctgct gtttgcagta
     1321 tttgaagcca tctttttact gttgtggaag aaacaccgat atctttcgat atactgtcat
     1381 agttcaataa ttcgccgctt cttgctgcta ccgatgccat gaagcgaaca aaatccgttt
     1441 tatcatcaat atgtgaaatc ttgttaacat cacgctcaat gtatgttgaa acatagcttg
     1501 cataaaaggt ttcccaatca tcgtttgaag aaaccagtct cggcatatat cctctgtgta
     1561 ttatttccca aaggtctgat aaatctacgc tttctttttc gcggacggaa agatattgct
     1621 cggtaggaag aaaaggaagt gcacaggata tctttttttg ctcacgataa gatatccctt
     1681 gaagaggcaa tattgccaat cttcccgcta aagattcgct gacattctgc attaattcat
     1741 atgcttgcga accggacaac agatataagc attcttgggg tttgttgtta cttaatgcct
     1801 caaatctcat attgtcgatt cttatcttca aagaagaaaa caattccgga acatattgta
     1861 tttcatccag aatcaatttt tgtgaatggc tcataagaaa caactcagga tcgctttttg
     1921 cctgttgcag aataagggcg ttatcaaagg ttatatactc aaaatccttt gcaaccattt
     1981 gtaacaaaga tgactttcct acctgtctgg gacctgttaa taaaacggca gggaaaaaat
     2041 ccattgcatg aagaagatga tttgtaatgg ttctgtcaat ccagttcatg gtaacctcct
     2101 tgtatattta tattccacat atattttaat gcattattgc gtataatgca acatgtcatg
     2161 tcggattata cgcagttttc cggaatatat attccaaacg tcaatgacat aatgtataaa
     2221 acagcaggta atggtataat ttattcagta cgggaaggga gaggcttatg ttggatacat
     2281 cggtttatgc tcttaatgac gcacaattaa gaagatatga agagctggaa aaagaatata
     2341 tgcaaatgtt taccgatcct aaaaattgca tacctaaatt tactattcct ataaaaactt
     2401 cgtttaatcc cacatgggag gaaatacttg ccgataaaga tgtgatgctt aaaaaccatc
     2461 tggagagaaa tgcacgccat gttgaagtcg gggatgattc cactttgaat gtaagagtgg
     2521 atttcggaac atgcgttgtt gcaaacgctt tcggctgtga tgtgttctat ccgactaaca
     2581 gtctgccctg tgcaaaagac catgtggtga aaacgcggga acagattttt tctctgaaac
     2641 ctcccgatat cagtgttgct ccttacagaa agttcaggga atggagcgaa tactttgttg
     2701 agaaactgcc ggaaggatac catatggaat ttcccgatat tcagggaccg ttcaacaatg
     2761 cacacctcat aagaggcaac gacatatttt ttgatatgta tgacgatata gaagcctttg
     2821 ataaacttat ggaggtggct accgatgcca ccatagaata tgcaaaagcc acaagacagt
     2881 ggacaaatgc gaaagaggga tggctgtatg actggtcagc tttatggaaa ggtaatgcca
     2941 gaatatccaa ctgctctttg catatgatag gaagagagct gtatttaaac catgtgatta
     3001 aacatgatgt aaggtttatt aaagaactga acggggccag aatacattat tgcggttcgg
     3061 caaagaatgt aatagaagaa atggcaaaaa taccaggcat taccggtatt gattatgaga
     3121 gcctcttgca tgatattgag gagactatgg aaaatgtgcc ggaagactta accattctgc
     3181 aacctttggg attatccacc gatgtagcaa agaagatact ttcttccaag acatggccgt
     3241 tcaagaaaag aaatatcata ttccggttaa gaggacccga caagaccgtt gaagaggcta
     3301 aagagctgta cagaaggtta agaagagtgg cggaaaacta agctttacag aatttcctaa
     3361 tgtagctgta cagatgatat aattgaagcg aatcatttat aggacggagg tatgtcatgt
     3421 atattgctgg gatagatata ggcggcacaa aatgtgcggc ggtattggga aaatgcgtag
     3481 acaataatat tattcgagtg gacatagtgg acaagatttc attcaagacc aaatcctgtg
     3541 actcaccgtc cgcggtaata gacagactta tcttaggtct ggagagtata cttgcaaaaa
     3601 actttttgat tagtaatgat ttatcctgca taggtataag ttgcggaggt cctttggaca
     3661 gcgaaaaagg ccttatacta tca
//
LOCUS       DAOPWC010000116         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25511_length_3680_cov_3.736552, whole genome shotgun sequence.
ACCESSION   DAOPWC010000116 DAOPWC010000000
VERSION     DAOPWC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25511_length_3680_cov_3.736552"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..517)
                     /locus_tag="PKJ65_03445"
     CDS             complement(254..517)
                     /locus_tag="PKJ65_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNZ40927.1"
                     /translation="MPQATVSFRIDSKLKEEMEQTCKDMGLTMTSAFTMFATKVVRER
                     RIPFEVSADPFYSKENIAELERRVANLNAGISVLKAHELIEVD"
     gene            complement(561..1478)
                     /locus_tag="PKJ65_03450"
     CDS             complement(561..1478)
                     /locus_tag="PKJ65_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNZ40928.1"
                     /translation="MPDILTHILFAKNVKDAISDINVKDAIRNNIQLYNFGAQGPDFL
                     YYHITPLFFDNRVPAAGAMMHKIETAKFFKDFVLWLQNLDGSAYQQGLAYFAGFLTHF
                     YCDKTLHPYVLATAKQGANYFNSSTGKVYLSHYMVEYITDIRLWREKIGIDAYKQDIT
                     ELIGTEPLPYEIVEYITEFINYTQPNAVTKKEVYNSAVKMIRIQKAFYDPKNIKKWWI
                     NLLPLPRKCYVEKAKEDVDVLNVNRRIWSHVMNEDEKSDSSVYDLMEIGCNECVKVLD
                     EISEMLERGIGKDLNKLIPDVGYLTNKPV"
     gene            complement(1471..3105)
                     /locus_tag="PKJ65_03455"
     CDS             complement(1471..3105)
                     /locus_tag="PKJ65_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40929.1"
                     /translation="MKSHYPDIVKGNEAAIENDLKRFNPDERIIYGAYGFPDYANNAV
                     PIETMSSGYYCKDSKFYRDKNLLYIMNCLIDYLYSAQREDYTVDTKISDYKTGPVLLS
                     NFLVRGYRIMKKYAKTEEEIALRDRLKAYLVPVAKGVCNGGTITPNHRWMYSSSALML
                     YNEVKMECLKEISEAYLNEGVDIDEAGEYTERSPMYNRECNNAFIIIAEETKNDYYLE
                     SVRKNLELMLYYIEPDFTMFTQNSNRKDKQEGDVSATFYPDGYYHSYLHMSYLDKNPL
                     FAGMADEIFNSIKKRTGRGPSVLWMYLLNPKLKEYEPKTVTRPASFNIFHKDIVRRKN
                     EDFSVTLLTRSPNFLFVQKGNLRCFVRVCASFFAVAQLIPQKIEKTGDDTYRFTMTAE
                     GKYHGPLPEKPNTVVWKEMDHSKRPVINRVQLTYHVTIALKDTGVCMDIKSEGMDQVP
                     YKVEFCFTAPCTVKNKDKLYDCLPGEHMTFNEGDIDIEKDGNILTITNTHNSHIYHDI
                     MRGSVPKSASSFTIYYTGFTHINEHIEILNQRRENA"
     gene            complement(3102..>3680)
                     /locus_tag="PKJ65_03460"
     CDS             complement(3102..>3680)
                     /locus_tag="PKJ65_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40930.1"
                     /translation="QIGDLRCFVRICASFFAVAQFKPLSIELIGKDTYRMKMKAHGEY
                     YGPLPVKPATTVWKDMDHSLRPVNNKVDLEYIVTVKIKPDRVSLNVKTEGCDNVPCKI
                     EFCITPPVDVQYDSVELKGLPDECLSVNAKEIKIKKENDCLTLTNSFSEHTYHKDMRG
                     SVPPCKGVFTVYYTSFTHIDASVDIIGAKETK"
BASE COUNT          988 a          820 c          651 g         1221 t
ORIGIN      
        1 attccgtaaa caatggatag tataattaaa aaagcaatct gacacgcaac aaacagaata
       61 ataaaattaa tgatagtttt acggtaaagc ttttcactct ctttcatagt tgttttcatg
      121 attacctcac ttcttgccta ttaatgaaga tgcacttact gattaaactt ttcgtaaaaa
      181 ctatttacaa aagtgatcat actccacacc atatactccg ataatcggtc ttatgcttgc
      241 caaattttaa gcatcagtca acctctataa gttcatgtgc tttaagaacg gatataccgg
      301 cattaagatt tgccacacgc ctttccagct ccgctatgtt ttctttgcta taaaacggat
      361 cggcactaac ttcaaaggga attcttctct ctcttaccac ttttgttgcg aacatggtaa
      421 atgcagaggt catcgtaagt cccatatcct tgcaggtttg ttccatttct tctttaagtt
      481 tgctgtctat tctgaaacta actgttgcct gaggcattct ccttctcccg tccgctattt
      541 gtatatacaa tttatatgaa ttatacaggc ttatttgtca gataacctac atcgggtata
      601 agtttattga ggtctttgcc tattcctctt tccagcattt cgcttatttc atccagcacc
      661 ttgacacatt cattacaccc tatttccata agatcgtata cagacgaatc ggacttctca
      721 tcctcattca taacatggct ccaaatgcgt ctgtttacat tcaatacatc cacatcctct
      781 tttgcctttt ccacataaca ttttcttggt aacggtaaaa gattaatcca ccatttcttt
      841 atgttcttgg gatcatagaa tgctttttgt atgcgtatca tttttactgc cgagttgtat
      901 acttcctttt ttgtcaccgc attgggttga gtgtagttta taaactcggt tatatactcc
      961 actatttcat acggcagcgg ctccgtccct ataagttccg ttatgtcctg tttgtatgca
     1021 tcaataccga tcttttctct ccacaacctt atatcggtta tatattccac catgtagtga
     1081 gacagataca ctttaccggt gcttgagtta aaatagtttg ccccttgttt tgctgtcgcc
     1141 aacacataag gatgaagagt cttatcacaa tagaaatggg ttaggaaacc ggcaaaatat
     1201 gccagcccct gttgataagc ggaaccgtcc agattctgca accacaatac aaagtcttta
     1261 aagaatttag ccgtttcaat tttgtgcatc attgctcccg ctgccggcac tctgttatcg
     1321 aaaaaaagcg gagttatgtg ataatagagg aaatccggac cctgtgcgcc gaaattatac
     1381 agctgaatat tgttcctgat ggcatccttg acattaatat cgctgatggc atctttaaca
     1441 ttcttggcaa aaagtatatg tgtcaatatg tcaggcattt tctctccttt gattcaatat
     1501 ctcaatatgt tcatttatat gcgtaaatcc cgtgtaatat attgtaaaag agcttgcact
     1561 cttaggtacc gaacctctca taatgtcatg ataaatatgg ctgttgtgag tatttgttat
     1621 agtcagtatg ttaccgtctt tttcaatgtc aatgtcgcct tcattgaagg tcatatgttc
     1681 tcccggcagg caatcataca gtttgtcttt gttctttacg gtacagggag cggtaaagca
     1741 gaactccacc ttatacggaa cctggtccat gccttccgat tttatatcca tacaaacacc
     1801 tgtatcctta agagcaattg taacatgata agtcaattgt actctgttta taacaggtct
     1861 tttggagtgg tccatttctt tccatacaac tgtgttcggt ttctccggca gaggtccgtg
     1921 atatttgccc tcggcggtca ttgtaaaacg gtaagtgtca tcacccgttt tctctatttt
     1981 ttggggaata agctgtgcca ccgcaaagaa ggatgcacac actcttacaa aacaccgtaa
     2041 attgcccttc tgtacaaaaa ggaaattggg ggatcgggta agtaatgtta cggaaaagtc
     2101 ctcattcttt cttcttacta tatccttatg gaatatgtta aaagaggcgg gacgggtaac
     2161 ggttttcggc tcatactctt taagtttcgg attgagtaaa tacatccaca gaaccgaagg
     2221 tcccctgcct gtcctttttt ttatactgtt aaaaatctca tcggccattc cggcaaataa
     2281 aggattctta tccaaataag acatgtgcag gtatgaatga taatatccgt cgggatagaa
     2341 ggttgcactc acatccccct cctgcttgtc ttttctgttg gagttttgtg taaacatggt
     2401 aaagtccggc tctatgtagt acaacatcag ttccaggttt tttctaacgc tttccagata
     2461 atagtcattt tttgtttctt cggcaatgat aatgaaagcg ttattgcact ctctgttata
     2521 catcggagat ctttcggtat actctcccgc ctcatctata tccacaccct catttaaata
     2581 tgcctccgaa atctctttaa ggcactccat cttaacctcg ttgtacagca taagcgccga
     2641 tgaggaatac atccatctgt ggttgggtgt aatagtacct ccgttacata caccttttgc
     2701 taccggaaca agataagctt tcagtctgtc cctaagagca atttcttctt cggtcttagc
     2761 atattttttc atgattctgt atcctcttac aagaaaattc gataagagta caggtcccgt
     2821 tttgtaatcg cttatcttgg tgtccacagt gtagtcttca cgctgagcgg aatacaggta
     2881 atctatgagg cagttcatta tatacaacaa atttttatca cggtaaaact tactgtcttt
     2941 gcagtaatag ccggaagaca tggtctctat aggtaccgca ttattggcat agtcgggaaa
     3001 accgtaagcg ccgtatatga tacgctcatc gggattaaag cgttttaagt cattctcgat
     3061 agccgcctcg ttgcctttga ctatgtccgg gtagtgcgat ttcattttgt ctccttcgcg
     3121 cctattatgt caacagacgc atctatatgc gtaaaactag tataataaac agtgaatacg
     3181 cccttgcagg gaggtaccga acctctcatg tccttatgat atgtgtgttc ggaaaaactg
     3241 tttgtaagcg taagacaatc attctctttt ttaatcttaa tctcttttgc atttaccgat
     3301 aaacactcat cgggcagccc tttaagttcc acagaatcat attgtacatc caccggagga
     3361 gttatgcaga actctatctt gcacggaaca ttatcgcatc cttccgtttt tacattcaga
     3421 ctcactctgt cgggtttaat ctttaccgta actatatatt ccaaatccac cttgttatta
     3481 accggtctta aggaatgatc catgtccttc catacggtag ttgccggttt taccggcaga
     3541 ggcccgtaat actcgccgtg cgctttcatc ttcatccgat atgtatcctt accgataagc
     3601 tctattgaca aaggcttgaa ttgagccacg gcaaagaaag aagcacaaat ccttacaaaa
     3661 catctcaaat cacctatctg
//
LOCUS       DAOPWC010000117         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25591_length_3671_cov_2.810288, whole genome shotgun sequence.
ACCESSION   DAOPWC010000117 DAOPWC010000000
VERSION     DAOPWC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25591_length_3671_cov_2.810288"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKJ65_03465"
     CDS             complement(<1..1277)
                     /locus_tag="PKJ65_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40931.1"
                     /translation="MPKDNIQSFVDIYNKNFVNGQKLKMILGVTLKVLMDDNTAIFNP
                     DKTEISRNLCAGYLKEDNFFVHEISDNSVKRSEVLKKNDYIGIRNFCENKAIYIFSEA
                     DDDYVSSMLAKNGITVINVKKLFEILENKTYTVSTLLLRLNASSDNIASAAYAVAVIL
                     KKSFDTYEDICIKIADNNVKNDNLNVYPVSVSVMNREGLRNLYRILTKRNCMWSIVPK
                     SYLEAHRYGLLLGSSDTEGELYSMLINDAATETVYAKTIFYDYVNLMTDRHAELLKEK
                     NTIKTINEFRNFNKFLYKCAVECNVIAIATCFSKDISKSEFMTTNEMLSEFSYLDKDN
                     ANKVVIENTAYLSGQAKFVSPVTEADYKDDIISIAYLTNLNDKSEAYEKEFKLKTKEK
                     TAEIIILIGKMIEKLKDNDILFRPESVPTPYIL"
     gene            complement(1314..2243)
                     /locus_tag="PKJ65_03470"
     CDS             complement(1314..2243)
                     /locus_tag="PKJ65_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40932.1"
                     /translation="MLNNDINIIKFRYEKENETLFVELSAKRYIFFREESELIKEIRS
                     IYNLGKEFAKIVFDFIKVSFDKEKTDEYIKDIYENLFNEFNYFILYKSTMNYETKNDT
                     VYIFLPKEAGMIADISKINAYLSGCSKRLGNAVRFIISETVNEDATKIRDASVAGIQN
                     EIPDVVVLPPKEEEIEQPSGRKITRICELDSNSYKVVIEGTITYVKIHKNQGYQLFEF
                     GVNDLSGSIVCKKFFSREKDKDTIDKYENIIVENKYVNVSGRHQIEKNSPRYFINADK
                     VTEVRLGVRTDKARVKRMEMNIDTDHVNADLLL"
     gene            complement(2237..>3169)
                     /gene="ispG"
                     /locus_tag="PKJ65_03475"
                     /gene_synonym="gcpE"
     CDS             complement(2237..>3169)
                     /gene="ispG"
                     /locus_tag="PKJ65_03475"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ40933.1"
                     /translation="EQITRLKDAGCDIVRISVFDNDCVSNIPLIKKAVKIPLVADIHY
                     NHMLAIDSIKAGIDKIRINPGNITDKNRIKAIIDTAKEYKVPIRIGVNTGSLPEHLYE
                     MYDGPAAQAVIHAVRDQVDMFLNYDFDNIVIAAKSSDAMQTVEIYRMLNQAFDFPLHV
                     GVTEAGSYIKGTIKSTVALSILLEEGISDTIRISLTDDPLKEVWVANDILNFMGIKKY
                     GVNVISCPTCGRTKIDIIRIASVIEERTKHIRKNIDVAVMGCAVNGINEAKYADIGIA
                     GGEKKAVLFKKGKILRTVPEDRIIEELLIEIEKC"
     assembly_gap    3170..3179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3180..3307)
                     /locus_tag="PKJ65_03480"
     CDS             complement(<3180..3307)
                     /locus_tag="PKJ65_03480"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ40934.1"
                     /translation="MNFNEKRKLKKEIKIADITIGGKNDIAIQSMTNTDTRDINATV"
     gene            complement(3307..>3671)
                     /locus_tag="PKJ65_03485"
     CDS             complement(3307..>3671)
                     /locus_tag="PKJ65_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ40935.1"
                     /translation="IVDIIFSIVWLITGIVRMSEMMGPIGIISTMGEQIKQPTFVLFI
                     LNLVNMMAFISINLGIMNLIPFPALDGSKIVIHSVEWIMGKPFNQDKMKYVTLAGFIL
                     LILLLVFVTFNDIVRLVK"
BASE COUNT         1046 a          734 c          534 g         1347 t
ORIGIN      
        1 cttaatatat acggagtcgg aaccgattcc gggcggaaga gtatatcatt atctttaagt
       61 ttttctatca ttttcccaat cagtattatg atttcggcgg ttttctcctt ggttttcaac
      121 ttaaattctt tctcatatgc ctccgactta tcatttaagt tggtcagata tgcgatactg
      181 ataatatcat ccttataatc cgcttcggta acgggagaaa caaattttgc ctgcccactg
      241 agatatgccg tattttcaat aacgaccttg ttcgcgttat ctttatccag ataggaaaat
      301 tccgacaaca tttcatttgt agtcataaat tccgacttgg aaatatcttt tgaaaaacat
      361 gtagcgatgg caataacatt acactcgaca gcacatttgt ataaaaactt attaaaattt
      421 ctgaactcat ttatagtttt tatagtattc ttctctttta acaactcggc atgtctgtct
      481 gtcatgagat ttacataatc atagaaaatc gttttggcat aaacggtttc ggtggcggca
      541 tcgttgataa gcatcgaata caattcgcct tccgtatcgg aagaacctaa taataaaccg
      601 tatctgtgtg cttcaagata gctcttcggt actatgctcc acatacaatt tcttttggtc
      661 agaattctat ataaatttct taaaccttct ctattcataa ctgaaacgga aaccggatat
      721 acgttcagat tgtcgttttt tacattatta tctgcaattt ttatacatat atcttcataa
      781 gtatcaaagg atttttttaa tattacggcc acagcatatg cagcacttgc tatattatcc
      841 gatgatgcgt ttaatctgag taaaagagta cttaccgtat atgttttatt ctccaatatt
      901 tcaaataatt tctttacatt tataacggtt attccgttct ttgccaacat tgaagaaaca
      961 taatcgtcat cggcttccga gaaaatataa atagccttat tttcacagaa atttcttata
     1021 ccgatataat cgttcttttt taaaacctca gatcttttta ccgagttgtc tgatatctca
     1081 tgaacaaaaa aattatcttc ttttaaataa ccggcacata aatttcggga aatctctgtc
     1141 ttatcgggat tgaatatcgc ggtattatca tccatcaata ctttgagcgt aacacctaaa
     1201 atcattttca gcttttgtcc gttaacaaag tttttattat atatatccac aaatgattga
     1261 atattatctt taggcattat accgaaagcc ttatgtttcc atctgtttaa ttgttataac
     1321 agcaagtcgg cattaacatg gtcggtatct atattcatct ccattcgctt tactctggct
     1381 ttatcggttc tgacacccaa tctgacttcg gttactttgt ctgcatttat aaagtatctg
     1441 ggtgaattct tttcaatctg atgccttccc gaaacattga catatttgtt ttccacaata
     1501 atattttcgt atttatctat tgtatctttg tctttttctc tgctgaaaaa ctttttacat
     1561 actatggaac cgcttaaatc atttacaccg aactcaaaca actgatatcc ttgattctta
     1621 tgtattttca cataagtgat tgtaccttct attactactt tgtaggagtt actgtccagt
     1681 tcgcagatac gtgtaatttt ccttcccgac ggctgctcaa tctcctcttc tttaggcgga
     1741 agcactacta catcaggtat ttcattctgt ataccggcaa ccgatgcatc ccttatttta
     1801 gtcgcatcct cattgactgt ctcagaaata atgaatctta cggcatttcc cagacgtttt
     1861 gaacaaccgg aaagataagc atttatcttg gatatatcgg caatcatacc tgcttctttc
     1921 ggcaagaaaa tgtatacagt atcgtttttt gtttcgtagt tcattgtaga tttatagaga
     1981 atgaaataat tgaattcgtt aaacagattt tcatatatat cttttatgta ttcatctgtt
     2041 ttttctttgt caaacgatac ttttataaaa tcaaaaacaa tttttgcaaa ttcttttcct
     2101 aaattgtata tcgaccttat ttcttttatt aactcacttt cctcacggaa aaatatatat
     2161 ctctttgcgg acaactcaac gaaaagggtt tcgttttcct tttcatatct gaattttatt
     2221 atatttatgt cattattcaa catttttcaa tctcaataag cagttcttct atgattctgt
     2281 cttccggtac ggttctgagt attttgcctt ttttaaagag caccgctttt ttttcaccgc
     2341 ctgcaatacc tatatcggca tatttcgctt cgtttatacc gttaaccgca catcccatta
     2401 cggcaacatc aatatttttt ctgatatgct ttgttctttc ctcaatgact gaagctattc
     2461 tgattatatc aattttagtt cttccgcatg taggacagct tattacgttg accccgtatt
     2521 tctttatacc cataaaattc agtatgtcat tagcaaccca aacctccttt aacggatcat
     2581 cggttaatga gattcttatt gtatcggaaa tgccttcctc aagcagtatg gataatgcaa
     2641 cggtagattt tattgtccct tttatataac ttcccgcctc ggttacaccg acatgaagcg
     2701 gaaaatcaaa tgcttgattg agcattctgt atatttctac agtctgcatt gcatcggagg
     2761 attttgcagc aatgacaata ttatcaaaat catagttcaa gaacatatcc acctggtctc
     2821 ttaccgcatg tattaccgct tgggctgcag ggccgtcata catttcatac agatgctccg
     2881 gtaaagaacc tgtatttaca cctattctta taggaacttt atactccttt gcagtatcaa
     2941 taatagcttt tatgcggttc ttgtcggtta tattaccggg atttatcctt attttatcaa
     3001 taccggcttt aatactgtca attgccagca tgtggttata gtgtatatcg gcaacaagag
     3061 gtattttaac ggctttcttt atcaacggaa tattacttac acaatcatta tcaaaaaccg
     3121 atattcttac aatgtcacaa cccgcatcct ttaaccgggt aatctgctcn nnnnnnnnna
     3181 cggttgcatt tatatctctt gtatcggtat tggtcatgga ctgaatggca atatcgtttt
     3241 ttccgcctat ggtgatatcc gctatcttta tctctttttt taattttctc ttttcgttaa
     3301 aattcatcat ttaaccaacc tgacaatatc attaaatgtt acaaatacaa gcaacagtat
     3361 aagcagtata aatcccgcca gtgtaacata cttcattttg tcctgattaa aaggttttcc
     3421 cattatccat tctacggaat gtataacaat tttggagccg tcaagtgcgg gaaacggtat
     3481 caggttcata attcccagat ttatggatat aaatgccatc atatttacaa gatttaaaat
     3541 gaataacaca aatgtaggct gtttaatctg ttctcccatt gtagatatta taccgatagg
     3601 ccccatcatc tccgacatcc tgacaattcc ggtaataagc cagactattg aaaatataat
     3661 atctacaatg g
//
LOCUS       DAOPWC010000118         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25631_length_3666_cov_3.964553, whole genome shotgun sequence.
ACCESSION   DAOPWC010000118 DAOPWC010000000
VERSION     DAOPWC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25631_length_3666_cov_3.964553"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="PKJ65_03490"
     CDS             complement(<1..1774)
                     /locus_tag="PKJ65_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40936.1"
                     /translation="MNAKIKCYNNRPTIFIDGKPVPPIIYALTDWIGGRLSYDEITGF
                     SIKKFAAAGIRLYQLDISFQDMWFEDGTFDISIALKQIKGIIDVRPDAAVFFRLHINP
                     PRWWYKGKESEWVRYANTEVYPEPDIELARTYILHDLKPVPRASFASEKWMNDMTIMV
                     RRFLEELYKSPLSEHLAGIQVANGVYGEQHYWAFMRNDPDLSEPMLKRYRKFKNDPNA
                     QIPGMEERYNPKDGIFFDPEKDSNLIDYLTCQHEAVAESIIHFCKLVKDTWKREIITG
                     VFYGYYVSMFGRAGLGGHLAEEMILQCPYVDFLSAPQAYNSNLRHIGGAGVARGLLES
                     ARLNNKLFLDEMDHPTELGVLHGGMKVYPPYESLQILRRNTLVSFLSGMGFWYYDFGP
                     FNTSGWWYEPYYLDEIKSLQKIYNKYYEKEYIKRADVLLVFDTKVQKYTALTENADPI
                     TDKACINVAYPMALRSGASMDTIYLSDLEKTNLDKYKCIIFMNTFALTDTQKQYITKK
                     VYKKGRSIVWFFAPGYTDKKRNDIAFCKQISGFDLYVIAPAPQITVQCKGFSYSVDNS
                     DKESKLNKLFVPKDGNILGYIGKTP"
     gene            1915..2001
                     /locus_tag="PKJ65_03495"
     tRNA            1915..2001
                     /locus_tag="PKJ65_03495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1949..1951,aa:Leu,seq:cag)
     gene            2185..3525
                     /locus_tag="PKJ65_03500"
     CDS             2185..3525
                     /locus_tag="PKJ65_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020760061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40937.1"
                     /translation="MMKRKIYNDLLLWKDKSKGKTALLIDGARRVGKSYIAELFAKQE
                     YKSHIIIDFGNAPQSILDLFINESSDLDLFFAKLAAFYSTVLHERESLIVFDEVQQFP
                     RARQLIKYLVADGRYDYIETGSLIRLKKNTENIIIPSEEEHIEMFPMDFEEFLWAMGD
                     EATVPLIRECFDKRKPLGQALHRKIMNDFRQYMLVGGMPQSILAYRNGKNFEAADIAK
                     RRILQLYRDDVSKFAAGYEEKVYAVFDGIPGQLSKKEKKYTLSAIDKNARFRTYEDTF
                     VWLSEAMIVNNCYNTTDPNVGLALSADYSTQKCYIADTGLLVTHTFMDRSFTENELYK
                     NILFDNLYINEGMLMENIVAQMLRRNGHKLFFYSRSDSRNRENHMEIDFLIREKNKIS
                     AVEVKSANYRSHSSLDKFKRKFAGKIGSSYILYPKDVMEKNDIIHLPLYMAMFL"
BASE COUNT         1090 a          708 c          735 g         1133 t
ORIGIN      
        1 ccggagtctt gcctatatat cccaatatat ttccgtcttt tggtacaaac agcttattca
       61 atttgctctc tttatcactg ttatctacgg aataggaaaa gcctttacac tgtacggtta
      121 tctgcggcgc aggtgcaata acatacagat caaatcccga tatctgctta cagaaagcta
      181 tgtcatttct ctttttatct gtatatccgg gagcaaagaa ccatacaatt gaacggcctt
      241 tcttataaac tttcttggtt atatactgct tctgtgtatc ggtaagtgcg aatgtgttca
      301 tgaatattat gcatttatac ttatccaaat ttgttttttc caaatcgctt aagtatatgg
      361 tgtccatgga agctcccgat ctcaatgcca tcggataggc tacgtttata caggctttat
      421 cggtaatggg gtcggcattt tccgtaagag cggtatattt ctggactttg gtatcaaaca
      481 ccaaaagcac atccgccctt tttatatact ccttctcata gtatttgttg tatatcttct
      541 gtaacgactt tatctcatcc agataatacg gttcatacca ccagcccgat gtgttaaacg
      601 gtccgaaatc atagtaccaa aagcccattc ccgaaaggaa ggaaactaaa gtatttcttc
      661 ttagtatctg caatgattca taaggaggat atactttcat tcctccgtga agaaccccta
      721 attcggtagg gtggtccatc tcgtcaagga acagtttgtt gttgagtctt gccgattcca
      781 gcaagcctct ggctacacct gctccgccta tatgccttaa attgctgttg tatgcctgag
      841 gtgccgataa aaagtccaca tagggacatt gcagtatcat ctcttccgca agatgtccgc
      901 ccaatcccgc tcttccgaac atggaaacat aataaccgta gaaaacgccg gttattatct
      961 ctcttttcca tgtgtcctta acaagcttac agaagtgaat gatggattcg gcaacggcct
     1021 catgctgaca ggtaagataa tcaatcagat tggagtcttt ctccgggtca aagaatatgc
     1081 cgtccttcgg attgtatcgc tcttccattc cgggaatttg tgcgttcgga tcatttttaa
     1141 actttctgta tctttttaac ataggttcag aaaggtcggg atcatttctc ataaaagccc
     1201 agtaatgttg ctctccgtac actccgtttg ctacctgtat tcctgcaaga tgctcggata
     1261 aaggtgattt atagagttcc tctaagaatc ttcttaccat gattgtcatg tcgttcatcc
     1321 atttttccga agcgaagctg gctctgggta cgggtttcag atcatgaaga atataggttc
     1381 tggccaactc tatatccggc tcgggataaa cttcggtatt tgcatatctg acccattcgc
     1441 tttctttgcc tttataccac catctcggag gattgatgtg cagtcggaag aataccgccg
     1501 catcaggtcg gacatctatt attcctttaa tctgctttaa agctatggag atatcaaaag
     1561 taccgtcttc aaaccacata tcctgaaagg agatatccag ctgatacagc cttatacctg
     1621 cggctgcaaa tttctttatg gaaaacccgg ttatctcatc gtaacttaat cttcccccta
     1681 tccagtcggt aagagcataa atgataggcg gaaccggctt gccatcaata aatattgtcg
     1741 gtctgttgtt gtaacattta atcttggcat tcatgacgga aactccttaa ttgatatatg
     1801 taaacaataa cataatgtac aaagtaatca agcacaaaac tgcatatttg agaacatagt
     1861 caacacaata aatcattgac tatacggtga ttatgctcta aaatggtaaa gcaagcggat
     1921 gtggcggaat tggcagacgc gctagattca ggttctagtg attgcgagat tgtgcaggtt
     1981 caagtcctgt catccgcacc aaaagacggt gattgaaata tttcagttgc cgtttattta
     2041 ttcactaata ttgttctttt taaaaagaaa attgaacgag gttgcttatt cgtgcaaatt
     2101 tgccgttaat gaggttgctt tttcgtgatt tgcatgatac tatactttcg tacaacactg
     2161 taactattgg tggagattat ggaaatgatg aaacgaaaaa tatataacga cctattgttg
     2221 tggaaagaca aaagcaaggg gaaaaccgca ttacttattg atggagcacg ccgtgtaggg
     2281 aagagttaca tagctgaact ttttgcaaag caggagtata aaagtcatat tattatagac
     2341 ttcggtaatg ctccgcagag tatactcgat ctttttatca atgagagttc cgatttggat
     2401 ttattttttg ctaagctggc agcattctat tcaacagttc ttcacgagag agaatctttg
     2461 atagtctttg atgaagtgca acaatttccg agagcacgac agcttattaa atacttggtt
     2521 gctgacggtc gctatgatta tattgagaca ggctcactta taaggctgaa aaagaatacg
     2581 gagaacataa tcattccttc cgaagaagaa catattgaaa tgtttccaat ggattttgaa
     2641 gaattcttat gggctatggg agacgaggcg acagttcctc tgattcggga atgcttcgac
     2701 aaaaggaaac cgttaggtca ggcattacac cgtaaaatta tgaatgattt cagacaatat
     2761 atgctggtag gaggtatgcc gcagtccata cttgcttata ggaatgggaa aaactttgag
     2821 gctgcggata ttgcaaaacg caggattttg cagctatatc gtgatgatgt atcaaagttt
     2881 gctgcgggct acgaagaaaa agtatatgct gttttcgacg gtattccggg tcagctttca
     2941 aagaaggaga agaagtatac actttcagct attgataaaa atgcacgctt tcgcacctat
     3001 gaagatacat tcgtctggtt gagcgaagcc atgatagtta acaattgtta caatacaacc
     3061 gatcctaatg taggtctcgc actgagtgca gattacagca cacaaaaatg ctatatagcc
     3121 gatacaggtt tacttgtcac gcatactttt atggatagat cctttacgga aaatgaactt
     3181 tataaaaaca ttctatttga caatctctac atcaacgaag gcatgttaat ggaaaacata
     3241 gttgcacaga tgcttagaag aaatggacat aagctctttt tctattcccg cagcgatagc
     3301 agaaatcgcg aaaatcatat ggaaattgat tttttaataa gagagaaaaa taaaatttca
     3361 gcagttgaag taaagtccgc taattatcgt tcccattctt cactggataa atttaagagg
     3421 aaatttgccg gtaaaatagg cagctcctat attctttatc caaaggatgt catggagaaa
     3481 aatgatatca ttcatctacc gttgtatatg gctatgttcc tataggtagt agatttgcaa
     3541 atatctggaa aactgattga tagagcaact ccttatagac tatttatctt tttctgttac
     3601 aattttcata gaggtgaaca tgtcgctgaa gaaggtaaca ttgaggttga atataaagta
     3661 cggaat
//
LOCUS       DAOPWC010000119         3664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_25661_length_3664_cov_3.330285, whole genome shotgun sequence.
ACCESSION   DAOPWC010000119 DAOPWC010000000
VERSION     DAOPWC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25661_length_3664_cov_3.330285"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PKJ65_03505"
     CDS             <1..1749
                     /locus_tag="PKJ65_03505"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ40938.1"
                     /translation="GWNQTGYQDCDWITPFISAIPYPMSYTLVPQITPTVSFSISYPV
                     EIIKKKKGNYLLDFGQEICGNTVITAKGKKGHVITVWHAEELTEDGKSARYNMRCNCD
                     YEETITLSGKEKDKAEFFDFKGFRYIEVFNYPGKLTKKSIYVHHRHYPIPKNHSKTQS
                     DNTLFQRIWEMCEKGIIEGTQESYLDCPTREKGAFLGDGLVTGLAHLAYTNDSRIMKK
                     FLYDCMHSARLCPGLFCTTVTYDASQIIDYSLVFPVMLNKYFEYTEDISVLKDSLNVL
                     EGIIAYYAQFYRPEGILGHIKHVHREQYTVLVDWPAVYRDGYSDEITKGPNCFGNLAY
                     IGILKESAKVYSYTGNELRAKELSKKAKQLEKAVINAFYDNETGLFNITKTSDIHNFH
                     DAVFALYFDLELPKGKKPLLDYIKRRGIVCGVYFTYYYFSVLYNHKEYDKIFDLIVSD
                     EKNSWKTMLNTGATTCIEAWHPDDKKNCSFCHPWSSTPMIFNDIFFGISSYGTGYKVL
                     QFKPRIPKQFNKGTYTKPTPLSDITVSFERKDKKIIYNVTLKKACRMYVCLSDKEEGY
                     FADASVGEHKFIQITD"
     gene            complement(1746..3452)
                     /gene="argS"
                     /locus_tag="PKJ65_03510"
     CDS             complement(1746..3452)
                     /gene="argS"
                     /locus_tag="PKJ65_03510"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNZ40939.1"
                     /translation="MKDIKTKIAKKLAGSIEQLSWTEIMDNLEYPPEPHMGDLALPCF
                     KLSRVMRKSPMAIADSLVDSLDIDVVERCESKSGYLNIFIKKEYLVNNVLKTIVEKKE
                     ETGKSNEYAGKTVCLDYSAPNIAKPFHIGHLRSTVIGHAIRRLHEFRGYRCVGINHLG
                     DWGTQFGKLIVAYKKWSSKKAVEEKGTEELVRIYVKFHEEAEKDPSLNDLARQWFARL
                     ENKDEDAMELWKWFKKISLIDIEKTYKVLDITFDSYAGESFYYDKTQPIVERLKERNL
                     LIESDGAMIVNLEEYNMPPCLIVKSDGASLYASRDIAAAIYRKETYDFDKCIYVTATA
                     QDLHFRQWFKVVELMGYEWAKNLIHVGFGMVSMGGAKLATREGNIVYLEDLFSEASKR
                     VLDIINEKNPDMEDKEKKAQDIGVGAVVFSDLSNNRIKDIDFSWEEALNFDGSTGPYV
                     QYTYARACSVLRKSELSEKDINMKDIKPEHMTNDDEFTLLKTLSLFNEKIDQALRDLE
                     PSCISRYLIDVCMDFNKFYNAHSILGAEDNKKFRILLTAAVKYVLANGLFIIGLKRTE
                     RV"
BASE COUNT         1125 a          781 c          652 g         1106 t
ORIGIN      
        1 ggatggaacc aaacggggta tcaagattgc gattggatta ctcctttcat atctgcgata
       61 ccttacccca tgtcctacac ccttgtaccg cagataaccc ctactgtttc ctttagtatt
      121 tcttatcccg tggagattat aaaaaagaaa aagggcaatt accttctgga tttcggtcag
      181 gagatatgcg ggaataccgt aataacggct aaaggtaaaa aagggcatgt cattaccgta
      241 tggcatgcgg aagaattaac tgaagacggt aaatccgcaa gatacaatat gcgatgcaac
      301 tgcgactacg aagaaacaat taccttaagc ggaaaggaaa aagacaaagc cgagttcttt
      361 gactttaagg gatttcgata tattgaagta ttcaattatc cgggtaaact tactaaaaaa
      421 agcatatatg tgcatcacag acattatccc ataccgaaga atcattccaa gacgcaatcg
      481 gataacactt tattccaaag gatatgggag atgtgcgaaa aaggtattat tgaaggtacg
      541 caggaatcat atttagactg ccccacaaga gaaaaaggag cctttctggg agacgggctg
      601 gttaccggtc ttgctcatct tgcctataca aacgattcga gaataatgaa aaaatttctt
      661 tatgactgta tgcactccgc aaggttatgc cccgggctgt tctgcacaac ggttacttac
      721 gatgcatcgc agataataga ttattctctt gtttttccgg tcatgctgaa taagtatttt
      781 gaatacaccg aagatatatc cgtactaaaa gattctctta atgtattgga aggcataata
      841 gcatattatg ctcagtttta ccgacctgaa ggaatattgg gacatataaa gcatgtacac
      901 agagaacaat atacagtgct ggtagactgg ccggctgtat acagagacgg atatagcgac
      961 gagataacaa aaggccccaa ctgtttcggc aaccttgcat atatcggaat actcaaagaa
     1021 tcggcaaaag tatattccta tacgggcaat gagctaagag caaaggaact gtcgaaaaaa
     1081 gcaaagcaac ttgaaaaagc tgtgattaac gctttttatg ataatgaaac aggcttattc
     1141 aatattacaa aaacctccga tattcacaac ttccacgatg cggtattcgc cttgtatttt
     1201 gacttggaac ttcctaaagg caagaagcct ttattggatt atataaagag aagagggata
     1261 gtatgcggag tatatttcac atattactat ttctccgttt tatacaacca taaagagtat
     1321 gacaaaattt tcgaccttat agtttccgat gagaaaaact cctggaagac catgctcaat
     1381 accggtgcta caacctgtat agaagcatgg cacccggacg acaagaaaaa ttgcagtttt
     1441 tgccatccgt ggtccagtac acctatgatt tttaatgata tattcttcgg tatatcgtca
     1501 tacggtacgg gctataaggt attgcagttc aaaccgagaa ttcccaaaca gtttaacaag
     1561 ggaacatata caaagcctac tcccctttcg gatataacgg taagttttga aagaaaagat
     1621 aaaaagataa tatacaatgt tactttaaag aaagcgtgca gaatgtatgt ctgcctgtcg
     1681 gacaaagaag aaggatactt tgcagatgca tctgtaggag agcacaagtt tattcagata
     1741 accgactaaa ctctttccgt ccttttcaga cctatgataa acagtccgtt tgccaaaaca
     1801 tatttaaccg ccgccgtaag cagtattctg aactttttgt tatcttcggc acccagtatg
     1861 gagtgtgcat tgtagaactt attgaaatcc atacagacat caataagata gcgggatatg
     1921 caggaaggtt ccagatctct caatgcctga tctattttct cattgaataa agacaatgtc
     1981 tttaagagtg taaactcgtc atcattagtc atatgttccg gtttaatatc tttcatattg
     2041 atatcttttt ctgacagctc ggattttctc aataccgagc atgctcttgc atatgtgtac
     2101 tgcacataag gaccggtgga accgtcaaag ttcagcgctt cttcccagga aaaatctata
     2161 tcttttattc tgttattgga taaatcggaa aacactacgg cacctacgcc gatatcctgc
     2221 gctttcttct ctttatcttc catatcggga ttcttctcgt tgattatatc caacaccctt
     2281 ttagatgctt cactgaaaag atcctcaaga tatacaatat ttccctctcg cgttgccagt
     2341 tttgcaccgc ccatggaaac cattccgaat cccacatgaa taagattctt tgcccattcg
     2401 taacccataa gctccacaac cttgaaccat tgcctgaaat gaaggtcctg tgcggttgcg
     2461 gtaacatata tgcatttatc gaaatcatat gtttcttttc tgtatatagc ggcggcaata
     2521 tctctggatg cgtacaagga agctccgtcg gattttacta taagacaggg aggcatatta
     2581 tactcttcca aatttacaat catggcgccg tcgctttcga ttaaaagatt tctctctttc
     2641 aatctttcca ctatcggctg cgttttatca tagtagaaag attcacccgc atagctgtca
     2701 aaggtaatat ccaacacttt atatgtcttc tcaatatcta taagactgat ttttttgaac
     2761 catttccaaa gctccatagc atcttcatct ttattctcca gccttgcaaa ccattgtctt
     2821 gccaggtcat ttaatgaagg gtctttttcc gcttcctcat ggaattttac atatatcctt
     2881 accagttctt ccgttccctt ctcttcaaca gcctttttgg aactccactt tttatacgca
     2941 acgatgagtt tgccgaactg agtaccccag tcacctaaat gatttatacc tacacatcta
     3001 tatcctctaa actcatgaag ccttcttatg gcatgtccta ttacagtgga acgcagatgt
     3061 cctatatgga aaggttttgc aatattggga gcggaataat ccagacaaac agtctttccc
     3121 gcatactcat tgctctttcc tgtttcttct tttttctcca ctatggtttt taaaacatta
     3181 ttaaccaaat attctttttt aatgaatata ttaagatagc cgctcttact ctcacatctt
     3241 tccacaacat caatatccaa agaatccaca agcgaatcgg caatggccat cggagatttt
     3301 cgcatcactc tgcttaactt aaagcaaggt aatgcaagat cacccatatg cggttcagga
     3361 ggatactcca ggttatccat aatctccgtc caactcaact gttcaatgct gcctgcaagt
     3421 ttcttcgcta tcttggtctt tatatctttc ataaccgtct tctttcatta ataacttaat
     3481 attataaaac cataccctat attaacataa cgcaacaagg ttttccaata aatatgtgtt
     3541 tgggttaaag ttaagacttt aaggtgttat tagtttcatc agtcaaaact gaagtgaatg
     3601 tttgtaagct gcactttacc gtcaaccacc gttatatccg cattaaaaga tcctgcatac
     3661 caag
//
LOCUS       DAOPWC010000120         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26093_length_3620_cov_3.130154, whole genome shotgun sequence.
ACCESSION   DAOPWC010000120 DAOPWC010000000
VERSION     DAOPWC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26093_length_3620_cov_3.130154"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PKJ65_03515"
     CDS             complement(<1..1395)
                     /locus_tag="PKJ65_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ40940.1"
                     /translation="MLEKYLQRTEFVSYEDFKQNYKVIVPDNFNFGFDIVDAWAEQDK
                     DMPALVWCDDFQNEKYFTFTDIKKLSNQAANYFISLGIKKGSKVLVILRRRWEYWIIA
                     VALHKIGAILIPASLQLTKKDIAYRANAADVQAIICVNDDFVISQTESAFPDCPKVMN
                     LILVNRDNSTDRNNGSTISEIWKDFSKEAFRCSDVFKRPAGKEATKTKDTMLLYFTSG
                     TTGMPKMVHHDYSYPLGHIVTAKYWQCVEKGKLHMSVSDSGWAKFGWGKIYGQWICGA
                     VIFAYDMDRFIPANLLKMMEKYKLTTFCAPPTMFRFMLQEDVKQYDLSSIKRFCIAGE
                     PLNPEIFRKWKEITGKPLTEGFGQSESSVMIANFKWIEPKPGSMGKPAPLYDIQLINA
                     DGIACEDGEEGEITVRNMESEHPVGLFLGYYKDTELTKQHIQNGVYNLKDVAWRDNQG
                     YYWFVGRHDDVIKCS"
     gene            complement(1404..1952)
                     /locus_tag="PKJ65_03520"
     CDS             complement(1404..1952)
                     /locus_tag="PKJ65_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ40941.1"
                     /translation="MEETMEIARRIAGLRDACGYSQEDMAKELNIDIETYRDYERNGQ
                     NIPISVIFRIANNFKVDLSEILTGTSAKLDTYHMVKAGHGKEVDRYPGYNYTDLAFRY
                     SKKIMQPLLVTLDPSDEPAALVSHTGQEFNYVISGKMAFSFDDQQIILDEGDCIYFNA
                     THPHGQSCASDVPVVFLTVIAE"
     gene            complement(1952..2485)
                     /locus_tag="PKJ65_03525"
     CDS             complement(1952..2485)
                     /locus_tag="PKJ65_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ40942.1"
                     /translation="MSTLNILLAGFGGQGILFLGKMAAYSGLMDNKQITWLPSYGPEM
                     RGGTANCSVCISDDLIGSPLVINPDILVAMNLPSLEKFIDKVVPGGTVLVDCSIIDRK
                     VHREDINVFYVPATRLATDNGLPGLANMIMLGKMFKETGFCTECALEEALSACVPPRK
                     AEMMELNRKALKIGSEL"
     gene            complement(2482..3231)
                     /locus_tag="PKJ65_03530"
     CDS             complement(2482..3231)
                     /locus_tag="PKJ65_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ40943.1"
                     /translation="MAVVFQKPQALLDVPFTYCPGCTHGIIHRLVAETIDELGIEGHT
                     VGVAPVGCSVMNYNFFACDMVQAPHGRAPAVATGLKRAIPENIVFTYQGDGDLAAIGT
                     AETVHAAVRGENITVIFVNNAIYGMTGGQMAPTSLPHQVTQTTPYGRDTSYAGHPIRV
                     CEMLATLEGTALAQRVSVDNVKNVQNAKKAIKKAFTYQIEKRGFSIVEVLSTCPTNWG
                     LDAQESLQWLRDNMIPYYPLGLYKDVGGTTK"
     gene            complement(3231..>3620)
                     /locus_tag="PKJ65_03535"
     CDS             complement(3231..>3620)
                     /locus_tag="PKJ65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ40944.1"
                     /translation="RYEKYEVIKKEEQMADEYLTDDADIVLVAYGASSRICRTAVNTA
                     RKEGIKAGLIRPITLWPYPAKYIEKHIAHAKSFLCVEMSMGQMVDDVRLCVNGRKPVA
                     FYGRTGGIIPAPDEVLNEIRKLTGGTK"
BASE COUNT          870 a          884 c          721 g         1145 t
ORIGIN      
        1 cgaacatttt atgacatcat catgtcttcc tacaaaccag taatatccct ggttgtcgcg
       61 ccatgccacg tctttaaggt tatacactcc gttctgaatg tgctgctttg taagctccgt
      121 atctttataa taccctaaga ataaccctac cgggtgctcg ctttccatat tgcgtacggt
      181 tatttctcct tcttcaccgt cttcgcaggc aataccgtcc gcatttataa gctgtatgtc
      241 atagagagga gcgggttttc ccatggagcc gggcttgggt tcaatccact taaaattggc
      301 tatcataacg gaagattccg actgaccgaa gccttcggta agcggttttc cggtaatctc
      361 tttccacttt ctgaatattt ccggattgag cggttccccc gcgatacaga aacgctttat
      421 ggaagaaaga tcatattgct ttacatcttc ctgaagcata aacctgaaca tggtaggggg
      481 agcacagaag gtggtcagtt tgtatttttc catcatttta agaagattgg cgggtatgaa
      541 cctgtccatg tcatatgcga atattacggc accgcatatc cattgcccgt aaatcttgcc
      601 ccaaccgaac tttgcccaac cggaatcgga tacgctcata tgcagttttc ctttttccac
      661 gcattgccag tactttgcgg taactatatg tcctaaaggg taggagtaat cgtgatgtac
      721 cattttgggc attcccgttg tgccggaagt aaagtacaac agcatggtgt ctttagtttt
      781 tgtggcttct tttcctgcag gtcttttgaa tacatcggag catctgaaag cctctttaga
      841 aaaatccttc catatctcac ttatagtact tccattgttt ctatcagtgc tgttgtccct
      901 gtttacaagt ataaggttca taactttcgg acagtcggga aaagcgctct cggtctgtga
      961 tattacgaaa tcatcgttta cgcaaatgat tgcctgcaca tctgcggcat ttgccctgta
     1021 agctatatct tttttggtaa gctgcaacga agcgggtatg agtatggcac ctatcttgtg
     1081 taatgccacc gctattatcc agtattccca tcttcttctt aagattacaa gtaccttgga
     1141 gcctttttta atgcctaaac ttatgaagta attggccgct tgattggaca gttttttaat
     1201 gtcggtaaaa gtaaagtact tctcgttttg gaagtcgtca caccagacca aagccggcat
     1261 gtctttatct tgctctgccc atgcatccac aatatcaaat ccgaaattga aattatccgg
     1321 aacaattacc ttgtagtttt gtttgaagtc ttcataggag acaaattccg tcctttggag
     1381 gtatttttct aacatattta ctcctattcc gctataaccg taagaaacac tacgggcaca
     1441 tccgatgcac aactctgacc gtgaggatgg gttgcattga aataaatgca gtctccttca
     1501 tccagaatta tctgttgatc atcgaaggaa aatgccatct ttccgcttat aacatagttg
     1561 aactcctgac cggtatggct taccagagcg gccggctcat ccgacggatc cagtgttacc
     1621 aataacggct gcattatctt tttggaatag cgaaaagcca gatcggtata gttgtatccg
     1681 ggatatctgt ccacttcctt gccgtgtccg gcttttacca tatggtatgt gtccagttta
     1741 gccgatgttc cggtaagtat ctcggaaaga tccactttaa agttatttgc tatacgaaat
     1801 atgacggata taggaatatt ttgtccgttt ctctcataat cgcgatatgt ttcaatatcg
     1861 atatttagct cttttgccat atcttcctgt gaatatccgc aggcatctct caatcccgca
     1921 attcttcttg caatctccat tgtctcttcc attacagttc actcccgatc ttaagcgctt
     1981 ttctgttcag ttccatcatt tccgcttttc ttggcggaac acatgccgat aaagcttctt
     2041 ccaaagcaca ctctgtgcaa aagcctgtct ctttaaacat ctttcccagc attatcatat
     2101 tggccagtcc cggcaatccg ttgtcggttg ccaggcgtgt tgcaggtaca tagaaaacat
     2161 ttatatcttc tctgtgcact ttcctgtcta ttattgagca atccactaaa acggtgcctc
     2221 cgggtactac cttatcaata aatttctcca aagagggaag attcatggcc accaatatgt
     2281 cgggatttat aaccagcgga gagcctatca ggtcgtcgga tatgcagacc gaacagtttg
     2341 cagttccgcc tctcatttcc ggaccgtagg agggaagcca ggttatctgt ttgttatcca
     2401 taagtcccga atatgcggcc atcttaccta agaacaatat gccctgacct ccgaaacctg
     2461 ccagaagaat attcagtgta ctcattttgt tgtacctccg acatccttgt atagtcccaa
     2521 cggatagtag ggaatcatgt tatctctcag ccattgcagt gattcttgtg catccaagcc
     2581 ccagttggtg gggcaggtgg acagcacttc cactatggaa aagcctcttt tttctatctg
     2641 gtaggtaaaa gcctttttta ttgccttttt agcattttga acattcttta cgttatctac
     2701 cgatactctt tgtgccagtg ccgtcccttc cagtgttgcc agcatttcac atactcttat
     2761 gggatgaccg gcataagaag tatctcttcc gtacggagtg gtctgtgtta cctgatgagg
     2821 taaactggtg ggagccatct gacctcccgt catgccgtat atggcattgt ttacgaatat
     2881 aacggtaatg ttctcgcctc ttaccgctgc atgaacggtt tccgccgttc ctattgccgc
     2941 cagatccccg tcaccctgat atgtgaacac tatattttcc ggtatggctc tttttaaacc
     3001 ggttgcaacc gcaggagctc tgccgtgcgg agcctgcacc atatcgcagg caaagaaatt
     3061 atagttcata accgaacagc ctacgggagc cacccccacg gtgtggcctt ctattcccag
     3121 ttcgtctatc gtttccgcta ccagtctgtg tattattccg tgagtacatc ccggacaata
     3181 tgtaaagggt acatctaaaa gtgcttgggg tttttgaaat actacagcca ttattttgta
     3241 cctcccgtta attttcttat ctcgttcaat acctcgtcgg gtgcaggtat tatgccgccc
     3301 gtccgcccgt aaaaggcaac cggttttctt ccgttgacac aaagccggac atcatccacc
     3361 atctgtccca tgctcatttc cacgcataag aaagatttag catgagcgat atgtttttct
     3421 atatacttag cgggataagg ccataatgtt atcggtcgga taagtcccgc ctttattcct
     3481 tcttttcttg ccgtattaac cgcggttctg catattctgg acgaagcccc gtatgcaacc
     3541 agtactatat cggcatcatc ggtcaggtat tcatccgcca tctgttcttc ttttttaata
     3601 acttcatatt tctcatatct
//
LOCUS       DAOPWC010000121         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26373_length_3593_cov_2.811193, whole genome shotgun sequence.
ACCESSION   DAOPWC010000121 DAOPWC010000000
VERSION     DAOPWC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26373_length_3593_cov_2.811193"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..829)
                     /locus_tag="PKJ65_03540"
     CDS             complement(149..829)
                     /locus_tag="PKJ65_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ40945.1"
                     /translation="MNISLNYAEKGEGDILILLHGNSESNKIFKHQIDYFSSKYRVIA
                     IDTRGHGNSPRGNAPFTLGQFAEDLNNFFTEHDIKKANILGFSDGGNIAMIFTIKYPE
                     KVSKLIINGANLNPDGVKGYFNIFLRFMYKIFKGLSKISKNSNKYLEMMSLMVNEPDI
                     DVNALKKISVPTLVIAGTNDLIKEEHTRLIASAIPDSELIFIKGDHFIVSKKPKEFND
                     VVNSFLNN"
     gene            971..2929
                     /locus_tag="PKJ65_03545"
     CDS             971..2929
                     /locus_tag="PKJ65_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40946.1"
                     /translation="MKKKTQKNSAKKSKTKTKPKKAISKFSYSMGKIILVILFVAVVA
                     LALYFILDGSLKLINKRHIYNEKSVGALVMAVEKNDYDKAVEIYEQLSEEDKISLHNN
                     QSFLTAIDNKFSEVLSYEYDSDKNDYKEENEYKFVQYFCRFLHPATVRENVYYFFNTF
                     KLYNSPPYENFKNFIGILNELLKDGNFEEQINLYNNEVEAIRESREQYFKGKVYEEQQ
                     DYNNAYLCYANVVIEDTYAYELAKEDIARIKGLMAKEILDQARTFESEGDLENAYYTI
                     KSAPDLLSDVPEIVEYREYITNLYQRANYVPYTGVVYNIFFHSLALYPDIAFSSSRGR
                     ELFDIMTTRYEFRKTLEKLYEHGFMLINVTDIYEIYIKDGKEHLKIKEQLLLPEGKKP
                     LIISFDNMACTHASVGFCKMLTLDDSNNLASIVTIEGVDTLTYDGEHVLILYDFVKKH
                     PDFSYNNAMAVIGMSGYESMFGYATADLNSPSRKSELDKAKKVADKLKEMGYVFANHS
                     YYHYTKSSDIPESYTDLQWNTYDTNLWKEHIEPILGKTNIYITPGGKNYSVAKYIDGD
                     RTDSCYNYLVDAGYQIILSVGRGQFYANNIIGVANPRFFFGTSLFMDRYNIDGKSFYK
                     TDVVLEQVFGFPYSDIIDPIREKYKQSY"
     gene            complement(2993..>3593)
                     /locus_tag="PKJ65_03550"
     CDS             complement(2993..>3593)
                     /locus_tag="PKJ65_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ40947.1"
                     /translation="QLTEKVRRNPYSLLLFDEIEKAHPDVFNILLQILEDGRLTDSKG
                     RVVKFENTIIIMTSNAGTSYKNTGFGYIQDKTDTSDRVREALKEIFRPEFLNRVDDVI
                     VFTSLSEQEIFEIGRLMAEEYAEDLKEKNILLKVSDDALKHIIGENYDDKYGARPLRR
                     SMQTNIEDVLSEMYIKNELKDGMMVMISVEDGTLKYECI"
BASE COUNT         1155 a          638 c          587 g         1213 t
ORIGIN      
        1 ctgtacaatt catatttaat ccctaaccag taatggatat aatttgttcg cttacgtata
       61 ttatatcgtt ttattctata ttgcaaatgt taattgtcat tatatttaaa aaagtatact
      121 ttgacagaca tattacagga aaatcttttt aattgtttag gaatgaatta actacatcat
      181 taaattcttt cggctttttg gatacaataa agtggtcacc ttttatgaat atcaattctg
      241 aatcgggtat tgcggatgct ataagccttg tatgttcttc cttaataaga tcatttgtcc
      301 ccgctattac caatgtcggt acagatattt tcttcagagc attcacatcg atatccggct
      361 catttaccat cagactcatc atttccaaat acttattcga attcttactg atttttgaca
      421 aacccttaaa aattttatac atgaatctta agaatatatt gaaatatcct tttactccgt
      481 caggattcag atttgctccg ttaataataa gtttcgaaac tttttcgggg tattttatag
      541 taaatatcat tgcaatatta ccgccatcgg aaaatcccag aatatttgct ttctttatat
      601 catgttccgt aaagaagtta ttcaagtctt cagcgaattg accaagagta aacggcgcat
      661 ttcctcttgg cgaattgccg tgtcctcttg tatctatcgc aataacacga tattttgaac
      721 tgaaataatc tatctgatgt ttaaaaatct tattactttc actgttaccg tgaagaagta
      781 ttaaaatatc cccttctccc ttttctgcat agtttaatga tatattcatc tttttgtccc
      841 ccggatgatt atatcataat ttaatcatct caatattgtt cagatttttt accgaatcat
      901 tgtgtgcata tttatttttt tccgttatgg tggtaaaata ttatcaaatt gatatacaaa
      961 ggaaaaagac atgaaaaaga aaacacaaaa gaatagtgca aagaaaagca aaacaaaaac
     1021 caaaccgaaa aaggcaatat ccaagttcag ctatagcatg ggcaaaatta ttcttgtaat
     1081 tctttttgtt gcagttgtgg ctttagccct ttatttcata cttgacggat cgctgaaatt
     1141 gataaacaaa agacatatat acaatgaaaa atccgtcggt gcacttgtta tggccgtaga
     1201 aaagaatgat tatgacaagg ctgtggaaat ttatgagcaa ctgtcggagg aggacaaaat
     1261 ttctcttcat aacaatcagt cttttttaac cgctattgac aataaatttt ccgaagtact
     1321 ttcctatgaa tatgacagtg ataagaatga ttacaaagaa gaaaatgaat ataaattcgt
     1381 acagtatttc tgcaggtttt tacatcctgc taccgtaagg gaaaatgttt actatttctt
     1441 taacacattc aaattgtaca attcccctcc gtatgagaat tttaagaact tcatcggtat
     1501 attgaacgaa ttactgaaag acggcaattt tgaggagcag atcaatcttt ataacaatga
     1561 agttgaagcc ataagagaat ccagagaaca atattttaaa ggcaaagtat atgaagaaca
     1621 acaggactac aacaatgcat atctgtgtta cgcaaatgtt gttattgaag atacttatgc
     1681 ttatgaactt gctaaggaag atatagccag aataaaaggt ttgatggcaa aagaaatact
     1741 tgatcaggcc agaacatttg aaagcgaagg cgatttagaa aatgcatact atacaataaa
     1801 atccgctccc gatttacttt cggatgttcc ggaaattgtt gagtatagag aatatattac
     1861 caatctctat cagagagcca actatgttcc atatacagga gtggtctata atattttttt
     1921 tcattctctc gccctttatc ccgatatagc cttctcctct tccagaggac gggaattgtt
     1981 tgacataatg acaacaagat atgaatttcg gaaaacactg gaaaaactgt atgaacacgg
     2041 attcatgctc ataaatgtta cggatatata tgagatttac ataaaggacg gaaaagagca
     2101 tttaaagatt aaagaacaac tgctgctgcc cgaaggtaaa aaacccctta taatctcctt
     2161 tgataatatg gcctgtacac atgcaagcgt aggtttctgc aaaatgctta cactggatga
     2221 tagtaacaac ttagcttcaa tcgttacgat agaaggagta gacactctta cctatgacgg
     2281 tgagcatgta ctgatacttt atgattttgt taaaaaacac ccggattttt cctataacaa
     2341 tgctatggct gttataggta tgtccggtta tgaaagtatg ttcggatatg ccacggcaga
     2401 tttgaattca ccatcccgta agtcggagct ggataaagca aagaaagttg ccgacaagtt
     2461 aaaagagatg ggttatgttt ttgccaatca ctcttattat cattacacta aaagttccga
     2521 tattccggaa tcttatacgg atttacaatg gaatacttac gatacaaatt tgtggaagga
     2581 acatatagaa ccgattctcg gaaagacaaa tatatatata acgcccggcg ggaaaaatta
     2641 ttccgtggca aaatatattg acggtgacag aaccgactcc tgttataact atcttgtgga
     2701 tgctggttat cagattattt taagtgtggg aagaggtcag ttttatgcaa ataatatcat
     2761 aggtgtcgct aatcccagat tcttcttcgg aacttccctt tttatggaca gatataacat
     2821 tgacggtaaa agtttttata aaaccgatgt ggtacttgaa caggtattcg gattccccta
     2881 ttccgatata attgacccta taagagaaaa gtataagcag tcctactgat tatactttct
     2941 taactatttt tacatttgat tttatatagt attaaaatct ataagtgatt atttaaatac
     3001 actcgtattt taatgttccg tcttcaaccg atatcataac catcattccg tctttcagtt
     3061 cattctttat atacatctcc gaaagaacat cttcaatgtt ggtctgcatg cttcttctta
     3121 acggtcttgc accgtattta tcatcataat tctcaccgat tatatgtttt aacgcatcat
     3181 cgctgacttt cagaagaata ttcttttctt ttaaatcttc tgcatattct tccgccataa
     3241 gtctgcctat ctcgaatatt tcctgttcgc ttaaacttgt aaatacaatt acatcatcca
     3301 ctctgttaag aaactccgga cggaatattt cctttaatgc ttctcttact ctgtccgatg
     3361 tatcggtctt gtcctgtata tatccgaatc cggtgttttt atatgatgtt ccggcattgg
     3421 atgtcattat gattatggtg ttttcaaact taacaactct tcccttgctg tcggtcagtc
     3481 ttccgtcttc taaaatctgt aacagtatat tgaatacatc gggatgagct ttttctatct
     3541 catcgaataa aagcaaggaa taaggatttc tccgtacttt ttccgttaac tga
//
LOCUS       DAOPWC010000122         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26564_length_3573_cov_2.709210, whole genome shotgun sequence.
ACCESSION   DAOPWC010000122 DAOPWC010000000
VERSION     DAOPWC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26564_length_3573_cov_2.709210"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKJ65_03555"
     CDS             <1..773
                     /locus_tag="PKJ65_03555"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNZ40948.1"
                     /translation="LCEEYAKKGGYRIYVAGGDGSVNQAANGVAGYDTPIGILPTGSG
                     NDFIKNLTDYAKLKDLVKRTVEGDVIKVDAIKLYDEELFGYCINILSIGVDAEAAYNS
                     TQITRKYGITGMLAYLLGFFRVLRQKKTKFDVKITIDGQVINDGYVMLAACTNGKYYG
                     GGFIPVPHTSFDDGIIDMCIAEARGLDFVFSVLPKYMKGKHAGTVGIIFPKGKSISLE
                     SKDMLKVNIEGEIIERRSIRMEIMPRLISFIKPKDLQE"
     gene            complement(778..1113)
                     /locus_tag="PKJ65_03560"
     CDS             complement(778..1113)
                     /locus_tag="PKJ65_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40949.1"
                     /translation="MEKNEDLGTRLENAGKRFFRKTKEFADATKLSIKLKEYELDLDN
                     LYQIAGKAAYYGEDTKEIFTQITNKLEQIEEVKVDIADAKGLKICGNCKAEMPKEAVA
                     CMKCGEKFE"
     gene            complement(1140..1586)
                     /gene="mscL"
                     /locus_tag="PKJ65_03565"
     CDS             complement(1140..1586)
                     /gene="mscL"
                     /locus_tag="PKJ65_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNZ40950.1"
                     /translation="MAKKNKTNLLSEFKAFITKGNVIDMAVGVIIGAAFGKIVSSFVN
                     DILMPLIGLVMGKVNFHDLKWVLATDAEGNITSSVNYGTFLQGILDFLIIAACVFLLV
                     RLVALVRSKAEEAKQKQKEEVKATPPAPSKEQVLLTEIRDLLKEKK"
     gene            1971..2528
                     /locus_tag="PKJ65_03570"
     CDS             1971..2528
                     /locus_tag="PKJ65_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ40951.1"
                     /translation="MDYYDINIAGVQRKLPLCKVTDNLYIAAFVVFGDIELTIACAKR
                     LLELAPEYDYTITAESKGIPIVHEMARQSGVNKYFLARKAPKLYMTGVFEVDVKSITT
                     SGLQKLYLDTADAEFMKGKRILIVDDVISTGGSLRAVEELVLQAGGIVAGKMAVLAEG
                     DAIYRNDITYIAPLPLFNPDGTIKR"
     gene            2525..>3573
                     /locus_tag="PKJ65_03575"
     CDS             2525..>3573
                     /locus_tag="PKJ65_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNZ40952.1"
                     /translation="MIRKNKDTITTVDAKKRAVIIALSLLLLLSGCVKESSLYITEKN
                     GMYSTSFMAMDTVMTVSVISKNAEKELHEAYKHILAYDVAFSAHSQNSEVSKVNNSSG
                     KDIVVSDELKEQIRTALKVSKMTDGAFDITVFPLMELWGFYDKNHTVPDEASIKDILK
                     LVGYDKIGLEGNMLNLQSGTKIDLGAVTKGFVGQKTAAMLIDSGVKSGMLSLGGNIQL
                     FGLNPDKSVWNIAVQDPKDEHSYIGIIHDTNCSIITSGCYQRYFEKDNKIYHHILNPH
                     TGYPADSGILSVTVVANDGTYADALSTALFVTGLDKAVEIWREYGDFEAIFVTEDKHV
                     YITEGLKDKFTPYSC"
BASE COUNT         1105 a          695 c          705 g         1068 t
ORIGIN      
        1 agttgtgtga agagtatgca aaaaagggcg gatacaggat atatgttgca ggcggtgacg
       61 gatcggttaa ccaggccgca aacggagtgg caggttatga taccccgata ggcatacttc
      121 ctacgggaag cggaaacgac ttcattaaga atcttactga ttacgcaaaa cttaaagacc
      181 tcgtaaaaag gacagttgaa ggcgatgtta ttaaagttga cgctataaaa ctgtatgatg
      241 aagaattatt cggttattgc ataaatatat tatctatagg tgtagatgcc gaggcagctt
      301 acaactccac acaaattaca agaaaatacg gaataaccgg aatgcttgca tatcttctgg
      361 gtttcttcag agttttacga cagaagaaaa ccaaattcga tgtcaagata accatagacg
      421 gacaggttat taatgacggt tatgtaatgc tggcagcctg tacaaacgga aagtattacg
      481 gcggaggatt catacctgtg ccgcacacat catttgatga cggtataatc gatatgtgca
      541 ttgccgaagc cagaggtctg gattttgttt tttccgtgtt gccgaaatac atgaaaggta
      601 agcatgcagg tactgtggga ataatctttc ccaaaggaaa aagcatatcc ttagaaagta
      661 aggatatgct caaagttaac attgaaggcg agataataga aagaaggagc atccgaatgg
      721 aaatcatgcc ccgtcttata agctttatta aacctaagga tttacaagaa taatatctta
      781 ttcaaacttt tcgccgcact tcatacaagc tacggcttct ttcggcattt cagctttgca
      841 gttgccgcat atcttcaagc ctttagcatc ggcaatatcc acctttacct cttcaatctg
      901 ttccagttta ttggttatct gggtaaagat ttcttttgta tcttcgccgt aatatgctgc
      961 tttgcctgct atctgataaa ggttatcaag atccaattca tactctttca attttataga
     1021 aagtttcgta gcatcggcaa attcctttgt tttcctgaag aatcgcttac ctgcattttc
     1081 cagtcttgta cccaaatctt cattcttttc catgtttcac ctcctgttat aattatactt
     1141 tacttctttt cttttaacaa atctctgatt tccgttaaaa gcacttgttc cttggaggga
     1201 gcgggcggtg tcgcctttac ttcctctttt tgtttctgct ttgcttcttc agctttactt
     1261 cttaccaatg ccacaagtct taccaacagg aatacgcatg ccgcaatgat aagaaaatcc
     1321 agtattcctt gaaggaatgt tccgtaattc accgatgatg ttatattacc ctcagcatcg
     1381 gtagccaaaa cccatttcag gtcatggaaa ttcactttac ccattaccag ccctattaac
     1441 ggcatcaata tatcatttac aaatgaagat actatttttc cgaatgcggc tccgattatt
     1501 acacctaccg ccatatctat tacattacct tttgttataa atgctttgaa ttcgcttaac
     1561 agattagttt tgttcttctt agccatacac ttatctcctt tatatatttc tctacaactt
     1621 aattttacac tgttgaaaaa caaatgcaac ggttattgat atttatcccg tataagaaga
     1681 aagaccgtca atcgtgcaga acgccgattt cggatctttc aaagactcag ccggcttaga
     1741 aaaaggttat tgcctttacc ggctctatag cgtttttatt atatcattaa tattttcctt
     1801 ttcaagatat tgtttcgaaa gttcacaatt gttcataatc cgatgcacat aaaatttctt
     1861 gtatatgcct tgtataagat tggcttaaat tactgttttc acattctata tatcaccgtt
     1921 atgatgtata atatatatgt tgaaaaagca ttgaaaggat gcgttggttt atggattact
     1981 atgatatcaa tatagccgga gtgcaaagaa aattaccgtt atgtaaagtt accgacaacc
     2041 tatatatagc cgcttttgtg gttttcggag atatagagct gacaatagcg tgtgcaaaga
     2101 gacttttaga acttgcaccg gaatatgatt acaccattac cgccgaatcc aaaggaattc
     2161 ccatcgtcca tgaaatggca agacagagcg gagtcaataa atattttctt gcaagaaaag
     2221 ctcccaaact gtacatgacg ggagttttcg aggtggatgt aaaatccata accacatccg
     2281 gtcttcaaaa actttatctg gacaccgccg atgcggaatt tatgaaggga aaacgtatcc
     2341 tcatagtcga cgatgtgata tctaccggcg gctcattacg tgcagttgag gaattggtac
     2401 tgcaagccgg cggaatagtt gcgggaaaaa tggcggtgct cgcagaaggg gatgccattt
     2461 atcggaatga tataacatac attgctccgc tccccttgtt caatccggac ggaacaataa
     2521 aaagatgata agaaaaaaca aagacacaat tacaactgtt gatgctaaaa aaagagctgt
     2581 cataattgct ttgtctttat tgttgctgtt aagcggatgt gtgaaagaat cttcactgta
     2641 tataacggaa aaaaacggta tgtattcaac ttcttttatg gcaatggata ccgtgatgac
     2701 ggtctcggtt atatcaaaaa atgccgaaaa agaattgcat gaagcatata agcatattct
     2761 ggcatatgat gtcgcctttt ccgctcactc ccaaaacagc gaagtatcaa aagtaaacaa
     2821 ttcatcaggt aaggatatag ttgtatccga tgaacttaaa gaacagataa gaacggcatt
     2881 gaaagtatca aaaatgactg acggagcttt tgacataacg gtgttcccgc tgatggaact
     2941 gtggggattc tatgataaaa accataccgt tccggatgag gcaagtatta aagatatcct
     3001 gaaacttgtc ggatatgaca agattggtct ggaaggtaat atgcttaatc tgcaatcggg
     3061 tacaaagata gatttgggag ccgttacaaa aggttttgta ggacaaaaaa cagctgcaat
     3121 gcttattgat tccggtgtta aatcaggcat gctttctttg gggggcaata ttcaactgtt
     3181 cggacttaac cccgataaat cggtgtggaa tatagccgtt caggatccga aagacgaaca
     3241 ctcctatatc ggaataatcc atgatacaaa ttgctctatc ataacttccg gttgctatca
     3301 gagatacttt gaaaaagaca ataaaatata tcatcatata ctcaatccgc acaccggcta
     3361 tcccgccgac agcggtatcc tttcggtcac cgtggttgca aatgacggca catatgccga
     3421 tgcattatct acagcccttt ttgtaacagg cttagataaa gctgttgaaa tatggagaga
     3481 atacggggat tttgaagcga tcttcgtaac cgaagataag catgtatata tcaccgaagg
     3541 gctgaaggat aaatttactc cgtattcctg tca
//
LOCUS       DAOPWC010000123         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26619_length_3568_cov_3.410760, whole genome shotgun sequence.
ACCESSION   DAOPWC010000123 DAOPWC010000000
VERSION     DAOPWC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26619_length_3568_cov_3.410760"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1879
                     /locus_tag="PKJ65_03580"
     CDS             140..1879
                     /locus_tag="PKJ65_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNZ40953.1"
                     /translation="MLEIYFNSRDTTYKSLFGAVQSGSTVQFRLDVRCDAPVEAYIVI
                     DKSRHRMHLERLIKDLSVFKINIQVSSKPGLMFYHFEISTPYHTVYYGNRSDTCQGQG
                     VKYDKNPLDYQITVYDNYNTPDWIKGRIMYQIFPDRFYKEKIHKSPYWDRIFHSDWYE
                     EPYIEEDFPGGKMECSDFFGGNLQGIIKKLDYIKSLGVGVIYLNPIYLAYSNHRYDAA
                     DYMSVDDTLGTKSDLKKLCEEAKKKDIFIILDMVFSHTGSDSVYFDKHKRFNKDGAFN
                     NPDSPYRSWYTFKQDGTYESWWGIETLPNTVETDPGFMSHVFDSIRQWLSLGVKGFRL
                     DVADELPDEFIRELRKVIKSCDKDAILIGEVWEDASNKVSYGNRREYLYGYELDSVMN
                     YPYKNAIIDYTLNKINAESFVRRIEIIRENYPPQSYECLMNMLGTHDVSRILSIMGGK
                     GNLEKLPRKQQKGVFLSKEEYNIAFKRLSFCLQLLMCLPGVPSVYYADEAGSQGLKDP
                     FNRKTYPWGREDKKILDLYKRIMNNRCNDDKLKYGSFAIREDHGLVIITRKYQNESCE
                     YTFNNRHLSCSHS"
     gene            1987..3315
                     /locus_tag="PKJ65_03585"
     CDS             1987..3315
                     /locus_tag="PKJ65_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNZ40954.1"
                     /translation="MLKITFMGAGSSVFVKNVIGDCMRTEALRDSEFALYDIDGQRLN
                     DSKMLIDSLNKNINDNRAKVTTYLGVKNRKDALRGADFVINAIQVGLYEPCTVIDFEV
                     PKKYGIRQTIADTLGIGGIMRALRTIPVMQDFADDMTAVCPDALFLNYTNPMSMLAGY
                     MQRYTDVRLVGLCHSVQVCSGGLLDKLGMQDKIEGRVDLIAGINHMAWLLEIKDKFGN
                     DLYPEIREKAAIKNATEKHNDMVRFEYIKHLGYYCTESSEHNAEYNPWFIKPAYPELI
                     ERFNIPLDEYPRRCIKQINKWAVQREELLHNTKTTHTRSNEYASYIMDAVVTNKPFKI
                     GGNVLNKGLITNLPEDACVEVPCMVDGNGINPCHVGKLPVQLAAMNMTNINVHLMTIE
                     AAVTRKREDIYRAAMLEPRCAAILSIDDIIKMVDELIETHQAAGFMKEYK"
     gene            3330..3479
                     /locus_tag="PKJ65_03590"
     CDS             3330..3479
                     /locus_tag="PKJ65_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HNZ40955.1"
                     /translation="MSEALLQTITGFSIVVLTLLLLTGIIAIFSKVMAKANAKNNQGV
                     EDENS"
BASE COUNT         1170 a          605 c          806 g          987 t
ORIGIN      
        1 cacatgataa aatagttaga gattatgccg acagacgcaa atggaacaag atggcggtac
       61 tcaatgtggc aaacagcgga cgtttttcct cggataacag tatagccaac tataataaac
      121 tgatttggaa gcttaaataa tgctggaaat atattttaac agcagagata ccacatataa
      181 atcactgttc ggagccgtgc aaagcggttc aaccgtacaa tttcgtctgg atgtcagatg
      241 cgatgctccc gttgaagcat acattgtcat tgacaagtct aggcatcgga tgcatctgga
      301 aaggcttatc aaggatttat ctgtatttaa aatcaatatc caagtttctt caaagccggg
      361 attaatgttc tatcattttg aaatatccac cccttatcat accgtatact acggcaacag
      421 gtccgatacc tgtcagggcc agggtgttaa gtacgataag aatcctttgg attatcagat
      481 aacggtatat gataactaca atacgcccga ttggataaaa ggacgtatca tgtaccagat
      541 atttcccgac aggttttaca aagaaaaaat ccataaaagt ccttactggg acagaatatt
      601 tcattccgac tggtatgaag agccttacat cgaagaagat ttccccggcg gtaaaatgga
      661 gtgttcggac tttttcggag gaaatctgca ggggataata aagaagttgg actatataaa
      721 gtcattagga gtgggtgtca tttaccttaa ccccatatat ttagcttatt ccaaccacag
      781 atatgacgct gcagactata tgagtgttga tgatacctta ggtactaaaa gtgacttaaa
      841 aaaactgtgc gaagaagcca aaaaaaagga tatattcatt attctggata tggtattttc
      901 ccataccgga agtgacagcg tatactttga caaacacaaa agattcaaca aagacggagc
      961 atttaacaat cccgattcac cttacagaag ctggtacact ttcaaacaag acggcacata
     1021 cgaatcgtgg tggggtattg agacattgcc caatacggtg gaaaccgatc cgggattcat
     1081 gagtcatgtg tttgactcaa taagacaatg gttatcttta ggtgtcaaag gtttcaggtt
     1141 ggatgtggcg gacgaattgc ccgatgaatt catcagggaa ttaagaaaag tgattaagag
     1201 ttgtgataaa gatgcaatct taataggtga agtgtgggaa gatgcaagta ataaagtcag
     1261 ttacggcaac cgtcgggaat atctttacgg atatgaactg gacagcgtaa tgaattatcc
     1321 gtataaaaac gcaattatcg attacactct taataaaata aatgcggaaa gctttgtcag
     1381 aagaattgag ataataaggg agaattatcc tcctcagtca tatgaatgtt taatgaatat
     1441 gctgggaact catgatgttt ccagaatact gtcgataatg ggcggaaaag gcaatttgga
     1501 aaaacttccc agaaaacaac agaaaggcgt atttctaagc aaagaagaat ataatatagc
     1561 ttttaaaaga ttgtctttct gtctgcaatt actgatgtgt ctgcccggtg tgccttccgt
     1621 atactatgcg gatgaagcgg gaagtcaggg actgaaagac ccgtttaacc gaaaaactta
     1681 tccctgggga agagaagata aaaaaatact tgacctttac aaaagaataa tgaacaacag
     1741 atgtaatgat gacaaactga aatacggttc ttttgccatc agagaggacc acggtcttgt
     1801 cataattacc cgcaaatatc aaaatgaaag ttgtgaatat acatttaaca atcgtcattt
     1861 gtcctgttcg cactcgtgaa gtgcactttt ttccggcttt ttcagttact ttttttaccc
     1921 tatcaatgtt gattgagttg tgtttgtatg ataacattaa ctttagtgga ttttcgggag
     1981 gttcatatgt tgaaaataac ttttatgggt gcgggaagct cggtattcgt taagaatgta
     2041 ataggcgatt gtatgcgtac cgaagcgctc agagattcgg aatttgcatt gtatgatatt
     2101 gacggtcaga gactgaacga ttcaaagatg ctgattgatt cgttaaataa aaacattaat
     2161 gataacagag caaaggttac aacatattta ggtgtaaaga acagaaaaga tgctctgcgg
     2221 ggtgcggatt ttgtgataaa tgccattcag gtaggcttat atgaaccctg tacggtaata
     2281 gattttgaag ttcctaaaaa atacggcata aggcagacta ttgccgacac attgggaata
     2341 ggcggaataa tgagagcctt acgtaccatt ccggttatgc aggattttgc cgatgatatg
     2401 acggctgtct gtcccgatgc cctgtttctt aactatacaa accccatgtc catgcttgcc
     2461 ggctatatgc aaagatatac ggatgtacgt ctggtaggcc tttgtcattc ggtacaggtt
     2521 tgttcgggcg gattactcga caaattgggt atgcaggaca agattgaagg aagggtggat
     2581 ttaattgccg gaataaacca tatggcatgg ttgctggaga ttaaagataa attcggtaac
     2641 gatttgtatc cggagatacg tgagaaagcg gcaattaaga atgctacgga aaagcataac
     2701 gatatggtca gatttgaata tattaagcat ttgggatact actgtacgga atccagcgaa
     2761 cataatgcgg aatataaccc ctggttcatt aaacccgctt atccggaact tatagagaga
     2821 ttcaatatac ctttggatga gtatcccaga agatgcataa aacagattaa taaatgggcg
     2881 gttcagagag aggaacttct gcataatact aaaacaacac acacaagaag caatgaatat
     2941 gcttcctaca taatggatgc cgttgttacc aataagccgt tcaaaatagg cggtaatgtg
     3001 ttgaataaag ggctgattac caacctgccc gaagatgcat gtgtggaagt tccgtgtatg
     3061 gtggacggta acggtataaa tccttgtcat gtcggaaaac ttcccgtaca gcttgcggca
     3121 atgaatatga ccaatatcaa tgtacacctt atgaccattg aagccgccgt tacaagaaag
     3181 cgggaagaca tatacagagc agccatgctg gaaccgagat gtgcagctat cctgtctata
     3241 gatgatatta taaagatggt ggatgagctt atagagacac atcaggcggc aggttttatg
     3301 aaagagtaca aataaacgga ggaagaattg tgtcggaggc attattacag acgataacgg
     3361 gttttagtat agtagtctta acattactgt tactgaccgg aataatagca attttttcca
     3421 aggttatggc taaagctaat gctaagaata atcaaggtgt tgaggatgag aattcttgac
     3481 taaaaccttg atttattata tatttcacga gaatggcgtt ttggaggtta ggctatgtcg
     3541 gattcattgt tgcagacatt aataggct
//
LOCUS       DAOPWC010000124         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26695_length_3561_cov_2.992584, whole genome shotgun sequence.
ACCESSION   DAOPWC010000124 DAOPWC010000000
VERSION     DAOPWC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26695_length_3561_cov_2.992584"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ65_03595"
     CDS             complement(<1..204)
                     /locus_tag="PKJ65_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40956.1"
                     /translation="MKRYIKAVMFVLVLVLVAAFSVSCADTKENLFSINIINGKEIHI
                     TKYHGKSNRISVPAEIKGLPVTGV"
     gene            411..1832
                     /locus_tag="PKJ65_03600"
     CDS             411..1832
                     /locus_tag="PKJ65_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNZ40957.1"
                     /translation="MKTAILIKDCTLKNCETDKALNISFKEKLEIARQLENINTDIIE
                     TGFADTSQGAFIAVKTIASNLKKSILCCPAQAFKASIDKTYEAIKGAVRPRIHISLPV
                     SSVNMEYQLHMKKNQVVETVKQTISYAKTLFSDVEFTALDATRSDMDFLIEVINTAAD
                     AGATTIGVEDSVGHMLPDEINTFLTDLIKSVGRKNIAFSFRAKNELGMAVANTLSAIK
                     AGVGQITCAINGTANDASLEETVSAIHTRQDAMNAQCDMLIAQLNRASVLLSGITDIK
                     NGKPGGVIGASLLISESDLLDKEMDFYSFRTFVEGLGYTLTEKDIERIYDMFKELCAK
                     KQQVYTKDIEAIIGREAVQVPRVYRLDRFVINSGNNITSTALIRLQYYDKPLEGVGMG
                     DGPIDAAFHVIEQIMGMNIELFDFNLKSVTEGKDALGAATVKIRYNGKIHVGRGLSTD
                     VIESSILAYINAVNKMLFEEGIQ"
     gene            1829..>3561
                     /locus_tag="PKJ65_03605"
     CDS             1829..>3561
                     /locus_tag="PKJ65_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ40958.1"
                     /translation="MKYAFSTASWKNLTWKEALAAAKDLGFSGIEAGACSVDAQPFSS
                     QYIMRTAEQLANLNLEISCFSSSCSLNDSENGKENIRKLKELIDLAAKMGTSYVRLLP
                     DDDMLPNDRATDRAVIMAISEILPYAEKKHVTLLIDTIGIYCDTDRLRKVLEHFASDN
                     LAALWNVHHPYRFRNESAEKTVQNLGAYIKYVHMKDSLMNDGAVKFTLMGEGDLPVDE
                     IIRALKSVNYDGYIAYEWITDQDSDLDEADIVFPHFMNYITSRFDENTKANKLYYNKA
                     KTGRFVWKKEQLIDCTFPQVLDRMVEEFPDQYAFRYTTLNYTRTYLQFRDDVDTFARS
                     LIALGVKKGDHVAVWATNIPEWYITFWATTKIGAVLVTVNTAYKIHEAEYLFRQSDTH
                     TIVMIEGHKDSHYAKIVKELCPELETSLPGQPLRIKRLPFLRNIITVDFSMQGCITWE
                     DAMDMHVKTPVEEVYRRANSIGKHDVCNMQYTSGTTGFPKGVMLTHYNIVNNGKSIGD
                     RMDLSTADRMMIQVPMFHCFGMVLAMTACVTHGSTMSPLPYFSPRPALNCINLEKITC
                     FHGVPTMFIAML"
BASE COUNT         1117 a          752 c          813 g          879 t
ORIGIN      
        1 cactccggta acgggaagcc cctttatttc cgcaggtacc gatattctgt tgctcttacc
       61 gtggtacttg gtaatgtgta tctctttccc gttgattatg tttatggaaa aaagattctc
      121 cttggtgtcc gcacaggaaa cggaaaatgc cgccacaaga accagcacaa ggacaaacat
      181 taccgctttt atatatctct tcatatcacc ttgtatgaca cggccggcaa ccggtcacac
      241 tcctccttaa tcaccatgtt caatatatat atcacaaaag tgagtgtatt gtcatcaaaa
      301 tctaaataat cggatttccc gtgaagggaa attatgtgaa aaaatagact tttttctact
      361 ccgatgatat aatccgttat taataatatt ctgaacgggg aggtgtcggt atgaaaaccg
      421 cgattttgat aaaggactgc acactgaaaa actgtgagac cgataaagct ttgaatataa
      481 gcttcaagga aaaactggaa attgccagac aattggaaaa catcaatacc gatataatcg
      541 agacgggttt tgcagacacg tcccagggag ctttcattgc ggtaaaaacc atagcatcca
      601 atctgaaaaa gagcatactc tgctgccccg ctcaggcgtt caaggcaagt atagataaga
      661 catatgaagc cataaaagga gcggtcaggc cgcgtataca catttcgctt ccggtatcct
      721 cggtaaatat ggaatatcag ttgcatatga agaaaaacca ggttgtggaa acggttaaac
      781 aaactatatc ttatgctaaa acccttttca gcgatgtgga gtttacggca ctggacgcca
      841 caagaagcga tatggatttt ctcatcgaag tgataaatac cgctgcggat gccggagcca
      901 caacaatagg ggtggaagat tcggtcggac atatgttgcc cgacgagata aatacctttt
      961 taaccgatct tataaaatcg gtcggcagga agaacatagc cttttctttc agggcaaaaa
     1021 atgaactggg aatggcggtt gccaatacct tatcggcaat aaaggcggga gtcggccaga
     1081 taacctgcgc cattaacggt acggcaaacg acgccagtct ggaagagacg gtatcggcta
     1141 tacataccag acaggatgca atgaatgcac aatgtgatat gctcatagcg cagcttaaca
     1201 gagcctcggt attgttgtcg ggaataaccg atattaaaaa cggaaaaccc ggaggcgtca
     1261 tcggtgcaag ccttctgata tccgaatcgg atcttctgga taaggaaatg gacttctaca
     1321 gcttccggac atttgtggaa gggctgggat atacactgac cgaaaaagat atcgagagaa
     1381 tatatgacat gttcaaagaa ctctgtgcca agaaacaaca ggtgtacacg aaggatatag
     1441 aggctatcat agggagagaa gccgttcagg ttcccagagt ataccgtctg gacaggtttg
     1501 ttataaacag cggaaacaac ataacttcca ctgctttgat acgacttcaa tattatgaca
     1561 agcctcttga aggagtgggt atgggagacg gtcccataga tgcggcgttt catgtaatag
     1621 agcagataat gggcatgaat atagagttgt ttgatttcaa tttaaaatcg gtgaccgaag
     1681 gaaaagacgc tttgggtgcc gctaccgtaa agatacgcta caacggaaaa atacatgtcg
     1741 gaagaggttt atcgaccgat gtaatagaat cctccatatt ggcgtatata aatgcggtaa
     1801 ataagatgct ttttgaggaa ggtatacaat gaagtatgca ttttccacgg caagttggaa
     1861 aaatcttaca tggaaagagg cgcttgccgc agcaaaggat ctgggctttt ccggtataga
     1921 agcaggagcc tgttcggttg atgcacagcc tttttcttcg cagtacataa tgagaacagc
     1981 tgaacagctg gcaaatctta atctggaaat atcctgtttc tcctcttcct gcagtcttaa
     2041 cgatagtgaa aacggaaaag aaaacatacg gaaactgaaa gaactgatag atcttgcggc
     2101 aaaaatgggc acttcatatg tgcgactgtt gcccgacgac gatatgttgc ctaacgacag
     2161 agctaccgac cgggcggtta tcatggcaat atcggaaata ttgccctatg ccgaaaaaaa
     2221 gcatgtcact ctgcttattg atacgatcgg aatttactgc gataccgaca gattaagaaa
     2281 agtactggag cattttgctt cggataatct tgcagcatta tggaatgtcc atcatcctta
     2341 cagattccgc aacgaatcgg cggagaaaac cgttcaaaac ttaggtgcat acataaagta
     2401 tgtccacatg aaagactcat tgatgaatga cggtgcagtg aaattcacac ttatgggtga
     2461 aggcgattta cccgtggatg aaataatccg ggcgctgaaa tcggtcaact atgacggtta
     2521 tatagcctat gaatggatta ccgatcagga ttccgatctg gacgaagcgg atatagtatt
     2581 tccccacttc atgaactata taacatccag gtttgatgaa aacacaaaag caaataagct
     2641 ctactataac aaagcaaaaa ccggccgttt cgtgtggaaa aaagagcagc tcatagactg
     2701 cacattcccg caggtcttag acaggatggt agaagagttt cccgaccagt atgctttccg
     2761 atataccact ctgaactata ccaggaccta tttgcaattt cgggatgatg tggacacatt
     2821 cgcccgctcc ctgatagcat tgggtgttaa aaaaggcgat catgttgccg tgtgggcaac
     2881 caacataccg gaatggtaca taactttctg ggcaaccacc aaaatagggg cggtgcttgt
     2941 tactgtaaat acggcataca agatacatga agcggaatat ctcttccgtc aatcggatac
     3001 gcatacgata gtaatgatag aaggacataa agattcgcat tacgcaaaga tagtaaagga
     3061 actctgtccc gaactggaaa cttcccttcc cggacaacct ttacgtatca agagacttcc
     3121 ctttctgagg aatatcatca ccgtggactt ttccatgcaa ggatgcataa catgggaaga
     3181 tgcgatggat atgcatgtaa aaaccccggt tgaggaagta tacagaagag ccaactccat
     3241 aggtaaacac gatgtctgta acatgcagta cacgtcgggt accacgggat tccccaaagg
     3301 cgtaatgctg acacactaca atatagtaaa taacggtaaa agtataggag accgtatgga
     3361 tttatccacg gccgacagaa tgatgataca ggttcccatg ttccactgtt tcggaatggt
     3421 cttggcaatg accgcctgcg tcactcacgg aagcacaatg tcgcctcttc cctacttctc
     3481 accccggccg gcactcaatt gtatcaattt agagaagata acctgtttcc acggagtccc
     3541 cactatgttc attgccatgc t
//
LOCUS       DAOPWC010000125         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26856_length_3546_cov_2.521627, whole genome shotgun sequence.
ACCESSION   DAOPWC010000125 DAOPWC010000000
VERSION     DAOPWC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26856_length_3546_cov_2.521627"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKJ65_03610"
     CDS             <1..809
                     /locus_tag="PKJ65_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNZ40959.1"
                     /translation="ILLVIKGMVVGAGAIIPGVSGGVLCVVFGIYQPMMALLAHPIKS
                     FKTYYKMFIPFLIGWLAGFLLIAKLVEMLFAASASIAIALFVGLIAGTLPSLFKEAGK
                     FGTDNKSRIGFALGLFLMFTLLLLLDNQSSSSITPNIWWFAFCGLIWGFSLIIPGLSS
                     SSILIFMGLYQPMTSGIAALDMAVILPLIAGLAVTVLLFARLVNHLFEKHHSIMHHTI
                     IGIVIASTLLIIPTEYPNWWSLIISAACFAAGFTASVLLNKLDKNSEKTA"
     gene            complement(837..>1509)
                     /locus_tag="PKJ65_03615"
     CDS             complement(837..>1509)
                     /locus_tag="PKJ65_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40960.1"
                     /translation="LPEKMKVIVFGPGGILENIVELVGYENMCIMIYEEPALFEKILD
                     EVASRLVRYYENCLKYDCVGAVMVNDDWGFKTATMLSPEDLRKYIFPYYKKIVEMAHK
                     SGRLTMLHSCGNLEEVMDDIIYDMKFDGKHSFEDNILPVEEGYRKYADKIAILGGIDL
                     DFIARESAENIRARCAALIKLTGYKGYALGTGNSIPDYIPFKNFAAMIGCVYPEFEEH
                     FSHLL"
     assembly_gap    1510..1519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1520..1851)
                     /locus_tag="PKJ65_03620"
     CDS             complement(<1520..1851)
                     /locus_tag="PKJ65_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40961.1"
                     /translation="MRQPDFDNIVKVLKRQTPDRPTLFEFYINNKTSEILSGRKSQSG
                     WDASWNYEMNIEAMSAAGYDYITLHGSDFSFPHFPKGQGYPIYDAASFSAYKFMDPDK
                     ANYKRLEE"
     gene            complement(1853..2605)
                     /locus_tag="PKJ65_03625"
     CDS             complement(1853..2605)
                     /locus_tag="PKJ65_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ40962.1"
                     /translation="MQIIDTHVHCGSIGTFDLKLKDVLRLMQNNCVDIAVISGIDNAE
                     FYDEHFERHSLDANTKLLEKIRGYDNLYMQYWIRPKFENMTEEIASFILNNRDKICGL
                     KIHPFTSSMCINDNRIKPYLEFADKNKLTVSVHTATGYNCECRYLAELCGNYPNADFV
                     AVHMDLMTDHTEAVKYVNEIPNLYGDVSWITYSDYKNLEVDEEKIVFGSDIPINMETE
                     YQFYKEYFINKEKEVKLLYNNAKRIFLDKRKF"
     gene            complement(2669..3502)
                     /locus_tag="PKJ65_03630"
     CDS             complement(2669..3502)
                     /locus_tag="PKJ65_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40963.1"
                     /translation="MKKNIRLLAIIIGICILSVCLFGGCNKTEKVTEKVYYLTGSFNG
                     YNVADEAYIMKKVSEESMLKQSMPDADLYYFTVSAESLTPDSVYGQHYYKVTAGNWDE
                     AWGTEVYDLQPAAVYYDADGNPTGLGSIYIPGDYKGELTVVFDAKAKKIYDNTMAKTF
                     EPRIYGDFNTAFGLKNDWDYSEEGSFAMKKNSAGKYEATLEVPAFKGTDEAGATMYVV
                     LGAKCYMAVSSEYDYISWGAAEQYIISGAGAQNVIKPDSSATYHITFDPQSKTITVVK
                     K"
BASE COUNT          935 a          804 c          612 g         1185 t
ORIGIN      
        1 ggatattgct tgtgataaaa ggaatggtag tgggagccgg cgccattatt cccggtgtaa
       61 gcggaggcgt tttgtgtgtg gttttcggaa tatatcagcc catgatggct ctgcttgcac
      121 atcctataaa gtcctttaaa acttactaca aaatgtttat tccttttctt ataggatggc
      181 ttgccggatt tttactcatt gcaaaacttg tggaaatgct gtttgcggca tccgcttcaa
      241 tagctatcgc actgttcgta ggattaatag cgggaacatt gccctccctg ttcaaggagg
      301 caggcaagtt cggcaccgac aataaatccc gtataggatt tgcgctgggc ttgttcttaa
      361 tgttcacctt attattgttg ctggataacc aatcgtcctc gtccataacc ccgaacatct
      421 ggtggtttgc tttttgcggt ctcatatggg gattcagcct tattatcccc ggcttgagtt
      481 cctcatccat cttaatattt atggggctgt atcagccgat gacttccggt atagccgctc
      541 tggatatggc ggtaatactt ccattgatag ccggtcttgc ggtaaccgtt cttttatttg
      601 ccagacttgt taatcatctg ttcgagaagc accacagtat aatgcatcac acaataatag
      661 gaatagttat tgcgtccacc ctccttataa tccctaccga atacccaaat tggtggagcc
      721 ttattataag tgcagcctgc tttgcagcag gctttacggc ttccgtatta ttgaacaaat
      781 tggataaaaa cagtgaaaag accgcttaat caaatatatt aaaatcagca ttcactttat
      841 aacaaatgcg aaaagtgttc ttcaaattcc ggatacacac accctatcat tgccgcaaaa
      901 tttttaaacg gtatataatc ggggatactg tttcccgttc ccagtgcata ccctttatat
      961 cccgtaagtt ttatcaatgc tgcacatctg gctctaatat tttccgccga ttcccttgca
     1021 ataaaatcca ggtcaattcc gcccagtatg gctattttat ctgcatattt cctgtatcct
     1081 tcttcaacag gcagaatgtt gtcttcaaat gaatgtttcc cgtcaaattt catatcataa
     1141 attatgtcat ccatcacttc ctctaagtta ccgcaggaat gcagcatggt taaacgaccg
     1201 cttttatggg ccatttccac tatctttttg tagtacggga atatgtattt tctcaaatct
     1261 tccggtgaca acatggtagc ggttttaaag ccccagtcgt catttaccat taccgcacct
     1321 acacagtcat attttaagca gttttcataa taacggacca atctgcttgc tacttcatca
     1381 agtatcttct caaagagtgc aggttcctca tatatcataa tacacatgtt ttcatagccg
     1441 acaagttcta caatattttc taaaatcccg ccgggaccga acactataac tttcattttt
     1501 tcaggcagtn nnnnnnnnnc actcttccag tcttttatag tttgctttat cggggtccat
     1561 gaatttatat gctgaaaaag atgccgcgtc atatatggga tacccctgcc ctttgggaaa
     1621 atgaggaaag gaaaaatcgc tgccgtgcaa agttatatag tcatatccgg cagcggacat
     1681 ggcttctata ttcatctcat aattccatga agcatcccat ccgctttgac ttttcctgcc
     1741 tgataatatt tccgaagtct tgttgtttat ataaaactca aacaaggtag gtctgtcggg
     1801 agtctgcctt tttaatactt ttacaatatt atcaaaatcc ggttgtctca tatcagaact
     1861 tcctcttatc caaaaagatt cttttggcat tattgtataa cagttttact tccttttctt
     1921 tattgatgaa atattctttg tagaattgat actcggtttc catgtttatg ggtatatcgc
     1981 ttccgaaaac aattttttct tcatccactt caaggttttt gtagtcgcta taagtaatcc
     2041 atgacacatc cccgtacaga ttgggtattt cattaacata ctttaccgct tcggtatgat
     2101 cggtcataag atccatatgt accgctacga aatccgcatt cggataattg ccgcataact
     2161 cggctaaata tcggcattcg caattatatc ccgttgcggt atgtaccgat acagtaagct
     2221 tgtttttatc cgcaaactca agataaggct ttattctatt atcatttata cacatacttg
     2281 atgtgaaagg atgaattttc agtccgcata ttttatccct attgttaagg ataaatgaag
     2341 ctatttcttc tgtcatgttt tcaaacttgg gtcttatcca gtactgcata tacagattgt
     2401 catatcccct tatcttttcc aacaacttag tgttggcatc caggctgtgt ctttcaaaat
     2461 gttcatcata aaattcggca ttatctatac cggaaataac agctatatcc acacagttat
     2521 tttgcataag ccgaagtaca tccttcaatt tcaaatcaaa agtgcctatg ctaccgcaat
     2581 gcacatgcgt atcaattatc tgcatattgt cctttctgtt cacttataaa gggcaggaca
     2641 ttactgccct ttttaagtta ctttgttact actttttgac aacagttatc gttttggact
     2701 gaggatcaaa agttatatgg taagtggctg aagaatcagg ttttatcaca ttctgtgctc
     2761 ctgctccgga tataatatac tgttccgcag caccccagga aatgtagtca tattcgctac
     2821 ttacagccat atagcatttg gctcctaaaa caacatacat cgttgctccc gcttcatcgg
     2881 tccctttgaa tgccggaact tccagtgtag cctcatattt accggcactg tttttcttca
     2941 tggcaaaaga gccttcctcc gaatagtccc aatcattttt taacccgaat gccgtgttaa
     3001 agtccccata tatcctgggt tcaaaggttt tagccatagt gttatcatat atcttcttgg
     3061 cttttgcatc aaacactacc gtcagttctc ctttgtaatc accgggtatg tatatggatc
     3121 ccaatcccgt cggatttccg tcagcatcat aatataccgc tgcgggttgc aaatcatata
     3181 cttcggttcc ccatgcttca tcccaattac ctgccgtcac tttgtagtag tgctgtccgt
     3241 acacggaatc gggagtcaaa ctttccgcac ttactgtaaa atagtaaaga tcagcatccg
     3301 gcatgctttg tttaagcata ctttcttcgc ttaccttctt cattatgtat gcttcgtccg
     3361 caacgttata tccgttaaaa ctacctgtaa ggtaatatac cttctcggtc actttctcgg
     3421 tcttattaca acctccgaat aaacatacgg acagaatgca tatgcctatg ataatggcaa
     3481 gcaatctaat gtttttcttc attttccata accacctttc tgatttttta tgcaaatcgc
     3541 aaaaga
//
LOCUS       DAOPWC010000126         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26897_length_3542_cov_5.078864, whole genome shotgun sequence.
ACCESSION   DAOPWC010000126 DAOPWC010000000
VERSION     DAOPWC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26897_length_3542_cov_5.078864"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ65_03635"
     CDS             complement(<1..204)
                     /locus_tag="PKJ65_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ40964.1"
                     /translation="MERNVVKNIALTGMFIALGIVLPFLTGQIPQIGSMLLPMHIPIM
                     LCGLVLGWKYGAIAGFITPLLRSL"
     gene            complement(293..1009)
                     /locus_tag="PKJ65_03640"
     CDS             complement(293..1009)
                     /locus_tag="PKJ65_03640"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNZ40965.1"
                     /translation="MTNREKVLKEFNNVPSGQLIKASNLYREKFSTVMSETAFTGTIS
                     RLYQNGEIYRISKGIYCKPRKTRFGSVKPSDKEIVNNLISDNNGVIVGYELYNSLGIT
                     TQIAKSLTVYSSIIDEKLKQIGNITVHKYNLDYNSSVKATIRIMEVLKNYNKIEDINY
                     SALIHTMKSYSKKYDEKAVETVIEVIGYPKRTVAFMHEMLNHYNVTNKLDNHLSALSD
                     YRIPEMKDIYEAAEKQPKSE"
     gene            complement(1012..2412)
                     /locus_tag="PKJ65_03645"
     CDS             complement(1012..2412)
                     /locus_tag="PKJ65_03645"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ40966.1"
                     /translation="MNLGKETETTEFKESTSERQKALESVAAIINKHGYGTLYFGVKD
                     NGDIKGQIVSDSTIKDLAEAIFRDIEPRIIPTIEVMEYDNKSVIKVGFSGTQVPYSAF
                     GKFLIRVGTQNRKMSRDELIKMIKKHEYSSDWEKEVKYQVEDIDDATLQAFYYEAVNC
                     GRLEMPVFNKKSLLSVLDVMKDDKVMNAAYALFGKGNPVSLKVACYATDEKITFLDLK
                     EFKGNIYNLISEAMLYIRKNIRWRVDIGARKRVEIPEIPIRAIREMVINAFAHANYQS
                     SPEIEINIHPGKITIFNPGCFPDDLTPNDFIEKDLSSIKRNPLILSILFRCKDVEKSG
                     TGYKRMNQLCEESDIEWNFENTAYGFYFEFIRPNVHADDRPNVRPDLISEGMTAYEAT
                     IYNAINENIKVQKAELAKAIGKGEKTVQRTLSSLIQRGYIKRIGNNQYGYWKIAKRQP
                     LSEKAAKKSSKHINDE"
     gene            complement(2562..2638)
                     /locus_tag="PKJ65_03650"
     tRNA            complement(2562..2638)
                     /locus_tag="PKJ65_03650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2602..2604),aa:Asn,seq:gtt)
     gene            complement(2654..2770)
                     /gene="rrf"
                     /locus_tag="PKJ65_03655"
     rRNA            complement(2654..2770)
                     /gene="rrf"
                     /locus_tag="PKJ65_03655"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2863..>3542)
                     /locus_tag="PKJ65_03660"
     rRNA            complement(2863..>3542)
                     /locus_tag="PKJ65_03660"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          921 a          875 c          617 g         1129 t
ORIGIN      
        1 aagggatcgt aacagcgggg ttataaagcc ggcaatagca ccgtacttcc atcccaatac
       61 caggccgcat aacataatag gtatatgcat gggcaacaac atacttccaa tctgcggaat
      121 ctgtcccgtc aaaaagggca atacaattcc caatgctata aacatacctg tcagcgcaat
      181 gtttttaact acatttcttt ccattttata taccccctaa gttatttaaa acaatatcgg
      241 tgtgcccatt atacaacata aagtaatata ttgctattat tttaacaata cattattcac
      301 tcttcggctg tttttctgca gcttcataaa tatccttcat ttcaggtata cggtaatccg
      361 acagagccga cagatggttg tccaatttat ttgttacatt atagtggttt aacatctcat
      421 gcataaatgc cactgttctc ttcgggtacc cgataacttc aataaccgtc tcaaccgcct
      481 tctcgtcgta tttctttgaa tatgacttca tagtgtgtat aagtgccgaa tagtttatat
      541 cttcaatctt attgtaattt ttgagaactt ccataatcct tatagtagct ttgacagagc
      601 tgttgtaatc aagattatac ttatgaacgg taatattacc tatttgtttc agcttttcat
      661 caattattga agaatacaca gtcaggctct ttgcaatctg tgtagttata cccaaagagt
      721 tatacaactc ataacctaca attacaccgt tgttgtcgga tataaggtta ttaacaatct
      781 ctttatcgga cggctttacc gatccgaacc gtgttttcct cggtttgcag tatatgcctt
      841 ttgaaatacg atatatttca ccgttttgat acaaacggct tattgtcccg gtaaaagccg
      901 tttcactcat aaccgttgaa aacttctcac gatacagatt gcttgcttta atcaactgtc
      961 cgctcggtac attattgaat tcttttaaaa ccttttctct gttggtcatt gtcattcatc
     1021 atttatatgt tttgaagact tttttgctgc tttttcggag agcggttgcc gtttggcgat
     1081 cttccaatag ccgtattggt tattccctat cctttttata tatccgcgtt gaatcaaaga
     1141 acttaatgtt ctttgcactg ttttttctcc ctttccgatt gctttagcaa gctcagcctt
     1201 ttgaaccttg atattttcat taatcgcatt gtatatggtc gcttcatatg cagtcattcc
     1261 ttcacttatt aaatcaggcc ggacattcgg ccggtcatct gcgtggacat tgggtcttat
     1321 aaactcaaaa tagaagccgt aagcggtatt ttcaaaattc cattcgatat ccgattcttc
     1381 acataattgg ttcatccttt tatatcccgt tcctgatttt tccacatcct tgcatctgaa
     1441 taaaatgctt agaatcaaag ggtttctttt aattgaagaa agatcctttt ctataaagtc
     1501 attaggagta agatcgtcgg gaaagcaacc gggattaaaa atagtaatct tacccggatg
     1561 aatattaatt tcaatttcag gactgctttg atagtttgca tgtgcaaaag catttattac
     1621 catctccctt atagctctga tggggatttc ggggatctcc accctttttc ttgcaccgat
     1681 atccactctc caacgaatgt ttttccttat ataaagcatc gcttcgctta tcaggttata
     1741 aatatttcct ttaaactcct ttaaatcaag aaatgttatc ttttcatctg ttgcataaca
     1801 ggcaacttta agagaaaccg gattcccctt tccgaacagt gcatatgcgg cattcatcac
     1861 cttatcatct ttcataacat caagaaccga caacaaagac ttcttattaa atacaggcat
     1921 ttccagtctt ccgcaattaa ccgcctcata gtaaaaagcc tgcaatgtag catcatcaat
     1981 atcttcaact tgatatttaa cttccttttc ccagtcggaa gagtattcat gtttttttat
     2041 cattttaata agttcatcgc ggctcatttt gcgattttgc gttcctaccc tgattaagaa
     2101 tttaccgaaa gcggaatagg gaacctgggt cccggaaaac ccgaccttta taaccgattt
     2161 gttgtcatat tccattactt caatagtcgg aataatcctg ggttctatat ctctgaaaat
     2221 agcctccgct aaatccttaa tagttgaatc gcttacaatt tgtcctttta tatcgccgtt
     2281 atctttaact ccgaaatata aagttccata tccatgctta tttataatcg cagcaacgga
     2341 ttccaaggct ttctgtcttt ccgatgtcga ttctttaaac tcggtagttt ccgtttcttt
     2401 tcccagattc ataaagcaac ttcctcctat ctcaaaatta attgtaccat aatattaaaa
     2461 aatgtccacc caaatgtcca cccaaatgtc ccggcacatg tccacccgga tgtcctggca
     2521 gcataaaaaa acgtctcctg cataatatac aaaagacgtc ttggctcccc ctgtccggct
     2581 cgaacggacg accctgcggt taacagccgc atgctctacc ttgctgagct aaggaggaaa
     2641 gtaaaaaaga gctgtccggc agctccctat tttcccgggc cgtctccggc caggtatctt
     2701 cggcgtaagc gggcttaact gccgtgttcg ggatgggtac gggtgtatcc tcgctgccat
     2761 taccaccgga catactttca aaaccgcata atgttcaaag cttcttaact ttcgttacaa
     2821 cttcaatgaa gtatcctgac tgtcagaaag tctcgcttcc tgtaggtcaa gccctcggcc
     2881 tattagtacc ggtccgctca acacatcact gtgcttacac tcccggccta tcaaccatgt
     2941 agtctacatg gggccttact ccattaactg gatgggatat ctaatcttga ggatggcttc
     3001 gcgcttagat gccttcagcg cttatccgaa aggctcttgg ctacccggct gtgccactgg
     3061 cgtgacaacc gatgcaccat aggagccccc atcccggtcc tctcgtacta gggacagacc
     3121 ctctcaaata tcctgcgccc acgacagata gggaccgaac tgtctcacga cgttctgaac
     3181 ccagctcgcg taccacttta atcggcgaac agccgaaccc ttggaagctt ctccacctcc
     3241 aggatgtgat gagccgacat cgaggtgcca aacttccccg tcgctgtgaa cgcttggggg
     3301 aaatcagcct gttatcccca gggtaacttt tatccgttga gcgacggcaa ttccactctc
     3361 ataccgccgg atcactaagt cctactttcg tacctgatcg acctgtttgt ctttcagtca
     3421 agctgccttc tgcctttata ctctgcgcac gatttccttc cgtgctgagg caaccttcga
     3481 acgcctccgt tactcttttg gaggcgaccg ccccagtcaa actgcccgcc tgacagtgtc
     3541 cc
//
LOCUS       DAOPWC010000127         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26975_length_3535_cov_3.443391, whole genome shotgun sequence.
ACCESSION   DAOPWC010000127 DAOPWC010000000
VERSION     DAOPWC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26975_length_3535_cov_3.443391"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKJ65_03665"
     CDS             <1..369
                     /locus_tag="PKJ65_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40967.1"
                     /translation="NEGIYKYNVETGEVTLEKAIKFDNHGHCEVRFYPDYIYLIHNRN
                     DDYRALRDQDLVLYIYNWDYEIIETVELDFINKGKRGNFITDVGNYIIFASDTDNKPD
                     YYIDKSEIGTDKFAFHKIEN"
     gene            710..895
                     /gene="rpmB"
                     /locus_tag="PKJ65_03670"
     CDS             710..895
                     /gene="rpmB"
                     /locus_tag="PKJ65_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNZ40968.1"
                     /translation="MAKCEICDKSTGFGNKVSHSERKTGRTFKPNIRKVKINDNGTVR
                     RANVCTGCLRSDKVQRA"
     gene            1052..1609
                     /gene="efp"
                     /locus_tag="PKJ65_03675"
     CDS             1052..1609
                     /gene="efp"
                     /locus_tag="PKJ65_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ40969.1"
                     /translation="MITAGDFRNGITFEMDGTVYQIIEFQHVKPGKGAAFVRAKIRNV
                     KSGSIVERTFNPSDKFELARMERKDMQYLYSDGDLFYFMDVETYDQIPINGETLGDTL
                     TFVKENEVVQILSYKGEIFGVEPPNFVVLEVTHTEPGFAGNTAQGATKPATVETGAQI
                     KVPLFVNIGDLVRIDTRTGEYMERA"
     gene            1609..2331
                     /locus_tag="PKJ65_03680"
     CDS             1609..2331
                     /locus_tag="PKJ65_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ40970.1"
                     /translation="MTIRVTIWNEYLHEKNNPEAIKIYPEGIHETLKKHLSSEKDFVI
                     RTATLEQAEHGLTEEVLENTDVLIWWGHMAHGKVQDEVVQRVADRVRRGMGAILLHSS
                     HASKLMKALTGCSGRLLWRENNEKQIMWNLKPFHPVTQGIGDYIIIDGEETYGEPFGI
                     PNPDELLFISWFSGGNVFRSGWIKNIENGKLFYFQPGHETFPSYNNQEVIRVIKNAVR
                     YLKPAGFTEHTKSIWLKEPVVE"
     gene            2331..3194
                     /locus_tag="PKJ65_03685"
     CDS             2331..3194
                     /locus_tag="PKJ65_03685"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNZ40971.1"
                     /translation="MLSPKPVSHTNMEEIRDYFKYADKHMSLAQFSTTYLWRHSTGLL
                     YDIQDDFLYLFEKNYNYAAPLPLGNGDIFKALEKVKEFKQSVCGNNLVYCIGEDKVKL
                     FEDKYQVSELNDFFEYVYLTEDLINLSGRKFHGKRNHLNQFVSNNEYMYERITASDIP
                     LVMNLMDQWSRKKEHSVNLDVEKKAIYEILNYDIGADYRAGAILTDNQVVAFAIGEQI
                     SDDMACVYFEKADTDIKGSYAVINNEFVKNEFSHLLYINRQEDLGISGLKKAKESYNP
                     VFKVKNYFMKL"
     gene            complement(3181..>3535)
                     /locus_tag="PKJ65_03690"
     CDS             complement(3181..>3535)
                     /locus_tag="PKJ65_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ40972.1"
                     /translation="INEEASNISSGQKQLITIARTIMADNPMLILDEATSNVDTRTEI
                     LIQKAMDNLMLNRTSFIIAHRLSTIKNADVIIVIDDGRIVEKGNHKELLAKQGFYHKL
                     YYSQFDRVAANSIVS"
BASE COUNT         1180 a          564 c          781 g         1010 t
ORIGIN      
        1 aatgaaggga tatataagta caatgttgaa acaggtgaag tcacattgga aaaggcaatt
       61 aaatttgata atcacggaca ttgcgaagtg agattctatc cggattacat atatctcata
      121 cataatcgca atgatgatta tcgagcactg cgtgatcagg acttggtttt gtatatatat
      181 aattgggatt atgagattat agaaaccgtt gagttggatt ttattaataa gggcaaaaga
      241 ggcaacttta ttaccgatgt cggcaattat attatatttg catccgatac ggataataaa
      301 ccggattact atatagataa atccgagata ggaacggata agtttgcgtt tcataagata
      361 gagaattagt tttttataaa atagtattga aagtgattca aatggaactt agcatataga
      421 atctttccaa atactatgta gagaaacttg caccggataa attaactata tcttaagaaa
      481 ggagcatatg cgatattagg tcccaacggt ggggtaaaaa gtacccttat gttcctttta
      541 acagaaatat atcgttatat cgtcaaagaa tgacactcat aaggcagaat tattgaaata
      601 ttatggataa caggcaggga acggggcaga aaacggagaa aaataatgct tgcttattaa
      661 ttagtcgtat tgtataattc ataagcgtgc tgtttaggag gtgagataaa tggctaaatg
      721 tgagatatgt gataaatcaa cagggttcgg taataaagtc agtcactctg agagaaaaac
      781 cggacgcact tttaaaccta atatcagaaa agtgaagatt aatgacaacg gaactgtcag
      841 aagagctaat gtatgcacgg gatgcttgcg ttccgataaa gtgcaaagag cataattttt
      901 taaaaaagag ttagctcttt ttttttgcac attttcagtt taattacttt acaaaggttc
      961 atcaagttac actatcggga tatgccggaa tattgacacc attccggtga tagagtaaaa
     1021 tcagttaaga tgcttattat ggaggaaaat tatgattaca gcaggtgatt tcagaaacgg
     1081 aattacattt gaaatggatg gtacggtata tcagataata gaatttcaac atgtcaaacc
     1141 gggtaaaggt gcggcatttg taagagcaaa aatacgcaat gtaaaatcgg gatccatagt
     1201 ggaaagaaca ttcaatccgt cggacaaatt cgaacttgcc cgtatggaga gaaaggatat
     1261 gcaatacctt tattccgatg gtgatctttt ctattttatg gatgtggaaa cttatgatca
     1321 gattcccatt aacggcgaaa ctctgggcga tacactgaca tttgtcaaag agaatgaagt
     1381 tgtgcagata ttgtcataca aaggcgagat attcggtgtg gaacctccta atttcgtagt
     1441 tctggaagtt acgcataccg aacccggttt tgcgggaaat accgcacagg gcgctacaaa
     1501 gccggctact gtggagaccg gtgctcagat taaagtaccg ttgtttgtaa acatagggga
     1561 cctggtaaga atcgataccc gtaccggtga gtatatggag agagcataat gactattcgt
     1621 gttaccattt ggaatgaata tttacatgaa aaaaataatc cggaagctat aaagatttat
     1681 ccggaaggga tacatgagac cttaaagaag catttatctt ccgagaagga ttttgtaata
     1741 cgcaccgcta ccttggagca ggcggaacac ggacttaccg aagaggtact ggaaaatacc
     1801 gatgttctta tctggtgggg acatatggca cacggaaaag ttcaggatga ggttgtgcag
     1861 agagttgccg acagggtaag aaggggaatg ggtgcaatat tgcttcactc atcacacgca
     1921 tcaaagctta tgaaagctct tacaggctgt tcgggcagat tattgtggcg tgaaaacaat
     1981 gaaaaacaga ttatgtggaa cttaaaaccc tttcatcccg ttacgcaagg tataggcgac
     2041 tatattatta tagacgggga agaaacctac ggagaaccgt tcggtattcc taatcccgat
     2101 gaattgctgt ttatatcgtg gttttccggc ggcaatgtat tccgttcggg ttggataaag
     2161 aacatagaga acggtaaact attctatttt caaccgggac atgagacatt cccgtcatac
     2221 aataatcagg aagttataag agttattaag aatgctgtaa gatatcttaa acccgcagga
     2281 tttactgaac atacaaaaag tatttggctt aaggaacctg ttgtcgagta atgctcagtc
     2341 ctaaacccgt ttcacatacc aatatggagg aaataagaga ctactttaaa tatgccgata
     2401 agcatatgtc attagcgcag ttttccacaa catatctttg gcgtcacagt accggccttc
     2461 tgtatgatat acaggacgat tttttatact tgtttgaaaa gaactataat tatgcagcac
     2521 cgctcccttt aggtaacggc gatattttca aagctctgga aaaagtcaaa gagttcaagc
     2581 aatcggtatg cggaaacaat cttgtttact gcatagggga agataaggta aaactctttg
     2641 aagataaata tcaagtgagt gaacttaacg atttcttcga gtatgtctat ttgaccgaag
     2701 atttgataaa tctttcgggg agaaagtttc acggaaaaag aaatcatctc aatcagtttg
     2761 tcagtaacaa cgaatatatg tatgaaagaa taaccgcttc ggacataccg cttgttatga
     2821 atctaatgga tcagtggagc aggaaaaaag aacattcggt caatctggat gtggaaaaga
     2881 aggccatata tgagattctt aattatgata taggtgcaga ctacagagcg ggagcaattt
     2941 taaccgataa tcaggtggtt gcatttgcca taggcgaaca gatatccgat gatatggcat
     3001 gtgtatattt cgagaaagcg gatacagata ttaaggggtc atatgcggta atcaataatg
     3061 agtttgttaa aaatgagttt tcccatcttc tatatataaa cagacaggaa gacctcggga
     3121 taagcggtct taagaaagct aaggaaagtt ataatcccgt ttttaaagta aaaaactact
     3181 ttatgaaact atagaattgg cggcaactct gtcaaactgg ctgtaataca acttgtgata
     3241 gaaaccttgt ttggcaagaa gttccttatg gttacctttt tccacaattc ttccgtcatc
     3301 tattacaata ataacatccg cattttttat agtagacaat ctgtgagcta tgataaacga
     3361 ggtgcggtta agcatcagat tatccatggc tttctgaatg agtatctcgg ttcttgtgtc
     3421 gacattggat gttgcttcat ccagaataag catggggtta tccgccataa tggtacgggc
     3481 tattgtaatt aattgcttct gaccggagga aatattgctg gcttcttcat tgata
//
LOCUS       DAOPWC010000128         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_26981_length_3534_cov_3.873239, whole genome shotgun sequence.
ACCESSION   DAOPWC010000128 DAOPWC010000000
VERSION     DAOPWC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26981_length_3534_cov_3.873239"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..104
                     /locus_tag="PKJ65_03695"
     tRNA            31..104
                     /locus_tag="PKJ65_03695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Gly,seq:ccc)
     gene            complement(219..1346)
                     /locus_tag="PKJ65_03700"
     CDS             complement(219..1346)
                     /locus_tag="PKJ65_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNZ40973.1"
                     /translation="MVTKKKYMTKLMENEKYLTEQIITYIGNKRSLLDFIEEAVEIVR
                     KELNKNKLDIADLFSGSGIVARYLKKYSNKIITNDLENYSYTLNKCYLSNENEINTDE
                     IVSYYNRLKDRLEEKHISGFITKLYAPEDDGDIKKGERVFYTNKNARYIDTCRQTIAT
                     FPVDIQHFFIAPLLTEASIKNNTGGVFKGFYKNRETGIGQFGGTGKNALSRIKADIDI
                     PFPVFSNFNAEVINYQCDANALIDTLEEVDLLYLDPPYNQHPYGSNYFMLNLINDYIE
                     PENISEVSGIPEDWNRSGYNKKNKSLNILYDLCKKAKAKYILISFNSEGFITKEQMEK
                     MLKKTGTVRTLGKKYNTYRASRNLNNRNTYVHEYLFLVKKS"
     gene            1584..2600
                     /locus_tag="PKJ65_03705"
     CDS             1584..2600
                     /locus_tag="PKJ65_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40974.1"
                     /translation="MNMKKWLEDIKKSQVKKALPVLSFPSIQLLNISVRELISSSDYQ
                     AKGMKAVADATDALAAVSLMDLSVEAECFGSHVVASDDEVPTVTGHVVETMQQAEDLP
                     VPEVGSKRTGLYIEAIKKAKQLITDRPVLAGVIGPFSLAGRLLDVTEAMVLCYEDPDL
                     VHVVLEKCTEFIINYIEAYKKAGADGIVLAEPLGGLLSPSLADEFSSAYVKKIVESTQ
                     TDDFAIIYHNCGDNVIQMIEGILDTGAAAFHFGNSIDMSVMMTKIPKDIIAMGNVDPA
                     AQIRNGTPRSVREATLAIMDKCSSYDNFIISSGCDIPPMAPWENINAFFDAVEEYNKI
                     HGRI"
     gene            2634..>3534
                     /locus_tag="PKJ65_03710"
     CDS             2634..>3534
                     /locus_tag="PKJ65_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ40975.1"
                     /translation="MDIKGKRIYDTMKKMNFIRLSTTEGEKSGAKVITDEIKAMGLEP
                     VFEDFKVPCYEIVNVKLEITEPKYMLVEAKGYGYSGNAAKDGITADFAYVEAAEDIDL
                     IDAEGKIVLVSNMGYEIYERLAKAKVAGFIAPSGGYFDDPKKTDLDERMLRKGHITYG
                     QIPGVSIRMKDAVKILKTNPKKAKITLEQKEEEGDSGNIIVEAKGTKHPDEVIVYTAH
                     YDSVVFSKGMYDNASGSAILLELLRYFLKNSPLRTVRFIWCGSEERGLFGSKDYVKRH
                     EEQLKDIKLCINVDLAGPIMGRDT"
BASE COUNT         1082 a          620 c          775 g         1057 t
ORIGIN      
        1 taaaacataa aaagataaaa ttccctttaa gcaggtatag ctcaatggca gagtcccggc
       61 ttcccaagcc ggttatgcgg gttcgatccc cgttacctgc tccatttaac ttctatttaa
      121 tgtttatcag gacaaatgtc caaataaata tgtaattaag cgataagaaa caggttgtcc
      181 gatactaaga gcgatcagga agtatctgta cggtgttctt atgacttctt taccaaaaac
      241 agatattcat gcacataggt gttgcggtta ttcaaatttc tggaagctct gtaagtgttg
      301 tacttttttc ccagtgttcg gacggttcct gtttttttca acattttttc catttgttct
      361 ttggttataa agccttcgga gttaaaggaa atgagtatgt attttgcttt tgcttttttg
      421 caaaggtcat acagtatatt taaagattta ttctttttgt tatatcccga cctgttccag
      481 tcttcgggaa taccggaaac ctcactgata ttctcaggct ctatatagtc attaatcaag
      541 ttaagcatga agtaattgga accgtaagga tgctgattat acggcgggtc cagatacaat
      601 aagtccacct cttccaatgt atcaatcaga gcattggcat cacactggta gtttataact
      661 tccgcattaa aattactgaa aaccgggaag ggaatatcta tatccgcttt tattctggat
      721 aatgcattct ttccggtacc tccgaactgt ccgatgcctg tttccctgtt tttgtaaaac
      781 cctttaaaaa ctcctccggt attgtttttt attgaagctt cggtcaataa aggcgctatg
      841 aaaaagtgtt gaatatcgac gggaaaggtt gctattgtct gtctgcaggt atcgatatat
      901 cttgcgtttt tattggtata gaaaactctt tctccctttt ttatatcacc gtcatcttcg
      961 ggagcataca gttttgtaat aaacccgctt atatgctttt cttcaagtct atctttcagc
     1021 ctgttgtaat aacttactat ttcatctgta ttaatttcgt tttcattgct taaataacac
     1081 ttattcaacg tatatgaata attctccaaa tcattggtaa taattttatt agaatatttt
     1141 tttaagtatc gcgcaacaat tcccgaaccg gagaacaggt ccgctatatc caacttgttt
     1201 ttattcaatt cttttcttac tatttcaacc gcttcttcga tgaaatccag cagggaacgt
     1261 ttgttaccta tataagtaat tatctgttcg gttaaatatt tttcgttttc catcagtttt
     1321 gtcatatatt ttttctttgt caccatatcg gaagaattgt cgctttgtta tttaacacta
     1381 ttaaatcata tcattttttt acacaatatt ccgttaattt tacaaacaga aaacatcaga
     1441 gtacaaagtt tggtttcgtg ataaaaaaga aaaagagaaa agataagata tttacacata
     1501 tagtgcggca cttatctttt tttgtggtag aataagaaac ggtgcaaaat acaatttatc
     1561 atatgtcatg tggaggtgtt ttgatgaata tgaagaaatg gctggaagat attaagaaaa
     1621 gtcaggtgaa aaaggctttg ccggtattat ctttcccctc aatccagttg ttgaacataa
     1681 gtgtaaggga gctcatatcc agttccgatt atcaggcaaa aggcatgaag gccgttgcgg
     1741 atgcgaccga tgcgcttgcg gctgtaagcc ttatggacct ttcggtggaa gcagaatgtt
     1801 tcggctcaca tgtggttgca tcggatgacg aagtgcctac cgtaaccggt catgtagtgg
     1861 aaaccatgca gcaggcggaa gatttaccgg tacccgaagt cggaagtaaa aggaccggtt
     1921 tatacataga agcaattaaa aaagcaaaac agttgataac cgacagaccg gtacttgccg
     1981 gtgtaatagg acctttctcc ttggcgggaa ggcttttaga tgtcaccgaa gcgatggtac
     2041 tttgttatga ggatccggac ctggttcatg ttgtgctgga aaaatgtacc gagttcatta
     2101 tcaactacat tgaagcatac aaaaaagcgg gggcggacgg tatagtgctt gcggagcctt
     2161 taggaggtct tttgtcgccg tcattggcgg atgagttctc atcggcttat gtcaagaaga
     2221 tagtggaaag tacacagacc gatgattttg caatcatcta ccacaattgc ggagataatg
     2281 taattcagat gatagaaggt atactggata ccggtgcagc ggcattccat ttcggaaact
     2341 ccatcgatat gtcggttatg atgaccaaaa tacctaaaga tataattgca atgggtaatg
     2401 tcgacccggc agctcagata agaaacggca caccccgatc ggtcagagag gcgacactcg
     2461 ctatcatgga taaatgttca tcatatgaca acttcataat atccagcgga tgtgatatac
     2521 ctccgatggc tccgtgggag aatattaatg cgttctttga tgcggtagag gaatacaata
     2581 aaatacacgg aagaatttaa agaagaaaag gtaaagcagt taaggaaaaa ggtatggata
     2641 taaaaggtaa aagaatatat gacacgatga agaaaatgaa ctttatccgg ctttccacca
     2701 ccgagggtga gaaaagcgga gcaaaggtca taaccgatga gataaaggca atgggtttgg
     2761 agccggtttt tgaggatttt aaagttcctt gctatgagat agtaaatgtg aaactggaga
     2821 ttaccgaacc taaatatatg cttgtggaag caaaaggtta cggatacagc ggtaacgccg
     2881 caaaagacgg tatcacggcg gattttgcct atgtggaagc ggccgaagac atagacttaa
     2941 tagatgccga aggtaagatt gtattggtct ccaatatggg ctatgagata tatgaacgcc
     3001 ttgccaaggc aaaagtagcg ggatttattg caccgagcgg aggctatttt gacgatccga
     3061 aaaagacaga tctggatgag cggatgttga gaaaaggcca tattacttac ggtcagatac
     3121 cgggagtatc cattagaatg aaagatgctg taaagatatt aaagaccaat ccgaaaaaag
     3181 caaaaattac tttagagcaa aaagaagaag aaggcgattc gggtaatata attgtggagg
     3241 caaaaggcac aaaacatccc gatgaagtaa tagtctacac ggctcattac gattcggttg
     3301 tcttcagtaa aggtatgtac gataacgcat cgggttcggc aatacttctg gaactgttaa
     3361 gatatttcct taaaaattct cctctgagaa cggtcagatt tatctggtgc ggttcggaag
     3421 agagaggtct tttcggaagt aaggattatg taaaacgaca tgaggaacaa ttgaaggata
     3481 ttaagttgtg tattaatgtg gatcttgccg gtccgataat ggggagagat acgg
//
LOCUS       DAOPWC010000129         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_27000_length_3532_cov_2.403509, whole genome shotgun sequence.
ACCESSION   DAOPWC010000129 DAOPWC010000000
VERSION     DAOPWC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27000_length_3532_cov_2.403509"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKJ65_03715"
     CDS             <1..873
                     /locus_tag="PKJ65_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ40976.1"
                     /translation="PRFNAASYAKLLKKKQPNYIAGVPTLFEALLRNPYMDNVNLSCL
                     EGVFSGGDSLSIELKRKLDKFLKEHGAHVQVREGYGTTECVTASCLTPKDFYKEGSIG
                     IPYPDTYYKIVKVGTTEQVPYNEEGEICIHGPSVMLGYCNNEKETADTLRKHDDGYIW
                     LHTGDLGKMDEDGFVYFSQRIKRMIITSGYNVYPSRLENILDAHEAVYMSCVIGVKDP
                     YKIQKVKAFVVLKAGFEPNDITKQAILEYCKKHIAKYALPYDIEFRDDLPKTLVGKVA
                     YRELEKEEMNKMGA"
     gene            complement(918..2246)
                     /locus_tag="PKJ65_03720"
     CDS             complement(918..2246)
                     /locus_tag="PKJ65_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ40977.1"
                     /translation="MITITRMEPIDKGLVFEYRCSSEYQLLMTVKPLTTSMPLIQKKL
                     DKSETGVTVSNLTNNLDYFISLTSVDNSGRIKEPSCTRLFTPYPNKRKFKTVNYIHKD
                     DYFYYPSGRSTCSPSILKLDENTYICSHDVFWQYTAQNLTLIFKSKDKGETWEYLSQL
                     SPCFWGSLFLINGIVYMYGISTEYGDIMLYKSYDKGATWPQSVCLFTKCQDDVKGFHK
                     APMPAIISDGRLWLNVEYGTWKHKFAIHALSCCIDDDIMNPANWTITEPLYIAKNDEG
                     IIAKEDPVCIEGNLVQGFDGKLYNILRCNLIQTKAAVLCEVNKQDISAPLRFIKAVDL
                     PVSHSKFVVRTHSDGYHYAMGNRIDENCKNIRQILSVYRSKNLIDWEFCKDIADVLGT
                     ADRKKAALQYPDWFIEGKDIVYVSRTAINKPYNFHNANNITFHRYKDFFA"
     gene            2476..>3532
                     /locus_tag="PKJ65_03725"
     CDS             2476..>3532
                     /locus_tag="PKJ65_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNZ40978.1"
                     /translation="MSTGMNFWDTGMWSFIITLTILLTSMMVANFLRRVIKPLRRSLI
                     PSSVLGGFLALFADFIFKKITGNSMFNPVMLEALTYHGLGLGFAAVALKTTERKIVGD
                     SKKDIMNYGLTTVSAYVLQGAIGIAIMLILGLFLSNTFPAAGLLLPMGYGQGPGQAYN
                     WGHTYEVLWGFEHGTSFGLTLAATGFISASVGGIFYLERLKKKGLVKTTFEDEIVASE
                     EDEENKPAKGEIPLSESMDKLTVQVALVLIAYALAYLFMWGINRIIETGTFGTFGTNT
                     LQPLIWGFNFLISTVFAVLVKTFLRFLKKKNIMKREYTSNFMQNRISGFMFDIMVVAS
                     IAAIKLSAFQHRSFIIPI"
BASE COUNT         1036 a          651 c          769 g         1076 t
ORIGIN      
        1 cctcgtttca atgcagcttc ctatgcaaaa ctcttaaaaa agaagcaacc caattatatt
       61 gccggtgtac ccactctgtt tgaagctctc ttaagaaatc cgtacatgga taatgtaaat
      121 ttaagctgtc ttgaaggagt attctccgga ggagattctt tatctattga gcttaagaga
      181 aagttggaca agtttttaaa agagcacggc gcacatgtgc aggtcaggga agggtacgga
      241 actaccgaat gtgtcacggc aagctgtctt actcccaagg atttctacaa agagggaagt
      301 ataggaattc cgtatcccga cacatattat aaaatagtca aagtagggac aacagagcaa
      361 gttccgtaca acgaagaagg ggagatatgt attcacgggc cgtcggtaat gctggggtat
      421 tgcaacaatg aaaaagagac tgcggataca ttgagaaaac acgatgacgg ttatatatgg
      481 cttcataccg gtgatttggg caagatggat gaagacggat tcgtgtattt ttctcaaaga
      541 ataaagagaa tgattattac ttcgggatat aatgtttatc cgtcccggtt ggagaacata
      601 cttgatgccc atgaggcggt atacatgtct tgtgtaatag gtgtcaagga tccgtacaaa
      661 atccagaaag taaaggcatt tgtggtattg aaagcgggat ttgaaccgaa tgatataaca
      721 aaacaggcaa tattggagta ctgcaagaag catattgcca aatacgctct gccgtatgac
      781 atagagttca gagacgattt acccaaaaca cttgtgggaa aagtcgctta cagggaactg
      841 gaaaaagaag agatgaacaa aatgggagct taagactcct ttttgtttgt tgtcggttgc
      901 aaaacttatt tgtcggatta tgcaaagaaa tccttatatc tgtggaaagt tatgttattt
      961 gcgttatgga agttatacgg tttattgatt gcggttctgc ttacataaac tatgtctttg
     1021 ccttcgataa accagtcggg atattgcaat gcggcttttt ttctgtccgc tgtgcctaaa
     1081 acatcggcaa tgtccttaca gaattcccag tctataaggt ttttcgagcg atatacgctt
     1141 agtatctgcc ttatattttt acaattttca tcaattctgt ttcccattgc gtaatgatat
     1201 ccgtcgctat gggtcctgac aacaaatttg ctgtgcgaaa cgggaaggtc caccgctttt
     1261 ataaacctca acggtgccga tatgtcctgt ttattcactt cgcataaaac cgccgctttg
     1321 gtctgtatta aattgcatcg gagaatgtta taaagtttac cgtcaaaacc ctgcacaaga
     1381 ttgccctcta tgcatacggg atcttccttt gcaattattc cttcatcatt ttttgcaata
     1441 tataaaggtt ctgtgattgt ccagttggca gggttcatta tatcgtcatc aatacagcag
     1501 gaaagggcat gtattgcaaa tttgtgtttc caggttccgt attcaacatt cagccacagt
     1561 ctgccgtctg aaataatcgc aggcatgggg gctttgtgaa atcccttgac atcatcttga
     1621 cactttgtaa acagacatac cgattgcggc caggtggcac ctttatcata agacttatac
     1681 agcattatat ccccgtattc ggtgcttata ccatacatat atacaattcc gtttatcaaa
     1741 aagagactgc cccagaagca gggagacaac tgacttaagt attcccaagt ttctcctttg
     1801 tcttttgact tgaaaatcag tgtgagattc tgagcggtat attgccagaa gacatcatgt
     1861 gaacatatat aggtgttttc atcaagttta agtatgctcg gtgagcaggt agatcttcct
     1921 gaaggatagt aaaagtagtc atctttgtgt atatagttta ccgttttgaa tttcctttta
     1981 ttcggatagg gggtaaaaag cctggtacac gacggttctt tgattcttcc cgaattgtca
     2041 actgaagtga ggctgataaa gtagtccaga ttgtttgtga gattgctaac agttacccct
     2101 gtttcggatt tgtccagttt cttttgtatc aagggcatac ttgtcgtcaa cggttttacg
     2161 gtcattaaaa gctgatattc cgaactgcat ctgtattcga ataccagtcc tttatctatc
     2221 ggctccattc tggttattgt aatcatgctg tcctccgtta tgctcacctt aatcttacca
     2281 aataagtttg tcttattcca atattttacc gggaaaggat taggtaacaa agaggtaaaa
     2341 aagaaatcgg aagaagaaaa aaaacagaaa atatccgggt atttaaccaa tattttatat
     2401 tgcaattggc agttcatgtg ttatactgaa ataagttatg actagctcat aaaaactttc
     2461 aaaggagagc atattatgtc tacaggaatg aacttttggg ataccggaat gtggtcgttt
     2521 ataatcacgc ttacgatttt gcttaccagt atgatggtgg caaatttttt aagaagagtt
     2581 ataaagccgc ttagaagatc attaataccc agctcggtgt tgggaggttt tctcgcactg
     2641 tttgccgatt tcatattcaa gaaaattaca ggtaattcca tgttcaatcc tgtaatgctg
     2701 gaagcattga cttatcacgg tttgggcttg gggtttgcgg cagtggcatt aaaaaccacc
     2761 gaacgtaaaa ttgtcggtga cagtaagaag gatataatga attacggatt gacaacagta
     2821 tccgcttatg tgcttcaggg agcaatcggt atagccatta tgttgatttt aggattattc
     2881 ttgtccaaca cattcccggc tgcaggactg cttttaccta tgggatacgg gcagggaccg
     2941 ggtcaggcat ataactgggg acatacctat gaggtcctgt ggggatttga gcacggtaca
     3001 agtttcggtc ttactcttgc tgctaccggc tttatatcag ccagtgtagg aggtattttt
     3061 tacttggaaa gattaaagaa gaaagggttg gtcaaaacca catttgaaga tgagatagtt
     3121 gcatcggaag aagatgaaga aaacaaaccg gctaaaggag agataccgtt atccgaatcc
     3181 atggataagc ttaccgtgca ggttgcttta gtgcttattg cctatgctct tgcatatctc
     3241 tttatgtggg gaataaatag aattatagaa accggcacgt tcggcacttt cggtacaaat
     3301 acattgcaac ctctcatatg ggggttcaat ttccttatat ccacggtatt tgcagtactt
     3361 gttaaaactt tcctcagatt tcttaaaaag aaaaacataa tgaagaggga atacacatcc
     3421 aatttcatgc agaacaggat atcggggttt atgttcgata taatggtggt ggcttctata
     3481 gccgcaataa agctttccgc tttccagcac cgttcattca taatacccat ca
//
LOCUS       DAOPWC010000130         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_27382_length_3497_cov_3.751307, whole genome shotgun sequence.
ACCESSION   DAOPWC010000130 DAOPWC010000000
VERSION     DAOPWC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27382_length_3497_cov_3.751307"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1005
                     /locus_tag="PKJ65_03730"
     CDS             130..1005
                     /locus_tag="PKJ65_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNZ40979.1"
                     /translation="MAYSMTGYGKSLYENQLISMRVEIKSINSKFLDLNLRLPKSISF
                     AEEKVRNLLRSRITRGKIDCYVTVHDMHASFTDIKFDEKVADAYFGFLKSIKRRYNLI
                     AGITPVQIASFPGVINTTESDEDYDKLTDCILEAFNIAIDNILVAKKKEGEILCADCE
                     LKLKEFENLVNNIQKVSADLPAQYKQKLEKRIAELLEDVKTDETRIAQEVAFFADRCC
                     VDEEITRLRSHIKQFSNNLHNETSGKKLDFVLQEMLRETNTIASKANSLDVINNVLEM
                     KCIIEKIREQIQNLE"
     gene            1002..1277
                     /locus_tag="PKJ65_03735"
     CDS             1002..1277
                     /locus_tag="PKJ65_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNZ40980.1"
                     /translation="MEMKFLNIGFGNMVSIERIVAILSPESAPVKRIIQEARDISVLI
                     DATYGRRTRAVIITDSEHVILSPLQPETLERRLSGGVVPDDISEESN"
     gene            1298..1552
                     /locus_tag="PKJ65_03740"
     CDS             1298..1552
                     /locus_tag="PKJ65_03740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNZ40981.1"
                     /translation="MINKPSVEQLEKKIPVRFVIVNALAKRSREIAAGARVRIETNEK
                     NPITVAAMEIDSGAVRVVDEPVAEELQEEEIITESESVDE"
     gene            1545..2936
                     /locus_tag="PKJ65_03745"
     CDS             1545..2936
                     /locus_tag="PKJ65_03745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ40982.1"
                     /translation="MSKEQVTMDKLVSLAKRRGFVYPGSEIYDGLANAWDYGPLGVEL
                     KNNIKAAWWKKFITDNEYNVGLDCAILMNPNVWVASGHVGSFVDPLTDCKNCKTRHRA
                     DELIKEYNKSKSIEMIVDGLDNATLTTYIKEKGIPCPNCGKQDFTEVKQFNCMFKTYQ
                     GVNEDTAAQIYLRPETAQGIFVNFKNVQRTTRRKIPFGIGQIGKSFRNEITPGNFIFR
                     TREFEQMELEFFCKPGTDLEWFEYWRTFCHNWLLSLGLNKDNIRLRDHSEKELSFYSK
                     ATTDFEYLFPFGWGELWGIADRTDYDLKQHSEFSKQDLSYFDPITEEKYIPYCIEPSV
                     GVERLLLAILTDAYDEELLEKDDSRTVLRLHPFLAPVKAAILPLAKRLGDDAFKVYEM
                     IRKKWPAEYDDTQSIGKRYRRQDEIGTPFCFTYDFDSLEDNCVTVRYRDSMKQDRIRI
                     DSIMTFLEENIKL"
     gene            2949..>3497
                     /locus_tag="PKJ65_03750"
     CDS             2949..>3497
                     /locus_tag="PKJ65_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ40983.1"
                     /translation="MTKYVYLFEEGNAKMRNLLGGKGANLAEMTTLGLPVPRGFVVTT
                     EACLRYYEDGEKIGKDIEEEILKMLKVTEKAVGKKFGDPENPFLVSVRSGSRASMPGM
                     MDTVLNLGLNDKVVEGLAKLTNNPRFAYDSYRRFVMMFSDVVIGLSKSKFERILDEIK
                     KEKGIVNDTDLTADDMKFVLKKF"
BASE COUNT         1206 a          568 c          814 g          909 t
ORIGIN      
        1 ttcaatttgg gatttctctt ttattctatc aatccccaaa caagtttaca ctatccatgc
       61 agcggaaaat gttgcatgtg cttgttgtgt atgttacaat atttaagatt aatataagga
      121 tgcgtaagta tggcatacag catgacagga tacgggaaaa gcctgtatga aaatcagttg
      181 atatcgatga gagtggagat taagagtata aacagcaaat ttcttgattt aaacttacga
      241 ttgcctaaga gtatatcttt tgccgaagaa aaagtacgta accttttaag aagcagaata
      301 acaagaggga agatagattg ttatgttacc gtacatgaca tgcatgcatc ttttacggat
      361 ataaaattcg atgaaaaggt tgccgatgct tatttcggtt tcttaaaatc cataaagagg
      421 agatataacc tcattgccgg tataactccg gtacagattg catcttttcc gggagtaata
      481 aatacaaccg aaagtgatga agactacgac aaacttacag actgcatatt ggaagccttt
      541 aatattgcaa ttgacaacat tcttgttgcc aagaaaaaag aaggcgaaat actgtgtgcc
      601 gattgtgagt taaaacttaa agagtttgaa aatctggtaa ataatataca gaaagtatct
      661 gccgatcttc cggctcagta taagcaaaag ctggagaaga gaattgccga acttcttgag
      721 gacgtaaaaa ccgatgaaac cagaatagcg caggaagttg cattttttgc tgacagatgc
      781 tgtgtcgatg aggaaattac gcgtcttaga agccatatta agcagttttc caataatctg
      841 cacaatgaaa catccggtaa aaagctggac tttgtactgc aggaaatgtt aagagaaaca
      901 aatacaatag cttcaaaagc aaacagcctc gacgttataa acaatgtgtt ggaaatgaaa
      961 tgtattatag agaaaataag ggagcagatt caaaatctgg aatagaaatg aagtttttaa
     1021 acatcggatt cggtaatatg gtttcaatag aaagaatagt tgctatacta agccccgagt
     1081 cggcgcctgt aaaaagaata atccaggaag cgcgtgacat ttcggtactg attgatgcaa
     1141 catacggcag aagaaccaga gccgtaatca ttacggacag cgaacatgtt atattatcac
     1201 cccttcagcc ggagacattg gaaaggcgat taagcggagg agttgttccg gatgatattt
     1261 ccgaagagag taattagttg acataaggag attggaaatg ataaacaaac cgtcagttga
     1321 acaattggaa aagaaaatac ctgtaagatt tgtaatagtt aatgcattgg caaagcgttc
     1381 cagagaaata gcggcagggg caagagtgag gattgaaaca aacgagaaaa atcctattac
     1441 cgtagctgca atggaaatag acagcggtgc tgtaagagtg gttgatgaac ctgttgcgga
     1501 agaattacag gaagaagaaa taattacgga aagtgagagc gttgatgagt aaagaacagg
     1561 taactatgga taaactggtc tctttggcta agagaagagg ttttgtatat cccggttcgg
     1621 agatttacga cggattggct aacgcatggg attacggtcc gttgggagtg gaactgaaga
     1681 ataatataaa agctgcctgg tggaagaaat tcattaccga taatgaatat aatgtcggac
     1741 tggactgtgc cattctcatg aaccccaatg tctgggtagc ttccggtcat gtcggatctt
     1801 tcgtggaccc gttaaccgat tgtaaaaact gtaaaacacg acacagagca gacgaactca
     1861 tcaaagaata caataaatca aaaagtatag agatgattgt tgacggattg gataacgcaa
     1921 cattaacgac atacataaaa gaaaagggaa taccttgtcc caattgcgga aaacaggatt
     1981 ttaccgaagt aaaacagttt aattgcatgt ttaagaccta tcagggtgtt aatgaagata
     2041 ctgccgctca aatctacctg cgaccggaaa cggcacaggg tatttttgtt aattttaaga
     2101 atgttcagag aacaacgaga agaaaaatac ctttcggaat aggacagata ggtaagagtt
     2161 tcagaaatga aataaccccg ggtaacttca ttttccgtac gagagaattc gagcagatgg
     2221 aactggagtt tttctgcaaa ccgggaaccg acctggaatg gtttgaatac tggagaactt
     2281 tctgccataa ctggctgtta agtttaggat tgaataagga taatattcgt ctgagagatc
     2341 attcggaaaa agaattgtct ttttacagta aagctactac cgactttgaa tatctgttcc
     2401 cgttcggttg gggggaactg tggggaattg ccgacagaac cgattatgac ttaaaacagc
     2461 attcggagtt ttccaaacag gatttatcat atttcgatcc gataacggaa gaaaaatata
     2521 ttccttactg cattgagccg tccgtaggcg tcgaaagact gctgttggca atactgaccg
     2581 atgcatatga tgaagaattg ctggaaaaag atgattccag aacggttttg cggcttcatc
     2641 ctttccttgc tcccgttaag gcggcgattc ttcctttagc aaagagactg ggtgatgatg
     2701 cgttcaaagt ctatgaaatg ataagaaaaa aatggcctgc ggaatatgat gatacccaga
     2761 gcatagggaa gcgatacaga agacaggatg aaataggtac acctttctgt ttcacatatg
     2821 attttgattc tttggaagat aattgtgtaa cggtaagata cagagacagc atgaagcaag
     2881 acaggataag aattgattca atcatgactt ttcttgagga aaatataaaa ctataatagg
     2941 aggtttttat gacgaaatat gtttatctct ttgaagaggg aaatgcgaaa atgagaaatc
     3001 tgctcggtgg caaaggagcc aaccttgcgg agatgacgac actgggatta ccggtgccga
     3061 gaggatttgt ggtaacaacc gaagcgtgcc tgagatatta tgaagacggc gagaagatcg
     3121 gtaaagatat agaagaagaa attcttaaaa tgctgaaagt taccgaaaag gccgtaggca
     3181 agaaattcgg tgatcccgag aatccgttcc tggtttcggt cagatcagga tcccgcgctt
     3241 ccatgccggg aatgatggac accgttttga acctgggact taacgacaaa gtagttgaag
     3301 gactggcaaa attaaccaat aatcccagat ttgcatatga cagctacaga agattcgtaa
     3361 tgatgttttc cgatgttgtt ataggtcttt caaaatccaa attcgaaaga atcttagatg
     3421 aaataaagaa agaaaaaggc attgttaacg acaccgatct tacagccgac gatatgaaat
     3481 ttgttctgaa gaagttt
//
LOCUS       DAOPWC010000131         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_27673_length_3470_cov_3.066471, whole genome shotgun sequence.
ACCESSION   DAOPWC010000131 DAOPWC010000000
VERSION     DAOPWC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27673_length_3470_cov_3.066471"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKJ65_03755"
     CDS             complement(<1..322)
                     /locus_tag="PKJ65_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNZ40984.1"
                     /translation="MKIISDFDMHLFREGTQCQAYRMFGAHLTESGCQFTVYAPNAKF
                     VSVVGDFNNWNLFAHNMDKSPEGVFSIFVPNAKEWDRYKYRIVTKNNKEIYKADPYAF
                     YAEHM"
     gene            complement(620..1489)
                     /locus_tag="PKJ65_03760"
     CDS             complement(620..1489)
                     /locus_tag="PKJ65_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ40985.1"
                     /translation="MKIGISSYSYYKLTRKGLIDCFGIIEKTKEFGFDAIEFSGMPGD
                     TYEERYKMAKDIKKACDDCNLLITNYCTGADLVKGSNWNLQTEIDRIKKEIDIAAVLG
                     VSKMRHDASFGFGATDPKARPLDMYLDRLAEGCKKLTEYAKGANIRTMIENHGRYCQH
                     SCDVLSIYRAVDDDNFGILIDIGNFMCVDQDPVEAVAQLAPYAFHVHAKDFHFKSGIN
                     MNPGWGWFLTKHGNYLRGSIIGHGDVPLKECINILKIYGYDDVLSIEFEGIEDVLSAI
                     ENGQANLKKLVDM"
     gene            complement(1499..2317)
                     /locus_tag="PKJ65_03765"
     CDS             complement(1499..2317)
                     /locus_tag="PKJ65_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ40986.1"
                     /translation="MSFKIGVITDSFRCPLEQAIDKAAALGADGVQIYATGRELAHDT
                     PDDRVKKILRLLKDAGLELSALCGDIGAFDDEIVNIERVKHSKKIVDLAVKMGTKVVT
                     THIGVVPEDKNHKQYRTIHKACSELGRYADSQGVKFAIETGPEKAYILRMLLDNLDCT
                     GIAVNFDPANLTMCVDDDAREAVKILAPFIVHTHAKDGIMLSKYANDPRYGELAKKRV
                     WLELPLGEGDVGFPTYLEALRKIGYKGFLTIEREVGEDPAKDIANAVTFLRKLI"
     gene            complement(2364..3419)
                     /locus_tag="PKJ65_03770"
     CDS             complement(2364..3419)
                     /locus_tag="PKJ65_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40987.1"
                     /translation="MLSNEIKKRYRAFWDRTAYERCVLNISQRIGKGVVMQGASYIDR
                     WTDLERRTRDAQESYVNFKYYAEGFSTVFTNFGPGSMSACIGGDFELAPSTIWFDRNP
                     VIKDWSEVPELKLYEDSLMWQFMTELTEMLCKASQRTYFTSMADIGGSMDILSSLRGA
                     EQLLCDLIDYPEEVKKVNLRIEELWKIVYSKLHAITSRYQNGMTSWMPIYCEKRYYPL
                     QCDFCAMISPDMFLEFVKPNLSAQAEWLDHSIYHLDGMDAVRHLDHLLDIPAIDAIQW
                     SAGAGKPPLYDECFFDMYNKISRSGKGLVLFSVPLTELENVLKNISTKGLFITMQVSD
                     EKEALEAIKIAKSYGVK"
BASE COUNT          942 a          847 c          595 g         1086 t
ORIGIN      
        1 gcatatgctc ggcatagaat gcatacggat cggctttata tatttctttg ttattctttg
       61 ttacaatcct gtatttatat ctgtcccatt cttttgcgtt tggaacaaat atactgaaca
      121 caccctccgg tgatttgtcc atattgtgtg cgaaaaggtt ccagttattg aaatctccca
      181 ctacggaaac gaatttggca ttgggagcat agacagtgaa ctgacacccg ctttcagtca
      241 ggtgtgctcc gaacatcctg tatgcctggc attgtgttcc ttcgcggaac agatgcatgt
      301 caaaatccga aataatcttc atgctaccct ctttcgttat tttatcatag aaacaagttt
      361 taataaatat gattacttcc gcaatgtcaa taatcggtta taactgcatc agatataggt
      421 aaaagaatgt tataggtaaa aaaccgttat ttcaaacacg gcagccgatt aaaaaatact
      481 ttaaaaatca agccaaaact accggcattc cgtatcagac gcttatcaat ttgtatttag
      541 ctgactgtgt aaacaagaaa aaagaacttg taacgacttg ggaataatat cctcttttgc
      601 tctgacagtc actgatatcc tacatatcaa ctaatttttt cagattggcc tgaccgttct
      661 ctatagccga gagcacatcc tcaatacctt caaattcaat tgacagcaca tcatcgtatc
      721 cgtatatctt aaggatattg atacattctt tgagcgggac atcgccgtgt cctataatgg
      781 agcctcttaa atagttgccg tgcttggtta aaaaccatcc ccagccggga ttcatgttaa
      841 tgccggactt gaaatgaaaa tcctttgcat gcacatggaa tgcataagga gccagttggg
      901 ctacggcttc aacggggtcc tgatccacac acataaaatt acctatgtct atcaatatac
      961 cgaaattatc gtcatccacc gccctgtata tgctcaaaac atcacaactg tgctgacagt
     1021 atctgccgtg gttttctatc attgtacgga tatttgctcc tttggcatac tcggttaact
     1081 ttttacaccc ctcagcaagc ctgtccagat acatatccaa aggacgtgcc ttggggtcgg
     1141 ttgctccgaa accgaaagat gcatcatgtc gcattttact taccccgagc accgctgcaa
     1201 tatctatctc tttttttata cggtctattt cggtctgcaa attccagttt gagcctttga
     1261 caaggtcggc tcccgtgcaa taattagtta tcaaaagatt gcaatcatca caggctttct
     1321 ttatatcctt tgccatctta tatctttctt catatgtatc gccgggcatt ccggaaaact
     1381 ctatggcatc aaatccgaac tccttggttt tctcaataat accgaagcaa tctatcaatc
     1441 cttttcttgt aagtttatag tatgaatatg agcttatccc tattttcatt attgcctcct
     1501 atataagttt acgtaagaaa gttaccgcat tggcaatatc tttcgcggga tcttccccga
     1561 cttcacgttc tattgtcaaa aaccctttat agcctatttt ccttaaagcc tcaagatagg
     1621 tcggaaaacc gacatctcct tcacctaaag gtaactccag ccatactctc ttctttgcca
     1681 gttcgccgta tcttgggtca ttcgcatatt tacttaacat aataccgtcc ttagcatggg
     1741 tatgtactat aaacggtgcc agtattttaa ccgcttctct tgcgtcatca tcaacacaca
     1801 tggtcagatt ggccgggtcg aagtttaccg ctattccggt gcaatccaga ttatccagaa
     1861 gcattcttaa tatgtatgct ttttccggac cggtctctat tgcgaatttc actccctgag
     1921 aatcggcata tcttcccagt tccgaacatg ctttatgaat tgtcctgtat tgcttatgat
     1981 tcttatcttc aggcaccact cctatgtggg tggttaccac cttggttccc atctttacgg
     2041 caagatccac tatttttttt gaatgcttaa ctctctcgat attgacaatt tcatcatcga
     2101 atgcgccgat atccccgcac agagcggaca attccagacc tgcatctttt aacagacgca
     2161 atatcttctt taccctgtca tcgggcgtat catgtgccaa ctctctgccg gtggcatata
     2221 tctgcacgcc gtccgcccct aaagccgcag ctttatctat cgcctgctcc aacggacatc
     2281 tgaacgaatc cgtaataact cctatcttaa aactcataat atgccctcca acatttattt
     2341 tacactatat tacactaata tatttactta actccgtagc tttttgctat tttaatagct
     2401 tctaaagctt ctttttcatc cgatacctgc atagtaataa acaaaccttt agttgatatg
     2461 ttcttcagaa cattttccaa ttcggtcagc ggcacggaaa aaagcaccag cccttttccg
     2521 ctccttgata tcttgttata catatcgaag aaacattcgt catataaagg gggcttaccg
     2581 gctcccgcac tccattgtat cgcatcaata gccggaatat ccagaaggtg atccagatga
     2641 cgaacggcat ccattccgtc aagatgatat atggaatggt ccagccattc tgcctgagcc
     2701 gataaattgg gttttacaaa ttctaaaaac atgtcgggag aaatcatggc gcaaaaatca
     2761 cactgcaaag gataatatct cttttcacag tatatcggca tccagcttgt cattccgttc
     2821 tgataacggg atgtaatagc atgcagtttg ctgtaaacaa tcttccacaa ctcctcgatt
     2881 ctcagattaa ccttcttaac ttcttcagga taatctatta agtcgcataa aagctgctct
     2941 gcacctcgca aagaggataa aatatccatc gaaccgccga tatccgccat ggatgtaaag
     3001 tatgtgcgtt gtgaagcttt acacagcatt tccgtcagtt cggtcatgaa ctgccacatc
     3061 aaactgtctt catacagttt caattccgga acttccgacc agtctttgat aacgggattg
     3121 cggtcaaacc atattgttga cggagccagc tcgaaatctc ctcctatgca tgccgacata
     3181 ctgcccggtc cgaaattggt gaacactgtg gaaaaacctt cggcataata tttaaagttg
     3241 acatacgatt cctgcgcatc ccgtgtgcgt ctttccaaat cggtccatct gtcgatatat
     3301 gaagcgcctt gcattacaac gcctttgcct atcctttggg atatattcaa tacgcatctt
     3361 tcatatgcgg tcctgtccca aaaggctcgg tatctcttct ttatctcatt acttaacata
     3421 aaacattcct ctcttagtca agtttgaaaa atctttctat cttcgcagtc
//
LOCUS       DAOPWC010000132         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_28057_length_3434_cov_3.563480, whole genome shotgun sequence.
ACCESSION   DAOPWC010000132 DAOPWC010000000
VERSION     DAOPWC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28057_length_3434_cov_3.563480"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKJ65_03775"
     CDS             <1..770
                     /locus_tag="PKJ65_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ40988.1"
                     /translation="HYCVTGERLNRSDIQFQPRRGIISPRTGDSEQEDNRYQTRQLLL
                     RMLREGNINYKQTLANSNHLFGFFIGAHRDSLIHATIGATGFTALCIHEAIAAGISSD
                     TAYAVGDAYNESMIQCRNIDELTAIAQEMYEDFVFRCHKHRMNPNLSPQILSCKEYIE
                     LYAEKPLHLATLAKRVGYSEYYLSRKFKKETGMSISNYIKYVRVERSKLMLAGAGVTI
                     AQIADTLHFASSSHFTESFKKITGMTPQQYRIENQQL"
     gene            858..931
                     /locus_tag="PKJ65_03780"
     tRNA            858..931
                     /locus_tag="PKJ65_03780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:890..892,aa:Gln,seq:ctg)
     gene            complement(965..1333)
                     /locus_tag="PKJ65_03785"
     CDS             complement(965..1333)
                     /locus_tag="PKJ65_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40989.1"
                     /translation="MYLFPYEEQYLSGKTENQIINTNSNLNLLLCSGECNRKYRPLAC
                     RIFPYFPYLDTNGNLEVKFDLRAKSICPLQFTDIPKIVINKRFIKRIERVFRGLIKHK
                     VFYDYLRYLTEEIVFLEKFH"
     gene            1614..1916
                     /locus_tag="PKJ65_03790"
     CDS             1614..1916
                     /locus_tag="PKJ65_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40990.1"
                     /translation="MAKKIYPDEYYKKKAVKLVVAHLRRKLEDAEHSDAETFDDWLLD
                     MDALLEKDDFVLAEYVEMRRKLNNLIDTIYDVKLRYKVRDSWNSFGRSLDKKAPIK"
     gene            1950..>3434
                     /locus_tag="PKJ65_03795"
     CDS             1950..>3434
                     /locus_tag="PKJ65_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40991.1"
                     /translation="MKNILKTLLILIITLILITTYVSVNASVAQDIYSSCEQKVIDNV
                     KYYKLTSVAGIHKLKVQWSDAEKSVTTSYNGRNIVFNTVNDIVYINGERHYFGDIVLH
                     NGFTYIPEDSITIFGEGYTAYQLNNSSNIKYVNGKITRIDIDANDKLTVTTLKKYDFN
                     QSLYFCVEEFAEMYSMTTKVAEGDYIMISNGKSDMYFHIQTDAIMYKGTFFTCGDIFI
                     KDGKHYAPDTITAFILEAFLPDFTRNENTSEQGEKEDKYTTYIFDNTDIKYVSLKELS
                     DIEGYKYQYYYVNKYAEVKAENISYFVLVPTNIIMKGDSIYNNFKVKEENDIVYIEYD
                     ALAVFGLIDDIKQPEPAGTINMGKDADKTQVAEPVFENKPDSETVTQYIPIRDLAQKV
                     GATVEWNDKTFCAILTVKDVKYVFDTRIMKIFVNEVEMPDCDFRFEGGITLVNTSFES
                     YINSSSVLHKALQSEDLLIIKSPNAKIKTHYPLNSPDRIVVDLLN"
BASE COUNT         1124 a          571 c          707 g         1032 t
ORIGIN      
        1 tgcactactg tgttaccgga gaacgtctga accgtagcga tattcagttt cagccgagaa
       61 gaggaataat cagtcccaga accggggatt ccgaacagga ggacaatcgt tatcaaaccc
      121 ggcagctgct actgagaatg cttcgggaag gtaatattaa ttataagcaa acactggcca
      181 attccaatca tctgttcggt ttctttatcg gagcccaccg ggattctctg attcatgcta
      241 ccatcggtgc caccggtttt acagcccttt gcattcatga agccattgcg gcaggaatct
      301 catcggatac tgcttatgcg gtaggagatg cctataatga gagtatgatt caatgtcgta
      361 atatagatga actgactgcc attgctcagg aaatgtacga agattttgta tttcgttgtc
      421 ataaacatcg aatgaatcct aatctctctc cccaaatcct aagttgtaag gaatatattg
      481 aactttatgc ggaaaaacct ctacacctag ccactttagc taagagagtg ggatattccg
      541 aatactacct gtcccggaaa ttcaagaaag agaccggtat gtccatcagt aactatatca
      601 aatatgttcg ggtagagcgg tccaaactga tgttggccgg tgccggtgtg accattgctc
      661 aaattgcgga taccttgcat tttgcttctt ccagtcattt tactgaaagc ttcaagaaaa
      721 ttaccggtat gactccgcag caataccgta tagaaaatca acaactgtaa caggtgcaaa
      781 gaggtttgtt ttttaggtgg aaggtcaaaa aagttgttga agtaataagg catttatggt
      841 atagtttctt ggtatattgc cgaattgtgt aatggtagca cacctgactc tggatcagtt
      901 tgtgagggtt caaatccttc ttcggcagcc aaaaggggat gtaaaggggg agtgcatcct
      961 ttttttaatg gaatttttcc agaaaaacaa tctcttccgt aagatatctt aagtagtcat
     1021 aaaagacttt gtgttttatt aatcctcgga aaactctttc tattcttttt ataaacctct
     1081 tattgatgac aatctttggt atatcggtaa attgcagagg acaaatactt tttgcacgta
     1141 aatcaaactt cacttccaag ttaccgttgg tgtcaaggta gggaaagtac gggaaaattc
     1201 tacaggctaa gggtcgatat tttctgttac attctccgct acaaagtaaa aggttaagat
     1261 tgctgtttgt gtttattatc tgattttcgg ttttgcccga aagatactgt tcttcatatg
     1321 gaaacagata catgccttcg tatccatctt tacaacactt gttattacat aattctccgc
     1381 agtcaaaatg taacggagta atgttattca ggagtttgta ggctctttta tatatacatt
     1441 ctatcttttt ttgtgtaata aggttattca taaggagatt atagcacatt gttgcatatt
     1501 gattatttca tttggttatg ttatcatgaa tttgtaaatt aatgagaatt ttaagtccgg
     1561 ttatgtatgt taatttttta ttagacagat ataacaaaag gatggtaagg gaaatggcta
     1621 agaaaatata tcctgatgaa tattacaaga agaaagctgt taaactggta gtggcacact
     1681 tgcgcagaaa actggaagat gcggaacaca gtgatgctga aacttttgat gattggcttt
     1741 tggatatgga tgcactgctg gaaaaggatg attttgtttt agcggagtat gttgagatga
     1801 ggagaaaact taataatctc attgacacca tatacgatgt caagctcaga tataaagttc
     1861 gtgattcatg gaacagtttc gggaggagtt tggataaaaa agcaccgatt aaatagataa
     1921 acttaaatag gggtacaaaa gataaaacaa tgaaaaacat actaaagaca ctcttgattc
     1981 taattattac tttaattcta atcacaacat atgtaagtgt gaatgcatct gtggcacaag
     2041 acatatattc ttcatgtgag caaaaagtta ttgacaatgt aaaatactat aaactgacat
     2101 cggttgcggg tatacacaaa cttaaagtgc aatggagtga tgccgagaaa agcgttacaa
     2161 cgtcatataa cggcagaaat atcgttttta atacggttaa cgatattgtg tatattaacg
     2221 gtgaaaggca ctatttcggt gatatagtgc tgcacaacgg cttcacatac ataccggaag
     2281 actcaataac gatattcggt gaaggatata cggcttatca attaaataac agcagtaata
     2341 taaagtatgt taacggaaag attaccagaa ttgatattga tgcaaatgac aaacttacgg
     2401 ttacaacttt aaagaagtat gattttaatc agtcactcta tttctgcgta gaggaattcg
     2461 cggaaatgta cagtatgaca acaaaagtag cggaaggcga ctacatcatg ataagcaacg
     2521 gaaaaagcga tatgtatttt catattcaga ccgatgctat catgtataaa ggtacatttt
     2581 tcacttgcgg tgatatattc attaaagacg gtaagcatta tgctccggat acaataaccg
     2641 cctttattct ggaagctttc ctgccggatt ttacaaggaa cgaaaataca tccgaacaag
     2701 gtgaaaaaga ggataagtac actacataca tattcgataa cactgatatt aaatatgtga
     2761 gtctgaaaga attaagcgat atcgaaggat acaaatacca gtactactat gtaaataaat
     2821 atgccgaagt gaaggcggag aatatatcat acttcgttct ggttcccacc aatattatta
     2881 tgaaggggga tagtatatac aataatttta aagttaagga agaaaatgac attgtatata
     2941 ttgaatatga tgctctggcg gtcttcggac tgattgacga cattaagcaa cccgagcccg
     3001 cgggtacaat caatatgggt aaagatgcgg ataaaactca ggttgccgaa cctgtttttg
     3061 aaaataagcc tgattcagaa acggttactc aatatattcc tatcagagac cttgcacaaa
     3121 aggtaggagc aacagttgaa tggaatgaca agacattctg tgcaatcctg acagtcaaag
     3181 atgtgaaata tgtgtttgat accaggataa tgaagatttt tgtcaatgaa gtggaaatgc
     3241 ctgactgtga tttcaggttt gagggcggta taacgcttgt aaatacaagc tttgagtcct
     3301 atattaattc ctcgtcggtt ttacataagg ccttacaatc ggaagatctc cttataatca
     3361 aatcgccgaa cgcaaaaatt aaaactcatt atccgttgaa ttctcccgat cgtattgttg
     3421 tggatttatt gaat
//
LOCUS       DAOPWC010000133         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_28357_length_3404_cov_3.086892, whole genome shotgun sequence.
ACCESSION   DAOPWC010000133 DAOPWC010000000
VERSION     DAOPWC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28357_length_3404_cov_3.086892"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..594)
                     /locus_tag="PKJ65_03800"
     CDS             complement(151..594)
                     /locus_tag="PKJ65_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ40992.1"
                     /translation="MTKQPELNEALLNAIHRFTVTLRRKRISADIIFHEFLLLRKIQR
                     IQSEKNDPVYPSDLSDKLNLSKSYITAVLNSLEKKELIVRTLDTADRRRLPVRITDKG
                     RQIFDNMAAKELEQTDILVSGLTREKTLNLISLLNQASDILDKGE"
     gene            741..1802
                     /locus_tag="PKJ65_03805"
     CDS             741..1802
                     /locus_tag="PKJ65_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ40993.1"
                     /translation="MELTGIERISRILKRQPVDRIGLYEHFWSDTHKAWGILTDIEER
                     MGFDMIEAWPFVMIADLDFERITVAEDEDTVTQKDGNYAMLRKHKKHDTTPEHVGFEI
                     DSREKWEERIKPLLTPSERRINFERYRIKKQYAKDRDMYFVWSGANVFELMHPLTGHV
                     NFLMAMIDDPEWVEDMCMTYAKLTVELQQILFEKEGYPDGIWYYEDMGFKNAPFISNA
                     MYDRFIYPAHKLTIDYAKSCNLPVIMHSCGYVEPLIPGMLKAGIDCLQVIEIKAGMDL
                     LRIHKNYKDCLSLMGGIDVRTLYSNDKEIIDRELESKIPVVKEGYGYVLHSDHSIPNT
                     VNLETYEYFIKKGLELGKY"
     gene            1818..>3404
                     /locus_tag="PKJ65_03810"
     CDS             1818..>3404
                     /locus_tag="PKJ65_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HNZ40994.1"
                     /translation="MRSKSDRKKKVHLMCNAHIDPVWLWNWEEGAAATISTFRSAVRF
                     CKEYDEFVFNHNESVLYEWIEEYDPALFEEIKELIKAGKWHVIGGWYLQPDTNMPSGE
                     SFVRQIEEGRRYFLEKFGVDGGKTAINFDPFGHTIGLVQILKDAGYENYIVSRPAKLI
                     YDGSSLHDKHILWKGFNNKSINVFKATTYGSRLGEFLATKYDYLYDNQKDKNDIGMLW
                     GVGNHGGGPSKKDLDDIRELTSKSKDEIVHTWPDKFFEEYFRRNQSDRVIKRSLHSFS
                     VGCYTSMVRIKQKHRELENKLLLCEKMMSHAVANDSLEKYEHQMLRDAWKNLLFCEFH
                     DSLPGSSIQIVENDILKRLDHGLDIADRLIFRAYFRLVKGQKPAKEGEYPVLVYNPHP
                     YRVKTVVECEYCLAEQNRVPDTFSKGDVFDEKGNLVACQNEKESSNIPIDWIKRVAFL
                     ADLAPMSVNRFNIVNRLVEKKPFELPKTDDNYIYFDNGTMQSKVNRKTGHIDSYKVNG
                     TEYIKPGMCQMLVIQDNSDSW"
BASE COUNT         1093 a          588 c          785 g          938 t
ORIGIN      
        1 tgtatctcct gtcggtatcc cgatgttgac tatttccgac atataagcgg gcagatacag
       61 gtttgccatt gactgggcga atgtcagtgc caatatcaaa gcaatcttcc accagtcatt
      121 tttcaagaat ctgagtattt ttcccactgt ttactctcct ttatccaaaa tatcactggc
      181 ttgattcaga agtgatataa gattcaatgt tttctctcgg gttaagcccg atacgagaat
      241 atccgtctgt tccaattcct ttgcggccat attatcaaat atctgacgtc ctttatcggt
      301 aatgcgcacg ggaagcctgc gacggtcggc tgtatccaat gttctgacaa taagctcttt
      361 tttctccaga gaattgagga ccgcggttat ataggatttt gataaattca gtttatcgct
      421 taaatcggaa gggtatacgg ggtcattttt ctctgattgt attctttgaa ttttccttag
      481 aagcaaaaac tcgtgaaata tgatatcggc agatattctt ttacgacgca atgtgacggt
      541 aaatctgtga attgcattca gaagcgcttc atttaactcc ggttgtttag tcattttact
      601 gtctccaaat taattaattt agcaaatgaa tagttcatat attgaattat atttatgcct
      661 tttttaaagt caacataatt attcgatttg tctgctattt tatgtgcatt ctgttataat
      721 tatcctgacg aggtttattt atggaattaa cggggataga aagaatatca aggatactga
      781 aaaggcagcc tgtagacagg ataggcctgt atgagcactt ctggtcggat acacataaag
      841 catgggggat attaaccgat atagaagaaa gaatgggctt tgacatgata gaagcatggc
      901 cttttgtaat gattgcggat ctggattttg aaaggattac cgttgcggaa gatgaagata
      961 cagttactca gaaagacggt aattacgcaa tgctgagaaa gcataagaaa catgatacta
     1021 cccccgagca tgtaggcttt gaaattgatt caagggaaaa atgggaagaa aggataaaac
     1081 cgctgcttac cccttcggaa agacgtatca attttgaaag atacagaatc aagaagcagt
     1141 atgccaaaga tcgggacatg tactttgtat ggtcgggagc aaatgttttt gagttgatgc
     1201 atccgttaac cggacatgtc aattttctta tggccatgat tgacgatccc gaatgggtgg
     1261 aagatatgtg catgacatat gcaaagctta cggtggaact gcagcagata ttgtttgaaa
     1321 aagaaggata tcccgacggt atctggtatt atgaagatat gggattcaag aatgctcctt
     1381 ttatatccaa tgccatgtac gatcgattca tatatccggc acacaagctt acaatcgact
     1441 atgcgaagag ctgtaacctg ccggttataa tgcattcctg cggatatgtg gaacctctta
     1501 taccgggaat gcttaaggcg ggaatagact gcttacaggt tatagagata aaagcgggca
     1561 tggatttact gaggattcat aagaactata aagactgtct ttcccttatg ggaggtattg
     1621 atgtcagaac actatattca aatgataaag agataattga cagagaactg gaaagtaaaa
     1681 tacctgttgt taaggaagga tacggatatg tactgcattc cgatcattcc atacccaata
     1741 cggtcaattt ggagacatat gagtatttca ttaaaaaagg attggaatta ggaaagtatt
     1801 aacgggagga gtaattcatg aggagcaaaa gcgacaggaa aaagaaagta catcttatgt
     1861 gcaatgcaca tatagatccg gtatggttat ggaattggga ggaaggagcg gcagccacaa
     1921 tatccacatt ccgttcagcg gtaaggtttt gtaaggagta cgatgaattt gtattcaatc
     1981 ataatgaatc ggtactttac gaatggatag aggagtatga tcccgcttta tttgaggaaa
     2041 taaaggagtt gataaaggcc ggaaaatggc acgtaatagg cggatggtat cttcagcccg
     2101 atacaaatat gccttcagga gaatcttttg ttcgtcagat tgaggaaggc agaagatatt
     2161 ttttggaaaa attcggtgtt gacggaggaa aaaccgcaat taactttgat cctttcgggc
     2221 atacaatagg tctggtgcag attttaaagg atgcgggata tgaaaactat atagtatcca
     2281 gacctgcaaa acttatatac gacggcagca gtctgcatga taagcatatt ctatggaaag
     2341 gatttaataa taaatccata aatgtattca aggctacaac atacggctcc agattgggag
     2401 aattccttgc aaccaaatat gattatctgt atgacaatca gaaggataaa aacgatatag
     2461 ggatgttatg gggagtgggt aaccacggag gcggaccttc aaagaaagat ctggatgata
     2521 taagggaatt gacctccaaa tcgaaagacg agatagtgca tacctggccc gataaattct
     2581 ttgaggaata tttccggaga aatcaaagcg acagagtaat caaaagatcg ctgcattcct
     2641 tcagtgtcgg ttgttacacg tcaatggtaa gaatcaaaca aaagcacaga gaattggaaa
     2701 acaagttgct tttatgcgaa aagatgatga gtcatgcggt agctaacgac tcgttggaaa
     2761 aatatgaaca tcagatgctg agggatgcat ggaagaatct tctgttctgc gaatttcatg
     2821 attcgctgcc cggtagcagc atacagattg tggaaaacga tattttaaag aggctggatc
     2881 acggattgga tatagcggac agattgatat tcagagcata tttccgcctt gttaaagggc
     2941 agaaacccgc aaaagaaggg gaatatcccg ttcttgtata caatccgcac ccttacagag
     3001 tcaaaaccgt tgtagagtgc gaatactgtc ttgcggaaca gaacagagta cccgacacct
     3061 tctctaaagg cgatgtgttt gacgaaaaag gcaatcttgt ggcgtgccag aatgaaaagg
     3121 aaagttccaa tattcccatt gactggataa agcgagtggc tttcctcgcg gatctggcac
     3181 ccatgtcggt caacagattc aatatagtaa acagactggt ggagaagaag ccttttgaat
     3241 tgccgaaaac ggacgataac tacatttact ttgataacgg aacaatgcag tcaaaagtaa
     3301 accgaaagac cggtcatata gattcctaca aagtcaacgg cacggaatac atcaaacccg
     3361 gtatgtgtca gatgctggtt atacaggaca actccgattc ctgg
//
LOCUS       DAOPWC010000134         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_28559_length_3386_cov_3.888022, whole genome shotgun sequence.
ACCESSION   DAOPWC010000134 DAOPWC010000000
VERSION     DAOPWC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28559_length_3386_cov_3.888022"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKJ65_03815"
     CDS             <1..188
                     /locus_tag="PKJ65_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ40995.1"
                     /translation="KRAALETFADAKSISGWATDAMAWANAEALILGDGTGLNPSGKG
                     ERCHAAAIFQRFIANIR"
     gene            complement(291..1004)
                     /locus_tag="PKJ65_03820"
     CDS             complement(291..1004)
                     /locus_tag="PKJ65_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ40996.1"
                     /translation="MKGFLYGLALQCKLDLRSKTILVTCYIVPLLFFLLMGSIFGSVM
                     PEMKETLIPSMIVMSVSMGAFIGLPPSLIETYGTDVKKVYKANGIPLHVGLITMFLST
                     FFHLMISCVIILLLAPVLFDAASPKNIPLFLLALAIYAIVSLSIGSILGLVVKSQAKV
                     TMIAQLLFLPSIMLSGIMLPIEILPESLKILGRIFPATWGYRLMLDNGFHIKNLWYLI
                     IVFCIAVIVCMILLKKTEI"
     gene            complement(1001..1816)
                     /locus_tag="PKJ65_03825"
     CDS             complement(1001..1816)
                     /locus_tag="PKJ65_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ40997.1"
                     /translation="MNCAIQVSNLRKSYGSNAVLKGINLHIKEGEIFALLGVNGAGKT
                     TALECIEGLKKYESGEISVNGEMGIQLQSSSLPAQIKSMEAVKLFSKWKKANIDYEML
                     TALGIEEIKKKKYIELSTGQKRRLHLALALIGNPDIIFLDEPTAGLDVEGRLSLHEQV
                     RKLKSQGKTIVLASHDMAEVEALCDRIAILRNGNIVFCGTAAELTEKLGRKYLVHIKT
                     QQGAQTFATDNIEDTLLSLLGELKQKGLYISDIKVDRGTLEQHFIEMTRGEEE"
     gene            complement(1813..2613)
                     /locus_tag="PKJ65_03830"
     CDS             complement(1813..2613)
                     /locus_tag="PKJ65_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ40998.1"
                     /translation="MAKYKAIPDGYMTIGKAAKKMGVTVRTLQYYDKEGLLSPSGESE
                     GGRRLYTDKDLVVLHQIVSLKSLGFSLNDIKQRLISLDTPADVANALTEQADKIRSEI
                     ENLTVSLNAIEQLKTEVLQMQKVDFKKYADIIVNLQMNNKYYFLIKKFDDDTLDHIRD
                     RFDKESSMDFINRFDALSDEILKLQKENVPQESEQCQQLTKAYWDLIMEFTNGDMSML
                     QKLPEIANFENTNTDWAKKQAIVNSYLEPALVIYFKKLGINNVGEEQQ"
     gene            2800..>3386
                     /locus_tag="PKJ65_03835"
     CDS             2800..>3386
                     /locus_tag="PKJ65_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ40999.1"
                     /translation="MMSRLFYVSDFLKEGISDSQAIKDCLNSAKSCDERTIIFDKKDY
                     YIDAAITLPSDTTVIIDGVMIKQNDFVHDNVFRADNMETDPDNPYGYPLSIKAAKDIK
                     IIGKNDALIEGPEKHTSFWHPHFNEEQLAIGDYWGWRGFQMLISRCENFEIRDITFKK
                     VKSWTISADRSKNLHFSDLRFYSTVKNGDGLNIRAG"
BASE COUNT          960 a          709 c          658 g         1059 t
ORIGIN      
        1 ccaagagagc cgcactggaa acatttgcag atgcaaagag tatatccggc tgggcaacgg
       61 atgctatggc atgggcaaat gccgaagctc tcatattggg tgacggcaca ggcttgaatc
      121 cttcaggaaa aggagagcgc tgtcatgcag cggcaatatt ccagaggttt attgcaaaca
      181 taagataaaa tcttttataa tatcctcatg acaaaggtgc cacgcttccg ccaaaggtgt
      241 ggcacctttg ctgtcgttat ctcataaaat tccactaagc acacagtcat ttaaatttct
      301 gtttttttca gaagaatcat acatactata accgcaatac aaaaaacaat tatcagatac
      361 cacaggtttt taatatgaaa gccgttatcc agcatcagtc ggtatcccca tgtggcggga
      421 aaaatacgcc ctaatatttt cagtgattcg ggcaatattt caataggaag cataattccc
      481 gaaagcataa tggaaggcaa aaacagcagt tgtgcaatca tggttacctt tgcctgactc
      541 ttgacaacca gtcccaatat gcttcctatg ctaagagaaa caatagcata tattgcaagt
      601 gcaagaagaa acagaggaat gtttttcggt gatgccgcat caaacagtac cggggcaagc
      661 agtagtatta tcacgcagga aatcatcaga tgaaagaatg tggataaaaa catggttata
      721 aggccaacat gcagagggat tccgtttgct ttatacactt ttttaacatc ggttccgtaa
      781 gtttcaatca aggaaggcgg caatcctata aatgctccca ttgacacact catcacaatc
      841 attgacggaa tgagtgtttc tttcatttcc ggcataaccg aaccgaaaat actgcccatt
      901 aaaaggaaga aaagaagagg gacaatatag caggttacca atatagtttt actccgtaaa
      961 tccaatttgc attgtaatgc aagaccgtat aaaaaaccct tcattcttct tcaccccttg
     1021 tcatttcaat aaaatgttgt tccagtgtcc ctctgtccac ttttatgtcc gatatatata
     1081 atcctttttg tttcagttca ccgagcaggg acaataatgt atcttctatg ttgtccgtag
     1141 caaaagtctg agccccttgt tgtgtcttta tatgtaccag atattttctt cccagttttt
     1201 cggtcagttc cgcagcggtg ccgcaaaaga caatattacc gttacgcaga atggcgatgc
     1261 gatcacacaa cgcctccact tccgccatat catggcttgc aagaacaatg gtttttccct
     1321 gtgatttaag ttttcgtacc tgttcatgca gtgacagtct accttcaaca tccagtcccg
     1381 cagtgggctc gtctaagaat atgatgtcgg gattacctat aagtgcaaga gcgagatgca
     1441 gccgcctttt ttgtccggtc gataactcta tatacttctt tttcttaatc tcttctatgc
     1501 cgagggcggt aagcatttca tagtctatat ttgctttctt ccatttggaa aacagtttga
     1561 ctgcttccat ggattttatc tgtgcaggca aagaggagga ttgtaactga atacccattt
     1621 ctccgtttac ggaaatttca ccgctttcat actttttcaa tccttctata cattcaaggg
     1681 ctgtggtctt tcccgcgccg tttactccta aaagagcaaa aatctctccc tcttttatat
     1741 gcagattgat acctttgaga acggcattgc tgccgtagct ttttcttaaa ttacttacct
     1801 gtattgcaca gttcattgtt gctcctctcc tacattatta attccgagct ttttgaaata
     1861 aatgaccaaa gcaggctcta aataactgtt gacaatcgct tgctttttag cccagtcggt
     1921 atttgtgttt tcaaaattgg ctatttccgg taacttctgt aacatactca tgtcaccgtt
     1981 tgtaaactcc ataatcaaat cccagtaagc ctttgtcagt tgctgacatt gctcgctttc
     2041 ctgcggtaca ttttcttttt gaagtttcag tatttcgtca cttaaggcat caaatctgtt
     2101 aataaaatcc atactgctct ctttgtcaaa acggtcacgg atatgatcca gtgtatcatc
     2161 atcaaatttt ttaataagaa agtaatattt attgttcatc tgaaggttaa cgattatatc
     2221 ggcatatttc ttaaaatcca ctttttgcat ttgcaatact tcggttttta actgttctat
     2281 ggcattaaga gaaacagtca ggttttctat ttcactgcgt attttgtctg cctgttcggt
     2341 aagggcattt gccacatcag ccggtgtatc cagagatatc aaccgctgct ttatatcatt
     2401 taaagaaaaa cccaatgatt tcagcgatac aatctgatga agcacaacca ggtctttgtc
     2461 ggtatagagc ctgcgaccgc cttcactttc gcccgaaggc gacagcagtc cttctttatc
     2521 gtaatactgt agtgtgcgga ccgttacacc cattttcttg gccgctttac ctatggtcat
     2581 atatccgtcc ggaattgctt tgtattttgc cataatcatt tgcctccgcg tatattataa
     2641 ttcatgacgc tacgtcacga acaatacctt tttaattaat tttcatagtt ttttcttttt
     2701 cttgcattta ccggttattt tgccgtttgt ttgacaccga taaccggttg tattacaatc
     2761 ggttagaccg gtattcggta taaagataag ggcggcggaa tgatgagcag gttgttttat
     2821 gtttcggatt ttttgaaaga gggaataagc gacagtcagg caataaaaga ttgcctgaac
     2881 tctgctaaaa gctgtgatga aagaacaata atatttgata aaaaagacta ctatattgat
     2941 gcagcaatta cattgccttc cgacaccacc gtcataattg acggggtgat gattaagcag
     3001 aatgactttg tgcatgacaa tgtattccgt gcggacaata tggagacaga tcccgataat
     3061 ccttacggtt atcctttatc tataaaagcg gctaaggata ttaagattat agggaagaat
     3121 gatgcgctga tagaagggcc ggagaagcac acaagttttt ggcatcccca ttttaatgaa
     3181 gaacaacttg ccatagggga ttactggggc tggcgcggat tccagatgct tatttcccgt
     3241 tgtgaaaatt ttgaaattcg tgatattact tttaaaaaag tgaagagttg gacgatttcc
     3301 gcagaccgct ctaaaaatct gcatttttcc gatctgcggt tttattccac ggtaaaaaac
     3361 ggagacggtt tgaatatacg ggcagg
//
LOCUS       DAOPWC010000135         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_28623_length_3381_cov_3.782020, whole genome shotgun sequence.
ACCESSION   DAOPWC010000135 DAOPWC010000000
VERSION     DAOPWC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28623_length_3381_cov_3.782020"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="cysS"
                     /locus_tag="PKJ65_03840"
     CDS             <1..748
                     /gene="cysS"
                     /locus_tag="PKJ65_03840"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ41000.1"
                     /translation="GWHIECSAMSRKYLGDQFDIHTGGVDHIPIHHENEIAQSDAFLK
                     KQAVKYWMHCEFLQVDGGKMSKSMGTAYLLSDLEEKGYEPLAFRYMCLNAHYGKKLNF
                     TWDGIEAAQKSLNRLSNLVLEHKESDTHIDSNVIEEYKQQFREALQDDLNLPLAMSTV
                     WNAARNNVKSKSIYNLLMDFDKVLALDLDKKRTEAAEDIPEEVTLLAKQRLEARKNKD
                     WKKSDQLRDRIRQLGYSINDTPAGQVIKKG"
     gene            750..1610
                     /locus_tag="PKJ65_03845"
     CDS             750..1610
                     /locus_tag="PKJ65_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNZ41001.1"
                     /translation="MLIKVFQKGFNFSQDGPGNRLVYHLQGCNMNCPWCSNPEGIEKD
                     GSIMVTEGVDDAVCPNGAISSGMLDSKLCRGCPKMCLQTLHMGIKLSCDTYHTDEIVS
                     EAVRSKSMFFSGGGVTFTGGEPTLNLPALTDMLKKLKQEDINTAIETNGSHPDLASAT
                     EYIDHLIIDFKHYDDDRHRKTVGISNAIIKKNLKRLSLIRKINIRIPLIKGFNAEEEN
                     AVGFVNTLSECNKQNITVEFLSFHEFGKSKWQQCGMEYKMKGDEYISDDTLEMFTRIF
                     KDNGFDIIRT"
     gene            1626..>3381
                     /locus_tag="PKJ65_03850"
     CDS             1626..>3381
                     /locus_tag="PKJ65_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNZ41002.1"
                     /translation="MNVYEVYTQKAIDDKASLLFKEERSLKRLDGWFLAREIVCDTHE
                     ELKDLPKELRAAYEIKNIITNIPLGISENNIFAGTQRDAFARSYALINPSFRVETFSG
                     YCDPTAIYDDIEPNEEFTRERIAKVKEFTKNTEFVKKLTKVYDDYEQYTKEVIFFVEQ
                     VTGHVIPDFRPALKYGVKAIIENTEQRIKNEENEQKRNNLAAMKIALECVVQLANRYA
                     DIAASQYKEACAERKKQLALLESTLRKVPALPSENLYEAIQAYLLLWQVMCLEQAPNP
                     FAFSVGNADRIFEPYRKNLTREQAASLFRHFLVFYNVGDRSWAISQNVLLGGKSNSGV
                     DLTNVCTYALLDAYYAMNFPQPILSVKLHKNTPQKLYEEMGRFFFTPGCLTPSFFNDD
                     SVFQVLKKAGIEEADLEDYSVAGCQEPLIMGKDNGNTTNSWLNLGKILEMTINNGRSL
                     ISGEQLGRKRNLDNLNILGNIKEYFYDDAKYYIKHMADAANGASVALSELPVPFLSSF
                     MGGLETGYDMRDVKNQGTRYNASGCLIHGLSVVADSIIAIEEYLKVYPDNPDRLINAL
                     RNNFEGDSDIKAFLKNAPK"
BASE COUNT         1119 a          679 c          756 g          827 t
ORIGIN      
        1 cggatggcat atagaatgtt ccgcaatgag cagaaagtac ttgggtgatc agtttgatat
       61 acataccgga ggagtggatc atatacccat acaccatgaa aatgaaatag cgcagtcgga
      121 cgcttttttg aaaaagcagg cggttaagta ctggatgcac tgcgagtttt tacaagttga
      181 cggaggtaag atgtccaaat ccatgggaac agcctatctt ttgtccgact tggaagaaaa
      241 agggtacgaa cctcttgcct tccgatatat gtgcttaaac gctcattacg gaaaaaaact
      301 gaacttcaca tgggacggaa tagaggcggc tcagaaaagt ctcaaccgtt tatccaatct
      361 cgttttagag cacaaagaat cggatacaca cattgactca aacgtgatag aagaatacaa
      421 acagcaattc cgtgaagcat tgcaagatga cctcaattta cctcttgcca tgagtaccgt
      481 atggaatgcg gccagaaaca atgtcaaatc aaaaagcata tataatctgc tgatggattt
      541 cgataaggtg cttgctctgg atcttgacaa aaaaagaacc gaagcggcgg aagatatacc
      601 ggaagaagta acactacttg ccaagcagcg tctggaagcc agaaaaaaca aagactggaa
      661 gaaatccgac cagttgcgtg accggataag gcagttgggt tattccataa acgatacacc
      721 ggcaggtcag gtgataaaga agggataaaa tgcttattaa agtatttcag aaaggcttta
      781 atttctcaca ggacggaccg ggtaacagac ttgtttatca tctgcaaggt tgcaacatga
      841 attgcccgtg gtgttccaat cccgaaggta tagaaaaaga cggcagtata atggtaaccg
      901 aaggtgttga cgatgccgta tgtcctaacg gtgcaatatc ttccggtatg ctggacagca
      961 aactttgcag gggctgtcct aaaatgtgtc ttcagacact ccacatgggt attaaattat
     1021 cctgtgatac ataccacacc gatgaaatag tttcagaagc cgtaagaagc aaatccatgt
     1081 tcttttccgg aggcggcgtt acctttaccg gaggagaacc gacattaaat ctgcctgctt
     1141 tgaccgacat gctgaaaaag ttgaaacagg aagatattaa taccgctata gagaccaacg
     1201 gttcccatcc cgatcttgca tcggcaacgg aatatatcga ccatctcatt atcgacttta
     1261 aacactatga tgatgacagg cacagaaaaa ccgtgggtat ctccaatgca ataattaaga
     1321 aaaacctgaa aagattatcg cttataagaa aaatcaacat acgaataccg ctcataaagg
     1381 gttttaatgc ggaagaggaa aatgccgtgg ggtttgtaaa taccttatcg gaatgtaaca
     1441 agcagaacat aaccgttgaa tttttgtcat ttcatgaatt cggcaaatcc aaatggcaac
     1501 aatgcggtat ggagtataaa atgaaaggcg atgaatatat ttccgatgac actttggaaa
     1561 tgtttacccg tatatttaaa gataacggat ttgatataat aaggacatag ctttcggagg
     1621 tagacatgaa cgtatatgaa gtttacacac aaaaagcaat agatgacaag gcctccctgc
     1681 ttttcaaaga agagcgcagt ttaaaaagac tggacggttg gtttcttgct cgtgagattg
     1741 tttgcgatac gcatgaagaa ttgaaagatt tacccaagga acttcgggca gcttatgaaa
     1801 ttaaaaacat tatcacaaac ataccgctgg gcataagcga aaacaatatc tttgcaggaa
     1861 cgcaaagaga tgcatttgcc aggtcctatg ccctcataaa tccctcattc cgggtggaaa
     1921 cattctccgg ctactgcgac cccacagcga tatatgatga tattgaacct aacgaagaat
     1981 ttacaagaga acgcattgcc aaagttaagg aatttaccaa gaatacggaa tttgtaaaga
     2041 aacttacaaa agtctatgat gactacgaac aatacacaaa ggaagtaata ttctttgtgg
     2101 aacaggttac gggacatgtg ataccggatt tccgtccggc tttaaaatac ggtgtaaaag
     2161 ccataataga aaatacggaa caacgcataa aaaacgaaga aaacgagcag aaacgaaaca
     2221 accttgctgc aatgaagatt gctcttgagt gtgttgtgca gcttgcaaac agatatgccg
     2281 atattgcggc atcgcaatac aaagaagctt gtgccgaaag aaaaaagcag cttgcgcttt
     2341 tggaatccac actgagaaaa gttcctgccc tgccgtcgga aaatctatat gaggcaatac
     2401 aggcatacct gttattatgg caggtaatgt gccttgagca ggctcccaat ccgtttgcct
     2461 tttccgtagg aaatgcggac aggatattcg agccttacag gaaaaatctt accagagagc
     2521 aggcggcaag tttattcagg catttccttg tattctataa cgtgggagac cgatcctggg
     2581 ccatatcgca gaatgtactc ttaggaggca aatccaattc cggtgtcgac cttaccaatg
     2641 tctgcacata tgcattactg gacgcatact atgccatgaa cttcccgcaa cccatattat
     2701 cggtcaaact gcataagaac acccctcaaa aactgtatga agagatggga agatttttct
     2761 ttacgcccgg gtgtcttact ccttctttct tcaatgacga ctcggttttc caagtgctaa
     2821 aaaaagcggg tattgaagaa gccgacctgg aggattattc ggtagcaggc tgtcaggagc
     2881 cgttaattat gggtaaagac aacggcaata ccaccaacag ctggctgaac ctcggtaaga
     2941 tactggaaat gacaataaac aacggacgct cccttatatc cggcgagcag ctgggaagaa
     3001 aaagaaatct tgataatctt aacatactgg ggaatataaa agagtacttt tacgacgatg
     3061 cgaagtatta tataaaacat atggcggatg cggcaaacgg cgcttcggtt gctctgtcgg
     3121 aactgccggt acctttctta tcaagcttta tgggcggatt ggaaaccggc tatgacatga
     3181 gagatgttaa aaaccaggga accaggtaca atgcaagcgg atgccttatt cacggtttat
     3241 cggttgtcgc cgattccata atagcgatag aagaatactt aaaagtatat cccgataatc
     3301 ccgaccgcct tataaacgct cttaggaata attttgaagg cgattccgat ataaaggctt
     3361 ttctgaaaaa tgctcccaag t
//
LOCUS       DAOPWC010000136         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_28899_length_3358_cov_3.066606, whole genome shotgun sequence.
ACCESSION   DAOPWC010000136 DAOPWC010000000
VERSION     DAOPWC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28899_length_3358_cov_3.066606"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /gene="uvrC"
                     /locus_tag="PKJ65_03855"
     CDS             complement(<1..1405)
                     /gene="uvrC"
                     /locus_tag="PKJ65_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ41003.1"
                     /translation="MTVLNEKIAYLLSTCPEKPGVYLMKDNKGNTIYVGKAVNLKNRV
                     SSYFTNMSAHSYKTKKMVSNIETFEYIVLDNERQAFLTESDLIKSLRPKYNILMKDDK
                     SYPYIKITTQEQFPGIYVTRNRSDKNAKYFGPYPGRVSKKNTVTLLQSAFMLRSCKKM
                     AKRPCINYDMHLCLAPCSEDCDKNKYREAVDAALRFLSGNGRDLIDEYKSKMQAESDN
                     LNFEKAAYYRDRINLLTTISDNYILDTDRNSADIIAYARMKDFASFFIMNIRDGKVAD
                     KKTVNIHQERIEDTDIGYEFIRQYYDTNIPKEIVVYNEIMNREDTEQWLFNNCGRKVS
                     ITVPKKGKLKKLADAAYENAVVTLNLFMGQHKNSAGTSMVYSLASFLGLETVPDVIEA
                     FDISHLSGQDAVASQAVFKEGVPYKSGYRRYIIKGDNTRSDTDSLREVMERRMKRGDY
                     PDLFLIDGGFNQVAAVCD"
     gene            complement(1406..1849)
                     /gene="rimI"
                     /locus_tag="PKJ65_03860"
     CDS             complement(1406..1849)
                     /gene="rimI"
                     /locus_tag="PKJ65_03860"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNZ41004.1"
                     /translation="MNIRIKRADINDVDRIFAIEKETFNDYWSYESLYEDICRTPMSF
                     YLAAVFDDEVVGYIGMWHVLDEAHIMNIAVDKRYRNKGIGTLLLSALIDYSREVEIKR
                     MTLEVREHNDAALRLYEKHGFVRDGIRKEYYRDTNEDAVIMWKEL"
     gene            complement(1846..2550)
                     /gene="tsaB"
                     /locus_tag="PKJ65_03865"
     CDS             complement(1846..2550)
                     /gene="tsaB"
                     /locus_tag="PKJ65_03865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ41005.1"
                     /translation="MKILIGDTCLKTASAIMVNGKKILAYMQSFGEEPHSVMFPKLVD
                     QVLNIAETDFNSLDAVACVYGPGSFTGLRIGLTYLKTLAYTLDIPFYVLNTLDVLANM
                     EEGKRYVVSVPLIEARNNEVFTAMYESEKGIYKRTTPYAAKHIDEVVEDAVSLCAKDD
                     KALILLSGDAQKKHIAKFQEHLTDKVLPYSEERDNVDLSRAHLLISEQCRTDPFTAQP
                     FYLRESGAVRMKKKKQ"
     gene            complement(2531..3019)
                     /gene="tsaE"
                     /locus_tag="PKJ65_03870"
     CDS             complement(2531..3019)
                     /gene="tsaE"
                     /locus_tag="PKJ65_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ41006.1"
                     /translation="MGKKDKTYVIATCNEEDTIRLGKILASAFEDRVIAVCGDLGAGK
                     THFAKGIALGMGIAKHVTSPTFSILNIYRNGDKTFYHMDAYRLTDEESAYNAGIEDIL
                     QPKGITLIEWAENVTDILPDGYVSVNIEKGSDENKRLLYITADRQSVDRMALLIHEDT
                     DR"
BASE COUNT          885 a          783 c          547 g         1143 t
ORIGIN      
        1 tatcacatac cgcagccacc tgattgaatc ctccgtcaat taagaacaga tcgggataat
       61 cgcctctttt catccgtctt tccattactt cacgtaaaga gtcggtatcg gaacgggtgt
      121 tatccccctt tatgatatat cttctgtacc cgcttttata cggtacacct tctttgaata
      181 ccgcttgcga tgcaacggca tcctgtccgg aaagatgcga tatatcaaaa gcttcaatta
      241 catcgggaac cgtttccaga cctaagaaag aagccagaga atagaccata cttgtgcccg
      301 cggaattttt atgctgcccc atgaaaagat tcaatgtcac tacggcattt tcatatgctg
      361 catcggcaag tttcttaagc tttccctttt tcggcacggt aatacttact tttcttccgc
      421 agttgttaaa aagccattgc tccgtatctt ccctgttcat tatttcgtta tacacaacaa
      481 tctctttggg tatattggta tcgtaatact gacgtataaa ctcataccct atatcggtat
      541 cctctatacg ctcctgatgt atatttacgg tctttttgtc cgccactttg ccgtctctga
      601 tgttcatgat aaagaaagac gcaaagtctt tcattcttgc atatgcaata atatccgcgg
      661 aattgcggtc ggtatctaaa atatagttgt cggatattgt tgtcaacaga tttattctgt
      721 cacggtaata tgcggctttt tcaaaattca gattatcgct ttctgcctgc attttacttt
      781 tgtattcatc aataaggtct ctaccgttac ccgataaaaa ccgcaatgcg gcatccaccg
      841 cttctctata cttattctta tcacagtctt ctgaacaggg agcaagacat aagtgcatat
      901 cgtaattgat gcacggccgc ttagccatct tcttacagga acgtaacata aacgcgcttt
      961 gcagcaatgt tacggtgttt ttcttggata ctctgccggg gtaagggccg aaatatttcg
     1021 cattcttatc tgatctgttt cttgtcacat atattccggg aaattgctct tgtgtagtta
     1081 tctttatata cggataagat ttgtcgtctt tcattaagat attgtatttc ggtcgcaaag
     1141 acttgataag atcgctttcg gttaaaaaag cctgcctttc gttatctaat actatgtact
     1201 cgaaagtctc aatattgctt accatctttt tggttttata tgaatgtgcc gacatattgg
     1261 taaagtagga gctcaccctg tttttcagat tgaccgcttt gcctacatat atcgtgttac
     1321 ctttattatc tttcataaga taaactcccg gcttttccgg acaagtcgat aacagataag
     1381 ctattttttc attcaatacg gtcatttaca gttccttcca cataattacg gcatcttcat
     1441 ttgtatctct gtagtactct ttccgtatac cgtctcttac aaaaccgtgt ttttcataca
     1501 gtcttaatgc ggcatcatta tgctccctta cttccaatgt catccgcttt atctccactt
     1561 ctctactgta gtcgataagt gcggacaaca gcaatgttcc tatcccttta ttgcgatacc
     1621 ttttatccac cgcaatgttc atgatgtgtg cttcgtccag cacatgccac atacctatat
     1681 atcccacaac ctcatcatca aagactgccg ctaaatagaa tgacatgggt gttctgcata
     1741 tatcctcata cagcgattca tacgaccagt agtcattgaa ggtttctttt tctatagcga
     1801 atattctgtc cacatcatta atatccgctc tttttatccg tatattcatt gttttttctt
     1861 cttcattctt acggctcccg attctcttaa gtaaaacggt tgcgccgtaa aaggatcggt
     1921 tctgcattgc tctgatataa ggaggtgagc cctactcaaa tccacattat ctctttcttc
     1981 actgtacggt aaaactttat cagtcaaatg ttcctgaaac ttagctatat gttttttctg
     2041 agcatctccg cttaacagta ttaaagcctt atcatccttg gcacataaac ttaccgcatc
     2101 ttcaactacc tcatcaatat gtttggctgc ataaggggta gtccttttat atatgccttt
     2161 ttccgactca tacatggcgg taaaaacttc attgttccga gcttcaatca aaggaacgga
     2221 aacaacatat ctctttcctt cttccatatt tgccagcaca tccaaagtgt ttaaaacata
     2281 aaacggtata tccagagtgt atgccaatgt tttcaaatat gtcagcccta ttcttaaccc
     2341 cgtaaaagag ccgggaccgt atacacatgc aacggcatcc aatgaattaa aatccgtttc
     2401 cgctatattt aagacctgat cgacaagttt agggaacata accgaatgag gctcttctcc
     2461 aaaagactgc atgtatgcaa gtatcttttt tccgttcacc attattgccg aagctgtttt
     2521 taaacaggta tcacctatca gtatcttcat gtataagcaa agccatcctg tctacagatt
     2581 gtctgtccgc cgttatatac agcagtcttt tattctcgtc cgaacccttt tctatattca
     2641 cacttacata cccgtcgggc agtatatccg ttacattctc cgcccattcg ataagcgtaa
     2701 ttccctttgg ttgcagtata tcttcaattc ccgcattata tgcgctttcc tcgtcggtaa
     2761 gcctgtatgc gtccatatga taaaaggtct tgtcaccgtt tctatatata ttcaaaatgc
     2821 tgaatgttgg actggtcaca tgctttgcta ttcccattcc caaagcaatt cccttggcaa
     2881 aatgagtttt accggcacct aaatccccgc acacggctat aaccctgtcc tcaaaagccg
     2941 atgcaagtat cttacccaac cttatcgtat cttcctcatt gcatgtggct attacatatg
     3001 ttttgtcctt ttttcccatg ctatctccca tcgcctattt tataccgaag tgaaaatata
     3061 tacaagaaag aggatttgtt ccgttaagag ggtataattt gcagatattg aaaattatac
     3121 ccccttaacc cggagtttgt taattttctg tatgttgcgc tgtataactt accgtcttaa
     3181 attttagtca acaagtcaca aaaagcctta catatcttct gtcttattcc ttcaccggta
     3241 ttataaactc tgctcaaaca gagaggacta caaaggaaag ttttttagca aatttcataa
     3301 acttgtttgt tcaaggtgta caaataagtt tttctttctt ataggattac ttggagga
//
LOCUS       DAOPWC010000137         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29047_length_3346_cov_3.679429, whole genome shotgun sequence.
ACCESSION   DAOPWC010000137 DAOPWC010000000
VERSION     DAOPWC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29047_length_3346_cov_3.679429"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PKJ65_03875"
     CDS             complement(<1..130)
                     /locus_tag="PKJ65_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ41007.1"
                     /translation="MYRILERKDLNSDVVYMKVDAPAVAKKCEPGQFIILRVDETGE"
     gene            complement(202..1125)
                     /locus_tag="PKJ65_03880"
     CDS             complement(202..1125)
                     /locus_tag="PKJ65_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ41008.1"
                     /translation="MSRTIKALIIGGVFMLLGLVIFLVGYFSNNRNLDSLLEFEIRGT
                     TPLSSKSYDAPQPDSITMISIITKRMPVSVQSHDEQYIRLEYYENADQSFEITQTGGK
                     LIVKKNDLGFFKENVTFNLQSLLVKKKQDNGITVYIPSSYTGELLVSTTFSDISVRNA
                     LAFSGIKKIDFKNYRAGIQMHSLSGNILNLMNTGGIIECSDISFEDITVISTNADVKL
                     NYISSDYTDFLINKSSLQAAGIISDNIKIETVSSQIDMAINAYSVQYSVMIEKSEKSQ
                     INLTESIVENADKRIRIKADDSIISVSFSLD"
     gene            complement(1109..1534)
                     /locus_tag="PKJ65_03885"
     CDS             complement(1109..1534)
                     /locus_tag="PKJ65_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41009.1"
                     /translation="MMVKGRTLLGYDDNPDKRKNLKYTDWLIIIIASSPLWVSLFIAV
                     VVAICVLFAALVLGFTLTGFGSVMFFVYSAFSLADDLWPSVMKMGICLFASGAVIIAV
                     AGSFKGIKVFFVLLKSGYMKIKNMLLLKKLEEQENEQNN"
     gene            complement(1531..2361)
                     /locus_tag="PKJ65_03890"
     CDS             complement(1531..2361)
                     /locus_tag="PKJ65_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ41010.1"
                     /translation="MKKSKYRWIYIVASIIVALVTVFLIFSAERQHNFEAMDPNCSDA
                     KWLINQPVAHRGYYNEMTAPENSLEAFSEAIKKGYAVELDVRLTKDNRIVVFHDKNLK
                     RMTGFDSDVSKTTLEQIKALTLSETSATIPELTEALQLINGNVPVLLEIKSSGLSHNL
                     DKLVYSVIKEYKGSIAVQSFNPLSIRWFAKNAPGIARGILLRGNHKYDIANRFTDNLH
                     MLFSKPNFVAYKYENLVKTDFSQLRRDGLIVLGWTVGENMLNTREFTAHCDNIILEAE
                     "
     gene            2540..>3346
                     /locus_tag="PKJ65_03895"
     CDS             2540..>3346
                     /locus_tag="PKJ65_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ41011.1"
                     /translation="MAKKLKTMDGNQAASYVSYAFTDVAAIYPITPSSPMAENVDEWA
                     AFGQKNMFGQQVKVVELQSEAGAAGTVHGSLQAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPSVFHVSARAVAGHALSIFGDHSDVMSTRQTGWGLLCSGSVQEVQDMAAIAHRST
                     LESRIPFIHFFDGFRTSHEVNNMELVSEDDLRAMIDYNKVLEFRNRALTPENPVVRGT
                     AQNPDVYFQGREAVNKYYNACPEKVQEAMDKFASITGRQYKLFDYVGDADA"
BASE COUNT          989 a          686 c          663 g         1008 t
ORIGIN      
        1 gttctcccgt ttcatctaca cgaagtatta taaattgtcc cggctcgcat ttctttgcaa
       61 cagcgggagc atccactttc atgtagacaa catcactgtt taaatctttt ctttccaata
      121 ttctgtacat taagataccg ccatttcttt taatgtgtaa atgataccac ggcatatgtg
      181 tattttcaat gaagcgaagt gttaatccag agaaaaactt accgaaataa ttgagtcatc
      241 cgctttgatg cgaattctct tatccgcatt ctcgacgata ctctccgtca gatttatctg
      301 cgatttttcc gatttctcaa tcattaccga atattgaaca ctgtatgcgt ttattgccat
      361 atcaatctgc gaagataccg tctcgatttt gatattgtcg gatattatac ctgctgcctg
      421 tagagaactc ttatttatta agaagtcagt atagtcggaa gaaatatagt tcagttttac
      481 atctgcattt gtggatataa cggtgatgtc ttcaaaactt atgtccgagc attctattat
      541 cccgccggta ttcatcaggt tcagaatatt gccggacagt gaatgcatct gtatacccgc
      601 acggtaattt ttaaaatcaa tcttttttat accggaaaag gcaagggcat tcctgacaga
      661 gatatcggaa aaggtagtgc tgactaacaa ttctccggta taagacgagg ggatataaac
      721 ggtgataccg ttatcctgct tctttttcac taataaagac tggagattga acgttacgtt
      781 ttccttgaaa aaccccaggt cgtttttctt gacgataagt ttcccgcccg tttgcgttat
      841 ctcaaatgac tgatctgcgt tttcataata ctccaaccgg atatattgct catcatgaga
      901 ttgaacagag acaggcattc tttttgttat tatacttatc attgtaatac tgtcgggttg
      961 tggggcatca taggattttg atgacagggg agttgttccc cggatttcaa attcaagcag
     1021 actgtccaga ttgcgattgt tggaaaaata accgacaagg aatattacca gtcctaaaag
     1081 cataaatact ccgccgatta tgagggcctt aattgttctg ctcattttcc tgctcctcca
     1141 gttttttcaa taacagcata tttttaatct tcatataacc tgatttcaat aatacgaaaa
     1201 aaacctttat acctttaaaa gatcccgcaa ccgcaattat tacggcgccc gaagcaaaaa
     1261 gacaaattcc cattttcata acggaaggcc acaggtcatc tgccaatgaa aaagcggaat
     1321 aaacaaaaaa catcaccgag ccgaatcctg tcaatgtgaa acctaagacc agtgcggcaa
     1381 ataatacaca tatcgcaaca acaacagcta taaacaaaga cacccataac ggtgatgagg
     1441 caataatgat aatcagccaa tcggtgtact taagattctt ccttttatcc ggattgtcat
     1501 catatcctaa aagggttctt ccctttacca tcattcggcc tccagtataa tattgtcgca
     1561 atgagcggta aattctctag tgttgagcat attctctccc accgtccacc ccagtactat
     1621 gaggccgtcc cttcttaatt gggaaaaatc ggtttttacg aggttctcgt atttatatgc
     1681 cacaaaattt ggtttggaga acaacatgtg aaggttatcc gtaaacctgt ttgcgatatc
     1741 gtatttatgg ttgcctcgca acagtatgcc tctggctata cccggagcat tttttgcaaa
     1801 ccatcttata cttaaaggat tgaaagactg cacggcaata cttcctttat actcctttat
     1861 aaccgaataa acgagcttat ccaaattatg agataatcct gaggatttta tctccagtaa
     1921 tacaggcaca ttaccgttga taagctgcaa ggcttcggtc agttccggta tagtggcgga
     1981 agtttccgac agcgtaaggg ctttaatctg ttccagagtt gtcttactta catcggaatc
     2041 aaaccccgtc attcgtttaa gattcttgtc atgaaagacc acaatgcggt tgtctttcgt
     2101 aagtctcaca tccagctcaa cggcatatcc ttttttaatc gcttccgaaa aagcctccaa
     2161 tgaattctcg ggagcggtca tttcgttata gtatcctctg tgagcaacgg gttggttaat
     2221 gagccatttc gcatcgctgc agttaggatc cattgcttca aagttatgtt gtctctctgc
     2281 cgaaaatatc agaaagaccg ttactaaagc aactattata gaggcaacaa tatatatcca
     2341 cctgtatttt gattttttca tttatttctc ccggcagcct ttgctttctt tgacatgatt
     2401 ataccatagg cgaagtacag tatggaataa ggtgaaattt caaagagtgg agtacaaaag
     2461 attgcgtatg tgtgctacaa tataatgtgg taacaaccaa gttattaatt aatatacatg
     2521 ttaaataaag gagaaacaaa tggcaaagaa acttaaaacg atggatggga accaggcagc
     2581 gtcatatgta tcatatgctt ttaccgatgt tgccgctata taccccataa caccctcgtc
     2641 gcccatggcg gaaaacgtag atgaatgggc ggctttcggt cagaaaaaca tgttcggtca
     2701 gcaagtcaag gttgtcgagc ttcagtcgga agcaggtgca gccggtaccg ttcacgggtc
     2761 attacaggcg ggagctttaa caacaacata tacggcatca cagggattat tactcatgat
     2821 acccaatatg tacaagattg cgggagaact gttaccgtcg gtattccatg tcagcgccag
     2881 agccgtagca ggtcatgcgc tttccatatt cggagatcat tcggatgtta tgtcaacgcg
     2941 gcagacaggc tggggattgt tgtgttcggg ttctgttcag gaagtacaag atatggcagc
     3001 tatagcacac agatcaactc ttgaatcaag aattccgttt attcatttct ttgacgggtt
     3061 cagaacttcg cacgaagtta ataatatgga actggtttct gaagatgatt tacgtgcgat
     3121 gattgattat aataaagtac ttgaattcag aaacagagca ttaacaccgg agaatccggt
     3181 tgtaagggga acagctcaga atcctgatgt atatttccag ggaagagaag ctgttaataa
     3241 atattataat gcttgtccgg aaaaagttca ggaagctatg gataaatttg cttcaattac
     3301 cggaagacag tataaattat ttgattatgt aggcgatgca gatgca
//
LOCUS       DAOPWC010000138         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29458_length_3313_cov_3.206568, whole genome shotgun sequence.
ACCESSION   DAOPWC010000138 DAOPWC010000000
VERSION     DAOPWC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29458_length_3313_cov_3.206568"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKJ65_03900"
     CDS             <1..919
                     /locus_tag="PKJ65_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ41012.1"
                     /translation="RAKGKHIITTNIEHPAVLDTFRYMKKIGYDITLVPVEKNGIVNA
                     SDIEKAIRKDTILISVMFANNEIGTIQPIAEIGAIAKKHNIVFHTDAVQAAGAIPIDV
                     KALNIDLMSVSAHKFHGPKGVGALYIRRGIVIDNLLHGGGQERTRRAATENVCGIVGL
                     AEALKLSVENMEKNNAHIKAIRDAVKAGIEAGIPYIIYNGDETNRLVNNINFCFKFIE
                     GEALILLLDMKGICASSGSACASKSLDPSHVLLALGLPHEIAHGSLRLTFGKDSSMND
                     VGYIVDTVAETVDRLRQMSPLYESFIKEH"
     gene            981..1409
                     /gene="nifU"
                     /locus_tag="PKJ65_03905"
     CDS             981..1409
                     /gene="nifU"
                     /locus_tag="PKJ65_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNZ41013.1"
                     /translation="MYSAKVMDHFTNPRNVGVLADANAVGQVGNAKCGDIMKIYMKIE
                     DNIIKDVSFMTFGCGAAIATSSMATELVKGKTVEEALTLTNKAVMDALDGLPPVKVHC
                     SLLAEEAIKAAINDYKKSIGEQVTQDNFACNTCCHNKKCE"
     gene            1419..2867
                     /locus_tag="PKJ65_03910"
     CDS             1419..2867
                     /locus_tag="PKJ65_03910"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ41014.1"
                     /translation="MVENKDIKDVLFYERKNVWKEISEEQVTSINQFSEGYKKFLDQC
                     KTERECVKYIHNLCLTKENSFISLKSAYKNRQQLHPGDKLYYIEHNKAIILITIGKEG
                     LSQGVKIIGSHIDAPRLDLKPLPIFEDTELGFLKTHYYGGIRKYQWVSVPLALHGIIY
                     KADGTEVEINIGENETDPIFVIPDLLPHLSAEQNKLPLKDAIPGESLNLLTGSVPNVD
                     TEVTDKVKATILQLLNNKYGITEKDFINAEIEAVPAMKARDVGIDKSFVGGYGQDDRV
                     CAYTSLMAFFDASNTINDKTCYCYFSDKEEIGSYGNTGAQCDTLTYVTELIAELLAPS
                     EKNDKQSINVKNVFRESVMLSADVAAAFDPNHKTVFEANNAAYINRGIALSKYNGAGG
                     KGGTSDANAEFVNAVTKLFDQSDDIVWQIAELGKIDAGGGGTISKFGAHLGINVLDAG
                     VPVLSMHSPYEITSKADIYMTYKAFKAFYENL"
     gene            complement(2943..>3313)
                     /locus_tag="PKJ65_03915"
     CDS             complement(2943..>3313)
                     /locus_tag="PKJ65_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ41015.1"
                     /translation="SIANSTADKDEIENVLRLVNIWDEICSLREGLNTNINDNVISVG
                     QRQRICIAMALLRKKDILILDEPVSALDIENSHNIEMILKRISENSTVIASSHEKQLL
                     KTFDEIITFKNGRIVERIIN"
BASE COUNT         1090 a          597 c          726 g          900 t
ORIGIN      
        1 cagagctaaa ggtaaacata tcataaccac aaacatagag catcccgcag ttttggatac
       61 tttccggtat atgaaaaaga tcggttatga cattacgttg gtccccgtag agaaaaacgg
      121 tattgtcaat gcttcggata ttgaaaaagc aatacgcaaa gataccatat taatctccgt
      181 aatgtttgca aacaatgaaa taggaacaat acagccaata gccgagatag gagcaatagc
      241 aaaaaagcat aacatagtgt ttcataccga tgccgttcag gctgcagggg cgatacctat
      301 tgatgtaaaa gcattgaata ttgaccttat gtccgtttcc gctcataaat ttcacggacc
      361 taaaggtgtc ggagcacttt acataagaag aggtattgtt attgataatc ttttgcacgg
      421 cggagggcag gagagaacca gaagagcggc taccgagaat gtctgcggga tagtaggcct
      481 ggcggaagca ctgaaactgt cggtggaaaa tatggaaaag aacaatgctc atataaaggc
      541 aataagagat gcggtaaaag ccggtattga agccggaata ccgtatatta tctacaacgg
      601 tgatgaaaca aacagacttg ttaataacat aaatttctgt tttaagttca ttgaagggga
      661 agcattgata cttcttctgg atatgaaggg catatgtgct tcttccggct cggcatgtgc
      721 atcaaaatcg ttggatcctt cacatgtgct tttagcactg ggattaccgc acgagatcgc
      781 acacggttct ttgcgattga cattcggcaa agacagttcc atgaacgatg tgggctatat
      841 agttgatacg gttgctgaaa cggtggacag attgagacaa atgtcgcctt tgtatgaatc
      901 tttcataaaa gaacactgat gataactatt atagctgcca tattatgcta taatataaaa
      961 aatgaaagga gtgcatgtta atgtacagcg ctaaagtaat ggatcatttc acaaatcccc
     1021 gtaatgtggg agtgcttgcc gatgccaatg cggtaggaca ggtaggcaat gccaaatgcg
     1081 gagatattat gaaaatatac atgaaaatag aagataatat tattaaagat gtcagtttca
     1141 tgactttcgg atgcggagcg gcaattgcaa caagcagtat ggctaccgag ctggttaaag
     1201 gcaaaacagt ggaagaagcc ttgacattaa ccaataaggc ggttatggac gcactggacg
     1261 ggttaccgcc ggttaaggtg cattgttcac tgcttgcgga agaagcgata aaggcggcta
     1321 tcaatgacta caaaaagagt atcggggaac aggtaacgca agataacttt gcctgtaaca
     1381 cctgttgcca taataaaaag tgcgaatagg agagcaaaat ggtagagaat aaagatatta
     1441 aagatgtgct tttttatgaa agaaagaatg tctggaagga aataagcgaa gaacaggtta
     1501 cttccataaa ccaattttcc gaaggatata aaaagttttt agaccagtgt aaaaccgaaa
     1561 gagaatgtgt gaaatacata cataatcttt gtttaacaaa agaaaacagc tttatatccc
     1621 tgaaaagtgc ctacaaaaac agacagcaac tgcaccccgg agataagctt tattacatcg
     1681 aacacaataa ggcaattatc ctgattacaa tcggtaagga aggactttct cagggagtta
     1741 aaataatagg ttcacacata gatgctccca gactggattt aaaacctctc ccgatatttg
     1801 aagataccga gcttggtttt cttaaaactc actattacgg aggtataaga aaatatcagt
     1861 gggtatcggt tcctctggca cttcacggga taatttataa ggctgacggc accgaagtcg
     1921 agataaacat aggtgagaat gaaaccgacc ctatcttcgt cattcccgat ttacttccgc
     1981 atctttcggc cgaacagaat aaattgcctt taaaggatgc tataccggga gaaagtctga
     2041 atcttcttac aggttcggtt cccaatgtcg acacggaggt cactgacaag gtcaaagcga
     2101 ccattttaca gttgcttaat aacaagtacg gtataaccga aaaagacttc ataaacgccg
     2161 aaatagaggc ggttcccgcc atgaaggcaa gagatgtggg tatagataag tcttttgtag
     2221 gcggatacgg acaggatgac agggtatgtg catatacatc attgatggca ttctttgacg
     2281 caagcaacac tattaatgat aagacctgct attgctactt ctcggataaa gaagagatag
     2341 gttcttacgg aaataccgga gctcagtgtg acacattaac ttatgtaacc gaactgattg
     2401 cggaattact ggctccttcg gagaagaatg acaagcaaag tataaatgtt aaaaatgttt
     2461 tcagagaatc ggttatgctt tcagccgatg tggctgccgc tttcgacccc aaccataaaa
     2521 ccgtatttga agccaataac gcggcatata tcaacagggg aatcgcactt tccaaatata
     2581 acggtgcagg cggtaaaggc ggcacaagcg atgcgaatgc ggagtttgtc aatgccgtaa
     2641 ccaaattatt cgatcaaagt gacgatatag tctggcagat tgcggaactg ggtaagattg
     2701 atgcgggcgg gggagggacc atctccaaat tcggtgctca cttaggtatc aatgtgttag
     2761 atgccggagt acctgtatta tccatgcatt caccttatga gattacaagc aaagccgata
     2821 tctatatgac atataaagcg tttaaagcat tttacgagaa tctctgataa taaggttgac
     2881 agaaagaaag taaaagacct ttcggggtct tttttttgca tcatattttg taaatatctg
     2941 catcagttta ttatcctttc gactattcgg ccgtttttaa aagttattat ttcatcaaat
     3001 gtttttagta attgtttttc atgagatgat gctatgactg ttgaattttc ggaaattctt
     3061 tttaatatca tttcgatatt atggctgttt tctatatcca aagccgatac aggctcgtca
     3121 agaataagaa tatctttttt tcttaataaa gccatcgcaa tacaaatacg ctgtcgctga
     3181 cccacagaaa taacgttatc attgatatta gtgtttaaac cttctcttaa actgcaaatt
     3241 tcatcccata tatttacaag tcgtaaaaca ttttcgattt catctttgtc tgctgttgaa
     3301 tttgcaatag aaa
//
LOCUS       DAOPWC010000139         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29508_length_3309_cov_2.745851, whole genome shotgun sequence.
ACCESSION   DAOPWC010000139 DAOPWC010000000
VERSION     DAOPWC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29508_length_3309_cov_2.745851"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..845)
                     /locus_tag="PKJ65_03920"
     CDS             complement(60..845)
                     /locus_tag="PKJ65_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNZ41016.1"
                     /translation="MNILIMTDLEGISGIYASDQVLPGERRFNEGREFMTADINACAR
                     GLKRAGAKKVYVRDCHGGSYTLIQERISEDVDYLILGDVGQDRFLGLDDCQAVILLGY
                     HAMAGASSAILEHTMSSSYVQNYWINGKKAGEFAIDAGIAGDMGKPVIMVSGDDKLCT
                     EAKSLVPDIYCAEVKKGVTSFGGILLPLEKARKLIEDTAYEAYKNIDKIKPLVFKKPI
                     RLRVERVERGRLPLLASKPYMKIIDARTYEVKADTMNEALFRI"
     gene            complement(973..1227)
                     /gene="rpsR"
                     /locus_tag="PKJ65_03925"
     CDS             complement(973..1227)
                     /gene="rpsR"
                     /locus_tag="PKJ65_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNZ41017.1"
                     /translation="MDRMDKEDRKDFKFRRPKKKVCQFCADKNTIDYKDLNTVKRYIS
                     ERGKILPRRVTGTCAKHQRDVTIAVKRARQIALLPYVTDN"
     gene            complement(1258..1668)
                     /gene="ssb"
                     /locus_tag="PKJ65_03930"
     CDS             complement(1258..1668)
                     /gene="ssb"
                     /locus_tag="PKJ65_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ41018.1"
                     /translation="MNKVILMGRLTRDPELRATATGTPVASFSLAVNRRFAAKDAQVQ
                     ADFFNVTAWSKQGEFVNNYYRKGMQVVVIGRLQNRTWEDQEGKKRYATDVILEETYFA
                     EGKRDAGEGSTGSNAGEDMDFTHIDISEDDALPF"
     gene            complement(1678..1959)
                     /gene="rpsF"
                     /locus_tag="PKJ65_03935"
     CDS             complement(1678..1959)
                     /gene="rpsF"
                     /locus_tag="PKJ65_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNZ41019.1"
                     /translation="MNQYETIFIVDCTMEEEVQKSIVEKMVQQMTDAGCVVEKVDEWG
                     RKKLAYPINYKTDGYYVLVTFQAAPDFIAELERLYRITEGILKGQTIRL"
     gene            complement(2134..3078)
                     /locus_tag="PKJ65_03940"
     CDS             complement(2134..3078)
                     /locus_tag="PKJ65_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNZ41020.1"
                     /translation="MLYPLKFEPVYKDYLWGGNNLTKLNKKLIYPITAESWEISANKD
                     GMSIVSNGNLAGNTLLDIFQRYPKKLCGKREVKQFPLLIKLLDACSALSVQVHPDDAY
                     AYANENGALGKNEMWYVVDAKPDARLVYGLKKGVTEEQFVKAVRKNNIGSTLNYVSVK
                     KGDWIYIPAGLVHAIGAGIIIIEIQQNSNTTYRVYDFDRVGADGKKRPLHIEKALDVI
                     NFDAAEMQINEIVSVSGVDYLAYNKYFAVEYIASEGQYKSDTLNLSFHTYTVIEGGFE
                     VEGVSVGLGESVLIPACMKKYTLKGGFRAVKSFVPDIK"
     gene            3164..3240
                     /locus_tag="PKJ65_03945"
     tRNA            3164..3240
                     /locus_tag="PKJ65_03945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3198..3200,aa:Met,seq:cat)
BASE COUNT          844 a          851 c          570 g         1044 t
ORIGIN      
        1 gcctaaggtt catcgcatat attaacatta taacacaatc atgtcaaata tctttttggt
       61 cagatgcgga acaatgcctc gttcatagta tccgctttga cctcataggt acgggcatcg
      121 attatcttca tataaggctt ggatgccaaa agcggcaatc tgccccgctc cacacgctcc
      181 acacgcaatc ttatgggctt tttaaatacc agcggtttaa tcttatcaat gtttttatat
      241 gcctcatatg ccgtatcttc tatgagctta cgagcctttt caagcggcaa caatattcct
      301 ccgaaagagg tgacaccttt ttttacttcg gcacagtata tatcgggaac cagcgacttg
      361 gcttcggtgc acaatttatc atctcccgac accattatta caggttttcc catatccccc
      421 gctataccgg catctatcgc gaactctccc gcctttttac cgtttatcca gtaattctga
      481 acatagctgc tggacatggt gtgttctaaa atagctgacg atgctcccgc cattgcgtga
      541 tatcccagta atataaccgc ctgacagtcg tccagtccca aaaagcgatc ctgtccgaca
      601 tcgcccagta tcagatagtc tacatcctcg gatatccgct cctggataag cgtatagctg
      661 ccgccgtgac agtcgcgtac atatactttc tttgcacctg cgcgtttaag tccgcgtgca
      721 caggcattga tatccgccgt cataaattca cgtccttcgt taaatcttct ctctcccggt
      781 aatacctgat cggatgcata gataccgctg ataccttcaa gatcggtcat tataagaatg
      841 ttcatgatat ggctcctttt tattgctttt atccattata tcatccataa agacaaagtg
      901 taatgtccta tccgccgatg tgatgaaatt gcacataagg gcgaaaaaaa agagcttgcg
      961 ctctctttaa tctcagttat cggtaacata cggcagtaag gcaatctgtc ttgctctctt
     1021 aaccgcgata gtcacatccc tttgatgctt tgcacatgtg cccgtaactc ttctgggaag
     1081 aatctttcct ctttcggaaa tatatctttt caccgtattc aagtctttat aatctatagt
     1141 atttttatcg gcacaaaact ggcaaacctt cttcttgggc cttctgaatt taaaatcttt
     1201 cctatcttct ttatccatgc gatccattat atgtccgctc ctttcatcct tgtcttatta
     1261 aaacggcaaa gcatcatctt cggagatgtc gatatgtgtg aaatccatat cctctcccgc
     1321 attgcttccg gtgctgcctt cgcccgcgtc tctcttacct tccgcaaaat acgtttcttc
     1381 gagaattaca tcggtagcat atctcttctt accttcctga tcttcccaag tacgattctg
     1441 cagtcttcct atgacgacaa cctgcatacc cttacgataa tagttattaa caaattcgcc
     1501 ctgcttggac cacgcggtta cgttaaagaa atccgcctgt acctgagcat ccttagccgc
     1561 aaatcttctg tttacagcca atgaaaagga tgctaccgga gtgccggttg ccgttgctct
     1621 taattcaggg tctcttgtaa gtcgtcccat taatatgacc ttattcatat ttccctccta
     1681 taacctgatt gtctggcctt ttaaaatgcc ttccgttatc ctgtacaatc gctcaagttc
     1741 cgcaatgaag tccggcgcag cctgaaatgt caccagcaca taatagccgt ctgtcttgta
     1801 atttatcgga taagcaagct tctttcttcc ccactcatca accttttcca ctacacagcc
     1861 ggcgtcagtc atctgctgaa ccatcttttc cactatgctt ttttgaactt cttcttccat
     1921 tgtacagtca acaataaaaa ttgtttcata ctgattcata tggtttacct ccttccggtc
     1981 taatcggccc ctttttatcg gggcaaggaa tcaatgcggt agttattgta ctataccgca
     2041 acatcttatt caatagttat tttacataaa tgcctccttt ccgataggaa aaaagacaat
     2101 atcatatgcc gctgtatcaa ttaacggaca aatctatttt atgtccggaa caaaactttt
     2161 taccgctctg aagccgcctt ttaaggtata tttcttcatg catgcgggaa tcagtaccga
     2221 ttctcccaat ccgaccgaca cgccttccac ctcaaaacct ccctcaatta cggtatatgt
     2281 atggaaggac aaattcagcg tatcactctt gtattgccct tccgaagcta tgtactccac
     2341 tgcaaaatat ttattgtatg ccagataatc cacacccgaa acactcacta tttcattaat
     2401 ctgcatctcg gcagcgtcaa aattaatgac atccaatgct ttctctatat gaagaggccg
     2461 cttcttgccg tcggctccca ctctgtcaaa atcatatacc ctgtatgttg tattggagtt
     2521 ctgctgtatt tctattatta taattcccgc tcctattgca tgaaccaacc cggcaggaat
     2581 ataaatccag tcgcctttct ttacggaaac ataatttaat gtgctcccta tattattctt
     2641 cctgacggct tttacgaact gctcctcggt aacacctttt ttcagcccgt atacaagtct
     2701 tgcatcgggc tttgcatcca ccacatacca catctcattt ttccccagag caccgttttc
     2761 attggcataa gcataagcat catcgggatg tacctgcacc gataatgccg agcaggcatc
     2821 taaaagttta atgagcaaag gaaattgttt tacttctctt ttaccgcaca gtttcttggg
     2881 atagcgttga aaaatgtcca aaagagtgtt gcccgccaga ttaccgttgg aaacaatact
     2941 cattccgtct ttattggcgg atatttccca actctccgct gtgatagggt atataagttt
     3001 tttgttgagt ttagttaaat tattgccgcc ccataaatag tctttgtata cgggctcaaa
     3061 ctttaaagga taaagcatac aaacctccat atacaatgag tatatattaa ttcctgttga
     3121 caaacaatat gccttttgtt agaattaccc gtgcgatatt aacggcgaag tagctcagtt
     3181 ggctagagca accggttcat acccggtagg tcgttggttc gaatctgacc ttcgctacca
     3241 tcgctttttt tatacggccc attggtcaag cggttaagac atcgccctct cacggcggta
     3301 acaggggtt
//
LOCUS       DAOPWC010000140         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29524_length_3307_cov_3.112854, whole genome shotgun sequence.
ACCESSION   DAOPWC010000140 DAOPWC010000000
VERSION     DAOPWC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29524_length_3307_cov_3.112854"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="rpmE"
                     /locus_tag="PKJ65_03950"
     CDS             <1..140
                     /gene="rpmE"
                     /locus_tag="PKJ65_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNZ41021.1"
                     /translation="FEIGSTRKDIKVEICSNCHPYFTGKQKFVDTGGRVDKFKKKYGI
                     S"
     gene            190..1134
                     /locus_tag="PKJ65_03955"
     CDS             190..1134
                     /locus_tag="PKJ65_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNZ41022.1"
                     /translation="MRKTSIGGSALLEGIMMIGPHKKAIAVRKPDGDIVLDVENLPKK
                     WKIAKIPVLRGAYSLIYQMVLSIKALSYAASFYDIEEEDNDKADGHNEQDKEQSDEKK
                     AETKSSDGELSKTAIFFAVLLSVAITVGLFILLPNFVIGLLKIESAVINNLLEGVLRI
                     AIFIGYLAAVTLMKDIKRVWMYHGAEHKTINCYEANEELTVENVMKYPTSNRRCGTSF
                     MFLVMAVSIIVFSFVGWHSVILNMVFRILLLPLVAGLAYELVKVAGRYDNVFSKIISA
                     PGMLFQKLTAKEPDEGMVECAIMAFNAVVPEDPEEAKW"
     gene            1128..1949
                     /gene="prmC"
                     /locus_tag="PKJ65_03960"
     CDS             1128..1949
                     /gene="prmC"
                     /locus_tag="PKJ65_03960"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ41023.1"
                     /translation="MVISQLIGYCSEIFKDKKEMYFFLEEVFQKDISYFYAHPDEQVE
                     PDKYRLLLEYAERRGKNQPFEYILNKACFYGNSFYVDSHVLIPRNDTEILVEKAIAIG
                     KTMESPRILDLACGSGCAGISIAKELAQSKVIMSDISEKCVEIANKNILINKISNCSA
                     VQSDLFNKIEGQFDIIVSNPPYIPLHEYVALDKQVLEYEPKLALLAGEEGTEYYELIS
                     ADAMKYLQKGGYILYEVGYNQASRVCDILKKYRYTDINVHKDLHNTDRIVIARSE"
     gene            1971..2765
                     /gene="mazG"
                     /locus_tag="PKJ65_03965"
     CDS             1971..2765
                     /gene="mazG"
                     /locus_tag="PKJ65_03965"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNZ41024.1"
                     /translation="MDKHDKKYDFNDLVEIMAKLRSDSGCPWDKEQTHESITTCLIEE
                     AYETCDAIIDNDIEKMTEELGDVLLQVVFHAQIGKENGTFDLSDITDGICRKLIFRHP
                     HIFGDKKMDTAEQVLESWERIKIKEKGYKSEADSMSNIPKTFPALMRANKIQSKAERV
                     GFDWPDVWGAFDKVYEEAHELKRAIETEDTEKQKEEAGDLLFAVVNVCRFLDIDPEQS
                     LDASSDKFIKRFSHVENSCNDKGQYMKEMTLEQLDKYWNEAKNENR"
     gene            2752..2994
                     /locus_tag="PKJ65_03970"
     CDS             2752..2994
                     /locus_tag="PKJ65_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNZ41025.1"
                     /translation="MRIDKFLKVSRLIKRRTVANDACEAQKIFANGKAVKPSYQVKKG
                     DILEIKFVNSSVKVRVTDISDNVKKGDASSLYEIVE"
BASE COUNT         1127 a          524 c          797 g          859 t
ORIGIN      
        1 aatttgaaat aggttcaaca aggaaggata tcaaagtgga aatttgttca aattgtcatc
       61 cgtatttcac gggcaaacag aaatttgtgg ataccggcgg aagagtggac aaattcaaaa
      121 agaaatacgg tatttcgtaa tttaaaggaa aggcgggttg tctgataaac agcccgattt
      181 ttttataata tgagaaaaac atcaatcggc ggttcggcat tactggaagg cataatgatg
      241 ataggaccgc ataaaaaagc aatagcggtc agaaaacctg acggtgatat agtactggat
      301 gtggagaatc ttcccaaaaa gtggaagata gcaaaaatac ccgtattaag aggggcttat
      361 tcactgatat atcagatggt cttaagcata aaggcattgt cttatgcggc ttctttttat
      421 gatattgaag aagaggataa tgataaagca gatgggcata atgagcaaga taaagagcaa
      481 tcggatgaga agaaagcgga aacaaaatca tcggacggtg agttgagtaa gacagctatc
      541 ttctttgctg ttctcttatc ggtggcaata acggtcggtt tgtttattct tctgcccaat
      601 ttcgtgatag gtcttcttaa gatagagagt gccgttatta ataatttact ggaaggcgta
      661 ttgagaattg ccatttttat cggctacctc gcagccgtca ccttaatgaa ggatataaaa
      721 agggtctgga tgtatcacgg tgcggaacat aagaccataa actgctatga agcaaatgag
      781 gaacttaccg tggaaaatgt tatgaagtat cctacaagca accgcagatg cgggacaagt
      841 tttatgtttc tggtcatggc ggtgtccata attgtttttt cttttgtggg atggcattcg
      901 gttattctca acatggtttt caggatactg ttgcttcctc tggtagcggg attggcatat
      961 gagcttgtta aagtagcggg cagatatgat aatgtttttt ccaagattat atccgctccc
     1021 ggtatgttgt ttcagaagct taccgcaaag gagccggatg aaggcatggt ggaatgtgcc
     1081 ataatggcat tcaatgcggt agtaccggaa gatccggaag aagcgaaatg gtaatttcac
     1141 aattaatcgg ttattgttcg gaaatattca aggataaaaa ggaaatgtat ttttttcttg
     1201 aagaagtatt ccaaaaggat atttcctatt tttacgcaca tcccgatgaa caagtggagc
     1261 ctgataagta tcggttgctg cttgaatatg ccgaaagaag agggaaaaac cagcctttcg
     1321 aatatatact gaacaaagcc tgcttttacg gtaatagttt ttatgtggat tcacatgtcc
     1381 ttatacccag aaacgatacg gaaatacttg tggagaaagc gatagccata ggtaaaacca
     1441 tggaatcacc ccggattctg gatctggcat gcggaagcgg ctgtgcgggt atatccatag
     1501 caaaggaact tgcacaatca aaagtaatta tgagcgatat atcggaaaag tgtgttgaga
     1561 ttgcgaataa gaatatattg attaataaaa taagtaactg ttcggcggta caaagcgacc
     1621 tttttaataa aattgaaggg cagttcgata taatagtatc caatcctccg tatattccgt
     1681 tacatgaata tgtcgcattg gacaaacagg tgctggagta tgagcccaag cttgcactgc
     1741 tggcaggtga ggagggaacg gagtactatg aattgatatc cgccgatgcg atgaagtatc
     1801 tgcaaaaagg cggatatatc ctatacgaag taggatacaa tcaggcatca cgggtgtgcg
     1861 atatactgaa aaaataccga tataccgata ttaatgtaca caaagattta cataatactg
     1921 atagaatagt aatagcaaga agcgaatgac gagccggaag gatgtaaaag atggataaac
     1981 acgataaaaa atacgatttt aacgaccttg tggaaataat ggcaaaactg agaagcgata
     2041 gcggatgtcc ctgggataaa gaacagacac atgagtccat tacaacatgc ctgattgaag
     2101 aagcttatga gacatgcgat gcaatcattg ataatgacat agaaaagatg actgaggaac
     2161 tgggagatgt gcttcttcag gtggtatttc atgctcaaat aggtaaagaa aacggaacat
     2221 ttgatttatc ggacattacc gacggtattt gcagaaaact cattttccgc catccgcata
     2281 tattcggtga caaaaagatg gatacagcgg agcaggtact ggaaagttgg gaaagaatta
     2341 agataaaaga aaaaggctat aagagcgaag cggacagcat gagcaacatt cccaagacct
     2401 ttcccgcact tatgagagcc aataaaatac agagtaaagc cgagagagtg ggatttgact
     2461 ggccggatgt ttggggagct ttcgataagg tatatgagga agcccatgag ttaaaacggg
     2521 cgattgaaac ggaagatacc gaaaagcaga aggaagaagc cggagatttg ctttttgccg
     2581 ttgtcaatgt ctgcagattt ttggatatag accccgaaca aagtctggat gcttcgtcgg
     2641 ataagtttat aaagcgtttt tcgcatgtgg aaaacagctg caacgataaa ggtcagtata
     2701 tgaaagagat gactttggag cagctggaca agtactggaa cgaggctaaa aatgagaata
     2761 gataagtttc ttaaagtaag ccgtctcata aagagaagaa ccgttgctaa tgatgcatgt
     2821 gaagcacaga aaatattcgc aaacggtaaa gcggtcaaac cttcttatca ggttaaaaaa
     2881 ggtgatattt tagaaattaa atttgtaaac agttcggtaa aggtcagagt gaccgatata
     2941 tcggataatg ttaaaaaagg tgatgcttcg tcactatacg aaatagtaga gtaaaatatt
     3001 tgtaaatata taaataaggc gattaccggt tctttacaac tctccgtgaa gatgaaataa
     3061 taaaacggtg ttaaacaatg gaaatcggtc ggatacatat agcgtataat gaaaaaccgt
     3121 aatatcatat tgtcggagga taaaatgaga gataaataca cgcacagaaa attcttaatc
     3181 ccgggacgac cgacgctctg cgtaatgatt aaatcccgcg tgacatcgga aataatacgt
     3241 gatataaggc tcgcactgga agaaggatgc gatgctttcg gtcttcagat ggaatcaatg
     3301 ttgccgg
//
LOCUS       DAOPWC010000141         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29613_length_3299_cov_3.465783, whole genome shotgun sequence.
ACCESSION   DAOPWC010000141 DAOPWC010000000
VERSION     DAOPWC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29613_length_3299_cov_3.465783"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2239)
                     /locus_tag="PKJ65_03975"
     CDS             complement(<1..2239)
                     /locus_tag="PKJ65_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ41026.1"
                     /translation="MYCKVCGANIYDGADFCTNCGQRVEKAAVCLNCNAVLNPGAGFC
                     AVCGTPVTQVAPQPKKRVKKPVKNLKKKVLISVTAVVLIVSIVLGIMYVPDIIARNRR
                     YKTSVGRAQKGTVVTVGQDSPVAEAEGITVDFGSFAPEENVKLEINKIKGATDSEMGK
                     ALASYRITADGQEHSEFAGFVRITVPVDMTDYDDIDICATYYDDQSSKWTVLPHETFK
                     EENKLIIYTDHFSDFSVFIISKSTTRFATAALNYKVISNFFESQENSSVYDKLKAGEI
                     PTDSDFITMGLGFLNFTSTAYDQKITMSTIMKNLPLSQVEAMSKTLTKIGFGLVLLKA
                     SYEYFILNSTDAAAQTLLMGLMELGLFAAAGAIGGPVAATVAFTIWTASYLMGLSSAY
                     QRSKDIDTAIKTQEEFVDELYLYCINVRARHTEGGWYYRYNAPVMTSSNSGYISFKAG
                     SDADWIKLFDKLYELNKDNPNAFIDAVAHAINTYHKLWSLTPVNYRGNSWRDDNDPMR
                     YVKYPSLSKADEEIVTATIRENLRKRMAPIIADYKRKIFIKMQVEFEIALEKERDNWN
                     RPISFEVTSDKEEKFYDLYNNAVMRFAVPEELVIHMSEEEIARYSNQWRFPKAEKEEK
                     PRLEATYLGYFIAGMPDTLNIFTDNSSLKQNLPNRTVKFKFSMPDTVITIPDQHPPLS
                     EVAGNYTEGSMFVEYVYVGEHFEEALARAQERADRFAKSIGCETAGEIKVTLGKELVG
                     ETIYTS"
     gene            2324..>3299
                     /locus_tag="PKJ65_03980"
     CDS             2324..>3299
                     /locus_tag="PKJ65_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ41027.1"
                     /translation="MVKYQYIEIKRSYYREGGQMSKYNYSKELKKPISLGNINFENAI
                     IRALIRTGCRLSDLQIKLQKSNKDISEKTVHIPVADNVKIPCTVIEPANIKTSLPAII
                     YFHGGAFVLPLASLMTKNAIYYAEKLQCRVFLPRYRLAPEHPFPTPLYDCYNAFNHLT
                     ENADTYFIDKDNILIYGDSAGGCLAASVCHMLRDNKKHMPNAQILIYPVTDNSVTSKS
                     MDEYKDAAWTKTATVHMWNLYLKNGHTDMLGYAAPLHNSDFCNLPPAYVEAAQMDTLR
                     DEALAYADKLSDAKTPVEIYLAEGAYHGFDKDHTSPLVKKVLKHRIKVM"
BASE COUNT          958 a          743 c          604 g          994 t
ORIGIN      
        1 ttgatgtata tattgtctct cccacaagct cttttccgag agttactttt atttcgccgg
       61 cagtttcaca tcctatactt tttgcaaatc tgtctgccct ttcctgtgct ctcgctaatg
      121 cttcttcaaa atgctcacct acataaacat attcaacaaa catgcttcct tccgtatagt
      181 tgcctgcaac ttcactcaac ggaggatgct gatcgggaat agttattacg gtatcgggca
      241 tgctgaattt aaacttaacc gtccggttcg gaaggttctg tttcagagag ctgttatcgg
      301 taaatatatt taacgtatcg ggcattccgg caataaagta tcctaagtaa gttgcttcca
      361 atctaggctt ttcctctttt tcagctttgg gaaagcgcca ttgattggaa tatctggcta
      421 tttcttcctc cgacatatgt ataaccaact cttcgggtac ggcaaatctc atcactgcat
      481 tattatacaa atcatagaat ttttcttctt tatcggaagt gacctcaaaa gaaatgggtc
      541 tgttccaatt gtctctttcc ttttccaatg ctatttcaaa ctcaacctgc atcttgataa
      601 aaatcttcct tttataatcg gctataatcg gtgccatacg cttccgcagg ttttctctta
      661 tagtagctgt aacaatctct tcgtcggctt tgctcaaaga gggatacttt acatatctca
      721 tcgggtcatt gtcatcccgc caggaattac ctctgtaatt cacaggtgtc aatgaccata
      781 atttgtgata ggtgttaatt gcatgcgcaa cggcatcaat aaaggcgttg ggattgtcct
      841 tgttaagttc atataacttg tcaaaaagtt taatccaatc tgcatcactg cccgctttaa
      901 aggaaatata tcccgagttc gaggaagtca tgacgggtgc attatatctg taataccatc
      961 ctccttccgt gtgtcttgct cttacattta tgcagtataa ataaagttcg tccacaaact
     1021 cttcctgtgt ctttatggcc gtgtcaatat cctttgagcg ttgatacgct gaagataacc
     1081 ccataagata agaagctgtc catattgtga atgcaaccgt tgcagccacc ggtcctccga
     1141 tagcaccggc tgcggcaaac agtccgagtt ccataagacc catcagcaaa gtctgtgcag
     1201 cggcatctgt gctgtttaag ataaaatatt cataacttgc ttttaacaga accagcccga
     1261 atcctatttt agtcagagtc ttactcattg cttctacctg cgatagagga agattcttca
     1321 taattgtgct catggttatc ttctgatcat atgcggtgct ggtaaagttt aagaagccca
     1381 accccattgt tataaaatcg gaatcggtag gtatttcacc cgcttttaat ttgtcataaa
     1441 ccgagctgtt ttcctggctt tcaaaaaagt tgcttataac cttatagtta agtgctgccg
     1501 tagcaaatct tgtagtggat ttggatatta taaatacgga gaaatccgaa aagtgatcgg
     1561 tatagattat aagcttgttc tcttctttga atgtttcatg aggaagaact gtccactttg
     1621 atgattggtc atcataatag gttgcacaga tatctatatc atcataatcg gtcatatcca
     1681 caggaacggt tattcttaca aaaccggcaa attccgaatg ttcctgcccg tcagccgtta
     1741 ttctgtaaga tgccaaagct ttacccattt cactgtccgt tgcgcctttt atcttattta
     1801 tctccagctt aacattttcc tccggcgcaa aagagccgaa atcaacggtt ataccttccg
     1861 cctccgcaac gggactatcc tgcccgacgg tcacaaccgt acctttctga gcccttccga
     1921 cagatgtctt atatctcctg tttcgggcta taatatccgg cacatacatt atccctaaaa
     1981 caatacttac tatcagtaca acggctgtga cggatatcaa tactttcttc ttaagattct
     2041 taacaggttt ttttacacgt ttcttaggct gcggtgcaac ctgagttacg ggagttccgc
     2101 agaccgcgca aaatccggct cccggattta atacagcatt acagttaaga cacacagctg
     2161 ctttttcgac tctttgaccg caattggtgc aaaaatccgc accatcataa atgtttgccc
     2221 cacaaacttt acaatacatg attttgtcct cgctttgccc cattatatca taaaaacaat
     2281 acgtataaca gatgtatccg acaagcatat ttgcgtatgc tttatggtaa aatatcagta
     2341 tatagagata aagcgtagtt actaccggga aggcggacaa atgagtaaat acaactacag
     2401 caaagaatta aaaaaaccga taagtttggg aaacattaac tttgaaaatg ccattatccg
     2461 cgctctaata agaaccggtt gtcgcttatc cgatttacag ataaagttgc aaaagagcaa
     2521 taaagatatt tccgaaaaaa ccgttcatat acctgttgca gacaatgtaa aaataccctg
     2581 caccgttatt gaaccggcaa atataaaaac aagcttaccg gcaataatat attttcacgg
     2641 aggcgcattc gtattgccgt tagcaagcct gatgactaaa aatgccatat actatgctga
     2701 aaaattacaa tgcagagtgt ttctgccccg ttacaggctt gcaccggaac atcccttccc
     2761 tactccttta tatgactgct acaatgcatt caaccattta acagaaaatg cagacaccta
     2821 ttttatagac aaagacaata tactcatata cggagacagc gcaggcggtt gtcttgcagc
     2881 ttcggtatgt catatgttgc gtgataacaa aaaacatatg cccaatgcac agatacttat
     2941 ctatcccgta acggacaatt ccgttacatc aaaatctatg gacgaatata aagatgcggc
     3001 ttggacaaaa accgctaccg tccatatgtg gaatctgtac ctgaaaaacg gacatactga
     3061 tatgctcggg tatgcggcac ctttacacaa ttcggatttc tgtaatcttc ctcccgctta
     3121 tgtcgaagcg gcacaaatgg atacattgag agatgaagcg ctggcttatg cggataaact
     3181 tagcgatgca aaaacaccgg ttgaaatata tttagcagaa ggtgcatacc acggatttga
     3241 taaggatcat acctcaccgt tggtaaagaa agtactgaaa caccgaataa aggtaatgc
//
LOCUS       DAOPWC010000142         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29681_length_3293_cov_3.257566, whole genome shotgun sequence.
ACCESSION   DAOPWC010000142 DAOPWC010000000
VERSION     DAOPWC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29681_length_3293_cov_3.257566"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..669
                     /locus_tag="PKJ65_03985"
     CDS             67..669
                     /locus_tag="PKJ65_03985"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41028.1"
                     /translation="MANTFRNIKKKKDIFIFILAYFFYIDGVYTIITMATIYGTELGL
                     DSVSMVLALLLTQFIAFPCAIIAGILGTKYGTLKIIKIFIGIYMFVAIFGFFLKSEWQ
                     FWVLAAVVGMAQGGIQSLSRGFFGRIIPKEESNEYFGFFDIFGKLADIMGPLLMGLFG
                     IITGHSRYGILSLILLFIIGFVLLSRLPKGFGEVKSVPEE"
     gene            924..1334
                     /locus_tag="PKJ65_03990"
     CDS             924..1334
                     /locus_tag="PKJ65_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41029.1"
                     /translation="MKLKRILSLVMVLVLLTGLCLSLAGCAEPETATYTITNTTPNTV
                     TLKWIRKVKIQQTWLDVKPGEVLEITMDIDKKQPNVLSVFFGGFKVGRDVITPENGFV
                     DGGKYYIIIFRTPGASKDEYKLVPDDGTYVKPAQ"
     gene            1663..2712
                     /locus_tag="PKJ65_03995"
     CDS             1663..2712
                     /locus_tag="PKJ65_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41030.1"
                     /translation="MEYKIGIIGYGGMASWHHKNSVKVPGLTVTSAFDIDSERLKVAE
                     QNGLKIYDNVDDFLKYGDFSIVLVATPNNFHKYYSVMAMNAKKHVVCEKPVAMSLVEL
                     DDMIAASKKNNVLFTVHQNRRWDRDYKTIKRVLEDNLIGKPYTIESRVHGQNGVMHGW
                     RAYKVAGGGMLFDWGVHLIDQILCMINEKVTEVYCQLFSIKTPEVDDYFKLLLRFESG
                     LSAQIEVGTYCLEKMPRWYVNADHGSVIVKNWECEGKITKASEIVMEWEPEVVQTVGG
                     PTRTMAPRPKETLEELALPKVESQWSDYYINVIAHLNGKEDLIVKHEELRRVMKIIEA
                     AFESDRTHQSIKCLI"
     gene            2726..3058
                     /locus_tag="PKJ65_04000"
     CDS             2726..3058
                     /locus_tag="PKJ65_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HNZ41031.1"
                     /translation="MSAKADIYFMTNQKFFPAEKLYAVKEKLDSMTDEQIQRLMFIQM
                     KDPTTYLLVSIFIGQLGVDRFLMNDIGMGILKLLTGGLCGILWIYDMVTIQDKVRGMN
                     FDAFMNFM"
BASE COUNT         1079 a          513 c          737 g          964 t
ORIGIN      
        1 tcttcttgtt ttattaactt ttaaatttat tttccccaaa atactttaca ctaagcattc
       61 ttaagaatag caaacacatt caggaatatt aaaaagaaga aagatatttt tatatttata
      121 cttgcatatt tcttctacat tgacggagta tatacgatta ttacgatggc taccatatac
      181 ggtacggaat tggggttgga ttcggtgtcc atggtgcttg ctttattgct cactcagttc
      241 atagctttcc cgtgtgcgat tatagccggc attctgggaa caaaatacgg aacactcaag
      301 attataaaga tatttatagg aatatatatg tttgtggcaa tcttcggatt cttccttaag
      361 agtgagtggc agttctgggt actggcggcc gtagtaggaa tggcacaagg gggtatacag
      421 tcactgtcaa gaggcttttt cggcaggatt attcccaaag aagaatccaa cgagtacttc
      481 ggtttcttcg acatattcgg caaactggcg gacataatgg gacctctgct catgggcttg
      541 ttcggtataa taaccggaca ttccagatac ggtatcttat ctttgatcct gctgtttata
      601 ataggatttg tgctgttgtc aaggttgcct aaaggtttcg gtgaagttaa atcagtaccg
      661 gaagagtgag aaatgtgaca ttttgtgaca gataatacag tgaataccga aatattgcat
      721 aaatattcag aaatgaacaa attcaatgag ttatatgcat ataaaatgga tatatctgca
      781 taaacaaact tagtgttatt agaagcaatg ttatttaaac acaacgaatg tcatattttt
      841 catatagtaa gagacgggaa taaatgataa aatacaatag gtggagtctt aaaaaacata
      901 ttaaaaaaag ggggttatat ataatgaaat taaaaagaat tctttctttg gttatggtat
      961 tggtactgtt gacagggtta tgtctttcac ttgcgggatg tgcagagccg gaaacagcca
     1021 catacactat caccaataca actcctaaca cagttacgct caagtggatt aggaaagtaa
     1081 aaattcagca aacatggttg gatgttaaac ccggtgaagt gttggaaata acgatggata
     1141 tcgataaaaa gcagcctaat gttttaagtg tattctttgg cggtttcaaa gtcggcagag
     1201 atgttataac tcctgaaaac ggatttgttg acggcggcaa atactatatc attattttcc
     1261 gtacgcccgg tgcttccaag gatgagtata agcttgttcc tgatgacgga acatacgtga
     1321 aacctgcaca ataaaattga ggtattcaat tggagatatg tgagaaggta ttcggctttg
     1381 agttgacttt caaaacatcg gataaattgt tttcatataa gagtgctgat aaaggcactc
     1441 tttttatgtt agataaatgt gatatcaaaa aagaagatat cgttttgatt tgggatacgg
     1501 atgcgggctt gtcgggatat cttgtgcaaa ggttataaaa gggaataatt ttattatgtg
     1561 cgatatagat tttctggctg tggaatattc acagttatta caggtttttg atgttgacag
     1621 gacttgctat aagtaataat atgaagtgga tggaggtata gtatggaata taaaataggc
     1681 ataatcggat atggcggaat ggcttcatgg catcataaaa attctgtcaa agtgcccggt
     1741 ttgacagtga caagcgcctt tgatattgat tcagagagac ttaaggtagc ggaacaaaac
     1801 ggattaaaaa tatatgataa tgtggatgat ttcctgaaat acggtgattt ttccatagtt
     1861 cttgtggcta cacccaacaa cttccataaa tactattccg ttatggcaat gaatgccaaa
     1921 aagcatgtag tatgtgaaaa accggtagcg atgagtctgg tggaattgga tgacatgata
     1981 gctgcatcaa agaagaataa tgttctgttt accgttcatc agaacagaag atgggacagg
     2041 gattataaga cgataaagag agttcttgag gacaatctta tcggtaaacc ttatacaata
     2101 gagtcaagag tacacggtca aaacggagtt atgcacggat ggagagcata caaagtagcc
     2161 ggtggcggaa tgctctttga ctggggtgtt catcttatag atcagattct gtgcatgatc
     2221 aatgaaaaag tcacggaagt atactgccag cttttcagta tcaaaacacc cgaagtagac
     2281 gactatttta aactgttatt aaggtttgaa tcaggtttat ccgcacaaat cgaagtaggt
     2341 acttactgtc tggaaaaaat gcccagatgg tatgttaatg ccgatcacgg ctcggtaata
     2401 gtaaaaaact gggaatgtga gggtaaaata acaaaagcca gcgagattgt aatggagtgg
     2461 gaaccggaag tagtacagac ggtaggaggc cctaccagaa ccatggcacc gcgtcctaaa
     2521 gaaacactgg aggaacttgc acttccgaaa gtggagagcc aatggagtga ctactatata
     2581 aatgtaattg ctcatcttaa cggaaaagaa gatttaattg ttaagcatga agaactaaga
     2641 agagtaatga agattattga ggcggcattt gaatcagaca gaacacatca gagtattaaa
     2701 tgtttaatat aaaggagaga gaaacatgag cgcaaaagca gatatctatt ttatgactaa
     2761 tcagaagttt tttccggctg agaaactgta tgccgtgaaa gaaaaattgg actccatgac
     2821 cgatgagcaa atccaacgtt tgatgttcat tcagatgaag gatcctacta catacctgtt
     2881 ggtctcgata tttatcggac agttgggtgt agacaggttc ctcatgaacg atatcggaat
     2941 gggaattttg aagttattga ccggaggact ttgcggtata ctgtggatat acgacatggt
     3001 taccatacag gataaagtaa ggggcatgaa cttcgacgca ttcatgaatt ttatgtaata
     3061 cctttcggat tttcaaacgg tattgtctta aaatacgcat acaacaatgt gcatagtatt
     3121 tgtacattgt tttgttaccg gataagactg aaatttcaga atagttaaac ctaaaaggat
     3181 ataactccga ggtggctgag cagtatctta aggcctgtca gaatcagtat tatgcctccc
     3241 gttatttccg caccgtactt gaatctgcac ccgaaaatgc tgcctatata tac
//
LOCUS       DAOPWC010000143         3290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29718_length_3290_cov_3.979289, whole genome shotgun sequence.
ACCESSION   DAOPWC010000143 DAOPWC010000000
VERSION     DAOPWC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29718_length_3290_cov_3.979289"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..968
                     /locus_tag="PKJ65_04005"
     CDS             249..968
                     /locus_tag="PKJ65_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ41032.1"
                     /translation="MDIRLMTYNICSGMSYGENRKRDLAQSAEVIKEYAPDILALNEV
                     HYNIRFSGFVKQAEEINKLLNYEYMYYGKAGAIKEGFQGNALFSRYPVKTVHTVLVDQ
                     PAVLDEDVYYEQRSFIDSVIDIGKDLHVIVTHFGLAEGERKNALDLLIPIFRQHRKSL
                     VFMGDLNLEPHEEQLKSISGILKDTAAISNKKYFTFPSDIPDRKIDYIFVSHDIKVND
                     IVVPLTTASDHLPYIADVFIG"
     gene            complement(958..1758)
                     /locus_tag="PKJ65_04010"
     CDS             complement(958..1758)
                     /locus_tag="PKJ65_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ41033.1"
                     /translation="MNGLYKIADSHTHIYPDKIAMKASKSIGDFYGIPMFYNGTVKEL
                     LENGSGINIKKYLVCSVSTTPQQVMSINDFIISQCLEHEEFVGLAAMHQDFNDVEKEL
                     ERVAEQGLKGIKMHHDFQKVYSDDERFFRTYKKAEEAGMVCLLHAGDDRYDYTRPKRI
                     ISVMEKFPNLKCIAAHFGGYKCWEEAYKTYKGTDNLYFDTSSSLYSLKPDEARDIIRE
                     FGSDRFLFGTDYPMWDAKTELEWFLSLNLTERENSSILYDNFINLFNL"
     gene            complement(1870..2286)
                     /locus_tag="PKJ65_04015"
     CDS             complement(1870..2286)
                     /locus_tag="PKJ65_04015"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ41034.1"
                     /translation="MEIKEQVGKRKTGIILSAVIVAAIAALLIFSKVSITVGEQAITV
                     KAGLERSVIAYEDIVKVEKVSSITVGKRIMGADLIKIYSGTFKNEQYGRYRLYIYKDV
                     SNYIVVEHKEGFAVFNSDTAERTDSLYEDILEKLKR"
     gene            complement(2346..>3290)
                     /locus_tag="PKJ65_04020"
     CDS             complement(2346..>3290)
                     /locus_tag="PKJ65_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ41035.1"
                     /translation="GLNEELVMAIAVGHDLGHSPFGHSGERIIDRITKEYLKTDFWHE
                     KNSLRVIDYSETILNAWGQEENLNLTYAVRDGIVTHCGEIERKQLVPRTEYIELEDIK
                     NASAVSPYTYEGCLVKAADIIAYLGRDIEDALRLRILNSQDISRLDEILKQYKKADEA
                     MNNSTIINSLVTDLCFTSKKEHGICFSDEGFMILKKIKAFNYEYIYKNKRLRKYEEYA
                     ELIIQSLFDVLMSFYKEEDTLEYIKTEYSTIYPELASSFSDRLKKYSIMENRPENLKN
                     KMLYNLSSKEDYIIAVIDYISSMTDMYAKKLFDEITSF"
BASE COUNT          896 a          697 c          575 g         1122 t
ORIGIN      
        1 cttttacttt ttttattacc tgttcgaaat ctttatccaa gtcctgtctg actgaatata
       61 gctgtaaagc aatgggcaga gacataattt ccctccttgt ataccgataa ttgtatcatt
      121 attaacggta agctacaaca tcaaaccttt atgtatttac cgtttaattt gatttaatac
      181 atatgtattt ttgtatataa tggaataagg aggaatacat acggtcaatg ccgtttgtgt
      241 aaggtattat ggatataagg ttgatgacat acaatatctg ttcgggaatg agctacggag
      301 aaaacagaaa aagagactta gcccaaagtg cggaggttat aaaggagtat gcacccgaca
      361 ttctggctct taatgaagtc cattacaata tccggttttc cggatttgta aaacaggccg
      421 aagagataaa taaacttctt aactatgaat atatgtacta cgggaaagcg ggagcgataa
      481 aagaaggttt tcagggtaat gcattgtttt ccagataccc tgttaaaacc gttcataccg
      541 ttcttgtgga ccagcctgcg gttcttgatg aggatgtata ttatgaacag cgttccttca
      601 tagattcggt aattgatata ggtaaagact tacatgttat agttacccat ttcggcctgg
      661 ctgaaggtga gaggaaaaac gcattggatt tacttatacc catcttcagg caacaccgga
      721 aatctcttgt tttcatggga gatctgaacc tggaaccgca tgaagaacaa ttgaagagca
      781 tatcgggtat actgaaagat accgctgcta taagtaacaa gaaatatttc acatttcctt
      841 ccgatatacc ggacagaaaa atcgactata tattcgtatc acatgacatc aaggtaaatg
      901 acattgttgt tccgttaacc acggcatccg atcatcttcc gtatattgca gatgttttca
      961 taggttgaag agatttataa aattatcata aagaatactg ctgttttccc gctcggtaag
     1021 attaagagat aaaaaccatt ccagttcggt ttttgcgtcc cacatgggat aatcggtacc
     1081 gaataaaaat ctgtcacttc cgaattccct tattatatcc cgcgcttcat cgggtttcag
     1141 tgaatacaga ctggaggatg tatcaaaata cagattatcc gtccctttat atgttttgta
     1201 tgcctcttcc cagcatttgt atcccccgaa atgcgccgct atacatttca gattcgggaa
     1261 tttctccatt accgatatta ttcgcttggg ccttgtatag tcatacctgt cgtcgcccgc
     1321 atgtaaaaga cataccatgc ccgcctcttc ggcttttttg tatgttcgaa agaatctttc
     1381 atcatccgaa tatactttct gaaaatcatg gtgcattttt atacccttaa gtccctgctc
     1441 cgctacccgc tccagctctt tttccacatc attaaaatcc tggtgcatgg cagcaagtcc
     1501 tacaaattct tcatgctcca gacactgtga gattatgaag tcatttatac tcataacctg
     1561 ttgcggggtg gtggacaccg agcagacaag atacttctta atatttattc cgcttccgtt
     1621 ttccaggagt tccttcaccg ttccgttgta gaacatagga ataccgtaga agtctccgat
     1681 gctctttgat gccttcatcg ctattttatc gggatatata tgtgtgtgcg agtccgctat
     1741 tttgtataaa ccgttcaaaa aaagccatcc cctttacata atggctttat tttacaactg
     1801 tgagaaataa attcaagtat tatctgttat cctctttact atttactatt cctgattctg
     1861 tttacttact tatcttttta atttttccaa tatgtcttca tacaggctgt cggtccgctc
     1921 tgctgtatct gaattgaaca ccgcaaatcc ttctttatgt tccacaacta tatagttgct
     1981 tacatccttg tatatataca gtctgtatct gccgtattgc tcatttttga atgtcccgct
     2041 gtatattttt atgaggtcgg cacccattat tctttttccc accgttatgg aacttacttt
     2101 ctccacttta actatgtcct cataagctat aactgaccgc tccagaccgg cttttactgt
     2161 tatagcttgt tctccgacgg tgatgctgac tttactgaat attaaaagcg ctgcgattgc
     2221 cgctacaata acagccgata aaattatacc tgttttcctt tttccgactt gttcttttat
     2281 ttccattgtt tcctgttccc tctaatcaat atgtagtaaa ttatgtattt catcaaacag
     2341 tttctttaga atgatgttat ctcatcaaac agtttttttg catacatgtc ggtcatggaa
     2401 gatatatagt ctataaccgc tatgatgtaa tcttccttac ttgaaagatt ataaagcatt
     2461 ttgtttttaa gattctccgg tcggttttcc atgattgaat actttttcag ccggtcggaa
     2521 aaagaagatg ccagttccgg ataaatagtt gaatactcgg tttttatgta ttcgagggta
     2581 tcctcctctt tgtaaaagga catcagcaca tcaaacagcg attggataat aagttcggca
     2641 tattcctcat actttctcaa ccgtttgttt ttgtaaatat actcataatt gaatgcctta
     2701 atttttttaa gtatcatgaa tccttcatcg gaaaaacaga tgccgtgttc ttttttactg
     2761 gtaaagcaca aatcggtcac aaggctgttt attatggtgc tgttgttcat ggcttcatcg
     2821 gcttttttgt actgcttcag tatttcgtca agacgggaaa tatcctgtga attaagtatt
     2881 ctcaacctta aagcatcctc aatatctctg cccagatatg caattatatc cgctgccttt
     2941 acaagacagc cttcataagt atacggtgaa accgcagaag cattcttaat gtcttccagc
     3001 tcaatatatt ccgtccgcgg taccagctgt tttctttcta tttcgccgca atgtgtaact
     3061 attccgtccc tcaccgcata agtcaggttg agattttctt cctgtcccca tgcattcaaa
     3121 atggtttcgc tgtaatcaat aacccttaaa ctgtttttct catgccaaaa atccgtcttc
     3181 aggtattctt ttgttatcct gtctataatt ctttccccgc tgtgaccgaa aggcgagtgt
     3241 cccagatcat gacctacggc aatcgccatg acaagttctt cattaagacc
//
LOCUS       DAOPWC010000144         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_29866_length_3279_cov_4.407878, whole genome shotgun sequence.
ACCESSION   DAOPWC010000144 DAOPWC010000000
VERSION     DAOPWC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29866_length_3279_cov_4.407878"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKJ65_04025"
     CDS             complement(<1..589)
                     /locus_tag="PKJ65_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ41036.1"
                     /translation="MRKNFHVLIKPASSLCNMNCKYCFYDDVSNKRKIKSYGIMSQDT
                     SHKIIDRALQACPEGEITFSFQGGEPTLAKLDFFLDFTEYAQQAKTEKIKYMYSIQTN
                     GLLIDETWCRLFKEHDFLAGVSLDGLKEAHDNLRKDNFQKDTFNRVMNGINLLKKYEV
                     TFNILTVVNKYNVRHPQKLWNIYEKNGFDYIQLIPC"
     gene            complement(712..787)
                     /locus_tag="PKJ65_04030"
     tRNA            complement(712..787)
                     /locus_tag="PKJ65_04030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Thr,seq:tgt)
     gene            complement(792..867)
                     /locus_tag="PKJ65_04035"
     tRNA            complement(792..867)
                     /locus_tag="PKJ65_04035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Val,seq:tac)
     gene            complement(958..2184)
                     /locus_tag="PKJ65_04040"
     CDS             complement(958..2184)
                     /locus_tag="PKJ65_04040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNZ41037.1"
                     /translation="MKSLKELYRIGRGPSSSHTMGPDRAARRFGEEHPDAERFEVTLY
                     GSLAKTGLGHMTDKAVKEALPDKPVKVHIDTVTEDLPHPNTMDFVAIKNNEVADKWRV
                     MSIGGGAIRIEGKPYMDSPDFYKEKNFTEIKKYCEKHHLKLYEYVEQCEGPEIYNYLE
                     DVWHHMKLSIKEGLSATGILHGGLKLQRKARYLYRQRHMDESPSTRENRIVCSYAFAA
                     SEQNAGGGIVVTAPTCGSCGVLPAVLKYMQEKNGYSNRDILRALAAAGVIGNVIKTNA
                     SISGAECGCQAEIGTACSMASAGLAELFGMDLDQIEYAAEVAMEHHLGLTCDPIMGLV
                     QIPCIERNAVAAMRAINSVSLANFLTHTRKISFDMVVDTMYETGRDLFSKYRETSEGG
                     LAIKYSEKKGNKLYTT"
     gene            2507..>3279
                     /locus_tag="PKJ65_04045"
     CDS             2507..>3279
                     /locus_tag="PKJ65_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ41038.1"
                     /translation="MKNKSVIKTINIIVPIFILGLIIFNSTIEALVLAVFYFIYRIII
                     GLSKIYSYIAIKYYVKGNLKKARKYYKKAYEVKVKEPPIVASYAYLLILLGEYDQAEQ
                     VLKALESMKPVGKAATSLMLCNAVLMWKRDKNLIAALAHVESMDETMRNQWYYNVYAK
                     LCIFSGNIGKAKEAALKGYEYLESNPVAVENLLIVHCINEEYDKALSAANKLISSNKK
                     FRASSQDAYFYSALAYEKNGDKEMAKKLYIKALQYENTT"
BASE COUNT          903 a          688 c          673 g         1015 t
ORIGIN      
        1 ggcaggggat taactgaatg taatcaaatc cgttcttctc ataaatattc caaagttttt
       61 gaggatgtct tacgttatat ttattaacaa ccgtaagtat gttgaatgta acttcgtatt
      121 tttttaacag attaataccg ttcattactc tgttgaaggt gtctttttga aaattgtcct
      181 ttctgagatt gtcatgtgct tcttttaatc cgtcaagcga tacgcccgcc agaaagtcat
      241 gttctttgaa taacctgcac catgtctcgt ctataagtaa gccgtttgtc tgtattgaat
      301 acatgtactt tattttttca gtctttgcct gttgtgcgta ttccgtgaaa tcaagaaaga
      361 aatctaattt tgcaagagtg ggttctccgc cctgaaagga aaaggttatt tctccctcgg
      421 gacaagcctg cagtgcccgg tctattattt tatgtgaagt atcttgagac attatcccgt
      481 aggactttat ctttcttttg ttgcttacat cgtcataaaa acaatattta caattcatat
      541 tgcaaagaga agaagccggc tttataagta catggaaatt ttttctcatt tcattgtcct
      601 tcaacttctc ggattttatc accatgtatg ctttttggtc aaacggatat atttcgtgat
      661 attaaaaaaa cgcctcttac ctgtgggaca aaaagacgtt tctcatatct gtggagctgg
      721 tggacggact tgaacccccg acctgctgat tacaaatcag ctgctctacc agctgagcta
      781 caccagcatg atggtgggag caatagggct cgaacctatg accctctgct tgtaaggcag
      841 atgctctccc agctgagcta tgctcccacg gtgttacagc attttgaagt atactaaaag
      901 cacaaacggt tgtcaacagg gattttaaaa acacgacaac cggacaaaca gcggttttta
      961 tgttgtgtat aacttgtttc cttttttttc ggaatactta attgccagtc cgccttcgga
     1021 agtttctctg tatttactga agagatccct tcccgtttca tacatggtat ccacaaccat
     1081 atcgaaggat attttccttg tatgcgtaag aaagttggca aggcttaccg agttgattgc
     1141 tctcattgcg gcaacggcat ttctttcaat gcaaggaatc tgtacaagac ccattatagg
     1201 gtcgcaggta agtcccagat ggtgctccat tgcgacttcc gccgcatatt caatctgatc
     1261 cagatccatg ccgaagagtt cggcaagtcc cgccgatgcc atggaacatg ccgttcctat
     1321 ttccgcctgg catccgcatt cggcaccgga tatggaagca ttggttttta ttacatttcc
     1381 tataactccc gccgcagcaa gagctctcaa aatatccctg ttggaatagc cgtttttttc
     1441 ctgcatgtat ttcagtaccg caggcaacac cccgcaggat ccgcaggtag gcgccgttac
     1501 tacaatacct ccgccggcgt tttgttccga tgccgcaaaa gcataagagc agacaatgcg
     1561 gttttctctg gtggaaggac tttcatccat gtgcctctgt ctgtacaggt accgggcttt
     1621 ccgctgaagt tttaatccgc catgcagtat tcccgtggca ctgagtccct ctttaatgga
     1681 caatttcata tgatgccaaa catcttccag ataattgtat atttccggtc cttcgcattg
     1741 ctcgacatat tcataaagct ttaagtggtg tttttcgcaa tattttttaa tctctgtaaa
     1801 attcttttct ttatagaaat cgggagagtc catgtatggt ttgccctcga ttcttatggc
     1861 tccgccgcct atgctcatca ctctccactt gtccgctacc tcgttatttt taatagccac
     1921 aaaatccatg gtgttgggat gaggcagatc ttcggtgacg gtatctatat gtactttaac
     1981 cggtttatcg ggcaaagcct cttttaccgc cttgtcggtc atatgaccca atcctgtttt
     2041 tgccagcgag ccgtacaaag tcacctcaaa tctttcggca tcgggatgtt cctctccgaa
     2101 ccttcttgcc gccctgtccg gtcccattgt gtgagagctt gacggacctc ttcctattct
     2161 gtacagttct tttaaagatt tcattattat taccttcgct tagaggcaat tatatcatag
     2221 atataattta ataccagtat agtgatgcct tatatatatc ggagggtatc agtaacggga
     2281 aagcactgta aaatagtttt aaaaaagaaa aaatccgagg ggagcggatc ttttcttgct
     2341 aagatttgat tgctgacatc agagtcaggg tacgagcgta ttatacaata tacgaacata
     2401 tgtttcaata gcaaaatttt tactgtccga ttaattaccc agtggtttct ctgttgtaaa
     2461 gagagcggcc gatgatatat aatttagggg caggaaagag ggatttgtga agaataaatc
     2521 tgtaataaaa accattaaca taattgttcc gatttttatt ctcggtctta ttatctttaa
     2581 ctcgacaata gaagcgctgg tgcttgccgt tttttacttt atatatagga taataatcgg
     2641 attatccaag atatattcat atatagccat aaagtattat gtaaaaggta atttaaagaa
     2701 ggcaagaaag tactataaaa aggcatatga agtaaaagta aaggaaccgc ccattgtcgc
     2761 atcatacgca tacctcctta tattgctcgg cgagtatgat caggccgaac aggtacttaa
     2821 ggcactggaa agcatgaagc cggtaggcaa agccgccaca tcgctgatgc tttgtaatgc
     2881 ggtgcttatg tggaaaaggg ataaaaacct tatcgcggca cttgctcatg tggaatcgat
     2941 ggatgagacc atgcgcaatc aatggtatta caatgtatat gccaaactgt gcattttttc
     3001 cggtaatatt ggaaaggcaa aggaagccgc cctgaagggt tatgaatatc ttgagagcaa
     3061 tccggtagcg gtggaaaatc ttctcattgt gcactgtata aatgaagagt atgacaaagc
     3121 tctttccgct gcgaacaaac ttataagcag caacaaaaaa ttccgggcat catcacagga
     3181 tgcatatttc tacagtgcgc tggcttatga aaagaacggc gataaggaga tggcaaagaa
     3241 attgtatata aaagctttac agtatgagaa cacgacaat
//
LOCUS       DAOPWC010000145         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30028_length_3267_cov_2.626401, whole genome shotgun sequence.
ACCESSION   DAOPWC010000145 DAOPWC010000000
VERSION     DAOPWC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30028_length_3267_cov_2.626401"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKJ65_04050"
     CDS             <1..550
                     /locus_tag="PKJ65_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41039.1"
                     /translation="KILISGFQPFANFQDNITRKLVEGLPDIINGNKIYKIILDVTYK
                     TAFDVLDKEINKVRPDIVIATGLAAARKVIATERIALNINHANGADNAGVTKTDSVIV
                     PGAETALTATLEYPECVYKTDIVGKSYSAGTYVCNDLFYRLLHKGTVQAYPYISFIHF
                     PSEENIPYEKQLNTFIDILSCL"
     gene            complement(607..1509)
                     /locus_tag="PKJ65_04055"
     CDS             complement(607..1509)
                     /locus_tag="PKJ65_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41040.1"
                     /translation="MVRKDFKDLKLYFSNSMISLKEGDYEHAIKSFSHLIDQGIEPQK
                     SVIGLITAYSCLTRYPAALKLYEKNKDIFIGNPSNRNMLVETMTALLMKETSLLKKNA
                     RGSLSTVFMAKRMKAVHEAYLTDKDNLLAIILICYWYAVLGARPYETEQMMKDFLRNE
                     YVYDEFRWKLLEKLAITDKELMDDITIAGMFRRIPRYLDHSYINLLLFSHLLGDDFAS
                     AREKIEVQRMNGVELSDDVMWNYINSSVENNDIDDLSVNFAKRLFAKGWMDPVIGQVF
                     RYAKNNLNIYNVTNETKALDLFGI"
     gene            complement(1518..2555)
                     /locus_tag="PKJ65_04060"
     CDS             complement(1518..2555)
                     /locus_tag="PKJ65_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ41041.1"
                     /translation="MKQRVSYLKKVEPENYWPSFADVMSTMVLIMLFLVLIVFIKNII
                     ISINLDEQEATNIATGAQLEATRNDLAAKELALQETQLDLETKQTILIMLEEELAAKE
                     ISLSLLENELAAKQAALELSEEEATNLQNLILILAEQQAALETQKKELLELIALNTIE
                     LDSMREQLQKVAVMQVEIFEKVKKSIDEALGTGYVTIGDDGNLQINASLLFKSDSYEI
                     AATAYPLLNKLAIAFEDILKDSGKYIESIMIEGHASTDGTYEKNQVLSTNRANAVLLY
                     LFDSNKKLETTYANYFGAVGYSESRPIIKNDYTEALRAKNRRIEFSIVVREENVGQII
                     QEYLDSIDKKK"
     gene            complement(2585..>3267)
                     /locus_tag="PKJ65_04065"
     CDS             complement(2585..>3267)
                     /locus_tag="PKJ65_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41042.1"
                     /translation="SQALDTTVERFDSAISTLAVAMRDMSDFNLNLKENVEKMDVSFI
                     KMSESLSDSTNLIIKNYDVIRMFADDVKNAAGQMAISNQETLEQLGYLANQVDQTVSA
                     LQQLTHSMKQSSEENAASFNNMRDAFEKAIIATSMEISSLTDKIKQSFQEALKESSDI
                     IAEKTADTMEKSMANIYQMSESFENNQKILAQTIASLPEQTMAYNKSVSGKIQKKLDE
                     IEKAIKND"
BASE COUNT          889 a          680 c          619 g         1079 t
ORIGIN      
        1 gaagatacta atctcggggt ttcaaccatt tgcaaatttt caggataata ttacgaggaa
       61 actggtagaa gggcttcccg atattatcaa tggaaataag atatataaaa tcatactgga
      121 tgtaacatat aagacggcgt ttgatgtact tgacaaagag ataaataaag tgcgtcccga
      181 cattgtcatt gctaccggat tagccgcagc aaggaaagtt atagccacgg aaagaatagc
      241 gttgaatata aatcatgcaa atggcgcgga caatgcggga gtaacaaaaa ccgacagtgt
      301 aattgttccc ggggcggaaa cagcattaac cgcaacactg gaatatccgg aatgtgtata
      361 taaaacggat attgtcggga aatcctattc ggccggcaca tatgtatgca atgatttatt
      421 ttacaggtta ctgcataaag gtaccgtaca ggcataccca tatatctctt ttattcattt
      481 tccctcggaa gaaaacatac cctatgaaaa acagctcaat acatttattg atatactgag
      541 ctgtctgtaa tactattcat cgcttaataa gtgtttagcg gtaaggtata aagtttaata
      601 aaacaatcat ataccgaaaa ggtccaatgc cttggtctcg ttggttacgt tataaatatt
      661 caggttgttt ttggcatacc gaaaaacctg ccctataacg ggatccatcc agccctttgc
      721 aaaaagccgt tttgcgaagt tgaccgataa atcatcaata tcgttgtttt ccacactgga
      781 attgatgtaa ttccacatta catcatcgga aagttccact ccgttcatcc tctgaacttc
      841 aatcttctcc cttgcagaag caaaatcatc acctaagagg tgggaaaaga gaagcagatt
      901 tatatatgaa tgatccagat atctcggtat tctcctgaac atacccgcta tagttatatc
      961 atccataagt tccttgtcgg taatggccag tttctccaaa agcttccagc ggaattcgtc
     1021 atatacatat tcattacgca gaaaatcctt catcatctgt tccgtttcat aaggtcttgc
     1081 tcccaataca gcataccagt aacatatgag aataatggcc agaagattat ctttatccgt
     1141 aaggtatgcc tcatgaacgg ctttcatacg tttagccatg aatacggtgg aaagcgatcc
     1201 gcgtgcattt ttcttcaata aacttgtttc cttcataaga agagcggtca tggtttccac
     1261 aagcatgttt ctgtttgacg ggttgcctat aaaaatatcc ttattctttt catacagctt
     1321 taatgccgcc ggatacctgg taagacagga ataggcggta attaatccta tgacggattt
     1381 ctgaggctcg ataccttggt caataaggtg ggagaaactt tttatggcat gctcatagtc
     1441 tccttctttg aggcttatca tggaattgga aaaatacagc tttaaatctt taaaatcttt
     1501 tcttaccatt gtctctccta ttttttctta tctatcgagt ccagatactc ctgaattatc
     1561 tgtccgacat tctcttcccg tactactatg gaaaattcta ttcttctgtt tttggcacgt
     1621 aatgcttcgg tataatcatt ttttattata ggacgtgatt cggaataacc gaccgcaccg
     1681 aaataattag cataggttgt ttccagtttt ttattgctgt cgaataaata cagcagaacg
     1741 gcattagctc tgtttgtgga caaaacctga ttcttctcat aagtgccgtc ggtactggca
     1801 tgaccttcaa tcataatgga ctcaatgtat ttaccggaat ctttcagtat atcttcaaaa
     1861 gcgatggcca atttatttaa taacggatat gcggtagcag ctatctcgta gctgtcactt
     1921 ttgaatagca gactggcatt tatctgcaaa ttaccgtcat caccgattgt gacatatccg
     1981 gttcctaatg cttcgtctat ggatttctta accttctcga atatttccac ctgcattacg
     2041 gccactttct gaagttgttc tctcatggaa tccaactcga ttgtatttaa agctatcagt
     2101 tccagaagtt ccttcttctg agtttccagt gcagcctgtt gctctgccag aatcagtatg
     2161 aggttttgca ggttagttgc ttcttcctcc gataactcca atgcggcctg ttttgccgcc
     2221 aactcgtttt ccaacagact caaggatatt tcttttgctg caagttcttc ttccagcatg
     2281 ataagaatgg tctgttttgt ttccaaatcc agttgtgttt cctgaagagc aagttccttg
     2341 gctgcaagat cgtttctcgt tgcttccagc tgagctccgg tggcaatatt agtggcttcc
     2401 tgttcatcca gatttatgga tattattatg tttttaatga acactatgag aactaagaag
     2461 agcattatca gcaccattgt ggacataacg tccgcaaatg acggccaata gttctccggt
     2521 tctactttct tcaaatagct cactctttgt ttcaattaga tcacctctta tctcttatct
     2581 tacgttaatc attttttatg gctttttcaa tttcatccag tttcttctgt atctttcccg
     2641 aaaccgattt attgtatgcc attgtctgtt ccggtaaaga tgctatggtc tgtgccagaa
     2701 tcttctgatt gttctcaaag gattcggaca tctgatatat attagccatg gatttttcca
     2761 ttgtatcggc agtcttttcc gctataatat cgctgctttc tttaagagct tcctggaagg
     2821 attgcttaat cttatcggtc agggaagata tttccatgga ggttgcgatt atggcctttt
     2881 caaatgcatc tctcatatta ttgaatgatg cggcattttc ctcactggat tgcttcattg
     2941 aatgtgtaag ttgttgcaaa gcggaaacgg tctgatctac ctgatttgcc aaatagccga
     3001 gctgttccag agtttcctga ttggaaatag ccatctgtcc tgcggcattc ttaacatcat
     3061 cggcaaacat ccttataaca tcgtagttct tgattattag gttggtggaa tcggataacg
     3121 attcgctcat ctttatgaaa gatacatcca tcttttctac gttttcttta aggttaaggt
     3181 tgaagtccga catatctctc atggcaacag ccaatgtgga tattgcgctg tcgaatcttt
     3241 ccacggtagt gtcaagtgcc tgagatg
//
LOCUS       DAOPWC010000146         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30360_length_3241_cov_3.377589, whole genome shotgun sequence.
ACCESSION   DAOPWC010000146 DAOPWC010000000
VERSION     DAOPWC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30360_length_3241_cov_3.377589"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="PKJ65_04070"
     CDS             <1..1758
                     /locus_tag="PKJ65_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ41043.1"
                     /translation="ISATCWATIQTGASPHMHGITDENIVPEGAYLNEFVSAYHGDYL
                     MAERFWEAAARIGKKSLIADFCVSGPARTDLARTVGGIGSVADSRVLATEQTLRTVKE
                     ISEQLYVFDADVSSIFPSLPAFAMTASGKWQPQIKLQQSKYDKTDKGYFRLHVEKKND
                     LENPFNIEEFYWYMDVTKKGINIAVSEKDMQAGKYYEVPMNGWSEVIKRTLKDDNGPG
                     NYRFRIKLLPAGKNGGFMVFITSAVNMYNALSPESFAKRADNIDEICSSNSYAHFLTR
                     EGNIDTAFELIEFKNEWWKKIILDAMENDPADIMAFVMGHPDSINHMYRSIFEGLQNP
                     TEEQSAVAKYAYKRAYEILDDFIGWLYEHVLGEDTVFMIIGDHGSIGFNRTVLVHDYL
                     EKEGLLNYLPEPDGKRLYWRNRRIDWKNTKAFPVGCANVYVNLKGRQPEGIVEPEDYD
                     KTVNEIIKALYKHHWNEKGQCYIAFAVEKQQAGFVGLGTERVGDVLYGLSGNTTGGYI
                     GGVHACQIPSAKTATGDIRCLFALSGKNVKKGQIIDRPANIEDVAPTLCYLLKYPQPA
                     DATGGVLFHLFEDDMYQKS"
     gene            complement(1746..2462)
                     /locus_tag="PKJ65_04075"
     CDS             complement(1746..2462)
                     /locus_tag="PKJ65_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNZ41044.1"
                     /translation="MLVTVIGRGHSGTRAMSQTLSESGVYMGEPLNTSWDLLPPQDMY
                     EACRVFGKYVRYTGNMKWDFSRVMAMDPDPVFVKLIESYLKSVLTSDRDKKGWKIPET
                     TLVYPWIRKMFPKAYYIHWVRDPRDCILKKHMTDDLNDFGIEYDKTEDVRRNRAISWY
                     YQRELVRSTPVAQREIHIRFEDMIFKQDETLKRLEQFLHIPMAKIPMRTDSVGRYKTD
                     EEVHMFDFFKKDMEECGYDF"
     gene            complement(2559..>3241)
                     /locus_tag="PKJ65_04080"
     CDS             complement(2559..>3241)
                     /locus_tag="PKJ65_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ41045.1"
                     /translation="GLTDLMDNPNTIDPFINGILNTILMKDVIQRNDVRDPSLLENIL
                     RFLAKSIGSPVSVKRITDYIISSGRKTTFDTVDSYVTMLEKSYLLYRVKRYDIKGLAL
                     LKTNDKLYLTDLGLRRILLRRQTEDYGALLENIVFFELLRRGYYVEVGKYDDLEVDFV
                     ATKNGERLYIQVTASLLNEKTKERELRPLRLIPDYNQRIVLSADRLMPRHIDGIKLIN
                     IIDFLLAR"
BASE COUNT          959 a          647 c          720 g          915 t
ORIGIN      
        1 atatccgcta cctgctgggc taccatacag accggagcaa gccctcatat gcacggaata
       61 accgatgaaa atatcgtacc cgaaggggca tatctcaatg aatttgtttc cgcttatcac
      121 ggtgattacc ttatggcaga gcgattctgg gaagccgccg caaggatagg caaaaaatcc
      181 ctgatagcgg atttctgcgt atcgggaccg gcaagaaccg atcttgcccg caccgtcggt
      241 ggcataggca gtgtagccga ttccagagtg cttgcaacgg aacagacatt gcgtacggta
      301 aaggagatat ccgaacagct gtatgtattc gatgccgatg tctcatcaat atttccctct
      361 ttgcccgcct ttgcaatgac cgcttcggga aaatggcagc cgcagataaa acttcaacag
      421 agtaagtatg ataaaaccga taaaggatac ttcagactgc atgtggaaaa gaagaatgat
      481 ttggagaatc cgtttaatat agaggagttt tactggtaca tggatgtaac gaaaaaagga
      541 atcaatattg ccgtcagtga aaaagatatg caggcaggta aatattatga agtaccgatg
      601 aacggctggt cggaagttat aaaaaggaca ttgaaagacg ataacggacc gggcaattac
      661 cgattcagga taaaactgct gccggcaggc aagaacggcg gttttatggt atttatcact
      721 tccgcagtta atatgtataa cgcattatct cctgaaagct ttgcaaaaag agcggacaat
      781 attgatgaga tatgctcttc caacagttat gcccattttc ttaccaggga ggggaatata
      841 gatacggcgt ttgaacttat cgagttcaag aacgagtggt ggaagaaaat aattttggat
      901 gctatggaga atgatcctgc ggatataatg gcgtttgtaa tgggacatcc cgacagcata
      961 aaccatatgt acagaagtat ctttgaagga ttacagaatc ccaccgaaga gcaatcggcc
     1021 gttgcaaagt atgcatacaa aagagcatac gaaattttag acgattttat aggatggctg
     1081 tatgagcatg ttttaggtga agataccgta tttatgataa taggcgatca cggctccata
     1141 ggatttaaca gaaccgtact tgtacatgac tatctggaaa aagaaggtct tttgaattat
     1201 ctgcccgaac cggacggaaa gcgtctttac tggcgcaaca ggcgtataga ctggaagaat
     1261 acgaaagctt ttcccgtggg ttgtgccaat gtgtatgtta atttaaaagg tcgccagccg
     1321 gaaggtatag tggagccgga agattatgat aagacggtaa atgagattat taaagcgctg
     1381 tataagcatc actggaatga aaaaggccaa tgctatattg cttttgctgt agaaaaacag
     1441 caggcgggat ttgtaggatt gggaaccgaa agagtgggag atgtgttata cggtctttcg
     1501 ggaaacacta ccggcggata tataggggga gtgcatgcct gtcagatacc ctcggcaaaa
     1561 accgctacgg gagatatccg ttgcttattc gcactatcag gaaagaatgt gaaaaaaggg
     1621 cagattatag acagacctgc caacattgag gacgttgcgc ccactttatg ctatttactg
     1681 aaataccctc agcccgccga cgctacgggc ggtgtgttat tccacctttt tgaagacgac
     1741 atgtatcaga agtcgtaacc gcattcttcc atgtcttttt taaaaaagtc aaacatatga
     1801 acttcctcat cggtcttgta tcgccctacg gaatcggtcc gcatggggat ttttgccatg
     1861 ggaatatgca aaaactgctc cagtcttttc agagtttcat cctgcttaaa aatcatatct
     1921 tcaaatcgga tatgtatctc tctttgtgca acgggggtgc tccttaccag ttctctttga
     1981 taataccagc ttattgcacg gtttcttctt acatcctctg ttttatcata ctctattccg
     2041 aaatcgttaa ggtcatcggt catgtgtttt ttcaatatgc agtctctggg atcgcgtacc
     2101 cagtgtatgt aataagcttt tggaaacatc ttccgtatcc agggataaac caatgttgtt
     2161 tcaggtattt tccagccttt tttatcgcgg tccgatgtaa gaaccgactt aagataactt
     2221 tctataagct ttacgaaaac cgggtcgggg tccattgcca taacgcgtga aaagtcccat
     2281 ttcatgttac ctgtgtatct gacatatttg ccgaaaaccc tgcatgcttc atacatatcc
     2341 tgcgggggaa gaagatccca tgaagtgttt agcggttctc ccatatatac accgctttcg
     2401 cttaaagtct gcgacattgc tcttgtgccc gaatgccctc ttccgataac tgttactaac
     2461 ataaaaaata cctcgcctta aagtgtactt tacaacaaag ataagttata tgcaacatta
     2521 agagagcatg tttaagttaa atagtcaata ttgcatattt accgggctaa caggaagtca
     2581 ataatattga ttagtttaat gccgtcaatg tggcggggca taagccggtc cgcagatagg
     2641 acaattcttt gattgtaatc aggtataagg cgcaaaggtc tcagctcacg ttcctttgtt
     2701 ttttcgttta aaagagaagc ggtaacctga atatatagtc tttcaccgtt tttggttgcc
     2761 acaaaatcca cttcaagatc atcgtatttt ccaacttcaa cataatatcc tcttctcagc
     2821 aattcaaaga atacaatatt ctcaagaagt gcaccgtaat cttcagtttg tctcctaaga
     2881 agtattcttc ttagtccaag atcagtgaga taaagcttat cgtttgtctt cagtaatgca
     2941 agtcccttaa tatcataacg ttttacacgg tataagaggt aggatttctc cagcatggta
     3001 acataactgt ccacggtgtc aaatgttgtt tttctgccgg aggagattat gtaatcggtt
     3061 attctcttaa cggaaacagg ggatccgatt gatttagcta aaaagcgtaa gatattttcc
     3121 aaaagtgaag gatcacgcac gtcatttctc tgaataacat ctttcataag aatggtattc
     3181 aatatgccgt ttatgaacgg atcgattgta ttcggattgt ccatcaaatc tgtaagcccg
     3241 g
//
LOCUS       DAOPWC010000147         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30629_length_3220_cov_2.854028, whole genome shotgun sequence.
ACCESSION   DAOPWC010000147 DAOPWC010000000
VERSION     DAOPWC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30629_length_3220_cov_2.854028"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKJ65_04085"
     CDS             complement(<1..551)
                     /locus_tag="PKJ65_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNZ41046.1"
                     /translation="MTERYIYPEPLIEEIISKNEIVDTVSRYVKIDRKGKDYFGLCPF
                     HQEKTPSFSVVPAKQIYYCFGCNKGGNVINFIKDIENLSYSEAVKLLAERANIVIPEK
                     NSYVDEAVLRKEKRTYEMNSLSARFFYENMRFKISNQAKEYLGKRGIKGETITLFGIG
                     YAPDEWSALTEYLKSKGFNDDEIV"
     gene            660..941
                     /locus_tag="PKJ65_04090"
     CDS             660..941
                     /locus_tag="PKJ65_04090"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HNZ41047.1"
                     /translation="MSTYTLKRSCRLIDERKNKLDVEYFLIEEFSLKEQCKTYGIKVE
                     TRENGKLTDTCSVSDVSVKQDEALYILEKMADYEVTAVTARDVIEDMAL"
     gene            complement(1038..2282)
                     /locus_tag="PKJ65_04095"
     CDS             complement(1038..2282)
                     /locus_tag="PKJ65_04095"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ41048.1"
                     /translation="MIEIKKLANGTRVIFEHMPIGDIISIGIFVKAGSNYENARNNGI
                     SHFIEHMVFKGTESKSAKDIAIMSDRIGGTLNAYTSKDMTCFFIKTTSDNAATALELI
                     SDMLLHPAFEPKTVENEKRVILEEIAMYEDFPEEQVQERLEKEMWKGNPLSLPIAGSR
                     QTVMSFTEDAVRNHMNKFYVSANTVISVSGCFDKKAMFHMISRYFEDYTSGHCEAADK
                     GVLAQFRTADVFKTKKIEQAYLSMAFEGCVHDSPERYPFSVLSGILGQSMSSRLFQTV
                     REEKALAYNIGSYNASFQTGGILNIYAGCNPERYDDMMHCIQEIIEDISRNGPEDEEI
                     TRARDQIRCSMLLGMETTDSRMISNGRNMLLRGRPVSREEAIEKIDKVTKDDVQSIID
                     KYIDWNRKAVSVLRPAKKTKKN"
     gene            complement(2326..>3220)
                     /locus_tag="PKJ65_04100"
     CDS             complement(2326..>3220)
                     /locus_tag="PKJ65_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNZ41049.1"
                     /translation="GALAQKALLGVIPSEEEFPYAIRVVSDVLMSNGSTSQGSVCGST
                     LALMDAGVPIIAPVAGISCGLVTDESDPKNYEMMIDIQGIEDFFGDMDFKVAGTHKGI
                     TAIQVDIKIDGLTPEIIKRAFEITRKGRLYILDEVMLKVIDKPRPELSPYAPKIITMT
                     IDPEKIKDVIGTGGKIINRIIDMTGVKIDINDDGTVYILTVDQQSGDKAVSIINGIVN
                     DLEPGMIFNDAAVTRIMDFGAFVELVPGKEGLVHISQIAHERINKVSDVLSVGDKVNV
                     KVVEIDRQGRVNLSRKALLPR"
BASE COUNT          812 a          757 c          594 g         1057 t
ORIGIN      
        1 actatttcat cgtcgttaaa tccctttgac ttgagatatt cggtcaaagc cgaccattca
       61 tcgggagcat atcctatccc gaataatgta attgtttcgc cctttatgcc tcttttgccc
      121 agatattcct ttgcctgatt gcttatttta aatctcatgt tctcataaaa gaatcgggct
      181 gaaagactgt tcatctcata tgttcttttt tctttccgaa gaaccgcttc atccacataa
      241 ctgttttttt ccggtatcac tatgttggct ctttcagcca acaattttac cgcttcggaa
      301 taacttaagt tttcgatgtc ttttataaaa ttaataacgt ttccgccctt attgcagccg
      361 aaacagtagt atatctgttt tgcgggaact accgagaagg agggagtctt ttcctggtga
      421 aacggacaca gaccgaaata gtccttgcct tttctgtcta ttttcacata tctggaaacg
      481 gtatctacaa tctcgttttt ggaaattatc tcttctatta gcggttcggg ataaatatat
      541 cgttcggtca ttgctcctcc tgcttacatt attcgacgca tattgcttat ttcctttatt
      601 tgtctgttca taaatacata ttttaatact atccttaccg atatacggag gtaaataata
      661 tgtcaaccta cacgcttaaa cgcagctgca ggcttattga cgaaagaaaa aacaaactgg
      721 atgtcgagta tttcctcatt gaagagtttt ctctgaagga acaatgcaaa acttacggca
      781 taaaggtgga aacaagggaa aacggtaaac tcaccgatac atgttcggtc agtgatgtat
      841 cggttaaaca ggatgaggct ttatacatac tggaaaaaat ggccgattac gaagtcaccg
      901 ccgttacggc aagagatgtt attgaggata tggcactata gtactgagct ttttgccaac
      961 acttttcctc ttattggcgc actttcattc ctcttattgg cgcattttcg ctcccattat
     1021 tggcgattgg agcagtatca attctttttt gttttctttg cgggacgtaa gaccgatacc
     1081 gccttacggt tccagtcaat atacttgtct ataatactct gaacatcgtc ttttgttact
     1141 ttgtcaattt tttctattgc ttcttccctg gataccggtc gacccctcag caacatattc
     1201 cgtccgttgg aaatcattct ggaatcggta gtttccattc ctaaaagcat ggaacacctt
     1261 atctggtcgc gggctcttgt tatttcctca tcctccggac cgtttcggga tatatcttca
     1321 attatttcct gaatacaatg catcatatca tcatatcgtt cgggattgca ccccgcatat
     1381 atgttaagta tgccgcccgt ctgaaaagaa gcattgtatg atccgatatt gtatgccaaa
     1441 gctttttctt cccgtactgt ctgaaagagt cttgaagaca tgctctgccc caaaatgccg
     1501 gaaagtacgg aaaaaggata gcgttccggc gaatcatgca cacatccttc aaatgccatg
     1561 gaaaggtatg cctgctctat cttcttggtt ttaaaaacat ccgccgttct gaattgcgcc
     1621 aaaacacctt tatcggctgc ctcacagtga ccgcttgtgt aatcttcgaa gtatcgggat
     1681 atcatatgaa acatggcttt tttatcaaag cagccggata ccgaaataac cgtattggcc
     1741 gaaacgtaaa acttattcat atggtttctt acagcatctt cggtaaaact cattaccgtt
     1801 tgcctggacc cggcaatggg aagggacaga ggattgcctt tccacatttc tttttccaat
     1861 cgttcctgaa cctgttcttc gggaaaatct tcatacatgg cgatttcttc caagataaca
     1921 cgcttttcat tttccacggt tttaggttca aaagcgggat gtaaaagcat atccgatata
     1981 agttccaagg cggtggcggc gttgtccgat gtggttttta tgaaaaaaca tgtcatatcc
     2041 ttacttgtat atgcatttaa tgttccgcct atccggtcgg acatgattgc tatgtctttt
     2101 gcgcttttgc tttccgtgcc tttaaaaacc atatgctcta taaagtgcga aattccgtta
     2161 tttcgggcat tctcgtaatt gctccccgcc ttaacaaata tacctatgct gattatatcg
     2221 cctataggca tgtgttcgaa tattactctt gtaccgtttg ctaatttctt tatttctatc
     2281 attttctctc taaaaagaaa gcccggtgtt acccgggcgt ttgttttatc tgggcaacaa
     2341 agcttttctt gaaaggttta cccttccctg tctgtcaatc tccacgactt tgacatttac
     2401 cttgtctcct acactcaata catcacttac tttgtttata cgctcgtgtg caatctgcga
     2461 tatgtgtacc agtccttctt ttcccggtac cagttccaca aaagctccga agtccataat
     2521 ccttgtaaca gcggcatcat tgaatatcat gcccggttcc agatcgttta ctataccgtt
     2581 tataatggaa acggccttgt ctcccgattg ctggtctacg gtgaggatat ataccgttcc
     2641 gtcatcgttg atgtcaattt tgactccggt catgtcgatt atcctgttga ttatctttcc
     2701 gcccgttcct atgacatcct ttatcttctc gggatctatc gtcatggtta ttatcttggg
     2761 agcatacggg gagagttccg gtctgggctt gtcaataact ttcagcatta cttcatccaa
     2821 tatataaagt ctgcccttac gtgtaatctc aaacgctctt tttataatct ccggtgtaag
     2881 cccgtcaatc tttatatcca cctgtatagc ggtaatacct ttatgcgttc ccgctacctt
     2941 gaagtccata tctccgaaga aatcttcaat accctgaata tctatcatca tttcataatt
     3001 cttaggatcg gattcatcgg tgaccaatcc gcaggaaata cccgctaccg gagcgataat
     3061 gggaacaccg gcatccatta atgccaaagt gcttccgcag accgaaccct gacttgtgga
     3121 accgttggac atcaatacat cggacaccac tcttatagca tacgggaact cttcctccga
     3181 aggaataact cctaaaagag ccttctgcgc aagcgctccg
//
LOCUS       DAOPWC010000148         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30679_length_3216_cov_3.231256, whole genome shotgun sequence.
ACCESSION   DAOPWC010000148 DAOPWC010000000
VERSION     DAOPWC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30679_length_3216_cov_3.231256"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKJ65_04105"
     CDS             <1..623
                     /locus_tag="PKJ65_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41050.1"
                     /translation="NESLAWQLFGSNDIIGKDIFINDVPYNITGVSKDMHGENQAANP
                     HIYMQYDVYQRMDNSAFISCYEVLLPNPISDFALNIVKEYVRLNQMEHEIIQNTERFN
                     LINTFKVLSNLKERNIKTSKVLYPEWENTARITEYKLARLLLLRIIISAMMLTVLIVM
                     IIVYRTNISDFFEKTVKVIKTKAKNTKIAKAIEERRRKEYEKKEYH"
     gene            601..2940
                     /locus_tag="PKJ65_04110"
     CDS             601..2940
                     /locus_tag="PKJ65_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ41051.1"
                     /translation="MKRRNITKILAVILIILLLMTQITACNKRRGVEKTVPEHVYRRT
                     EIALPEKIQYVSNMFLEGERIYVLGVGYNETDYTEYYILYSMNQDGSDPVEKKMDVSF
                     SKIDGYDGSYLSYITALSDQRLLAVVDAWAEDKEKGEYKSDNFFIILDKDGKVQKKIN
                     LNELLKNHITTDYFYAGMLLSDSAGNIYISSDRTIYVLKSDFTFAFKVELTGNSWMQS
                     MFNYSKDRIAVVMSTEGEEGYKMQIRIIDPEKKAFSEEINVNTHLQNNLYQLFTGLDY
                     SLYYSTQSGIYGYDVKSGESRELLNWINSDIENPNLGRMTAVSDKRFVCIAQEYDEST
                     WTSKQSVMILDYIPPEEVVPKYVITLATAFGAYDVRKPVIEFNRNSQEYRIQIKDYYQ
                     DHQDYEDYTEVIDKLNNDIIAGNMPDILLVDINMPFESYVSKGLLYDMYKLLNKDESF
                     NKEDYFTNVLDAMSVNGKLYSISPSFSVSTVAIKSKFVDTNKKGWTMGELQALMAKLP
                     KETETFIGTSRQEMMDMALSITLGRFIDKDSGKCYFDSQDFIDILKFTKGFNEKSFWE
                     DLDYNNLPADFWEKYDRSYEEDRAILNLVYLNSFYQYTQLRDYTFKEDITLIGFPNSD
                     RKGSAIGVENRIAISSKSKLIDGCWEFVKSFLSDKYQESITYQWPVKISAFDKMAEKA
                     MKGEDGYGPIEPLIGDVVYDSIGMPNPMPQEGRKISEDDVKYIKSFLQSVDVLMSYEQ
                     GVMDIINEEAKMYYAGNKTAEETAKIIQSRVQIYVSEGR"
BASE COUNT         1133 a          526 c          683 g          874 t
ORIGIN      
        1 taaacgaaag cctggcgtgg caactgttcg gctctaacga tattataggc aaagacatat
       61 tcattaacga tgttccgtat aacattaccg gtgtttccaa agacatgcac ggggaaaacc
      121 aggctgctaa tccgcacata tacatgcagt atgatgttta tcaaaggatg gacaattcgg
      181 ctttcatttc ctgctatgaa gtattgttgc ctaatcccat atcggatttt gccctgaata
      241 ttgtaaaaga atatgtcagg ttaaatcaga tggagcatga aataattcag aatacggagc
      301 gtttcaatct gataaatacc ttcaaggtat taagcaacct gaaagagagg aatataaaga
      361 ccagtaaggt actttatccc gagtgggaaa atacggcaag gattacggaa tataagttgg
      421 caagattgtt gttgttacgt ataatcatat cggctatgat gctgacggta cttatagtca
      481 tgattatagt ttacagaacg aatatatcgg atttctttga gaaaaccgtt aaggttataa
      541 aaactaaagc aaagaacacc aagattgcaa aggcgattga agaaagaagg agaaaagaat
      601 atgaaaagaa ggaatatcac taaaatactg gcagtcatac ttattatttt actattgatg
      661 acacaaatta ccgcttgtaa taaaagaaga ggtgtggaaa aaaccgtgcc tgagcatgtt
      721 tatcgcagaa cggagattgc attgcctgaa aaaatacagt atgtcagtaa tatgtttctc
      781 gaaggagaaa gaatctatgt attgggcgta ggatacaatg aaaccgatta taccgagtac
      841 tatattctat acagtatgaa ccaggacggt tccgatcccg tagagaaaaa gatggatgta
      901 agtttttcca aaatagacgg ctatgacggg tcgtacttgt catatattac cgcattatcc
      961 gaccaaagac tgttagctgt tgtggatgca tgggcggaag ataaagaaaa gggagagtat
     1021 aaatcggaca atttcttcat aatactggac aaagacggta aagtgcagaa aaaaatcaat
     1081 ttaaatgaat tgcttaaaaa tcatattaca acggactatt tctatgccgg catgcttttg
     1141 tccgattcgg caggaaacat atatatatcg tcagatcgta caatttatgt actgaaatcg
     1201 gatttcacat tcgcttttaa agtggaactt accggtaatt catggatgca aagcatgttc
     1261 aattacagca aagacagaat agctgttgtt atgagtaccg aaggtgaaga gggatataag
     1321 atgcagataa gaataattga tccggaaaag aaagcatttt ccgaagaaat aaatgttaac
     1381 acccatctgc agaacaattt atatcaatta ttcacaggtc ttgactactc tttatactat
     1441 tccacacaat cgggtatata cggatatgat gttaagagcg gcgaaagcag ggaactgctc
     1501 aactggatta actcggatat agaaaacccg aacctcggca ggatgacggc cgtttccgat
     1561 aaaaggtttg tctgtattgc tcaggaatat gatgaatcta catggacaag taagcaatcg
     1621 gttatgattc ttgactatat accgcccgag gaagtggtgc ccaagtatgt tattactctt
     1681 gcaacggcat tcggagcata tgatgtaaga aaaccggtaa tagagtttaa caggaacagt
     1741 caggaataca gaattcagat aaaggattac taccaagatc atcaggacta tgaagactat
     1801 accgaagtta tcgataagct gaacaacgat atcatagcgg gaaatatgcc cgatatatta
     1861 cttgtagata tcaatatgcc gtttgaaagc tatgtctcaa aaggactgct ttacgatatg
     1921 tataaacttt tgaataaaga tgagagtttt aataaggagg actattttac caatgttctg
     1981 gatgccatga gtgtaaacgg taagctctat tccatatcac cctcattttc ggttagtaca
     2041 gttgctataa aaagcaaatt tgtcgacact aataaaaaag gatggacaat gggcgaactg
     2101 caggctctta tggcaaagct gcctaaagag accgaaacct tcataggaac cagtcggcag
     2161 gaaatgatgg atatggcatt aagtattaca ttaggcagat ttatagacaa agattccggt
     2221 aaatgctatt tcgacagtca ggactttatt gatatcttga agtttacaaa gggatttaat
     2281 gaaaaatcct tctgggaaga tctggactac aacaatctgc ctgcggactt ctgggagaag
     2341 tatgacaggt cgtatgaaga ggacagagcc atactaaacc ttgtttacct caactctttt
     2401 tatcagtata cacagttgag agattataca ttcaaagagg acattacatt aatagggttt
     2461 cccaattccg acagaaaagg ttccgccata ggggtagaaa accgcattgc tatttcatca
     2521 aaatccaaac tgatagacgg ttgttgggaa ttcgttaaga gtttcctgtc cgacaaatac
     2581 caggagagta ttacatatca atggccggtt aaaatatcgg catttgataa gatggcggaa
     2641 aaggctatga aaggagaaga cggttacggt ccgattgaac ctttaatagg agatgtggtc
     2701 tacgattcca ttggcatgcc aaacccgatg ccacaggaag gaaggaaaat aagtgaagat
     2761 gatgtaaaat atataaaatc attcctgcag agtgttgatg tcctgatgtc ctatgagcag
     2821 ggtgtcatgg atataataaa cgaagaagcg aaaatgtact atgccggtaa caagacggcg
     2881 gaagaaactg ccaaaataat acaaagcagg gtgcagatat atgtaagtga aggcagatag
     2941 cctaaaaaat gatgaactat attaaaaaaa tacagttagt catattgatt attattgttg
     3001 caggaatttt atcttcctgc aattaagttg ctattcaaga aaccttcagt atactttcat
     3061 ataaaaacga ggagcactat actttattta gacaatcgga ttttacagtt ggaaagtttc
     3121 ttcaaactcc atatggcttt ttctacaaat catttaacga atacctttat tattctaaag
     3181 atgggcatat gaaatacacg aaactatgtg gaaaac
//
LOCUS       DAOPWC010000149         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30767_length_3210_cov_4.084628, whole genome shotgun sequence.
ACCESSION   DAOPWC010000149 DAOPWC010000000
VERSION     DAOPWC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30767_length_3210_cov_4.084628"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKJ65_04115"
     CDS             <1..660
                     /locus_tag="PKJ65_04115"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ41052.1"
                     /translation="KAWKTAILAVFLPVFFFIGARLFNIFVNSAAYEDISLFEPEFVG
                     LSIYGGFSGALLAVALWCLLFRMSVFGILDLCIVPAAISFATARIGCFLNGCCSGKFT
                     SSIFGVVYPKFADAGGLASYPVLPTQLFELTFAVIILVPALVVQKKSDRFPEGSAFLV
                     YANLFCIMRLLILPFRRLPYEPWVKNYFYPAFYLILIFISLVIFLFRYIKNLRVNEKE
                     Y"
     gene            complement(763..1440)
                     /locus_tag="PKJ65_04120"
     CDS             complement(763..1440)
                     /locus_tag="PKJ65_04120"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ41053.1"
                     /translation="MENNNVRRMPLLGDDAPAFTAVTTQGTINFPADYKGKWVILFSH
                     PADFTPVCTTEFMTFGSMIEEFKALNTELVGLSVDSLYSHIAWLRKIQELEWNGKKNI
                     EIKFPLIEDIRMEVSNLYGMVQPGQSNTQAVRAVFVIDPKGKIRTILYYPLSTGRNFD
                     ELKRIIQALQKADADHCATPADWRPGDDVIVPTAGSCGVAKERMESKDKDMYCLDWFL
                     CFRRQKK"
     gene            1595..1906
                     /gene="trxA"
                     /locus_tag="PKJ65_04125"
     CDS             1595..1906
                     /gene="trxA"
                     /locus_tag="PKJ65_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ41054.1"
                     /translation="MAVLNITKNNFEKEVLKSGKTVLIDFWAGWCGPCRMIAPTIEAV
                     AAEVTDKVVGKVNVDEEPELAQRYGIMSIPTLVVIKDGNVVRRAVGVQSKQAILKLLD
                     I"
     gene            complement(2061..3155)
                     /locus_tag="PKJ65_04130"
     CDS             complement(2061..3155)
                     /locus_tag="PKJ65_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNZ41055.1"
                     /translation="MTDNKQNKFWGKVKKYLNHVFIDGLSGMALGLFATLIIGTIIAQ
                     IGSLIGGKAGEYINMVASVAKALTGAGIGFGVAYKFKESILVSVSAGVAGMVGAFAAS
                     ILAGTAFPGTGLLLLGPGEPLGAFLGALAGIEIGHLVSGKTKVDILVTPLVTITAGSA
                     AGLLIGPPISKFMEAVGAVINWGAEQQPFIVGLVVAVVMGCVLTLPISSAALGIILKL
                     SGLAAGAAVAGCCAQMIGFAVSSYRENKVNGLIAQGLGTSMLQMPNIVRKPVIWIPPI
                     VASAIAGPLSAWALKMTCDATGSGMGTSGLVGPIMTFKTMLADGFPVWYTLLSIIGVQ
                     FILPAVISLFVSELMRKKGIIKLGDMKLDM"
BASE COUNT          874 a          702 c          646 g          988 t
ORIGIN      
        1 aaggcatgga aaacggcaat tttggctgtt ttcctgccgg tattcttctt tataggagcc
       61 cggttgttca acatttttgt taattccgca gcttatgaag atatttcgct ttttgagccg
      121 gagtttgtgg ggctatcaat atacggaggt ttttcgggag ccctgcttgc ggtagcgctt
      181 tggtgtcttc tgtttcgtat gtccgtattc ggcattttgg atttatgtat tgttcccgct
      241 gccatctctt ttgcgacagc caggataggg tgctttttaa acggatgttg ctcgggcaaa
      301 ttcacaagca gcatattcgg tgttgtatat cccaagttcg ccgatgcagg cggtttggca
      361 agttatcccg tacttcccac tcagttgttt gagttgacat ttgccgttat tatccttgta
      421 cccgctttag ttgtgcaaaa aaagagcgac aggtttcctg aagggagtgc ttttttagta
      481 tatgcgaatc ttttctgtat aatgagactg ctcattcttc ctttcagaag attgccttat
      541 gaaccttggg ttaagaatta cttttatccc gctttctatt taatactgat atttatatct
      601 cttgttattt ttctgttcag atatataaag aatcttagag ttaatgaaaa agaatattag
      661 ggataaatat agaagaagtg tagaaaaaag caggctttaa cctgctttct tctactgttg
      721 tgttgttatt ctgcatgcat aaggaacatg catgttttca ctctatttct tctgtcttct
      781 gaagcataag aaccagtcaa ggcagtacat atccttgtct ttggactcca ttctctcttt
      841 tgccactccg caggaacctg ctgtgggaac tataacatcg tcacccggtc tccaatccgc
      901 cggagttgcg caatgatcgg catcggcttt ctgtaaagcc tggataatac gcttaagttc
      961 atcaaaattt cttcccgttg ataaaggata atacaatatt gtacgaatct ttcctttagg
     1021 atctataaca aatactgcac ggaccgcctg tgtgttggac tgtcccggtt gaaccatacc
     1081 gtaaaggttg gaaacttcca tacgaatatc ttctataaga gggaatttaa tctcgatgtt
     1141 cttttttccg ttccactcca gttcctgtat tttacgaagc catgcaatat gagaatatag
     1201 tgaatctacc gaaagaccta caagttcggt gttgagagcc ttaaactctt ctatcatgga
     1261 accgaaagtc atgaactccg ttgtgcaaac gggggtaaag tcagccggat gcgaaaagag
     1321 tattacccat ttccctttat aatctgccgg aaagttgatt gttccctgtg tagttaccgc
     1381 tgtaaaagcc ggtgcatcat ctcccaaaag gggcattctt cttacattgt tattttccat
     1441 attttaaatc ctccttgctc cttaattata ctatgtccta attatacaat caatatttac
     1501 taaaaaatgt gataaagtca ctgttgacac ccatgagggt gtatataata tgattaaaat
     1561 gaaagttcgg ttgaaagacc taaggaggta caaaatggca gtattgaata taacaaaaaa
     1621 caatttcgaa aaagaagttt taaaatcagg caagacggta cttatagatt tctgggccgg
     1681 ctggtgcgga ccgtgcagaa tgattgctcc gacaattgag gcggtagcag cggaagtgac
     1741 cgacaaggtt gtaggtaagg tcaatgtaga cgaggaaccc gagcttgcac agcgttacgg
     1801 cataatgagc attcccactt tggttgttat caaggacgga aatgttgtaa gaagagcagt
     1861 cggcgtacag tcgaaacaag ctattttaaa attactggat atataaattt caacccccct
     1921 tttgaaagat atacaaaacc acacattcca aagaataccg ccttcacagg cggtattgtc
     1981 ttttcgtgaa ttattttctt ttattagttt tccttttact ttgtttaatt ttatgtctga
     2041 agagaataaa attgtattgt tcacatatcc agtttcatat caccaagttt gatgatacct
     2101 ttttttctca tcagttccga tacaaaaagc gaaatcacgg caggcagtat aaactgtacg
     2161 ccgataattg aaagtaatgt ataccagacg ggaaatccgt ccgcaagcat ggttttgaaa
     2221 gtcattatgg ggcctaccag tcccgatgtg cccattcccg aaccggttgc atcacatgtc
     2281 attttcaaag cccaggcgga taacggtccc gctatcgcac ttgccactat cggaggaatc
     2341 cagataacgg gttttctgac tatattaggc atttgaagca tggaagtgcc cagtccctgc
     2401 gctattaatc cgttcacttt gttttctctg taactgctta ccgcaaagcc tatcatctga
     2461 gcacagcagc ctgcgacggc cgctcccgct gcaagaccgg aaagttttaa tattattccc
     2521 aaagctgcgg aactgatagg taatgttaaa acacatccca tgactaccgc aacgacaaga
     2581 ccgactatga aaggttgctg ttccgctccc cagtttatta cggcaccgac agcttccata
     2641 aatttggaaa tagggggacc tataagcaga cctgccgctg accctgcggt aattgtgacc
     2701 aaaggtgtaa ccagtatatc gacttttgtc ttacccgata caagatgacc tatctctatt
     2761 cctgccaatg cacccaaaaa cgcacctaaa ggttctccgg gacctaaaag taataaacct
     2821 gttccgggga atgccgtgcc ggccaatatg cttgctgcaa atgctcctac cattcccgca
     2881 acacctgcgg ataccgatac caatatggac tccttgaact tatatgccac accgaatcct
     2941 atcccggctc cggtcagagc tttggctacg gaagccacca tatttatata ttcaccggcc
     3001 ttaccgccta tcaatgagcc tatctgggct attatggtac cgattatcaa agtggcaaac
     3061 agtcccaaag ccattcccga aagtccgtct ataaagacat ggtttaagta tttcttcact
     3121 ttaccccaaa acttgttttg tttattatcg gtcatgcgtt tctcctttat cgtttttcct
     3181 attgaacaac atatattatg ttatgtcaaa
//
LOCUS       DAOPWC010000150         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_30770_length_3210_cov_3.581933, whole genome shotgun sequence.
ACCESSION   DAOPWC010000150 DAOPWC010000000
VERSION     DAOPWC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30770_length_3210_cov_3.581933"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKJ65_04135"
     CDS             complement(<1..481)
                     /locus_tag="PKJ65_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ41056.1"
                     /translation="MNQNTTQDIRERIIDTAMALIKQSDGLTENITVREIAHKADVAV
                     GLINYHFGSKKNLIEICVQRIISKVMETFSGTDNPTTGKDVNGNEFDIADITASVFTF
                     LINNPEISKISMLGDLSQPSTESNSSVSYRAILKTIPINEPENIRKIKAFMLLSTIQS
                     "
     gene            685..1044
                     /locus_tag="PKJ65_04140"
     CDS             685..1044
                     /locus_tag="PKJ65_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNZ41057.1"
                     /translation="MARPMKRRRVCYLPKIKRFGPLDLPTGANGFVNMTVDEYETIRL
                     IDLEGLMQEECAKQMNVARSTVQGIYVEARKKLAESLVNGKVLLIDGGEYLLCDEFVN
                     DCGRRCNRHRRGRCFEE"
     gene            1077..1436
                     /locus_tag="PKJ65_04145"
     CDS             1077..1436
                     /locus_tag="PKJ65_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ41058.1"
                     /translation="MKVAVPVTEKSYESNVYASFGRAPYFLIYDTETKKSKFIENSAA
                     TSMGGAGIKAAQTIIDNKVNVVLAPRCGQNAAHVIQSADILIYKTVAGSAKDNIDAFV
                     AGKLSLLDEIHAGFHRR"
     gene            1447..2313
                     /locus_tag="PKJ65_04150"
     CDS             1447..2313
                     /locus_tag="PKJ65_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41059.1"
                     /translation="MRIAVLSGKGGTGKTLVSVNLAAVAKSATYIDCDVEEPNGHLFF
                     APEGIKEEDVTVKVPKVDETLCNGCRKCVNFCKFNALAHTADNLIIFDDICHFCGGCM
                     LVCPQKALTETDKVIGRIQKGTSEHVSVYSGILNVGVASGVPIINQLLEEKNFAANKL
                     TVIDCPPGASCVVMESIKDADYCILVAEPTLFGVHNLELVYQLVKLFKKPFGAVLNKC
                     LEGENPAERFCIENNIKILGKIPFDTDLGALNSDAKIVAYEEKKYKDMFSSLLDRVVK
                     EVKHATVTDIKR"
     gene            2285..3154
                     /locus_tag="PKJ65_04155"
     CDS             2285..3154
                     /locus_tag="PKJ65_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41060.1"
                     /translation="MRQLLILSGKGGTGKTTIASAFIKLADAKACADCDVDAPNLHLV
                     TKWKTQPQIKDYYGLPKAEINPELCILCGQCKEHCRFDAILLSDGEYKMKPFACEGCG
                     VCELVCPAQAITMIPSVDGELTLYSDNEKVFSTAQLKMGSGNSGLLVTEVKKQMKSVE
                     VNTDFAIIDGSPGIGCPVIASLSGVDMVLIVAEPSISGISDMERIIGTAAIFGIEIAV
                     CINKYDTNIGNTEKIEDYCKKHGIALVGNIPFDPAAVTAINNGQTVVDIDCISGTAVK
                     DVYNRTMALLLAI"
BASE COUNT          983 a          564 c          754 g          909 t
ORIGIN      
        1 cggattgaat ggtagataaa agcatgaatg cctttatttt tcttatattc tccggttcat
       61 taatcgggat tgtcttaagt attgctctgt agcttacgga agagttgctt tccgtgctcg
      121 gttgcgaaag atcacccagc atggatattt ttgatatttc cgggttgttt ataagaaaag
      181 taaaaacact tgcagtaata tctgctatgt cgaactcatt accatttaca tcctttccgg
      241 ttgtcggatt gtctgttccg gaaaatgtct ccatgacctt tgagatgatg cgctgaacac
      301 aaatttcaat caaattcttt ttagaaccga agtgatagtt aataagcccg acggcaacat
      361 ccgctttatg ggctatttcc cgtacggtta tgttttccgt taaaccgtct gactgcttta
      421 ttaaagccat tgcggtatct attattcttt cccttatatc ctgtgtagta ttttgattca
      481 tagcatacct ccatattgaa cactgttcaa taatatatta ctgaacaatg ttcaaattgt
      541 caagttgatt tttacaaacg ataaaaagat aagttgcgga ctcatttgga aaccctgaaa
      601 aatacacaag ttattgacat atgccggaaa caatatataa ttataggtgt agactatttg
      661 tgcaggcaga ggaggtaatc attaatggcc agacctatga agagaagaag ggtttgttac
      721 ttaccgaaga ttaagcgatt cggaccgctt gatttaccta ccggagcaaa cggttttgta
      781 aacatgactg tagatgagta tgagaccata aggcttatag acttggaagg attaatgcag
      841 gaagagtgtg caaagcaaat gaatgtagcc agaagtaccg tacagggtat ttatgttgaa
      901 gccagaaaaa agttagccga atccttagta aacggaaaag ttctgctgat tgatggcggt
      961 gaatacctgc tttgtgatga atttgtaaat gactgcggac gcaggtgtaa cagacacaga
     1021 cgcggcagat gttttgaaga ataggttatt gaatattaaa aaacaggagg attatcatga
     1081 aagtagcagt tccggtaaca gaaaaaagtt atgagtcgaa tgtatatgca tcattcggca
     1141 gagctccgta tttccttatt tacgatacgg aaaccaagaa gagtaaattt atagaaaaca
     1201 gtgccgcaac cagcatggga ggtgcgggaa ttaaggctgc acagacaata atagataaca
     1261 aagtaaatgt tgtattggct ccgcgttgcg gacaaaacgc agctcatgtt atacagtcag
     1321 ccgatatttt gatttataaa acggttgcgg gttcggcaaa ggataacatt gatgcttttg
     1381 ttgcgggcaa actttcgtta ttagatgaaa ttcatgccgg atttcacaga cgctgaggat
     1441 aataatatga gaattgccgt actgagcggt aagggcggta caggaaaaac attggtgtcg
     1501 gtcaacttag cggcggttgc aaagagtgca acatatatag actgtgatgt ggaggaaccg
     1561 aacggccatc tgttttttgc gcccgaagga attaaagaag aagatgtaac ggtaaaggtt
     1621 ccgaaagttg atgaaacatt atgcaacgga tgccgtaaat gcgttaactt ctgcaagttc
     1681 aatgcattgg cgcataccgc tgataatctg attatttttg atgatatctg ccatttttgc
     1741 ggcggttgta tgttggtttg tccccaaaaa gcattaaccg agacagataa agttataggc
     1801 aggattcaga aaggaacttc cgaacacgta tcggtatatt ccgggatatt gaatgtcggt
     1861 gtagcttcgg gagttcccat tataaatcaa ctgctggagg aaaagaattt tgcagcaaat
     1921 aagctgaccg ttattgactg tcctcccgga gcttcctgcg ttgtaatgga aagtatcaaa
     1981 gatgcggatt actgtattct ggtagccgag cctaccttgt tcggtgtaca taacctggaa
     2041 ttggtatatc aattggtcaa attattcaaa aagccgttcg gagcggtgct taataaatgc
     2101 ttagaaggag aaaatccggc agagcggttc tgtatagaga ataatattaa aatcttggga
     2161 aagattccgt ttgataccga tctcggagca ttaaactccg atgcaaagat agttgcttat
     2221 gaagaaaaga agtataaaga tatgttttct tcattgcttg atagggtggt aaaggaggta
     2281 aaacatgcga cagttactga tattaagcgg taaaggcggt accggaaaga ccaccatagc
     2341 aagcgcgttt ataaagttgg cggatgccaa agcatgcgcc gactgcgatg tggatgcccc
     2401 caatttgcat ctggtaacaa aatggaaaac gcaacctcaa ataaaggact attacggatt
     2461 acccaaagcc gagataaatc ccgagctgtg tatcttatgc ggtcagtgta aagagcattg
     2521 tcgttttgat gctatcctgt tgtcggacgg agaatacaag atgaagcctt ttgcttgtga
     2581 aggttgcggt gtatgtgaac tggtttgtcc cgcccaagcc ataacgatga ttccttctgt
     2641 ggacggtgaa ttaacccttt actccgataa tgaaaaagtt ttttccaccg cacagctcaa
     2701 aatgggcagc ggcaactcgg ggttacttgt cacggaagtg aaaaaacaaa tgaagtcggt
     2761 agaagtgaat acggattttg caattattga cggctcaccc ggcataggct gtccggtaat
     2821 tgcttctcta agcggagtgg acatggttct tattgttgcc gaaccttcca tatccggtat
     2881 cagcgatatg gaacgtatta tcggaacggc ggctatattc ggaatagaga tagccgtatg
     2941 tattaataaa tacgatacaa atatcggtaa taccgaaaag attgaagatt attgtaaaaa
     3001 gcacgggata gctcttgtag gcaatatacc ctttgacccg gcagcggtaa cagcaatcaa
     3061 caacggtcag accgttgttg atatagactg catatcggga acagcggtta aggatgttta
     3121 caacaggaca atggctctgc ttcttgctat ttaacggacg gaacatcaat atatatcatt
     3181 gttttttgat cgtttacaac caatgtgtta
//
LOCUS       DAOPWC010000151         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31215_length_3177_cov_3.175208, whole genome shotgun sequence.
ACCESSION   DAOPWC010000151 DAOPWC010000000
VERSION     DAOPWC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31215_length_3177_cov_3.175208"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2687
                     /locus_tag="PKJ65_04160"
     CDS             <1..2687
                     /locus_tag="PKJ65_04160"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNZ41061.1"
                     /translation="AGNPFTKIEDTIPGKLPQIKIIREAANGYSSYGNQIGLTTGLVD
                     EIYHDSYVAKRMEVGALIGAAPLKNVKRCDPADGDVVLLIGGRTGRDGIGGATGSSKG
                     HTEQSLESAGAEVQKGNATIERKIQRLFRNRKMSLLIKKCNDFGAGGVSVAIGELADS
                     LDIYLDNVPKKYEGLDGTELAISESQERMAVVVDKKDVDKALKLAEEENLEATPVAKV
                     TDSGRLRMFWNGHTIVDIERSFLDTNGVKQYAKAYITGKDKNNNPFYLKNRGDKSYKE
                     RLFESVTDLNACSKKGLIEKFDSTIGSATVLMPLGGKYQITPAEVMAAKLPVLNGKTN
                     YCTLMSYGFFPQIFEWSCFTGACYSVLSSVAKIVAAGGDYTNIRLTFQEYFESLRKDP
                     VRWGKPLQAVLGALHAQLELKMPSIGGKDSMSGSFMDMDVVPTLISFAVSHTDAENVL
                     SPEFKKAGNTVIRFSHRQKADSTPDFADSLKIFAAFKELNRKKAILSASTVKHKGAAE
                     ALLKSCFGNMIGFELSADVNSLFTPGYCDIIAEIDSDKIEDIEISYIFDKYKEVDISV
                     VGKTIEDKQFVMGNLSVALSELVEAYTDKYERVFPTKAKTDTPEINNTLYDGKSIHYS
                     KNKIATPYVFIPVFPGTNCEYDTKRAFEDHGAVCDFSVFRNLTPAHIDDSVKDFVKKI
                     NKANIIAIPGGFSSGDEPDGSGKFIATVFRNPYIKEAVYDMLYNRDGLMLGICNGFQA
                     LIKLGLVPYGKMVELKENSPTLTFNSIGRHVSCMVNTKVVSKLSPWFMCADLGEVYTI
                     PVSHGEGRFICTKEEYLNLSKNGQIATQYTDDCNNPSYDIQYNPNGSYYAVEGITSPD
                     GRILGKMGHSERIGQYICKNIPGNKDQMLFASGVKYFR"
     gene            2704..>3177
                     /locus_tag="PKJ65_04165"
     CDS             2704..>3177
                     /locus_tag="PKJ65_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41062.1"
                     /translation="MISKTVIKKVLEFDNPGRIGYDFLDGSFREFKHVELNIPVKEQN
                     KWQKPEFFADMYPQYKDFDGTLRLDEFGNLWGRMAHDTTGGGEVLEGALKSWEDLDSY
                     KLPDIDNEKRFFNAHLSVEPYKERFILGSIPGCTFAIMRKIRKMELFLEDLVLEEE"
BASE COUNT          989 a          567 c          790 g          831 t
ORIGIN      
        1 gagcgggaaa cccttttact aaaatagaag acacaattcc cggaaaactg cctcagatta
       61 aaattattcg tgaggcggca aacggataca gttcctacgg taaccagata ggacttacca
      121 caggcctggt ggatgagatt tatcatgaca gttatgtggc aaaaagaatg gaagtgggag
      181 cgcttatcgg cgcggctcct ctgaagaatg taaaaaggtg cgatccggca gacggtgatg
      241 tggtattgct tataggcgga agaacgggca gagacggtat aggcggtgct accggttcat
      301 ccaaaggaca caccgagcaa tcgttggaaa gtgccggagc ggaagttcag aaaggtaatg
      361 ccacgataga gaggaaaata cagcggctgt tccgtaatcg gaaaatgagt cttttgatta
      421 aaaaatgcaa tgacttcgga gcgggcggag tatcggtggc tataggagag ttggccgaca
      481 gtctggatat atatcttgat aatgtgccta agaaatatga aggtcttgac ggtaccgaac
      541 ttgccatttc cgaatcgcag gagcgtatgg ccgttgtggt ggataaaaaa gatgtggata
      601 aagctttgaa attggccgaa gaggagaatc tggaagctac accggttgca aaagtaaccg
      661 atagcggtcg tttaagaatg ttctggaacg gacacaccat agtggacata gaaagaagct
      721 ttttagatac caacggtgta aaacaatatg ctaaggctta tattacaggg aaagacaaaa
      781 acaacaatcc gttttatctg aaaaaccgtg gagacaaatc atataaagag cggttgtttg
      841 aatcggtaac cgatctgaat gcatgttcta aaaaaggtct gattgaaaaa tttgacagta
      901 cgataggttc cgcaaccgtt ctcatgcctt tgggcggaaa gtatcagata acgccggcgg
      961 aagtcatggc ggcaaagctt cccgtattga acggcaagac aaattattgc acattgatgt
     1021 cctacggatt tttcccgcag atttttgaat ggtcctgctt taccggtgcc tgttattcgg
     1081 tactttcatc ggttgcgaaa attgtggcag ccggaggtga ctataccaat atacgtttga
     1141 cattccagga gtatttcgag agtttaagga aagatcccgt aagatgggga aaacctttac
     1201 aggcggtttt aggagcactg catgctcagt tggagctcaa gatgccttca ataggcggaa
     1261 aagactccat gtccggttct tttatggata tggatgtggt tcccaccctt ataagctttg
     1321 cggtcagtca taccgatgcc gaaaacgtgt tatcaccgga attcaagaag gcgggtaata
     1381 cggttattcg tttcagtcac aggcaaaaag ccgatagcac tcccgatttt gctgattcac
     1441 tgaagatttt tgccgctttc aaggaattga accgaaagaa agcgatattg tccgcaagta
     1501 cggtaaaaca caaaggagcg gctgaagcgt tattaaagag ctgcttcggt aatatgatag
     1561 gttttgaatt atcggccgat gtgaactcct tatttacccc cggctattgt gacatcatag
     1621 cggagattga ctcagataaa atagaggata tagaaatatc atatattttt gataagtata
     1681 aagaagttga tatctcggtt gtaggaaaga caattgaaga taagcaattt gtcatgggta
     1741 acctttccgt tgctcttagt gaattggttg aggcatacac cgataaatat gaaagggttt
     1801 tccccaccaa agcaaaaaca gatactcctg aaataaacaa tactttatat gacggtaaga
     1861 gtattcatta cagtaagaat aaaattgcaa ccccctatgt attcataccg gtatttcccg
     1921 gaaccaattg cgaatatgat acaaaaagag cttttgaaga tcatggtgcg gtatgtgact
     1981 tttccgtgtt cagaaatctt acacctgctc atattgacga ctcggtaaag gattttgtta
     2041 aaaagattaa taaggccaat attatcgcca taccgggcgg cttttcttca ggtgatgagc
     2101 cggacggttc gggcaagttc atagcgacgg tattccgcaa cccgtatata aaagaggcgg
     2161 tttacgatat gctgtacaac agagacggat tgatgctggg catctgtaac ggatttcagg
     2221 cgctcataaa gctgggactg gttccgtatg gcaagatggt tgaacttaaa gaaaacagcc
     2281 ctacattgac ttttaacagt attggaaggc atgtatcctg tatggttaac acaaaagtgg
     2341 tctccaaact gtctccgtgg ttcatgtgtg ccgacctcgg tgaagtatat acaataccgg
     2401 taagtcacgg ggaaggacgg tttatctgta caaaggaaga atatctgaat ctttcaaaga
     2461 acggacagat tgcaacacag tatacggatg attgcaacaa tccttcgtat gatatacagt
     2521 acaatcccaa cggctcctat tatgccgttg agggaataac cagtcccgac ggcaggatat
     2581 tgggtaagat gggacattcc gaaagaatag ggcagtatat ttgcaagaat atacctggca
     2641 acaaagatca gatgctgttt gcatcgggtg tcaagtattt cagataaggt atcggagggt
     2701 aaaatgattt caaaaacagt tattaagaaa gtattggaat tcgacaatcc ggggagaata
     2761 ggatatgact ttttggacgg ttccttcaga gaattcaagc atgtagaact caacattccc
     2821 gtaaaagagc agaataaatg gcagaagccg gaattttttg ccgatatgta tccccaatac
     2881 aaagattttg acggtactct tcgtttggat gaattcggta acttatgggg aaggatggct
     2941 catgatacca cgggcggagg agaagttctg gaaggtgctt tgaaatcctg ggaggatctg
     3001 gattcatata agttgccgga tattgataat gaaaaaagat tttttaatgc acacttaagc
     3061 gtggaaccgt ataaagaacg ctttatactg ggttctatac cgggttgcac ttttgctatt
     3121 atgagaaaaa tcagaaaaat ggaacttttc ctggaagatt tggttttgga agaagaa
//
LOCUS       DAOPWC010000152         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31402_length_3164_cov_2.688003, whole genome shotgun sequence.
ACCESSION   DAOPWC010000152 DAOPWC010000000
VERSION     DAOPWC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31402_length_3164_cov_2.688003"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKJ65_04170"
     CDS             complement(<1..1109)
                     /locus_tag="PKJ65_04170"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ41063.1"
                     /translation="MLKVVKFGGSSLSDATQVKKVFNIVTSEPARKFVVVSAPGKRNA
                     SDEKVTDMLITLAEKALANEDVTKQLNRIIQRYRDISYDLGMNNDIIDTITQDLKNRL
                     NSDKSIKARYLDNLKAAGEDNCAKLVAKYFNLQGEKATYVSPKEAGLLFTAEFGNAQV
                     LPASYQNLKVLHNYEGKVIFPGFFGYSVNGDVVTLPRGGSDVTGAILAVALECDVYEN
                     FTDVDYIYTVNPNIVSDPLPIDHMTFKEMRELSYAGFSVFHEEALTPVYYKFTHVNVR
                     NTNNPTAPGTMIHMEVNQNRKHRITGISGDKGFSTITVRKYLMNREVGFVRKILQMLE
                     EECIPFEHSPSGIDDISIILRSKYLNKEIEDKLICR"
     gene            1366..2256
                     /locus_tag="PKJ65_04175"
     CDS             1366..2256
                     /locus_tag="PKJ65_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNZ41064.1"
                     /translation="MKRKLGDRYDGYRLRNVDTFFLLIPYIMKTRNDSQIFFEDVADV
                     EELDAFVRRNQENIPGLKLYHIFIAALVRLMATRPRVNRFVCGNRIYARKHISISMSI
                     KRNMTDDGEETSIKPMFAPESTLKDVVEIVNNEVNMNKESGSENDTDKFAKLVGHMPT
                     FIIKAVINTLMWMDRIGIMPKAAIDFSPFHCSAYLTNMGSLGIKSVYHHLYNFGTTSV
                     FLAMGKREYQYQASDEGELIKKRMISLKMVVDERICDGYYYASSMRLFSKILQNPDVL
                     LTPPEEVIKDDGIEAPKVKK"
     gene            2262..3026
                     /locus_tag="PKJ65_04180"
     CDS             2262..3026
                     /locus_tag="PKJ65_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41065.1"
                     /translation="MRKRFLKAAAIILWLLVWQILSVIIGKDLLLPSPYITFATLIRL
                     LSQSAFYNQVLYSLLRIVAGFLTAVVSAFLLGVIAGLNKNVEILLSPFITVIRSTPVM
                     SFIILAIIWFSYNLAPLAVNIIMCFPPIYDNVVKGIRSTDNKLLDMAKVYQVRPVKVF
                     TGVYLASVKPFLMAGMNSALGLAFRVAVAAEVLANPQYGIGTKLLQAKYNLDTAEVFA
                     WTFVILLLSFLFEQLFKKITEGGRKNAPAEKSDKII"
BASE COUNT          989 a          559 c          655 g          961 t
ORIGIN      
        1 cgacagataa gtttatcctc aatttctttg tttaagtatt ttgaacgaag tattatggaa
       61 atatcatcta ttcccgatgg ggagtgttca aacggaatgc attcttcctc cagcatctgt
      121 aatattttac gcacaaaacc tacttcacgg ttcataagat attttcttac ggtaatggtg
      181 gaaaatcctt tatcaccgct tatacccgta atacgatgtt ttctgttttg gttaacttcc
      241 atatgtatca tggtaccggg agcggtagga ttattggtat ttctgacgtt aacatgagtg
      301 aatttgtaat aaacaggagt cagagcctct tcatgaaaca cactgaaacc tgcatatgaa
      361 agctcgcgca tctccttgaa agtcatatgg tcaatcggaa gagggtcgga tacaatattg
      421 gggtttaccg tgtaaatgta atcaacatcg gtgaagtttt cataaacatc acactccaga
      481 gcaaccgcca atattgcacc ggtaacatcg gaaccgcctc tgggtaacgt gaccacatcg
      541 ccgttaaccg aatagccgaa aaatcccggg aaaataactt taccttcgta attatgtaaa
      601 accttcagat tctgatagga tgccggaagc acctgggcgt taccgaactc ggcagtaaac
      661 agcagacctg cttctttggg agatacataa gttgcttttt caccctgcag attaaaatat
      721 ttcgctacaa gctttgcgca attatcttct cctgcggctt taaggttatc cagatatctt
      781 gcttttatag atttatctga attaagtctg ttttttaagt cttgtgtaat ggtatctata
      841 atgtcgttat tcataccgag gtcgtaagaa atatcgcggt atctttgaat tatcctgtta
      901 agttgcttag ttacatcctc attcgcaagg gctttttccg ccaaagttat aagcatatcg
      961 gttacttttt catcggaagc atttcttttg cccggagcgg aaacaaccac aaactttctt
     1021 gcaggttcgg aagtaacgat attaaatact ttttttacct gtgtcgcatc agaaaggctg
     1081 gaaccgccga atttaactac tttcaacata tttgtctcct ttattgtccc tgaccttact
     1141 aaaatgaagt agtcaagtca ttcactagat tttaattaca aacaggttaa ttgtcaagaa
     1201 gcattgaaag cagtgtcaat aacctatgat aatttcaccc ctcaagtgtt taatactctg
     1261 taaaatttaa gagtgttttg tttaaatata aattaagaat aaagaatgcc tattcttaaa
     1321 tcataccgat tatgatacaa tagcatggac aatgggggga aacatatgaa aagaaaacta
     1381 ggcgacaggt atgacggata cagattgaga aatgtggata catttttcct gcttatcccg
     1441 tacattatga aaaccagaaa tgacagtcag atattttttg aagatgtggc ggatgtggaa
     1501 gaattggatg catttgtaag aaggaatcag gaaaatattc ccggcttgaa actgtatcac
     1561 atatttatag cagctcttgt caggctcatg gcaacaaggc cgagagtaaa cagatttgtg
     1621 tgcggaaaca gaatatatgc aaggaaacac atatcgatat ccatgtctat aaagagaaac
     1681 atgacggatg acggagaaga aacaagtatt aagcccatgt tcgctccgga atcaacactt
     1741 aaggatgtag tggaaatagt aaacaatgaa gttaatatga ataaagaaag cggatcggaa
     1801 aatgataccg ataaatttgc caaactggta gggcatatgc cgacctttat tattaaagcg
     1861 gtaatcaata cgttgatgtg gatggacaga ataggtataa tgcccaaagc ggctattgat
     1921 ttttcaccgt tccattgcag tgcatattta acaaatatgg gttctttggg tattaaatcg
     1981 gtttatcatc atttatataa cttcggaacg acatcggtat ttcttgctat gggaaaaaga
     2041 gaatatcagt atcaggcttc cgacgagggt gaactgatta agaagagaat gataagtctg
     2101 aaaatggttg tggatgagag aatatgtgac ggatattact atgcatcgtc tatgagattg
     2161 ttttccaaga tacttcaaaa tcccgatgtt ttacttaccc ctcccgaaga agtaataaag
     2221 gacgacggaa tagaagcacc aaaagtaaaa aagtgaggtt aatgagaaaa aggtttttaa
     2281 aagcggcagc aataatattg tggcttttgg tatggcagat tttatctgtg ataataggta
     2341 aagatcttct tctgccttcg ccgtatatta cctttgccac acttataaga ttattatccc
     2401 aatcggcttt ttataaccag gtcctttatt cgctgttaag aatagtggca gggtttttga
     2461 ccgccgttgt atcggcattt ttattaggag taatagccgg tttaaacaaa aatgtggaga
     2521 tattgctttc tccctttatc accgtaatac ggtccacacc ggttatgagt ttcataatac
     2581 ttgcaataat atggttcagt tacaatctgg caccgctggc ggttaatatc ataatgtgct
     2641 ttccgccgat atatgataat gttgtaaaag gtattaggag taccgacaac aaacttctgg
     2701 atatggcaaa agtttaccag gtaagacccg taaaagtttt tacgggggta tatctggcat
     2761 cggtaaaacc ttttcttatg gcgggaatga attccgcttt gggtctggct tttcgggttg
     2821 ccgttgccgc cgaagtgctg gccaatccgc aatacggtat aggaacaaaa cttttacagg
     2881 caaagtataa tctggatacc gcggaagtct ttgcctggac atttgttata ttactgttaa
     2941 gttttctgtt tgaacagtta tttaagaaaa ttacggaagg cggaagaaaa aatgctccgg
     3001 ctgaaaaatc tgacaaaatc atatgatgat aaagtaattt tccgggattt cagtatggaa
     3061 attgaaaaga acaggattac ctgtctttta ggtaaatccg gttgcggaaa aaccacatta
     3121 ctgaatgtct tatcatccat aaccggagaa tttacggtgg agca
//
LOCUS       DAOPWC010000153         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31548_length_3154_cov_3.189093, whole genome shotgun sequence.
ACCESSION   DAOPWC010000153 DAOPWC010000000
VERSION     DAOPWC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31548_length_3154_cov_3.189093"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKJ65_04185"
     CDS             <1..383
                     /locus_tag="PKJ65_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41066.1"
                     /translation="YDTVLGERGTGLSGGERQRVSIARAVLRNPCIMIFDEATAAVDS
                     ETELLIQEAIERLIKGRTTLMIAHRLSTLRKANKILVVDEGRIIESGTPEELLAKKGK
                     YYKLIEIQTMSSKLQKAKEEENVE"
     gene            395..883
                     /locus_tag="PKJ65_04190"
     CDS             395..883
                     /locus_tag="PKJ65_04190"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNZ41067.1"
                     /translation="MKVPYIQGDNAKIERADLTHVNVTMYDGRKFENVQPRRLFPISG
                     LRKYITLLDSEEKEVAIIRNLDTLMEDSKNAVDQCLNQYYLVPKIIRLYEIKEISGNI
                     NMHVLTDKGERKFEIWHRYNDIKIIHGTRMLFRDTADNRYEIEDIDKLDKVSRTKLAT
                     FI"
     gene            907..1233
                     /locus_tag="PKJ65_04195"
     CDS             907..1233
                     /locus_tag="PKJ65_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNZ41068.1"
                     /translation="MKLILAIVNNDDSAIAASALTEAGYFVTKLSTTGGFLMVGNTTL
                     LIGTESKGTEHVIEILSKYCKTRKQTVPSTASFGVGLSNESLPPEVTVGGAAIFVLNV
                     EKNIKI"
     gene            complement(1235..1660)
                     /locus_tag="PKJ65_04200"
     CDS             complement(1235..1660)
                     /locus_tag="PKJ65_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41069.1"
                     /translation="MRKKFNQMTDMEKSVRLKNHYIVHIAFYAFMFNLLPVAFMGSHI
                     EGFLVYMLILGFPAVIYIASIIFGLFNGFKWLYILFPAVLFVPAAFIYYDYSALLYAA
                     LYAAVSALGMFAGFILVRLFTKKPATSAGSEKVQDRKKK"
     gene            complement(1706..2260)
                     /locus_tag="PKJ65_04205"
     CDS             complement(1706..2260)
                     /locus_tag="PKJ65_04205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNZ41070.1"
                     /translation="MVAVIETERLILREFRFDDVKDLYEYASNPNVGPNAGWTPHKNI
                     QESESVLNMFMRADNLWAIVYKENHKVIGSIGLHPDILRSQIKDLKTRCLGYALSYDY
                     WGMGIATEAVKAIQEYAFNVMKLDLLSVDHYPHNSRSMKVIEKCGFKKEGTLRKARLS
                     YRNEPVDIVTYSLLKEEWKELNGR"
     gene            complement(2306..>3154)
                     /locus_tag="PKJ65_04210"
     CDS             complement(2306..>3154)
                     /locus_tag="PKJ65_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNZ41071.1"
                     /translation="QPLIPTDEFVTQGRTPQAYLATGSFISKSDNEKPYPRICAHRGF
                     SALCPENTMLAISAAVALGADEIELDVWPTKDRVMIAMHDPHFSHDKSKKVWEYTFEE
                     VMELDASMGMSPMIAGMKYDTFEDILKKFHHQTIMNLHIKTKFIDKYSNIVFPYDEED
                     FKVIVDLIEKYDCADYVYIAGDEAVMETALKVAPHLKRCCLDGQLDYTLVDKAIKYKC
                     DKLQFFKPYFNDEMIKKAKDNGIICNIFWSDDPKEALGFLDRGIDTILTNNLHLVQQG
                     LKHRLG"
BASE COUNT          990 a          622 c          640 g          902 t
ORIGIN      
        1 catatgatac cgtgctggga gaaagaggta ccggactgtc cggcggtgag cgacagcgtg
       61 tatccattgc cagagcggtc ctcaggaatc cttgcataat gatattcgat gaagctacag
      121 ctgcggtgga ttcggaaacc gaacttctta tacaggaagc cattgaaaga ctgataaagg
      181 gaagaaccac acttatgatt gctcacaggc tgtcgacatt aagaaaagcc aacaagattc
      241 tggttgtgga cgaaggcaga ataattgaaa gcgggacgcc cgaagaactg ctggcaaaga
      301 agggtaaata ctacaaactt attgaaatac agacaatgtc gtccaaactg caaaaagcca
      361 aagaagagga aaacgtggaa taggaggaag catcatgaaa gtaccgtata tacaaggcga
      421 taatgccaaa attgaaagag cggatttgac acatgtgaat gtaaccatgt atgacggacg
      481 gaaatttgag aatgttcagc ccagaaggct ttttcccata agcggtctga gaaagtacat
      541 cactcttctg gattctgaag aaaaggaagt tgcaatcata agaaacctgg atacattaat
      601 ggaagattcc aaaaatgccg tggatcagtg cttgaaccag tattacctgg tacccaagat
      661 aatcaggttg tatgagatta aggagatatc gggcaatatt aacatgcatg tgctgacaga
      721 caaaggggaa agaaagtttg aaatttggca cagatataac gatataaaga ttattcacgg
      781 cacaagaatg ttatttagag acactgcgga caacagatat gagatagaag atattgacaa
      841 gcttgacaaa gtaagcagga caaagcttgc cacatttata tgatataatg cttaacggag
      901 gaccaaatga aacttatttt agctattgta aataatgatg acagcgctat tgcggcatcg
      961 gctcttacgg aggccggcta tttcgtaaca aagctttcca ctaccggagg atttttaatg
     1021 gtaggcaata ccactttgct tatcggtacc gaaagcaagg gaaccgagca tgttatagaa
     1081 atactttcca aatactgcaa aacccgcaag cagaccgttc cgtcaacggc atcgttcgga
     1141 gtgggattat ccaatgaaag ccttcctccc gaagtaacgg taggcggtgc tgcgatattt
     1201 gtattgaatg tagagaaaaa tattaagata tagtctattt cttttttctg tcttgaactt
     1261 tttcggagcc tgcagatgta gcaggctttt ttgtaaataa tctaaccagt ataaaacctg
     1321 caaacatacc caatgcggaa accgcggcat aaagagcggc atatagcaac gccgagtagt
     1381 cgtagtatat aaaagcggcc ggaacaaaca gtacggcggg aaacagtatg tacagccact
     1441 taaatccgtt aaacagtccg aatatgatgg aggctatgta tataactgcg gggaaaccca
     1501 gaataagcat atataccaga aaaccttcaa tatgcgatcc cataaaagcg acaggcagaa
     1561 ggttgaacat aaaagcataa aatgcaatat gaactatata gtggttttta agtcttaccg
     1621 atttttccat atcggtcatc tgattaaatt tctttctcat agattgattt tatagacaac
     1681 acaaatatat tacaagtcta aaaggttatc ttccgttaag ttccttccat tcttctttta
     1741 acaaagagta tgtcacaata tccacaggtt cgtttctata tgaaagacgg gctttacgta
     1801 atgtaccctc ttttttaaaa ccgcattttt ctattacctt catggaacgg ctgttatgag
     1861 gataatgatc gacggataaa aggtccagct tcattacgtt gaatgcatat tcttgtatgg
     1921 ctttaaccgc ttcggtggct atacccatgc cccagtagtc ataagagaga gcatatccca
     1981 aacatcttgt ctttaaatct tttatctgtg agcgaagaat gtcgggatgc agaccgatgg
     2041 agcctattac tttatggttc tctttataga caatcgccca aagattatcg gctctcataa
     2101 acatattcaa aacagattcc gattcctgta tatttttatg cggtgtccat cccgcattag
     2161 gtcctacatt gggattactt gcatattcgt acaagtcttt gacatcgtca aatctgaatt
     2221 cgcgaaggat gagtctttcg gtttcaataa cagcaaccat ggtacctcct tatttatctt
     2281 tccataaagt gttttaatat tattcttacc ctaacctgtg ttttaaacct tgctgtacca
     2341 gatgcagatt atttgtaagt atggtatcta ttcctctgtc taaaaatcct aaagcttctt
     2401 ttgggtcatc cgaccagaat atgttgcata tgataccgtt gtccttggcc tttttaatca
     2461 tttcatcatt gaaatacggt ttgaagaact gcagtttatc gcatttatac ttaatagcct
     2521 tatccacgag agtatagtcc agctgtccgt caagacaaca tcttttcaga tgaggggcta
     2581 ccttcaaagc cgtttccatt accgcttcat ctcctgcaat atatacataa tctgcacagt
     2641 cgtatttttc tatcaaatct actatgactt tgaaatcttc ttcatcgtag ggaaaaacga
     2701 tattggaata tttatcaata aacttggtct taatatgtaa attcataata gtctgatgat
     2761 ggaatttttt gagtatatct tcaaatgtgt catatttcat tcctgcaatc ataggactca
     2821 tacccataga agcatccagc tccattacct cttcaaaggt atattcccag acctttttgg
     2881 atttgtcatg cgaaaaatgc ggatcgtgca tggcaatcat aactctgtct ttggtaggcc
     2941 acacatccag ttcaatctca tctgctccca aagcaactgc cgccgatatg gccagcatgg
     3001 tattttccgg acacagtgcg gaaaaacctc tgtgagcaca tatacgggga taaggttttt
     3061 cattatcact tttagaaata aaactgcctg tcgcaagata agcctgaggt gttcttccct
     3121 gtgttacgaa ttcatcggtc ggtatgagag gctg
//
LOCUS       DAOPWC010000154         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31551_length_3154_cov_2.916747, whole genome shotgun sequence.
ACCESSION   DAOPWC010000154 DAOPWC010000000
VERSION     DAOPWC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31551_length_3154_cov_2.916747"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /gene="eno"
                     /locus_tag="PKJ65_04215"
     CDS             complement(<1..312)
                     /gene="eno"
                     /locus_tag="PKJ65_04215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364084.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ41072.1"
                     /translation="MKQYIEIEGVWAREILDSRGNPTVEVEVFVEGGYVGRAAVPSGA
                     STGAFEAVELRDGDKKVYLGKGVEKAVDNVNEIIAPELEGLNVLDQVYIDRLMMELDG
                     TE"
     gene            complement(324..1076)
                     /gene="tpiA"
                     /locus_tag="PKJ65_04220"
     CDS             complement(324..1076)
                     /gene="tpiA"
                     /locus_tag="PKJ65_04220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ41073.1"
                     /translation="MRKKVIAGNWKMNKTPSETTAFITELSDKVKQTKDIVICAVPFV
                     CIPAALKASKRTKIKIAAQNMHYEDKGAFTGEVSAQMLVDLGVQYVIIGHSERRQYYN
                     ETDATVNKKVIKALKAGLKPIICVGESLEQRETGVTADFVRYQVGYALNGVSAEDMKN
                     IVIAYEPIWAIGTGKTATNEQANEVCRTIRQALNEKYGKKVANATYIQYGGSVNVKNA
                     AELFSMSDIDGGLVGGASLKSDDFCKIVNYKG"
     gene            complement(1073..2263)
                     /gene="pgk"
                     /locus_tag="PKJ65_04225"
     CDS             complement(1073..2263)
                     /gene="pgk"
                     /locus_tag="PKJ65_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNZ41074.1"
                     /translation="MAELKKKTVEDINVSGKKVIARMDFNVPLKDGVITDDKRIREAM
                     KTIKYLSDHGAKTILVSHLGRPKGEFNPKYSLAPIAARISELLGKPVTLAKDVIGEDA
                     KAKAAALKNGDVMLLENVRFHAEEEQNDKEFSKKLASMAEIFVNDAFGTAHRAHSSTA
                     GIADYLPAVCGFLIKKEIEIMGAALAHPARPFVAILGGAKVSDKIGVIESLLDKVDTI
                     LIGGGMAYTFFKAQGHDIGTSICELDKLDFANELIDKAKKKNVNLLLPLDTVVADEFK
                     NDAKKKTVDFNAIPADMMGMDIGEKTVKLFTKEILKAKTVVWNGPMGVFEFENFANGT
                     RKIAKAVADSGSVSIIGGGDSAAAIEILGYADKVTHISTGGGASLEFLEGKVLPGIDV
                     LEDK"
     gene            complement(2315..>3154)
                     /gene="gap"
                     /locus_tag="PKJ65_04230"
     CDS             complement(2315..>3154)
                     /gene="gap"
                     /locus_tag="PKJ65_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNZ41075.1"
                     /translation="IEIGEDFLIVNGKKVMVYASMEPAGIPWKDCGAEYIVESTGVFL
                     TKEKAQGHITAGAKKVVMSAPSKDDTPMFVMGVNQDKYTKDMTFVSNASCTTNCLAPL
                     VKIIHDNFGVLEGLMTTVHATTATQKTVDGPSSKDWRGGRAAAANIIPSSTGAAKAVG
                     KVIPSLNGKLTGMSMRIPTINVSVVDLTVRLAKPTFYEDIKAAIRKASETNLKGIVGY
                     TEDDVVSTDFIGDSRTCIFDAKAGIPLNDTFVKLVAWYDNEWGYSCKIVDLIMHMAKV
                     DAK"
BASE COUNT          790 a          766 c          543 g         1055 t
ORIGIN      
        1 ctccgttccg tccagttcca tcataagtct gtctatgtaa acctgatcca gaacgttcaa
       61 tccctccagt tcgggtgcga ttatctcgtt tacattgtca accgcttttt ctacaccctt
      121 acctaaataa actttcttat cgccgtctct taattccaca gcttcaaaag cacccgtgga
      181 agctccgctg ggaacagccg ctcttcctac atatccgcct tctacaaata cttccacttc
      241 tacagtggga tttcctctgg aatccagaat ctctcttgcc catacgcctt caatctcgat
      301 atattgtttc atatttacct ccgttatcct ttataattaa ctatcttaca aaaatcatcc
      361 gatttaagac tggctccgcc tacaagaccg ccgtcaatat cgctcatact gaaaagctcg
      421 gctgcattct ttacattaac cgaaccgcca tactgaatgt atgttgcatt ggcaactttc
      481 tttccgtatt tttcattgag agcctgtctg attgttctgc agacttcatt tgcctgttcg
      541 tttgtggcgg ttttacccgt gcctattgcc cagataggtt cataagctat aactatgttc
      601 ttcatatctt ccgcagatac accgtttaat gcatatccta cctgatatct gacaaaatcg
      661 gcagttacac ccgtttctct ttgttccagg ctttcaccta cacagattat cggttttaaa
      721 cctgctttaa gagctttaat taccttttta ttaaccgttg catcggtttc attgtagtac
      781 tgtcttcttt cggagtgacc gattattaca tactgtactc ccaaatcgac aagcatttgt
      841 gcagaaactt caccggtaaa agcacctttg tcttcataat gcatattctg agccgctatt
      901 tttatcttag ttctttttga tgcttttaaa gcggcaggta tgcatacaaa aggaactgca
      961 catataacta tatcttttgt ctgctttacc ttatcggaaa gttcggtaat aaatgccgtc
     1021 gtttccgaag gagtcttatt catcttccaa tttcctgcta taactttttt tctcatttgt
     1081 cctccaatac atcaataccc ggcagtactt ttccttccag gaattccaat gatgctccgc
     1141 cgccggtaga aatatgtgtt actttatcgg cataacctaa aatctctatt gccgcagcac
     1201 tgtctcctcc tccgataata gataccgaac cggaatcggc aaccgctttt gctatctttc
     1261 ttgttccgtt ggcaaagttt tcaaactcga aaactcccat aggtccgttc cagacaaccg
     1321 ttttagcctt aagaatttcc ttagtgaata acttaacggt tttttcacct atatccattc
     1381 ccatcatatc agccggtatg gcattgaaat ccactgtctt tttctttgca tcattcttaa
     1441 actcgtcggc tacaaccgta tcaagcggta aaagaagatt aacatttttc ttctttgctt
     1501 tatcaatgag ctcattggca aaatccagtt tgtccaattc gcaaatagat gttccgatat
     1561 catgtccctg tgccttgaag aatgtatatg ccattccgcc gccgataaga atggtatcaa
     1621 ctttatccag aaggctctct ataacaccga tcttatcgga aactttggca ccgcctaaaa
     1681 tagcaacaaa aggtcttgcc ggatgtgcta aagctgcacc cataatctca atctcttttt
     1741 tgataaggaa accgcatacc gcaggaaggt aatctgctat acctgctgtt gatgaatgtg
     1801 ctctgtgagc ggttccgaaa gcgtcattta caaaaatctc cgccatagaa gccagttttt
     1861 tggaaaattc cttatcattc tgctcttcct ctgcatggaa acgtacattt tccagcaaca
     1921 ttacatctcc gttcttaagt gctgccgcct ttgcttttgc atcttcaccg attacatctt
     1981 tagcaagtgt aacgggtttt cctaacaact cggaaatcct tgcggcgata ggtgccaggg
     2041 agtatttggg attgaactct cctttaggac gacccagatg cgataccaga attgttttgg
     2101 caccgtggtc ggaaagatac tttattgttt tcattgcttc acggattcgt ttgtcgtcgg
     2161 ttataacgcc gtctttcagg ggaacattaa aatccattct ggctataacc tttttaccgg
     2221 aaacattaat atcttctact gttttctttt ttaattctgc catgatagtc tcctttataa
     2281 aaaagacccg tattcacggg ccttagtttt tattttattt tgcatcaacc ttggccatgt
     2341 gcatgataag gtctacaatc ttacatgaat aaccccattc gttatcatac caggcaacaa
     2401 gtttaacaaa ggtatcgttt aacggaattc ctgcttttgc atcaaatata caggttctgg
     2461 aatcaccgat gaaatcggtt gaaacaacat catcctccgt atatcctacg atgcctttaa
     2521 gattggtttc cgatgctttc ctgattgccg ctttgatatc ttcataaaaa gtgggctttg
     2581 ccaatcttac cgttaaatcc acaaccgaaa cgtttatggt aggtattctc atggacatac
     2641 ctgtcagctt accgttgagt gaaggtataa cttttcctac cgcttttgcg gcacctgttg
     2701 atgaaggaat gatattggcg gcagccgctc ttcctcctct ccagtctttg gatgaaggtc
     2761 cgtcaacagt cttctgagta gctgttgttg catgaacggt ggtcataagt ccttctaaaa
     2821 ctccgaaatt gtcgtgaata atctttacca gcggtgcaag acagttggtt gtgcacgatg
     2881 cattggaaac aaaggtcata tccttcgtat atttatcctg gtttacaccc ataacgaaca
     2941 taggtgtatc atcctttgaa ggtgcagaca taactacttt ttttgctcct gccgtaatgt
     3001 gtccctgtgc tttttccttt gttaagaaaa cgcctgttga ttctacaata tattcagctc
     3061 cgcaatcttt ccagggtatt cctgccggct ccatggatgc gtaaaccata actttctttc
     3121 cgtttactat gaggaaatct tcgcctattt caat
//
LOCUS       DAOPWC010000155         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31922_length_3128_cov_3.763423, whole genome shotgun sequence.
ACCESSION   DAOPWC010000155 DAOPWC010000000
VERSION     DAOPWC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31922_length_3128_cov_3.763423"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..619)
                     /locus_tag="PKJ65_04235"
     CDS             complement(206..619)
                     /locus_tag="PKJ65_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ41076.1"
                     /translation="MLDLIRNRRNDYRVLDLMNDFFSTDVMTSVQGFKVDISEKDKEY
                     ILEAELPGFDKKDISVELKNNYLTISARRDYEKEEKEKDFIRRERSYGEFSRSFYAKG
                     IKQEDIKAKYKDGVLVVSLPKKEKEEEVSTSIEIK"
     gene            complement(790..1722)
                     /gene="dusB"
                     /locus_tag="PKJ65_04240"
     CDS             complement(790..1722)
                     /gene="dusB"
                     /locus_tag="PKJ65_04240"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNZ41077.1"
                     /translation="MIIQGKEITGLFLAPMAGITDAPFRTICIEHGAALTYTEMISAK
                     GLMYNNEKTKKLLSKTHPSEPLAVQIFGSETEPMKHATEYICSLNPVLIDINMGCPVS
                     KVVKNGEGSALMKDINKAYETAKAVVNASSVPVSVKFRSGYDKQNLNYMEFGKAMQAA
                     GVACLTLHPRTRDMFYSGKADWNHIVQLREAVDIPVIANGDVFSKQDIEDIREYTGCC
                     DVMIARGSLGNPWIFSNGTPTLEQRMQVFRCHMDLQIDYSGEKNAIKEMRKHGAWYFK
                     GLKNSAKLRDLLFKAETKSDIINYIEIILERENA"
     gene            complement(1753..2184)
                     /locus_tag="PKJ65_04245"
     CDS             complement(1753..2184)
                     /locus_tag="PKJ65_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNZ41078.1"
                     /translation="MKIDSIVNGIVLDHIAAGNSLRIYKFLNLDDLECSVAVIRNCRS
                     KKYGKKDVIKIDSIIDMNLDVLGYIDPNITVNIIRDGKIAEKKHLELPERLVNVIKCH
                     NPRCITSIEQEVDHIFKLTDRENIIYRCAYCETKSDKEEIW"
     gene            complement(2193..3113)
                     /gene="pyrB"
                     /locus_tag="PKJ65_04250"
     CDS             complement(2193..3113)
                     /gene="pyrB"
                     /locus_tag="PKJ65_04250"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ41079.1"
                     /translation="MKHLMDINDLTLSQIDQLIATANDIIADKDKYKDVCKGKKLATL
                     FFEPSTRTRLSFEAAMIELGGSILGFSEAQSSSAAKGESVTDTVQTVGCYADIIAMRH
                     PKEGAPLAASKRTTVPIINAGDGGHSHPTQTLADILTIHREKGRLSNMTVGVCGDLKF
                     GRTVHSLINALSRYQGNKYVCISPTELVITDYVKANILEKNKIEYIETTDMEEYMPSL
                     DILYMTRVQRERFFNEADYVRLKDSYILTPDKLKSAKPDLSIMHPLPRVNEISVKVDD
                     DPRACYFKQVVNGKYMRMALILMLLGIEVK"
BASE COUNT          786 a          746 c          505 g         1091 t
ORIGIN      
        1 gagcgccctg ctatcttatc attttaaaca tatgcgtgtc aacacaaatg ttatctattt
       61 ttggacagcc tctaaatatt agcatagcaa tattacacta tcaacaggtt tttttatttt
      121 actcctcagc gtcgttttga ggtaatctta acgtgaaaaa atccggcaag gccggataat
      181 ttcacctatt tgtcaaacct ttaagttact tgatttcaat agaagtagaa acctcttctt
      241 ccttctcttt cttgggaaga cttacaacaa gaacaccgtc cttgtatttc gccttgatgt
      301 cttcctgctt aattcctttt gcatagaagc ttctggaaaa ttcaccgtaa cttctctctc
      361 ttctgataaa atccttttcc ttttcttctt tctcataatc ccttctggca gagattgtaa
      421 gatagttgtt tttcaattct accgatatat ctttcttgtc gaatccggga agttccgctt
      481 caagaatata ttccttgtcc ttctctgaaa tatccacttt aaatccctga acactggtca
      541 tcacatcggt gctgaaaaag tcgttcatca aatccaatac tctgtaatcg tttcttctat
      601 ttctaattaa gtctaacatt cttgtcacct ccatttaact ttgactttct ttgccttata
      661 tacattatat catactttcg atatgtttca agtattcttt ttgactttat ctgaccttta
      721 ggggaaaata aagggaaata tcttacgata tctcgatatc gctcgcccaa tcttgattat
      781 tctcgataat taagcatttt ctctctctaa aattatttct atatagttaa taatgtcgct
      841 ttttgtctct gctttaaaga gcagatctct cagtttcgca ctgtttttaa gacccttgaa
      901 ataccatgcg ccgtgtttac gcatttcttt tattgcgttt ttttcaccgc tgtagtcaat
      961 ctgtaaatcc atatgacacc tgaatacctg catcctttgc tccaatgtgg gagtaccgtt
     1021 tgaaaatatc cacggattgc ccaaacttcc ccttgctatc atcacatcac aacagccggt
     1081 atattccctt atatcctcaa tatcttgctt actgaaaaca tccccgttgg ctataacggg
     1141 aatatcaacc gcttccctta attgcactat atggttccaa tcggctttac cggaatagaa
     1201 catatcccgt gttctgggat gcagtgttaa acatgcaaca ccggcagcct gcatggcttt
     1261 accgaattcc atgtaattaa gattttgctt atcatatccc gagcgaaact tgacactgac
     1321 cggaacactc gatgcattca cgacggcttt tgctgtttca tatgccttgt ttatatcttt
     1381 catcaaagcg ctgccctctc cgttctttac cacctttgat acgggacagc ccatgtttat
     1441 atctatcaat acgggattca acgaacagat atattcggtc gcatgcttca taggttcggt
     1501 ttccgacccg aatatctgca ccgcaagcgg ctcggacgga tgcgtcttac tcaacagttt
     1561 ctttgttttt tcattattat acataagacc tttggcactt atcatttcgg tatatgtaag
     1621 cgctgctcca tgttcaatgc atattgtccg gaaaggcgca tcggttattc ccgccatagg
     1681 ggccaaaaac agtcctgtta tttctttacc ttgtataatc aaaaggtttt atcctttcac
     1741 ttactgctac agttaccaaa tctcttcctt atccgacttg gtttcacaat atgcacatct
     1801 gtatattatg ttctctctgt cggtaagttt aaaaatatgg tctacttcct gttctattga
     1861 ggtaatacat cgaggattat ggcacttgat aacattgacc agtctttcag gaagttccag
     1921 atgtttcttc tcggcaattt tcccgtccct tattatgttg acggtaatat tgggatctat
     1981 atatcctagg acatccagat tcatgtcgat aatggagtct atcttgataa catccttttt
     2041 gccgtacttt ttgcttctgc agtttcgtat aaccgcaacg gagcattcca ggtcgtctaa
     2101 attcagaaac ttatatatcc tcaaactgtt gcccgctgct atgtggtcta aaactattcc
     2161 gtttacaatg ctgtctattt tcatatcatc cctcacttta cctctattcc caaaagcata
     2221 agtataagtg ccatacgcat atacttgccg ttcaccacct gtttaaagta gcatgctctc
     2281 gggtcatcat ctactttaac cgaaatctcg ttcactctcg gcagcggatg cattatgctt
     2341 aaatcgggtt ttgcactctt taatttgtcc ggagtcagaa tatagctgtc ttttaagcgt
     2401 acataatcgg cttcgttaaa gaatctctcc ctctgcactc ttgtcatata caatatatcc
     2461 aacgacggca tatattcttc catatccgtc gtttctatat attcaatttt attcttctcc
     2521 aatatgtttg cttttacata atccgttata accaattctg taggtgatat gcagacatac
     2581 ttattacctt ggtatctgga aagagcattg ataagagaat gtacggttct gccgaacttc
     2641 agatctccgc aaactcccac cgtcatattt gacagtcttc ccttttcccg atgtattgtc
     2701 agaatatccg ccagcgtctg agtgggatgg ctgtgaccgc cgtctcccgc atttattatg
     2761 ggaaccgtgg tcctttttga agccgccaaa ggagctccct ccttagggtg cctcatggca
     2821 attatgtcgg cataacaacc tacggtctgt acggtatcgg taacactttc tccttttgcc
     2881 gccgaagagc tttgtgcctc ggaaaatccc aatatgctac ctcccagttc aatcatagcc
     2941 gcttcaaaac tcagcctggt ccttgtgctg ggttcaaaaa acaatgtcgc cagtttttta
     3001 cccttacaga catccttata cttatcctta tcggctataa tgtcgtttgc cgtagcaata
     3061 agttggtcta tctgtgataa tgtaagatcg tttatatcca tcaaatgctt cataccaaag
     3121 cctccttt
//
LOCUS       DAOPWC010000156         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_31964_length_3126_cov_2.987626, whole genome shotgun sequence.
ACCESSION   DAOPWC010000156 DAOPWC010000000
VERSION     DAOPWC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31964_length_3126_cov_2.987626"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1484
                     /gene="gdhA"
                     /locus_tag="PKJ65_04255"
     CDS             135..1484
                     /gene="gdhA"
                     /locus_tag="PKJ65_04255"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNZ41080.1"
                     /translation="MNITNPYIAKTYEQIISDNPNEPESHQAVGEVFESIAPVIEAND
                     IYEEYAVLERIAEPERIISFKVTWVSDDGKINVNKGYRVQFNSAIGPYKGGLRFHPSV
                     NTSVLKFLAFEQVLKNSLTGLPLGGGKGGADFDPKGKSDMEIMRFCQSFMSELFRHIG
                     ADTDIPAGDIGVGSREVGYLFGQYKRLANQFNGVLTGKGLSYGGSLGRKEATGYGLCY
                     FTEEMLHAMRNDSFEYKDVAVSGSGNVAIHAIEKIYQLKGLPITASDSNGFIYDPNGI
                     NLDVLKQLKEVENRRIADYEKIVKGSIYYPSMKPWAIPCDIALPCATQNELNLEDAKN
                     LVNNNVMAVVEGANMPCTPDAIHYLRNNKVLFGPAKAANAGGVAVSGLEMSQNAARTN
                     WSFEEVDDKLRAIMKGIFNNINNTAKRYCVEGDFVAGANMAGFIKVADAMIAQGIIF"
     gene            1935..2951
                     /gene="pheS"
                     /locus_tag="PKJ65_04260"
     CDS             1935..2951
                     /gene="pheS"
                     /locus_tag="PKJ65_04260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ41081.1"
                     /translation="MLQLDVMLKNAEREILSATDMKKIEELKVQYLGKKGELTQVLRG
                     MSTLAPEDRPAAGKAANDAREKLNQLFSDAEKIISDKAVKERLYEERLDVTVPGRKQD
                     IGHTHPLTQVIRKIENIFIGLGYQIAEGPEVESVHYNFDQLNAPDNHPSRDEQDTFYF
                     DAKRLLRTQTSPVQARVMENNAPPIRIICPGRVYRADEVDATHSPIFHQIEALVVDKD
                     ITMGNLVATLKLFAKEMFGEETEVRLRPHYFPFTEPSAEVDVSCWVCKGKGCPVCKYE
                     GFIEILGAGMVHPNVLRNCGIDPDVYSGFAFGLGVERTAMAVFGINDLRLFYENDARF
                     LSQF"
BASE COUNT          960 a          538 c          808 g          820 t
ORIGIN      
        1 ggtacgttaa ataccctgtt ttttttaata ttgatattga ataatgcttt tacatatgtt
       61 aatatggcta aaatgatttt tgtaattgag gaaattgcaa aattgcaggc attttgaata
      121 ggagcattga aatagtgaat attacaaatc cctatattgc aaaaacatat gaacagatta
      181 tttccgataa tcctaacgag ccggaatccc atcaggcggt aggggaagta ttcgagtcca
      241 ttgctccggt aatagaagca aacgatatat atgaagaata tgccgtgctg gaaagaattg
      301 cagaaccgga aagaattata tctttcaagg ttacatgggt ttccgatgac ggtaagataa
      361 atgtgaataa aggttacagg gtacagttca actcggcaat cggaccgtat aagggagggt
      421 taaggtttca tccgtcggtc aatacttcgg tgctgaaatt cttagccttt gagcaggtgt
      481 taaaaaacag tctgacggga ttgccgttag gaggaggaaa gggcggtgcg gattttgacc
      541 ccaaaggtaa atccgatatg gaaataatga ggttttgtca gtctttcatg tcggaacttt
      601 tccggcatat aggagcggat accgatatac ctgccggtga tataggggtg gggtcgcgtg
      661 aagtcggcta tttattcgga caatacaaac ggcttgccaa tcagtttaac ggtgtgctga
      721 ccggaaaggg gttatcatac ggagggtcgc tgggcagaaa ggaagctacg ggttacggac
      781 tgtgctattt taccgaagaa atgctgcatg ctatgagaaa tgattctttt gagtataagg
      841 atgttgccgt atccggttcg ggaaatgtcg ctatacatgc aattgaaaag atatatcagt
      901 tgaaaggttt acctataacc gccagcgaca gtaacggatt catatacgat cccaacggaa
      961 tcaatctgga tgttcttaag caacttaaag aagtggaaaa cagaaggatt gccgattacg
     1021 aaaaaatagt taaagggtcg atatattatc cttcaatgaa accgtgggca ataccgtgtg
     1081 atatagctct tccttgtgct actcagaacg aattgaatct ggaagatgcg aagaatctgg
     1141 taaacaataa tgttatggct gttgtggaag gagcgaacat gccttgcaca cccgatgcaa
     1201 tacactatct taggaacaat aaggtcctgt tcggtcctgc aaaagccgct aatgcgggag
     1261 gagttgcggt atccggtctg gagatgagcc agaacgccgc aaggacaaac tggtcgtttg
     1321 aggaggttga cgataagctc agagctatca tgaaaggcat attcaacaac attaacaata
     1381 ccgcaaaacg ctattgcgtg gaaggcgatt tcgttgccgg agccaatatg gccgggttta
     1441 taaaagtggc cgatgcgatg attgctcagg ggataatatt ctgataaaaa ctctctgttt
     1501 ttatacatat aatgaatgaa ccgttaaaga tgcccaaggg cgtcttttac gttatatcaa
     1561 tatttcttgt aagttaacgg attttatccg tcttgagtac aaagtttaat tccggtatga
     1621 attggggttt aaaacgaata aaacaaaaat aatattgtat ttcacgcaaa atatgataaa
     1681 atacgataaa tactatgaag aaagaaagta cccttacttg ttttcttaca gagagacggt
     1741 gtcgtggctg agagcaccgt agcgggcggt gcggggaagt tccttttgga gttgttcggc
     1801 cgaacttgca gtaagccgag acggcaggac accgatatag gtcccttgca ttgtcggatg
     1861 tgtagtctca tggtttgaat cagctttcgc cctaccggga tgaaggctca ttttatatta
     1921 cggaggaaag ctaaatgtta cagctggatg tcatgttgaa aaatgccgaa agggaaatac
     1981 tttcggcaac ggatatgaaa aagatagagg aactcaaggt tcagtatctg ggtaaaaaag
     2041 gcgagctgac tcaagttttg agaggtatga gtactcttgc tcccgaagat cgtccggcgg
     2101 cgggaaaagc agccaacgat gcacgcgaga aactgaatca attatttagc gatgcggaaa
     2161 agattatcag cgataaagcg gttaaggaaa ggctttatga agaacgtctg gatgtaaccg
     2221 tgccgggaag aaaacaggat atcgggcata cccatccgtt aacacaggtt ataagaaaga
     2281 ttgagaatat atttataggg ttgggttatc agatagcgga aggtccggaa gtggaatcgg
     2341 tccactataa tttcgatcag ctcaatgcac ccgacaacca tccttccaga gatgagcagg
     2401 atacctttta ctttgatgca aaaaggcttt tgaggacaca gacttcgcct gtacaggcaa
     2461 gagtaatgga gaataacgct cctcccataa gaataatatg tccgggaagg gtatacaggg
     2521 cggacgaggt ggatgcaacg cattcaccga tattccatca gatagaagca ctggttgtgg
     2581 ataaggatat cacgatgggt aatctggttg ccacattaaa actgtttgca aaggaaatgt
     2641 tcggagaaga gacggaagta cggcttcgtc cccactattt cccgtttacc gaacccagtg
     2701 ccgaagtgga tgtcagctgc tgggtatgca aagggaaagg ttgcccggta tgtaaatatg
     2761 aaggatttat agaaatcctg ggagcgggta tggttcatcc aaatgttttg agaaattgcg
     2821 gaatagatcc cgatgtatac agcggatttg cattcggatt gggtgtggaa cgtaccgcaa
     2881 tggcggtatt cggaataaac gatcttcgac tgttttacga aaacgatgca agattcttgt
     2941 ctcagtttta ggaggtagat atgaaagcac ctataagttg gttaaaagat tttgttgata
     3001 tagatgagga tataaagagg ttttgtaatc tgatgaccgt gaccggtaca aaagtggaag
     3061 gttacaatca ggcgggagcg gaaatatccg gtgttttctt ctcctctccg gggaatgcgg
     3121 aaagcg
//
LOCUS       DAOPWC010000157         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32320_length_3102_cov_2.652445, whole genome shotgun sequence.
ACCESSION   DAOPWC010000157 DAOPWC010000000
VERSION     DAOPWC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32320_length_3102_cov_2.652445"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKJ65_04265"
     CDS             <1..838
                     /locus_tag="PKJ65_04265"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNZ41082.1"
                     /translation="FCTYCIVPYVRGKLISFPSAKILDFIHEAVDSGVTEISLLGQNV
                     NQYGLDSADIPFWSLLDRAASIKGLIRVNFLTSHPMDFNDEIIKVIAGNPVISRGIHL
                     PLQSGSDRILELMNRKYSIKRYYEIIDSMHKLIGDFAVSTDLIVGFPGETEADFKETL
                     EVYEKAGFDFAFTFIYSKRTGTPAAKRPDQIPDELKNQRFARLVALVNAMTSASNIRD
                     ENKVLQVLAEGKSRTDRTVMTGRTSSNKIVNFKCDNDFTGHLINVKIKKGHTWYLEGE
                     KI"
     gene            835..1224
                     /locus_tag="PKJ65_04270"
     CDS             835..1224
                     /locus_tag="PKJ65_04270"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNZ41083.1"
                     /translation="MIFDKVDELSELIEDTKQYNDYLEAEVELENDGHGIDMINAFYD
                     LQQEYIKTYNEGSYDEISAMENVLEMRHTELMNYTTTGNYIRAKEALDEFLQQITDRI
                     MSNLGLTAGCGPDCGEDCSECSHGGLN"
     gene            1225..2775
                     /gene="mutS"
                     /locus_tag="PKJ65_04275"
     CDS             1225..2775
                     /gene="mutS"
                     /locus_tag="PKJ65_04275"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ41084.1"
                     /translation="MENLTPMMKQYMKTKEQYKDCILMFRLGDFYEMFFDDAVTASKE
                     LEITLTGRNCGLDERAPMCGVPYHSVDPYIAKLIKKGYKVAICEQLEDPATAKGIVKR
                     DVTRIITPGTVHENTLLDEKENNYIMSLFKRGEVYSLAVCDVSTGELNSCQIIYGNTL
                     EKLINEIVKYKPKEIISNFDTEFIKQYISVYVSDVEQSLFDRSFPDIAIKSKNKTNDF
                     DIWRNACAALYQYLKETGKCDISHISFVNVYEAEQYMSLDLNARTNLELTENLKDNRR
                     YGTLLWVLDKTSTAMGARLIKKWIEQPLNDIDDINLRLDSVSELKDKYIMRQELMELL
                     KSVYDIERLVSKTVMANANGKDLISLKNSLFKLPYIKQLSVKLESDLLRHTDSNLNTF
                     SDIADLIEQSINEECPVILREGNLIKSGFDKGVDELRKASHGGKNWIIEFEEKEKERT
                     GIKKLRVSYNKVFGYYIEVTNSFLDQVPSDYVRKQTLVNSERFITQELKDMEESILGA
                     EEKLIRLE"
     gene            2872..>3102
                     /locus_tag="PKJ65_04280"
     CDS             2872..>3102
                     /locus_tag="PKJ65_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41085.1"
                     /translation="MSEAADRFTYNRPEMTKDCVIDIKEGRHPVVERMPTCPEFVPND
                     LYMNDTTDKTLIITGPNMSGKSTYMRQAALITI"
BASE COUNT         1046 a          563 c          694 g          799 t
ORIGIN      
        1 cttctgcaca tattgtattg ttccgtacgt caggggtaaa ctcatatcat tcccctctgc
       61 aaaaatactt gattttatcc acgaggcagt tgactcagga gtaacagaaa tttcacttct
      121 tggacaaaat gttaaccagt acggccttga ttcagcggac atccctttct ggagtctcct
      181 tgacagggct gcttcaataa aaggattgat ccgtgtaaat tttctgacat cacatccaat
      241 ggactttaat gatgaaataa taaaggtaat agcaggaaat ccggtaattt cacggggaat
      301 tcacctgcct ctgcaaagcg gctcagaccg catacttgaa cttatgaacc gcaagtacag
      361 cataaaacgt tattacgaaa ttattgattc catgcataag ctaatcggcg actttgccgt
      421 ttctaccgat cttattgtgg gatttcccgg agagactgag gccgatttta aggaaactct
      481 ggaagtatat gaaaaggcgg gttttgattt tgcttttacc tttatctatt ccaaacgtac
      541 cggtactcct gccgctaaac ggcccgatca gatacccgat gagcttaaaa accaaaggtt
      601 tgcaaggctt gttgcccttg ttaacgctat gacatcggca agtaatatca gggatgagaa
      661 taaggttctg caggttctgg cggaaggaaa aagcagaacc gaccgaacgg taatgaccgg
      721 caggacaagc agcaacaaaa tagttaattt taagtgtgat aacgatttta caggtcacct
      781 tattaatgtt aaaataaaaa aaggccatac atggtacctg gaaggagaaa agatatgata
      841 tttgataaag tagacgagtt aagcgaatta attgaggata caaaacagta taacgattat
      901 ctggaagccg aagtggagtt ggagaacgac ggtcacggta tagatatgat aaatgcgttc
      961 tatgatctgc agcaggaata tataaagaca tacaatgagg gtagctatga cgagatatcg
     1021 gctatggaga atgtgttgga aatgagacat accgagctta tgaactatac taccacaggc
     1081 aattacataa gagcaaaaga agctctggat gagtttttac agcagataac cgacaggata
     1141 atgagtaatc taggcttgac tgcgggatgc ggacccgatt gcggtgaaga ctgttcggaa
     1201 tgttctcacg gaggcctcaa ctgaatggaa aatcttacac ctatgatgaa acagtatatg
     1261 aaaacaaaag agcagtacaa agactgcata cttatgtttc gtctgggcga tttttatgag
     1321 atgtttttcg atgatgccgt tacggcatca aaagagttgg aaataacgct gaccggaaga
     1381 aattgcggtc tggatgagag agcacccatg tgcggagtgc cttatcacag tgtagaccct
     1441 tatatagcca aacttatcaa aaaaggatat aaagtggcta tatgcgaaca attggaagat
     1501 cccgcaacgg caaaaggcat agttaagagg gatgtcacaa gaataataac tcccggaacg
     1561 gtgcacgaga acacactact tgacgaaaaa gagaacaatt acataatgtc tctttttaaa
     1621 agaggtgagg tgtactcatt ggcggtatgc gatgtttcca cgggggagtt gaacagctgc
     1681 cagattattt acggtaatac gttggaaaaa ctcataaacg agattgttaa atacaaacct
     1741 aaagagataa tatccaattt cgataccgag tttataaagc agtatataag cgtatatgtt
     1801 tccgatgtgg aacagtcatt attcgaccgc tcttttcccg atatagccat aaagagtaag
     1861 aacaagacca acgactttga tatctggaga aatgcctgtg cggctcttta tcaatatctt
     1921 aaggagacgg gaaagtgtga tatttcccat atatcttttg tcaatgtcta tgaagccgaa
     1981 cagtatatgt ctttggactt aaacgccaga accaatctgg aattaaccga aaaccttaaa
     2041 gataacagaa gatacggcac gcttttatgg gttttagata aaacatccac ggcgatgggt
     2101 gccaggctta ttaaaaaatg gattgaacag cctttgaatg acatagatga tattaatcta
     2161 cgtctggatt ccgtttcgga actgaaagat aagtatataa tgcgccagga attaatggag
     2221 cttttaaaga gtgtttacga tatagaaaga ctggtttcca agacggtaat ggcaaatgcc
     2281 aacggcaaag acctcatatc ccttaaaaac tcactgttta aactccctta tataaaacaa
     2341 ctgtcggtaa agctggaaag cgacttattg agacatacgg acagcaatct aaataccttt
     2401 tccgatatag ccgaccttat agaacaaagc atcaacgaag agtgccccgt aatactgcgg
     2461 gaaggcaacc ttataaaatc cggctttgat aaaggggtgg acgaattaag aaaagcctct
     2521 cacggcggta aaaactggat aatagagttt gaagaaaagg aaaaagagag aaccggtatt
     2581 aaaaagttaa gagtttcata taacaaggtt ttcggctact atatcgaagt taccaacagt
     2641 tttttggatc aggtacctag tgactatgtg aggaaacaga ctttggtaaa cagtgagaga
     2701 tttatcacac aggaactgaa agatatggag gaatccatat tgggagcgga agagaagctc
     2761 ataagactgg aataagagat attctgtaaa ataagggata tcattgcttc cgatgccgaa
     2821 aagctcttaa aaacggctta cagcctgtct gtgctcgatg ttttgtgttc tttgagtgaa
     2881 gcagccgaca gatttacata taacagacct gaaatgacaa aagattgtgt tatagatatt
     2941 aaagagggca gacatccggt tgtggaaaga atgcctacct gtcccgagtt cgttccgaat
     3001 gatttataca tgaatgatac cacggacaag accctgatta taaccggacc caatatgtcg
     3061 ggtaaatcca catatatgcg tcaggcagcc ctcataacaa ta
//
LOCUS       DAOPWC010000158         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32366_length_3099_cov_2.839356, whole genome shotgun sequence.
ACCESSION   DAOPWC010000158 DAOPWC010000000
VERSION     DAOPWC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32366_length_3099_cov_2.839356"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..2653)
                     /gene="clpB"
                     /locus_tag="PKJ65_04285"
     CDS             complement(47..2653)
                     /gene="clpB"
                     /locus_tag="PKJ65_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNZ41086.1"
                     /translation="MNIQKFTQKSVEALNRAQSLAIEYGNIQIEQEHLLYVLLSQPES
                     FIAQLIVSMNLDTGLLIQKAENLVSALPKVTGSGREPDKVYISKDTDLTLNEAEKQAK
                     IMKDEYVSVEHIFLAVIETANPRIRELFRETGITKDSFLRALASIRGNTKVTSDNPES
                     TYDVLKKYGQDLVELARNQKLDPVIGRDEEIRNVIRILSRKTKNNPVLIGEPGVGKTA
                     IAEGLALRIVRKDVPDNLKERKIFSLDMGALVAGAKYRGEFEERFKAVLQEVKNSEGR
                     IILFIDELHNIVGAGKTEGAMDAGNLLKPMLARGELHCIGATTLNEYRQYIEKDAALE
                     RRFQPVMVLEPSVEDTISILRGLKERYEVFHGVKIQDQALIAAATLSDRYISDRFLPD
                     KAIDLVDEACALTKTEIDSMPSELDDISRKIMQYEIEEAALKKESDKLSKEQLAKIRQ
                     ELAQLREVFNEMKAKWENEKASISKVQKIREEIEKVNAEIEKAEREYDLNKAAELKYG
                     KLPALTKTLAEQEEIAEQAEQKHTLLRDKVTEEEIALIVARWTGIPVSKLMQSEREKL
                     LGLEDILHKRVIGQDEAVTKVTECILRSRAGIQDPDRPIGSFLFLGPTGVGKTELAKA
                     LAEALFDDEKNMVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYAVVL
                     FDEIEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIIMTSNLGTPYIMDGITEDN
                     EISDEARKMVDTLLKQKFRPEFLNRLDEIVLYKPLSEKEIMSIVDLMIEDLARRVENK
                     RLGLKVSKRAKEYIVDQGFDSIYGARPLKRYIQSHVETLIAKMILAYDLEPDTVLYVD
                     YDGDKLSVDTEG"
     gene            complement(2670..>3099)
                     /locus_tag="PKJ65_04290"
     CDS             complement(2670..>3099)
                     /locus_tag="PKJ65_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41087.1"
                     /translation="DVRTALSYRYRPTIRYCLEILGRDKGPFLKLKAQVRTQSSSLIS
                     AIEDFFTIWQKVEKQLIPDAYDEVEMTSYENVCYTRFFFLNDRMNIEEQRLGKAIAAY
                     ITTLDKALDIFMSNMDNTDYVISDIYAAYKEYYAKTGMII"
BASE COUNT          708 a          799 c          533 g         1059 t
ORIGIN      
        1 acatttatcc tattcaaata agatatctat aaattatttc ctttacttaa ccttcggtat
       61 ctacgctaag tttatcgccg tcgtaatcga catacagtac cgtatccggc tccaaatcat
      121 atgccagtat cattttagct atcagtgtct ccacatgact ttgtatatat cttttcagag
      181 gacgcgctcc gtaaatactg tcaaatccct ggtccacaat atactctttt gcccgctttg
      241 ataccttcag gcccaacctc ttattctcca ctctccttgc cagatcttca atcataagat
      301 ctacaatgga cattatctcc ttttcggata aaggcttgta cagtactatc tcatccagtc
      361 tgttcaaaaa ctccggacgg aacttctgct taagaagcgt atccaccatt tttcttgctt
      421 cgtccgatat ttcgttatcc tccgttatac catccatgat gtatggggta cccaaattgg
      481 aagtcattat tataatggtg ttcttgaagt ccacggtacg tccctgggaa tcggttattc
      541 tgccgtcatc caaaacctgc agcagaatgt tgaacacatc gggatgtgcc ttctctattt
      601 catcaaacag cactaccgca tacggccttc ttcgcaccgc ttcggtaagc tgtccgcctt
      661 cctcatatcc cacatatccc ggaggcgctc ctatcagtct gcttaccgag aacttctcca
      721 tatactcact catatctatt ctgaccatat tcttttcatc gtcgaaaaga gcctctgcaa
      781 gtgcctttgc aagctcggtt ttacccacac cggtaggtcc taaaaacagg aaggaaccta
      841 taggtctgtc gggatcctga atgcccgctc tggaacgcaa gatgcattcg gttacctttg
      901 tgaccgcttc atcctgtcct ataacccttt tgtgcagtat atcttccaat cctaaaagtt
      961 tttctctttc gctttgcata agtttggata caggtatacc ggtccatctt gcaactatca
     1021 gtgctatttc ctcttccgtt accttatctc ttaataaagt atgcttttgt tccgcctgtt
     1081 ccgctatctc ttcctgttcg gcaagggttt ttgtaagggc aggcagtttt ccgtacttaa
     1141 gctcggcagc tttattgagg tcatattctc tttcggcttt ttcaatctcg gcattgacct
     1201 tctctatctc ctcacgtatc ttctgcactt tggatatgga agccttttca ttctcccact
     1261 tggctttcat ctcattgaac acttctctca attgggcaag ctcttgcctt atcttggcca
     1321 gctgctcttt ggaaagtttg tcgctttctt ttttcagtgc cgcttcctca atctcatact
     1381 gcattatttt cctagatata tcatccagct ccgaaggcat ggaatctatc tcggtttttg
     1441 taagggcaca tgcctcatcc accaaatcaa tggctttatc gggcaggaat ctgtcggata
     1501 tatatctgtc ggacagtgtt gccgccgcta ttaatgcctg atcctgtatt tttactccgt
     1561 ggaaaacttc atatctttcc ttaagtcctc tcagtatgga aatagtatct tctacagatg
     1621 gctccagtac cattaccggc tggaatcgtc tttccagagc ggcatctttt tctatatact
     1681 gacgatactc gttcaatgtc gtcgcaccta tacaatggag ttctcctctg gcaagcatgg
     1741 gtttgagcag attgcccgca tccatggctc cctcggtttt acccgctccc actatattgt
     1801 gtaattcatc aataaaaagt attattcttc cttcggaatt ctttacttcc tgaagtaccg
     1861 ctttgaatcg ctcttcaaat tcccctctgt attttgctcc tgctacaagt gcacccatat
     1921 ccagggagaa tatttttctc tccttcagat tgtccggtac gtcctttcgt actattctca
     1981 atgccagtcc ttcggcaata gcggtctttc ccacacccgg ctcacctatc agcaccggat
     2041 tattcttggt ctttctggac agtatcctta taacattcct tatctcttcg tcacgcccta
     2101 ttaccgggtc cagtttctga ttccttgcaa gctccaccag atcctgaccg tattttttca
     2161 gcacgtcata tgtagactcg ggattatccg atgtcacctt ggtgtttcct cttatggaag
     2221 ccagtgctct taaaaaggaa tctttggtta tgcctgtttc tctgaacagc tctcttatcc
     2281 tcggattggc cgtttcaata acggcaagga atatgtgctc taccgacaca tactcgtctt
     2341 tcattatctt tgcctgcttt tccgcttcat ttagtgtcaa atcggtatct tttgaaatat
     2401 ataccttatc cggttccctg ccgcttcccg ttaccttggg aagtgcggat accaggttct
     2461 cggctttttg tataagtaac ccggtatcta agttcatgga aacaattaac tgtgctataa
     2521 agctttccgg ttgacttaaa agaacataga gaagatgctc ctgctctatc tgtatattac
     2581 cgtattcaat cgcaaggctt tgtgccctgt taagtgcttc tacggatttt tgtgtaaact
     2641 tctgtatatt catcttttcc ctcctgaaat catataatca ttcctgtttt tgcatagtat
     2701 tctttgtatg ccgcatatat atccgatata acataatcgg tattatccat attactcata
     2761 aaaatatcca atgccttatc caatgttgta atatatgccg caatggcttt gccaagcctt
     2821 tgttcttcaa tattcatacg gtcgtttaag aagaaaaatc tcgtatagca gacattttca
     2881 taggatgtca tctccacctc atcatatgcg tcgggtataa gttgcttttc aactttctgc
     2941 catatggtaa aaaagtcttc tatcgctgag ataagactgc tgctttgtgt cctaacctgc
     3001 gctttcaact tgagaaaagg tcctttgtcc cttcccagaa tctccaagca atagcgtatt
     3061 gtaggacggt acctgtagct caatgccgtt cgcacatcc
//
LOCUS       DAOPWC010000159         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32377_length_3098_cov_3.292146, whole genome shotgun sequence.
ACCESSION   DAOPWC010000159 DAOPWC010000000
VERSION     DAOPWC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32377_length_3098_cov_3.292146"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..221)
                     /gene="rpmJ"
                     /locus_tag="PKJ65_04295"
     CDS             complement(108..221)
                     /gene="rpmJ"
                     /locus_tag="PKJ65_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNZ41088.1"
                     /translation="MKVKPSVKKRCEKCKIIKRKGRVMVICDNPRHKQRQG"
     gene            complement(234..452)
                     /gene="infA"
                     /locus_tag="PKJ65_04300"
     CDS             complement(234..452)
                     /gene="infA"
                     /locus_tag="PKJ65_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ41089.1"
                     /translation="MLAKDVIETEGVVIEALPNTMFRVELQNKHVILAHISGKLRMHY
                     IRILPGDKVKIEMSPYDLTKGRIVWRGK"
     gene            complement(475..1140)
                     /locus_tag="PKJ65_04305"
     CDS             complement(475..1140)
                     /locus_tag="PKJ65_04305"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNZ41090.1"
                     /translation="MIVIMLGAPASGKGTQSAIVAQRLKVPHISTGDIFRWNIANNTA
                     VGEEAKKYIDKGMLVPDELTIRILKERIEKEDCSNGFVLDGFPRTINQQQSLDRMLTE
                     CNKSIDMAIDLVVTDDEVIRRISGRRMCKCGKAYHIVTYPPKTPGICDLCNGELYTRK
                     DDTVEVAITRLEAYKLQSEPILAVYRERGIVVDIDGHKPQEQVAEDVWKEILIYSEKH
                     GWM"
     gene            complement(1148..2431)
                     /gene="secY"
                     /locus_tag="PKJ65_04310"
     CDS             complement(1148..2431)
                     /gene="secY"
                     /locus_tag="PKJ65_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ41091.1"
                     /translation="MFQTIRNAWRIPELRKKMLLTIAMLLIYRVGCHLPVPGLIGAAF
                     EVLVEGNKLFGFMDMFSGGAFSNMSLFAMNIQPYINASIILNLLTVAIPALERLAKEG
                     EEGRKKITQYTRYLTIGLGLFQASMLFITLKNYDVLYDGGTVMSFLTVVFTFCAGTAV
                     VMWLGELITEKGIGNGISMIIFVGIVSRIPTAAAVLYANWQQGNLGATAIETILLVTG
                     LLVVMLGMVVGTVYVSGATRKIPVNYAKKVVGRKMYGGQSTHIPMQLNMSGVMPIIFA
                     SSITTLPSIIINFVAPHTENKFLIALQKTDSWGFMAVYAALIFFFSFFYTMISFNPVE
                     VATNLRKNGGFIPGLRPGKPTSDYLAYVLNKITWFGGLFLALLATIPSILAKVVGIQI
                     GFGGSALLIVVGVALETVKQMESQMLMRHYKGFLD"
     gene            complement(2431..2874)
                     /gene="rplO"
                     /locus_tag="PKJ65_04315"
     CDS             complement(2431..2874)
                     /gene="rplO"
                     /locus_tag="PKJ65_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNZ41092.1"
                     /translation="MKLNQLAPAEGSKKKATRKGRGHGSGSGKTCGRGANGQNSRSGG
                     GVRVGFEGGQMPLYRRIPKFGFNNYRFQKNYAEVNIETLNIFKNKTVVDEALLREAGI
                     VKGVCDGIVILGRGELTKNLTVKASKFTKSAAEKITAAGGKAEVV"
     gene            complement(2888..3088)
                     /gene="rpmD"
                     /locus_tag="PKJ65_04320"
     CDS             complement(2888..3088)
                     /gene="rpmD"
                     /locus_tag="PKJ65_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNZ41093.1"
                     /translation="MAKKKAEQAKMLKITLVKSANNSIQKHKATVAALGLRKLGQTVE
                     QKDNEKIRGMIQKVQHLVSVQE"
BASE COUNT          737 a          789 c          548 g         1024 t
ORIGIN      
        1 tttacttttt tacctccata gtcctttcaa aatctttttg agtatctatt ttattactgc
       61 caagaacagt tactcactgt cagctgctta tggcagtgct aagcacatta tccctgtctt
      121 tgcttgtgcc tgggattatc gcagattacc ataactctgc cttttctttt gataatcttg
      181 cacttttcgc atcttttctt tactgacggt tttactttca tagtatatcc ctcctatttg
      241 cctctccata ctatccgacc tttggtcaaa tcatacggtg acatctctat ttttacctta
      301 tctcccggta atatccttat atagtgcatt ctcagtttgc ccgagatatg cgccaatatc
      361 acatgcttgt tttgtaattc cacccgaaac atcgtatttg gtaaagcttc aattactacg
      421 ccttccgtct caataacatc ctttgccaaa atctttgcct ccttatcccg ggcattacat
      481 ccagccgtgt ttttcggagt atatcagtat ttccttccat acatcctccg ccacctgctc
      541 ttgcggtttg tgcccgtcta tatccacaac aattcctctt tctctgtata ccgcaagtat
      601 gggttccgat tgaagcttat atgcctccaa tcttgtgatt gccacctcaa cggtatcatc
      661 ttttctggta tacagttctc cgttacataa atcacagatt ccgggtgtct tgggaggata
      721 ggttacgatg tgatatgctt ttccgcattt acacattctt ctgccggaaa tccttcttat
      781 gacttcatca tccgttacta caagatcaat ggccatatca atggatttat tgcattctgt
      841 aagcatcctg tccaacgact gctgctggtt gattgttctc ggaaaaccgt caagcacaaa
      901 tccgttgctg cagtcttcct tttcaatcct ttccttcaat atacgtattg tcagctcgtc
      961 cggaaccagc attcctttgt caatgtactt cttagcctct tctcccaccg cagtgttgtt
     1021 ggcaatgttc cacctgaata tatcacctgt ggatatatgc ggtactttaa gtctttgtgc
     1081 tactatagcg ctttgtgttc ccttgccgct ggcaggggct cccagcataa taacaatcat
     1141 ggctgcctca atcaaggaat cctttgtaat gcctcatgag catctgcgat tccatttgtt
     1201 taaccgtttc cagtgcaaca cctactacga taagtaacgc actgcctccg aaacctatct
     1261 gtattcctac cacctttgca agtattgacg gtattgttgc caataaagca aggaaaagtc
     1321 ctccgaacca ggttatttta ttcaatacat acgcaagata atcggaagtg ggttttcccg
     1381 gtctcaatcc cggtataaag cctccgttct tccgtaagtt ggttgctact tccaccggat
     1441 tgaaagaaat catggtgtag aaaaaggaga agaagaatat caatgcggca tataccgcca
     1501 taaatcccca cgaatcggtt ttttgcagtg caatcaagaa cttgttttcg gtatggggtg
     1561 ctacgaagtt aattatgatg gaaggcaaag tggttatgga tgaagcaaat attatcggca
     1621 ttacgcccga catattaagc tgcatcggta tatgggtact ttgtcctccg tacatctttc
     1681 ttcccactac tttctttgcg tagtttaccg gtatctttct tgtagctccg gaaacataaa
     1741 cagttcccac taccataccc aacattacca ctaaaagacc ggttaccaaa agtatagtct
     1801 ctatagccgt agctccgaga tttccctgtt gccagtttgc ataaagcact gccgcagcgg
     1861 taggtattct ggatacgata cccacgaaga ttatcatgga tattccgttt cctattccct
     1921 tttccgtaat caattcgccc aaccacataa ctactgccgt acctgcacag aaggtaaata
     1981 ccaccgtcag gaagctcatt accgttccgc cgtcatacag cacatcatag tttttcaatg
     2041 ttataaacag catgcttgcc tggaaaagtc ccagtcctat cgtaagatac ctggtgtact
     2101 gggtaatctt tttcctgcct tcttcacctt ctttagccag tctttccaaa gcgggaatgg
     2161 ctacggtaag aaggttaagt ataatggaag cgttgatgta cggctgtatg ttcattgcaa
     2221 ataatgacat attggagaaa gctcctccgg agaacatatc catgaatccg aaaagtttgt
     2281 ttccttccac cagtacttca aatgccgcac ctataagccc gggaacgggc aggtgacacc
     2341 ctactctgta tatgaggagc attgcgatgg ttaataacat tttctttctc aactcgggta
     2401 tacgccaagc atttctgata gtctgaaaca ttatactacc tctgcctttc ctcctgcagc
     2461 cgtaatcttc tctgcagcgg acttcgtgaa ttttgaggct ttaaccgtaa gatttttggt
     2521 taattcacct cttcccaata taactatacc gtcgcagacg cctttaacta ttccggcttc
     2581 gcgaagcaat gcttcatcaa caaccgtctt attcttaaat atattgagcg tctctatatt
     2641 aacttcagcg taattcttct gaaatctgta attattaaaa ccgaatttag gtattcttct
     2701 gtataaaggc atctgtccgc cttcgaagcc tactcttact ccgccgcccg agcgggaatt
     2761 ctgtccgttg gctcctctgc cgcaagtctt gccgcttccc gatccgtgtc cgcgaccttt
     2821 tcttgttgct ttcttctttg aaccttcggc cggtgctaat tgatttaact tcattttcag
     2881 ctcctcccta ttcttgcaca ctgacaagat gctgtacttt ctgtatcatg cctcttatct
     2941 tctcattgtc tttctgctct acggtttgcc ctaatttacg cagtcccaaa gcggcaacgg
     3001 tagctttatg cttctgtatg gaattgtttg cgcttttgac caacgttatt ttcagcattt
     3061 tagcctgttc agcttttttc ttagccatat agccctcc
//
LOCUS       DAOPWC010000160         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32447_length_3094_cov_2.869036, whole genome shotgun sequence.
ACCESSION   DAOPWC010000160 DAOPWC010000000
VERSION     DAOPWC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32447_length_3094_cov_2.869036"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKJ65_04325"
     CDS             complement(<1..714)
                     /locus_tag="PKJ65_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNZ41094.1"
                     /translation="MKEIRFKSTVFLIMASVLLSRIMGFVRTALIPVKMGGLSRVTDA
                     YNAAFQVPDLMYSMLIGGAIASSLIPVLAGYVAKGEEKKGWRSVSSFMNLVSVIMFVV
                     VILSVIFAKPLVNMLVPGYDAETLELTVQLTKILMPIAFFMMLAGLCNGIMNSYNEFI
                     IPAFGPCLYNLACIISIVLFSNDNPADNYGVKKVVLGIVICAGLYFLFQFAFTFKHFK
                     NNYRTVIAYKEPDFKLLIAL"
     gene            complement(718..1986)
                     /locus_tag="PKJ65_04330"
     CDS             complement(718..1986)
                     /locus_tag="PKJ65_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNZ41095.1"
                     /translation="MKAEIIAVGTELLMGQIDNTDASYMSKRFPECGVYVYYHTVVGD
                     NRGRLAECINIALKRADVVVLTGGLGPTQDDITKETVAEVLGLKPVFHPEILENVKEY
                     FNTIRHNRKINIMAASNEKLAYYPEGCVILPNKNGTAPGCIISHFVEGAEKTVILLPG
                     PPWELQPMFDNYVVDYFKSKIQEGLKSVYLRMFGIGESAMESVISDIVSAQTNPTIAP
                     YAKKGEVMLRVTARYDKGKQDPDLILKPVLDILHERLGEYIYSEENLELKVVTANLLM
                     SKNITVSFAESCTGGMLSELLTDVPGISKVFFGSVVAYSNEIKQSVLNVKSGTLEKYG
                     AVSENTAKEMAQGVRLLYNTEVGISITGIAGPSGAVADKPVGRVYIGISTGRETLSYE
                     YTFSGDRDKVRYFSCLNALNILRLYALSKG"
     gene            complement(1983..2399)
                     /locus_tag="PKJ65_04335"
     CDS             complement(1983..2399)
                     /locus_tag="PKJ65_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNZ41096.1"
                     /translation="MLVKQISVFLENKEGRLAEVTSILAEANININALTIADTADFGI
                     LRIIVDDAVKTEKLLREYGFSVSVTDVLAIDIDNVPGGIARPLIKLHKNGISVEYIYA
                     FLAKEDDTAHVIFKVNNLETAVRILTENGFRNTAKL"
     gene            complement(2410..>3094)
                     /locus_tag="PKJ65_04340"
     CDS             complement(2410..>3094)
                     /locus_tag="PKJ65_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ41097.1"
                     /translation="AGLFGAEPWSENMRKEIERLLNIKAYDIYGLSEITGPGVASECC
                     AKNGLHINEDCYLPEIIDMETQQPLGLDKQGEIVFTTIKKKGMPLIRYRTKDITKLFR
                     DRCRCKRTLVKMERVMGRTDDMLIIRGVNVFPSQIESVLLEFSHITPNYQIVVDRINM
                     MDTLEVRVEMNENLFSDEVRKIELLERNITGAIESVLGISAKVKLTEPKSIERSEGKA
                     VRIIDRRKI"
BASE COUNT          841 a          766 c          530 g          957 t
ORIGIN      
        1 aagtgctatc aacaatttga agtccggttc tttgtatgct attacggttc tgtaattatt
       61 cttgaaatgc ttaaatgtaa atgcaaactg gaaaagaaaa tagagtccgg cacatatgac
      121 tatacccagt actacttttt ttactccgta attgtccgca gggttatcat tactgaataa
      181 tactatgctg ataatacagg ccaggttgta taagcagggc ccgaatgccg gtattataaa
      241 ctcattgtag gagttcatta ttccgttaca aagccccgca agcatcataa aaaaggctat
      301 aggcatcaat atcttggtca actgtacagt taattccaaa gtttccgcat cataaccggg
      361 aaccagcatg ttgaccagcg gttttgcgaa tataacgctt aagattacta cgacaaacat
      421 tattacggag accagattca tgaaagaact gaccgatctc caaccttttt tctcttcacc
      481 ttttgccaca taacctgcta aaaccggaat gagagaggaa gcgatggctc cgccgataag
      541 catggaatac atcaaatcgg gaacctgaaa agccgcattg tatgcatcgg taacccggga
      601 aagaccgccc attttcacgg gaataagcgc ggttcgaaca aatcccatta ttctgcttaa
      661 caggaccgaa gccattataa gaaaaaccgt acttttaaat ctgatctctt tcaaatctca
      721 tcctttcgaa agtgcatata agcgtaatat attcagtgca ttaaggcagg aaaaatatct
      781 taccttatct ctgtctccgc tgaaggtgta ttcataagat aaagtttccc gtccggtact
      841 tatcccgata tacacacggc ctaccggttt atccgcaacg gctccggaag gtcccgcaat
      901 accggttatg gaaataccga cttctgtatt atacagcagc cgtacacctt gtgccatctc
      961 tttagccgta ttctcgctga ccgctccgta tttttccaat gtaccacttt ttacattaag
     1021 aacagattgc tttatttcat ttgaataggc cacaaccgaa ccgaaaaaca cctttgatat
     1081 tcccggtaca tcggtcaata actcggacaa catacctccc gtacacgatt cggcgaaaga
     1141 tacggtaata ttcttactca taagcaagtt tgccgtaacg accttgagtt caagattctc
     1201 ttcggaataa atatactccc ctaacctttc gtgcaatata tccagtaccg gtttaagaat
     1261 taaatcggga tcttgcttac ccttatcgta tcttgcggta acccggagca ttacctctcc
     1321 ttttttcgca taaggagcga tggtcggatt ggtctgggcg gatactatat cggaaataac
     1381 cgattccatt gccgattctc ctataccgaa catgcgaaga tataccgatt tcaaaccttc
     1441 ctgtatctta cttttaaaat aatcaacaac ataattatca aacatgggtt gcagttccca
     1501 cggaggaccg ggaagcaata tgactgtttt ctccgctcct tcaacaaaat gagaaataat
     1561 acatcccgga gccgttccgt ttttattggg cagtattaca caaccctcgg gataatatgc
     1621 cagtttttca ttgcttgccg ccattatatt aatctttcgg ttatgcctga ttgtattgaa
     1681 gtattctttt acattctcca gaatttccgg atgaaaaacc ggtttcagtc ccagaacctc
     1741 agccaccgtc tcttttgtta tatcgtcctg agtaggtccc aatccgcctg taagcaccac
     1801 aacatccgct cttttcagcg caatgttaat acattcagcc aatctccccc gattgtcgcc
     1861 tacaactgta tgataatata catacacgcc gcattcggga aatctctttg acatgtaact
     1921 tgcatcggta ttgtcaatct gccccatcag cagttccgtg cctaccgcaa taatctccgc
     1981 tttcataatt ttgccgtatt cctgaatccg ttttcggtaa gtatcctgac tgccgtctca
     2041 agattattaa ccttaaatat gacatgtgcg gtatcatctt ctttagccaa gaaagcataa
     2101 atgtactcca ccgatatccc gttcttatga agctttatca gcggacgtgc tatgcctccc
     2161 ggcacattat cgatatctat tgccaaaaca tcggttacac ttacggaaaa accgtattcc
     2221 ctcagcaatt tttccgtttt tacggcatcg tcgacaataa ttcttaatat gccgaaatcc
     2281 gcggtatccg caatggtaag tgcgttgata ttaatatttg cttcggccaa tatcgaggtc
     2341 acttcggcaa gtctgccttc tttattttcc agaaataccg atatctgttt aactaacatt
     2401 tcacacccct taaatctttc ttctgtcgat tatacgtaca gcttttcctt cacttctttc
     2461 aattgatttg ggttccgtta atttaacttt ggcactgata cccaatacac tctcaatcgc
     2521 tccggttatg ttcctttcca ataactcaat cttcctgacc tcatcggaaa acagattctc
     2581 attcatttcc acccgcactt ccagtgtatc catcatgttg attctgtcca ctacaatctg
     2641 ataattggga gttatgtgac tgaattccag caatacgctc tcaatctgcg aaggaaatac
     2701 atttactccc cttatgataa gcatatcgtc ggtcctgccc attacacgtt ccattttaac
     2761 cagtgttctt ttgcatcggc aacggtcacg gaataattta gttatatctt tcgtcctgta
     2821 tctgattaaa ggcattccct ttttctttat ggttgtgaaa acaatctcgc cctgcttatc
     2881 caatcctaaa ggttgctgtg tttccatatc tattatttcc ggtaaataac agtcttcatt
     2941 gatatgcaga ccgttctttg cacaacattc cgatgcgaca cccggtccgg ttatctcgct
     3001 taagccgtat atgtcgtaag cttttatatt cagaaggcgt tctatttcct ttctcatgtt
     3061 ctcgctccac ggctccgctc cgaaaagtcc cgcc
//
LOCUS       DAOPWC010000161         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32595_length_3083_cov_2.504293, whole genome shotgun sequence.
ACCESSION   DAOPWC010000161 DAOPWC010000000
VERSION     DAOPWC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32595_length_3083_cov_2.504293"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2697)
                     /locus_tag="PKJ65_04345"
     CDS             complement(<1..2697)
                     /locus_tag="PKJ65_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ41098.1"
                     /translation="MNSIDLFAAIADTKIEQRKEYMEAKQPVPVPDVLFECVNIVKDN
                     DICKDNNEGKVIDKIKDKYSFPEEQSYEQQLKDYYEGLEQLKAQYAPFLKRRSKHAIK
                     RTRLEFENFNFRFETREDIDDFSAVLEGRGIWESITIPHYHGPTGKWVTYYRTEFDYK
                     KDNLRDYIVFKGSDYITGVYVNGRHVLSHEGFFYAFEEDITEYLKEKGNVLVVRVEND
                     ITMLGENGRLGKKIYAATGIGWDDPKDGWHHCPPGGGIFDKVYIEKRESTFIFDACVY
                     PDIDNQTAKIKVVVKNVDRYKKDVKINISVEPFNFDDCFRYTGEFQRKIHEGENHYHF
                     DIKIENPKLWHSFTPYLYTVIIESDNSDQYEQNFGMRKFHMDTENKPYGTLYLNNTEI
                     LLRGANEMGHLQLCVMNDDYEQLIEDILIAKYCNMNYYRITQRPVQSEIYEYCDMLGM
                     MVQADFPLFGQLARSQFYEAVRQVGEMERHVRRHPSVIMVSYINEPTDQFKSDNQQIN
                     LNRAELENWFEICDRVIKQENPYRVIKRVEGDYDPPTFEGLSDFHCYNMWYTNHALPI
                     GDLYKGYLPAIRKDWKTACGEYGTEGLDNLDLMKERYPKEWLPKNEEDIWIPDRIVRA
                     QTNSMHGDWFYEQDNIKDWIYFSQLHQAKAASIMTLALRRRSDYVVQTALHLLIDAYP
                     SGWMKTVAGVDRVPKPAYFTFKEALIPLKVNLRCDRRTCYTNDVLNAECWILNDTAVD
                     YSDMTITAVLYDRHKNPLKTYKLNTSAKGCKSVPVGKIRIDTSDIAVKGRQTFSLAVF
                     MEHQGKIIDTDVYNFDIFENNYESINAYPLGMLAKQVVNTSPAFKQADKETADIIIIS
                     EKKHLKEAEAIKKSINKPILFILNSRLENFKFNRHTYERSDRR"
     gene            complement(2710..>3083)
                     /locus_tag="PKJ65_04350"
     CDS             complement(2710..>3083)
                     /locus_tag="PKJ65_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ41099.1"
                     /translation="AYRNYYDIWQSYPDYDSVKVWEKDNLYSNMVYDAVYKMSKCAFE
                     IGEYNIAIKGYTFVEDSLSEFAKANREGLIYHSAKAYAGIEDYVKAEELFKKVIDEYP
                     ASNLAIYAKDGLAAVRKKLGR"
BASE COUNT          801 a          705 c          503 g         1074 t
ORIGIN      
        1 ccttctgtcg ctcctctcgt aagtgtgtcg gttaaactta aaattctcca gacgggaatt
       61 cagaataaat aagattggct tattaatgct tttcttgatc gcttccgctt cctttaaatg
      121 ttttttttcg gaaataatta taatatctgc cgtttcttta tctgcctgct taaaagcggg
      181 gcttgtattt acaacctgtt ttgcgagcat tcctaaggga taagcattga tactttcata
      241 attattttca aagatatcaa aattatacac atcggtatca attatcttgc cctgatgctc
      301 cataaatacc gctaatgaaa atgtctgtct tccctttacg gcaatatccg atgtgtcaat
      361 gcgtattttt ccgacgggaa ctgatttaca accttttgcg gaagtattca atttgtaagt
      421 tttcaagggg tttttgtgtc ggtcatataa cacagccgtt attgtcatat cgctgtaatc
      481 aacggcggta tcgtttaaaa tccagcattc cgcattcagt acatcgtttg tataacatgt
      541 tcttctgtca catcgcaaat tgactttaag aggaatcagg gcctctttaa aagtgaaata
      601 tgcaggtttc ggcactctgt ccacacccgc tactgtcttc atccaccccg aaggataagc
      661 gtcaatcaga aggtgcaatg cggtctgaac cacataatcg cttcttctcc ttaatgccag
      721 agtcataata cttgcagctt ttgcctgatg gagttgactg aaatatatcc agtcttttat
      781 attatcctgc tcatagaacc agtcaccgtg catggaattg gtctgtgctc ttactatcct
      841 gtccggtatc cagatatctt cttcattctt cggaagccat tctttagggt atctttcttt
      901 cataaggtca agattatcca acccttcggt cccgtactct ccgcatgccg ttttccagtc
      961 ttttctaata gcgggcagat aacctttata taaatctcct atcggtagag catgatttgt
     1021 ataccacatg ttatagcagt ggaaatcgga taagccttca aaggtaggag gatcgtaatc
     1081 cccttccacc cttttaatca ctctgtaagg attttcctgc tttattactc tgtcacatat
     1141 ctcaaaccaa ttctccagtt ccgctctgtt aagatttatt tgctgattgt ccgatttgaa
     1201 ctgatcggta ggctcattta tgtaggaaac cattattacg ctcggatgtc tccttacatg
     1261 tctttccatc tcgcctacct gtcttaccgc ttcataaaac tggcttcttg caagctgacc
     1321 gaataacgga aaatccgcct gtaccatcat tcccagcata tcacagtatt catatatttc
     1381 cgattgcacc gggcgctgcg taatcctgta ataattcata ttgcagtatt tggctataag
     1441 gatgtcctct ataagctgct cataatcatc attcattacg cataactgca aatggcccat
     1501 ctcatttgca cctcttaaca gaatctcggt attgttaaga taaagcgttc cgtacggttt
     1561 attctccgta tccatatgga atttacgcat tccgaaattc tgctcatatt gatcggaatt
     1621 gtccgattct attatcacgg tatacagata cggagtaaaa ctgtgccata atttggggtt
     1681 ttctattttg atgtcaaaat ggtagtggtt ttccccttca tgtatttttc tttgaaactc
     1741 acctgtatat ctgaagcaat cgtcaaaatt aaacggttca acagaaatat ttatcttaac
     1801 atccttctta taccgatcaa catttttaac tactactttt atctttgccg tctgattgtc
     1861 tatatccgga taaacacagg catcaaaaat aaatgtactt tctctttttt caatatatac
     1921 cttatcaaaa ataccgcctc cggggggaca atgatgccat ccgtccttcg ggtcatccca
     1981 accgataccg gttgcggcat atatcttttt acccaatctg ccgttttccc ctaacatggt
     2041 gatatcgttt tccactctta ctacaagaac atttcccttc tctttaagat attccgtaat
     2101 gtcttcttca aacgcataga agaaaccttc atgcgacagt acatgtcggc cgttgacata
     2161 taccccggta atgtagtcgg aacctttgaa tacgatatag tctcttaagt tgtctttttt
     2221 gtaatcaaat tccgtccggt aatatgttac ccatttaccc gtaggaccgt ggtaatgagg
     2281 tattgttatg ctttcccata taccccttcc ttccaaaacg gctgagaaat cgtcaatatc
     2341 ctcccgtgtc tcaaaacgga aattgaaatt ctcaaactcc agtctcgttc tttttattgc
     2401 atgtttactt cttcttttca gaaaaggtgc gtattgcgct tttaattgct caagaccttc
     2461 atagtaatcc tttaattgct gctcgtacga ttgctcctcg ggaaaagaat atttatcttt
     2521 tattttatca atcactttgc cttcattgtt atctttacaa atatcattgt ctttaacaat
     2581 attgacacac tcaaacaaaa catccggtac cggtaccggt tgctttgctt ccatatattc
     2641 ttttctttgt tctatctttg tatcggcgat tgccgcaaac aaatcaatac tgttcaaagc
     2701 atacctctgc tatcttccta atttctttcg gacagcggca agtccgtctt ttgcataaat
     2761 tgcaagattg gaggcgggat attcgtctat taccttctta aaaagttctt cggcttttac
     2821 atagtcttct attcccgcat atgcctttgc actgtgatat atcaaacctt ctctgtttgc
     2881 cttggcaaat tccgaaaggc tgtcctcaac aaatgtatat ccttttatag caatgttata
     2941 ttcacctatc tcgaaggcgc atttggacat tttataaacg gcatcatata ccatattgga
     3001 atagagattg tccttctccc atactttaac cgagtcatag tcgggataac tttgccatat
     3061 atcataataa ttcctgtatg ctt
//
LOCUS       DAOPWC010000162         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32840_length_3066_cov_3.166058, whole genome shotgun sequence.
ACCESSION   DAOPWC010000162 DAOPWC010000000
VERSION     DAOPWC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32840_length_3066_cov_3.166058"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKJ65_04355"
     CDS             <1..496
                     /locus_tag="PKJ65_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41100.1"
                     /translation="KLDFDKYENMLYYLTFSSWSLQRSNFVQCLNEETVDFKNLKKLT
                     RPNQMASDAQRACKYAKLMDNPILVSEAQATYSAIEAYDYTRKLLKLSSRKNPDRNRY
                     IVYKERLNEACKAVYTALMEWGENMKQAGAYDTYIHETAKAFLHLPSGFNRFAAEAEK
                     TSEE"
     misc_binding    536..735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            777..>3066
                     /gene="alaS"
                     /locus_tag="PKJ65_04360"
     CDS             777..>3066
                     /gene="alaS"
                     /locus_tag="PKJ65_04360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNZ41101.1"
                     /translation="MEKLSLNTLREEFLKFFESKGHLRLQSFSLIPEDDPSILLINAG
                     MTPLKPYFTGTLKPPSKRITTCQKCIRTLDIDRVGITTRHGTFFEMLGNFSFGDYFKK
                     EAIQWSWEFFTEVLKMDKNLLYASVYEEDDEAYDIWKNIVKLDESHIVRMGKEDNFWE
                     HGTGPCGPCSEIYYDRGEDKGCGSKDCKVGCDCDRFVEVWNNVFTQFDKQEDGTYKPL
                     KNKNIDTGMGLERLACVVQGVDSIFEVDTVKDILDFVCNATDTEYGKDKTKDISIRVI
                     TDHIRSSVMMISDGIIPSNEGRGYVLRRLLRRASRHGRLLGKKDAFLSELIDVVINNS
                     KSAYPELEEKRDYILKVVQSEEAKFINTIENGMNILNQYLEEAENNKKDTLAGEHIFK
                     LHDTYGFPYDLTREIASERGFKLDKEGFDEYMQNQKKTARDAFKADEGSAWHSDENIY
                     KNIPKTEFSGYEKLEIKAEVLSFVKKDETAELVTDKTVFYAEGGGQQADIGVITGKKA
                     KMTVTDVINREGIFIHRGVMEQGNFSDDETVTLKVDKSRRKATERNHTATHMLHYALR
                     EVLGNHVHQAGSYVDHQRLRFDFTHFSAMDDRQLMKVVSIVNNIIMEDIDVVKEIMDM
                     DTAKKKGATALFGEKYSDKVRVVSIHPYSMELCGGTHLSKTGETGLFTIVSEGAVASG
                     VRRIEALTGANAIEYMQKLEDTVNQLNRMLKGSDVIARVENLSSTVRELRKENEELKN
                     KLSANKLDNVLNTFEQYKNIKIV"
BASE COUNT         1059 a          519 c          722 g          766 t
ORIGIN      
        1 aaagctggat ttcgataaat atgaaaatat gctgtattac cttacgtttt cttcctggtc
       61 attacaaaga agtaactttg tgcagtgctt aaacgaagaa accgtggact ttaagaatct
      121 taagaaactg acgcgtccca accagatggc aagtgatgcc caaagggctt gcaaatatgc
      181 aaaacttatg gataatccga tacttgtaag tgaggctcaa gccacatatt cggctataga
      241 agcatatgat tacacaagga aacttctgaa gttatcttct cgtaaaaacc ctgacaggaa
      301 cagatatatt gtatacaaag agcggctgaa tgaagcatgc aaagcggtct ataccgccct
      361 tatggagtgg ggagagaata tgaagcaagc cggagcatat gatacatata tacacgaaac
      421 cgcaaaagct ttcctgcatc taccgtccgg atttaacaga tttgctgccg aggcggaaaa
      481 aacaagcgaa gagtagctat attgacatac attgaataaa tgatataatt tcctcaatac
      541 ggcgttacta aaaggatgag taacatatgg tctgtggcac agagagaggg gaacggtgag
      601 aacccttgca agaatatatg tgaaagctgc cctttgatgc cttccgcaaa acggaacgta
      661 tacctgcgtt aaaggtttaa gagctttctt aggaaagaat ttgggtggta ccgcgtgtta
      721 actctcgtcc ctttgcagga tgggagttat ttatttatat aaggatggtg atagaaatgg
      781 agaaattatc attaaacaca ttgagagaag agttcctgaa attttttgaa agtaaagggc
      841 atctcaggtt gcagagtttt tctctgatac ccgaagacga cccctccata ctgcttatta
      901 atgcgggaat gacaccgcta aaaccgtact ttaccgggac tttaaaaccg ccgtctaaaa
      961 ggataactac ctgccagaag tgcataagaa ctttggatat cgaccgtgtg ggaattacca
     1021 caagacacgg aacatttttt gagatgctgg gtaatttctc tttcggcgat tactttaaga
     1081 aagaggcaat tcaatggtcg tgggagtttt ttaccgaagt tctgaaaatg gataagaatc
     1141 ttctctatgc atcggtctac gaagaagacg acgaagcata tgatatctgg aaaaatattg
     1201 tgaaactgga tgagagccat attgtaagga tgggtaaaga agataatttc tgggagcacg
     1261 gaacaggacc ttgcggaccg tgctcggaaa tttactatga cagaggagaa gataaagggt
     1321 gcggttcaaa agactgtaag gtaggctgtg actgcgacag atttgtagag gtatggaata
     1381 acgtatttac acaattcgac aagcaggaag acggcactta caaaccttta aagaataaaa
     1441 acattgatac cggtatggga ctggagcgat tggcctgtgt ggtacaggga gtggattcga
     1501 tatttgaagt ggataccgtt aaagacatat tggattttgt ctgcaatgct accgacactg
     1561 aatacgggaa agacaagaca aaggatattt ccatccgcgt cataaccgac catataagaa
     1621 gttccgttat gatgatatcc gacggtataa taccttcaaa cgaaggcaga ggctatgtct
     1681 taagaagact gttgagaaga gcatccagac acggaagact gttaggtaaa aaagatgctt
     1741 tcctttcgga acttattgat gttgttatta ataattccaa gagtgcatat ccggaactgg
     1801 aagaaaaaag agactatata ttaaaagtgg tacaaagtga agaagccaaa ttcattaata
     1861 ccattgaaaa cggaatgaac attctaaacc agtaccttga agaggctgaa aacaacaaga
     1921 aagatactct tgcaggtgag cacattttca aacttcacga tacatacggt tttccctatg
     1981 atcttaccag agaaatcgct tcggagagag gatttaaact ggataaggaa ggttttgacg
     2041 agtatatgca aaatcagaaa aaaactgcca gggatgcttt taaagcagat gaagggtctg
     2101 catggcacag tgatgagaat atatacaaga atatacctaa aacggaattt tcaggttatg
     2161 aaaagcttga aataaaagct gaagttttat cattcgtaaa gaaagatgaa acggcggaac
     2221 tggtaaccga caaaacggta ttctatgccg aaggcggcgg tcagcaggca gatatcgggg
     2281 ttataaccgg caaaaaggca aagatgacgg ttaccgatgt gataaacaga gaaggcatct
     2341 ttattcacag aggtgtaatg gagcaaggca acttcagtga tgatgaaaca gtgactctta
     2401 aagttgataa atcaagaaga aaagctaccg aacgaaacca tacggctact catatgcttc
     2461 actacgcatt aagagaagta ttgggtaatc atgttcatca ggccggttcc tatgtagatc
     2521 atcagagact gagatttgac tttactcatt tttccgctat ggatgaccgg cagcttatga
     2581 aagttgtgag tatagtgaac aacattatca tggaagatat tgatgtggtg aaagaaataa
     2641 tggatatgga cacggctaag aaaaaaggcg ccacggcatt attcggagaa aaatactccg
     2701 ataaagtacg tgtggtatcc attcatcctt acagtatgga actgtgcgga ggcacgcatc
     2761 tttcaaaaac gggagaaaca ggcctgttta caatagtatc ggaaggtgca gtagcttccg
     2821 gtgtacgaag aatagaagca ttgacaggtg caaatgcaat tgaatatatg caaaagctgg
     2881 aagatacggt taatcagctt aacaggatgc ttaaaggaag cgatgttata gccagagtgg
     2941 aaaatctttc ttccaccgtc agggagttaa gaaaagaaaa cgaggagttg aagaataagc
     3001 tttccgcaaa caaactggac aatgttctta acacattcga gcagtataag aatataaaga
     3061 tagttt
//
LOCUS       DAOPWC010000163         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_32880_length_3063_cov_3.061835, whole genome shotgun sequence.
ACCESSION   DAOPWC010000163 DAOPWC010000000
VERSION     DAOPWC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32880_length_3063_cov_3.061835"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..2361
                     /locus_tag="PKJ65_04365"
     CDS             163..2361
                     /locus_tag="PKJ65_04365"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HNZ41102.1"
                     /translation="MKNNISHYDYRAQLPDSYFKFIPLGNGRTGASVWVTAQDTTDIH
                     LLLSATDNFSELGRLLKTCKAYISITPSVFNGNSETHLDLKKALLTIKSDKAEMKMYI
                     DANYDVLCFSLKSDIPVSIKYGIDNYRDKPLSSHDMEEDFSNYILSGTARFHNISESA
                     DCVKVSENKKGLTQYHRNDISFFEYAYFAQNLYSEDKDVFRDPYIGLTFGTYAVSEEL
                     RPVNKGKSSFLETEKPVCDCNIRIWSLTEKADSADAWEKKIIDLAKSFDCGKSFKCHV
                     TDWEDYWNRYYIYAYGTEDAVLLTRAWIYQKYFNGCAGKGPVPIKFNGSIFVTYPHEV
                     TPGRITWDYRRWGGAFWMQNTRHIYWNMLLSGDFSGMEPLYNLLHQLIPISKHRTFAY
                     YGHNGILLPETFTLCGLYCMSNYGIPQEDGKRYAKGTWLHQPGQIANHYIRWYYTSML
                     ELAYIMLLGARYSDNEKYLNIAYEFSKETLIFYYEHFDTLDGKILLKPISSLETYQDC
                     FNDLPNIAGLDALTEELLSGDNLPSDIFDICKKIRDILVDYPKDDNKLLPCQILIDKK
                     RRNCENPELYSVFPFYVHTYFNKEHPLFKYAVNAFYQRNEKAGKGWSQDCIQAALLGL
                     TDQAKDILVKQAKMIDERYSFTGIYGPNYDETPDQDHANGLSIGFMHTLVASSKDKNV
                     LFGAWPEDWNVSFRLPLYSSKPVTAVYQNGKVVTLEFEDEQDRIKTEVHI"
     gene            complement(2418..>3063)
                     /locus_tag="PKJ65_04370"
     CDS             complement(2418..>3063)
                     /locus_tag="PKJ65_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008012265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ41103.1"
                     /translation="YKNKKWAENNKEYFMCFAAGVLITAPLITAFPEALAKNHYAGFA
                     ALAGFMFMLFTNKLIKHHTGQEELAFGITAVQGIGIHSLLDGITYTVTFSAGIFTGLA
                     SGIGLVVHEFTEGIITFSVLLKGGISAKKAALYAFLVAGLTTPVGAFVAYPLVRKLSD
                     SVLGLALGFVVGVLVYISAAHLLPEATSHEKKHSMVALCLGMALSLGLSFLHHH"
BASE COUNT          969 a          589 c          666 g          839 t
ORIGIN      
        1 tcctttaatt gaagaagaag ttgtctatcg tgtgggtaat gttaaatcac ctactgcggt
       61 tataaagttg aaggagaata ataaaaaatg aggtgtagac cttgctttgt tgtacataat
      121 gtaacagaag ggtatggtat aatactctaa aggagaccaa ctatgaaaaa caatatttcc
      181 cattatgatt acagagcgca attacccgat tcctacttta agtttatacc tttgggaaac
      241 ggtagaacag gagcttcggt gtgggttacc gcacaggata ccaccgatat tcatctgttg
      301 ttgtcggcaa ccgataactt ttccgaattg ggcagattat taaaaacatg caaagcatac
      361 atctctatta ccccgtctgt ttttaacggc aattcggaaa cacatctgga tttgaaaaag
      421 gcgttgctta caattaaatc ggataaagcg gaaatgaaga tgtatataga cgcaaattac
      481 gatgtgttgt gtttttctct gaaatcggat attccggtaa gtattaaata cggtattgat
      541 aattacagag ataagccgtt aagctcgcat gacatggagg aagatttcag taattatata
      601 ttgagcggta ccgcaagatt ccataacata tcggaaagtg ccgattgtgt gaaggtatcg
      661 gaaaacaaaa aaggtcttac tcaatatcac agaaatgata tttccttttt cgagtatgca
      721 tactttgccc agaacctcta ttccgaggat aaggatgtat tccgcgaccc gtatatcggt
      781 cttacattcg gaacatatgc ggtatcggag gagctgagac cggttaacaa agggaagtcc
      841 tctttcttgg agacggaaaa accagtgtgt gactgtaata tccggatatg gtctttaacg
      901 gaaaaagccg attcggctga tgcttgggaa aaaaagataa ttgatttagc aaaaagtttt
      961 gactgcggta agagttttaa atgtcatgtt accgattggg aagactactg gaaccgctac
     1021 tacatatatg catacggaac ggaagatgcc gttttgctga caagggcatg gatatatcag
     1081 aaatacttca acggctgtgc cggaaaagga ccggttccta taaagttcaa cggctctatt
     1141 tttgtcacct atcctcacga ggtaacaccg ggaaggataa cctgggatta tagaagatgg
     1201 ggaggggctt tctggatgca gaatacacgg catatttact ggaatatgct gttaagcggc
     1261 gacttttcgg gaatggagcc gttgtacaat cttttacatc aattaatacc catatccaag
     1321 cacagaacat ttgcctatta cggacataac ggaatactgc ttcccgagac tttcacattg
     1381 tgcggacttt actgcatgag caactacggt atccctcagg aagacggtaa aaggtatgca
     1441 aaaggcacat ggctgcatca gcccggacag atagccaacc actatattcg ctggtactat
     1501 acaagcatgc tggaattggc atatattatg ctgttaggtg caagatattc ggacaatgaa
     1561 aaatatctga atatagcata tgagttttcc aaagaaaccc ttattttcta ctatgagcat
     1621 tttgacactc ttgacggcaa gattctctta aaacctatat catcattgga aacataccag
     1681 gattgcttta acgatttgcc caatatagcg ggactggatg ctttgaccga agagttgtta
     1741 agcggtgata atcttccttc ggatattttt gatatatgca aaaagataag ggatatactt
     1801 gtagactatc ccaaggatga caacaaactg ttgccttgtc agatattgat tgataagaaa
     1861 agaagaaatt gcgaaaatcc cgagctttac agtgtatttc ctttctatgt ccacacatac
     1921 tttaataaag agcatccgct tttcaagtat gccgtcaatg cattttacca aaggaacgaa
     1981 aaagcgggga aaggctggtc gcaggactgc attcaggccg ctttattggg tcttaccgat
     2041 caggcaaaag atatactggt aaagcaggca aaaatgatag atgagagata ttcctttacc
     2101 ggaatatacg gtcccaacta tgatgaaact cccgaccagg atcatgcaaa cggattgagt
     2161 atagggttca tgcatacgct tgttgcctcc tcaaaggaca agaatgtact gttcggagcc
     2221 tggccggaag attggaacgt atctttcagg ctgccgcttt attcgtcaaa acccgtaacg
     2281 gctgtttacc aaaacggtaa agtagttaca ctggaatttg aagatgagca ggacagaata
     2341 aagacagaag tgcatatata gccgtttatg tttattgaac ggcaggttta attctatttt
     2401 actgaacaga tacagtatta atgatgatgt aaaaaggaca atcccagtga taatgccata
     2461 cctaagcaca atgcgaccat tgagtgtttc ttttcatgcg atgtcgcttc gggtaacaga
     2521 tgagctgccg atatgtatac cagtacacct actacaaatc ctaaagcaag acccaatacg
     2581 gaatcactta acttccgcac caaaggatat gccacgaatg ctcctacggg agtggttaat
     2641 cccgcaacaa gaaaagcata aagtgcggct ttttttgcac tgataccgcc ttttaacaga
     2701 acggaaaagg taatgattcc ttcggtaaac tcatgtacca caagaccgat acccgatgca
     2761 agaccggtaa atatgccggc actgaaagtg accgtatatg ttattccgtc cagcaacgaa
     2821 tgtatgccta ttccctgaac ggcggtaata ccgaaagcca attcttcctg accggtatga
     2881 tgttttatta atttattggt aaaaagcata aacatgaaac ctgccagagc ggcaaatccg
     2941 gcataatgat tctttgcaag cgcttcggga aaagcggtta taagaggagc ggttattaag
     3001 acacctgcag caaagcacat gaaatattct ttgttattct ccgcccattt cttgttttta
     3061 taa
//
LOCUS       DAOPWC010000164         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_33951_length_2998_cov_2.958206, whole genome shotgun sequence.
ACCESSION   DAOPWC010000164 DAOPWC010000000
VERSION     DAOPWC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33951_length_2998_cov_2.958206"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKJ65_04375"
     CDS             <1..241
                     /locus_tag="PKJ65_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41104.1"
                     /translation="ETMKEPDMVAFTSVSGEWGKAGLKYVSDCMVEGIDPQDALKAYL
                     TVVMGFNPQAILDEMNSRLGKTSEQNYNAIWSKFN"
     gene            539..1219
                     /gene="rpsB"
                     /locus_tag="PKJ65_04380"
                     /pseudo
     CDS             539..1219
                     /gene="rpsB"
                     /locus_tag="PKJ65_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            1465..2112
                     /gene="tsf"
                     /locus_tag="PKJ65_04385"
     CDS             1465..2112
                     /gene="tsf"
                     /locus_tag="PKJ65_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ41105.1"
                     /translation="MISAGLVKELREKTGVGMMECKKALVEANGDLDKAVNILRERGL
                     ANAEKKSGRIAAEGVVASYIHGGGRIGVLVEVNIETDFAAKNEDFLTYVKDIAMQIAA
                     SKPEYIKRDQVPAEVIENEKAILRTQALNEGKPEKVVDKMVEGRIEKYYKQVCLMDQA
                     YIRNPDISVQQLTNEIIAKIGENISVRRFTRYEMGEGLQKREENFADEVAKQMNK"
     gene            2155..2856
                     /gene="pyrH"
                     /locus_tag="PKJ65_04390"
     CDS             2155..2856
                     /gene="pyrH"
                     /locus_tag="PKJ65_04390"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ41106.1"
                     /translation="MNYKRILIKLSGEVFAGPKGENLDTKMLMQIAQALKMLKDDGKQ
                     IAVVVGAGNFIRGRSIKDIVRSRADSMGMLATVINSIALMDALEKTGLDAEVYSAISM
                     SEICKTYRRDDAVRDLGNGIITILAGGTGNPYYSTDMAAVLRAIEINADVILFAKNVD
                     GVYDKDPKEHKDAVKYDKVTYSTILSRQLNVIDMAAAVMCNEFSVKSVLFALKKPMNI
                     VEAARGVNIGTIIEK"
BASE COUNT         1101 a          436 c          706 g          755 t
ORIGIN      
        1 tgaaaccatg aaagagcccg atatggtggc attcacatcc gtaagcggtg agtggggtaa
       61 agcaggtctg aaatatgttt ccgactgtat ggttgaaggc attgacccgc aggatgcttt
      121 gaaggcatat ctcacagttg taatgggatt caacccgcag gcaatactgg atgagatgaa
      181 ctcaaggtta ggaaagactt cagagcagaa ctacaatgct atctggtcta agttcaacta
      241 aacaacaaaa aaaagaaaag actcgggaaa ccgggtcttt tttttatcaa aaaattttta
      301 tatatttttt atttaaaaat atttattaat aaatactatt tatattcttg tagttaaaat
      361 aaattgaaaa agtaaattcc agtactaata ttattctgat ataaatcggt cttgcataat
      421 atcaaatgat aatatataat tcattggaaa ataacacatg tcggtgaatt tatgtatgtt
      481 gcgttcaacg ttgtacatga agatgaagcc ggcagaggaa aaaacggagg attaataaat
      541 gtcagttatt tcaatgaaac aactattgga ggccggagtt catttcggtc atcagacaag
      601 aagatggaac cccaaaatgg cagagtacat cttcacggaa agaaacggta ttcacattat
      661 cgatttacag aagaccgtaa aacaaataga aaaggcatat gattttgtta aaaaagtagt
      721 tcttgacggc ggagaagtat tatttgtggg aacaaaaaaa caggcacagg aatgtgttga
      781 ggaagaagca atcagatgcg gtatgttcta tatcaacaca agatggctgg gcggaacttt
      841 gaccaactac aagacaatgg agaaaaggat tgcctatctt aagaaacttc acgagatgga
      901 gcaagacggc acatttgatt ctttacccaa aaaagaagta ataaagctga gagcggaaat
      961 ggaaaagctg gaaaagaacc ttggaggaat caaagatatg cacggaattc ccaatgtact
     1021 ttttgttgtg gacccgaaga aagaacataa tgcggtacag gaagcaaaga aactggatat
     1081 tccggtagtg gcaattatag ataccaattg cgatcccgat gaagtcgatt atgtaattcc
     1141 gggaaatgac gatgctataa gagcggttaa acttattacc agtgtaattg ccaatgccgt
     1201 tatagaagcg aatcagggtg aagccttctc ggtggaagat gaagaagata ttgatatgtc
     1261 tcaactgacc gaagaagagc ttttaaagac aaaacatgat gacaaaaaaa atgatgacca
     1321 taaaaaagaa tctaaaaaag ccgcaaaaaa ggccgaatct ttaactgaag cagtgcagga
     1381 agcggaagta cttgccgagg agataacgga agttgcagag caggcaattg aaattgcgga
     1441 agaataatac atggaggtta tgcgatgata agtgcaggtt tagttaagga attacgtgaa
     1501 aaaaccggtg tcggaatgat ggagtgcaag aaagctcttg tggaagctaa cggtgatctg
     1561 gataaagccg tcaatatctt aagagagaga ggattggcta atgcggaaaa gaaatccgga
     1621 agaattgccg cagaaggtgt tgtcgcatca tacattcatg gcggcggaag aataggtgta
     1681 cttgtggaag ttaatataga aacggatttt gccgcaaaga atgaagattt tcttacatat
     1741 gtaaaggata ttgcaatgca gatagcggct tccaaaccgg aatatataaa gagagatcag
     1801 gttcccgcgg aagttatcga gaatgaaaaa gcaattcttc gtactcaggc gttaaatgaa
     1861 ggtaagccgg aaaaagttgt agataaaatg gttgaaggaa gaatagagaa gtactacaaa
     1921 caggtatgtc ttatggacca ggcatatatc aggaatccgg atatctccgt acagcagttg
     1981 accaatgaaa ttattgcaaa aatcggagaa aatatttcgg taagaagatt taccagatat
     2041 gaaatgggag aaggtcttca gaagagagaa gaaaattttg ccgatgaagt ggcaaaacaa
     2101 atgaataaat aagtaataaa ggcactaaat gtgcctttta aattggagaa tgctatgaat
     2161 tataaaagga tactgattaa actgagcggt gaagtttttg ccggacctaa aggagaaaac
     2221 ctggatacga aaatgcttat gcagattgca caggcattga agatgctgaa agatgacgga
     2281 aaacaaatag cggttgttgt aggtgccgga aattttataa gaggaagaag cataaaagac
     2341 attgtaaggt ccagagcgga ttcaatgggt atgttagcaa ccgttataaa cagtatagca
     2401 ttgatggatg cactggaaaa aaccggtctg gatgcggaag tatattcagc tataagtatg
     2461 agtgaaattt gcaaaacata cagaagagat gatgcggttc gtgatcttgg taacggtatc
     2521 ataactatac tggcaggagg cacgggaaat ccgtattatt caaccgacat ggcggcggta
     2581 ttgcgtgcta tcgaaataaa tgccgatgtt atactttttg ccaagaatgt tgacggtgta
     2641 tatgataaag atcccaagga gcataaagat gcggtcaaat acgataaggt aacttattcg
     2701 acaatcctgt caagacaact taatgtcatt gatatggctg ctgccgttat gtgcaacgaa
     2761 ttttccgtga aatcggtctt atttgctctt aaaaaaccca tgaatatagt tgaagccgca
     2821 agaggggtaa atatagggac cattattgaa aaataacttc atattgtgta aaatatatct
     2881 aatctattaa acataaagga gttattgcca tgattagtaa agatgaatac agtaaaacta
     2941 cggaattgat gtctcaggca attgatgcat ttagcgaaca gatttcagtt atacgagc
//
LOCUS       DAOPWC010000165         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_33952_length_2998_cov_2.686714, whole genome shotgun sequence.
ACCESSION   DAOPWC010000165 DAOPWC010000000
VERSION     DAOPWC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33952_length_2998_cov_2.686714"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKJ65_04395"
     CDS             complement(<1..517)
                     /locus_tag="PKJ65_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNZ41107.1"
                     /translation="MSINLVPMPNEFHTDYKLFFRRNILESITVEYDSEYSAEEYSIV
                     IDAKGATIKCSTKAGENYAHQTLRQLEFGYSGNIPYLTIHDKPAFSHRGFLIDCCRHF
                     FTVEELKKMIDAAALFKFNKFHWHLTEDQGWRMEIKKYPLLTEKGSQRPYSNFGRYYN
                     PKPYGGFYTQEE"
     gene            complement(621..2162)
                     /locus_tag="PKJ65_04400"
     CDS             complement(621..2162)
                     /locus_tag="PKJ65_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41108.1"
                     /translation="MLQIKDLSIYMKNDGRPLVERLTINLNSNDKVAVIGEEGNGKST
                     LLRAIYDVESISDYAVCEGAVFKKRCIIGYLPQLLEDKYLNRKLGEYFTLFHVTDAKN
                     AGQYARLLTELGLEVGKEDYARLLSTFSGGEKIKIQLLSILLSSPDVLLLDEPTNDID
                     IDTLVWLETFINTCRIPVMYVSHDEMLIENTANVILHMEMHKRKSECSHTISRLSYSE
                     YIKVRKYNLEKQDQIASMQRAEYAKQMERWKKIHDKVEKDQENISRQDPESGRLLKKK
                     MKSVKSQEKRFMREKEDFTEFSDAEEAIFLRFYDGQYINTSKRVLDMELDYLKIGDIV
                     LSADVKLYVKGGEHIGIYGKNGAGKTTLLRQILNHIVQDNNIRLGYMPQDYTDSMDNS
                     LTCIEFLAPSGTKADITKARQYMGGMKFTADEMIGPISGLSGGQKAKLYLLKIVLDGC
                     NVLVMDEPTRNLSPLSNPVIREQLKGFKGTIIAVSHDRKFLNEVCDKRYELTDKGLFM
                     TDMTW"
     gene            complement(2393..>2998)
                     /locus_tag="PKJ65_04405"
     CDS             complement(2393..>2998)
                     /locus_tag="PKJ65_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ41109.1"
                     /translation="SITFGWNSSALVDVATYLPSWDKLTALSIQKRYGYEKVIEEDQD
                     FSGIGNIRYVNTAVSGTSITWGLQNVQELVCNYRPDMVFLAFGMNAGGLDAATFKERL
                     NKIIYEIRKDNSDCSVVLVATTYPNPNLTRKTFYIQEYVHVLHQVKEELGNIAVSDMS
                     TLHKKLLDVKPYHHMTGNNINHPNDFLMRQYAIVTLHALKQ"
BASE COUNT          866 a          671 c          473 g          988 t
ORIGIN      
        1 tctcttcctg agtataaaac ccgccatacg gcttaggatt ataatacctt ccgaaatttg
       61 aatacggtct ttgcgaacct ttttcggtaa gaagaggata tttctttatc tccatacgcc
      121 atccctggtc ttccgtaaga tgccagtgga atttattaaa cttaaaaagc gcagcggcat
      181 caatcatctt cttcagctct tccacggtaa agaaatgcct gcagcaatca attagaaaac
      241 ctctgtggga aaaagcaggt ttatcatgaa ttgtaaggta aggtatattg ccggaataac
      301 cgaattccag ttgtcttaaa gtctgatgtg catagttctc tcccgctttc gtggagcact
      361 taattgttgc tcccttagca tcaattacaa tagagtattc ctcggctgaa tattccgaat
      421 catattcaac cgtaatgctt tccaatatgt ttcttctaaa gaaaagtttg taatcagtgt
      481 gaaactcatt aggcatgggg accagattaa tgctcatgct aaacctccga aattaactgt
      541 atatatattc tatcacaaaa taataagaga ttattagtct gatagtataa aagcaaattt
      601 ttcaaaaaaa acatacacgc ttaccatgtc atatctgtca tgaaaagtcc tttatccgtc
      661 agttcatacc gcttatcgca cacttcgttt agaaactttc tgtcatggga aacggcgatt
      721 atggtacctt taaaaccttt cagttgttca cgtatcacgg gattggataa cggacttaaa
      781 ttccttgtag gttcgtccat cacaagcaca ttacatccat ccagtactat ttttaaaaga
      841 taaagttttg ctttctgtcc gccggacaag ccggaaatcg gaccaatcat ttcatcagcg
      901 gtgaatttca tacctcccat atactgtctt gctttagtaa tatcggcttt tgttccgctc
      961 ggggcaagaa actcaataca tgtaagacta ttatccatac tgtcggtata gtcctgcggc
     1021 atatagccta aacggatatt attgtcctgt acaatatgat taagtatctg tctcaataaa
     1081 gtagtcttac ctgctccgtt ctttccgtat atacctatat gctccccacc tttaacatac
     1141 aatttcacat cggctgaaag tactatatct cctatcttca gataatccaa ttccatatcc
     1201 agtacccttt ttgatgtatt tatatattgt ccgtcataaa atcttaagaa aatcgcttct
     1261 tcggcatcgg aaaactccgt aaaatcttct ttttcacgca tgaatctttt ttcctgtgat
     1321 ttaaccgact tcattttctt cttcagcaat cttccgcttt cgggatcctg tctggatatg
     1381 ttctcctgat ctttctccac tttatcatga atcttcttcc aacgctccat ctgttttgca
     1441 tattccgctc tttgcatgga ggcaatctga tcctgttttt ccagattata tttcctcact
     1501 ttaatatatt cggagtaact taagcgtgat attgtgtggc tgcattcact ttttcttttg
     1561 tgcatttcca tatgtaagat aacattagcc gtgttttcaa taagcatctc atcatgggac
     1621 acatacatta ccggaattct acaggtgttt ataaaggtct ccagccatac aagagtatct
     1681 atatcaatat cattggtagg ttcatccagt agcagcacat ccggcgatga tagcagtata
     1741 gataaaagct gaatctttat cttttctcct ccggagaagg tagaaagcaa ccttgcataa
     1801 tcctctttcc ccacttccag ccccaattcc gtcaacaatc ttgcatattg tccggcattt
     1861 tttgcatcgg ttacatgaaa taaggtaaaa tattctccca atttcctgtt cagatattta
     1921 tcttcaagca actgtggcaa ataccctata atacaccgtt ttttaaaaac agcaccttca
     1981 cagactgcat aatcagaaat actttcgaca tcataaattg ctctaagcaa agtactcttg
     2041 ccgttgcctt cctcgcctat tacagccact ttgtcattag agttcaaatt gatggtaagc
     2101 ctttctacca aaggtctgcc gtcattcttc atatatatgg ataaatcttt aatctgcagc
     2161 ataaaatccc ccaaaaaaat ccgtatgtca tggaatgaaa acggatgctg caataaaaca
     2221 tatttgacag ttataatcct tattctttcc aagacataat aaatacgggt ttcccctgtt
     2281 atctgttatg ttttgtctcg aattaaagaa tcatttcctg cctcttttcc tgaatattat
     2341 ctaaaaaata tcattaaaac agcatattgt caatcagtca actcccaagt ttttattgtt
     2401 tcagcgcatg caatgtcact attgcatact gacgcatcag aaaatcattc ggatgattta
     2461 tattgttgcc cgtcatgtgg tgatagggtt taacatctaa aagtttcttg tgcaaagtgg
     2521 acatgtccga taccgcaata ttacccaact cctctttaac ctgatgcaaa acatgtacat
     2581 actcctgtat gtaaaatgtt tttcgtgtga gattgggatt tgggtaagtt gttgcgacta
     2641 aaacaacgga acaatcggaa ttatcttttc ttatctcgta aataatctta ttaagtctct
     2701 ccttaaaagt agcagcatcc aatccgccgg cattcatacc gaaggctaaa aacaccatgt
     2761 caggtctgta attgcatacc aattcctgca cattctgcag tccccatgta atagatgttc
     2821 ccgaaaccgc agtgtttaca tacctgatat ttcctatacc ggaaaaatcc tgatcctctt
     2881 ctataacctt ctcataaccg tatctctttt gtatacttaa agcggtcagt ttatcccacg
     2941 agggaagata cgtagccaca tccaccaaag cggaactgtt ccagccgaaa gttatgct
//
LOCUS       DAOPWC010000166         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34261_length_2980_cov_2.824957, whole genome shotgun sequence.
ACCESSION   DAOPWC010000166 DAOPWC010000000
VERSION     DAOPWC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34261_length_2980_cov_2.824957"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKJ65_04410"
     CDS             <1..817
                     /locus_tag="PKJ65_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ41110.1"
                     /translation="MVDDFPVTVYGATIHCWAKQYPHGKETFLQAVYNSCNPVFVKVS
                     HLIGIKRFYEYVESFGFRDPSGIELMGEGQSIFHSEPTKLDMSVASFGQRFQITPIQM
                     VAAYNAIANGGYLIKPSLTDAIADKQGNIIYDFEPQVVRQVISEETSDEMRKILQGVV
                     SEGGGKNAYVDGYRIAGKTGTSETTVEGVYVSSFIGFAPADNPVITVLLIVFNPQGDS
                     YYGSQVAAPYAKLIFEQALEELSVEKQGTAGKEKTPVYVPNLNGYNEKKPLRK"
     gene            877..1149
                     /locus_tag="PKJ65_04415"
     CDS             877..1149
                     /locus_tag="PKJ65_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ41111.1"
                     /translation="MYQFPESGTVIAKDGVIILYTYVPENKKTVKMPYLAGKDKSYVV
                     KALKDLGLNINSDGDGECIGVQYPAGTYVEKGTIVNITFRFTDYPD"
     gene            1170..2663
                     /locus_tag="PKJ65_04420"
     CDS             1170..2663
                     /locus_tag="PKJ65_04420"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ41112.1"
                     /translation="MLLKDLLKVTGCIRISGSKNTDITDISFDSRKTKPCHLFIAIDG
                     VDDDGHKYIGNAVSNGAVAIVVTKDFEYYADKYPDVSVIETDDVRIFLAKASAYYFNY
                     PANALNLIGITGTKGKSTISYMIKNIFDKARTACAVIGTVGAVYKDKEIAISQTTPSA
                     YELNEIFSKINKESVNNCVMEVSSIALKQNRVYGLRYKIGMYTNLTQAHITDREHPDF
                     SDYYNSKKRMFSASDIMIVNNDDKYVRKAAKEYKSSEGYNKDKHKIYTIGIEEKADIT
                     ADNILYRNDGCSFIYRGLGMELEIELDMPGRFNIYNSLFAVTACLLNDIDVKYIRQGL
                     SDVVVKGRCEKVDTGKDFTVMIDYAHTPDSLEKLLIAVRETRTSGRIICLFGCGGDRD
                     KSMRPMMGKIAGQNADFSIITSDNSRTEKPEDILFMIEQGIKETDGEYICITDRTEAI
                     YYALSNAKKDDLIILAGKGHETYMDVMGKKTHYDEREIIQKVLKGEI"
     gene            2660..>2980
                     /locus_tag="PKJ65_04425"
     CDS             2660..>2980
                     /locus_tag="PKJ65_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNZ41113.1"
                     /translation="MRIQLGKIYDLFAAGLKADKKTVVTGVSIDSRTVKPGEIFFAIK
                     GENFDGHDFVNKALEQKAVAAVCSKKDKYAGDNIIYVEDTVKALQDLARFYRQSLEIP
                     FIAVT"
BASE COUNT          974 a          513 c          727 g          766 t
ORIGIN      
        1 gatggtggat gattttcccg ttacggttta cggagctact attcactgtt gggcaaagca
       61 atatcctcac ggcaaagaga cattcttaca agccgtatac aattcctgca atccggtttt
      121 tgtaaaagta tctcacctta taggtataaa gaggttttat gagtatgtag aatcattcgg
      181 attcagagat ccttcgggaa tagagcttat gggagaaggg cagagcatat ttcacagtga
      241 acccacaaaa ctggatatgt cggttgcttc cttcggtcag cgatttcaga taacacccat
      301 acagatggta gcggcatata acgcgattgc aaacgggggt tatcttatta aacccagtct
      361 taccgatgct atagcggaca agcaggggaa tataatttac gactttgagc cacaggtagt
      421 gcgacaggtc atatccgaag aaacatccga tgaaatgaga aagattctgc aaggagtggt
      481 atcagagggc ggaggaaaga atgcttatgt tgacggttac aggatagccg gtaagacggg
      541 aaccagtgaa acgacggttg agggcgtgta tgtgtcttct tttatcggat ttgctcctgc
      601 ggataatcct gttataacgg tgttgcttat tgtattcaac ccacagggtg attcctacta
      661 cggcagtcag gttgccgctc cttatgcaaa acttatcttc gagcaggcat tggaagaatt
      721 gtccgtagaa aagcaaggaa cggcgggtaa ggagaaaact ccggtatacg ttcccaattt
      781 aaacgggtat aatgaaaaaa agccgctaag aaaataaaga gttacggctt aaattatgag
      841 attgaaggca gtacggcagg tgaagatgtc actattatgt atcaatttcc cgaaagcggt
      901 accgttatag ccaaagacgg ggttataata ttatatacct acgttcccga aaacaagaag
      961 acggtcaaga tgccgtatct ggcaggtaag gataaatcgt atgttgttaa agcattgaag
     1021 gatttgggat tgaacatcaa ttccgacgga gacggagagt gtataggcgt tcagtatccg
     1081 gccggaacat atgtggaaaa gggcactatt gtgaatataa ctttccggtt taccgattac
     1141 ccggattaag aaaggatgat tgagatagaa tgcttctgaa agatttattg aaagtaacgg
     1201 gttgtataag gatttcaggc tcaaaaaata cggatattac cgacatatcc tttgattcca
     1261 gaaaaacaaa gccgtgtcat ctgtttattg ccatagacgg ggtagacgat gacgggcata
     1321 aatatatagg taatgccgtt tcgaacggtg cggttgccat agttgtaacc aaagattttg
     1381 aatattatgc ggataagtat cccgatgtat cggttattga gactgatgat gtcagaatat
     1441 ttttagcaaa agcttctgca tattacttca attatcccgc aaatgctctc aatcttatag
     1501 gaataaccgg aaccaaaggc aaatccacca tttcctacat gattaagaac atatttgaca
     1561 aagcaaggac ggcatgtgcg gttataggaa ccgtgggagc ggtctataaa gacaaggaaa
     1621 tagccatatc gcagacaacg ccttcggcat atgaactgaa tgagattttc agtaagataa
     1681 ataaagaaag tgttaacaat tgtgtaatgg aagtttcttc catagcgttg aaacaaaaca
     1741 gggtatacgg attacggtat aagataggca tgtataccaa ccttactcag gcacatatta
     1801 ccgacaggga acatccggat ttttccgact actataactc caagaaacgg atgttttcgg
     1861 caagtgacat aatgattgta aataatgatg ataaatatgt aagaaaagct gcaaaagagt
     1921 acaaaagcag tgaaggttat aacaaggata agcataagat atatacgata ggcatagaag
     1981 agaaagccga tataactgcg gacaatatac tgtaccggaa tgacggctgc agttttattt
     2041 accggggttt aggtatggag ctggaaatag agttggatat gccgggaaga tttaatatat
     2101 ataacagcct gtttgccgtt accgcatgtc ttctcaacga tattgatgta aaatatatca
     2161 gacaagggct ttccgatgta gttgtcaaag gaagatgcga aaaagtcgat acgggtaaag
     2221 atttcaccgt aatgattgat tatgctcaca cgcctgacag tctggaaaaa ctgctcattg
     2281 cggtgcggga aaccaggaca tccggtcgga taatatgcct gttcggatgc ggcggtgaca
     2341 gggataagtc catgagaccc atgatgggaa agattgcggg gcaaaatgcc gacttttcca
     2401 taataacatc ggataactcc agaaccgaga agccggaaga tatcctgttc atgattgaac
     2461 agggtattaa agagaccgac ggcgaatata tatgtataac cgacagaacg gaggctattt
     2521 attatgcgct tagtaatgca aaaaaggatg accttataat acttgccggc aaaggacatg
     2581 aaacttatat ggatgttatg ggtaaaaaga cacattatga tgaaagagaa ataatacaaa
     2641 aggtgctgaa aggggaaata tgagaataca actggggaag atatatgatt tgtttgctgc
     2701 cgggttgaaa gccgacaaaa agaccgttgt taccggtgtg tccatagact cgagaaccgt
     2761 aaaacccggg gagattttct ttgctataaa gggcgagaat tttgacggtc atgattttgt
     2821 caataaggct ttggaacaaa aagccgtagc ggccgtctgt tcgaagaaag ataaatatgc
     2881 cggggacaac ataatctatg ttgaagatac cgttaaggca ttgcaggatc tggcaagatt
     2941 ttacagacag tctttggaaa taccctttat cgccgttacg
//
LOCUS       DAOPWC010000167         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34342_length_2975_cov_3.513699, whole genome shotgun sequence.
ACCESSION   DAOPWC010000167 DAOPWC010000000
VERSION     DAOPWC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34342_length_2975_cov_3.513699"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="nrdD"
                     /locus_tag="PKJ65_04430"
     CDS             <1..426
                     /gene="nrdD"
                     /locus_tag="PKJ65_04430"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNZ41114.1"
                     /translation="EGITDKEYYTNSFHIPVSYKISAYDKLQKEAPYHALTNAGHISY
                     VEIDGDPLKNLEAFEDIIRYMKECNIGYGSINHPVDRDSICGYTGVIQNSVCPSCKRE
                     EKNGPKFERIRRITGYLVGTLDNFNDSKAAEERDRVKHA"
     gene            419..937
                     /gene="nrdG"
                     /locus_tag="PKJ65_04435"
     CDS             419..937
                     /gene="nrdG"
                     /locus_tag="PKJ65_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNZ41115.1"
                     /translation="MHKIRLSGKCTKDSTVDGPGLRIVIWTQGCLHNCKGCHNPDTHD
                     LNGGFSEDIDNVIKFIEESKIQKGITLSGGEPFLQPEALIPIVKSAIDKGLNVWAFSG
                     YTYEQLLKDKNKKALLDYVDVLVDGPFVLEQKDERLAYKGSKNQRIIDVKQSYAQNKV
                     VISRYDEVNADF"
     gene            1018..1716
                     /locus_tag="PKJ65_04440"
     CDS             1018..1716
                     /locus_tag="PKJ65_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ41116.1"
                     /translation="MYNILICDDDYDIVSALKIYLSSDNYNIFTAYNGKKAIEIIKEH
                     KIHLVLLDIMMPEVDGITAMVEIRKISNVPIILLTAKSEDNDKILGLNLGADDYITKP
                     FNPVEVLARVKSQLRRYMHLGSQTKSQAVIRIGDIELDDDAKTVTVSGEEVSLTPIEF
                     DILRLLMKNPGKVYSTKQIYSEVWDDEPYGAENTVAVHVRHIREKIEINPAEPRYFKV
                     VWGQGYKMERRNNQ"
     gene            1713..>2975
                     /locus_tag="PKJ65_04445"
     CDS             1713..>2975
                     /locus_tag="PKJ65_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41117.1"
                     /translation="MKNIKYSLSMKIMAYILITISAFIFAVSVSASVYIKVSDIDRNP
                     ETAFFESNICKEITHDYAKEVYYGYFKEYMLTYMDWQKSEDYKQYYSKENTNFFFAIT
                     DNWGNNVLSSYTDTYGYRNTFVMEQNGYTVTAFVRKDITAEDDYLFPYQAYNFAYEIR
                     QFLIPIASISLIVTILLFIYLMYSAAHKKGSDVLEQSFIDKIPLGPLLLLCAGVMVSA
                     HFVLREVAIALYEEFMFEELIMLLSFGFAFAFISLMILISSIAARIKLKTLFRNTLIY
                     IITKAVLGFIRKILANISILWKLLLAVTALTIIELIIIVFFTARGGVVPVVIWLLLRA
                     GMIVLFSFVAVNMAEIKKGGEEISRGNFQYKIDNKHMMTDFKRHADNLNNISEGMSDA
                     INEKMKSERFKSDLITNVSHDIKTPLTSI"
BASE COUNT         1020 a          504 c          639 g          812 t
ORIGIN      
        1 gaaggaataa ccgacaaaga gtactacacc aattccttcc acataccggt atcctataaa
       61 atatccgctt atgacaaact gcaaaaggaa gcaccttacc acgcacttac caatgctggt
      121 catatatctt atgtggaaat agacggagat cctttgaaga atctggaagc tttcgaggat
      181 attatacgat atatgaaaga gtgtaatata ggatacggaa gcataaatca tccggtagac
      241 agagactcga tatgcggtta tacgggtgtt atacaaaatt ccgtttgccc cagctgcaaa
      301 agagaagaga agaacggtcc caagttcgaa agaatacgca ggataacggg ataccttgtc
      361 ggaacactgg ataatttcaa tgattcaaaa gccgcagagg aaagagacag ggtcaaacat
      421 gcataagata agactgtccg gtaagtgcac aaaggacagt actgtggacg gacccggttt
      481 aagaatagtc atttggacac agggatgttt acataactgc aaaggatgtc ataatcccga
      541 cactcacgat ctgaacggcg ggttttcgga agatatagat aatgtaatta aatttataga
      601 ggagtctaaa atacaaaaag gcatcaccct ttcggggggt gagccttttt tgcaaccgga
      661 agctttgata cccatcgtaa aatcggcaat agataaaggt ctcaatgtgt gggcattttc
      721 cggttacaca tacgagcagt tgctgaagga taaaaacaaa aaagctcttc tcgactatgt
      781 cgatgtcctg gtagacggcc cttttgtgct ggagcagaaa gacgagcggc ttgcctacaa
      841 aggatccaaa aaccagagaa taatcgatgt taaacaatct tatgcccaaa acaaggttgt
      901 tataagccga tatgacgagg taaatgcgga tttttagaat cttaataaaa tattaatgat
      961 attaaggctt tttccttaaa catttatagg gcaaaatagt aagtgaagga gacagccatg
     1021 tacaacatac ttatatgcga tgacgattat gatatagttt ccgcattaaa gatttatctt
     1081 tcaagtgata actataatat ttttacggct tataacggaa aaaaggcaat agagattata
     1141 aaagagcaca agatacatct ggtgcttttg gatataatga tgccggaagt ggacggtatc
     1201 accgcaatgg tggagataag aaagataagt aatgtcccca ttattctttt gactgcaaaa
     1261 agtgaagaca atgacaaaat actgggtctg aatttaggag cggatgacta tataaccaag
     1321 cctttcaatc ctgtagaagt attagccagg gtaaaatcgc aactgagaag atatatgcac
     1381 ttaggttcac aaaccaagtc ccaagccgta ataaggatag gagatatcga attggatgat
     1441 gatgccaaga cggtaacggt atcgggagaa gaggtaagtc ttactccgat agagtttgat
     1501 atattaagac tgctcatgaa aaaccccgga aaagtatatt ccactaaaca gatttattcg
     1561 gaagtatggg atgatgaacc gtacggtgcg gaaaataccg ttgcagtaca tgtaagacac
     1621 ataagagaaa agatagaaat aaaccctgcg gaaccgagat atttcaaagt agtctggggg
     1681 cagggttata agatggaaag gcggaacaat caatgaagaa cattaaatac agtttatcaa
     1741 tgaaaattat ggcatacatt cttatcacga taagcgcatt tatcttcgcg gtttcggttt
     1801 ccgcttctgt ttatatcaaa gtaagtgata tagacagaaa tcccgaaaca gctttcttcg
     1861 agagcaatat atgcaaagag ataacccatg attatgcaaa ggaagtctat tacgggtatt
     1921 ttaaagagta catgttgaca tatatggact ggcagaaatc agaagattat aagcagtatt
     1981 attcaaaaga aaatacaaat ttcttttttg caataaccga taactgggga aacaatgtgc
     2041 tttccagtta tacggacaca tacggttaca ggaatacttt tgtcatggag cagaacggtt
     2101 ataccgtaac ggcatttgtg agaaaggata taacggcaga ggatgattat ctgtttccgt
     2161 atcaagcata caattttgca tatgagatac gacaatttct gataccgata gcatcaatat
     2221 ctttaattgt aactattctt ttgtttatat accttatgta ttcggccgct cataaaaaag
     2281 gcagcgatgt tcttgaacag tcctttattg ataaaattcc gctcggtccg ttgcttcttc
     2341 tgtgtgcggg agttatggta tcggctcatt ttgtactcag agaagtggca atagctttgt
     2401 atgaggaatt tatgtttgaa gaactgataa tgcttttatc tttcggattt gcattcgctt
     2461 ttatatcgct catgatactg atatcaagta ttgccgccag gataaaactt aagacactgt
     2521 tcaggaacac actcatatac attatcacaa aagcggtatt gggatttata cggaagatac
     2581 tggccaatat atctattttg tggaaactgc ttctggcagt tacagcatta acaattattg
     2641 aacttataat cattgttttt ttcactgcaa gaggcggtgt tgtaccggta gttatctggc
     2701 ttcttttaag agcaggtatg atagtactgt tctcttttgt cgccgttaac atggcagaaa
     2761 taaagaaagg cggagaagag atatcccggg ggaatttcca atacaagata gacaacaaac
     2821 atatgatgac agattttaag agacatgcgg ataatcttaa caacatttcc gaaggaatgt
     2881 ccgatgcaat aaatgagaaa atgaaatcgg aacgttttaa aagtgatctc ataaccaatg
     2941 tgtcgcatga tataaagaca ccgctgacat ccata
//
LOCUS       DAOPWC010000168         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34457_length_2969_cov_2.525738, whole genome shotgun sequence.
ACCESSION   DAOPWC010000168 DAOPWC010000000
VERSION     DAOPWC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34457_length_2969_cov_2.525738"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKJ65_04450"
     CDS             <1..237
                     /locus_tag="PKJ65_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41118.1"
                     /translation="LRDIFLSSVFVLFTLFVFIAAISEHTGFTYYPVTDSVNIKDFGI
                     VLFITMISVIYLPFITEGIAYVKRGFIKLWLQKR"
     gene            219..1811
                     /locus_tag="PKJ65_04455"
     CDS             219..1811
                     /locus_tag="PKJ65_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41119.1"
                     /translation="MASKALISIKNMGFTYENYAMGKTVNKVALTDINLDIYEGEILL
                     IMGPSGCGKTTLLRSLKKEAVPYGISTGKVESATDSSKISIVFSNPETQLVTTTVLSD
                     LALTMENLGYDAYTQKKRMSETVSYFGIEDILHRHPDTLSGGQKQIVSLCSQLMVHPE
                     ILLLDEPISQLDPISALEFTDILRRLNEEIGITIVMTEHRPDDVISLANRVVYMEDSR
                     ILFQGQSREVLKKLWETGKERVRDFLPVVSKCSLLIDKERPACITSKELNFMVRDYLP
                     IKKAQKENDDISFKMSEEKTVRIRELFFAYTKEKYAIRNLDLDVCKGEFLCIMGSNGS
                     GKTTLLKIMSGILKPHIGRIKASFGKIQYMPQELNLFFTEDTVYEQMSFKEMDKDLYL
                     SLVEELNLKDILYKHPYDLSSGEQQKAVLACMLLRKPDFILLDEPTKSMDPSLKRVIS
                     RLLKQSGATIVCATHDLEFAAKYADRCVMMFNGEIAYSMDARNFFKENRYYTTSVNRA
                     FGRIDTDMVLYEDVENAFGGTV"
     gene            1808..2497
                     /locus_tag="PKJ65_04460"
     CDS             1808..2497
                     /locus_tag="PKJ65_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ41120.1"
                     /translation="MKRHWYFFIISFVLVIASLILSYSSMALALILAIMGILLMGFNV
                     FERKEVSAEKIVLLAILTAVSAAGRVLFSSLPSVNPSSFIIIMSAMIFGPSFGFMTGA
                     LTALVSNMLLGHGPWTLWQMLFWGLMGFLAGVLKKPLKEHMWFRLAYGFVWGFLFGWG
                     MNVFYVLSGYIAEPGIKTFLVACSASFVLDLLHAGTNLLLILLLGNGFIKIFERAAAK
                     YGLKDVPIKRE"
     gene            2586..>2969
                     /locus_tag="PKJ65_04465"
     CDS             2586..>2969
                     /locus_tag="PKJ65_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ41121.1"
                     /translation="METIILHADLNSFYASVECLYNPAIRNLPVAVCGSVENRHGIVL
                     AKNQQAKAVGIRTGEAVWQAKLKAANLVVVPPHYDRYLRFGNLAREIYCSYSEKVQPF
                     GMDEAWIDISFPGRTMDQGYEIAEKI"
BASE COUNT          863 a          524 c          708 g          874 t
ORIGIN      
        1 ttaagagata tttttctgtc gtcggtattt gttcttttta ctttatttgt gttcatagcg
       61 gcaatttcgg agcatacagg atttacctat tatcccgtta ccgattccgt taatattaag
      121 gatttcggca ttgtactgtt tattaccatg atatcggtta tttatctgcc tttcataaca
      181 gaaggtattg catatgtaaa gagaggattt attaagttat ggcttcaaaa gcgctgataa
      241 gcattaaaaa tatgggattc acatatgaaa actatgccat gggtaagacg gttaataagg
      301 tagctcttac cgatattaac ctggatatat atgaaggcga aatacttctt atcatggggc
      361 cttccggttg cggaaagacc actctgcttc gaagtctgaa aaaagaagcc gttccttacg
      421 gaatttccac aggtaaagtg gaaagcgcga ccgacagcag taaaatctcc atagtgtttt
      481 ccaatcccga aactcaactg gtcaccacaa cggtgttaag cgatctggca ttgacgatgg
      541 agaacctagg ctatgatgcc tatactcaga agaagcgaat gagtgaaacg gtaagctatt
      601 tcggtataga agatattttg cacaggcatc ccgacacatt atccggcggg cagaagcaga
      661 tagtatctct ttgctcacag ttaatggtgc atccggaaat attgcttctt gatgaaccca
      721 taagtcagct ggatcccatc tccgctctgg agtttaccga tattttgcgt agattgaatg
      781 aagagatcgg tattaccata gttatgaccg aacacagacc cgacgatgta atatcgttgg
      841 caaacagggt tgtatatatg gaagattccc gtatactgtt tcagggacaa tcgcgtgagg
      901 tgcttaaaaa actttgggaa accggcaaag agagagtgag agatttttta cccgtagttt
      961 caaagtgctc gctgctcatt gataaagaga gaccggcctg cataacatca aaagaattga
     1021 attttatggt gcgggattat ttaccgataa agaaggctca aaaggagaat gatgatattt
     1081 cctttaaaat gtctgaagaa aaaaccgtca ggataagaga gttatttttc gcatatacaa
     1141 aggaaaagta tgctataagg aatctggatc tggatgtatg taaaggggag ttcttatgta
     1201 taatgggtag caacggctcg ggaaaaacca cccttcttaa gattatgtct ggaatactga
     1261 aacctcatat cggaaggata aaggcatcat tcggaaaaat acagtatatg cctcaggagt
     1321 tgaatttgtt ttttaccgaa gacacggtgt atgaacagat gtcttttaag gaaatggaca
     1381 aggacttata cctgtccctg gtagaggaat tgaacttaaa agacatactg tataagcatc
     1441 cgtatgacct ttcctcggga gaacagcaga aagcggtgct ggcatgtatg ctgttgagaa
     1501 agccggattt tatacttctg gatgagccga caaagagcat ggatccttct ttaaaacgcg
     1561 taatatcgcg gttattaaaa caatcgggag caacaatagt gtgtgccaca catgatttgg
     1621 aatttgccgc aaaatatgcc gaccgctgtg taatgatgtt taatggtgag atagcatact
     1681 ccatggatgc aagaaacttt tttaaagaaa acagatatta taccacctcg gtcaacagag
     1741 ccttcggcag gattgatacc gatatggtgc tgtacgagga tgtggaaaat gcattcggag
     1801 gaacagtatg aaaagacatt ggtacttttt cataatttcc tttgttcttg ttattgcttc
     1861 tttgatactg tcttacagca gtatggcatt ggccctgata cttgccatta tgggtatact
     1921 gctgatggga ttcaatgttt tcgaaagaaa agaagtaagt gccgagaaga tagtattgct
     1981 ggccatactt accgccgtat cggcagccgg gagggtgctt ttctcctctt tacccagtgt
     2041 taatccgtct tcttttataa tcataatgtc ggcaatgata ttcggtcctt ctttcggttt
     2101 tatgaccggt gctttgacag ctctggtctc caatatgttg ctcggacacg gaccgtggac
     2161 attatggcag atgctttttt ggggactgat gggctttttg gcgggagtgc tgaaaaaacc
     2221 gttaaaagag catatgtggt tcagacttgc ctacggtttt gtatggggat ttttattcgg
     2281 ttggggaatg aatgtttttt atgtgctgtc gggatatatt gccgaacccg gaataaagac
     2341 ttttctggtt gcctgttccg cttcctttgt gcttgattta ttgcatgcag gtaccaatct
     2401 tcttctgata ctgcttttgg gtaacgggtt tataaagata tttgaaagag cggcagctaa
     2461 atacggtctt aaggatgttc ctattaagag agaataaata tatgggaaga atatcggaca
     2521 gttaaaagcg aacactttac aaacatatgt tcgtattata taatacgcct gaaggtaggg
     2581 gtattatgga aactattatt ttgcatgcgg acttaaacag tttttatgct tcggtggagt
     2641 gtctgtataa tcctgccata agaaatctgc cggttgccgt atgcggaagc gtggagaaca
     2701 ggcacggaat agtacttgcc aaaaatcaac aggcaaaggc tgtaggtata agaacgggag
     2761 aagcggtgtg gcaggcaaag cttaaagccg ccaatcttgt agtggtgcct ccgcattatg
     2821 acagatatct tcggttcggc aatcttgcaa gagagattta ctgttcctat tccgaaaagg
     2881 ttcagccttt cggtatggac gaggcctgga tagatatttc ttttccgggc agaacaatgg
     2941 atcaaggtta cgagatagcc gagaagata
//
LOCUS       DAOPWC010000169         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34512_length_2965_cov_3.357732, whole genome shotgun sequence.
ACCESSION   DAOPWC010000169 DAOPWC010000000
VERSION     DAOPWC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34512_length_2965_cov_3.357732"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PKJ65_04470"
     CDS             <1..1486
                     /locus_tag="PKJ65_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNZ41122.1"
                     /translation="VDEADTVKTDGKFVYSISSYGFVNIVAVNSDGYLELIKQIKDFG
                     KNFSPMEIYVDDQFLIVIGTSYDKEGSYSAAYIYDKTDIAELRLLKDVMIKGYYSDSR
                     KIGNSLYLICNYTPYYYYLKDTDLLPCYVVNGKTTYQSPSDIFYFDETECSSYLILGA
                     VDLSDLTKDINLNTYLGSGQTVYMSHENLYVTYTQYPYRSVRTIFPEENSDYKEETVI
                     HKFRADNAVLNYVATAKITGYPINQFAMDEHKGFFRIAVTDNMEGKSVNNVYVFDENL
                     VQTGTIQNMAPGERIYSVRFNGDIGYMVTFVVVDPLFVMDLSDPYNPKVDGELKIPGY
                     STYMHMYSDTLILGFGMDTTEQDGRALNQGIKVAMFDVSDRANPKQLFTTSIGGRGTW
                     SELNYNHKAFMFDREKDIFAFPASISGNNYEMKTQGLVVCKIDMRNNRFEVLDILSQD
                     YFTNWSNYVERGMFIGNTLLTISKNAIYSYNYTTLQFIDNVLFD"
     gene            complement(1461..2738)
                     /locus_tag="PKJ65_04475"
     CDS             complement(1461..2738)
                     /locus_tag="PKJ65_04475"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HNZ41123.1"
                     /translation="MEQYLEDMIEDLRGLIAIKSVKDEPAPNAPYGEGTLKALEYMLN
                     LGKKYGMKAVNIDGRAGYLEYGEGKHMIGVLLHLDVVPEQDGWESCPYTLTERDGFLY
                     GRGVSDDKGPAIAAFYALVDLIKKGEIKGYRVRLIFGLDEECGSSCMAHYVKTEELPT
                     MGFVPDADFPVIYAEKGILTVAIGGEGNKEISLQAGDRANVVAGKCRVEYEEKEFVYE
                     GKPAHGAAPFNGINAIKIAIENNPVLAGIPIIEFFRDALADKHYGEGLDIECKDETGR
                     LTVNPGVMIIDKEKSVCVLNIRYPVTYDFEDITERIERVCGKYDLKVIHTDNNPPLFV
                     DKNSVLVSTLLSVYREMTNDFTEPIAIGGGSYARSMPNLVAFGMNMPGDPESAHQANE
                     CIKKQRLHEGAAIYRESIKRLGETLINQKGHCQ"
BASE COUNT          865 a          679 c          538 g          883 t
ORIGIN      
        1 agtggatgaa gccgataccg taaagaccga cggaaaattt gtatattcca tcagtagtta
       61 cggttttgta aatattgtcg ctgtcaattc tgacggttat ctggagctga taaagcagat
      121 aaaggatttc ggaaaaaact tcagtcccat ggagatatat gttgatgacc agttccttat
      181 agtaatcgga acatcctacg acaaggaagg ttcttactct gcagcataca tatatgacaa
      241 aaccgatata gcggaactca gattacttaa ggatgtaatg ataaagggtt actactccga
      301 ttccagaaag ataggcaact ctctttattt aatctgcaac tatacccctt attactacta
      361 tttaaaggat acggaccttc tgccctgcta tgttgtaaac ggtaaaacca cataccagtc
      421 accttcggat atcttctatt ttgacgagac cgaatgttca tcatacctta tactgggtgc
      481 tgttgattta tccgatctta ccaaggatat taaccttaat acttacctgg gaagcggtca
      541 gaccgtgtat atgtcccatg aaaatctgta tgttacctac acacagtatc cttacaggtc
      601 tgtcagaacg atattccccg aagagaactc ggattataag gaagaaaccg taatacacaa
      661 attccgtgcc gataatgccg tattgaatta tgttgccaca gccaagataa cgggatatcc
      721 catcaatcag tttgcaatgg atgaacacaa aggatttttc agaatagcgg ttaccgataa
      781 tatggaaggc aaatcggtta ataatgtata tgtctttgat gaaaatctgg tgcagacagg
      841 tacaattcaa aacatggcac ccggagaaag gatatattcc gtaagattta acggagacat
      901 cggttatatg gttacattcg ttgtggttga ccctctcttt gtaatggatc tatccgaccc
      961 gtacaatcct aaagtagacg gagaactcaa gatccccgga tattcaacct acatgcacat
     1021 gtattccgac acactcatac tgggcttcgg aatggataca acggaacagg acggcagagc
     1081 tttgaatcag ggtataaaag ttgcaatgtt tgatgtttcg gacagagcca atcccaaaca
     1141 gcttttcaca acttccatcg gcggtcgcgg cacatggtcg gaactgaatt acaaccataa
     1201 ggcctttatg tttgaccgtg aaaaagacat attcgcattc cccgcttcaa taagcggcaa
     1261 caactatgaa atgaagacac aaggattggt tgtatgtaaa atcgatatgc gaaacaaccg
     1321 gtttgaagta ctggatatac tgtctcagga ttactttacc aactggtcca actatgtgga
     1381 aagaggtatg tttataggaa acaccttatt aacaatctct aaaaatgcca tctattcgta
     1441 caactacact acattacagt ttattgacaa tgtccttttt gattgataag cgtttctcct
     1501 aatcttttta tactctctct gtaaatagcg gcgccttcat gcaggcgctg cttttttata
     1561 cactcatttg cctgatgagc actttcggga tcaccgggca tgttcatacc gaatgccaca
     1621 agattgggca tacttcttgc gtaggaacct cctcctatcg ctataggttc ggtaaagtca
     1681 tttgtcattt ccctgtacac cgaaagcaat gttgatacca gtaccgagtt tttatcgaca
     1741 aacagcggag ggttattatc cgtatgaatc actttcaaat catatttccc gcatactctc
     1801 tctattctct ccgttatatc ttcaaaatca taagttacgg gatagcgtat attaagtacg
     1861 cagacggatt tttccttgtc tataatcata actccgggat ttaccgtaag tcttcctgtc
     1921 tcatctttac attcaatatc cagtccttct ccgtaatgct tatcggcaag tgcatctctg
     1981 aaaaactcaa taatcggtat accggcaaga accggattgt tttcaatggc tatcttaatt
     2041 gcgtttatgc cgttaaaagg agccgctccg tgagcaggtt taccttcata aacaaactct
     2101 ttttcttcat actccacacg acattttccc gctaccacat tggctcgatc ccccgcttgt
     2161 aacgatattt ccttgttccc ctctcctcct atagccaccg taagaattcc tttttcggca
     2221 taaattacgg ggaaatccgc atccggtaca aatcccattg tcggaagttc ctccgtcttt
     2281 acataatgag ccatacagga agagccgcat tcctcatcca aaccgaatat aaggcgaacc
     2341 ctgtaccctt ttatctcacc ttttttaata aggtcaacaa gagcataaaa tgcggcaatt
     2401 gccggtcctt tatcgtccga cactcctctg ccgtataaga aaccgtctct ttcggtcaat
     2461 gtatacgggc agctttccca accgtcctgt tcgggaacca catccagatg aagaagcaca
     2521 cctatcatgt gttttccttc accgtattca agatatcccg ctcttccgtc aatatttaca
     2581 gccttcatac cgtatttctt gccgagattc agcatatact ccaaagcttt gagtgtacct
     2641 tctccgtacg gtgcattagg agcaggctca tctttaacgc tttttatggc tatcagccct
     2701 cttaaatctt caatcatatc ctcaagatat tgttccattt ataacttttc tccttaaagc
     2761 agtaaaatgc agtatcattt tcaagtacat attatatcag atctgcggta taaataatcg
     2821 agtagggcag aggcaaaatg aaaccgttcc tacaaaccta taaaataccg catataggaa
     2881 aaaacctatg cacaatattt ctgttgtatt cctgcttcta ccttgttggt tacaatctga
     2941 tttatcgcta aatccaattg cagag
//
LOCUS       DAOPWC010000170         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34759_length_2949_cov_2.410850, whole genome shotgun sequence.
ACCESSION   DAOPWC010000170 DAOPWC010000000
VERSION     DAOPWC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34759_length_2949_cov_2.410850"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKJ65_04480"
     CDS             <1..365
                     /locus_tag="PKJ65_04480"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase"
                     /protein_id="HNZ41124.1"
                     /translation="DEYLGFGLSAHSLYNETRYSNTEDLHQYISLLNKYEKPVVHTDK
                     LTKEEIITEYIILKLRMNSGLDIAHFQDKFDLNFEEKYGDKLKMLVNTGLLYKQSDSY
                     KLTRSGMDVANKVFVEFV"
     gene            489..1355
                     /locus_tag="PKJ65_04485"
     CDS             489..1355
                     /locus_tag="PKJ65_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNZ41125.1"
                     /translation="MHITITGMLGSGKSTICRIISEQTGYEVYSTGKIQRQVAAQKGI
                     STLELNKMMTDNTELDNIIDNETVRISDAVKDRIILFDSRMAWFFVKRSYKVFLTVDP
                     LVAAQRVAKADRGGVEDYTDVNEAKQKLLERAAAEHERFIKIYGADYWDYSNYDLVLD
                     ATWHDPHTLAGIITGQFAMFTESDEQQSAILMSPRSLYPAYIAGKRKKQIDKKCWNAA
                     NVRIVADNHYNYVIKGHSVVLRALENRTEFITVLYNKDIKSSELTVASDSLSLFENSG
                     QFNYPSLPDNVI"
     gene            1465..2490
                     /gene="hrcA"
                     /locus_tag="PKJ65_04490"
     CDS             1465..2490
                     /gene="hrcA"
                     /locus_tag="PKJ65_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNZ41126.1"
                     /translation="MDKRAADILLVIIEEYVNSAEPVGSKSITEKYPLGVSSATVRNE
                     MHKLEEEGYITSLHTSSGRIPTDKGYRFYVDYLTERIKQREIEEKIYKLLKRVEIEET
                     LDDILERLFLGNPYTSLGVLKRNVSRIDTFHILSVAKDTYLVLVIFRNGIAAHRLIKE
                     QLSDKVQVNILSEYLNEKLYGITLRDIQTTDFAFIDTFDRDNKAFFIRVVSEVMDILH
                     QYEDNEFFLKGKENLLSFPDFKELSEVKSIFDAFSREMELSELLLCPTDDAFSVSIGN
                     EIKNENFNKLSIAKHTYDLGAEGKITFAVAGPTRMDYRKVMNSLNYMETILGDIIYDK
                     NDKKEVQ"
     gene            2490..>2949
                     /locus_tag="PKJ65_04495"
     CDS             2490..>2949
                     /locus_tag="PKJ65_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ41127.1"
                     /translation="MAKKKQDNDVNIQQDGADVNNTGNEEVTSCETDMEKQNKELTDC
                     YKRLAAEYDNYRKRTAKEKQDIYDICVADITEKFLPVLDNLDLALANCDSADDNVVKG
                     IELIHKQFLETLAKLDVFEIDCLNKPFDPNIHDAVMVTEDPAIETGTVVEV"
BASE COUNT         1002 a          508 c          660 g          779 t
ORIGIN      
        1 gtgacgaata cttaggattc ggtttatccg cacattcttt gtataatgag acccgataca
       61 gtaataccga ggatttacat caatacatat ctttattgaa taaatatgaa aagcccgttg
      121 tacacaccga taaacttaca aaagaggaga ttataaccga gtacattatt cttaaactaa
      181 gaatgaacag cggattggat attgctcatt tccaagacaa attcgacctg aattttgagg
      241 aaaagtacgg tgataagtta aaaatgcttg taaatacagg cttgttatat aagcaatcgg
      301 acagttataa actgacaagg tcgggaatgg atgtggcaaa taaggtattt gtggagtttg
      361 tgtaaatatt tgaggaaata gaggaataaa acatcattta cacttgcccg tattgaaccg
      421 actttggtat atttatgtag atacaaggca ctaagccata tataatatgt aatcgggagc
      481 gcaaacttat gcatattact ataacgggga tgctgggaag cggtaaatcc accatatgca
      541 gaatcatatc ggaacagaca ggttatgagg tatattccac aggaaaaata caaagacagg
      601 tagctgcaca aaaaggtatc tccactttgg aacttaataa gatgatgacg gataataccg
      661 aactggacaa tattatagac aatgagaccg tccgtatctc cgatgcggtt aaagacagga
      721 taatactgtt cgattcccgt atggcatggt tctttgttaa gcggtcatat aaagtatttc
      781 ttaccgtaga tcctcttgta gcggctcaac gtgtagccaa agccgacaga gggggagtgg
      841 aagattatac cgatgtaaat gaagcaaaac aaaaactttt ggaaagagct gcagccgaac
      901 atgaacgttt tatcaaaatc tacggtgccg attactggga ttattccaat tacgatcttg
      961 tactggatgc cacatggcat gatccccaca ctttagccgg tattataacg ggacaattcg
     1021 ccatgtttac ggaaagcgat gagcagcaat ccgccatact tatgtctccc agatcgcttt
     1081 atcccgcata tattgcaggg aagagaaaga agcaaattga taagaaatgc tggaatgccg
     1141 caaatgtacg gatagttgcc gataatcact acaattatgt tattaaaggt cattccgtag
     1201 tgttaagagc attggagaac aggacggagt ttattacggt tctgtacaat aaggatatta
     1261 aatcttccga attgacggtt gcttccgatt cattaagtct ttttgagaat tccggtcaat
     1321 ttaactaccc cagtctgccg gataacgtga tttaatcaca tatctcttct ttttaacaaa
     1381 atagatgttg acaaaaatat gacaggatgg tatctttgag tagagttagc actctgcggt
     1441 gttgagtgct aaaaaaggag aaaaatggat aaaagagcag ccgacatact gttggttata
     1501 atcgaagaat atgttaacag cgcggagccg gtaggatcaa aaagtattac cgaaaagtat
     1561 cctctgggtg tatccagcgc taccgtaagg aatgaaatgc acaaactcga agaagagggg
     1621 tacattactt ccttgcacac ttcttcgggt cgtataccta cagataaagg ttatcgcttt
     1681 tatgtggatt atcttaccga gcggataaag caaagagaga tagaggaaaa gatatacaag
     1741 ctgcttaaga gagtggaaat agaagaaaca ctggacgata tactggaaag actcttttta
     1801 ggtaatccgt atacttcttt gggagtatta aagagaaatg taagccgtat agatacattc
     1861 catatacttt cggtagccaa agatacttat ctcgtactgg ttatcttcag aaacgggata
     1921 gcggcacaca ggcttattaa agagcagctc agtgacaaag tacaggtaaa cattttatcc
     1981 gagtatctca acgaaaagct gtacggtata acattgcgag acattcagac aaccgatttc
     2041 gcctttatag atacattcga ccgcgacaac aaagctttct ttatccgggt ggtatcggaa
     2101 gtaatggata tactgcatca gtatgaggac aacgagttct tcctcaaagg aaaggaaaac
     2161 cttttgtctt tcccggattt caaggaactg tcggaagtaa agagtatctt tgatgctttc
     2221 agcagggaaa tggaactttc cgaactgctg ttatgtccta ccgacgatgc tttttccgtc
     2281 agtataggaa atgaaattaa aaatgagaat ttcaacaaac tttccatagc caaacataca
     2341 tatgatctgg gggcggaagg gaaaataacc tttgcggtcg cgggaccgac aagaatggat
     2401 tacaggaaag taatgaactc tctcaattat atggaaacca tattgggaga cattatatac
     2461 gacaaaaatg acaagaagga ggtacagtag tggcaaagaa gaagcaagat aatgatgtga
     2521 atatacagca ggacggagcg gatgttaata ataccggtaa tgaagaagta acttcgtgtg
     2581 aaaccgatat ggaaaaacag aacaaagaac tgaccgattg ctataaacgt cttgcagcgg
     2641 aatatgataa ttacaggaaa agaaccgcca aagaaaagca ggacatctat gatatatgtg
     2701 ttgcggacat tacggaaaaa ttcttacctg tattggataa ccttgatttg gcattggcaa
     2761 actgcgattc ggccgatgac aatgtcgtaa aggggataga actgatacat aagcaattcc
     2821 tggaaaccct ggcaaaactg gatgtttttg agattgattg tcttaataag ccgtttgatc
     2881 ccaatattca cgatgcggta atggtaacgg aagatccggc tattgagacg ggaaccgttg
     2941 tagaggttt
//
LOCUS       DAOPWC010000171         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34788_length_2947_cov_2.865491, whole genome shotgun sequence.
ACCESSION   DAOPWC010000171 DAOPWC010000000
VERSION     DAOPWC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34788_length_2947_cov_2.865491"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /gene="recJ"
                     /locus_tag="PKJ65_04500"
     CDS             complement(<1..1509)
                     /gene="recJ"
                     /locus_tag="PKJ65_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNZ41128.1"
                     /translation="MMFNNQWNLSGIVDISNTNLAQSILKSRNIEDLNTYLYPKLSDL
                     HDPYLLKDMDKAVARILQSVKNKELILIYGDYDTDGITATAIMYRFLKSIGAKVTYFI
                     PERNDGYGLSEDVLKRLFNNEKYRETGLVITVDCGIPSVKEVDFINEIGIDCIISDHH
                     TVQEEIPDAYCIISPKVSENYLFRELSGAGIAFKIIQALSVKLNKQEEFAKYIDLAAL
                     GTIADQVPLIDENRTIAKIGMDSIPYTQNTGLKELINQSYPNGIDKVNSSDLIFNIIP
                     KINASGRMESAEHAIKLLISDDYEQCKILSEKLIELNDERKILQKEITDKVMDKIESD
                     ESIKSNAVIVLYDEDWEIGILGIVASKIVDKYNRPCMLLSGSEKYFGKEKLLKGTGRS
                     VEGFNLIEALIHCHSFLDKEAETCGGHYFAAGNVLKESSVDKFSAIINQYAKDIGFTV
                     SDRKLLCPDYKIDIFDINVDNARILSCLEPHGPENELSLFYIEDMILEKSRLI"
     gene            complement(1506..2522)
                     /locus_tag="PKJ65_04505"
     CDS             complement(1506..2522)
                     /locus_tag="PKJ65_04505"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNZ41129.1"
                     /translation="MKSGSHLKILLIVVILLSLMLLNSCGVVKFPVYVYGLIDTEGNY
                     LVQPEFTGIKIGKEGLYPVRQGFNEYYKWGFCDRNGNIVIEPQYKDASMFSEGLAPVQ
                     VYDNSGLWIFIDKENNEAFEKQFKYALPFSEGLACVQSAARDASYNLWGYIDKTGEYV
                     IEPQFQIAASFSEGLAKIQTGSGSAGLCGYIDKTGEIIIEPQFSYAGLFSGGLASVKL
                     SMDQETNDAGYIDRQGNIVIPLIYYNTSTFSDGLAPVLAGDTFKDALYGYINIRNEMV
                     INPKYYMAYSFSEGLAAVKTDQYENSGWMYIDTEGKLVIKNDYSSASSFIDGVAPVGM
                     VEYR"
     gene            complement(2527..>2947)
                     /locus_tag="PKJ65_04510"
     CDS             complement(2527..>2947)
                     /locus_tag="PKJ65_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HNZ41130.1"
                     /translation="KHHPFTSPMDEDIPLLHTQPEKVRAKAYDIVLNGEEIGGGSIRI
                     HDSNFQEKMLEVLGFTKDKAQESFGFLLNALKYGAPPHGGMAYGLDRLVMSITKTDNI
                     RDVIAFPKIQSGACPLTDAPNIVEKKQLDELGICLKE"
BASE COUNT          812 a          653 c          484 g          998 t
ORIGIN      
        1 tatcaatcgt gatttttcca gtatcatatc ttctatataa aaaagactta attcattttc
       61 cggaccgtga ggttccagac aggaaagaat ccgtgcatta tccacattta tatcaaatat
      121 atctatcttg taatcaggac agagcaattt acggtcagaa acagtaaatc ctatatcttt
      181 tgcatactga ttaataatcg cggaaaattt atccacactt gattctttta atacgttacc
      241 tgcagcgaaa taatgaccgc cgcaagtttc cgcttcttta tctaaaaaag aatgacagtg
      301 tatcagtgct tcaataagat taaaaccttc tacggaacgt ccggtgcctt ttaacagttt
      361 ttctttgccg aagtactttt cactgccgct cagcaacatg cacggtctgt tgtatttatc
      421 aacaatcttg gaagcgacta ttccgagtat tcctatctcc caatcttcat catagagaac
      481 aataaccgca ttacttttta tactttcatc gctttctatc ttgtccataa ccttatcggt
      541 tatttctttt tgcagtattt ttctctcatc attcagctca atcaactttt ccgacagtat
      601 tttacattgc tcataatcat ccgatataag cagtttaatt gcatgctccg ccgattccat
      661 tcttccggat gcattgattt taggtattat gttaaagata agatcggaag aattcacttt
      721 atcaattccg ttggggtaag attgatttat aagttccttc agacctgtat tctgagtata
      781 cggaatactg tccattccta tcttggcgat agttctgttt tcatcaataa gcggtacctg
      841 atcggctatg gtccctaatg ccgctaagtc aatatacttt gcaaattctt cctgtttatt
      901 cagtttaacg gataaagcct ggataatttt aaatgcaatc cccgctccgc tcaattctcg
      961 gaacagatag ttttctgaaa ccttcggaga aataatacaa taagcatcgg gaatttcttc
     1021 ctgaacggta tgatgatcgg aaataatgca gtctattcct atctcattga taaaatccac
     1081 ttccttaacc gaaggaatac cgcagtccac agttataaca agacctgttt ctctatactt
     1141 ttcattatta aatagtcttt taagaacatc ttcagataaa ccgtatccgt catttctttc
     1201 cggtatgaag tatgttacct ttgcaccgat gctcttcaga aatctgtaca taatcgctgt
     1261 tgccgtaata ccgtcggtat catagtcgcc gtaaatcagt atcagttctt tgttcttcac
     1321 cgactgcaag atacgggcta ccgctttatc catatcctta agcaaataag gatcatgtaa
     1381 atcactcagt ttaggataaa gataggtatt caggtcttct atattacgac tctttaatat
     1441 tgactgtgct aaatttgtgt tgcttatatc aactattcct gaaagattcc attgattatt
     1501 aaacatcatc tgtattctac cattccgaca ggagctactc cgtctataaa tgacgatgcc
     1561 gaactgtaat cattcttaat aacaagttta ccctcggtat ctatatacat ccaaccggag
     1621 ttttcatatt gatcggtttt aacggctgcc agaccctcgg aaaaggaata tgccatgtag
     1681 tatttggggt ttatgaccat ttcatttctt atgttaatat agccgtacag ggcatcttta
     1741 aaggtatcac ccgcaagtac cggagccaat ccgtcactga acgtggaagt attatagtat
     1801 atcaacggaa ttactatatt accctgtctg tctatatatc cggcatcgtt tgtctcctgg
     1861 tccatgctca gttttaccga tgctaaaccg cctgaaaata gacccgcata ggaaaactga
     1921 ggttctatta ttatctcacc tgttttgtct atatatccgc ataaacctgc cgatcccgat
     1981 cctgtctgga tttttgcaag cccttcggaa aaagaagctg caatctgaaa ctgcggttct
     2041 attacatact cccctgtttt atctatgtat ccccatagat tgtatgatgc atctcttgct
     2101 gcagattgaa cacatgcaag cccttcggag aacggtaaag catacttgaa ttgcttttca
     2161 aatgcttcgt tattttcttt atcgataaaa atccatagcc cggaattgtc ataaacctgt
     2221 acaggtgcca gtccttcgct gaacatgctt gcatccttgt actgcggttc aatcacaata
     2281 ttaccgtttc tgtcacagaa accccattta tagtattcgt taaatccttg tcttacagga
     2341 tataagcctt cttttcctat ctttattccg gtaaattccg gttgcacaag atagttacct
     2401 tccgtgtcga taagcccgta aacataaacg ggaaatttta ccacaccgca actgttcaac
     2461 agcatcagac ttaaaagtat tacaactatt aataatatct ttaagtgact tccggatttc
     2521 atcttatcat tccttcaagc atatgcctaa ttcatccaat tgcttctttt ccactatatt
     2581 aggtgcatcg gtaagcggac aggcaccgct ttgaattttc ggaaaagcta taacatcgcg
     2641 tatattgtcg gttttggtta tgctcattac cagtctgtcc agaccgtatg ccattccgcc
     2701 gtgaggaggt gccccgtatt ttagagcatt cagaagaaaa ccgaaagatt cttgcgcttt
     2761 atcttttgta aatcctaaaa cttccagcat cttctcctgg aaatttgagt catgtattct
     2821 tatacttcct ccgcctatct cttcaccgtt taatactata tcgtatgctt ttgctctgac
     2881 tttttccggt tgtgtatgca ataagggtat atcttcatcc atgggtgatg tgaacgggtg
     2941 atgcttt
//
LOCUS       DAOPWC010000172         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_34804_length_2946_cov_3.187132, whole genome shotgun sequence.
ACCESSION   DAOPWC010000172 DAOPWC010000000
VERSION     DAOPWC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34804_length_2946_cov_3.187132"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /gene="dxs"
                     /locus_tag="PKJ65_04515"
     CDS             complement(<1..1310)
                     /gene="dxs"
                     /locus_tag="PKJ65_04515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNZ41131.1"
                     /translation="MELLDRIKVSSDVKDIKKEDIDELCRQIRTFLVEVVTGNGGHLA
                     SNLGVVELTVALHRCLDMEKDHIIFDVGHQSYVHKILTGRKDSLATMGSIDGLGCFPD
                     EEESRYDSFNTGHASTSVSAALGMEKALRMSGSDGKVVAVLGDGALGGGMVYEALNNA
                     KDLDSDILIILNDNEMSISRNVGAMSRHLSKLKLRPSYIETNVRIKRIVEKTPGGKGF
                     IRLIQKTKRALKSFVGQKMFFEDLDIRYLGPVDGHDEHQLESIIKEALNVKGPKLLHV
                     VTTKGKGYEKAERDPVKFHGLKNSYSKSRRLPENIMYNQLFGKLLTEMAEKDKNIVCV
                     CPAMTEGSGLSEFCKVHKDRYFDVGICEQHAVTFSAGLARDGIKPVCVVYSSFLQRAY
                     DQILHDIGLTNKNVVLCVDRAGVVDAYGKTHQGIYDIAYLSTIP"
     gene            complement(1314..2183)
                     /locus_tag="PKJ65_04520"
     CDS             complement(1314..2183)
                     /locus_tag="PKJ65_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ41132.1"
                     /translation="MTNLNTVMKKYGEQVTLFINQRINKSNAPDIIKEACLYSVNAGG
                     KRIRPVLFLMTLDALNAEICEKHIAYASAIEMIHTYSLIHDDLPAMDNDEYRRGQLTS
                     HIKFGEAYAILAGDALLNLAYETMLDYAVNAKDIAAARNIAEAAGYQGMVGGQVLDME
                     KPDTVNDILAMYSLKTGALIKTSVVSAGIIAGCNKRTLKRLADYAEGIGVAFQLRDDY
                     LDKTDINEKTGKPKDSDKKNEKATYISKRGVAANRNKLQKYTKKALTALQPYGDSYNN
                     LRLLAEFLGDRMK"
     gene            complement(2180..2386)
                     /gene="xseB"
                     /locus_tag="PKJ65_04525"
     CDS             complement(2180..2386)
                     /gene="xseB"
                     /locus_tag="PKJ65_04525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNZ41133.1"
                     /translation="MSNKDFNTLYRELEKKVELLEKGELPLEEAVKTYTEGQELIKLL
                     NEKLNKAREQMVVTDKSEQKEPEK"
     gene            complement(2379..>2946)
                     /locus_tag="PKJ65_04530"
     CDS             complement(2379..>2946)
                     /locus_tag="PKJ65_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ41134.1"
                     /translation="NEEELAYAIHNSNIPVISAIGHETDFTIADFVADARAATPTAAA
                     QMVIPEKKRLERDVLTQKEILEFSYAKLISNLSERVSDLAKRPVMKRPEEYTNILAMN
                     LDNLTGRFFTFSKQYLDNVAYKLSTLIHKADAMNPLAVLSRGYSITSDQKGHVIKSAD
                     NLNKNDVILTTLHAGKIRSVVEKAVKDE"
BASE COUNT          704 a          742 c          501 g          999 t
ORIGIN      
        1 ttgggaattg tggacagata tgcaatatca taaatcccct gatgggtctt accgtatgca
       61 tccacaaccc ccgccctgtc cacacacaac accacatttt tgtttgtcaa ccctatgtca
      121 tgtagtatct gatcgtatgc cctttgtaaa aaagatgagt atacaacaca taccggcttt
      181 attccgtctc tggcaagacc tgcactgaaa gtaaccgcat gctgttcgca tatacctaca
      241 tcaaaatatc tgtccttatg tactttacaa aactcgctta aacccgatcc ttcggtcatt
      301 gcgggacata cacaaactat attcttatct ttttcggcca tttcggtcag aagttttccg
      361 aaaagttgat tatacataat attttcaggc agtcttctgc tttttgaata actgttttta
      421 agaccgtgaa acttaacagg atctctttcc gccttttcat accccttacc tttggtagtt
      481 accacatgca gcagtttagg cccctttaca ttcaatgcct cctttattat agactccagc
      541 tgatgctcgt catgtccgtc gacgggaccc agatatcgga tgtccaaatc ctcgaaaaac
      601 atcttctgcc ctacaaaaga ctttaatgct ctttttgttt tttgtatcaa tcgtataaat
      661 cccttgccgc ccggtgtttt ttccacaata cgcttaattc ttacattggt ctcaatatat
      721 gaaggacgta acttgagttt tgaaagatgt ctagacattg cccccacatt cctgcttatg
      781 gacatttcat tgtcgttaag aattatgaga atatcggaat ccaaatcttt agcgttgttc
      841 aaggcctcgt ataccattcc gccgcccaaa gctccgtcac ctaaaaccgc cactactttg
      901 ccgtccgaac cgctcattct taaggctttt tccataccta aagcggcgga gaccgatgtg
      961 cttgcatgtc cggtattgaa agaatcgtac ctgctttcct cctcatcggg aaagcaaccc
     1021 aatccgtcga tgcttcccat tgttgccaga ctatcttttc ttccggtcag aattttatga
     1081 acataacttt gatgccccac atcgaaaatt atgtggtctt tttccatatc cagacatctg
     1141 tgcagcgcca ccgtcagttc caccactccc agattacttg caaggtgtcc tccgttaccg
     1201 gtcacaacct ccacaagaaa agtccttatc tgtctgcata actcatctat atcctctttt
     1261 ttgatatcct tcacatcgga agataccttt attctgtcca gaagttccat acactatttc
     1321 atcctatcac ctaaaaactc cgctaataag cgtaagttat tatagctgtc gccgtaaggc
     1381 tgtaaagccg ttaatgcctt tttcgtatac ttttgcagtt tattccggtt tgccgcaacc
     1441 cctcttttgg agatgtatgt ggctttttca ttttttttgt cactgtcttt gggttttcct
     1501 gttttttcgt taatatcggt tttatccaga taatcatctc ttaactggaa agctacaccg
     1561 ataccttcgg cataatcagc cagtcttttc aatgttcttt tgttgcatcc ggctattatt
     1621 ccggctgata ccaccgatgt ttttataagg gcacccgttt tcaacgaata catagcaaga
     1681 atgtcattaa cggtatcggg cttttccata tccagaacct gtccccctac catcccctgg
     1741 tatccggccg cttcggctat gtttcttgca gctgcaatat cctttgcatt gactgcataa
     1801 tccagcattg tttcatatgc caggttcaat aaagcgtctc cggctaaaat agcgtatgcc
     1861 tcaccgaatt tgatatgact ggtcaactgc cctcttctgt actcatcatt atccattgcc
     1921 ggcaaatcgt cgtgtatcaa cgaatatgtg tgtatcatct caatagccga tgcatatgct
     1981 atatgctttt cgcaaatttc cgcattgaga gcatccaagg tcattagaaa cagaaccggt
     2041 cggattcttt taccgccagc atttacactg tacaggcagg cttcctttat tatgtcgggc
     2101 gcattgcttt tgtttatcct ctgattgata aacagagtaa cctgttcccc gtattttttc
     2161 attacggtat tcaagtttgt catttttccg gctccttttg ttcggattta tcggtcacta
     2221 ccatttgttc acgggctttg ttaagttttt cgttgagcag ttttatcaac tcctgccctt
     2281 cggtataggt ctttaccgct tcttccaacg gcaattcccc tttttccagt aactccacct
     2341 tcttttccag ttccctgtag agcgtattga aatccttatt actcatcttt taccgccttt
     2401 tccaccacac tccttatctt tcccgcatga agagtggtta atataacatc atttttattc
     2461 agattgtccg cactttttat aacatgtcct ttttgatcgc tcgttatact gtatcccctc
     2521 gataacaccg ctaaaggatt catggcatcc gctttatgta ttaatgtact taatttatat
     2581 gcaacattgt ccagatattg ctttgagaag gtgaaaaacc tgcctgtaag gttatccaga
     2641 ttcatagcca atatattggt atattcctcc ggtcgcttca taaccggtct ttttgccaaa
     2701 tcggaaactc tttcagaaag gttacttatt agttttgcat aggaaaattc cagaatttcc
     2761 ttttgcgtaa gcacatctct ttccagacgt tttttctccg ggataaccat ctgagcggct
     2821 gccgtaggtg ttgcggctct ggcatccgcc acaaagtccg caattgtgaa atccgtttca
     2881 tgacctattg cggatatgac gggaatgttg ctgttatgta ttgcatacgc caactcttct
     2941 tcgttg
//
LOCUS       DAOPWC010000173         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_35299_length_2914_cov_2.723330, whole genome shotgun sequence.
ACCESSION   DAOPWC010000173 DAOPWC010000000
VERSION     DAOPWC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35299_length_2914_cov_2.723330"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKJ65_04535"
     CDS             complement(<1..223)
                     /locus_tag="PKJ65_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HNZ41135.1"
                     /translation="MSEIIKNKDGFIIDMDGVLYHGNKLLEGALEFIDWLQTNGKKYL
                     FLTNSSERSPLELSQKLARMGIVVGEEHFY"
     gene            complement(228..1520)
                     /locus_tag="PKJ65_04540"
     CDS             complement(228..1520)
                     /locus_tag="PKJ65_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ41136.1"
                     /translation="MKYQLVLAGGGARGSYQIGVWKALRELNIEIDMVIGTSVGSING
                     AAFVCGAFEQAEYLWKTLDMPTLFDITYDKEDGEPGSVEYIESILKNKGLDTSTLKSY
                     LEQVIDEEVLRNSPVDYALVTFSLTKMKPVILFKKDIPEGKVIDFILASSAVPGFKKQ
                     EIDGQLYIDGGIYDNLPVNVLIEHGYKNIIAVSNAAIGVYRRVQQSDAKILYIENQQI
                     EGGILEFDPELAARNMLLGYLDGMKAFGKFIGKNYYISDNATHDYAKPLTDREIEMLY
                     LNVKKSKRFVTESVLQFSLLRTLRNNTDGVLTAKRVFFAGMEITANTFGIEKIRAYTP
                     EQLIAKIMAAYRSAKSAHRQIMKEKNNFFKNLIDKIWDKNRIRFDGKAAKKLYLGQVL
                     IDKDNPSISLITTTEHLIANLFIYLMLWRRKKIKKDRR"
     gene            complement(1577..2347)
                     /locus_tag="PKJ65_04545"
     CDS             complement(1577..2347)
                     /locus_tag="PKJ65_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41137.1"
                     /translation="MLKRKTHTEGRITAESCICCFVLIFVLLLSIQLNGYIKAHSDLL
                     SELDRRLINGAVAYHASKIYPVDIITRPDATDIDNAIFFAVPYDDFFTLSVFADYSGI
                     LKKNRVYVRSVSSKWAGDGKEVVKENIWELDPLERGQVIHKMMGANLDHNFPTLDIYD
                     GYTKEAVSIVSINTQEDSYKSGTELKRKIKKHIVSIDKFTYGEYNGYSVSGEDIREKT
                     VLVVIPNAKLNDHQTRQINDMLKYAKKVGINLEIKKYR"
     gene            complement(2322..>2914)
                     /locus_tag="PKJ65_04550"
     CDS             complement(2322..>2914)
                     /locus_tag="PKJ65_04550"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HNZ41138.1"
                     /translation="GENRYLDGEIEYIIFGNRDNRKNIQSCKASLMALRFACNAIHVY
                     TDLPKTTKADALAACLAGSWTFGAAAPIAKNLILCSWAIAESAYDTEALCRGAKCPVI
                     KMDGDWNLDIGLEKGLSKTPDWLKIDYDDYLRLLLCTKSNNVKVLRLLDLIALNSPDN
                     MDMSSVCCGIKVGVTLKFKGLLGTERSFYVEAEDSY"
BASE COUNT          767 a          689 c          505 g          953 t
ORIGIN      
        1 tatagaaatg ctcttctccg acaacaatac ccatccttgc aagtttctgt gacagttcca
       61 gcggcgaacg ttcggaactg ttggttaaga atagatactt tttgccgttg gtttgcaacc
      121 agtcaataaa ttccaacgct ccttccagta atttattccc atgatacaag acaccgtcca
      181 tatctattat gaaaccgtct ttattcttaa ttatttcgct cattttttta tctcctgtct
      241 ttcttaattt tcttacgtct ccacagcatc aggtatatga aaaggttggc tataagatgc
      301 tcggtagtcg taatcaggct gatggaagga ttatccttat caatcagcac ctgtcctaaa
      361 taaagctttt ttgcagcctt tccgtcaaac cttattctgt ttttatccca tattttatct
      421 atcagattct taaagaagtt attcttttct ttcataatct gtctgtgagc cgatttggcg
      481 gaacggtatg cagccattat ctttgcaatg agctgttccg gagtgtaagc ccttattttc
      541 tctatgccga atgtgtttgc ggtaatctcc attcccgcaa aaaatactct ttttgcggta
      601 agcacaccgt cggtattgtt tcttaaagtt cttaatagag aaaactgcag caccgattcc
      661 gtaacaaatc ttttgctttt cttaacattc agataaagca tttcaatttc tctgtcggtc
      721 aatggttttg catagtcatg agttgcattg tcggatatat agtaattctt tccgataaac
      781 tttccgaacg ctttcattcc gtcgagatat cccagcagca tattgcgggc tgctaactcg
      841 gggtcaaact ccaatatccc tccttcaatc tgctgattct ctatgtataa tatcttcgca
      901 tccgattgct gaactcttct gtatacacct atagctgcat tgctgaccgc tattatattt
      961 ttatagccgt gctcaataag aacattaaca ggcaaattgt cgtatatgcc gccgtcaata
     1021 tacaactggc cgtctatctc ctgcttttta aatccgggta cggcggaact tgcaagtata
     1081 aagtctatta ccttaccctc cggtatatct ttcttaaaca gtatgacggg tttcattttc
     1141 gtcaaggaaa atgtcaccaa agcataatcc acgggtgaat tgcgcaagac ctcttcatct
     1201 attacctgtt ccagataact tttcaatgtg gaggtatcca gacctttgtt cttaagtatg
     1261 gattcgatat actcgacaga acccggctct ccgtcttctt tgtcataggt aatatcaaag
     1321 agagtgggca tatccaatgt cttccataag tattcggcct gctcaaaagc accgcagaca
     1381 aaagccgctc cgtttataga gcccacggaa gtgcctatga ccatgtcaat ctctatattc
     1441 agttctctca gagctttcca tacacctatc tggtaggaac ctctggctcc gcctccggca
     1501 agcacaagct gatatttcaa gtccattacc tacccggatt gattatatat aacctgtaac
     1561 ttttttacaa ttgtaactac cgatatttct ttatttcgag atttataccc actttttttg
     1621 catatttaag catatcgttt atctgtctgg tctgatggtc attaagcttg gcattgggta
     1681 ttaccaccaa cacggttttt tcccttatat cttccccgct gaccgaataa ccgttgtatt
     1741 ctccgtaggt aaacttatct atggagacaa tatgtttttt tatttttctc ttcagttcgg
     1801 taccggactt atagctgtct tcctgcgtat ttatacttac gatagatacc gcctcttttg
     1861 tataaccgtc atatatatcc aaagtcggaa aattatgatc cagattggct cccatcattt
     1921 tatgtattac ctgacctcgt tctaaagggt ccaattccca gatattctct ttaacaacct
     1981 ctttaccgtc tcccgcccat ttagatgata ccgaacgaac ataaactctg tttttcttca
     2041 atatcccgct gtaatcggca aagaccgaga gagtaaagaa gtcatcatac ggaaccgcaa
     2101 aaaatattgc attgtctata tcggtcgcat caggtcgggt aattatatcc acaggatata
     2161 tttttgatgc atggtatgcg acggctccat tgataagtcg cctgtccagt tcggacaaca
     2221 gatcggaatg tgcctttata tacccgttca gttgtatgct caacaacagt acaaatatga
     2281 gtacaaagca acatatacag ctttccgccg ttattctgcc ctcagtatga gtcttccgct
     2341 tcaacataaa aactcctttc ggtacccaaa agacctttga actttaaagt tactcccacc
     2401 ttgatgccgc agcacaccga agacatgtcc atattgtccg gagaattgag agcaatcaga
     2461 tccagcagcc ttaagacctt gacattattg ctttttgtgc ataaaagaag ccgtaaatag
     2521 tcatcataat caattttcag ccagtcgggt gttttactta aacccttttc cagtcctata
     2581 tccagattcc agtcaccgtc catcttgatt accggacact tcgctcctct gcaaagagcc
     2641 tcggtatcgt atgccgattc cgctatagcc caggaacata agataagatt cttggctatg
     2701 ggtgctgccg caccgaaagt ccagctgcca gcaagacatg ccgccaatgc atcggctttg
     2761 gtagtcttag gtaaatcggt atagacatga atggcgttac aagcaaatct caatgccata
     2821 agagacgctt tgcatgattg gatattcttt ctgttgtctc tgtttccgaa aatgatatat
     2881 tcaatttcgc catctaaata tctgttctca cctt
//
LOCUS       DAOPWC010000174         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_35519_length_2902_cov_3.658939, whole genome shotgun sequence.
ACCESSION   DAOPWC010000174 DAOPWC010000000
VERSION     DAOPWC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35519_length_2902_cov_3.658939"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKJ65_04555"
     CDS             complement(<1..660)
                     /locus_tag="PKJ65_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41139.1"
                     /translation="MVGRINIIIMAISAGAFIILPLVFSVVMIIRKKTKSMLLGMASF
                     SIYGIVVKNLIFSLIFLVPFLKTRLSSDYIVYSLFIGITSALIHMATRYALHRKIGIK
                     DKAAFSAGESVIQCIYVGIPTTIIGLGYAVTINLGGFEYIGITNKVLPALTSLINADC
                     TEYLPYVIYPFFAFALNRMTVDLFIKNKFVSALTEAVILSIYSYFMLCRLYISGTIFL
                     SA"
     gene            complement(664..1443)
                     /locus_tag="PKJ65_04560"
     CDS             complement(664..1443)
                     /locus_tag="PKJ65_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNZ41140.1"
                     /translation="MGASVINCKFSSNLLYYQDECNVILPSLAKPEDLKVLYLLHGMH
                     GTCHDWITYTNIVRLAENYRVAVVCPNAKNSFYADMRYGEPFYTYLTTEFMEYVQRTF
                     GFSAKRKDNIIAGLSMGGYGALKIAFNNPDKFCIAGSFSGVLDMANIKGFVTGDEQTD
                     RKNMQLFISIFGEDPVLLGTDNDLISLVQRNKTVNGYSVKDLLVYQYCGTSDFLSLHN
                     MSFKKCAEEAKLNLKYATDGGSHDWERWDEQIALFLQEYFG"
     gene            complement(1505..2683)
                     /locus_tag="PKJ65_04565"
     CDS             complement(1505..2683)
                     /locus_tag="PKJ65_04565"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNZ41141.1"
                     /translation="MDFYSNLRNKLQAMKDGGTYKEYQYLTTPMSNKSDIEKYDEVIV
                     LCSNNYIGLCNNREVIQAGHEALDKYGAGAASVRFICGTYDIHRQLEKETAEFMHTES
                     CLTYMSCWNANTGVIPAIVFEGDCIISDALNHASIIDGCRLTSRKVERFVYKHSDMED
                     LERLLKEGERFNTRLVITDGVFSMEGDIAKLDEIYKLCKKYKAILLVDDSHGMGVVGA
                     TGRGAIEYHNLLGKIDIITGTYGKALGGSAGGFVAAKKEVIDMCIQASRTSLFTNSLP
                     PVVAAMSLKAIQLLKRDNSIIESLHKKVKYLRQRLHEEGFEPLEGESAIVPIIVGDTA
                     KAIRIAKAMLEKKVYVTGFGFPVVPEGTARIRIQVSDALSYEDIDYSVKAFKEVFDNL
                     "
BASE COUNT          874 a          655 c          505 g          868 t
ORIGIN      
        1 agccgaaaga aatattgtgc cggaaatata taacctgcac agcataaagt acgaatagat
       61 tgataggata acggcttcgg taagcgccga cacaaacttg tttttaataa acaggtcaac
      121 agtcatccgg tttaatgcaa acgcaaaaaa cggatagata acgtacggca gatattcggt
      181 acagtcggca ttaataaggg aggttaaagc gggcagtact ttatttgtta tccctatata
      241 ttcaaaacct ccgagattta ttgtaacggc atatccaaga cctattatcg ttgtgggtat
      301 gcccacatat atgcactgaa tgaccgattc gcctgctgaa aaagctgctt tgtctttaat
      361 acctatcttt ctatgcagag catatcttgt agccatatgt attaaagcac ttgtaatacc
      421 gataaaaaga gaataaacaa tataatcgga agacaaccgt gtcttcaaaa aaggaacgag
      481 gaaaataagg gagaatataa gatttttcac tacaattccg taaatggaaa aactcgccat
      541 acctaaaagc atggactttg tttttttccg tataatcatg acaacagaaa aaacaagggg
      601 cagaattata aatgcccctg cagatattgc catgataata atgttaatac ggcctaccat
      661 aatttatccg aaatactcct gcaaaaacag agctatctgc tcatcccagc gctcccaatc
      721 atgggagccg ccgtcggtag catatttaag gtttaatttc gcttcttccg cacacttttt
      781 gaaactcatg ttgtgaaggg aaagaaaatc cgatgttccg cagtactgat aaaccaaaag
      841 gtcttttacg gaatatccgt taaccgtctt gtttctttgt acaagagaaa taagatcgtt
      901 gtcggttcct aaaagtacgg ggtcttcacc gaaaatgctt ataaacaact gcatattttt
      961 cctgtcggtc tgctcatcgc cggtaacaaa acctttaatg ttcgccatat ccaacactcc
     1021 ggaaaaactt cccgcaatac agaatttgtc gggattatta aatgctattt tcaaagcacc
     1081 gtatcctccc atggataaac cggcaataat attgtctttt ctcttggcgg aaaatccgaa
     1141 tgtgcgttga acatactcca taaactcggt agtaagatat gtgtagaaag gctcaccgta
     1201 tcgcatatcg gcataaaaac tgttttttgc gttggggcag accaccgcaa ctctataatt
     1261 ctcggcaagc cttactatat ttgtatatgt aatccagtca tggcatgtcc cgtgcatacc
     1321 gtgcagaaga tacaatacct ttaaatcttc gggctttgcc aaagacggca gtataacatt
     1381 acattcatcc tgatagtaaa gaagattgga actgaatttg caattgatta ccgaagcacc
     1441 cattttatac ctcacacaat ttatttgagt atactaaaac gtcccaatca ggaacgtctt
     1501 atacttacag attatcaaaa acttccttaa aagctttaac cgaataatcg atatcttcgt
     1561 aagacaacgc gtcggaaacc tgtattctta tccttgcggt accctccggg accacgggaa
     1621 atccgaatcc ggtaacataa accttctttt ccagcatagc tttggctatt ctgatggctt
     1681 ttgcggtatc tcccacaatt ataggcacta ttgcactttc tccttccaaa ggctcgaatc
     1741 cttcttcatg cagtcgctga cgcagatatt ttaccttctt atgtaacgac tctataatgc
     1801 tgttatctct cttaagcaac tgtattgctt ttaacgacat tgccgcaaca accggcggaa
     1861 gactgtttgt gaacagcgat gttctggagg cctggataca catatcgatt acttcctttt
     1921 tagcggccac aaaacctccg gccgaacctc cgagcgcttt accgtatgtt ccggttataa
     1981 tatctatctt gcctaagaga ttgtgatatt ctatggctcc ccttcccgtt gcacctacta
     2041 cacccattcc gtgggaatca tccaccaata agattgcttt gtatttcttg cacagtttat
     2101 atatctcatc cagtttggca atatcgcctt ccatggaaaa cacgccgtcg gttataacca
     2161 gccttgtgtt gaacctttcg ccttctttca gaagccgctc cagatcttcc atgtccgaat
     2221 gcttgtatac gaatctttcc actttcctgc tggttaaacg acatccgtct attatactgg
     2281 catgattcaa cgcgtcggat atgatacagt ctccttcaaa aacaatagcg ggaattacac
     2341 cggtattggc attccagcac gacatatatg ttaaacagct ctcggtatgc ataaactccg
     2401 ccgtttcctt ctcaagttgc ctgtgtatat cataagtacc gcagataaat ctgacggaag
     2461 cggcacctgc accgtattta tccaatgctt catgtcctgc ctgtattact tccctgttat
     2521 tgcaaagccc tatatagtta ttggaacaca atacgataac ctcgtcatat ttttcaatat
     2581 ccgatttatt actcataggt gttgtaagat actgatactc tttgtaagta cctccgtctt
     2641 tcattgcctg aagcttattt cttaaattcg aataaaaatc catgatacac atctccttgt
     2701 catactttaa gtcatatatt aattatacac tcttttcatg caaggtgaac taaaacaatg
     2761 aatatatggt aagcatacca atttaatacc gatagaataa gcttatttaa taaccatgtt
     2821 gctgatatat tgaacgaatg ttacagataa ctgcattaat ctgcaacaat aaccgtcccg
     2881 tcaaccgatg agaaatatct tt
//
LOCUS       DAOPWC010000175         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_35765_length_2887_cov_4.152189, whole genome shotgun sequence.
ACCESSION   DAOPWC010000175 DAOPWC010000000
VERSION     DAOPWC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35765_length_2887_cov_4.152189"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKJ65_04570"
     CDS             complement(<1..325)
                     /locus_tag="PKJ65_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41142.1"
                     /translation="MSTVTKKKSRWIIAAIVLILSVAVLLFFVITTNVTYGSGKSVDT
                     FSTYQDLVEKSPVAVIATVVSDNKEFVYDDIIFAVTEIEVKDCVRGDLGNRTLEILQT
                     KMKEDP"
     gene            518..1840
                     /gene="melA"
                     /locus_tag="PKJ65_04575"
     CDS             518..1840
                     /gene="melA"
                     /locus_tag="PKJ65_04575"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNZ41143.1"
                     /translation="MKKFAFIGGGSFGFTRNLVKDLLTFPAFRDAHIVLMDIDPERLD
                     YIHQAVRKIIKAGKYPAKVTATTDRVKALKGADGVLCTILSGDVDVWQHDILIPKKYG
                     VDINIGDTRGPAGIFRFLRTVPVMLDICKDIEKYCPDAVFLNYTNPMAMLCMAMQKKF
                     PNLKITGLCHSVQGTAGMLAKWIGAPMNEITYKCAGVNHQAFYLEYKWNGKDAYPLIR
                     EAIKDPEIYQKELIRNNMFEHLDYYVTESSGHNSEYVAWYRKNPELLEKYCMNGTNWN
                     PGHYAYILNEYLKRKDTWRDSIKEWLAKEDINLERGYEYATYIFNAVFGDNTLFKFNG
                     NVMNHGIIENLPYDCCVEVPILASKNSLEPIHIGVMPDQLATLINTTARCEVMAVDAA
                     LERNPRKVFHAVAFDPLTSAVLSLDQIKEMVDEMMEKNKDYLGYLYKQ"
     gene            complement(1966..2412)
                     /locus_tag="PKJ65_04580"
     CDS             complement(1966..2412)
                     /locus_tag="PKJ65_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNZ41144.1"
                     /translation="MKKEFKGRNVVAGTVQTTAVVSKNGMNTLATFQKSLLANKKTVL
                     GSDQNNPDLYKKEITGKAICLPKTIGSTTGGMVLQSAAAMGVAPKAMLFSESIDSIAA
                     AGVILADVWTENSIVTVDKLGQDFLDYVKDGMTVTVKQDGTVIVEQ"
     gene            complement(2409..>2887)
                     /locus_tag="PKJ65_04585"
     CDS             complement(2409..>2887)
                     /locus_tag="PKJ65_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HNZ41145.1"
                     /translation="VELKEALIKDKAKVYVIDDAELERVKKNYPVIWKNADAKPSRCF
                     IGCPHLSYNQLIEWTDRIGKELEKQGKKKVALETVFTAAPDVVAHFKENKEYYNKFIS
                     YGTKISSICPLMYMNNPICGKRAVVTNSNKLRTYTTARYYTDEEIAGIAVRGDIAR"
BASE COUNT          799 a          609 c          559 g          920 t
ORIGIN      
        1 aaggatcctc cttcattttt gtttgcagta tttctaatgt tctgttgcct aaatctcctc
       61 ttacacagtc tttaacctcg atctccgtaa cggcaaagat aatgtcatcg tacacaaact
      121 ctttgttatc cgaaacaact gtagctatta cggcaacggg tgatttttcc accaggtcct
      181 gataggtgct gaatgtgtca actgatttcc cgcttccata tgtaacattt gtcgttataa
      241 caaaaaacaa aagaacggca accgataata ttaaaacgat tgccgctatt atccacctgc
      301 tttttttctt agttacggta gacactttat tccctccgtg ataataatcg gactctgctt
      361 tgttcagaat tcagcattct cctctgtccg gaattgctcc atcatgcatc atttgacgaa
      421 tagtataaat ctaaagttct caggtcatta aataaaggaa cattacatac ataaaaatga
      481 tacaatatcc gtatatcatt gtattaagga gtgtgccatg aaaaaatttg catttatagg
      541 cggcggcagt ttcggcttta cacgtaatct ggtgaaggac cttctcactt tccccgcatt
      601 tcgtgatgcc catatcgttc tgatggatat agatcccgaa agattggatt atatacatca
      661 ggctgtcagg aagattataa aagcgggaaa atatcctgca aaagttacag caactacaga
      721 cagggttaaa gctcttaaag gagcggacgg tgtattgtgt acgatacttt ccggtgatgt
      781 ggatgtttgg cagcatgata tcctcattcc caaaaaatac ggagtggata taaatatcgg
      841 agacacaaga ggtcccgcag gtatcttccg ttttttaaga acggtgccgg taatgctgga
      901 catatgtaaa gacattgaaa aatactgtcc cgatgcggtt ttcttgaact acacaaaccc
      961 gatggctatg ttatgtatgg ccatgcagaa gaaattcccc aatcttaaga taaccggctt
     1021 gtgtcacagt gttcagggaa cggcgggaat gcttgcaaaa tggatcggtg ctcccatgaa
     1081 tgaaataact tacaaatgtg cgggtgtaaa ccatcaggct ttctatctgg aatacaagtg
     1141 gaacggaaaa gatgcgtatc ctttaatcag agaagccatt aaagatcctg aaatttacca
     1201 aaaagaactt atccggaaca atatgttcga gcatctggat tattatgtaa cggaatcttc
     1261 cggccacaac agtgaatatg tcgcatggta cagaaagaat cccgaattat tggaaaaata
     1321 ctgtatgaac ggtacaaact ggaaccccgg acattatgca tatattctta atgaatatct
     1381 gaaacggaaa gacacctggc gtgactccat taaggaatgg cttgcaaagg aggatattaa
     1441 tctggagcgt ggctacgagt atgctacata tattttcaat gcggtattcg gagataatac
     1501 attattcaag ttcaacggaa atgtaatgaa tcacggaatt atcgaaaatc ttccgtatga
     1561 ctgctgtgtt gaagttccta ttcttgcatc aaagaattca ttggaaccga tacatatagg
     1621 ggttatgccc gatcagcttg ccacccttat aaacacaacg gcccgctgtg aggtaatggc
     1681 tgtggatgcg gcactggaac gtaacccgag aaaggtgttc catgcggttg cattcgatcc
     1741 gcttacttcc gccgtactgt cgcttgatca gataaaagag atggttgatg aaatgatgga
     1801 gaagaataag gattatctgg gttatttgta taaacaataa taaataggga gttttctctt
     1861 tatcctttta tccaagacaa agggttatgt ctctacataa cccttttttg ttcttcaaat
     1921 actcttctta ctttccttta gctttttcag ttcttctttg ttgtatcatt gctctacaat
     1981 aaccgttccg tcctgcttta ctgtaacggt cattccgtct ttgacataat ccagaaaatc
     2041 ctgtcccagt ttatccacgg ttacaatact gttttctgtc cacacatcgg caagtataac
     2101 tcctgctgcg gctattgaat ctatactttc actgaacagc atagctttcg gagcaactcc
     2161 catagcggcc gcactttgca gaaccattcc cccggtagtg gaacctatgg tcttaggcag
     2221 acatattgct ttgccggtta tctctttttt gtataagtcg ggattgttct gatcgcttcc
     2281 caaaaccgtt ttcttatttg ccaataaact tttctgaaat gtagccaaag tattcatgcc
     2341 gttttttgat accacggctg ttgtttgaac cgttcccgct acaacatttc ttcctttaaa
     2401 ttcttttttc atcttgcaat atcccccctt accgcaatac cggctatttc ttcatcggta
     2461 taatatcttg ccgttgtgta agtcctcagt ttattggagt ttgtcactac ggctcttttt
     2521 ccgcaaatag gattgttcat atacataaga gggcatatgg aagatatttt tgtgccgtaa
     2581 cttataaatt tgttgtagta ttccttgttt tctttgaaat gtgctacaac atcgggagcg
     2641 gcggtaaata ccgtttccag agcaactttt ttcttgccct gtttttccag ctcttttcct
     2701 attctgtcgg tccattcaat cagctgattg tatgacagat gcggacaccc tataaaacac
     2761 cttgaaggct ttgcatcggc attcttccat atgacagggt aatttttctt aaccctttcc
     2821 agctcggcat catctatcac atatactttt gctttgtctt ttatgagtgc ttctttcagt
     2881 tccactg
//
LOCUS       DAOPWC010000176         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36064_length_2871_cov_3.034446, whole genome shotgun sequence.
ACCESSION   DAOPWC010000176 DAOPWC010000000
VERSION     DAOPWC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36064_length_2871_cov_3.034446"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1269)
                     /locus_tag="PKJ65_04590"
     CDS             complement(40..1269)
                     /locus_tag="PKJ65_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41146.1"
                     /translation="MNYIKRHMEEVVLRLSEQFPAILITGPRQVGKTTMLKELMKSEP
                     ENRAYVTLDDISDRMLAKTDPAMFFQLHKPPIFIDEVQYAPELFPYVKILIDRGCNPG
                     DFWMTGSQIFKLMRGVQESLAGRVALLQMLPLSQSEITGGVTVPFTTDFDTLFERQKA
                     VASVSAPELFERIWKGGMPALVSGKYKDRSVVYSGYIKTYLERDVSDLSEAIDAMKFM
                     RFITAVAARTAQLVNYKGIADDAEIDQYTAKNWLNILETLGIIFYLHPYSNNVLKRTI
                     KTPKLYFFDTGLVCYLTKWSSSETAMNGAMSGALLENYTIAEIIKSYQNCGREPFIYY
                     YRDRDSKEIDLIIEGDGKLCPVEIKKAAMPDKRLVKSFSIIDKSPLKRGTGAILCLAE
                     KLGAFDKDNLIVPIWLI"
     gene            complement(1342..>2871)
                     /locus_tag="PKJ65_04595"
     CDS             complement(1342..>2871)
                     /locus_tag="PKJ65_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41147.1"
                     /translation="NFPLERKDIVAKDIAIDMRTENTNYGIDWIRRYVCSKYVNYKIT
                     DCNVSISDSEVHNIRYSFKNRDSHILIAQQYRISVNAANDIEGMTRGEANNYDIMLQA
                     VFVADNINNRFVLSGIIPENEMIDSSKKLYDYVKTDGRFSLGAYVTDTVDVKAIPTLA
                     KYSDRITSYDLKDSKIISELTKDITFEQTHSGPTEKYLPVGKDKVVFFLPEPVSKLYM
                     VNYRTNEIIWSKDYPDTRCIKLDYTDRYSGAADEVIMFYYTKNIEHLYDYIDTMGEYI
                     AKNMPSDAYEKPIPGTKWRAINENYSIHLKDTETDTVYTVLSANIADWPVKDYLFQKA
                     LDENRFVYQKIHSGEGMLYFNLYAYDIRDGSSTAIGRYDRDRGYYIFSNEADEFVTSA
                     AVSGGAGSQTTMYIYDDEKKEAVDIKSLAEDFPYIVDYSLSKDGRYVAILTGESGYTF
                     ELVWAFVADHILLIDLKTKEVLLQDSVTLNGGQIFFLPDNTLAVNDGKMLMYIDVSGI
                     N"
BASE COUNT          796 a          627 c          506 g          942 t
ORIGIN      
        1 atgaagtggc atattttgat tatgaggaat agataatatc taaattagcc atattggaac
       61 tataaggttg tctttgtcaa atgcaccgag tttttcggca agacaaagta tcgcacctgt
      121 tcccctttta agaggtgact tgtcaataat tgaaaaactt ttaaccagcc gcttgtccgg
      181 catggctgcc ttttttattt ccacggggca caattttccg tcaccttcga taataaggtc
      241 aatttcctta ctatcccggt ctcggtagta gtatataaaa ggttcacgcc cgcagttttg
      301 gtaactctta ataatctccg ctattgtata gttttccaac aatgccccgc tcatagcacc
      361 gttcatagct gtttcggagc tggaccattt ggtaagatag cagactaacc ccgtatcgaa
      421 gaaatagagc tttggcgttt ttatagtacg ctttagaaca ttgtttgaat acggatgtaa
      481 atagaaaatt ataccgaggg tttctaaaat attaagccag ttcttagccg tatattggtc
      541 tatctcggca tcgtccgcaa ttcctttgta gttaacaagt tgtgccgttc ttgccgcaac
      601 tgccgtaata aaccgcatga atttcattgc atctatcgct tcggaaagat cacttacatc
      661 acgttccaaa taggttttga tatatccgga atatacaacc gagcggtctt tatatttgcc
      721 gcttaccaaa gcgggcattc cgcctttcca tatgcgctca aacaattccg gagcggacac
      781 cgatgcaact gctttctgtc gctcaaataa tgtatcaaaa tcagtggtaa aaggaaccgt
      841 tacacctccg gttatttcac tttgagacaa cggtagcatc tgtaaaagtg ctacacgtcc
      901 tgccaaagat tcctgcacac ctctcatcag tttgaatatc tgtgatcccg tcatccagaa
      961 gtccccggga ttgcaacctc tatcaatcag tatttttaca tacggaaata actccggagc
     1021 atactgaacc tcatctataa atatcggagg tttatgaagc tgaaaaaaca ttgcaggatc
     1081 ggtcttggca agcatacggt cgctaatatc atcaagagta acatacgcac gattttcggg
     1141 ctcactcttc atcagttctt taagcatggt ggttttcccg acctgtctcg gacctgtaat
     1201 gaggatagcg ggaaattgct cgcttaatcg tagcaccact tcttccatat gtctttttat
     1261 atagttcatt tcatcatctc cgtttcggct aaaatatggt catattcata ttatagccga
     1321 aaaactaaaa tgtcaatagc attaatttat acccgataca tcaatataca taagcatctt
     1381 accgtcattt accgctaagg tattgtcagg caagaaaaat atttgaccgc cgtttaatgt
     1441 gactgaatcc tgcaacagca cttctttagt tttaaggtct atcagtaata tatgatctgc
     1501 aacaaaagcc catacaagct caaaagtata tccgctttct cctgtaagta ttgccacata
     1561 tcttccgtcc tttgataatg aataatctac tatgtacgga aaatcctctg ccaaactctt
     1621 aatgtccacg gcttcttttt tctcatcatc atatatatac atagttgtct ggcttcccgc
     1681 acctcccgaa accgcagcgg aagttacaaa ttcatccgct tcatttgaaa aaatgtaata
     1741 accccggtcc ctatcatatc tgcctatagc tgtactggaa ccgtcccgta tgtcataagc
     1801 atataggttg aagtagagca ttccttcgcc tgaatgtatt ttctgataaa caaatctgtt
     1861 ttcgtccaat gctttttgaa ataaatagtc ttttaccggc caatcggcga tattcgcact
     1921 taataccgta tacactgtat cggtttcggt atccttaagg tgaatactgt aattttcgtt
     1981 tattgccctc cattttgtgc cgggaatggg tttttcatat gcatcggaag gcatattctt
     2041 tgcaatatat tctcccattg tatctatgta gtcatataaa tgttcaatgt tttttgtgta
     2101 ataaaacatg attacctcat cagccgcacc ggagtatctg tcggtataat ccaacttaat
     2161 acatctggtg tcgggataat cttttgacca tataatttcg tttgtcctgt agttgaccat
     2221 atatagctta ctcacgggct cgggaaggaa aaagactacc ttatcctttc ctacgggaag
     2281 atacttttca gtcggtccgg aatgtgtctg ttcaaaagtg atatcttttg tcaactcgga
     2341 aataatctta ctgtctttca aatcataaga ggtaatccgg tcgctgtatt ttgccaaagt
     2401 ggggatagct tttacatcaa cagtatctgt cacatatgca ccgagactga atctgccgtc
     2461 ggtttttaca tagtcatata gtttttttga agaatctatc atttcatttt ccggtataat
     2521 tcccgaaaga acaaatctgt tgttaatatt gtctgcaaca aatacagcct gcagcataat
     2581 atcgtaattg tttgcttctc ctcttgtcat gccttctata tcgtttgcgg cattaacgga
     2641 aatgcggtat tgttgcgcta ttaaaatatg actgtctcgg tttttgaaac tgtatcttat
     2701 attgtgtacc tctgaatcgg aaatagaaac attacaatca gttattttat aattcacata
     2761 ctttgaacac acatatcgcc ttatccagtc aatcccgtaa tttgtattct cggtcctcat
     2821 gtcaatcgcg atatctttcg ctactatatc cttcctttcc aaagggaagt t
//
LOCUS       DAOPWC010000177         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36110_length_2868_cov_4.583718, whole genome shotgun sequence.
ACCESSION   DAOPWC010000177 DAOPWC010000000
VERSION     DAOPWC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36110_length_2868_cov_4.583718"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKJ65_04600"
     CDS             <1..1020
                     /locus_tag="PKJ65_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNZ41148.1"
                     /translation="KKESLASSSLKYSVSFLTGNYVTLTNMSYEEINRIASMHLSPIN
                     ISVHTTDPELRCTMLNNRHAGDILDKMAVLAEAGTDLNCQIVLCKNVNDKAHLDRTIK
                     DLSCIGQPLKSISVVPAGLTRYRDGLYPLEPFTEEECIQVINQVQKHQKIFKEKSGYN
                     LVYAADEFYIKAGLELPSFGEYDDFYQIENGVGLMTHFIHDAKNYLEEISNSKKLLKR
                     AEKNTKHKSIATGVLAYPFIKELTDKVRMYAKNMKIDVYEIKNNFFGENITVTGLLTG
                     QDISEQLQGKELGEQLLISRTMLKASDEHFLDDMSYKQLGKKLKTTVTVTDNNGEEFI
                     KTLIK"
     gene            1111..2433
                     /gene="der"
                     /locus_tag="PKJ65_04605"
     CDS             1111..2433
                     /gene="der"
                     /locus_tag="PKJ65_04605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNZ41149.1"
                     /translation="MAQPIVAVVGRPNVGKSAFFNYLAGRRISIVEDTPGVTRDRIYT
                     KCDWCGREFVLIDTGGIEIGSSDEMYKQMKRQAEVAIDTADFIIFMTDIKSGLTAGDK
                     EVASLLHKSGKPVVIAVNKADSPGEPPAELYEFYNLGLAEIFPISAMHGLGIGELLDY
                     VMEHLPDIQEQSDDDSIHVAIIGKPNSGKSSLLNRITGEERAIVSPVAGTTRDALDSL
                     VIREEKKYTFIDTAGIRRSSKIFSDIEKYSIIRAWSAIERSDVCILIVDASEGITEQD
                     SKIAGFAHEQGKAAIVALNKWDLVEKDNKTYQKFVNSVRAELPFMQYAPVISISSLTG
                     KRVENLFPLIDKVYENANRRISTGVLNDVLNDAIAMVQPPSDKGKLLRIYYMTQVSTQ
                     PPTFVIFVNSKDLMHFSYERYLTNTLRDTFDFEGTPIRFIIRERGKEN"
     gene            2430..>2868
                     /gene="plsY"
                     /locus_tag="PKJ65_04610"
     CDS             2430..>2868
                     /gene="plsY"
                     /locus_tag="PKJ65_04610"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNZ41150.1"
                     /translation="MNILLHVISAVFGYLVGSLNSAILVSRFYKKDIRNEGSKNAGLT
                     NVLRVLGKKAAIAVLLLDLLKGVITTGLGYILVKDQLSGALVAGIFTVIGHIFPVFFK
                     FKGGKGILISLSVLLMTDWRIALIGLGIFIIIVGLTRYVSLGSI"
BASE COUNT          931 a          521 c          668 g          748 t
ORIGIN      
        1 aaaaaagaaa gcctggcatc atcatccttg aagtacagcg tttctttttt gaccggaaac
       61 tatgtcacat tgaccaatat gtcgtatgaa gaaattaaca ggatagcttc tatgcattta
      121 tctcccataa acatatcggt acacaccacc gatccggaat tgagatgtac catgctgaac
      181 aacagacatg caggagatat tttggataaa atggcggtac ttgcagaagc gggaacagac
      241 ttaaattgcc agatagtact gtgtaagaat gtaaacgata aggctcattt agacagaaca
      301 ataaaggacc tttcatgtat aggacagccg cttaaaagca tttccgtagt acccgcaggg
      361 cttacccgtt acagggacgg gctgtatccg ttggagccgt ttaccgaaga agaatgtatt
      421 caggttataa atcaggttca aaaacatcag aaaatattca aagaaaagtc gggatataac
      481 cttgtttatg cggcggatga attttatata aaagccggct tggagcttcc ttctttcgga
      541 gaatatgatg atttttatca gatagaaaac ggtgtaggat tgatgacaca cttcatacac
      601 gatgcaaaaa attatcttga agagatatca aacagcaaaa aacttcttaa aagggcggag
      661 aaaaacacaa agcacaaaag tatagcgacc ggagtgctgg cttatccttt tataaaagaa
      721 cttacggata aagttcgtat gtacgcaaag aatatgaaaa tcgatgtata tgagattaaa
      781 aacaatttct tcggagagaa tataacggtg accggactgc tgacaggaca ggacatttcg
      841 gaacaattgc aaggaaaaga actaggggag cagctcctca tttcccgtac catgctgaag
      901 gcaagcgatg aacattttct tgatgatatg tcctacaaac aactcggcaa gaaactcaag
      961 acgacagtta ccgttacgga caataacgga gaagaattca taaagacact tataaaatag
     1021 atacacacac ttataaacgg gcaattcttg agaaaacacc atgtatatgg tattatttat
     1081 ccagtttatt tgaaaggcgg agcggggaat ttggcacagc cgatagtggc cgtagtgggc
     1141 agacctaatg taggtaaatc agcatttttc aattatctgg caggcagaag gatatccata
     1201 gtggaagata ccccgggtgt gacacgcgac agaatataca ctaaatgcga ttggtgcggt
     1261 cgggagtttg tgcttatcga taccggaggt atagaaatcg gttcttctga cgaaatgtat
     1321 aaacaaatga aaagacaggc ggaagttgcc attgacactg ccgactttat aatcttcatg
     1381 accgatataa agagcggact tactgcgggg gataaggaag ttgcatcact gctacataaa
     1441 tcgggaaagc ctgtggtaat tgcggtaaat aaagcggatt cgccgggaga gcctcctgcg
     1501 gaattgtatg aattctataa cctgggactg gcggaaatat ttcccatatc ggctatgcac
     1561 ggactgggaa taggcgaact tttagactat gttatggagc atcttcccga tattcaggag
     1621 caatcggatg atgacagtat acatgtggca ataataggta aacccaactc gggcaaatct
     1681 tctttactga acagaataac cggagaagag cgggccattg tcagtcccgt tgccggaacc
     1741 acacgcgatg ctttggattc attggtgata agggaggaaa agaaatatac ctttatcgat
     1801 acggccggta tcaggcgaag cagtaagata ttttccgaca tagaaaagta cagtatcata
     1861 agagcctggt cggccatcga acgatcggat gtatgcatac ttatcgtgga tgcatcggaa
     1921 gggattaccg aacaggattc caagatagcg ggatttgccc atgaacaggg taaagctgca
     1981 attgtcgcat tgaacaagtg ggaccttgtg gaaaaagaca acaagaccta tcagaaattc
     2041 gtaaattccg tcagggcgga gttgccgttc atgcaatatg ctccggtcat atctatttca
     2101 tcacttaccg gaaaacgagt ggagaatctg tttccgctca tagacaaagt atatgaaaat
     2161 gcaaacagaa gaatatccac aggggtactg aatgatgttc tcaatgatgc aatagctatg
     2221 gttcagccgc cgtcggataa aggaaaactt ttaagaattt actacatgac tcaggtatcg
     2281 acacaaccgc ctacatttgt tatctttgta aacagtaagg atctcatgca cttttcctat
     2341 gaaagatatc tgaccaacac actaagagat acgtttgatt ttgaaggtac acctataagg
     2401 tttataatac gggagcgagg taaagagaat tgaatatttt actgcatgta atatcggctg
     2461 ttttcggata cttggtgggt tctttgaact ccgccatact ggtttcacgc ttttataaaa
     2521 aagatataag gaatgagggc agtaaaaatg cgggtcttac aaatgtgttg agagttttgg
     2581 gcaaaaaggc tgcgatagcg gtacttttgc tggatttgct taaaggagtt ataaccaccg
     2641 gtttgggata tatattagtt aaagaccaac tgtccggtgc acttgtagcc ggtatattta
     2701 ccgtcatagg acatatattt cccgtgttct ttaaattcaa gggcggaaaa ggcatactga
     2761 tttcactttc cgtgttactt atgaccgact ggagaatagc acttataggg ctggggatat
     2821 ttataataat cgtgggactg acgagatatg tttctttagg ttccatta
//
LOCUS       DAOPWC010000178         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36232_length_2862_cov_2.691486, whole genome shotgun sequence.
ACCESSION   DAOPWC010000178 DAOPWC010000000
VERSION     DAOPWC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36232_length_2862_cov_2.691486"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKJ65_04615"
     CDS             complement(<1..180)
                     /locus_tag="PKJ65_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41151.1"
                     /translation="MKKKKTTKSYDDQIHFHGRIWMLGALVLFSLFPTAICVIYNAWP
                     SAGPLFKALAGVAPIF"
     gene            complement(190..924)
                     /locus_tag="PKJ65_04620"
     CDS             complement(190..924)
                     /locus_tag="PKJ65_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HNZ41152.1"
                     /translation="MTFDPNSKLMYIMVAIVLLYVTAQAVFFLVKAIKRAKELNISSS
                     TVKKTILSSAVFSIAPAISILMGVLTMAGFLGLPFPWLRLSILGALTYEVTAASSAAK
                     SLGITDLSTLVTDPKVFATMAWVMHLGIFTGLILVPLFLKKIQRGLIKIKERDEKWSD
                     AFLTALFLGMISAFLGLIFADIKSGLAGWIPVFVMVVSALIMSVLGILVKKFKIKWLE
                     DFALPITMLVSMALSIPITNLIESLI"
     gene            1153..1398
                     /locus_tag="PKJ65_04625"
     CDS             1153..1398
                     /locus_tag="PKJ65_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41153.1"
                     /translation="MKYYHRTMGYLYGKKERKGSAAAEAAIVLPLILLLVISVMMMIV
                     KISCEEIEYTEYDLTSGIRTYDGIKRKGDIIFEAFFE"
     gene            1379..>2862
                     /locus_tag="PKJ65_04630"
     CDS             1379..>2862
                     /locus_tag="PKJ65_04630"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HNZ41154.1"
                     /translation="MKPSSNSKKGVITAFVAVITPVLILLVLVLSDIYFAKNALNAAK
                     NAAKASLGCVLSQYSTYMKERYSLYGYEMSDREAALRITEALNNSSISRGLFEMEIVN
                     VEVERNKPLASYPVISAQINRLMSDEIFKTVLAETNERIEMFSKIKDVIRTLNVKMQI
                     DELIGGLKNAHTLLKETVEEINSSPYYHDLMEIIRYADSLYGEMLSYMKSGDKEQTDT
                     AQVLKKQVTDILKTNVDNIIYVLKEYNKKAVNLLNEMINTYADIHILSENLKGIVDGI
                     EDCPEQLKAVLKICTDTVYKMETSLSVSVLQNIKAVLDRNVDCLEQTMKHMLDTVSEE
                     KDVSLYDVFCDGFEMYYDAVYNGDVLDGFFASVIDDIVKDQRSLLKKMADEVLESIII
                     EDIRIDDFINLVSEGYEDTYIFQADATGNTSKSISGSFTDLSEMLSQIKEDIMINEYI
                     MLYMNGFTDSDTDQGENRYLDAEIEYIIFGNRDNRKNVQSCKASL"
BASE COUNT          929 a          490 c          620 g          823 t
ORIGIN      
        1 gaagataggg gcaactccgg caagagcttt gaagagagga ccggcagacg gccatgcgtt
       61 atatataaca catatagctg tcggaaacag tgagaaaaga actaaagctc ccaacatcca
      121 gattcttccg tggaaatgta tttgatcatc atatgatttt gttgttttct tctttttcat
      181 gtccgcctcc taaattaaac tttctattaa attggtaatg ggaatggaaa gggccattga
      241 aacaagcatt gttatgggaa gagcgaaatc ttccagccac tttatcttga atttcttaac
      301 cagtataccc agaaccgaca taatcaatgc cgaaaccacc attacgaata ccggtatcca
      361 tcctgcaaga ccggatttta tatctgcaaa gataagtccc aaaaacgcag atatcatacc
      421 taaaaacaat gcagtaagaa aagcatcact ccatttttca tcccgttcct ttatcttaat
      481 cagacctctt tgaatttttt tgaggaataa ggggacaaga atgagtccgg tgaatattcc
      541 caagtgcatt acccatgcca ttgtggcaaa gactttaggg tcggtaacca aggtggacag
      601 gtcggtgata cccaatgatt tggctgcgga agatgccgcg gtaacttcgt atgtaagtgc
      661 tcccagaatg gataaacgca gccatggaaa gggcagtccc aaaaagcccg ccattgtcag
      721 cacacccatc agtatggaaa tcgccggtgc aatggagaag actgcactgg aaagtatggt
      781 cttttttacc gttgatgaac ttatattcaa ctctttagcc cttttaatag cttttacaag
      841 gaaaaagacc gcctgagcgg taacataaag taagactatt gctaccatta tgtacattaa
      901 tttggaatta gggtcaaatg tcatatttac ctcccgttat taatatttac tacagattta
      961 tgtcaattat acacttatta atgtttcttg agtaggtgaa atatatattt ttatctgaat
     1021 tttacttttt ttccgatttt ctcttatttt tattacattt caaagcatta cacattttat
     1081 aggggtaatt catatagaaa ttatatttac agtttctgta aatatacagt tttgtctgtg
     1141 taataatacc gggtgaaata ttatcatcgt accatgggat atttgtacgg taaaaaagaa
     1201 agaaaaggca gtgcggcagc ggaagcggca atagttcttc ctctcatact tttgctggtt
     1261 atttccgtaa tgatgatgat tgtgaagata agttgcgagg agattgagta tacggaatat
     1321 gaccttactt ccgggataag gacatatgac ggtataaagc gtaaagggga tataatcttt
     1381 gaagccttct tcgaatagta aaaaaggagt gataacggca tttgtggcgg ttataacgcc
     1441 ggtactgatt ctgttggtat tggtgctatc cgatatatat tttgccaaaa atgcactgaa
     1501 tgcggcgaaa aatgccgcta aagcatcctt aggatgtgta ttgagccaat acagcacata
     1561 catgaaggag cgctattcac tatacggcta tgagatgagc gaccgggagg ctgctttacg
     1621 tataaccgaa gctctgaata actcttcgat aagcagagga ctttttgaaa tggaaatagt
     1681 gaatgtggaa gtagagagaa acaaacctct ggcaagttat ccggtcatat ccgctcagat
     1741 aaaccgcctt atgagcgatg aaatattcaa aaccgtttta gccgagacaa acgaaagaat
     1801 agaaatgttt tccaagataa aggatgttat caggaccctt aatgtgaaga tgcagataga
     1861 cgaattgata ggcggattga aaaacgctca tactttgttg aaagaaactg ttgaagagat
     1921 aaattccagc ccgtactatc atgaccttat ggagatcata cgttatgcgg attcgttgta
     1981 cggtgagatg ctttcgtata tgaaaagcgg agataaggag cagacggata ccgctcaggt
     2041 actgaaaaaa caggtaacgg atattttgaa aaccaatgtg gataatatca tatatgttct
     2101 gaaagagtac aataaaaaag ccgttaacct gttgaatgaa atgattaaca cctatgcgga
     2161 catacacata ctgtcggaaa atctgaaagg tatagtggac ggtattgaag attgtcccga
     2221 acagttaaag gccgtattga agatatgtac cgataccgtt tataaaatgg aaacttcctt
     2281 atcggtgtcg gttttgcaaa acattaaagc ggtattggac agaaatgttg actgtctgga
     2341 acagaccatg aagcatatgc tggatactgt aagtgaagaa aaggatgttt ctctatatga
     2401 tgttttttgt gacggttttg aaatgtacta tgatgctgta tataacgggg atgttctgga
     2461 cgggtttttt gcatcggtca ttgatgatat agttaaagac cagcggtctc tgttaaaaaa
     2521 aatggcggat gaagtactgg aatcaatcat aatagaagac ataaggatag atgattttat
     2581 caatctggta tcggaaggtt atgaggatac atatattttc caagccgatg ccaccggcaa
     2641 cacctcaaaa agcatatccg ggtcttttac ggatttatcc gagatgctgt ctcagattaa
     2701 agaagatatc atgattaacg aatacataat gctgtatatg aacggtttta cggattcgga
     2761 taccgatcaa ggtgagaaca gatatctgga tgccgaaatt gagtatatca ttttcggaaa
     2821 cagagataac agaaagaatg ttcaatcgtg caaagcttct ct
//
LOCUS       DAOPWC010000179         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36561_length_2842_cov_3.553283, whole genome shotgun sequence.
ACCESSION   DAOPWC010000179 DAOPWC010000000
VERSION     DAOPWC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36561_length_2842_cov_3.553283"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2322)
                     /locus_tag="PKJ65_04635"
     CDS             complement(<1..2322)
                     /locus_tag="PKJ65_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41155.1"
                     /translation="MKEKKKSKRLLRTSVMLLVFLLSLCSALSYGIWSSAKTCINKAM
                     GGKISLAWLKESEFLPNSKSATQAYPYSLLQEAYTAANSSLNVDSVDIRFQCMAHAGV
                     NTVVSDECGVSVLASVDKPYKDAVIVGTCEAIELKSTSKGTDSDGVSVPYFGYVVTLK
                     IDKNLSPVLDSDYPYSYVRIMSSQVMEDFSDFFIVGSKYIAIGTVSYLYIPFEEDQAP
                     YFNIGEADTLLPVTEVKFKESMYEKIGVPKGTKISYGRSYYDLTSCYLSAYTDSSFLS
                     YAKIENSLEEFLSSDENSRWNNIITSKKTAKESVKLVTVNNIESVPLFRSGRAYITQG
                     SSFTDKDYSSGNKVCLISEQLAKSNNIKVGDVLDMTLWNAGYDLQTGPLGASWSTKPY
                     SNKHTLSEKEGYTVTGIYTDDDAWNTQCCAFTPNTVFIPDSCISDSVKTYSSLTDLGS
                     DRYTGMPDSRTYILKTGKASDFEAEMEKAGYGGMFYYCENSAPSLPYLNSLSDTFRTV
                     TYISTSAFAVSILIIAIYAAVKNIEKTDKKTRLRTVLTVVIISVISIAVSAVISLSFY
                     GIVINAANKNISSKEITEQVLKTEPTLPAPDIKTEQLIIDTAPTPLLMAFAVQTGTLL
                     LVLCISSALCFVVKSGQKKKKVPYRLKKGDGVKGGMPAVLWYSIAQSARSTVMVLLMT
                     VLVTLSGIFMFLSVSTWITAKRSISEAVKAYTTIAVAAENEFVKNVRGFDTGVYDYSS
                     LVDLYETAEKSEHIKYVDIRDKCMAQSNTIKTIS"
BASE COUNT          803 a          577 c          561 g          901 t
ORIGIN      
        1 cgatatcgtt tttatagtat tactctgcgc catgcattta tcgcgaatat cgacatattt
       61 tatatgctcg gatttctccg cagtttcata taggtcgaca agcgaactgt aatcataaac
      121 cccggtatca aaacccctta cgttttttac aaattcgttt tccgcagcta cggcaatggt
      181 agtatatgct tttaccgctt cggatatgct tctttttgcc gttatccatg tgcttacact
      241 taagaacata aatatgccgg agagtgttac aagcactgtc ataagaagta ccattacagt
      301 ggaccttgcg gactgtgcaa tactgtacca cagtacggca ggcattccgc cttttactcc
      361 atcacctttt tttaatctgt acggcacctt tttcttcttt tgtccgcttt ttacaacaaa
      421 gcataatgct gatgatatgc aaagaacaag aagaagtgtt cccgtctgaa cggcaaatgc
      481 cataagtaaa ggagtaggag cggtgtcaat tatcaattgt tccgttttta tgtcgggagc
      541 cggaagagtg ggttctgtct ttaatacctg ttcggtaatc tcctttgatg atatgttttt
      601 attcgcagca ttaatcacaa taccgtaaaa agaaagagat ataaccgcac ttacggcgat
      661 agaaataaca gagattatga caaccgtaag aaccgtacgc agtcgtgttt tcttatcggt
      721 tttttctatg tttttaacag cggcatatat ggctataatc aaaattgaaa ctgcaaaagc
      781 agatgtggaa atatatgtta ctgttcggaa tgtgtccgac agggaattga gataaggtaa
      841 ggaaggagcg ctgttttcac agtaatagaa cattcctccg tatccggctt tttccatctc
      901 agcctcaaaa tcggatgcct tacctgtttt gagaatatat gtccttgaat cgggcatacc
      961 ggtatatctg tcactgccca aatcggtcag tgaagaatat gtctttacag aatcactaat
     1021 acaggagtcg ggtataaata cggtgttggg ggtaaaggcg cagcattgtg tattccatgc
     1081 gtcatcatcg gtatagatgc ccgtaaccgt ataaccctct ttttccgata atgtatgttt
     1141 gttgctatac ggtttggttg accacgatgc tcctaaagga cctgtctgaa ggtcatatcc
     1201 ggcattccat aatgtcatat ccaaaacatc cccgacttta atattattac tctttgcaag
     1261 ctgttcgctt atgagacaaa ccttgttacc ggaagaatag tctttgtcgg taaaagaact
     1321 tccctgtgta atatatgctc tgccgcttct gaaaagagga acactttcaa tgttgtttac
     1381 tgtaactaat tttaccgatt cctttgccgt cttcttactt gtaatgatat tattccatct
     1441 gctgttttca tcggaagaaa ggaactcttc caaagaattt tctatttttg cataagaaag
     1501 aaatgaagaa tcggtatatg cggaaagata gcatgatgta aggtcataat aggagcgtcc
     1561 gtaagatatc tttgttccct taggtacacc tattttttca tacatacttt ctttaaactt
     1621 tacttcggtt acgggaagca acgtatcggc ttctcctatg ttaaagtatg gtgcctggtc
     1681 ttcctcaaag ggaatgtaga gataggatac cgttcctatg gctatatact tagagccgac
     1741 aataaagaaa tcgctgaaat cttccataac ctgagaagac attatcctca catatgaata
     1801 agggtagtcg gaatcaagaa caggagataa gtttttatct atcttaagtg taacgacata
     1861 accgaaatac ggtaccgata caccgtcact gtcggttcct tttgatgtgc tttttaattc
     1921 tatagcttcg caagttccca caattacggc atccttgtac ggtttgtcta cagatgcaag
     1981 aacggaaaca ccgcactcat cggataccac ggtattcact ccggcatgag ccatgcattg
     2041 aaacctgata tcgaccgaat ctacattcaa agaactgtta gcagcagtat atgcttcctg
     2101 cagcaggctg tacggataag cttgggtagc cgatttactg ttaggcagaa attcgctttc
     2161 tttcagccag gctaaagata tttttccgcc catagctttg tttatgcagg ttttcgctga
     2221 gctccatatc ccgtaagaaa gagcggaaca taagcttaac aaaaaaacaa gcaacataac
     2281 ggatgtcctt aatagccgtt tacttttctt tttttctttc acttgttgcc tcctgttaag
     2341 gttattatat gctgtatagt atgtttatgg caaagtgtta tcccgaaaga aacgggataa
     2401 cacatcagtt tttcggatat actaccactc tttatctgta tacatcaatt cttataccac
     2461 aatttgtgca atcgtggtac caccattgtt ctcctgaagg gagtgttccc atgtagtata
     2521 aaggaccaaa tacgtgaggt ggataagtag tttcttgagc tggagttggt ttagggtaat
     2581 tacatttctg acattgttca tatccgcttg caattctttc gcaataggta tcatcatagt
     2641 aataataggc tgcagattgt tcgacgaagt caaagtcatg agcggtatac agcggagttg
     2701 tcacagtaca ttgtgcagga catttaatgc atttgtagta ctccatcttg ttatgagcac
     2761 attgacttgg actgtgataa tagtaatccg aactttcttc ctcatacaaa aagtcccact
     2821 catgattaca ttccgcagat ac
//
LOCUS       DAOPWC010000180         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36635_length_2838_cov_3.986346, whole genome shotgun sequence.
ACCESSION   DAOPWC010000180 DAOPWC010000000
VERSION     DAOPWC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36635_length_2838_cov_3.986346"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="PKJ65_04640"
     CDS             complement(<1..1991)
                     /locus_tag="PKJ65_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ41156.1"
                     /translation="MNPFIQVFSDIPQLIGVADCIALKQTPVGLSGPGTSLCPLISHI
                     TASSVSRKLLIVTHSRTAALSVHRNLISFYGDDAVHIPTRELMLFDIDAYSGNDKQAR
                     LKALYRIATGDFKAAVACTEALTVGTASRQVFLQSVINLKKGELHDMQTLSDILMRLG
                     YVRKALVEEQGQYSIRGGILDIFSCNYHNPIRCEFFGDEIDSIRFFDADSQRSVETVN
                     NVVIIPFSEFILDWNDESMLKERITDSWDLQQFNEGTYAKIMDRYYAATDKCAMTGTY
                     FDKDILTVIHDDLRCHSVADTVYKEYANACVNLLQKKKTFKDACQMQRDIYSLYSCLK
                     PQLVFVYEKSNYSIPLYRDFDITFEETCSYKGDMELFLNDIAEYRAANMRIFISSDRK
                     KHTEYLKGFFEDNGYTVHIIKPKENPTVIPRHIYIIEDGYGESFVSKEAKIACVSVSR
                     IFRKSTRGKSRQIKDADFFAEIQPNDYVVHDHHGIGIYLGIEKIEIDGIIKDYIKIAY
                     KNNDMLYVPVNKMDLVQKYVGTSDRAPRITGLGGRDWEKQKSKVRSAVRDIAKELIEL
                     YGKRSSVKGHAFSEDTPWQISFENEFEYEETQDQIQCVEEIKADMESTKVMDRLLCGD
                     VGYGKTEVALRAAFKAVMDGKQVAFLAPTTMLALQHYNN"
     gene            complement(1996..2580)
                     /gene="pth"
                     /locus_tag="PKJ65_04645"
     CDS             complement(1996..2580)
                     /gene="pth"
                     /locus_tag="PKJ65_04645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ41157.1"
                     /translation="MDRYLIAGLGNIGLRYRNTRHNIGFMAVDKLADVYSIKVKKNLC
                     QANVGEGFINNIPVVLAKPKTYMNLSGQSIRALTDWYKVDISGLIVIYDDMDIPVGEL
                     RIRYKGSGGSHNGMKSILSHLGTDRFIRIRVGIGKHEHIDAIDYVLGRFPKSEKEIID
                     NTLQDLIKAITCIVTEGIDIAMNRYNKKNDRGDV"
     gene            complement(2580..>2838)
                     /locus_tag="PKJ65_04650"
     CDS             complement(2580..>2838)
                     /locus_tag="PKJ65_04650"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF025923.2"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ41158.1"
                     /translation="DMGAKRVLVYATHGVLSADAVTKIKNSKIERMYLLDTVPIPKEK
                     MIDKIEVVGVAKLFSEAIYKIYTNTSMSALFKMPGGGRNEK"
BASE COUNT          737 a          670 c          518 g          913 t
ORIGIN      
        1 aagttgttgt agtgctgcaa agcaagcatg gtggtgggag caagaaaggc cacctgttta
       61 ccgtccatta ccgccttgaa tgccgcacgt aaagccactt cggttttacc gtagcctaca
      121 tccccgcaca acagacgatc cataactttt gtcgattcca tatcggcttt tatctcttcc
      181 acacactgaa tctgatcctg tgtttcctca tattcaaatt cgttttcaaa agaaatctgc
      241 cacggagtat cttcgctgaa agcatgccct tttacggaac ttctctttcc gtacagttca
      301 atcagttctt ttgcaatatc gcggacggca cttctgactt tactcttctg cttttcccag
      361 tcacgaccgc ctaatccggt aattctcgga gctctgtccg atgtgcccac atatttctgg
      421 accaggtcca ttttgtttac cggtacatat aacatgtcgt tgtttttata tgcaatcttg
      481 atatagtcct taataattcc gtctatttca atcttttcta tacccagata tatgcctatt
      541 ccgtgatgat cgtgcacgac atagtcattt ggttgaatct cggcgaagaa atctgcatcc
      601 tttatctgcc ggcttttacc tcttgtactc ttgcggaata tacggcttac cgaaacacag
      661 gctatctttg cttctttgga cacaaaactc tcaccgtaac cgtcttcaat tatatatata
      721 tgcctgggta taacggtggg attttcctta ggcttaatga tatgtaccgt atatccgtta
      781 tcttcaaaga aaccttttaa gtattcggta tgtttttttc tgtcggaaga aatgaatatc
      841 cgcatattgg cggctctgta ctcggctata tcgttcaaaa acagttccat atctcccttg
      901 taggaacatg tttcttcaaa cgtaatatca aaatcccggt aaagaggaat actgtaattc
      961 gacttctcat atacaaatac caattgcggt ttaaggcagg aatacagtga atagatgtct
     1021 ctttgcatct ggcatgcatc tttgaaggtc tttttctttt gcaaaagatt gacgcaggcg
     1081 tttgcatatt ctttgtatac ggtatccgcc accgaatgac atcgcaaatc gtcatgtata
     1141 accgtaagta tgtctttatc gaaatatgtg ccggtcatag cacatttatc ggtcgcggca
     1201 taatatctgt ccataatctt tgcataggta ccctcgttaa actgctgcaa gtcccatgag
     1261 tcggttatcc gttctttgag catactctca tcattccaat ccaagataaa ctcactgaaa
     1321 ggtattatga caacattatt aaccgtttct acagatcttt gcgaatcggc atcaaaaaac
     1381 cttatactgt ctatctcatc tccgaaaaac tcacacctta tcgggttgtg gtaattgcag
     1441 gaaaatatat ccaatatgcc gccccttata ctgtactgtc cctgttcctc aacaagagct
     1501 ttccttacat atcctaaacg catcaatata tcggaaagag tctgcatatc gtgcaattca
     1561 cctttcttga ggtttataac gctttgcaaa aatacctgtc tggaagccgt acctactgta
     1621 agtgcttcgg tgcaggcaac agccgcctta aaatcgcctg ttgctattct gtatagcgct
     1681 ttaagcctgg cctgtttatc atttccgctg tatgcgtcaa tatcaaaaag catcaactct
     1741 ctggtcggta tatgtaccgc atcatcaccg taaaaagaaa tgaggttacg gtgtacagat
     1801 aaagcggcag ttctggaatg agtcactatt aacagctttc ttgataccga tgaggctgta
     1861 atgtggctta taagggggca taaagaagtc cccggtccgg acaatcctac gggggtctgt
     1921 ttcaacgcta tgcagtccgc tacccctata agctgaggga tatcggaaaa gacctgtatg
     1981 aaaggattca tttagctaaa catcacctct gtcattcttc ttgttatatc tgttcatagc
     2041 tatatctata ccttccgtca caatacatgt tattgctttt atcaagtcct gtaaagtgtt
     2101 gtctataatc tctttttcgc ttttgggaaa tctgcccagc acataatcta tagcatcaat
     2161 atgctcatgc tttcctattc ccactcttat gcgtataaac cggtcggtac ccagatgact
     2221 gagaatcgat ttcattccgt tatgcgaacc gccacttccc ttatagcgta tacgaagttc
     2281 tcccacagga atatccatat cgtcatatat gacaataagt ccggaaatat ccaccttgta
     2341 ccagtcggta agtgcgcgta tactctgacc gctcaagttc atgtaagtct taggtttggc
     2401 aagaacaacg ggaatgttat tgataaagcc ttccccgaca ttggcctgac agagattttt
     2461 tttaaccttg atagaataaa catcggctaa tttgtcaacc gccataaaac cgatgttgtg
     2521 tcttgtgttt ctgtatctga gccctatatt tcccagcccc gctattaaat atctgtccat
     2581 cacttttcgt ttcttccgcc gcccggcatt ttgaaaagag cggacatgga ggtattggta
     2641 tatatcttgt agatagcctc ggaaaacaat tttgcaacgc ctactacctc tattttatct
     2701 atcatctttt ccttgggtat cggtacggta tccagaagat acattctctc aatcttggaa
     2761 ttcttaatct tggttacggc atcggccgat aatactccgt gtgttgcata taccaggact
     2821 ctttttgctc ccatatcc
//
LOCUS       DAOPWC010000181         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36834_length_2828_cov_3.410025, whole genome shotgun sequence.
ACCESSION   DAOPWC010000181 DAOPWC010000000
VERSION     DAOPWC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36834_length_2828_cov_3.410025"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKJ65_04655"
     CDS             complement(<1..288)
                     /locus_tag="PKJ65_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ41159.1"
                     /translation="MDNVISYLRELNFVTMIIRIVLAFLCGGLIGMQRERHGRSAGFR
                     THIIVCIGSALTAMTGIFMSDILGFDTDPMRLSAQVLSGIGFLGVGTILVTG"
     gene            complement(306..2207)
                     /locus_tag="PKJ65_04660"
     CDS             complement(306..2207)
                     /locus_tag="PKJ65_04660"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNZ41160.1"
                     /translation="MHYGFIKVAAASPSLRVADCKYNCDGIIKTIKKAYDRKVELLVF
                     PELNITGYTCDDLFSHKLLIDQALTGLLDIVKASDGKEILIVTGLPVKAEGYLYNCAA
                     VVYNGNILGIVPKTHLPNYNEYYEMRTFRSAPKENFVIDINGTSVPFGTKLIFRNTLM
                     SDCSLAVEIGEDLFAPVSPGSLHAMNGAAVIANLSANNELLDKADYRKTIIEAQSRKS
                     ICAYIFCNASEQESTTDMVFSGHKMIAENGTVFSEALPFAGEYIETEIDLERLSHDRK
                     SRLPVAEQHDSGYKIIPFTMKPVECRISRTIKKHPFIPEDNDTMAKRCDLILNIQAAG
                     LKKRMEHTNCKTAVIGISGGMDSTLALLVTHRAFKDMGRSPSDIVCISMPCFGTTKRT
                     KDNASKLSRILNTDFRVINITASVKRHLADIGHDGATTDAAYENAQARERMQVLMDIS
                     NMTNGMVIGTGSLSEQAIGWSTYNGDHMSMYNVNTSVPKTLVRHIIRYIADHSESKLK
                     NVLYDILDTPISPELIPGVNGEIIQKTESIVGPYELHDFFLYYFLRWGFTPKKILMLA
                     TYAFKDDYSADEIKKWLKIFLRRFFLHQFKRSCSPNGPKIGSVALSPRGDWRMPSDAS
                     SEGWMKNLD"
     gene            complement(2304..>2828)
                     /locus_tag="PKJ65_04665"
     CDS             complement(2304..>2828)
                     /locus_tag="PKJ65_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41161.1"
                     /translation="SVGLSYWEINLINALYFLIHIFFGKALTKSLNKNSRYRILAYAL
                     LLQGPTYIMYAFVTSENYIGLWVAVRLMQHVLGVIINYIIASLLNDNIPESDRTNHIS
                     FYNLAVHMSCFFSMMLGTLCFWRIGNNVMDIFGFKLAGIPIMLAISGIAQILVGLLCR
                     ILNDKLLADDLQNG"
BASE COUNT          789 a          637 c          536 g          866 t
ORIGIN      
        1 accggtaaca agaatggtac ctacccccaa aaatcctatg ccggaaagta cctgagcgga
       61 aagtcgcatg ggatcggtat caaaccccaa tatatccgac atgaagatac ctgtcattgc
      121 agtcagcgcc gaccctatgc atactatgat atgtgtcctg aatcctgccg aacggccgtg
      181 cctttccctc tgcataccta taagtccgcc gcaaagaaaa gccaacacaa tccttataat
      241 catggtcaca aaattaagtt ctctgagata agaaatcaca ttgtccatgt cagacctcct
      301 ttctttcagt caagattctt catccatccc tcagaagatg cgtcgctcgg catgcgccag
      361 tcccctcttg gagaaagtgc aacagaccct attttaggtc cgttgggact gcagcttctc
      421 ttaaactgat gcaggaaaaa tcttcttaaa aatatcttca gccatttttt aatctcatcg
      481 gcggaataat catctttaaa cgcatatgtt gcaagcataa gtattttttt cggtgtaaat
      541 ccccagcgga ggaaatagta taagaagaaa tcatgcagtt cgtaaggtcc tacaatgctt
      601 tcggtctttt gtataatttc accgtttaca ccgggtataa gttcgggact tatgggtgta
      661 tccagaatat catacagtac gtttttaagt ttgctttccg aatggtcagc aatgtaccgg
      721 ataatatgtc tcaccaatgt tttgggcact gaagtattta cattatacat ggacatgtgg
      781 tctccgttgt atgtactcca gccgattgcc tgttccgaaa gactgccggt tcctataacc
      841 ataccgtttg tcatattgga aatatccatc aatacctgca ttctttcccg tgcctgtgca
      901 ttttcatatg cggcatcggt tgttgctccg tcatgcccta tatccgctaa atgccttttt
      961 accgacgcgg taatgtttat tactctgaaa tcggtattaa gaatcctgct cagtttgctt
     1021 gcgttatcct ttgtcctttt agtggttccg aaacagggca tggatataca cactatatcc
     1081 gaaggagacc ttcccatatc cttaaatgct ctatgtgtga caagcaatgc aagcgtggaa
     1141 tccatacctc ccgatatacc tataaccgcg gttttacagt ttgtgtgttc cattctcttt
     1201 ttaagacccg ctgcctgtat atttaatatc aaatcacatc gctttgccat cgtatcgttg
     1261 tcttccggta tgaaaggatg tttttttatt gtccgggata tccgacattc aacaggcttc
     1321 attgtaaacg gtataatctt atagccgcta tcatgctgtt ccgctacggg caaccggctc
     1381 tttctgtcgt gagacaggcg ttccagatct atttcggttt caatgtattc accggcaaaa
     1441 ggcagagctt cggaaaaaac cgttccgttt tccgctatca tcttatgacc ggaaaacacc
     1501 atgtccgtag tggattcctg ttcggatgca ttgcaaaaga tgtaagcaca tatgcttttt
     1561 ctcgattgtg cttcaataat agtctttctg taatcggctt tatccagaag ttcattgttt
     1621 gccgaaaggt ttgctataac cgcggctccg ttcattgcat gcaaagaacc gggacttacc
     1681 ggagcaaaca aatcctcacc tatttccaca gcaagcgagc agtcgctcat cagtgtattt
     1741 cggaatatga gttttgttcc gaacggcacc gatgtgccgt tgatatctat tacgaagttc
     1801 tccttcggtg cacttctgaa tgtacgcatc tcataatatt cgttatagtt gggaagatgg
     1861 gttttgggaa caatacccag tatatttccg ttatacacta ctgcggcaca attatacaga
     1921 taaccttccg ccttaaccgg aagtcccgta acgataagta tttctttgcc gtccgatgct
     1981 ttgacaatat ctaaaagacc ggtcaatgcc tgatcgataa gaagcttatg tgaaaaaaga
     2041 tcgtcacagg tatatcccgt tatgttaagt tcgggaaata ccagaagttc aacctttctg
     2101 tcatatgcct ttttaatcgt tttaataatg ccgtcgcaat tgtacttaca atccgcaact
     2161 cttaatgatg gacttgccgc cgccacttta ataaatccgt aatgcatttt aatacctctt
     2221 cttatataga ttatacactg tttaaagtaa aattaacata tttgtgttat gttgataagc
     2281 tttttggtaa taaggaaaca tatttatccg ttttgtaagt catcggcaag taacttatca
     2341 tttaatatcc gacagagcag tccgactaaa atttgagcta tgccgcttat tgccagcata
     2401 atgggaatgc ccgccaattt gaagccgaaa atgtccatga cgttattccc tatacgccaa
     2461 aagcataaag tacccaacat catactaaag aaacaggaca tatgtactgc caggttatag
     2521 aaggaaatat gatttgttct gtcgctttcc ggaatattgt catttaacaa cgaagcgata
     2581 atataattta tgataactcc gagtacatgc tgcatcaacc gcacagcgac ccaaagccct
     2641 atatagtttt ccgatgtgac aaaagcatac atgatatatg taggtccctg tagcagtaat
     2701 gcataagcga gtatcctgta ccgggaattc ttattaagag attttgtcag cgccttaccg
     2761 aaaaagatat gaatcagaaa gtataaggca tttatcaggt tgatttccca atagcttaaa
     2821 cctacact
//
LOCUS       DAOPWC010000182         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36952_length_2822_cov_2.443079, whole genome shotgun sequence.
ACCESSION   DAOPWC010000182 DAOPWC010000000
VERSION     DAOPWC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36952_length_2822_cov_2.443079"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKJ65_04670"
     CDS             complement(<1..634)
                     /locus_tag="PKJ65_04670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41162.1"
                     /translation="MKKLFQRLYIFLILLFLYAPIIVLIVYSFNASKSRAVWQGFSLT
                     WYQQLFTDSKILSALENTLLIALIASVVATVLGTFAAIGIMKMKKKTFETIRTITYIP
                     VLNPDIVTGVSLMMLFAFIGIELGFVSMLLAHITFCTPYVIISVLPKLYQLDMSVYEA
                     AQDLGAKPFYALRKTVIPQLMPGIVTGFLLSFTLSVDDFVISYFTTGGHVE"
     gene            complement(631..1458)
                     /locus_tag="PKJ65_04675"
     CDS             complement(631..1458)
                     /locus_tag="PKJ65_04675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41163.1"
                     /translation="MSKRRFSFPYLIWMTIFIVVPLFLIFFYAFTDNTSGKTLFTLEH
                     VKAIFQPLYLKVIGRSFYMSLIATLVCFVMGYPAAMILASKDFSQKNTMLILIVLPMW
                     MNFLLRTYSWLSLLENEGLINRFLASIGLGQVQLMYNNFAVILGMVYNFLPFMILPIY
                     SVLVKIDKSVVEAAKDLGANGARVFLRIIFPLSMPGVVSGITMTFMPALSTFVISRLL
                     SGGKITLIGNIIEQQFTITGDWGLGSALSLLLMLLIFISMIFINKYGDESVGAGGLL"
     gene            complement(1455..2534)
                     /locus_tag="PKJ65_04680"
     CDS             complement(1455..2534)
                     /locus_tag="PKJ65_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ41164.1"
                     /translation="MGKKIIELKNIYKRFGDFTAIDGINLYINEGEFLTLLGPSGCGK
                     TTTLRIIGGFEQPDEGDVLFEGVRINDVPAHKRPLNTVFQKYALFPHLTVGENVAFGP
                     TIRKISPDIIQKDVKKYLEIVNLEGYEDRDVSTLSGGQQQRVAIARALINKPRVLLLD
                     EPLGALDLKLRKDMQDELKTMHKDVGITFIYVTHDQEEALTLSDTVVVMNNGKILQIG
                     TPVDIYNEPNNAFVAYFIGESNIIHNSRMVKDKLVNIDGVDFNCVDSGFGQNAAVDVV
                     VRPEDVMLVPPDKAELKGVVTLVTFKGVHYEIWVEGPTFEWKVHSTIPQNIGDELGIT
                     VDPYNIHVMKHEPLEIPSAETAVEV"
     gene            complement(2554..>2822)
                     /locus_tag="PKJ65_04685"
     CDS             complement(2554..>2822)
                     /locus_tag="PKJ65_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ41165.1"
                     /translation="VVPDCQKNAMEPIIMTLLPGGVSYQDDPHEGEEFGYVLEGKIRL
                     EIDDKKYTVTRGQSFYFKADTKHLIRNNSDKKAVVLWVSTPPSF"
BASE COUNT          845 a          660 c          507 g          810 t
ORIGIN      
        1 tctccacatg tcccccggtt gtgaagtagc ttataacaaa gtcatccaca ctcaatgtaa
       61 aagataacag aaatcccgta actatacccg gcatgagctg aggtattacg gtttttctca
      121 gagcataaaa cggtttggca ccgagatcct gtgcagcttc ataaaccgac atatccaact
      181 gatacagttt gggtaatacc gagattatta cataaggcgt acagaatgtt atatgtgcca
      241 gcagcatgga cacaaacccc aattcaatac cgataaatgc aaacagcatc atcagagaaa
      301 cacccgtcac tatatcggga ttcaggacgg gaatatatgt tattgtcctt attgtttcaa
      361 aggttttttt cttcattttc attatgccga tagcggcaaa agtgcctaaa acagtggcaa
      421 caaccgaagc tataagagct ataagaagag tgttctccaa agcgctcagt atcttactgt
      481 cggtaaacag ctgttggtac catgttaaag aaaagccctg ccataccgca cgtgacttgg
      541 acgcattgaa tgaatatact atcagaacga taataggagc atacagaaac agcagtatca
      601 ggaaaatata taatctctgg aacagtttct tcataacagg cctcccgctc ccaccgactc
      661 atccccgtat ttatttataa atatcatgga gatgaatatc agaagcataa gaagcagtga
      721 taatgcagaa cccaatcccc agtcaccggt aatggtaaac tgctgctcaa ttatattacc
      781 tataagtgta attttacctc cgctcagcaa tcgggatata acgaaagtgg acaaagccgg
      841 cataaaagtc attgtgatac ccgaaacaac cccgggcata cttaacggaa atattatgcg
      901 caggaaaacc cttgcaccgt tggcacctaa atcttttgcc gcttctacta cactcttatc
      961 aatctttact aaaacggaat atataggcag aatcataaaa ggcagaaagt tatataccat
     1021 gcccaatatt acggcaaaat tgttatacat aagctgaacc tgccccaagc ctatagatgc
     1081 caggaatctg tttatgagac cttcattttc caacagtgac agccatgagt atgttctcaa
     1141 cagaaagttc atccacatag gtaacactat cagtataagc attgtgttct tttgggaaaa
     1201 atctttactt gcaagtatca tggctgcagg atatcccatt acaaagcata ccaaagtagc
     1261 tatcagtgac atataaaagg atcttcctat tactttcaga tacaaaggct ggaatatcgc
     1321 ctttacatgc tccaatgtaa agagtgtctt tccggaggta ttatctgtaa aggcatagaa
     1381 aaatattaaa aaaagcggaa cgacgataaa gattgtcatc cagataagat aaggaaaaga
     1441 aaaccgtctt ttactcatac ctccaccgcc gtttccgcag acgggatctc caacggttca
     1501 tgtttcataa catgaatatt gtaaggatcc acagttatac ccaactcatc accgatattc
     1561 tgaggtatcg ttgaatgaac tttccactcg aaggtcggcc cttccaccca aatttcgtag
     1621 tgtacgcctt tgaaagtgac caatgttacc acgcctttaa gttcggcttt gtcgggagga
     1681 acaagcatca cgtcctccgg tctcactaca acatccacag ccgcattttg tccgaatccc
     1741 gaatctacac agttaaaatc cacaccgtcg atatttacca atttatcttt aaccattcta
     1801 ctgttgtgga ttatattgct ttctcctatg aaatacgcta caaaagcatt attgggctcg
     1861 ttatatatat ctaccggagt tcctatctgc agtatctttc cgttattcat taccactacg
     1921 gtatccgaca aggtcaatgc ctcttcctga tcatgggtta catatatgaa ggtaattccg
     1981 acatctttat gcatggtctt aagttcgtcc tgcatatctt tgcgcagctt cagatccaaa
     2041 gctcccaaag gctcatccag aagcaatact ctgggtttgt ttataagggc gcgtgctatg
     2101 gccactcttt gttgctgacc gccgcttaag gtggagacat ccctgtcttc ataaccttca
     2161 agatttacta tttccagata ctttttcaca tccttctgta tgatatcggg actgattttt
     2221 ctaatagtgg gaccgaatgc gacattctcg cccaccgtaa gatgcggaaa taacgcatat
     2281 ttctgaaaaa cggtgttaag cggccttttg tgtgccggga catcatttat ccttacacct
     2341 tcaaaaagca catcaccttc atcaggttgt tcaaacccgc cgattattct caatgtggtg
     2401 gttttaccgc aacccgaagg acccaataat gttaaaaact caccttcatt gatgtataga
     2461 tttataccgt caattgcggt aaaatctccg aatcttttat aaatgttttt aagttcgata
     2521 atcttctttc ccatgtcttc tcccaagtta tatttaaaag ctcggcggtg tggacaccca
     2581 caaaaccact gctttcttat cggaattatt acgtattaaa tgtttagtat cggctttgaa
     2641 ataaaaggac tgtcctcttg tcaccgtata tttcttgtca tcaatctcca gtcttatttt
     2701 accttccagc acatatccga actcctcgcc ttcatgcggg tcatcctgat aacttactcc
     2761 gcccggtaac aaggtcatga tgataggctc catagcgttc ttctggcagt cgggtactac
     2821 cc
//
LOCUS       DAOPWC010000183         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_36977_length_2820_cov_4.090416, whole genome shotgun sequence.
ACCESSION   DAOPWC010000183 DAOPWC010000000
VERSION     DAOPWC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36977_length_2820_cov_4.090416"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..481
                     /gene="def"
                     /locus_tag="PKJ65_04690"
     CDS             2..481
                     /gene="def"
                     /locus_tag="PKJ65_04690"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ41166.1"
                     /translation="MRKEGDEILRKKSREVTSFDDRLHTLIDDMYETMVAAPGLGLAA
                     VQVGVLRRVAVIEIDDKKYELINPVIIQSEGEDIDVEGCLSLPDYYGTVKRPLKIKVL
                     YLDRYGKEQHAEVEGYAAHCFCHELDHMDGILFRDKVIEEIDPADLEEREKQSEEDK"
     gene            465..1415
                     /gene="fmt"
                     /locus_tag="PKJ65_04695"
     CDS             465..1415
                     /gene="fmt"
                     /locus_tag="PKJ65_04695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNZ41167.1"
                     /translation="MKKINNIIFMGTPDFALPCLIALIENGYTVNAVITQPDRPKGRH
                     NVVEYSNIKKKALKHGIPVLQPEKIRKKEWVETISKIGCDLAVTCAFGQILPQNILDI
                     PKYGTINVHASLLPKYRGASPMQRAIIEGEKITGVTTMLTDIGMDTGDMLLKMETDIT
                     EEDDICSLHDRLSHMGAKLLIDTVKLYEQDKITPVKQDNDKATYAPMIKKEEGRLDFN
                     LDSYRLFNLVRAFNPWPGTFTTYCGKTLKVLKADYSMEDYEEDVDNGTVISIDKERMA
                     VKCKKGILYITELQFENKKAMHISKCGHNMPCDMVLGCDE"
     gene            1463..2494
                     /gene="rlmN"
                     /locus_tag="PKJ65_04700"
     CDS             1463..2494
                     /gene="rlmN"
                     /locus_tag="PKJ65_04700"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ41168.1"
                     /translation="MSAPSIHILSYTHNELKDLFLEHGLKRYRALQVLDWIYNKKVFD
                     YSLMTNISKEDIEILQANFSLELPAIEKKLVSVDGTVKYLFRYSDNATVESVLMDYDY
                     GTSICISSQSGCKMGCVFCASSQVPFSRDLWGYEMTAQILKAAQDSNKTISRAVVMGI
                     GEPFDNYDNLVNFLNVINMKEGFNMGGRRITVSTCGIVPKIYEFADLCLQVNLSVSLH
                     EVTDDRRSAIMPINKKYNISSLIKACEYYFEKTNRRVSFEYALIPGSNDSLEHARHLS
                     ILLKNVMCYVNVIPVNPVENIKTVQNSKNLSPFCAILKAHGVPVTIRRELGRDIEAAC
                     GQLRRQHDI"
     gene            2484..>2820
                     /locus_tag="PKJ65_04705"
     CDS             2484..>2820
                     /locus_tag="PKJ65_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41169.1"
                     /translation="MIFNYASYTDIGQVRGNNEDYHIVIPFEEHNEFAAAVADGMGGH
                     LFGEIASKTAIDCFEKKIRDRRFKNCNNINDYLFNICNTANKEILDFSKKHNVFSGMG
                     TTLDAIYIKD"
BASE COUNT          933 a          479 c          643 g          765 t
ORIGIN      
        1 aatacgcaaa gaaggcgacg aaatattaag aaaaaagtcc agggaagtca catcttttga
       61 tgacagattg catactttaa ttgatgatat gtatgaaaca atggtagcgg cacccggatt
      121 gggactggct gcggtacagg tgggagtact cagaagggtt gccgttattg aaatagacga
      181 taagaagtat gagttgataa accccgtaat aatccaatct gaaggggaag atatcgatgt
      241 ggaaggatgc ttaagtctgc cggattatta cggaacggtc aagagacctt taaagattaa
      301 ggtgctctat cttgacaggt acggaaaaga acagcatgcc gaagttgagg gatatgcagc
      361 tcactgtttc tgtcatgaat tggaccatat ggacggtatt ttattcagag ataaagtaat
      421 agaggaaatt gatcctgcgg atttggaaga aagagaaaag cagagtgaag aagataaata
      481 atatcatttt tatgggtact ccggattttg cattgccttg tcttattgca ctgattgaaa
      541 acggatatac cgttaatgcc gttatcacgc agcctgacag acccaaaggc aggcataatg
      601 ttgtggagta ttcgaatata aagaagaagg cgcttaagca cggtatacct gttctgcaac
      661 cggagaaaat acgcaaaaaa gagtgggtgg aaaccatttc aaagataggt tgcgatttag
      721 ctgtgacctg tgcattcgga caaattcttc cgcaaaatat attagacata cctaaatacg
      781 gcaccattaa cgttcatgca tcgctgcttc ccaaatacag aggagcctct cccatgcaaa
      841 gagcgataat agaaggggag aagataaccg gtgtgacaac aatgctgacc gatataggaa
      901 tggataccgg agatatgtta ttaaaaatgg agaccgacat aacagaggaa gatgatatat
      961 gttctttaca tgacagactt tctcatatgg gagcaaaact ccttatagac accgtaaagc
     1021 tgtatgagca ggataagata acgcctgtta agcaggataa tgataaagcc acatatgctc
     1081 ccatgataaa aaaagaagag ggcagattgg actttaacct tgactcatac agactgttta
     1141 accttgtaag agcatttaat ccgtggccgg gaacatttac aacttactgc ggaaaaacct
     1201 taaaagtgct gaaggctgat tactctatgg aagattacga agaggatgtt gacaacggta
     1261 ctgttatatc catagataaa gagagaatgg ctgttaaatg caaaaagggc atattgtata
     1321 taacagagtt acagtttgag aataaaaagg caatgcatat atcaaaatgc ggtcacaata
     1381 tgccgtgtga catggttttg ggttgtgatg aataatgatg agtgcattga ttttcttttg
     1441 ataaaaatat ataatttccg acatgagcgc tccttctatt catatacttt cctatacaca
     1501 caacgaactt aaagaccttt ttttagaaca cggcttgaaa aggtacagag ctttgcaggt
     1561 tttagactgg atatataaca agaaggtatt tgattattcc ttaatgacca atataagtaa
     1621 agaagatatt gagattttgc aggcaaactt ttctttggag ttacctgcaa tagaaaagaa
     1681 gcttgtgtct gttgacggaa ctgttaaata tttattccgt tattccgata atgcaacggt
     1741 ggaaagtgtg cttatggact atgattacgg aacctccatc tgcatttcca gtcaatcggg
     1801 gtgcaagatg ggctgcgtat tctgtgcttc ttcccaagta cctttcagcc gggatttgtg
     1861 gggttatgag atgaccgctc agattcttaa ggcagcgcag gatagcaaca agaccatatc
     1921 cagagcggtg gtaatgggta taggagagcc gtttgataac tatgataatc ttgtgaattt
     1981 tctcaatgtt attaacatga aagaagggtt taacatggga ggaagacgca taaccgtttc
     2041 cacctgcgga atagtaccca agatatatga gttcgccgat ttgtgtcttc aggtcaatct
     2101 gtcggtatcg ctacatgaag ttaccgatga caggcgctcg gcaataatgc cgataaataa
     2161 aaaatacaac atctcttccc ttataaaagc atgtgaatac tattttgaaa agaccaacag
     2221 aagagtttca tttgaatatg ctctgatccc cgggtctaat gactccttag agcatgcgcg
     2281 acatttaagc attttattaa aaaatgtaat gtgctatgta aatgtaattc cggtaaatcc
     2341 tgttgaaaat ataaaaacag ttcaaaacag caaaaatctt tcgccttttt gtgctatact
     2401 gaaagcacat ggcgtgcccg tcaccatcag aagagaactg ggcagagaca ttgaagccgc
     2461 atgcgggcag ttaaggagac agcatgatat ttaattacgc ttcgtatacc gatatcggac
     2521 aggtaagagg aaataatgaa gattatcata tcgtcattcc ttttgaagag cataatgaat
     2581 ttgccgctgc ggttgccgac ggtatgggcg gtcatctgtt cggtgagata gccagtaaaa
     2641 ccgcaataga ctgttttgag aagaaaataa gggacagacg cttcaagaat tgtaacaata
     2701 tcaatgatta tctttttaat atctgtaata ctgccaataa ggagatactg gatttcagta
     2761 agaagcacaa tgtcttttcc ggaatgggta ccacattgga tgccatttac ataaaggatg
//
LOCUS       DAOPWC010000184         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37035_length_2817_cov_3.123099, whole genome shotgun sequence.
ACCESSION   DAOPWC010000184 DAOPWC010000000
VERSION     DAOPWC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37035_length_2817_cov_3.123099"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKJ65_04710"
     CDS             <1..854
                     /locus_tag="PKJ65_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41170.1"
                     /translation="SCAAVSVYDALQISDVCPYCGSNQVTEEKGADSLAPDGVCPFTV
                     TDKEAGANFHNWLKRKLFCPKAAKESAKPESFKGVYLPYWTFDTEADVKYTAEYGKEK
                     EVKDDNGETKKVMEWYPTRGTYNKFFDDYPVPATERYDQGTLNMIKPFDTENSIPYKP
                     EYVAGFISERYSVGLQKGWSIAKEDIKDILHSEITDKIEEENDADDVRKLDMKIKYND
                     IKYKYLLLPIWLSSFKYKEKIYRFMVNGRTGKVGGRSPVSPARVALAIGIVIAIILLI
                     LKLSGNI"
     gene            complement(965..1396)
                     /locus_tag="PKJ65_04715"
     CDS             complement(965..1396)
                     /locus_tag="PKJ65_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNZ41171.1"
                     /translation="MNQNLSDLEYALDNIVNVNGNYSGVEFEKSVADLLRMNGFLEVK
                     ITPYSGDYGVDIIAKYNNQKYVFQCKYYNNKNLGIKPIQEVFAGKTYYRADVAVVVTN
                     IYFSQNAQILAKKTNVQLWDRSHLSKLLRSARRNQILPWLK"
     gene            1651..>2817
                     /locus_tag="PKJ65_04720"
     CDS             1651..>2817
                     /locus_tag="PKJ65_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNZ41172.1"
                     /translation="MRNRFIMYILITVMLLTLCSCHDISDNGISPVNSPSTDPSPEVT
                     TATPSIEPSATEAVSTSTPGPEPTATPAPSQPPQRPIGYTDERYFRFDALHGIILDYD
                     MTGGIYVSIPETINGFKVTTIGEHAFQGRKLYGVVLPDSVTKISAYAFENCDISELDL
                     GKGVEYIGEYAFSTSVSYKDNTYSNPIWAEGLKTLALPDTVKHIGNNAFCNNSLTSIN
                     LPNGLEYLSGFNGNKLTHVEIPGSVKTVGDYAFSDNLLTSVTVPDSVVCIGVKAFQGL
                     TEDKVKLGAGSDRYFVVKGDSIVGHICTVEGTLTVPYYEGVSKIGFGAFWGQKGITKV
                     KISEGIKIIEDYAFRDTSLNRQKHSVWLPESIQFLPTVAFEGSPKFLFEYADGSF"
BASE COUNT          923 a          499 c          583 g          812 t
ORIGIN      
        1 aatcctgtgc tgctgtatcg gtatatgatg ctcttcagat atccgatgtg tgtccgtatt
       61 gcggttcaaa tcaggtgaca gaggaaaagg gagcggattc attggctccc gacggagtgt
      121 gtccttttac cgtaaccgat aaagaagcag gggcaaactt ccataactgg cttaagagaa
      181 aactgttctg tcccaaagct gcaaaagaaa gtgcaaaacc ggagtctttc aagggtgttt
      241 atctgccata ctggacattt gataccgaag cggatgtaaa gtacactgcc gagtacggaa
      301 aagaaaaaga agtaaaagac gacaatggcg aaaccaagaa ggttatggaa tggtatccga
      361 cacgcggaac atacaataaa ttctttgacg attatcccgt tccggcaacg gaaagatacg
      421 accagggaac tcttaacatg ataaaaccgt ttgacacgga aaacagcata ccttacaagc
      481 cggaatatgt tgcgggattc atatccgaaa gatactccgt aggtcttcaa aaaggatgga
      541 gtattgctaa ggaggatata aaagatatac ttcatagtga gataaccgat aaaattgaag
      601 aagaaaatga tgcggacgat gtaagaaaac tggatatgaa gataaaatac aatgatataa
      661 agtacaagta tctgctgctg ccgatatggc tttcatcatt caagtataaa gaaaagattt
      721 accggttcat ggtcaacgga agaacaggta aggtcggtgg acgttcacct gtttcaccgg
      781 cacgtgttgc cttggcaata ggtatagtaa ttgcaataat cctgctcatt cttaaactta
      841 gcggtaatat atagcgggaa aaacaaaaga ataacaaaca agaaacgtat agggagactt
      901 atacgttttt gatgtgtgaa agatagtgat taatacaaag taagagaacc atgcatagtc
      961 agttttactt aagccaaggt agtatctggt ttcttctagc agaacgaagc aattttgaaa
     1021 gatggcttct atcccataat tgaacattag ttttttttgc taaaatttgt gcattttgtg
     1081 aaaaataaat atttgttact actactgcaa catcagctct ataatatgtt ttacctgcaa
     1141 atacttcctg tatcggtttt attcccaagt ttttattatt ataatatttg cattgaaaaa
     1201 catatttttg gttattatat tttgcaatta tatcaacgcc ataatcaccg ctatatgggg
     1261 taatcttaac ctccaagaaa ccattcattc tgagtaagtc tgctacagac ttctcaaatt
     1321 caactcctga ataattgcca tttacattta caatattgtc aagagcgtat tctaaatcag
     1381 ataaattttg attcactcga cttcttatta tgaagaaagc agcaatgatt ataactaact
     1441 aatatatctc agtcaaactg tacataaaga agatagcact ttaagataat agcaaataat
     1501 atatgatatt atacaagcca tttcataagt ctatttgctt ttgtacaaca tatgaacttt
     1561 ttatgacata gttcactaaa tgtttacatc ttaaatgtaa atagtgagat acaatataga
     1621 aaagaaatat atgtgttgaa gagggacagg atgagaaata gatttattat gtatatcctt
     1681 ataactgtaa tgctgttgac attatgttca tgccatgata tttccgataa cggcatttcg
     1741 cccgtcaatt caccgagtac cgacccttcc ccggaagtta ctacagcaac tccgagtata
     1801 gagccttctg caactgaagc agtttctacg tctacacccg gtcccgagcc gactgctact
     1861 cctgcacctt cgcagccgcc gcaaaggcct atcggatata ccgatgaaag gtattttcgc
     1921 ttcgatgctc ttcacgggat tatattagac tacgatatga caggcggaat atatgtcagc
     1981 ataccggaga caataaacgg tttcaaagtg acaacaatag gagaacatgc ttttcaaggc
     2041 agaaaattat acggtgtggt attgcctgat tcagttacta aaataagtgc atatgctttt
     2101 gaaaattgtg atatatccga gctggatttg ggaaaaggtg ttgaatacat aggagaatat
     2161 gctttttcca cctcggtaag ctataaggac aatacataca gtaatccgat atgggcggaa
     2221 ggccttaaga cattagcttt acccgatacc gtcaagcata taggcaataa tgctttttgc
     2281 aataattcgc ttacaagtat taatttgccg aacggattgg agtatctttc gggatttaac
     2341 ggtaacaagt taacgcatgt ggagataccc ggcagtgtaa aaaccgtcgg cgattatgct
     2401 ttttcggaca atctgcttac gagtgttacc gttcccgaca gtgtagtatg catcggagta
     2461 aaagcctttc agggtcttac ggaagataag gtaaagctcg gggcaggatc ggacagatat
     2521 tttgttgtaa aaggtgacag catagtcgga catatttgta cggtagaagg aactcttacc
     2581 gtaccttact acgaaggagt cagtaaaata ggtttcggtg ctttttgggg tcaaaaggga
     2641 ataaccaagg tgaagatatc cgagggtata aaaatcattg aagattatgc attccgtgat
     2701 acttcgctaa accgacaaaa acattcggta tggttgccgg aaagtataca atttcttccg
     2761 actgtcgctt ttgaaggttc gcctaagttt ttatttgaat atgctgacgg ttcgttt
//
LOCUS       DAOPWC010000185         2816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37051_length_2816_cov_2.586020, whole genome shotgun sequence.
ACCESSION   DAOPWC010000185 DAOPWC010000000
VERSION     DAOPWC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37051_length_2816_cov_2.586020"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKJ65_04725"
     CDS             <1..1026
                     /locus_tag="PKJ65_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNZ41173.1"
                     /translation="LLTEKGSQRPYSNFGRYYDSKPYGGFYTQEEMKDIVEYCRKKSI
                     DVIPEIEIPGHSSACLHAYPHLSCTGKEVPVRTHAGIFKDILCAGKEEVYEFLFNVID
                     EMTAIFPGEYIHIGGDEAPKDRWKECPHCNKKMRELGLENYEQLQGYMLNRIYDHLQK
                     KGKQAITWNESLRGGNLEKGIIVQNWMEKNDECTIRASQGGKVIVSDFFHYYVNYPLG
                     MTPLKKTYMYEPIPSKLHENMKKNIIGVETPVWTEFIDNFEDMSKMCFPRMAAVAETG
                     WTNRDKKDYVDFKLRLTYVIRMIEEMGLKYSEESQWDPGFFQSAKQVIKFAYNMYNKE
                     TVKALRK"
     gene            1115..2563
                     /gene="gnd"
                     /locus_tag="PKJ65_04730"
     CDS             1115..2563
                     /gene="gnd"
                     /locus_tag="PKJ65_04730"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNZ41174.1"
                     /translation="MKKADIGLIGLAVMGENLVLNMESKGFTVAVYNRTVEKVEKFVQ
                     TRAKGKNIIGCYSLSELVDSLRKPRKVMIMVKAGAPVDNMIDQLIPLLDKNDIIIDGG
                     NSNFNDTIARTERVEKAGLMYIGTGVSGGEEGALTGPSLMPGGSESAWPHVKDIFQSI
                     AAKVEDGTPCCDWVGENGAGHFLKMVHNGIEYGDMELICEAYNIMKTLVGMDTDSMHN
                     VFAKWNEGELNSYLIEITADILAFKENKEPLVEKILDKAGQKGTGKWTAISALDEGVP
                     LTLITEAVFARCLSAMKDERVEASRILNGPTVSFNEKHDAFIEDLRKALYLSKIVSYA
                     QGYALMKAAAASYDWHLNYGGIALMWRGGCIIRSAFLGKIKQAYDNNPEISNLLLDPF
                     FKQKADECHQSLRKIVSVCALNGIPIPAFAAALTYYDGYRSKRLPANLLQAQRDYFGA
                     HTYERIDSERGVFYHTNWTGRGGETSASTYNA"
     gene            2575..>2816
                     /locus_tag="PKJ65_04735"
     CDS             2575..>2816
                     /locus_tag="PKJ65_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ41175.1"
                     /translation="MKLPFKINLKNKVVVVTGAGGVLCSMFAEALAEAGAKVALLDLN
                     YDAAKKVSDEINGKGGITIPVKANVLEKQSLLETIKA"
BASE COUNT          930 a          482 c          703 g          701 t
ORIGIN      
        1 cttcttaccg aaaaaggttc gcaaagaccg tattcaaatt tcggaaggta ttatgattct
       61 aagccgtatg gcgggttcta tactcaggaa gagatgaagg atattgtgga gtattgcaga
      121 aaaaagagta ttgatgtgat accggaaata gagatacccg gtcattcgtc agcctgcctt
      181 cacgcatatc cccacctgtc ctgtacaggt aaggaagtac cggtacgcac tcatgcgggc
      241 atattcaagg atatcttatg tgcgggaaaa gaagaggtat atgagttttt atttaatgtc
      301 atagatgaaa tgactgctat tttcccgggt gaatatatac atatcggcgg agatgaagcc
      361 cccaaggaca gatggaaaga atgtccgcat tgcaataaaa agatgaggga gctcgggttg
      421 gagaattacg agcagctgca gggatatatg ctgaacagga tatatgacca tctgcaaaag
      481 aaaggtaaac aagccataac atggaacgag agcttacgcg gcggtaatct ggaaaaaggt
      541 ataattgttc agaattggat ggaaaagaat gatgagtgta caataagagc ttcacaaggc
      601 ggtaaagtaa ttgtatcgga tttcttccat tactatgtta attatccttt ggggatgaca
      661 cctcttaaga aaacatacat gtatgaacct ataccttcca agcttcatga gaatatgaaa
      721 aaaaatatca tcggagtgga gacaccggta tggacggagt ttattgataa tttcgaggat
      781 atgagtaaaa tgtgtttccc gcgaatggct gcagtagcgg aaacaggctg gacaaacagg
      841 gataaaaagg attatgtcga ttttaaactc agacttactt acgtgatacg aatgatagaa
      901 gagatgggat taaaatattc agaagaatcg cagtgggatc cgggcttttt ccaatcggct
      961 aaacaggtaa tcaaatttgc atacaatatg tacaacaaag agactgtgaa agcacttaga
     1021 aagtaagcgc atcaatggac aaaaaggcgg ttgtattata aaatacatag ttgaatacgg
     1081 tatgttatat acctgaaaac agaaagggtt agatatgaaa aaagctgata tcggccttat
     1141 aggccttgcc gtaatgggcg aaaatttggt gctcaacatg gagagtaagg gatttaccgt
     1201 ggctgtgtac aacagaacgg tggaaaaggt ggaaaaattc gttcagacaa gagcgaaagg
     1261 taaaaacatt ataggttgtt acagtctttc tgagcttgta gacagtttga gaaaacccag
     1321 aaaagttatg attatggtaa aagccggtgc accggtggac aatatgatag atcaactgat
     1381 acctcttctg gataagaatg acattataat tgacggcgga aattccaatt tcaacgatac
     1441 catagcaaga accgaaagag tggaaaaagc cggacttatg tatatcggta cgggagtgag
     1501 cggcggggaa gaaggcgccc tgacaggtcc ttctttgatg ccgggaggca gtgaaagcgc
     1561 atggcctcat gtaaaggata ttttccagtc catagccgcc aaagtggaag acggcacacc
     1621 ctgttgcgat tgggtaggtg aaaacggagc cggtcatttt ttaaagatgg ttcataacgg
     1681 tattgaatac ggtgacatgg aacttatctg cgaggcatat aacataatga agactcttgt
     1741 aggtatggat accgacagta tgcataatgt ttttgccaaa tggaatgaag gggaactgaa
     1801 ttcctatctt atagagataa ctgcggatat actggcattt aaggaaaaca aagagccgtt
     1861 ggtggaaaag attttagata aagcgggaca gaagggtaca ggtaaatgga cagcgatttc
     1921 cgcattggac gaaggcgtac cccttaccct cataaccgaa gcggtgtttg caagatgctt
     1981 atccgctatg aaagatgaaa gggttgaagc aagccgcatt cttaacggtc ctaccgtgtc
     2041 gtttaatgaa aaacatgatg cttttataga agatttgaga aaagctcttt atctctccaa
     2101 gatagtgtct tacgcacagg gttacgcatt gatgaaagcc gctgcagcat cttatgactg
     2161 gcaccttaat tacggaggca tagcccttat gtggagaggc ggatgcatta tccgttcggc
     2221 attcttgggt aagataaagc aggcatatga taacaatccc gaaatatcca atcttttgtt
     2281 ggatcctttc ttcaaacaaa aggccgatga gtgtcatcaa agtttaagga agatagtatc
     2341 ggtatgtgct cttaacggca ttcccatacc ggcatttgcc gccgcactga catattacga
     2401 cggttacaga agcaaaagat tgccggcgaa tttattacag gcacaaagag actatttcgg
     2461 tgcacataca tatgaaagaa tcgacagtga aagaggagta ttctatcaca caaactggac
     2521 gggaaggggc ggagagacat ccgcatctac atataacgcg taaggaggcg ggaaatgaaa
     2581 ttaccattca agattaattt aaaaaataag gttgttgtgg ttacaggtgc cggaggagta
     2641 ttatgttcca tgtttgcaga agctcttgcc gaagcaggtg caaaagttgc acttttggac
     2701 cttaattatg atgcagccaa aaaagtttcc gacgagatta acggtaaagg cggaataacc
     2761 atacctgtta aagccaatgt actggaaaaa cagagtcttc tggagacgat caaggc
//
LOCUS       DAOPWC010000186         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37230_length_2806_cov_3.103599, whole genome shotgun sequence.
ACCESSION   DAOPWC010000186 DAOPWC010000000
VERSION     DAOPWC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37230_length_2806_cov_3.103599"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKJ65_04740"
     CDS             complement(<1..237)
                     /locus_tag="PKJ65_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41176.1"
                     /translation="MGKSVYSLILNDEVIKKIDMLAYARRTSRSNYISEVLASHVSYT
                     TPQQRIKDILDAARAFLEPYEKYAFVEMNSNSFMD"
     gene            460..1641
                     /locus_tag="PKJ65_04745"
     CDS             460..1641
                     /locus_tag="PKJ65_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HNZ41177.1"
                     /translation="MKKPVKNNVYWVGKTDWELKKFHGEELSTHHGSSYNSYLIMEEK
                     TVLIDTVWSPFENEFVENLEKEIDLKKIDFIVCNHGEVDHSGALNKLMEKIPSTPIYC
                     SANAVKSLQGQYFKNWDLRPLKTGDTIDIGNGKQLVFVEMRMLHWPDSMACYLTKDNI
                     LFSNDAFGQHYACDHLFNDSADQCTLYAEAEKYYANILAPFSAILKRKLEEIHSLNLD
                     IDIIAPSHGVIWRDNPMQIVEKYRLWCDNYQENLITIVYDTMWNGTRALAEKIAEGIR
                     EKDESVNIRIFNLPKSDMNDVATQIFKSRTVLLGSPTVNNCALHSLAALTHYIKGLKL
                     KNKKAAAFGCYGWSGESVKILNEMLTDAGFEVVNEGFRCLWNPDYETLQKAADFGKDI
                     A"
     gene            1771..2331
                     /locus_tag="PKJ65_04750"
     CDS             1771..2331
                     /locus_tag="PKJ65_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41178.1"
                     /translation="MAFFDKLKKQATSAIEKALDPRHKTQKITFADLPETLEAFKALP
                     EAQLASPFETAALTVVAFCLYPVNKELCHQMLNYLRGPRPMSEMDKQFIRDRFLDKDY
                     VPRSYFDGATPANDYMPSKPYTITVTSNPRSFDDPDTVRLYINSGGADSPRLVSLRKA
                     KDGKWYLWEQYLLADIRKPESENPWA"
     gene            2407..2802
                     /locus_tag="PKJ65_04755"
     CDS             2407..2802
                     /locus_tag="PKJ65_04755"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNZ41179.1"
                     /translation="MKIIVKDNYSEMSKLAASVIADLVRKKPDCVLGLATGATPIGTY
                     HELARLHKEEGLDFSAVTTFNLDEYLGISMDLTLPYEMDQSYARFMHEELFKKINIKK
                     KNAHVPYGLAADPDKYCKEYDLVKSNSKI"
BASE COUNT          897 a          545 c          610 g          754 t
ORIGIN      
        1 atccataaag gaattggagt tcatctccac gaaagcatat ttttcgtacg gttccaaaaa
       61 agctcttgcg gcatctaaaa tgtcttttat cctctgctga ggagtggtat aggacacatg
      121 tgaagccagc acctcactta tatagttgga acggctggtt cgccttgcat atgccagcat
      181 atcaatcttt tttattacct catcgttaag tatgaggctg tatacggact ttcccatcac
      241 gtacctctct ttcagaatta ttgtatcact tgttcagtaa tttggcaatg atattatata
      301 atcttattaa caaatgtttt ccttcaacaa atacaagaaa ggttacggtg acatactcac
      361 ttaaactgtt tattgtgtat gatataattt atttaaataa gcatttatta atttaagtat
      421 tgaaatgata tatacaatat ctgcaagaaa gggattttta tgaagaagcc ggtaaagaat
      481 aacgtatact gggtaggaaa gaccgattgg gaactgaaga aatttcacgg tgaagagttg
      541 tccactcatc acgggtcaag ttataacagt tatttaataa tggaggaaaa gaccgtcctc
      601 atagataccg tatggtctcc gtttgaaaat gaatttgtgg aaaatctgga aaaggaaatt
      661 gacttaaaga agattgattt catagtctgc aatcacggtg aagtggacca cagcggagca
      721 cttaacaagt tgatggagaa aataccctcc actcccatat actgctccgc caatgccgta
      781 aaatcgttac agggacaata ttttaagaac tgggatttgc gtcctttaaa aaccggagat
      841 accatagaca taggtaacgg caagcagttg gtctttgtgg aaatgcgtat gctgcactgg
      901 ccggacagta tggcctgcta cctgacaaaa gacaatatac tgttttctaa tgatgcattc
      961 ggtcagcact atgcttgcga ccatctcttt aacgattctg cagatcaatg cacactgtat
     1021 gccgaagcgg aaaaatatta cgcaaacatt ctggctccct tcagtgcgat actcaaaaga
     1081 aaactggagg aaatacattc acttaatctg gatatagata ttattgcccc cagtcacggt
     1141 gtgatatgga gagacaatcc tatgcagata gtggaaaaat acaggttatg gtgtgacaac
     1201 tatcaggaaa acctcataac cattgtttac gataccatgt ggaacggaac cagagcgctt
     1261 gccgaaaaga tagcagaggg tataagggaa aaagatgaga gtgtaaatat acgcatattc
     1321 aatcttccca agagcgacat gaatgatgtt gccacacaga ttttcaaatc caggaccgtt
     1381 cttttaggtt ctcctacagt caataactgt gcactgcatt cgcttgccgc actcacccac
     1441 tacattaaag gtctgaagct gaagaacaag aaggctgcgg cattcggctg ttacggttgg
     1501 tcgggagaat cggttaagat attgaacgaa atgcttaccg atgcgggatt tgaagtggtt
     1561 aatgaaggat tcagatgcct ttggaatccc gattacgaaa cactgcaaaa agcggcggat
     1621 ttcggtaagg atattgcgtg atttagcttg tgaatattga aaaacgagac aaattttgat
     1681 atcctataat aaggaagttg tcggtgtaag acagtatgcc gatatccttc cgtcagtaca
     1741 gaagtataaa aaaagtaaaa ggagagaatc atggcatttt ttgacaaatt gaagaaacag
     1801 gctacatcag cgatagaaaa agctctcgat ccgagacaca aaactcagaa gattactttt
     1861 gccgatctgc ctgaaacatt ggaagcattt aaagcactcc ccgaggcgca gcttgcatca
     1921 ccgtttgaga cggcggcact taccgttgtg gcattctgcc tctatcccgt aaataaggaa
     1981 ttgtgccatc agatgctcaa ttatttacgc ggaccgcgcc cgatgagcga aatggataag
     2041 cagtttataa gagaccgctt tttggataaa gattatgttc cgcgttctta cttcgacggt
     2101 gcaacacctg ccaacgacta tatgccgtca aagccctata caataacggt tacttccaat
     2161 ccccgttcat tcgacgatcc ggataccgtt agactgtata ttaattcggg cggagccgac
     2221 agtccgcgtc tggtatcgct tcgtaaggca aaggacggaa aatggtatct ttgggaacag
     2281 tatctccttg ccgatatacg taaaccggaa agcgaaaatc cctgggctta attgtttctg
     2341 aagagatatt ttcagtatac cgtcaagaac agatgaataa aattaaataa aagaaagaag
     2401 gatgctatga agattattgt taaggataat tatagcgaga tgagcaaact tgcagcttcg
     2461 gttattgccg atctggtaag aaagaaaccc gactgcgtat tgggactggc aaccggcgct
     2521 acacctatcg gaacctatca tgaacttgcc aggctgcata aagaggaagg tctggacttt
     2581 tctgcagtca caacattcaa tctggatgaa tatctgggaa tatccatgga tcttacgctt
     2641 ccgtatgaga tggaccagtc ctatgctcgg ttcatgcatg aagagttgtt taagaagata
     2701 aacatcaaaa agaaaaatgc tcatgtaccg tacgggcttg cagccgatcc ggataaatac
     2761 tgcaaagaat atgacctggt taaatcaaac agcaaaattt gaaata
//
LOCUS       DAOPWC010000187         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37290_length_2803_cov_3.008006, whole genome shotgun sequence.
ACCESSION   DAOPWC010000187 DAOPWC010000000
VERSION     DAOPWC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37290_length_2803_cov_3.008006"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKJ65_04760"
     CDS             <1..596
                     /locus_tag="PKJ65_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41180.1"
                     /translation="KLFGVYSDIYRVIIAQNRDLTFSDQYNIYTIHTDGSVTYRYASA
                     SQIQEKGEISLAFNNALSMLGDLTSLSAAEKNPPVLAFVGIEEDCYEFRFVYRYNGYP
                     VLLDKEKYCISITATADRVVAADVNLFDISDASLPTVMYDLRTFNMLSENDMALGSIK
                     ANGMYIAYMKDDSTVLLPKWIIEGEEITELGLKEVKH"
     gene            596..1369
                     /locus_tag="PKJ65_04765"
     CDS             596..1369
                     /locus_tag="PKJ65_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41181.1"
                     /translation="MDSKKALRIIIVLLLAVNIFMAAFIINLVYSEPDTKDEHKYITE
                     ILAYRDITLDCEIPQYAAPSAVIVKSDSDNSALLSYLEAQGGTISDSQEDIIIYTPAV
                     KQRYEDLTLETAAKIADDFIKKLPVDNQSYMPDSILTAGVGVYKVNYIYLDGSYCIYD
                     KKIEMTISKNGIDRVYIGCPEYTVAETEYNRPMVSVGNILVSGFTRQGRSFITIKKID
                     AGYIYDKNTSSFVNVWRVMYNNGQERYFSSVDGTVIVAD"
     gene            1563..>2803
                     /locus_tag="PKJ65_04770"
     CDS             1563..>2803
                     /locus_tag="PKJ65_04770"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ41182.1"
                     /translation="MKYYESNRLELKEKLNDKFIRAVVAFLNTDGGRVLIGVTDDGTV
                     VGVNEVDKTLKQIADIITAQIEPSPTDSVKTEILMEEGKVLVSVSVLKGIKPIYCIKK
                     YGFSSVGCPIRVGSTCREMTEEQIANRYKLKFLNNDLITEAVTNLPLLSFRSLKQYYL
                     DKGYRLNEDNFETNLYLIAPSGKYNIMGELLSDNNRYSLIYVKFRGLDKASISQRTDY
                     GQRSLIFAYEQLMNRIASENICTTDTTIRPRKDTYLYEYDCVKEAVINAIVHNDWLIS
                     EPQVSFYTDRLEVFSHGGLLHGLTKEEFFRGISKPRNLRLMKIFSDLDIVEHTGHGVP
                     TIIRKYGKEVFDITDNHIMVTIPFERSVLNEVIQNNVGINVATNVDINKTEKDIMEII
                     LREPRATYGVIAEKIEKTPKTV"
BASE COUNT          906 a          487 c          600 g          810 t
ORIGIN      
        1 ccaaattgtt cggtgtttat tccgatatat atagggtaat aattgcacag aacagggatt
       61 tgactttttc cgaccagtac aatatctaca ctattcatac cgacggctcg gtcacttaca
      121 gatatgcttc ggcatcacag atacaggaaa aaggagagat ttctttagct ttcaataatg
      181 ctttaagtat gcttggcgat cttacttcgt tatcggcagc cgagaaaaat ccgcccgtgt
      241 tggcttttgt cggaatagag gaagattgct atgagttccg ttttgtatac cgctacaacg
      301 gttatcccgt cctgctggat aaggagaagt attgtataag cataaccgca acggcggaca
      361 gagtggtggc tgccgatgta aatcttttcg atatttccga tgcctcatta cctacagtta
      421 tgtacgactt acgtacattt aatatgcttt cggaaaatga tatggcttta ggctccataa
      481 aagcaaacgg catgtatatt gcatacatga aagatgacag taccgtgctg ctgcctaaat
      541 ggataattga gggagaggaa ataacggaac tcggactgaa ggaggttaag cactgatgga
      601 ttctaaaaaa gctctcagga taataatcgt gcttctgctt gcagtcaata tattcatggc
      661 cgcctttatt atcaatcttg tctattccga accggataca aaagatgagc ataagtatat
      721 aaccgagata cttgcataca gggatatcac tttggattgt gaaatacccc aatatgcagc
      781 accgagtgcc gtaattgtaa aatccgattc ggataacagt gcgcttttgt cttatctgga
      841 agcgcagggc ggtaccatat ccgattcaca ggaagacata atcatatata ctcctgcggt
      901 taagcaaaga tatgaggatc ttacactgga aaccgctgca aagatagcgg atgatttcat
      961 taagaaactg ccggtagaca accaatcata catgcccgat tccatactga cagccggtgt
     1021 cggggtatat aaggtaaatt atatatatct tgacggttca tactgtattt acgataagaa
     1081 gatagaaatg acgataagta agaacggaat agaccgtgta tatatcggat gtcccgaata
     1141 tactgtagcg gagacggaat ataacagacc tatggtttcc gtaggcaata tattggttag
     1201 cgggtttacc cgtcagggtc gttctttcat caccataaag aaaatagatg cgggatatat
     1261 atatgataag aacacatcgt cttttgtcaa tgtgtggcga gtgatgtata acaacgggca
     1321 ggaaagatat ttctcatcgg ttgacgggac ggttattgtt gcagattaat caaatccctt
     1381 aataatcatg tcaatatcca tgtttctacg taaatccaag cctaccgatg aagcaattaa
     1441 tataccacct ttcaaaatga aattcttcct atatgaaatg atttgataat gtcgctataa
     1501 atgtcggtat atattgacat aatgtcgcat atagatatat aatcattatg aggtgatatc
     1561 ttatgaaata ttatgaatcc aaccggcttg aattgaaaga gaaattgaac gataaattta
     1621 tccgtgcggt tgttgcgttt ttgaataccg atggcggcag agtacttatc ggtgtaactg
     1681 atgacggaac agtagtggga gttaatgagg ttgataagac cttaaaacag attgcagata
     1741 ttattaccgc tcaaattgag ccgtctccga cagatagcgt gaaaaccgaa attcttatgg
     1801 aagaaggtaa ggtgcttgtt tccgtttcgg ttttgaaggg aataaagcct atatactgca
     1861 tcaaaaaata cggtttttcc tccgtaggtt gtcccattcg tgtcggatct acctgtcgtg
     1921 aaatgactga agaacagatt gcaaatcgtt acaagttgaa gtttctgaat aatgatttaa
     1981 taacggaagc ggttaccaac ctgcctttac tttcttttcg ttcactcaag cagtattacc
     2041 ttgataaagg atataggctc aacgaggata actttgagac aaacctgtat ttaatagcac
     2101 cgtcaggaaa atacaatatt atgggtgagc ttttgtcaga caataatagg tattctctaa
     2161 tatatgtgaa attcagagga ttggataaag catctatttc acagcgaacc gattacggac
     2221 agcgttcatt gatatttgcg tatgaacagc ttatgaaccg tattgcttcg gaaaatattt
     2281 gcactaccga tacaacaata agaccgagaa aagataccta tctttatgag tatgactgtg
     2341 taaaagaagc agttatcaat gctattgttc acaacgactg gttgatcagc gaaccgcaag
     2401 tatcttttta taccgacaga ttggaagttt tttcccatgg cggattactg cacgggttaa
     2461 cgaaagaaga atttttccgc ggaataagca aaccgagaaa tttaagattg atgaaaatat
     2521 tttccgattt ggatattgtg gagcatacag ggcacggtgt cccgacaata atcaggaaat
     2581 acggaaagga agtctttgat attaccgata atcatattat ggttacaatt ccctttgaaa
     2641 gaagtgtttt aaatgaagtt attcagaata atgtcggaat aaatgtcgct acaaatgtcg
     2701 atataaataa aactgaaaaa gatattatgg aaataatatt gagagaaccg agggctacat
     2761 acggtgttat tgccgagaag atagaaaaaa cacctaagac ggt
//
LOCUS       DAOPWC010000188         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37339_length_2800_cov_1.935155, whole genome shotgun sequence.
ACCESSION   DAOPWC010000188 DAOPWC010000000
VERSION     DAOPWC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37339_length_2800_cov_1.935155"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKJ65_04775"
     CDS             <1..667
                     /locus_tag="PKJ65_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNZ41183.1"
                     /translation="LDYICMMRKKKNIMPKCPVAIKTIVTTELATKIAEHYGVTVTDT
                     LTGFKFIGDQIAILEEKGRKDSLIFGFEESCGYLSGTHVRDKDAVNAAVLIADMADYY
                     NDKDISLLDRLEELYKQFGYLISYLGNYEYPGTAGLSKMRSIMENLRKSALLTKDYLN
                     ETDMLHADVLKFVIEDLGTAIVRPSGTEPKIKIYYFAEGCDMIDAKEKMKKLTVLIER
                     MLN"
     gene            673..1326
                     /locus_tag="PKJ65_04780"
     CDS             673..1326
                     /locus_tag="PKJ65_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41184.1"
                     /translation="MRKMTYISSHKRKKRFNYEIFGLLYCLALIGIIIYYHLTKDISI
                     SAFKADLFSPEYHTVLKPFYFILIFCGLLLLIFGFGFVGMLRTRVAYLLYPASVLTCI
                     FLSLSGFLYELHDRFHFFNACLISASVLFSVLILCFIIEKPYSSKYASLTMRIIDIVC
                     GSLFIAYTIWKISGILRDFLLSYEGYFQLIMLSVSVVFVMSNAVYIYLKFETRYSVK"
     gene            complement(1332..1877)
                     /locus_tag="PKJ65_04785"
     CDS             complement(1332..1877)
                     /locus_tag="PKJ65_04785"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNZ41185.1"
                     /translation="MQNLTFTDKQSLRKYMTGKREAMSPEQVRLYSKNLFDKFLLSDL
                     TTFESYMVYIPIKNETDTYLLIDYLLNTGKQVSVPYIDKKNKLVPVLIEKNTVYEKDT
                     FGINIPEQIQIVSPDLLDVIFVPGVAFDISGNRVGYGRGYYDAFLKDLHCITCAWCYD
                     FQIIDKIPDVSPADVRIKRFL"
     gene            complement(1887..>2800)
                     /locus_tag="PKJ65_04790"
     CDS             complement(1887..>2800)
                     /locus_tag="PKJ65_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNZ41186.1"
                     /translation="TTMQHIFMGLDPTGIGVAPFTPVTLDTHIFDTDAPELKWDVKIN
                     KKGKIVVCASIASYVGADILTGILATGIHKADKPCILLDIGTNGEIVLGSKDGIFSCA
                     TAAGPAFEGANISCGVAGIEGAINTVSYDDANKFTTIGNKKPIGICGSGLIDAVYSML
                     KNGIIEESGYMEAEEFKITDNVILTQKDIREVQNAKAAIAAGLKILIRRSGYKYSEID
                     KLYLAGGFGTVMNVESSTGIGLIPAELKDKVIPAGNTSLAGTSMLLLDKANIDTIDSI
                     RKMTQYIELSQDNEFTEEYVDNMFFEV"
BASE COUNT          820 a          549 c          516 g          915 t
ORIGIN      
        1 attagactat atatgcatga tgagaaaaaa gaagaatatt atgccaaaat gccccgttgc
       61 tattaaaacc atagtaacaa cggaactggc aacaaaaata gccgaacatt acggagtaac
      121 agtaacagat actcttacgg gatttaaatt catcggggat cagatagcaa tccttgaaga
      181 aaaaggtcgg aaagacagct taattttcgg atttgaagaa agctgcggat atctttccgg
      241 aactcatgtc cgggataaag atgccgttaa tgcagccgtt ctgatagccg acatggcgga
      301 ttattacaac gataaggata tttcccttct tgacagactg gaagagttat ataaacagtt
      361 cggatatctc atatcctatc tgggcaatta cgaatatccg ggaacggcgg gattgagcaa
      421 gatgagaagt ataatggaaa acttacggaa aagtgctctg ttgacaaaag attatctcaa
      481 tgaaaccgat atgcttcatg ccgatgtgct taaatttgtg atagaagatt taggaacagc
      541 tatcgtacgc ccctccggca ccgagcctaa gattaaaata tactatttcg cagaaggctg
      601 tgatatgata gatgcgaaag aaaaaatgaa aaaactgact gtactgattg aaaggatgtt
      661 gaattagtat ctatgcgtaa aatgacatac atcagttcgc ataaacggaa aaagcgattt
      721 aattacgaga tattcggatt actctattgt ttggcgctta taggtatcat tatctattat
      781 catctgacaa aagatatatc tatatccgca tttaaagccg atctgttttc ccctgagtat
      841 catactgttc tgaagccttt ttatttcatt cttatctttt gcggtctttt acttctgata
      901 ttcggcttcg gatttgtggg tatgctcagg acgcgtgtag cttatttgct ttatcccgca
      961 tctgttttaa cctgcatttt tctgtcatta agcggttttt tgtatgaact gcatgacaga
     1021 ttccattttt tcaacgcttg tcttatttcg gcttccgtct tattttccgt acttatattg
     1081 tgtttcataa tagaaaagcc ctattcatca aaatatgcaa gtttaaccat gagaataata
     1141 gatattgtct gcggttcact ttttattgca tatacgattt ggaaaatatc aggcatacta
     1201 agagattttc tgttatcata tgaaggatat tttcaactta ttatgttatc ggtttccgtt
     1261 gtttttgtca tgagcaatgc tgtttacata taccttaagt ttgagacacg atattccgtc
     1321 aagtagtagg attataaaaa cctttttatt ctgacatcag ccggtgagac atcgggtatt
     1381 ttatctataa tctgaaaatc gtagcaccag gcacaggtta tacagtgaag gtcttttaaa
     1441 aaggcgtcat aatatcctct gccgtaccct actctgttac ctgaaatatc aaaagctaca
     1501 ccgggaacaa atatgacatc caaaagatcg ggagatacaa tctgaatttg ttcgggtata
     1561 ttgataccga aagtatcttt ttcataaacc gtatttttct caatcaatac gggaaccagt
     1621 ttatttttct tatcaatata aggaaccgat acctgtttac cggtatttaa aagataatca
     1681 ataagcaaat atgtgtctgt ctcatttttt atgggtatat ataccatata gctttcaaat
     1741 gttgttaaat cggataagag aaatttatca aaaagattct ttgagtaaag ccgtacctgt
     1801 tccggtgaca tagcctctct tttcccggtc atatatttcc ttagggattg tttgtctgta
     1861 aaagttaagt tctgcaattt atgttctcaa acctcaaaaa acatgttgtc cacatactcc
     1921 tcggtaaact cgttatcctg actgagttct atgtattgtg tcattttacg tatggaatct
     1981 attgtgtcga tatttgcctt atccagaaga agcatggacg taccggctaa agatgtgtta
     2041 cccgcaggta ttactttatc tttaagttcg gcaggaataa gacctattcc ggtggaactt
     2101 tccacattca taacggttcc gaaacctccg gccagatata atttatctat ttcggaatat
     2161 ttatatcctg atcttcttat gagtattttc aatcccgcgg cgatggcggc ctttgcattc
     2221 tgcacttccc tgatatcttt ttgtgtcagt attacattgt cggtaatctt gaattcctcc
     2281 gcttccatat aacccgattc ctcgattata ccgtttttaa gcatggaata tacggcgtca
     2341 attagtcccg aaccgcatat gccgataggc tttttattac ctatcgtagt gaatttgttt
     2401 gcatcatcat agcttacggt attgattgca ccctcaatcc ccgcaacacc gcaggaaata
     2461 ttggctcctt caaatgccgg acctgcagcc gtggcacagc tgaatattcc atcctttgaa
     2521 cctaatacaa tctcaccgtt tgtgcctata tccagtaata tacacggttt atcagcttta
     2581 tgtatacctg ttgccagtat gcctgtcaga atatccgcac ccacataact tgcaatactt
     2641 gcacaaacaa caatctttcc ctttttattt atctttacat cccacttgag ttcgggagca
     2701 tcggtatcga atatatgagt atccagagtg accggagtaa acggtgcaac tccgatacct
     2761 gtcggatcca atcccatgaa aatatgctgc atggtggtat
//
LOCUS       DAOPWC010000189         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37379_length_2797_cov_3.103574, whole genome shotgun sequence.
ACCESSION   DAOPWC010000189 DAOPWC010000000
VERSION     DAOPWC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37379_length_2797_cov_3.103574"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKJ65_04795"
     CDS             complement(<1..275)
                     /locus_tag="PKJ65_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41187.1"
                     /translation="MIVKVLLLSFLVIAFTVFFFYSRLLFMNIESDYSNENRKEVRLT
                     GYVTEIVKDQVNKFKVILKCNNGFNYFVTMKDDCIRIGDIIELSGML"
     gene            complement(531..2201)
                     /locus_tag="PKJ65_04800"
     CDS             complement(531..2201)
                     /locus_tag="PKJ65_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNZ41188.1"
                     /translation="MNNYIVKCVSSLEKCFLDDNIQDKKQYTSFSCLLNEKLNFQIAY
                     TMTSDTLKDKSPCYFHIQSELKDFIRVYKVESVAVRLPVYHNLPINNYQRKVPHCYPD
                     LLCPYDKKDNVYFVPNLLQSLFIEVDLSDNTCFYGDYVIKCYFTDNDGNTVASTDVSV
                     NIINSMLSDKKITFTQWFHGDCLASYYAVKPLSKKHFTIMKNFLKTAYEYGINAVLTP
                     VLTPPLDTKIGGERPTMQLVDIKKTSSGYEYSYDLLDKWIDMCNEIGFEYFEISHFFT
                     QWGAKHAPKVVAYVNGRKKRIFGWETDATSDEYISFLRDFISNLLIYLKNKGVDKRCF
                     FHISDEPNEKNIEGYRNARNSIIDLLDGYLVTDALSSIEFYKQGIVTSPIPATDKIEP
                     FLKQNIDNLWTYYCCGQFIDVSNRFIAMPSQRNRIIGTQLYKYGIKGFLQWGYNFYYN
                     QYSRKLVNPFLITDGDFFAPAGDAYSVYPGHDQKPLKSLRLVVFNEALQDLRAFLKLE
                     SLTSYEHVMKIIESNGKISFSKFPEDIEYIINLRERVNKEIGKLTKEK"
     gene            complement(2198..>2797)
                     /locus_tag="PKJ65_04805"
     CDS             complement(2198..>2797)
                     /locus_tag="PKJ65_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNZ41189.1"
                     /translation="EMSVKRDVAGRVSSVSDEINFLYQRKADCGVILENIKSGKIPLR
                     VTHNDTKLNNLLFDNETDKPICILDLDTVMPGSILYDYGDALRISGSSAEEDEENLDK
                     VHFVTDNFIYFTKGFLAAVKNSINAVELSLLPFSIKLITMEIGMRFLTDYLNNDIYFA
                     VHKENHNLIRARTQFKLVKEMEGYLPKMNEIVQKMGSEL"
BASE COUNT          912 a          525 c          433 g          927 t
ORIGIN      
        1 tacaacattc ccgacagttc aataatatcc cctatcctta tacaatcatc cttcatagtg
       61 acaaaatagt taaaaccgtt attgcatttc aatataactt taaacttatt cacttgatcc
      121 ttaactattt ccgttacata ccccgtcaat cgaacttctt tcctgttttc gtttgaatag
      181 tcactttcta tattcataaa cagaagacgt gaatagaaga aaaatacggt aaatgcaatt
      241 acaaggaatg aaagaagcaa aaccttaaca atcaccgata cgaagttatc cttagaataa
      301 atataaagaa cgctaacgga aatcaaccct gagataaata aaaccgtaat attaaagttt
      361 gtactcaaga ttattgccgt tattgaatat agcgttaaca aaggaataaa ggattttttt
      421 attaccattg ttatacgata ttttatttaa ctgtatcaat gaataaacaa aatcaaaaag
      481 ttataaataa aaaaatcaaa aagttaaaat aagcacagga aaccgtcata ttatttctct
      541 ttagtgagtt tacctatttc cttattaact ctttctctca gattgataat atattcgata
      601 tcttcaggga atttactgaa ggatattttt ccgttactct caataatctt catcacatgc
      661 tcataggaag tcagtgactc cagctttaag aatgctctca aatcctgtaa tgcttcatta
      721 aatactacaa gtcttaagga tttaagcggt ttttggtcat gccccggata taccgaatat
      781 gcatctcccg caggagcgaa gaaatcgccg tcggttataa ggaaggggtt gacaagtttt
      841 ctggaatatt ggttatagta gaaattatag ccccattgta aaaatccttt aattccgtat
      901 ttatataact gagtgcctat tattctgttt ctctgtgacg gcattgcaat aaatctgtta
      961 ctgacgtcaa taaactgacc gcaacagtaa tatgtccata gattatctat gttttgttta
     1021 agaaagggtt caatcttatc cgttgcagga atgggagatg ttacaattcc ctgtttgtag
     1081 aattctatgg aagataaagc atcggttaca agataaccgt ctaaaagatc gataatgctg
     1141 tttcttgcat ttctgtaccc ttctatattc ttctcattag gctcgtcgga aatatgaaag
     1201 aaacaccttt tatctacacc tttattctta agataaatca aaagattgga tataaaatcc
     1261 cttagaaaag aaatatattc atcggaagtt gcatcggtct cccatccgaa tatccttttc
     1321 ttacgaccgt tgacatatgc tacaactttc ggtgcatgct tagcacccca ttgggtaaaa
     1381 aagtgagaga tttcgaaata ttcaaatcct atctcattac acatatctat ccatttatcc
     1441 agtaaatcat acgagtattc ataacctgat gatgtttttt taatatccac cagttgcatt
     1501 gtcggtcgct cccctcctat cttagtgtcc aaaggcggtg taagtaccgg agtcagtacg
     1561 gcattaatac cgtactcata agccgttttt aggaaattct tcataattgt aaagtgtttt
     1621 ttgcttaacg gtttaactgc ataataagaa gctaaacagt caccgtgaaa ccactgtgta
     1681 aatgtaatct ttttatcgga taacatagaa ttgattatat taaccgatac atccgttgaa
     1741 gcaaccgtat taccgtcatt atcggtaaaa tagcatttaa taacataatc tccgtaaaaa
     1801 catgtattat cacttaaatc cacctctata aacagcgact gcagaagatt aggtacgaaa
     1861 tagacattgt cttttttatc atacggacat aataaatcgg gataacaatg gggtacttta
     1921 cgctgataat tattgatcgg taaattgtga tatacgggca gcctgacagc aaccgattcc
     1981 actttataaa cccgtataaa atccttcaac tccgattgta tatgaaaata acaggggctt
     2041 ttatccttta aagtatcgct tgtcatagta taagcaatct ggaaattaag tttttcattc
     2101 agtaaacagg aaaaggaagt atattgcttc ttgtcttgta tattatcgtc taaaaaacat
     2161 ttctccaatg atgatacaca ctttacaatg taattattca tagctcactt cccatttttt
     2221 gcactatttc attcattttg ggtaaatatc cttccatttc cttaaccagc ttaaattgcg
     2281 ttctggcgcg tataagatta tggttttctt tgtgtaccgc aaaatatatg tcgttattca
     2341 gataatcggt gagaaatctc atgcctatct ccatggttat aagtttaatg gaaaacggca
     2401 gtagtgataa ctcaacggca tttatactgt tctttacggc tgctaaaaaa ccttttgtga
     2461 aatatatgaa gttatcagta acgaaatgaa ctttatcaag attttcctcg tcttcctcag
     2521 cggaagaacc tgaaattctc aacgcatccc cgtagtcgta aagtattgaa ccgggcatga
     2581 cggtatccag atccaaaata cagataggtt tatcggtttc attatcaaat aaaagattgt
     2641 tcagttttgt atcattgtgc gtaactctta aaggtatttt acctgacttt atgttctcca
     2701 atattactcc gcaatcggct tttctttgat ataaaaagtt aatttcatcg gaaacagatg
     2761 agactcttcc cgcaacatcc ctctttacgg acatctc
//
LOCUS       DAOPWC010000190         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37459_length_2793_cov_2.674945, whole genome shotgun sequence.
ACCESSION   DAOPWC010000190 DAOPWC010000000
VERSION     DAOPWC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37459_length_2793_cov_2.674945"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1941)
                     /locus_tag="PKJ65_04810"
     CDS             complement(214..1941)
                     /locus_tag="PKJ65_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HNZ41190.1"
                     /translation="MNRRISYKLITVLITILCLLTICGCSNQTKEEKDIFDYIIEDSS
                     VMITKYKNMEVAENVVIPDTINGYPVKGIDNEVFMNMTDIINIIIPDTVSHIGYNAFK
                     GTKWYENLNDEFVIAGDKVLIKYNGTERNVEIPKTVKYLSACAFTDRNDIWDIKLHEG
                     ITIIPDYCFYNCKFTYLHPTEQIAFIGESAFEESNIAEFTVPDIINTVPKRAFCNCSN
                     LSTITLHDNVENIYEKAFGYSSLYALKVKGYENEEKDYQYGFYGQIVLPDNIKYIADD
                     VFEGCSNIKDLSAINIKEGIKSITKENFYYFESPGHVYIPRSVTYIEDKVFKECPDIH
                     LMVYENSYAHEYAKNNQYRYILRPLFADALFERILKSGFREKGWEFTHENMLKVESIL
                     IYPSFFIHNDIEETPYILTINGDGPFGCVENDRITRITSLEDLKYFENLTSLEIDSLT
                     KISDISPIVNCPNLQHFTVRYANIRDISPLLQLKTLNWLGLDNNPIEDFSPIGQLTDL
                     TSLTLNNTGVKDLKFLENLTNLEVLDLCNNQFDDVTPLLSLKKIRHLYLDEQKLKDYN
                     NLKKTDYAD"
     gene            2123..>2793
                     /locus_tag="PKJ65_04815"
     CDS             2123..>2793
                     /locus_tag="PKJ65_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNZ41191.1"
                     /translation="MKKTILSLNCGSSSLKYNVFRINDDLTVTPVLNGNAEEIGNPQR
                     STMTFKINGEKNKYPVPLETHEDAINEMISVFRKNGITKESICAVGHRIVHGGPVYTK
                     STLITDDVKKQIRELFSVAPLHNPQNYIGVEEIEKRLPGVPNVAVFDTAFHGTMGEEA
                     FRYAIPEKWYRNFLIRRYGFHGTSHLYVSKRAAVLLKTPYNKFTCITAHLGNGSRITK
                     VETGT"
BASE COUNT          878 a          491 c          493 g          931 t
ORIGIN      
        1 gatatattcg taggtctgtt cctctctgat tgacagataa tctttcattt atttatacct
       61 ccgctgcttt acagatagat tatagcatgg atagggagcg gatgtaaaca aatgtttgag
      121 ttgcggatat atataagatc tgatagatat gacgtgatga ataattattg caaaagtgtc
      181 aagtaaaaca ctgcaaaagt gtcaagtgag atactaatct gcataatcag ttttctttag
      241 attgttgtaa tctttgagtt tttgttcatc gagatacaga tggcgtattt tttttaaact
      301 caatagaggt gttacgtcat caaattgatt attacataaa tctaaaactt caagatttgt
      361 taaattctct aagaatttca aatcttttac tccggtatta ttcaacgtaa gcgaagtcaa
      421 atccgttaac tgtcctatcg gactaaaatc ttctataggg ttattatcta aacctaacca
      481 gttcagtgtt tttaattgta gaagagggct tatatccctt atatttgcat atcttacagt
      541 aaagtgctgt agattagggc aatttactat cggacttata tcacttattt ttgttaagga
      601 atcaatttcc agcgaagtta aattctcaaa gtatttcaag tcttccaatg aagtgattct
      661 tgttattctg tcgttctcaa cacatccaaa gggaccgtct ccgtttattg tcagaatata
      721 aggagtttct tcgatatcat tatggataaa aaaactggga tatatcaata tactttccac
      781 tttaagcata ttctcatgtg taaattccca tcctttctcg cgaaatcccg atttcaaaat
      841 tctttcaaac agagcgtctg caaacagcgg gcgtaatata tatctgtatt ggttgttctt
      901 tgcatattcg tgagcatagg agttttcgta aaccattaaa tgaatgtcag gacattcttt
      961 aaatacttta tcctcaatgt aggttacaga acggggaata tagacatgac cgggactttc
     1021 aaagtagtag aaattctctt tagttatact ttttattcct tctttaatat ttattgcgga
     1081 taaatctttt atattgctac aaccttcaaa tacatcatcg gcaatatatt tgatattatc
     1141 cggcaatact atttgaccgt aaaatccgta ttggtaatct ttttcttcat tttcatatcc
     1201 ctttacctta agcgcatata aggagctgta accgaaagcc ttctcatata tattttcaac
     1261 gttatcgtga agagtgattg ttgacaggtt gctacagttg cagaatgccc ttttggggac
     1321 agtatttatt atatccggta ccgtaaattc agctatattg gattcttcaa aagcactttc
     1381 tcctatgaaa gcaatttgct ctgtagggtg aagataagta aatttgcagt tgtagaaaca
     1441 gtaatcgggt attattgtta taccttcatg cagtttaata tcccaaatat catttctatc
     1501 cgtgaatgca caagcagaca ggtatttgac ggttttcggt atctcaacgt ttctttccgt
     1561 cccgttatac ttaatcagca ctttgtctcc ggcaattaca aattcatcat taagattttc
     1621 ataccactta gttcctttaa atgcgttgta gcctatgtgc gatacggtat cgggtatgat
     1681 gatattaata atatccgtca tgttcatgaa aacttcatta tcaatgcctt tgaccggata
     1741 tccgttaata gtatcaggaa ttactacatt ttccgcaact tccatattct tgtattttgt
     1801 aatcattacc gaagaatctt caataatgta atcaaatata tctttttctt ctttagtttg
     1861 attagaacat ccgcatatag tgagcaggca tagaattgtt attaaaacgg ttataagttt
     1921 atacgatatt cttctattca ttttcggttc accctttaca tagttgtatc cgtaagtttt
     1981 agtatttatg ttcattagac gatttatata acagaaatgt tctgtgtaga ttatagcatg
     2041 gtttatgaag ttgacgaaag ccttttccct tgtaacacaa aaagtgcaat ggtataatca
     2101 ttaggatgaa aggaaattta ctatgaaaaa aacaatactc tctttaaact gcggaagttc
     2161 ttcacttaaa tacaatgtat tcagaatcaa tgatgatttg accgttacac cggtactgaa
     2221 cggtaatgcc gaagaaatag gcaatcccca aagaagcacc atgactttta agataaacgg
     2281 tgaaaagaat aagtatccgg taccgttgga aacacatgaa gatgccatca atgagatgat
     2341 cagcgttttc aggaaaaacg gaataaccaa agagagcata tgtgcagtag gtcacagaat
     2401 agtacacgga ggaccggtat acacaaaaag caccctcata accgacgatg taaaaaaaca
     2461 gataagagaa ttgttttccg ttgctccgct tcacaatccc cagaattata tcggagtgga
     2521 agaaatagaa aagcggcttc cgggtgttcc caatgtcgca gtatttgaca cggcattcca
     2581 cggaaccatg ggagaagaag ctttcagata tgctattccg gaaaaatggt ataggaattt
     2641 cctgataaga agatacggat tccacggcac cagccattta tatgtctcaa aaagagcggc
     2701 tgttctgttg aaaacacctt ataataagtt tacctgtatt acggcacatc tcggcaacgg
     2761 atcccgtata acaaaggtgg aaaccggtac gtg
//
LOCUS       DAOPWC010000191         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37555_length_2787_cov_3.416911, whole genome shotgun sequence.
ACCESSION   DAOPWC010000191 DAOPWC010000000
VERSION     DAOPWC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37555_length_2787_cov_3.416911"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..364
                     /gene="rpmG"
                     /locus_tag="PKJ65_04820"
     CDS             215..364
                     /gene="rpmG"
                     /locus_tag="PKJ65_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNZ41192.1"
                     /translation="MRDKIILACTECKQRNYSTKKNKKNTPDRLEFSKYCKFCRKHTP
                     HKETK"
     gene            378..590
                     /gene="secE"
                     /locus_tag="PKJ65_04825"
     CDS             378..590
                     /gene="secE"
                     /locus_tag="PKJ65_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNZ41193.1"
                     /translation="MAEKEKKSFGSKFTGFFKGIAQELKKVTWPTGKQLFRSTVTVLV
                     VCAIVAIIISLFDWGLSSLIKLLVKG"
     gene            608..1171
                     /gene="nusG"
                     /locus_tag="PKJ65_04830"
     CDS             608..1171
                     /gene="nusG"
                     /locus_tag="PKJ65_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNZ41194.1"
                     /translation="MEENLNTEITEKREEQDLARWYVVHTYSGYENKVKADLEKIVEN
                     REMQEYIQEIVVPLEEVIEEKNGKRKITFKKIFPGYVIVKMIMNDQSWYVVRNTRGVT
                     GFVGPSARPVPLTAEEVIQMRLEGMETVIDFNVGDNIRVIEGPFENFVGIIREVDKDK
                     RKVKVHISIFGRETPVELDYDQIQIIL"
     gene            1204..1629
                     /gene="rplK"
                     /locus_tag="PKJ65_04835"
     CDS             1204..1629
                     /gene="rplK"
                     /locus_tag="PKJ65_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ41195.1"
                     /translation="MAKKIIGYVKLQLPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTKNDMGLIIPIVMTVYADRTFSFVTKTPPAAVLLKKACNVESGSAVPNKTKVATVSK
                     ADLRKIAEQKMADLNAGSVEAAMSMVAGTARSMGIKVEE"
     gene            1642..2340
                     /gene="rplA"
                     /locus_tag="PKJ65_04840"
     CDS             1642..2340
                     /gene="rplA"
                     /locus_tag="PKJ65_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ41196.1"
                     /translation="MKRSKNYRASLELYDKTASYDPSEAINLVKSTAKAKFDETIELH
                     IKLGVDSRHADQQVRGAVVLPHGTGKNVRVLVFAKGPNADAAEKAGADYVGAEELADK
                     IKNENWFDFDVAIASPDMMGVVGKIGRILGPKGLMPNPKAGTVTPDVAKAVTEAKAGK
                     IEYRLDKTNIIHCPIGKASFEPEKLMDNFRTLMNAVIKAKPAAAKGQYLRSITLASTM
                     GPGIKVNVQKVLDL"
     misc_feature    2392..2527
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2535..>2787
                     /locus_tag="PKJ65_04845"
     CDS             2535..>2787
                     /locus_tag="PKJ65_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ41197.1"
                     /translation="MASEKILEVKKQQQAELREKLLSMGSVILVDYRGLTVAQDTAMR
                     SDMKKTDIEYKVIKNRLLVKAVEGTRYEGLIPHLEGPTAV"
BASE COUNT          957 a          475 c          685 g          670 t
ORIGIN      
        1 tgttagcatt aattacagta aaacggaagc tactgcaaaa ctaatttccg catgcccctg
       61 tagaacggaa cttagttatg caatctaatg aataagccgt ttgtcaaaaa atgtgttgac
      121 attatatata aagacatggt aagatatccc ttgctattag aaggctcttt ttttttgtgg
      181 aaaagtcttc aataagaaaa ggcaggaaga gaaaatgaga gataagatta tacttgcatg
      241 tacagagtgt aaacaaagaa actactcgac aaagaaaaac aagaagaata ctcctgacag
      301 gctggaattc agtaagtact gcaaattctg caggaagcat acccctcata aagagacgaa
      361 atagtaaggt gacggcaatg gcggaaaaag agaaaaaaag ttttggatca aaatttacgg
      421 gattctttaa gggaatcgcc caggagctta aaaaagttac atggccgaca ggcaaacagc
      481 ttttcagaag cacggtgacg gttttggttg tatgtgccat tgttgccatt attataagct
      541 tgtttgactg gggcttatcc tcccttatca aactgcttgt taaaggataa aacgggagat
      601 tgttttaatg gaagagaatt tgaatactga aataaccgaa aaaagggaag aacaggattt
      661 agcaagatgg tatgtagtgc atacttattc cggttacgag aataaggtta aagccgacct
      721 ggaaaaaata gtggaaaaca gggaaatgca ggaatacata caggagattg ttgtaccctt
      781 ggaggaagtt attgaggaaa agaacggaaa gagaaaaata accttcaaaa aaatcttccc
      841 cggctatgtt attgtaaaaa tgattatgaa tgaccagtca tggtatgtcg taagaaatac
      901 cagaggagtt accggttttg tcggtccctc cgcgcgtccc gttccgctga ctgccgaaga
      961 agtcattcag atgagattgg aaggaatgga aacagttatt gacttcaatg taggagacaa
     1021 tatcagggtt atcgaaggac ctttcgaaaa ctttgtaggt attatcagag aagttgataa
     1081 ggacaagaga aaagttaaag ttcatatttc aatattcggc agggaaacac ctgttgaatt
     1141 ggattatgat cagatacaga ttattcttta agcttgaaaa aatacatttg ggaggtggat
     1201 tgaatggcaa aaaagattat cggatatgta aaattgcaat tgcccgcagg caaagcaacg
     1261 ccagcaccgc cggttggacc agcactagga cagcacggtg taaacattat gggattctgt
     1321 aaagaattca atgaaagaac aaaaaatgac atgggactta tcattcctat agtaatgacg
     1381 gtctatgcag acagaacctt ttctttcgtt accaagactc cgcccgcagc ggtactgctt
     1441 aagaaggcat gcaatgtgga aagcggctct gcggtcccta acaagaccaa agttgcgaca
     1501 gtttccaaag ctgacttaag gaagattgcg gaacagaaga tggcagacct caatgcggga
     1561 agcgttgaag ctgctatgag catggttgca ggtactgcac gcagtatggg catcaaggta
     1621 gaggaatagg aggaagcgta aatgaaaaga agcaagaact acagagcaag tttggaatta
     1681 tacgataaaa ctgcctcata tgatccttcg gaagccataa accttgttaa atccacagct
     1741 aaagccaagt ttgatgaaac aattgaattg catatcaaac tgggtgtgga ttcaagacat
     1801 gccgatcagc aggtcagagg tgccgttgta ttaccgcacg gaaccggtaa aaatgtcaga
     1861 gtattggtat ttgccaaagg acccaatgcc gacgctgctg agaaagccgg agccgactat
     1921 gtgggagcgg aagaacttgc ggataagatt aagaacgaga actggtttga ttttgatgtt
     1981 gctatagcca gtcccgacat gatgggtgtg gtaggaaaaa taggacgtat tttggggccc
     2041 aaaggattaa tgcctaaccc caaagcaggt accgtaacac ccgatgtggc aaaagccgta
     2101 accgaagcca aagcaggtaa gattgagtac agactggaca aaacaaacat tatccattgt
     2161 ccgatcggca aagcatcgtt cgaaccggaa aagttgatgg acaacttcag aacacttatg
     2221 aatgcggtta ttaaggcaaa accggccgca gcaaaaggac aatatctgag gagtattaca
     2281 ttggcttcga ccatgggccc cggaatcaag gtaaatgtac agaaagtact tgacttgtaa
     2341 gggcttatgg cttattattg gaaaagttgc ggattatatc cgtgatgaaa agtaaataga
     2401 gtaaatctgc cgcagacagc aggtgcgatt cggaagaaaa gcgtaaatcc cgccgaggaa
     2461 gatacaagat tgatttatta tccaatcctc gtatgcgtgc agtatgcgag gattttttat
     2521 aaggaggtaa aataatggct agtgaaaaga tattggaagt caagaaacag caacaggcgg
     2581 aattgagaga aaagctgttg tccatgggat ctgtcatact ggtggattac agaggactta
     2641 cggttgctca ggataccgca atgcgttctg acatgaaaaa aactgatatc gagtataagg
     2701 tcattaaaaa caggcttctt gtaaaagctg tggaaggtac cagatatgag ggtttgatac
     2761 ctcatcttga aggtcccact gcagttg
//
LOCUS       DAOPWC010000192         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37711_length_2779_cov_2.768722, whole genome shotgun sequence.
ACCESSION   DAOPWC010000192 DAOPWC010000000
VERSION     DAOPWC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37711_length_2779_cov_2.768722"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PKJ65_04850"
     CDS             <1..1531
                     /locus_tag="PKJ65_04850"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ41198.1"
                     /translation="NIEKIIREEMDKKGALELLMSALIPKELLEQSGRWEVFGPEMFR
                     LKDRSERELCLAPAHEESFIITARNEIRSIKNLPLTLYQIQTKFRDELRPRYGIIRSR
                     EFLMKDAYSFDKDESGLDKSYNDMHDAYCNIFARCGLDVVIADGAGSEEFMVLSDIGE
                     DKVVTCTVCDYAANQEKAEAVIHNTASSDTLLNKELLHTPNIKTIDDLCSFMGCSSVN
                     FAKTLILVADGQPVMAVIRGDHDLSLKKLAGLLKCDELSLAENHLVEKLTGAAVGFAS
                     PVGMKCKVVLDNQLKNMTNFVTGANKTDYHYINVNLKDFTSYSFGDIRAVCENDVCPK
                     CGGGIRISSAVEVGHIFKSGDKYSKALDCVYTDEFGEKKPMIMGCYGIGLDRTLAAVI
                     EQHNDEKGIVWPMEVAPYKVYILPTLVSDPVIFDMAVKMHDQLVAMGIDVILDDRNER
                     AGVKFNDADLLGIPLRVTVGRNAKDGMVELKKRNEEQAVLVTYSRALQSITENVREYT
                     A"
     gene            complement(1473..2120)
                     /locus_tag="PKJ65_04855"
     CDS             complement(1473..2120)
                     /locus_tag="PKJ65_04855"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ41199.1"
                     /translation="MERFEERMEKTADRLKKLYEEYIEKVKQSNKDYDFQFVFSDGLM
                     RKGIMLIGEAPGKDEVAQGKPFVGMAGAKLKEFMESLKLERDDIFVTNAVKYRPYKIN
                     PKSGKKSNRTPTKKEIQLSLYCLEKEIEIIKPKLILTLGNVPLRALKKDFTLNIGQWH
                     GKQTEYKGITHFPLYHPASVIYNKSLQSVFEQDLEAVKQYILLHFLLYFVMPGYM"
     gene            complement(2075..2626)
                     /locus_tag="PKJ65_04860"
     CDS             complement(2075..2626)
                     /locus_tag="PKJ65_04860"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ41200.1"
                     /translation="MSEWLQLEKQCNECKKCSLWENRKNVVIGRGNKSAAFMLIGEAP
                     GEEEDIQGRAFVGRAGQLLSMALSAMGIEEDNYYICNIIKCRPKNNRTPYEQEIQQCL
                     PYLDRQIELVQPEVIVLLGNTAAASLIDKNIRITQQRGTWHEYKGIPVMLTYHPAALL
                     RDESKKIDMWRDLKNAWKRLQTA"
BASE COUNT          749 a          586 c          619 g          825 t
ORIGIN      
        1 gaacatagag aagataatcc gggaggaaat ggataaaaag ggtgcactgg aacttcttat
       61 gtccgccctt atacccaagg aacttttgga acagtcggga agatgggaag tcttcgggcc
      121 ggaaatgttc cggttgaaag accgttcgga aagagagctc tgtttagccc ccgctcatga
      181 agaatccttt ataataaccg caaggaacga aatccggtcg ataaagaatc tgcctttaac
      241 cttgtatcag atacagacca aattccgtga cgagttgcgt ccgcggtacg gaataatacg
      301 ttcaagagag tttctgatga aggatgcata cagtttcgat aaggatgagt ccgggctgga
      361 taagtcatat aacgatatgc acgatgcata ttgtaatata tttgcccgtt gcggtctgga
      421 tgtggttatt gccgatgggg cgggctcgga ggaatttatg gtattgagtg atataggaga
      481 agataaggtg gtaacctgta cggtatgtga ctacgccgct aatcaggaaa aagcagaagc
      541 ggtaatccat aatacagctt cttccgatac attgctaaac aaagagcttt tacacactcc
      601 gaatataaag acaattgacg acctttgctc cttcatgggt tgttcttccg ttaactttgc
      661 aaaaaccttg atactggtag ccgacggcca gccggttatg gctgttataa ggggggatca
      721 cgatttatca ttgaagaagc ttgcaggttt gcttaagtgt gacgagcttt cgcttgcgga
      781 aaatcatctg gtggagaaac ttaccggtgc tgcggtggga tttgcctctc ccgtaggcat
      841 gaagtgtaaa gttgttttgg ataaccagct caagaatatg accaacttcg ttaccggagc
      901 caacaagacc gattaccatt atataaatgt aaatcttaag gatttcacaa gttattcttt
      961 cggggatata agggcggtat gcgagaacga tgtatgcccc aaatgcggtg gcggtataag
     1021 gataagcagt gccgtggaag tgggacacat attcaaatcg ggtgataaat actccaaggc
     1081 tttggattgt gtgtataccg atgaattcgg agagaaaaag cccatgatta tgggctgtta
     1141 cgggatagga ttagacagaa cgctggctgc ggttatagag cagcacaacg atgaaaaagg
     1201 catagtgtgg cccatggaag ttgcaccgta taaagtatat atattaccta cattggtaag
     1261 cgatcccgtc atattcgata tggcggtcaa gatgcatgac cagctggtcg ctatgggtat
     1321 cgatgtcata ctggatgacc gcaacgagcg ggcgggtgtt aagttcaatg atgccgattt
     1381 gttgggcata ccgttaagag taactgtggg cagaaatgcc aaagacggta tggtggaact
     1441 taaaaaacgt aatgaagagc aggctgtgct tgttacatat agccgggcat tacaaagtat
     1501 aacagaaaat gtaagagaat atactgctta acggcttcaa ggtcctgttc aaatacgctt
     1561 tgcagacttt tgttgtatat gacgcttgcg ggatgataca gaggaaaatg tgttatccct
     1621 ttatattccg tctgtttacc gtgccactgc cctatattca aagtaaaatc ttttttcagt
     1681 gcacgtaaag gaacattacc caaggtaagt ataagtttcg gtttgattat ctctatttct
     1741 ttttccagac aatacaggga aagttggatt tccttttttg tcggggtacg gttgctcttt
     1801 ttacctgact tgggattaat tttatacggt cggtatttta ccgcattggt tacaaatatg
     1861 tcatctcttt ccagcttcag ggattccatg aactctttaa gttttgctcc agccattccg
     1921 acaaaaggtt taccttgtgc aacttcatct tttcccggag cttcccctat aagcataatg
     1981 cctttacgca tcaagccgtc ggaaaagaca aactggaaat catagtcttt gttactttgc
     2041 tttacctttt ctatatattc ctcataaagc ttttttaagc ggtctgcagt cttttccatg
     2101 cgttcttcaa atctctccac atatctatct tcttggactc atcccgcaaa agtgccgcag
     2161 gatgataggt aagcataacc ggtatccctt tatactcatg ccatgtgcct ctttgctgcg
     2221 ttatccttat gtttttatct ataaggcttg ctgctgcggt attgcccaaa agcactatca
     2281 cttccggttg caccagttca atctgtcggt ccagataagg taaacactgc tgtatctcct
     2341 gttcataagg ggtgcggttg ttcttaggtc tgcattttat aatattacag atatagtagt
     2401 tatcctcttc tatacccata gcagacaaag ccatggataa caactgtccc gctcttccca
     2461 cgaaagccct gccctgtata tcctcttctt ccccgggagc ttctcctata agcataaagg
     2521 cggcagactt attgcctctg cctattacaa cattcttcct gttttcccat aaagaacact
     2581 tcttacattc attgcactgt ttttccagct gtaaccattc actcatactt cgttatcctt
     2641 tatatcccaa gtcttttgca atactctcta atttcgccaa ccgcttctgc acagtggatt
     2701 ttgacacctt taccttaagt cgctcggcaa tctcgttata ctccgtcaac tcttcgttaa
     2761 gtctcaacag tgcagaagc
//
LOCUS       DAOPWC010000193         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_37820_length_2774_cov_3.773446, whole genome shotgun sequence.
ACCESSION   DAOPWC010000193 DAOPWC010000000
VERSION     DAOPWC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37820_length_2774_cov_3.773446"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKJ65_04865"
     CDS             <1..472
                     /locus_tag="PKJ65_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNZ41201.1"
                     /translation="RFVIGGPMGDSGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDR
                     SAAYAARYAAKNIVASGVAKRCEIQIAYAIGVARPVSVVVDTFGTASVDDERIAKAVM
                     QVFDLRPYSIIQSLNLKSPIYKQTATYGHFGRSDLNLPWEKTDKADILRDMLVK"
     gene            568..1140
                     /locus_tag="PKJ65_04870"
     CDS             568..1140
                     /locus_tag="PKJ65_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ41202.1"
                     /translation="MDGIIQYIFPNEKYDEINKLKFQAYIKDNHIYFVEYDEVISSVI
                     KYAKYTRHPASFVQIGHEMGVILKDMCVSADNVIYVPMHVKDQAKRGFNQSLILARKI
                     SECTGIPICHKALKKNKKTLHQASLSASLRGRNLAGAFVADDRYTAGRSFILVDDIYT
                     TGTTLNQCRKVLLEKMAKSVLLATITKRII"
     gene            1198..2568
                     /locus_tag="PKJ65_04875"
     CDS             1198..2568
                     /locus_tag="PKJ65_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNZ41203.1"
                     /translation="MFYSERYRIIQPLLKNSKETKYLVFDEMDKCKKVISENIGKFES
                     GGDIEQLSGIEHTGLPKITDYFEVNECCCIVQEYIEGESLKNYVERRGVLDLYEALDI
                     FIKLLYIVKFLHDIKPISIIHGSIHHDNVILIEDNVYLTDYGIKEEKKNPYQAPEAIL
                     GIKKSKQQDIYSLGMLLNFMITGGFKKPGYSQKSLTGTDSLIARCTNANFNNRFEDTG
                     ILITECERLARNLTKDSSQPMSKLIAVNGCHDAVFELAQSLGKALSENVLIIDADILQ
                     PSFEPDSYILNDNLQSMMDRTDKGEDINKALKKAKISSKVYIIPSVIRIEGYENITFT
                     SFYNLLIRLAEDFKVIFIKCSDFIYDSLTVNTYIISDQIVYIMSDPLNDMLLFNEISR
                     YLCERHDIDNRRFNAFIYKKRIKNIVTAGIKENLYGEYLGYINSRSRGYAKDLTGITD
                     KIRRKL"
     gene            2570..>2774
                     /locus_tag="PKJ65_04880"
     CDS             2570..>2774
                     /locus_tag="PKJ65_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41204.1"
                     /translation="MAVLKSLSILNDLPDNSRYAKGSYPLSKVVTDVTKEILSASPEL
                     VVNAYYDNRQRSFLENRILNILDR"
BASE COUNT          954 a          455 c          597 g          768 t
ORIGIN      
        1 aaggtttgtt ataggcggac ctatgggcga ctcgggattg accggaagaa aaatcattgt
       61 agatacatat ggcggtatgg gacgtcacgg cgggggagct ttttccggta aagatccttc
      121 aaaagtagac cgttccgcag catatgcggc aagatatgcg gctaaaaata tagttgcaag
      181 cggtgtggct aaaagatgcg aaatacagat agcatatgca ataggagtgg cacgcccggt
      241 atccgttgtg gtggatacat tcggtaccgc ttcggtggat gatgaacgca ttgccaaagc
      301 ggtaatgcag gtatttgatt tgcgcccgta ctctattatt caatcgctta atcttaagag
      361 ccctatttac aaacagacgg ctacttacgg acacttcgga agatccgatc tcaatctgcc
      421 gtgggaaaag accgataagg cggatattct gagggatatg cttgttaaat aggttgttta
      481 taagattaat aacaggatgg atttaaaaac agagactact tgaagtagtc tctttaatag
      541 cacaattatt atacatataa tgtcggaatg gacggtatta ttcaatacat atttcccaat
      601 gagaagtatg atgagattaa taaacttaaa tttcaggcat atataaagga taatcatata
      661 tattttgtag aatatgatga agtgatttct tccgttataa aatacgccaa atatacccgt
      721 catcccgcgt cttttgtgca gatagggcat gaaatgggag taattctgaa agatatgtgt
      781 gtaagtgcgg acaatgtaat atatgttccc atgcatgtta aagatcaggc aaaacgaggt
      841 ttcaatcaat ccctgatact ggctcgtaag atttccgaat gtaccggtat acctatatgt
      901 cataaggcat tgaaaaagaa caaaaaaacc ctacatcagg catcgttaag tgcgtcatta
      961 aggggaagaa atttagcagg agctttcgta gcggatgaca gatatacggc cggaagaagc
     1021 ttcattcttg tggatgatat atatacaacg ggcactactc tgaaccaatg caggaaggtt
     1081 cttttggaaa aaatggctaa atcggtgctt cttgctacaa taacgaaaag aattatataa
     1141 ttgtatattg acaattattg taaatatatt atcatgtacc tgtaatcggg aggaattatg
     1201 ttttacagtg aaaggtacag gataatacag cctttgctta agaactcaaa agagacaaaa
     1261 tatcttgtgt tcgatgagat ggataaatgc aagaaagtaa taagcgagaa tataggaaaa
     1321 tttgagtcag gcggtgatat tgaacagtta tccgggatag aacacacagg attacccaag
     1381 ataaccgatt atttcgaggt taacgagtgt tgttgcatag tgcaggaata tatcgaaggc
     1441 gagtcattaa agaattatgt ggaaagaaga ggcgttctgg atttatatga agctttagat
     1501 atatttataa agctcctcta tattgttaaa ttccttcatg atataaagcc tataagtata
     1561 atacacggta gtatacatca tgataatgtc atacttatcg aagataatgt ctatttaacc
     1621 gattacggta tcaaagaaga gaagaagaat ccctatcagg caccggaggc tatattgggt
     1681 attaaaaaaa gtaagcagca ggacatatat tcattgggaa tgttgttgaa ctttatgatt
     1741 accggcgggt tcaaaaagcc tggatattcg caaaaaagtc ttaccggtac cgattcgctg
     1801 attgcaaggt gtacaaacgc caactttaat aaccgattcg aagataccgg tattctcatt
     1861 accgagtgtg aaagactggc aagaaatttg acaaaagaca gctcacaacc tatgtcaaaa
     1921 cttattgccg taaacggatg ccatgatgct gtatttgagc tggcacaatc tctcggtaaa
     1981 gctttgtcgg aaaatgtact aatcatcgat gccgatatac tgcagccttc ctttgaaccg
     2041 gacagctata tattaaatga taatctgcag agcatgatgg acagaacgga taagggagag
     2101 gatataaata aggcattaaa aaaagcaaaa atctcttcca aggtatacat aataccaagt
     2161 gttatacgta tagaaggata tgaaaacata acttttacct ctttttacaa tcttcttatc
     2221 cgactggccg aagatttcaa ggttattttt ataaagtgtt ccgacttcat ctatgactcg
     2281 ctgaccgtaa atacatatat catcagcgac cagatagtat acatcatgtc cgaccctttg
     2341 aatgacatgt tgctttttaa tgaaataagc agatatcttt gcgaaaggca tgatattgat
     2401 aacaggcgat tcaatgcatt tatctataaa aaaagaatta aaaatatagt aacggccggg
     2461 ataaaggaaa atctgtatgg tgaatatctc ggatatataa acagtcggag cagaggatat
     2521 gccaaagatt taaccggtat taccgataag ataaggagga aactgtgata tggcggtact
     2581 taaaagctta tccatattaa acgacttacc cgataacagc agatatgcca agggcagtta
     2641 tccgctgtca aaagtagtca ccgatgtgac caaagagatt ttaagcgctt caccggaact
     2701 ggttgtcaac gcatattacg ataacagaca aagaagtttt ttagaaaaca gaatattgaa
     2761 tatattggac agac
//
LOCUS       DAOPWC010000194         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_38053_length_2763_cov_3.488552, whole genome shotgun sequence.
ACCESSION   DAOPWC010000194 DAOPWC010000000
VERSION     DAOPWC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38053_length_2763_cov_3.488552"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="pyrE"
                     /locus_tag="PKJ65_04885"
     CDS             complement(<1..599)
                     /gene="pyrE"
                     /locus_tag="PKJ65_04885"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNZ41205.1"
                     /translation="MKRAIAEHLLNIKAVFLSPDKPFVWASGIKSPIYCDNRITLSDH
                     FVREDIERGLAEIIKSNYPRCEAVMGTSTAGIAHAAITAHILKLPMGYVRSAPKDHGR
                     GNRIEGKLLPGQKVVVIEDLISTAGSVIDTVETLREEGAEVLGIASIFTYGMEKGLQR
                     LKEANVTNISLTDFDTIIDVACEKGFIKSEDKERLSAFR"
     gene            complement(596..1309)
                     /gene="pyrF"
                     /locus_tag="PKJ65_04890"
     CDS             complement(596..1309)
                     /gene="pyrF"
                     /locus_tag="PKJ65_04890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ41206.1"
                     /translation="MKKDVIVACDFPSRSEVMEFLQLFGKRKPFVKIGMELFYSEGPE
                     IVREIKKRGYKIFLDLKLHDIPNTVERAMRSLSMLDIDMVNLHTAGTIKMMQAALRGL
                     VRPDGTRPILIGVTQLTSTDEKELKEQLLVNGAMDDVVIAYAKLGVAAGIDGVVCSPL
                     EAGKVKKECGEKFLTVTPGIRFKDSKKDDQARITTPYEASRMGCDYIVVGRPVTQATD
                     PVGAYERCIREFVYGKESE"
     gene            complement(1306..2220)
                     /locus_tag="PKJ65_04895"
     CDS             complement(1306..2220)
                     /locus_tag="PKJ65_04895"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ41207.1"
                     /translation="MVNLSVNLSGLTLSNPIIPASGCFGYGKEFAEYYDINILGSFCI
                     KGTTKEPRFGNETPRIAESCSGMLNSVGLQNPGIEHVVQQELKEIRAYYSKPVIANIS
                     GFSVEEYEYCVRCLRGIDNVGLIEVNISCPNVEQGGMSFGTDPEMAAKVTKAVVKVAD
                     KPVYVKLTPNVTDITEIAKACESEGASGICAINTLSAMRIDIRKQKPVLANITGGLSG
                     PAVFPVALRMVYQLYKEIKIPIIGIGGISKVEDVIEMMMAGATAVQIGSLNLVDPYIC
                     KTIINQLPGMLEQLGIDDINKIVGAAHR"
     gene            complement(2214..>2763)
                     /locus_tag="PKJ65_04900"
     CDS             complement(2214..>2763)
                     /locus_tag="PKJ65_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNZ41208.1"
                     /translation="LIFKVAGQGTKALSMMKASSQLDILCGLGNGYDVNVSMYEPLLI
                     GGGCGVPPLYYLAKTLKNKGITPCVILGFNKKEDVFYEDEFKALGCNVCVCTVDGSYG
                     YIGLVTDIMDMFDYDYTFSCGPEGMLKAIYDKSIVSGQFSFEERMGCGFGACMGCTCK
                     TKYGYKRICKDGPVLFKEEILW"
BASE COUNT          696 a          736 c          452 g          879 t
ORIGIN      
        1 ttcctgaatg ccgataacct ttccttgtct tccgacttta taaatccctt ttcacatgcg
       61 acatcaatta tcgtatcaaa atccgtcaac gatatattgg tgacattcgc ttccttaagt
      121 ctttgaagcc ctttttccat accgtatgta aatatgcttg ctatacccag gacctccgct
      181 ccctcttctc ttaaagtctc taccgtatcg ataacacttc cggcagtgga tatgaggtct
      241 tcgataacca caactttctg tccgggaagc agctttcctt caattcggtt tcctcttccg
      301 tggtcttttg gcgctgaacg cacataaccc atgggaagtt tcaatatatg agcggtaatg
      361 gctgcatgag caatacccgc cgtactggtt cccataactg cttcacatcg cgggtaattg
      421 ctttttatta tctcggcaag acctctttca atatcttccc tgacaaaatg atcggataac
      481 gtaattctgt tatcacagta tatgggactt ttaattccgc ttgcccagac aaaaggttta
      541 tccgggctta aaaacaccgc tttgatatta agtaaatgtt ccgctattgc tcttttcatt
      601 cgctttcctt tccgtaaaca aattcccgta tgcatctttc atatgctccc accggatcgg
      661 ttgcctgcgt tacgggtcta ccgactacaa tatagtcgca tcccatcctg gaagcttcat
      721 aaggtgtggt tatgcgtgcc tgatcatctt tcttgctgtc tttgaaccgg attcccggtg
      781 tcaccgttaa aaatttttca ccgcattctt tctttacttt acctgcttcc aaaggtgaac
      841 agaccactcc gtctattccg gcagcaactc caagcttggc gtaagctatt accacatcgt
      901 ccattgctcc gttgacaagc aactgctctt taagctcttt ttcatcggtg gaagtaagct
      961 gggttactcc tatcaatatc ggtcgggttc cgtccggtct tacaagacct ctcaacgccg
     1021 cctgcatcat ctttatcgta ccggctgtat gcagattgac catgtctata tccagcatcg
     1081 ataagcttct catggctctc tccacggtat tgggaatatc atgaagtttg aggtctaaaa
     1141 atattttata ccctcttttc tttatttccc tcactatttc cggcccttca gaatagaaca
     1201 gttccatccc tatctttacg aaaggttttc tctttccgaa cagttgcaaa aattccatta
     1261 cttcgcttct tgacggaaaa tcacatgcaa caattacatc ctttttcatc tgtgagctgc
     1321 tcctactatc ttatttatat catcaatacc cagttgctcc agcataccgg gcaactgatt
     1381 gattattgtc ttacatatat acgggtcgac aagattcaaa gaacctatct gcaccgcagt
     1441 cgctcccgcc atcatcatct ctataacatc ctcaaccttg cttattccgc caataccaat
     1501 tatcggtatt ttaatctctt tatataattg ataaaccata cgcagtgcca ccggaaacac
     1561 cgcaggtccc gacaaccctc ccgttatatt agcaagcacg ggcttttgtt ttctaatgtc
     1621 tatcctcatt gccgaaaggg tattgattgc acatattccc gacgctccct ccgattcgca
     1681 tgccttggct atttccgtaa tatcggtaac attgggtgtt aacttaacat acaccggttt
     1741 atcggcaacc ttcaccacgg cctttgtcac ttttgcagcc atttccggat cggttccgaa
     1801 actcatccct ccctgctcga cattgggaca acttatattc acctctataa gtccgacatt
     1861 gtctattcct cttaaacatc ttacgcagta ctcatattcc tctacggaaa accccgaaat
     1921 gttggcaatc accggtttgg agtaatacgc ccttatttct ttaagttcct gctgcacgac
     1981 atgctctatt cccggattct gcaaccccac actgttaagc attccgctgc aggattctgc
     2041 aattctgggg gtctcgtttc cgaatctcgg ctcttttgtt gtgcccttta tacaaaaaga
     2101 acctaatatg ttgatatcat aatattctgc gaattctttt ccgtatccga aacatccgct
     2161 tgccggtatg ataggattgg ataatgtaag tccgcttaaa ttaacagata agttcaccat
     2221 aatatctcct ctttgaataa aaccggtccg tccttgcata ttctcttgta cccgtatttt
     2281 gtcttacaag tacatcccat gcatgctccg aaaccgcaac ccattctttc ttcaaaagaa
     2341 aactgtcccg atacaatact tttgtcatat attgctttta acataccttc cggtccgcag
     2401 gagaaagtat agtcgtaatc aaacatatcc ataatatctg ttacaagtcc gatatatccg
     2461 taacttccgt ccacggtgca gacacataca ttacatccta aagccttaaa ctcatcctca
     2521 taaaaaacat cttccttttt attgaagcct aaaataacgc atggtgtaat gcctttgttt
     2581 ttaagagtct ttgcaagata atatagcggc ggtactccgc aacctccccc tatcaataaa
     2641 ggttcgtaca tactcacatt aacatcatac ccgttaccca aaccgcataa tatatccagc
     2701 tgcgaagaag ctttcatcat ggaaagcgcc ttggtgcctt gtcctgcaac cttgaatatg
     2761 agg
//
LOCUS       DAOPWC010000195         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_38365_length_2747_cov_2.753343, whole genome shotgun sequence.
ACCESSION   DAOPWC010000195 DAOPWC010000000
VERSION     DAOPWC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38365_length_2747_cov_2.753343"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKJ65_04905"
     CDS             <1..770
                     /locus_tag="PKJ65_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNZ41209.1"
                     /translation="ILHQSKDKQWLFIKMYNYEGWINKNDVAVTSEYIVKLYEKNRNF
                     IVITRPIAYLRYSHTKSDMGCVFPLVKEHDNYYTVLCPKQGADGTLYFSFDKVEKQYA
                     HVGYLPYTQKNILIQAEKFLNEPYGWGGMNGYRDCSALVMDIFRSMGIKLKRNTDQQE
                     TMTPSKTNVKGMSKQEKEQLLKTQKAGSLIVMDGHVMLYLGIIQDKPYIIHDITGYYS
                     EGSYINAYKVTVTDVYIRNSKQEEYINLFTTFLFISN"
     gene            complement(757..1476)
                     /locus_tag="PKJ65_04910"
     CDS             complement(757..1476)
                     /locus_tag="PKJ65_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNZ41210.1"
                     /translation="MDEIRGFLIIGVVISHTLFDMHYFFRIDMSWIKSPLFTAISDIG
                     AIIFILISGMVSKYSKDNINRGLKLMCVAATVTLVTHIVVPDFVVYAGILHLLAFAIV
                     LHELIKPLLVKINRIAGLVINILIALLTANLYGREISIFGVTLFKIPENIMNGGLSYL
                     LGLKIDNITVSSDYYPILPWITFFFMGFYLTEYIERQPLKSFLCRKRCSFLAKAGRNS
                     LLVYLIHQPVVMGILYLISLI"
     gene            complement(1633..1992)
                     /gene="yajC"
                     /locus_tag="PKJ65_04915"
     CDS             complement(1633..1992)
                     /gene="yajC"
                     /locus_tag="PKJ65_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNZ41211.1"
                     /translation="MFNLILAETAATAETGGRNWTSLILIVVVFVVMYAVILIPQRKK
                     DKKLKQMLADLKNGDVICTIGGIMGKIVNVQEDEITIETSIERTKMTMKKWAVRNVET
                     VEEESSDAEPTGEIESK"
     gene            complement(2016..>2747)
                     /gene="tgt"
                     /locus_tag="PKJ65_04920"
     CDS             complement(2016..>2747)
                     /gene="tgt"
                     /locus_tag="PKJ65_04920"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ41212.1"
                     /translation="PEKVMSIQNNLGADVIMAFDECAPYPAERSYIDDSLLRTARWLE
                     RCINSHNRSSEQALFGIIQGGMYKDLRIKSAKQITSFDLPGYAIGGLSVGEPAEVMYE
                     MLEHVTPLMPFDKPRYLMGVGTPDYLIEGSIRGIDMFDCVLPTRIARNGTVLTSKGRV
                     IVRDAKYSRDFTPLDDECDCYTCKHFTRAYLRHLFKSDEILALNLATLHNLKFLLDLM
                     AKIRQSIKEDKLLDFRDEFYEKYGY"
BASE COUNT          865 a          603 c          480 g          799 t
ORIGIN      
        1 caattttaca tcaaagcaag gataaacaat ggttgtttat aaaaatgtac aactatgaag
       61 gctggattaa taaaaacgac gttgcggtta cgtcagaata cattgttaaa ctgtatgaga
      121 aaaacagaaa cttcatagtc ataacccgac ctattgcgta ccttcgctac tcacacacaa
      181 aatcggatat gggctgcgta tttcccttgg tcaaagagca tgacaattac tacaccgtgt
      241 tatgtcccaa gcaaggtgcg gacggtaccc tgtatttttc ctttgataaa gtagagaagc
      301 agtatgccca tgtcggctat cttccctata ctcaaaaaaa tatactcatt caggcggaaa
      361 agtttcttaa tgaaccgtac ggttggggcg gcatgaacgg atacagagat tgttcggcgc
      421 ttgttatgga tatattccgt tccatgggaa taaaacttaa aagaaatacc gatcagcagg
      481 agacaatgac accaagcaaa acaaacgtaa aaggtatgag taaacaggaa aaagagcaac
      541 tgctgaaaac tcaaaaagcg ggatcattga ttgtcatgga cggacacgtt atgctttatc
      601 tcggtataat acaggacaaa ccctatataa tccacgatat taccggctat tattccgaag
      661 gctcctatat caatgcgtat aaagttacgg ttaccgatgt atacataaga aactccaaac
      721 aggaagaata tatcaatctt ttcaccactt ttctctttat atcaaactga taagatacag
      781 tattcccata acaaccggtt gatgaattaa ataaaccagt aaagaattcc tccccgcctt
      841 ggccagaaaa ctgcatcttt tcctgcacaa aaaagactta agaggttgcc tttcaatgta
      901 ctctgtcaga taaaacccca taaagaaaaa agtaatccag ggaaggatag gatagtagtc
      961 ggaagaaacg gttatattat ctatcttaag tcccagaagg taggataatc ccccgttcat
     1021 gatattttcc ggtattttaa ataaggtaac cccgaaaata cttatttctc tgccatacaa
     1081 atttgcagtc agcagagcaa tcaatatatt gataaccaga cccgctatcc tgtttatctt
     1141 cacaagaagc ggtttaatca attcatgaag cacaatggca aatgcaagaa ggtgcagtat
     1201 gcccgcatat acgacaaagt ccggaacaac aatatgcgtt acaagagtca ccgttgcggc
     1261 aacacacatc agtttcaatc ctctgtttat gttgtcttta gagtatttgg aaaccatacc
     1321 ggatatgaga atgaagataa tggctcctat atcggaaatg gcggtaaaaa gaggggactt
     1381 tatccaagac atatcaatac ggaaaaaata gtgcatatcg aacagagtgt gtgatatcac
     1441 aacacctatg ataagaaaac cgcgtatttc atccaaaagg tgtaacctct ctttactatt
     1501 catgtgtgca ttatattata tttaacaaca cacgacaccg atatttttta atataactgt
     1561 atttagagaa caaaaaaaga ctgttaactt tttgtttctt aacagtctct ttgtatttct
     1621 tatcaaaaaa acttacttgc tttcaatctc gcccgtaggc tccgcatccg atgactcttc
     1681 ttccacggtc tcgacatttc ttaccgccca tttcttcatg gtcatcttgg ttctctcaat
     1741 ggatgtctca atggtaatct cgtcttcctg tacattaaca atctttccca taatgccgcc
     1801 tattgtgcat attacatcac cgtttttaag atccgcaagc atctgtttca gcttcttatc
     1861 tttcttcctt tgaggaataa gaattaccgc atacattacc acgaatacaa ctactataag
     1921 tattaatgat gtccagttcc tgccgcctgt ttcagctgta gccgccgttt cagccagaat
     1981 taaattaaac atttatataa cctccgattc aatttctaat atccatactt ttcatagaat
     2041 tcatctctga aatccaaaag tttgtcttcc tttatagatt gccttatttt cgccattaag
     2101 tcaagtaaaa atttcaaatt atgtaacgtt gcaaggttta atgccaatat ttcgtcgctt
     2161 ttgaaaagat gacgcagata ggctctggta aagtgcttac atgtgtaaca atcacactcg
     2221 tcatccaacg gagtaaaatc cctggaatac ttggcatccc gcacaatgac tctgccctta
     2281 cttgtcagaa cggtaccgtt tctggcaatt ctggtgggca gcacacagtc aaacatatct
     2341 attcccctta tagagccttc tataaggtag tcgggagtac cgacacccat cagatatctg
     2401 ggcttgtcaa acggcatcag cggggttaca tgctccagca tttcatacat aacttccgca
     2461 ggttccccta cacttaaacc ccctattgca tatcccggaa gatcaaagga tgttatctgt
     2521 ttagcacttt taatccgcaa atctttatac atcccgccct gaataattcc gaacaaggcc
     2581 tgctcgctac ttctgttatg actgttaatg cagcgttcca gccatcttgc agtacgtaag
     2641 agtgaatcgt caatatagct tctttctgca ggatacggtg cacactcgtc aaacgccatt
     2701 ataacgtcgg cacctaagtt gttttgtatg gacataacct tttccgg
//
LOCUS       DAOPWC010000196         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_38631_length_2734_cov_3.455021, whole genome shotgun sequence.
ACCESSION   DAOPWC010000196 DAOPWC010000000
VERSION     DAOPWC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38631_length_2734_cov_3.455021"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2309)
                     /locus_tag="PKJ65_04925"
     CDS             complement(<1..2309)
                     /locus_tag="PKJ65_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ41213.1"
                     /translation="MSKTVIIGGVAGGASCAARLRRLDENAKIVILERGEYISYANCG
                     LPYHIGDVIKSRKALFLQTPENMRQRFRLDVRVKNEVIAIDRKKKSVSVKEIDTGRIY
                     EESYDTLVISTGSSPLRPPIPGIDSPRIKTLWTVPDMDDIKAMIQQQNVKTAAVIGGG
                     FIGLEMAENLQHAGLEVSVMEMQDQVMAPLDYEMAQLLHENITGNGVNLYLGDGVTSF
                     ADKDDKVIITLSSGKTASADLVILSIGVRPNSKLAKEAGLTLNARGGIVVDEFLRTSD
                     SDIYAVGDVIEVEDYISKERTMVPLAGPANKQGRIAADNIAGGSKTYKGTQGTSVAKI
                     FDLTAASTGANEKTLIKQGLKKGKDYETVIIMQNSHASYYPGAVPMTLKLLFSADGKK
                     LFGAQIVGADGVDKRIDVIATAIRLGGGVSQLKELELAYAPPYSSAKDPVNMAGFVAE
                     NILQGIVRFSEWNAVENNPDAVLLDVREEAEIMAFALPGAKHIPLGQLRDRINELDKN
                     KRTIVFCAIGVRSYNATRILLQNGFTDVLLYPAGTRFYRSTHYDKYDNAKRNALPVSD
                     SGDIKKYISSTASISIDCTGLQCPGPIMKVFETLQDMKDGEILEVTASDPGFMKDIGA
                     WCRRTGNTLLTNEKQGNSYVATVKKGSNSDKTAVTKETDGKTIIVFSGDLDKVLASFI
                     IANGAAAMGRKVTMFFTFWGLTALRKPKKQQVKKTFMEAMFGWMLPRGSKKLSLSKMN
                     MGGMGTAMMKKIMKDKNVDSLETLIEKAM"
     gene            complement(2302..2544)
                     /locus_tag="PKJ65_04930"
     CDS             complement(2302..2544)
                     /locus_tag="PKJ65_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNZ41214.1"
                     /translation="MKYKFRDILKRYFPIAIAAIIGAGAGYLYYLFVGCASGTCVITS
                     NPVISTLYFALLGGLLGRIISDRSCKRKNRKEEEHE"
BASE COUNT          655 a          656 c          564 g          859 t
ORIGIN      
        1 ttcattgcct tttcaatgag ggtttccagc gaatccacgt ttttgtcttt cattatcttt
       61 ttcatcatgg ctgttcccat accgcccata ttcattttgg aaagggaaag ttttttgctt
      121 ccgcgaggga gcatccaacc gaacatggct tccatgaatg ttttctttac ctgctgcttt
      181 ttaggtttac gaagggctgt cagcccccag aatgtgaaga acattgtaac ttttctaccc
      241 attgctgccg caccgtttgc gataatgaag cttgccagca ctttgtccaa atcgcccgag
      301 aaaacaatta tggttttacc gtcggtttct ttggtcactg cggttttgtc gctgtttgaa
      361 cctttcttaa ctgttgccac ataagagttt ccttgttttt cgtttgtgag tagggtattg
      421 ccggtacgtc tgcaccaggc accgatatct ttcataaatc cgggatcgga agcggttacc
      481 tccagaattt cgccgtcttt catatcttgc agtgtttcaa agactttcat aatgggaccg
      541 ggacactgca aacctgtaca atctatactt attgatgccg ttgaagaaat atattttttg
      601 atatccccgc tgtcggaaac aggaagagca tttcttttcg cgttatcata tttatcgtaa
      661 tgagtggacc tgtaaaaacg cgttcctgca gggtaaagca gaacatcggt aaaaccgttc
      721 tggagcagta ttctggttgc attatatgaa cgaacaccga tggcacagaa gactatggta
      781 cgtttattct tatccagttc gtttattctg tcgcgaagct gacctaacgg aatatgtttt
      841 gctccgggaa gagcaaatgc cattatttcc gcttcttcgc ggacatccaa cagtaccgca
      901 tcgggattgt tctctacagc attccactct gaaaaacgta ctataccctg taatatattt
      961 tccgcaacaa agcccgccat atttaccgga tcttttgccg aagaataggg aggtgcgtaa
     1021 gcaagctcca gttccttcaa ttgcgaaaca ccgccgccca gccggatagc cgtggcgatt
     1081 acatcaatac gtttatccac tccgtctgca cctacaatct gtgcaccgaa cagtttttta
     1141 ccgtctgccg agaacagcag tttaagtgtc ataggtaccg caccggggta gtaacttgcg
     1201 tgggaattct gcattataat taccgtttcg tagtctttgc cttttttaag tccctgcttt
     1261 ataagggttt tctcattggc accggttgaa gctgcggtta aatcaaaaat ctttgctacg
     1321 gaagtgcctt gtgtcccttt gtaagtctta cttccgccgg caatattgtc cgctgctata
     1381 cgtccctgtt tatttgccgg tccggcaagc ggtaccatgg tgcgttcttt ggaaatatag
     1441 tcttccacct ctataacatc gcctacagca tagatatcag agtcggatgt acgtaaaaac
     1501 tcatctacca caataccgcc tcttgcattt aatgtaaggc cggcttcttt ggccaatttg
     1561 ctgttgggtc gcacacctat ggagagtatt accagatcgg cgcttgccgt ttttccgctg
     1621 cttaatgtaa ttattacttt atcgtcttta tctgcaaaag atgtgacacc gtctccgaga
     1681 tacagattta caccgttacc cgttatattc tcatgtaaca actgtgccat ctcatagtct
     1741 aaaggtgcca tgacctggtc ctgcatttcc attaccgaca cttccagacc ggcatgttgc
     1801 aaattctccg ccatttccag gcctatgaag ccgccgccta ttaccgccgc tgtttttacg
     1861 ttttgttgct gtatcattgc ctttatatcg tccatgtccg gtaccgtcca cagcgttttt
     1921 atacgtggag agtcaatgcc cggtatgggc ggacgtaagg gcgaggagcc tgtggatatt
     1981 accagtgtgt catagctttc ttcatatatg cgtcccgtat caatctcttt gaccgacaca
     2041 ctcttttttt tgcggtctat agcaattact tcgttcttaa cccgtacatc caggcgaaat
     2101 ctctgtcgca tgttttccgg ggtctgcagg aaaagcgcct ttcttgactt tatgacatcg
     2161 cctatatgat agggcagtcc gcagtttgcg taagatatat attcgccgcg ctccagaata
     2221 acaatttttg cattttcatc caatcttctt aaacgggccg cgcaacttgc acctcccgca
     2281 actccgccga taataacagt tttactcatg ttcctcctcc ttacggtttt ttctcttaca
     2341 ggaacggtcg gatattatgc gtcccagcaa ccctcctaac agagcgaagt acaatgtaga
     2401 gataacggga ttggatgtta taacgcatgt tccgcttgca cacccgacaa agagataata
     2461 cagatatccc gctccggcgc ctataattgc agctattgcg atgggaaagt atctctttaa
     2521 tatgtctctg aatttgtact tcatgtagtt aaacctcttt ctttcttaag tatagggaaa
     2581 agtgagaaaa aactctgtga gtatgtcaca gaagaaagtt ttttttaaca tattttctaa
     2641 agagttttta tgaaaaacac ctgtaacttt cgtgttacag gtgtcttgcg atggtagcga
     2701 aggtcagatt cgaaccaacg acctaccggg tatg
//
LOCUS       DAOPWC010000197         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_38924_length_2719_cov_3.304805, whole genome shotgun sequence.
ACCESSION   DAOPWC010000197 DAOPWC010000000
VERSION     DAOPWC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38924_length_2719_cov_3.304805"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKJ65_04935"
     CDS             <1..969
                     /locus_tag="PKJ65_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41215.1"
                     /translation="TVREEHLPVPVIASANFNDIAKAVRVFVGIKKVKQSRILVVSNN
                     IDKETQSAAKKIWGCTFINCNSEELMKRYHNINDTDAKVIKDKWISQSEDILEATNQD
                     VSESAKLYLAIMEMYKEKKADAVTIDCLSLSYNDIYGNNLHMYPCLAFFQMCEDGYVG
                     VCEADIDSTITSIFTKAITGRYGFVSDPVIDTSSNQIIYAHCVSCIKLFGEQDKRLCK
                     YYIRSHAEDKKGAAVQVIFPANEQLTTVNINNVDKTACIHSAVSVGNYGGDAGCRSKL
                     AALCKSEEILNNWMPQWHRVTIFGNYTKEFVYLFKMMGFNIITEDK"
     gene            1224..1658
                     /locus_tag="PKJ65_04940"
                     /pseudo
     CDS             1224..1658
                     /locus_tag="PKJ65_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1548..2297)
                     /locus_tag="PKJ65_04945"
     CDS             complement(1548..2297)
                     /locus_tag="PKJ65_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNZ41216.1"
                     /translation="MENDKNFDKNGVYSKRVRAGKRRTYFFDVRTTRANDYYLTITES
                     KKRADDDTYERHKIFIYKEDFMKFVNAMQETVDYIKTELLPDYDFDAHNHDDYEEGQY
                     TRQDNYTVAEEKVTEDEVSQEEKEVIEEYTVYKMYNSVTNLSLKTRLALIRYYDTGTD
                     SVMFVKYSFFIEDKDRAAARLESKVTEEYVTPFDIDYDNFKKLSLFQYICTYCIGTFS
                     DIVCFFTFFSSLIGVHTYRTLVWSVITARNP"
     gene            complement(2402..>2719)
                     /locus_tag="PKJ65_04950"
     CDS             complement(2402..>2719)
                     /locus_tag="PKJ65_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HNZ41217.1"
                     /translation="GGQKQRISIARSLIKNPEVALLDDCLSAVDAKTEKTILDNLYAT
                     LQDKTVIFITHRIFAIMNFDKILVLDDGKIIEQGNHAELMDKKGIYYDIYQLQQTAEP
                     NEV"
BASE COUNT          847 a          511 c          536 g          825 t
ORIGIN      
        1 acggtacgtg aagaacacct tcccgttccg gttattgcat ctgcaaattt caatgatata
       61 gcaaaagctg taagagtatt tgtcggaata aagaaggtta aacaatcaag aatactggtg
      121 gtaagtaaca atattgataa agagacacaa tcggctgcca aaaagatatg gggctgcact
      181 tttatcaact gcaattccga agagttgatg aagcgttatc ataatattaa tgacactgat
      241 gcaaaagtca taaaggataa atggatttca caatccgaag atatactgga agcgacaaat
      301 caggatgttt cggaaagtgc aaaactctat cttgcaataa tggaaatgta taaagaaaag
      361 aaagccgatg cagtaacaat agactgctta agtttgtcat ataacgatat atacggaaac
      421 aacttacaca tgtatccctg cctggcattt ttccagatgt gcgaagacgg ttacgtggga
      481 gtatgtgaag cggatattga ttccacaata acttcgatat ttacaaaagc cataaccgga
      541 agatacggct ttgtatccga tcccgttata gacacatcat caaatcagat tatttatgct
      601 cattgcgtct cctgcataaa gcttttcgga gagcaggaca aaaggttatg taagtactat
      661 ataaggagtc atgctgaaga taaaaaagga gctgcggtac aggtcatatt tcccgccaac
      721 gaacagttga caacggtgaa tataaataac gttgataaga ctgcctgtat acattcggcc
      781 gtatcggtag gcaactacgg aggagatgcc ggttgcagaa gcaagttagc cgcactttgc
      841 aaatccgaag aaatacttaa caactggatg ccgcaatggc acagagttac catattcgga
      901 aactacacta aagaatttgt ttacctcttt aagatgatgg gttttaatat tattactgaa
      961 gacaaatagc acacagaact gctcatatcc gaaaggataa ctccctcctc aaaaagagct
     1021 tatataagct ctttttgtca gcatttcatt ctgaacaaaa aatacaataa tatgtactat
     1081 atactaaaaa acaggttttt aatgatataa aaagtattgc catttaactg tccatttgat
     1141 atgctatggg taagtttatg ggtaagttta tgggtaagtt tatgggtaag taagtgtaac
     1201 attttaatta aatggaggag aaaatggcta ttccgatcaa tattgaagaa ttacttcgtc
     1261 agcgtgtgat agagagttca cgaatagaat acaagaaaga ctggaatccg gagcctattc
     1321 tgcattccgt taccgccttt gccaatgatt ttgataatct cggcggagga tatattttaa
     1381 taggtgtgga agaacagaac ggttacccga gatttcctct caaggggctt aatgaagatg
     1441 tgttggattc tgttcagaag gaactcctta ataaatgtaa ccttattgaa ccgcgctaca
     1501 taccggtcat agagcccgca ttgatagacg gacaacatat tctcgtttta tgggttccgg
     1561 gcggtgatga cagaccatac aagtgtcctg taagtgtgta caccgataag ggagctaaaa
     1621 aaagtgaaaa agcatactat atccgaaaag gttccaatac aatacgtgca aatgtactgg
     1681 aagagagaga gctttttgaa attgtcgtaa tcaatatcaa aaggggtaac gtattcttct
     1741 gtcactttcg actcaagtct ggcagcagcc ctgtccttgt cctcaatgaa gaaactgtat
     1801 tttacaaaca ttaccgagtc ggtcccggtg tcgtaatacc tgattaaggc aagcctggtt
     1861 ttaaggctga gattggtgac ggaattatac atcttataaa ccgtgtattc ctctataacc
     1921 tctttttctt cctggcttac ttcgtcttcc gttactttct cttctgccac ggtgtaattg
     1981 tcttgtcttg tgtactgacc ttcttcgtag tcgtcgtggt tgtgtgcatc gaaatcgtaa
     2041 tccggcagca attccgtttt gatataatct acggtttcct gcattgcatt tacaaacttc
     2101 atgaaatctt ctttgtaaat aaagattttg tgacgctcat acgtatcatc gtcggcacgt
     2161 tttttacttt cagtaatcgt taagtaataa tcgtttgctc tggtagtacg tacatcaaaa
     2221 aaatacgttc ttctttttcc tgctcttact ctcttagagt acacgccgtt tttgtcaaaa
     2281 tttttgtcgt tctccacttt cttgcaattt tattgttcac aatgatattc caaaaaatac
     2341 atctataaaa attaagtagt caacaccaaa ataccaatgt taataaccac ttcgcttttt
     2401 gttaaacctc attcggctct gcggtttgct gcaattgata aatgtcataa taaatgcctt
     2461 ttttatccat cagctccgca tgatttccct gctcaataat ctttccatca tctaacacca
     2521 gaattttatc aaagttcata atggcaaaaa tacggtgtgt tataaagatg acggttttgt
     2581 cctgcagcgt tgcatacaaa ttgtcgagaa tcgttttttc tgttttggca tctaccgcag
     2641 aaagacagtc gtcgagcagc gccacttcgg gatttttaat cagagagcgg gcaatggaaa
     2701 tacgttgttt ttgtccgcc
//
LOCUS       DAOPWC010000198         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_38973_length_2717_cov_2.786627, whole genome shotgun sequence.
ACCESSION   DAOPWC010000198 DAOPWC010000000
VERSION     DAOPWC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38973_length_2717_cov_2.786627"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..868
                     /locus_tag="PKJ65_04955"
     CDS             38..868
                     /locus_tag="PKJ65_04955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ41218.1"
                     /translation="MKAGLLYSDKITTVSNTYKDEIRDPFFGENLDGVINTRSADLYG
                     IVNGLDINEYNPKTDKNIYVNYGLSDIDKKKENKKNLQRELNLDINENAPVIAMISRL
                     VPQKGLDLVRRVFDDIMSFNIQFVILGTGEEQYESFFENAHARYCGKVAALLKFDEQM
                     ARKIYAGADMFLMPSLYEPCGISQLIAMRYGTIPVVRAVGGLCDTVTQYDAQKDTGNG
                     YLFRNYNAHEMSHTVYLACQAYKERTKWQKLMKRAMKNDCSWEKAAAQYIELYEIAMR
                     "
     gene            1017..>2717
                     /gene="glgP"
                     /locus_tag="PKJ65_04960"
     CDS             1017..>2717
                     /gene="glgP"
                     /locus_tag="PKJ65_04960"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HNZ41219.1"
                     /translation="MKNGNNKNNTVIKQINEVLNRTFGKTIENATQNQLYKAFAITVK
                     NEIMDKWAQSAYTPDNKQVYYLSIEFLMGRALSNNMLNIGKRKDYQDACKQLGLDFES
                     IASREPDAGLGNGGLGRLAACFMDSLATLDLPAYGCGIRYEYGLFRQKIIDGQQVEYP
                     DAWLEDGNVWEVERPEERVEVHFGGHIKESWEGDKLRFVHSGYSTVHAVPYDMPIVGY
                     MNNRVNSLRLWKAVSPKRIDMASFNRGEYLKAVEERQMAEVLSHILYPEDKHYEGKSL
                     RLKQHYFFVSATMQMIVARFMKLNLPLSALPDKAVVHINDTHPALAIPELMRILMDEH
                     GLGWDEAWDITGRMFAYTNHTIMAEALEKWPEQLFKQILPRIYAIVKEINERFCGMVY
                     RDFPEKRNDIHKMAIIGYGQIAMAPLCIAGSYSVNGVSALHSRIIKEETFKDFADVFC
                     GKFTNVTNGVTHRRWLMLSNPMLTDLITKSIGKDWISDMVKIKALEAKADNPKFLEEF
                     DEIKRLNKRKLADYIKQTTGIVVSENSIFDVQVKRLHEYKRQLMNILRIMYDYVQIIN
                     E"
BASE COUNT          876 a          482 c          657 g          702 t
ORIGIN      
        1 gaactcgaac aggtgaaccg ggacatgtcg ttgtttcatg aaggcggggc tgttgtattc
       61 cgacaagata actacggtta gcaataccta caaagatgag atccgggacc cgtttttcgg
      121 agaaaatctt gacggtgtca ttaataccag aagtgcagat ttgtacggga tagtaaacgg
      181 tctggacata aatgaatata accccaaaac cgacaagaac atttatgtca actacggtct
      241 cagtgatatt gacaaaaaga aagagaacaa aaagaatctg caaagagaac tcaatctgga
      301 tataaatgag aatgcgcctg taattgccat gatctccaga ctggttccgc agaaggggct
      361 ggatctggtc agaagagtgt ttgacgatat aatgtctttt aacattcagt ttgttatact
      421 cggaaccgga gaagagcaat atgaaagctt ttttgaaaat gcccatgcca gatactgcgg
      481 taaggtagct gctcttttaa aattcgatga acagatggct agaaagattt atgcgggagc
      541 ggatatgttc ctgatgccct ctctttatga accgtgcggt ataagccagc ttatcgcaat
      601 gagatacggt acaatacccg ttgtgcgtgc agtaggggga ttgtgtgata cggtaactca
      661 gtacgatgca caaaaagaca cggggaacgg ctatcttttc agaaactata atgctcatga
      721 gatgtctcat actgtgtatt tggcatgtca ggcatataaa gagcgtacca aatggcaaaa
      781 gctgatgaaa agggcaatga agaacgattg ctcatgggaa aaagccgccg ctcaatacat
      841 tgagttatat gaaattgcta tgcgttaata tgatatacag gattttaagg accgacactt
      901 tgtctggtgt aacagccgga aagaaaaaca caatattttg ataataataa acttttatga
      961 tgttgtacaa gtgatgtgtt ttgtgtataa tatcatctcg gtaaaggagt acttttttga
     1021 aaaacggaaa taataaaaat aatacggtaa taaaacagat aaatgaagtt ttaaacagga
     1081 ctttcggaaa aacaatagag aatgccacac agaatcagct ttataaagca ttcgcaataa
     1141 cggttaaaaa tgaaataatg gataaatggg cacaatcggc ttatacaccg gacaacaagc
     1201 aggtgtatta tctttccatt gagtttttaa tgggaagagc attatccaat aatatgttga
     1261 atatcgggaa gcggaaagat tatcaggatg cctgcaaaca gctggggctg gactttgaga
     1321 gtattgcatc cagagaaccg gatgcaggtt tgggaaacgg aggactgggg cgtcttgcag
     1381 catgttttat ggattcgttg gcaactctgg atcttccggc ttacggttgc ggaataagat
     1441 atgaatacgg tcttttccgt cagaagataa ttgacggaca acaggtggaa tatcccgatg
     1501 catggctgga agacggaaat gtctgggaag tggaacgacc ggaagagaga gtggaagtgc
     1561 atttcggagg tcatataaaa gaatcgtggg agggcgataa actccgtttt gtccatagcg
     1621 gatattctac agtccatgcg gtaccgtatg atatgcccat agtaggctat atgaataaca
     1681 gagttaattc tttaagactg tggaaagcgg tttctcccaa gcgaatcgat atggccagtt
     1741 tcaacagagg tgaatacctg aaagctgttg aagaaagaca gatggcggaa gtgctttctc
     1801 acatattgta tcccgaagac aagcattacg agggcaaatc gctgcgcttg aaacagcatt
     1861 acttctttgt ttccgctacc atgcagatga tagttgcccg tttcatgaag ttaaatcttc
     1921 cgttaagcgc actccccgac aaagcggtag tacatataaa tgatacccat cctgctctgg
     1981 ccattcccga actgatgaga atacttatgg atgaacacgg tttgggttgg gatgaggcgt
     2041 gggatataac cggccgtatg ttcgcatata ccaaccacac cattatggcg gaagcgttgg
     2101 aaaaatggcc tgagcagctg ttcaagcaga tattacccag gatttatgcc atagtaaaag
     2161 aaataaatga acgtttctgc ggtatggttt acagagactt tcccgagaaa aggaatgaca
     2221 tacacaagat ggctattata ggctacggac agattgccat ggcgcctttg tgcattgcag
     2281 gctcctattc ggtaaacggt gtctcggcgt tacattccag gattataaag gaagagacct
     2341 ttaaagattt tgccgatgta ttttgcggaa agtttaccaa tgtaacaaac ggagttactc
     2401 atcgcagatg gcttatgttg tccaatccga tgttgaccga cctcataacc aaaagtatcg
     2461 gtaaagactg gataagcgat atggtaaaga tcaaagcttt agaagccaaa gccgacaatc
     2521 ccaagttttt ggaggagttc gacgagatta agaggcttaa caaacgcaag cttgcggact
     2581 atataaaaca gactacggga attgtcgttt ccgagaactc aatctttgat gttcaggtaa
     2641 agagacttca tgagtacaaa agacagctga tgaatatctt aaggataatg tacgactatg
     2701 tacagattat aaacgag
//
LOCUS       DAOPWC010000199         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39042_length_2713_cov_3.810384, whole genome shotgun sequence.
ACCESSION   DAOPWC010000199 DAOPWC010000000
VERSION     DAOPWC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39042_length_2713_cov_3.810384"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKJ65_04965"
     CDS             complement(<1..1539)
                     /locus_tag="PKJ65_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ41220.1"
                     /translation="MRIHINDDWQFYPSVESSESFTVRLPHSNVDMPYNCFDEAIYQK
                     ISKYEKIIHADNSWENKAILLTFSAVAHEAKVYINDRHVYTNSCGYTSFTVDISQYIE
                     WGKENRITVIADAYEKLNQPPFGNVIDYMTYGGIYREAYMDIKEKTHISDVFVSGKAK
                     AADGLCEFFAEVQIVNCTEDCEIQLSIFAVKDNECVFEKTYPMKQDIIHLSEKIPAEN
                     WDIDNPNLYRITVKLYRNKKLSDEYCDRFGFRDAIFKNDGFYLNGQKVKVTGLNRHQS
                     YAYTGYAMGKRVQINDADILKNMLNVNAVRTSHYPQSKHFINRCDEIGLLVFTEIPGW
                     QHIGDEQWKKKAVENTENMIKQYRNHPSIILWGVRINESPDDDDFYRQTNKVAKQYDP
                     YRQTGGVRCIKKSSFLEDVYTYNDFTHNGTKQVMAKKKDVTKGDVPYLITEYNGHMYP
                     TKMFDDQPHRTEHALRHARVLNAVMGDDGVAGSFGWCMNDYNTHKDFGSGDRICYHGV
                     LDMFR"
     gene            complement(1555..>2713)
                     /locus_tag="PKJ65_04970"
     CDS             complement(1555..>2713)
                     /locus_tag="PKJ65_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41221.1"
                     /translation="YGIMQALYWMGFITVYNYAAYFLSDKGFNSSQTGIILAVASILA
                     ALLQPPIAAYADKAEESKLKRIILALTSAIVVLTVLIVVIRSYPYLTAILLLLIITFV
                     HTLHSFINSFAMEYANKGIQLNFTIARAAGSLGAAFASFGVGYITAKFGADSVPIIYF
                     AMFLLLIPVTLSFKPAHDVGISSEVIKYAHAPATGVFKFFAKYKKFTGLLIGSTLIFT
                     SHNMNINFLINIVKRLGHGSAELGNVLGIAAIFEVIGMILYLIISKRLKCSMLVQISA
                     VFFTIEKLGILLSKNINHIYASYSLQMFSYAIFVPATVYYINNLMRDNDRIKGQAYLT
                     TTMTLGGVFGSLLGGTLIDALSIPSMLLIATIISAAGSLLVVVFTEKEKAI"
BASE COUNT          763 a          591 c          480 g          879 t
ORIGIN      
        1 tctgaacatg tccagcactc cgtgataaca tatcctgtca ccgcttccga aatccttatg
       61 tgtattatag tcgttcatgc accagccgaa acttcctgcc acaccgtcat ctcccatgac
      121 ggcattcagc actcttgcgt gacgtagggc atgttctgtt ctgtgcggtt ggtcatcaaa
      181 cattttggta ggatacatat gtccgttata ctcggttatg agatacggca catcaccttt
      241 tgtcacatct tttttctttg ccattacctg ctttgtaccg ttatgggtaa aatcattgta
      301 ggtatagaca tcctccagaa agctgctttt tttaatacat ctcactccgc cggtctgcct
      361 gtacggatca tattgcttag caaccttatt ggtttgcctg taaaagtcgt catcatcagg
      421 tgattcgttt attctaaccc cccacaatat tatgctcgga tgatttctgt actgttttat
      481 catgttttcg gtattttcca cggccttttt cttccactgc tcatccccta tatgctgcca
      541 tcccggtatc tcggtaaaca ccaacaaccc tatttcatca catctgttta tgaaatgttt
      601 actctgggga taatgtgatg ttctgaccgc atttacattc agcatatttt tcagtatatc
      661 ggcatcattt atctgtactc ttttgcccat tgcatatcct gtgtatgcat aagactgatg
      721 tctgttaagc cctgttactt tgaccttttg accgttaaga taaaaaccgt cattcttaaa
      781 aatcgcatca cggaatccga accggtcaca atactcatct gaaagctttt tgttacggta
      841 aagttttact gtaattctat aaaggttagg attgtcgata tcccagtttt ccgccggaat
      901 cttttcagac aaatgaataa tatcctgctt catcggatag gtcttttcaa aaacacattc
      961 attatcttta accgcaaaga ttgaaagttg tatctcacaa tcctccgtac agttaactat
     1021 ttgtacttcc gcaaaaaact cgcataatcc gtcagccgct ttggccttac cggatacaaa
     1081 cacatcggaa atgtgtgtct tctctttaat atccatgtat gcttccctgt aaatacctcc
     1141 gtaagtcata taatctataa cattaccgaa gggaggctgg tttaattttt cataagcatc
     1201 ggcaataaca gttattctgt tttctttccc ccattcaata tattgagata tgtcaacggt
     1261 aaatgaagta tatccgcaac tgttagtata tacatgcctg tcatttatat acaccttggc
     1321 ttcatgagct actgccgaga aggtaagtaa tatagctttg ttttcccatg agttgtccgc
     1381 atgaataatc ttttcatatt tcgatatttt ctgatagatt gcttcgtcaa aacaattata
     1441 tggcatatcg acattgctgt gcggcagtcg cacggtaaaa gattcggaag attcaaccga
     1501 cggataaaat tgccagtcat catttatatg tattctcata gtagcctcct tctgtcagat
     1561 tgctttttct ttttctgtaa agacaacaac cagcagggag cctgctgctg atattatggt
     1621 tgctatcaac agcattgaag gtattgataa tgcatctatc aacgttcctc ccagcagact
     1681 tccgaaaaca ccgcctaatg tcatggttgt agtcagatat gcctgacctt ttattctgtc
     1741 attatcccgc atcaggttat ttatataata gactgttgcc ggaacaaata tggcatagga
     1801 aaacatctgt aatgaatagc tcgcatatat gtgattaata ttctttgata aaagaattcc
     1861 cagtttttct attgtaaaga atactgcgga gatttgtacc agcatactgc attttaatct
     1921 ctttgatatt atgagataca aaatcatacc tataacttca aatattgcag ctatacccaa
     1981 cacatttccc agttcggcag atccgtgccc caatctcttc acaatattaa taaggaaatt
     2041 tatattcatg ttgtggcttg taaatatcag ggttgaacct attaaaagcc cggtaaactt
     2101 tttatacttc gcaaaaaact taaacactcc cgtagcaggt gcatgtgcat atttgataac
     2161 ttcggaggat atgcccacat catgtgcagg cttgaaagac agtgtaacgg gaatcaacag
     2221 caggaacatg gcaaaatata ttatggggac cgaatccgct ccgaatttcg cagttatata
     2281 tcctacaccg aaagaggcaa aagccgcacc taacgaaccg gcagctcttg caatagtgaa
     2341 attgagctgt atgcctttgt tggcgtattc catggcaaag gaattaatga atgagtgcaa
     2401 tgtatgtacg aaggttatta tgagcaataa caatatggct gttagataag gataactcct
     2461 tataaccaca atcaaaacgg tcaatacaac aattgcagag gttagtgcca atattattct
     2521 ctttaatttc gactcttccg ctttatccgc ataggccgct ataggcggtt gtaataaggc
     2581 tgccaatatg cttgcaaccg caagtatgat tcctgtctgt gaactgttaa atcctttatc
     2641 cgaaagaaag tatgcggcat aattgtatac ggttataaag cccatccagt agagagcctg
     2701 cattattccg tac
//
LOCUS       DAOPWC010000200         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39307_length_2701_cov_3.888133, whole genome shotgun sequence.
ACCESSION   DAOPWC010000200 DAOPWC010000000
VERSION     DAOPWC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39307_length_2701_cov_3.888133"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..248)
                     /locus_tag="PKJ65_04975"
                     /pseudo
     CDS             complement(33..248)
                     /locus_tag="PKJ65_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
     gene            381..953
                     /locus_tag="PKJ65_04980"
     CDS             381..953
                     /locus_tag="PKJ65_04980"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNZ41222.1"
                     /translation="MAKLYFRYGAMGSSKSANAIMVVYNYKERNQTAVLLKPVSDNRD
                     GEQINVSRCGLSMECKYAETFLNQSDEEISKYDCIVIDEAQFLTKAQVETLVHIADDL
                     DIPVICYGLRADFQRKLFEGSLYLLAWADTIEEIKTICWCGKKATCNARIGPDGKIVR
                     EGPQFFLGGNDTYIPLCRKHHRSGQTKKED"
     gene            complement(1051..>2701)
                     /locus_tag="PKJ65_04985"
     CDS             complement(1051..>2701)
                     /locus_tag="PKJ65_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ41223.1"
                     /translation="DFFKVVEDLKEQFGNKVLPFHFPIMEDGEFKGIVDIVYKKAYMV
                     KDKVTKVMDIPAEYLAKTEELREPIMEAVAETEEELMEKYFEGEHFTDEEIIKGLTHG
                     IEEDSIFPVFCGSSMLLAGIDVLMDELISLFPSPAAYKVHAVKAGTDEQVVLNPSSDE
                     KFAGLVFKTMVDAFVGKISLIKVCSGELRSDMTVINSRTTDTEKMSNLFFIKGKKQEQ
                     TDKAVAGDIVAVAKLTDTQTNDTLSLQSFPLELKKIDFPQPSISLAVEPLAKGDEDKI
                     GSGLSKLSDEDPTFRVENNPETKQLLISGLGEQHIDVITSKLKAKYGVSVKLTDPKIP
                     YRETIKKKLETEGKHKKQSGGHGQYGHVKVIFEPGEEEDLVFEEKIFGGSVPKNYIPA
                     VEKGLRECIVKGVLAGYPMVRLKATLIDGSYHDVDSSEMAFKIASSLAYRKLIDASPV
                     LLEPIMSCEVMIPDAYLGDIIGDINKRRGRILGMHPADKGYQKVIAEVPHAEMFKYAT
                     DLRSMTQGRGSFTMKLERYEEMPGNLAEKVVAASKREGEEE"
BASE COUNT          717 a          631 c          504 g          849 t
ORIGIN      
        1 gatcctctcg gtctttaaca cttcctcagc aaaatacggc ttatggtaat gtggcctccg
       61 ccccctcttt tactttccac gaaatatcct ctttcggagt tgaatcttgt tgctatgaca
      121 tagtttatct gtgacggaac acaattaaag taaccggcaa gttcattcct ctgaatctcc
      181 acttgcccgt cggtttcatc tattatctct ttaatgaagt cctctattat atcactcaat
      241 cttgccattc attatcacct ttgactttct ctgactttat tatatcaaat gtgatatttt
      301 tttcaacctc tatttaaaac ggcactccca gatggtatac tatatatgca acctttttat
      361 atattctttc ggaggaaact atggctaaac tatatttcag atacggagcg atgggaagtt
      421 caaaatccgc aaatgcaatt atggtggttt acaactataa agaaagaaac cagaccgcag
      481 tgctgttaaa accggtatcg gataatcgtg acggcgaaca gataaacgta tccagatgcg
      541 gtttaagcat ggaatgcaaa tatgcggaaa cttttttaaa tcagtcggat gaagagataa
      601 gtaaatatga ctgtatcgtt atagacgaag cacagtttct tacaaaggca caggtggaaa
      661 cgcttgtaca tattgccgat gacctggata tacccgttat atgctacgga ttaagagcgg
      721 actttcagcg taaacttttt gaaggcagtc tatatcttct ggcatgggcc gacacaatag
      781 aggagataaa gaccatctgc tggtgcggta aaaaagccac atgcaatgca agaataggcc
      841 ccgacggtaa gattgtgcgg gaaggtccgc aattcttcct cggaggtaac gacacatata
      901 tcccgttatg cagaaagcat cacagaagcg gacagacaaa aaaggaagac taaaaggaca
      961 gattatctgt ccttttaata tatttactcc tcatagttac atcttcacac cctttcagtt
     1021 atatacgaac ataacgaaag tatgaatacc ttattcttct tcgccctctc tcttggaagc
     1081 tgcaacaact ttttccgcca agtttccggg catttcctca tatcgctcca acttcatggt
     1141 aaaggagcct ctgccctgag tcatacttct caaatcggtt gcatacttga acatttccgc
     1201 atgaggaact tcggctatta ccttctggta tcctttgtcg gcaggatgca tacctaaaat
     1261 tcttcctctt ctcttgttga tgtcaccgat tatgtcaccc aaatatgcgt cgggaatcat
     1321 tacttcacag gacataatgg gttccaacag aacgggagaa gcatcaataa gcttcctgta
     1381 tgccaagctg gaagcaattt taaatgccat ttccgaagag tcgacatcat gataggaacc
     1441 gtcaataagg gtggctttca gtcttaccat gggatatcct gcaagcactc ctttaactat
     1501 gcattccctc aatccttttt ctacggccgg tatatagttt ttaggtaccg agccgccgaa
     1561 aatcttctct tcaaatacca aatcttcttc ttcaccgggc tcaaatatta ctttaacatg
     1621 accgtactgt ccgtgacctc ccgattgttt cttatgtttt ccttcggttt ccagtttttt
     1681 ctttattgtc tctctgtacg gtattttcgg atcggtaagc ttaaccgaaa ctccgtattt
     1741 tgctttaagt ttgctggtta taacatcaat atgttgttct ccgagaccgc ttatgagaag
     1801 ctgcttggtc tccgggttat tctccactct gaatgtagga tcttcatcac tgagtttact
     1861 caatcccgaa cctatcttat cctcatcacc tttggcaaga ggctctaccg ccaatgatat
     1921 tgaaggttgg ggaaaatcaa tcttcttaag ttccagaggg aaagactgca acgacagtgt
     1981 gtcattagtc tgggtatcgg tcagttttgc tacagctact atatcacctg caacagcttt
     2041 atcggtctgc tcctgctttt tacctttgat gaaaaagagg ttggacattt tttccgtatc
     2101 cgttgttctg ctgtttataa ccgtcatatc cgacctcaat tcgcccgaac agacctttat
     2161 cagcgagatt tttcctacga aagcatccac cattgtcttg aataccagtc ctgcgaactt
     2221 ctcgtcgctt gaaggattga gcaccacctg ttcatcggtt cccgccttaa cggcatgtac
     2281 cttgtacgcg gccggtgacg ggaataacga aatcaattca tccatcagga catcaatacc
     2341 cgcaagcagc attgaagaac cgcagaatac cgggaaaatc gaatcttctt ctattccgtg
     2401 agttaagcct tttattattt cctcgtctgt gaaatgttcg ccttcgaagt atttttccat
     2461 aagctcttct tcggtttccg ctaccgcttc catgatgggt tctctgagct cttcggtttt
     2521 agccaggtac tcagccggaa tatccatgac tttggttact ttatctttaa ccatgtatgc
     2581 ttttttgtag actatatcta ctataccttt aaactcgccg tcttccatta tcgggaaatg
     2641 aaacggtaat actttgttgc cgaattgttc ttttaaatct tccacaacct taaagaagtc
     2701 c
//
LOCUS       DAOPWC010000201         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39617_length_2685_cov_3.613308, whole genome shotgun sequence.
ACCESSION   DAOPWC010000201 DAOPWC010000000
VERSION     DAOPWC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39617_length_2685_cov_3.613308"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKJ65_04990"
     CDS             complement(<1..378)
                     /locus_tag="PKJ65_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HNZ41224.1"
                     /translation="MNILVDAMGGDNAPEAIVTGCLDAISMKEGFDITLFGDGKIIAD
                     ILCNRNYDKSRIKMEHCSEVILFTDTPTAAVKNKKDSSMIRALTMMREGFGDAVISAG
                     NSGALLTGSLLITGRMDKVIRPAL"
     gene            complement(390..926)
                     /locus_tag="PKJ65_04995"
     CDS             complement(390..926)
                     /locus_tag="PKJ65_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis transcriptional
                     regulator"
                     /protein_id="HNZ41225.1"
                     /translation="MTKATLKKLKKLEDLQILLSESPYMNDKQLAEALGVSVPCIRNY
                     RNELGIKQVRERIKDTVRSLAPEDIVGTITNLKLNEGASAEFVTTADMVFKKTNIVRG
                     QYLYSFAESVAISVIDAEASLVGVANIKYKEPVYANEKLTARAEVTQIRMSNYFVWVF
                     VYREDTEIFRSKFILVSV"
     gene            complement(1052..1615)
                     /gene="pgsA"
                     /locus_tag="PKJ65_05000"
     CDS             complement(1052..1615)
                     /gene="pgsA"
                     /locus_tag="PKJ65_05000"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNZ41226.1"
                     /translation="MNIANKLTFIRIILIPFFIIFGIPAPEWLSEPVFSFHQQYGTLI
                     ALVIFVIASLTDLFDGYLARKYKLITNMGKFLDPIADKILITSALVVLVERGQIAAYT
                     VVIIIARDLIVDGLRLVVSEKGEVIAANIFGKIKTNLQIFSICIYLLAYYINLWYIPK
                     IVYALALIATVYSGIIYVKQSIKFIKD"
     gene            complement(1615..>2685)
                     /gene="rimO"
                     /locus_tag="PKJ65_05005"
     CDS             complement(1615..>2685)
                     /gene="rimO"
                     /locus_tag="PKJ65_05005"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ41227.1"
                     /translation="ERYKQEIITSIPEVDVCVGIDGYKDIASVVGKTDKKLICGNKTD
                     VGFMNIERLLSEHTASTYVKIAEGCDNRCTYCTIPSIRGRYRSRSIEDIYTEAEKLCS
                     LHGIKEITLVAQDTTAYGTDIYGKPSLDLLLKKLETVDSLQWIRLLYCYPELITDELI
                     TTVKNSKKILHYLDIPLQHSSDRILKLMGRKGTGAMYRELTDKLRFHIPDIVLRTTFI
                     VGFPQETEEDFNDLCGFIKASRFERAGFFEYCKEEGTPAYGLKGQISKKIKMQRRKHA
                     EQIQQDILISKNDERIGKTYSIIIQGISDDGLFYAGRSYAEAYEIDPVIYAASKQELS
                     VGDMVDAVIVAKHQDSLIAEVL"
BASE COUNT          775 a          620 c          424 g          866 t
ORIGIN      
        1 caaagccgga cgtattactt tatccatcct gcccgttatg agcaaagaac ctgtaagcaa
       61 tgcaccggaa ttaccggcag atattaccgc atctccgaac ccttcacgca tcatcgtaag
      121 agctcttatc atggaagaat cctttttgtt tttaacagcc gcagtgggtg tatcggtaaa
      181 aagtattact tccgagcaat gttccatttt aattctcgat ttatcatagt tacggttaca
      241 aagtatatcg gcaataatct taccgtctcc gaataaggtt atatcaaaac cttctttcat
      301 gcttatcgca tccaaacatc ctgttactat tgcttccgga gcattgtcac cgcccatggc
      361 gtctacaagt atattcatat atcctcacat cataccgaaa ctaaaataaa tttgctacgg
      421 aaaatttccg tatcttctct gtaaacaaac acccatacaa aatagttgct cattcttatc
      481 tgtgtaactt ccgcccgtgc ggtaagtttt tcattggcat ataccggctc cttgtattta
      541 atatttgcca cacctaccag agaagcttcg gcatctataa cggaaatagc taccgattcc
      601 gcaaaggaat aaaggtactg acctctgact atattggttt ttttaaaaac catatcggcg
      661 gttgtaacaa attcggcaga agctccttca ttaagtttca gatttgttat tgttcccact
      721 atgtcttcag gtgccagcga ccttacagtg tcttttattc tttcacgaac ctgctttata
      781 cccagttcat ttctgtaatt tcttatacac ggtaccgaaa ctcccaaagc ttcggcaagc
      841 tgcttatcgt tcatatacgg actttccgaa agcaatatct gcaaatcttc aagtttcttt
      901 agttttttca gtgtcgcctt tgtcatgctg ttcccttcaa aacattttta ttacctgata
      961 acaaatttac ataagtatat tccatcacac atattttgta aagagatata cattattttt
     1021 ccttaacttt tctcaaaaaa tagatgaaaa atcaatcctt tatgaactta attgactgct
     1081 taacatatat gattcccgaa tatacggtag ctatcagagc caatgcgtaa acaattttcg
     1141 gtatatacca aagattgata tagtaagcga gaagatatat acatatgctg aatatctgaa
     1201 gattggtttt aatcttaccg aatatattag ccgctattac ttcacccttt tcacttacca
     1261 ccagccgcaa tccgtccaca atcaaatctc tggcaataat aatcacaacg gtatatgccg
     1321 ctatctgtcc tctttccacc agtacaacca gtgcacttgt aataagaatc ttatcggcaa
     1381 tgggatctaa aaatttaccc atatttgtta tcagtttgta cttcctcgcc aaatatccgt
     1441 caaaaagatc ggttaaggat gctataacga aaattaccaa cgcaattaat gtaccgtact
     1501 gctgatgaaa agaaaaaacc ggctcactca accattccgg cgcgggaatg ccaaagataa
     1561 tgaaaaaagg tatcaatatt attcttataa atgtaagttt attcgctata ttcattacag
     1621 aacctccgca atcagtgagt cttgatgttt ggcaacaatt acggcatcta ccatatcgcc
     1681 gactgataac tcctgtttac ttgcagcata tatgacggga tctatttcat atgcctccgc
     1741 ataagacctt cctgcataga acaatccgtc atcggaaatg ccttgtataa ttatactata
     1801 tgttttgcca atcctttcat catttttact gattaatata tcctgttgaa tttgttccgc
     1861 atgtttgcgt ctttgcatct ttatcttttt agatatctgt cctttaagtc cgtatgcggg
     1921 agtgccttct tctttgcagt attcaaagaa acccgccctt tcaaaacggg aagcctttat
     1981 aaacccgcac aaatcattaa aatcttcctc cgtctcctgc ggaaacccca caataaaagt
     2041 ggttcttaac acaatatccg gtatgtgaaa gcgcagttta tcagtcaact ccctatacat
     2101 ggcaccggtt cccttacgcc ccataagttt cagtattctg tcggaagaat gctgcagggg
     2161 aatatccagg taatgcagta tcttcttact gttcttaacg gtagttataa gttcatccgt
     2221 gataagttcg ggatagcagt acagtaatct tatccattgc aaagaatcga cggtttccaa
     2281 ctttttaagc aacaaatcca aagaaggttt accgtaaata tctgttccgt atgcggtagt
     2341 atcctgagcc acaagtgtta tctctttaat accgtgcaag gagcacaact tttccgcttc
     2401 cgtatatata tcctcaatac ttctgctcct gtatcttccc cgaatggacg gaatggtgca
     2461 gtaagtacat ctgttatcac agccttccgc aattttcaca tatgtcgatg cggtatgttc
     2521 cgacagcagt cgctctatat tcataaaacc gacatcggtt ttattgccgc atatcaattt
     2581 tttatccgtt ttaccgacaa cagatgctat gtccttatag ccgtctattc ccacacatac
     2641 atccacttcg ggtattgaag ttattatctc ctgcttatat ctttc
//
LOCUS       DAOPWC010000202         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39742_length_2679_cov_4.184832, whole genome shotgun sequence.
ACCESSION   DAOPWC010000202 DAOPWC010000000
VERSION     DAOPWC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39742_length_2679_cov_4.184832"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKJ65_05010"
     CDS             complement(<1..688)
                     /locus_tag="PKJ65_05010"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNZ41228.1"
                     /translation="MRKTNIVKAFIIIALVLLIIAYTPAVLWWTGKVSLPVFFTYMRT
                     VIHVGLLVAWAMSVYYRIIHKQVRRYLISIGGLMIFWLLIKTLKYYFVYDVDSTRMLW
                     YAYYIPLLLIPTMGVLVALSLGKPENYRLPKWTNYFFICAVILVLLVLTNDFHNLVFI
                     FPKGETGSDKNAAFNIIFLVVMAWVNLVGTVAIILIILKCRIPKSRAVLWLPLVSLSL
                     TILYGILYLIK"
     gene            complement(1177..1500)
                     /locus_tag="PKJ65_05015"
     CDS             complement(1177..1500)
                     /locus_tag="PKJ65_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ41229.1"
                     /translation="MIVKYSVNYSDEALDDLRDIYSYISESLMEPTIAAGQVNRIRTE
                     IKALDRLPFRHSVVDWEPWHTMEMRHFPVDNFVVFYLVEDKSVNVVRIFYGRRDIESI
                     IKNSK"
     gene            complement(1497..1802)
                     /locus_tag="PKJ65_05020"
     CDS             complement(1497..1802)
                     /locus_tag="PKJ65_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNZ41230.1"
                     /translation="MANTATVYARIDPELKSDVDSILNKLGVTPSALIQMLYSQIKLT
                     ESIPFEIKIPAKIPLSLGSMSVERLNTELQKGVDSVKAGRVHSADEVDGILNKEFGI"
     gene            complement(1974..2048)
                     /locus_tag="PKJ65_05025"
     tRNA            complement(1974..2048)
                     /locus_tag="PKJ65_05025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2013..2015),aa:Arg,seq:ccg)
     gene            complement(2054..>2679)
                     /locus_tag="PKJ65_05030"
     CDS             complement(2054..>2679)
                     /locus_tag="PKJ65_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41231.1"
                     /translation="DKLHLFMERMTTSVIKGVEQTNKLKIYDTNANTCHCSYVYTDEL
                     LPDSGMGKESITQNGWAFGLAQLFSSVSPAVTEEFEIPYVTRMAKYFGMIYYGCCDRL
                     DDRLEMVLTIPNLMKVSCSPWSDRDAFAEKIGKKVVMSNKPTPAFVATTTMDEEEIRK
                     DLQWTVDAARKNNVNLEMILKDISTVKCQPERLTRWADIAMDVVNNY"
BASE COUNT          803 a          589 c          492 g          795 t
ORIGIN      
        1 attttatcaa atataaaatc ccatatagga tggtgagcga cagtgaaacc aacggtaacc
       61 ataagacagc ccgactttta ggaatgcgac acttgagaat aatgagaata atagcgactg
      121 tgcctacaag attcacccac gccataacga ccaaaaatat tatgttgaat gcagcatttt
      181 tgtctgaccc cgtctctcct ttcggaaaaa taaaaaccaa attgtgaaaa tcgtttgtga
      241 gcaccaataa aaccaaaatc acagcacaga taaaaaaata gttcgtccac ttcggaagac
      301 gataattttc cggcttacca agcgacaacg caaccaaaac acccatagtg ggaatgagaa
      361 gcagcggaat atagtaggca taccaaagca tacgggtcga atctacatca tatacgaaat
      421 agtatttcag tgttttaatt aataaccaaa aaatcatcag accgcctatg gaaataaggt
      481 atctcctaac ctgtttatga ataatgcgat agtaaaccga catagcccat gcgacgagaa
      541 gaccgacatg aataactgta cgcatgtatg taaaaaagac aggtaaagaa accttgcctg
      601 tccaccagag gacagcggga gtatatgcaa taataagtaa tacgagagca atgataataa
      661 aggcttttac tatatttgtt tttctcatat caacacatcc gtcgttgttt tgctgataca
      721 attatacacg atagtttacc gtaagtgaaa actttcatta caaagattgc gttgtgctaa
      781 tattaagaaa acttggcact acacttgtgc aagaacggat ttaaagaacg ccacataacg
      841 ccgtatctct tcttcgattc cgaattgtct gccgtaattt ataagttttc ttaaatcttt
      901 gctttttcct ttgaaatata cggaaagtgt accgacaaaa actgtgtatc ggctttttct
      961 ctatcaagaa cgatgtcgca aatgcttctt tctacatcat aaacttgaac tacatttccg
     1021 agcggtgttc gtatttccga tataccgata ggatataagt ctttttaata tagtgaatct
     1081 tcaggtttgc atatctcttt ttgatgtgga cgataatgaa ccgtaatcgt gactcctatc
     1141 aaacgaagtt gcactgcgat atgaaagaca gataacctat ttggaattct ttataattga
     1201 ttcaatatcc cgtctgccgt aaaaaatgcg aacaacattt acgcttttat cttccaccag
     1261 ataaaaaaca acgaagttgt ctaccggaaa gtgacgcatt tccattgtat gccatggttc
     1321 ccaatcaaca acactgtgac gaaacggcag tctgtcaagt gctttaattt cagtacggat
     1381 acggttaacc tgtccggcag caatggtcgg ttccataaga gattcggata tatagctata
     1441 aatatcccga agatcgtcca gtgcttcatc ggaatagttt acagaatact tcactatcag
     1501 ataccgaact ccttattcag tattccgtca acctcatccg cagagtgtac acgaccggct
     1561 ttcaccgagt caactccttt ctgaagttca gtgttcaacc gttcaacaga catgcttccg
     1621 agtgaaagcg gaatcttagc cggaatttta atctcaaacg gaatgctttc tgtcagtttg
     1681 atttggctat agagcatttg gataagtgcg gacggagtta ctccaagctt attaagaatg
     1741 gaatcgacat ccgatttgag ttcgggatca attcttgcat atacggtagc tgtatttgcc
     1801 attggaaaaa cctccttatg tttatctggt tgtagtatgt cacaaatttt tgcagcttgc
     1861 aagcatttag catacatttg caaaatttta tgaccatttt tcactccaaa acagtccgat
     1921 gattgcctaa aacacaagaa gaaacgtctt ttaggcgata gacgtttctt ttatggcgtc
     1981 ccgaacagga atcgaacccg catctgagga accggaatcc cccgttttat ccattatact
     2041 accgggacaa tctttagtag ttatttacca catccattgc tatgtcagcc caccttgtca
     2101 gtctttccgg ttgacatttc accgtggata tatccttcag tatcatttcc agatttacat
     2161 tgttctttct tgccgcatct accgtccact gaagatcctt ccttatctcc tcttcatcca
     2221 tcgtggttgt ggcgacaaat gcaggagtgg gcttgttgct cattaccact tttttgccta
     2281 tcttttctgc aaatgcatct ctgtccgacc agggactgca ggatactttc attaagttgg
     2341 gtattgtcag taccatctcc agcctgtcat ccaatcggtc acagcatccg tagtatatca
     2401 taccgaagta ttttgccatc cttgttacat agggaatctc gaactcttcg gtcactgccg
     2461 gtgataccga agagaacagc tgtgcaagac cgaatgccca accgttttgc gtaatgcttt
     2521 ctttacccat tccgctgtca ggcagcaatt catccgtata cacataggag caatggcagg
     2581 tatttgcgtt tgtatcatat atcttaagct tatttgtctg ctcaactccc tttataaccg
     2641 aagtggtcat tctctccata aaaagatgaa gcttgtccg
//
LOCUS       DAOPWC010000203         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39758_length_2678_cov_3.024781, whole genome shotgun sequence.
ACCESSION   DAOPWC010000203 DAOPWC010000000
VERSION     DAOPWC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39758_length_2678_cov_3.024781"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKJ65_05035"
     CDS             <1..762
                     /locus_tag="PKJ65_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ41232.1"
                     /translation="IATPVFDGASEMDIIECLKMANLSEDGKTVLYDGRTGEPFDNRV
                     TVGYMYMLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTVKSDDVIGRVKTYEAIVKGENIPKSGIPESFKVLVKELQSLALDVKILK
                     SDHSVVMLRDGSEDDIPRLDINISGTEDYTLDNDELFNMELARGDDDFEDDEDINSEE
                     ISGMMMKTADDNDTGVDYSKISDVQDIVDLDDMGE"
     gene            766..>2678
                     /gene="rpoC"
                     /locus_tag="PKJ65_05040"
     CDS             766..>2678
                     /gene="rpoC"
                     /locus_tag="PKJ65_05040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ41233.1"
                     /translation="MQDYTNFDMIKIGLASSEMIKEWSRGEVKKPETINYRTLKPEAG
                     GLFCEKIFGPTKDWECHCGKYKRVRHKGIICDRCGVEITKSKVRRERMGHIELAAPVS
                     HIWYFRGIPSRMGLVLDMSPRALERILYFNSYVVLEVHNEDVKVNDKTIEVKDLLTEL
                     QYRELIDKYGRDSFKARMGAEAVQILLARIDVEVESTLLKEELQTSTGQKRGRIIKRL
                     EAMESFRVSGNKPEWMILDTIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRL
                     KRLLELTAPDIIVRNEKRMLQEAVDALINNGRKGKPVVGPGNRPLKSLSDMLRGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELMMYQCGLPKEMALELFKPFVMKRLVTDKFAQNIK
                     SAKKMVERVRNEVWDILEDVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLV
                     CTAYNADFDGDQMAVHVPLSAEAQSEARILMLSANNLLAPKDGKPITVPTQDMVLGGY
                     YLTTAKDGEIGEGRVFRNFDEMLMALHEKTISLHSKVKMRVEREVDGKVVSKIIDVTC
                     GKVIFNEAIPQDLGFVDRTNPDTMFDLEIDRMVGKKNLSEIIERCITKHGIAKTAEVL
                     DKIKSIGFRYSTQG"
BASE COUNT          869 a          494 c          667 g          648 t
ORIGIN      
        1 atagccaccc cggtattcga cggagcatcg gaaatggaca taatagagtg tctgaagatg
       61 gcaaacctgt cggaagacgg caaaaccgtt ctgtatgacg gaagaaccgg cgagcctttt
      121 gataacagag ttaccgtggg ctatatgtat atgcttaaac tgcatcacct tgttgatgat
      181 aagattcatg cccgctccac aggaccgtat tcgttggtta cgcaacagcc tttaggcggt
      241 aaagcacagt tcggcggaca gcgtttcgga gaaatggaag tgtgggcact ggaagcatac
      301 ggcgccgcct atactttaca ggagatattg accgtcaaat ccgatgatgt tatcggaaga
      361 gtaaagacat acgaggcaat tgtaaaggga gaaaatatcc ccaaatccgg tattccggaa
      421 tcttttaaag ttctggtcaa ggaacttcag agtcttgctt tggatgtgaa aatactcaag
      481 tcggatcact cggtagttat gcttcgtgac ggaagtgaag atgatatacc cagattggat
      541 atcaatattt ccggaaccga agattataca ttggacaatg atgaattgtt caatatggaa
      601 ttggcaagag gcgatgacga ttttgaggac gacgaagata tcaattcaga ggaaatatcc
      661 ggtatgatga tgaaaactgc tgatgacaat gacaccggcg tagattattc aaagatatcc
      721 gatgtgcagg atatagttga tttagatgac atgggagagt aaagaatgca ggattataca
      781 aattttgaca tgattaaaat cggtttggct tcttccgaga tgattaagga atggtccaga
      841 ggcgaggtta agaaacctga aacaattaac tacagaacac tgaaacctga agccggcgga
      901 cttttctgtg aaaaaatatt cggtcccacc aaggactggg aatgccattg cggaaagtat
      961 aaaagagtca gacacaaagg tataatatgt gaccgttgcg gagtggaaat taccaaaagc
     1021 aaggtaagaa gagagcgtat gggacatata gaacttgcgg cacctgtctc ccacatatgg
     1081 tatttcagag gaattcccag cagaatgggt cttgtattgg atatgagccc cagagcattg
     1141 gaaaggattc tgtattttaa ctcttatgta gtgctggaag tgcataatga agatgtcaag
     1201 gttaatgaca aaaccattga agtaaaagat ttacttaccg aacttcagta cagagaatta
     1261 atagataaat acggcagaga ttctttcaaa gcacgtatgg gtgccgaagc ggtgcagata
     1321 ttgcttgcaa ggattgatgt tgaagtggaa agtactcttc ttaaagagga actgcagaca
     1381 agcacgggac aaaaaagagg aagaataatc aagagactgg aagctatgga atcattcaga
     1441 gtgtccggaa acaagccgga atggatgatt ttggacacca ttcccgttat acctcccgat
     1501 cttcgcccta tggtgcagtt ggacggcggc agatttgcca cttccgacct taatgacctt
     1561 tacagaagag ttattaacag aaataacagg cttaagaggc ttttggagct taccgctccg
     1621 gatattatag tcagaaatga aaaaaggatg cttcaggaag ctgtcgatgc cctcataaac
     1681 aacggcagaa aaggaaaacc cgtggtagga ccgggcaaca ggccgctaaa atccttatcg
     1741 gatatgttaa gaggaaaaca gggccgattc agacagaacc ttttgggtaa gagagtagac
     1801 tattccggac gttcggttat agttgtggga ccggaactga tgatgtatca atgcggtctt
     1861 cccaaagaaa tggcactgga gcttttcaaa ccctttgtca tgaaacgtct tgttaccgat
     1921 aagttcgctc agaatattaa gagtgcaaag aaaatggtgg aaagagtacg caatgaagta
     1981 tgggatattc ttgaggatgt tattaaagat caccccgttc ttctaaacag agcacctact
     2041 ctgcacagat tgggaataca ggcgtttgaa ccggttcttg tagagggaag agctatcaaa
     2101 ctgcatccgt tggtatgtac cgcatacaat gcggactttg acggagacca gatggctgtt
     2161 cacgttcctc tttctgcaga ggctcagtcg gaagcaagaa tactaatgct ttccgccaac
     2221 aatctgcttg cacccaaaga cggaaaaccc attaccgttc ccactcagga catggttctg
     2281 ggaggttact atcttaccac cgcgaaagac ggagagatag gcgaaggaag agtattccga
     2341 aactttgacg aaatgcttat ggcattgcat gaaaagacaa taagcctcca ttcgaaagta
     2401 aaaatgagag tggaaagaga agtagacgga aaagttgtaa gcaaaataat tgatgtaacc
     2461 tgcggtaaag ttatattcaa tgaagctatt cctcaggacc tgggttttgt ggacaggacc
     2521 aatcccgata ccatgttcga tttggaaata gaccggatgg tgggtaaaaa gaatctttcg
     2581 gaaatcattg aaagatgcat aacaaaacac ggtattgcaa aaaccgccga agttctggat
     2641 aagataaaat ccatcggatt cagatattcc acacaggg
//
LOCUS       DAOPWC010000204         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_39970_length_2668_cov_3.323000, whole genome shotgun sequence.
ACCESSION   DAOPWC010000204 DAOPWC010000000
VERSION     DAOPWC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39970_length_2668_cov_3.323000"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKJ65_05045"
     CDS             complement(<1..617)
                     /locus_tag="PKJ65_05045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ41234.1"
                     /translation="MRLQKYIALCGYASRRAAEELIFNGSVSVNGKIVRDIVMVDDSD
                     EVRVNGKLLKTETKKLYFVFNKPEMVITSMSDKFGRKTVADFFNNIEERVYPVGRLDY
                     DSSGLLFMTNDGDFADYLMHPKNEVTKTYAVSAKGNITDEIKEMLENGVDIGGYITGK
                     AEINNIVNYSDGKCSAEISIKEGKNRQVRKMFETVGIKVYALQRIS"
     gene            complement(635..1198)
                     /gene="scpB"
                     /locus_tag="PKJ65_05050"
     CDS             complement(635..1198)
                     /gene="scpB"
                     /locus_tag="PKJ65_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNZ41235.1"
                     /translation="MDKEYIINQITAMLYANGEPVPLTRLEQILETDKKEIKKIIDEH
                     IEHLKDIKSGVLIRQIEDSYQMCTNPIYFPAVRQLFEKKKTDTLSRAAYETLAIIAYN
                     KVATRAKIESIRGNSDSPLKTLLERGLVIEAGRLEAPGRPVLYKPAEEFYRAFDVSSL
                     DDLIPLDSFDDEEVTDTEETEVTEVSE"
     gene            complement(1202..1969)
                     /locus_tag="PKJ65_05055"
     CDS             complement(1202..1969)
                     /locus_tag="PKJ65_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNZ41236.1"
                     /translation="MGVGYQLKLENFEGPLDLLLHLIDENKMSIYDIKISIITEQYLD
                     YLMAMQQLDMDIASDFLVIAATLLHIKSRSMLPKAKDDEIDSETDLIRQLIEYKMIKK
                     AGERFRELHEEGQKSFHSFASTEDFGESEKTYTLCKSMLVMIYRETMQRRIDKTNLKA
                     ENIKEIVKKEKISLIKSIRKIIDVLFKEKRISFFTRFKDNSVSNAQLVTDFLSILVLS
                     TQKKATLKQKKAFEDIIVEGTETLSYPDNIQEIYDWE"
     gene            complement(1960..>2668)
                     /gene="trpS"
                     /locus_tag="PKJ65_05060"
     CDS             complement(1960..>2668)
                     /gene="trpS"
                     /locus_tag="PKJ65_05060"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ41237.1"
                     /translation="LLSMITPLSWLERCSTYKDQLSQLAEKDIRTYGFLGYPCLMAAD
                     ILIYRAEGVPVGEDQLAHLEITREIARRFNYLYGEVFPEPKALLTKAKVLPGLDGRKM
                     SKSYDNTILLKEEDDVLRAKIMSMVTDPARIRKTDPGHPEICSVYAFHKVFNQMEVAS
                     ICQSCKKGEIGCVACKKNLYGKVSEFLRPIQERRKEIEAKPKLMNEILEKGNANAKIK
                     TEETMTMVRKAMKVNWE"
BASE COUNT          651 a          639 c          436 g          942 t
ORIGIN      
        1 gatattcttt gcaacgcata tacctttatg ccgacagtct caaacatttt tcttacctga
       61 cggtttttac cttcttttat cgatatttcg gcagagcatt tcccgtcact ataatttact
      121 atattattta tttcagcttt ccctgttata tatcccccta tatccacccc gttttccaac
      181 atttctttta tctcatcggt tatattaccc tttgccgaaa cggcataagt ctttgttacc
      241 tcatttttag gatgcatcag ataatcggca aagtctccgt cattggtcat gaataggaga
      301 ccggaggaat cgtaatccaa tcttcctacg ggataaaccc gctcttctat attattaaaa
      361 aaatccgcaa cggttttcct gccgaactta tccgacatgg aggttataac catctccggt
      421 ttattgaata cgaaatacag tttttttgtt tctgtcttta atagttttcc gttaaccctt
      481 acttcatcgg aatcgtccac cattacaata tcccgtacaa tcttgccgtt taccgatacc
      541 gagccgttga agataagctc ctcggccgct cttctggatg cataaccgca caatgcaata
      601 tatttttgta aacgcatttt ttccacggat tcttttattc cgacacttcg gttacttcag
      661 tttcctctgt atccgtcact tcctcatcat caaaactgtc cagcggaatc aaatcatcca
      721 aagaagacac atcaaaggct ctgtagaact cttctgcggg cttgtataac accggtcttc
      781 ccggagcttc cagtcttccc gcttcgatta ccagacctct ttccaataat gtctttaacg
      841 gactgtcgga attacctctt atactttcta tctttgctct ggtagccact ttgttgtatg
      901 cgattattgc cagagtctca tatgccgctc tggataatgt atcggtcttc ttcttttcaa
      961 acaactgacg gactgcggga aaatatatcg ggttggtaca catctggtag gagtcttcaa
     1021 tttgtcttat gaggacaccg gatttgatat ctttaagatg ctctatatgt tcatcaatta
     1081 tcttttttat ctctttttta tcggtctcca gtatctgctc caatctggtt aagggcaccg
     1141 gctccccgtt tgcataaagc attgcggtta tctggtttat tatgtactct ttatccatat
     1201 cttactccca atcgtatatt tcctgaatat tatccggata gcttaatgtt tcggttcctt
     1261 ccacgattat atcttcaaaa gctttttttt gcttaagtgt ggcttttttc tgtgtcgata
     1321 aaactaaaat ggacaaaaaa tccgttacca gctgggcatt ggataccgag ttatctttaa
     1381 aacgggtaaa aaagcttatc ctcttttcct taaaaagcac atctattatc tttctgatgg
     1441 attttatgag ggatattttc tcttttttta caatctcttt tatattctcc gctttcagat
     1501 ttgttttatc aatccgcctt tgcatggttt ctctgtaaat cattaccagc atgcttttgc
     1561 aaagtgtata ggttttttcc gattctccga aatcttccgt ggaagcaaag gaatgaaaac
     1621 tcttctgccc ttcttcatgt aattctctga accgttctcc cgctttcttt atcatcttat
     1681 actcgataag ctgtcttatc aaatcggtct cgctgtcaat ctcatcatct tttgccttag
     1741 gaagcatgga tctggatttt atatgcaaca atgtggcggc aataaccaga aaatcgcttg
     1801 ctatatccat atccagctgt tgcatagcca taagataatc cagatactgt tcggtaatta
     1861 tacttatttt tatgtcgtaa atactcatct tattctcatc gataagatgt aacagaagat
     1921 ccagcgggcc ttcaaagttt tccagtttta actgatatcc tactcccaat ttaccttcat
     1981 ggcctttctt accatagtca tggtttcctc cgtctttatt ttagcattgg cattgccctt
     2041 ctccaatatc tcattcataa gcttgggttt tgcctctatc tcttttcttc tctcctgaat
     2101 cggacgtaag aactccgata ccttgccgta taagttcttc ttgcaggcaa cacatcctat
     2161 ctcgcctttt ttacacgatt ggcagattga agcaacttcc atctggttaa agaccttatg
     2221 gaaagcatat accgagcata tttccggatg tcccggatcg gtctttctta tacgtgcggg
     2281 atcggtaacc atgctcatga ttttagccct caatacatca tcttcctctt tgagtaatat
     2341 ggtattatca taacttttac tcatttttct tccgtccaac cccggcagca cctttgcttt
     2401 tgtaagcaga gctttaggtt cggggaatac ctcaccgtac agatagttga atcttcttgc
     2461 tatttctctg gttatttcca gatgcgcaag ctggtcttca cctaccggta caccctccgc
     2521 ccggtaaatg agaatatccg cagccatcag acagggataa cctaaaaaac cgtacgtcct
     2581 tatatccttc tcggcaagct ggctcaactg atctttgtat gtagaacacc gctccagcca
     2641 ggataacggt gtaatcatgg ataaaagc
//
LOCUS       DAOPWC010000205         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_40060_length_2664_cov_3.040629, whole genome shotgun sequence.
ACCESSION   DAOPWC010000205 DAOPWC010000000
VERSION     DAOPWC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40060_length_2664_cov_3.040629"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..640)
                     /gene="coaE"
                     /locus_tag="PKJ65_05065"
     CDS             complement(68..640)
                     /gene="coaE"
                     /locus_tag="PKJ65_05065"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ41238.1"
                     /translation="MIIGVTGRIATGKTSLAKAFVQRGYAVFDADETYKHILETSLTM
                     QKELLNEFKTLDRLVILSEVIDDKKSLERLNEITHKYVTERIYQFISENSGKNIVLDV
                     PVPVEKGFIDISDIIIVTVASEDVQISRLMQRYSIDKEQALRRIKMQKSEADYLSIGD
                     IILNTDKTDQGDLMTFIDMFENTYIKSVKG"
     gene            complement(645..>2664)
                     /gene="polA"
                     /locus_tag="PKJ65_05070"
     CDS             complement(645..>2664)
                     /gene="polA"
                     /locus_tag="PKJ65_05070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ41239.1"
                     /translation="PSEFIDVKALMGDKSDNIPGVQGIGKVGALNLIKEYKDIDNLYQ
                     NIDNIDKPALKEKLIKDKYNAFLSKELAKIDTDVPLDDLKTDNLKIKPFNNQQLLKLF
                     IKLEFNSFIQKFQLEEKDENINETAQQFNEVSDLKDFDKTGSKLFLYYKLDENILEIE
                     IMNEKHQILKAFDKEAYICLKEIFLSDLTIVSHYLKDLFVYCFYNNIKIPDKYYDTAT
                     AMYLLDSMKEKYDIDDTYSNHNISGSVNDPKPYKIKYLQKYSENEIKTTDMQSLFYDI
                     ELPLVEILAYMEYVGIRADRDYLFELSEYFDNEINITAKKIFDYAGCTFNINSPKQLG
                     IVLFEQLKLPHAKPNSSGGYSTSAEVLEKLKRYPIVEEILYYRTVSKLYSTYAQGLLS
                     AILIDGRIHSKFNQTVTATGRISSTEPNMQNIPVRTEIGRKIRKAFIPEEGYTMISGD
                     YSQIELRILAHIADDENMRNAFLNKEDIHASTAAKVFGVNLADVTKEQRYAAKAVNFG
                     IIYGISDFSLSKDINVPVKKAAKYIEDYLNHYSGVKEYMQKIVRSAMEDGYVSTIYGR
                     RRYIPELSSPKFQIREFGKRVALNAPIQGSAADIIKIAMIRVYKQLKEQNLKSRLILQ
                     VHDELVIETHKQEIEQVKEILQQSMEKGFDLSVPLDIEISVQDSLLKG"
BASE COUNT          743 a          515 c          435 g          971 t
ORIGIN      
        1 tttttgccat agttccacct tttctttttt tattcgaata gaattttaac attcggcatg
       61 ttaaaaatca acccttaacg gactttatgt aagtattttc aaacatatct atgaatgtca
      121 ttaaatcgcc ttgatcggtt ttatcggtat ttaatattat atcgccaatt gagagataat
      181 ctgcttctga tttctgcatt tttatccttc tcaatgcttg ctctttatct atactatatc
      241 gttgcataag tcgcgatatc tgaacatctt ctgaagcaac tgtcactata ataatatcgc
      301 ttatatcaat aaaccctttt tccacgggta cgggcacatc cagaacgatg ttttttccgg
      361 aattttcact aataaactga tatattctct ctgtaacata cttatgtgta atctcattaa
      421 gtctttccag tgatttcttg tcgtcaatta cttcagaaag aattaccaat ctgtcaaggg
      481 ttttgaactc gttaagtaat tctttttgca tagtaagaga agtttccaga atatgcttat
      541 aggtttcatc ggcatcaaaa accgcataac ctctttggac aaatgctttt gccaaggaag
      601 ttttacctgt agcgatacgg cctgtaaccc ctataatcat ctttctatcc tttcaaaaga
      661 gaatcctgaa cggatatttc tatatccaaa ggtaccgata aatcaaaacc tttttccatg
      721 gattgttgta aaatttcttt tacctgctca atttcctgtt tatgtgtttc aataaccagt
      781 tcgtcatgta cctgaagtat aaggcgggat ttcaggtttt gctctttcaa ttgtttatat
      841 actcttatca tggcaatctt tattatatcc gcagcagaac cctgtatggg ggcattcagg
      901 gctactctct tgccgaactc ccttatttga aatttaggtg aactgagttc cggtatatat
      961 cgtcttcgtc cgtatattgt ggaaacatat ccgtcctcca tagccgaacg gactattttt
     1021 tgcatgtact cttttactcc cgaataatga ttgagataat cttctatata tttagcggct
     1081 tttttaaccg gtacgttgat atccttggat aaactgaaat cggatattcc gtaaatgata
     1141 ccgaaattaa cggctttggc ggcatatctt tgttcttttg taacatcggc cagattcact
     1201 ccgaacacct tggcggcagt ggaagcatga atatcttctt tgttgagaaa tgcatttctc
     1261 atattttcat catcggcgat atgcgcaagt atacgtaatt ctatctgtga atagtcaccg
     1321 cttatcattg tatacccctc ttcaggtata aaggctttac gtatctttct ccctatttcc
     1381 gttcttacgg gtatattctg catattgggt tcggttgagc ttattcttcc ggttgcggtt
     1441 accgtctgat tgaatttact gtgtattctt ccgtcaatca aaatggcgga caacagacct
     1501 tgtgcataag tggaatacag ttttgaaacg gtgcgataat acagtatctc ttctacaata
     1561 ggatatcgct tgagcttctc taaaacttcg gcggatgtgg aatagccgcc cgatgaattg
     1621 ggtttggcat gcggcaactt caattgctca aataatacaa ttcccagttg tttgggagaa
     1681 ttgatattaa aagtgcaacc ggcataatcg aatattttct ttgccgttat gttaatttca
     1741 ttatcgaaat attccgaaag ttcaaataag tagtctctgt ccgcccgtat accgacatat
     1801 tccatatatg ccaatatctc cactaacggt aactctatgt cgtaaaacag cgattgcata
     1861 tccgttgtct ttatctcatt ctcggaatac ttttgaagat actttatctt ataaggtttg
     1921 ggatcgttga ccgagccgga tatattatga ttgctgtagg tatcatcgat atcatacttc
     1981 tctttcatgg aatcgagaag atacatagcg gttgcggtat catagtattt gtcaggtatt
     2041 ttaatattat tgtaaaaaca ataaacgaaa aggtctttaa gataatgaga aacaatagtc
     2101 aaatccgata agaatatttc tttaaggcag atatatgctt ctttatcaaa agcctttaaa
     2161 atctgatgct tttcgttcat tatctctatt tctaaaatgt tttcatcaag cttatagtac
     2221 agaaacaatt ttgaccctgt tttatcaaaa tcctttaagt cggaaacttc attaaattgc
     2281 tgtgcagtct catttatatt ctcatctttt tcttccaact gaaacttctg aataaatgaa
     2341 ttgaattcca gtttaataaa caacttaaga agttgctgat tgttaaacgg ctttatcttc
     2401 agattatccg tttttaaatc atcaagcggt acatcagtgt ctatttttgc aagttcctta
     2461 cttaaaaatg cattgtattt atccttgata agtttctctt tcagagcggg tttgtcgata
     2521 ttgtcgatgt tttgatataa attatctata tctttatact ctttgataag atttaaagca
     2581 cccactttgc ctataccctg aactcccggt atattatccg atttatcccc catcaatgct
     2641 ttgacatcaa taaattccga aggt
//
LOCUS       DAOPWC010000206         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_40296_length_2653_cov_3.538876, whole genome shotgun sequence.
ACCESSION   DAOPWC010000206 DAOPWC010000000
VERSION     DAOPWC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40296_length_2653_cov_3.538876"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="cdaA"
                     /locus_tag="PKJ65_05075"
     CDS             complement(<1..826)
                     /gene="cdaA"
                     /locus_tag="PKJ65_05075"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNZ41240.1"
                     /translation="MFNQIDWVEQIKYFSLELSSPFAVISALIDIAIVSFLVYKLMIF
                     VKEIRAWSLLKGLLVIVFVAIVSNLLGLQIMTFIFNNIITFAALSVVVLFQPEIRKVL
                     ERLGTSEITHFFSSTGSDETLMITTVVDEIVKACESMSDRYTGALIVIEKSTKIGEFI
                     NTGIQLNADVSKEILENIFVPKTPLHDGAVIIRKDKIVAAACLLPLTDNPNLSTELGM
                     RHRGGLGITEVSDCIAVIVSEESGRISYASNGGLVRNLSPDSLRKALLMNLVREKRD"
     gene            complement(938..2572)
                     /locus_tag="PKJ65_05080"
     CDS             complement(938..2572)
                     /locus_tag="PKJ65_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNZ41241.1"
                     /translation="MPITEILEKNAGLYPDDVSLVEINPELQEKRQVTWKEFELIETN
                     PKEHYRKEITWKEFDDKANRFANFLMANGITKGKKVAILLMNCIEWLPIYFGILKTGA
                     LVVPLNYRYTAEEIRYCLNLSESNALIFGYEFISRIESIKEQINDIKPLIYTGKDCPK
                     FAVSYEAEVEKYPAVSPKVEISDADDAAMYFSSGTTGFPKAILHTHSSLVSACITENM
                     HHGQTRQDNFLCIPPLYHTGAKMHWFGSFLVGSKAVLLRGVKPKWILKAVSEEKITIV
                     WLLVPWAQDILDCLERGELNLDDYELDQWRLMHIGAQPVPPSLIRRWKTYFPDHDYDT
                     NYGLSESIGPGCVHLGLENIHKVGAIGLAGYGWSTRIVDEKGCDVANGEVGELLVKGP
                     GVMKCYYGDKAATDAVLKDGWLYTGDMAKRDGDGFIYLVDRKKDVIITGGENLYPVQI
                     EDFLRGHDAIKDVAVIGLPDKRVGEIAAAIVELKEGYQCTKEELDAFCLALPRYKRPR
                     AIIFDKVPRNPTGKIEKPKIREKYCKMSVVELQIST"
BASE COUNT          702 a          686 c          456 g          809 t
ORIGIN      
        1 tatccctttt ctctctgaca agattcataa gcagtgcctt acgcaaggaa tcgggactta
       61 agtttcgtac aagccctccg ttcgaagcat aggatattct tccgctctct tccgaaacta
      121 ttaccgctat acagtccgac acctcggtaa tacccaaacc gcctctgtgc ctcatgccca
      181 gttcggtgct cagattgggg ttatcggtaa gaggcaacag acaggctgcg gcaacaattt
      241 tgtccttacg aataataact gcaccgtcgt gtaaaggagt tttaggaaca aatatattct
      301 ccaaaatctc ttttgacaca tcggcattca actgtatccc ggtattgata aactccccta
      361 tttttgtgga tttctcaatg actataaggg caccggtata cctgtccgac atgctctcac
      421 atgcctttac tatctcatcc actaccgtag taatcatcag cgtctcgtcg gaaccggtac
      481 tggagaagaa atgcgtaatc tcactggttc ccagtctctc caagactttg cgtatctccg
      541 gctgaaataa aaccacaacg gacaatgccg caaaggtaat tatattgttg aatataaagg
      601 tcataatctg caagcccaga agattgctta caattgcaac aaacacaata accagcaacc
      661 ctttcagcag gctccatgct cttatttcct ttacaaaaat catcagttta tatacaagga
      721 aggaaacaat cgctatatcg attaatgcgg atataacggc aaaaggactg gacaattcca
      781 aactgaaata tttaatttgt tctacccaat ctatctgatt aaacattgtg cccccacata
      841 ggtcttagta cattaccaca ctaattatat atcattatat aacggtatgc acatatttta
      901 atgcactttt tatatacttt aaatgcaatt aaacggttta tgtactgatc tgcagctcca
      961 ccacactcat cttacagtac ttctcccgta tcttgggctt ttcaatctta ccggtaggat
     1021 ttctgggaac cttgtcaaaa atgatggctc tgggtctttt atacctcggt aatgccagac
     1081 aaaaggcatc caactcttct tttgtacatt gataaccctc tttcagttcc acaatagcgg
     1141 cggcaatttc ccctactctt ttatcgggaa gtcctatgac tgcaacatcc tttatcgcat
     1201 catgacctct taagaaatct tctatctgta cgggataaag attttcaccg cccgtaataa
     1261 tgacatcctt ttttctgtcc acaagatata tgaaaccgtc accgtcccgc ttggccatat
     1321 cacccgtata aagccatccg tctttgagaa cggcatcggt agccgctttg tcgccgtagt
     1381 aacatttcat tactcccggc ccctttacca gcaattcacc cacttcgccg tttgcaacat
     1441 cgcacccttt ttcatccact atcctggttg accatccgta tcccgcaagt cctatcgctc
     1501 cgactttatg tatattctcc agccccagat gcacgcatcc cggtccgatg gattcactca
     1561 gtccgtaatt ggtgtcatag tcatggtcgg gaaaatatgt tttccagcgt ctgataagac
     1621 tgggaggaac aggttgtgcc cctatatgca tcagtctcca ctggtccagc tcatagtcat
     1681 caagattcag ttctcccctt tccagacagt ccaaaatatc ctgagcccac ggtacaagaa
     1741 gccatacaat ggttattttc tcttcactta ccgccttaag tatccacttg ggctttacac
     1801 ccctaagaag aaccgctttg ctgcccacca gaaagctgcc gaaccagtgc attttggctc
     1861 cggtatgata caacggaggt atgcataaaa aattatcctg tctggtttgt ccgtgatgca
     1921 tgttctccgt aatgcatgcc gacaccagac tggaatgagt atgcagtatc gctttaggaa
     1981 atcccgtggt tcccgatgaa aagtacattg ctgcatcatc ggcatcggat atctccacct
     2041 tcggagaaac agccgggtat ttttccacct cagcctcata acttacggca aacttcggac
     2101 agtcctttcc ggtataaatc agcggcttta tatcattaat ctgctctttt atgctctcta
     2161 tcctgcttat gaactcatag ccgaatataa gggcgttgct ttccgacaaa ttcaaacagt
     2221 accttatttc ttcagccgta tacctgtaat tcaaaggcac aaccaaagca ccggttttaa
     2281 gtataccgaa atatataggc agccactcaa tacaattcat cagaagtatg gcaacctttt
     2341 tccctttggt aattccgtta gccatcagaa aattggcaaa tctgttggct ttgtcatcaa
     2401 attctttcca tgttatttct tttctgtaat gttctttggg atttgtttcg ataagttcga
     2461 attccttcca tgtcacttgt cgcttttcct gcaattcggg gtttatctcc actaaactca
     2521 catcatcggg atacagcccg gcgtttttct ccagtatctc cgtaatcggc atatctttcc
     2581 taccttcctg ccttgctaca attattttac tgccaaaatt atatcttctg gacagattct
     2641 tttttctatt ata
//
LOCUS       DAOPWC010000207         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_40599_length_2639_cov_2.815789, whole genome shotgun sequence.
ACCESSION   DAOPWC010000207 DAOPWC010000000
VERSION     DAOPWC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40599_length_2639_cov_2.815789"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKJ65_05085"
     CDS             <1..294
                     /locus_tag="PKJ65_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41242.1"
                     /translation="FVDTILGNTEADTIDIYEALDMFLPGMFAYRSVLAGGIPMDIPN
                     LRIASERDKWRNDTWCTDPAVAKDQLVPSYSKGNPDIPDETYQRIYEKWINGV"
     gene            328..1563
                     /locus_tag="PKJ65_05090"
     CDS             328..1563
                     /locus_tag="PKJ65_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41243.1"
                     /translation="MSKVKIGVFGVRRGSVVIKYCRTQPDAQLVAVCDIWEQGLTDMK
                     KELGEDIAYYTSFDDFINHDMDAVFLANYATEHAPYAIRAFEKGLHVLSEVLPCQTMK
                     EAVELIEAIEKSGKIYAYAENCCFMAAPYEMRKLYRSGQLGELEYGEGEYLHNCESIW
                     PRITRGDKNHWRNNMYAHFYCTHSFGPFIHITGLRPVSVTGFELPFNARMERMGAKSG
                     SAGLAIVTMENGAIIKNINGVGCSKNSLWFSIYGTLGRAESAREDTQYGAYGRIFTNL
                     DRYEGECFERPGTYCPGADQPSPAQKWGHGGGDYYIMHHFVDRISGNMDAETIDIYEA
                     LDMFLPGMFAYRSVLAGGIPMHIPDLRIASERDKWRNDTWCTDPAVAKDRLVPSYSKG
                     NPHIPDETYQRIYEKWLKE"
     gene            1792..>2639
                     /locus_tag="PKJ65_05095"
     CDS             1792..>2639
                     /locus_tag="PKJ65_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41244.1"
                     /translation="MNAESRLKKRKEMLKRGLGKIKSTGYLVFSVLLFVLILVGLLFF
                     YFEFETLAGEYKVRLETMAYALSSGIPNEVFDRYDKNVSYADNKLYKEVTESIKTYVA
                     KNRDIVGISLFVIREGELIPFIQTGDINNHVSKNCIHEVIKSALDNNTKVSYVPAFSD
                     IPEDNAFIAVPVAGGGSGGFLALETSTAVLYEQIKSQHLISVSMFFLIVIFYIIVGKT
                     FMRSSSPHEDKKKMSESVNALKEKEMLFRSIFEQAAPGISINSVDEELDTVPESIKVN
                     DSFKRIT"
BASE COUNT          791 a          469 c          632 g          747 t
ORIGIN      
        1 tttgtcgaca cgatcttggg aaataccgaa gcggacacca ttgatatata tgaggcgttg
       61 gatatgttcc ttcccggaat gtttgcctat cgctctgtat tagccggagg aataccgatg
      121 gacattccaa atcttcggat agcttcagaa agggacaaat ggcgtaatga cacatggtgt
      181 accgatccgg ctgttgctaa agatcagctt gtaccgtcat attccaaggg taatcccgat
      241 atccccgatg aaacatatca gcggatttat gaaaagtgga taaacggtgt ataattcagt
      301 cattatggaa aatataaaga ggtgtatatg agcaaggtga aaattggagt attcggtgtg
      361 agaagaggtt cggttgttat aaagtactgc cggacacaac ccgatgcgca actggtagca
      421 gtctgtgaca tttgggaaca aggtcttacg gatatgaaaa aggagttggg tgaggatatt
      481 gcctactata cctcctttga tgactttata aaccacgata tggatgcggt gtttcttgcc
      541 aactatgcaa cggaacatgc accgtatgca atacgtgctt ttgaaaaggg tctgcatgta
      601 ttgagtgaag tgttgccctg tcagacaatg aaagaggctg ttgaactgat agaagccata
      661 gaaaaaagcg ggaagatata tgcatatgcg gagaactgct gttttatggc tgcaccttac
      721 gagatgcgga aactttatcg ctcgggtcaa ttgggagaat tggaatacgg agaaggagag
      781 tacctgcaca attgcgaatc catatggccg agaattaccc gaggagataa aaaccattgg
      841 cgtaataaca tgtatgctca tttttattgc acacactcat tcggtccttt tattcacata
      901 accggtcttc gcccggtatc ggtaacgggt tttgagttgc ctttcaatgc aagaatggaa
      961 aggatgggtg ctaaatcggg cagcgccggt ttggcaattg taactatgga gaacggagca
     1021 atcattaaaa acattaacgg tgtcggatgt tctaaaaact ctctttggtt cagtatatac
     1081 ggaacactgg gcagagcgga aagtgcaaga gaggatactc agtacggtgc atacggccgt
     1141 attttcacca atctggacag atatgagggg gagtgttttg aaagacccgg tacctattgt
     1201 cccggagcgg atcaaccttc accggctcaa aaatgggggc acggaggcgg cgattattat
     1261 ataatgcacc acttcgttga ccgtatttcg ggaaatatgg atgcggagac tatcgatata
     1321 tatgaagcgt tggatatgtt ccttcccggc atgtttgcct atcgctcggt attggccggc
     1381 ggaataccta tgcatattcc cgatcttcgg atagcttccg agagagacaa atggcgtaat
     1441 gatacatggt gtaccgatcc cgccgttgca aaagaccggc ttgtaccttc atattccaag
     1501 ggaaatcctc atatacccga tgaaacttat cagcggattt atgaaaaatg gctgaaagag
     1561 taacttttta tgacataagg agttgtatta ttattcactt tgtatgataa tattaccagt
     1621 tccgaaaaaa accttgttga aacttctaca gttttgtggt gctgtaaaag tccggagcgg
     1681 gtgaattatt gaaaataatg ccatgattgt gtcagacaac tgcattgtac aaaagattat
     1741 tatgtctttt aattgcaggt aacttgttta aaggaaacat tgggtaacaa catgaatgct
     1801 gaatcgagac tcaagaaaag gaaagagatg ctaaaaaggg ggttaggtaa aataaaaagc
     1861 acagggtacc ttgtattttc agtgttactc ttcgtattaa tcttggtcgg actgctgttt
     1921 ttctatttcg aattcgagac tctggcaggt gagtataagg tcaggcttga gactatggct
     1981 tatgcgcttt cttcaggtat acctaatgag gttttcgaca gatatgacaa gaatgtttcc
     2041 tatgcggata ataagttata taaggaagtg accgaaagta tcaaaacata tgtagcaaaa
     2101 aaccgagata ttgtaggcat ttcgcttttt gtcatacggg agggagaatt aattcccttt
     2161 atacaaacag gtgatattaa taatcatgtg tcgaaaaatt gcatacatga agttattaaa
     2221 tctgcccttg ataataatac aaaagtttcc tatgtacccg ctttttccga tatccccgaa
     2281 gacaatgcct ttattgcggt accggttgcg ggtggcggtt ccgggggttt tcttgcgttg
     2341 gaaacatcta cagctgtact ctatgagcag attaagagtc agcaccttat atcggtatcc
     2401 atgtttttcc tgattgtcat cttttacata attgtgggaa aaacttttat gaggagcagt
     2461 tctcctcatg aggataagaa aaaaatgtcg gaatcggtaa atgctttaaa ggaaaaagaa
     2521 atgcttttca gaagtatttt tgaacaggcc gctccgggaa tatccattaa cagcgtagat
     2581 gaggaacttg ataccgttcc cgagtccata aaggttaatg attcatttaa aagaataac
//
LOCUS       DAOPWC010000208         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_40974_length_2621_cov_2.787997, whole genome shotgun sequence.
ACCESSION   DAOPWC010000208 DAOPWC010000000
VERSION     DAOPWC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40974_length_2621_cov_2.787997"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKJ65_05100"
     CDS             complement(<1..434)
                     /locus_tag="PKJ65_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41245.1"
                     /translation="MKKEKNLLIYILAIIIVFQSIIFILNLNKNGDVEYINNPLEEVI
                     SMNIRRKYYESYNVVDMRCRLLGGKFQCSDYPDFKDAITIYTIDHTDIPYLIETNVSR
                     PYRYWRYVSPDGSWGSKSEVSFHDAQGNKISGRGMANPEAGP"
     gene            complement(785..858)
                     /locus_tag="PKJ65_05105"
     tRNA            complement(785..858)
                     /locus_tag="PKJ65_05105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(822..824),aa:Ile,seq:cat)
     gene            992..1921
                     /locus_tag="PKJ65_05110"
     CDS             992..1921
                     /locus_tag="PKJ65_05110"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNZ41246.1"
                     /translation="MSDNITGRYKERYAATVGFFDGVHKGHLYLLEQLKSVAASRNLK
                     SMAVTFPEHPRQILQSDYRPRLLSSPKEKMQMLEGTGIDLCFPLHFTTALAQMSAESF
                     MSDFLQSRLGVQTLLVGHDHRFGHEPDTVTADYIRIGEKIGMEVVAVDAYLHEGIPIS
                     SSRIRRELEAGRIKEANAMLGYRYTISGTVIHGLHNGRKMGFPTANLGPYCEYMQIPA
                     DGVYSALATVNDETLPAMLNIGYRPTFEGNARTIEAHLLGFDRDIYFQKLTLEFVDFL
                     RPERRFDSPRSLAAQLEIDRSMVREAFRKENIL"
     gene            1949..>2621
                     /locus_tag="PKJ65_05115"
     CDS             1949..>2621
                     /locus_tag="PKJ65_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNZ41247.1"
                     /translation="MLKKRKTLTTLLIWLALQFLCMIPAFLIIFPIAKATDMSMEERG
                     TYMTLITLILAQLLSQLIFRLLKYFRLSETVKPVPKAKTLIMSVAGGISLFYAINIIQ
                     AQFDIPDLLEQQMEGLLSSVWGFIAICILAPISEEVMMRRIILKDMWRKTGKMWGGIL
                     ISSAIFAIIHLNPAQVVFAFPAAIILGWLYAVTGSLSVPILVHMANNSIAFFTSQTDA
                     DDPVLS"
BASE COUNT          752 a          595 c          563 g          711 t
ORIGIN      
        1 tccggtccgg cctcagggtt tgccatacca cgaccagata tcttattacc ctgagcatcg
       61 tgaaatgaca cctctgattt gctcccccat gaaccgtctg gacttacata acgccagtag
      121 cggtatgggc gagatacatt agtttctata agataaggaa tatcggtatg atctatagta
      181 tatattgtta tggcatcttt aaagtcagga tagtctgagc actggaactt acctcctaac
      241 agacggcaac gcatatccac cacattgtat gattcgtaat acttacgtcg aatattcatg
      301 gatattacct cttctaaagg attatttatg tattcaacat ccccattctt attcaaattc
      361 aaaataaaaa taatggattg aaatactata ataatagcta agatgtatat aagcagattc
      421 ttttcctttt tcatgttatt agaataaatt atgtgttatt tttaaaattt ttcgtaacac
      481 tctttgagtg tatacctaaa ttttttcaaa agtattgtat ttttttattt gtcaactatg
      541 tttagtgtta aaaaaactaa ataattaatt ttttaaatcg atgaaataaa gatgttttag
      601 aagttaaata tatttacata gtaactttca gtttaattta taatgacatc acttttttat
      661 atcaattggt tagcgataag atagcagctg aaagagagcc gtatccaaga gccatcagtt
      721 tcgaccaata aggtcaagaa tctaaaatta caggataaaa aaaagtcgcc cgtttggacg
      781 actgtgggcc atctaggact tgaacctagg acctccagat tatgagtctg ttgctctaac
      841 cgactgagct aaaagcccga agaaaaatca tcgaccttga aagggaatca ttttcgcttg
      901 caaaagtagt ccatattaat gaaaatgcaa agttttgagg cgaaaaatga ttaattttgc
      961 gcaaaatcca aaccaataag tatcttgcct gatgagcgat aacattaccg gaagatataa
     1021 ggagagatat gccgccaccg tgggtttctt tgacggtgtg cataagggac acctctatct
     1081 gctggagcaa ctgaagagcg tggctgcaag ccggaacctg aagagtatgg ccgtgacctt
     1141 tccggagcat ccgcgacaga ttctgcagtc agactaccgt ccacgcctgc tctccagtcc
     1201 caaggagaag atgcagatgc ttgaaggcac aggcattgac ctctgcttcc cgctccattt
     1261 cacaaccgca ctggcgcaga tgagcgccga gtcttttatg tccgattttc tgcaaagccg
     1321 gctgggcgtg cagacactcc tggtgggcca tgaccaccgt ttcggacacg aaccggatac
     1381 tgtaacagcc gactatatcc gtatcggaga gaaaatcggc atggaggtag ttgcggtaga
     1441 tgcctatctc catgaaggta ttcccataag cagttcgcgc atacgccggg agctggaggc
     1501 aggccgcata aaggaggcaa atgccatgct gggataccgc tacaccatta gcggtaccgt
     1561 aatacacggt ctgcataatg gacgcaaaat gggattccct accgccaatc tgggtcctta
     1621 ctgcgaatat atgcagatcc ctgccgacgg ggtctattcg gcgctcgcca ccgtcaatga
     1681 cgagaccctg cccgccatgc tgaacatagg ctaccgtccc acttttgagg gcaatgcacg
     1741 caccattgag gcccatctgc tgggatttga ccgcgacata tactttcaga agctcaccct
     1801 tgagtttgtc gattttctcc ggccggaacg ccgctttgac tcaccccgca gtctggccgc
     1861 acagcttgag attgaccgca gcatggtaag ggaggcgttt agaaaagaga atatactcta
     1921 aatcataaac taaaaacaca ggtacacaat gttaaaaaag agaaagacat taaccactct
     1981 gctgatatgg ctggccctgc aattcctctg catgataccg gcttttctca taatatttcc
     2041 tattgccaag gctacagata tgagtatgga agagagggga acttacatga ccctgattac
     2101 ccttatatta gcccaactct tatcgcagct cattttcaga ctcctgaaat acttcagact
     2161 ttccgaaacg gttaagcctg ttccaaaggc gaaaacgctg atcatgtcgg tggcgggcgg
     2221 aataagcctt ttctacgcca ttaatataat tcaggcgcaa ttcgatattc ccgacctgct
     2281 tgagcaacag atggaaggtc tcctgtcgtc ggtctggggt ttcattgcaa tatgtatatt
     2341 ggcccctata tccgaagagg tcatgatgcg ccgcataata ctgaaggata tgtggagaaa
     2401 gaccggaaag atgtggggcg gaatactgat atcgtctgcc atattcgcaa taatacatct
     2461 gaaccccgca caggttgtgt ttgcctttcc cgccgctata atactgggat ggctctacgc
     2521 cgttacagga agtctgtcgg tacccatact cgttcacatg gcaaacaact ccatagcttt
     2581 ttttacatcc caaaccgatg ccgatgaccc tgtgttaagt g
//
LOCUS       DAOPWC010000209         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41090_length_2616_cov_2.867630, whole genome shotgun sequence.
ACCESSION   DAOPWC010000209 DAOPWC010000000
VERSION     DAOPWC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41090_length_2616_cov_2.867630"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKJ65_05120"
     CDS             complement(<1..205)
                     /locus_tag="PKJ65_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41248.1"
                     /translation="MPSQELADVHAGVCQALDSIKVRQMNLEGKDIGITAGSRGIKNI
                     PLILKTVADYIKAAGGNPILIPSM"
     gene            complement(429..1709)
                     /locus_tag="PKJ65_05125"
     CDS             complement(429..1709)
                     /locus_tag="PKJ65_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNZ41249.1"
                     /translation="MDVLLVVVITMTITFSIGLPIALAIGWAALICFFYADLPVAAMV
                     QRMYSSTNTFTLAAIPFFMLAGALMEVGGLSSRLVRFAMSIVGTLNGALAHVQILASC
                     FFAAVSGSAPATAAAIGSALIPEMEREGYPREFSAAVQSVAGTIGTIIPPSIPMVIYA
                     VATGSSIGKIFLAGIIPGIVYALSLMVVVAYQSKKHGYKSNRVFSWEEVWLSFKDAIW
                     ALLVPAIILGGIYSGVFTPTEAGVVAAVYGLIAGTFIYKELNFKKIVKVFADSSINTA
                     MVLLIIAASSAFSWLLSRTGAARELGLWFTGITDNTYIFIGLALLLFFIAGTALETIS
                     AILILTPIIFPIAQQLGIDPIHLGIIVTMTQSHGMATPPLGECINISASIAGCSFDAT
                     VSRIWPFLLAGAIACALVAFVPQLAMFLPNLLYK"
     gene            complement(1712..2230)
                     /locus_tag="PKJ65_05130"
     CDS             complement(1712..2230)
                     /locus_tag="PKJ65_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNZ41250.1"
                     /translation="MSESNVRSKKESGNPIIWVWENIEISICLISMVLMTVFTFSNVV
                     LRYIFGSSIAWAEEVSSFLIIWLTYGGSAYAFRMGAHIGIEALVDKLPPKAKHIVRIA
                     NNILTIAFFVLLAYYSWNYMTQQIANNQLTPVTRVSVAYPISAVPIGSALVIARVIYQ
                     TIQEIKLGVREV"
     gene            complement(2310..>2616)
                     /locus_tag="PKJ65_05135"
     CDS             complement(2310..>2616)
                     /locus_tag="PKJ65_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ41251.1"
                     /translation="SRHFYDPSPLYISKITWDKLTDEQKKIVKDAAIEARDYSRKLAN
                     DRENDSIEEMKAAGLHVTILTPEQIKAFQEATKDVYKEYVDKIGKDFLDRFLKGAGR"
BASE COUNT          784 a          603 c          524 g          705 t
ORIGIN      
        1 ccattgaggg aatgagaatc ggattacccc ctgccgcctt aatatagtcc gcaacagttt
       61 ttaatattaa aggaatgttt ttgattccgc ggcttcccgc cgtgataccg atatcttttc
      121 cctccagatt catttgtcga acctttatac tgtccaaggc ctggcataca ccggcatgga
      181 catctgcaag ctcttgcgag ggcatattct gcttgatcct aatcattcgg ggaaacacaa
      241 tcatcaccct ttccttaatg cattcactcc aatgtttccg ccgatcgcgt cacagacatg
      301 atgatcggat cagaaaggag cattggagtg aatacaatca ctttgcagtt aatttttcaa
      361 agtatttatc atgaagtgta atatttcttc atggactttt ctacttagtt atgaagtccg
      421 cccaaggatc atttatatag caaattaggc aagaacatag ctaattgcgg tacaaatgcc
      481 accagtgcgc aagcaattgc accggcaagc aggaaaggcc aaatacggct caccgttgca
      541 tcgaaggaac atccggcaat acttgccgat atattgatgc actcccctaa aggcggagtt
      601 gccataccgt gagactgagt catagtaacg atgatgccca aatgaatcgg gtcaattccc
      661 aattgttgag caatgggaaa gataatcggt gtcagaatca aaatagccga aatcgtttcc
      721 aatgcagttc cggcaataaa gaacaaaaga agagctaatc caataaaaat gtaggtgtta
      781 tccgttatac cggtaaacca taaacctaat tctcttgccg cacctgtcct tgacagcagc
      841 catgaaaatg cggaagacgc agctataatc agtaatacca tagctgtatt gatggatgaa
      901 tctgcaaata ccttgacaat tttcttaaag ttcagctctt tataaataaa agtacctgca
      961 attaatccgt aaacagcagc tactacccct gcttcagtag gtgtaaagac accgctgtat
     1021 attccgccca atatgatggc ggggaccagc aatgcccaaa tggcatcctt aaaggaaagc
     1081 caaacttctt cccatgagaa gaccctgtta ctcttatatc cgtgcttctt tgactgataa
     1141 gctacaacaa ccatcaatga aagcgcatat acaataccgg gtatgatacc ggcaaggaaa
     1201 atcttcccta tggatgagcc ggtggcaact gcatatatta ccattgggat actgggagga
     1261 atgatggtcc ctatagtacc ggccacggat tgtactgcag cggaaaactc ccttgggtat
     1321 ccttcccttt ccatttcagg aatcaatgct gagcctatgg ctgcagcagt agcgggagcg
     1381 gaacctgata ccgcagcaaa aaagcacgaa gccagaatct gaacatgagc aagggctccg
     1441 ttaagagtcc caactattga catggcaaat cgcactaaac gtgaggaaag gccaccgact
     1501 tccatcaacg cccctgccag cataaaaaat ggaatcgctg ccagcgtaaa agtgttcgtt
     1561 gatgaataca tcctttgtac cattgcagca accggcaggt ctgcgtaaaa aaagcaaatg
     1621 agtgcagccc atccaatagc caaagcaatt ggcaggccga tagaaaaagt aattgtcatg
     1681 gtaataacta caacaagcaa tacatccatt gttacacctc cctcacacct aatttaattt
     1741 cctgaatggt ctgatagatt acccgtgcaa taactaatgc ggagcctatc ggaacagccg
     1801 atatgggata ggcaacagaa actctcgtaa ccggcgtcag ttgattgttt gcaatctgct
     1861 gtgtcatgta attccatgag taataagcaa gtaaaacgaa gaaagcaata gtcagaatat
     1921 tattggcaat tcttacaata tgctttgctt ttggcggtag tttgtcaacc agggcttcaa
     1981 ttccgatatg ggcacccatt cggaacgcat atgctgagcc gccgtatgtc agccatataa
     2041 taagaaatga agatacttct tccgcccaag ctatggaact gccgaatata tatcgcaaaa
     2101 ccacattgga aaatgtgaac actgtcataa gaaccattga tatcagacaa attgagattt
     2161 caatgttttc ccaaacccaa ataataggat tgcctgattc ttttttgctt ctgacattgg
     2221 attcactcat atattacatg cccccttttt tttgattttt tcattcaggt ttgctgtgca
     2281 atatgcacag caaacctgtt gataaaaagt tatcttccgg cgcccttgag gaatctgtca
     2341 agaaaatcct tgccgatttt gtccacatat tctttataaa catccttggt ggcttcctgg
     2401 aaagctttga tctgttccgg agtcagtatt gtcacatgca ggcccgcagc cttcatttcc
     2461 tctatactgt cgttttccct gtcattagca agttttctgg aataatcgcg cgcttcgata
     2521 gctgcatcct taacgatctt cttctgctcg tcagtaagct tatcccaagt tattttactg
     2581 atgtataaag gtgaaggatc ataaaaatgt ctggac
//
LOCUS       DAOPWC010000210         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41296_length_2607_cov_3.270376, whole genome shotgun sequence.
ACCESSION   DAOPWC010000210 DAOPWC010000000
VERSION     DAOPWC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41296_length_2607_cov_3.270376"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKJ65_05140"
     CDS             complement(<1..424)
                     /locus_tag="PKJ65_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41252.1"
                     /translation="MKRIILFLITGVLFASCSNRNAKIEAVHSGVTIVVPQGNLTVRA
                     IADNAFRVTCAPVDMEFPELENLIYMDSSAEAYPVIHEIKRRRIVMRTDPGNISASIN
                     LRNGNITFLDAAGKVLLKEKPDSRIVTPGRTGEFNSLAV"
     gene            complement(571..2418)
                     /locus_tag="PKJ65_05145"
     CDS             complement(571..2418)
                     /locus_tag="PKJ65_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ41253.1"
                     /translation="MAQHKMDDVIPGIDGPLPILDHMPPGAAEGTVGCLHLEKVNSYV
                     IYFNGDPIFYFYADLKFPYPSIFGAESYTLQIYDSKSDTWKDFLYNNEVCTTTGDNFS
                     IDIKYLGNEINFRLKLNGGVMDGYVSNSVLVKQQQRRSRMTSWSSDFGYKEFVGDVFE
                     QPEFHMEAYNAENKKFETVMYPYSYCTYKWYRRNPNSYEMTLIEGADSATYTATADDV
                     GYQLVLLIEGDDEHYSLLFQVESPIIRYPIYSSFEYKGSDGFIINTNYILPEEVLSMI
                     NISCFGEYDTVTQTMPMIDIPINEIVTIKEGQYAFYCDSLTDIIYDFHLPNNQYSLIQ
                     TCTMFGNRMVEELYAGFYSQPMHIQTELDGAPVVRDIELLKKNISGGIEIVNIADSIM
                     HIIGDTIVLDMIIGDYYIRVPGSADNETTYFSNTPLWSDADIVNHPSYTLHWEDSIFV
                     VKLINAIVPTTGSGVVEGEIYEAIGDLNAPARSPFNNHTRNAYTLYLRDANNNLIAKT
                     VSDTDGAYRFSDIPFGEYDVIIDAVGYRMLTQPHHVVLTEETASISGVDYSVTNDGVE
                     ARSIPVGVKSVKTDRSEKDDVVYDLNGRVINAIPEQGIFIRNGKIHFSR"
BASE COUNT          772 a          558 c          484 g          793 t
ORIGIN      
        1 ttacggcaag cgaattgaac tctcccgtgc gtccaggcgt aactatgcgc gaatccggtt
       61 tttccttaag aaggactttt cctgcagcat caaggaaggt tatattaccg ttgcgaagat
      121 tgattgaagc actgatgtta ccgggatcgg ttctcataac aatccttctt ctctttattt
      181 cgtggattac cgggtatgct tccgctgagc tgtccatata gataaggttt tcaagctcag
      241 gaaactccat atccaccggc gcacaagtaa cccggaatgc attatcggca atagccctca
      301 cggtcagatt tccctgagga acaactatgg taacaccgct atgaacggct tcgattttgg
      361 cattccggtt actgcatgaa gcaaacagaa cacctgtaat caaaaagaga ataatccttt
      421 tcatacttca ttattaaaca acttggttag atatatttct ttatacacaa acaacatagc
      481 aacatttctt cgactgtttt cagcgtaaaa acagcgaagc catatgtcat ccacatacaa
      541 aattaaagaa attaattata tggcaaaaaa ttatctgcta aagtgtattt ttccgttcct
      601 aatgaatatt ccctgctccg gaatagcgtt tatcaccctt ccgttcagat cgtaaacaac
      661 atcgtctttc tcacttctat ctgttttgac agacttcaca ccaaccggaa tactccttgc
      721 ttcaacacca tcgttcgtta cgctgtaatc gacaccggat atcgaagcgg tctcttcagt
      781 aaggacaaca tggtgcggtt gcgtcaacat tctgtatcct actgcatcga taattacatc
      841 gtactctccg aacggtatat ccgagaaacg gtaagcgccg tcggtatcgg aaacggtttt
      901 ggcaatcaaa ttattgttgg cgtctctcaa atagagagta taagcgttcc gggtatgatt
      961 gttaaaaggc gagcgtgcag gcgcgttaag gtctcctatt gcttcatata tttcaccttc
     1021 aacaacaccg ctgccggttg tgggcacaat cgcatttata agcttgacaa caaagataga
     1081 gtcctcccaa tgtaaggtat aagagggatg atttacaata tcggcatcgc tccaaagagg
     1141 tgtgttggag aaatatgttg tctcgttatc ggcacttccc ggaactctga tatagtaatc
     1201 gccaattatc atgtcaagta caatggtgtc gcctatgata tgcataatac tgtcggcaat
     1261 gttaacaatc tctattcctc ccgaaatatt ttttttcagc aactcgatat cacgtaccac
     1321 gggagctccg tccagctctg tttgaatatg cattggttga gaatagaaac ctgcatacag
     1381 ttcttctacc atcctgtttc caaacatagt acaggtttga atcaatgaat actgattatt
     1441 tggcaaatga aaatcatata ttatatcagt caaactgtca caataaaagg catattgccc
     1501 ctctttaatg gtaactatct cattgattgg aatatcaatc atcggcatag tttgtgtaac
     1561 ggtgtcgtat tcaccaaagc atgagatatt gatcattgaa aggacctctt ccgggagaat
     1621 ataattggta ttgattataa acccgtcgct tcccttatat tcaaaagagc tgtaaatggg
     1681 gtatctgatt attggcgatt ctacctgaaa taacagactg tagtgttcat catcaccctc
     1741 tattaacaga accaattgat aacccacatc atctgctgtt gctgtataag tggcagaatc
     1801 ggcaccttct ataagtgtca tctcataact gttggggtta cgtctgtacc acttgtatgt
     1861 acaataagag tagggataca tcacagtctc aaatttttta ttttctgcat tgtaagcttc
     1921 catatggaat tccggctgct caaagacatc gcctacaaat tccttgtagc caaaatcact
     1981 gctccaggat gtcattctgg aacggcgctg ctgttgctta accagtacgg aatttgatac
     2041 atatccgtcc attactcctc cgttcagttt aagacgaaaa ttgatctcat tccccaaata
     2101 tttaatatca atgctgaaat tatctcctgt agttgtgcaa acttcattgt tatataagaa
     2161 atctttccat gtatcggact ttgaatcgta tatctgtaaa gtgtagctct ccgctccaaa
     2221 tatcgatgga taggggaatt ttaaatcggc ataaaaatag aatataggat ccccgttaaa
     2281 gtatatgaca tagctgttaa ccttttccag atgtaaacag cccactgttc cctctgcggc
     2341 tccgggaggc atgtgatcca atatgggcag cggaccgtca attccgggaa tcacatcgtc
     2401 cattttgtgc tgggccattg ccatagcgct gaataagaag atcaaacaga aaaaaagctt
     2461 cttaaataaa gtctggtgtt tcattttgtt tggaatttat gaaattaata aaatttatat
     2521 ttctgtaaag ttacttcaaa atatttatca aacaactgtc agctttaaaa taattctctc
     2581 aaaagaacaa aaaagggtta tttgcca
//
LOCUS       DAOPWC010000211         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41478_length_2599_cov_3.470519, whole genome shotgun sequence.
ACCESSION   DAOPWC010000211 DAOPWC010000000
VERSION     DAOPWC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41478_length_2599_cov_3.470519"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PKJ65_05150"
     CDS             complement(<1..1186)
                     /locus_tag="PKJ65_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ41254.1"
                     /translation="MKRMKLISLVLCLVLVCSFIFTACDKKPTETATQRPTETSTQTE
                     VPTETDTTPEEVEKPAAMDITTLYYFGDATENAAEKFAYKNYLNRHYGFTINIHAHAR
                     DMYIETINLRAVSGDLTGIVRLFAGTDGIKWYQEGIVLAIDEYLADNEVWNTIVPEHW
                     KETFSYDGAVYGLAFGGDGTPSWFSRTMRGDWLDAVGMSRPSTIDEFYEVSRAFTYND
                     PDKNGDNDTWGFCSRHVWLMQDIFKAYGCTPNWESALVPVWNPRDDIWEDPVIKPVMA
                     DACTFLRKCYVEGLIHPELFSMSSTNVRNLMSNGHAGSCFYWDHWLIVWENAVKKTVG
                     NHAYMVAVGALSGVVTHKINCWKTEIGSPYVLMANTEQPKEVINWFINWAYGSAEGFF
                     TFR"
     gene            1635..>2599
                     /locus_tag="PKJ65_05155"
     CDS             1635..>2599
                     /locus_tag="PKJ65_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ41255.1"
                     /translation="MVMEYNPKEIESKWQKIWDDLKCFSAKDDLSLPKYYALVEFPYP
                     SGEGLHVGHPRPYTALDIIARKRRLQGYNVLFPMGWDAFGLPTENYAMKHKLHPAAVT
                     KNNIARFKKQLKSIGYSFDWDREISTTDPKYYRWTQWIFLQLFKHGLAYKKNMSINYC
                     TSCKVGLANEEVVDGRCERCGAEVVKKMKNQWMLKITDYADKLIEGLTNLDYLESIKA
                     QQINWIGKSTGAEVYFKLTNILEPLVVYTTRPDTLYGVTYMVISPEHPYLEEYKSLIE
                     NIDEIREYQTESSKKSDFERAEIAKEKTGVEIKGIKAVNPVNNKE"
BASE COUNT          833 a          511 c          510 g          745 t
ORIGIN      
        1 atctgaatgt gaagaagcct tcagcagaac cgtatgccca gttaatgaac cagtttataa
       61 cttccttagg ctgctcagta ttagccatca acacataagg agatcctatt tccgtcttcc
      121 aacaattgat tttgtgagta acaacaccgg aaagagcgcc gacagccacc atgtaagcat
      181 ggtttcctac cgttttcttt accgcatttt cccatactat cagccagtga tcccaataaa
      241 agcaggaacc tgcatgtccg ttggacataa gatttctgac attggtggaa ctcattgaga
      301 acaactccgg atgtatcaat ccttctacat aacactttct taagaatgta catgcatcag
      361 ccataaccgg ctttataacc ggatcttccc agatgtcatc tctcggattc cataccggaa
      421 ccagagcgga ttcccagttg ggtgtacaac cgtatgcttt aaaaatatcc tgcatcaacc
      481 atacatgtct tgaacagaat ccccaagtgt cgttatctcc gtttttgtcg gggtcattgt
      541 aggtaaaggc tcttgatact tcgtagaact catcgatggt ggaaggtctg gacattccta
      601 ctgcatccaa ccagtctcct ctcattgttc ttgaaaacca tgaaggagta ccgtcaccgc
      661 caaatgcaag tccgtatacc gctccgtcat atgagaaagt ttctttccaa tgctcgggaa
      721 ctatggtgtt ccaaacttca ttatcagcaa gatattcatc aattgccaac acaatacctt
      781 cctgatacca cttaatcccg tcggtacctg cgaaaagtct tactataccg gtaagatctc
      841 cggacaccgc tcggagattg atggtttcaa tatacatgtc tctggcatgg gcatgaatat
      901 tgattgtaaa accgtagtgc ctgttgagat agttcttata tgcgaatttc tcagcagcat
      961 tctctgttgc atcaccgaag tagtaaaggg tagtgatatc cattgccgcc ggtttttcta
     1021 cctcttcggg tgtagtatcg gtttccgtag gtacttcagt ctgagtactt gtctctgtcg
     1081 gtctctgtgt tgcagtttca gtcggctttt tatcgcaagc cgtgaaaatg aaagagcata
     1141 ccagtaccaa gcaaagtacc aaactaataa gtttcattct tttcattatt ttcctccctt
     1201 gaataaatat tcaaaactca tatctattat acactttttt actggccagt caagcaaggc
     1261 gcctaacatt ttgttgaata gaaacaaatt aatattgaca aaatattcat aaaatttaac
     1321 aaaacaggtg ttttaatatg tttttaatta aaaatcatga aaatgtaata taaaaaggac
     1381 aagcgcaaag ttagactaat ttccgagaga gctttctgaa ataataagtt tcatgcatta
     1441 aagccaacat ttagttgcaa taaagtctat attatgtaat gtttgagtag cataatcaga
     1501 agaatcttac ttaaaaatgt tagataatcg ttctttataa agagtatttc tgctccgttc
     1561 aacagatata ccttttcttg acaatgtacc gtatttacaa taaaatactg ggaagaatat
     1621 ttgttacgga gagtatcgtt atggaatata atcccaagga aatagagtca aagtggcaga
     1681 agatatggga cgaccttaaa tgtttcagtg cgaaagacga tttatcatta cccaagtatt
     1741 atgctttggt ggagtttccc tatccttcag gtgaaggact gcatgtaggt catcccaggc
     1801 cgtatacggc tttggatata attgccagaa aaagaagact gcaaggatat aatgtgctgt
     1861 ttcccatggg atgggatgca ttcggattac ctacggagaa ttatgccatg aagcataagc
     1921 tgcatcctgc cgctgtaacc aaaaacaata tagcaagatt taaaaaacag ctcaaatcca
     1981 taggatattc ctttgactgg gacagagaaa taagtacaac cgatcccaag tattacagat
     2041 ggacgcagtg gatttttctg caattattca aacacggact tgcatataaa aagaatatga
     2101 gtatcaacta ctgtacttcc tgcaaagtag gtcttgccaa tgaagaagtg gtggacggca
     2161 gatgcgaaag gtgcggagcg gaagttgtaa agaagatgaa gaatcagtgg atgcttaaga
     2221 ttaccgacta tgccgataag cttattgaag gtcttaccaa tctcgattat ctggaaagta
     2281 taaaagcaca gcagattaac tggataggta aatccaccgg agcagaagta tatttcaaat
     2341 tgacaaatat cttagaacct cttgttgtgt ataccacaag accggatact ttatacggag
     2401 tgacatatat ggtaatatct ccggaacatc cgtatttaga ggaatacaaa tcattaattg
     2461 aaaatattga tgaaatccgt gaatatcaga ccgaaagttc caagaagagt gatttcgaaa
     2521 gagcggagat agccaaagaa aagacaggag tggagataaa aggcattaaa gccgtaaatc
     2581 ctgttaacaa taaagagat
//
LOCUS       DAOPWC010000212         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41529_length_2597_cov_3.403226, whole genome shotgun sequence.
ACCESSION   DAOPWC010000212 DAOPWC010000000
VERSION     DAOPWC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41529_length_2597_cov_3.403226"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1691
                     /locus_tag="PKJ65_05160"
     CDS             81..1691
                     /locus_tag="PKJ65_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41256.1"
                     /translation="MEDTLKILIQMQSLDDKIGQYRQLQDELPKQLNSILEKVELATA
                     KLLETETQRSELSKKLRAVEMDIKTHQEQAKKYATQLTDIKTNKEYKALNSEIAYLHS
                     KISDLESAELELMDEDAVFKQQIDEDKAALAAAEQEKKDKEGEDASEEKPVNNTSPLW
                     LKDAKDCTDEEYKAFYREVFRDFKEPLFWIHLKMDYPVNLRGILYFPKLTNEYNTLEG
                     KIQLYCNQVYVADNIKEVIPEYLLLLKGVIDCPDLPLNVSRSFLQNDGYSGKIAGHIS
                     KKVSDKLKQLYKSDAEQYYKYWDDINIFVKYGCLKEEKFYDKVKDIIVYKDIYDKHHT
                     LEQYRKINEEKKHKDVIYYVSDMQKQAQYINIFKEHQQNAVILDNILDSHFLTFIESN
                     NPGTSFMSIDSGIKDVLAEEGATEGFDDIKKFFLELLKDKKVSVEVEALKSSDVSAVI
                     ISDEYESRMKNMQTMYGMEFPSSNTLVLNTQHPVISKLAAMKDERKKSDIAIYIYQLA
                     MLSAGKLEKEDMTDFLKRNNEMLNKSTE"
     gene            1733..>2597
                     /locus_tag="PKJ65_05165"
     CDS             1733..>2597
                     /locus_tag="PKJ65_05165"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNZ41257.1"
                     /translation="MGNIRRIYIERKSGFDAEAKGLLDQCVNLLGIKKLEHIRILNRY
                     DIQGMDDSIYAVAKYGIFAEIQTDTVYEEEFDAKDAAYVFAYEFLPGQYDQRADSTEQ
                     CIQLISHTRPIVKTAKVVCLYGNVKNEDIEAIKKYLINPVECRIISIKKPDNLTEHFE
                     VPQSVDTVTGFITFHSSELKTLKQQYAFAMSDEDLLFVQQYFRSVHRDPTITELKVLD
                     TYWSDHCRHTTFNTVLSDITIEKDKYNETCESTYKRYLEIRKQLYKGKEKDISLMDTA
                     TIGARYLKSKGL"
BASE COUNT          921 a          470 c          578 g          628 t
ORIGIN      
        1 ggggtgcact atatctcctc tggggctatg acgcattcat ataaatcatt ggatatcagt
       61 ttattattta aggagtaagg atggaagaca ctttaaagat tctcatacag atgcaaagcc
      121 tggacgataa gatcggtcaa taccgtcagt tgcaggatga attacccaaa cagctcaatt
      181 ctatcctgga aaaagtggag cttgccaccg ccaaactcct ggaaaccgaa actcagagat
      241 cggaactgag taagaaactc cgcgccgtgg aaatggatat caagacccat caggagcaag
      301 ccaagaagta tgccactcag ctcacggata taaagactaa taaagagtat aaagccctca
      361 atagcgagat agcctatctg cacagcaaga tctccgatct ggaaagcgcc gagctcgaac
      421 tgatggatga ggatgccgtc ttcaaacaac agatcgatga agacaaagct gccctggctg
      481 ctgcagagca ggaaaagaaa gataaagaag gtgaggatgc atcggaagaa aaacccgtta
      541 acaacacttc acctctgtgg ctgaaagatg ccaaagactg tacggatgag gaatataagg
      601 cattttaccg ggaagtcttc cgtgatttta aagagcccct gttctggatt catctgaaga
      661 tggactatcc ggtcaatctt aggggcatac tgtatttccc taaacttacc aatgaataca
      721 atacattgga aggtaaaata caactttatt gcaaccaggt atatgtggcg gacaacataa
      781 aggaagtcat accggagtat ctgttgctgt taaaaggcgt tatagattgt cccgatttgc
      841 cgttaaacgt ttccagaagc tttttacaga atgacggtta ttcgggaaag atagccggcc
      901 atatatccaa aaaggtatcg gataaactga aacagttgta taagagcgat gcggagcaat
      961 actacaagta ctgggatgat atcaacattt ttgtcaagta cggatgctta aaagaagaga
     1021 agttctacga taaggtaaag gatatcatcg tatacaagga tatatacgat aagcatcata
     1081 ctttggagca atataggaag attaatgaag agaaaaagca caaggatgta atatattatg
     1141 tctccgatat gcaaaagcag gcacagtaca tcaatatatt caaagaacat caacaaaacg
     1201 cggttatact ggacaatata ctggattcgc attttcttac atttatagaa tccaataatc
     1261 ccggaacctc ttttatgagt atagacagcg gcattaagga tgtattggcg gaagaaggag
     1321 cgaccgaagg atttgacgat attaaaaagt tcttcttgga gcttctgaag gataaaaagg
     1381 tcagtgtgga agtggaggca ttaaaatctt ccgatgtaag tgcagttata atatccgatg
     1441 aatatgaaag cagaatgaag aacatgcaga caatgtacgg tatggaattc ccttcgagca
     1501 acacccttgt gcttaacaca cagcatccgg ttataagtaa gctggccgct atgaaggatg
     1561 agcggaagaa gagcgatata gccatataca tttatcaact ggcaatgctt tccgcaggta
     1621 aattggaaaa agaagacatg acggattttc ttaaaagaaa taacgaaatg cttaacaaga
     1681 gtacggaata gtgtagtatt atataattaa ataaatgcgg agcgtgcgga ctgtgggaaa
     1741 tataagaaga atatatattg agcggaaaag cggatttgat gcggaagcga aaggtctttt
     1801 ggaccaatgt gtaaacttac tgggaataaa gaaactggag catatccgta ttctcaacag
     1861 atacgatata caggggatgg atgacagtat atatgccgta gctaaatacg gcatttttgc
     1921 cgaaatacag accgatacgg tatacgaaga agaatttgat gcaaaggatg ctgcatatgt
     1981 cttcgcatat gagtttcttc ccggacaata tgatcagaga gccgattcca ccgaacagtg
     2041 tatacagctc atatcccata caagacccat agtaaagacc gctaaggtag tatgcctgta
     2101 cggaaatgtg aaaaatgaag atatagaagc aataaagaag tatcttatca atcccgtaga
     2161 gtgccgtatt ataagtataa aaaaacctga caatcttacc gaacacttcg aggtgccgca
     2221 aagtgtggat accgtaaccg gctttataac tttccattca tccgagttga aaacacttaa
     2281 gcaacaatac gcatttgcca tgagcgatga ggatttactg tttgtacagc aatatttccg
     2341 atcggtacac agagatccca ccattaccga actgaaagta ttggatacct actggtcgga
     2401 tcactgccgg cataccacat tcaataccgt tttgtccgat ataacgatag aaaaagacaa
     2461 atataacgag acatgtgaaa gtacttataa aagatattta gagataagga agcaactgta
     2521 taaaggtaaa gaaaaagaca taagtcttat ggataccgca accataggtg ccagatacct
     2581 taaaagtaaa ggactgt
//
LOCUS       DAOPWC010000213         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41769_length_2588_cov_4.435847, whole genome shotgun sequence.
ACCESSION   DAOPWC010000213 DAOPWC010000000
VERSION     DAOPWC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41769_length_2588_cov_4.435847"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PKJ65_05170"
     CDS             complement(<1..1335)
                     /locus_tag="PKJ65_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ41258.1"
                     /translation="MKKSVRITFAWLLIITLCFSFVSVGAQEDFFDARYYWLYSSAIP
                     SAWKLLADIPTESVTVAVIDTGIESDNPHLKDRIDSRGRNFVLSYASDKVEDKQGHGT
                     AIAGIIAAVPTEENNYYGVCGNVDVKILPLKVYGDISGTLQMQYIIDAIYYAIEMNAD
                     VINISIDIPNYDEGLQIACLAAYEKSIPIIASAGNKSSEYYSYPSSCYGVISVSALSL
                     YDDDYFLSSPFSSYNDQVDLSAHGEDIYTIGLNGTYNFLSGSSFSAAIVAGAAAILKA
                     VNKSLTPLEIETILCASADKLTDKGRNNYYGYGKLNLFSALTLDLDSLIYQSETQQPS
                     EGTSPFSFEYTDRKTTLSCGLSYGLWLDQNNKVMSAGDSSFGKSDVAQWSDIISVAAG
                     DSFSLGLRSDGTLRYTGFSGSYALNVAHWTSLKQVSAGSLHTAAVTTAGYVLT"
     gene            complement(1383..2360)
                     /locus_tag="PKJ65_05175"
     CDS             complement(1383..2360)
                     /locus_tag="PKJ65_05175"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNZ41259.1"
                     /translation="MEKGKFILDFKNRQHRVAVISVVIAVLLIVLSVLLFKLFNKSYN
                     SYEGNSPSNIINNGLVACEDGYLYYTRFDAGSKIFRLDIKSGKEKLVYAANAKYLNVL
                     AGYIYYIDGSNGNIKRITVSGTDELLLKDSICSNLYVTTDWIYYIDNKENNSIYRMSM
                     HDYIPEKLAEESATSVSVHDGFLYFINYLDNNGIYRLDLKTKKVSPVLKQHTIFYTVK
                     DNLIYYTNTNDESYIYSMNLDGSGNKRLEGVKGWFLANSPDDPEFLYFMSPTGSMKKV
                     NLSSLNQMTVTNDECAYINIVENFIVYLNRDDNHYYILDKTTGKSTVLR"
     gene            complement(2394..>2588)
                     /locus_tag="PKJ65_05180"
     CDS             complement(2394..>2588)
                     /locus_tag="PKJ65_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNZ41260.1"
                     /translation="SIDYDAGIYIHAKTGDSIKKGQLLATLYTNRKHILPDAENEYLS
                     SLTFSENLPPPQKLIYQIVK"
BASE COUNT          763 a          528 c          515 g          782 t
ORIGIN      
        1 agtcaaaaca taacctgcgg ttgtaaccgc cgcggtatgg agagaacctg ccgaaacctg
       61 ttttagcgat gtccaatggg ccacattcag agcataggag ccggagaaac cggtatatcg
      121 caatgttccg tcacttctta atcctaaaga aaaggaatct cccgcggcaa cggatattat
      181 atccgaccat tgagcaacat cggattttcc gaaagaagaa tcaccggcac tcataacctt
      241 attattttgg tccaaccata atccgtaaga aagaccgcag gacaatgttg ttttacggtc
      301 ggtatattca aaagaaaaag gagaagttcc ctccgagggc tgctgtgttt ccgactgata
      361 tatcagggag tccaaatcca gagtcaatgc ggagaacaga ttgagcttcc cgtaaccgta
      421 ataattattt cttcccttat cggtaagttt gtccgcactt gcacacaata tggtttctat
      481 ttccagaggg gtaaggcttt tattgacagc tttgagtatt gccgccgcac cggctactat
      541 tgcagcggaa aaagaggaac cgcttaaaaa gttatatgtt ccgtttaatc ctatcgtgta
      601 aatatcttct ccgtgagcac ttaaatccac ctgatcgtta taggaagaaa acggagagga
      661 taaaaagtaa tcatcatcgt acaatgaaag agccgatacc gatattacac cgtagcagga
      721 agagggataa gagtaatatt cactgctctt attgccggca gaggctatta tgggaatgga
      781 tttctcatat gcggccagac aagctatctg cagaccttcg tcatagttgg gaatatctat
      841 acttatatta attacatccg cattcatctc aatcgcataa taaatagcat ctattatgta
      901 ttgcatctgc aatgtgccgg aaatatcgcc gtatactttt aaagggagaa ttttaacatc
      961 cacattaccg cagactccgt aataattgtt ttcctcggta ggaactgctg caatgatgcc
     1021 ggcaattgcc gttccgtgtc cctgcttgtc ttcaactttg tcggaagcat aagaaagcac
     1081 aaaattacgt ccccgcgaat caattctgtc tttcaagtga ggattatccg actcgattcc
     1141 ggtgtctatt accgcaacgg tgaccgattc tgtgggaata tccgcaagca gtttccaggc
     1201 ggaaggtatg gcggaagaat acaaccagta atacctggcg tcaaaaaaat cctcctgtgc
     1261 accgacagaa acaaaggaaa agcataaagt gattattagc agccaagcaa atgtaatcct
     1321 tacagatttc ttcataatat tataataata caatatccgg aataaatcag caatgccgac
     1381 gattatctta aaactgtact tttaccggtt gttttgtcca gtatataata atgattgtca
     1441 tcccggttga gataaacaat gaaattttcc acaatgttta tatatgcaca ttcgtcattg
     1501 gttacggtca tctggttaag tgatgaaaga ttcactttct tcatagatcc ggtaggtgac
     1561 ataaagtaaa gaaactccgg atcatccggt gagtttgcaa gaaaccagcc cttgacacct
     1621 tccagccgct tgttccccga accgtcaaga ttcatgctgt agatatagga ttcatcgttt
     1681 gtatttgtgt aatagataag attgtctttt acggtgtaga agattgtgtg ctgtttaaga
     1741 accggtgaca ctttttttgt tttcaaatcc agacgatata tgccgttatt atccagatag
     1801 tttatgaagt acaggaaccc gtcatgaacc gaaacggagg ttgcgctttc ctcggctaat
     1861 ttttccggaa tatagtcgtg catactcatt ctgtaaatgg aattgttctc tttattgtca
     1921 atatagtata tccagtctgt agttacataa agattggaac atatgctgtc tttcaatagc
     1981 agttcatcgg ttcccgaaac ggttatcctc tttatatttc cgttgctgcc gtcaatatag
     2041 taaatatatc ccgccagcac attcaagtat ttggcatttg cggcatatac gagtttttct
     2101 ttaccgctct ttatatccaa cctgaatatt ttggagccgg catcaaatct ggtgtagtaa
     2161 aggtaaccgt cttcgcatgc tacaagaccg ttgtttatga tattggacgg agagttgcct
     2221 tcataactat tgtatgattt attgaacagt ttaaaaagaa gcactgaaag aacaatcaga
     2281 agaacggcaa taactacgga tattaccgca actctatgct gtctgttttt gaaatccaat
     2341 ataaatttgc ctttttccat gcctaaacaa ctccaatgcc caatattata ctatcatttt
     2401 actatttggt atataagttt ttgtggaggc ggcaagtttt cggaaaaggt caaagagctc
     2461 agatattcat tttccgcatc aggcagtata tgttttctgt tggtatatag tgttgccaga
     2521 agctgacctt tttttatact gtctcctgtt ttagcatgta tatatattcc cgcatcataa
     2581 tcaatgga
//
LOCUS       DAOPWC010000214         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41943_length_2582_cov_2.404828, whole genome shotgun sequence.
ACCESSION   DAOPWC010000214 DAOPWC010000000
VERSION     DAOPWC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41943_length_2582_cov_2.404828"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>2582
                     /locus_tag="PKJ65_05185"
     CDS             17..>2582
                     /locus_tag="PKJ65_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41261.1"
                     /translation="MKIVTVNNIESVPLFASGRAYITQGVSFTEQDYSSGNKVCLISD
                     QLAKSNNIKVGDVLKLSLWNAGYELKTSFLETAWTPKSHGNKNALSDSENYTVAGIYL
                     DDDAWNTQCCTFTPNTVFVPELSINDSMKLQSSLTDLGGDRFSGIPDSRTYILKTGKA
                     SDFEAEMKTAGYGGIFYYYDKNDPSLPYLYALSDNFRITTYMSTAVLAVFIISMAMHA
                     IVINLASTDKKVRLRAVLTVIIISIISIAISAVISLSFYGSVINTTNEYLTTKASTGK
                     ALHTDSSLPLPSASIEQEKLDSVPTQLLMIFAVQTGILLLILCMSSILCFAIKGGKKK
                     TKIPYRLKKGEGIKGGMPAVIWYSITQSLRSNVMGMLIVVLVTLSGIFMFLSVSTWLT
                     AKNSISEAEKAYTTIAVASENEFLKNVNTYTKGIYEYSSLADIYEAAEQSEHVEKVDI
                     RDKCMAYNNGIKTISSGIVDEFTPITAVDYPYDLSVVVGTCVSVEYTVGAERENVFGS
                     FGGADVFLAYTAVLELDRKESPVLSEEYDYSKYIKVNTRQFGTDPNEYFRVGERYILY
                     GSKGYSQYPKTERYRDNPAPFNLETILPEGKYTLINMNREDPDFAFIHFNPKMHEILG
                     TDKDTLVERKGPGKKVYYNINYCYFCAYRDDTNVAYSRLDSSLEEFLDSDKGEKWKRH
                     IEECKTTISSLKMNFTKNLDSIPLFNQGLAYITEGRTFTEQEYSEGAKVCIISAETAR
                     QSGIKIGDKIDFNFWSNGYIVTSNSKQHLWQHASFSKENGFIDEGTFEVIGIYKAENV
                     WSEKEFFFTPNSAFAPKGSIEKPIDLDLPTFIINGKYIEETGEYIPATTGKYQSIPN"
BASE COUNT          845 a          468 c          527 g          742 t
ORIGIN      
        1 aacagcaaaa gaatccgtga agatagttac tgtaaacaat attgaaagtg ttcctctttt
       61 tgcctccggc agagcatata ttacacaagg agtttctttt acagaacaag actattcctc
      121 cggtaataaa gtttgtctca taagtgacca actggcaaag agtaataata ttaaggtagg
      181 tgatgtctta aaattgagtt tatggaatgc cggttatgag ttaaagacaa gttttttgga
      241 aacagcatgg actcctaaat cgcatggcaa caagaatgca ttatcagata gtgaaaacta
      301 tactgttgca ggcatttatt tggatgatga tgcatggaat acccaatgtt gtacttttac
      361 tcccaatacg gtatttgtgc ctgaactatc tataaacgat tctatgaagt tgcagtcttc
      421 actgacagat ttgggcggag acaggttctc cggtataccc gattcgagga catatattct
      481 caaaacaggt aaagcgtctg attttgaggc tgagatgaaa acggcgggat atggcggaat
      541 attttactat tatgacaaaa acgatccttc cttgccttat ctttacgctc tgtcagataa
      601 cttcagaatt actacatata tgtctacggc ggttcttgca gtgtttataa tttctatggc
      661 tatgcacgcc attgttataa atttagcaag cactgataaa aaagtgcgac tgcgtgcagt
      721 tcttacggtt atcatcattt ctattatctc cattgctata agtgcagtta tatctctgtc
      781 cttctatgga agcgttataa acacaacaaa tgaatactta acaacaaaag cctctaccgg
      841 aaaggcatta cataccgatt cctcactccc tcttccgtca gcatcaatcg aacaagaaaa
      901 actggactct gttcctaccc agcttcttat gatatttgct gttcagacgg gaatacttct
      961 tctgattctt tgtatgtctt caatattatg ttttgctata aagggcggaa aaaagaaaac
     1021 aaagatacca tataggttaa aaaaaggtga aggaattaaa ggtggcatgc ctgccgtgat
     1081 atggtacagc atcacacagt cgttaaggtc aaatgttatg ggaatgctta ttgtagtgct
     1141 tgtaactctc tccggtatat ttatgttctt aagtgtgagt acatggctga cagcaaaaaa
     1201 cagcatatcc gaagcggaaa aagcatatac taccattgct gtagcttcgg agaacgaatt
     1261 tttgaaaaat gttaatacct atacgaaagg aatatatgaa tacagctcgc ttgctgacat
     1321 atatgaggct gcggaacaat ccgagcatgt tgaaaaagtt gatatccgtg ataaatgcat
     1381 ggcttacaat aacggtataa agacaatttc ctcaggtatt gttgatgaat tcactcctat
     1441 aactgctgta gactaccctt atgatttgtc tgtagtagta ggaacatgcg tatctgtaga
     1501 atatactgta ggagctgaaa gggaaaatgt tttcggatct tttggaggtg ccgatgtatt
     1561 cttggcatac acggcagtac tggaactgga tcgtaaggaa tcccccgtgc taagtgaaga
     1621 atatgattac agcaagtata taaaggttaa tactcgacag tttggcaccg atccgaacga
     1681 atactttcga gttggagaaa gatacattct atacggatca aagggttatt cgcaatatcc
     1741 caagacggaa agatataggg ataatcctgc cccgtttaat cttgaaacga tactgccgga
     1801 aggcaagtat acacttatta atatgaacag agaagatccc gactttgcgt ttattcattt
     1861 taaccccaaa atgcacgaga ttctggggac tgacaaagac actttagtgg agcgtaaagg
     1921 tcccggaaaa aaggtctact acaatataaa ttattgttat ttctgtgcat acagagacga
     1981 tacaaatgtt gcttatagca gacttgacag ttcactcgaa gagtttctcg attccgacaa
     2041 aggagaaaaa tggaaaaggc atatagagga atgtaaaaca accatcagtt cacttaaaat
     2101 gaatttcacg aaaaatcttg atagtatacc tctgttcaat caaggtcttg cgtacattac
     2161 tgaaggtcga actttcacgg aacaggagta ttccgaagga gcaaaagtat gcatcatcag
     2221 tgcggaaacc gcgagacaaa gcggaataaa gataggagac aaaatagact ttaatttttg
     2281 gtctaacggg tatatcgtaa cttcaaattc caagcaacat ttgtggcagc atgcttcatt
     2341 cagtaaagaa aacggtttca ttgatgaagg caccttcgag gtcataggta tatacaaagc
     2401 agagaatgtt tggagcgaaa aggaattctt ctttactccc aatagtgctt tcgcacctaa
     2461 agggtctatt gaaaagccta tagaccttga tcttcccact ttcataatta acggtaaata
     2521 tattgaagaa acaggtgaat atattcctgc tactacgggt aagtatcaaa gcatccccaa
     2581 ta
//
LOCUS       DAOPWC010000215         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_41956_length_2581_cov_4.342439, whole genome shotgun sequence.
ACCESSION   DAOPWC010000215 DAOPWC010000000
VERSION     DAOPWC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41956_length_2581_cov_4.342439"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKJ65_05190"
     CDS             <1..213
                     /locus_tag="PKJ65_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNZ41262.1"
                     /translation="QRYKGLGEMSSAQLWETTMNPATRTIIQIKMDNPFLADIAVTDL
                     MGDDVQPRKEFIERHAKDVQLNRLDI"
     gene            245..1018
                     /locus_tag="PKJ65_05195"
     CDS             245..1018
                     /locus_tag="PKJ65_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ41263.1"
                     /translation="MGKVIAVANQKGGVGKTTTSINLSASLAFMGKRTLLIDNDPQGN
                     ATSGLGVDKWTVKQSVYDAIINDIDIKDTILATMLDNLFICPANIQLAGAEIELVSLM
                     MRENRLKKAIEPIKNNYDYIIIDCPPSLGLLTLNSLTAADTILVPIQCEYYALEGLKQ
                     LMKTVSLVQKHLNPSLKVEGVVMTMYDSRTNLSFQVVESVRQHFSDRVYRTIIPRNVR
                     LSEAPSHGLPIILYDKDSKGALSYLDLAEEVINCGGENG"
     gene            1011..1859
                     /locus_tag="PKJ65_05200"
     CDS             1011..1859
                     /locus_tag="PKJ65_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ41264.1"
                     /translation="MAKYGLGKGLGALLGDDYTDISVADKENRLIEMDIDKIQRDPGQ
                     PRRDFSKEEMDELAESIREKGIIQPILVRKTGDTYTIVAGERRFRAAKQEKLKTVPVI
                     VKEFTDREVLEISLVENIQRKDLNPIEEAEAYKRLCDEFGATQEEIAKTLGKSRPVVT
                     NKLRLLNLEEKILSFIRRGKLSEGHARLLLSVHEDIREDLAEETVNKELSVRDLEKLI
                     KKTKRTPGKKEQIENDEEFAKVENKLQKKLATKVTITRSIKKGKITIEYYSLEGLNRI
                     IDILSR"
     gene            1881..2375
                     /locus_tag="PKJ65_05205"
     CDS             1881..2375
                     /locus_tag="PKJ65_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HNZ41265.1"
                     /translation="MDYLIYALISLGISLIVSLLIVLVYNENKKKKKKREKEREISID
                     KIKTYEDLLIQLSMIYEKTQENERKIRYLDLQSDKLNKLADISFKNVGLVRYKYSDDA
                     GGNLSFSLALMDDKQNGIVFTNISLIDSNSLYVRRITNGISDVLLMEEEKEAIKQARE
                     SSDI"
     gene            2463..2553
                     /locus_tag="PKJ65_05210"
     tRNA            2463..2553
                     /locus_tag="PKJ65_05210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2498..2500,aa:Ser,seq:tga)
BASE COUNT          963 a          407 c          575 g          636 t
ORIGIN      
        1 cagagatata aaggactggg agagatgagc tctgcacagt tgtgggaaac aacgatgaat
       61 cctgccacca ggacaattat acagataaag atggataacc cgttcctggc ggatattgcg
      121 gtaacagatt taatgggcga tgatgtacaa ccaaggaaag aattcataga aagacatgca
      181 aaagatgtac aactgaacag attagatata taatgtttca cgtgaaacaa atgaaggggt
      241 cgatatggga aaagtaatag ccgttgccaa ccaaaaaggt ggagtaggca agacaactac
      301 atcgataaac ttaagtgcaa gtcttgcatt tatgggaaaa aggacacttc ttattgacaa
      361 tgatccgcaa ggtaatgcta caagcggctt gggagtagac aaatggaccg ttaaacagtc
      421 cgtttatgat gccataatta acgatattga tataaaagat acaattcttg ctacaatgct
      481 ggacaatttg ttcatttgtc ccgccaatat acagcttgca ggagcggaaa tagagcttgt
      541 aagcttgatg atgcgtgaaa accgactaaa aaaagctatt gagcctataa aaaacaacta
      601 cgattatata ataatcgact gtcctccttc tttgggactg cttactttaa actctcttac
      661 ggcagcagac accattttag tacctataca atgcgaatat tatgctcttg aaggcttaaa
      721 acagcttatg aagacggttt ctttggtaca aaagcattta aatccttctt taaaagtaga
      781 gggagtggtc atgactatgt acgattcgag aacaaatcta tcatttcagg tggtggaaag
      841 cgtaaggcaa cacttttccg acagggtata tagaacaata ataccgagaa atgtgcgttt
      901 aagcgaggct ccgagccacg gacttccgat tattctatac gataaagact caaaaggagc
      961 tctgagttat ttggatttgg cagaagaagt aataaattgt ggaggagaaa atggctaaat
     1021 acggtttagg aaaagggttg ggtgcgctgt tgggcgatga ttataccgat atttccgttg
     1081 cagacaaaga aaatcggctg attgaaatgg acattgataa gatacaacgg gatcccggac
     1141 agccgcgcag agacttttcg aaagaagaga tggatgaact tgcggaatct atacgggaaa
     1201 aaggcattat tcaaccgata ttggttagaa aaaccggcga cacatacaca atagtggcag
     1261 gagaaagaag attccgggcc gctaaacagg aaaaactgaa gacagttccg gtaatcgtaa
     1321 aggaatttac cgatagagaa gttttagaga tatctttagt agaaaacata caaagaaaag
     1381 acttaaatcc catagaagaa gctgaagcat ataagagatt atgtgatgaa ttcggagcaa
     1441 ctcaagaaga aatagcaaaa actctcggaa aaagtcgtcc ggttgtaact aataagttaa
     1501 gattacttaa tttagaggaa aaaatactca gttttataag aagaggaaaa ctttccgaag
     1561 gtcacgcaag gctgttgttg tctgtccatg aagacataag agaggatctt gccgaggaaa
     1621 cggtgaacaa agaattaagt gtaagagacc ttgaaaaact cataaaaaag acaaaaagga
     1681 ctcccggaaa gaaagaacaa atagaaaacg atgaagaatt tgcaaaagtt gagaataaac
     1741 ttcaaaagaa gcttgcaaca aaggtaacaa taacgagaag tattaagaaa ggtaaaataa
     1801 cgattgaata ttattcgctt gaaggattaa acagaatcat agacatacta agcagataat
     1861 gtttcacgtg aaacaaaata atggattatt taatatatgc gctaataagt ttgggaatat
     1921 cgctgattgt atcattgctt atagttcttg tttacaacga aaataagaaa aagaagaaaa
     1981 aaagggaaaa ggaacgggaa atctctattg ataaaataaa gacatatgag gaccttttga
     2041 ttcagttaag catgatatat gaaaaaactc aggaaaacga acgaaaaatc agatatctgg
     2101 acctgcagag tgataaatta aataagttgg cagacatatc gtttaagaat gtcggtcttg
     2161 taagatataa atattccgac gatgcgggag gaaatctcag cttttcactt gccttgatgg
     2221 atgataagca gaacggtata gtatttacca atatctcgtt aatagacagc aattccctat
     2281 atgtacgaag aataacaaac ggaatatcgg atgtccttct tatggaagaa gaaaaagaag
     2341 ctataaaaca agcaagagag agttccgata tttaacaata tatgtcaatt gagcagctat
     2401 tgacacattt atttaacttt ggtatattca ttaggcgcgc ttgcgcgtga actttgagac
     2461 ttggagagat ggtcgagttg gtttaaggca ccggtcttga aaaccggcgt aggggcaacc
     2521 ctacctaggg ttcgaatccc tatctctctg ccacacggag aagtacccaa gtaggctcaa
     2581 g
//
LOCUS       DAOPWC010000216         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_42001_length_2579_cov_3.437401, whole genome shotgun sequence.
ACCESSION   DAOPWC010000216 DAOPWC010000000
VERSION     DAOPWC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42001_length_2579_cov_3.437401"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..347)
                     /locus_tag="PKJ65_05215"
     CDS             complement(27..347)
                     /locus_tag="PKJ65_05215"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ41266.1"
                     /translation="MQQVFYDFSIFNILKGKKPLLVCDKIFDTFGIKFPCKVMRFDGF
                     SSNPVYEDICTGVEILKKNNCDFIVAVGGGSTIDTAKAIKYYSGTDYPLMAVPTTSGT
                     AIKG"
     gene            complement(350..1219)
                     /locus_tag="PKJ65_05220"
     CDS             complement(350..1219)
                     /locus_tag="PKJ65_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ41267.1"
                     /translation="MSELNMTEKLPVYIGIKEYMRKTRSGKIKTTAKSKGGIAIRITI
                     FALVALTVYGFLTFDYKDIDMAKAVAGTWNNAKVLFGQPKLTYSTFAGALKELFVTFS
                     LGALSTIFGAIIAFFGALLCAKNIANPIVSGITKSFVAFIRAVPTILWVLIFTVAAGL
                     GSVAAVIGLTFHSCGYLIKAYAESIEEVDAGTIEALKASGASFWQIVFQALLPSSISR
                     MTAWTFLRFEINFTNAIAVGAAAGAGGIGFDLYMAGSHYFDLRELGFITYIVFAAVIL
                     LEILATRIKAKVK"
     gene            complement(1219..2082)
                     /gene="phnE"
                     /locus_tag="PKJ65_05225"
     CDS             complement(1219..2082)
                     /gene="phnE"
                     /locus_tag="PKJ65_05225"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HNZ41268.1"
                     /translation="MLSKNADKKVYNDKTENNAERAPRPEKQTSIFVKRKRSFTLVFL
                     GIIVLFAVSALITEYNVITGFESIPKAVVWMAKQFIPDAKAWSRLSSILTKLAETALV
                     SVAVTVCAAICAFFLSLFGTKTTKAHPLVARIVRIIAAFFRNVPDVVWAMLLLFSFGQ
                     NILTGFFALFFATFGMLTRAFIETIDEVSASCVEALYATGATSLQTVFNGVIPSSMPE
                     IVSWVLYMIETNIRSSTLIGMLTATGIGYLFDLYYKRMDYGSASLVVIAIVILVIAIE
                     IISNRIRKVIM"
     gene            complement(2072..>2579)
                     /locus_tag="PKJ65_05230"
     CDS             complement(2072..>2579)
                     /locus_tag="PKJ65_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41269.1"
                     /translation="HYNLVNRLSVIENVLHGRLGYKSSIVGAVGHYTEEEKERAFHIL
                     AMLGLTEQAYKRCDELSGGQKQRVGIARSLMQEPRVLLCDEPIASLDPSSAKVIMDYL
                     EYINETMKITCIANLHQVDVAMAYAKRIIGITGGQILYDGPPEKLTKAKIYEIYQSDE
                     GELITDAK"
BASE COUNT          740 a          577 c          491 g          771 t
ORIGIN      
        1 ccattaaaat actacagcgg taccgattat cctttaatgg ccgttccgga agtggtagga
       61 acggccatta aaggataatc ggtaccgctg tagtatttta tcgccttggc ggtatctatt
      121 gtacttcctc cacctactgc cactataaaa tcgcagttat tcttctttag tatttctacg
      181 cctgtacata tatcttcata aacggggttg gaactgaacc cgtcaaatcg cataactttg
      241 caaggaaatt ttatgccgaa tgtgtcaaat attttgtcac acaccaacag cggtttttta
      301 ccttttaata tattaaaaat tgaaaaatcg taaaaaacct gttgcatcgt catttcacct
      361 tcgcttttat tctggttgcc agtatttcca gcaaaataac cgccgcaaat actatgtatg
      421 tgatgaatcc cagctcgcga agatcaaaat agtggctgcc tgccatgtat aaatcgaatc
      481 ctattcctcc cgctcccgct gcagcaccta cggcaatggc atttgtgaag ttaatttcaa
      541 aacgcaagaa tgtccatgcc gtcatgcgac ttatagagga gggaagcagt gcctgaaata
      601 caatctgcca gaaacttgct ccgcttgctt ttaatgcttc aatagtgcct gcatccacct
      661 cttcaatgga ttccgcatac gctttaatta aataaccgca actgtgaaat gtcaagccta
      721 tgacagcagc tacgctgccc agtcctgctg caacggtgaa tatgaggacc cacagtattg
      781 taggtaccgc ccttataaaa gcaacaaagc tttttgtgat accggataca ataggattgg
      841 caatattctt tgcacatagc agagctccga aaaaagctat tattgcaccg aagatggtgg
      901 aaagcgcacc cagagaaaat gtcacaaaga gttcttttaa tgctcccgca aatgtactgt
      961 atgtcagttt aggttgaccg aacagtactt tggcattgtt ccaggtgccg gcaacggctt
     1021 tagccatgtc aatatcttta taatcaaatg tcaaaaatcc gtatacggta agtgctacca
     1081 gtgcaaatat ggttattctt atagcaatac cgcctttgga ttttgccgtg gtttttatct
     1141 ttccgctgcg agtctttcgc atatattctt ttataccaat atagaccgga agtttttccg
     1201 tcatattaag ttcactcatt acataataac ctttcttatc ctgttggata ttatctctat
     1261 agcgataacc agtataacta tggctatcac cacaagactt gccgaaccgt aatccattcg
     1321 tttgtagtat aagtcaaaca gatatccgat acctgttgca gtaagcatac ctatcaatgt
     1381 ggaggaacgg atatttgttt caatcatgta taagacccat gaaacaattt ccggcatgga
     1441 tgaagggatc actccgttaa ataccgtttg caaagaagtt gcaccggtgg catataatgc
     1501 ttcaacacag gatgcactca cttcatctat cgtttcaata aatgctctgg tgagcatacc
     1561 gaaggtggca aagaacaggg cgaaaaagcc tgtcagtata ttctgcccga aagagaaaag
     1621 taaaagcatt gcccaaacta catccggaac attcctgaaa aatgccgcaa taatcctgac
     1681 tattctggca accaaaggat gtgctttagt ggtttttgtt ccgaataagc ttaaaaagaa
     1741 tgcacatatg gcggcacata cggtgacagc taccgatacc agggcggttt cggccagttt
     1801 tgtaagtatg gaagacagac gcgaccaggc ttttgcatcg ggaataaact gttttgccat
     1861 ccagacaacg gctttgggaa tggattcaaa accggtaatc acattatatt ccgttataag
     1921 tgccgaaacg gcaaacagaa ctataatacc caaaaatacc aaagtaaacg aacgctttct
     1981 ttttacaaat atcgatgttt gcttctccgg tctgggtgct ctttccgcat tgttttctgt
     2041 tttatcatta tatacttttt tgtccgcatt tttacttagc atctgtaatc aactcgcctt
     2101 cgtcagattg atatatttca taaattttcg ctttagtcag cttttcaggc ggtccgtcgt
     2161 acaaaatttg tccgcctgtg attccgataa ttcttttggc atatgccatt gcaacatcta
     2221 cctgatgcaa attggcaata caggtaattt tcatggtttc attaatatat tccaaataat
     2281 ccataataac cttggctgaa ctggggtcaa gggatgctat gggttcatca cacagaagca
     2341 ctctcggttc ctgcataagg gaacgggcaa tacccactct ttgcttttgc cctccggaca
     2401 attcatcgca acgtttatag gcctgttccg taagtcctaa cattgccaga atatgaaaag
     2461 ccctttcctt ttcttcttca gtataatggc ctacagctcc gactatcgac gatttatatc
     2521 ccaggcgtcc gtgtaaaaca ttttcaatta cgcttaagcg gtttacgagg ttatagtgc
//
LOCUS       DAOPWC010000217         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_42845_length_2545_cov_3.542169, whole genome shotgun sequence.
ACCESSION   DAOPWC010000217 DAOPWC010000000
VERSION     DAOPWC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42845_length_2545_cov_3.542169"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKJ65_05235"
     CDS             <1..482
                     /locus_tag="PKJ65_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ41270.1"
                     /translation="QGICAACGMAQMGKLDKKGYRVYAVLGDGELQEGQVWEAMMYAA
                     HYKLDNLTAFLDHNGLQIDGKITEVMSPEPVVAKFEAFGWSVILIDGHDMNQIDKAIK
                     KAKRTKGKPTMIVAETIKGKGVSYMENKYEWHGKAPNQEQRDIAIAELDAYIESLGV"
     gene            490..1425
                     /locus_tag="PKJ65_05240"
     CDS             490..1425
                     /locus_tag="PKJ65_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNZ41271.1"
                     /translation="MKKATRAAYGEALAEYGSNERIIVLDADLSKSTMTAKFQELYPE
                     RFFNTGIAEANMISSAAGIATCGKIVFASSFAIFAAVRAAEQIRNSVCYPRLNVKIAA
                     THAGISVGEDGATHQAIEDIAVMRALPNLTVIQPCDGRETKQAVKAIIDYDGPVYLRL
                     GRLPVEDIYDNSYEFAWGKGVQLKDGCDVTIIATGLMVGSALEAYEMLKAQGILARVI
                     NIHTIKPIDREIIVKAAKETGAVVTAEEHTIIGGLGSAVCEVLSEEYPVPVKRIGIED
                     KFGKSGKPNELLKLYGLTAEDIAIKAKNVMALKRK"
     gene            1422..2216
                     /locus_tag="PKJ65_05245"
     CDS             1422..2216
                     /locus_tag="PKJ65_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ41272.1"
                     /translation="MIFETHAHLLAEQFDQDRNEVIQRALENGILAVLNVSDDMKSSK
                     KAVMLAQQHERVFGSVGVHPHNGADFRDQDVSILKELTSDEKILAIGEIGLDYYYDFC
                     DRTVQREVFVKQLDLAKQLDLPVIIHNREAHKDTIDILSSYGKDIKGVMHCYSGSAES
                     AKILLNLDMYISFTGVITFKNAKRAVEALEVIPLDRLLLETDCPYMSPEPLRGTRNEP
                     KNLIYIARKMAEVKKISYERLLEQLWENSFRLFKKAEKYKQTLETT"
     gene            2213..>2545
                     /locus_tag="PKJ65_05250"
     CDS             2213..>2545
                     /locus_tag="PKJ65_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNZ41273.1"
                     /translation="MKNTDNIIPLPDYVRAKKALGQNFLTDKTIALKIVEALNLCKED
                     IVIEVGAGTGAITALIAPLCKKLYAVETDIDLIKPLTSVLAKYDNVEIINKDILKTDI
                     NGLIDKKLR"
BASE COUNT          862 a          448 c          621 g          614 t
ORIGIN      
        1 ggcagggaat ctgtgcggca tgcggtatgg cccagatggg aaaactggat aaaaaaggtt
       61 acagagttta tgccgtgttg ggcgacggag aactgcagga aggacaggta tgggaggcta
      121 tgatgtatgc cgctcattac aaactggata atcttacggc ttttctggac cataacggat
      181 tacagataga cggaaaaata accgaagtaa tgtcacccga accggtagta gccaagtttg
      241 aagctttcgg ttggagcgtt attctgatag acggacatga tatgaatcag atagataagg
      301 caattaaaaa agctaaaagg accaaaggta aacctaccat gattgttgcg gaaactatta
      361 aaggtaaggg tgtatcatat atggaaaata aatatgaatg gcacggaaag gctcccaatc
      421 aggaacaaag ggatatagcc attgccgagc tggatgcgta tatagagagt ctgggggtgt
      481 aaattaaaaa tgaaaaaagc tacaagagcg gcttacggtg aagcgttagc ggaatacgga
      541 agcaatgaaa gaataatagt acttgatgcc gatttgtcca aatccaccat gacggccaag
      601 tttcaggaat tgtatcccga gcgattcttc aatacgggaa ttgccgaagc caacatgata
      661 tcctccgcag cgggtatagc cacatgcggc aagatagtat ttgccagttc ttttgccata
      721 ttcgcagcgg taagagctgc cgagcagata agaaactcgg tgtgctatcc caggcttaat
      781 gtcaagatag ctgcaactca tgcgggtata tcggtaggag aggacggtgc aactcatcag
      841 gcaatcgagg atatagcggt aatgagggca ttacccaacc tgacggtaat tcagccgtgt
      901 gacggaagag aaaccaaaca ggcggtaaaa gcaataatag attatgacgg accggtatac
      961 ttacgtctag gaagactgcc ggtggaagat atatatgata actcatacga atttgcctgg
     1021 ggtaagggcg tacaacttaa agacggctgc gatgtaacaa taatagcaac gggacttatg
     1081 gtaggttccg ctctggaagc ttatgagatg ttaaaggctc agggtatttt ggcaagggta
     1141 ataaacatac acaccataaa gcctattgac agggaaataa ttgttaaagc ggcaaaagaa
     1201 acgggtgccg ttgtgactgc cgaagaacat accataatcg gcggactggg ttccgcggta
     1261 tgtgaagttt taagcgaaga gtaccccgtt ccggttaaaa gaataggaat tgaagacaaa
     1321 ttcggaaaat cgggaaaacc taatgaactt ttgaaacttt acggtctgac ggcggaagat
     1381 atagccatta aagcaaagaa tgtaatggcg ctgaaaagaa aatgatattt gaaacacacg
     1441 ctcatcttct tgcagaacag tttgaccaag acagaaatga agttatacaa agagctttag
     1501 agaacggcat attggccgtt ctcaatgttt ccgacgacat gaaatcttcc aagaaagccg
     1561 ttatgctggc acagcaacat gaaagagtat tcggctccgt cggagtacac cctcataacg
     1621 gagcggattt cagggatcaa gatgtcagta tacttaagga acttacttcc gatgagaaaa
     1681 tacttgcaat cggtgagatc ggtctggatt attactatga cttttgcgac cgaacggtac
     1741 aacgggaggt ttttgtaaaa cagctggatt tggcaaaaca actggattta cccgtgataa
     1801 ttcataacag agaagcacat aaagatacaa ttgatatctt atcttcctac ggtaaagata
     1861 taaaaggggt aatgcactgc tattcgggaa gtgccgagag cgcaaaaata cttcttaact
     1921 tagatatgta tatttccttt accggagtta ttacattcaa aaatgctaaa agagccgttg
     1981 aagcattgga ggtcatccct ttggacaggc tgctgctgga gaccgattgt ccgtatatgt
     2041 cacctgaacc tttgagaggg accagaaatg agcctaaaaa tctgatatat attgcaagga
     2101 aaatggccga ggtcaagaag atatcgtatg aaagactttt ggaacaattg tgggaaaaca
     2161 gtttcagact atttaaaaaa gcggaaaaat acaaacagac attggaaaca acatgaagaa
     2221 tacagacaac attatacctc tccctgatta tgtaagagcg aagaaagctt tgggacagaa
     2281 tttcttaacc gacaaaacta tagctttaaa gatagtggaa gcactgaatc tttgtaaaga
     2341 ggatattgta attgaagtgg gtgcgggtac cggtgccata acggcactta ttgctccttt
     2401 atgtaaaaag ctttatgccg tggagaccga tatcgatctt ataaaacccc tcacttccgt
     2461 tttggcaaag tatgataatg tagaaataat aaataaggat attctgaaaa ccgatattaa
     2521 cggtcttatt gataaaaaat taaga
//
LOCUS       DAOPWC010000218         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_42890_length_2543_cov_3.161977, whole genome shotgun sequence.
ACCESSION   DAOPWC010000218 DAOPWC010000000
VERSION     DAOPWC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42890_length_2543_cov_3.161977"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKJ65_05255"
     CDS             <1..797
                     /locus_tag="PKJ65_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41274.1"
                     /translation="MHNIWSQYEIFVRYSDYNYMDEFVNIMNNIMGFSTDSLKDDTVR
                     TTIEYAKIELCKDIGAVAFGTREENPARYIKELERYLETKPLFTPRNQKIERKVIRDQ
                     LAWSIEPDSAYIHSLNAVTDKDTLVNLFFEEICNAPTYEQQCAVALLSVGKAPGEVVL
                     PVMRELLSSGRYSQYEFIMWLGWRSAVQYFFFGPSRDAQIADELYNIYKNKAFLATLR
                     YCNIHPGDETALMILEMFCSTGNVVRNGNYDFGNDAVTDFEMVFNR"
     gene            complement(872..2047)
                     /locus_tag="PKJ65_05260"
     CDS             complement(872..2047)
                     /locus_tag="PKJ65_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ41275.1"
                     /translation="MANIFRCDMGNERDTDRLARYVMRIAVMAVVLLICWYFRNVLVY
                     IIAAFVVSLIGHPIMNLMRKLRIKGRHAPDWLLSVLTIIIIIGLLLLVITQAVPIVTG
                     IIADASLHAWNSGSNMNPLAVINDWLIERFPSLGADFDVFVLLTDKLQELTSFDNVSG
                     FIGSVTSMVVGTVVALFSVIFISFFFIKDENLFSNIVGALVSDKIEESVAKTINEIKQ
                     LLSRYFVGLVIEMFGVALVDFLGLWLIARMDANYALGISVIAGLLNIIPYVGPLIGEI
                     LGVVLAVILKLGLGMGLDVNLWLYAGIVFAIMLFAQWIDNFIYQPVIYSTSIKASPLE
                     IFVVLLIAGTIGGILGMFLAIPSYTVIRVIAIRFFYNYKPIKRLIPDLQKEDELLNS"
     gene            2262..>2543
                     /locus_tag="PKJ65_05265"
     CDS             2262..>2543
                     /locus_tag="PKJ65_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HNZ41276.1"
                     /translation="MAKDHDNIVSTEISQDRFTLIENGVPTDIFISENEKKGVLIAAE
                     NLRADFERVCSTKAQLLTEAPKGNCIIVGTIDSPIMKTLAKNKKITSVDL"
BASE COUNT          826 a          565 c          501 g          651 t
ORIGIN      
        1 ttatgcataa tatctggagc caatatgaaa tattcgtccg atacagcgac tataactaca
       61 tggatgaatt tgtcaatatc atgaataaca taatgggatt cagcaccgat agtctgaaag
      121 atgacacggt gcgtacgacc atcgaatacg ctaaaataga gctgtgtaag gatattggag
      181 cagtagcatt cgggaccagg gaagagaacc cggcccgcta tattaaagag ttggagagat
      241 accttgaaac aaagcctctt ttcactcccc gcaaccaaaa aatcgaaagg aaagtcattc
      301 gggatcagtt agcctggagc atcgagcctg attcggcata tatacattcg cttaatgctg
      361 taaccgataa ggacacactt gtaaacctct tttttgagga aatttgcaac gctcccacct
      421 atgagcagca gtgcgctgta gcactgcttt cagtgggcaa agctccgggt gaagttgtct
      481 tgcctgtgat gagggaactt ctgtcgtccg gtcgatactc gcaatatgaa ttcataatgt
      541 ggctgggatg gcgcagtgca gtacagtatt tcttcttcgg tccgtcccgt gatgcccaaa
      601 tagctgacga actatataac atctataaaa ataaggcgtt ccttgcaaca ctgcgttact
      661 gcaatatcca tcccggcgac gaaaccgccc ttatgatcct ggagatgttc tgctcaaccg
      721 gcaatgttgt ccgaaacggc aattacgatt tcgggaacga tgccgtgaca gacttcgaaa
      781 tggtattcaa caggtaagtc agccaagtcc agactatata aaatagttgc ttttgggtgg
      841 tttttcaggc tgataatcag ttccctctac cctacgaatt cagaagttcg tcctccttct
      901 gcaggtccgg tataagtcgc ttgataggtt tataattata aaagaaccga atagctataa
      961 cacgtattac cgtatatgaa ggaatcgcca agaacatgcc gagtatgcct cctatcgtac
     1021 cggctatcag taataccaca aatatttcaa gcggactggc tttaatgctt gtggaatata
     1081 ttaccggctg ataaatgaag ttgtctatcc attgtgcaaa caacattatg gcaaatacta
     1141 tgcccgcata cagccacaga tttacatcca gacccatacc caaaccaagt ttaagtatta
     1201 ccgccaatac cacacctaaa atttcaccga taagcggccc cacatatggt attatattaa
     1261 gcagaccggc tattacagat atacccagag catagttggc atccatacgg gcaatgagcc
     1321 aaaggcctaa gaaatcgacc aaagccacac cgaacatctc aatcacaaga ccaacgaaat
     1381 aacgtgaaag aagttgtttg atctcgttta tggtcttagc caccgactct tcaattttgt
     1441 ccgaaaccaa agccccgaca atattactga aaaggttctc gtccttgata aaaaagaacg
     1501 atataaatat gactgagaat agcgctacca cagtgccaac caccattgaa gttaccgacc
     1561 cgataaaacc ggagacattg tcaaaagatg taagttcctg aagtttatcg gtaagcagca
     1621 caaaaacatc aaaatcagcg cccagtgaag ggaaacgttc aatcagccaa tcgtttatca
     1681 cagcaagcgg gttcatatta cttcccgaat tccaggcatg caatgatgca tcggctatga
     1741 ttcctgtcac aataggtacg gcctgagtaa tgaccaacaa cagtaaacct ataataatta
     1801 ttattgttaa gaccgataag agccagtcag gcgcatgcct gcctttaata cgcaatttgc
     1861 gcataaggtt cataatcgga tgtcctatca gcgacaccac aaatgccgct attatataaa
     1921 ccagcacatt gcgaaaatac cagcatatca aaaggacaac tgccattaca gcaatcctca
     1981 ttacatatct ggccaaccgg tcggtatcac gctcattacc catatcgcat ctaaaaatgt
     2041 ttgccaaata tacagaataa atcagaataa agagttctct gccctgcaat acatgtcaaa
     2101 agaaaaatct tacgaactgt tttttgacag atgttttcat tttgttttgt atctttgtgt
     2161 tacataggat taccgcaaac attgtatttt cagaaaataa ataattaagc atgaaaaagg
     2221 gattttgttt actaacctgt attttgacaa tcagcgttgt catggctaag gatcatgaca
     2281 atatagtttc aaccgaaatc agtcaggacc gctttacttt aatagaaaat ggcgttccga
     2341 ccgatatttt catatcagaa aatgagaaaa agggagtact catagctgca gaaaacctcc
     2401 gggcagactt tgaaagagta tgcagcacaa aagctcagtt attaactgaa gctccaaaag
     2461 ggaattgtat cattgtgggt actattgaca gcccaatcat gaaaacactg gccaagaaca
     2521 agaaaatcac gtcggttgac ctg
//
LOCUS       DAOPWC010000219         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43179_length_2531_cov_3.026656, whole genome shotgun sequence.
ACCESSION   DAOPWC010000219 DAOPWC010000000
VERSION     DAOPWC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43179_length_2531_cov_3.026656"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..379
                     /locus_tag="PKJ65_05270"
     CDS             20..379
                     /locus_tag="PKJ65_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ41277.1"
                     /translation="MIRQKKLDVLIGAMCTIKGDINTKGSVTVEGVIEGDITADGDIV
                     FAKSAKAKGQFTGANIILSGEAEGILKAKKYIKMTSTANFIGDTYALSLISDEGAKFT
                     GKCYVVENEKEDKTRKE"
     gene            444..>2531
                     /locus_tag="PKJ65_05275"
     CDS             444..>2531
                     /locus_tag="PKJ65_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ41278.1"
                     /translation="MNEDELLDSYEEEQEESANLEYVEYTPSFISTLLSTFWAALKIV
                     IAVVMIAAFLIGGLGLGIITAWISTSKVLTEEDLAITTGLTTFIYDIDGNVIETLTGS
                     DNINREIITSKQLPVVLEHAIIAIEDERFPTHSGFDFIRIGGSILKLIENRGDIAQGG
                     STITQQVYKNVTGRFEQTFERKIQEIYNAILLEMKYDKKQILTMYANLVNMGNGCYGY
                     QSAAKMYFNKTLNQINLAEAAVLAAIPNSPYTYNPYGTEENIRKLMYRREDVLDKMLE
                     IGYIDQKEYDDAMAYEIVFAEKVDYVSSSITSYFVDYVITEAIDLLAEKNSISKVQAE
                     RIIYNNGLHIYTTLQPKVQDALDSVFTDNSYFAEYKEDGSIININAIRYGEKPQGAMV
                     IIDQNTGGIAGLYGGNGEKTGSRTYNRATGMNRQPGSTFKPIAVYAPALDLKLITAAT
                     VIDDAPCYLDVKNPDLIYPTNYDSADYQGLTSIRNAIKASVNVVAAKVWAYYLGRDNS
                     VEYLERVGIDRSEHIYDDSTVSASIGGLAVGVSPLEMASAFSTFANKGIHMNAHCITR
                     IANNSGETVLRWANTYTVAYSEQTAAIMTDMLEEVVKGVSTTYWFNGTAYGSVYINEG
                     KIAVAGKTGTTSDYLDKWFCGYTPYYTAAVWYGYDNRVSPVSLVKEEYGRALKIWNTV
                     MNLVHKDLPAKQFT"
BASE COUNT          817 a          510 c          607 g          597 t
ORIGIN      
        1 aggcaaatag gagtataaaa tgattagaca gaaaaaactt gatgtcctta taggtgccat
       61 gtgtacgata aaaggagata taaacacaaa aggttcggta acggttgaag gggttattga
      121 aggagatata accgccgacg gtgatattgt ttttgccaaa tcggccaaag caaaaggaca
      181 gtttaccgga gccaatataa tattgagcgg cgaagcggag ggaatattga aagccaagaa
      241 atatattaaa atgacctcca ccgccaattt cataggagac acatatgctt taagcctcat
      301 aagtgatgag ggagccaaat ttaccggcaa gtgctatgtt gttgaaaacg aaaaagaaga
      361 caaaacaaga aaagaatgat gcgggcagat ttgcagtctg ccgtttttcg gcataagtgc
      421 gcttatgtcg gtgaggtaat gtaatgaatg aagatgaact gttagattca tatgaagaag
      481 aacaagagga atctgccaat ctggagtatg tggaatatac tccttctttc atcagcacat
      541 tattaagcac tttctgggcg gcattgaaga tagttattgc ggttgtcatg atagcggcat
      601 tccttatagg gggtctggga ctgggaataa tcaccgcatg gatttccact tccaaagttc
      661 tgaccgaaga agatcttgcc atcaccaccg ggcttacaac ttttatatat gatattgacg
      721 gaaatgtcat tgagacgctg acgggaagtg ataatatcaa cagagagatt ataacttcca
      781 aacagcttcc ggttgtattg gagcatgcga ttatagctat tgaagacgaa cgatttccca
      841 cgcactcggg tttcgacttt atccggatag gcggttctat actgaaactc atagaaaaca
      901 gaggtgacat agctcagggc ggaagtacaa taactcagca ggtgtacaag aatgttacgg
      961 gacgtttcga gcagacattc gagcggaaga tacaggagat atacaatgcc atactgctgg
     1021 aaatgaagta tgacaagaag cagattttaa caatgtacgc aaaccttgtt aacatgggta
     1081 acggatgtta cggttaccag tcagcggcaa aaatgtactt taacaagact cttaatcaga
     1141 taaatcttgc cgaagccgcc gtacttgcgg caatacccaa ttctccctac acatacaacc
     1201 cgtacggtac cgaagaaaat atacgaaaac tgatgtaccg tcgcgaggac gtactggata
     1261 agatgctgga aatagggtat atagaccaaa aagagtatga tgatgcaatg gcatacgaga
     1321 ttgtgtttgc cgagaaggtg gattatgtat cgtcatccat cacatcatat tttgtggact
     1381 atgtaataac cgaagccata gaccttcttg cggagaaaaa ctccatatcc aaggtgcagg
     1441 ccgaaagaat aatatacaat aacggactgc atatatatac cactttacag ccgaaggtcc
     1501 aggatgcact ggacagtgta tttaccgaca actcctattt cgccgaatac aaagaagacg
     1561 gctcgattat aaacatcaat gccataagat acggcgaaaa accccaggga gccatggtca
     1621 taatagacca aaataccgga ggaatagccg gtctttacgg aggaaacgga gagaaaaccg
     1681 gaagcaggac atacaacaga gcgaccggta tgaacagaca gcccggttcc acttttaaac
     1741 ctattgccgt atatgctccc gctctggacc tcaaactcat aaccgcggct acggtaatag
     1801 acgacgctcc gtgttatctg gatgttaaga atcccgacct catatacccc accaactacg
     1861 attcagccga ttatcagggc ctgacgtcca taagaaacgc cattaaggca tcggttaacg
     1921 ttgtagcggc aaaagtatgg gcatattatc tgggaagaga caattccgtg gaataccttg
     1981 agagggtagg tatcgacaga agcgaacaca tatatgacga ttccaccgtt tcggcatcca
     2041 taggaggttt ggcggtaggt gtcagtcctc tggagatggc aagcgcattc agcacatttg
     2101 ccaacaaagg catacatatg aatgctcatt gcataaccag aatagccaac aactccggtg
     2161 aaaccgttct tcgttgggca aatacctata ccgtggctta ctcggaacag acggcagcta
     2221 ttatgaccga tatgctggaa gaggttgtaa agggtgtttc cacaacatac tggtttaacg
     2281 gaacggcata cggttcggta tatataaacg agggtaagat agcggttgcg gggaaaaccg
     2341 gaaccacttc cgactatctg gataagtggt tttgcggcta tactccttac tatacggctg
     2401 cggtctggta cggctacgac aacagagtgt cgcccgtttc tttggtaaaa gaagaatacg
     2461 gaagagcact gaagatatgg aatacggtaa tgaatcttgt gcataaagac ctgcctgcca
     2521 agcagtttac a
//
LOCUS       DAOPWC010000220         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43316_length_2525_cov_4.039676, whole genome shotgun sequence.
ACCESSION   DAOPWC010000220 DAOPWC010000000
VERSION     DAOPWC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43316_length_2525_cov_4.039676"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PKJ65_05280"
     CDS             complement(<1..1219)
                     /locus_tag="PKJ65_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ41279.1"
                     /translation="MGTVSIPVAQVKKAPALLGEPDIMKMVQLLPGIHSGGDGFAGVY
                     IRGGSYDQNDMTMGGITIYNAEHLKGFVSAFNPDVIEEIVVHKGSFPARYGGHISSVI
                     EAKMKDGNFESFHGAGSLGMLSAKLFAEGPIIKEQTSFIVSARISYFNLFTRPILEKI
                     YDKTEALSQFVNMSYFDLNAKITHKLSKKSKISALFYCGGDRLNLDGEMSQMESQQER
                     QGKIETYRKSNQEDINNNWGNILAGVHWNRIYGEKTFLNTGLYFSRFRYELNKNSAIK
                     EESVPLNNPTDSFYQHTNSSIKFLSGIEDYNLNSDVTSLLTANNTLSAGMKLSIKQFS
                     PIVKTHRDITTIIASSQTVDVSDINTGNPSKLINGAIYVEDKWRVNDMFSMNMGLRLN
                     IYGVKDRIYVYPEP"
     gene            complement(1606..2313)
                     /locus_tag="PKJ65_05285"
     CDS             complement(1606..2313)
                     /locus_tag="PKJ65_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41280.1"
                     /translation="MSDYFTIIYTPLRQEITAYIRDEFSSWVIYESTMDVLNNYNVIL
                     IDSASRTYNCHSYAWNISEGGATCWIDYGGINLSKYWTNDLYSSATQGNHYGYTRVYY
                     SGDHSALSSYGTSYYTSKWGEGPLVRHSPTTVPASYTTNRTYYYAPAIIGPAYITLGN
                     TYIYRIFPHIADYANATYHWNFYDEEKGGYIVSSAADSVAIFFNQGGTFQISCSIENS
                     QDTIVYSAFFEPLREYL"
BASE COUNT          708 a          491 c          493 g          833 t
ORIGIN      
        1 gtggttcagg atagacgtat atcctatcct ttacgccgta tatgttaagg cgtaatccca
       61 tattcatact aaacatgtca tttacccgcc atttatcttc aacatatatt gccccgttaa
      121 tgagttttga tgggtttcct gtgtttatgt cagagacatc aaccgtttgt gaggaagcta
      181 taatggttgt tatgtcccgg tgtgtcttga ctatgggtga gaactgtttt atagataatt
      241 tcattccagc ggatagtgtg ttattggctg tcaacaaaga tgttacatcg gagttcaaat
      301 tatagtcttc aatacctgat aaaaacttta tgctggagtt cgtatgctgg tagaaactgt
      361 ctgtagggtt atttagagga acagattcct ccttaatggc tgaattctta ttaagttcat
      421 aacggaaacg actgaaatac agtcctgtat taaggaatgt tttttctccg taaatgcgat
      481 tccagtgcac acctgccagg atattgcccc agttattatt aatgtcttcc tggttacttt
      541 ttctataggt ctctattttt ccttgtcgtt cttgttggct ttccatttga ctcatttctc
      601 catccagatt taggcgatcg ccaccgcaat agaatagtgc ggaaatctta ctttttttag
      661 agagtttatg tgttattttg gcgttaaggt caaaataaga catattgaca aactgtgaca
      721 aagcttctgt tttgtcatat atcttttcca gaatgggtct tgtaaataaa ttgaaatacg
      781 atattcttgc cgagacaatg aacgatgttt gctctttaat gataggtccc tcggcaaata
      841 gtttagcaga taacatgccc agggagcctg ctccatggaa agattcaaaa ttaccgtctt
      901 tcatcttggc ttcaattacc gatgaaatgt gccctccgta ccgggcggga aaacttccct
      961 tgtggacgac gatttcttca atgacgtcgg gattaaaagc agatacaaac cctttaagat
     1021 gttcagcatt gtatatggtt attcccccca ttgtcatatc gttttggtcg tatgagccgc
     1081 ccctgatata caccccggcg aaaccatccc ccccggaatg gatacccggt aataattgaa
     1141 ccattttcat tatatcgggt tctccaagta gtgccggagc tttttttacc tgggccacag
     1201 gaatagatac tgtccccatc tgtgtactgt gagctcctaa atcagactgt cgggcatata
     1261 tagtgatttc gtccagatca ggatttggct gtagtcttac gttcaggata gtgtcgcagg
     1321 taattgtgag ttgtacttct tgcgttatgt atcctacgta gctgaaatct agcagtacgc
     1381 caccttttgg taattttatg gtgtaagtcc catcgctatg tgtgtatgtg tttcgtagtc
     1441 ctacatcgct tataattgaa gccccgttta ggcactcacc tgttaatgag tcgtatacga
     1501 ctcctctcac aacaaatttg ttttgtccgt aaacactagc cggtatcagc aatattaaca
     1561 atattaacaa tttgcaaaga acaaagaaaa ggaatgccct catttttaca agtattcacg
     1621 taacggttca aaaaaggctg aataaacaat tgtgtcttga gaattttcaa tactacaact
     1681 aatttggaat gtacctcctt gattaaaaaa gattgctaca gaatctgcag cagatgatac
     1741 aatatatcct cctttttctt catcataaaa attccaatga taagtagcat tagcataatc
     1801 ggctatgtgt ggaaatattc tatagatata agtattcccc aaagtgatgt atgccggtcc
     1861 gattattgcg ggcgcgtaat agtacgttct gttggtagtg tacgaagccg ggacagtcgt
     1921 ggggctatgt ctcactaatg gaccctctcc ccattttgaa gtataatatg atgtgccata
     1981 tgatgaaagt gccgaatggt caccgctgta atatactctg gtatatccat aatgattacc
     2041 ctgggttgca gagctatata aatcatttgt ccaatatttg gataaattga tacctccata
     2101 atcaatccaa caggttgccc ctccttccga tatgttccat gcataactgt ggcagttgta
     2161 ggtgcgtgaa gcactgtcaa taaggattac attgtagttg ttaagtacat ccatagtgct
     2221 ttcataaatt acccaggaag agaattcatc tcgtatatat gcagtaattt cttgtcttaa
     2281 aggcgtatat atgatagtaa aatagtcact cattgctttc attctctttg actttgacat
     2341 atcaagtaaa acgtaacctt gataaaccaa tgggtttttt cttttctcag atgctttttc
     2401 agacaggtaa aaatataaag agtccttctg tatatcattc agttgagaga taaaagtatc
     2461 atttgtaagt acaatagttc gttaaaaaaa taaaggttac ccgacggctc gaacgccgta
     2521 gaata
//
LOCUS       DAOPWC010000221         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43519_length_2516_cov_4.586753, whole genome shotgun sequence.
ACCESSION   DAOPWC010000221 DAOPWC010000000
VERSION     DAOPWC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43519_length_2516_cov_4.586753"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="PKJ65_05290"
     CDS             complement(<1..1689)
                     /locus_tag="PKJ65_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41281.1"
                     /translation="MENKKKTGVTIIIIVSVVIVAAALLFIFRDSLFGKTEHFNVTTS
                     NSDIVFKRSDAQEPLNMPYRYSKVLMDKRLAFKEEIVSINISSVRYEMSDKGLSLYNY
                     NDVLKNPDSSNVEEVIGSIKYCKGITALSGIISDKKDSKITIYEGYSADLLAENLQHY
                     IIIPSTLSSHINEELPDNEKVLFLINPGTSGLAYFTVIGEYTTKNESDTLYLSYSGLS
                     TVVLGAQKDVPDHIDCMEINVNKQADLTNFSYFLSEYFADMNMLNQYEKRINRFNEPY
                     SYMFVNTVDLKPIDLSGEKSSEKNIITISRIDGKDNLEMSHVYADALINDYSRYSQYI
                     TDLNISTGRKGVNPSDYPAFTKVPSYSFSITLPGTYGREITDYLKKSGKNWPWFHQAV
                     TGVSEIERMKKGCEITFYTNYTGKDLAALRNEDYRKEKELEGDIKGYAIVPVAMHEAT
                     RNNRSIDYHIVTMSESDLSINFLEAMRVGFKIIGYYELPEETKDKVEKDEKEEKIIAV
                     YDKEGNKIEMRESVPDGYDTIFLTYTGYNDKYKKAAFKNEYIEAITIETRSDTDI"
     gene            complement(1771..>2516)
                     /locus_tag="PKJ65_05295"
     CDS             complement(1771..>2516)
                     /locus_tag="PKJ65_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41282.1"
                     /translation="EDYGNRIVIKRIDGKGSLRMSYRYVDKILSDKSYFSGDIEKFLI
                     SSVRIRLDGENFRWGFPLGVLAKTENTEEDRNILLAIQYCKGITDIDIIANEKKNCEV
                     TFYEGFNAATMQDKMRDCVIIPSSITKFINSELAPQDRIMSISLVEGSSVFPGRFTII
                     GEYTARDKYDTIYVSYSGIARILTSAESDVSEFVDSLEIKLFEGQDGIQLMEYLDRYF
                     ADANALEKYEGKTNRFGDPYIFQYEHYPK"
BASE COUNT          686 a          545 c          396 g          889 t
ORIGIN      
        1 aatatcggta tcgcttcttg tttctatagt tattgcttct atgtactcgt tcttgaatgc
       61 cgccttctta tacttatcat tatatcccgt atatgtcaga aatatcgtgt catacccgtc
      121 aggtacagac tcacgcatct ctatcttgtt accttccttg tcatacacag ctataatttt
      181 ttcctctttt tcatcttttt ctaccttatc ttttgtctct tccggcaatt cgtaatatcc
      241 gattatcttg aatccgactc gcattgcctc aagaaaatta atgcttaaat ccgactcact
      301 cattgtaaca atatggtagt ctatactgcg attattgcga gtagcctcat gcattgctac
      361 aggtactatg gcatatcctt ttatatcccc ttccaattct ttttctttcc tatagtcttc
      421 atttcttaat gctgcaagat ccttacccgt atagttggta taaaatgtta tttcacagcc
      481 ttttttcatt ctttctattt cacttacacc ggtaacagct tgatgaaacc acggccagtt
      541 cttgccgctt tttttaaggt aatcagttat ttctctgccg taagttccgg gaagtgttat
      601 cgaaaagcta taagacggta cctttgtaaa tgcagggtaa tcactcggat tcactccttt
      661 tctgcccgta cttatattta aatccgttat atactgtgaa tatcgggagt agtcatttat
      721 aagggcgtcg gcatatacat gcgacatttc cagattgtct tttccgtcaa tccgggatat
      781 tgttattata ttcttctcgg agcttttctc tcccgaaaga tcaataggct ttaaatccac
      841 cgtgtttaca aacatatacg aatacggctc attaaacctg tttatccttt tttcatactg
      901 attaagcata ttcatatctg cgaaatattc gcttaagaag tatgagaaat ttgtaaggtc
      961 ggcctgctta ttcacattaa tctccataca atcaatatga tccggaacgt ctttttgagc
     1021 tccgagaacc accgtactta atcccgaata ggacagatac aaagtatcac tctcattttt
     1081 tgttgtgtat tctcctatta ccgtgaaata tgccaagccg cttgtgccgg ggtttattaa
     1141 aaacaacacc ttctcgttat caggcaattc ttcattgata tgactactca atgtagaggg
     1201 aatgataata tagtgctgca agttttccgc caataaatca gcggaatatc cttcataaat
     1261 ggttatttta ctgtcttttt tgtcagatat tataccgctt aatgcggtaa tacctttgca
     1321 gtacttaatt gaacctataa cctcttcgac attgctgctg tccggatttt tcaaaacatc
     1381 attgtagtta tacaaactta agcccttgtc gctcatctca tacctgacgg aagatatgtt
     1441 gatactgact atttcttctt taaatgccaa ccttttgtcc attagaactt ttgaatatct
     1501 gtacggcata ttaagaggct cctgagcatc tgacctttta aaaacaatgt cgctgttaga
     1561 agtcgtaaca ttgaaatgct ctgttttacc gaaaagactg tcacggaata tgaacagtaa
     1621 agctgccgca acaatcacaa ccgatactat gatgattata gtaacacctg tttttttctt
     1681 gttttccatc tgttaacctc cgttcgtata tttaaatata gatgtttatg caaaccgtgc
     1741 taatcactaa ctaacggtgc tatatataaa ctatttcgga taatgctcgt actgaaaaat
     1801 atacggatcg ccaaacctgt tggttttacc ttcatatttc tcaagagcgt ttgcatcggc
     1861 aaaatatctg tctaaatact ccatcagttg aataccgtct tgcccttcaa acaatttaat
     1921 ttccaatgaa tccacaaatt ccgatacatc gctttctgct gatgtaagaa tacgagctat
     1981 gccggaatat gatacataga tagtgtcata tttatccctt gcagtgtact ctcctattat
     2041 tgtaaaccgg ccgggaaata cggatgagcc ttcaacaagg cttatactca ttattctgtc
     2101 ctgaggagca agttcggaat ttataaattt ggttatggag gacggtataa tcacgcagtc
     2161 tctcatttta tcctgcatcg tagcggcatt aaagccttca taaaatgtaa cttcacagtt
     2221 tttcttttca ttggctatta tatcaatatc cgttatgcct ttacagtact gaatggccag
     2281 taagatattt ctatcttcct cagtgttttc cgttttcgct agaacgccca acggaaatcc
     2341 ccaacgaaag ttttctccgt ccagtctgat tcttacggaa gaaataagaa acttttcaat
     2401 atctcctgaa aaatacgatt tgtccgagag aattttgtct acatacctat atgacattct
     2461 cagagaacct tttccgtcta ttctcttaat gactatgcgg ttgccataat cttctt
//
LOCUS       DAOPWC010000222         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43525_length_2516_cov_3.795205, whole genome shotgun sequence.
ACCESSION   DAOPWC010000222 DAOPWC010000000
VERSION     DAOPWC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43525_length_2516_cov_3.795205"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..2073
                     /gene="gpmI"
                     /locus_tag="PKJ65_05300"
     CDS             433..2073
                     /gene="gpmI"
                     /locus_tag="PKJ65_05300"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ41283.1"
                     /translation="MLKKSSEFKEIQGPLLLIVMDGVGLSDKKEGNAVAAANAPTLRM
                     LMDKYPHTTIKAHGVAVGLPGDEDMGNSEVGHNALGSGQIYSQGAKLVFEAIEKKTIF
                     SGKTWLKLKDNCIKHNSTIHFIGLFSDGNVHSNIAHLKAMLEQAKAEGLKKARMHILL
                     DGRDVGETSALEYVEPFEEYLDTLRCKSFDVEIASGGGRMNITMDRYEADWDMVRKGW
                     EIHVLGKGRTFSSAKEAIETYRAETDVVDQFLPGFVITKDNKPVGTIEDNDSVIFYNF
                     RGDRAIEISRAFEEKNFDKFDRVRVPNVIYAGMLEYDGDLHIPKNYLVSPPDIKNTLG
                     ENLSKKGIKQFAISETQKFGHVTYFWNGNNSAKFEGETYVEIPSDKIPFEQRPWMQCA
                     LITDKLLEALKSGEYKFLRMNFPNGDMVGHTGSFDAAVIGVESVDLCLARMLPVVDRL
                     NGVAIITADHGNAEEMYETKKGIISIDRKTGKPVPKTSHTLNKVPFIIYDNRKDKGYA
                     LKEGDFGLANVAATVVNLLGYVAPETWEESMIEEVYNG"
     gene            2066..>2516
                     /locus_tag="PKJ65_05305"
     CDS             2066..>2516
                     /locus_tag="PKJ65_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ41284.1"
                     /translation="MDNFYELASKRQSCRNYSDRPVEKEKLMQCIKSAAIAPSACNGQ
                     PWHYTLITESETVTKLVRHLQSAGANRFTSKTPAFAVITEKPTNAMASMGERLKDQDF
                     SSMDIGISVAHFVLQAEALGLSTCIIGWFNENGIKELLNIKKSDRIRL"
BASE COUNT          850 a          452 c          566 g          648 t
ORIGIN      
        1 tttaaataat ctgtcacgga ataaaaacaa cagtgaagcc gcaataacta taattacagc
       61 tgcgataatc aaaatagcaa atcttttttt cctgttttcc attctgtcag acctccgatt
      121 taaatacaat aaaattgcat atatactgta tcttcaagac ttaactatcc aaacaatact
      181 ctgtttcttt ttcttcatct atatatattt tttattaata ctgctgctac acacggttca
      241 ttctaacata aaacagagaa ctgttaaata tgctacggaa attgttcata attgttcaca
      301 attggagata tatttattaa taaaacattg tatattcctc ctatattata tccaacagcc
      361 ctaattgatt ggcatattgc ggtatgatat aattttaaag aggtacggaa gtactacgga
      421 aagggataga atatgcttaa aaaaagctct gaattcaaag aaatacaagg accgcttctt
      481 cttatcgtaa tggacggagt ggggttatcg gataaaaaag aaggaaatgc cgtagcggct
      541 gcaaatgctc ctacattgag gatgttgatg gataaatatc cgcatacgac cataaaggct
      601 cacggtgttg cggtaggatt accgggtgat gaggatatgg gcaactcgga agtaggtcat
      661 aacgcattgg gttccggaca gatttacagt cagggagcaa aactggtttt tgaagccata
      721 gaaaagaaaa ccattttcag cgggaaaacc tggcttaaac ttaaagacaa ttgcataaag
      781 cataattcta caatacattt tatagggttg ttttccgacg gcaatgttca ttccaatata
      841 gctcatctta aagcaatgtt ggaacaggca aaagccgaag gacttaaaaa agcgcgtatg
      901 catatattat tggacggaag agatgtcgga gaaacatcgg ccctggaata tgtggagcct
      961 tttgaagagt atcttgatac tttgcgctgt aaaagttttg acgttgaaat tgcaagcggc
     1021 ggcggcagaa tgaatattac catggacaga tatgaagccg actgggatat ggtaagaaaa
     1081 ggctgggaaa tccatgtatt gggaaaagga cgcacatttt cctccgccaa ggaagctata
     1141 gagacataca gggcggaaac cgatgtggtg gatcagtttc ttccgggatt tgtaataaca
     1201 aaagacaata aaccggtggg aacaatagaa gataacgaca gtgttatatt ctacaacttc
     1261 cgcggagaca gggctattga aataagcaga gcatttgaag aaaagaactt tgacaaattc
     1321 gacagagtaa gagtaccgaa tgtcatttat gcgggtatgc tggaatatga cggagacctt
     1381 catataccca agaactattt ggtcagccct ccggatataa aaaacacatt gggagaaaat
     1441 ctctccaaaa aaggaattaa acagttcgca atatccgaaa cacagaaatt cggacatgtc
     1501 acatatttct ggaacggaaa caattccgcc aaatttgaag gtgagacata tgttgaaata
     1561 ccttcggata agataccgtt tgagcaacgt ccctggatgc aatgcgccct tataaccgat
     1621 aagcttctgg aagcgttgaa aagcggagaa tataagttcc tgagaatgaa tttccccaac
     1681 ggggatatgg taggtcacac gggcagtttc gatgcggctg taataggtgt ggaatcggta
     1741 gacctttgtc tggcaagaat gttgccggtt gtggaccgat tgaacggagt agccataata
     1801 acggcagacc acggaaacgc cgaggagatg tacgagacaa agaaaggcat catatcaata
     1861 gacagaaaaa cgggaaaacc ggtgcccaaa accagtcata ctcttaacaa agttccgttc
     1921 attatctatg acaacagaaa agacaagggt tatgcactga aagaaggcga tttcggattg
     1981 gccaatgtgg cggctacggt tgtcaatctt ttaggatatg tggcccccga aacctgggaa
     2041 gaaagtatga ttgaggaggt ttataatgga taatttttac gaattggcat caaaaagaca
     2101 gagttgcaga aactattcgg acagaccggt tgaaaaagaa aaacttatgc agtgcataaa
     2161 gtctgcggcc atagcacctt cggcatgtaa cggtcagcca tggcattata cccttattac
     2221 cgagagtgaa acagtaacta aactggtaag gcatttacag tccgccggcg ccaacagatt
     2281 tacatccaaa acacccgctt ttgcggttat taccgaaaaa cctaccaatg caatggcatc
     2341 tatgggagag cgattaaagg atcaggattt ttcctcaatg gatataggga tatccgtggc
     2401 acattttgta ttgcaggctg aagctttggg attatccacc tgtataatcg gttggtttaa
     2461 tgaaaacggc attaaagaac tgctgaatat taaaaagtcc gacaggataa gattgg
//
LOCUS       DAOPWC010000223         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43903_length_2501_cov_3.765331, whole genome shotgun sequence.
ACCESSION   DAOPWC010000223 DAOPWC010000000
VERSION     DAOPWC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43903_length_2501_cov_3.765331"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PKJ65_05310"
     CDS             complement(<1..1763)
                     /locus_tag="PKJ65_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNZ41285.1"
                     /translation="MLNLINVEKPARYTGGEFNEIVKDHDKMQSSYAFCFPDIYEVGM
                     SHLGMRILYHVINQRKTTVCERVFAPWTDMEQLMRANKVPLFSLETKKPLKEFDIVGF
                     TLQYEMSYTNVLNMLNLADIPIRRSMRTDQHPIIMAGGPCAANPVTLEDVIDIFLIGE
                     GEEIINQVLDLYEDGKSTGQSKEDFLREAVGIEGVYVPKYHDFDKKVVKRIIKDLDSV
                     SFPTDSIVPYIGIVHDRASLELFRGCIRGCRFCQAGYIYRPVREKKASTLIEQTKKIL
                     SDTGYNEIGMVSLSTSDYTDLKTLCDGMLEHTEKRMVNLSVPSMRIDNFSMELMNRIQ
                     NVRKSGLTFAPEAGSQRLRDVINKNITEEEILTSAKIAKSGGYDNIKLYFMLGLPTET
                     DEDVIAIANLAGKILDIFGRKSRINITVSTSFFIPKPFTPFQWCPQITVEEMIRRQRL
                     LKSHIKEKKIKYNWHDFNTSVVESAISRGDRQVGQAIIKAWEKGCKFDSWNELFDMEK
                     WEQAFKECSLDIYGCASKQIALDEELPWDFIDIGIKKSFLKNEYQKALREETTENCKE
                     QCNNCGIAKYRCGVCFEDNKI"
     gene            complement(1770..2048)
                     /locus_tag="PKJ65_05315"
     CDS             complement(1770..2048)
                     /locus_tag="PKJ65_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNZ41286.1"
                     /translation="MDNERDNIIVMEDDEGNPIEMTVLEMLEYDDRTYVLMQSADESE
                     EDAYIFEYEEDKDDAILTPVEDEQLLDELFELFVEKMDSDFDDEEEEE"
     gene            complement(2093..>2501)
                     /gene="ruvX"
                     /locus_tag="PKJ65_05320"
     CDS             complement(2093..>2501)
                     /gene="ruvX"
                     /locus_tag="PKJ65_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ41287.1"
                     /translation="DYGDKRIGVAKSDPLGFLAHGVTTIEWNRDINKPLDVLEEIVDE
                     HKAGRIVVGLPLNMDDSEGEKAIKVRAFASMLEERCKIPVILFDERLSTVEAHDIMHM
                     QGKKTGKDKKRIDMVAACVILQCYLDEMNKTKK"
BASE COUNT          631 a          624 c          391 g          855 t
ORIGIN      
        1 taaatcttat tatcttcaaa acatactccg catctgtatt ttgcaatccc gcagttatta
       61 cactgctctt tgcaattttc ggttgtttct tccctcaatg ccttttgata ttcgttttta
      121 aggaaactct tttttatacc tatgtcaata aagtcccacg gcaactcctc atctaacgca
      181 atctgtttgg atgcacaccc gtaaatatcc aaagagcatt ccttaaatgc ctgttcccat
      241 ttttccatat cgaataactc gttccaggaa tcgaatttgc atcctttttc ccatgctttt
      301 ataatggctt gccctacttg tctgtctccc ctggatatag ccgattccac aaccgaagtg
      361 ttgaaatcgt gccagttgta ctttattttc ttctccttta tatgactttt cagcagtctc
      421 tgccttctta tcatttcttc cacggtaatc tgaggacacc actgaaaagg agtgaagggt
      481 ttcggtatga agaaggaagt ggataccgtt atgttaatcc tgctctttct tccgaatata
      541 tctaaaattt tccctgccag attggcaatc gctatcacat cctcgtccgt ttcggttggc
      601 aatcccagca taaaatacaa ctttatattg tcataaccgc cggattttgc tatttttgcg
      661 gaagtcaata tctcctcttc ggtaatattc ttgtttataa catccctcag cctctgactt
      721 cccgcttccg gtgcaaaagt cagcccggat tttcgtacat tctgtattct gttcatcagt
      781 tccatggaaa aattatctat tctcatggag ggaacggata aattaaccat tcttttctcg
      841 gtgtgctcca gcattccgtc acataaagtc ttcaggtcgg tataatccga tgtgctcagc
      901 gaaaccatcc ctatttcatt atagccggtg tcggaaagaa tcttctttgt ctgctcaatc
      961 agcgtagagg ctttcttttc tcttaccggt cggtatatgt aaccggcctg acagaacctg
     1021 caacctctta tgcatcctct gaacaattct aaagaagctc tgtcatgcac aataccgata
     1081 tacggaacta tggagtcggt aggaaaagaa acagagtcca gatcctttat tattcttttt
     1141 accacctttt tatcgaaatc atgatatttc ggtacatata ccccttctat tccgaccgct
     1201 tctctcaaaa agtcctcttt tgattgccct gtacttttcc cgtcttcata aagatccagt
     1261 acttgattaa taatctcctc accctcaccg ataagaaaga tatctatgac atcctccaaa
     1321 gtgacgggat ttgcggcaca aggtcctccc gccataatta tcgggtgctg atctgttctc
     1381 atacttcgtc ttataggtat atcggccaga ttcagcatgt tcagtacatt ggtatagctc
     1441 atctcatact gtaaggtaaa accgactata tcaaactctt tcaaaggctt tttggtttct
     1501 aaggaaaata acggtacttt atttgcacgc ataagctgct ccatatcggt ccacggagca
     1561 aaaacccttt cgcatacggt agttttcctt tgatttataa catggtacag gatacgcatt
     1621 cccagatgac tcatccctac ttcatatata tcgggaaaac agaatgcata gcttgattgc
     1681 attttatcat ggtcttttac tatctcatta aactccccgc ctgtatatct ggcgggtttt
     1741 tccacattta tgagatttaa cataaaccgt tattcctctt cttcctcgtc atcaaagtcc
     1801 gaatccatct tttccacaaa aagctcgaaa agctcatcca gcaactgctc gtcttcaacc
     1861 ggtgtcagta ttgcatcatc cttgtcttct tcatattcaa atatgtatgc atcctcttcc
     1921 gattcatcgg ctgactgcat caaaacatag gttctatcat catattccag catctccaga
     1981 accgtcatct ctataggatt gccttcatca tcttccatca caataatatt gtctctttcg
     2041 ttatccatta tttttgctcc tttacgcttt tatatacaaa tccgtaatct tattattttt
     2101 ttgttttatt catttcatca agatagcatt gcagtattac acaggctgct accatatcta
     2161 ttcttttttt gtcttttccc gtcttctttc cctgcatatg cataatgtca tgagcctcta
     2221 cggtggaaag cctttcgtca aacagtataa cgggtatttt gcaacgctct tccagcatgg
     2281 atgcaaaggc gcgcacttta atggcttttt caccttccga atcatccata ttgagaggaa
     2341 gccccaccac tatgcgacct gctttgtgct catcaacaat ctcctctaat acatccagag
     2401 gtttatttat gtctctattc cattctattg ttgtaacacc gtgagccaga aaacccaaag
     2461 ggtcactctt ggcaacccct attcttttgt cgccgtaatc a
//
LOCUS       DAOPWC010000224         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43918_length_2501_cov_2.365904, whole genome shotgun sequence.
ACCESSION   DAOPWC010000224 DAOPWC010000000
VERSION     DAOPWC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43918_length_2501_cov_2.365904"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKJ65_05325"
     CDS             <1..500
                     /locus_tag="PKJ65_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41288.1"
                     /translation="LYQRGIKTVNSKSGKFYRYAPDALSRMIGASEGYPHFIQQIGAS
                     TFDINENEVINLDTVNRAIFDTHGAIDLIGDRYYRTLYYDSIKEDAYREILKIMAENL
                     SKVTTRKQLLEKFKGSKNTLDNGLSALRNRGIILPVIGKRGEYRLQWVGFGLWIKFFT
                     HCRNQ"
     gene            1014..1618
                     /locus_tag="PKJ65_05330"
                     /pseudo
     CDS             1014..1618
                     /locus_tag="PKJ65_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1630..1724
                     /locus_tag="PKJ65_05335"
     tRNA            1630..1724
                     /locus_tag="PKJ65_05335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1665..1667,aa:Ser,seq:gct)
     gene            complement(1786..>2501)
                     /gene="budA"
                     /locus_tag="PKJ65_05340"
     CDS             complement(1786..>2501)
                     /gene="budA"
                     /locus_tag="PKJ65_05340"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNZ41289.1"
                     /translation="FFLMLTIFSITLSLPAGNRDTLFQVSTFKALSEGIYEGEITFNE
                     LRRHGDFGMGTLLGLDGEVIAVNGRFYKVGFNGKAVPVPGSALSPFAMVTFFSSDKEA
                     VIEKTESLKELGQQLDALLPSKNIFYAIKIEGTFAYIKTRSVEKQKPPYPRLDEVTAK
                     QSVFEFENVRGTIAGFRCPEYIGQTNISGYHFHFITDDRKAGGHLLGCRMEKVRASID
                     YTENFLMRLPRNQSFLNLP"
BASE COUNT          756 a          469 c          578 g          698 t
ORIGIN      
        1 agctttatca acgtggaatt aaaactgtca attcgaaatc agggaaattt tataggtatg
       61 ctccagatgc tctgtcacga atgataggag cttctgaggg gtatccacat tttatacagc
      121 agattggggc aagtacattt gatattaatg aaaatgaggt aatcaatttg gatacagtaa
      181 atcgagcaat ctttgacact cacggtgcta ttgacctaat tggcgaccgt tattacagaa
      241 cattatacta tgattcaatt aaagaagatg cttatagaga aatattgaag attatggcag
      301 aaaacctatc aaaagttacg acacgcaaac aattgcttga aaaatttaaa gggagcaaga
      361 acactcttga taatggtctt agtgcattaa gaaatcgagg tataatcctt cctgtaatag
      421 gaaaaagggg tgagtacagg ttgcaatggg taggattcgg tctttggata aaattcttca
      481 cgcattgcag gaatcaataa aatagttaat atttcgttgg aaattatccc atgttttaga
      541 ttttgttttt cgaaaatttc attgaaatta taaagcttgt taccatactt atcagtacag
      601 tcttcgcccg ctgagataac cttttgtatt gccgctttcc atagcagtaa gcacagaaat
      661 cctcgcaatt caaaaacttg aagttataat tgttatccca ttttttattt ctgcatactt
      721 attccaagac aaacaagaaa tcgtggaaga gagatgcctt gagcataaat aacctctgta
      781 aaacattcaa agggaaatat ctctgcgata ttaacaacat tcatatcgaa tattataaga
      841 aaaatcgatt ggctgatggt gttacacacg ctactataaa tagagaactt gcctgcttaa
      901 ggagcatatt caataaggcg atagagtgga aggtattaac gcaaaatcca ccgaaaatac
      961 tcttgtataa agaaaataat cagcgagtta ggtatttgac tgaagaagaa gccatgttgc
     1021 ttataaatct tgctccggaa cctttaaaat caataattgt tgttgccctt aatacaggta
     1081 tgcggagagg agaaatactg aatttaaagt gggaagatat cgatatcagg gagaaggttc
     1141 tcactttaca ggaaaccaaa gggggtggca agagacatat cccggctaat aatactgtga
     1201 gagaaatatt ttcaagaata aagagtggtg cttctcatga acatgtcttt acaaatagac
     1261 agggaggcgc attaacttat gactacatag cgcaccactt cactaaaatc ggaaagaggg
     1321 ctggtataga gaacttccgg ttccgtgatt taaggcatat gttcgcttca tggttatgga
     1381 ggggattgat ttgaaatcgg tgcaggagtt gcttggacat aaagatttca gaatgacatt
     1441 aagatacgcg catctctctc ctgaacataa gagagcagta gacattcctg ataaaagtgg
     1501 tataatgtta gggatggatt ctcaaaatca gaaaaaagat gctcatgatt caacaagtag
     1561 taacatttta gtaacattag agaatccgga cagcaagaaa aatgatgtaa gtccttgata
     1621 gtcaagcatg gaggggtagc atagctggcc taatgcggcg gattgctaat ccgttgtacg
     1681 gtcaaaaggc cgtaccgtgg gttcgaatcc caccccctcc gccatttagg tatattccgg
     1741 gcacatcctt aacacaatat tccgggcacg gtatattccg ggaaatcagg gcaggttgag
     1801 gaatgactgg ttacggggca gcctcatgag gaaattctct gtatagtcta tagaagccct
     1861 gactttttcc atcctgcacc cgagaaggtg tcctcccgct ttcctgtcat ccgttatgaa
     1921 gtggaagtgg tagccggaaa tgtttgtctg tccgatatac tccgggcacc tgaagcctgc
     1981 aattgtgccc ctgacattct cgaattcgaa taccgactgt tttgccgtta cctcgtccag
     2041 ccgggggtag ggcggtttct gtttttcgac gcttcttgtc tttatgtagg caaatgttcc
     2101 ttcgattttt atggcgtaga atatgttttt ggatggcagg agggcgtcga gctgctgccc
     2161 gagttccttt aagctttctg tcttttcgat gaccgcttcc ttgtcggagc tgaaaaaggt
     2221 taccatggca aagggtgaga gggcggatcc aggaacgggg acggccttgc cgttaaagcc
     2281 taccttgtaa aatcttccgt tgacggctat tacttccccg tcgagcccga ggagggtgcc
     2341 cataccgaag tccccgtgtc tcctgagctc gttgaaggtt atctctccct catagattcc
     2401 ttccgagagg gccttgaagg tggatacctg gaagagggta tcccggttgc cggccggaag
     2461 gcttaaagta atggaaaaaa tcgtcagcat gagaaagaag g
//
LOCUS       DAOPWC010000225         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_43959_length_2499_cov_2.916530, whole genome shotgun sequence.
ACCESSION   DAOPWC010000225 DAOPWC010000000
VERSION     DAOPWC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43959_length_2499_cov_2.916530"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKJ65_05345"
     CDS             complement(<1..285)
                     /locus_tag="PKJ65_05345"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583229.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ41290.1"
                     /translation="MAELIFNLSKEGKCAFSLPESRVPSYEIDKKYTRDDDVLLPEVS
                     EVDVIRHFTNISRNNYGVDQGFYPLGSCTMKYNPKINEDVAGLQGFTSLHP"
     gene            complement(278..1582)
                     /gene="gcvPA"
                     /locus_tag="PKJ65_05350"
     CDS             complement(278..1582)
                     /gene="gcvPA"
                     /locus_tag="PKJ65_05350"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNZ41291.1"
                     /translation="MRYIPVTDRDRQEMLKRCKADSVEHLFSCIPEQVRLKRPLNLPP
                     AKSEMELMKYMQSLAAQNSNTDNYACFLGAGAYDHYIPSVISHLISRQEFLTAYTPYQ
                     PEISQGTLTAIFEYQTMICELTGMDVANASMYDGASALAEACVISSGALRKRSDIIIV
                     EDMHPEYRETVETYLKFRDIKYTSMTVKQLLNADLTNVAGVCVQYPSFYGDIYDMQSI
                     GDYVKQSGSLFIVCANPVALALLKSPKDFGADFVVGEGQPLGMPLGYGGPYLGFFACV
                     KEHMRKMPGRIVGKTTDVDGKTAYVLTLQAREQHIRREKATSNICSNQALCALTATIY
                     LSVTGKKGFKQIAQRCVDNAHYVYEGLLKTGKFEKTSDMPFFHEFTVKPKVNIAALNR
                     YLYGHGFIGGLETDRGWLIAVTEKRTKEEMDEFIRRAGEFNG"
     gene            complement(1582..1950)
                     /gene="gcvH"
                     /locus_tag="PKJ65_05355"
     CDS             complement(1582..1950)
                     /gene="gcvH"
                     /locus_tag="PKJ65_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNZ41292.1"
                     /translation="MRFTRDHEWIKKEGKYAYIGITDYAQQQLGDIVYVELAEEDLVL
                     QQGDVLCAIESVKTAAEVYMPVSGTVIEANAELEDAPEKINEDPYGTYLAKIIIDDES
                     ELNDLMTEDEYNEFLESEAE"
     gene            complement(1964..>2499)
                     /locus_tag="PKJ65_05360"
     CDS             complement(1964..>2499)
                     /locus_tag="PKJ65_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNZ41293.1"
                     /translation="RTGYTGEDGFEIYMPASNTEKLFRKFMESGATPCGLGARDTLRF
                     ESALPLYGHELSEDITPLNAGLKSFVKLEKSDFIGKAALVEQNEKGVSVRSAGLEMVD
                     RGIARNGYDVFNEEGEKIGYVTSGSNCPSLGKNCALALISTEYTKAGTFVYVDIRGKK
                     IKAVTVKKPFYKKMYKK"
BASE COUNT          633 a          636 c          435 g          795 t
ORIGIN      
        1 aggatgtaat gaagtaaatc cctgaaggcc tgcaacatcc tcattgatct tcggattata
       61 cttcattgta caagaaccta aaggataaaa tccctgatcc acaccgtaat tatttctgga
      121 aatgttggta aagtgtctta taacatccac ttccgatact tcgggaagca aaacatcatc
      181 atctcttgtg tactttttat ctatttcata tgacggtact cggctttcgg gcagtgaaaa
      241 tgcgcatttt ccttctttag ataaattaaa tatcaattca gccattaaac tcacccgccc
      301 ttcttatgaa ctcatccatc tcttctttgg tccttttttc cgttacggct ataagccatc
      361 ctctgtcggt ttccagaccc cctataaatc cgtgtccgta aagatatcgg ttcaaagccg
      421 ctatatttac tttcggttta accgtaaact catggaagaa aggcatatcc gaagtctttt
      481 caaacttacc tgttttcagc agcccttcat aaacataatg tgcattatcc acacagcgct
      541 gtgcgatctg cttaaatccc tttttacctg tcaccgaaag ataaatggtt gccgtcaaag
      601 cacataatgc ctgattggaa catatgttgg aagtggcttt ttccctcctt atatgctgtt
      661 cccttgcctg aagggtaagt acataagcgg ttttcccgtc cacatcggta gttttaccca
      721 ctattcttcc gggcatcttt ctcatgtgct ctttaacaca tgcaaaaaat ccgagataag
      781 gaccgccgta tcctaagggc attcccaaag gctgaccttc tcccactacg aaatccgcac
      841 cgaaatcttt aggcgatttc agcagtgcca gcgccacggg atttgcacag acaataaata
      901 aactgccgct ttgttttaca tagtccccga tactctgcat atcatatata tctccgtaga
      961 aagaaggata ctgcacacat actcccgcta cgtttgttaa atcggcattt aataactgct
     1021 taaccgtcat tgatgtatat tttatatctc tgaatttcag atatgtttcc acggtctctc
     1081 tgtactccgg atgcatatcc tctacaatga ttatgtcgct tcttttacgc aaagcgcctg
     1141 aggatattac acatgcttcc gccagagccg atgcaccgtc atacatggaa gcattggcaa
     1201 cgtccattcc tgtaagttcg catatcattg tctgatattc aaagatagcg gtaagtgttc
     1261 cctgacttat ttccggctga tacggggtat atgccgttaa gaactcctgg cgtgatataa
     1321 ggtgagatat aaccgaaggt atatagtggt cataagcgcc tgcacccaaa aaacatgcat
     1381 aattgtcggt gttgctgttt tgtgcagcca aagactgcat gtatttcata agttccattt
     1441 cgctttttgc cggcggcaaa ttcaaaggtc tttttagcct gacttgctcg ggaatgcaag
     1501 aaaaaagatg ctcgacactg tcggccttac atcttttcaa catttcctgc cggtccctgt
     1561 cagttaccgg tatatatctc attattccgc ctcgctttcg aggaactcgt tatattcgtc
     1621 ttccgtcata agatcattca gttccgattc atcatcaata attatcttag ccaaatatgt
     1681 gccgtacgga tcttcattta tcttttccgg agcatcttcc agctcggcat tggcttcaat
     1741 aaccgtcccg cttaccggca tgtatacttc cgccgcggtt tttaccgatt caattgcaca
     1801 aagaacatcg ccttgctgca gtacgagatc ttcttccgcc aactcgacat aaacgatatc
     1861 tcccaactgt tgctgggcat agtcggttat gccgatatac gcatatttgc cttctttttt
     1921 aatccattca tgatcccttg taaatctcat atcccactcc ttatcatttt ttatacattt
     1981 ttttataaaa cggtttcttt accgttactg ctttaatctt tttccccctt atatccacat
     2041 aaacaaatgt acctgctttg gtatactccg tggagataag agccaatgcg cagttcttac
     2101 ctaaactcgg acaattactg ccgcttgtta cataacctat cttttcgccc tcttcattga
     2161 atacgtcata gccgtttctt gctatacctc tgtctaccat ttccaaacct gcagaccgaa
     2221 cggacacacc tttttcgttt tgttcaacca gagcggcttt tccgataaaa tcgcttttct
     2281 ccagtttaac aaagctcttg agccccgcat tgagcggtgt tatatcttcg gacaactcat
     2341 gtccgtacaa aggcaatgca ctctcaaacc gcaatgtatc ccttgctccc agaccgcacg
     2401 gggtagcacc tgattccatg aattttctga acagtttttc cgtgttgcta gcaggcatat
     2461 atatctcaaa gccgtcttct ccggtatagc ccgttctgg
//
LOCUS       DAOPWC010000226         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_44189_length_2491_cov_2.861248, whole genome shotgun sequence.
ACCESSION   DAOPWC010000226 DAOPWC010000000
VERSION     DAOPWC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44189_length_2491_cov_2.861248"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1994)
                     /locus_tag="PKJ65_05365"
     CDS             complement(159..1994)
                     /locus_tag="PKJ65_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNZ41294.1"
                     /translation="MTGYDWENISRDFDINRDIYNSIKDKTDEFIISYHDSAKWKAGW
                     GHDFHCDYCSAALAFNIQRSESYICPVCGKENRGDRKDWAWTYMYRSTSCVYVKYAGI
                     LYNITKKYGYINYIKTVMNFLTDNYEIFESFAPNLRFMGRYSGTGLSDAINTMYLLYG
                     IDAVSDYLTKEELDRWHEGFFTPQADMFDLFANKIYNIPLWQKVSEANIGVFFKDKAI
                     IDRAFYSRFGILDQLRRGVTKDGMWFETSTGYHFYGLDGVADLLLLCQRNQVFIPEHE
                     EMMSIAESQFMFPLKIMFRNGCIANPADSGLDMSIFRYNHMYVKACALFDNPYFKQVL
                     GYIKYKRDFPRTLEEILYGVKYEKCKQLPDFGSNNFEDSFCVMLKNDVTELYLKTGLK
                     DICHAHPDVMTMELAFYGDLVAKDIGSAGYSSHIFYEWQRKTVAHNTVVVNRTDQTYT
                     PVGEGIWPEGIIEYFDDNHVIAKSKNVYECVDYTRDMKIDDNKVYDKFNIKSFEELET
                     DYVFYCKGKCIIDYKYEEVQSIGDKEGYQHLFDIKKIDNEKPVTVKFDMEDKVVNVTV
                     QPEEGSVVYVVNSYVNNFNDTRYGIIVRRKGKSGTYKVMYECVLK"
     gene            complement(2190..>2491)
                     /locus_tag="PKJ65_05370"
     CDS             complement(2190..>2491)
                     /locus_tag="PKJ65_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ41295.1"
                     /translation="LRTAKGGLYEALAADFLYKKGYDKLYFYRNDSGTVEMEFLLPSN
                     DGLIPLEIKAGRNATPSLNRILESYDVPYGIKFSSQNVGVSGKKITLPIYMMMFI"
BASE COUNT          748 a          542 c          386 g          815 t
ORIGIN      
        1 ttacaaaggt atttttaata tgtcaatacc atatattgaa tattttaact ttttatatta
       61 attttcttaa ttttccgctt taactatgtg aaaaaacaaa catatgtttt gtgctgcgaa
      121 aaaatattac ccggcactgc cgggtaattc aatctaaact atttcaaaac acactcatac
      181 atcactttat atgtcccgga ttttcctttt cttctgacaa taatgccgta tcttgtatca
      241 ttgaaattgt tgacatagct gtttaccaca tacacaaccg acccttcttc cggctgcaca
      301 gtaacattga caactttatc ttccatgtca aactttacgg taacaggttt ttcgttgtca
      361 atcttcttaa tatcaaacag atgctggtat ccttctttat ctcctatcga ctgtacttct
      421 tcgtatttat aatctataat acattttcct ttgcagtaga acacatagtc cgtttccagt
      481 tcttcaaaag attttatatt gaatttatca tataccttgt tatcatcaat cttcatatcg
      541 cgggtatagt ccacacattc atatacgttt ttggatttgg ctatgacatg attatcgtca
      601 aaatattcaa ttatgccttc aggccatatt ccctctccta cgggagtata ggtctgatcg
      661 gtccggttta ctaccactgt gttatgagca acggttttac gctgccattc atagaatata
      721 tgtgaactgt aaccggcgga acctatatct ttggcaacca aatcaccgta aaaagccagt
      781 tccatagtca tgacatcggg atgagcatgg cagatatcct tcagaccggt cttgagatac
      841 agttcggtaa catcattttt caacataaca cagaatgaat cttcaaaatt attggagccg
      901 aaatcgggta actgcttaca tttctcatat ttgactccgt aaagaatttc ttctaaagtt
      961 ctgggaaaat ccctcttata ctttatatat cccagcacct gcttaaagta gggattatcg
     1021 aaaagagcac atgctttaac atacatatgg ttatatctga atatactcat atccagtccg
     1081 ctgtcggcag gatttgcaat acaaccgttt ctgaacataa tcttcagggg gaacataaac
     1141 tgactctccg ctatagacat catttcctca tgttccggaa taaaaacttg gtttctttga
     1201 caaagcaaca gaagatcggc cacaccgtcc agtccgtaaa agtgatagcc ggtacttgtc
     1261 tcaaaccaca taccgtcctt ggtcacacct cttcttaact gatccagtat tccgaatcgg
     1321 gaataaaatg ccctgtctat aattgcctta tccttgaaga atacccctat gttggcttcg
     1381 cttactttct gccaaagagg tatgttgtat atcttattgg caaataaatc aaacatatcg
     1441 gcctgcggag taaaaaaacc ttcatgccat ctgtccaatt cttcttttgt aagataatcc
     1501 gaaaccgcat caatcccgta cagcaaatac atggtattta tggcatcgga taatcccgtc
     1561 ccggaatatc tgcccatgaa tcgcaagttg ggagcgaaag attcgaatat ttcataattg
     1621 tccgtaagaa agttcattac cgtttttata tagttgatat aaccgtactt tttggtaata
     1681 ttgtaaagaa taccggcata ttttacatat acacaacttg tgcttctgta catataagtc
     1741 catgcccagt cttttctgtc acccctgttt tccttgccgc atacgggaca aatatatgat
     1801 tcgcttcttt gaatattgaa agctaatgcg gcggagcaat aatcgcaatg aaagtcatgc
     1861 ccccatcctg ctttccactt tgcactgtca tggtatgaaa taataaactc gtctgtttta
     1921 tctttaatag agttatagat atcgcgattg atatcaaagt ctctgctaat attttcccag
     1981 tcataccctg tcatagtttt atcctcctat gccgtattac tgacatattg cagcctttaa
     2041 cagctgcaac ggctccacta tagcagagct tcatctcagt ttcaacatca aaatgcaaag
     2101 ttcacaaaaa gttctcaatt aatattcaga caactgttta tatatatcaa ataagtacaa
     2161 taaaagaatc taatcataca aatattgtgt tatataaaca tcatcatgta gataggaagt
     2221 gttattttct ttcccgatac ccctacattc tgtgaagaaa actttatacc gtaagggaca
     2281 tcataactct caagaattct gtttaatgac ggtgtagcat tacgcccggc cttaatctcc
     2341 aaaggtatta agccgtcatt tgaaggcaat aaaaattcca tctcaacggt accggaatca
     2401 ttgcggtaga aatacagctt atcatatcct tttttgtaaa gaaaatccgc cgctaaagct
     2461 tcataaagac cgccttttgc cgttcttagc a
//
LOCUS       DAOPWC010000227         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_44331_length_2485_cov_3.881893, whole genome shotgun sequence.
ACCESSION   DAOPWC010000227 DAOPWC010000000
VERSION     DAOPWC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44331_length_2485_cov_3.881893"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKJ65_05375"
     CDS             complement(<1..320)
                     /locus_tag="PKJ65_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41296.1"
                     /translation="MKKMIAVLLILILLLTGCTDVTQPSPQPSAVVRDESIKSIESLS
                     QLKSLLDASKDNIFSRWYRSVGSALLAKDANGPDYAVESVTATGDASQSHSETNTQVE
                     GVDEA"
     gene            483..1079
                     /locus_tag="PKJ65_05380"
     CDS             483..1079
                     /locus_tag="PKJ65_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNZ41297.1"
                     /translation="MDINKVLENLRKNNMNAVFAENRDRAKELVKEMLFVDASITTGG
                     SVTLDQCGITKMIRENRYRLIERADKAGMFSADFMLSGTNAITEDGKLYNVDGASNRV
                     ACLCHGPKKVIVVAGINKIVKDIEQAVLRVKHIAAPKNCMRLSRNTPCAHTGKCISDD
                     INKTCDSADMICCNYVISAKQREKGRITVIIVNEELGY"
     gene            1060..1512
                     /locus_tag="PKJ65_05385"
     CDS             1060..1512
                     /locus_tag="PKJ65_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNZ41298.1"
                     /translation="MRNWDTDTLRKVRERLLNDIKKAWIPAVACIAYVVVMILAVGEV
                     CPSKLLFGVPCAGCGMVRAFALLITFRFKEAFIMHPGIYLMLIAFIIFIIMRYFLNSD
                     AKRIKILVTVTLSLLFVIFVVRVATSFGTEPLTVYNNAVLLRFIRLFY"
     gene            1562..2026
                     /locus_tag="PKJ65_05390"
     CDS             1562..2026
                     /locus_tag="PKJ65_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41299.1"
                     /translation="MTETNNQQNNQETQETLEMQNNNVVEETVVDDMQQNNEQQEYQQ
                     ETYQQQTYQQPVYQQPAVTKPGTSKTISILDWIFTIIVMSIPVVNIIMLFVFAFAGEK
                     ESKKNLFKAQIILMATVVIAFIVFLIVFVVFMGHRLPELLDQLNQFREQYGY"
     gene            complement(2142..>2485)
                     /locus_tag="PKJ65_05395"
     CDS             complement(2142..>2485)
                     /locus_tag="PKJ65_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41300.1"
                     /translation="PQWDADQIQINAANLKRRYDVFHEYNDGRLYILTGEYEQPTNPE
                     YTNHISEWAKAGMLYVSSVMVDQVEPKVALKAYLETVMAFNPQATLDFMNAKLGKTSN
                     QNYNEVWNSLN"
BASE COUNT          763 a          454 c          525 g          743 t
ORIGIN      
        1 gcttcatcca ctccctccac ctgtgtattg gtctcggagt gtgattggct cgcatcaccc
       61 gtcgctgtta ccgattctac tgcatagtcc ggtccgtttg catccttggc taacagagca
      121 ctgcctaccg atctgtacca tctggagaaa atattgtctt tggatgcatc cagcagtgac
      181 ttgagctgag aaagactttc aatgctttta atactttcat ctctcacaac agccgaaggt
      241 tgcggtgacg gttgagtaac atccgtacaa ccggtcagca gtaatatgag aattaacaaa
      301 acagctatca tttttttcat ttttatatac cgtccttttt aacttacagc atttagacgg
      361 tcagcaatct ataaaagttc tataaaagtt tcaaaagaac ctgaacgggt ttaagtgaac
      421 ttagaggttc tttgctgaat tactgttgta aatgatataa ttaaatagaa aggagaagag
      481 aagtggatat aaacaaagtt ttggagaatt tgagaaagaa taacatgaat gccgtttttg
      541 cggaaaacag agaccgtgcc aaagagctgg ttaaagaaat gcttttcgta gatgcttcaa
      601 ttacaacggg aggatcggtt actcttgacc aatgcggaat aacaaaaatg attcgtgaaa
      661 accgctatcg cttaattgaa agagccgata aggcaggcat gttcagtgcg gattttatgc
      721 tttccggtac aaatgccatt accgaagacg gtaaactgta caatgttgac ggtgcgagca
      781 acagagtggc ttgtttatgt cacgggccta aaaaggtaat agtagttgcg ggaatcaata
      841 aaatagttaa ggatattgaa caagctgttt tgagagtaaa acatattgcg gcaccgaaaa
      901 actgtatgag attgagcagg aataccccct gtgcccatac gggaaaatgc atatccgatg
      961 atattaacaa aacctgcgac agcgccgaca tgatttgctg taactatgta atatcggcta
     1021 aacaacggga gaagggaaga ataacggtaa taatagttaa tgaggaactg ggatactgat
     1081 acattaagaa aagtgcggga acgactgctt aatgatatca aaaaagcatg gattcccgca
     1141 gtggcatgta ttgcatatgt ggttgtaatg atacttgcgg taggagaggt atgtccgtcg
     1201 aaattgcttt tcggggtacc gtgtgccgga tgcggaatgg tgagagcttt tgcgcttctt
     1261 atcacattca ggtttaaaga agcttttatt atgcatcccg ggatatatct tatgttaatt
     1321 gcattcatta tattcattat catgagatac ttcctgaaca gcgatgcaaa acgtattaag
     1381 attctggtaa ccgtgactct gtcgctcctg tttgtcatat ttgtagtaag ggtggccaca
     1441 tcattcggta ccgaaccgct aacagtatac aataatgctg tattgttgcg atttataaga
     1501 ttgttttatt gacaatattt catctcataa tataatttaa cttgaaaagg agggattaaa
     1561 aatgactgaa actaataatc aacagaataa ccaagagaca caggaaactt tggaaatgca
     1621 gaacaataat gtggtagaag aaaccgtcgt tgatgatatg cagcaaaata atgagcagca
     1681 ggaatatcaa caggaaacct atcaacagca aacttatcag caaccggtct atcaacaacc
     1741 tgccgttacc aaacccggca catcaaagac tatttccata ctggactgga tattcactat
     1801 tattgtaatg tcaatacctg tcgtcaatat tattatgctc tttgtttttg cttttgccgg
     1861 agaaaaggaa agcaagaaaa atcttttcaa agcccagatt attcttatgg ctacagtcgt
     1921 gattgctttt attgtattcc tgatagtatt tgtggtattt atgggacaca gacttccgga
     1981 gttgttggat caactgaatc aattcagaga gcagtacggt tactagtcga tatttgctat
     2041 tgaacagtaa gagatacagt tgataaaaaa cttaagatac aaagacaaaa aagaacccgg
     2101 taacccgggt tctttggtta ttcggttttt ttacctgtta attagttcag gctgttccat
     2161 acttcattat aattctgatt ggaagtcttt cctaatttag cattcatgaa gtccaatgtt
     2221 gcctgaggat tgaacgccat aacagtctca agataagcct tgagagctac tttgggttca
     2281 acttggtcaa ccattacgct ggatacatac agcattcctg cttttgccca ttcgcttata
     2341 tgattggtat attccggatt tgtgggctgc tcgtattcac ccgtaagtat gtataatctt
     2401 ccgtcattgt actcgtggaa tacatcgtat cttctcttaa gatttgctgc atttatctgt
     2461 atctgatcag catcccattg cgggt
//
LOCUS       DAOPWC010000228         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_44442_length_2481_cov_2.998763, whole genome shotgun sequence.
ACCESSION   DAOPWC010000228 DAOPWC010000000
VERSION     DAOPWC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44442_length_2481_cov_2.998763"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKJ65_05400"
     CDS             <1..552
                     /locus_tag="PKJ65_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ41301.1"
                     /translation="TRRSLNVPGLRDLEGKGISYCVTCDGFFFRNKKAAIIGYTDYAE
                     HELNNLLMFTKDVYLLTNGNNPEFDVPGEIPVIDKQISGIYGRDSVEGVEFKDGSKQQ
                     FDGIFIAYGTADASSFSLKSGIATEKGKIVVDSSKMTNIDRIYAAGDCINTYMQVATA
                     VSDGALAARSVTERLKEDRKRSK"
     gene            552..2480
                     /gene="metG"
                     /locus_tag="PKJ65_05405"
     CDS             552..2480
                     /gene="metG"
                     /locus_tag="PKJ65_05405"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ41302.1"
                     /translation="MKKFYITTAIAYASGKPHIGNTYEIILADSIARFMRMLGYDVFF
                     MTGTDEHGQKIEEKAKEQNITPKQFVDGWAKEIKRIWDLMNTSYDKFIRTTDADHEQQ
                     IKKIFKKLYDKGDIYKSQYEGWYCTPCESFWTQSQLEDGKCPDCKREVNKAKEEAYFF
                     RLSKYQDRLMEHIERNPDFIQPESRKNEMVNNFLKPGLQDLCVSRTSFKWGIQVPFDP
                     KHVVYVWIDALSNYITGIGYDADGNHSELFNKYWPCDVHVIGKDIVRFHTIYWPAMLM
                     ALDVQLPKTVLGHPWLLFGDGSKMSKSKGNILYADDLSALFGVDEIRYYVLSEMNYAN
                     DGIISHRQIIEKINSDLANVLGNLVNRTVTMQHKYFEGTVKKPASLLPENQADRELIE
                     LTESTPDKVCDLMMRYKVSEAIHEIMTLAKRCNKYIDETLPWTLFKNEEDHERLKKVM
                     YNLLEGIRVVSVLLYPFMPSTSDKIISQLNTKETTFASTYTFGKLEDGIELRKPEILF
                     NRIDTEKKLLEIEKTNNEKEQEQDLNVALIGIEDFAKVELKTAKIVSCEPVKGAKKLL
                     KLQVETDGKTRQIVSGIAEYYTCDELIGKTVVVITNLKPAVLRGETSEGMLLAASDKD
                     RLSLVTIDREMPTGSKIS"
BASE COUNT          846 a          449 c          570 g          616 t
ORIGIN      
        1 accagaagaa gtctcaatgt gccgggctta agagaccttg aagggaaggg tataagttat
       61 tgcgtgacat gtgacggttt tttcttccgc aataaaaaag cagcgataat aggatatacc
      121 gattatgcag aacatgaact taacaatctg ttgatgttca caaaagacgt atatctttta
      181 accaacggaa ataatccgga atttgatgtg ccgggcgaaa taccggtaat tgataagcag
      241 ataagcggaa tatacggtcg ggacagtgta gaaggtgttg agttcaaaga cggttcaaag
      301 caacaatttg acggaatatt cattgcatac ggtacggcgg atgcttcctc attttccttg
      361 aaatcgggta ttgctacgga aaagggaaag attgttgtag attcatccaa gatgaccaat
      421 atagacagaa tatatgctgc gggagactgt ataaatacct atatgcaggt tgccacggct
      481 gtttccgacg gtgcgcttgc cgcccgttcg gtaacggaaa gactgaaaga agacagaaaa
      541 aggagtaagt aatgaaaaaa ttctatatca ctaccgcaat agcatacgca tcgggaaaac
      601 ctcacatagg aaacacctat gaaataatac ttgccgacag catagcaaga tttatgcgga
      661 tgttaggata tgatgttttc tttatgaccg gaaccgatga acacgggcag aagattgagg
      721 aaaaagcaaa ggaacaaaac attactccca agcaatttgt tgacgggtgg gcaaaggaaa
      781 taaagagaat atgggatctt atgaatacaa gctatgataa gttcatcaga actaccgatg
      841 ccgaccatga gcaacagatt aagaaaatat tcaaaaagct gtatgataaa ggggatattt
      901 ataaaagcca atatgaaggc tggtactgca ctccgtgtga gtctttctgg acgcaatcac
      961 aactggaaga cggcaagtgc cccgactgta agagagaggt caataaagcc aaagaagaag
     1021 catatttctt cagactgtca aaatatcagg acagactgat ggagcatata gaacgtaatc
     1081 cggattttat acaacccgag tccagaaaga acgagatggt caacaacttc ttaaaacccg
     1141 gacttcagga tttatgcgta tcacgtacat cctttaagtg gggaatacag gtaccgtttg
     1201 atcccaagca tgtagtctat gtctggattg atgcgttatc caactacata accggtatcg
     1261 gatatgacgc cgacggcaac cacagcgagc tgttcaataa atactggcct tgtgatgtac
     1321 atgttatagg gaaagatatt gtccgattcc acacaatata ctggcccgcc atgcttatgg
     1381 ctctggatgt gcagcttccg aaaacggttt tgggacatcc ctggctactt ttcggtgacg
     1441 gaagcaagat gagcaaatcc aaaggtaata tcttatatgc cgatgatctt tccgctcttt
     1501 tcggtgtgga tgaaatcaga tattatgtat taagcgagat gaattatgcc aatgacggta
     1561 taatatcgca cagacagata atagagaaga ttaattccga tttagccaat gttctgggaa
     1621 atcttgttaa cagaaccgtt acaatgcaac acaaatactt cgaaggcact gttaaaaagc
     1681 ccgcatcgct gctgccggaa aaccaagcgg acagagaact tatagagtta accgaaagca
     1741 cgcctgataa agtttgtgat ttgatgatgc ggtataaggt cagtgaagcc attcatgaga
     1801 ttatgacttt ggcaaagcgt tgcaataaat atattgatga aactctgccc tggacacttt
     1861 ttaaaaacga ggaagaccac gaaagactga aaaaggttat gtacaatctt ttagaaggaa
     1921 taagggtagt ttcggtttta ctgtatcctt ttatgccttc cacttcggat aaaattattt
     1981 cacagctcaa cacaaaagaa acaacttttg cttcaaccta tactttcgga aagttggaag
     2041 acggcattga acttaggaaa cccgagatac tcttcaacag gatagatacc gaaaagaaac
     2101 ttttggagat tgaaaaaact aacaacgaaa aagaacagga gcaagacttg aatgtagcgt
     2161 taattgggat tgaagatttt gcaaaagtgg aacttaagac cgctaaaata gtatcttgtg
     2221 aacctgtaaa aggagccaag aagcttttaa aactgcaggt ggaaacagac ggtaaaaccc
     2281 gtcagatagt atcgggcata gccgagtatt atacatgtga cgagttaata ggaaaaaccg
     2341 ttgtggttat aaccaactta aaaccggccg tgctgcgggg tgagacaagc gaaggtatgc
     2401 tgttggccgc atcggataaa gacagacttt ctttagtaac tatagatagg gaaatgccta
     2461 cgggttcaaa gatatcttaa t
//
LOCUS       DAOPWC010000229         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_44728_length_2470_cov_2.898551, whole genome shotgun sequence.
ACCESSION   DAOPWC010000229 DAOPWC010000000
VERSION     DAOPWC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44728_length_2470_cov_2.898551"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="PKJ65_05410"
     CDS             complement(<1..1903)
                     /locus_tag="PKJ65_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HNZ41303.1"
                     /translation="MYKDQDHIRKFLNDMDIMRFTDVSDVSDIYIKEVPGKINLMALK
                     ETDISAMQPYKSGTIYETEGNKYHIFKFKLRVSPVYDRKPFVLKVITGFGTDWNVFRN
                     PNFLLFINGQPEQAFDFGHRECIITDCAKAHDEYDCFLFEFSGMKKGPVQLTLQTCSL
                     DYPTDELYYNILCPGESIPFIESEAIRKDTAKYLINAINMVDLREPYSDKYYETVSKA
                     NGYLLDEYYGKYCDDDTSEVTVAAVGHSHIDVAWRWPIKETRQKTVRTFTSQLKNIRD
                     YKDFRFIVSQPQLYKYLEEDYPELIDKIKLAVESRQWEPEGAMWVEADCNLTSGESLV
                     RQIMHGKKYFKDMFDRDCRILWLPDVFGYSAALPQILKKSGIDYFSTSKISWNEYNVI
                     PYNTFMWEGIDGTEIFTNFIVGGKGPNEEGRPRVTYNSWVNAEQIMGTWKNYRDKSLA
                     KYPFMSYGLGDGGGGPGRDHLEQAIRFSRGIKGMPRFESVTVTEYFDKLYDLVKDDSR
                     LPKWVGELYLEFHRGTYTSVAKNKKNNRKSEILMRDTEAFCSLADRFGLDTYDKKKIY
                     DTWETILLNQFHDIIPGSSIKEVYDDSDADYARVMKDGKELLSGSLNAFAKTVKGQDL
                     CVVFNQLAFK"
     gene            complement(1913..>2470)
                     /locus_tag="PKJ65_05415"
     CDS             complement(1913..>2470)
                     /locus_tag="PKJ65_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41304.1"
                     /translation="PETIQTNIYRANDIENYDTCVCRVITRNDVEILYYASHSTDIKC
                     EPLLHFQFEKGEIYADFNKEKCIYGKVGGKVISYGNPFELEMKKVFDCIRTLKGADVN
                     YCPIEAAIPQLKTINAISQFYDIEQVEESDLGYLDAEENTTKLVYVKGLYQDMMKAYS
                     DNLILDESAKVIDITGYKYFENMKQ"
BASE COUNT          634 a          589 c          427 g          820 t
ORIGIN      
        1 gcttgaaagc aagttgatta aatactacgc acagatcctg acctttaacg gtctttgcaa
       61 aagcattgag cgagcctgac agaagctctt tgccgtcttt catgactctt gcataatccg
      121 catcggaatc gtcatatact tctttaatgg aggatcccgg tattatgtca tggaactggt
      181 ttaacagtat tgtctcccag gtatcataaa tcttcttctt gtcgtatgta tccagcccga
      241 atctgtcggc taaagagcag aaagcctcgg tatcacgcat aagtatctcg gactttctgt
      301 tattcttctt gtttttggct accgatgtat atgtaccgcg atgaaattcc agatacagct
      361 ctcctaccca tttaggcagt cttgaatcgt cttttaccaa gtcatacagt ttatcgaaat
      421 attcggtcac cgttacactc tcaaatctcg gcataccttt tatacccctg gaaaatcgga
      481 tagcctgttc aagatgatct ctgcccggtc ccccgccgcc gtctcccaat ccgtatgaca
      541 taaacggata tttggctaag ctcttatccc ggtagttttt ccatgttccc attatctgtt
      601 cagcatttac ccaggagtta tatgttactc tcggtcttcc ttcctcattg ggaccttttc
      661 cgcccactat aaaattggtg aatatttccg ttccgtctat tccttcccac atgaaagtgt
      721 tatagggaat aacattgtac tcattccagg atattttgga tgtggaaaaa tagtctattc
      781 ccgatttctt taaaatctga ggcagtgcgg cactgtaccc gaaaacatcg ggaagccata
      841 aaatcctgca gtccctgtca aacatatctt tgaaatattt ttttccgtgc attatctgcc
      901 gcacaagaga ttctccgctg gtaagattac agtcggcttc cacccacatc gcaccttccg
      961 gctcccactg tctgctctcc accgccagct ttatcttatc tatcaattcg ggataatcct
     1021 cttccagata tttatacagc tgaggttgac ttactatgaa cctgaaatct ttgtaatcac
     1081 gtatattctt caattgtgat gtaaaggttc tcacggtctt ttgtctggtt tcttttatag
     1141 gccatctcca ggccacatct atgtgcgaat gtcctacagc ggcaacggta acttccgatg
     1201 tgtcatcatc acaatattta ccgtaatact catccaaaag atacccgttt gctttactta
     1261 cggtttcata atatttatcc gagtaaggtt ctctcaaatc caccatattt atggcgttaa
     1321 tcaggtattt tgctgtgtct tttcttattg cttcgctttc aatgaacgga atggactctc
     1381 ccggacagag gatgttgtaa taaagctcgt cggtgggata atccagcgag caggtctgta
     1441 atgtaagctg taccggtcct ttcttcattc cggagaactc aaataaaaaa cagtcatact
     1501 cgtcatgtgc cttggcacag tcggttatta tgcattcacg atgtccgaag tcaaaagcct
     1561 gctccggctg tccgtttata aaaagcagga agttgggatt ccgaaaaaca ttccaatcgg
     1621 tcccgaatcc cgttataact ttaagaacaa acggttttcg gtcatatacg ggacttactc
     1681 ttagtttaaa cttgaatatg tgatatttat taccctctgt ctcatatatg gtaccgcttt
     1741 tatacggttg cattgcagaa atatcggtct ctttcaatgc catcaggttt atcttgcccg
     1801 gcacttcctt tatataaatg tcggaaacat cggaaacatc ggtaaacctc ataatatcca
     1861 tatcattaag aaacttcctt atatggtcct gatctttgta cataataccc tcctattgct
     1921 tcatattctc aaaatattta tatcctgtaa tatcaataac ttttgccgac tcatcaagaa
     1981 tcaaattgtc gctgtatgcc ttcatcatgt cttgatacaa ccctttgaca tataccagtt
     2041 tggtggtatt ctcctcggca tccagatatc ctaaatcact ttcctccacc tgctctatgt
     2101 cataaaactg gctaattgca tttattgttt taagctgcgg tatagccgcc tctatggggc
     2161 aataatttac atcggcccct tttagtgtcc ttatacagtc aaataccttc ttcatctcca
     2221 actcaaacgg atttccgtag gatattacct taccgcctac ttttccgtat atgcattttt
     2281 ccttattgaa atcggcatat atctcgcctt tttcaaactg gaaatgcaaa agaggttcgc
     2341 actttatatc tgtggaatgg cttgcataat acaatatctc cacatcattg cgagttataa
     2401 ccctacaaac gcatgtatcg taattctcaa tatcatttgc tctgtatatg ttggtctgta
     2461 tggtttcagg
//
LOCUS       DAOPWC010000230         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_44782_length_2468_cov_2.957729, whole genome shotgun sequence.
ACCESSION   DAOPWC010000230 DAOPWC010000000
VERSION     DAOPWC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44782_length_2468_cov_2.957729"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKJ65_05420"
     CDS             complement(<1..385)
                     /locus_tag="PKJ65_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ41305.1"
                     /translation="MIIEFWEFLVNIIYSAINMLNSASVWMVVSFGIAGIVHEYMSPE
                     KMRKSSVGSTKISGVLWTTLAGTGVPICSCGSTPLGISLYYSGAYLGPTLAFMTSTPV
                     LNPVAIILTWGLLGKEVAIINIITGL"
     gene            complement(382..573)
                     /gene="saoT"
                     /locus_tag="PKJ65_05425"
     CDS             complement(382..573)
                     /gene="saoT"
                     /locus_tag="PKJ65_05425"
                     /inference="COORDINATES: protein motif:HMM:NF040730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HNZ41306.1"
                     /translation="MKSLEEQYKEQIQVKIYKVGEDFEYIKKYGPTTKSMLIINESRV
                     VTKLSKEAIRKAFEEALKD"
     gene            841..1608
                     /locus_tag="PKJ65_05430"
     CDS             841..1608
                     /locus_tag="PKJ65_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNZ41307.1"
                     /translation="MYFTGIDIGSTSAKVVALNENNDILYKNVMPTGWSSTDTAEKIR
                     KDLSIGGIDGGNSKFIATGYGRISVPYADKTITEITCHGKGAAFLYDVDTCTVIDIGG
                     QDTKIISIAKGQVVDFIMNDKCSAGTGRFLEIMASTLGMDLAELCNMAKTGSGISISS
                     MCTVFAESEVVSLIGSGHSKKDIAYGIIESITNKVISLCGKHAATNNKFYLTGGLCES
                     DYIIESLSEKLGSKVISSPLGRYAGAVGAALSAKSLK"
     gene            1626..>2468
                     /locus_tag="PKJ65_05435"
     CDS             1626..>2468
                     /locus_tag="PKJ65_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNZ41308.1"
                     /translation="MSNIDLPSKFEEFADARRQGFLKVKEIKDKGGKIAGVFCTFTPS
                     EILDAAGLFSVGLCGMSEETIIDAEADLPKNLCPLIKSSYGFALTDKCPYTYFSDIII
                     GETTCDGKKKMYELLGEIKDTYIMQLPQGVDREYAHKMWESELYRLIKTLEEKFQVKI
                     TEEKLRKAAALRNKQRNLYNELFELGKLDPPPIKGFDIYKVIEGSGFSFDVEGNCMRL
                     RELIDNTKKAYEAGNRPVAAGAKRILVTGCPIGGVLDKVVNTIESNGGAVVCFENCGG
                     VKPVR"
BASE COUNT          790 a          462 c          532 g          684 t
ORIGIN      
        1 taagccctgt aataatatta ataattgcaa cttctttccc caataatccc caggtcagga
       61 taattgcaac aggattaagc accggagtgg atgtcataaa tgccaaagtg ggacccaaat
      121 aagcaccgga atagtataag ctgattccca aaggggtgct gccgcagctg caaattggta
      181 cgcccgtgcc tgcaagcgtt gtccagagaa caccggaaat tttcgtactg ccgacactgc
      241 ttttgcgcat cttttccgga ctcatatact catgaactat tcctgcaata ccaaagctca
      301 caaccatcca tacagatgca ctgttcaaca tattgatagc agaataaata atattgacta
      361 agaactccca aaattctatt atcaatcctt taatgcctcc tcaaatgctt ttcgtatggc
      421 ttctttgctt aattttgtca ctaccctcga ttcattgatt atgagcatgc tcttggtagt
      481 aggtccgtat ttttttatgt attcaaagtc ctctccgact ttatagattt tcacttggat
      541 ttgttcttta tattgttctt cgagagattt cacgaattct ccaaatactt cgcatcatgc
      601 acaagcactg atatactcga taaaaggctt attcatcgtt cttccccccc tttaagttga
      661 taaagaacaa aagcggcttc gccgccaaga aagcctacac actcaaatat atcaataaaa
      721 gtgaccaatt tctacaaata ttaccaaata tgatagacag atattccatt ataaaaaata
      781 gcaataatgt gcattaaata tttcatgtat aatttagtag gagaaaggca ggagataaaa
      841 ttgtatttta ccgggattga tatcggttca acctctgcaa aggttgttgc cctaaatgaa
      901 aacaacgata tactttacaa aaatgtaatg ccaaccgggt ggagcagcac ggatactgcc
      961 gagaaaatca gaaaggatct atcaataggc ggtattgacg ggggaaacag caaattcata
     1021 gcaaccggct acggcagaat ctctgttccc tatgcggata aaaccataac ggaaataacc
     1081 tgtcacggta aaggagcagc atttttatat gacgtggata cttgcacggt aatagatatt
     1141 ggcggtcagg atacaaaaat tatatctatt gccaaaggtc aggttgtcga ttttattatg
     1201 aatgacaagt gttctgccgg tacaggccgg tttttggaaa ttatggcaag cacattgggc
     1261 atggatttgg ctgagttgtg caatatggcc aaaacgggct caggtataag tatcagctcc
     1321 atgtgtacgg tttttgccga gtctgaagta gtgagcctga ttggcagtgg tcactcaaag
     1381 aaggatattg catacggaat aatagaatcc atcacaaaca aggtaatatc actgtgcggc
     1441 aagcatgctg ccacgaataa caaattttat ctgaccggtg gtctttgtga aagtgattat
     1501 attatagaat ccctatctga aaagttaggg tccaaagtaa tttcaagtcc cttaggtcga
     1561 tacgccggtg cagttggagc cgcactatcg gcaaaatctc taaaataata ttaggagggt
     1621 atgttatgtc aaacatagat ttgccaagta aattcgaaga atttgctgat gccagaaggc
     1681 aaggcttttt aaaagtaaaa gaaataaaag ataaaggggg taagattgcc ggcgtttttt
     1741 gcacttttac accatctgaa atattggatg cagcgggtct tttctctgtg ggattatgcg
     1801 gcatgagcga agaaactata attgatgccg aagcagacct tccgaaaaat ctgtgccccc
     1861 tgatcaaatc cagttatggt tttgcattaa cggacaaatg cccttatact tatttttccg
     1921 atattattat tggtgagaca acctgcgacg ggaaaaagaa aatgtatgag cttttaggtg
     1981 aaatcaagga tacatatatt atgcaactac ctcaaggggt tgacagagag tatgctcata
     2041 aaatgtggga aagtgaattg tatcgtttga taaagacttt ggaggaaaaa ttccaggtca
     2101 aaattactga agaaaagctt agaaaggctg cggctctgag aaacaaacaa aggaatctat
     2161 acaacgagct ttttgaatta ggcaaattag accctccgcc aatcaaaggc tttgatatat
     2221 acaaggtgat tgaaggctcg ggcttcagct ttgatgtgga gggaaattgc atgaggcttc
     2281 gtgaactgat agacaacaca aaaaaagctt atgaagccgg taacagaccg gttgcagcgg
     2341 gagcaaaaag aatattggtg acgggttgtc ccataggtgg agttcttgac aaggttgtaa
     2401 atactattga aagcaatggc ggagcagtcg tttgctttga aaactgcggc ggtgtcaaac
     2461 ctgtaagg
//
LOCUS       DAOPWC010000231         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45015_length_2459_cov_3.194260, whole genome shotgun sequence.
ACCESSION   DAOPWC010000231 DAOPWC010000000
VERSION     DAOPWC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45015_length_2459_cov_3.194260"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKJ65_05440"
     CDS             complement(<1..1030)
                     /locus_tag="PKJ65_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41309.1"
                     /translation="MQKIKRNYLPLYALSGFGPGLINLMISAYLIDGLSTAGFLKNIE
                     NWTYANKTIVMTALFSVFVLIAKIVDGVADVPLASLTDTLKTRWGKRRPAILIGYIPM
                     IITYILFCFPLEMAEHSLLNTIWFGVLLIFFFTSYTMTLVTYYGTYSEVTENDSDRHY
                     LSSWKAFFDTVQYSIGYAVIPLMVGGLNIRHIALILSPLLLTMIIPFFLLKEQSTLPE
                     DVAKRAEAGEAVEEEVPFMESIKLTIMNKGFIMWLVVFGIFFFGLQMFLAGQNVLASG
                     PMGLNSWQIAIINSAAFAPVPLMLYFYRKVMKKKGFRFAFQTAMGSFAVAMLMFSIAY
                     IKWIPNEYI"
     gene            complement(1040..2170)
                     /locus_tag="PKJ65_05445"
     CDS             complement(1040..2170)
                     /locus_tag="PKJ65_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ41310.1"
                     /translation="MFNDMKIVDCHIHYSSNIPVDEMIRILDYCDTDVANIVVVPDRA
                     KLSAVQDALILKHRLPGRIYVFASLDASNYFIKKKTLGKAMAKYALRMREAGCDGIKM
                     IEGKPQIRKMLNVPSFDAPVWEPYFKFMEDTAFPILWHVNDPEEFWDKDKIPKWAMEQ
                     NWLYDETYVNNEQQYEEVLNVLKKHPDLKIIFAHFFFLSKQLDRLSEILDKYPNVYVD
                     LTPGIEMYINFTENKENATAFFEKYQDRIMYGTDIGTKGVVDGKSASLEEEKRRADLV
                     KRYISSKADFVIDKHYSYLMHDNDFTLSPLGLSKKIQKKIFSLNFLDFMGHTPYPVDS
                     NKVLREIRKTVVKLCLMKIGDKSGTEYDFASLKKAYKQLEKI"
BASE COUNT          722 a          533 c          435 g          769 t
ORIGIN      
        1 tgatatattc attgggtatc cacttgatat atgctatcga gaacatgagc atggcaacgg
       61 caaaagaccc catagccgtc tggaatgcaa atcggaaccc tttctttttc attactttcc
      121 tgtagaaata cagcattaaa ggaacagggg cgaatgctgc ggaatttata atggcaatct
      181 gccagctgtt aagtcccata ggtcccgatg ccagtacatt ctgtcccgct aagaacatct
      241 gaagcccgaa gaaaaagatt ccgaaaacca ctaaccacat tataaatccc ttgttcataa
      301 ttgtcagttt tatgctctcc atgaaaggta cttcttcttc caccgcttct cccgcttctg
      361 cacgctttgc aacatcctcc ggtagcgtcg attgttcttt cagcaggaaa aacggtatta
      421 tcatggtaag aagcaagggt gacagtataa gggcaatatg ccggatattc aacccgccta
      481 ccattaaagg tataacggca taaccgatgg aatattgaac ggtatcaaaa aaggctttcc
      541 aggaagaaag ataatgacga tcgctgtcat tctcggtaac ttccgaataa gtaccgtaat
      601 atgttaccag tgtcatagtg taggaagtaa agaaaaagat aagcaacaca ccgaaccata
      661 ttgtgtttaa caaactgtgt tccgccattt ccaagggaaa acagaataat atgtatgtga
      721 taatcatggg tatgtaccct ataagtatag ccggacgcct tttaccccat ctggtcttca
      781 gtgtatcggt cagcgatgcc agcgggacat ccgctacacc gtcaacaatc ttggctatca
      841 atacaaacac actgaacaaa gcggtcataa caatggtttt attggcatat gtccagtttt
      901 caatgttctt caaaaaacct gccgtactca agccgtctat aagataggct gaaatcatta
      961 ggtttataag cccgggtccg aaaccggaca gagcatataa aggcaggtag tttcttttga
     1021 ttttttgcat aatatcctcc taaatctttt ccaattgttt ataagctttc ttcagtgatg
     1081 caaaatcgta ttcagtaccg gatttatctc cgattttcat taaacaaagt ttaactacgg
     1141 tttttctgat ttcccgcaac accttatttg agtcgaccgg ataaggcgta tggcccatga
     1201 aatccagaaa gtttaaagag aatatctttt tctgtatctt tttacttaaa cccagagggc
     1261 tcaatgtgaa atcgttatca tgcataaggt aggaataatg cttgtcgata acaaaatccg
     1321 ctttggaaga aatatacctt ttaacaaggt ccgctcttct cttctcctct tccaaagaag
     1381 cacttttacc gtccaccaca ccttttgtac ctatatcggt tccgtacata atcctgtcct
     1441 gatatttctc gaaaaaagct gtagcatttt ccttattctc tgtgaaattg atatacatct
     1501 ctattcccgg ggttaaatca acatatacat tcggatattt atccaatatc tcggataatc
     1561 tgtccaattg ctttgacagg aaaaagaagt gcgcaaatat tatcttcaga tcaggatgtt
     1621 tttttaatac attcagcact tcctcatatt gttgctcatt attgacatat gtctcatcat
     1681 aaagccagtt ctgctccatt gcccatttgg gtattttatc tttatcccag aactcttcag
     1741 ggtcattgac atgccataaa atagggaaag cggtgtcttc cataaactta aaatacggct
     1801 cccataccgg tgcatcaaag gaaggtacat tgagcatctt tcttatctga ggtttgccct
     1861 ctatcatctt tataccgtca catcctgctt ctctcatccg caatgcgtat tttgccattg
     1921 cttttcccaa agttttcttt ttgatgaaat agttggaagc atccagactt gcaaacacat
     1981 atatcctgcc gggtaatctg tgtttaagaa taagggcatc ctgtaccgct gataatttcg
     2041 ctctgtcggg tacaacaaca atattggcta cgtcggtatc gcaatagtcc agtattctta
     2101 tcatttcatc aacaggtatg ttggatgaat agtgtatgtg gcagtcaaca attttcatat
     2161 cattgaacat atgcgcctct taaaacaata ctttatttgc atatatataa tatcacaagt
     2221 acaacacatg tttaaggtga tgcttttaaa ttttgaagaa aaaggtgtaa agaatatact
     2281 gcatcatgca aaatatttaa ttcctcttcc tttccccgtt gtttcaatat atccttcggc
     2341 tacaagatta ttcaaaatgg aaactgcttt ggttttcccg aaacctgtcg cttttacaat
     2401 gtccgagctg gataggcgtc ttcttttcaa aagtgagtac actcgtttct catcttccg
//
LOCUS       DAOPWC010000232         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45038_length_2458_cov_3.084478, whole genome shotgun sequence.
ACCESSION   DAOPWC010000232 DAOPWC010000000
VERSION     DAOPWC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45038_length_2458_cov_3.084478"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..537)
                     /gene="rpsL"
                     /locus_tag="PKJ65_05450"
     CDS             complement(127..537)
                     /gene="rpsL"
                     /locus_tag="PKJ65_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNZ41311.1"
                     /translation="MPTFNQLVTKGRQVSKTKSLSPALQKSMNTIRKRTTAVACPQKR
                     GVCTVVKTNTPKKPNSALRKIARVRLSNGYEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDAQGVANRKQGRSKYGAKKGK"
     gene            complement(570..815)
                     /locus_tag="PKJ65_05455"
     CDS             complement(570..815)
                     /locus_tag="PKJ65_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNZ41312.1"
                     /translation="MMFTAASQDKKVIGLKQSMKAVSEDRAMTVYIAKDADRSLTEPL
                     AKLCEKKGVEIRYADTAGQLGLLNGISVKASAVCILK"
     gene            complement(812..>2458)
                     /locus_tag="PKJ65_05460"
     CDS             complement(812..>2458)
                     /locus_tag="PKJ65_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ41313.1"
                     /translation="IIKLWRKTTDDVTKMLMEGLDAFNPLNVMSRSGARGDMNQIKQL
                     AGMRGLMFDTAGKTIEIPIKSNFREGLNVLEFFISSNGARKGLADTALRTADSGYLTR
                     RLVDVSQDVIVREEDCGTEDGIYVSDIVDSGEVVVKLKDRIYGRYVTDPVIDKKTGEI
                     IVEAGGYIDADATKRIVDAGIKEVKVRSVLTCKSELGVCAKCYGMNLASSKPVNIGEA
                     VGIIAAQSIGEPGTQLTMRTFHTGGIAGDDITQGLPRVEELFEARKPKGLAIISENDG
                     IVKINESRKRREVIVTGEDESHTYVIPYGSRLTVVDEQEIKKGDKITEGSENPHDILR
                     IKGVDGVHRYIACEVQKVYKLQGIDINDKHIEIIVKQMLRKVKVEDCGDTNLIPGSYC
                     DVSDFNKQNQLAEQEEKQPATGEVVLLGISKASLATDSFLSAASFQETTRVLTEASIK
                     GKTDNLIGLKENVIIGKLIPAGTGMNAYQDPYLEMDPIDDEEDFMVEEDYEDEVDLFD
                     DMNEDEETLDLDHSEEYDDEDLSDDADSDDLSESEEEQEE"
BASE COUNT          567 a          619 c          439 g          833 t
ORIGIN      
        1 tccttttctt ggcacttgtc ttccctcctt aataataatt tttcataggt actcagccgt
       61 gtcagctgtc agtccgggta catatcacct tttatatatg ttaagtaccg gatacttcta
      121 agtacattat ttaccttttt ttgcaccgta tttggatctg ccttgttttc tgttagcaac
      181 tccctgtgcg tccaatgttc ctctgataat gtgatatctt acaccgggta agtctttgac
      241 tcttccgcct cttatgagaa caacgctgtg ttcctgaaga ttgtggccta ttcccggtat
      301 atatgctgtc acttcatacc cgttggaaag tctaactctg gcaatttttc tcaatgcgga
      361 attaggcttc ttgggagtgt tggtttttac caccgtacac actcctcttt tttgaggaca
      421 agctactgct gtagttctct tcctgatggt attcatgctt ttctgtaaag ccggtgacag
      481 tgatttcgtt ttggaaacct gtcttccttt tgttaccagt tgattaaatg tcggcatctt
      541 ttccctcctt tctttaagta gttatatatt tacttcaaaa tacataccgc tgatgcttta
      601 acgcttatac cgtttaacaa tcccaattgt ccggcggtat cggcatatct tatctcgaca
      661 ccttttttct cacataattt tgcaagcggc tcagtcagtg agcggtcggc gtctttagca
      721 atgtataccg tcattgcacg gtcttccgaa acggctttca tcgattgttt aagaccgatt
      781 acctttttat cctgcgatgc tgccgtaaac atcattcctc ctgctcctct tccgattcgg
      841 ataaatcgtc ggaatcggca tcatccgaaa ggtcttcatc atcatactct tcggaatggt
      901 caaggtcaag ggtttcttca tcttcattca tatcgtcaaa caaatccact tcatcttcat
      961 aatcttcttc gaccatgaaa tcctcttcat catctatcgg atccatctca agataggggt
     1021 cctgataagc attcatgcct gttcccgccg gaatgagttt tccgataata acgttctcct
     1081 taagtcctat cagattatcg gttttacctt taatggaagc ttcggtcagt acacgcgttg
     1141 tttcctggaa ggaagcggcg gaaaggaatg aatcggtggc aagggaagct ttacttatac
     1201 ctaacagtac cacttcaccg gtagcaggct gtttttcttc ctgttcggca agttggttct
     1261 gcttgttgaa atcacttaca tcacagtaac tgcccggtat gagatttgta tctccgcaat
     1321 cttccacctt tactttacgc aacatctgtt taactattat ctctatatgc ttatcgttaa
     1381 tatcaatacc ctgaagcttg taaactttct gtacttcgca ggcaatatat ctgtgtactc
     1441 cgtccacacc ttttatcctt aagatatcat gcggattttc cgaaccttcg gttatcttat
     1501 cgcctttttt aatttcctgc tcatcaacaa cagtcaatct ggagccgtaa ggaataacat
     1561 atgtatgaga ctcatcttct ccggtaacaa taacttccct gcgttttctg ctctcgttta
     1621 tcttaacaat accgtcattt tccgatataa ttgccaaccc tttgggtttt ctggcttcga
     1681 aaagttcctc gacacgcggc agaccctgag taatgtcgtc tcccgcgata ccgccagtat
     1741 ggaaggttct catggtaagc tgggtacccg gctcgcctat cgactgagcg gcaataatac
     1801 cgacagcttc tccgatatta acgggcttgc tggatgcaag gttcattccg tagcattttg
     1861 cacatactcc gagttccgac ttgcaggtta atacagaacg taccttaact tccttaatac
     1921 ccgcatcgac aattctcttt gtagcatcgg catctatata tccgccggct tccactatga
     1981 tttcgcctgt tttcttatct ataacgggat cggtgacata acgaccgtat atcctgtctt
     2041 taagttttac taccacttca ccgctgtcca ctatatcgga aacatagata ccgtcttcag
     2101 taccgcaatc ttcttctctt actatgacat cctgcgaaac atccacaagt cttcttgtga
     2161 gataaccgga gtcggctgtt cttaacgcgg tatcggcaag accttttctt gcaccgttac
     2221 tggaaatgaa gaattccaaa acattcaagc cttcacggaa gttggacttt atcggaatct
     2281 ctatggtctt tcccgctgtg tcaaacatca gtcccctcat acccgcaagc tgctttatct
     2341 ggttcatgtc tcctctggct cccgatctgc tcattacatt aagaggattg aatgcatcca
     2401 agccttccat aagcatcttt gtaacatcat cggttgtttt tctccacaat tttattat
//
LOCUS       DAOPWC010000233         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45116_length_2455_cov_3.473333, whole genome shotgun sequence.
ACCESSION   DAOPWC010000233 DAOPWC010000000
VERSION     DAOPWC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45116_length_2455_cov_3.473333"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PKJ65_05465"
     CDS             <1..1171
                     /locus_tag="PKJ65_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41314.1"
                     /translation="GIFNANAGRTLNENLKDNLKHSIGADISFVPSVLEYDISLEGFR
                     FVDNRGTGISKQAHKYCNSDLIENYTVYYYHNTTLKPSDQSVTFMGIETGDFGRVVKI
                     RKDLNEGVPLNHILNILTTYPKGIIISRSISQLYNVKAGQYFDFTYTNRAFREVQEIP
                     VKDVLVVAVVDYWPTMTVDNFIIMTQNKYSSMRHFRQNGKVLVDKKEGVSDNEVLEDM
                     KTRMSYDESIGTNSFTDYMATQAKRGSVLNGINGILTLDFLISMLICCIGFIIFWIIS
                     VRQRVLQFGILRAMGMKKHELYRMIFYEQLMISFVSVLAGILIGGIASELFVPLFERM
                     STFSSNFLPFYAYREIVDYNRVYIITGAALIIGLSVILRFTSKLKVDQALKLGED"
     gene            complement(1224..>2455)
                     /locus_tag="PKJ65_05470"
     CDS             complement(1224..>2455)
                     /locus_tag="PKJ65_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41315.1"
                     /translation="SFILCSCRKEAQIVPAPTNTVESDLVILQPDLSLPDEYYTSMHQ
                     GARVWHSYLNTPYGKYHYVDGFILYSQYGNSKFVKLCTKAECTHMDDNCDAYLGKIDQ
                     IVPTNEKTIQLGYYQDNIYYVERSKKNGLDWNLIKMNMDGTERQLVKTFATVSLTQGK
                     RCLGFFHSGYYYFVMINAGQEMGSKYNTDNNFYRVKLDDYSEYESVISHDVIPEACMF
                     TLVGDNVFFYVRTGPYNENYKLYDENPAEYELYMLSLTYKNMRLLTDKWAGYRNSHFD
                     WENAYCYKHNDGFYELNLTSEQMNKVADFKIEDAWGSLAYFYPDYNYIIAFAKTSDSR
                     GYPRYFDSQTLYILDKQYHILNSCIIDALKLDTGCFIDDTGTSVLLASSAHKPIDFFI
                     YKSDIGTDDVKLNEITD"
BASE COUNT          751 a          445 c          503 g          756 t
ORIGIN      
        1 aggtatattc aatgcaaatg cgggaagaac gcttaatgag aatctgaaag acaatctgaa
       61 gcattccata ggtgccgata tatccttcgt accgtcggta cttgaatatg atatctcttt
      121 ggaagggttt cgttttgtgg acaacagagg aacgggtata agcaaacagg cccataaata
      181 ctgtaattcc gatctgattg agaactatac cgtttattac tatcacaata ccacccttaa
      241 accttcggat caatcggtaa cttttatggg tattgaaacg ggtgatttcg gcagagttgt
      301 aaagataaga aaagatttga atgaaggcgt acctttaaat cacatattga acattttgac
      361 tacatatcct aaagggataa tcatatcgcg tagtatttca cagctttata acgttaaagc
      421 aggacaatat ttcgatttta cctacacaaa cagagcgttt cgggaagtgc aggagattcc
      481 cgtgaaagac gtgcttgttg ttgcggttgt ggattattgg cctacaatga cggtagataa
      541 ttttattatt atgacgcaga acaaatactc ttcgatgagg cattttaggc agaacgggaa
      601 ggtgctggtg gataaaaaag aaggcgtcag cgataatgag gttctggaag atatgaaaac
      661 aagaatgtcc tatgacgaat caataggcac aaattctttt accgattata tggcaacaca
      721 agcaaagaga ggatcggttc tgaacggaat taacggtata cttacattgg attttctgat
      781 atccatgctt atatgctgta taggtttcat tatattctgg attatatcgg tacgacagag
      841 ggtactgcaa ttcggtattc tccgtgcaat gggcatgaag aagcatgaac tgtacaggat
      901 gatattttac gagcagctca tgatttcctt tgtatcggtg ctggcgggaa tattaatagg
      961 cggtattgca agcgagttat ttgttccctt gtttgaaaga atgtccactt tcagcagtaa
     1021 ttttcttcct ttttatgcct accgggagat tgtggattac aacagggttt acattatcac
     1081 aggtgcggca ctgataatcg gtttatcggt aattttgaga tttacatcca aacttaaagt
     1141 ggatcaggca cttaaactgg gtgaggactg ataatattac cgctgaactg acggtataaa
     1201 gtttaattta tctttctgaa caatcaatcc gttatttcat tcaatttaac atcatccgtt
     1261 ccgatatccg acttgtatat aaagaaatct atgggcttgt gagcggagga tgccagtaaa
     1321 acggaggttc cggtatcatc aataaagcat cctgtatcca gcttcaaagc atcgataatg
     1381 caagagttca atatgtgata ttgcttgtca agtatataca aagtctgact gtcaaagtat
     1441 ctgggatagc ctctgctgtc agaggtcttt gcaaaagcaa tgatgtaatt atagtcggga
     1501 tagaaatatg caagagaacc ccaagcatct tcaatcttaa aatcggcaac tttgttcatc
     1561 tgttcagacg taagattcaa ttcatagaaa ccgtcattat gtttgtagca gtatgcgttt
     1621 tcccaatcga aatgagagtt tctgtagccc gcccacttat ccgtcaataa acgcatattc
     1681 ttatacgtaa gagagagcat atacagttca tactcggcag ggttctcatc atacagttta
     1741 tagttttcat tgtagggacc tgttctgaca taaaagaata cattgtcacc gacaagcgta
     1801 aacatgcaag cttcgggtat tacatcatga ctgataaccg actcatattc cgaataatca
     1861 tccaatttaa cacgatagaa attgttgtcg gtgttgtact tagagcccat ttcctgtcct
     1921 gcgttaatca ttacaaaata gtaatagccg gaatgaaaaa accctaagca tctttttcct
     1981 tgcgtcagcg acacggtcgc aaaagttttt acaagttgac gttccgtacc gtccatattc
     2041 attttgataa gattccaatc taaaccgttc ttttttgatc tctcaacata atatatgtta
     2101 tcttgataat aacctaactg aatagttttt tcgtttgtgg gaactatttg atcaattttt
     2161 ccgaggtatg catcacaatt atcatccata tgggtacatt ccgcttttgt acataattta
     2221 acaaactttg aatttccgta ttgcgaatag agaataaaac cgtcaacata atgatattta
     2281 ccgtaggggg tattcagata ggaatgccat acccgtgctc cctggtgcat ggatgtgtaa
     2341 tactcatccg gtaaggataa atccggttgc agtataacta aatccgattc aactgtattt
     2401 gtcggtgccg gaactatttg ggcttcttta cggcaggagc ataatataaa ggaca
//
LOCUS       DAOPWC010000234         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45256_length_2450_cov_3.830480, whole genome shotgun sequence.
ACCESSION   DAOPWC010000234 DAOPWC010000000
VERSION     DAOPWC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45256_length_2450_cov_3.830480"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="PKJ65_05475"
     CDS             complement(<1..1767)
                     /locus_tag="PKJ65_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNZ41316.1"
                     /translation="MYTRNSRIKTLFNAPLGKDVLDLIAYMSGKGAFLFKNPIVGNIR
                     VGRLPHLTKNQITEDFVDSLIDMLNREPDLHKSPTSELKKTWWKSAVFYQIYPRSFKD
                     SNNDGIGDLNGIVSKLDYLKYIGIDAIWLSPVYDSPNDDNGYDIRDYKKIMTEFGDMA
                     DFDLLLREVHKRGMKLIMDLVINHTSDEHEWFKEALKDKNSKYHDYYIFRKGKNNGPP
                     NNWTSAFKGSAWNYYPDLDEYALHVFSKKQMDLNWENENMRKDIYDMINWWLEKGVDG
                     FRLDVINLISKTPGLPDGLPALKGLVGAIGYEHMFYGPKLHEYLREMRKNTFDKHNAF
                     TVGEGAGIGLEAAKLMTEESRKELDQIFNFDHFNNPGKSRHDSYRYDLRKLKPLLVKW
                     QRDFKDGCWPALVFENHDNPRFVSRITKDESKRDLAAKLIITLMLTLRGTPYFYQGQE
                     IAMIDSVFKDISDFRDVEAINMFNEYLEQGLSEEQAILKISAGTRDHARIPMQWEDAA
                     YGGFSEVKPWIDCSNDYMRYNAKSQIKDTFSVLNYMKSLISFRKENQTLIYGDFILTD
                     NDADTFCYERKDKDARFYIEINL"
     gene            complement(1925..>2450)
                     /locus_tag="PKJ65_05480"
     CDS             complement(1925..>2450)
                     /locus_tag="PKJ65_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41317.1"
                     /translation="FYPQDEYLYIYTYSPDKKYSVCRYSMENGQWSDFVNTEWCYENV
                     LTGTDKVYWYLNGEGFYEYTYETRMTERVADVAGEGFYSVSYNDEYIYACQYFETEDS
                     VTSPMFYIFDRQYKLIDSVKIDIEEKCCNPLYLTELDDYIILSTSTWNRPPDYYLLKS
                     EIGSGDIKVRKIGE"
BASE COUNT          673 a          537 c          421 g          819 t
ORIGIN      
        1 aagatttatc tcaatgtaaa agcgggcatc tttgtctttc ctttcataac agaatgtatc
       61 ggcatcatta tcggtcagga taaagtctcc gtatatgagg gtttgattct cttttctgaa
      121 ggatatcagt gacttcatgt aattcagtac ggagaatgtg tcctttatct gacttttagc
      181 attatacctc atatagtcgt tggagcaatc tatccatggt tttacttcgg agaatcctcc
      241 gtaagcggca tcttcccact gcatgggtat tcttgcatgg tctctggttc ctgccgatat
      301 cttcaagatt gcttgctcct ctgataaacc ctgctcaaga tactcgttaa acatgtttat
      361 tgcttccaca tcgcggaaat ccgatatatc cttaaatacc gaatcaatca ttgctatttc
      421 ctgaccctga tagaaatacg gggttcctct caatgtgagc ataagtgtga ttatgagttt
      481 tgctgccaaa tcacgtttag attcatcttt tgttatcctg gatacaaatc tggggttatc
      541 atgattttca aaaaccagtg ccggccagca gccgtctttg aaatctcttt gccacttgac
      601 aagaaggggt ttaagttttc taaggtcata tctgtaggaa tcgtgtctgg atttgccggg
      661 gttattgaaa tggtcgaagt tgaatatctg atctaattct ttacgcgact cttccgtcat
      721 gagcttggcg gcttccagtc ctatcccggc accttctccc acggtaaatg cgttgtgctt
      781 gtcaaaagta tttttccgca tttccctaag atactcatgc agtttcggtc cgtaaaacat
      841 atgctcatac cctattgccc ctaccagccc tttcaatgcc ggtaaaccgt cgggtaatcc
      901 cggtgttttg gatataaggt tgattacatc cagccggaat ccgtccactc ctttttccag
      961 ccaccaattt atcatatcgt aaatatcttt acgcatattt tcattttccc agttgaggtc
     1021 catctgtttt ttggaaaaaa catgcaaagc atattcatcc aagtcagggt agtaattcca
     1081 ggcggaaccc ttgaaagcgg aagtccagtt attgggaggt ccgttattct tgccttttct
     1141 gaatatatag tagtcgtgat attttgaatt cttatctttt aacgcttcct taaaccactc
     1201 atgctcgtcc gatgtatgat ttataaccag gtccataatg agtttcatac ctcttttatg
     1261 cacttctctt aaaagaaggt caaaatcggc catatcgccg aattccgtca ttatcttttt
     1321 atagtccctt atatcatacc cgttgtcatc gttgggagaa tcgtaaacgg gagataacca
     1381 tatggcatca atgcctatgt atttaagata atccagtttg gatactattc cgtttaagtc
     1441 tcctattccg tcattattcg aatccttaaa ggacctcgga tatatctgat aaaacacggc
     1501 acttttccac catgtctttt ttaactccga tgtaggactt ttatgcaaat caggttcacg
     1561 gttaagcata tcgataagag aatccacaaa atcttcggtt atctgattct tggtaaggtg
     1621 aggtaatctg cccaccctta tattccccac aataggattt ttaaacagaa atgctccttt
     1681 accggacata tatgcaatca agtccaaaac atccttgcct aaaggggcat taaacagtgt
     1741 ctttatcctg ctgtttcttg tatacatttg cattctccct tctttttaat tttaacacta
     1801 tgaccgaatg taagcaaacg gatattacac ctaatcgtaa gattgtcaga taataatcta
     1861 tattcaaaat acatttgagt ttaagcggaa cctttaacct gaatgaacat acaggtaaga
     1921 acaatcattc ccctattttt ctcactttta tatccccgct gcctatctcg gattttaata
     1981 aataataatc gggcggtctg ttccatgtag atgttgacaa aattatataa tcatccaact
     2041 ctgtaagata tagcgggttg cagcatttct cttcaatatc aatcttaaca ctgtctatca
     2101 gcttgtattg tctgtcaaag atgtaaaaca taggtgaagt aacggaatcc tccgtttcaa
     2161 agtactgaca tgcgtatatg tactcgtcat tatacgagac agaataaaaa ccttcacctg
     2221 caacatccgc aacacgctcg gtcattcttg tttcataggt atattcatag aaaccttcac
     2281 cgttcagata ccagtacact ttatcggtac cggttaatac attttcataa caccattcgg
     2341 tatttacaaa atcactccat tgtccgtttt ccattgaata tctgcatacg gaatatttct
     2401 tatccggaga ataagtatat atgtataaat actcatcctg cggataaaac
//
LOCUS       DAOPWC010000235         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45288_length_2449_cov_2.838764, whole genome shotgun sequence.
ACCESSION   DAOPWC010000235 DAOPWC010000000
VERSION     DAOPWC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45288_length_2449_cov_2.838764"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /gene="rplI"
                     /locus_tag="PKJ65_05485"
     CDS             <1..305
                     /gene="rplI"
                     /locus_tag="PKJ65_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ41318.1"
                     /translation="RKQSESSKKDRELVEANLLQSRLEGKQVKIEVRAGESGKLFGSI
                     TAKDIADAIAEQYKADIDKRKIQLDEPIKNTGDADVVIKIHPEVSINIRLVITEKV"
     gene            307..1638
                     /gene="dnaB"
                     /locus_tag="PKJ65_05490"
     CDS             307..1638
                     /gene="dnaB"
                     /locus_tag="PKJ65_05490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ41319.1"
                     /translation="MSKNVKRVPPHSQEAEQYVLGCLLLDSSAFEPVQVKIDSSMFYI
                     PAHRDIYSALQAVFLSGIPVDIVSVAEQMKKDDTFTDAGGMEYLNKLASQVVSASSAE
                     YYASIIFDKYILRSLISLSGQVFEDAYSDSKEAKELLEYSQQELFNLSEQADTKGVKK
                     ISDLVVRRLDYFRQVSKTKEIPNCIETGFVKLDELILGFRPGDFILIAARPSMGKSTL
                     AMNMAQNMAIRKGKNVLFLSLEMSNEQLTDRMIASEARVPNSTLQRGEADLNEWSKVA
                     ATAGIIHDARIFLDDTSSITVADIRSKCRRFKAAENLDIIFIDYLQLIGSAVKKENRQ
                     QEVTEISRQLKILAKDLKIPVVAMSQLSRASEQRADRVPQLSDLRDSGAIEQDADMVM
                     FIHRPQELDKNATTGIVNLIVAKNRSGSTGNVQLVWQGPIVRFDSYTKRDD"
     gene            1646..>2449
                     /gene="tilS"
                     /locus_tag="PKJ65_05495"
     CDS             1646..>2449
                     /gene="tilS"
                     /locus_tag="PKJ65_05495"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNZ41320.1"
                     /translation="MDDNLIKDKPWYVAFSGGPDSACLLYLLIKKREEIEKKYQVSVK
                     LSAIHVNHNLRQSESKRDQEFCRRLCEKYDIEFLPYDVDVNNYAKEEKLTVEQAARVL
                     RYEIFDDYSYGCVFLGHNKDDNAETVFLNILRGSGVQGLCGMQKVSDHYFRPLLDYTK
                     KEILEFNRDNGIEYVIDSTNQASYYMRNYIRNEIIPALNEKTNKDIVGNLIALADIAT
                     QTQSYINKQIDAEWDKRVTAGKDHVLVDVEDFADMDQVISSGLLRKAIRY"
BASE COUNT          778 a          451 c          608 g          612 t
ORIGIN      
        1 acagaaagca gtcggagagt tcaaaaaaag acagagagct tgttgaggct aatctgttgc
       61 agtccagatt ggaaggtaaa caggtaaaaa tagaggtcag agcgggcgag agcggaaaac
      121 tgttcggctc cataacggca aaagacattg cggatgcgat agctgaacag tataaggcgg
      181 atatcgataa aagaaagata cagcttgatg aacctataaa gaatacaggg gatgcggatg
      241 tagttattaa gatacatccc gaggtaagta taaatatccg cctggttatc acggagaaag
      301 tataatatga gtaaaaatgt caagagagtg cctcctcaca gtcaggaagc cgaacaatat
      361 gttttaggat gtctgttgtt ggacagttca gcatttgaac cggttcaggt taagattgat
      421 tcttccatgt tctatatccc tgcgcatagg gatatatata gtgcgttgca ggccgttttc
      481 ttaagcggta ttcccgtgga tatagtgtcc gtggcggaac agatgaaaaa agacgataca
      541 tttaccgatg ccggcggcat ggaatatctc aacaaacttg catcacaggt ggtgtccgct
      601 tcctcggcgg agtattacgc atccattatc tttgacaaat acatattgcg ttcgctcata
      661 tctctcagcg gtcaggtgtt tgaggatgca tacagcgatt caaaggaagc aaaggaactt
      721 ctggaataca gtcagcagga actattcaat ctttccgaac aggccgatac aaaaggcgta
      781 aaaaagataa gcgatctggt tgtgcgcagg ctggactatt tccgtcaggt aagtaagaca
      841 aaagaaattc ccaactgtat cgaaacgggg tttgtcaagc tggatgaatt gatattggga
      901 ttccgtcccg gggattttat actcatcgcc gcaagacctt ccatgggaaa gtccacattg
      961 gctatgaaca tggctcagaa catggcaata agaaaaggaa agaatgtgct gttcttaagt
     1021 ctggaaatgt ccaatgagca gttgaccgac aggatgattg cttcggaagc cagagttccc
     1081 aattccacac ttcaacgcgg tgaagccgat ttgaacgaat ggtcgaaggt tgccgctaca
     1141 gcggggataa tacatgatgc caggatattt cttgatgata cctcatccat taccgtagcg
     1201 gatatccgat ccaaatgcag acggttcaaa gctgcagaaa atctggatat tattttcatc
     1261 gactatctgc aacttatcgg tagtgcggta aagaaggaaa acaggcagca ggaagttaca
     1321 gaaatatcca ggcaacttaa gatattggca aaagatttga agattccggt tgttgcgatg
     1381 tcccagcttt ccagggcatc cgagcagaga gccgatcgtg ttccgcagtt gagcgatctt
     1441 cgtgattccg gagcaattga gcaggacgcc gatatggtca tgttcattca cagaccgcag
     1501 gaattggaca agaatgcaac tacgggtatt gttaatctca tcgtagcgaa aaacagaagc
     1561 ggttccaccg ggaatgttca actggtctgg caaggaccga tagtcagatt tgacagttat
     1621 accaaaagag atgattaagc tcgatttgga cgataattta attaaagata aaccctggta
     1681 tgtcgccttt tccgggggac ccgattccgc atgtctttta tatcttctga taaagaaaag
     1741 agaagagatt gagaaaaaat accaagtttc agttaaactt tcagccattc atgtaaatca
     1801 taatttacga caatcggaga gcaagagaga ccaggagttt tgcaggcggt tatgtgagaa
     1861 atatgacata gagtttctgc cttatgatgt ggatgtcaat aactatgcta aagaagaaaa
     1921 actgacagta gagcaggctg ccagggtgtt gcgatacgag atattcgatg attattccta
     1981 cgggtgtgta tttttaggcc ataacaaaga cgacaatgcc gaaacggttt tcttgaatat
     2041 attaagagga agcggtgtac agggactgtg cggcatgcag aaagtgtccg atcattactt
     2101 cagaccgctc ttggactata ccaaaaagga aatactggag ttcaaccgtg ataacggtat
     2161 tgaatatgtg atagacagca caaaccaagc cagttactat atgcggaatt atataagaaa
     2221 tgagattatt cccgcactca atgagaagac aaataaggat attgtaggta atcttattgc
     2281 attggccgac attgcaacgc agacgcaatc atatatcaat aaacagattg atgccgaatg
     2341 ggataagaga gtaacggcgg gaaaagacca tgttctggtg gatgttgaag attttgcaga
     2401 tatggatcag gtcatatcct cggggctttt aagaaaagcc atacggtat
//
LOCUS       DAOPWC010000236         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45345_length_2447_cov_3.839465, whole genome shotgun sequence.
ACCESSION   DAOPWC010000236 DAOPWC010000000
VERSION     DAOPWC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45345_length_2447_cov_3.839465"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKJ65_05500"
     CDS             complement(<1..402)
                     /locus_tag="PKJ65_05500"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNZ41321.1"
                     /translation="MIKEELANILNNVYGSSEESIRYFQAPGRVNLIGEHIDYNGGYV
                     FPAAIDKVSTVAVRKRKDDIIKLYATDLRYVVEADMNSLEQYKDLKWGNYQLGVIDQI
                     LKKGIKVKGAEFIFDDKMPLGSDLSSSTAIEI"
     gene            complement(406..1395)
                     /gene="galT"
                     /locus_tag="PKJ65_05505"
     CDS             complement(406..1395)
                     /gene="galT"
                     /locus_tag="PKJ65_05505"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNZ41322.1"
                     /translation="MAELRWHPLIKDWVMVASHRQNRPQMPKNWCPFCKGSEKVPKDY
                     IVMKYDNDFPALSQNPPIPDNLATDLYKTAPLYGKCEVILYSSDHYATLPELDNLHLN
                     ELVKLWKQRFIEISSDKLIKYVFIFENRGEAVGVTMPHPHGQIYGYPFIPKKLELELQ
                     SCKEHTDNTGRCLICDMNREEVSFSDRVIFQNDSFIVYLPFFTEYPYGIYISSKRHIQ
                     YITQFTEKESSDFAKTLKIAAGTLDSLFGFTFPYMMCMHQAPVNTGEDYDYYHFHVEF
                     FPPMRARDKIKYNASSETGAWAHCNPTSVEEKAEELRAAYRRFVTGKGNTKGE"
     gene            complement(1399..2301)
                     /locus_tag="PKJ65_05510"
     CDS             complement(1399..2301)
                     /locus_tag="PKJ65_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNZ41323.1"
                     /translation="MDKKGNKLGEWINNELPEIASQLEALNDDYYLRENTISLAGKER
                     VKNILFLLRSALFPGVYEKCSISKANVDMLIGNNLREAAIELREILESVFSYKCDKDC
                     DNCPINLDEIIITFLDKMPKIRRKLQTDIHAAYVGDPAAQSFEEILLSYPSLEMMSIH
                     RIAHELYKLKVPLIPRMMNEYGHRVTGIDIHPGASIGEHFFMDHGTGIVIGETTEIGN
                     NVKIYQGVTLGAKSFKLDANGNPVKGIKRHPKIEDNVVIYSHATILGGDTVIGHDSVI
                     GASVWLMESVAPYSKIYYNLGSDK"
BASE COUNT          677 a          560 c          415 g          795 t
ORIGIN      
        1 aatctcaatc gcggtagatg aggataaatc gcttcctaaa ggcattttgt cgtcaaatat
       61 gaactccgca ccttttacct taatgccttt ctttaaaatc tgatcaatga ctccgagctg
      121 ataattgccc catttcaagt ctttatactg ttcaagacta ttcatgtccg cttccactac
      181 ataccgtaaa tcggtagcat acagttttat tatgtcatct ttcctttttc ttacagcaac
      241 agtggatact ttatctattg ccgcgggaaa aacatatcct ccgttgtaat cgatatgttc
      301 ccctattaaa ttaactcttc cgggtgcctg aaagtatctg atactctcct cggaactgcc
      361 gtacacatta ttcaaaatat ttgccaactc ttcttttatc ataaactatt caccttttgt
      421 atttcctttt ccagttacaa atcttctata tgccgctctc aactcttccg ctttctcctc
      481 cacggaagtg ggattgcagt gtgcccatgc tccggtctcg ctggatgcat tgtactttat
      541 tttatctctc gctctcatcg gagggaaaaa ctccacatga aaatgataat agtcatagtc
      601 ctcacccgta tttaccggtg cctgatgcat acacatcata tacgggaatg taaatccgaa
      661 aagactgtcc aacgtacctg cagcaatctt taaagtctta gcaaagtccg atgattcctt
      721 ttcggtaaat tgagtaatat attgtatatg tcttttggaa gaaatatata taccgtacgg
      781 atattcggta aaaaacggaa gataaactat gaaactgtcg ttctggaata taactctatc
      841 ggaaaaactg acctcttctc tgttcatgtc gcatatgaga catctgccgg tattatcggt
      901 atgttctttg caagattgca attccagctc caacttcttg ggtataaaag gataaccgta
      961 aatctgcccg tgaggatgcg gcatggtaac tcctacagcc tctcccctgt tttcgaatat
     1021 gaaaacatac tttatcaact tatcggaaga tatctcgata aaccgctgct tccaaagttt
     1081 taccagttca ttcagatgca aattgtccaa ttccggtaat gtcgcatagt gatcggaaga
     1141 atatagtata acttcacatt taccgtacaa cggtgcggtt ttatacaaat ccgttgccag
     1201 attatcggga atcggaggat tttgagacag tgcgggaaaa tcattgtcat atttcatgac
     1261 aatatagtct ttcggtacct tttcactgcc tttacagaaa ggacaccagt ttttgggcat
     1321 ctgcggtctg ttttgtctgt gcgatgccac cattacccag tcttttatca aaggatgcca
     1381 tcttaattca gccataatct atttgtcact ccctaagtta tagtaaattt tggaatacgg
     1441 agcaaccgac tccatcagcc ataccgatgc gcctattacc gaatcatgtc ctattaccgt
     1501 atccccgccc aatattgttg catgagagta tataaccaca ttgtcttcta ttttagggtg
     1561 ccgctttata cctttaacgg gattgccgtt tgcgtccaac ttaaaacttt ttgcacctaa
     1621 agtaacaccc tgatatatct ttacattatt gcctatttcc gtagtctctc ctattactat
     1681 gccggtaccg tggtccatga agaaatgctc tcctatactg gctcccgggt gaatatctat
     1741 gccggtaact ctgtgcccgt actcgttcat cattctggga attaaaggca ctttgagttt
     1801 atataactcg tgagctattc tgtgaatgct catcatctcc agtgacggat agcttaataa
     1861 tatctcctca aaactctgag ctgcgggatc acccacatat gcggcatgaa tatcggtctg
     1921 caactttctt cgtatttttg gcattttatc caggaaggta ataataattt catcaagatt
     1981 aatcgggcaa ttgtcgcagt ccttgtcaca cttgtaactg aatacactct ctaaaatctc
     2041 tcttaactca attgcggctt cccttaagtt gttgcctata agcatatcca cattggcttt
     2101 gcttatggag catttttcat acaccccggg aaacagagcc gagcgtaaaa gaaacagtat
     2161 attctttacc cgctctttcc ccgccagaga tattgtgttt tcacgtaaat aatagtcgtc
     2221 attgagtgct tccagttgtg atgctatttc cggaagctca ttattaatcc attcaccaag
     2281 tttgttgcct tttttgtcca tttctcctcc gctaatcagc aacatacatt atacaacaat
     2341 tagaagataa ataattaaca aaagaagaaa taatacaccg attcattact tcgcttgagt
     2401 gcaaagttaa attccagtat aaaagaaact ctcaaaaaaa tggattt
//
LOCUS       DAOPWC010000237         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45354_length_2447_cov_3.162625, whole genome shotgun sequence.
ACCESSION   DAOPWC010000237 DAOPWC010000000
VERSION     DAOPWC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45354_length_2447_cov_3.162625"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKJ65_05515"
     CDS             <1..525
                     /locus_tag="PKJ65_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41324.1"
                     /translation="TLSNDRVMLKTDESGLPTVISSVTVTGNKVYVDTATWAIKFHSY
                     YTENDIVTVKFWKESKEVKSADIVVLAAESKVSRIEYDANQSFVISSREFYTEPLRIY
                     DQYDIEANLPSNTRWLCNSNFVDSITFDPVAKRLKIVVRGTVTTETEAIISYIAPDGS
                     FAFRGFYTIMPGTY"
     gene            573..1259
                     /locus_tag="PKJ65_05520"
     CDS             573..1259
                     /locus_tag="PKJ65_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ41325.1"
                     /translation="MGHKILVVDDEKNIVDILKYNLEKEGYDVICAYDGKEAIFQALK
                     HKPDLILLDIMLPEYDGFVVCRKLRESTTVPIIMLSAKSEELDKVIGLEMGADDYMTK
                     PFSARELIARVKSNLRRVSYANVDNSELLSFKIRDLEVDEGKCEVLKNGQVLELTARE
                     FELLVFFMRNPEKVFSREFLLDKIWDYEYYGEMRTVDVTVSRLREKLSGDPNEYIVTK
                     RGMGYYFKKD"
     gene            1318..>2447
                     /locus_tag="PKJ65_05525"
     CDS             1318..>2447
                     /locus_tag="PKJ65_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNZ41326.1"
                     /translation="MIIPVGVLMNNNVEQYHYTQFTSNIENGRKMLNYTQGEPLNIYT
                     VYTDMRDLYASSFGIYGDNRSFTIVDRNTNEIYSNDSVFLSHDSSLFINELFLSYNFL
                     SSMAGTPLNGKALQNIKGRTFYDYAITLENLVIYFRYYKTDWQAMVMQFNETLLTALL
                     FSIAVAFVVGYLLSRAISRPIKDMTVRTKAIAEGDFGRTLDVKADDEIGQLSTSINNM
                     SYSLKNMLDEISSEKNKMEIILDNIEGGVIAFNLDGQMIHNNPAAYTLLNRPDMDFTL
                     ESFVKEYNLDIDVSDIPADFYKEDILPVDEEMLAFSIASFKDSLGNAEGVILLFRNIT
                     EQYRLDRMRKEFVANVSHELKTPLTSIKSFTEALMEGVEDPAT"
BASE COUNT          790 a          417 c          558 g          682 t
ORIGIN      
        1 acattgtcca acgatagggt aatgcttaaa acagatgaat cgggacttcc taccgtaata
       61 tcatcggtaa ccgttaccgg gaacaaagtt tatgttgata ccgcaacatg ggcgataaaa
      121 ttccactcgt attataccga gaatgatatt gtcacggtta aattctggaa agaaagcaag
      181 gaagtcaagt cggcagatat agtggtgttg gcagccgagt ccaaggtcag caggattgaa
      241 tatgatgcca atcagtcatt tgtgatatcc tcaagagaat tctataccga acctttacgg
      301 atatatgacc aatatgatat tgaagccaat ttaccttcca ataccaggtg gttgtgtaac
      361 agcaattttg tggattccat tacatttgat cccgttgcca agaggttgaa gatagttgtc
      421 agagggacgg ttactaccga aaccgaagcc attatatctt acatagctcc cgacggatcc
      481 ttcgctttca gaggattcta tacaataatg ccgggaacat attgattgaa aattatggaa
      541 tttttatata ttattaaaaa aggatatgta ttatgggaca taaaatactg gttgtagacg
      601 acgaaaagaa tatagtggat attttaaagt ataatctgga aaaagaaggt tatgatgtca
      661 tatgcgctta tgacggcaaa gaagccatat ttcaggcatt gaagcataag cccgatctta
      721 ttttattgga tattatgttg ccggaatatg acggatttgt ggtatgcaga aagcttcgtg
      781 aaagcaccac cgttccaatc ataatgctgt cggcaaaaag cgaagaactg gataaggtta
      841 tagggctgga aatgggagcg gacgactata tgacaaagcc tttttccgca agagagctta
      901 tagccagagt gaaaagcaac ttacgcagag tcagttacgc aaatgtggat aattccgaat
      961 tattatcctt caagataaga gatttggaag ttgatgaagg aaaatgcgaa gtattaaaga
     1021 acggtcaggt tttggagctt accgcacgtg agtttgagct gctggtcttc ttcatgagaa
     1081 accctgaaaa agtattcagc agggagttct tactggacaa gatatgggat tatgagtatt
     1141 acggagagat gaggacggta gatgtcacgg tcagcaggtt aagagaaaaa ctttcgggtg
     1201 atcctaacga gtacatagta acgaaaagag gaatgggcta ttatttcaaa aaagactgat
     1261 aagcatatat ccttacagac caaaatggtc ttctacatct gtatcatagc tgtatgtatg
     1321 ataatacctg tcggtgtgct catgaataat aatgtggagc agtatcatta cacgcagttt
     1381 acaagcaata tagagaacgg caggaaaatg ctcaattata cgcaagggga acctcttaac
     1441 atatatacgg tatacaccga tatgagagac ctttatgctt cttcattcgg tatatacgga
     1501 gataaccgca gttttaccat agtggacaga aataccaatg aaatatacag taacgattcg
     1561 gtttttttat cgcacgacag ctctttattt ataaatgagc tgtttttgtc atataatttc
     1621 ctgtcttcca tggcgggtac gccgttaaac ggtaaggcgt tgcagaatat aaagggaaga
     1681 acattctatg attatgccat aacactggaa aacttggtta tatatttccg ctattacaaa
     1741 accgattggc aggctatggt tatgcagttt aacgaaacgc tgcttacggc gttgctgttt
     1801 tccatagcgg tggcatttgt tgttggttat cttttatcca gagccataag cagacccatt
     1861 aaagatatga ccgttcgtac aaaggcaatt gccgaagggg atttcggaag aacattggat
     1921 gtaaaggcgg atgacgaaat aggacagctt tccacaagta taaataacat gagttacagc
     1981 cttaagaata tgttggatga aatatcttcg gaaaagaaca agatggagat aatattggac
     2041 aatatcgaag gcggagttat agctttcaac cttgacggtc agatgataca taacaatccg
     2101 gctgcatata cgctgctcaa cagaccggat atggatttca cgctggagag cttcgtgaaa
     2161 gaatataatc tggatattga tgtgtcggat attcccgccg atttttataa agaagacata
     2221 cttcccgttg atgaagagat gcttgcattc agtatagctt cttttaagga cagtctgggc
     2281 aatgccgaag gtgtcatact gcttttcaga aatataaccg agcagtacag attggaccgt
     2341 atgagaaaag agtttgttgc caatgtgtcg catgaactta aaacccctct tacttcgata
     2401 aagagtttta ccgaagcttt aatggaaggt gtggaagatc ccgctac
//
LOCUS       DAOPWC010000238         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45430_length_2444_cov_3.594391, whole genome shotgun sequence.
ACCESSION   DAOPWC010000238 DAOPWC010000000
VERSION     DAOPWC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45430_length_2444_cov_3.594391"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKJ65_05530"
     CDS             <1..295
                     /locus_tag="PKJ65_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41327.1"
                     /translation="THYTLTSVILNIYIEKNLETDVTVIKGYDYVPTYMLTPEWGGSR
                     PYVLPVHDAIKEYENGSSTLINEKIYKDLLASLKICHDIIGRDYDYYYIKRTD"
     gene            complement(297..2120)
                     /gene="typA"
                     /locus_tag="PKJ65_05535"
     CDS             complement(297..2120)
                     /gene="typA"
                     /locus_tag="PKJ65_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNZ41328.1"
                     /translation="MIREDLRNIAIIAHVDHGKTTLVDAMLKQAGVFRKNQVVADRVM
                     DSGDLEREKGITILAKNASVRYKNTKINIVDTPGHADFSGEVERVLKMVDGVLLLVDA
                     FEGPMPQTRFVLSKALELNHKVIIVINKVDREGADIRKTENDVLELLMELQANDTQLD
                     SPYVYCSAKEGAASLNPEIKGTDLSPLFDTVLQHINPPGGDENKPLQVLISSIDYNDY
                     VGRIAIGRIERGHIKENSDVAVVNYHNPEEPHRAKVTNIYSFEGVKREPVEEAYVGDI
                     ISFSGIPKISIGDTICNPEFVEAIEFAKISPPTIEMTFSVNDSPFAGREGKYVTSRHL
                     RERLFRETLKDVSLKVKETESADAFIVMGRGEMHLSILVETMKREGYEFQLSTPRVLF
                     TDIDGVKQEPMEHVYIDVPSDCVGAVCEKVGKRKGEMLCMNLSGTRMKMEFSVPARGL
                     FGYRNEFLTDTAGEGIMNTLYEGYAPYKGEIGYRSNGSLIAFETGTAVTYGLYNAQER
                     GVLFINPGEEVYAGMVVGETPKDEDISVNVCKKKHVTNVRAAGSDEALRLSPPKKMSL
                     EQAMEYIKEDELLEITPLSMRIRKKILDPVMRMRSKFKDKQ"
     gene            complement(2256..>2444)
                     /locus_tag="PKJ65_05540"
     CDS             complement(2256..>2444)
                     /locus_tag="PKJ65_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41329.1"
                     /translation="FLRKYNGPVAKNVYIINGLHNGQFIIKDNTLNEVRVGYTTPILK
                     TADLESVIADIKNTEYKE"
BASE COUNT          645 a          596 c          393 g          810 t
ORIGIN      
        1 aacccattac acgctgactt cggtaatatt gaatatctat atagaaaaaa atcttgaaac
       61 cgatgtgacc gtaatcaaag gatatgatta tgtgcccaca tatatgctta ctccggaatg
      121 gggaggctcc agaccatatg tgcttcccgt tcatgatgcc ataaaagaat atgaaaacgg
      181 ttcaagtaca ttgataaatg aaaaaattta caaggattta ttggcatctt tgaagatttg
      241 tcatgacatt atcggtcgcg attacgatta ttactatata aaaagaacag attaatctat
      301 tgtttatctt taaatttcga tctcatacgc attacgggat ccaatatctt ttttcttatg
      361 cgcatactta agggggttat ttccagtaat tcatcttctt taatatattc cattgcctgc
      421 tccaggctca tcttcttcgg aggagataag cgcagagcct catcgcttcc cgccgccctc
      481 acatttgtaa catgcttctt tttgcataca ttgaccgaga tgtcctcatc cttgggtgtc
      541 tcgcctacaa ccattccggc atacacttcc tctccgggat ttataaaaag cactcctctt
      601 tcctgagcat tatataagcc gtatgtaacc gctgttcccg tttcaaacgc aatcaacgaa
      661 ccgttgcttc tgtaccctat ttctccctta tacggagcat atccttcata gagggtattc
      721 attatacctt ctcccgcagt atcggtcaga aactcgttcc tgtagccgaa caagcctctc
      781 gcaggaacag aaaactccat tttcattctg gtaccggaca gattcatgca cagcatctcg
      841 ccttttcttt ttcccacttt ctcacatacc gcccctacgc agtcggaagg tacgtcaata
      901 tatacatgct ccatgggttc ctgttttact ccgtctatat ccgtaaacag caccctgggc
      961 gtactcaatt gaaattcata tccttccctc ttcatggtct ccaccagaat ggacaggtgc
     1021 atttctcctc ttcccataac aataaaagca tcggcggatt ccgtttcctt cacttttaac
     1081 gaaacatcct tcagtgtttc tctgaacaac cgctctctta aatgcctgga tgttacatat
     1141 ttaccttctc ttccggcaaa cggactgtca ttaaccgaga aggtcatttc tattgtagga
     1201 ggagaaattt ttgcaaattc tattgcttcg acaaattccg gattacatat cgtgtctcct
     1261 atagaaatct ttggtattcc ggaaaaagaa ataatatctc ctacatatgc ttcttcgaca
     1321 ggttctcttt taactccttc aaatgaatat atattggtta cctttgccct gtgaggttct
     1381 tcgggattat gatagttaac tacggcaaca tcgctgtttt ccttaatgtg acctctttct
     1441 attctgccta tggctattct tccgacatag tcattgtagt caatggaaga aatcaacacc
     1501 tgcaacggct tattttcgtc gcctcccgga ggattaatat gttgcaaaac ggtatcaaac
     1561 agcggcgaca aatccgttcc ttttatttcc gggttcagtg aagccgctcc ttcttttgcg
     1621 gaacagtaca cataaggact gtccaattgc gtgtcgtttg cctgcaattc catcagaagt
     1681 tccagcacgt cgttttccgt ctttcggata tccgcacctt ccctgtccac tttatttatc
     1741 acaattatta ctttatgatt aagttccaaa gctttggaaa gaacaaacct tgtctgcggc
     1801 ataggccctt caaaggcatc caccaataac agtactccgt caaccatttt aagtacgcgt
     1861 tccacctctc cggaaaaatc cgcatgtccg ggggtatcca ctatattgat cttggtattc
     1921 ttgtatctta ccgaagcatt cttggcaaga atggttatac ccttctctct ctccagatcg
     1981 ccggaatcca ttactctgtc cgcaacaacc tgatttttcc tgaacactcc cgcctgttta
     2041 agcatggcat ccaccagtgt ggtcttgccg tggtctacat gcgctattat tgctatattc
     2101 cttaagtctt cccttatcat aagccttcct caaaagtttt caacttatta tacacctgta
     2161 agattaaaag aaaagcataa tattttatac ttaacatcaa aggctatgtc tctttaactt
     2221 ttttgagaaa cctattatat tttcctatca tatttctatt ctttatattc cgtatttttt
     2281 atatcggcaa taacactttc caaatccgcg gtttttaaaa taggtgtcgt atatcctact
     2341 ctaacctcat tcaaggtgtt atctttaatt ataaactgac cgttatgtaa accgtttatt
     2401 atgtaaacat tctttgcaac tggtccatta tatttcctca gaaa
//
LOCUS       DAOPWC010000239         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45673_length_2435_cov_2.761345, whole genome shotgun sequence.
ACCESSION   DAOPWC010000239 DAOPWC010000000
VERSION     DAOPWC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45673_length_2435_cov_2.761345"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /gene="obgE"
                     /locus_tag="PKJ65_05545"
     CDS             <1..1050
                     /gene="obgE"
                     /locus_tag="PKJ65_05545"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ41330.1"
                     /translation="GKSGENTVIRVPVGTLITDTDRNKLICDLNKDQMSFTVCEGGRG
                     GRGNTRFATPTRQAPEFSEAGMEGEEKNLRLELKLLADVGIIGMPSVGKSTLISVITS
                     ARPKIADYHFTTLVPNLGVVQLNDATQFVVADIPGLIEGASEGAGLGHAFLRHIERTK
                     LLVHMIDASELEERDAVKDFETINNELAKYNPRLYERHQICVMNKTDLCTTDEQIKRA
                     EEIEAAIKEKGYEVLKISAATNKGTDILVKRCAELLSKLPDTVIMPEAEEYAVYEHEE
                     DERFTVRKEGNIYYVEGAWATKLVNSINIYNTESLRYFQRQLREKNVIEELEKAGIQE
                     EDLVVIDDFEFEYFR"
     gene            1062..1355
                     /gene="yhbY"
                     /locus_tag="PKJ65_05550"
     CDS             1062..1355
                     /gene="yhbY"
                     /locus_tag="PKJ65_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNZ41331.1"
                     /translation="MITSKQRAYLKGLAQKIQPIIQLGKDGISENTLIMYEEALEARE
                     LVKTTVLKSCEYEPLQVANDIAEAIGAEVVQVIGNKFVLYKQSREKKCINLDL"
     gene            1546..2151
                     /locus_tag="PKJ65_05555"
     CDS             1546..2151
                     /locus_tag="PKJ65_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage infection protein"
                     /protein_id="HNZ41332.1"
                     /translation="MSSKEIVTYAIEKNNGIAKVSDMVNDGASRQDIKKLYADGYIQR
                     VSQGYYQLTSNTYLPDEQIIASTIPKSVVCLESALFYYGYSDFTPRKIAITIPRSSSR
                     RAIKATDIPLLVYYVSDEIYDIGKTIVTDNGVTFSIYDRERTICDIFKHRNKIDRETF
                     NKAVNAYAKDEDKDLHKLSEYAKRLRVYKKVTEIMEVLLNG"
     gene            2144..>2435
                     /locus_tag="PKJ65_05560"
     CDS             2144..>2435
                     /locus_tag="PKJ65_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ41333.1"
                     /translation="MGDIAASVLARLKNTAKATGRSNQLCLQLFCQEEFLRRLQKSKY
                     SENFILKGGYYIYAITDFDSRVTFDVDFLAKRIPNTPEHIKVTEYMDELLKFI"
BASE COUNT          883 a          361 c          526 g          665 t
ORIGIN      
        1 ggaaaatccg gagaaaatac cgtcataaga gttcccgtag gcacccttat aaccgatacc
       61 gacaggaata agcttatatg cgatttaaac aaagatcaga tgtcttttac agtatgtgaa
      121 ggcggaagag ggggaagggg aaatacacgt tttgccacac ctacaagaca ggctcccgag
      181 ttttccgaag ccggcatgga aggggaagag aaaaaccttc gtctggaatt aaagctttta
      241 gcagatgtgg gtataatcgg tatgccttcg gtaggtaaat ccaccttaat ctcggttata
      301 acgtccgcaa gacccaagat tgccgattat catttcacca cactggtacc caatctcgga
      361 gtagtgcaat taaatgatgc aacacagttt gttgttgccg atattcccgg tttaattgaa
      421 ggagcatcgg aaggagcagg cttgggacat gcatttttgc gacatatcga gagaacaaaa
      481 ttattggttc atatgataga tgcatcggaa cttgaagagc gagatgcggt aaaggatttt
      541 gaaactatca acaatgagct ggctaagtat aatccgcggc tctatgagcg tcatcagata
      601 tgtgtgatga ataagaccga tttatgtaca accgatgaac agataaagag agcggaagaa
      661 attgaagccg caattaaaga aaaaggctat gaagtattga aaatttccgc cgccacaaat
      721 aaaggaaccg atatattggt aaaaagatgt gcggaattac tcagtaaact gcccgatacg
      781 gttatcatgc cggaagcgga ggaatatgcg gtatatgaac atgaagaaga tgagcgtttc
      841 accgttcgta aagaagggaa catatactat gtagaaggcg cttgggcaac caagcttgta
      901 aactccatca atatttacaa taccgaatcc ttgagatatt tccaaagaca attaagagaa
      961 aagaatgtaa ttgaagaatt ggaaaaggcg ggtatacagg aagaagattt agtggtaata
     1021 gatgattttg aattcgaata tttcagataa ggggtatata tatgataaca agtaagcaga
     1081 gagcatattt aaaaggactg gcacagaaaa tacaaccgat tatacaactg ggcaaagacg
     1141 gaataagcga aaacactctt attatgtatg aagaggcttt agaggctcgt gaacttgtaa
     1201 agacaactgt gctgaaaagc tgtgagtatg agcctttgca ggttgcaaac gatatagccg
     1261 aggcaatagg tgccgaagtt gtgcaggtaa taggtaataa gttcgtattg tataagcaat
     1321 caagagaaaa gaagtgtatt aatctggatt tataaagacc attttaagaa agtacatctt
     1381 tgtatctgga ataatactta ataaatatgg taaatacagt aattaagaga agaattaaag
     1441 tttcttcttt ttttatttga tattagataa aaatatgtta tactactaaa agtaacacac
     1501 atagcatata agtgtgtatc aaatagaaat tggagggttg ctatattgag ttcaaaagag
     1561 atagtaacat atgccataga aaagaataac ggaattgcaa aagtatcgga tatggttaat
     1621 gacggtgcat caagacagga tattaaaaag ttgtatgctg acggatatat tcaaagagta
     1681 agtcagggat attatcagtt gacttctaat acttatttgc ctgatgagca aattattgct
     1741 tcaactatac caaaatcggt tgtttgtctt gaatcggcat tgttctacta cggatatagc
     1801 gattttactc caagaaaaat agcgataact attcctcgtt catcatcaag aagagcaata
     1861 aaagctacgg atattccctt gctggtgtat tatgtttcag atgagatata tgatattggc
     1921 aaaacaatag ttactgataa cggagtaacc ttttctattt atgatcgtga aagaactatt
     1981 tgtgatatat tcaagcatag aaataaaata gacagagaaa cttttaataa agcagtcaat
     2041 gcctatgcaa aggacgaaga taaagatttg cataaacttt ctgaatatgc aaaaagattg
     2101 cgtgtttata aaaaggttac tgaaataatg gaggtattgc tgaatgggtg atatcgctgc
     2161 ttcagtgctt gcaagactaa aaaatacagc aaaagcaaca gggcgtagta atcagctctg
     2221 tttgcaactt ttctgtcaag aagaattttt gcgtagatta caaaaatcaa aatattcaga
     2281 aaattttatc ctaaaaggag gttattatat atatgcaatt acggactttg acagtagagt
     2341 aacatttgat gttgacttcc tcgctaaacg aataccgaac acacctgaac atataaaagt
     2401 tacagagtac atggatgagt tattgaaatt tatta
//
LOCUS       DAOPWC010000240         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45793_length_2430_cov_2.412211, whole genome shotgun sequence.
ACCESSION   DAOPWC010000240 DAOPWC010000000
VERSION     DAOPWC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45793_length_2430_cov_2.412211"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..2103)
                     /locus_tag="PKJ65_05565"
     CDS             complement(7..2103)
                     /locus_tag="PKJ65_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ41334.1"
                     /translation="MDRITAILKNRYFILALLMLLIMAVFIGKLYKIQIIDGKLYISQ
                     AAARIERGYDIQAARGNIYDRNGVLLAYNRECQNVYLTKAFTASANLNPTLLCLYDLL
                     TQNNESFLKSLRSALEYNQEYETFVFNENWTKQDLLAWQTRPDLFARTESAVQYDANE
                     FFKYLRKYFYVDSSYSNLDAYKIVTMRYEILKMRPDYISGYVHIASDVSLDTVAYITE
                     NSHKLGGVSLRKSYVRAYTEDAAKVAHIIGYVGLVSEQELKADETLSPDYMIGKMGVE
                     QYCEKYLRGRNGYAIVETDVSGKVISVVEGKRPVNGNDVYLTIDIELQKAALNALEVT
                     IEEIIAKADGEKNFGDANAAVAIVIDVDTGELLVCANNKYFDPSWFVDNDEQSVAKRM
                     ETITDAVNTPMFNRAIQAMYTPGSIFKPIVAVAGLESKTIAHDSVIECKMKAEIGEHN
                     FTCLSWHGKINVTKAIEQSCNIFFNQLGVDIGIDAIDAWARAFGFGEKTGIDLPNESS
                     GIISGKEYKMQTFGERWWPADTAQTAIGQLYNNFTPIQIASYTATLASAGKKYTPHVI
                     LKIVDESGDIVYEGDKSYTDIGMSKETDATVRHAMEQVAKEGTASRIFAKYPLRVAAK
                     TGTAETGWEVDSSSNGLSIAYAPADNPDIAACVVIEKGVWGSYTVPAIMRIFNEYFDI
                     QPEEEIIDDTLVTNEQ"
     gene            complement(2078..>2430)
                     /locus_tag="PKJ65_05570"
     CDS             complement(2078..>2430)
                     /locus_tag="PKJ65_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41335.1"
                     /translation="VYDMLYSRFLGPMTVLFFLRGLVTIVIGRRFKQENVLMLTLAGF
                     AMALVSRVYTIILSVGFKDFFRAFGMFIADIGFSALYTALIIAVVTTIMFLIYNSYGR
                     RSKRLLYGQNNRDT"
BASE COUNT          632 a          583 c          461 g          754 t
ORIGIN      
        1 cctcctctac tgttcatttg taaccaaagt atcatctatt atctcttctt ccggttggat
       61 gtcgaaatat tcattgaata ttctcattat tgccggaacg gtgtaggaac cccacacacc
      121 tttttctatt acaacgcaag cggcaatatc aggattatcg gcaggtgcgt aggctatgga
      181 aagaccgttg gaagaagaat ccacttccca tcccgtttcc gccgttccgg tcttggctgc
      241 aactctaaga ggatatttgg cgaatatcct gcttgcggta ccttctttag caacctgctc
      301 catagcatga cgtacggtgg cgtcggtttc ttttgacatc cctatatctg tataggattt
      361 atccccttcg tatacaatat caccggactc atcaacaatt ttcagtatta catgcggagt
      421 atatttctta cctgccgaag ctaaagttgc cgtgtagctg gctatctgta tcggagtgaa
      481 gttgttatac aactgtccga ttgccgtttg ggcggtatcg gccggccacc acctttcccc
      541 gaaagtctgc atcttgtact ctttaccgct gataattccg ctgctttcat tgggaaggtc
      601 aatacccgtc ttttctccga atccgaatgc ccttgcccac gcatctatag catctattcc
      661 tatatccaca cccagctgat tgaaaaaaat gttacacgat tgctcgattg ctttggttac
      721 atttattttg ccgtgccaac ttaaacatgt aaaattatgt tcccctattt ccgccttcat
      781 tttgcattcg attaccgaat catgggctat ggttttgctc tccaaaccgg caacggcaac
      841 tatgggtttg aaaatggaac cgggggtata cattgcctga atggctctgt tgaacatagg
      901 cgtatttacc gcatcggtta tggtttccat cctttttgcg accgattgct catcattatc
      961 tacaaaccac gaaggatcaa aatatttgtt gtttgcacag accagcagtt caccggtatc
     1021 cacatctata actatggcga ctgcggcatt ggcatctccg aagttctttt cgccgtcggc
     1081 ttttgcgata atttcttcaa tagtgacttc cagagcattc agagcagctt tttgcagctc
     1141 aatatcaatt gtaagatata catcatttcc gttgaccggt cttttgcctt ccaccaccga
     1201 tataactttt cctgaaacat cggtttccac aattgcatat ccgttgcgac cgcgaagata
     1261 tttctcacaa tactgctcca ctcccatctt gcctatcata tagtcgggcg aaagcgtttc
     1321 atcggcttta agttcctgct ccgacaccag ccccacataa cctataatat gcgccacttt
     1381 tgccgcatct tcggtatagg ctcttacata acttttccgt aatgataccc ctccgagttt
     1441 atggctgttc tccgttatat atgccacggt gtccagcgat acatccgatg ctatatgaac
     1501 ataacccgat atatagtccg gtctcatttt gagaatttca tatctcatgg tcactatttt
     1561 gtatgcatca agattggaat aggaactgtc cacataaaaa tacttccgaa gatacttaaa
     1621 gaattcattg gcatcgtatt gtacggccga ttcggtacgg gcaaaaaggt cggggcgtgt
     1681 ctgccaggct aaaagatcct gcttggtcca gttttcattg aatacgaatg tctcgtattc
     1741 ctgattgtat tccagagccg atctcaaact ttttaaaaaa ctttcattgt tttgcgtcaa
     1801 cagatcataa agacaaagaa gcgtgggatt gagattggca ctggctgtaa atgcctttgt
     1861 aaggtagaca ttctgacatt cccggttgta tgcaagcagt acaccgttac ggtcataaat
     1921 attacctctt gccgcctgaa tatcatatcc tctttctata cgggcagcag cctgactgat
     1981 atagagtttt ccgtctataa tctgtatttt atagagttta cctataaaaa ccgccatgat
     2041 aagaagcatc agcagcgcca atatgaaata tcgattttta agtatcgcgg ttattctgtc
     2101 catatagcag cctcttgctc cttcttccgt aggaattgta tatcaagaac attattgtcg
     2161 taacaaccgc tattataagg gcggtatata aggcggaaaa gcctatatcc gctataaaca
     2221 taccgaatgc tctgaagaaa tctttaaatc ccaccgataa tatgattgtg tatactctgg
     2281 ataccagtgc catcgcaaat cctgcaaggg taagcatcag cacattttcc tgtttaaacc
     2341 ttcttcctat gacgatggtt acaagtcctc ttaagaaaaa cagaacggtc atgggaccta
     2401 aaaatctgct gtacagcata tcgtatacaa
//
LOCUS       DAOPWC010000241         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_45876_length_2426_cov_3.787431, whole genome shotgun sequence.
ACCESSION   DAOPWC010000241 DAOPWC010000000
VERSION     DAOPWC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45876_length_2426_cov_3.787431"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKJ65_05575"
     CDS             complement(<1..336)
                     /locus_tag="PKJ65_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HNZ41336.1"
                     /translation="MTIYGAAIIAGCMFVGSFIGDALGGLMGIDANVGGVGFSMLFLI
                     LITGSDLFGKKSSAATSEGLEYWQSMYIPVVAAMTASQNVVQAVSGGVMAVLAGLIAV
                     AVGFALLPVL"
     gene            complement(578..1201)
                     /locus_tag="PKJ65_05580"
     CDS             complement(578..1201)
                     /locus_tag="PKJ65_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41337.1"
                     /translation="MTHTLHRKGDKEGLSNDYIVFAISAQTVNAKGSAPKFKEFGDIV
                     LKYNPVNYGDMKTGNAYNVGVDKVHESYKDNSIVHAVFTEEDIVAKVLKELREADLGL
                     SIVLSGIVEHTSECCHKISIEPHTIEYSLGIHGKTDSLPEDNVVEISTMCGHGMVSFS
                     LIEHFCEEIEKGRITADEVARKLAKQCHCGVFNPTRAAEILRLMTDM"
     gene            complement(1204..1929)
                     /locus_tag="PKJ65_05585"
     CDS             complement(1204..1929)
                     /locus_tag="PKJ65_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ41338.1"
                     /translation="MYINQELCVQCHACIPYCPMKAIEYKEESKRVEINIDECVECEI
                     CGNADVCPTGALTMEEMKWPRILRNQFSNPLITHPDTGVPGRGTEEMKTNDVTGRFKR
                     GMVGIAIEMGRPGTGTRFYDVEKVAMAVAKVGVHFEPHNPVTKLMTDNTTGKMKDDVL
                     NEKALSAIVEFDIPIEKTKTVLEQLKTVTPLINTVFSLDLACRLNEDGSNPAVCLAEE
                     AGYKVSVNGKTNVGLGRPLAKEE"
     gene            complement(2011..>2426)
                     /locus_tag="PKJ65_05590"
     CDS             complement(2011..>2426)
                     /locus_tag="PKJ65_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNZ41339.1"
                     /translation="GNEGTLQVAVENIRTDFGIRIPFVNYKVLCQDVTAEIVGTKRVD
                     HSGEFEVSDAMFLAPEIVREDAICKGDDGNFPLVYTDIYGKYRINYPLNWKQVAPSGA
                     MGEAHKATPEKGKQLNDAALKRAVEFLEQFMEDED"
BASE COUNT          666 a          615 c          379 g          766 t
ORIGIN      
        1 caataccggc agaagtgcaa atcctacagc aactgcaata agccctgcta ggacagccat
       61 aaccccacct gacacagcct gtacaacgtt ttgtgatgca gtcattgccg ctaccaccgg
      121 aatgtacatg ctctgccagt attccagccc ttcggaagtt gctgccgacg attttttccc
      181 aaataaatca gaaccggtaa ttagaattag aaaaagcatg gaaaatccaa cgcctcctac
      241 attggcatct atgcccatca atccccccag agcatcacct ataaagcttc caacgaacat
      301 acatccggca ataattgccg ctccatatat ggtcatagta ttctctcctt caaataatct
      361 tagcaatagc taatttgaac gtacatttca aactcaaagt tcaccaatat aattatatat
      421 aacacattta taactatatt tctcgtcttt tttggactct attttgcaat aggcaaagtt
      481 attatacgcc attctgtttc tttacttatt aaatgtctaa acttaacaat aagggcagaa
      541 gattccttat cttctgccct tactgatagc acaaatctta catatcagtc attaatcgta
      601 gtatctctgc cgccctcgtt ggattaaaca ctccgcagtg acactgctta gccagctttc
      661 ttgccacctc atctgcagta atcctaccct tttctatctc ttcacaaaag tgttcaatca
      721 gcgaaaagga caccattcca tgaccacaca tagtactaat ctcaactaca ttatcttccg
      781 ggagactgtc tgtctttcca tgtataccaa gggaatactc aatagtatga ggttcaatac
      841 taattttgtg acagcattcg gatgtgtgtt caacaattcc cgatagcaca attgaaagtc
      901 ctaagtccgc ttccctaagt tccttaagca ctttagcaac aatgtcttct tctgtgaaca
      961 ccgcatgtac aatagagtta tccttatagc tttcatgtac tttgtcaaca cctacattgt
     1021 acgcatttcc cgtcttcatg tccccgtagt ttaccggatt atattttaac acaatgtctc
     1081 caaactcctt aaatttaggt gcgctgcctt ttgcattaac agtttgagca gagatagcga
     1141 atactatata atcattggat aaaccttctt tatctccttt gcgatgtaaa gtatgtgtca
     1201 tttttattcc tccttagcca aaggtctgcc taaaccgacg tttgttttcc cgtttacaga
     1261 tactttataa cctgcttctt cagccaggca aactgcaggg ttgctgccgt cttcattaag
     1321 acgacaagca aggtcaaggc taaacacagt attaattaaa ggcgttacag ttttcaactg
     1381 ttccaatacc gttttggttt tctcaatcgg aatgtcaaat tcaacaattg cggacaaggc
     1441 cttttcgttt agaacatcgt ccttcatttt tccggtggta ttatccgtca tcagctttgt
     1501 caccggatta tggggttcaa aatgtactcc tacctttgca acagccatag ctactttttc
     1561 cacatcataa aaacgtgttc cggttcccgg gcgtcccatt tcaatagcta ttcccaccat
     1621 acccctttta aacctgcccg taacgtcgtt ggtcttcatt tcttcggtgc cacgtcccgg
     1681 caccccggta tcagggtggg taataagcgg attagaaaac tgatttctta atatacgggg
     1741 ccatttcatc tcttccatgg tcagtgcacc tgtgggacac acgtctgcat taccgcagat
     1801 ttcacattcc acgcactcgt ctatatttat ctctactcgc ttgctttctt ccttgtattc
     1861 aattgctttc attggacaat aaggaataca ggcatggcat tgaacacaaa gttcttggtt
     1921 aatatacatt gatatacctc cttatccgat taatgatggt caatagccaa gccaacatga
     1981 cacggcacgt ttttgccaac tttctcaaca ctaatcttca tcttccataa actgctctaa
     2041 gaactcaaca gcccttttaa gtgcagcatc attaagctgt ttcccttttt ccggagtcgc
     2101 tttatgagcc tcacccattg ctccgcttgg agctacctgt ttccaattca acggataatt
     2161 tattctgtat ttaccatata tatcagtata tactaaaggg aagttcccat cgtctccttt
     2221 acatatggca tcttctctta caatttccgg agccaaaaac atagcatcag atacttcaaa
     2281 ttcacccgaa tggtctactc tttttgttcc cacaatctct gcggtaacat cttgacaaag
     2341 aaccttataa ttaacgaagg gtattcttat accaaaatct gttctgatgt tttcaacggc
     2401 cacctgaaga gtaccttcat tacccc
//
LOCUS       DAOPWC010000242         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_46005_length_2421_cov_5.383770, whole genome shotgun sequence.
ACCESSION   DAOPWC010000242 DAOPWC010000000
VERSION     DAOPWC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46005_length_2421_cov_5.383770"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKJ65_05595"
     CDS             <1..475
                     /locus_tag="PKJ65_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41340.1"
                     /translation="LSGGEQQRVAIAIAMANDPKILLADEPTGAVDTATAALIYNIFR
                     KLNTEYGITIIIVTHDRAISKNVDRVVTIEDGRTGSEYIKKKSYKEQIMELDRMTGVE
                     ESESHEEMIVVDRQGRLKIPEEYLELAKIPNNSKVRIRMQEEKIVIYTEEEKDEE"
     gene            465..1589
                     /locus_tag="PKJ65_05600"
     CDS             465..1589
                     /locus_tag="PKJ65_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41341.1"
                     /translation="MKNRTFIILLIIASVMLSFLTGCTYFYPLEGRYHAVQPELIEQK
                     KVELDSFEVRKTDVYEGFDLGEESGRRLVYVNDPKKTTIVRSEIERAKILKLHVKPDD
                     YVKQGDILVEYVYEVNKDAEFQVNWNKRRAEIRYENAYIQYQQGRINEQTLENLHKSY
                     IVAKNALDSFNAIPDKYTLRAPVDGHIAKIEGDTVSTEEVQTLAFHICNIEDGILLTY
                     AREQNQYAAYPFMLCFEGTGAELYDEQSDRTYSGRVSMSNMKFKKEYVDDLSYFSGGA
                     YLYLVLELTDNKIPEDVSFYQEFTGKLIRREAHDVLIVSRSAVDMEKSDDPEEPFVYQ
                     VYKILPDNTIERVRIKIGDIFDAYYEVIEGLEEGDKVVLR"
     gene            1586..>2421
                     /locus_tag="PKJ65_05605"
     CDS             1586..>2421
                     /locus_tag="PKJ65_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41342.1"
                     /translation="MREKRANLFITIIILLIFTAILTSCATQAVTYEVPVFDIPKEVF
                     TTLKVEKQNIHDYERINLDVQSSGGSRGIVFTYSDFESATLIVSERDAVVQEGDVLAV
                     KSDKALEYDLRLKLEDLELKQLLYEELYRKHLNTGEGYYDMQLALSDMELAQFNYDKT
                     LEKFETLQFKAPYSGTVNNIISTYKNEKDDFNNEYKVYTVTYYITPDNYSVSLQITPA
                     GYPYFEEMGYKSGDTLQIYDKDGKIHNMRISTMFTRTTGAVGSKTKTAFVASLINPNI
                     TT"
BASE COUNT          849 a          390 c          554 g          628 t
ORIGIN      
        1 gctttccgga ggagaacagc aaagagtagc catagccata gcaatggcaa acgaccctaa
       61 aatattgctt gctgatgaac ctaccggagc ggtggatacc gctactgcgg cattgatata
      121 caatattttc cgaaaactca acacggaata cggaattacc atcataatag tcacgcatga
      181 cagagcaata tcaaagaatg tggacagggt agtgacaatt gaagacggaa gaacgggaag
      241 cgaatatatc aagaaaaaat cctataaaga acagataatg gaattagaca gaatgacagg
      301 agtggaagag tcggaaagtc atgaggaaat gattgttgta gacaggcagg gacgcttaaa
      361 aatacccgaa gagtatcttg aacttgcaaa aattcccaac aacagtaaag tgcgtatcag
      421 aatgcaggag gaaaagattg tgatatatac ggaggaggag aaagatgaag aatagaacct
      481 ttattatcct gcttattatt gcctccgtaa tgctttcatt tcttacaggt tgcacatatt
      541 tctatccgtt ggaaggcaga tatcatgccg tacagccgga actgattgag caaaaaaagg
      601 tggaattgga tagctttgaa gtaagaaaaa ccgatgtata tgagggcttt gacttaggtg
      661 aggaatcagg taggcgactg gtatatgtga acgatccgaa aaaaactact atagtgagaa
      721 gtgaaatcga acgggccaag atactaaaac tgcatgtcaa acccgacgat tatgtcaagc
      781 aaggcgatat cttggtagag tatgtatatg aagtaaacaa agatgcggaa tttcaggtca
      841 attggaataa aagaagagcg gaaatacgat atgagaatgc atatatacaa tatcagcagg
      901 ggaggattaa cgagcaaact ttggaaaatc ttcataaatc ctatatagtt gcaaaaaatg
      961 ctctggactc gtttaatgcc ataccggaca aatatacatt gagagcgccg gttgacggcc
     1021 atatagccaa aatagaagga gatacggttt ccactgagga agtgcagaca ttggcttttc
     1081 atatatgtaa tattgaagac ggtatcctgt tgacatacgc ccgagaacag aatcaatatg
     1141 ccgcatatcc ctttatgtta tgttttgaag gcaccggagc ggaattatat gacgagcaat
     1201 cggacaggac atattccggt cgtgtcagca tgagcaatat gaagtttaaa aaagagtatg
     1261 tagatgattt gagttatttc agcggcggag cctacttata tctggtattg gagcttaccg
     1321 ataacaaaat tcccgaagat gtgagcttct atcaggagtt taccggtaaa ttgataagaa
     1381 gagaagcgca cgatgtattg attgtatcca gaagcgcagt ggatatggaa aagtcagacg
     1441 acccggagga accgtttgta tatcaggtgt ataagatatt accggataat acgatagaac
     1501 gtgtccgaat taagatcggt gatatcttcg atgcttatta cgaagttata gaagggttgg
     1561 aagaagggga taaggtagta ttaagatgag agaaaagaga gcaaaccttt ttattacaat
     1621 tattatatta ttaattttca cagctattct tacatcgtgt gctacccaag cggttactta
     1681 tgaagtaccg gtttttgata taccgaaaga agtttttacc actttaaagg tggaaaaaca
     1741 gaatatacat gattacgaaa gaatcaattt ggatgttcag agttcaggcg gaagcagagg
     1801 catagtattt acctattccg attttgaatc ggcaacactt attgtctccg aacgcgatgc
     1861 cgttgtgcag gaaggggatg ttcttgctgt caaaagcgat aaagctttag agtacgattt
     1921 gcggcttaaa ctggaagatc tggaactgaa acagttgtta tatgaggaac tttacagaaa
     1981 gcatctcaat accggtgagg gatactatga tatgcagttg gctttatcgg atatggaact
     2041 tgcacagttt aattatgaca agacattgga aaaattcgaa acgcttcaat ttaaggcacc
     2101 ttacagcgga acggttaaca atattatttc gacatacaag aatgagaaag atgatttcaa
     2161 taatgaatac aaagtatata ctgtaacata ttatattact cccgataatt acagtgtcag
     2221 tttacagata acacctgccg gatatcctta ttttgaagag atgggatata agtccggtga
     2281 tactcttcag atatatgata aagacgggaa aattcataat atgagaatca gcacgatgtt
     2341 caccagaaca accggagctg tcggttcaaa aacaaaaact gcttttgtag cttcactaat
     2401 aaatcctaat ataactacta t
//
LOCUS       DAOPWC010000243         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_46185_length_2415_cov_3.261017, whole genome shotgun sequence.
ACCESSION   DAOPWC010000243 DAOPWC010000000
VERSION     DAOPWC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46185_length_2415_cov_3.261017"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKJ65_05610"
     CDS             <1..802
                     /locus_tag="PKJ65_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41343.1"
                     /translation="IELFSEADDIYSAIKNSILGTSKFLYEEPQFLLQQEVSEIGSYF
                     SIIRAIAAGKTKLSEIASFLEVKATSLTKYLKTLIDLDIIERKVPITEKNPERSKKGL
                     YRIKDNFIRFWFLFVYPNRSYIERGNTEFVMQLIRKNLVTSHVSYVYEDVCREKMWDL
                     SDNGKLPFRVSKVGSWWNSKTEMDIAAINFDEKKIILGECKFWKDKVGMNILRVLEEK
                     AKTAVPESSAYSIYYILFSAAGFTDELKSYAQSRKDLLLFDDLLSKDK"
     gene            complement(871..>2415)
                     /locus_tag="PKJ65_05615"
     CDS             complement(871..>2415)
                     /locus_tag="PKJ65_05615"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HNZ41344.1"
                     /translation="TVAALRARGIRIGSTTGYTSEMMNGILHKVSAQGYSPDYWISAD
                     KTTKGRPYPYMIWNNLMHFGISDTREAVKVGDTTSDIAEGKNANCWTVGIIMGSSELG
                     LTRREVSALSKNELEEKKAIVRASYYKAGADYIIDDMNELLDVITDIDRKLNQNAARK
                     LLTPGPLTTRYSVKHAMLTDHCTWDDEYKAITTSVIKDITHISANDDYTTVLLQGSGT
                     YAVETMIHTLCRKDEKILFLVNGEYGKRMLTIADKSGKNYTYLSFDMTQPVNVKAVEK
                     ELQKHKQDISVVAFVHCETTTGIINPVEELTKLCKSYGKKVLVDAMSSFGAYDIDMPS
                     LDIDALASSANKCLEGLPGLAFVVVKKTLLETSSGNSSSHSLDLFDQYKCFCEANGKF
                     RFTSPTNILLALRQAIDDYKMEGGLKARKKRYMENHRVLVQELEELGIKSIIAPENQS
                     YIITTFELGNLDFFTLYQSLKSKGFVIYPGKLTSRPTFRLGNIGDVYPEDMVALADAI
                     KELYRI"
BASE COUNT          692 a          483 c          490 g          750 t
ORIGIN      
        1 tatagagctt ttttctgaag cggacgatat atattcggct ataaagaaca gtatcttggg
       61 tacatcaaag tttctatatg aagagccgca gtttctgctc caacaggagg tgtccgagat
      121 aggaagttat ttttcaataa tcagagccat tgccgcgggg aagactaaac tatcggaaat
      181 tgcctctttt cttgaagtca aggcgacaag tcttacaaaa tatcttaaga cccttattga
      241 tttggatatt attgagagga aggtgccgat taccgaaaaa aatcctgaaa gaagcaaaaa
      301 aggactttat agaattaagg ataatttcat acgtttttgg tttttgtttg tctatcccaa
      361 tcgtagttat atcgaaagag gaaacaccga atttgtcatg caactgataa gaaagaatct
      421 tgtcacctct catgtttcct atgtttacga agatgtctgc cgtgagaaaa tgtgggatct
      481 ttcagacaac ggcaagctgc cctttagggt ttctaaagta ggttcttggt ggaattccaa
      541 aaccgagatg gatattgcag caataaactt tgatgaaaaa aagataattt taggtgaatg
      601 taaattttgg aaggacaaag tcggtatgaa tatattaagg gtacttgagg aaaaagccaa
      661 aactgcagta ccggaaagct ccgcatacag catttattat atcttattca gtgcagccgg
      721 ttttaccgat gagcttaaaa gctatgcaca aagcagaaaa gacttactgc tatttgatga
      781 cttgttatcg aaagataaat aatatcatta accggaaccg gaaatgtcgg aattatatgt
      841 attcgcttat attggtttta tgcaagccga ttaaatccga tataattcct ttatcgcatc
      901 ggcaagcgca accatatctt cgggatacac atctccgata tttcctaatc tgaaagtggg
      961 tctggaagtg agtttgccgg gatagataac aaatcctttt gacttaagcg attggtacaa
     1021 tgtaaagaag tccagattac cgagctcaaa agttgttatg atatacgatt gattttccgg
     1081 tgcaataata gacttgatgc ccagttcttc cagttcttgc actaaaactc tatgattttc
     1141 catataacgc tttttccgag ctttaagtcc gccttccatt ttataatcat cgatagcctg
     1201 tctcaaggcc agcagtatgt tggtagggga ggtgaaacgg aacttgccgt ttgcctcgca
     1261 gaaacactta tattgatcaa ataaatcaag actgtgtgaa gaggagttgc ctgaacttgt
     1321 ctctaacagt gttttcttta ccactacaaa agccagcccc ggcaatcctt ccaaacattt
     1381 atttgcactt gaagccaatg catcaatatc cagactcggc atatcaatat cgtaagctcc
     1441 gaagctggac atggcatcca caagaacttt tttaccgtat gatttgcaga gttttgttaa
     1501 ttcttcaaca ggatttataa tgccggttgt ggtttcgcaa tgaacgaatg cgactacgga
     1561 aatgtcttgc ttgtgcttct gtaattcttt ttcaacagct ttaacattaa caggttgtgt
     1621 catatcaaaa ctgagatatg tataattttt gcccgatttg tcagcgatgg ttaacattcg
     1681 tttcccgtat tcgccgttta ctaaaaataa tattttttcg tcttttcggc agagtgtatg
     1741 aatcattgtc tctaccgcat atgtgccgct gccttgcaaa agaacggtgg tataatcatc
     1801 gttggctgaa atatgtgtaa tatcctttat taccgaagtt gtaatagcct tatactcatc
     1861 gtcccaggta caatgatctg tgagcatggc atgcttaaca gaataccttg tagtcagcgg
     1921 tcccggtgtc aataactttc gggcggcatt ttgattgagc tttcggtcta tatccgtaat
     1981 tacatccaaa agttcattca tgtcgtcgat tatataatcc gctcccgcct tgtagtacga
     2041 tgcacgaacg attgcttttt tctcttccaa ctcatttttt gacaaggctg aaacctctct
     2101 gcgggtaagt cccaactcgg acgaacccat aataatgccc actgtccaac aatttgcatt
     2161 tttcccctcc gctatatcag aagttgtatc tccgactttt accgcttctc tcgtatccga
     2221 aataccgaag tgcatgagat tgttccagat catgtacgga taaggtctgc ccttagtagt
     2281 tttgtcggca ctgatccagt aatcggggga gtagccttgt gccgaaactt tatgtaaaat
     2341 gccattcatc atctcggagg tgtagccggt agtggaacct atcctgattc cccgtgctct
     2401 gagagcggca accgt
//
LOCUS       DAOPWC010000244         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_46325_length_2411_cov_3.375637, whole genome shotgun sequence.
ACCESSION   DAOPWC010000244 DAOPWC010000000
VERSION     DAOPWC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46325_length_2411_cov_3.375637"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKJ65_05620"
     CDS             complement(<1..333)
                     /locus_tag="PKJ65_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ41345.1"
                     /translation="MYDVIIIGKGPAGAQAGIYLGRAGFKTLILYKDGGSLEKADYVE
                     NYYGFGKPVEAKKLLQEGVEQARRFNVEFKEDQVVMLLPSEQSVSVQGLKGEYVASYV
                     IIAAGMTRR"
     gene            complement(350..1090)
                     /locus_tag="PKJ65_05625"
     CDS             complement(350..1090)
                     /locus_tag="PKJ65_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41346.1"
                     /translation="MIFNPAKLLSKYYSSFISDDIGDTVIMFSKWAYKYTNIPYILLG
                     ILALAVVLALLSSLIFSGYMNIIHLTVKRIKTNFSHYLQGLKKGFFRCALVFFQMYLS
                     LLLFVAFIPLAFTPFFILKNSVADAGHDPTVVYILLAVLIFIIIAIFILIYMTFVFKF
                     PSIFHFSRYPIEKANAAVNARYWRTFAKSALLLLFLAGVLFIMYKIENKVLEFLVGFV
                     LYSIYFSFFAVFPFYTFDKLIEPYRVNS"
     gene            complement(1248..>2411)
                     /locus_tag="PKJ65_05630"
     CDS             complement(1248..>2411)
                     /locus_tag="PKJ65_05630"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ41347.1"
                     /translation="RLLMEGRTRQRADIMDRAATAEIDINYTNYIVCVAEFELAASQK
                     KLNIYDFSFYKAAIMSTIEENLEEIYKCYTFNLDNRIGILICSNQQIQRSILINKLND
                     VKSWVNKNMGLSLSIGIGNTCGSVEKVALSYISALDAMLYRVVLGKNVVVDAEQKINA
                     SKEKMVVEDFDNILKNSEEDMVFALRNVNQEEAKKIIEQIMLASRQIAANDVHQKERI
                     AFLLSVFIIRVIYSLEISNYRFYNKENDLYNEINEQISIDDMKIFISGIINEIIHAIE
                     ESDSKQNNFLVNKGLRYIKESVYEDVSLVTIAERLQIHPNYLSKIFKQVTDESFTDHV
                     IRFKMTEAKKLLKTTNAKVYEISDKLGYKDVAHFTKLFKKHFGISPTEYRQLL"
BASE COUNT          731 a          505 c          417 g          758 t
ORIGIN      
        1 tcttctggtc attcccgctg caattatgac ataagaagcg acatattccc cttttaatcc
       61 ctgtaccgaa acggactgtt cggaaggcaa caacatgacc acctggtctt ctttgaactc
      121 cacattaaac cgcctggcct gttcaactcc ttcttggagc agcttttttg cttcaacagg
      181 tttaccaaat ccgtaataat tctcgacata gtcggctttc tccaacgaac cgccgtcttt
      241 gtaaagtata agggttttga atcccgctct tcccagatag atgcccgctt gtgctcctgc
      301 aggtcccttg cctattatta tgacatcata cattttttgc ctccttgaat cagctgttaa
      361 ccctgtaagg ttcaataagc ttatcaaatg tataaaaggg aaaaaccgca aagaagctga
      421 aatatatact ataaagaaca aatcctacca aaaattccaa aaccttgttt tctatcttat
      481 acatgatgaa gagcactccc gcaaggaaaa gcagcagtaa tgcggatttg gcgaaggttc
      541 tccagtatcg cgcattgacg gcagcattgg ctttctcaat gggatatctg gaaaaatgga
      601 atatggacgg aaacttaaat acgaaagtca tgtatatcaa aatgaaaatg gcaatgataa
      661 taaatatcag caccgcaagt aaaatataca cgacggtagg gtcatgtccc gcatccgcaa
      721 cggaattctt caatatgaaa aacggggtaa atgccaacgg aataaaggct acaaacagca
      781 acagtgagag gtacatctga aagaatacca atgcacatct gaaaaatcct ttttttaatc
      841 cctgaagata atgagaaaaa ttcgtcttga ttcttttaac cgtcagatga attatattca
      901 tatatccgct gaatataagg ctggacaaca aagcaagtac taccgccaaa gccagaatgc
      961 ccagcagtat atacgggata ttggtatatt tatatgccca tttggaaaac atgataaccg
     1021 tatcgcctat gtcatcggat ataaacgatg aatagtattt ggaaagcagt tttgcggggt
     1081 taaaaatcat tattatgcaa caaactaaag atatacatgc cgtataaata ggtatcaaag
     1141 gcttttttat aaaatatact aatgaattac tgaaaaaatt catgtttcct ccattaaaac
     1201 attgtgatta tatcacaggg gtataccctt tgcaataaat cggtcggtta tagtaactgt
     1261 ctgtattcgg taggcgatat accgaaatgt ttcttaaaca gttttgtaaa atgtgcaaca
     1321 tccttatacc ccaatttgtc ggatatttca tacactttcg cattggttgt ctttaaaagt
     1381 ttttttgctt cggtcatctt aaaccgtatt acatgatcgg taaaggattc gtcggtaacc
     1441 tgcttaaata tcttactgag gtagttagga tgtatctgaa gcctctcggc aatggttacc
     1501 aatgaaacat cctcataaac actttccttt atatacctta atcctttgtt aaccaggaaa
     1561 ttgttctgct tgctgtccga ttcttcgatg gcgtgaataa tttcatttat tatgccggat
     1621 ataaagattt tcatatcgtc aatggatatc tgttcattaa tctcattata taaatcgttt
     1681 tccttgttat aaaagcggta attggaaatt tccagcgagt atatcaccct tataataaaa
     1741 accgaaagta agaaagctat tcgctctttc tggtgaacat cattggcggc tatctgcctg
     1801 gaggccagca ttatctgctc gattattttc ttagcttcct cctgattgac attccgcaat
     1861 gcaaaaacca tatcttcttc actgtttttc agaatgttgt caaaatcctc cacaaccatt
     1921 ttctctttgg aagcatttat tttctgctcc gcatcaacca ctacattttt acccaagacc
     1981 accctgtaaa gcattgcatc caaagcactg atatatgaaa gagcaacttt ttccacactt
     2041 ccgcaggtgt tacctatccc tatggataat gaaagcccca tgttcttgtt tacccagctt
     2101 ttaacgtcat tgagtttgtt gataaggatg cttctttgta tctgctggtt ggaacatatg
     2161 agtatgccta tgcggttatc cagattaaat gtataacatt tgtaaatctc ctcaagattc
     2221 tcctctatcg tactcatgat agccgcttta tagaaagaga agtcatatat gttcagcttt
     2281 ttctgagatg cggcaagctc aaattccgct acacatacaa tatagttggt gtaattaata
     2341 tctatctccg ccgttgcggc tctgtccatt atatcggctc tctgtcttgt tcttccttcc
     2401 ataagtaaac g
//
LOCUS       DAOPWC010000245         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47099_length_2383_cov_2.551117, whole genome shotgun sequence.
ACCESSION   DAOPWC010000245 DAOPWC010000000
VERSION     DAOPWC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47099_length_2383_cov_2.551117"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKJ65_05635"
     CDS             complement(<1..525)
                     /locus_tag="PKJ65_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41348.1"
                     /translation="MKGRKRKITMAVLLLLLAINLTSCALITLIKKNDATPGNSPFMQ
                     KISFDPYSVGMCLEDVNTDHAKYVADIDEFYTHFDSCVAKKHRSIFVRSSSQDSFSSL
                     DCLDEYGLKGCYRTIYSTNSGSYFAIYELEYYPGDSVVYAYLNNDRSLLSQKELELYR
                     FAVDFVNNRLDSKMS"
     gene            708..1778
                     /locus_tag="PKJ65_05640"
     CDS             708..1778
                     /locus_tag="PKJ65_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNZ41349.1"
                     /translation="MILKKDCKYDIMAVTSMGIRITPVNRQPVNVSNLYEMHATSAET
                     NVLNISSALGLKAKVLTKFVKDSPIALYIKGELRKRNIEFEGIDIEQGGPWGYRHQFN
                     IADSGFGVRGPRVLNDRAGEVGRTLSASDFDLERIFKEEGCRILHLSGLIAALSEESS
                     KCCLDIALAAKKYGTAISFDLNYRDSFWKGREKELREIFTKIASISDILIGNEEDFQL
                     ALGVKGPEAGGKAISKTDAFKEMITRISAQFKNVKVFANTLRQVENANLHLWGAIIYA
                     EGNWYVVEPREIPVLDRIGGGDGFVGGLLYGILKEWQPEKWIQFAWASGALATTLLTD
                     YAQPADEEVIWSIYQGNARVKR"
     gene            complement(1879..2082)
                     /locus_tag="PKJ65_05645"
     CDS             complement(1879..2082)
                     /locus_tag="PKJ65_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNZ41350.1"
                     /translation="MNVPKIVPIAELRDTKHVLQMCRETREPIIVTKNGYGELVIMSI
                     ETFTEMTEELNRLRKQTVKDSTY"
BASE COUNT          749 a          450 c          475 g          709 t
ORIGIN      
        1 actcattttg ctgtccagac gattgtttac gaaatccacg gcaaaacgat aaagttccaa
       61 ttctttctga ctgagcaatg atctgtcatt attgagatac gcatacacaa cactgtcacc
      121 gggataatat tccaactcat atattgcaaa atagctgccg ctgtttgtcg aataaatcgt
      181 ccggtagcat ccttttaatc catattcgtc caagcaatca agagaagaaa aactgtcctg
      241 tgatgacgag cgtacaaata tacttctgtg tttttttgca acacaggaat caaaatgggt
      301 ataaaattca tcaatatctg ctacatattt tgcatgatcg gtattgacat cttccaagca
      361 cattcctacg gaatagggat caaacgaaat tttttgcata aagggactgt tacccggtgt
      421 tgcatcattt ttctttatca aagttattaa tgcacaggat gtaagattta tggcaagtaa
      481 cagtaaaagc actgccatgg ttatttttct ttttctaccc ttcaagcaaa tatgcctcca
      541 gcacgcaaat ggtattatac accacttgca aaaagattta aacattataa gttgttgttt
      601 ctattgatta caagtatttt aataaaaacc cggcaacatt tccttatttt cccgtaatga
      661 tatataatat cggatataag tcagtaaatc attcggaggt gtattttatg attctaaaaa
      721 aagactgtaa atacgatatt atggccgtca caagcatggg tatcaggatt actcccgtca
      781 atcgccagcc tgtaaatgtg tccaacctgt acgaaatgca tgcaacaagc gcggagacca
      841 atgttttaaa catttcctcg gctttaggat taaaagccaa ggttttaaca aaattcgtta
      901 aggacagccc cattgctctt tacataaagg gagaactcag gaaaagaaat atagaatttg
      961 aagggattga tattgaacag ggaggtcctt ggggatatcg ccatcaattc aatatagcag
     1021 acagcggctt cggggtaaga ggacccaggg tgctgaatga cagagcgggc gaagtgggca
     1081 gaacattgtc cgcatcggac tttgatctgg aaagaatatt caaagaggaa ggttgcagaa
     1141 ttttacatct ttcgggattg atagcggctc tttccgaaga atcatcaaaa tgctgtctgg
     1201 atattgcatt agctgcaaag aaatacggaa ccgccatatc ttttgattta aattacagag
     1261 attccttctg gaaaggaaga gagaaagaac tccgtgaaat atttacaaag attgcttcca
     1321 tatcggatat attgatagga aacgaagaag attttcaact ggcactgggt gtcaaggggc
     1381 cggaagccgg aggtaaggca atttccaaaa ccgatgcatt caaagaaatg ataacaagga
     1441 tatcggcaca gtttaaaaat gtaaaagtat ttgcaaacac tttaagacaa gtggaaaatg
     1501 caaacttaca tttatggggt gccatcatat atgccgaagg taactggtat gttgtggagc
     1561 cgcgtgaaat tcccgttttg gacagaatag gaggcggaga cggatttgtc ggcggtctct
     1621 tatacggtat tcttaaagaa tggcaaccgg aaaaatggat acagtttgca tgggcaagcg
     1681 gagcactggc aactaccctt ttaacagatt acgctcaacc tgcggacgaa gaagtaatat
     1741 ggagtatata ccaaggaaat gcgcgcgtaa aaagatagaa gagcaccgag actcttctat
     1801 ttaaccgcat aatgccaaat ggtttataaa ataactttag accttaagtg gatacatgta
     1861 tacaacaatt taaatcagct aatatgtgct atcctttaca gtttgctttc tgagtctgtt
     1921 taactcttca gtcatttcgg taaaagtttc aatactcatt ataaccaatt cgccatatcc
     1981 gttctttgta acgatgatag gttctcttgt ttctctgcac atttgcagta catgctttgt
     2041 atctcgtaat tctgctatgg gaacaatctt cggcacattc ataccagtac atcctccata
     2101 ttaggatagt atactatgtg ctaaataatg tcaatgaaga atttaaaatt caccgcttat
     2161 ttatgcaaat atagtgcata tttaagggtg tttttataac atctttaaga ataatgtccg
     2221 ttactttctc ttaagattgc ttgtcttata ctcgaccaaa actttcagat ggtccgacag
     2281 cgcctgcatc tcccatcggg ctttctgtcc gtctcttgcc ttgagcgcct gatagatatt
     2341 attgtgcatt ttcatgcatt gctgtttcgc actcgcatca ggt
//
LOCUS       DAOPWC010000246         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47258_length_2378_cov_2.623332, whole genome shotgun sequence.
ACCESSION   DAOPWC010000246 DAOPWC010000000
VERSION     DAOPWC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47258_length_2378_cov_2.623332"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKJ65_05650"
     CDS             <1..545
                     /locus_tag="PKJ65_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ41351.1"
                     /translation="NLIYKSKKTVEIMVNYDPSMHYFTEWWKQLYGESEGKENKGIFP
                     AGVDNTADLHSMGQYIQEGIRNIFETVVYIKDDMSAITVPDIPDNSDGLDYIKNKSLK
                     YVNEMAMRGTKSAHVEGDVPVILIELEKMDAFTFGKLIYFFERACGISGYILGVNPFD
                     QPGVEAYKKSMFKLLGKPGY"
     gene            548..1657
                     /locus_tag="PKJ65_05655"
     CDS             548..1657
                     /locus_tag="PKJ65_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ41352.1"
                     /translation="MMKEKEKSVKKVKRASTGKLSSKAIVILSIVALFIICIVISVII
                     FVDSMHTSIYETPSPTPSQVVETITLPPSETPSTEPVETQVTETPTPTPMPMYKPQDD
                     IDFLLLFGLAEYDLADTIWIGIVNKTDNAIDFLSIPRDTYLRYKDRHNPDHWKINSYY
                     NNYVNDQWVKKSDSASSMNLMLACETLLNIDMDYYVRVSYDTIERIVDAMGGIEYDVP
                     FRMEYYDLTPGQELIIEIDEGLQVLNGENVVKFLRFRQGTGYDYISDIARINRQQDMI
                     KTIIKKAFTLSNISAVIDVAKNNIRTNFGKDQLTSFITFALSLNNEKISFHTLKGYED
                     MVNTQVKDSEGNDIIRSFYIYSDDEVRKQLISICE"
     gene            1915..>2378
                     /locus_tag="PKJ65_05660"
     CDS             1915..>2378
                     /locus_tag="PKJ65_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNZ41353.1"
                     /translation="MKISIPKGTKDILPGEIEKYKYIQTVFADMLENFGYSQIRFPVF
                     EYTELFQRGVGDTTDIVQKEMYTFNDKGGRSITLRPEGTASVVRSFIENGMSSNTQPV
                     KLYYDITTYRYENVQKGRYREFNQLGAEILGSDSYNADIEVLFMLYSLFTELG"
BASE COUNT          833 a          382 c          489 g          674 t
ORIGIN      
        1 gaaacctgat atacaagagc aaaaagaccg tagagattat ggttaattat gatccttcaa
       61 tgcattattt caccgaatgg tggaaacagc tttacggaga atcggaagga aaagaaaata
      121 aaggaatttt tccggcaggt gtagacaata ctgccgacct tcattcaatg ggtcaatata
      181 tccaggaggg aataagaaat attttcgaga ccgtcgttta cataaaagac gatatgtccg
      241 caataacagt tcccgatatc ccggacaatt ccgacggtct ggactatata aagaataaaa
      301 gccttaaata tgtaaacgaa atggctatga gaggcaccaa gtccgctcat gtggaagggg
      361 atgttcccgt tatattgatt gaattggaaa aaatggacgc atttacattc ggtaaactta
      421 tatatttctt tgaaagagca tgcggaataa gcggatatat cttaggtgtc aatccttttg
      481 atcaacccgg tgtggaagcc tataaaaaga gtatgttcaa attattgggg aaaccgggat
      541 attgattatg atgaaggaaa aggaaaaatc ggttaaaaaa gttaaaaggg caagtaccgg
      601 aaaattaagc agcaaagcta ttgtgatttt gagtatcgta gcactcttta ttatatgcat
      661 tgttatttct gtaataatat tcgtcgattc catgcatact tccatttatg agactccgag
      721 tcctacaccc agtcaggttg tagagacaat aacacttcca cccagcgaga caccttcaac
      781 ggaacctgta gagacgcaag taacggaaac tcctacaccg acaccgatgc ctatgtataa
      841 accgcaggat gatatagatt ttcttctgtt attcggattg gccgagtatg atctggcaga
      901 taccatttgg ataggaattg tcaataaaac ggacaatgca atagactttt taagcatacc
      961 cagagataca tatttgcgat acaaggatag gcataatccc gatcattgga agataaattc
     1021 ctattacaat aattatgtaa acgaccaatg ggttaaaaaa tccgattcgg catcatctat
     1081 gaatttgatg ttggcttgtg aaacattgtt gaatattgat atggactatt atgtaagagt
     1141 ttcatatgac acaatagaaa gaattgtgga tgccatggga ggtattgaat atgatgttcc
     1201 cttcagaatg gagtattatg atttgacacc cggacaggag cttataattg aaatagatga
     1261 aggattgcag gtcctgaacg gagagaatgt tgttaagttt ttacgatttc gtcagggaac
     1321 gggatatgat tatatctccg atatagcacg aataaacaga caacaggata tgattaaaac
     1381 cataattaaa aaggctttca cgttatccaa tatatccgcc gttatagatg ttgcaaagaa
     1441 taatataagg accaatttcg gaaaagacca gctgacctcg tttataacct tcgctttgag
     1501 cctgaacaat gagaagattt ccttccatac tcttaaaggc tatgaggata tggttaatac
     1561 tcaagtaaaa gattcggaag gcaatgatat tattcgcagt ttctatatat attccgatga
     1621 tgaagtgaga aagcagctga tatctatatg tgaatagctt tctgttgaag agaatataaa
     1681 atcatgagca ttactaacaa atcattaagc attgtatgtt tctatgatag atcgaagaaa
     1741 ccgccctatg cacggcaaag cttacgctct tgacaaatag ccgcttttgg tattaaatta
     1801 ttcaagataa aggatatatg caaaactaaa aaaggcgctt ttaaggcatg tatcaagatt
     1861 taatcgtctt ttacatttta tcggtaagag acttttttta acaggaggat aattatgaag
     1921 atatccatac cgaagggaac taaagatata ttacccggag aaatagagaa atataaatat
     1981 atacagactg tatttgcaga tatgctggaa aatttcggat attcacagat aagatttccg
     2041 gttttcgaat acacggagtt atttcaaaga ggagtaggtg atacaaccga tatagtgcag
     2101 aaagagatgt atacatttaa tgataaaggc ggcagatcca ttacattaag acctgaaggt
     2161 acggcatcgg tagtcagaag ctttatagaa aacggcatgt cgtccaatac ccaacctgta
     2221 aaactctact atgatattac gacatacaga tatgaaaatg ttcaaaaagg cagatacaga
     2281 gagtttaatc aactgggagc ggaaatctta ggttccgatt catataatgc ggatattgaa
     2341 gtattattca tgctttactc attatttacc gaattggg
//
LOCUS       DAOPWC010000247         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47339_length_2375_cov_3.323707, whole genome shotgun sequence.
ACCESSION   DAOPWC010000247 DAOPWC010000000
VERSION     DAOPWC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47339_length_2375_cov_3.323707"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKJ65_05665"
     CDS             complement(<1..594)
                     /locus_tag="PKJ65_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNZ41354.1"
                     /translation="MRVLFIGDIYGKTGRVILKNNIACLKEQYAPDYVIANGENTAGG
                     KGITPTLLQELLSMGADVVTSGNHIYARKEILPAFSDENIKLLRPLNYAETCPGKGYT
                     ILKNGCSPLCVINVSGRAFMEPCDNPFYAVESVLDQVKKEARNIVVDFHGEATSEKAA
                     FAWYFDGRVSAVIGTHTHVQTADERILPFGTAFITDAG"
     gene            719..2167
                     /locus_tag="PKJ65_05670"
     CDS             719..2167
                     /locus_tag="PKJ65_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ41355.1"
                     /translation="MKTPEKSILNRIKQPDIQKSIPFHMPGHKRNVNFNKHLNKIGIR
                     YDFTEIPQLDNLQTPEGIIKNVMDKAQLLWNTKKSFLSVNGSTCGINAAVATLADLKD
                     KIIVARNSHKSVYNAIELLDLEPVFITPAFNNKLNISGSINPETVYNAIFCNPDAKTV
                     LLTSPTYDGVISDIKAICDISHKMNIPVIVDEAHGAHLHLSNYFGNSAVSKNADIVIQ
                     SLHKTLPCLTQTAIAHICTDRIDVNKFQRYLSVFQTSSPGYPLLSSIESCIDLLLNNK
                     DKLYSDWIKNIDYIDNRISELNNIKAFYHGLSKNEVYGNIYAYDRSKLLLSTDNLSLN
                     GKQLFDMLMKDYGIICEMYTNSYVLAMLSMCDKKSSVRKLCNALEEIDLTCSENDAPS
                     FKNTQTYDIPKRCMSIREARSKRSTGIPIPEAQGHICAEYIWAYPPGIPYLIPGEKIT
                     REITDILKEAIDNGIDLKTDNNKQGYITVLCN"
     gene            2235..>2375
                     /gene="scfA"
                     /locus_tag="PKJ65_05675"
     CDS             2235..>2375
                     /gene="scfA"
                     /locus_tag="PKJ65_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HNZ41356.1"
                     /translation="MEKTKRIKTIETRNLVSSIKNGGCGECQTSCQSACKTSCGVANQ
                     KCE"
BASE COUNT          803 a          500 c          434 g          638 t
ORIGIN      
        1 tcccgcatcg gttataaaag cggttccgaa aggcaatatt ctttcatcgg cagtctgcac
       61 atgagtatga gtccctataa ccgccgatac tctgccgtca aaataccatg caaatgccgc
      121 cttttcactg gtggcttctc cgtggaaatc caccactata tttctcgctt ctttcttaac
      181 ttggtccagc accgactcta cggcataaaa aggattgtcg caaggctcca taaatgctct
      241 tcccgatacg ttaataacgc aaagaggaga acaaccgttt ttaagtatag tataaccttt
      301 tcccggacaa gtctctgcat agtttaacgg tcttaaaagc ttaatatttt catccgaaaa
      361 agcaggtaat atttcttttc ttgcataaat gtgatttccc gatgtaacaa catccgcacc
      421 catggataat aattcctgta aaagtgtcgg tgttatgcct ttaccccctg cggtattttc
      481 accgttggca ataacataat cgggagcata ttgctctttt aagcaagcaa tgttgttttt
      541 taatataact cttccggtct taccgtatat atcgccgata aataagactc tcattcaaac
      601 ctcgattcta ttgtttaatt ataatccatt cacacacatt tgagaagtaa ccgataacaa
      661 ccggttgcag cacttaacat tttgctatac tatttaccta tacaataatg aggatgtaat
      721 gaaaactccg gaaaaaagca ttttaaacag aataaagcaa ccagatatac aaaaaagtat
      781 accttttcat atgccggggc ataagagaaa tgtaaacttc aataaacacc tgaataaaat
      841 cggcatcagg tatgatttta ccgaaatacc gcagctggat aatctgcaaa ctcctgaagg
      901 catcattaaa aatgtcatgg ataaagcaca actgctttgg aataccaaaa aaagttttct
      961 ttcggtaaac ggaagcacct gcggaataaa tgcagctgtg gccacactgg cagacctgaa
     1021 agacaagata atagttgcaa gaaacagtca taagtctgtg tataacgcta ttgaacttct
     1081 ggacctggaa ccggtgttta taacccccgc atttaacaat aaactgaaca tatccggctc
     1141 aatcaatcct gaaacggtct ataatgcgat attttgcaat cccgacgcca agacggtgct
     1201 cttaacaagt cccacctatg acggcgttat aagcgatata aaggccattt gcgatatatc
     1261 acataagatg aatataccgg ttattgtaga cgaggcacac ggagcacatt tgcatcttag
     1321 caactacttc ggcaacagtg ccgtttcaaa gaatgcggac attgtaatac agagtctgca
     1381 taaaacatta ccctgcctga cacagacggc aatagcacat atatgcacgg atagaataga
     1441 tgttaataag tttcaacgat atctgtcggt ttttcagaca agcagtcccg gttatccgtt
     1501 actttcctca attgaaagct gtattgacct tttattaaac aataaagaca aactatactc
     1561 tgactggata aagaacattg actatataga taacagaata tccgaactta acaacataaa
     1621 agcattttat cacggtttat ccaaaaacga ggtatacggg aatatatacg catatgaccg
     1681 atccaaactg ttgctctcga cagataacct gtctttaaac ggtaaacagc tgttcgacat
     1741 gcttatgaaa gattacggta tcatatgtga aatgtataca aactcctatg ttttggccat
     1801 gctgtccatg tgtgacaaga aatcctccgt aagaaaactg tgcaatgctt tggaagaaat
     1861 agatttgacc tgctccgaaa atgatgcacc ctcctttaaa aacactcaaa catacgatat
     1921 ccccaaacgg tgtatgagta taagagaggc aagaagcaaa agaagtaccg gtatccccat
     1981 tcccgaagca caaggacata tctgtgcgga gtacatatgg gcgtatcctc ccggtatccc
     2041 ctacctcatt ccgggagaaa agataacacg ggaaataaca gatattctta aagaagcaat
     2101 tgacaacggt atagatttaa aaaccgataa caacaaacaa ggctatatca cggtattgtg
     2161 taattgacat attaccttat ttaatctaaa attatcctgt aaaatatgat tttgtaaaag
     2221 gatcggtaaa agaaatggaa aagacaaaaa gaattaaaac aatagagacc cgtaatttgg
     2281 taagcagtat taaaaacggc ggttgcggcg aatgccagac atcctgtcag tcggcttgca
     2341 agacttcctg cggagttgct aaccagaagt gtgag
//
LOCUS       DAOPWC010000248         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47580_length_2367_cov_3.940744, whole genome shotgun sequence.
ACCESSION   DAOPWC010000248 DAOPWC010000000
VERSION     DAOPWC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47580_length_2367_cov_3.940744"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKJ65_05680"
     CDS             complement(<1..324)
                     /locus_tag="PKJ65_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ41357.1"
                     /translation="MSKKATAVTGTTNQWQVTLTITGKDLVQSSNADIVLVIDRSGSM
                     SENSRMDNAKAAATFFINTLLTPGNTSRRIAIVSFATNVTINPGNVNDAFKGADQKQT
                     LLDVIN"
     gene            complement(968..1726)
                     /locus_tag="PKJ65_05685"
     CDS             complement(968..1726)
                     /locus_tag="PKJ65_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ41358.1"
                     /translation="MDLYRILDLYKVAIDECRPFEGEMLTQLRNYYRIGLTWSSNALE
                     GNTLTESETKVLLEDGLTVGGKPLRYTFEAIGHAQAYDFMFTLLNNRTITEKDVLMMH
                     QLFYASIEKEYAGKYRDMDVFISGSKYPVTETKRIQAEMDGLFQWIRNERNQFHPVTF
                     AAQLHKSFVFIHPFKDGNGRIARLLMNTALIQDGYLLAIIPPVLRHEYIELLERAHRD
                     DRPFEQFIVERVIESQKEIMRLLHIPIPKLDNSN"
     gene            2008..>2367
                     /locus_tag="PKJ65_05690"
     CDS             2008..>2367
                     /locus_tag="PKJ65_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNZ41359.1"
                     /translation="MQIVAINGGPRRGKISKTTMLLEAFLSGCQQGGAEVETINLREK
                     DIKMCTGCYSCWIKTPSQCALKDDMKEILLTLDKADVEVWATPLYFFGPTALFKNFVD
                     RTIPLAEPYIIEKDGLCT"
BASE COUNT          677 a          513 c          463 g          714 t
ORIGIN      
        1 atttatgacg tctaacaggg tttgtttttg atcggcaccc ttgaatgcat cattcacgtt
       61 gccggggttt atggtaacat ttgtggcaaa ggaaactatg gcaatgcgcc ggctggtgtt
      121 acccggagta agcagggtat taatgaaaaa tgttgcagca gctttggcgt tatccattct
      181 cgagttttca ctcatactac cggaccggtc aataaccaga acaatgtccg cgttggatga
      241 ttgcaccagg tctttgccgg taatagtaag cgtgacctgc cattgattgg tagtgcctgt
      301 caccgcggtt gcttttttgc tcaaggcaat ttggcctggg tcaggttcat tgtcctcggc
      361 caaaccatag cccggaaaaa agctgaataa aaacagcacg gtcactaaca gggctactat
      421 cttactgttt ctgagtctaa aattcatgtt gttactcctt tcctataacg tttttgctgt
      481 accgatgggc aaaccgctgt tgggatttcc gggtctgact ggaggtcgtt attagtactc
      541 ggtgaaagat agataataat ataatacata gaaatttata tacaaatata taggataatg
      601 tatgtatcta tgggcattta taggaatttt actttttcat tctccgtaca gcatgctacc
      661 ccaaaagaaa accgggaaaa caaaaagtct ccccggtcga atattaccta attcggttgt
      721 tacagcctgg ccgtccgctc gaaacaatct cttatctttt ttctgaatta gatttcatac
      781 ctttggatat aaatttaaaa acaaaatctc caattccaaa ttctttaccc aaataatttg
      841 ctgctcccat aagaagtcta gtaaacaata tcactataaa atagctataa cttttaagat
      901 gaaatagata taatcacccc caatatcaca atactatttg caggcaacgg ttctgaacat
      961 tttagtttca gttgctgttg tccagctttg gtattggtat atgcaacagt cgcatgattt
     1021 ccttctggga ttcgataacc cgctcaacaa taaactgctc aaaaggccgg tcatctctgt
     1081 gagctctttc gagaagttca atgtattcat gcctgagcac cggaggaatt atagctaaca
     1141 ggtatccgtc ctgtatcaga gccgtattca taaggagtct tgcaattctc ccatttccgt
     1201 ctttaaacgg atggataaag acaaaacttt tatggagttg tgccgcaaac gttaccgggt
     1261 gaaactgatt tcgttcattt ctaatccatt gaaataatcc atccatttcc gcctgtatgc
     1321 gttttgtttc ggtcacaggg tatttggaac cgctaataaa cacatccata tcacggtatt
     1381 ttcctgcata ttccttttct atactcgcat aaaaaagttg atgcatcata agaacatctt
     1441 tttccgtaat ggttctatta tttagcaacg tgaacatgaa atcataagct tgagcgtgac
     1501 cgattgcttc aaaggtatag cgcaagggtt ttcctcccac agtcaaacca tcttccagca
     1561 agactttggt ttcactttcc gtaagggtat tcccttcaag cgcattggat gaccaggtga
     1621 ggccaatacg atagtaattt ctgagttgtg ttaacatctc tccctcaaac ggtctgcact
     1681 catctatggc aaccttatac aaatcaagga ttctgtatag atccatttat tcaccctttt
     1741 catggtacat tatctcatat aataatatca taaaatcttc catattacat ggtaaaccgt
     1801 cacatcccta ttctcccttc ttatcctaac tttcacctca aattccccaa aacccaccat
     1861 tcccaacaag caggagggag cgagcgtcaa gggggcagtg ccttttcccg ataaacttac
     1921 cgggaaagca catcttaaat gtattataat ggaatctata gtacatagaa taggtcttat
     1981 tacctggata taaaaggggg aaataatatg caaatcgtag ccatcaacgg aggtcccaga
     2041 cggggtaaga tttctaagac tactatgctt ctggaggctt tcctgtccgg ttgtcaacaa
     2101 ggaggggctg aggtcgagac cattaatctt cgggagaagg acattaaaat gtgcaccggt
     2161 tgttatagtt gttggattaa aacgccgagc caatgtgcct tgaaagacga tatgaaagaa
     2221 atcctattaa ccctggataa agcagatgtg gaggtttggg ctactccact gtatttcttt
     2281 ggtcctaccg ccctgtttaa aaactttgtc gaccgcacca taccgctggc tgaaccctac
     2341 ataattgaaa aagatgggct ctgtacc
//
LOCUS       DAOPWC010000249         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47713_length_2363_cov_3.071490, whole genome shotgun sequence.
ACCESSION   DAOPWC010000249 DAOPWC010000000
VERSION     DAOPWC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47713_length_2363_cov_3.071490"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1343)
                     /locus_tag="PKJ65_05695"
     CDS             complement(48..1343)
                     /locus_tag="PKJ65_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41360.1"
                     /translation="MKLKLNYKRTFLVGLAFMSICMFWQMYDFVIPLILTNKFQMRVD
                     LANAIMALDNVLGLFMLPLFGVLSDKVNTKIGRRMPFILVGTAVAAIFTVVLPIAANS
                     NNLVLFMIALCLVLIAMATYRSPAVALMPDVTAKPVRSQGNAVINLMGTLGGTIVLAL
                     KMIIKPGEGWDYTTFFIVVASMMVISVMILFVTVNENKYRKEMEDINYGDPIIEEPGP
                     EVTEEKSDKLPKDVRKSMILLLASIFLWVFGYNAVTTAFSQYVVNFFNLPEGLAAGFL
                     MVATVTGFLTFLPVSYLTARIGRRKTILGALALGIILFTISFFIKEFTFFANFLFAGV
                     GVVAGSITVNTLPMVLEMSKGSTIGRYTGYYYTCTMSAQILTPVLMGRLFYLIGYQIL
                     FPYATLFLILSFITMLFVKHGDSKPQVKKNKLEQFDVED"
     gene            complement(1500..>2363)
                     /locus_tag="PKJ65_05700"
     CDS             complement(1500..>2363)
                     /locus_tag="PKJ65_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ41361.1"
                     /translation="KTYKTIMKSAENSGSLKKLETFIKIADALRLNNKIRRKLFSSVI
                     AGLGGNMRLMISGAAAISPTVSKFYRAVGIDFLQGYGLTECSPIICLNSDKDFVDESA
                     GKPLPGVEVVILDKDSEGIGEICAKGPNIMKGYMNMPEENAKAFEGGYFHTGDLGYMD
                     KNDFVYITGRKKSVIVTKTGKNIFPEELEQKLDRSKYVEECLVFGVTDKNGDSIITAS
                     VYPALQIFEEEFNNTDKKFIHDKIMELVQEVNSHVPTYKAIKKLVIRDNEFEKTTTRK
                     IKRYGDNYKAD"
BASE COUNT          687 a          516 c          424 g          736 t
ORIGIN      
        1 catccatatg ataatgagca ttaccgatgc gattaaccat tccattgtta atcctccaca
       61 tcaaactgtt ccaatttatt tttcttaacc tgtggtttac tgtctccgtg cttaacgaaa
      121 agcatggtta tgaaggaaag aataaggaac agtgtggcat acgggaacag aatctgataa
      181 ccgataagat agaacagtct tcccattaat acaggagtaa gtatctgtgc actcatggta
      241 catgtgtaat aatagcctgt atatcttcct attgtggaac ctttactcat ttccagaacc
      301 ataggcagtg tattaacggt aatggaacct gccacaacac ctacacctgc aaaaaggaaa
      361 ttggcaaaga aggtaaattc cttaataaaa aacgatatgg taaacagaat tattcctaaa
      421 gccaaagcgc ccaatatagt ctttcttcgt cctattcttg ctgtcagata agaaacaggt
      481 agaaaggtaa gaaatccggt taccgttgcc accataagga aacctgcagc aagaccttcc
      541 ggtaaattaa agaagtttac tacgtactgc gagaaagccg ttgtgacggc attgtaaccg
      601 aatacccaaa gaaagataga cgcaagaagc aatatcatac ttttcctgac atccttagga
      661 agtttgtcgg atttttcttc ggttacttca gggccgggct cttctattat gggatcaccg
      721 tagtttatgt cttccatttc ttttctgtac ttattctcgt ttacggtaac aaaaagaatc
      781 attacactta taaccatcat ggaagctacc actatgaaga aagtggtata atcccatcct
      841 tcacccggct tgataatcat tttcagtgcc aaaactatag taccgcccaa tgtacccata
      901 aggtttatga ctgcatttcc ctgcgatcgt acgggctttg cggtaacatc gggcataaga
      961 gctaccgccg gcgatctgta tgttgccata gcaataagca ccaaacacag agcaatcatg
     1021 aagagtacaa gattattgga gtttgccgct atcggcagca caacggtaaa tattgccgct
     1081 actgcggtgc cgaccagaat aaacggcatt cttctgccta tttttgtatt tactttgtca
     1141 cttaaaaccc cgaaaagcgg aagcatgaat aatcccaaaa cattatccaa agccattatg
     1201 gcgtttgcta aatccaccct catttggaat ttgttggtta gaatgagcgg tatgacaaaa
     1261 tcatacatct gccagaacat gcatatcgac atgaaagcaa gtcccacaag aaatgtcctt
     1321 ttgtaattca gtttgagttt catttatttt tcccccgatg taataataat gatattatag
     1381 cataagataa ctgtggaaaa aatcaattaa cggtaaagta ttttccacag tcacttatcc
     1441 tatgctatag tccgacacat gaatctttga aatttcaatg tcctatcgta acacaaatat
     1501 taatccgctt tatagttatc gccgtaacgt ttaatttttc ttgttgtggt cttttcaaat
     1561 tcgttgtccc ttataacaag cttcttaatt gctttgtatg tgggtacgtg tgaattgacc
     1621 tcctgaacaa gttccattat cttgtcatga atgaacttct tgtcggtatt gttgaattct
     1681 tcttcaaata tctgaagtgc gggataaacc gaagcggtta ttatactgtc tccgttctta
     1741 tcggttacac cgaataccag acactcttcc acatatttgc tcctatccag cttttgctcc
     1801 agctcttccg ggaatatgtt tttgccggtt tttgtgacta ttacggattt ctttcttcct
     1861 gttatgtata caaagtcatt tttatccata tagcctaagt ctccggtatg gaaatatcct
     1921 ccttcaaatg ctttggcatt ttcttccggc atattcatgt agcccttcat tatattggga
     1981 cctttcgcac atatttcacc tatgccctcg gaatccttat ctaaaataac aacttccact
     2041 cccggtaacg gttttcctgc cgattcatcc acgaaatcct tatcggaatt caggcagatt
     2101 ataggagaac attcggtaag tccgtatcct tgcagaaaat caatacctac cgctctgtaa
     2161 aacttggaga ccgtaggtga tattgcagcc gcaccggata tcatcaacct catatttccg
     2221 cctaaaccgg cgatgaccga agaaaacagt tttcttctta tcttattgtt gagacgaagt
     2281 gcgtcggcaa ttttaataaa tgtttcgagt ttcttaagac tgccggaatt ctccgccgat
     2341 ttcatgatgg tcttgtatgt ctt
//
LOCUS       DAOPWC010000250         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_47901_length_2356_cov_3.347675, whole genome shotgun sequence.
ACCESSION   DAOPWC010000250 DAOPWC010000000
VERSION     DAOPWC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47901_length_2356_cov_3.347675"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKJ65_05705"
     CDS             complement(<1..568)
                     /locus_tag="PKJ65_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41362.1"
                     /translation="MLRTAVIGMGPIGNNHAKAYKSCKQAELVAVCHIRKDRADAAKE
                     RYVVPAYYDAKEMLKAEKPDIVSVATGGFEYSSDHYIPTMQALNAGCHVLTEKPISNN
                     LQHGQEMVDLAKKLDRCFGIDLNHRFTPAARTAKKWQNEGLIGNLLFLNMALWIGRFQ
                     PHFDTEYYHLKALNPHSCDILRYFGGDVE"
     gene            complement(580..1959)
                     /locus_tag="PKJ65_05710"
     CDS             complement(580..1959)
                     /locus_tag="PKJ65_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ41363.1"
                     /translation="MAKKPNLLFFGIDSLRRDHMSLYGYNRLTTPNIDKYLKKGTVYN
                     NCFSPHIPTTPGYANMLTGFDCFGTDIVALRHKGDIAPGVKTLAEILSSHGYTTTNIG
                     FKNVSGRGFDNYIQYSGWSIEEHDKRSHKAESMNAVAIPELERLAAQDKPFFLFLRHM
                     DPHSPYLPPRPWERMFYQGNEFDPNNRSMEPVMAFKPFCDYFAEWIPEGCTDKDYVVA
                     QYDAAIAYMDTCIQSILQKLYDLGLENETLVVFTSDHGETLYDHDCYFDHHSLYDPTL
                     VVPLAFRFPGRVPEGVRVDDTCRLYDVTPTVLDILGIETRINFDGRSVFPPAFGQPIP
                     KLDEFYITECTWMRKHGWRTPKWKYIQALEPDFHFKPEVELYDLENDPMELWNIAKDR
                     PDIVMMLDKKMSDFISKREKEVGRTNPIYTNLTWHGLDLNRGFISSEEAYNSMHIGSA
                     KTAAKLQQK"
     gene            complement(2029..>2356)
                     /locus_tag="PKJ65_05715"
     CDS             complement(2029..>2356)
                     /locus_tag="PKJ65_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ41364.1"
                     /translation="GVDMDPSHIYRASELPEEAIAAVLPRMKHIHIRDCKGRGPGPGH
                     PSLQACGKGDINLFGYISKMVEAGYSGPVNLEVIGPEQDMVEATRIAAESYGYINACL
                     KILNAR"
BASE COUNT          566 a          560 c          499 g          731 t
ORIGIN      
        1 gttccacatc gcctccgaaa taccgcaata tatcgcagga atgcggatta agagccttaa
       61 gatgatagta ttccgtgtca aaatgcggtt ggaatctgcc tatccacagt gccatgttca
      121 aaaagagcag gttgcctatt aaaccttcat tctgccattt ctttgcggta cgggcagcag
      181 gtgtgaatct gtgatttaag tctataccga agcatctgtc taatttttta gccaaatcca
      241 ccatttcctg accgtgttgc aaattgttgg atatgggttt ttccgtcagt acgtgacagc
      301 ccgcgttgag tgcctgcatg gtggggatat agtgatcgga actgtattca aaccctccag
      361 tagccacgct tacaatgtcc ggtttctccg ctttgagcat ctcttttgca tcataatatg
      421 ccggaaccac atatctttcc tttgccgcat cggctctgtc tttccgaata tggcataccg
      481 ctacaagttc cgcctgcttg cagcttttat aggcttttgc atgattgttg ccgataggtc
      541 ccatgcctat gacggctgtt cttaacatga tatgcctcct tatttctgct gcaatttagc
      601 tgccgttttg gctgagccta tgtgcatgga gttataagct tcttccgagg atatgaagcc
      661 tctgttaaga tccagtccgt gccatgtaag gttggtatat atgggattgg ttcttccgac
      721 ttctttttcg cgcttactta taaagtcgct cattttttta tccagcatca taactatatc
      781 cggccggtcc tttgcaatat tccacagctc catgggatca ttttccaaat catacagttc
      841 cacttccggt ttgaaatgga aatccggctc caatgcctga atatatttcc acttgggagt
      901 tctccatccg tgttttctca tccatgtaca ttcggtgatg taaaactcgt caagcttcgg
      961 tatcggttgt ccgaatgccg gaggaaatac actgcgtccg tcaaagttta tacgtgtttc
     1021 tattcccaga atatccagaa ccgtaggcgt aacatcatac agtctgcagg tatcgtccac
     1081 tctcacacct tctggtactc tgcccgggaa acggaaagca agaggaacca ccaaagtggg
     1141 atcatataaa ctgtgatgat caaaatagca gtcgtgatca taaagtgttt ctccgtggtc
     1201 ggatgtgaat actacaagtg tttcattctc cagtcccagg tcgtaaagtt tctgcagtat
     1261 cgactgaatg caggtatcca tgtatgcaat agccgcatca tactgggcaa caacataatc
     1321 tttatcggta catccttcgg gaatccattc cgcgaaatag tcacaaaacg gtttgaatgc
     1381 cataacaggt tccatcgagc ggttgttggg gtcaaattca ttaccctgat aaaacattct
     1441 ttcccagggt ctggggggaa gataaggtga atgaggatcc atgtgccgta agaacaggaa
     1501 gaaaggctta tcctgtgcag ccagtctttc cagttccggt atagccactg cgttcatact
     1561 ctcggcttta tgggaccttt tgtcatgttc ctcaatactc cagccgctgt attgtatgta
     1621 attgtcaaat cctcttccgg aaacattttt aaatcctatg tttgtggtgg tatatccgtg
     1681 tgacgaaaga atctctgcca gtgttttaac accgggagcg atatctcctt tatgtctcaa
     1741 agctactatg tcggtaccga aacagtcgaa accggtaagc atattggcat atcccggagt
     1801 tgtcgggata tgaggtgaga aacagttatt atacaccgtt ccttttttca ggtacttgtc
     1861 gatattggga gttgtcagcc tgttgtagcc gtagaggctc atatgatctc ttcttaagct
     1921 gtcaattccg aagaataata gattgggttt ctttgccatg ttatctctct ccttatatat
     1981 attacttatt aaatcactca cagtgaataa tgttttaatt tcatcagctt accttgcatt
     2041 aagtatctta aggcaggcat ttatgtatcc gtaactttcc gccgctatac gcgtggcttc
     2101 caccatatcc tgttccggcc ctataacttc caaattgacc ggtccgctgt aaccggcttc
     2161 caccatcttg gatatgtaac cgaacagatt gatatcgcct ttaccgcaag cctgcaacga
     2221 cgggtgtccc gggcccggtc ctcttccttt gcagtcacgt atatgtatat gtttcatccg
     2281 gggtaatacc gccgctattg cttcttccgg caactccgaa gctctgtata tatgggaagg
     2341 gtccatatcc actccg
//
LOCUS       DAOPWC010000251         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_48386_length_2340_cov_2.929540, whole genome shotgun sequence.
ACCESSION   DAOPWC010000251 DAOPWC010000000
VERSION     DAOPWC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48386_length_2340_cov_2.929540"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKJ65_05720"
     CDS             complement(<1..565)
                     /locus_tag="PKJ65_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ41365.1"
                     /translation="MILKKVELQGFKSFATRTVLEFNKGITAIVGPNGSGKSNIADAI
                     RWVLGSQSSRQLRGGSMSDMIFSGTQYRRPVGFAEVSLFFDNKDKHLAVDFEDVKITR
                     RLYRSGESEYSVNDKSCRLKDIQMMFFDTGVGKEGYSIIGQNEIKDIVDSNSLERRGI
                     FEEAAGIMKYKQKKLEALRKLTNTEENL"
     gene            complement(555..1265)
                     /gene="rnc"
                     /locus_tag="PKJ65_05725"
     CDS             complement(555..1265)
                     /gene="rnc"
                     /locus_tag="PKJ65_05725"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ41366.1"
                     /translation="MEKVIDKSIASVEKAIDYSFKDKSLLSTALKHSSYDKTENYEKL
                     EFLGDSVVSLVISDFLFTTHNYLQEGEMTVTRAYAVCEESLSKVSKRLGIDKYILIGS
                     SAANSAVNTNKSVIADAFESLIGAMYLDSGLEYVRPFIISNLNEFILEYIKSGDNNDH
                     KTKLQHFVQSRYQEEPKYLVKGEKGPPHDKTFLVEVIITGEPYGKGIGKTKKEAQQAA
                     AKDALENIEKTGLDNYDS"
     gene            complement(1391..1618)
                     /gene="acpP"
                     /locus_tag="PKJ65_05730"
     CDS             complement(1391..1618)
                     /gene="acpP"
                     /locus_tag="PKJ65_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ41367.1"
                     /translation="MIFEKVKAIIVEQLGVDADEVTMESSFVENLGADSLDIVELIMS
                     MEDQFKITIPDTEAENITTVGAAVKFIENNR"
     gene            complement(1715..>2340)
                     /locus_tag="PKJ65_05735"
     CDS             complement(1715..>2340)
                     /locus_tag="PKJ65_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HNZ41368.1"
                     /translation="IRPALGALIPSGEKFALLIDAGLNSNLRAESYVQFAKFGSEYMK
                     IVTKCENPSIGLLNIGTEDTKGSAEVKEANLLLKQSGLNYYGSIEGNSIVEGIVDVIV
                     TDGFTGNVSLKLLEGTASYIFNKMKAVFNMNIFHRLAAYVIRPDMKKFKDNLNPDVMG
                     GAPILGVNGLILKSHGSSNAKTIKNVVLKAVNLAENDIVGNIMRVVC"
BASE COUNT          644 a          549 c          350 g          797 t
ORIGIN      
        1 caaggttctc ttcggtgttt gtaagtttgc gcaaagcttc cagtttcttc tgtttatact
       61 tcatgatacc ggcagcttct tcgaaaatac ctcttctttc gaggctgttg ctgtctacaa
      121 tatcttttat ttcattctgc ccgatgatgg agtacccctc tttacctaca ccggtatcga
      181 aaaacatcat ctgaatatct tttaaacgac agctcttatc gttaacggag tattccgatt
      241 cgccggagcg gtacagtctt ctggtaatct tgacatcctc aaaatccact gccagatgct
      301 tatctttgtt atcaaagaaa agagaaactt cggcaaaacc cacaggtctt ctatactgag
      361 ttccggagaa tatcatgtcg ctcatactgc caccccttaa ctgcctggaa ctctgcgagc
      421 ctaaaaccca tcggattgca tcggcaatat tactctttcc gctcccgttg ggacctacta
      481 ttgcggttat acctttatta aactccagca ccgttcttgt tgcgaaggat ttaaatccct
      541 gcaattccac ttttttaaga atcataatta tctaaacctg ttttctcaat attctccaaa
      601 gcatctttcg cagcagcctg ctgtgcttcc ttctttgttt ttcctatacc ttttccgtac
      661 ggttcccccg taattataac ctcaacaagg aatgttttgt catgaggcgg tcctttttct
      721 cctttaacca gatatttcgg ctcttcctga tatctggact gcacaaaatg ttgcaatttc
      781 gttttgtgat cattattatc acctgacttg atatattcta aaataaactc attcaaattg
      841 gaaataataa aaggtcggac atattccaat cccgaatcca gatacattgc tccaatcaat
      901 gactcgaaag catcggctat aaccgatttg tttgtattaa cggctgagtt tgctgccgat
      961 gaaccgataa gtatatattt gtctattccc aaccgtttac ttacttttga taatgattct
     1021 tcacacaccg catatgccct tgttacggtc atctcgcctt cctgcaaata gttgtgagtt
     1081 gtgaaaagaa aatccgaaat aaccagcgaa acaaccgaat ctcccaaaaa ctccagtttt
     1141 tcgtaatttt ccgttttgtc gtatgagctg tgttttaagg cggtagacag caatgattta
     1201 tccttaaaag aataatctat agctttctct accgatgcta ttgatttatc aatcactttt
     1261 tccatttaat ctacatccga aataaacata tctctgataa ttccgtagta tcaagttgca
     1321 atatcggcct cattttctaa agatctttaa atcttaagaa aataaggcta ttgaaatagc
     1381 cttgattctc ttacctgttg ttttcaataa attttacagc ggcacctaca gtagtaatgt
     1441 tctccgcttc ggtgtcgggt atggtaatct taaactgatc ttccatagac attataagtt
     1501 ccacaatgtc caatgaatcg gctcccagat tttccacaaa tgaagattcc attgttactt
     1561 cgtccgcatc cacacccaat tgttctacga taatcgcttt taccttctca aaaatcattt
     1621 tatcctcccg tctcacgatg gacgtatttt taatattcat catattattc catcgttaat
     1681 attaaccttt tttatattaa aacgcaaata gcgtttaaca tacaactctc attatattcc
     1741 ctacaatatc attttctgca aggttcaccg ccttaagcac cacattcttt attgtttttg
     1801 cattggagct tccgtgtgat ttcaatatta aaccgtttac tcccagtatc ggtgcacctc
     1861 ccattacatc gggatttaaa ttgtctttaa atttcttcat atcaggtctt atgacataag
     1921 cggcaagtct gtggaaaata ttcatattaa atacagcctt catcttattg aaaatatatg
     1981 aagctgtacc ttctaaaagt tttaatgaca catttcccgt aaaaccgtcg gtaacaataa
     2041 catccacaat accttccact atactgttcc cttcaatgct gccgtaatag tttaatcccg
     2101 actgttttaa aagcagattt gcctctttta cttccgcgct tcctttagtg tcttccgttc
     2161 cgatattcaa cagtcctatt gaaggatttt cacactttgt aactatcttc atgtattcgg
     2221 aaccgaattt tgcgaactga acataacttt ccgcacgcaa attggagttc agaccggcat
     2281 caataagtaa ggcaaacttt tctcccgaag gaatcaatgc ccccaaagcc ggacgtatta
//
LOCUS       DAOPWC010000252         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_48508_length_2336_cov_3.029811, whole genome shotgun sequence.
ACCESSION   DAOPWC010000252 DAOPWC010000000
VERSION     DAOPWC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48508_length_2336_cov_3.029811"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKJ65_05740"
     CDS             complement(<1..1299)
                     /locus_tag="PKJ65_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ41369.1"
                     /translation="MPYAMSVIVSRAIPEIDGLKPSHRKLLYTMYKMGLLKGDRTKSA
                     NVVGQTMKLNPHGDQAIYETMVRMTRGNEALLHPFIDSKGNFGKQYSRDMSYAASRYT
                     EVKLAPISAEMFRDIDKDCVPFTPNYDGTLMEPVMLPSAFPNILVNASQGIAVGIASN
                     LPSFNLAEVCDCIIAYLKDRDIYLGDYLKCLDFSTGGYAIYDKAEMENIFTNGRGRIR
                     LRAKYNVDKKGTHIEIVEIPYTTSAEAIIESIIALVKSSKIKEITDVRDETDISGLKI
                     TIDVKKGTDIDRLMAILYAKTPLEDGFSCNNNVLIEGKPLLLGIRDIIDYWIMFRVEC
                     IRRQLRYDLAKDRERLHLLLGLERVLLDIDEAIRIIRNTEMESEVVPNLMEAFRIDNL
                     QAEFIAEIKLRHLNREYILKRLDDIKDLNKEIKEAEETLKS"
     gene            complement(1387..>2336)
                     /locus_tag="PKJ65_05745"
     CDS             complement(1387..>2336)
                     /locus_tag="PKJ65_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNZ41370.1"
                     /translation="LVNSIFQDAFTEFLKHELEVYFIENKSEALKIADQVLLNKRSAE
                     QAEKTKLNLKKKLGKGLDMNNRVKKFVDCRSKDADKREIYIVEGDSALGSAKMGRDAE
                     FQAIIPVRGKILNCLKAEYNRIFDSDIIIDLLKVLGCGVEIKTKANKKISQFDMKNLK
                     WSKIIICTDADVDGFQIRTLILTMIYRLMPTLIEAGKVYIAESPLFEITHKKDTYFAY
                     SEAEKNEIIEEIKGNEKKNGQSPRITIQRSKGLGENEPDMMWLTTMNPATRRLIKVMP
                     TDVKDMDYMFDLLLGDNLQGRKDYIQEYGYKYTDMLDVS"
BASE COUNT          548 a          561 c          408 g          819 t
ORIGIN      
        1 gctcttcagt gtttcttccg cttcttttat ttccttattc aaatctttaa tgtcatccag
       61 tctctttaat atgtactccc tgttgagatg acgcaacttt atctccgcta taaactccgc
      121 ctgaaggtta tctattctga atgcttccat caaattggga acaacctcgc tttccatttc
      181 ggtattacgt attatacgga tggcttcgtc aatatccagc aacacccttt ccagtcccag
      241 taaaagatgc aaccgctctc tgtccttggc taaatcatat cgcaactgtc ttctgatgca
      301 ttccacacgg aacattatcc agtaatctat aatatccctt attcctaaaa gcagcggttt
      361 gccttcgata agcacattat tattacagga aaaaccgtct tccaaaggtg ttttggcata
      421 cagtattgcc atcaacctgt ctatatccgt tccttttttt acatcaatgg ttatcttaag
      481 ccccgatatg tcggtctcgt cacggacatc ggtaatctct ttaattttac tgctcttaac
      541 cagtgctatt atactttcaa taatggcttc cgccgatgtc gtatagggta tttccactat
      601 ttctatatgg gtaccctttt tatcgacatt gtattttgct ctcagcctta tccgtccgcg
      661 tccgtttgtg aatatattct ccatttccgc tttatcgtat atggcatagc cgcctgtgga
      721 gaaatccaga cacttgagat aatcccctaa ataaatatct ctgtctttca gatatgcaat
      781 tatacaatca cagacttccg caagattgaa agaaggcaga ttgcttgcaa tacctaccgc
      841 aataccctgg cttgcgttta ctaaaatatt gggaaatgcc gacggcaaca tgaccggttc
      901 cataagcgtt ccgtcatagt tgggtgtaaa cggcacgcag tctttgtcta tgtcgcggaa
      961 catctccgca cttatgggag ccagtttgac ttcggtatat ctgcttgcgg cataactcat
     1021 atccctggaa tactgttttc cgaagttacc tttggaatct atgaacgggt gaagaagcgc
     1081 ttcattccct cttgtcattc tgaccatggt ctcgtatatg gcctggtcac cgtgcggatt
     1141 caacttcatg gtctgaccga ccacatttgc agacttggtt ctgtcgcctt tcaaaagccc
     1201 cattttgtac attgtgtata gcagtttcct gtgtgaaggt ttcaatccgt ctatttccgg
     1261 aatggcacgc gaaactatta cgctcatggc atatggcatg aaattggttt ccagtatgtc
     1321 ggttatgagt tggggcttag ggtgatttac attgttatta ttatcttttt tacctttttt
     1381 catgtattat gacacatcca gcatatcggt atatttatac ccgtattcct gaatataatc
     1441 cttccttccc tgtaagttat ctcctaacag caagtcgaac atataatcca tatccttgac
     1501 atcggtaggc attactttta taagccttct tgtggcagga ttcattgtgg taagccacat
     1561 catatccggt tcattttctc ccaacccttt ggatctttgt atggtaatcc tgggagactg
     1621 cccgtttttc ttttcattgc cttttatttc ttctataatc tcgttcttct ccgcctcgct
     1681 gtatgcaaag tatgtatctt tcttatgcgt tatctcgaaa agcggtgatt ccgcaatgta
     1741 taccttgccc gcttctatca gggtaggcat cagtctgtat atcatggtca gtataagagt
     1801 tcttatctgg aaaccgtcca catcggcatc ggtgcagatt attatctttg accacttgag
     1861 gtttttcata tcaaactgac ttatcttctt gttggctttt gtcttaatct ccaccccgca
     1921 acccaatacc ttaagcaggt ctataatgat atcgctgtcg aatatcctgt tgtattcggc
     1981 ttttaagcag ttgagtattt ttcctcttac cggtattata gcctgaaatt ccgcgtctct
     2041 tcccatcttt gccgaaccta aagcggaatc accttccact atatatatct ctcttttatc
     2101 ggcatctttg cttctgcaat cgacgaattt cttgacacgg ttgttcatgt ccagaccttt
     2161 acccaatttc tttttcagat taagcttggt cttctccgcc tgctctgcgc ttcttttatt
     2221 taaaagaacc tgatcggcta tcttgagggc ttccgacttg ttctctataa aatatacttc
     2281 cagttcatgc tttaaaaact ccgtaaaggc atcctggaat attgaattaa ccagac
//
LOCUS       DAOPWC010000253         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_48574_length_2334_cov_2.975867, whole genome shotgun sequence.
ACCESSION   DAOPWC010000253 DAOPWC010000000
VERSION     DAOPWC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48574_length_2334_cov_2.975867"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="hisD"
                     /locus_tag="PKJ65_05750"
     CDS             complement(<1..911)
                     /gene="hisD"
                     /locus_tag="PKJ65_05750"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNZ41371.1"
                     /translation="MIPIFDKDKAGFIAKVRKDGFLDNKEVYDIVYKIMQDIRKDKDK
                     ALMQYTGKFDKTDIAPEDMLVTKEEIKKAYKEIDKDLLKVIQQAVLNVATYSIKQKEK
                     SFLWNKEEGVKIGQKVTPLRSVGIYIPGGTAPLISSVIMNAVPAYVAGVKEIVMCTPP
                     PLEAARIVAANESHVTKIYRAGGAQAIFAMAFGTETIPKTDKITGPGNIYVAYAKKMA
                     YGYCGIDMVAGPSEIMIIADETANPRFVAADMLSQAEHDKLSISSLLTTSKKLAKEVI
                     KEITVQAKELERSDIINESIKNNAAIY"
     gene            complement(895..1545)
                     /gene="hisG"
                     /locus_tag="PKJ65_05755"
     CDS             complement(895..1545)
                     /gene="hisG"
                     /locus_tag="PKJ65_05755"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNZ41372.1"
                     /translation="MEYLKIALPKGRLAVQTVELFKKAGIPCTDIDDHSRKLIHTDQS
                     NKIKIFLVKAADVPVYVEYGAADMGVAGKDTLMEESKNVYDVLNLGFGACRMCVAGET
                     KYLNRTDEIMNKRVATKYPKIASEYYRNVKNENVEIIKLNGSVELAPLCNLSELIVDI
                     VESGKTLQANGLVVLEKICDISAHLIVNKVSMNMKTDKISYIISALKKELSNDSDI"
     gene            complement(1533..>2334)
                     /locus_tag="PKJ65_05760"
     CDS             complement(1533..>2334)
                     /locus_tag="PKJ65_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNZ41373.1"
                     /translation="AKRVGINDISIQIGQVEFFNGLAKEASLTQKESEIISKMIDSKD
                     SFGISEYLEGRKIDKNIYNHLTAITGYFGDKSLIDRLLETKLNLKSKQALNNLKQILD
                     ILDKMGLSGYVTVDLSMVKKLGYYTGMIFNGNTYQMGFPVLAGGRYDNLAEKMGKKLE
                     ATGFSLSLEMAIKALERQGQTEELPVMPAIVSFSESMRDCVLSVTKDIEEDKNIQVLP
                     DSDDLERYMKERNISRMARCIAPDHVIIHSNGKTEKTDFAKWRALWNI"
BASE COUNT          552 a          568 c          398 g          816 t
ORIGIN      
        1 atatatatgg cagcgttatt cttaatactt tcattaatga tatcgcttct ttccaactct
       61 tttgcctgta cggttatctc ttttattact tccttcgcca gtttctttga tgtggtaagt
      121 aatgaagata ttgagagttt gtcatgctcg gcctggctca gcatatcggc ggcaacaaat
      181 ctcggatttg ccgtttcatc ggcaattatc attatttccg aaggccccgc caccatatct
      241 ataccgcagt atccgtatgc cattttcttg gcataggcaa catatatgtt acccggcccg
      301 gttatcttat ccgttttcgg tatggtttcg gtgccgaatg ccattgcaaa tattgcttgc
      361 gctcctcccg ctctgtatat ctttgttaca tgcgattcat tagcggcaac aatacgggca
      421 gcttctaaag gaggaggtgt acacatgact atttctttta cgcctgcaac atatgcgggg
      481 actgcattca taatgactga agatatgaga ggtgcggtgc ctcccggtat gtatatgccc
      541 acacttctta acggtgtgac tttctgccct atttttacgc cctcttcttt attccataaa
      601 aaacttttct ctttctgttt tatggaatat gtcgccacat tcagtaccgc ctgctgtatt
      661 acctttaaaa gatccttgtc tatctctttg taggcttttt taatctcttc ttttgtaaca
      721 agcatatcct caggcgcaat atcggttttg tcgaacttac cggtatattg catcaatgct
      781 ttatctttat cttttcttat atcctgcata atcttgtata caatatcgta aacttcttta
      841 ttatccagaa atccgtcttt tcgcaccttt gctataaaac ccgctttatc cttatcaaat
      901 atcggaatca ttgcttaact cctttttcaa tgctgagatt atataggata tcttgtcggt
      961 tttcatgttc atacttactt tattcactat gaggtgagcg gaaatgtcgc aaatcttctc
     1021 cagcaccaca agaccgttgg cctgcagcgt ctttccgctt tccactatgt ccacaataag
     1081 ttccgaaaga ttacataacg gagccagttc caccgatccg ttcaacttga ttatctccac
     1141 attctcattt ttgacatttc tgtaatattc gcttgcaatc ttgggatatt tggtggcaac
     1201 cctcttgttc attatctcat cggttctgtt aagatactta gtctctcccg ccacacacat
     1261 cctgcaggca ccgaaaccta agttaagaac atcataaaca ttttttgatt cttccatcag
     1321 agtgtctttt cccgcaactc ccatatcggc ggcaccgtat tccacataaa ccggcacatc
     1381 ggccgctttt accagaaata tcttgatctt gtttgattga tcggtatgta tgagctttct
     1441 ggaatggtca tctatatccg tacagggtat ccctgctttt ttgaaaagtt ccacggtctg
     1501 taccgccagt cgccctttgg gtagcgcaat ctttaaatat tccataatgc cctccacttt
     1561 gcaaaatcgg ttttctccgt tttgccgtta ctgtggatta taacatggtc gggcgctata
     1621 cagcgtgcca tccttgaaat attacgctcc ttcatatatc tttcaaggtc gtcactgtcc
     1681 ggcaacacct gtatgttttt atcttcctca atgtcttttg ttacagataa aacacaatct
     1741 ctcatacttt ccgaaaaaga cacgattgca ggcataacag gcagctcttc agtctgtccc
     1801 tgtctctcca aagccttgat ggccatttcc agactcaagg aaaagccggt agcttccagc
     1861 ttctttccca ttttttccgc cagattgtca tatcttccgc ccgcaagtac gggaaagccc
     1921 atctgatatg tgttgccgtt gaaaatcata ccggtgtaat aacccagctt tttaaccatg
     1981 gataaatcca cggtaacata tccgcttaaa cccatcttat ccagtatgtc cagaatctgc
     2041 ttaagattgt tcaaagcctg ttttgatttt aagttaagtt ttgtttcaag taatctgtca
     2101 atcagtgatt tatccccgaa gtatcccgtt atagcggtta agtggttgta tatattctta
     2161 tctatttttc ttccttccag gtattccgag ataccgaaac tgtccttgct gtctatcatc
     2221 ttggatatta tttcactttc tttctgtgta agagaagctt ctttggccag accgttgaaa
     2281 aactccacct gccctatctg aatagatatg tcattaattc ctactctctt tgcc
//
LOCUS       DAOPWC010000254         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_48686_length_2330_cov_3.737582, whole genome shotgun sequence.
ACCESSION   DAOPWC010000254 DAOPWC010000000
VERSION     DAOPWC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48686_length_2330_cov_3.737582"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKJ65_05765"
     CDS             <1..330
                     /locus_tag="PKJ65_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ41374.1"
                     /translation="PVFFKFKGGKGILISLSVLLMTDWRVALIGLGIFIIIVGLTRYV
                     SLGSITAMFLSPLFAWLLNHEPFFIISMGFLAALTIFMHRSNVKRLLNGTENKLSFNK
                     TGNGEGK"
     gene            327..1328
                     /locus_tag="PKJ65_05770"
     CDS             327..1328
                     /locus_tag="PKJ65_05770"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNZ41375.1"
                     /translation="MSNLIVINTSFNPWYNLALEEYLLDKVTDYDNILFLWQNANTVV
                     IGRNQNAWRECRTELLESEGGKLARRSTGGGAVYHDSGNLNFSFILPRSSQDFIIQAG
                     IITKAVGKFGINAYVNGRNDIVTDQGKFSGNAFTYRKNRALHHGTVLVDVDREKLGRY
                     LQVSKEKMQIKGIKSVQSRIVNLKEYDSGITVERVRDAIMQAFAEEFGQCEIFDCSKE
                     IDIFDRKFDMAEVHRTYEIYSSWEFRYGEAPKFDIEWANRFSWGEVVIHLTLKNGVII
                     KSRIYSDALDELFIANIYQCFDNVPFKKKAMAEAVLKQHPDSPMAKDIAEYILSKEM"
     gene            1328..1720
                     /locus_tag="PKJ65_05775"
     CDS             1328..1720
                     /locus_tag="PKJ65_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNZ41376.1"
                     /translation="MFFKDLHIKDIADIPVLSLAYIGDAVYDLYVREHLLVKGCFKPT
                     DLHKMAVVYVNAKGQKQSLMKIMDILTEKELNCVQRGRNAKSRSKPKNADAADYSYAT
                     ALEALVGYLYINKEYERLDYLLGIMLDI"
     gene            1717..2013
                     /locus_tag="PKJ65_05780"
     CDS             1717..2013
                     /locus_tag="PKJ65_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HNZ41377.1"
                     /translation="MMEEKNTVIAGRNPVLEALKKDCLISRILVSVGEKEGSILKILS
                     EARKKNIPVKQVSNTKLDSIIPAHQGVVAYISPIEFYEISDLLDVAKEKKEPPS"
     gene            2037..>2330
                     /locus_tag="PKJ65_05785"
     CDS             2037..>2330
                     /locus_tag="PKJ65_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ41378.1"
                     /translation="MHNLGAIARSAEAAGCHGIIIPLHRSAPVNAAAMKASAGALLHI
                     KVARVTNLNAAIEQLKKQGLWICGTDKDANKQYYDADLKGPLAVVIGSEGAGMS"
BASE COUNT          744 a          421 c          564 g          601 t
ORIGIN      
        1 cccgtgttct ttaaattcaa gggcggaaaa ggcatactga tttcactttc cgtgctactg
       61 atgaccgact ggagagtggc acttataggg ctggggatat ttataataat cgtggggctg
      121 acgagatatg tttcattagg ttccattacc gcaatgtttc tttctccgct atttgcctgg
      181 ttgcttaatc atgagccttt ctttatcata tcaatgggct ttttagccgc tcttaccata
      241 tttatgcaca gaagtaatgt caagagactg ctgaacggta ccgaaaacaa actgtctttc
      301 aacaagaccg gaaacgggga aggtaaatga gcaacctcat tgttataaac acctcattca
      361 atccgtggta taatcttgcg ctggaagaat atcttttaga taaagtaacc gattacgata
      421 acatattgtt tctatggcag aatgccaata cggtagtaat aggcagaaat cagaatgcct
      481 ggagagaatg ccgcacggaa ctgttggaat cggaaggcgg aaaactggcg agacggtcca
      541 caggaggggg agcggtttat cacgattcgg gcaatctgaa tttctctttt atacttccca
      601 gaagcagtca ggattttatt atacaagcag gtataataac taaagcggtc ggtaaattcg
      661 gtataaatgc atatgtcaac ggaagaaatg acatagttac ggatcaaggc aaattctccg
      721 gaaatgcttt tacatacaga aaaaacagag ccttgcatca cggtactgtg cttgtggatg
      781 tagaccggga gaaattgggc agatacctgc aggtcagcaa agaaaaaatg caaataaaag
      841 gcattaaaag cgtgcaaagc aggattgtca atctcaaaga atatgacagc ggcataacgg
      901 tagagcgggt acgtgacgca ataatgcagg cttttgcgga agagttcggc caatgcgaga
      961 tatttgactg ctcaaaagag atagatatat ttgaccgcaa attcgacatg gcggaagtgc
     1021 acagaacata tgaaatatat tcctcctggg agttcagata cggtgaagca cccaagtttg
     1081 atatagagtg ggcaaacaga ttttcctggg gagaggtggt catccatctt acattaaaaa
     1141 acggtgttat aattaaaagc aggatatatt ccgatgcttt ggacgagctg tttatagcta
     1201 atatatatca atgtttcgac aatgtacctt ttaagaaaaa agctatggca gaagccgtgc
     1261 tgaaacaaca tcccgactca ccgatggcaa aagacattgc ggaatatatt ctaagtaagg
     1321 agatgtaatg ttttttaagg atttgcatat taaagatatt gccgatatac ctgttttaag
     1381 tctggcttac ataggcgatg ccgtatacga tctttatgta agggagcatc ttttggtaaa
     1441 gggatgcttc aagcctaccg atcttcataa aatggcggtt gtttatgtaa atgcaaaagg
     1501 ccagaaacag agtcttatga agataatgga tattttgacg gaaaaagaat taaactgtgt
     1561 gcaaagagga cgaaatgcaa aatccaggag caaacctaag aatgccgatg cagcggacta
     1621 ttcctatgct accgctttag aggctcttgt gggatacctt tatatcaaca aagaatacga
     1681 aaggcttgat tacctgttgg gtatcatgtt ggatatatga tggaagaaaa aaataccgtt
     1741 atagcgggaa gaaatcccgt attggaagcc ttaaagaagg attgtctcat aagccggata
     1801 ctggtaagtg tcggtgaaaa agaggggtct atactgaaaa tactttcaga agcgagaaag
     1861 aagaatattc cggtaaagca ggtatccaat accaagcttg actccattat tccggctcat
     1921 cagggtgttg tggcatatat atccccgata gagttttatg aaatatccga tcttctggat
     1981 gtggcaaaag aaaaaaaaga gccgccttca tagtaatact ggatcagatt gaggatgtgc
     2041 ataatctggg agcaattgcc agaagtgccg aagctgcggg atgtcacggc attataatcc
     2101 cccttcacag gtcggctccc gtaaatgccg ccgcaatgaa agccagtgca ggtgccctgt
     2161 tgcatataaa ggtggccaga gtgaccaatc tcaatgcggc tatagagcag ctgaagaagc
     2221 aaggtttatg gatatgcgga acggataagg atgcaaataa gcaatattac gacgccgatc
     2281 tgaaaggacc gcttgccgta gttataggtt ccgaaggagc cggtatgtcc
//
LOCUS       DAOPWC010000255         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_48724_length_2329_cov_3.100264, whole genome shotgun sequence.
ACCESSION   DAOPWC010000255 DAOPWC010000000
VERSION     DAOPWC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48724_length_2329_cov_3.100264"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..424)
                     /locus_tag="PKJ65_05790"
     CDS             complement(176..424)
                     /locus_tag="PKJ65_05790"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNZ41379.1"
                     /translation="MNDSYCVIAFGNTHMAIKVQKTLNEKGFSINMMPTLRAITAGCG
                     LSIRFSSEDLTNIQKAVSEINIEPSLHQFYTVENNMGE"
     gene            complement(445..1704)
                     /gene="saoX"
                     /locus_tag="PKJ65_05795"
     CDS             complement(445..1704)
                     /gene="saoX"
                     /locus_tag="PKJ65_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263598084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HNZ41380.1"
                     /translation="MNKRILACALAIMMLTVVLTGCQDSSLQKNVVKVDYDVNEDVAP
                     DLAKLSAIPDADKEFVIQMGYNDCDHMVAAIIGENAGIYKALGLNVVVTKSGQTAAAM
                     ASGDMVAGYSGFSSMIKNANKGAPVFMPAGNHLGGSKYFVIKDSVESAEEIIGSRITV
                     GESDMYSPYWLSYCEQVGIPIDYTKYQGFDMGADDAIVAMKAGQLDGFMCCDPFGSVA
                     EMEGIGKIIGTEWGAHVSDDLETGWGLHCGYFINTDFAEEHPELTTRLVLAHCLAIQY
                     MYLHPYNAGMMFADDFGVDPAVGLRTMYLKLCAEGRTLSWDINETNIKNYTDYWHSYG
                     IPEDKIPEVKDLPSFFNLKYLEACGIESFKSFMEDNKINEKFPIRDSYLAWLEKAEEI
                     DGIDHSSTVGKTVEKWNNNEIVSKLLK"
     gene            complement(1792..>2329)
                     /locus_tag="PKJ65_05800"
     CDS             complement(1792..>2329)
                     /locus_tag="PKJ65_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_except=(pos:complement(1963..1965),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41381.1"
                     /translation="TIIGFPLGMLMGYSPFVMRTIAPFINSFRQIPTTSWVPLAIVWF
                     GLGDGPTIFVIMFHAIFTIILNTVAGVQSISKDFYNAARSMGAKTLDLVKDVVLPGSL
                     SGLITGIRISLGAAWMSVVUAEFIATNSGFGYIIKQAQLDMITEKVMAYMILAGVLGF
                     AQDRILLVIETFLIRWKR"
BASE COUNT          681 a          544 c          422 g          682 t
ORIGIN      
        1 ccctcctggg aataagggga gcagcgatta tttcccccta tcccgagata agcaatgatg
       61 tggacagact cagcgatttg gagtatatag aaaaatatat gtaagaagca gcggggaagc
      121 agcagggacg gttctttttg ctttcaaaag caaaaagaac cgtccctgct gctttctatt
      181 ctcccatatt attctcaacc gtataaaatt gatgtaatga tggttcaata ttaatttcag
      241 aaacagcctt ttgtatattt gtgagatcct ctgaggaaaa ccgtatagaa agaccacagc
      301 ctgcggtgat cgctctcaaa gtcggcatca tattaatact gaaacctttt tcgttaagtg
      361 ttttttgaac cttaatagcc atatgagtgt tgccaaaggc aattacacaa tatgaatcat
      421 tcaaagtgat gacactcctt attactattt tagcagcttc gatacaattt cattattgtt
      481 ccacttttct acggtcttac ctacagtgct ggagtgatcg ataccgtcaa tttcttctgc
      541 cttttcaagc catgccaagt aagagtcccg tattggaaac ttttcattaa tcttattgtc
      601 ttccataaaa ctcttaaaac tttctatacc acaggcttcg agatatttta gattgaagaa
      661 ggacggcaaa tcctttactt cgggtatttt atcttccggt attccatagc tatgccagta
      721 atccgtgtag ttttttatgt ttgtctcatt gatatcccag gataaagtac gcccttcagc
      781 acagagtttc aaatacattg tacgaagacc tactgccgga tctacgccaa agtcatcggc
      841 aaacatcatt cctgcgttat atgggtgcaa atacatatac tgaatggcaa gacaatgtgc
      901 caacaccaat cttgtagtta actcggggtg ttcttctgca aaatccgtgt tgataaagta
      961 tccgcagtgt aagccccaac cggtctccaa atcatctgaa acgtgggcgc cccactcagt
     1021 accgattatt ttgcctatac cttccatctc agcaactgac ccaaagggat cgcagcacat
     1081 aaagccgtct aactgaccgg ctttcattgc aactatagca tcatcagcgc ccatatcaaa
     1141 cccttgatat ttagtataat caattggtat accaacctgt tcgcagtagc ttagccagta
     1201 aggagaatac atatcacttt cacctacggt tattcttgac ccgattattt cttccgcaga
     1261 ttcgacacta tccttaataa caaaatattt ggagccgcct aaatgattac ctgcaggcat
     1321 aaataccgga gcgcccttat ttgcattttt tatcattgaa ctgaagcccg agtacccggc
     1381 caccatatca ccggaagcca tagcggcagc agtctgcccg ctcttagtaa caacgacgtt
     1441 cagaccgaga gctttgtaaa taccggcgtt ttcaccgata atggcagcga ccatatgatc
     1501 acagtcgtta taccccattt gtataacaaa ctctttatct gcatcgggaa ttgccgaaag
     1561 ctttgcgagg tctggagcaa catcttcatt aacatcataa tctactttta ctacgttttt
     1621 ctgtaagctg ctatcctgac atccggtcaa tactactgtc agcatcataa tagcaagcgc
     1681 gcatgcaagt attcttttat tcatataaat tctccctttc tatatgtttt gtatctcatc
     1741 tatttcctca cgggaaatcg tcactgcaac ataatatgga aaacttccgt attaccgttt
     1801 ccaccttatc aggaatgtct caattacgag taaaatcctg tcttgtgcga aacccagaac
     1861 cccggccagt atcatgtaag ccataacctt ttcggttatc atatctaatt gtgcctgttt
     1921 gattatatat ccaaagccgg aatttgttgc aatgaactcc gctcagacta cggacatcca
     1981 tgccgcaccc agagaaatac gtataccggt aatcagcccc gaaagggaac cgggaagcac
     2041 cacatcttta accaagtcta aggttttggc gcccatactg cgggcggcat tgtaaaaatc
     2101 tttgcttata ctttgcaccc ctgctactgt atttaatata atagtaaaaa ttgcatggaa
     2161 cataatgaca aaaatcgttg gtccgtcacc taatccaaac catactattg ccagtggaac
     2221 ccaagaagtt gtaggaatct ggcggaatga atttataaaa ggtgcaatgg ttcgcataac
     2281 aaagggtgaa tagcccatca acatacctaa gggaaaacca atgattgtt
//
LOCUS       DAOPWC010000256         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_49234_length_2312_cov_4.351794, whole genome shotgun sequence.
ACCESSION   DAOPWC010000256 DAOPWC010000000
VERSION     DAOPWC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49234_length_2312_cov_4.351794"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKJ65_05805"
     CDS             complement(<1..574)
                     /locus_tag="PKJ65_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41382.1"
                     /translation="MEKKRKRRQAFKEKAAIILLTVLYLVISLTNLGNTKAPVNGWTP
                     QKAGSVVYVDFEKQETIERITYLCGLGDTWYNVTALDLYYKDASGYYVHFVTLERRAS
                     TFLKWLYTDLEQPVTTDCVKVVSGIYQDKEEDYIKGTRGEIMEVCFFASGKDTPIPIS
                     SVRPTDPHKDEKSVNLFDEQDLYVYEPSYMN"
     gene            complement(698..1336)
                     /locus_tag="PKJ65_05810"
     CDS             complement(698..1336)
                     /locus_tag="PKJ65_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ41383.1"
                     /translation="MLTDNIKWLGHAAFRVDGSKTIYFDPFRIDEGKKADIILVSHKH
                     HDHCSKEDILKILKPDTLIVTDADSAEILQLDNMIIVKPYDSISVEGIEIQALPAYNI
                     TKPHHPKENGWLGFLVNIDGKTIYHAGDTDCIEEMRDITTDVALIPIYPDNKYVMTPD
                     EAAQATYYINTKVAVPMHFGVLGGSKGDALTVAKKGNPEIQVVILESQKNSF"
     gene            complement(1326..>2312)
                     /locus_tag="PKJ65_05815"
     CDS             complement(1326..>2312)
                     /locus_tag="PKJ65_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ41384.1"
                     /translation="VQTITVIVTTIVILIFGEITPKMVANDNPNKFAVNVTWLLKVFI
                     FIFKPIVFTVTKMTEKLSVIWKPKKEEPTVTEDELLSIVETIEEEGGFTEKESDLIRS
                     AIEFSDDRAFEILTPRVDVFAIDINDEPEDIIRAVVDEEYSRVPVFEGTIDNIIGILS
                     TKKLLKAAVKKGAQFDIRSMIREPLYIYKSMDISSVMEEFKAKRAHMAVVVDEFGGTM
                     GIVTLEDVLEQLVGDIWDETDDMSEDTVETAEGTYEVVGDMLLDDFLEMIDFDEELES
                     ESTTVGGFVTERLGRLPLENDRVEFDGHVITVLEMANLRVERIKVEMRKDAD"
BASE COUNT          582 a          562 c          407 g          761 t
ORIGIN      
        1 tgttcatata tgaaggctca taaacataca ggtcctgttc gtcaaacagg ttgacggatt
       61 tttcgtcttt atgcggatcg gtcggtctga ccgaacttat gggaatagga gtatctttac
      121 ctgaagcaaa gaaacatacc tccatgattt ctccccttgt ccctttgatg tagtcttctt
      181 ccttgtcctg ataaatgccg gaaacaacct ttacacaatc ggtggtaacg ggttgttcca
      241 aatccgtata aagccacttg agaaatgtgc tggctcttct ttccagggtg acgaaatgaa
      301 cataatatcc cgatgcatcc ttatagtata agtccagtgc cgtcacgtta taccaggtat
      361 cacccagacc gcaaagatat gttattctct caattgtttc ctgcttttca aaatcaacat
      421 acacgaccga cccggctttt tgcggtgtcc atccgttgac cggcgccttt gtgtttccga
      481 gattggttaa agatattaca agataaagca cggtaagcag aataatcgcc gccttttcct
      541 taaatgcctg ccttcttttc cttttctttt ccataaatgc tttatcccga gtactttcag
      601 gtaggaagtg ttgctctctc attttgcctg ttataataac ctcaatctgt tgcttctgta
      661 tatatgatta tatccttaca gggtataaaa taaaaggcta aaagctgttt ttttgacttt
      721 ccaaaataac tacctgtatt tcgggattac cttttttagc aaccgttaaa gcatcgccct
      781 ttgaaccgcc caacactccg aaatgcatgg gaaccgcaac ttttgtgttt atgtaatatg
      841 tggcctgtgc cgcttcatcg ggagtcatga catatttgtt atcgggatat atagggataa
      901 gtgcgacatc ggtcgtaata tcccgcattt cctctataca gtcggtatcg cctgcgtggt
      961 atattgtttt accgtcaata ttaaccagaa aacccagcca tccgttttcc ttggggtgat
     1021 ggggttttgt aatgttatat gccggtaatg cctgtatctc tattccttct acggaaatac
     1081 tgtcatacgg tttcactatt atcatattat ccagttgtaa aatttccgca ctgtccgcat
     1141 cggtaacaat caaagtatcc ggtttcaata tctttaatat atcctctttg gaacaatggt
     1201 catggtgttt gtgggaaacc aatatgatat ctgctttttt gccttcatct attctgaacg
     1261 gatcaaaata tatcgttttg ctgccgtcca ccctgaaggc ggcatgccct aaccacttta
     1321 tattatcagt cagcatcttt tctcatctcc acttttattc tttccactct caagtttgcc
     1381 atttccagta cggttattac atgaccgtca aactccaccc tgtcattttc taagggaagt
     1441 cttcccagcc tctcggtcac aaaaccgccg accgtggtgg attccgattc caattcttca
     1501 tcaaaatcta tcatttccag aaaatcatcc agcaacatgt ccccgacaac ctcataggtt
     1561 ccttccgccg tttccaccgt gtcctcactc atgtcgtcgg tttcatccca tatatccccg
     1621 accagctgtt ccagaacatc ctccagagtg actatgccca tagtacctcc gaattcatcg
     1681 acaacaacag ccatatgtgc tctttttgcc ttgaactctt ccataacgga ggatatgtcc
     1741 atggacttat atatgtataa aggttcacgt atcatggagc ggatatcaaa ctgtgctccc
     1801 tttttcacgg ctgctttcag caacttcttt gtacttaata tacctattat attatctata
     1861 gtaccttcaa aaaccggtac tctggaatat tcctcatcca ctaccgcccg tattatatcc
     1921 tcgggctcat catttatatc tatggcaaat acatccaccc tcggggtaag aatctcaaat
     1981 gctctgtcat cggaaaactc tattgccgac cgtataaggt cgctttcttt ttcggtaaac
     2041 ccgccctctt cttctattgt ctccactata cttaacaact cgtcttcggt aaccgtaggt
     2101 tcttcttttt taggtttcca tatcacggaa agcttttccg tcattttggt aaccgtaaac
     2161 actatgggtt tgaagatgaa tataaatacc ttaagcagcc aggtaacatt cacggcaaat
     2221 ttgttgggat tgtcgtttgc aaccatcttg ggtgttattt caccgaatat aaggattact
     2281 atggtggtaa ctataaccgt tatggtctga ac
//
LOCUS       DAOPWC010000257         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_49399_length_2306_cov_3.549089, whole genome shotgun sequence.
ACCESSION   DAOPWC010000257 DAOPWC010000000
VERSION     DAOPWC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49399_length_2306_cov_3.549089"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKJ65_05820"
     CDS             <1..491
                     /locus_tag="PKJ65_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ41385.1"
                     /translation="PGLINALYEVGITMNDVSPKYVIIGETRSYSFEKIEKAVQLVQR
                     GAKLIGTNPDRTGPTEKGIMPATGALAVPIEIATGKEVYYIGKPNPLMMRHALKKLGT
                     DVKNTIIIGDRMDTDIVAGIESEIDTVLVLSGVTNHQNIQNFSYRPKYIYANIGEIIK
                     KR"
     gene            680..2239
                     /gene="rho"
                     /locus_tag="PKJ65_05825"
     CDS             680..2239
                     /gene="rho"
                     /locus_tag="PKJ65_05825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ41386.1"
                     /translation="MNEVEILRSKSIADLRVIAKTLGVKSVTVMKKAELIEKILEITK
                     PEELNIEKEQPKEEAVVSEAAKILEAVTQETPKKPRYLLSKMAKESEVAKPVKEEKKA
                     RHVIKSHDSATIAPPVQAQSTSFQETADSQPDGDLPEEYEDGQYDQVCGVLEVLPDGY
                     GFLRTDNYSYGPKDVYVAPNQIRKYGVKTGDKIFGTGKPKKDNEKFQALQHVIAINDE
                     DPAKVFTRKSFDTLTPIFPEERLTLENNPKDYSTRIIDLIAPIGKGQRGMIVSPPKAG
                     KTVLLKKIANSITLNHPNVELIVLLIDERPEEVTDMQRSVKGEIVYSTFDKVPENHVK
                     VSEMVLERAQRMVEHGKDVVILLDSITRLARAHNLTMTPTGRTLSGGLDPGALHAPKK
                     FFGSARNIEFGGSLTVIATALVETGSRMDDVIYEEFKGTGNMELHLDRKLSERRIFPA
                     IDINKSGTRREELLLGKKELESIWTIRRAMSNMGTAEVTEWMIDKLMKTKNNAEFVSS
                     INIKQTAVMDY"
BASE COUNT          803 a          408 c          561 g          534 t
ORIGIN      
        1 aaccgggatt gataaacgct ttatacgaag tgggaattac aatgaatgat gtaagtccga
       61 agtatgtgat aataggggag accagatcgt attcctttga gaaaatagaa aaagccgtgc
      121 agttggtgca aaggggagca aaacttatcg gtaccaatcc cgacagaacc ggtcctaccg
      181 aaaaaggaat aatgcccgca accggagcgt tagcggtacc gattgagata gctaccggga
      241 aagaagtcta ctacatagga aaacccaatc ctctcatgat gaggcacgca ttgaaaaagc
      301 tgggaaccga tgtaaagaac accataataa taggcgaccg tatggatacc gatatagttg
      361 ccggtataga aagtgagata gataccgttt tggttttaag cggagttacc aatcatcaga
      421 atatccagaa cttctcttac agacctaaat acatatatgc aaatataggt gagattataa
      481 aaaaaaggta gatttaagac aaaatagtgc gtatttcggt gtgggcggaa tatgtgcaac
      541 atgtatgtct tctgcgaaga attaaagtcg aaaacatgca agaaatgttt gacaaaatac
      601 ttcgtgtatg atattgtttg aattcggaaa gaacaaatct atactctaaa tataaaaaaa
      661 tactttagtg aggatattaa tgaacgaggt tgagatttta aggtctaaat ccattgcgga
      721 tttgcgggtt attgccaaaa cgctgggcgt caagagcgtc acggttatga aaaaggcgga
      781 attgattgaa aagattttgg aaataaccaa accggaagaa ctgaatattg agaaggaaca
      841 accgaaagaa gaagcggttg tatccgaggc ggctaaaata ctggaagcgg tgacacagga
      901 aactcccaag aagcccagat atcttttgtc caaaatggca aaagaatcgg aggtagcaaa
      961 acctgtaaaa gaggaaaaga aggcaaggca tgttataaaa tcacatgact cggctaccat
     1021 tgctcctccg gtgcaggcac agagtacaag ttttcaggaa acagccgatt cgcagccaga
     1081 cggcgatttg cctgaagaat acgaagacgg tcaatatgat caggtttgcg gggtgttgga
     1141 agtgttgccg gacggatacg gtttcttacg taccgataat tactcttacg gtccgaagga
     1201 tgtgtatgtc gctcccaatc agatacgcaa gtacggagtt aagaccggcg ataagatatt
     1261 cggtaccgga aaacctaaaa aagataatga gaaattccag gctcttcagc atgtaatagc
     1321 cataaatgac gaggatccgg caaaagtttt caccagaaaa tcttttgata cgcttacccc
     1381 cattttcccg gaagagcggc ttacattgga gaataatccg aaagattatt caacaagaat
     1441 aatcgacctt attgctccga taggaaaagg gcagagagga atgatagtat ctcctcccaa
     1501 agccggtaaa accgtattgt tgaaaaagat agcaaacagc attaccctta atcatcccaa
     1561 tgtggaactt attgttctgc tgatagatga aaggcctgag gaagttaccg atatgcagcg
     1621 gtcggtaaag ggagagattg tttattccac atttgataaa gttcccgaaa accatgtcaa
     1681 ggtatcggaa atggttttag aaagagctca gaggatggtg gaacacggaa aagacgtggt
     1741 aatacttctt gacagtataa caaggcttgc cagagcgcat aaccttacaa tgacccctac
     1801 cggaagaaca ctttcgggag gactggatcc cggtgcgctt catgcaccca agaaattctt
     1861 cggttcggca agaaacattg agttcggagg aagccttacg gttattgcaa cagcccttgt
     1921 ggaaacggga agcagaatgg atgacgtcat ttacgaggaa ttcaagggta ccggtaatat
     1981 ggagttgcat ctggacagaa aactttccga aagaaggata ttcccggcaa tagacattaa
     2041 taagtcgggt accagaagag aggaactgct gttgggcaag aaagaactgg aaagtatctg
     2101 gaccataagg cgtgcaatga gcaatatggg tactgccgaa gtaacagaat ggatgataga
     2161 taaactgatg aagaccaaga ataatgcgga atttgtcagc agcattaata taaaacagac
     2221 ggctgtcatg gattattgat tgaaatcaga tgcttgagtg caaagttaaa ttccagtata
     2281 aaagaaactc tcaaaaaaat ggattt
//
LOCUS       DAOPWC010000258         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_49535_length_2302_cov_2.893636, whole genome shotgun sequence.
ACCESSION   DAOPWC010000258 DAOPWC010000000
VERSION     DAOPWC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49535_length_2302_cov_2.893636"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /gene="uvrB"
                     /locus_tag="PKJ65_05830"
     CDS             complement(<1..1429)
                     /gene="uvrB"
                     /locus_tag="PKJ65_05830"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNZ41387.1"
                     /translation="MSKRDKEFRSDKFVLQSDFRPTGDQPAAIETLANGVLSGYKHQT
                     LLGVTGSGKTFTMANIIEKVQKPTLVIAHNKTLAAQLCSEFKEFFPDNSVEYFVSYYD
                     YYQPEAYIPSTDTYIEKDMSINDEIDQLRHAATSALLQRRDVIIVASVSCIYGLGSPA
                     DYEDLMLYVGVGMTLDRDDVIKKLVEIQYSRNEMNFKRGTFRAKGDSLEIAPSNSANV
                     GYRISFFGDEVEKITQFDSLTGEITGYVNHLAVFPASHFVSKDTKLKRATEDIRAELQ
                     ERVAYFENQGKIVEAYRLNQRTNYDLEMIRNIGFCSGIENYSRHIDGRKEGQPPATLL
                     SFFPEDFLMFIDESHVTIPQIGGMNKGDRSRKKNLIDFGFRLPSAYDNRPLTFEEFKE
                     KQNQCIYVSATPGDYELGISEQVAEQIIRPTGLTDPKIEVRPVKGQVDDLLFEIRDTV
                     SRKNRVLVTTLTKRMAEKLTEYYSSL"
     gene            1513..>2302
                     /locus_tag="PKJ65_05835"
     CDS             1513..>2302
                     /locus_tag="PKJ65_05835"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HNZ41388.1"
                     /translation="MENKRKSVFWIFIKSFTDMHAYKHFVYKNFSKAFLYILLFTFLL
                     SVIFSAIFIASIKDYTTQLITQLEQNTPKFTIKNGELVIENDQPVILAETDDKGTLII
                     IDKEYTGSVTKYEEYDTVIVLGQKEIYLSNSAIRYSIGYESVLGQKQEVTSEMLFGRV
                     SSSKPFLYLFFFASMFIFLIGNYLFYTLFVALIVNTVRRIRRKQANLGISLQLSAYAL
                     TLPAIIYLIWIFTSLITIPIEPLIFVLAVIIAYKGVDAYYKSIDE"
BASE COUNT          657 a          504 c          413 g          728 t
ORIGIN      
        1 ccaaagaaga atagtactcg gtcagttttt ccgccattct ttttgttaag gttgtaacca
       61 atacacgatt tttccttgac acggtatcgc gtatctcaaa aagcaaatca tccacctgtc
      121 cttttacggg gcgtacctct atcttcggat cggtaagtcc cgtaggtcgt ataatctgct
      181 cggctacctg ctccgatatc cccagctcat aatcgccggg agttgccgac acataaatac
      241 attggttctg tttttcctta aattcttcaa aagtcagcgg cctgttgtca tatgcagaag
      301 gcagccggaa tccgaagtca atcagattct tcttcctgga ccggtcgcct ttgttcatac
      361 ctcctatctg aggaattgtt acatgcgact catctatgaa cattaaaaaa tcctcgggga
      421 agaaagaaag caaagtggcc ggcggctgtc cttccttcct gccgtcaata tgccttgaat
      481 aattctctat tcccgaacag aaccctatat tcctaatcat ctccaaatca taattggttc
      541 tctgattgag cctgtaagcc tccactatct tgccttgatt ttcaaaatat gccacccgct
      601 cttgcagttc cgctcgaata tcttctgtgg ctctcttaag ttttgtatct ttagaaacaa
      661 aatggcttgc gggaaaaacc gccagatgat tgacataacc tgttatctct ccggtcaatg
      721 aatcaaactg cgtaattttc tccacctcat ctccgaaaaa gcttatcctg tacccgacat
      781 ttgcactgtt ggaaggagca atttctaaag aatctccttt tgctctgaaa gttcctcttt
      841 taaaattcat ttcatttcgt gagtactgta tttccaccag tttctttatc acatcatccc
      901 tgtcaagtgt catgcccacc ccgacataca gcattaagtc ctcataatcg gcaggactgc
      961 ccagaccgta aatgcaggaa accgatgcta ctattattac atcccttctt tgcagaagag
     1021 ccgatgtggc ggcatgtcgc agctgatcta tttcgtcatt tatggacata tccttttcaa
     1081 tgtaggtatc ggtagaggga atatatgctt cgggctgata atagtcgtaa taggatacaa
     1141 agtattccac actgttatcg ggaaagaact ctttaaactc cgaacacagc tgggcagcca
     1201 atgtcttgtt atgtgcaatg acaagagtgg gcttctgaac tttttcaata atatttgcca
     1261 tggtaaaagt cttacccgaa ccggtaactc ccagaagagt ctgatgttta taccctgaaa
     1321 gcactccgtt tgccaatgtc tctattgctg caggctggtc acctgtaggt cggaaatccg
     1381 attgcaaaac aaatttatct gatctgaact ctttgtctct cttgctcatg taattatttt
     1441 agcacacaac attacgcttt atgctattat actgtataat gtataagtaa aattgtgtac
     1501 gcaaggagaa ttatggaaaa caaaagaaaa tcggtattct ggatatttat aaagagcttt
     1561 acggacatgc atgcatataa acactttgtg tataagaatt tttcaaaagc attcttatac
     1621 attctgctgt ttacttttct gctttccgtt attttctcgg ctatatttat agcatcaatt
     1681 aaagattaca ctacccaact tataacgcaa ctggaacaaa atactcctaa gtttaccatc
     1741 aaaaacggag aacttgtaat agagaatgat caaccggtaa ttcttgcgga aaccgatgat
     1801 aaaggaacgc ttataataat cgacaaagaa tatacgggat cggttaccaa gtatgaagaa
     1861 tatgatactg ttatagtttt aggacaaaag gaaatatatt tatccaattc ggctataagg
     1921 tacagtatag gatatgaatc ggttttaggg caaaaacagg aagttacttc ggaaatgttg
     1981 ttcggcagag tatcttcatc aaaacccttc ctttatctat tcttctttgc ctctatgttt
     2041 attttcctaa tcggtaacta ccttttctat actttgttcg tagcattaat agttaatacg
     2101 gtaaggcgta tacggagaaa gcaggcaaat ctgggtattt cattgcaact ctcagcctat
     2161 gccctcacat tacctgccat tatatatctt atctggattt tcacttcact tataactatc
     2221 cccatagaac ctcttatctt cgttcttgcg gtgattatag cttacaaggg agtagatgcc
     2281 tattacaaga gtatagatga ag
//
LOCUS       DAOPWC010000259         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_49822_length_2293_cov_2.769884, whole genome shotgun sequence.
ACCESSION   DAOPWC010000259 DAOPWC010000000
VERSION     DAOPWC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49822_length_2293_cov_2.769884"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1005
                     /locus_tag="PKJ65_05840"
     CDS             139..1005
                     /locus_tag="PKJ65_05840"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HNZ41389.1"
                     /translation="MIGYIKTYPPELKIKEYVKYNAYYCGICHSIRDRWGQVPRLTLN
                     YDCVFLAVFIDSIKKKENKYSDFRCIVHPVMKKKKVINSDTVDYIADINIYLSYMKLI
                     DNVKDEKNIFHLSSERFMRKYGKKLRKKYAELTDKIDKHLEDMATLEKEKCDSIDRVA
                     ECYAKIIEDVCLFAVKDIDENLKNIVGRIAYNVGNWVYTADAFDDIEKDIKKGMYNPY
                     LARFGYEKGRDIAEFKKEIKERCSFLLYAPLKAATDLYEDMDDELKDPIVKNIIYEGL
                     YDRTEKILNGEK"
     gene            1008..1571
                     /locus_tag="PKJ65_05845"
     CDS             1008..1571
                     /locus_tag="PKJ65_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNZ41390.1"
                     /translation="MDPYKVLGVNRNDSDETIKKAYRELVKKYHPDKYTDTDLKDLAN
                     EKLQQVNMAYDTIVKNRKSKDYGYSYDESSYKGSFAEVRRHILNKNYAAADNILRNSA
                     NRNAEWHYLRGVIFQKQGWYDQAYSYFEKAHLMEPSNAEYATAYSQTRSTANAYRSNV
                     YTRGYSSADEDFCRLCQCMLCASLCCR"
     gene            1585..2100
                     /locus_tag="PKJ65_05850"
     CDS             1585..2100
                     /locus_tag="PKJ65_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41391.1"
                     /translation="MREKTKELARSAILLAIMMILLYISTIIPGYKLAFYLLISLIPG
                     IVVIEYGIKQSIIFIIASFILSLVMPGDKITLLLFYSFFGIYAVIKYLIEKIRSRIII
                     LAVKLIYFIAVFFLNMYFAAKIAGILPEVMLKHNMWLVFGVAIIVFVIYDIMYTSFSQ
                     FYLNKIKKHVK"
BASE COUNT          840 a          335 c          473 g          645 t
ORIGIN      
        1 ctgtttagtt tgaacaaaaa cattatcgat atacttatga ttatagcggt ggttatgcag
       61 ataataacat taataggata tactacactg agtttgaaaa agattaaaga aataagcaag
      121 gaaaagaggg cggcatagat gataggatat attaaaactt atccgccgga attgaagata
      181 aaggaatatg taaagtataa tgcatattat tgcggaatat gccattctat cagagacaga
      241 tggggacagg ttccgagact tactttgaat tacgattgtg tatttttagc ggttttcata
      301 gactccatta aaaaaaagga aaataagtat tcggactttc gttgtattgt tcatcccgtt
      361 atgaagaaaa agaaggttat aaacagcgat acggtcgact atatagcgga cattaacata
      421 tacctttcct acatgaaact tattgataac gtaaaggatg aaaaaaatat ctttcacttg
      481 tcttccgaaa ggtttatgag aaagtacggg aaaaaattaa ggaaaaaata tgctgaactt
      541 acggataaaa tagataagca tttagaggat atggcaactt tagaaaagga aaaatgtgac
      601 agtatcgacc gggtagcgga atgttatgca aagattatcg aagatgtttg tctgtttgca
      661 gtaaaagata ttgatgagaa tctcaagaat attgtcggta gaatagcata caatgtaggc
      721 aactgggtat atacggctga tgcttttgat gatattgaaa aagatattaa aaaaggaatg
      781 tataatccct atcttgcacg tttcggatat gagaagggaa gagatatagc ggaatttaag
      841 aaagaaataa aggaaagatg ctcattttta ttgtatgcac cgttaaaagc cgcaaccgac
      901 ttatacgaag atatggatga tgaattaaag gatcctattg taaagaatat aatatatgaa
      961 ggtctttacg acaggacgga aaaaatattg aacggagaga aataaagttg gacccttaca
     1021 aagtcttagg tgttaatcgc aatgacagcg atgagacaat taaaaaagca tacagagagc
     1081 tggttaaaaa ataccatccc gataaatata ccgatacgga cctgaaggat cttgcaaacg
     1141 agaagctcca gcaggtaaat atggcatatg ataccatcgt aaagaacaga aaaagcaaag
     1201 attacggtta ttcatatgat gaaagcagct ataaaggcag ttttgcagag gtaagacgcc
     1261 atatacttaa taaaaattat gcggcggcgg ataacatctt acgtaattcc gcaaacagaa
     1321 atgccgagtg gcactatttg cgcggcgtta tattccagaa acagggatgg tatgatcagg
     1381 cgtattcata ctttgaaaaa gctcacctga tggagccttc caatgcggaa tatgcaacgg
     1441 catattcaca gacaagaagt acggcaaatg cctacagaag caatgtatac acaagaggat
     1501 acagttcagc ggatgaggac ttctgcagac tttgccaatg tatgctttgt gccagtttgt
     1561 gttgcagata aaggataaca gacaatgcgg gagaaaacca aagaacttgc aagatcggct
     1621 atactgcttg caataatgat gattttgtta tacataagca caataatacc gggatacaaa
     1681 ctggcttttt atttattgat aagtctgatt cccggaatag ttgttatcga gtacggcatt
     1741 aaacagagta tcattttcat aatcgcatca tttatacttt ctctggtaat gcccggagat
     1801 aaaataacat tgctgctgtt ttactcattt ttcggtatat atgccgtaat taaatatctt
     1861 attgaaaaaa tacggtcaag gattattata cttgcagtaa aactgatata cttcattgcg
     1921 gtatttttcc tgaacatgta tttcgccgca aagattgcgg gaatattgcc tgaagttatg
     1981 ctaaaacata atatgtggtt ggtttttggc gtagcaatta tcgtgtttgt aatatacgat
     2041 ataatgtata caagtttttc tcagttttac ttgaataaga taaaaaagca tgtaaaatga
     2101 acaccgttaa agttataaag tttgtatttt aagagaagaa atggaagaaa agtatttaaa
     2161 aaaaatagaa tttgacaaaa taatagcatt actatccgac tatgcggact cggcaccggg
     2221 taaatcaagt gttgaacaga tatctccttc aaccgattac gacaccataa tatccgatct
     2281 cagcctgctg aaa
//
LOCUS       DAOPWC010000260         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_50102_length_2284_cov_4.650516, whole genome shotgun sequence.
ACCESSION   DAOPWC010000260 DAOPWC010000000
VERSION     DAOPWC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50102_length_2284_cov_4.650516"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..788
                     /locus_tag="PKJ65_05855"
     CDS             126..788
                     /locus_tag="PKJ65_05855"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41392.1"
                     /translation="MIDIHSHILPKVDHGSSSLEESLRQLKTADRFGVSSIICTPHFY
                     PFKESIDRFLSRRDKAFSELSAANSTDIKLYKGAEVRLTTGLGNNKKIRKLTIENTNY
                     ILIEMPSSSWDNMLLNEIINLQSMGLTVIIAHIERYSPIQVQKLEGLDVLAQINAYSL
                     YSPLQALRIHRYNKKNMINFVASDTHIYNYLITYRALKRSRFFLGSLYDQFMVNASKL
                     II"
     gene            complement(781..1938)
                     /locus_tag="PKJ65_05860"
     CDS             complement(781..1938)
                     /locus_tag="PKJ65_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41393.1"
                     /translation="MRKTNALKLIIIILIMSAVLVSCTEEEYRASRLYRSLTSYEQKS
                     ETVTLKNKKYNKIDLSKKAVLELSNGMIYNVDFNEAVNVKEASTASILSSEIISKTLA
                     LKVADKDTVMNVISTDIASYGDYVISVTDNAVFTGSGLKVNNLGETGAAIKVSSGASM
                     VLNKSNVKAKGAGVESDSLVSISSSEMTVESLKFYEGATVTLDDSRFYTNRGIMLLDN
                     ANENLIHISLNLKKAKLTVADGAMFSMIDTKASVKIEDTTLDRSLSNILLLKNSEATV
                     TLCKSNAEGGIMTDDSSSLNLLIKNGSAFKGYINKGNRTKTVTVQIEEKSVWEVTSDS
                     YVRGLILKDANFENIKSNGFTVYYDSMNSTNAWLNKETINLPDGGRLVPFR"
     gene            2038..>2284
                     /locus_tag="PKJ65_05865"
     CDS             2038..>2284
                     /locus_tag="PKJ65_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ41394.1"
                     /translation="MDILGIKIDNIDMENALLKASDALSDNESFRYIFTPNAEICMAA
                     IKDSSLMEVLNSSFMNIPDGDGVVLASKILKTPLKEKV"
BASE COUNT          676 a          463 c          406 g          739 t
ORIGIN      
        1 taatgtggac actatgccct ataacaaaca gtacaggtac ggtaaaaacg ggtataagta
       61 tgcaaaatat ggcggatacg gtggatacga caataagcct gtgacgccac ctgtaaatct
      121 tgacaatgat tgacatacat tcccatatat taccaaaggt cgaccacggc tcttcctctt
      181 tggaagagtc tttaagacag ttaaaaactg ctgacaggtt cggtgtatct tctattatct
      241 gtactcctca tttttatcct tttaaagaga gcattgacag attcctttcc cgaagagata
      301 aggctttttc agagttatca gctgcaaata gtacagatat taagctttat aaaggagcgg
      361 aagtacggtt gacaacggga ctcggtaata ataagaagat ccgcaagctc acaatagaga
      421 atacaaatta catacttata gaaatgccct catcctcctg ggataatatg ctcctcaatg
      481 aaatcataaa tctgcagtct atggggctta cggtaataat agctcatata gaaagataca
      541 gtcccataca ggtacagaag cttgaaggac tggatgtgct tgctcagata aatgcatatt
      601 ccctttattc tcctcttcag gctttaagaa tacacagata taacaaaaag aacatgatta
      661 attttgtcgc cagcgatacg cacatatata attaccttat tacttacaga gctttaaaaa
      721 ggagccgctt ctttctgggc tccttatatg atcaattcat ggttaatgca tcaaaactca
      781 ttatctgaac ggaaccagtc ttcccccgtc aggaagattg attgtttctt tattcagcca
      841 tgcattggtt gaattcatgg aatcataata tactgtaaat ccgttggatt ttatattttc
      901 aaaatttgca tcctttaaga taagtcctct tacatagctg tccgatgtaa cttcccaaac
      961 gcttttttcc tctatctgga cagtaacggt ttttgtcctg tttcccttgt ttatatatcc
     1021 tttaaaagct gaaccgtttt taatgagcaa gttaagagaa gaactgtcat cggtcataat
     1081 gccgccttcc gcattggatt tgcacaaggt aaccgttgct tcggaattct tcaacaacag
     1141 tatattggat aaactccggt ctaatgttgt atcttcaatc ttaacggagg ctttagtatc
     1201 tatcatggaa aacattgccc cgtcggcgac ggtcagtttg gcttttttaa gattaagcga
     1261 aatatgaatc agattttcat tggcattatc caaaagcatt atgccgcgat ttgtgtaaaa
     1321 ccttgaatca tccagggtca ctgtcgctcc ttcgtaaaac ttaagactct ccaccgtcat
     1381 ttcgctggaa cttatactca ccagcgaatc ggattcaacg ccggctcctt ttgcctttac
     1441 attggatttg tttaatacca tggatgcacc cgaagaaacc tttattgccg ctccggtttc
     1501 tcccagattg tttactttaa gacctgaacc tgtaaataca gcattatcgg ttacacttat
     1561 tacataatcc ccgtatgatg caatatcggt agatattaca ttcataaccg tatctttatc
     1621 cgccactttt aaggcaagcg tttttgatat tatttccgaa ctcagaatgg aagcggttga
     1681 agcttcttta acattgaccg cttcattgaa atccacatta tatatcatac cgttggaaag
     1741 ttccagtacg gcttttttgg ataaatcaat tttattgtac ttcttgtttt ttaaggtaac
     1801 cgtttcgctt ttctgctcat atgaggttaa agatctgtac agcctggaag cgcggtactc
     1861 ttcttcggtg cacgaaacaa gtactgctga cataatcaat ataatgatga ttaactttaa
     1921 agcattggtt ttcctcatgt ctgcgaagtt tattctatat ccatatttat caataatcaa
     1981 cctttttatt gatttttcac cggtttttac ctatacttca aagtacttgg agagattatg
     2041 gacatcctgg gtataaaaat cgataacatt gatatggaaa acgcattgtt aaaagcatct
     2101 gatgctttgt ccgacaatga gtctttcaga tatatcttta ctcccaatgc ggaaatatgc
     2161 atggctgcaa ttaaagacag cagtcttatg gaggtattaa attcctcttt tatgaatata
     2221 cctgacggag acggagttgt tcttgcctca aaaattctta agacccctct aaaagagaaa
     2281 gttc
//
LOCUS       DAOPWC010000261         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_50458_length_2274_cov_2.240649, whole genome shotgun sequence.
ACCESSION   DAOPWC010000261 DAOPWC010000000
VERSION     DAOPWC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50458_length_2274_cov_2.240649"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKJ65_05870"
     CDS             <1..491
                     /locus_tag="PKJ65_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41395.1"
                     /translation="AVGQNDKKFCMGKTVYLVTTTEAHSDITALDCFVSKVAISGLTD
                     NELYKAARISLSVTDDLGVETTYIYRYAADGTDTSGAALPVNGINTKSSTDPSYAMGI
                     YESGQAGALPFKLICANSGTISVRELTIRIWFEGENNYAVRAHAGGGFAFELEFTLVD
                     PA"
     gene            504..1082
                     /locus_tag="PKJ65_05875"
     CDS             504..1082
                     /locus_tag="PKJ65_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41396.1"
                     /translation="MKKKKIFKVLYMCISTVFIASAIITTVMILSFDMQYACPKFGNH
                     ILVSVNDSELSPKILKGSALVSDLKYDKVENGDYVIITTKSMAGIQYSVRHLESVEDE
                     YYLVSGTEGRGPFYVNKSSVMAVSKMQIYGLGGYINMMRTRNGFLLLVIIPLVIVIII
                     QVIRMLLLIQEKKLKEQYANVTADETVNREEM"
     gene            1082..1831
                     /locus_tag="PKJ65_05880"
     CDS             1082..1831
                     /locus_tag="PKJ65_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41397.1"
                     /translation="MPKNIIKKGIIAFSALLLLIIACLFRFGSISTAWFREDRKSGMT
                     ASNIYSITQCETSSVAPFKNDEVAFDADCTLDDIMGNYEKTIIPGDVLYYAFLILDVD
                     IVSYQTFMMEFAGLLVLEGEGGFNSESFASNLCIEENTVIFAVLEATPDGMGGETYEL
                     YPYDNGNAFYSITNTGVDHIYTEENGLQADLQFTLPEGVAYPAGTNGKADILVIVPVW
                     YMDTGANQNIEMNCSLTIEGCVLYPFTGGGQ"
     gene            1828..>2274
                     /locus_tag="PKJ65_05885"
     CDS             1828..>2274
                     /locus_tag="PKJ65_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41398.1"
                     /translation="MKEFVKNSKGILLTALSIVLLIAILVTASLAWFYFPTAESVTVE
                     SDIQHEIDVDLYQYSESLGYFVKAGLNAAEDTVVATCGTEATFVEWGGIFYTTNDITD
                     YYMLMFTYPDDDFLNGNLEAYVGMMLQSGMNGTEANYKIVYTTIEYA"
BASE COUNT          703 a          384 c          549 g          638 t
ORIGIN      
        1 ccgctgtcgg acagaacgat aagaaatttt gcatgggaaa aaccgtttat ttggttacta
       61 caacggaagc acatagtgat ataacggctc ttgactgttt tgtcagcaaa gttgcaatat
      121 ccggtcttac cgacaatgaa ctttataaag cggcaaggat atcactttcg gtgacggatg
      181 atcttggtgt agaaacaaca tatatataca gatatgccgc agacggtacc gatacctccg
      241 gtgctgcatt gccggtaaac ggcataaata caaaaagttc gaccgatccg tcgtatgcga
      301 tgggaatata tgagagcgga caagcgggag cattgccgtt taaactcata tgtgcgaata
      361 gcggtacaat cagcgtaaga gaactgacaa taagaatttg gtttgaaggt gagaataatt
      421 atgctgtgag agcacatgcg ggagggggat ttgcatttga actggagttc acacttgtag
      481 atcccgcata aggagattaa ttgatgaaaa aaaagaaaat tttcaaagtt ttatatatgt
      541 gcatatcgac agttttcatt gcttccgcaa taataacaac cgttatgatt ttaagctttg
      601 atatgcaata tgcatgtccc aaattcggta accatatatt ggttagcgtc aatgattctg
      661 agctttcccc taagattcta aaaggcagcg ctttagtgtc cgatctgaaa tacgacaagg
      721 tggagaatgg cgattatgtg ataataacca ccaagagcat ggcaggaata caatattcgg
      781 tcagacatct ggaaagtgtg gaggatgaat attatctggt gtcgggaacc gaaggcagag
      841 gtccgtttta tgtaaataaa agcagtgtaa tggcggtgag caaaatgcag atatacggtc
      901 tcggagggta tataaacatg atgaggaccc ggaacggttt cctgctttta gttattattc
      961 ctctggtaat tgtaataatc attcaggtta taaggatgtt attgctcata caggaaaaga
     1021 agttgaaaga gcaatatgcc aatgtgaccg ccgatgaaac cgtaaacagg gaggagatgt
     1081 aatgcctaag aatatcatta aaaaaggcat aatcgcattt tccgctttat tgctgcttat
     1141 tatagcatgc ttattccggt tcggcagtat ttccaccgca tggttcagag aagacagaaa
     1201 atccggtatg acggcatcca atatatattc gattacccaa tgtgagacaa gcagcgttgc
     1261 tccattcaag aatgatgaag ttgcatttga tgccgactgc actctggatg atattatggg
     1321 aaattatgaa aaaacaataa ttcccggtga tgtactttat tatgcatttc ttatcttaga
     1381 tgtcgatatc gtgtcatatc aaacctttat gatggagttt gcgggactgt tggtgttgga
     1441 aggtgaagga ggtttcaatt ccgaatcttt tgcaagcaat ttgtgtattg aggaaaacac
     1501 cgttatattt gccgtgttgg aagcaacgcc ggacggtatg ggaggggaga cctatgagtt
     1561 atacccgtat gataatggaa atgcctttta cagtataacc aataccggag tcgaccatat
     1621 ttacacggaa gaaaacgggt tgcaggcgga ccttcaattt acattaccgg aaggtgtggc
     1681 atatcccgca ggaactaacg gtaaagcgga catattggta attgtacctg tctggtatat
     1741 ggataccgga gctaatcaga atatagagat gaactgttcg ttaacaatag aaggatgcgt
     1801 attgtatccg tttacggggg gcgggcaatg aaagaatttg tgaaaaacag taaagggata
     1861 ctgttaaccg ctttaagtat agttttgctt atagcgatac ttgtcactgc cagtttagca
     1921 tggttctatt tcccgactgc cgagagtgtg acagttgaat cggatattca acacgagata
     1981 gatgtggatt tatatcaata cagtgaaagt ttaggttact ttgttaaggc gggactgaat
     2041 gctgcggaag ataccgttgt agcaacctgc ggaacggaag caacttttgt tgaatggggc
     2101 ggaatatttt ataccactaa cgacattacg gactactata tgcttatgtt tacatatccc
     2161 gacgacgatt ttttgaacgg aaatcttgaa gcatatgtag gtatgatgct ccaatcgggt
     2221 atgaacggga ctgaagccaa ttacaagatt gtatatacca ctatagagta tgca
//
LOCUS       DAOPWC010000262         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_50902_length_2262_cov_2.894427, whole genome shotgun sequence.
ACCESSION   DAOPWC010000262 DAOPWC010000000
VERSION     DAOPWC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50902_length_2262_cov_2.894427"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..590)
                     /locus_tag="PKJ65_05890"
     CDS             complement(399..590)
                     /locus_tag="PKJ65_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41399.1"
                     /translation="MGEYYRSDDDIEKLQRKETASTVTEQVSDKKDYDFIGDLLEDSE
                     TLVKGMVLSEILGKPKAKR"
     gene            complement(593..1591)
                     /gene="floA"
                     /locus_tag="PKJ65_05895"
     CDS             complement(593..1591)
                     /gene="floA"
                     /locus_tag="PKJ65_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778175.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNZ41400.1"
                     /translation="MEPLIIVLIIVAVIIAFFSVLLSLVPIGLWISAIAAGVKVSLMS
                     LIGMKFRRVQPAKIVNPLIKSTKAGLNVTINQLETHYLAGGNVNKVADALIAAQKARI
                     QLSFETAAAIDLAGRDVLEAVKMSVNPRVIETPIISAVAKDGIEVKAKTKITVRANMS
                     RLIGGAGEQTIIARVGEGIVTSVGSSESYKDVLENPDLISQTVLKKGLDAGTAFEILS
                     IDIADVDIGRNIGAQLQTDQAEADKKIAQAKAEERRVMAVAREQEMKANIEEMRAKVV
                     EAEALIPKAMAKAFEEGNLGVLDYYNMRNVMADTKMRESISEAGKAAKPQAAKKDK"
     gene            complement(1591..>2262)
                     /locus_tag="PKJ65_05900"
     CDS             complement(1591..>2262)
                     /locus_tag="PKJ65_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ41401.1"
                     /translation="GYGIGFQMDSGNTINRCIYLFHEPGRLGFEEVAKIIPVKRERYD
                     IINTEGEIKMPGIFSFVENIDILQAILLVVGMILLIIEAFIPSFGVVGIIGIVLLFLS
                     IFLTAKTFLEGVIMFLILLAIAVLLLVIVVRIATKSRLSKKLINTDTFSEEQGYLGVE
                     DMSAKVGLKGKAVTILRPAGKAVFEGEILDVVTKGEFVEEGKGVIIEQVEGNRIVVKE
                     IKESV"
BASE COUNT          591 a          565 c          344 g          762 t
ORIGIN      
        1 cgcatgttct ccagtaagag cgaacatatt taacccgtta atcttaatgt aatcttttaa
       61 gcgttctgca tattccttat ccgacaactt gatatatagt cttatcttac ccgaattatt
      121 catattagct cctatcgagc catattatac cattatttac catttttgcg cattcaacat
      181 atgacaaaca catgtaacat agtacatatc ggcaatatca ttctttagtt atgaccaagt
      241 ccacaataaa aattgctgtc acattctata gtccttcatt caaaagttat tgctatcttt
      301 catgaactcc aacaagttaa cgttgcagat tttgactttt catcaattcc tgcaacgcta
      361 agtcgggttt atcaacgatt cttttgaata tttcaaatct atctctttgc cttaggttta
      421 cctaatatct cacttaaaac catgcctttt accagggttt cgctatcttc cagtaaatcc
      481 cctataaaat cataatcctt tttgtccgaa acctgttccg taaccgttga agcggtttct
      541 ttccgctgca acttttctat atcgtcatct gacctgtaat actcacccat tgctatttat
      601 ctttcttagc ggcttgaggt ttagcggctt ttccggcttc ggatatggat tccctcattt
      661 tggtatccgc cattacattt ctcatattgt aatagtctaa aactcccaag ttgccttctt
      721 caaaagcttt agccatggct ttaggaataa gagcttcggc ttccacaact tttgccctca
      781 tctcctcaat gttggccttc atctcctgct ctcttgctac agccattacc cttctttcct
      841 cggctttggc ttgcgctatc ttcttgtccg cttccgcctg atcggtctgc agttgagcac
      901 cgatatttct tccgatatcc acatcggcta tatcaataga cagaatctca aatgcagtac
      961 ccgcatccag tccttttttc agaacagtct gggaaataag atcgggattt tccaatacat
     1021 ccttgtagga ttcggatgag cctacggaag ttactattcc ttcacctact cttgctatga
     1081 tagtctgctc tcccgctcct cctatcaatc tggacatatt tgcacggacg gtgatttttg
     1141 ttttggcttt tacttcaata ccgtcttttg caacagcgga tattataggg gtttcaataa
     1201 ctctggggtt aacactcatt tttaccgctt ccaaaacatc tctgcccgcc agatcaatag
     1261 ctgccgccgt ttcaaaagac aactgtatgc gtgctttctg tgcagcaatc aaagcatcgg
     1321 caaccttgtt tacatttccg ccggcaaggt aatgagtctc cagttgattt atcgttacat
     1381 tcagccccgc tttggtggat ttgataaggg gatttacaat cttagccggc tgaacccttc
     1441 gaaatttcat tccgataagg ctcatcagac ttactttaac tcctgcagct atcgccgata
     1501 tccatagtcc gataggtacc aggcttaaca acactgaaaa gaatgctatt attactgcta
     1561 cgattattaa tactataatt aacggttcca ttaaacactc tcctttattt cttttactac
     1621 tattctgtta ccttcaacct gctctattat tactcctttt ccttcttcta caaactcacc
     1681 tttagtgaca acatccaaga tttctccttc aaacaccgct ttccctgcag gccttaatat
     1741 tgtgaccgct tttcctttaa gacccacctt ggcgctcatg tcctctaccc ccaggtaacc
     1801 ttgctcttcg gaaaaagtgt cggtattgat aagttttttg gaaagcctgc tttttgttgc
     1861 aattcttact actataacca aaagtagcac cgctattgcc agaaggataa gaaacattat
     1921 aacaccttcc agaaaagtct ttgctgtaag aaaaattgac agaaaaagca acactattcc
     1981 tataataccg accacaccga aactcggtat aaaagcctct atgataagca gtatcatgcc
     2041 tacaaccagc aagatggctt gcagaatatc gatgttttct acaaaactga aaatacccgg
     2101 catttttatc tctccttccg tgttaattat atcataccgt tctctcttta cgggtattat
     2161 ctttgcaact tcttcaaatc cgagccgtcc gggttcatgg aataaatata tacatcggtt
     2221 gattgtgttt ccggaatcca tttgaaatcc aataccataa cc
//
LOCUS       DAOPWC010000263         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_50971_length_2260_cov_2.257143, whole genome shotgun sequence.
ACCESSION   DAOPWC010000263 DAOPWC010000000
VERSION     DAOPWC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50971_length_2260_cov_2.257143"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKJ65_05905"
     CDS             <1..380
                     /locus_tag="PKJ65_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41402.1"
                     /translation="TPVATAPNIDTWYDIALNGENVKYSSLEDPYIKYQARKYGISNT
                     IGIWYTGISWYQGPKSQTPTRKQIIDKALEINCREWWGAFAPSTTPPPRGKYIWPTEL
                     YNNYDYYLKKLSQLKTSYYKQKK"
     gene            387..1907
                     /locus_tag="PKJ65_05910"
     CDS             387..1907
                     /locus_tag="PKJ65_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ41403.1"
                     /translation="MNLARMILKNTLSNWTGMAVATAITILITPYILTQLGQERYGIY
                     NIVFAVMQYLVLLEFGIRGAVARFASKYIQAKDSTALSSVISLAILFSLIIGLIAIGL
                     SVLIGYAALDFFHVSSLYQTQTLILFVASGLNLMLSFLCYSLSGVLIGSNRYELQNLQ
                     IIINNVVRAALIVVFFESGWISLTSWAVAIIVATLASLMYLFVIVFHVQPGLRIGLKN
                     LRPANMKEMFHFGGWNIFLQLSGLLTASANPILIGRLLGSAMVPFYSIPFLLVSRLQE
                     GVSGMTSTLMPHASSTLNTGDKAFLAHLLKRGTYTASLLVFPLGGCLLIMCRSLFRVW
                     LPPQYEDYWVIYAIFMIAYFGSITQTSSYYVLLGGGNIKWMSFVYVGAGFCIVALCLL
                     FVGVFKMGIEGAAIALVIPRFISTCLFQPWFAARQSDLKVFEYLTKAYIRPVLYSIPS
                     LLLAFCFSMYMPARSLPVWIAEYLLALLPTTVFIILEFDTFGLREISQKILKRKLI"
     gene            1904..>2260
                     /locus_tag="PKJ65_05915"
     CDS             1904..>2260
                     /locus_tag="PKJ65_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41404.1"
                     /translation="MKFYLENGGHHKGNKGDMAILISLIREFEEIWPGSTYFIPLPDP
                     GVDVSSKPQLHFIIPLEDYVIRTGQSNGRWRIAWRMFKTILKTYSLKICKHHLKNGSL
                     DRCLASMMESDAMVLTG"
BASE COUNT          574 a          473 c          507 g          706 t
ORIGIN      
        1 gtactccggt tgctacagcc cccaacatag atacatggta tgatattgct ttaaacggag
       61 aaaatgttaa atattcgtcc ctggaagatc cttatattaa gtatcaagcc cgaaaatacg
      121 gaatatccaa taccattggg atatggtaca ccgggattag ctggtaccaa ggcccaaaat
      181 ctcaaacgcc gacacggaag caaatcatcg ataaggcttt agaaataaat tgccgcgaat
      241 ggtggggagc attcgcacca tccactactc ctccacccag aggaaaatat atctggccca
      301 ccgaactcta taacaactat gactattacc tcaaaaaatt aagccagttg aaaacttctt
      361 attataagca aaaaaagtaa gtgcacatga acttggcaag aatgatatta aagaataccc
      421 tttctaactg gacgggaatg gccgtcgcta ccgccattac catcctcatt acaccctata
      481 tccttactca gttagggcag gagcggtatg gaatctataa tattgttttt gccgtgatgc
      541 agtatctggt tcttcttgag tttggaattc gtggggcagt ggcaagattt gcaagtaaat
      601 acattcaggc gaaggattcc accgccctga gcagtgttat ttcgttggcg attcttttct
      661 cactgattat aggtctgatt gccatcggtt tgagtgtact gatcggttat gcggctttgg
      721 attttttcca tgtttcatct ctttaccaga ctcagacatt aattttattt gtggcttcgg
      781 gcctaaattt gatgctgtct tttttatgtt attccctttc gggcgttctt attggttcca
      841 atcggtacga gctgcagaat ctgcaaatta tcataaacaa tgtagtgcga gctgcactca
      901 ttgttgtatt ttttgagtcg ggttggatca gtttgacaag ttgggcagta gcgattattg
      961 tggctacgct agccagcttg atgtatctgt ttgtcattgt atttcatgtt cagccgggat
     1021 tgcgaatcgg ccttaaaaat ctgcggccag ccaatatgaa agaaatgttt cactttggcg
     1081 gttggaatat ttttcttcag ctctccggcc tcctgaccgc gtccgccaat ccgatactca
     1141 tcggacgact actgggttct gccatggtgc cgttttattc cattcctttt ttgctggtgt
     1201 caagattaca agaaggcgta agcggaatga catcgactct gatgccgcat gcctcctcaa
     1261 cgctgaatac cggtgataag gcatttctgg ctcatttgtt gaaacgagga acctatacgg
     1321 cctccctgct ggtttttccg ctcggagggt gcctgctgat tatgtgcaga tcccttttcc
     1381 gggtatggct gccgcctcaa tatgaagatt attgggtgat atatgcgatt tttatgatcg
     1441 cctattttgg ttccattaca cagacttcct cctattatgt cctgcttggc ggcgggaata
     1501 ttaagtggat gtcgtttgta tatgttgggg cagggttttg cattgtggct ctttgtctgc
     1561 tttttgtcgg tgtctttaag atgggaattg aaggagcagc gatcgccctg gttattccca
     1621 gattcatctc aacttgtctt tttcaaccct ggttcgcagc taggcagagc gatttaaaag
     1681 tatttgagta tttgacaaag gcttatatac ggccggtctt gtattcgatt cctagtttac
     1741 ttctggcatt ctgtttcagc atgtatatgc ctgccagaag ccttcccgta tggattgcag
     1801 agtatttgct agcattgctg ccaactacag tttttatcat actggaattt gatacttttg
     1861 gcctcaggga aatctctcaa aaaatattga aacgcaaact gatatgaaat tttatctgga
     1921 aaacggtggt catcataaag gcaacaaggg ggatatggca atactgattt ctttgattcg
     1981 ggagtttgaa gaaatatggc ccggcagtac ctatttcatt cctctgccgg atcccggcgt
     2041 tgatgtttcc agtaaaccgc aattacattt tataatacct ttggaagact atgttataag
     2101 aaccggacaa tcgaatggac gctggcgtat tgcatggcga atgttcaaaa ctattttgaa
     2161 aacttacagc ttgaaaatat gcaaacatca tctcaagaac ggatccctgg atcgatgtct
     2221 ggcaagcatg atggaatcag atgccatggt cctgacggga
//
LOCUS       DAOPWC010000264         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_51026_length_2258_cov_3.221516, whole genome shotgun sequence.
ACCESSION   DAOPWC010000264 DAOPWC010000000
VERSION     DAOPWC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51026_length_2258_cov_3.221516"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKJ65_05920"
     CDS             <1..470
                     /locus_tag="PKJ65_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNZ41405.1"
                     /translation="TDPVLWRGPVISGVLKQFYTDVVWGELDYLLIDMPPGTADTALT
                     VFQSIPVDQAIIVTTPQDLVSMIVEKAIKMADMMHISVLGAVQNFSYAVCDKCNNKIE
                     LFGNTDTNKLSIPVLDRIPFDKRLSAAMDRGMIELTEDTYIANTVKKLIEKSK"
     gene            complement(597..824)
                     /locus_tag="PKJ65_05925"
     CDS             complement(597..824)
                     /locus_tag="PKJ65_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNZ41406.1"
                     /translation="MKNKIDIKFLSISDVKEFADIVSAFDYDIDLVSGRYVVDAKSIM
                     GIFSLDLSKPISLVAHTDDIKPLTDAIKRFC"
     gene            910..1779
                     /locus_tag="PKJ65_05930"
     CDS             910..1779
                     /locus_tag="PKJ65_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNZ41407.1"
                     /translation="MIKLKNRHLTVIINPLGAEIVSVIKDGYEYLWQGNPDIWKGQAP
                     NLFPFIGRLKDEEYEYDNRLFKVKIHGFARHSLFEIVESNENYVSLRITGTPDTYAVY
                     PFDFDFIVSYELMDNIIKKQYMIINNGYKVMYYEVGGHEGYNLTFEKNKIMDDYYILF
                     SDDLIYNYTTDKNIMINKQTEEINLSDRKLYLKPEIFKNDALILDCSKLSNREVVLEC
                     STSSRKVKVRFEDFDYLGIWTRPFYTNYVCIEPWSALPDCNFIDKKLENKIGIRKLTA
                     KKTDMLEYFIEVI"
     gene            1776..>2258
                     /locus_tag="PKJ65_05935"
     CDS             1776..>2258
                     /locus_tag="PKJ65_05935"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNZ41408.1"
                     /translation="MMIDNLVKEIKKAKSIVFFTGAGVSTESGIPDFRSTKGLYLSKY
                     DGYTPEVIISHSFFYHNPDVFYRFYHDKIVHPEAKPNDFHYGITKLKDMNYDVSVVTQ
                     NIDDLHNIAGNGKVYELHGSISRNNCIKCHKFFNLDYIIANRGKTVRCDECNSLVKPD
                     V"
BASE COUNT          757 a          358 c          459 g          684 t
ORIGIN      
        1 cgacagatcc tgttttgtgg aggggtccgg ttatttccgg tgtgcttaaa caattttata
       61 ccgatgttgt atggggagaa ttggattatc tgctcataga tatgcccccc ggaacggcag
      121 ataccgctct tacggtattc cagtccatac ctgtagacca agccataata gttacaacac
      181 cgcaggatct tgtatccatg atagtggaaa aagcaattaa aatggctgat atgatgcata
      241 tttcggtttt gggagccgta cagaatttca gttatgcggt atgcgataaa tgtaataata
      301 aaattgagct tttcggtaat acggatacaa ataaactgag tattccggtt ctggacagga
      361 taccttttga taaaagatta agtgctgcaa tggacagagg aatgatagag cttacggaag
      421 acacatatat cgccaatacg gtaaagaaac ttattgaaaa gagtaagtaa gttttatata
      481 tcaatacaga tttattaaaa aataccggaa attgatatta ataatcagtg ggatatcagt
      541 taaatatcag taaaatcaaa ttttctgcaa aaaaaaacgg ggtaacccgt ttgtttttaa
      601 caaaatctct ttatagcgtc tgttaacggc ttaatatcat cggtatgtgc aaccaatgat
      661 atgggtttac ttaggtctaa actgaatata cccataatgg atttggcatc cacaacatat
      721 ctgccggaaa ccagatcgat atcgtaatca aaagcggaga ctatatccgc aaattccttg
      781 acatcgctta ttgaaagaaa ttttatatca attttgtttt tcatgtcatc ctcctaactg
      841 cttcaatata tcatatagtt actgaaacat caataacggt tgtgatataa tattatctgt
      901 gaggcagata tgattaaact taaaaacaga catttaaccg ttattatcaa tccgttagga
      961 gcggaaatag tttccgttat caaggacgga tatgaatatc tatggcaggg aaatcccgat
     1021 atctggaaag gtcaggctcc caatctgttt ccttttatag gaagactgaa agatgaggaa
     1081 tatgagtatg ataacagact gtttaaagta aagatacacg gatttgccag acattcgctt
     1141 tttgagattg tggagagcaa tgagaattat gtatcgttga ggattaccgg cactcccgat
     1201 acttatgccg tatatccgtt tgatttcgac tttatagttt cctacgaact gatggataac
     1261 ataatcaaga agcaatacat gataattaat aacgggtata aggtaatgta ttatgaagtc
     1321 gggggacatg aagggtataa tctgacattt gaaaagaata aaataatgga cgactactac
     1381 attttgttct cagacgattt aatatataat tacacaaccg ataagaatat tatgattaat
     1441 aagcaaacgg aagagataaa tctttccgat aggaaactgt atcttaagcc tgagattttt
     1501 aaaaatgatg cccttattct ggattgttca aaattgagca acagggaagt tgttctggaa
     1561 tgctctactt cgagcagaaa agtaaaagta cgatttgaag attttgatta tctgggtata
     1621 tggacccgcc ctttttacac aaattatgtg tgcatcgaac cgtggtccgc tctgcctgat
     1681 tgtaatttta ttgataaaaa gctggagaac aagataggta tacgtaagtt aactgccaag
     1741 aagacggaca tgttggagta ttttattgag gtgatatgat gattgataat ttggttaagg
     1801 aaattaaaaa agcaaagagt atagtttttt tcaccggtgc aggtgtgtct accgaaagcg
     1861 gtataccgga ttttcgaagt accaaaggac tttacttaag caaatatgac ggctatactc
     1921 ccgaggttat tataagtcac agttttttct atcataatcc cgatgtgttt tatcgcttct
     1981 atcatgacaa gattgtgcat cctgaagcaa agcccaatga ttttcattac ggtataacaa
     2041 agcttaaaga catgaactat gatgtgtccg ttgttacaca gaatattgac gatttacaca
     2101 atatcgccgg taacggtaag gtttacgaat tgcacggaag tatcagcagg aataattgta
     2161 tcaagtgcca taagtttttc aatctggact atataatagc caacagaggt aaaacagtca
     2221 gatgcgatga gtgtaattca ttggtaaaac ccgatgtg
//
LOCUS       DAOPWC010000265         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_51431_length_2247_cov_1.830292, whole genome shotgun sequence.
ACCESSION   DAOPWC010000265 DAOPWC010000000
VERSION     DAOPWC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51431_length_2247_cov_1.830292"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKJ65_05940"
     CDS             complement(<1..495)
                     /locus_tag="PKJ65_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41409.1"
                     /translation="MKYEDIKNEISSNIFKQVYLFYGDDEYLKQSVVNALKTAIIRYT
                     QAEYDNVKFDDTSKIEDIINECATYSFSGNNKFITCRNTGFFNKEEFSDKIISLSDYV
                     TEGVYLFFIENDINKSLSGFKHYTSKNSAYEITKGTAEDIKRFVYSAFRKEGKNISSE
                     NLNLF"
     gene            complement(515..>2247)
                     /locus_tag="PKJ65_05945"
     CDS             complement(515..>2247)
                     /locus_tag="PKJ65_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ41410.1"
                     /translation="RVMKHLNEDKRGIVTALIAGLRIYLDRNTTSTFQKSGLSHILSI
                     SGTHFNILIIPFYYLLTSISKNNRFSMIFTIFAVLFLLVFTGLRISAIRAALCIVTLF
                     FSNMLYFDSNRYLPICLSSLIIILINPLSVYDYGFIMSYCCVVSIALFSRKIRSFFVL
                     QIDKLKKTLQEKVLKKHIVLEDYYRRIRKTKLKAVILKVLSAVSLLLSVQPFTVFITY
                     RLFHCIYPYSLFSNLIIFILITPLLLSLWTAILFPRIMCPLFGLLADFLIKFGEYIGN
                     LPYSKLYVKHIPDIFFIIVICTFLYIKYKPVKSRINKYIVISVAMLLCVITVYINNTP
                     KVIFMDVGQGDCAVITLSNGYNIMIDTGEYIDLSAVAYRTGHKIDMIILTHSDSDHCG
                     AIEDVLDNFSVDNVYLPYTNHKDNIKLSDQLSMNYPDTYRGMLKRGDEIRTEGVCIKV
                     LNPDTDIVYTNANDGSVVFNLYTKAGSFLFTGDADIKKIPSDIFLKCDIIKMPHHGDD
                     KAINSEIIHYISPKIAVISVGKHNRYGHPGRKTLDTLTEKGINTLRTDINGSVTVYLI
                     DRKYYLFKYR"
BASE COUNT          721 a          383 c          377 g          766 t
ORIGIN      
        1 aaaaagattg agattttccg aagatatgtt cttcccctct tttctgaaag cggaatatac
       61 aaatctctta atgtcctcag cagttccttt ggttatctcg taagcactgt ttttagaggt
      121 atagtgctta aagcccgaca gacttttatt aatgtcgttt tcaatgaaga ataagtagac
      181 accttcggta acataatcgc taagactgat aattttatca ctgaattcct ctttgttgaa
      241 aaatccggta ttcctgcagg ttatgaattt attgttaccg ctgaaggaat aagtggcaca
      301 ttcgttaatg atatcctcta tttttgaagt atcatcaaac tttacattgt catattccgc
      361 ttgcgtgtat cggataattg ctgtttttaa agcatttacc accgattgct taagatactc
      421 gtcgtctccg taaaacagat acacctgctt aaaaatgttg ctcgatattt catttttaat
      481 atcttcgtat ttcacacata gattttaaca catttcatct gtatttaaac agatagtatt
      541 ttctgtctat aagatatacg gtaaccgatc cgttaatatc cgttcgcaag gtatttatac
      601 ctttttccgt aagtgtatcc agagtttttc ttccgggatg gccgtacctg ttatgttttc
      661 cgaccgatat aaccgctatt ttcggagata tataatgaat tatttccgaa tttatagcct
      721 tatcatctcc gtgatggggc attttgatta tgtcacattt taaaaatata tctgaaggta
      781 tcttttttat gtccgcatct ccggtaaaaa gaaaactgcc ggcttttgta tacagattaa
      841 aaactaccga accgtcattt gcatttgtat aaacgatatc agtatcgggg tttagtactt
      901 tgatacacac tccttcggtt cttatctcat cacctctttt gagcataccg cgatatgtat
      961 ccggataatt catgctcaat tggtcagata gtttaatatt gtccttatga tttgtatatg
     1021 gaagataaac attatccacc gagaagttat ccaaaacatc ttcaattgca ccgcaatgat
     1081 cagaatcgga atgtgtaagt ataatcatat ctattttatg tcctgtacga tatgcaacag
     1141 cggataaatc tatatattcg cctgtatcaa tcattatgtt atatccgttt gacaaggtta
     1201 taacggcgca atcaccctgc cctacatcca taaaaatgac tttcggagta ttatttatat
     1261 aaacagttat tacgcataat agcattgcaa cactgataac gatatattta tttatcctgc
     1321 tttttacagg tttatacttt atatacaaaa atgtgcatat aacaatgata aagaaaatat
     1381 cgggtatatg tttaacatag agctttgaat aaggtaaatt gccgatatat tcgccaaatt
     1441 taataagaaa atctgccaac aacccgaata gggggcacat tatccgggga aataatatag
     1501 cggtccaaag cgataaaagt aacggagtta tgagtataaa gattataaga ttggaaaaga
     1561 gagagtaagg atatatacag tgaaacaacc tgtatgttat aaacaccgta aaaggttgaa
     1621 cggataatag aagacttact gcggatagta cttttagaat aactgctttc aactttgtct
     1681 tccttattct tctgtaatag tcttccaata cgatatgttt cttcagcacc ttttcctgca
     1741 gggttttctt taatttatca atctgtaata caaaaaatga acggattttt ctgctgaaca
     1801 gtgctattga taccacacag cagtaggaca taataaagcc gtaatcgtaa acagataaag
     1861 gatttatcaa aataatgatt aatgaagaga gacatatagg cagatacctg ttggagtcga
     1921 aatagagcat attggagaaa aataatgtaa caatacataa tgccgctctt attgcactta
     1981 ttcttaaacc tgtaaataca agtaaaaata atactgcaaa tattgtgaat atcatagaga
     2041 atctgttgtt ctttgatatt gaagttaaca gataataaaa cggaatgatt aatatgttga
     2101 aatgggtgcc ggatatgctt aagatgtgcg ataatccgct tttttggaat gtacttgttg
     2161 tgtttctgtc cagataaata cgtaaaccgg ctataagggc tgtaactatt cctcttttat
     2221 cttcattaag atgtttcatt acccttt
//
LOCUS       DAOPWC010000266         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_51636_length_2240_cov_3.784897, whole genome shotgun sequence.
ACCESSION   DAOPWC010000266 DAOPWC010000000
VERSION     DAOPWC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51636_length_2240_cov_3.784897"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="hisS"
                     /locus_tag="PKJ65_05950"
     CDS             <1..868
                     /gene="hisS"
                     /locus_tag="PKJ65_05950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNZ41411.1"
                     /translation="GSDSCNADIEVLFMLYSLFTELGVQGLHLNINSIGCKTCRSGYN
                     KILKEYFSDKPDNMCADCKRRYITNPMRLLDCKVEKCAEIAKDAPMLLDNICNQCKDH
                     FYNIIAGLDEINIPYIINKRLVRGLDYYVRTVFEFIAEGIGAQDTVCAGGRYDGLVEQ
                     LGGTDTPGIGFAMGLERLLETMKISGFKFPTAKTPVIFIGYIGEKAYKAARTIANELR
                     LKGIYCEIDINDRSLKAQLKFADKKDALYSVVLGDVEIDTNKAIIKNMKTRETKEVSV
                     DSIVRRVVENII"
     gene            877..>2240
                     /gene="aspS"
                     /locus_tag="PKJ65_05955"
     CDS             877..>2240
                     /gene="aspS"
                     /locus_tag="PKJ65_05955"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ41412.1"
                     /translation="MENIKGLKRTGYCAEYDIKDVGKKAVLMGWVNRSRNLGSIIFCD
                     IRDRTGLIQVVFREERDKSLFKKAINLKAEYVIAVTGIVNEREDKNPNIKTGDIEIEV
                     EELRILSESAVPAIDIAENTATNELMRLKYRYLDLRKPKQQHYLIMRNKIAKCTRYFF
                     SENGFIEVETPVLTRSTPEGARDYLVPSRVNQGKFYALPQSPQLLKQLLMISGLDRYY
                     QIVKCFRDEDLRANRQPEFTQIDLEMSFVDVDDVIAVNERFLKKLFKETSDIDLSIPF
                     PRMTYEEAMNRFGSDKPDLRYGMELTDISKLVKDSEFKVFSDACSNNGSVRGINAKGC
                     ADFSRKDIDKLTEYVKDYRAKGLAWLKVGANNEITGSITKFFTDSQQKAIVDAFEGQE
                     NDLILIIADKNNVVFDALGALRVEIARRLNLADDERQEFLWVTEFPLLEFDEETNRFY
                     AKHHP"
BASE COUNT          783 a          334 c          527 g          596 t
ORIGIN      
        1 aggttccgat tcatgtaatg cggatattga agtattattc atgctttact cattatttac
       61 cgaattgggt gttcagggtt tacatcttaa tatcaacagt atcggctgta aaacatgcag
      121 atcgggatat aataagatac taaaggaata tttttccgat aagccggata atatgtgtgc
      181 ggactgtaaa agaagataca taaccaatcc gatgaggttg ttggattgta aagtggagaa
      241 atgtgccgag attgccaaag acgctccgat gcttctggat aatatctgca atcaatgtaa
      301 agatcatttt tataatataa tagcaggtct tgatgagata aatattccgt atattattaa
      361 taaaaggctt gtcagaggac tggattatta tgtcaggacg gtgtttgagt ttattgcgga
      421 aggaataggt gcacaggata ccgtctgtgc gggcggaaga tatgacggcc ttgttgaaca
      481 actgggcggt accgatactc ccggaatagg ttttgccatg gggctggagc gacttctgga
      541 aaccatgaaa atttccggtt ttaagtttcc gactgcaaaa acacctgtta tttttatcgg
      601 ctatatcggc gaaaaggcat acaaagcagc aagaactatt gcgaatgaac tgaggcttaa
      661 aggaatatac tgcgaaattg atattaatga cagaagttta aaagctcagc tgaaatttgc
      721 cgataagaag gatgctttat acagtgttgt actgggtgac gtggaaatag atacaaataa
      781 agcaattatt aagaatatga aaacacggga aacaaaagaa gtcagtgttg attccattgt
      841 aagaagagtt gtggaaaata taatatgagg tatggtatgg agaacataaa aggcttaaaa
      901 agaaccggat attgtgcgga atacgatatt aaagatgtag gcaaaaaagc ggttctgatg
      961 ggatgggtta acagatcaag aaacttgggc agcattatat tctgcgatat tagagacaga
     1021 accggtttaa ttcaggttgt tttcagagaa gaaagagata agtctctttt taaaaaggca
     1081 atcaatctga aagctgaata tgtaatagcc gtaacaggta tcgtaaacga gcgtgaagat
     1141 aaaaacccca atataaagac gggagatata gaaattgagg tcgaagaatt aaggatactg
     1201 tcggaaagtg ccgttccggc aatagatatt gcggaaaata ccgcaacaaa tgaattgatg
     1261 cgattaaagt accgttatct ggatttaaga aaacccaaac aacagcacta cctaataatg
     1321 agaaacaaga ttgcaaaatg caccaggtat ttcttttcgg agaacggatt tattgaagtg
     1381 gaaacaccgg ttttaaccag aagtactccg gaaggtgcca gagattatct tgtacccagc
     1441 agggtgaatc aggggaaatt ctatgcatta cctcaatcac ctcagctttt aaaacagctt
     1501 cttatgattt ccggactgga cagatattat cagatagtta aatgtttccg tgacgaagat
     1561 ttaagagcaa acagacagcc ggaatttaca cagatagacc tggaaatgag tttcgtggat
     1621 gtagacgacg taatagcggt caacgagcgg tttttaaaga aattgtttaa agaaacatcg
     1681 gatattgacc tttcgatacc ttttcccaga atgacctatg aagaagcaat gaacagattc
     1741 ggcagcgata aacccgattt gagatacggc atggaattaa ccgatatttc aaaattggta
     1801 aaagattcgg agtttaaagt attttccgat gcatgtagta ataacggatc ggtaagggga
     1861 ataaatgcaa aaggctgtgc cgatttctcc aggaaagaca tagataaatt aacagagtac
     1921 gttaaagatt acagagcaaa aggactggca tggctgaaag taggagcaaa taatgaaatt
     1981 acgggttcca taacaaaatt ctttaccgac agtcagcaaa aagcaatagt tgatgcattt
     2041 gaaggtcaag aaaacgattt gatattgata atagcggata agaacaatgt ggtttttgat
     2101 gctttaggtg cattacgtgt ggagattgca aggagactga atcttgcaga tgacgaaaga
     2161 caggaatttc tgtgggttac ggagtttccg ttattggagt ttgacgagga aacaaacaga
     2221 ttttatgcaa agcatcaccc
//
LOCUS       DAOPWC010000267         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52090_length_2227_cov_3.593923, whole genome shotgun sequence.
ACCESSION   DAOPWC010000267 DAOPWC010000000
VERSION     DAOPWC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52090_length_2227_cov_3.593923"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKJ65_05960"
     CDS             <1..428
                     /locus_tag="PKJ65_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNZ41413.1"
                     /translation="SVLPENLILEIAESGFADNLEMINNQLTKAQILGIRFAIDDFGT
                     GYSSLAKEHEIAADIIKIDKFFIDQLLIKDEKELITSDIINLAHKMGQKTVAECVEHP
                     RQLEYLKRNNCDYYQGFLFSKPVKAKKAIKLLEAGKFAV"
     gene            complement(444..1589)
                     /locus_tag="PKJ65_05965"
     CDS             complement(444..1589)
                     /locus_tag="PKJ65_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ41414.1"
                     /translation="MNSYERVMAAFSKEKSDHVPIYCGSVSSRVASQVMGRTMYVGGG
                     IQQYRESLARFRGEEAHREFAEKSRQDAFDWNEAMDFDYVRPSYWRYRYKPTKMLDDV
                     TFYYEREDGTHWIMKYFDEYELFACMEDSKKDVDDPDLLEEEVINTEQYNKRYVPDIS
                     MHSDYIDTMSYFNNRRATYTGGLALLIPNTREVWFEAIAARPDLVERYLEALTQRALM
                     NIPLIASIGARIAYGGGDCAGNRGLFYSNEVFKKLMVPRLRRISDKCHEYGLYHVFGS
                     DGYFWDVADDFYIHSGIDGHYEADCSCGMDIRSVRTKYPDITVIGGISAATLDSKSQD
                     DVRTEVMEAVLAAKELNGAIVGCSNLVSPTTPIKNFMLMMELLHKYK"
     gene            complement(1699..>2227)
                     /locus_tag="PKJ65_05970"
     CDS             complement(1699..>2227)
                     /locus_tag="PKJ65_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNZ41415.1"
                     /translation="REVFIPRSVTYISPTAFSQCPEFAKVEVEQGNKVYKSFNGVLFK
                     DGMSEIVYFPVSFMRTEYYIAHGTRIIGEKAFYKSPLYTVHIPDTVTYIQDYAFAESS
                     DMKSVTLPANLIYLGENVFLGNGKLRTITIGAGVEIQPNSVDGISFYEQYQLYGAGTY
                     LKTGNNIWEYIHDKD"
BASE COUNT          616 a          529 c          408 g          674 t
ORIGIN      
        1 attccgtttt gccggaaaac ctcattctgg agattgccga atcgggtttt gccgataatc
       61 tggaaatgat aaacaatcaa cttacaaaag cccagatttt aggcatacgg tttgccatag
      121 atgatttcgg tacaggttac tcctcattgg caaaggaaca tgaaattgcc gccgatataa
      181 tcaagataga caaattcttc atagaccagc ttcttattaa agatgaaaaa gaactcataa
      241 cttcagatat tataaatctt gcccataaga tgggacaaaa aacagttgcc gaatgtgttg
      301 aacatccgcg acagttggaa tacctaaaga gaaacaactg tgactattat caaggctttc
      361 ttttctccaa gccggtaaaa gcaaaaaaag ccattaagct tcttgaagcc ggaaagttcg
      421 ctgtttaaca gagaaattca gagtcatttg tacttatgaa gaagctccat catcagcatg
      481 aagtttttta tcggagtagt gggcgaaaca agattggaac atcccactat tgcaccgttc
      541 aactcctttg cagccaaaac ggcttccata acttccgttc tgacatcgtc ttggctttta
      601 ctgtctaaag tcgctgccga tattccccct attaccgtta tatcgggata ttttgtcctt
      661 accgagcgga tgtccattcc gcaggagcag tcggcttcat aatgtccgtc tataccgctg
      721 tggatataaa aatcatccgc cacatcccag aaataaccgt ccgacccgaa gacatgatac
      781 aacccgtatt catggcattt gtccgatatt ctcctcagac ggggaaccat cagcttctta
      841 aatacttcat ttgaataaaa cagtcctctg tttccggcac agtctccccc tccgtatgcg
      901 atgcgagcac ctatggaagc tatcagcggt atattcatca atgctctttg tgtcaaagcc
      961 tccagatatc tctccaccaa atccggtcgc gccgcaatcg cctcaaacca tacttctctt
     1021 gtattgggta tcagaagagc cagaccgccg gtataggtag ctctgcgatt gttgaagtaa
     1081 ctcattgtat ctatataatc gctgtgcatg gaaatatcgg gaacatatct tttattgtat
     1141 tgctcggtgt tgattacttc ctcttccaga agatcgggat cgtctacatc ttttttacta
     1201 tcttccatgc atgcaaaaag ctcatattca tcaaaatact tcattatcca gtgtgtaccg
     1261 tcttctcttt cataatagaa tgtaacatcg tccagcattt tggtcggctt gtagcgatat
     1321 cgccagtagg aagggcgcac atagtcaaaa tccatcgctt cattccagtc aaaagcatcc
     1381 tgcctggatt tttctgcaaa ttccctgtgg gcttcttctc cgcggaacct tgcaaggctt
     1441 tctctgtact gctgaatacc gccgcccaca tacatggttc tccccattac ctgtgatgcg
     1501 actctcgacg aaacggaacc gcaatagata ggtacatgat cgcttttttc tttagaaaat
     1561 gccgccatta ctctttcata cgaattcata atgctttata tatacagtca gcaactgttt
     1621 ctccctctcc ttctttttaa ataaaatgac accgatacaa attgattata tccgtcaaca
     1681 gcggtcgttt aatatcattc agtctttgtc gtgtatatac tcccatatat tattgccggt
     1741 cttcaaatat gtgcccgctc cgtaaagttg atactgttcg taaaaggaaa tgccgtctac
     1801 ggaattgggc tgaatttcta caccggcacc tattgtaata gtgcgcagtt tgccgtttcc
     1861 cagaaataca ttctctccga gatatataag attggcaggc agagttacac ttttcatatc
     1921 gctgctctcc gcaaaagcat aatcttgtat atatgttaca gtatccggta tatgaaccgt
     1981 gtaaagcggg cttttgtaaa aagccttttc tccgattatt cttgttccgt gtgctatata
     2041 gtattccgtt ctcataaacg aaacggggaa atacactatt tccgacatgc cgtccttgaa
     2101 cagtacgccg ttaaatgact tatatacttt gttaccttgt tccacctcta cttttgcaaa
     2161 ttccggacac tggctaaaag ccgtcggact tatataagta accgatctag ggataaatac
     2221 ttctctt
//
LOCUS       DAOPWC010000268         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52139_length_2226_cov_2.877476, whole genome shotgun sequence.
ACCESSION   DAOPWC010000268 DAOPWC010000000
VERSION     DAOPWC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52139_length_2226_cov_2.877476"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1401)
                     /locus_tag="PKJ65_05975"
     CDS             complement(142..1401)
                     /locus_tag="PKJ65_05975"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNZ41416.1"
                     /translation="MYSTEKAYKQAKEYYKRFGIDTEKAMKLCDKVPISIHCWQGDDV
                     GGFEKSASDLTGGLQVTGNYPGKPRTADELRSDLDVVMKYLPGVKKINLHAIYQEAEE
                     FVDRDAIKPEHFKKWADWAVDRNIGLDFNPTYFSHPLSENGTLSAANDEVREFWIRHG
                     IACRRIGQYFYERTGKPCVVNHWTSDGSKEIPHDTLSPRLRLKDSYDRIFAETADVKG
                     VIDGIESKLFGIGLESYTAGSFEFCLSYVINKRKNHIILTMDTGHYHPTEVVSAKLGA
                     IFAFQKSILLHVSRPVRWDSDHVVSFDDETRAIFEELVKLGKLNKTYVATDYFDASIN
                     RVMAWVVGLRNTRKAILAALLQPNEMLSKVATRNDNTGVLAIREEFKSAPFGLVWDYY
                     CEKAGAKSGIEWINDIKQYEQDVLLKR"
     gene            complement(1473..>2226)
                     /locus_tag="PKJ65_05980"
     CDS             complement(1473..>2226)
                     /locus_tag="PKJ65_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ41417.1"
                     /translation="EIHSVYLVDEPELILSAKMAKETDTLIHIHLHETKVEVENCIKT
                     YGMTPLQLCAKTGVLDNRVTAAHTVHVNDEDMLLMKEKNVIPVHNPSSNLLLGSGIAD
                     IPKMLKSGIKPAIGTDGAASNNTLDMFKEMHLTSLIHRGCKEDSNVINARQVLTMATA
                     NGARALGFDNCGMIKTGMKADIILVNTESLNMTPFHDPVSALVYSARATDVDTVMVNG
                     EILMKDKQLLTVDEEKVKYEAAGSAARLYNLF"
BASE COUNT          556 a          526 c          387 g          757 t
ORIGIN      
        1 tcaccgttac tgttacggca tttctttaat actatgtaat acattgcttt atataaaata
       61 tgctgaaaca aaaaaccggg tataccggtt tttttgttct atgtactctt ttcttcttaa
      121 tacttcttta gcagttaaat actatctctt taaaagaaca tcctgctcgt actgtttgat
      181 atcattgatc cattcaatac ccgatttggc accggctttt tcacagtagt aatcccaaac
      241 caaaccgaac ggagcggatt tgaactcttc tctgattgcc aatacaccgg tgttatcatt
      301 tcttgttgca accttggaaa gcatttcgtt aggttgtaac agagcggcaa gtatggcttt
      361 tctggtattt cttaagccca caacccatgc cattaccctg ttgatggaag catcgaaata
      421 gtcggttgct acataagttt tattaagttt gcccaatttt accagttctt caaatatagc
      481 tctggtctca tcatcaaaag aaactacatg gtcggaatcc catcttacag gtctgctgac
      541 atgtaagagt atcgactttt ggaaagcgaa tatcgctccc agttttgccg ataccacctc
      601 ggtgggatga taatgaccgg tatccattgt aagaatgatg tgattctttc tcttgttaat
      661 aacatagctc agacagaatt caaaactacc tgcggtataa ctttccaatc ctattccgaa
      721 caatttactc tctataccgt ctatgacacc ttttacatcg gctgtttccg caaatattct
      781 gtcataactg tcttttaatc tcaatctcgg tgacagagta tcatgaggta tttctttgga
      841 accgtcgctc gtccagtggt ttacaacgca gggtttccct gttctttcat agaaatactg
      901 acctattctt ctgcaggcta ttccgtgtct tatccagaat tcccttacct catcattggc
      961 agccgacagc gtgccgtttt cgctcaaagg atgagaaaaa tatgtgggat taaaatccag
     1021 tccgatattt ctgtccacag cccagtccgc ccatttctta aaatgctcgg gttttatggc
     1081 atctctgtct acaaattcct ccgcttcctg ataaattgca tgcagattta tttttttgac
     1141 tcccggaaga tatttcatta caacatccag gtctgaccgt aattcatcgg cggtcctcgg
     1201 tttgccagga taattacccg ttacctgaag tcctccggtt aaatccgatg cactcttttc
     1261 aaatccgccc acatcatcgc cttgccagca atgtattgaa atcggaacct tgtcacataa
     1321 tttcattgct ttttccgtgt ctattccgaa acgtttgtaa tactccttag cctgtttata
     1381 tgctttttcc gtactgtaca tattcaccct cctgtttttc tttattctat catttttaca
     1441 aagcattttc agcattatcc ggtatttttc tgtcagaata agttatatag tcttgctgcc
     1501 gacccggctg cttcatattt taccttctct tcatcaactg tcagcaactg cttatccttc
     1561 ataagaatct caccgtttac catcacggta tccacatcgg ttgctcttgc tgaatagacc
     1621 aatgccgata ccggatcatg gaacggtgtc atgttaaggc tttcggtatt gaccagtata
     1681 atgtcggctt tcataccggt ttttatcata ccgcagttat caaatcccaa tgctcttgca
     1741 ccgttggccg ttgccatagt cagcacctgc cgtgcattta taacattact gtcttcttta
     1801 cagcctctgt gtataagtga agtgagatgc atctctttga acatatccaa agtgttattg
     1861 cttgccgcac cgtctgtgcc tattgcaggt ttaatgcctg atttaagcat tttgggaatg
     1921 tccgcaatac cgctgccgag caaaagatta cttgacggat tgtgtaccgg tatgacattc
     1981 ttttctttca tcaacagcat atcttcatca tttacatgaa cggtgtgtgc ggcggtaacc
     2041 ctgttatcca gaacaccggt cttggcacat aactgtaaag gagtcatacc gtatgttttg
     2101 atacagtttt ccacctcaac ctttgtttca tgcagatgaa tatgtataag ggtatcggtt
     2161 tccttagcca tttttgcact taagattaat tcaggctcat ctaccagata caccgaatgt
     2221 atttcc
//
LOCUS       DAOPWC010000269         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52205_length_2224_cov_2.835408, whole genome shotgun sequence.
ACCESSION   DAOPWC010000269 DAOPWC010000000
VERSION     DAOPWC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52205_length_2224_cov_2.835408"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKJ65_05985"
     CDS             complement(<1..536)
                     /locus_tag="PKJ65_05985"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNZ41418.1"
                     /translation="MKKYNLAIVGATGMVGRTFIKVLEERKLPIKNIYFFASARSCGN
                     TITFSGKEHVILELNEHSFDRLIDIALFSAGSDLSKIYAPVAAQKGVVVIDNSSAFRM
                     DPTVPLVIPEVNKEDIAKHNGIIANPNCSTIQALVAVKPLHDAYKVKRMVVSTYQSVS
                     GAGKAGCDDLENCLKGLPP"
     regulatory      complement(606..782)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            951..1892
                     /locus_tag="PKJ65_05990"
     CDS             951..1892
                     /locus_tag="PKJ65_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ41419.1"
                     /translation="MGFEIILSIVIFVVVILFILSFIAGLFMFNITMIPRSPRNYFNK
                     KEEDIDERIKEFFASSEQWVKENTTSVWSLTSFDNLNLKAVFVENKGSHKWVILVHGY
                     TSRGWHMAQFAKQYYETGFNILMPDLRGHGDSEGQYATMGYYDSVDIIGWISQIIKKD
                     SDAKIVLHGISMGAATVMLTTGEDLSNHVKCAIEDCGYTSVWDIFSYNMKRFFKLPAF
                     PVLYVSNIISRARVRMDFKQASPLNAVKKSKTPTLFIHGDADQLVPFYMLEPLYDNAA
                     CEKDILVVKGAEHGASSAKEPDVYHKKVSEFISKYLN"
     gene            1962..>2224
                     /locus_tag="PKJ65_05995"
     CDS             1962..>2224
                     /locus_tag="PKJ65_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41420.1"
                     /translation="MEKHVQKIHDFYNFKKDAAIFQKEFGFYVKGKWKEQGWINDDTD
                     TDKLFGFDKPAVFFLGRCGWCEAPLVPSFERKILSTTDECEIE"
BASE COUNT          660 a          428 c          479 g          657 t
ORIGIN      
        1 gggggaagcc cttttaagca attctccaaa tcgtcacatc ccgctttgcc cgcaccggat
       61 accgactgat atgtggaaac caccattctt ttcactttat aggcgtcatg taacggtttt
      121 accgcaacca aagcctgaat ggtggagcag ttgggattgg ctataatgcc gttatgcttt
      181 gcaatatcct ctttatttac ctcgggtata accagaggaa ccgtcggatc cattctgaac
      241 gcactgctgt tgtctattac aaccacgcct ttctgtgccg caacgggagc atatatttta
      301 cttaaatcac tgcctgccga gaacagggca atatcaatca atctgtcgaa tgaatgctca
      361 ttcaactcca aaatgacatg ctctttaccg gaaaaagtaa tggtattgcc acaagaccgc
      421 gccgaagcaa agaaatagat attcttaata ggaagttttc tttcttctaa aacctttata
      481 aaggttcttc ccaccatacc tgtggcgcct actattgcca agttatattt tttcattttt
      541 ttcacccgcc tgctgtcata ttatatcaaa aaaaggctgc ctgtgcgagc ctttacttaa
      601 accttccaag tgacagctct ccaccgcaaa ttgacggtga cagtcggtcc gttattgaca
      661 aaaccgacca gacaaacgca tacaaggctg cgtattcttc ggcggttgcc cctttttccg
      721 tcatcatccc gccttgtacg gtcagaacgg atacttatga ctcccgcgcc tctatcaata
      781 ttaccggaag tataacaggc gttaaggata atgtaaaggt gtttttatca cactaaaaat
      841 aaatatcggg attataggtt aaattgtatt gtatacagag ttaaatttgc ataaatctga
      901 tagatgagag tgatgatgtg atataatttt aatgtatgac ggagggggaa atgggttttg
      961 agattatttt atcaattgta atatttgttg ttgtcatttt gtttatcctg tcgtttatag
     1021 cgggtttgtt tatgtttaat ataaccatga tacccaggtc gcctaggaat tatttcaata
     1081 aaaaagaaga agatattgat gagcggataa aagagttttt cgcatcaagt gaacagtggg
     1141 taaaggagaa cactacttcg gtgtggagtc ttacttcttt cgataatctt aatcttaaag
     1201 ccgtatttgt tgagaataag ggtagtcata aatgggtaat attggttcac ggatatacat
     1261 cacgcggatg gcatatggca cagtttgcaa agcaatacta tgagaccgga ttcaatatat
     1321 taatgcccga tttaagggga cacggcgaca gtgaagggca gtatgccaca atgggctatt
     1381 atgacagtgt cgatataatc ggttggataa gtcagattat taaaaaagat tccgatgcaa
     1441 aaattgtttt acacggaata tccatgggag ccgctaccgt tatgcttacg acgggggaag
     1501 atttgagcaa tcatgttaaa tgcgcaatag aggactgcgg ttatacatcg gtatgggata
     1561 ttttcagcta taatatgaaa aggttcttca aactgccggc ttttcccgta ctttatgttt
     1621 cgaatattat ttcccgggcc agagtcagga tggattttaa gcaggcaagc cctttaaatg
     1681 ccgttaaaaa gtccaaaaca cccactttat ttatacacgg tgatgccgat caactggtcc
     1741 cgttctatat gctggaaccg ctttatgaca atgcggcctg tgagaaagac atattggttg
     1801 taaaaggagc ggaacacggt gcgtcttctg caaaagagcc ggatgtgtat cataaaaaag
     1861 tatcggaatt tatatccaaa tacctgaatt aaagtcttaa taagtattta cttaactgtg
     1921 ctataatact atctgttatg tacttcaagc ggggagacaa tatggaaaag cacgttcaga
     1981 agattcatga cttctacaac ttcaaaaaag acgccgccat ctttcagaaa gagttcggtt
     2041 tttatgtcaa gggtaaatgg aaagagcaag gctggataaa tgacgacacc gataccgata
     2101 aattgttcgg atttgacaag cccgccgtat tctttttagg ccgttgcggg tggtgtgaag
     2161 caccgttggt tccttccttt gaaagaaaga ttctgagtac tacagatgaa tgtgagatag
     2221 agca
//
LOCUS       DAOPWC010000270         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52294_length_2221_cov_4.284857, whole genome shotgun sequence.
ACCESSION   DAOPWC010000270 DAOPWC010000000
VERSION     DAOPWC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52294_length_2221_cov_4.284857"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="PKJ65_06000"
     CDS             <1..1997
                     /locus_tag="PKJ65_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41421.1"
                     /translation="SNYHSLLQFTNDPDSIGSMIYTDIINGDVDTLLSEFMVDVGDSL
                     SKNMKLTRSETHFISRLKTELMQMFAITNLDCDIIILCICIQEMERTFCRIDNYPPHV
                     LIADMLKTSYSDVRSALSSDSALIRTGMIEDKTRHSSLFSVSTLVSDYILGMRKTIID
                     ETILETSDKEILPLENFIIENKSTTMMKDILKNDGPAHIFLYGKPGTGKTEISRTLSS
                     LSGKKCYRTSLGLNSNDAFSGRQNERLTALIAAQSIAARNGAVLIVDEADNILNTRSF
                     FNTQTTEKGWLNSFMDRTEAKIIWIANNISSIDPSTMRRFTYSLYFPDMSIPERAHVL
                     RYQLEQSRLAGILSDESIELIATKYEVNAGGFTSAVKTAAACCSDIPQQSDELENILC
                     EALEKHEILIRGEKRRNGKRILNQIASQYDPTVLNTDFPLDRIEHVVTMYGQSVRLLG
                     ESRGDGNVSMLFHGVPGTGKTEYAKYLAKQAGLHFIVKRASDLMNPFVGMTEKLIAAA
                     FAEADRSGAILFIDEADSLFQSRDKASHTWETTQVNEILTQMENFRGILICCTNLVTQ
                     FDIAAMRRFSFKVEFKPLNPEMRIALYKKYFISVCGDLTDIDRKRLISLESLTPGDLR
                     TVYRNATIIPDDNTTHAGIIDQLSKELILKKTVCEKKIGF"
BASE COUNT          690 a          503 c          440 g          588 t
ORIGIN      
        1 cctccaatta tcattctctt ttacagttta caaatgaccc ggattccata ggaagcatga
       61 tatatactga tatcattaac ggagatgtcg atacattact ttcagagttt atggttgatg
      121 ttggagactc tttatcgaaa aacatgaaac ttacgcgaag cgaaactcat tttatttcgc
      181 ggctaaaaac cgaattgatg cagatgtttg cgatcactaa tctcgactgt gatataatta
      241 tactgtgtat ctgcatacag gaaatggaaa gaacgttctg ccgtattgat aattacccgc
      301 ctcatgtact aatagcggat atgcttaaaa cttcatattc cgatgtccgc tctgcccttt
      361 cctcagattc cgcgctgatc cgtacgggaa tgattgaaga taaaacacgg cacagcagtt
      421 tgttttcagt aagcacgctc gttagtgatt atatacttgg gatgcgtaaa actatcatcg
      481 atgaaacaat tcttgaaaca tcagacaaag agattcttcc tcttgaaaat ttcatcatag
      541 aaaataaaag cacaacgatg atgaaagaca tacttaaaaa tgacggtcct gcgcatatct
      601 tcctttacgg caagccagga acagggaaaa ccgaaatatc acgaacactc tcttctttaa
      661 gcggaaaaaa atgttatcgt acaagcctcg ggctcaattc aaatgacgct ttctcgggaa
      721 gacagaatga aagattaacc gcattgatcg ctgcacagtc aatcgctgcc cgcaacggcg
      781 cggtactcat tgtcgacgaa gcagacaaca ttctaaatac acgcagtttc ttcaatacac
      841 aaacgaccga aaaaggttgg cttaattcat tcatggatcg cactgaagct aaaataatct
      901 ggattgcaaa caatatatct tcaatagatc cgtcaacgat gcgccgtttc acatacagtc
      961 tttattttcc tgatatgtct ataccagaac gggctcatgt tctccgctac cagctggagc
     1021 agtcgcgtct cgcgggaata ctttccgatg aatcaatcga actgattgca actaaatatg
     1081 aagtcaatgc cggaggcttt acttctgctg ttaagaccgc cgctgcctgc tgttctgaca
     1141 taccgcagca gtctgacgaa cttgagaata tcctctgtga agcgctcgaa aaacacgaaa
     1201 tcctcattcg cggagagaaa cgcaggaatg gaaaacgtat actgaatcag atagcttctc
     1261 aatatgatcc gaccgtactg aatacagatt tccctcttga tcgtatagaa catgtagtca
     1321 caatgtacgg tcagagcgtt cgtcttctag gagaatctcg cggtgacgga aacgtatcca
     1381 tgcttttcca tggtgttccc ggtactggca aaaccgagta tgccaaatat ctcgcgaaac
     1441 aggcgggact gcattttatc gtcaaacgcg catctgatct catgaatccg tttgtcggta
     1501 tgacggaaaa acttatagca gctgcatttg ccgaagctga ccgctccggc gctatactgt
     1561 tcatcgacga ggccgattca ctttttcagt cccgtgacaa ggcatcgcac acatgggaaa
     1621 caactcaggt gaacgaaatc cttacccaga tggagaactt ccgcggcata ctcatctgct
     1681 gtacaaatct tgttacacag ttcgacatcg ccgctatgcg ccgcttttct ttcaaggttg
     1741 agttcaagcc gctcaatcct gaaatgcgaa tagccctata caaaaaatat tttataagtg
     1801 tctgcggaga tctaacagac atcgacagga agcgtctcat atcgctcgaa tcactaacgc
     1861 cgggagatct caggacagtt tatcgcaacg cgacgatcat ccctgatgat aacacgaccc
     1921 atgccggaat tatcgaccag ctgtccaaag aactgatact caaaaaaacg gtttgtgaaa
     1981 aaaagatcgg tttttaactg aacagaagga atggtatagc tcatatatca ttccttctat
     2041 tataaaaagt cctcagctca attaaaaagg ttttaaaaaa acgcttacca gcttatcatt
     2101 tcaagcatac tctccggcac aaaaaaaata ttcattaaac ataaagagtt cgttagagat
     2161 ttatgcgctg tgttctgaaa ttaaaaggga acttaacaaa tagagttgcc gttcatgaag
     2221 g
//
LOCUS       DAOPWC010000271         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52379_length_2219_cov_3.735675, whole genome shotgun sequence.
ACCESSION   DAOPWC010000271 DAOPWC010000000
VERSION     DAOPWC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52379_length_2219_cov_3.735675"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..546)
                     /locus_tag="PKJ65_06005"
     CDS             complement(31..546)
                     /locus_tag="PKJ65_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ41422.1"
                     /translation="MIKIHTPFNDEVLCKLKPFDKVLITGYMYIARDQAHKKMYMEGI
                     PFDAAGQCIYHCGPSESKNNEVIGSAGPTSSYRMNEYMPYLFENGIRAFIGKGKVSDA
                     VVESIKRNRCVYFGAIGGLGAKLSKCIKKQRIISYESLGTEALREIYVENFPAVVIIN
                     CEGESIYERDG"
     gene            complement(543..1385)
                     /locus_tag="PKJ65_06010"
     CDS             complement(543..1385)
                     /locus_tag="PKJ65_06010"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNZ41423.1"
                     /translation="MTRKINSDIIIDSVAKVCFEAACVLDKQAYKAIETSLLKEDNET
                     AKYVLNELLDNANIAKRDCIPLCQDTGMVISFVEMGIKVQLDEPVEDLINKGIKKGYE
                     EGVLRKSVVKDAFQRINTDDNTPAIVYIKQTEGDKLKINLMLKGFGSENMSFVKMLNP
                     SLSQKEVVDIISDEICNRAYNACPPLFIGVGIGGTMDYACVLSKKALLRSLNESNINP
                     VYSDMESKILSNINKSDIGPAGYGGKTTALSVAILAYPTHIAGLPVAVTIQCHALRHR
                     EIIL"
     gene            complement(1369..1656)
                     /locus_tag="PKJ65_06015"
     CDS             complement(1369..1656)
                     /locus_tag="PKJ65_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ41424.1"
                     /translation="MMNRNELSIRLAQERNITKVQSDEIINTLFEIIRGALKDEGKVC
                     IKDFGTFLVKKRKGRKVKAIGSENVIAIPDIMELKLRVSKSMKDYLNDQED"
     gene            complement(1668..>2219)
                     /locus_tag="PKJ65_06020"
     CDS             complement(1668..>2219)
                     /locus_tag="PKJ65_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ41425.1"
                     /translation="RILIDPFCGTGTILIEAAMILKNIAPGLYRDFVSERWPVFKDNE
                     WSKVRQFYKSVIKEDNNVCLYGSDIDSSVIAAARHNARVAKVSDVINFSVKDFKDITV
                     PSEKGVIICNPPYGERLEDQAAASKIYADMSKKFKEFDNWSIYILAPEKVFEDAYGKK
                     ADKRRKLYNGKIICNLYQYFGAK"
BASE COUNT          597 a          489 c          340 g          793 t
ORIGIN      
        1 atgggtttcg gcatctttat tttttccata ttatccgtct ctttcgtaaa tcgattctcc
       61 ttcgcagttt attataacaa cagcggggaa attttcaaca tagatttctc tcagagcttc
      121 cgtacctaaa gattcataag aaataattct ttgtttctta atgcacttgg atagttttgc
      181 acccagaccg cctatagctc cgaagtatac gcatctgttt ctctttatgc tttcaacaac
      241 cgcatcactt actttacctt tgcctataaa tgctcttatt ccgttctcaa acagataggg
      301 catatattca ttcatccggt aactggatgt gggacctgcc gaaccgataa cttcattatt
      361 ctttgattcc gacggtccgc aatgataaat acattgtccc gcagcatcaa aaggtattcc
      421 ttccatatac atttttttat gagcctgatc tctggcaata tacatatatc ctgtaataag
      481 aaccttatcg aaaggtttta atttgcataa tacttcatca ttaaatggtg tatgtatttt
      541 tatcataata ttatttctct gtgtcttaaa gcatgacact gtattgtaac ggcaacaggt
      601 aatcccgcaa tatgagtagg ataagccaaa attgcgacag aaagagcggt ggtctttccg
      661 ccataccccg ccggaccgat atccgatttg ttaatattgc tcaaaatttt actctccata
      721 tcggaataaa cgggattaat attgctctcg tttaacgatc ttaacagcgc tttcttggac
      781 agtacacagg cataatccat tgtaccgcct ataccgacac ctatgaataa gggcggacag
      841 gcgttgtatg ctctgttgca tatttcatcg gatattatat ctacaacttc tttttgtgaa
      901 agtgaaggat taagcatttt tacaaaactc atgttctcgc ttccgaaacc tttcagcata
      961 aggttaatct ttaacttatc gccttctgtc tgtttgatat agacaattgc cggcgtattg
     1021 tcgtctgtat taatcctttg gaaagcatct ttaacaaccg acttccttag caccccttct
     1081 tcatagcctt tctttatccc tttgttaatc aaatcttcta caggctcatc aagttgtact
     1141 ttaatgccca tttccacgaa ggatataacc ataccggtgt cctggcaaag aggaatacag
     1201 tctctttttg ctatatttgc attatccagc agttcattta agacatattt tgcggtttca
     1261 ttatcttcct tcaaaagaga tgtttcaata gccttatatg cctgtttatc caatacacag
     1321 gcagcttcaa aacatacttt tgcaacagag tcgattatta tatcggaatt aatcttcctg
     1381 gtcattcaaa taatccttca ttgatttgga aactctgagt tttaattcca ttatatcggg
     1441 aattgcaatg acattttccg accctattgc ttttactttc ctgccttttc tcttttttac
     1501 aagaaaggta ccgaaatctt ttatacatac ttttccttcg tcttttaaag ctcccctgat
     1561 gatttcgaac aatgtgttaa taatttcatc actttgaacc tttgtgatat ttctttcctg
     1621 tgccaatctg attgataatt catttctgtt catcatgttc ccccaaacta ttttgctccg
     1681 aaatattgat atagattaca aatgattttt ccgttgtaca attttcttct tttatcagct
     1741 tttttaccgt atgcatcctc aaataccttt tcaggtgcaa gtatgtatat gctccagttg
     1801 tcgaattctt taaatttctt actcatatcc gcatatattt tacttgccgc cgcttgatct
     1861 tccagccttt caccgtaagg cggattgcag attatgacac ctttttccga tggaacggta
     1921 atatccttaa aatcttttac ggaaaagtta ataacatccg atacctttgc cacacgagca
     1981 ttatgtcttg ccgcagctat taccgagctg tcgatgtccg aaccgtacag acagacatta
     2041 ttatcttctt taattacgga cttgtaaaac tgacgtactt tgctccattc gttatcctta
     2101 aaaacaggcc atctttccga aacaaagtct ctgtataaac cgggcgcaat attctttaaa
     2161 atcatggcgg cttctattag tatcgttccg gttccgcaaa aaggatcgat tagtattct
//
LOCUS       DAOPWC010000272         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52409_length_2218_cov_3.291262, whole genome shotgun sequence.
ACCESSION   DAOPWC010000272 DAOPWC010000000
VERSION     DAOPWC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52409_length_2218_cov_3.291262"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PKJ65_06025"
     CDS             complement(<1..1384)
                     /locus_tag="PKJ65_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HNZ41426.1"
                     /translation="MFITEAVVLKEDKVLKAADLSEEQMQRARKGTIAHSILKSHNTS
                     ASNHMLKIKFDAVASHDITYVSILQTASACNMERFPIPYVLTNCHNSLCAVGGTINED
                     DHLFGLSAAKKYGGIYVPAHLAVIHQYMREKMAGCGKMILGSDSHTRYGALGTLAMGE
                     GGGELVKQLLGKTYDLPYPKVCLVYLTGTLNQGVGPHDVAIYMIGKVFKDASVTNKIL
                     EFAGPGVKNLSMDDRIGIDVMTTETACLSSIWVTDDKTKEYYEIHGRKEDYKKLSPAK
                     EALYDCALMIDLSKIQPMIALPYHPSNAYTVKDFLENSEDILEQLNLGSLKVYADSKK
                     RSFFTGQGIIAGCAGGSFENICEAAAVLQKGDTGYGSFSLSVYPQSQPVAYALNKAGI
                     TNKLMESGVVIETAFCGPCFGAGDIPANNVLSVRHTTRNFENREGSIPKDGQAAYVAL
                     MDARSIAATAL"
     gene            1464..2087
                     /locus_tag="PKJ65_06030"
     CDS             1464..2087
                     /locus_tag="PKJ65_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ41427.1"
                     /translation="MKYKHIIWDFDGTLFDTYGEIAKVIEEVLKNRGIVENLDWIAKN
                     LHMSLTHTLEYLSKKHNLNHGEFKGTFMQAEERMNVKEALPFPGVKEIIDSTEGYTFM
                     VTNRAESVFRFLEYRDYRKYFKEILHRNSGFPLKPGSEAVEYLIEKYGLDRKEMLFIG
                     DRDVDIECAINAQIHCCYYDSHNIPNIASCTYYTKDYFKLLDILKTA"
BASE COUNT          658 a          489 c          431 g          640 t
ORIGIN      
        1 tcaatgcggt agcggcaatg cttctggcat ccatcagtgc gacatacgcg gcctgaccgt
       61 ccttggggat gctgccttca cgattctcga aattccttgt ggtatgcctt accgacagga
      121 cattgttggc aggtatgtca ccggcaccga aacacggtcc gcagaatgcc gtttctataa
      181 caacacccga ttccataagc ttgtttgtaa tccccgcctt attaagcgca tatgcaaccg
      241 gctgactttg aggataaacg gacaaggaga aactgccata tccggtatca cctttttgca
      301 aaacagccgc agcttcacag atattctcaa aagaacctcc cgcacatccc gctattattc
      361 cctgtcccgt aaaaaagctt ctcttcttac tgtcggcata tactttcaga ctgccgagat
      421 tcaactgttc cagtatgtct tcactatttt ccaaaaagtc cttaacggta tatgcattgg
      481 aaggatgata cggcaaagct atcatgggtt gtattttgga aaggtcaatc attaaggcac
      541 agtcatacag cgcttctttt gccggagaaa gctttttata atcttccttc ctcccgtgta
      601 tctcatagta ctcttttgtt ttatcgtcgg ttacccatat ggaagaaaga catgcagttt
      661 cggtggtcat aacatcaata cctattctgt catccatgga aagattttta actcccggtc
      721 ccgcaaactc caatattttg ttggtaaccg aagcatcctt aaatactttt cctatcatgt
      781 atatggctac atcgtgaggt cccactccct gattaagagt acctgtaaga tatacaagac
      841 ataccttggg atacggaaga tcataggttt ttcccaacag ctgctttaca agttctcctc
      901 cgccctcacc catggccaag gtgcccaacg ccccgtacct tgtgtggctg tccgacccta
      961 aaatcatctt gccgcacccc gccattttct cccgcatgta ctgatgtatt accgcaagat
     1021 gagcgggtac ataaatgcct ccgtattttt ttgccgcgga caaaccgaaa agatggtcat
     1081 cttcattgat ggtaccgcct acagcacaaa gagagttatg gcaattggtc agaacataag
     1141 gaataggaaa gcgctccata ttacatgccg aagcggtctg taaaatactt acatatgtaa
     1201 tatcatggct cgcaacagca tcaaatttaa ttttgagcat atgattcgaa gcggatgtat
     1261 tatgtgattt caatatagaa tgagcaatgg tacccttgcg tgctctttgc atctgttctt
     1321 cagacagatc cgctgctttc agtactttat cttccttcaa aactactgct tcggttataa
     1381 acatcggaaa atcatccttt ctatgttata ctttattata cttgaacgga aaggttatgc
     1441 aaatgcataa atatgattga ataatgaaat acaagcatat tatctgggat tttgacggaa
     1501 cgctgtttga cacatacgga gagattgcga aagttatcga agaggttttg aaaaacagag
     1561 gaatagttga gaatcttgat tggatagcaa aaaacttaca tatgtcactg actcatacac
     1621 tggagtatct ctcaaaaaag cacaatctga atcatggcga gtttaaaggt acattcatgc
     1681 aagccgaaga aagaatgaat gttaaagaag cattaccgtt ccccggagta aaagaaataa
     1741 tagacagcac cgaaggatac acttttatgg ttacaaatcg tgccgagtcg gtatttcggt
     1801 ttctggaata tcgggattac aggaaatact ttaaggagat cctgcacaga aattcgggat
     1861 ttcccttaaa acccggcagc gaagcagtcg agtatctgat agaaaaatac ggtcttgaca
     1921 gaaaagaaat gctgtttata ggagacaggg atgtggatat cgaatgcgca ataaatgcac
     1981 aaatccattg ctgttattac gattcgcata acattccgaa tatcgcatcg tgtacatact
     2041 acacaaaaga ttactttaag ctacttgaca ttttgaaaac cgcttaaagc attagtcaca
     2101 attattatag caacaggaat aatgtatatc gtaaaagcaa ccagaaacgc cacttttccg
     2161 ttactgtcca gtttcgtctc tttaataata ttaatctttg tactttcggt attttttc
//
LOCUS       DAOPWC010000273         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_52515_length_2215_cov_3.741667, whole genome shotgun sequence.
ACCESSION   DAOPWC010000273 DAOPWC010000000
VERSION     DAOPWC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52515_length_2215_cov_3.741667"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKJ65_06035"
     CDS             complement(<1..1005)
                     /locus_tag="PKJ65_06035"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ41428.1"
                     /translation="MRKRTFLTISVLLVAVILSSCTPVETPTVQPSSVPSETPPQTSF
                     SPQPPASEQPTETRPAEAAPKTATLKLSATGDVMSHYTQILDAYNAETGEYDYSNSLS
                     RIKNFTKTSDLLIANLETSMAEGEYTGYPIFNTPPALAKNLKDIGVDIVSTANNHCLD
                     KGFKGLVSTIENVKAAGLLQVGTYAAQQDRDASHGVLVKEINGISMAIVNYSYGTNVI
                     RIPAGKEFCVNLLYEDYLGPYTILNKKQISSDLEYARSLDTDLIIALIHWGTEYVTTE
                     SEKQRQAAQFLFDNGVDILIGNHPHVLQPMGIQTIRTPEGQEKQVFVCYSLGNFMSNI
                     T"
     gene            complement(1038..>2215)
                     /locus_tag="PKJ65_06040"
     CDS             complement(1038..>2215)
                     /locus_tag="PKJ65_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNZ41429.1"
                     /translation="KQVSAGSLHTAAVTTAGSVLTMGSNSAGQCNIGIIAGVSKVECG
                     SMHTVCLRSNGTVRSVGANFFGETNTSGWSDIVSVAAGNGFTLGLKSDGTVLATGNNR
                     FKQTDVSSWHNIIEVFAGYNYSVGLKADGTLVYTGLATEMSSSLQELSNIKTLSCNRD
                     MMILKTNDNEIIILGNTGAQERSTNKVYVYSFDDIAQDYWAATYINLVSRLGIINGVY
                     SGTGYIYIPHVHMTRQEFFKVILETMSIPTNVDADIEIFEDYEDVDSWSLPYIKTAYY
                     YGYLTGSTDDEGKVYIRPKDPISRAEAAVIMGRVIGLTESPDMEYADKDTVPLWAYDA
                     ISYFSHMKVLNGYPDGTIAPNNNLYRSEAAAIICRLYSYVLNAYTFNHVDNVAETAD"
BASE COUNT          582 a          497 c          472 g          664 t
ORIGIN      
        1 ggttatattg gacatgaagt tacctaacga ataacatacg aatacctgct tttcttgacc
       61 ttccggggtg cgtatcgtct gtatccccat aggttgcagt acatgggggt ggtttcctat
      121 caatatatcc acgccgttat caaacagaaa ttgtgcggct tgtcgttgct tttcgctttc
      181 cgttgtgaca tattccgttc cccagtgaat aagggctatt ataaggtcgg tatcaagaga
      241 gcgtgcatac tcaaggtcgg aagatatctg ttttttgttt aatattgtat agggacctaa
      301 atagtcttca tacagaagat ttacgcagaa ctcctttccc gccggtatcc ttataacatt
      361 ggtaccgtag gaataattaa ctatcgccat acttattccg ttaatctctt taaccagaac
      421 tccatgtgac gcatcacgat cttgctgtgc ggcatatgtt cccacctgaa gaagtcccgc
      481 cgcttttaca ttctctatgg tggagacaag gcctttgaaa cctttatcaa gacagtggtt
      541 gttagcggtg cttacaatat ccacccctat gtccttaagg tttttggcca aagcgggagg
      601 agtattgaaa ataggataac cggtatattc tccctccgcc atggaggttt ccaaattggc
      661 aataagaaga tcgctggttt tagtaaaatt tttaattcga cttaaagaat tggaataatc
      721 atattcgccg gtttcagcat tatatgcatc taaaatctgg gtgtagtgac tcattacatc
      781 gcctgttgcc gacagtttca aagtagctgt tttcggggcg gcttccgcag gtcgggtttc
      841 cgtcggttgc tccgaagcgg gcggttgggg agaaaaagaa gtctgaggcg gagtttcgga
      901 aggtacggag gacggttgta ctgtaggtgt ttccaccgga gtacaggagg acagtataac
      961 cgcaacaagc agtactgata tagttaaaaa cgttcttttc ctcactttac tacctcttct
     1021 tctttcatat tgtgtgctta atctgcagtt tctgctacat tatcgacatg attaaaagta
     1081 taagcattca acacataact gtaaagtctg cagatgatag cggcagcttc cgaacggtat
     1141 aagttgttat tcggtgcaat tgtgccgtcc ggataaccgt tgagcacctt catatgtgaa
     1201 aagtaagaga tcgcatcata tgcccacaag ggtacagtat ctttatcggc atattccatg
     1261 tcgggacttt ccgttaaacc tattacacgc cccattatga ctgcagcttc cgcccgagaa
     1321 atcgggtctt taggtcttat atacacctta ccttcatcat ctgtacttcc ggtaagatat
     1381 ccgtagtaat atgcggtttt aatgtacgga agactccagg aatccacatc ttcataatct
     1441 tcgaaaatct caatatccgc atcaacattc gtaggaatgc tcatagtttc cagaataact
     1501 ttgaagaatt cctgtctggt catatgcaca tgagggatat atatgtaccc cgttccgctg
     1561 tatacaccgt taataatacc caatcgggat acaaggttta tgtatgtagc agcccagtaa
     1621 tcctgtgcta tatcgtcaaa gctgtataca taaactttat ttgtcgaacg ctcctgtgcg
     1681 ccggtgtttc ccagaataat aatttcatta tcatttgttt tgagaatcat catatctctg
     1741 ttgcaggaga gagtcttgat attgcttaat tcctgcagag aagaggacat ctccgttgct
     1801 aatcctgtat atacaagcgt tccgtccgcc ttaagtccca cagagtaatt ataccctgca
     1861 aagacctcta tgatattatg ccatgatgag acatccgtct gtttaaagcg gttgttgccg
     1921 gttgccaaca cggtgccgtc ggatttcagt cccagcgtga aaccgtttcc cgcagcaacg
     1981 gaaactatat ccgaccaacc cgaagtgttg gtttcaccga aaaagtttgc gcctacactt
     2041 ctgacagtac cgttggatct taaacataca gtgtgcattg aaccgcattc cactttggaa
     2101 actcccgcta taataccgat attgcattgg ccggcactgt tgcttcccat agtcaaaacg
     2161 gaacctgcgg ttgtaaccgc cgcggtatgg agagaacctg ccgaaacctg tttta
//
LOCUS       DAOPWC010000274         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_53315_length_2194_cov_2.785414, whole genome shotgun sequence.
ACCESSION   DAOPWC010000274 DAOPWC010000000
VERSION     DAOPWC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53315_length_2194_cov_2.785414"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PKJ65_06045"
     CDS             complement(<1..1772)
                     /locus_tag="PKJ65_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ41430.1"
                     /translation="MKTDVTKRRNIIKNIAIVFLAVMLVLTFFSNTILNYSLPEVAVQ
                     YPMYGTISSRIRGQGTVTANQTYQIYFDESRVIKSVDVRRGEIVQKGAVIYRLEDAPS
                     DELNNANNTLTTMYINYKRALMQSTYDYSGILEEISELNKLLKEAVSIKANLEINKLL
                     YADLQNKIRLAENKREELSVKKQELEQYLNSLKPDTSYSDEEFPVAYAIAKTDYENSK
                     AMLDAEKENLKNLQRQLVAMANPVLEAQSAVDAKIQERDDYIALHPDTDVPTMTEIEA
                     KENELEAAENEVPPDADKIKRIKNELNAMIYRYFEYLPVREQIDEYNKQIENLTKALN
                     TVKNQERDINTAITFSRNRIDVLQFTHDDLYEIYLPLKARYEYGDKEAELVNVTAELI
                     SAQEEVNALNERKSTVEAKVMTEAEADALVVQRQKELNDKQASYEKLKAQNELDEQIN
                     KMNLENMKREIIEQEALVKRLQEKAINGVITAPVTGMIINLTYVTGEKTNSGAVVAEI
                     EISEKGYTMSFTVTIEQSRRLSVGQVAQVQYYYGSNAPYAEISAIKTDYNNPRGSRIV
                     ELEVRGDNIYPGQTMYVALGETGR"
     gene            complement(1785..>2194)
                     /locus_tag="PKJ65_06050"
     CDS             complement(1785..>2194)
                     /locus_tag="PKJ65_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ41431.1"
                     /translation="WSIILPGIFSPFAVFILTKTMQRIPSSIVEAAKIDGANEWQIFG
                     KVFVPMSRNAIYSVALLLFIDYWNMVEQPLIMLQNNEDMHPLSVFLSKINAGEIGLAF
                     AVAAIYMVLPLLLFLYSEDRILEGIASSSAIKG"
BASE COUNT          539 a          524 c          366 g          765 t
ORIGIN      
        1 tcccttccgg tttctcccaa cgcaacatac atggtctgcc cgggatatat gttatcccct
       61 cttacttcca gttccactat tctgcttccg cggggattgt tataatcggt tttaattgcc
      121 gatatttccg catacggcgc attggagccg taatagtact gaacttgtgc aacctgtccg
      181 acactcaatc ttctgctttg ctctatagtt accgtaaaac tcattgtgta acctttttca
      241 ctgatctcta tttccgcaac caccgctccc gaattggtct tctctccggt gacatatgta
      301 agattaataa tcattccggt taccggagcg gtaataactc cgttaattgc cttttcctgt
      361 aatcttttaa ccagcgcttc ctgctcgatg atttccctct tcatattttc caggttcatc
      421 ttatttatct gctcatccaa ctcgttctga gccttcagtt tttcatatga agcctgcttg
      481 tcattcagtt ctttctgtct ttgcacaacc aatgcatcgg cctccgcctc tgtcattacc
      541 ttagcttcca cagtactttt gcgttcatta agtgcgttga cctcttcctg tgccgaaatg
      601 agttcggcgg tcacatttac caattccgct tctttatcac cgtactcata tctcgccttt
      661 agaggaagat atatctcata caagtcgtca tgtgtaaact gaagtacatc tattctgttc
      721 ctcgaaaaag taatagccgt atttatatct ctctcctgat tctttacggt atttaatgct
      781 tttgtaaggt tttcaatctg cttattatac tcatcaatct gctcacgaac cggcaggtat
      841 tcaaaatatc ggtaaatcat cgcattgagt tcattcttaa ttctttttat tttatccgca
      901 tcgggcggca cttcattctc ggcagcttcc agttcattct ctttagcttc aatctccgtc
      961 attgtcggta catcggtatc gggatgtaag gctatataat cgtctctttc ctgtatcttg
     1021 gcatccacag ccgattgtgc ttcaagtacg ggatttgcca ttgctaccag ttgtctttga
     1081 agatttttca gattttcttt ttccgcatcc aacatggctt ttgagttttc atagtcagtt
     1141 ttagctattg cataagcaac gggaaattct tcatcgctgt atgaagtatc gggttttaac
     1201 gagttgagat actgttccag ctcctgtttc tttaccgata attcctccct tttattctcg
     1261 gctaacctta tcttattctg taaatccgca tataaaagct tatttatttc aagatttgcc
     1321 tttatactca cggcttcttt aagcaatttg ttaagttccg atatttcttc aagaataccg
     1381 ctgtaatcgt atgttgattg catcagtgct ctcttatagt tgatatacat agtggtcaga
     1441 gtattattgg cattgttaag ttcatcgctc ggagcgtctt ccagacgata tataacggca
     1501 cctttttgta caatctctcc tcttctgaca tccacactct ttattacccg ggattcatca
     1561 aaatatatct gatatgtctg attggcggtt accgttccct gacctcttat tctggaagat
     1621 attgtaccgt acataggata ctgtacggct acttccggta aagaataatt cagtatggtg
     1681 ttggagaaaa atgtcagcac cagcattacc gctaaaaaca caatcgctat attctttatg
     1741 atgttccttc ttttagttac atccgtcttc ataaaaaacc tccattaacc ttttatagca
     1801 ctggaacttg ctataccttc cagtatcctg tcctcactgt acaggaaaag cagcaacggc
     1861 aataccatat atatagccgc aactgcaaaa gcaagcccta tttctccggc attaattttg
     1921 gaaagaaata ccgaaagagg atgcatatcc tcgttattct gtaacataat caacggttgc
     1981 tctaccatat tccagtaatc tatgaataac agcaacgcta cggaatatat tgcattcctt
     2041 gacataggta caaatacttt accgaatatc tgccactcat tggcgccgtc tatttttgcc
     2101 gcttccacta ttgatgaggg tattctctgc attgttttgg taagtataaa caccgcaaac
     2161 ggagaaaata ttcccggcag aataatggac caac
//
LOCUS       DAOPWC010000275         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_53633_length_2186_cov_3.415767, whole genome shotgun sequence.
ACCESSION   DAOPWC010000275 DAOPWC010000000
VERSION     DAOPWC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53633_length_2186_cov_3.415767"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKJ65_06055"
     CDS             complement(<1..1277)
                     /locus_tag="PKJ65_06055"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HNZ41432.1"
                     /translation="MKTNAITYLLTKKLKNSIKELFKKPARLIYAIVMIALIVVTAIS
                     GKKNVDENTVFRDLRELTAGITALYILIFGLIAYNGFKNGATMFKMPDVNLLFTSPMH
                     PRKVLFYGLFQQLGTSLLLGVFILYQYSWLHILYGINFLHLIIILLGYSLSVFVAQLT
                     AMVIYSFTSYDDKKKRIAAVIFFGIIIAYAIGLVMFLLKDMDNVLTNLVTAGNSIFLR
                     LMPGGGWIGGILNGILLKDVSVMLLGIALTAAYIAGMTILLSYAKQDYYEDVLKTSEE
                     QFSLASAQKEGKIMETVGKIRVGKTGLSKGEGASAFYYKHKLEERRARRFILEPAGIV
                     FMIVSIVFAVFMRKYGIASIFAYTTYMQMISVKMGRFSKELGKPYIYLVPESSFKKIL
                     YSIAQSFAGFLVEASILYIALAFILKLTPYDVV"
     gene            complement(1274..1984)
                     /locus_tag="PKJ65_06060"
     CDS             complement(1274..1984)
                     /locus_tag="PKJ65_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ41433.1"
                     /translation="MLEVLNVTKKYGKVVANDDISFSVNDGQIAILLGPNGAGKSTII
                     KCISGLLRFKGEIKLDGHDNKSIEAKRLLGYVPEVPAVYDLLTVEEHLEFIARAFRVK
                     DDEYERSLLERFELWDKKAKLGKELSKGMQQKLSICCALVHKPEVIVFDEPLVGLDPH
                     AIKVIKAIFKELKQDGRTILISTHMIDSVEDYWDIAHIMMDGKFAATKLNESVSGDDK
                     ESLEELFFRITEKKSEEA"
BASE COUNT          643 a          476 c          360 g          707 t
ORIGIN      
        1 atgacaacat cgtacggcgt aagttttaat ataaaagcaa gtgctatata taatatagac
       61 gcttctacta aaaagccggc aaaagactgg gcaatgctgt acaatatctt tttgaaagaa
      121 gattccggta ccaggtatat ataaggtttg ccgagttctt tggagaaacg acccatcttg
      181 accgatatca tctgcatata tgtagtatat gcaaaaatgg aagctattcc gtattttctc
      241 ataaatacgg caaatacgat ggaaacaatc atgaatacta taccggccgg ttccagtatg
      301 aatcttcttg cccttctttc ttccagtttg tgcttgtagt aaaaagcact tgcaccttct
      361 cctttggaaa gaccggtttt gcctacccta atcttgccga ccgtttccat tatcttgcct
      421 tctttctgtg ccgatgcaag tgaaaactgc tcttcgcttg tctttaagac atcttcgtag
      481 tagtcctgtt ttgcataaga aagcaaaatt gtcatccccg ctatatatgc tgcggttaac
      541 gctataccga gaagcatgac ggaaacatcc ttaagcaata taccgttaag tattccgcct
      601 atccatcctc ccccgggcat aagtcgcaag aatatgctgt ttcccgcagt tacaagattg
      661 gttagtacat tatccatgtc tttaagtaag aacattacca gtcctattgc atatgcaatg
      721 attataccga agaaaataac agcggctatt cttttctttt tatcatcata actggtgaag
      781 gaatatataa ccatagccgt caactgtgca acaaacaccg ataatgaata acctaacagt
      841 ataatgataa gatgcaaaaa gtttattcca tacagaatgt gcagccacga gtattgatat
      901 aagatgaaaa ctcccagaag taaagaggtg cccaactgtt ggaataatcc gtaaaacagc
      961 acttttctgg gatgcatggg tgatgtgaat aagagattca catccggcat cttgaacatt
     1021 gtcgctccgt ttttaaaccc gttataggct ataagaccga atattaatat gtacaatgcc
     1081 gtaatacccg ctgtcagttc tctaagatca cgaaatacgg tattttcatc cacattcttt
     1141 ttccccgata tggccgtaac gactattaat gctatcatta caatggcata tatcaagcga
     1201 gccggtttct taaacaattc cttaatactg tttttcagct tctttgtcag aaggtatgta
     1261 attgcgtttg ttttcatgct tcttcactct ttttttcggt aatcctgaaa aacagttctt
     1321 ccagactttc cttgtcgtca ccggaaacgc tttcatttaa ttttgtagct gcaaattttc
     1381 cgtccatcat tatatgagcg atatcccaat aatcttcaac gctgtcaatc atatgggtac
     1441 tgatcagtat tgttcttccg tcttgcttca attccttaaa tatggctttt ataaccttaa
     1501 tggcatgagg atccagacct accaaaggct catcaaatac aatgacctcg ggtttgtgta
     1561 ccaatgcaca gcatatggat aatttctgct gcattccttt agacaattct ttgcccaatt
     1621 ttgctttttt gtcccaaagt tcaaaccgct ccaaaagact tctctcatat tcgtcatctt
     1681 ttactctgaa tgctctggct ataaattcca gatgctcttc cactgtcaga agatcatata
     1741 cggcgggaac ctccggtaca taccccaata accgctttgc ttcaatggat ttattatcgt
     1801 gtccgtccag ttttatctca cccttgaaac gcaacagacc gcttatgcat ttaataattg
     1861 tggatttacc ggcaccgttg ggacctaaaa gaattgctat ctgtccgtca ttaacagaga
     1921 aagatatatc atcgtttgcc accacttttc cgtacttttt cgttacattc aatacttcca
     1981 gcattttttc tcacctttat atattaaaaa tcaaattttc cctacctatc agatatttta
     2041 tcactactaa tacatataaa aatcaatttt ctgcaaatta ttgtactgct tgagttaaat
     2101 gtatatgtat aagttcaaaa gaaaggtaaa ttgcagaaat agcagcgaat ttaaaggaga
     2161 gccttccggc tctcttttaa cattag
//
LOCUS       DAOPWC010000276         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54149_length_2171_cov_2.900284, whole genome shotgun sequence.
ACCESSION   DAOPWC010000276 DAOPWC010000000
VERSION     DAOPWC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54149_length_2171_cov_2.900284"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..807
                     /locus_tag="PKJ65_06065"
     CDS             91..807
                     /locus_tag="PKJ65_06065"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HNZ41434.1"
                     /translation="MKILTIGNSFSQDATAYMERIALSDIYVRNLFIPGCDLKMHCDN
                     IKTNEKAYEYQKNGKKLCMISLEKALNKEQWDVITIQQVSHLSGILNSYFPYISELIK
                     YIRQNVPDAKIMLHKTWPYAHGCLHSGFSDYNNNTKTMYDSINRTVKIISAQFNIPVI
                     NTGDCIYNAYTECLFNPDYEGISLFRDGFHLSIPHGRYLAALCFYLHFYNKDRINLKY
                     LPKGMTHKQRDCLQSLAMKH"
     gene            881..1186
                     /locus_tag="PKJ65_06070"
     CDS             881..1186
                     /locus_tag="PKJ65_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNZ41435.1"
                     /translation="MVRTSNIYVRVEPYIKEQAEEILEKLGIPMSNAVSIFLRQVVMH
                     NGLPFDIKIPDSKPLSLPILSEAEFNMEMLKAHNDFENGRIYSLEEVEDELKKELGK"
     gene            1183..1518
                     /locus_tag="PKJ65_06075"
     CDS             1183..1518
                     /locus_tag="PKJ65_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ41436.1"
                     /translation="MKFKIIFSENAKKDLISIVRYISEELLEPVTAEKLSQRILKAIK
                     SLNEFPMRHRLCDCKEWKDKGLRVLPVENYLVFYIPDEIAHNVKIYRVIYGKRDIENQ
                     LKDRIAFDE"
     gene            complement(1529..>2171)
                     /locus_tag="PKJ65_06080"
     CDS             complement(1529..>2171)
                     /locus_tag="PKJ65_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNZ41437.1"
                     /translation="DTLRFCLSEFIWELSPYYLQIENMLMKGKRIDAIGFQYHLFKTP
                     ERLEREAEILLNPTCLFNVMDTYGKFHKPMHVSEVTVPAFGLTDENKEVQAKLTEWLY
                     KVWFSHPYMESIVWWNLVDGTAAYAPLGSAEGENYYGGGLLNYDMSKKPVYHAINNLI
                     NKEWHTDLKFNKNECLFNGFFGEYDIEVIADDKKTDHKIHLSKDGSREFVLTV"
BASE COUNT          740 a          368 c          399 g          664 t
ORIGIN      
        1 gggtgttctg gttttatgta gtaaatacgc ttatggtttc tttggatatc attatatatt
       61 ttaataacag gaagagagat aaaaagaagg atgaagatac tcacaatagg taacagtttc
      121 tcgcaggatg ctaccgcata tatggaaaga atagcgctgt cggatattta tgtaaggaat
      181 ctctttatac cgggatgtga cttgaaaatg cattgcgata atattaaaac aaacgaaaaa
      241 gcatatgagt atcagaaaaa cggtaaaaaa ttatgcatga tatccttgga aaaagcccta
      301 aataaagagc aatgggatgt tattacgata cagcaggtca gtcatcttag cggtatattg
      361 aacagctatt ttccgtacat ttccgaactt ataaaatata taagacagaa tgtcccggat
      421 gcaaaaataa tgttacataa aacatggccg tatgctcacg ggtgccttca ttcgggattt
      481 tccgattaca acaataatac aaaaacaatg tatgacagca taaacaggac ggtaaagatt
      541 atttccgctc agtttaatat accggtaata aataccggag attgtatata taatgcatat
      601 acggaatgcc tgttcaatcc cgactatgaa ggtatatcac tcttccgtga cgggtttcat
      661 ctgtccatac cgcatggaag gtatcttgcg gcactgtgct tctatttgca cttctacaat
      721 aaagaccgaa taaacttaaa atacctgcct aaaggtatga cacataagca aagggattgc
      781 ctgcagtctc ttgccatgaa gcactgacgg aaagtttgcg aatgtattgc taatcacaat
      841 tcaatttgct ataatgtaag cgaaaggggg agcaatatat atggtaagaa catcaaatat
      901 atatgtaaga gtagagcctt atatcaaaga acaggcagaa gaaatactgg aaaaactcgg
      961 aataccaatg tcaaatgccg tatcaatttt tttgcgacag gttgtcatgc ataacggttt
     1021 accttttgac atcaaaatac ctgattctaa accgctttct ttgcctattt tatcagaagc
     1081 ggaatttaat atggaaatgt taaaggctca taatgacttt gaaaacggaa gaatatacag
     1141 tttggaagaa gtggaagatg agttaaagaa agagttagga aaatgaaatt taagataata
     1201 ttttctgaaa atgctaaaaa agatttaata agcattgtaa gatatatttc agaggagttg
     1261 ttggaacccg taactgctga aaaattatca cagagaatat taaaagctat aaaatctctt
     1321 aatgaatttc cgatgcggca tagattatgt gattgcaaag agtggaaaga taaaggcctt
     1381 agagttttac ctgtagaaaa ttatcttgtt ttttatatcc cggatgaaat agcgcataat
     1441 gtaaaaatat atcgagtaat ctatggtaaa agggatatag aaaatcaatt aaaagataga
     1501 attgcatttg acgaatagta taaaatcatc agacagtcaa tacaaattct ctgcttccgt
     1561 ctttacttaa atgtatttta tggtcggttt ttttgtcatc tgcaataact tctatatcat
     1621 attctccgaa aaacccgtta aataagcatt cattcttatt aaatttcaaa tccgtatgcc
     1681 actctttgtt tatcaggtta tttatagcat gataaaccgg tttcttactc atatcataat
     1741 tcaataaccc gccgccgtaa tagttttctc cttccgcgct tcccaaaggt gcgtatgctg
     1801 cagttccgtc cactaagttc caccaaacaa tggattccat ataggggtgg ctgaaccaga
     1861 ctttgtacaa ccattcagtt aattttgctt gaacctcttt attctcatct gtcagaccga
     1921 atgccggaac ggttacttcc gaaacatgca tcggtttatg gaattttccg tatgtatcca
     1981 taacattgaa taagcaggta ggattaagta atatctccgc ttctctttcc aatctttcag
     2041 gtgttttgaa cagatggtat tggaatccta ttgcatcaat tcttttacct ttcatgagca
     2101 tgttttcaat ctgcaggtaa taagggctca actcccatat aaattccgac aagcaaaatc
     2161 ttaacgtgtc g
//
LOCUS       DAOPWC010000277         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54270_length_2168_cov_3.163275, whole genome shotgun sequence.
ACCESSION   DAOPWC010000277 DAOPWC010000000
VERSION     DAOPWC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54270_length_2168_cov_3.163275"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKJ65_06085"
     CDS             complement(<1..309)
                     /locus_tag="PKJ65_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41438.1"
                     /translation="MIEVKRLHKSFGPKGSLEVLKGIDQTIQKGEKVVIIGPSGSGKS
                     TFLRCLNMLETPTSGEIYYNKQLITDRLYDINKLRMNIGMVFQHFNLFPHLTILQNIT
                     L"
     gene            complement(296..1015)
                     /locus_tag="PKJ65_06090"
     CDS             complement(296..1015)
                     /locus_tag="PKJ65_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNZ41439.1"
                     /translation="MMKENFSEFIEQFKISFVNNFITDNRWQYLTKGLLVTLEITFFA
                     VMIGIILGFLMAVVRTTHDKTKKFKVLNAICKGYITVIRGTPVVLQLLIIYYVVFATV
                     AVDKVLAAIVAFGLNSAAYVAEIVRSGIMSVDSGQMEAGRSLGFTHGQTMWYIVLPQA
                     FKNILPALGNEFIVLLKETSVSGYIALADLTKGGEIIRSRTYDAFMPLIAVALIYLGI
                     VMLLTYLVNKLERRLAKSDRG"
     gene            complement(1075..1863)
                     /locus_tag="PKJ65_06095"
     CDS             complement(1075..1863)
                     /locus_tag="PKJ65_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNZ41440.1"
                     /translation="MKKIIATVLTTILALSALAGCTQVTPNTVFSADDLPGKVIGVQL
                     GTTGDIYASDYEAEGSVIERYNKGADAVQALKQGKVDCVIIDNEPAKAFVAKNKDLMI
                     LPDPFELEDYAMCIAKTNSELKNKINAAIAQLKIDGTLAAIISNYIGDDTKGKSPYVS
                     GNSDYSNGKLIMATNAHFEPYEYMEGGVVVGIDVDLAQAVADILKMELVIEDMDFDAI
                     IAAVQSGKADIGVAGMTVTEDRLISVDFTDSYTTATQVIIVRKK"
BASE COUNT          622 a          490 c          380 g          676 t
ORIGIN      
        1 caaagtaata ttttgcaata tggtaaggtg cgggaaaaga ttgaagtgct gaaacaccat
       61 accaatattc atacgcaact tgtttatgtc atacaatctg tcggttataa gttgtttatt
      121 gtagtatatt tctcccgaag tgggagtctc caacatatta agacaacgta agaatgtgct
      181 tttaccgctg cccgaaggcc cgataatgac tactttttcg cctttttgta tagtctgatc
      241 tattcccttt aacacttcca acgacccctt aggtccgaag cttttatgta gccgtttaac
      301 ctctatcact tttagcgagc ctcctttcca gcttatttac cagataagtg agcagcatga
      361 ctatacccag atatatcaat gccaccgcta taaggggcat aaatgcgtca tatgtcctgc
      421 tgcggataat ctctccgcct tttgtcagat cggccaatgc gatatagccg gaaaccgaag
      481 tctccttgag taacactata aactcattac ccaatgcggg aagaatgttc ttgaatgcct
      541 gcggcaatac tatataccac atggtctgac cgtgcgtaaa gcccaaggat cttccggctt
      601 ccatctgccc ggaatctaca ctcattattc ccgaacgcac tatttccgca acatatgcgg
      661 cggaattcag tccgaaagcc acaatcgcag ccaatacctt atccaccgca acggtagcaa
      721 aaacaacgta atagattatc agaagttgca ataccaccgg cgttcctctt attaccgtta
      781 tatatccttt acagattgca ttgagaactt taaacttttt tgtcttgtca tgagttgtgc
      841 gtacgaccgc cattaaaaat cccagtataa tacctatcat aacggcaaag aaagttatct
      901 ccaaagttac cagtaatcct ttggtaaggt actgccatct gttatcggtt atgaaattat
      961 tgacaaagct tattttaaac tgctctataa actcgctgaa attctctttc atcattacct
     1021 ttccgacaaa catcggagtc ggaagatatc cgactctgac gcctgtattt aaaactactt
     1081 cttccttact attattacct gtgtagccgt agtgtatgaa tcggtgaaat ccacacttat
     1141 aaggcgatct tcggtaacag tcattccggc cactcctatg tcggcttttc ccgactgaac
     1201 ggcggcaata attgcgtcaa aatccatatc ttctataacc aattccattt tgagtatgtc
     1261 ggctaccgct tgtgcaaggt ccacatctat tcccactaca actccgcctt ccatatattc
     1321 atacggttcg aaatgtgcgt ttgtagccat tatgagtttg ccgttgctgt aatcggaatt
     1381 accggaaaca tacggactct taccttttgt gtcatctcct atgtagttgg agataatggc
     1441 agccagcgtt ccgtctatct ttaactgagc tattgcagcg tttattttat tcttaagttc
     1501 ggaattggtt tttgctatgc acatggcata atcttccagc tcaaacggat cgggcaatat
     1561 cataaggtct ttattctttg ccacaaatgc ttttgccggc tcgttgtcga taatgacaca
     1621 gtccacttta ccttgtttca aagcctgaac ggcatcggca cctttattgt atctctcaat
     1681 aacggaacct tccgcttcat aatcggaagc atatatgtct cctgtggtgc ccagctgaac
     1741 ccctataact ttgccgggca gatcgtccgc ggaaaacacc gtattgggtg ttacctgtgt
     1801 gcagcccgcc aaggcagaca gtgccagtat tgttgttaaa acagttgcta taatcttttt
     1861 cataaaatat attaccctcc gtaagcaatt tcgtgttttc ataaatatac acttatgtgc
     1921 ataaatagtc aatagcaaag tttgaatttc gttataattt tccgttttta attaaatatc
     1981 gataaaacca atgaatatta ttcattttca ttgcataaat tgatgtataa atcattaaaa
     2041 gaataagaac atataaagat atggtagatt gcataactaa gttccgttct tacataaccc
     2101 aaaaaagcga attaccgata aagtgttaaa aaagacataa aatcgttgaa atatttcaat
     2161 ttgaatga
//
LOCUS       DAOPWC010000278         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54284_length_2168_cov_2.625651, whole genome shotgun sequence.
ACCESSION   DAOPWC010000278 DAOPWC010000000
VERSION     DAOPWC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54284_length_2168_cov_2.625651"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKJ65_06100"
     CDS             <1..511
                     /locus_tag="PKJ65_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41441.1"
                     /translation="EHEKYENKINKYIDNLTDTDNILFDNRRLAYYSGCLLLIALKQA
                     DICFFHDISKETKTVYELISEGFPAADLPEILHKYAIEESLKRYIDNRKKLFSDFFSK
                     NPECTEKPYKICGYDPMNMVKLDNLVWCKYFVMLKEKAKEDPLLIYNQVVLKVDETDT
                     TSVSAYYTV"
     gene            complement(515..1549)
                     /locus_tag="PKJ65_06105"
     CDS             complement(515..1549)
                     /locus_tag="PKJ65_06105"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ41442.1"
                     /translation="MKEVYAVTGALGFLGNAIVDNLLSKGKTVRVMDIGQDRYGVLKD
                     KDVRFFRADVCDAASMEDFFTVEDDQELIVIHAAGIVSISSKFSQLMYDVNVKGTQNI
                     VDMALKHKIKKLVYVSTVHAIPELPKDEMIAEISDFNPNLVEGEYSKTKAIATKIVMD
                     SVQKGLYAMVVHPSGIIGPGDYAGGHMTQLVKDCAEGRLTACVRGGYDFVDVRDVAEG
                     IVLAAEKGKSGNAYILSGEYYPIKKITDTICSVLGKKQIKTILPLWFAKMTAPLAELY
                     YRIKKQKPLFTSYSLYTLQSNSNFNSKKAQQELGYKLKFSLKQSIEDTYMWIKANNLI
                     RRKKKVASKA"
     gene            1675..>2168
                     /locus_tag="PKJ65_06110"
     CDS             1675..>2168
                     /locus_tag="PKJ65_06110"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ41443.1"
                     /translation="MDNPFISHLRKPPKLKGIKRALFIQPHADDNQIGAGGMMALLAD
                     SGAEVYELTVTDDRRLEQDKEGLTTRQSEAVAAQQYLNVKNAGFLGFCDKTQEPVEKI
                     ALKIMEVIRKIKPDAVFTVDPDLAAECHSDHIKTGCAVKYAVMDAACDFYPYSEDGKA
                     RHET"
BASE COUNT          629 a          455 c          423 g          661 t
ORIGIN      
        1 ggagcatgaa aaatatgaga ataagataaa caaatatatt gataacctta ccgataccga
       61 caatattctt tttgacaaca gaagactggc atattattcc ggttgcttac tgttaattgc
      121 acttaaacaa gcggacatat gctttttcca tgatatttca aaagaaacca aaaccgtgta
      181 tgagctgata tcggaaggat ttcctgctgc cgatttacct gagattttgc ataagtatgc
      241 tattgaagaa tcgttgaagc gttatattga taacaggaag aagttattct ccgatttctt
      301 ttccaagaat cctgagtgta cggaaaaacc ctataaaatt tgcggatatg accccatgaa
      361 tatggttaaa ctggacaacc ttgtatggtg caaatatttc gtcatgctaa aagaaaaagc
      421 aaaggaagat cctttgctta tatacaatca ggttgttctt aaggttgacg agaccgatac
      481 aacctcggtg tcggcttact ataccgtctg atttttatgc ttttgatgca accttctttt
      541 tgcgtcttat gaggttatta gccttaatcc acatatatgt gtcttcaata gactgtttca
      601 aggaaaattt cagcttatat cccaactcct gttgtgcttt tttggagttg aaattggaat
      661 tggactgaag agtatacagc gaatagctgg taaataaagg cttctgtttc tttatcctgt
      721 aatacagttc cgcaagcgga gccgtcattt tggcaaacca aagaggaaga atggttttga
      781 tttgtttctt cccgagaacc gaacatatag tgtcggtaat tttctttatc ggataatatt
      841 cccccgataa gatataggcg ttaccgcttt tgcctttttc cgcagctaat actatacctt
      901 cggctacatc gcgaacatca acaaaatcat agcctcccct tacacaggct gtcagtcttc
      961 cctcggcaca gtcttttaca agctgcgtca tatgaccgcc ggcatagtct cccggcccta
     1021 taattcccga cggatgaact accatggcat acaagccttt ctgaacgctg tccatcacta
     1081 tctttgtggc tatggctttg gttttactgt attccccttc taccaaattg ggattgaaat
     1141 ccgatatttc cgctatcatc tcgtccttcg gtaattcagg aatggcatga acggtactta
     1201 catagaccaa tttcttgatt ttatgcttta atgccatatc cacgatattt tgagttcctt
     1261 ttacattaac atcatacatc aattggctga actttgatga tatcgagaca atacctgcgg
     1321 cgtggattac tatgagctcc tgatcgtctt caaccgtaaa gaaatcttcc attgaagcag
     1381 catcacatac atccgctctg aaaaaccgca cgtctttatc tttcaatact ccgtacctgt
     1441 cttgtcctat atccataact ctgaccgtct tccctttgga aagcaggttg tcaactatag
     1501 cgtttcccaa gaaccccaat gctcccgtaa ctgcataaac ttctttcatc cgttcatctc
     1561 ctttccgtac ttttaacata cattcagtac attctacatg tatgatataa ggctaagttg
     1621 aactgattat gaacaaataa tgggattatg atataatttt ttaagaggtg cgacatggat
     1681 aatcccttta tttctcattt aagaaaaccg cctaaactga aaggcattaa aagagctctt
     1741 ttcatacaac cccatgccga tgataaccag ataggtgcag gcggtatgat ggcattattg
     1801 gcagattcag gagccgaagt ttatgagctt acggtgacag atgacagaag attggagcag
     1861 gacaaagaag gtctcactac ccggcaatcg gaagcagttg ccgcccagca gtatttaaat
     1921 gttaaaaatg ccggattttt aggtttctgc gacaaaacgc aagaaccggt ggaaaagatt
     1981 gctttgaaga taatggaggt tataagaaag attaaaccgg atgcggtttt tacagtggac
     2041 cccgatcttg cggccgaatg tcacagtgat catataaaga cgggttgtgc ggtaaaatat
     2101 gcggtcatgg acgccgcgtg tgatttttat ccctattccg aagacggtaa ggcacgtcat
     2161 gaaacatg
//
LOCUS       DAOPWC010000279         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54423_length_2164_cov_3.922712, whole genome shotgun sequence.
ACCESSION   DAOPWC010000279 DAOPWC010000000
VERSION     DAOPWC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54423_length_2164_cov_3.922712"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKJ65_06115"
     CDS             complement(<1..371)
                     /locus_tag="PKJ65_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HNZ41444.1"
                     /translation="MTFLELLLIAVGLSMDAFAVAICKGLSLKKVTRKDALIVGLYFG
                     IFQAAMPLAGFLIGSVFAGIIDKWDHWVAFTLLAIIGIKMIIESKKKGSCQSSEDNSQ
                     SLSFKQMLPLAIGTSIDALAAG"
     gene            514..738
                     /locus_tag="PKJ65_06120"
     CDS             514..738
                     /locus_tag="PKJ65_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41445.1"
                     /translation="MDALFPLNKNVKKKKLSTLVIAILLYVVLSVIVGLIQWLLRGIP
                     VVNTVMNIVGYLVWLYSVVGVILAIFKFVK"
     gene            782..1309
                     /locus_tag="PKJ65_06125"
     CDS             782..1309
                     /locus_tag="PKJ65_06125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNZ41446.1"
                     /translation="MHDYTLIRSKRRTISLRISQDCTVIVRAPFLCSKKVIDKFVSDH
                     KKWIDRQIEIRKNQPSVKNLTSEQIKKLKKQAKQVLTERTKYYSEITGLYPAYIKITS
                     SEKRFGSCTQKGGICYSYRLILYPERAVDYVIVHELCHLRHRHHQKPFYDLVKKYMPD
                     YKERDKLLKNVTGML"
     gene            complement(1314..>2164)
                     /locus_tag="PKJ65_06130"
     CDS             complement(1314..>2164)
                     /locus_tag="PKJ65_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HNZ41447.1"
                     /translation="SEEAYDNYANYINKIKKMAGPDIEVYSMLAPSRSTYLALTKYDF
                     LSSRAANVEQINSKIDKSVKNVNVLRSMVEHVDEYIYYRTDHHWTHLGAYYGYTELMK
                     ARNRQSEIIPLSNYSEKLVVEGYLGFYNKPYLTQADYDNPDTIIAYYPIVDYKYIYNS
                     QDVSIEKRLINKERVKPDKDYYLLFSDGGFGTYSEITTSSDSDLKCMVIKDSFGDSLI
                     SFLLPHYKTIYVVDSRTYNKAYCGNMNIIDFAKSKNVDEIIVNYWITEITYMDDFMQK
                     AYATLE"
BASE COUNT          662 a          394 c          430 g          678 t
ORIGIN      
        1 ccggcagcaa gggcatcaat ggaagtgcct attgccaaag ggagcatttg cttaaaactt
       61 aaagattgac tgttgtcttc cgaagactgg caggagcctt tctttttgct ttctataatc
      121 attttaatac ctatgattgc caaaagagta aaagcaaccc aatggtccca tttatctata
      181 attcctgcaa aaaccgatcc tataagaaag cctgccagag gcatagcagc ctgaaatata
      241 ccgaaataca gccccacaat caaggcatct ttccgggtta ctttcttaag tgaaagacct
      301 ttacatatag ctaccgcaaa agcatccata gaaagtccta ccgctataag cagcagttcc
      361 aaaaaagtca tgtcgctctc ctaacagttt cgttttatta tataatcgag tgatggagaa
      421 ggcaataaat ttttgcatat attcaacgaa ccgcattgtt tttaacggct ctgtatgata
      481 aagtgtaagt atcacaaata gaaggagaca tatatggacg cactatttcc gctgaataaa
      541 aatgttaaga agaaaaaact cagcactttg gtaatagcaa ttttgcttta tgtagtttta
      601 tcggttattg taggactgat tcaatggctc ttacgcggga ttcccgtcgt aaataccgtt
      661 atgaatatag tcggatattt ggtttggctg tacagtgtgg taggcgtgat tctggctatc
      721 tttaaattcg ttaagtagcc ggaatgtaac atacttagtt tactataatt gtgttttgcg
      781 tatgcacgac tatactttga ttcgttcaaa gagaagaact atatcattaa gaatctcaca
      841 ggattgtacc gttatagtac gggcaccttt tttgtgttca aaaaaagtta ttgataaatt
      901 tgtatccgat cataaaaaat ggatagacag gcaaatagaa attagaaaaa atcagccttc
      961 agtgaaaaac ctaacaagcg aacagattaa gaagttaaag aagcaggcta agcaggtgct
     1021 caccgaaaga acaaagtatt attcggaaat aacgggactg tatcctgcat atattaaaat
     1081 aacatcctcg gaaaagcgct tcgggagctg cactcaaaaa ggcggaatat gttattcata
     1141 cagacttatc ttgtatccgg aaagagctgt ggattatgtg atagtgcatg aactgtgtca
     1201 tttgcggcac cggcaccatc agaagccttt ttatgacctt gtaaagaaat atatgccgga
     1261 ttataaggag agggataaac ttcttaaaaa cgtaaccggt atgctttagt aagttactct
     1321 aaagtagcat atgctttttg catgaaatca tccatgtatg tgatttcggt aatccagtaa
     1381 ttaactatta tttcatccac atttttggat ttggcaaagt ctattatatt catgttgccg
     1441 cagtatgcct tgttgtatgt acgggaatcc actacatata tagtcttgta gtgaggtaac
     1501 aggaaggata ttaaagaatc tccgaaagag tcttttataa ccatacactt aagatcggag
     1561 tcggacgatg tggttatttc gctgtatgtt ccgaaacccc cgtcggaaaa cagcaggtag
     1621 taatctttgt cgggtttgac cctttctttg tttataagtc tcttttcaat tgaaacatcc
     1681 tgactgttgt aaatgtattt gtaatctact atcggatagt atgcgattat ggtatcggga
     1741 ttatcataat cggcctgtgt aagatagggt ttattgtaaa acccgagata accttccaca
     1801 acaagttttt cactgtagtt gcttaaagga attatttccg attgcctgtt tcttgccttc
     1861 ataagttccg tatagccgta ataagcaccc aggtgtgtcc agtgatgatc cgtacggtaa
     1921 tatatgtatt catcaacatg ctcgaccatg gaccgcaata cgtttacatt ttttacggac
     1981 ttatcaatct tgctgtttat ctgttcgaca tttgcagcac gggaacttaa gaaatcatat
     2041 tttgttaatg caagataggt ggagcgggac ggtgccagca tactgtatac ttcaatatcc
     2101 ggtccggcca tcttctttat tttatttatg taattcgcgt agttgtcata tgcctcttcc
     2161 gaat
//
LOCUS       DAOPWC010000280         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54666_length_2158_cov_4.049453, whole genome shotgun sequence.
ACCESSION   DAOPWC010000280 DAOPWC010000000
VERSION     DAOPWC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54666_length_2158_cov_4.049453"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PKJ65_06135"
     CDS             complement(<1..1245)
                     /locus_tag="PKJ65_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ41448.1"
                     /translation="MKKICITGLLKEKKPMLSFLQKRGVVDIQDMPIDSEYENVFTKG
                     YDKNSEAEIDSKMALLSKAISVLEEYDESKKPLFKVRKDIDRQYYESITQYNDEFIGL
                     AEKVIETHNSIGDFRDEINRLENDILTLTPWTDLDIPINMSKTDKVNIMLGTIPEFVK
                     PETYTQAIDEKGLLCQVQEISKDKNNAYVAIYIHESDMREPMHYLKSIDFNILEYDFS
                     GIPGRLIEENKQTISRLKVNIENAKSSLKEYALKTGEVQAAYDHFAISKGLLTVDDKI
                     LSGKYSFIIDGWIPEEKVEKTVGALEKDFVCHVFVRDPHDDEEFPVLLKNGFLGDSVT
                     GVTEMFSLPNCREADPNTVTAIFFALFFGMMLSDAGYGLVMSVVCAVILLKFKLEYAT
                     ARFIKLMLISGIATVVVGALYGS"
     gene            complement(1247..1570)
                     /locus_tag="PKJ65_06140"
     CDS             complement(1247..1570)
                     /locus_tag="PKJ65_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41449.1"
                     /translation="MMEKTYLDKIKEAEEKAAGIFRDAVNKSELMLGDAEKEHSEYER
                     KSIEKIDEQCKNMLLKAEQEAADLVQKADREYAKMSEDLKTEALSNVKEAVDFVLEKV
                     TNGSR"
     gene            complement(1679..>2158)
                     /locus_tag="PKJ65_06145"
     CDS             complement(1679..>2158)
                     /locus_tag="PKJ65_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ41450.1"
                     /translation="LYLTANQEAYSLNYRSSARVGKTAKRHLVDPSLCCASLQLTKDK
                     LLNDHETFGLMFEALVKRDLRIYADYLDGYLYHFRDNSSGDEVDAIIEFSEGNYAAFE
                     IKLSDRGIKDAIESLSRFYTNVKRKPVFMCVIVGHCEVIMKDRKTGIYIVPITSLKP"
BASE COUNT          587 a          503 c          351 g          717 t
ORIGIN      
        1 ggaaccgtat aaggctccca caacaactgt tgctatgccc gatataagca tcaactttat
       61 aaaccttgcc gtggcgtatt ccaacttgaa tttgagcagt ataaccgcac agactaccga
      121 cataaccagc ccgtaaccgg catcagaaag catcatcccg aagaacagtg caaagaatat
      181 tgccgtgacc gtattgggat cggcttccct gcaattgggc agactgaaca tttccgtaac
      241 tcccgttacc gagtctccta agaatccgtt cttaagaagt accggaaact cctcatcatc
      301 atgaggatct cttacaaata catgacaaac aaagtctttt tccagagcac cgaccgtctt
      361 ttcgactttc tcttccggta tccagccgtc gattataaag ctgtatttgc cggaaagtat
      421 tttatcgtcc actgtcaaca atcctttgct tatggcaaaa tggtcatagg cagcctgtac
      481 ttctcctgtc ttcaaagcat attctttcaa actgctcttt gcattctcta tatttacttt
      541 aagtcgggat atagtttgtt tattctcttc aattaaccga ccgggaatac cggaaaaatc
      601 atattccaat atattaaagt ctatactttt aagataatgc atgggctctc gcatatccga
      661 ttcatggata tatatcgcca cataagcatt attcttatcc ttgcttatct cctgaacctg
      721 acataataat cccttttcat caattgcctg agtatatgtc tcgggtttta caaactccgg
      781 tattgtaccg agcataatat tgactttatc ggttttagac atgtttatgg gtatatcaag
      841 atcagtccac ggcgtaagag tcagtatatc attttccagc cggtttattt catctctgaa
      901 atccccaata gagttgtgag tctctataac cttctcggca agtccgataa actcatcgtt
      961 gtattgcgtt atactttcat aatattgtct gtctatatct tttctgactt tgaataaagg
     1021 ttttttggat tcatcatatt cttccagaac ggatatggct tttgacagca aagccatttt
     1081 gctgtctatt tcggcttcgg aatttttgtc ataacctttg gtaaagacat tctcatactc
     1141 cgaatcaatc ggcatatcct gaatgtccac aacgccgcgc ttctgaagga aagaaagcat
     1201 gggctttttc tcctttaaaa ggccggttat gcaaatcttc ttcatgctaa cgactgccat
     1261 ttgtcacctt ttccaaaaca aaatccaccg cttctttaac attggaaaga gcttcggttt
     1321 tcaagtcttc actcattttt gcatattccc tgtccgcttt ttgcacaagg tccgctgctt
     1381 cctgctctgc tttcaagagc atgtttttgc actgttcatc aatcttttca atgctttttc
     1441 tctcatactc cgagtgctct ttctccgcat caccaagcat caattccgac ttattgacgg
     1501 catctctgaa aatacccgcc gccttctctt ccgcttcttt gattttatct aaatatgttt
     1561 tttccatcat ctttcccatc tgttaatcac atcaacatat tttttattca acaaaagaca
     1621 ttatatcata ttaatatatt gaattgcaga gagaatataa ataaattata tttaagtctt
     1681 acggctttag tgaagttatg ggaacaatat atatgcccgt tttccgatct ttcattataa
     1741 cttcacagtg tccgacaatg acacacatga atacgggttt cctttttaca ttggtataaa
     1801 atcttgaaag gctttctata gcatccttaa tacctctgtc gctcaatttt atctcaaaag
     1861 ctgcataatt accttcactg aactctataa ttgcatccac ctcatcgccc gacgagttgt
     1921 cacgaaaatg atagagataa ccgtcaagat aatctgcata tattctcaag tcccgcttta
     1981 caagtgcttc aaacatcaga ccgaatgttt cgtggtcatt aagcaattta tctttcgtaa
     2041 gttgcaggct tgcacagcaa agtgacgggt caactaaatg tctcttagcg gtttttccga
     2101 cccgtgcgga agaacgataa ttcagactgt aggcctcctg gtttgcagtg agatataa
//
LOCUS       DAOPWC010000281         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54798_length_2155_cov_2.248095, whole genome shotgun sequence.
ACCESSION   DAOPWC010000281 DAOPWC010000000
VERSION     DAOPWC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54798_length_2155_cov_2.248095"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PKJ65_06150"
     CDS             <1..1087
                     /locus_tag="PKJ65_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ41451.1"
                     /translation="NDIGYWAMLPSLAKDQTQRATLTSLANLFAFVGAIIAFATIPIL
                     TNGPNAIGGNSITGYMWVSIIIAILFVAFQLLTAIFVQEAPVSYRSDDEKIGLKAMFQ
                     VVFKNDQLLWTSLVMLLYNLGSSLINVFGIIYVYMYFGYDGINITLFVGAYALGNLMI
                     NIVYPQLVKVMTRRQIATMGLVGVVIGYGFFFIVGVLIPMNYLLLCVAGFVLSFGQAM
                     IYMVTTINLTNCIEYNEYKTGNRDEAIIFSIRPFMAKMSSALQQGIVGTAYLALGITA
                     ITNGISEAERLSNMGVISTEEKSTMIEQALALGTDSMKMNFRIFIVVVPIILISLAYL
                     VMMKKNKIDENAYNRMLLEISQRKQAE"
     gene            1300..>2155
                     /locus_tag="PKJ65_06155"
     CDS             1300..>2155
                     /locus_tag="PKJ65_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNZ41452.1"
                     /translation="MQKDTNPVLQFSDKSNLLEQSAYKYQLQDRETPNLYRYLYDYKT
                     VPKVPFNHRSVPMNMPKEIWITDTTFRDGQQSTSPFSVEQIVHLFKLMHKLGGPKGLI
                     RQSEFFVYTEKDRKAVEECMSLGYKFPEVTSWIRANEKDFDIVKSLGLKETGILVSCS
                     DYHIYNKMGLTRKQAMDKYLGIVKSVLDKGIKPRCHFEDITRADFYGFVVPLAYELTK
                     LSEETKIPIKIRACDTMGFGVSYPGAALPRSVQGIIYGIYHYGGVKSELIEWHGHNDF
                     YKVVTNAST"
BASE COUNT          686 a          360 c          499 g          610 t
ORIGIN      
        1 gaacgatata ggatactggg caatgcttcc gtctttggct aaagatcaga cacaaagagc
       61 tacattgacg tcactggcaa acctgtttgc atttgtcgga gcaattattg cttttgccac
      121 aatacccatt ctgacaaacg gccccaatgc aataggcggc aactccatta cgggatatat
      181 gtgggtatcc attattattg cgatactttt tgtggccttc cagctgttga cggcaatctt
      241 tgttcaagag gcaccggtct cctatcgcag tgatgacgag aaaatagggc ttaaagcaat
      301 gtttcaggtg gttttcaaaa atgaccagtt gctgtggact tcgcttgtaa tgttgctgta
      361 caatctcggg tcttcactta ttaatgtttt cggtatcatt tatgtttata tgtatttcgg
      421 ttatgacgga ataaatataa cattgtttgt gggggcttat gctttaggaa atctgatgat
      481 aaacatagta tatccgcagt tggttaaggt catgacaaga agacagatag caactatggg
      541 acttgtggga gttgttatcg gctacggatt cttctttata gtgggtgtgc tgattcccat
      601 gaattatctg cttttgtgcg tagcaggatt tgttctgtca ttcggtcagg cgatgatata
      661 tatggtaaca accataaatc ttaccaactg tattgaatac aatgagtata agacaggtaa
      721 ccgtgatgaa gccatcatat tctccattcg tccttttatg gctaaaatga gttctgcact
      781 ccagcagggt attgtcggta ccgcatacct ggcgctgggc ataacggcta ttaccaacgg
      841 tatatcggaa gctgagagat tgagcaatat gggcgttatc agtactgaag agaagagcac
      901 gatgatagaa caggcattgg cgctcggaac cgattccatg aaaatgaatt tcagaatatt
      961 cattgtggta gttccgataa tattgatttc gcttgcatat ctggtaatga tgaaaaagaa
     1021 taagattgac gaaaatgcat ataaccggat gttgttggag ataagccaaa ggaaacaggc
     1081 cgaatagact taaagaacat atattacaaa acagcttccg taacggaggc tgtttttgtt
     1141 gacatcggat aaatatgtaa aatgtagata aataagtaaa aatgaaagta aaaacatgtt
     1201 aatactgcaa ttattatatt gcaattatgc aaaaatgatt atataatgcg gaataattta
     1261 tgctgattag catattaatg aaaggaatag aaataaatta tgcaaaaaga cacaaatccg
     1321 gtacttcagt tctccgacaa gagcaacctt ttggaacagt ccgcatataa gtatcagttg
     1381 caggacaggg aaacacctaa tttatacaga tatctttatg attataaaac ggtacctaaa
     1441 gtacctttta atcacagaag cgtacccatg aatatgccca aggaaatatg gataaccgat
     1501 accacattca gagacgggca gcagtccact tcaccgttca gtgtggagca gattgtgcat
     1561 ctttttaagt taatgcataa gttgggagga cctaagggtc ttataaggca aagcgagttc
     1621 ttcgtttaca cggaaaaaga tcgtaaggcg gtagaagaat gtatgagtct cgggtataag
     1681 tttccggaag taacaagctg gataagagcc aatgaaaagg attttgacat tgtaaaaagt
     1741 ctcggtttaa aagagaccgg aatattggtg tcctgctccg attaccatat atataataag
     1801 atggggctta ccagaaaaca ggcgatggat aagtatctgg ggattgtaaa gagtgttctg
     1861 gataaaggaa ttaagcccag atgccatttt gaagacatca caagggcgga cttttacggg
     1921 tttgtcgtgc ctttggcata tgagctgaca aagttgtcgg aagaaaccaa aatacccata
     1981 aagataagag catgtgatac catgggattc ggtgtgagtt atccgggtgc tgcattaccg
     2041 agaagcgtgc aggggataat atacggaatt tatcactatg gcggagttaa gagcgaactt
     2101 atagagtggc acggtcataa tgatttctat aaagtagtaa ccaatgcatc aacgg
//
LOCUS       DAOPWC010000282         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54928_length_2151_cov_2.899809, whole genome shotgun sequence.
ACCESSION   DAOPWC010000282 DAOPWC010000000
VERSION     DAOPWC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54928_length_2151_cov_2.899809"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKJ65_06160"
     CDS             <1..824
                     /locus_tag="PKJ65_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ41453.1"
                     /translation="TTMGHAGFEEGYGDYIDNKHEAFTDIEEIYNLDFYETYGTIDLK
                     SKAKAITEGYLERCQLHPDGVNTSGIYVTCISGILEILGWDMLLSAAGEDPARFGEFT
                     VRYSQWISQYFKALAKSDVPVCMIHDDMVWSSGPFIAPAWYDKYVFPYVKKNISYLKE
                     AGKKVLFTCDGGFDLFVDRFVDMGVDCLIFEPLTDLKYICERYGKTVSIIGNADTRII
                     MKGSREDIYDEVRRCMDLGKPCPGYMLAIGNHITPGHNVENYLYYNEVYEKLKRR"
     gene            934..1503
                     /locus_tag="PKJ65_06165"
     CDS             934..1503
                     /locus_tag="PKJ65_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ41454.1"
                     /translation="MSLVINKTREILLEKCRQHKEHVGYDYWNDHIKKVVEHALYLAD
                     LYGADKEIVELGALLHDISMPAEYGSRSAHYVYSADMAEQLLTDLNYPQNKIERVVKC
                     VYNHSGRNQHLRTTIEEHCVADGDALAHFDRIPSLFSLAYNLHGMSLEEGREYVKKRL
                     QNDFSELSEQTKEMFRKKYETIMEVVFVE"
     gene            1588..>2151
                     /locus_tag="PKJ65_06170"
     CDS             1588..>2151
                     /locus_tag="PKJ65_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNZ41455.1"
                     /translation="MKTTPLTSVHESLRAKMVDFGGFYMPVQYTSIIEEHNAVRSKAG
                     IFDVSHMGEISVTGKDAIKYLNYIITNDIGAISENQIMYSPMCYEHGGCVDDILIYKN
                     NDKDFLVVANASNTDKDYDWMKKHTKGYDVVVSNQSDKYGQIALQGPEAQSILQQLTK
                     TDLEEIAFYRFKNNVVVDGVNTMVSRTG"
BASE COUNT          723 a          331 c          507 g          590 t
ORIGIN      
        1 ggaccactat ggggcatgcg ggatttgaag aaggttacgg ggattatata gataacaagc
       61 atgaagcctt taccgatatt gaggaaatat ataatctgga tttttatgag acttacggaa
      121 caattgattt aaaatccaag gcaaaagcga taaccgaggg ttatcttgaa agatgtcaat
      181 tgcatcctga cggagttaat acttcaggta tatatgtcac atgtatatcg ggaattttag
      241 agatactggg atgggatatg ctgctgtcgg ctgccgggga agatcctgcc cgtttcggtg
      301 agtttaccgt aagatattcc caatggattt cacagtactt taaggcgctg gccaaatccg
      361 atgttcccgt atgtatgata catgatgata tggtatggtc atcgggacct ttcatagctc
      421 ctgcctggta tgacaaatat gtattccctt atgttaagaa aaatatctct tatttgaaag
      481 aagcgggcaa aaaggtctta tttacctgtg acggcgggtt tgacttgttt gttgataggt
      541 ttgtggatat gggggttgac tgtttaatat ttgaacctct tacggattta aaatatattt
      601 gtgaaagata cgggaaaacc gtttccatta tcggaaatgc cgacacgaga ataataatga
      661 aggggagcag agaagatatt tatgatgagg taagacggtg catggatttg ggtaagccct
      721 gccccggata catgcttgca ataggtaatc acataactcc cgggcacaat gtcgagaatt
      781 acctttacta taatgaagta tacgagaaac tgaaaagaag ataaaaagct gagaatggct
      841 gaatgagaca cttgctaaaa tgcatcccgg tggacataaa tgaattaata aagtgaagag
      901 agaaaataaa taaggataaa aaggaaatat gccatgagtt tagtaataaa caaaacaaga
      961 gaaatactgt tggaaaagtg tagacaacac aaagagcatg tcggatatga ttactggaac
     1021 gatcacataa aaaaagtggt tgaacatgca ctttatttag ccgatttgta cggagccgat
     1081 aaagaaattg tggagttggg tgcacttctc catgatatct ccatgcctgc tgagtatggt
     1141 agtagaagcg cgcactatgt ttacagtgcg gatatggcgg agcaactgtt gacggattta
     1201 aattatcctc aaaataaaat agaaagagtg gtaaaatgcg tttacaatca tagcggcaga
     1261 aatcaacact taagaacaac catagaagaa cattgtgtag cggatggaga tgcacttgct
     1321 cattttgacc gtattccgtc tcttttcagt ttggcatata atctccatgg gatgagtctg
     1381 gaagaaggca gggagtacgt gaaaaaacgg ttacaaaatg atttttctga attaagcgaa
     1441 caaactaaag aaatgttcag aaagaaatat gaaactatta tggaagtagt atttgtagag
     1501 tagatgttgt gatgttatac atatgaccgt tgacttttct tcttttaaaa catattataa
     1561 atatatattt caaggagcat ataacttatg aaaacaacac ctttaacctc tgtgcatgaa
     1621 tctttgagag ccaaaatggt agacttcggg ggcttctata tgcctgtgca gtatacctcc
     1681 atcatagagg agcacaatgc agtaagaagc aaggcgggta tttttgatgt ctcgcatatg
     1741 ggagagattt cggtaacggg aaaagatgcg ataaaatatt tgaactatat tataaccaac
     1801 gacatcggtg ccatatcgga aaatcagata atgtattcac ccatgtgcta tgagcatgga
     1861 ggatgtgtgg acgatatcct gatatacaag aacaatgata aagactttct tgtggttgca
     1921 aatgcgtcca ataccgataa agattatgac tggatgaaaa aacatacaaa agggtatgat
     1981 gtggttgtca gtaaccaatc ggataaatac ggacagattg cactgcaggg acctgaagcg
     2041 caaagtattt tgcagcaatt gactaagacc gatctggaag agattgcatt ttacagattt
     2101 aagaataatg tggttgtgga cggcgtgaac accatggtat ccagaacggg c
//
LOCUS       DAOPWC010000283         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54989_length_2149_cov_3.762178, whole genome shotgun sequence.
ACCESSION   DAOPWC010000283 DAOPWC010000000
VERSION     DAOPWC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54989_length_2149_cov_3.762178"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..468
                     /locus_tag="PKJ65_06175"
     CDS             61..468
                     /locus_tag="PKJ65_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNZ41456.1"
                     /translation="MYCAFASLGFAAVENILYIVNFQAENPSIALYRGIFSVPGHMLF
                     AVTMGYYISLSKFAPTYALMRRYKVKSLLYPVLLHGTFNFILSLNIHWLFAFVPFVIF
                     LWIFNIKKLRSFSKESRYYKNLYDNNNPDNTSI"
     gene            complement(531..866)
                     /locus_tag="PKJ65_06180"
     CDS             complement(531..866)
                     /locus_tag="PKJ65_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41457.1"
                     /translation="MARKREQYVLAVKNLDKTLADIAAGKYKMPVENSKYAEIFATIE
                     RRCNNLDELPRFIRKAKMKKSECIHWWEGIIDDGYELLIVQYNAPDENFVELAGSEDV
                     IKFVVSVKK"
     gene            complement(979..>2149)
                     /locus_tag="PKJ65_06185"
     CDS             complement(979..>2149)
                     /locus_tag="PKJ65_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HNZ41458.1"
                     /translation="QALELGQQVMKFINEQSKKASMDLAQIKGVFPAYDKSIYPAQDI
                     KVRNATTTTIAPTGTLSIIAGVSSGIEPVFAISYIRNIMDNTEMVETNPVFEEVARSR
                     GFYSEELMRDIAKKGSVKYMTDVPEDVKEVFVTSHDIDPSWHVHMQATFQEHVDNAVS
                     KTVNLRNEATLDDVREVFMQAYAMGCKGVTLYRDGSRAAQVLNIPKQQHKETVQSGTI
                     LIPKQRPRITTGLTEKFRTGCGNLYITVNYDENGEICEIFTNTGRAGGCPSQSEAVAR
                     LVSIALRSGIDTEAIIDQLKGIRCPSTIRQKDLGILSCPDAIGRMIEKVAKMNGELKE
                     EVKRTIPYLHYGETKNSTDKNEKSFEGRRCPECKAPLESDGGCMICRSCGYSKCN"
BASE COUNT          521 a          496 c          369 g          763 t
ORIGIN      
        1 tcttcttgtt ttattaactt ttaaatttat tttccccaaa atactttaca ctaaggaatt
       61 atatactgtg cttttgcgtc tttgggcttt gcggcggtgg agaatatttt atatatagtg
      121 aatttccagg cggaaaatcc ttcgatagcc ctttacagag gaattttctc cgttccgggg
      181 cacatgcttt ttgcggttac aatgggatat tacatatcgt tatccaaatt cgctccgact
      241 tatgctctga tgagacgtta caaagtaaag tcgttattgt atcccgttct gcttcacggc
      301 acattcaact ttattctttc gttgaatatt cactggcttt ttgcatttgt tccttttgta
      361 atattcttat ggatatttaa cataaaaaaa ttgcgttcct tctcaaaaga aagtcgctat
      421 tacaaaaact tatacgacaa caataaccct gataacacaa gtatttaact tatttttgcg
      481 cgaaaaaaag catcagatgt ttgatgcttt ttttatttat tcagatactt ctacttttta
      541 acggatacaa cgaacttaat tacgtcttcg cttcctgcca attcaacaaa gttctcgtcc
      601 ggagcattgt attgaactat cagaagttca tatccgtcgt caataatgcc ttcccaccag
      661 tggatgcatt cgctcttctt catcttagcc ttcctgataa atctgggtaa ttcatcaaga
      721 ttattgcatc ttctctctat cgttgcgaaa atttccgcat atttggaatt ttcaaccggc
      781 atcttatact tgcctgcagc tatatcagcc aacgttttat ctaaattctt tactgccaat
      841 acatattgtt ctctttttct tgccatgtat agatcctcct attgctgtca gtcttaatta
      901 attatatcta ttttagccaa atatgtccat aaaaatattc cataaattta ggcatatcta
      961 tttgtatgat ttatacagtt agttgcattt tgagtatccg caggaccgac aaatcatgca
     1021 tccgccgtca ctttccagcg gagctttgca ttcgggacag cgcctgccct caaagctctt
     1081 ctcgttttta tccgtggaat tcttagtttc tccgtaatgc aggtagggaa tggtcctttt
     1141 tacttcctct ttaagttcgc cgttcatttt ggcaactttt tctatcatcc tgcctatcgc
     1201 atccggacag gacaatattc ccagatcctt ctgccttatg gttgagggac acctgatacc
     1261 ttttaactgg tcaataatag cttcggtatc tatacccgat ctcaatgcaa tagacaccag
     1321 ccttgcaacc gcttccgact gtgaaggaca tcctcccgct cttccggtat tggtgaatat
     1381 ctcgcatatt tctccgtttt cgtcataatt gacggttatg tacagattgc cgcatcccgt
     1441 acggaatttt tccgtcaatc ccgtggtaat tctcggacgc tgtttgggta tcagtatcgt
     1501 accggactga accgtctctt tgtgttgttg tttcggtata ttcagtacct gtgcggctct
     1561 gcttccgtct ctgtacaagg taactccctt acatcccatt gcatatgcct gcataaacac
     1621 ttctcttaca tcgtccagtg tcgcttcatt ccgcaaatta accgttttgg aaaccgcatt
     1681 gtccacatgc tcctgaaaag tcgcctgcat atgtacatgc catgaaggat ctatatcatg
     1741 actggtcaca aagacttcct taacatcctc cggaacatcg gtcatatatt taaccgagcc
     1801 tttttttgca atatctctca tcagttcttc ggagtagaaa cctctgcttc ttgccacttc
     1861 ttcgaacacg gggtttgttt ccaccatttc ggtattgtcc attatattac gtatgtatga
     1921 tatggcaaaa accggttcaa ttccgctgga tacgcctgct attatgctga gagtgccggt
     1981 aggagctatt gtggtggttg tagcatttct tactttaata tcctgtgcgg gataaatact
     2041 tttatcatat gccggaaata ctcccttaat ctgtgcaagg tccattgagg cttttttgga
     2101 ttgctcgttt ataaacttca ttacttgctg tcccagttcc aaagcctgc
//
LOCUS       DAOPWC010000284         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_54998_length_2149_cov_2.992837, whole genome shotgun sequence.
ACCESSION   DAOPWC010000284 DAOPWC010000000
VERSION     DAOPWC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54998_length_2149_cov_2.992837"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKJ65_06190"
     CDS             complement(<1..219)
                     /locus_tag="PKJ65_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41459.1"
                     /translation="MRKSISFKSAYLVPAMIIIMFLLLFVMLFQMRFSITEFVMEQAA
                     SDNEMIAKEKSALFDSLLENMSLETRGIL"
     gene            complement(228..878)
                     /locus_tag="PKJ65_06195"
     CDS             complement(228..878)
                     /locus_tag="PKJ65_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41460.1"
                     /translation="MSQPKNTENVIEEQDHLIQEEEVKPEEKADAVEEQDVKKEKKGL
                     LSRFRRPQEQDIYDDTDYQGMLRKNFYDFDQISKKRQITSLRMAVMALTVILIVILTL
                     YIVHLSDSTILLDKLIESSSKYVYDNVLYVDNQIKYTEVVSELNVMRTLMNHNGELGK
                     RQYAISELYYIAIQLPDSFIKHRTEVYDAFKNINEDKQELGYKLIEELLIKIVEGR"
     gene            complement(865..1545)
                     /locus_tag="PKJ65_06200"
     CDS             complement(865..1545)
                     /locus_tag="PKJ65_06200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ41461.1"
                     /translation="MKKFFIKKNARRIFTVVFSVLLVLLVLLVGNIIYSQITHKVPAI
                     FGYSVMNIIAGSMEPVIPKNTFILIKQVDPEQLQVGDVITFYSSDPLIQGLPNTHRIF
                     DIVVENGQKFFVTKGDANAIPDSYNVGFDDVIGVYVRNIISLGKIGLIFQNKSIVFVL
                     LVVPAAVLFIMEIINLTKTAAKVKQEDIESVSNEDKCDDVESADSNETDSEHKKSEEK
                     EEKSIEST"
     gene            complement(1586..>2149)
                     /locus_tag="PKJ65_06205"
     CDS             complement(1586..>2149)
                     /locus_tag="PKJ65_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41462.1"
                     /translation="RVLRIRKIIRQYQASPFITQMGKAYIRPPVMRTNAITKNVHLRE
                     CLELWLFIESYESAGFDIESSSVTEKPGKELMRNLYRLLAFQYVIFRKNTYDQLDDII
                     NKSTAKLRYNARFKQMNIEEFTDQYEFLDPGVKKVYVQELSRKGTRLTKEELKIRKAI
                     ENALKSERAYEAEKKKAVLPKRKKKGQ"
BASE COUNT          603 a          499 c          324 g          723 t
ORIGIN      
        1 caatattcct cttgtttcca aagacatgtt ctccagtaaa gaatcgaata aagcggactt
       61 ttcctttgca atcatctcat tatcgcttgc cgcctgttcc attacaaact ccgtaatgga
      121 aaatcgcatc tggaacaaca ttacaaacag cagaagaaac atgatgatta tcattgcagg
      181 gacaaggtat gcacttttga agcttatact ttttctcaac ttacttgcta tcttccctcc
      241 actattttaa tcagaagttc ctcgataagc ttatacccca actcctgttt gtcttcatta
      301 atgttcttaa acgcatcgta tacttccgtc ctgtgtttaa taaaagaatc gggaagttgg
      361 attgctatat agtataactc gcttattgca tattgtcttt ttcccagttc cccgttatga
      421 ttcatcaaag tcctcataac attaagttcg gaaaccacct cggtatattt aatctggtta
      481 tccacataca atacattatc ataaacatat ttacttgacg actcaatcag tttatccaac
      541 agaattgtcg aatcgctcag atgcactatg taaagtgtca atattacaat cagaataacg
      601 gtaagagcca ttaccgccat ccgcaatgaa gttatctgcc gctttttgct tatctggtcg
      661 aaatcgtaaa agtttttcct gagcataccc tgataatcgg tatcatcgta aatgtcttgc
      721 tcttgcggac gacgaaatct gcttaataag cctttctttt ctttcttaac atcctgttct
      781 tcaaccgcat cagctttttc ctcaggcttt acttcttctt cctgtatcaa atgatcttgc
      841 tcttcaatca cattttccgt attcttaggt tgactcaatg ctcttttcct ccttttcttc
      901 cgatttttta tgctccgaat ccgtttcatt tgagtcggct gattcaacat catcacactt
      961 atcttcattt gaaaccgact caatatcctc ctgctttacc ttggcagctg ttttggtaag
     1021 attgataatt tccattataa acagcactgc cgccggtaca acaagaagca cgaatacaat
     1081 tgatttgttc tggaaaataa ggcctatttt acccagtgaa ataatattcc tgacataaac
     1141 ccctataaca tcatcaaaac cgacattgta ggaatcagga atggcattgg catctccctt
     1201 tgttacaaaa aacttctgac cgttctcgac aacaatgtca aaaattctgt gggtattggg
     1261 aagtccctgt ataagcggat cggaagaata aaatgtaatc acatccccca cctgcaattg
     1321 ttcggggtcc acctgcttta ttaatatgaa agtatttttc ggtataaccg gttccatact
     1381 gccggcaatg atattcatta cactgtatcc gaaaatagcg ggaactttat gagttatctg
     1441 tgaataaatg atattaccca ccagcagaac cagcaaaacc agtaaaacgg aaaatactac
     1501 cgtgaaaatt cttcttgcat tttttttaat aaaaaacttc ttcatatcag tatatgtacc
     1561 tcaaatccgt aatgcgtaaa aatacctact gccctttctt tttacgtttc ggcaaaacag
     1621 ccttcttctt ttccgcctca tatgctcttt ccgatttcag cgcattttcg atagctttac
     1681 ggatcttcaa ttcttcttta gtcaatctgg tgccttttct gctcaactcc tgaacatata
     1741 ctttcttgac accgggatct aaaaactcat attgatcggt aaactcttca atattcatct
     1801 gtttaaaccg ggcattgtat ctgagctttg cggtactctt attgattatg tcatccaact
     1861 gatcgtatgt gttttttctg aagataacat actgaaaagc cagcagcctg tagagattcc
     1921 gcataagttc tttgccgggt ttttccgtta ccgaactgct ttctatatca aagcccgcac
     1981 tttcgtaact ctcaataaac aaccacaatt ccagacattc cctcaaatgc acattcttgg
     2041 taattgcatt ggttctcatt acgggaggac gtatatatgc ctttcccatc tgagttataa
     2101 agggtgaagc ctgatattgc cttataatct tccttattct caaaaccct
//
LOCUS       DAOPWC010000285         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_55123_length_2146_cov_2.911526, whole genome shotgun sequence.
ACCESSION   DAOPWC010000285 DAOPWC010000000
VERSION     DAOPWC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55123_length_2146_cov_2.911526"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1495
                     /locus_tag="PKJ65_06210"
     CDS             167..1495
                     /locus_tag="PKJ65_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ41463.1"
                     /translation="MYLKRKIDSFLEEWKANPDRMPLIVKGPRQVGKTQSVIQFGNVN
                     YKSVVYINFVEEPKYKLITTDGYSAEGIIKNISRMDPSKQFIGGETLIIFDELQEFPD
                     IATSLKFFKIDGRFDVICSGSMLGINYHKIESNSVGYKSDYEMYSMDFEEFLWAKGYN
                     DGHIESMLENMKNLTPFNEVEMSVYNNLFLDFCILGGMPAIVREFIKKGTFEGSLELQ
                     RQLLIDYKEDIRKYAVGMDQTRILNVFNHIPIQLAKDNKKFQISKVASGARFRDYRGC
                     IEWLDDAGIINLCYCLNFPELPLRGNYDDTKYKIYFADTGMLVAMLDEEAQEDMRANR
                     NLGVYKGALYENIVGEALKKSGYSLYYYKRNDSTLEQDFFVRTAKSLIPIEVKAKSGT
                     AKSMRMLIGEDRYSDISYGIKLTGGNIGYSEEIYTFPYFCTFLLKRYLSD"
     gene            1575..>2146
                     /locus_tag="PKJ65_06215"
     CDS             1575..>2146
                     /locus_tag="PKJ65_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41464.1"
                     /translation="MDYKRTDEIIKRTKDMYESGKGALIQVKEIFSGIDVPFRPLDSF
                     GLPKNMETYLDYMIERQRIFWNIRNQISDDIIQSITVRYGIAEHSAFMGGDVTFSETT
                     SWHHQHVKDYSDYTLAKVDESNIWRNLVIEGMKYMKSKVKDEFFIRFRGADGPLDVVN
                     ALRGNDIFYDFYDEEEGLLELTSKCTDAII"
BASE COUNT          720 a          324 c          480 g          622 t
ORIGIN      
        1 atcggaagac agattttagc acttctttct aaaaatgtgg gaagattgct aaaaactccc
       61 tcatgaaatg tgcagaaaag ctaaaaactc cctcattttt tgtgaaagcg gttgaatttc
      121 agttattttg cggatataat tgtggagaga gatagtgagt gaaatgattt atctgaaaag
      181 aaaaatagac agctttctgg aagaatggaa ggcaaatcct gacagaatgc ctttgattgt
      241 taaaggaccg cgtcaggtag gaaagaccca atccgttata caattcggga atgttaatta
      301 taaaagtgtt gtttatatta actttgtaga agaaccgaaa tacaaactga ttacaacgga
      361 cggatatagc gcggaaggta ttataaaaaa tatttccaga atggatccgt ctaaacaatt
      421 tattggagga gaaactttaa taatattcga cgagttgcag gaatttcccg atatcgcgac
      481 atcattaaag ttttttaaaa tcgacggaag atttgatgtt atatgcagcg gatctatgct
      541 cggaattaac tatcataaaa ttgaaagtaa cagtgtaggc tataaatccg actatgaaat
      601 gtactctatg gattttgaag aatttctttg ggcaaaggga tataatgatg gtcatattga
      661 aagtatgctg gaaaatatga aaaatctcac accgttcaat gaggtggaaa tgtcggttta
      721 caacaatctt tttctggatt tttgtatatt gggaggaatg cctgcgattg ttcgtgaatt
      781 tattaaaaaa ggaacatttg aaggttcact ggaacttcaa agacagcttc tcattgacta
      841 taaagaagat atccgaaaat atgccgtagg tatggatcag acaagaatat tgaatgtgtt
      901 caatcatatt cccattcagc ttgctaaaga taataaaaag tttcagatat ctaaagttgc
      961 ttccggcgca cgttttcgcg attatcgcgg ctgcattgaa tggttagacg atgcgggaat
     1021 tataaatctc tgctattgcc tgaattttcc cgaattaccg ctgcgtggaa actacgatga
     1081 tacaaagtat aagatttact ttgcagatac cggaatgctt gtagcaatgc ttgacgaaga
     1141 agctcaggaa gatatgagag ctaacagaaa cttaggagtg tacaaaggtg cactgtatga
     1201 aaatatagtg ggggaagcat tgaaaaaatc cggatacagt ctctactatt acaagcggaa
     1261 cgattccact ttggagcagg atttctttgt aaggacggca aaatctctta tcccgataga
     1321 ggtaaaggcg aaaagcggaa ctgccaaatc aatgagaatg ctgattggcg aggatagata
     1381 ttccgacatc agttacggca ttaaacttac cggcggtaat atcgggtata gcgaggaaat
     1441 ctatactttt ccatattttt gcaccttttt attgaagagg tatctatcgg attaactaat
     1501 cccttttgat gttgaaggca atctttaaaa cgggtatact gttagcagta aaatactgaa
     1561 gtggaggttt tcgtatggat tacaaaagaa cggatgagat aatcaaaaga acaaaggata
     1621 tgtatgagtc gggaaaaggt gcccttatac aggttaagga gatattcagc ggaattgatg
     1681 tgccttttcg tccgttggac agtttcggat tacctaagaa tatggaaact tatctggatt
     1741 atatgattga aagacagcgt atcttctgga atatacgcaa tcaaatatcc gatgacataa
     1801 tacaatccat tacggtacgc tacggtatag cggaacattc agcttttatg ggcggagacg
     1861 tcaccttttc cgaaaccact tcatggcatc atcaacatgt gaaagattat tccgattaca
     1921 ctcttgcgaa ggttgatgag tcaaatatat ggaggaatct tgttatagaa ggaatgaaat
     1981 atatgaagag caaggtgaaa gatgagttct ttataagatt cagaggtgcg gacggtccgt
     2041 tggatgtggt caatgcatta cgcggaaacg atatatttta cgatttttat gatgaagaag
     2101 aaggtttgtt ggagcttacg tcaaaatgca ccgatgcaat aatata
//
LOCUS       DAOPWC010000286         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_56249_length_2116_cov_2.901504, whole genome shotgun sequence.
ACCESSION   DAOPWC010000286 DAOPWC010000000
VERSION     DAOPWC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56249_length_2116_cov_2.901504"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PKJ65_06220"
     CDS             <1..1076
                     /locus_tag="PKJ65_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNZ41465.1"
                     /translation="AGYIETKYLDIKVTSKQPVVVRYEQKKLYLPKYVVLSWHYVHSS
                     SVTYVPYYADVNVLSPTFFTVIDEKGNIASSASVKYMENARKRGYTVWPLINNVFAKK
                     GQISEVLADSEARRHVIDQILTYAYIYKFDGINIDFENLYLSDKDNLTQFMRELVPLA
                     HQMNLAVSIDVGVPGGSEAYSRCYDHEELGKVADYVMVMTYDQYWSAHETGGSQAQLS
                     WVEENVGKTLKLIEPDKLVLGIPAYTRLWKTDSEGKVTLDKTLTADQVVKLVEEKQLT
                     PVWEEERDGYISGQYYIEYIEDGELYRAWIEDDASAREKAALAAKYNLRGVCIWMMSQ
                     TNKTVWEAILEGFNADVNQVISD"
     gene            1155..1646
                     /locus_tag="PKJ65_06225"
     CDS             1155..1646
                     /locus_tag="PKJ65_06225"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNZ41466.1"
                     /translation="MKVRVNYEDIFERLNGQLPEGVFLNTVSEGKYNTMTIGWALMGI
                     CWGQPIVCVAVRFSRYTYELLEQSGKFTLSIPESGVMQKQLALCGSKSGRDVDKFELC
                     GFKKSNIIDKCDLHIECEVVQKTAMNKDFIEKSIKSRYYRTDDYHMFYFGKITDCYYT
                     KEG"
     gene            1650..>2116
                     /locus_tag="PKJ65_06230"
     CDS             1650..>2116
                     /locus_tag="PKJ65_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNZ41467.1"
                     /translation="MKNVCVIFGGASTEHEVSRVSASIVMKNLDKKKYSITVMEMTKN
                     REYLLYEYREGEAFLEEIVKVDNNLPRKRIIPDISMFERMGVDVVFPVTHGTIGEDGK
                     LQGFLELCDIPYVGCDVLSSAVCMDKVLCKDILLKNGIAVADYLWFTCDEIGK"
BASE COUNT          681 a          331 c          529 g          575 t
ORIGIN      
        1 ttgcgggata catagaaaca aaatacctgg atattaaagt tacctctaaa cagccggttg
       61 tagtcaggta tgagcaaaaa aagctgtatc tgccgaagta tgtggtgctt tcctggcatt
      121 atgtacattc cagttcggta acatatgttc cgtattatgc cgatgtcaat gtcctgtctc
      181 ccacattttt tacggttata gatgaaaaag gcaatattgc cagcagtgca tccgtgaagt
      241 atatggaaaa tgcaagaaaa agagggtata ccgtctggcc gttgattaat aatgtttttg
      301 caaagaaagg gcagataagt gaagtattgg ctgacagtga ggcaaggcgt catgtgatag
      361 accagatact gacttatgcg tatatatata aattcgacgg gataaatata gattttgaaa
      421 acctgtattt gtccgacaag gataatctca cacagtttat gcgggagttg gttcctttgg
      481 cacatcagat gaatctggcc gtttccatag acgtgggagt tccgggagga tcggaggctt
      541 attcacggtg ttatgatcac gaagaactgg gaaaagtagc cgattatgtg atggtaatga
      601 catatgacca atactggagt gcacatgaga ccggaggctc gcaggctcag ctttcctggg
      661 tggaagagaa tgtaggcaag actttaaaac tgattgagcc ggataaactg gttttaggta
      721 tacccgcata taccagattg tggaaaaccg acagtgaagg aaaagtgact ttggataaga
      781 cactgacagc cgatcaggta gtaaaacttg ttgaggaaaa gcagttaact ccggtatggg
      841 aagaagagag ggacggatat ataagcggac agtactatat tgaatatata gaagacgggg
      901 agctttacag ggcatggata gaagatgatg ccagtgcaag agaaaaagcc gcacttgccg
      961 ccaaatacaa cttaagaggt gtatgtatct ggatgatgag tcagaccaac aaaacggtat
     1021 gggaagccat tctggaaggt ttcaatgcgg atgttaatca ggtaatatct gattaaaccg
     1081 atttttcgtt ttcttatgtg ctttaatgtg gatatggtat ataattaatt aaaacgtcgg
     1141 aaaggaaaaa ctgaatgaaa gttagagtga actatgaaga catctttgaa agattaaacg
     1201 gacaactgcc ggaaggtgtt tttctcaata ctgtttctga aggcaaatac aatacaatga
     1261 ccataggctg ggcattgatg ggaatatgct ggggacaacc tatcgtatgt gttgcggtca
     1321 gattttccag atacacatat gaacttttgg agcagtcggg taaattcaca ttaagcattc
     1381 ccgaaagcgg tgtaatgcaa aagcaacttg cactctgcgg ctcaaaatcc gggcgcgatg
     1441 tggacaagtt tgaattatgc ggatttaaga aaagcaatat tatagataag tgcgatcttc
     1501 acattgaatg tgaagtggtg cagaaaaccg caatgaacaa agactttatt gagaaatcta
     1561 taaaaagcag atattacaga acagatgatt atcatatgtt ctatttcgga aagataaccg
     1621 actgctatta tactaaagag ggttgaaata tgaaaaatgt atgtgtaatt ttcggaggtg
     1681 cttccacgga acatgaggtc tcccgcgtat ccgcctcaat cgttatgaaa aacctggata
     1741 agaaaaagta ttcgattacc gtaatggaaa tgactaaaaa cagggaatat ttactgtatg
     1801 agtataggga aggtgaagct ttcttagaag aaatagtcaa ggtggataac aatcttccca
     1861 ggaaacgcat tattccggat atttccatgt ttgaaaggat gggtgtggat gttgtttttc
     1921 ccgttactca cggaacaata ggggaagacg ggaaattgca gggtttcctg gagttatgtg
     1981 atattcccta tgtaggctgc gatgttctgt cctcggctgt gtgcatggat aaagtgcttt
     2041 gcaaagatat attgttgaaa aacggtatag ccgttgcgga ctatttatgg tttacctgtg
     2101 atgaaatagg taaaga
//
LOCUS       DAOPWC010000287         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_56898_length_2099_cov_3.381605, whole genome shotgun sequence.
ACCESSION   DAOPWC010000287 DAOPWC010000000
VERSION     DAOPWC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56898_length_2099_cov_3.381605"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKJ65_06235"
     CDS             <1..561
                     /locus_tag="PKJ65_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ41468.1"
                     /translation="DLVKSATFKKEQQGITSLLMVLTKWSSVRNDVVYCLQKTDETKL
                     SEPIGGYIREMCIRLEKGMSVSEALSACQENALSEELRYVLINIRYAYEKGGSLYRIF
                     KSLENQFFKIDEENFKRKINTLSDKYAVYLSIVMVMATFYMVVLNKGQSGQYYLKTET
                     GMLLLGVFALLFFAGMLIITKVIKRY"
     gene            564..1355
                     /locus_tag="PKJ65_06240"
     CDS             564..1355
                     /locus_tag="PKJ65_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41469.1"
                     /translation="MSEWVRSIIYLGNFGVGILFASHIACMKLGSRDKKTTFGRIEDV
                     ISGFLCKISGKMGIPEEKTKDFTAITIFFTILCLAVAVTAGIIKGFIILIPCVICIYL
                     MKKAWEKNIGEMFQKNAYRLYRYLVSQITAGVKPKDVIINMYKITEDKSLKKILTRAS
                     AAYSITLDGKTYSDVIKSSIKTEDADRFCMILEEDLLYTDNEEFLEKMEEMMFNRHFS
                     YHQKKTDSIKRGCFAAVCVFMLIVIIMVALPLITELVQGLKYIFS"
     gene            1368..1559
                     /locus_tag="PKJ65_06245"
     CDS             1368..1559
                     /locus_tag="PKJ65_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41470.1"
                     /translation="MIVNQQSKRGFGISEILGIATVLIIAAAVVIPGLRDLAKTILES
                     TKTWVQGKMGTIFNLAPGN"
     gene            1570..1944
                     /locus_tag="PKJ65_06250"
     CDS             1570..1944
                     /locus_tag="PKJ65_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41471.1"
                     /translation="MAERIIVGIVSLLIILCVFVYCTDVLTVISKNMEFYDICRGYMH
                     IAEQNSGLSYEEKTRLKERLIAGGFDNVVITAPEAAVYGSMFNLNVKASYSINMITDI
                     FTRNIQEYVMNFDQNIAARKIR"
     gene            1946..2077
                     /locus_tag="PKJ65_06255"
     CDS             1946..2077
                     /locus_tag="PKJ65_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41472.1"
                     /translation="MDIKKKGSGISEVLISVFVLIYIMLPLFCSVVNKAMFYHSVQE"
BASE COUNT          683 a          333 c          493 g          590 t
ORIGIN      
        1 gatcttgtaa agagtgccac atttaaaaaa gaacagcaag gtataacgtc gttgttgatg
       61 gtactgacca aatggagttc ggtacgaaac gatgtggtgt attgtctgca aaagaccgat
      121 gaaacaaagc tgtccgaacc tatcgggggg tatataaggg aaatgtgcat caggctggaa
      181 aagggtatga gtgtttccga agcgctgtct gcatgccaag agaatgcttt aagtgaagaa
      241 ctccgttatg tccttataaa catccggtat gcatatgaga agggcggaag tctctacagg
      301 attttcaaaa gcctggaaaa tcagtttttt aagattgatg aagagaattt taaaagaaaa
      361 attaatacct tatcggataa atatgccgta tacctttcaa ttgttatggt tatggctacc
      421 ttctatatgg tagtactcaa caaaggacag tcgggacaat attatcttaa aaccgaaacg
      481 ggcatgttgc ttttgggtgt atttgcactt ctgttctttg caggaatgct tatcatcacc
      541 aaagtgatta agaggtattg agtatgagtg aatgggtaag aagcatcata tatttgggta
      601 atttcggagt gggtatactg tttgcttcac acatagcatg catgaagttg ggcagtcgtg
      661 ataaaaagac aacattcgga agaattgaag atgtgataag cggctttttg tgtaagattt
      721 ccgggaaaat gggaataccg gaggaaaaaa ccaaagactt taccgccatc acaatatttt
      781 tcacaattct gtgtttggca gtagccgtaa cagcgggaat aattaaagga ttcatcatat
      841 taattccctg tgttatttgc atttacctta tgaagaaagc ctgggaaaag aatataggcg
      901 aaatgtttca gaaaaacgca tacaggcttt atcgatatct tgtgtctcag ataacggcgg
      961 gagtaaagcc gaaggatgtg attataaaca tgtataagat tacggaagat aaaagtctta
     1021 aaaagattct taccagggct tcggccgcat attccattac tttggacggt aaaacctatt
     1081 cggatgtaat caaatcttcc ataaagaccg aagatgccga caggttctgc atgattctgg
     1141 aagaggacct tctatatacc gataatgagg agtttttaga aaaaatggag gaaatgatgt
     1201 tcaacagaca tttttcctat catcagaaaa agaccgacag cattaaaaga ggctgctttg
     1261 cggcagtgtg cgtattcatg cttattgtaa taatcatggt tgctctgccg cttataaccg
     1321 agcttgtgca ggggctcaaa tatatatttt cataggagga gaagaaaatg atagtaaatc
     1381 aacaaagtaa aagaggattc ggcatttccg aaatattagg aatagcgact gtattgatta
     1441 ttgcggctgc ggttgtaata ccgggactca gagatcttgc aaaaacaatt ttggaatcca
     1501 caaaaacatg ggtgcagggc aagatgggaa ccatcttcaa tttagctccc ggaaactgac
     1561 gtaaagcata tggctgaaag gataatagtc ggtatagtct ccttactcat aatactgtgc
     1621 gtgtttgtat actgcaccga tgttcttacg gttatatcaa aaaacatgga gttttacgat
     1681 atatgcagag gatatatgca catagccgag cagaattccg gattgagtta tgaagaaaag
     1741 acccgtttga aggaacgtct cattgcaggg ggctttgata atgttgttat cacagcccct
     1801 gaagcagccg tatacggaag catgtttaac ctgaatgtta aagcatcata cagtataaat
     1861 atgataacgg acatatttac caggaatata caggagtatg tgatgaattt tgaccagaat
     1921 atagcggcaa gaaagatcag gtgacatgga tataaagaag aaaggttcgg gaatatccga
     1981 agtacttata agtgtttttg ttcttatcta cataatgctt ccgcttttct gttcggtagt
     2041 caataaggcg atgttttatc acagtgtgca agaatagagc aggctgtgga gatggcggt
//
LOCUS       DAOPWC010000288         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_57176_length_2092_cov_2.727050, whole genome shotgun sequence.
ACCESSION   DAOPWC010000288 DAOPWC010000000
VERSION     DAOPWC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57176_length_2092_cov_2.727050"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..501)
                     /locus_tag="PKJ65_06260"
     CDS             complement(79..501)
                     /locus_tag="PKJ65_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNZ41473.1"
                     /translation="MIQYPAKIKYSKSDNSYLVEFPDLPGCHTFGDTLGEAQEMAAEA
                     LSGYLESIDLRKMDIPKPSKLTGKDIYYIAPEAKTAFAIWMKLKRIEKGLTQQKAAEL
                     LEINFQSYQKFENPKKANPTLTTLKKIERVFHEKIVLV"
     gene            complement(498..677)
                     /locus_tag="PKJ65_06265"
     CDS             complement(498..677)
                     /locus_tag="PKJ65_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNZ41474.1"
                     /translation="MTGKELVKLLKKDGWTLDRIKGSHHIMIKGTMTLSVPVHGNRDL
                     PAGILHRLMKDGGLQ"
     gene            complement(729..1595)
                     /locus_tag="PKJ65_06270"
     CDS             complement(729..1595)
                     /locus_tag="PKJ65_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ41475.1"
                     /translation="MKNHIIPYLGNIYIFDIKYKTLEQYRNDKFKEGYTGSFIVKHLH
                     TINTILKEACKDELIQAPPLLPECSTAVLNPRGCLTVEEVKTLFTKGIWKDYRAFAAS
                     LLSASTGLRLGEIQGLVMADLHLPESYLVCRRSYDYRNRCLNETTKNGRSRNIILSSH
                     VVYALQRVISTNPHPLNDESPVFWSDFIPDNPTERNTLIKGLYRALASIGINDNQRRQ
                     RNISFHSHRHFLNSLLINAKIPLQKVQSITGHLTAEMSQHYYKLDDMSDILQITESIV
                     TTSNQNIQEMMN"
BASE COUNT          532 a          418 c          422 g          720 t
ORIGIN      
        1 acgtaggaag cgtccttgtc ggtacggctc atgacttccg cacctatccc gtgatgtgta
       61 ttattaaaat aataccatct atactaaaac gatcttttcg tgaaatactc tctctatctt
      121 cttaagtgtt gtcaatgtcg gatttgcctt tttgggattc tcaaattttt ggtagctctg
      181 aaaatttatc tcaagcagtt cagccgcttt ttgttgagtg agtccctttt cgatccgttt
      241 cagcttcatc catatagcaa aggccgtttt cgcttcaggg gctatgtaat atatgtcctt
      301 tccggtcagt ttagaaggtt tcggaatatc catctttcta aggtctatcg attccagata
      361 ccctgaaagg gcctctgctg ccatctcttg agcttctcca agtgtatccc cgaaggtgtg
      421 gcatcccgga aggtctggaa attctacaag ataggaattg tcgcttttgg aatattttat
      481 ttttgcgggg tattgaatca ttgtaagcct ccatccttca taagtctgtg taaaatacca
      541 gcgggtaaat ctctgttccc gtgaacaggc actgaaaggg tcatcgtgcc tttaatcatt
      601 atatgatgtg atccttttat cctgtccagt gtccacccgt cctttttaag cagtttaaca
      661 agttctttcc ctgtcatagt ttaatatacc gcctttagta tgtattgtca attctttttt
      721 atatgtcttc aattcatcat ttcctgtata ttctgattgg aagttgtgac aattgattcc
      781 gttatctgaa gtatgtcgct catgtcgtcg agcttatagt aatgctggga catttcggca
      841 gtgaggtgtc ccgtaatact ctgtactttc tgcaagggga ttttggcatt tataagtaac
      901 gagtttaaga aatgtctatg actgtggaac gagatatttc tttgtcgcct ctgattatca
      961 tttattccaa tagacgcaag agcccgataa agtcctttta tcagggtatt tctttccgta
     1021 ggattatcag gtatgaaatc cgaccaaaag acagggcttt catcattaag agggtgcgga
     1081 ttcgtagaga tcaccctttg caatgcatat acaacgtggg atgaaagaat tatgtttcgg
     1141 cttcgtccat tttttgtcgt ttcatttaag catctgtttc tataatcata agaacgcctg
     1201 caaaccagat aactttcagg caggtgtaaa tcagccataa caagaccctg aatctctcca
     1261 agccttagac ctgtagaggc tgaaagaagc gatgctgcaa aagctctgta atccttccag
     1321 atgccttttg tgaaaagtgt cttaacttct tctacggtta aacagccacg gggatttaat
     1381 accgctgttg aacattccgg caacaaaggc ggtgcctgaa ttagttcgtc tttgcaggct
     1441 tctttcagta tcgtattgat tgtatgcagg tgcttaacga taaaagaccc tgtatatcct
     1501 tccttgaatt tatcatttct gtattgttca agagtcttgt atttgatgtc gaaaatatat
     1561 atattaccta aatatgggat gatatgattt ttcattatat cccggcgctc tttgagatga
     1621 tgaagtccta ttcgtttacc tgaattatgc ttgttggtcg cataaatacc tgtatcatca
     1681 aaaaaacctc ttgcatattc agcaaatgta atccgtttat cgatgaaaaa acgattattc
     1741 ttcaggtatt cgatagccca ggactcggcg cgtgcttttg aaatttgtcc tgaagatttc
     1801 gatgttgacc tgcttccatc cggaagacga aatctaacat aataaacttg atttcttttt
     1861 aataatgtga aaggttttga gtgtgacatt ctaatacctc tgttttgtgc catatctgtt
     1921 ggtcacagtt tggtcacatt ttgggattta gaagcacacc cgttttcggg aaggctttat
     1981 aacccctgat taaatcagag gttatgagcg ggtgacgaga ctcgaactcg cgacgttcag
     2041 cttgggaaga agcggaaccc tttttccgta tgtgcttcta tatgcttgtt ta
//
LOCUS       DAOPWC010000289         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_57201_length_2091_cov_3.368861, whole genome shotgun sequence.
ACCESSION   DAOPWC010000289 DAOPWC010000000
VERSION     DAOPWC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57201_length_2091_cov_3.368861"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKJ65_06275"
     CDS             <1..507
                     /locus_tag="PKJ65_06275"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HNZ41476.1"
                     /translation="ISIGFSNAGLLNMEALVFLPEVRQMCNSDSCRSFNKNWMCPPAI
                     GTLEQSADKVKEYSFGMIVQTTAFMDDEFDYETIRETQKKHNNSFLELATILRKEYPS
                     VLPMGAGACSICSQCTYPDKPCRFPDKAIGSMESYGLWVSKVCEKSSIPYYYGKNTLT
                     FTSCFLLK"
     gene            693..1337
                     /locus_tag="PKJ65_06280"
     CDS             693..1337
                     /locus_tag="PKJ65_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ41477.1"
                     /translation="MISEIELLENNKIYIAGSIASSPVFNHQIYGESFYNIVVEVQRL
                     SKQTDMVPVLISGHLLDIDLLKPDTLIEINGQVRSYNNIDKNEKKRLVVNVFAREARI
                     LQTLEGIESYNDAFFDSFICKEPNFRITPFGREICDLLIAVNRAYKKSDYIPCICWGR
                     NARYCENMKVGTRLKIWGRFQSREYEKKDHYGNIVTKTAYEVSVIKLEKVEENK"
     gene            1391..>2091
                     /locus_tag="PKJ65_06285"
     CDS             1391..>2091
                     /locus_tag="PKJ65_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019912914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ41478.1"
                     /translation="MLRKARKADRLLKSGKGAGSDFLGWLDLPKRIKKEDIKLINKAA
                     AKIQKQSDVLIVIGIGGSYLGARAAIEALTDVRNNKGTEVIFVGNNISGRYVESVLEY
                     IKDKNYSLNVISKSGTTVEPAIAFRIFKEALYEKYKEKAKDRIYITTDPVKGALRSIV
                     KKEGYTSFEIPADIGGRYSILTPCGLLPIAVAGIDIEQMISGAKDALKNCTDMSVTYA
                     AARNLMYKSKKTVEI"
BASE COUNT          771 a          318 c          449 g          553 t
ORIGIN      
        1 atatccatag gattttccaa cgcgggactt ctgaatatgg aagcattggt tttcctgccg
       61 gaagttcgtc agatgtgcaa ttccgactcc tgcagaagtt ttaataagaa ctggatgtgt
      121 ccgccggcaa taggaacgct ggaacaatca gccgataaag taaaagaata cagcttcggt
      181 atgatagttc agactaccgc atttatggat gacgagtttg attatgaaac tatacgggaa
      241 acgcagaaaa aacataacaa ttcattcttg gaattagcaa caatacttag aaaagaatat
      301 ccttcggttc ttcctatggg agcgggtgca tgttcaatat gcagtcaatg cacttacccc
      361 gataagccgt gcagatttcc cgataaagcc ataggctcca tggaatcgta cgggttgtgg
      421 gtaagcaagg tatgtgagaa gtcatcaatt ccttactatt acggaaaaaa tacactgaca
      481 ttcacaagct gctttttgct taaatgatat ataaaacggt tttgaatact taaaaacatt
      541 aacaggaaac atatcaaaaa catataatta acatgaatac cgtactaatt gaaaaatttt
      601 cctttatttt tgtataaagt gataatataa taagggaaaa gtcataagac ttacataaga
      661 atgttaatta caaaagaaag tctggtatgc aaatgataag cgaaatcgaa cttttggaga
      721 ataacaagat atatattgcg ggaagtattg catcttcgcc cgtattcaat caccagatat
      781 atggtgagtc tttttataac atagtcgtgg aggttcagag actgagtaaa caaaccgata
      841 tggtaccggt attaatatcc ggtcatcttc tggatattga tttattaaag cccgatacat
      901 tgattgagat taacggacag gttcgttcat ataacaatat tgataaaaac gaaaaaaaga
      961 gattggtagt gaatgttttc gcaagagaag ccagaatctt acagactctg gaggggatag
     1021 aaagttataa tgacgccttt tttgattcat ttatctgtaa ggaacccaat ttccggatta
     1081 ctccgttcgg cagagaaata tgcgacctgc ttatagctgt aaacagggcg tataaaaaaa
     1141 gcgactatat accctgtata tgctggggaa gaaatgccag atattgcgag aacatgaaag
     1201 taggtacacg cttaaaaata tggggtagat ttcagagcag agaatatgaa aaaaaagatc
     1261 actacggaaa tatagtaact aaaacggcat atgaagtgtc ggtaataaaa ctggaaaaag
     1321 tagaggagaa taaatgaaaa taattataga cgattgtata tgtttttcag aacagaaaaa
     1381 atttgaaagt atgttgagaa aggcaagaaa agcggacaga ttattaaaaa gcggaaaagg
     1441 tgccggctcg gattttttag gctggctgga tttacctaaa agaataaaaa aagaagacat
     1501 aaaacttatt aataaagccg ctgcaaagat tcagaaacag tcggatgtac ttattgtaat
     1561 aggtataggc ggctcttatt taggtgcaag agcggcaatt gaagctctta cagatgtcag
     1621 aaataacaaa ggtacggaag taatatttgt aggaaacaat ataagcggca gatatgtcga
     1681 atccgttctg gaatacatca aggataaaaa ctactctttg aatgtaataa gtaaatcggg
     1741 tactacagtg gaacctgcaa tagctttcag aatatttaaa gaagctttat atgaaaagta
     1801 taaagaaaag gcaaaagaca gaatctatat cacaaccgac cctgttaaag gtgcattgcg
     1861 gtcgatagtg aaaaaagagg gatatacaag ttttgagata cctgccgata taggtggaag
     1921 atacagtata ctcacaccgt gcggattgct tcccatagcg gtagcaggca ttgatataga
     1981 acagatgatt tcaggtgcta aagatgcttt aaagaattgt acggatatgt cggtaacata
     2041 tgctgcagcc agaaacctga tgtacaagag caaaaagacc gtagagatta t
//
LOCUS       DAOPWC010000290         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_57565_length_2082_cov_3.170696, whole genome shotgun sequence.
ACCESSION   DAOPWC010000290 DAOPWC010000000
VERSION     DAOPWC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57565_length_2082_cov_3.170696"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKJ65_06290"
     CDS             complement(<1..485)
                     /locus_tag="PKJ65_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ41479.1"
                     /translation="MYSIGIDLGGTAIKAGIVDKSGNIIVKDKVPTKSERGYEAVLRD
                     MAKLCKKLIEKAGMSESDIEKIGVGCPGTPDKVNGILIYNNNLGMRNVNIRKELQKYF
                     ALPVYIDNDANVAALAESVCGAVKGTYCSVTITLGTGIGSGVVIGNKVYSGFNYAAAE
                     IG"
     gene            complement(496..1293)
                     /locus_tag="PKJ65_06295"
     CDS             complement(496..1293)
                     /locus_tag="PKJ65_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ41480.1"
                     /translation="MQGITIGQYIPGDSIIHKADPRLKIIMTFLTMILLLIIDSFYPL
                     LLSAVFVVTAYLVSGISLRYAIKGLKPVIYIVIFTMILNIFFATGTPLFTLGFITVTK
                     EGLVTSALVSARILLLISAGSLMTYTTTPLVFTVGIEKLMYPLKYLKVPIAEIAMMIS
                     IALRFIPTLMEETEKIIKAQAARGADIGTGNIVARTRSFIPVLVPLFISSFKRADDLA
                     TAMDARCYNGSEGRTSISELKYGKIDLAIFMVYILFVAAALVLQYVL"
     gene            complement(1351..1823)
                     /locus_tag="PKJ65_06300"
                     /pseudo
     CDS             complement(1351..1823)
                     /locus_tag="PKJ65_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(1825..>2082)
                     /locus_tag="PKJ65_06305"
     CDS             complement(1825..>2082)
                     /locus_tag="PKJ65_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41481.1"
                     /translation="DALVGKNIFDKVIEQGTGKLKYIMSDISRKWLTLDCLVDKITIQ
                     KLPQGDIINVSITKSGMMTVTQNAEGLRFKTITINEFYEEK"
BASE COUNT          544 a          522 c          358 g          658 t
ORIGIN      
        1 cctatttccg ctgcagcata attgaatccg ctgtaaacct tattgccgat aaccactccg
       61 ctgcctattc cggtacctaa tgttatggta accgaacagt atgtaccttt gaccgctccg
      121 cagacacttt ccgcaagcgc cgcaacattg gcatcattgt caatgtatac gggaagagca
      181 aaatacttct gcaattcttt ccttatattc acatttctca ttccgagatt attgttatag
      241 ataagtatgc cgtttacctt gtcgggagta cccgggcatc ccacccctat tttttctatg
      301 tcgctttcac tcatcccggc tttttcgata agctttttgc agagtttggc catatctctc
      361 aatacggctt cgtagcctct ttcgcttttt gtcggcacct tgtccttaac aattatatta
      421 ccgcttttgt ctactatacc cgccttgatt gcggttcctc cgaggtcaat tccgatactg
      481 tacatgtctt ccctcctata acacatattg cagcaccaaa gcggctgcaa caaacaaaat
      541 atataccata aatatggcaa ggtcaatctt tccgtacttc aattcgctta tacttgtacg
      601 cccctccgaa ccgttatagc agcgggcatc catggctgtc gccagatcgt ccgctctctt
      661 gaaagaggat atgaaaaggg gcaccaaaac cggtatgaaa ctcctggttc tcgcaactat
      721 attccccgta cctatatccg ctcctctggc tgcctgagcc ttaattatct tctcggtctc
      781 ttccatgaga gtaggtatga acctcaatgc aatggatatc atcatggcta tttccgctat
      841 gggaacttta agatatttca aaggatacat cagcttctcg atacccactg taaacaccaa
      901 aggtgtcgta gtatatgtca taagcgaccc tgcggaaatc aacaacaata ttcttgcact
      961 taccaaagcc gatgtcacaa gcccttcttt agtaacggtg ataaatccga gagtaaataa
     1021 cggagtaccg gtcgcaaaga aaatattcag tatcatggta aatatgacaa tatatatgac
     1081 aggtttgagt ccttttattg cgtatcgcaa actgattccc gaaacgagat aagcagttac
     1141 gacaaacacc gcacttaaaa gcaacggata aaaagagtca attatgagta agagaatcat
     1201 ggtaagaaag gtcatgatta tcttaaggcg cggatcggcc ttatgtataa ttgaatctcc
     1261 cggtatgtat tgtcctatag ttattccctg cacttaaaaa gtctcctgtc caatccgata
     1321 aattatatca caattgtttc catatacaac ctaaacacag ccggtaccgt tctttcgttc
     1381 cttacgctta taatacctct ttttacccat ttcccagtcg catcgctctt cttcggtttc
     1441 caatattaaa ccgggaactt ccaccggtct ttcattctca tccaatgcca ccattacgac
     1501 atatgctaca tttatgagtt tcttttctcc gttcaactcc tccacatacg acttgacaca
     1561 tacttccatg gaggtttttc cgacatatgt cagcttagcg gtgagaagca ccgtatcgtt
     1621 ggcataagcc ggtcctttaa attccagcga atccaccgct accgtagtaa cattgcggtt
     1681 ggaatgtctt cttgcggcta ttgcggcggt tatgtctatc cattccataa tacgtcctcc
     1741 gaacagtctg tcattgccgt taatgcggac tgggttaata tctgtgcctg ttcggtatat
     1801 gaatctctaa catacttttt catcctattt ctcctcgtaa aattcattaa ttgttatggt
     1861 cttaaaccgt aatccttccg cattttgtgt tacggtcatc attcccgact ttgttatact
     1921 cacattgatt atatctccct gggggagttt ctgaatggtt attttatcca caaggcagtc
     1981 taaagtaagc cactttcttg aaatatcgct cataatatat ttcagttttc ccgtaccttg
     2041 ttcaatcact ttatcgaata tatttttccc gaccaatgca tc
//
LOCUS       DAOPWC010000291         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_58154_length_2069_cov_2.714995, whole genome shotgun sequence.
ACCESSION   DAOPWC010000291 DAOPWC010000000
VERSION     DAOPWC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58154_length_2069_cov_2.714995"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..1653)
                     /gene="glmM"
                     /locus_tag="PKJ65_06310"
     CDS             complement(307..1653)
                     /gene="glmM"
                     /locus_tag="PKJ65_06310"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNZ41482.1"
                     /translation="MGKIFGTDGARGIANKELTVSLAFDLGKAGAYVLAGENRHKPKI
                     LIGADTRISSDMLMSATEAGLCAMGAEVIRAGVITTPAVAYLTKFYNCDAGIVISASH
                     NSYEYNGIKFFNRDGFKLPDETENHIESIITDKVEISVKSHDEIGRIHKDCECVEAYC
                     DFLIEKCNTGLSKLNILLDCANGAASAIAPKVFERTGCKVAHIHSSPDGLNINRECGS
                     THINSLAEHVKEGGFDVGFAYDGDADRLIAVSERGEVIDGDVLMSVLALYLRGKGLLY
                     KDTLVATEMSNMGITVFGKNNSIEIIRTKVGDRYVTEEMRRGGYAVGGEQSGHIVLLP
                     YTTTGDGILASLKIAEILAENNIKASSFFEIMKPLPQVLVNVRVDNEMKGKLKDDMEI
                     QSLSEKVSTELGGAGRVMVRASGTEPLIRVMIEGEDYAFIENRANMIADMIRLKLG"
     gene            complement(1725..>2069)
                     /locus_tag="PKJ65_06315"
     CDS             complement(1725..>2069)
                     /locus_tag="PKJ65_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41483.1"
                     /translation="ILSLGNNVVLSESPALNIYVETIATYSIDSNRILLTNIDTNLIY
                     SIQNETFNMRLVGLSADISDTDISEMKFYVNVQDLREGVHMVDVIVKDVNLPENVRFY
                     EDMQVAVEITAP"
BASE COUNT          550 a          505 c          352 g          662 t
ORIGIN      
        1 ctacgaggag gtaaaattat gaaattcaaa acaaagaatc actacaagca tgatgctgag
       61 tatgctcttt gttttgaatt tcataatttt acctcctcgt agtaaataac tctttataaa
      121 taaagtttta gtcataatat catgcacatg aaaaagtgtc aacatatttt ttcacattga
      181 gttcacattc cggacacaat ttgcataaaa atgactaact aatgctgttt tttaggtttt
      241 ctaagtaaat tcatggtttt aaacttatga aaaatgaata aatcggtttc aaaatattca
      301 tatttatcag ccgagtttta atcttatcat atcggctatc atattggccc ggttttctat
      361 aaaagcgtaa tcctcgccct ctatcattac tcttatcaga ggttcggttc cggaagccct
      421 taccatcacc cttccggcac cgcccaattc cgtggaaact ttctctgaaa gtgactgaat
      481 ttccatatca tccttcagct tgcccttcat ttcattatcc actcgcacat tgacaagcac
      541 ctgaggaaga ggtttcatta tttcaaagaa gctgcttgct tttatgttat tctccgcaag
      601 tatctcggca attttcaacg atgctaaaat accgtcaccc gttgtggtgt acggtaaaag
      661 cacaatatgt cccgactgtt ctcctccgac tgcatatccg cctcttcgca tctcttccgt
      721 cacatatctg tctccgactt ttgtccttat aatttctatg gagttgttct tgccgaaaac
      781 ggtaataccc atattgctca tttcggtggc caccagggtg tctttgtaca gcagtccttt
      841 gcctcttaag tacagagcaa gcaccgacat aagcacatca ccgtcaataa cttctcccct
      901 ttccgatacc gcaataagcc ggtcggcatc tccgtcataa gcaaaaccta catcaaaacc
      961 gccttcttta acatgctccg ccagactgtt gatatgagtg gatccgcatt ctctgtttat
     1021 attcagaccg tcgggtgagg agtgaatgtg tgcaactttg catccggtcc tttcaaatac
     1081 tttgggagct atcgcacttg cggcaccgtt tgcacaatcc aaaaggatat tcagttttga
     1141 aagaccggtg ttgcattttt ctataagaaa atcacaatac gcctcgacac attcacaatc
     1201 cttatgtatt ctgcctatct catcatgtga ctttacactt atctcgactt tatcggtaat
     1261 aatactctct atatgatttt ccgtctcatc gggcagtttg aatccgtccc tgttgaagaa
     1321 ttttatcccg ttatactcgt aggaattgtg tgaggcggat atgacaatac ccgcatcgca
     1381 gttgtaaaac tttgtaagat atgctaccgc aggggtggta atcactcccg cccttataac
     1441 ctcggctccc atcgcgcata agcccgcttc ggtagcggac ataagcatat ccgacgatat
     1501 tctggtatcg gctcctatca gtatcttggg tttatgacgg ttttcccccg ccagaacata
     1561 agcgcctgct tttcccaaat caaaagcaag gcttacagtc aactctttat tggctatacc
     1621 ccttgctccg tcagtaccga atatttttcc cattgaatcc tccacaactt aatttgtgct
     1681 ttttgcttta gctctttata tactcaattt ctgtcatccg ctcattacgg tgcggttatc
     1741 tctaccgcaa cctgcatatc ctcataaaac cgcacattct ccggaagatt gacgtctttt
     1801 actattacgt ccaccatatg tacaccttct ctaagatcct gtacatttac ataaaacttc
     1861 atttcggaaa tatctgtatc ggagatatcg gcacttaaac ccacaagcct catgttaaaa
     1921 gtctcattct gtatggaata gataagattt gtgtctatat tggtcagcaa tatcctgttg
     1981 ctgtcaatcg aataggtcgc tatggtctca acatatatgt taagggcagg cgactcggac
     2041 agcaccacat tgtttcccag tgacagtat
//
LOCUS       DAOPWC010000292         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_58983_length_2050_cov_2.533333, whole genome shotgun sequence.
ACCESSION   DAOPWC010000292 DAOPWC010000000
VERSION     DAOPWC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58983_length_2050_cov_2.533333"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="serS"
                     /locus_tag="PKJ65_06320"
     CDS             <1..922
                     /gene="serS"
                     /locus_tag="PKJ65_06320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ41484.1"
                     /translation="ATQNQPVRYHGRKPEFDFAIKDHVALCEALGIIDYKRGAKLSGS
                     GHWMYRGDGALLEWALLNYFITEHIKDGYEFILPPHILNSECGYTAGQFPKFGDDVFK
                     LTENDQFLLPTAETALINLHRNEVLREEELPKKYFAYTPCYRKEAGSYRAEERGMIRG
                     HQFNKVEMFQYTVPEKSEEALMELVAKAETLMNGLGLHFRLSKLAAADCSFAMGKTYD
                     VEVWIPSMGIYKEVSSASNAYDFQAIRGAMRFKRTATGKNEYLHTLNASGLATSRVFP
                     AIIEQNQHKDGSVTVPEVLRKWVGKDKLTV"
     gene            919..>2050
                     /gene="lysA"
                     /locus_tag="PKJ65_06325"
     CDS             919..>2050
                     /gene="lysA"
                     /locus_tag="PKJ65_06325"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNZ41485.1"
                     /translation="MIEYFSEKTDFYKHHKPSELLSAYGSPLYVYNEDILRERARQIK
                     NFVNMPNFTVSYSVKANCNLHILKILKEEGLHADAMSEGEIFVTEQAGYEKDRIFFVC
                     NNVSAREMKYAVDRGIMVSVDSLSQLELYGRNFPKTKVAVRINPGIGAGHHKKVVTAG
                     EATKFGINIDKAMDVIGIAKKYNLKIAGLNQHIGSFIMTCTEYIKGVKELLHLSLLFE
                     DLEFLDFGGGFGIPYNKQQGQQPLDLEKFGKELKDVLTAWQEEHNKTILFKCEPGRFV
                     VAESSILLGKVYAKKHNYNKIYIGTDIGFSVLIRHAMYGAHHDIEIYRDDKPVKNGAY
                     EYVNIAGNICESGDILAEERQLPVILEDDIIGVMDAGAYGYSM"
BASE COUNT          667 a          368 c          505 g          510 t
ORIGIN      
        1 tgcgacgcaa aatcaacctg tcagatatca cggaaggaaa ccggagtttg attttgctat
       61 aaaagaccat gttgctttat gtgaagcact gggcataata gattataaac gcggagccaa
      121 actcagcgga agcggccact ggatgtacag aggtgacgga gcacttttag agtgggcttt
      181 gcttaactat ttcataaccg agcatatcaa agacggatat gagttcattc tgcctcctca
      241 tatactcaat tccgaatgcg gatatacagc gggacagttt cccaaattcg gtgatgatgt
      301 atttaaactt accgaaaatg atcagttttt actgccgacg gcggaaaccg cactgataaa
      361 tctgcacagg aatgaagttt tgagagaaga agagcttccc aaaaaatact ttgcttatac
      421 tccctgttac agaaaagaag cgggcagtta cagagcggaa gaaagaggta tgattagagg
      481 tcatcagttc aataaagtgg aaatgttcca gtataccgtg ccggaaaaaa gcgaagaagc
      541 tctgatggaa ctggtagcca aagcggagac tctgatgaac ggattgggct tacatttccg
      601 cttaagtaaa ctggctgcag ccgactgttc tttcgcaatg ggcaaaacat atgatgtgga
      661 agtatggatt cccagcatgg gtatatacaa ggaagtaagt tccgcttcca atgcatatga
      721 ctttcaggct atcaggggag caatgaggtt taagagaacc gcaaccggga aaaatgagta
      781 cctgcataca ttaaatgctt cgggacttgc cacttccaga gtgtttccgg caattataga
      841 acagaatcag cataaagacg gatcggtgac tgttcccgaa gtattaagaa aatgggtagg
      901 taaagataaa ttaacggtat gatagaatat ttcagcgaaa aaacggattt ttataaacac
      961 cacaagcctt cggagctgct ctcggcatac gggtcgcctt tgtatgttta caatgaagat
     1021 atactgagag agcgggcaag gcagataaag aattttgtga atatgcccaa ctttaccgtt
     1081 tcctattcgg taaaggcaaa ctgcaatctg catattctga agatattaaa agaagaagga
     1141 ctgcatgccg atgccatgag cgaaggagaa atctttgtaa cggaacaggc cggttacgaa
     1201 aaagaccgta tattcttcgt atgcaacaat gtatcggccc gggaaatgaa atatgccgta
     1261 gacagaggta ttatggtaag tgtggattca ctgtctcagc tggaactgta cggaaggaac
     1321 tttcccaaga ctaaagtagc ggtaaggata aatcccggta taggagcggg acatcataag
     1381 aaagtggtta cagccggaga ggctaccaaa ttcggtatta atatcgataa agcgatggat
     1441 gtcataggta ttgcgaaaaa gtataatctg aagattgcgg gtttaaacca gcatataggt
     1501 tctttcataa tgacttgtac cgaatatatc aaaggcgtaa aggaattatt gcacttaagt
     1561 ttgctttttg aggatctgga gtttctggat ttcggcggcg gattcggtat tccgtataat
     1621 aagcagcagg ggcaacagcc gttggatctt gaaaaattcg gaaaagagct taaggatgtt
     1681 ttgaccgcat ggcaggaaga gcacaataaa accatactgt tcaaatgcga gccggggcga
     1741 tttgtagtcg cggaatcaag tatactttta ggcaaggtgt atgcaaaaaa gcataattac
     1801 aataaaatat acatcggaac cgacataggc ttttccgtac tcatccgtca cgcaatgtac
     1861 ggtgctcacc atgatataga aatatacaga gatgacaaac ccgttaagaa cggtgcatat
     1921 gaatatgtca atattgcagg taatatatgc gaaagcggag atatacttgc cgaagaaagg
     1981 caattacccg tgatattgga ggacgacatc ataggtgtta tggatgcggg agcttacgga
     2041 tattccatga
//
LOCUS       DAOPWC010000293         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_59234_length_2044_cov_3.238311, whole genome shotgun sequence.
ACCESSION   DAOPWC010000293 DAOPWC010000000
VERSION     DAOPWC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59234_length_2044_cov_3.238311"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKJ65_06330"
     CDS             <1..467
                     /locus_tag="PKJ65_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ41486.1"
                     /translation="GIHGLNPVMTKGISEELIYKIYISALTTINIDSHNRFATTDTRL
                     IRRLTRDYEYRNSSASRTLNMWESVRSGEDKYIFPFQEQADVIFNSALMYEHGLLRVF
                     AEPILKKVKKDDPAYDEAKRLLDILSAFEKIDSSAVPPSSIIREFIGGSCFH"
     gene            complement(468..815)
                     /locus_tag="PKJ65_06335"
     CDS             complement(468..815)
                     /locus_tag="PKJ65_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ41487.1"
                     /translation="MAQAIGPYSQAVRADNLLFVSGNLPVDPATGKIPEGVEKQTHTV
                     LGNIATLLKGAGLDMSDIIKTTLFIKDMNDFSVINAIYAEYFTSDYPARSTVEVARLP
                     KDALIEIECIAKY"
     gene            949..>2044
                     /locus_tag="PKJ65_06340"
     CDS             949..>2044
                     /locus_tag="PKJ65_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNZ41488.1"
                     /translation="MDILSDIKHKDILQVSFGKATADMSKAGIRRVNDGFQLEYFSDN
                     KAFHINFDESNLFEHIYKLITTNFKECTVYTLKNDIVIFRNRKNELIIKKHKPTKSVV
                     NTSHDKQKNHIIKDGTDAPFLYDLGISDINGVVTDKGRRKFVQINSFLQIFDHAFEKL
                     SFDNKQLKVVDFCCGKGYLTFALHFYLHNIKHMNTNITGIDLKSDVIDNLNNIAKKYG
                     MTDLTFITGDIKAYDDYSDIAVGLHACDIATDIFLSSVVRNKAKLIISVPCCQHQLFK
                     QIENEQLKPMLSYGLQKDRFTEMLTNTLRVLALKSKGYSTDMVEFAPVEHTMKNVLIR
                     ATYTGKPDKDAKEEYNRLKTMFSITDFEGDT"
BASE COUNT          692 a          350 c          390 g          612 t
ORIGIN      
        1 aaggtataca cggacttaat ccggtcatga caaaaggtat cagtgaagag ttaatttaca
       61 agatatatat cagcgcatta actacgatta atatagacag tcacaacaga tttgccacta
      121 ccgatacaag actcatacgc aggttaacca gagattacga atacaggaat tcctcggctt
      181 cccgtacatt gaacatgtgg gaatcggtca gatcaggtga ggataagtat attttcccgt
      241 tccaggaaca ggccgatgtg atattcaaca gtgcattgat gtacgaacac ggattgttaa
      301 gagtattcgc ggaacctata ttgaagaaag tcaagaaaga tgacccggca tatgatgagg
      361 cgaaaagact gttggatata ctgtcggcat ttgaaaagat tgattcttcc gcggtacctc
      421 ccagttctat tataagggag tttatagggg gctcctgctt ccattaatca atatttagct
      481 atacactcta tttctatcaa agcatccttg ggaagccttg ccacttccac agtggaacgg
      541 gcagggtagt cggatgtgaa atattcggca tatattgcat ttatgacgga aaaatcattc
      601 atatctttaa taaacaatgt tgtcttgata atatcgctca tatccagacc tgcacctttt
      661 aataaggttg ctatgtttcc aagcaccgta tgggtctgct tttccactcc ttccggaatc
      721 tttcccgtgg caggatcaac agggagattg ccagatacaa aaagcagatt atccgctctt
      781 accgcctgag aatagggccc tattgcctga gccatccttc tacttttata atcttctttt
      841 ttaaatccga tgtatttttc atatcaatac cgcctttaaa taatctctat aattcagtac
      901 ccctatttta tcatataatc ataagttaaa agatgatata attcgcatat ggatattttg
      961 tctgatataa aacataagga tattctacag gtctcattcg gaaaagcaac tgcggatatg
     1021 agtaaagccg gaataagaag agtgaatgac ggctttcaac tggagtattt ttccgataat
     1081 aaagcttttc atatcaattt tgatgaaagt aatctatttg aacatatata taagcttatt
     1141 acgacgaatt tcaaagaatg taccgtatat actttaaaaa atgacatagt tatattcaga
     1201 aacagaaaaa atgaacttat cataaaaaag cataagccta caaagtcagt tgtaaataca
     1261 agtcatgata aacagaagaa tcatatcata aaagacggaa ccgatgcacc gtttctttat
     1321 gatctgggga tatctgatat aaacggtgtt gttaccgaca aaggtcgcag aaaatttgtg
     1381 cagattaaca gttttttgca gatattcgac cacgcatttg aaaaactgtc atttgataat
     1441 aagcaactta aggttgtgga tttttgttgc ggtaaaggtt atctgacttt tgcattacac
     1501 ttttatttgc ataatattaa gcatatgaat accaatataa caggtataga tttaaagagt
     1561 gatgttatag ataatcttaa taacattgct aaaaaatacg gtatgacaga tttaactttc
     1621 ataaccggcg atattaaggc atatgacgat tattcggata tagcggtagg acttcatgca
     1681 tgtgatatag ctacggatat tttcttaagc agtgtagtaa ggaacaaggc aaaactcatc
     1741 atatcggttc cgtgttgtca gcatcagtta ttcaagcaga tagaaaatga acaacttaaa
     1801 cctatgcttt catacggttt acagaaagac aggtttaccg aaatgctgac aaatacgtta
     1861 agggttttag ccttgaaatc caaaggttac agtacggata tggtggagtt tgcacctgtt
     1921 gaacatacaa tgaagaatgt tcttataaga gcaacatata ccggtaaacc ggataaagat
     1981 gcaaaagaag agtataaccg tttgaaaact atgttttcaa taacggattt tgaaggagat
     2041 acta
//
LOCUS       DAOPWC010000294         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_59249_length_2044_cov_2.493213, whole genome shotgun sequence.
ACCESSION   DAOPWC010000294 DAOPWC010000000
VERSION     DAOPWC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59249_length_2044_cov_2.493213"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKJ65_06345"
     CDS             <1..637
                     /locus_tag="PKJ65_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41489.1"
                     /translation="GSLGDIYYAKATYLRRSGCPGGWFKDIKYAGGGPLIDLGVHVID
                     LVRYLADTPKPVSAYGATYDNLGPNRAEGPKGYVVEESSKYVFSVEDMATGLVRFDNG
                     LTMFVEASFNLNIKQDTGVIEIFGTKGGARIDPGIEFYLDINGRFVDVKPHGDSALSF
                     TGLFESEIEHFADCVINNTPCRATAEDGVMVMKIIDAIYESAKLKKEVKID"
     gene            688..1365
                     /locus_tag="PKJ65_06350"
     CDS             688..1365
                     /locus_tag="PKJ65_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ41490.1"
                     /translation="MNKILVVDDDQNICELLTLYLEKEGYTVVCANDGLEALEVFKDE
                     NPDLIILDIMLPGMDGKDVCKQIKRKDDVPILMLSAKAETFDKVLCLELGADDYVVKP
                     FEAKELVARVKAIIRRAKGNQSGYSDLKYTDLVISKNTYTVLLKDKQIELPLKEFELL
                     FLMASNPNSVFTRDMLLEKIWGFDFFGDSRTVDVHIKRLREKINLPDVNWQLKTVWGV
                     GYKFEVL"
     gene            1362..1799
                     /locus_tag="PKJ65_06355"
     CDS             1362..1799
                     /locus_tag="PKJ65_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41491.1"
                     /translation="MKRNVLSATLRRFVLYIVIIVSLFSVIIYYQTWQSILNNQSLTA
                     MNIYPLVKNELDQRKVLLYSNLTSSAFNAQTYLFDSIDKLAAMSNKMIWIIDEEGKVV
                     SHAPRMESLIKILKKRQETFTYHLPEDTGYKNIRFRFGIYQKQ"
BASE COUNT          643 a          324 c          473 g          604 t
ORIGIN      
        1 cggatcattg ggagatattt attatgcaaa agccacatac ttacgtcgca gcggttgtcc
       61 cggagggtgg tttaaggata taaaatatgc aggcgggggt ccgttgatag acctgggtgt
      121 tcatgtaata gatctggttc gttatcttgc agatactccc aagccggtat cggcatacgg
      181 agcgacatat gacaatctcg gtccgaacag agcggaggga cccaaaggat atgttgtcga
      241 agagagttca aaatatgtat tcagtgtgga agacatggct acgggactgg tcagatttga
      301 taacggactt acaatgttcg tggaggcttc gtttaatctg aatattaagc aggataccgg
      361 tgtaatagag atattcggga ccaaaggcgg tgcaagaata gatcccggta tagaatttta
      421 cctggatatc aacggcagat ttgtggatgt caaaccgcat ggtgattccg cattgagctt
      481 taccggtctt tttgaaagtg agatagagca ttttgcggat tgtgttatca ataacacacc
      541 gtgccgtgcc acagcggaag acggtgtaat ggtaatgaag attatagatg cgatatatga
      601 gtcggcaaaa ttgaaaaagg aagtgaaaat tgattagttt ggtgcacata atattcaaac
      661 tgctcaattt agtaaagggt aatactgatg aacaaaattc tggttgttga tgatgatcaa
      721 aacatatgcg agcttctgac tctctatttg gaaaaagaag gttatacggt agtatgtgcc
      781 aatgacggat tggaggcttt ggaagtgttc aaggatgaaa atcccgattt gattattctg
      841 gatatcatgc ttccgggaat ggacggtaag gatgtctgca aacagataaa gagaaaagac
      901 gatgtgccta ttcttatgtt aagtgcaaaa gccgagactt tcgacaaggt tctatgtctg
      961 gaattgggtg ccgatgatta tgtcgttaaa ccttttgagg caaaagagct ggttgcaaga
     1021 gtaaaagcta ttatacgaag ggctaaaggt aatcaaagcg ggtattccga tttaaaatat
     1081 accgatttgg tgataagtaa gaatacctac acggtgttgc tcaaagataa acagatagaa
     1141 cttcctctta aagaatttga gttgctgttt ttaatggctt ccaatcccaa ttcggttttc
     1201 acgagagata tgcttttgga gaaaatatgg ggatttgact ttttcggaga ttccagaaca
     1261 gtcgatgtac atataaaaag gctgagggaa aaaataaatc tccccgatgt aaactggcag
     1321 ttaaagaccg tctggggtgt aggatataaa tttgaagtat tatgaaacga aatgttttat
     1381 ccgcaacatt aagaagattt gtgctttata tcgttattat agtatccctt ttttcggtta
     1441 taatctatta tcagacctgg caaagcatac ttaataatca gagtctgact gcaatgaata
     1501 tatatccttt ggttaaaaac gaactggacc agagaaaagt gcttctgtat tccaatctta
     1561 cttccagtgc ttttaatgcc cagacatatc tgtttgattc cattgacaag cttgccgcaa
     1621 tgagcaacaa aatgatatgg attatagatg aagagggaaa ggttgtttct catgctccca
     1681 gaatggagtc tttaattaaa atattgaaaa agaggcagga aaccttcaca tatcatcttc
     1741 cggaagatac gggttataaa aatattcgat tccgattcgg catatatcaa aaacagtaaa
     1801 atttttaatg atattcttga agaagaggat tttatcggaa ggtcatatat atttgttgac
     1861 acttacaata tttcgaggag cgatcccgaa aaggtgaaac gtattgcggt gtgcataagt
     1921 acatataaac aacaggaaaa agaagcattt ctttcaggta tacatcttct tgtttttccc
     1981 attgcgttta tttccatatt atcggttttc ctggtagtgg ctttcgccaa atctatatcc
     2041 ggac
//
LOCUS       DAOPWC010000295         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_59419_length_2040_cov_3.203526, whole genome shotgun sequence.
ACCESSION   DAOPWC010000295 DAOPWC010000000
VERSION     DAOPWC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59419_length_2040_cov_3.203526"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKJ65_06360"
     CDS             <1..770
                     /locus_tag="PKJ65_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41492.1"
                     /translation="DVVMTDVFSSVFLQSIDLTGDSIDTPEGYNIKRYGVFDGRYGIY
                     KQRINPLVTDVESEIAVYHLAKRLGVPCCPATRADKDTVFSTFLYDFSKEYIVHFRRL
                     LKGERSDNEYRNLIEVRPKFKEDIARMILLDFITRQDDRHLSNMAIKISGNRESFYPL
                     YDNGRSLFYEDTEETVEKAIKDIAGYSTVFGYSGTYWEHVQDIAAKMNIADLINLNIT
                     KAEVEIILKQAGFVSYRLEGATEWVVKSINMLKEINR"
     gene            1001..>2040
                     /locus_tag="PKJ65_06365"
     CDS             1001..>2040
                     /locus_tag="PKJ65_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41493.1"
                     /translation="MKKKKFIVCFTAVLILITVFSVTLLTGSEAENHISINGIPQEIE
                     MMQGVTGSNDSPLVKMTTSEDLQRIYDSMDPITGQPTYLPPVMVKLSEDEEYQVAERC
                     TLTCVIDENTSYDLKFKYVVRDGKKILIHGGEVYSSFEIPNSDNYAIACGGLIYLLDL
                     NEGTLVMYNSKEAGGYSFYTYNKTIYRKVWASKPSFNPSGTKMLYYTERANNEVGSIW
                     VMDNETKTEKPIPNTTAYNRVLQWVNDDVAYIKTAYEVLRIDTSKYTSQSIYSDNGNP
                     LCDTVLSYPYMFVSDLKGSRIYNLEDGSIREYDDSRYSHCAAAVSGFGNMALLVYGMP
                     KDDVWLSFMKTY"
BASE COUNT          669 a          327 c          452 g          592 t
ORIGIN      
        1 tggatgttgt gatgacggat gtgttctcgt ccgtctttct tcaaagtatt gatttaacgg
       61 gagacagtat agatacacct gagggctata acattaaacg atacggcgtt tttgacggca
      121 ggtacggcat ctataagcaa cgcatcaatc cgcttgttac cgatgtggag tcggagatag
      181 cggtctatca tcttgcaaag cgtttaggcg ttccgtgctg tccggcaaca cgtgcagata
      241 aagacaccgt attttccaca tttctatatg atttttcaaa ggagtatatt gttcatttcc
      301 gccgtctgct taaaggagag cggtcggata acgagtatag aaatcttatt gaagtgcgcc
      361 ctaagttcaa agaggatatt gcacggatga ttcttctgga cttcattaca cgtcaggacg
      421 acagacatct ttcaaacatg gctattaaga ttagcggcaa cagggagagt ttctatcctc
      481 tctatgataa cggcagaagt ctgttttatg aagataccga ggaaacggtc gaaaaagcaa
      541 taaaggatat tgcaggctat tcaacggttt tcggttattc cggcacctat tgggagcatg
      601 tacaggatat cgctgccaaa atgaatatag cggatttgat taatctgaat attacaaaag
      661 cggaagttga aatcatactc aaacaggcag gcttcgtttc ctatcgcttg gaaggtgcaa
      721 cggaatgggt cgttaagtca attaatatgc tgaaagaaat aaacaggtag tggagattta
      781 cctatctgtt ctttacatgg tgaagagaaa tacatattga gaaatagagg aaaataagag
      841 taattttctt gtatttatga actttttgta aacaatgccg gtgaatttgt aaacaattta
      901 tgaacaacgg caattatgga aattaaaggc atataatata acacaagtga tgttcagctt
      961 acatatagtg tttgaaggta taaagaatgt gaggtttgat atgaaaaaga agaaatttat
     1021 agtttgtttt acggcagtac ttatactgat aaccgttttc agcgttacat tgcttacagg
     1081 ttctgaagcg gaaaatcata taagtattaa cggaataccg caagaaattg aaatgatgca
     1141 gggagtaacc ggtagcaatg atagtccttt ggtaaaaatg acaacatcag aggatcttca
     1201 gagaatctat gactctatgg atcccataac gggacaaccg acatatctgc caccggttat
     1261 ggttaaactt tctgaagatg aggaatatca agtggctgaa agatgcacac tgacatgtgt
     1321 tattgatgaa aacacttcat atgacctaaa atttaagtat gtagtgagag atggtaaaaa
     1381 gatattaatt cacggaggtg aagtatattc atcatttgaa attccgaaca gcgataatta
     1441 tgctatagca tgtggcggtc ttatatatct tcttgatttg aatgaaggaa ctcttgttat
     1501 gtataacagt aaggaagcgg ggggatattc gttctatacc tataataaaa cgatatacag
     1561 aaaagtatgg gctagcaagc cttctttcaa tcccagcgga acaaaaatgc tttactacac
     1621 ggaaagagca aataacgaag taggcagtat atgggtaatg gataatgaaa cgaaaaccga
     1681 gaaacctata ccgaatacta cagcatacaa tcgggtgctg cagtgggtta atgatgatgt
     1741 agcatatata aagacagcat atgaagtatt aagaatagat acttccaaat acacatctca
     1801 atctatttat agcgataatg gtaatccact ctgtgatacc gttctttcct atccgtatat
     1861 gtttgtgtct gatttgaaag gctccaggat atataacctg gaagatggta gcataaggga
     1921 atatgacgat tcaaggtatt cacactgtgc agctgccgtg tccggtttcg gtaatatggc
     1981 tttacttgta tacggtatgc caaaagatga tgtctggctt agttttatga aaacatattt
//
LOCUS       DAOPWC010000296         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_60120_length_2025_cov_2.504569, whole genome shotgun sequence.
ACCESSION   DAOPWC010000296 DAOPWC010000000
VERSION     DAOPWC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60120_length_2025_cov_2.504569"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..712
                     /locus_tag="PKJ65_06370"
     CDS             35..712
                     /locus_tag="PKJ65_06370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ41494.1"
                     /translation="MREVKRLLSSRLLMVSDLALPCEKVFDIGTDHCYLPIFLIQKGI
                     CKTAVATDIKEGPVRIAKRNVRLFGMEKHIDVFVADGISHVGDGCCVIIAGMGADVIV
                     DILKKDMDSAKSCEWLILQCQSKTERLRAFLWDNGFAIEEEALINDRDKIYNAFRASY
                     TGINQPYTEIDTIASEILAKRHHPLLPGYVSGYIKKLDDMIAGYREAGRDFTEEIKLK
                     RALEELL"
     gene            709..1452
                     /locus_tag="PKJ65_06375"
     CDS             709..1452
                     /locus_tag="PKJ65_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNZ41495.1"
                     /translation="MKISKIIKYLEYVAPADKSTERDNVGLLIKGKDNIRRVMVCLDI
                     TKDAAEYAVSCKADLIITHHPFIYDPLYRIDNDELITVIRNKSAVISLHTNYDAARLN
                     DVLAQKCFLRETKHIFFEDGISLGRAGITDTTDFNKYIDKIKKSLGVDTVKVTGNIPD
                     KVSAVAVGTGSSGSFSEDIKELDVDVFVTGEIKYDIIKSIAGTGPAVVELGHYESEEI
                     FVDDLSALLAGKFPSLDIFPYKKRISAYF"
     gene            1535..1879
                     /gene="rnpB"
                     /locus_tag="PKJ65_06380"
     ncRNA           1535..1879
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKJ65_06380"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          657 a          327 c          487 g          554 t
ORIGIN      
        1 ttaaatatac cgatgataat gtaaaatagg gaatatgaga gaggttaaac gacttttaag
       61 tagccgattg ctcatggtat cggatttggc gctgccgtgt gagaaggtat ttgatatagg
      121 taccgatcac tgttatctgc ccatatttct catacaaaaa ggtatatgta aaacagccgt
      181 cgccaccgat ataaaagagg ggccggtaag aatcgccaag agaaatgtcc ggctgttcgg
      241 tatggaaaag catatcgatg tgtttgtcgc tgacggaata agtcatgtgg gggatggctg
      301 ttgtgttatt atagccggaa tgggtgccga tgtcatagtc gatatactta aaaaagatat
      361 ggacagcgca aaaagttgtg aatggttaat cttacagtgt cagagcaaaa ccgaacgttt
      421 gcgggccttt ttgtgggata acggatttgc tattgaagag gaagcactga ttaatgatag
      481 agacaagata tataatgctt ttcgggcaag ttacacgggc atcaatcagc cgtacaccga
      541 aatcgacacg atagcgtcgg aaattttagc aaaaagacat catcctctgt tacccggata
      601 tgtttcgggg tatataaaaa agctggacga tatgatagcg ggatacaggg aagcaggcag
      661 agattttacc gaagaaataa aattaaaaag agcattggag gaactgttat gaaaatatca
      721 aaaataatta aatatctgga atatgttgca cctgccgata aaagtacaga gcgggataat
      781 gtcgggttac taatcaaggg aaaggacaat atcagaaggg ttatggtatg tctggatatt
      841 acaaaagatg ctgcggagta tgcggtatcc tgtaaagccg atttaataat aactcatcat
      901 cccttcattt atgaccctct ttacaggata gataacgatg aacttattac agttatacgt
      961 aacaaaagtg ccgtgatttc gcttcatacc aattatgatg ctgcaaggct gaatgatgtt
     1021 ttggcacaaa aatgcttttt aagagaaaca aaacatatct tttttgaaga cggaatatct
     1081 ttgggaaggg caggaatcac cgatacaacc gactttaata aatacattga taaaataaag
     1141 aaaagtttgg gtgtggatac ggttaaggtg accggcaata taccggataa ggtaagtgca
     1201 gtggctgtgg gaaccggttc ttcaggcagc ttttccgaag atataaagga attggatgtc
     1261 gatgtgtttg taacgggtga gataaagtat gacattatca aatccatagc gggtacggga
     1321 cctgcagtcg tggaattggg acactatgaa tcggaggaga ttttcgttga tgatttatcc
     1381 gcccttttgg ccggaaagtt cccttcattg gatatttttc cgtacaaaaa aagaatttca
     1441 gcatattttt aattaaaatt cagtgtaaaa tattttaaag gtgatgaata cacatatttg
     1501 attaaaatat aataaagtga tataattaca gccctgaccg gatcagacga tcgcggatgc
     1561 aatgcctaaa ggctgcagat gaggaaagtc cgggctccga agggcagggt gccggctaat
     1621 taccggtgaa ggtgacttca aggaaagtgc aacagagaga taccgccgta gcgatacggt
     1681 aagggtggaa aggcgaggta agagctcacc agcggtatgg taacatacct ggctatgtaa
     1741 accccacctg gagcaacacc gtataaaggg acaatgggat gcccgcccgt ctctaaaggt
     1801 ggcttgagta tggtagagat atcgtgccta gatagatgat catctaatac agaacccggc
     1861 ttatagatcc agtaaggaaa atgtgcttta aaagcacatt ttttattttg aacttatgat
     1921 gttgtattag aataaacgta atttgtaaat tttaatttta actctttcta tttcttttct
     1981 tttttgattc tggaactgag ccatacttcc aaagccgatt aagct
//
LOCUS       DAOPWC010000297         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_60536_length_2016_cov_3.333503, whole genome shotgun sequence.
ACCESSION   DAOPWC010000297 DAOPWC010000000
VERSION     DAOPWC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60536_length_2016_cov_3.333503"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKJ65_06385"
     CDS             <1..393
                     /locus_tag="PKJ65_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41496.1"
                     /translation="PEFLDKFKNEFNVLYKENITSKFQEYTQSAIPLFEIDKESSSTL
                     AEVTYNQSIIQDNSVIVTLVEDNTSDNGGFVYTTDLTDNASLVAVYRYDKLYVIFELH
                     GVSFSEIGEQTDETVVRSVAKILSDLEK"
     gene            403..1926
                     /locus_tag="PKJ65_06390"
     CDS             403..1926
                     /locus_tag="PKJ65_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ41497.1"
                     /translation="MIYSFKSFYRTPIRSIIYILLLAIAATSLCTSLAMWKYSAESVE
                     HVQDTFTTIGVLSELEFMEQTDTRSLSPQYDYSMLAEVKKKAMESKYVTSYDIREYVM
                     GYNENIHADVKQGFYENTYPYSLSIVTGVCESMQWNKATVGYKAILKIDYNDLNIIPA
                     YIKRFENPQSVEIAGTYMTADNKSPFKVGEKYIVYLMCHEYYPSHKNINGRVDKSGED
                     FYKYEEMVEYSLATMKEEFGEFKEGKPFYKVTNPDLHTAQRIETTAYDFIEKEQGIWA
                     KRVEKCKITQHSAELVLTNSMKSILMFNTNSAYIVVGRNITDEEYESGANVCIVSASF
                     ASKNNLSIGDKLSFNVYENDFLPYGIMLKKGTPGVSGFLNVEGKDRIDVWLSKGFDPD
                     RGFVGEVEYEIIGTFSTRDIVNRNEFTINNNTVFVPQKSIEGDFNTRPTVHTIKRYTD
                     KLRHTELLRTSIPGCFSVIIENGYADAFEKEMEAAGYGGIFYYFDQNYFSMHISDCR"
BASE COUNT          720 a          306 c          419 g          571 t
ORIGIN      
        1 ccggaattcc ttgataaatt taaaaatgag tttaatgttc tttataaaga gaacattaca
       61 agcaaattcc aagaatacac acaatccgct atacctttat ttgaaataga taaagaaagc
      121 agcagtacct tagcggaagt aacatataat caaagcatta tccaagacaa tagtgttatt
      181 gttactcttg tagaggataa tacctctgac aatggcgggt ttgtttatac taccgatttg
      241 acagataatg cctctttagt agcagtatac agatatgaca aactatatgt tatttttgag
      301 ttacatggtg tgtctttctc ggaaatcggg gagcaaactg acgaaacagt agtacgctct
      361 gttgctaaaa tattatccga cttggaaaaa taaaggagag tcatgatata ttcatttaaa
      421 tctttttaca gaacacctat aagaagtatc atatacatac tgctgttagc catagcggca
      481 acctcactgt gcacaagttt agctatgtgg aaatattcgg cggaaagtgt agagcatgtg
      541 caagatactt ttaccaccat aggagtattg tccgagctgg aatttatgga gcagactgat
      601 acacgctcgc tgtctccaca gtatgactat tccatgttgg cggaagttaa gaaaaaagca
      661 atggagtcta agtatgtgac atcttacgat atccgtgaat atgtaatggg gtataacgag
      721 aatattcatg cggatgtcaa gcaaggtttt tatgaaaaca catatccgta ttctttgtcc
      781 attgtaacgg gagtatgtga aagcatgcaa tggaataagg ctacggtcgg ttataaagca
      841 attctaaaaa tagattacaa tgaccttaat ataatacccg catatattaa aaggtttgag
      901 aatccgcaat cggtggaaat agcaggaaca tacatgacag cggataataa aagtccgttc
      961 aaagtgggtg aaaaatacat agtgtatttg atgtgccatg aatattaccc ttctcataaa
     1021 aatattaacg gccgtgtaga taaatcgggg gaggacttct ataaatacga agagatggtg
     1081 gaatacagtt tggcgacaat gaaggaagaa ttcggagagt ttaaagaagg taaacctttt
     1141 tataaagtta ctaatccgga tttgcatacg gcacaaagga ttgaaacaac cgcctatgac
     1201 tttattgaaa aagagcaagg catatgggca aaaagggtgg aaaagtgtaa gataacacaa
     1261 cattcggcag agttggtttt gacaaacagt atgaaaagta tattaatgtt caatacaaat
     1321 agtgcataca ttgttgtagg aagaaatatt actgatgaag aatatgaaag cggagcgaat
     1381 gtatgtattg tcagtgcttc ttttgcaagt aaaaacaatc ttagcatagg ggataaactt
     1441 tcatttaatg tatatgaaaa cgatttcctg ccttacggta ttatgttaaa aaaagggaca
     1501 cccggggtta gcggattttt gaatgttgaa ggaaaagacc gaattgatgt atggttatca
     1561 aaggggtttg atccggacag aggttttgtg ggagaagtcg aatatgagat aatagggaca
     1621 ttttcaacaa gggacatagt aaacagaaat gagtttacca ttaacaataa tacggttttt
     1681 gttccgcaaa aatccataga gggagatttc aatacccgac ctaccgtgca taccataaaa
     1741 agatacaccg ataagctcag gcacacggaa ttattacgaa ccagtatacc cggttgtttt
     1801 tccgtcatta ttgaaaacgg atatgccgac gcatttgaaa aagaaatgga ggcagcggga
     1861 tacgggggaa tattctatta tttcgatcaa aattattttt ccatgcatat atctgattgc
     1921 cggtaaaaca agctaaatca aaaaattgat ttttaaaatc taaaaaaatc atttcgtttc
     1981 tatttttcag caaatataat gcattataga aacaaa
//
LOCUS       DAOPWC010000298         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_60631_length_2014_cov_2.956611, whole genome shotgun sequence.
ACCESSION   DAOPWC010000298 DAOPWC010000000
VERSION     DAOPWC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60631_length_2014_cov_2.956611"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKJ65_06395"
     CDS             <1..577
                     /locus_tag="PKJ65_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41498.1"
                     /translation="YNLKGSSSRSSIDYEKTESIKSSDGYPINVNLSKVVINDCLWER
                     YVSTTANNNERMAHKKTVSIGDWKLTELSTVISSDDDISKEISYLITFNIFSSEPRKN
                     VGSNLGVPNPEDLTFLHVMSNGIIRLAVNGQDYGKKRCMDDLCVFSDISLEKGINYVM
                     ISWTPDGKDTNIKLDWKNINLKPESNLEFFC"
     gene            780..>2014
                     /locus_tag="PKJ65_06400"
     CDS             780..>2014
                     /locus_tag="PKJ65_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41499.1"
                     /translation="MKKVKVLSLVLCLILVCSLVFTACDKKPTETATQKPTETVTTTA
                     TETQVVEEPKEVEKPRRMDMTVLYFYGDATDNAAQKFAFKNYVTREYGIDMYFHTPAR
                     DQYIETINLQAVSGDLTGLVYLFTGYEMIAWAQEGIILALDEYLKDNETWNTIISEEW
                     KELYTWDGHVWGIPKGSDGHPSYFIRTMRGDWLDKVGMKKPDTIDAFYEVVKAFTYND
                     PDGNGENDTWGFCSRNVWLMQDIFQAHDARINHVAAVLPIWNPNKDIWEDSVLKPEFA
                     ETCLFLRKCYVEGLVHPELFSMSSTNVRNLVSNGYAGSCFYWDTWLIAWENAVKKHTP
                     TGYMVGIGAISKNITTKLNQWGGNAGGGAPYCLMANTEQPKEFINWFVTLLWGSPESF
                     FTFRYGIPQADKTGKEGFYL"
BASE COUNT          676 a          365 c          435 g          538 t
ORIGIN      
        1 ttataatttg aagggaagca gttcccgttc gtctattgat tatgaaaaaa ccgaaagtat
       61 aaaaagcagt gacggatatc cgataaatgt aaatctttca aaagtcgtga ttaatgattg
      121 tctttgggag cgatatgtat caactaccgc gaataataat gaaagaatgg cacataaaaa
      181 gacggtaagc atcggtgact ggaagttaac cgaactcagt acagtcataa gcagtgatga
      241 tgatatttca aaggaaataa gttatttaat aacttttaat atattctcaa gcgaacccag
      301 aaagaatgtc ggaagtaatc tcggtgtacc caatccggag gatctgacat ttcttcatgt
      361 tatgagcaac gggattataa gattggccgt gaacggacag gactatggta agaagaggtg
      421 tatggatgat ttatgtgtct tttcagatat ctctttagaa aagggcatca attacgtaat
      481 gatatcctgg acaccggacg gaaaagatac aaatatcaag cttgactgga agaatattaa
      541 tctgaaaccg gagagcaatc tggaattttt ctgttaagca agcattatct caagattatt
      601 ataattaatt gtaaaaatcg ctcaaaaatc ttaaatataa ttaacaaaaa tcaatcatat
      661 cagataaagt gttaaatcga agtaaaaata ttgttaatta tgtattgcat cttccatttt
      721 taaccattat aatatatctg tcttttaaat ttgaaatttt tcaaagagga ggaaaaatta
      781 tgaagaaagt taaggtatta agtttagtgt tatgtttaat ccttgtctgc tctttggtgt
      841 ttacggcatg cgataagaaa cctaccgaaa cagctacaca gaagccgaca gagacggtaa
      901 caacaacagc aaccgaaacc caagttgtcg aggaacctaa agaagttgag aaacccagaa
      961 gaatggatat gacagtactg tacttctacg gtgatgcaac cgacaatgct gctcagaagt
     1021 ttgcgttcaa gaactatgta acacgtgaat acggcataga tatgtatttc catacacctg
     1081 ccagagacca gtacatagag acaatcaacc ttcaggcagt atccggagac ttgacaggtc
     1141 ttgtatatct gtttaccgga tatgaaatga ttgcttgggc acaggaaggt attatccttg
     1201 ctctcgatga atatctgaaa gataacgaga catggaacac cattatttcc gaggaatgga
     1261 aagagctcta cacatgggac ggtcatgttt ggggtattcc gaaaggtagt gacggacatc
     1321 cttcatactt catcagaact atgagaggtg actggcttga caaagtagga atgaagaagc
     1381 ccgatacaat agatgcattc tatgaagtag taaaagcttt cacctacaac gatcccgacg
     1441 gaaacggtga gaacgacacc tggggattct gctccagaaa cgtatggctg atgcaggata
     1501 ttttccaggc acacgacgca cgtatcaacc atgttgcagc tgttctgccg atatggaatc
     1561 ctaacaagga tatctgggaa gactcggttc ttaagccgga atttgcagaa acatgcttat
     1621 tcttacgtaa gtgctatgta gaaggacttg tacatcccga attgttctcc atgagctcga
     1681 ccaacgtcag aaaccttgtt tccaacggat atgcaggatc ctgcttctat tgggatacat
     1741 ggcttatcgc atgggaaaat gccgttaaga aacacacacc tacaggttac atggtaggta
     1801 tcggtgcaat ctccaagaac attacaacca aactcaatca gtggggcggt aatgcaggcg
     1861 gcggagctcc gtattgctta atggcaaata ccgaacagcc caaggaattc atcaactggt
     1921 ttgtaacatt attgtgggga tcacctgaat cgttctttac attcagatac ggtattccgc
     1981 aggcagacaa gaccggaaaa gaaggattct atct
//
LOCUS       DAOPWC010000299         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_61009_length_2006_cov_2.670938, whole genome shotgun sequence.
ACCESSION   DAOPWC010000299 DAOPWC010000000
VERSION     DAOPWC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61009_length_2006_cov_2.670938"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKJ65_06405"
     CDS             <1..328
                     /locus_tag="PKJ65_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ41500.1"
                     /translation="GGSSYIPPMPIESVVKENIQAILQADIPSDEKAIKLCLYYMKAQ
                     IFNDGNKRAAIIFANHYLISQGGGIIIVPEKNVPQFKTLLIAYYEDRDKGEITEFMKK
                     QCIKTF"
     gene            536..1453
                     /locus_tag="PKJ65_06410"
     CDS             536..1453
                     /locus_tag="PKJ65_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ41501.1"
                     /translation="MKEGKIMEGSNLFYDQLPSGDEQLSFTYLEKKLREIIKIPTLDN
                     DILRSLGFYTKDMKYNNAASIFADKNNMFGVDIVRFGNSINEILDRETIYKNCILEQY
                     DRALILYRKYYQYEVIGGALRERRERLPESAFREALANALVHRDFDVNAHIRIAMFKN
                     KIEISSPGGLPSSIKKEDYLNGTISVLKNPIIGNIFFRLDLIETLGTGIRRINQAYRD
                     YDIKPDYMIFDTSITVELPTTTVEYEITGEESDIVDALRGGRILTCNEITDKTEYNKS
                     KVLRLMKNLTDKKYVSVIGGGRSTKYKLR"
     gene            1635..1958
                     /locus_tag="PKJ65_06415"
     CDS             1635..1958
                     /locus_tag="PKJ65_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNZ41502.1"
                     /translation="MKNEKITRTTIHIGQKPNDAQIKEIQSAALRPVVADDDAPELTL
                     LQYAEMAAIAKERKSQKIKPVIALRISPETLEKAKATGKGYTGFLSRLLDNAINNPDI
                     VAKSL"
BASE COUNT          701 a          318 c          413 g          574 t
ORIGIN      
        1 agggggctca agctatattc ctcccatgcc gatcgaatcg gttgttaagg aaaatataca
       61 ggctatattg caagcggata taccgtccga tgagaaggca ataaaacttt gtctttatta
      121 catgaaagca cagatattca atgacggtaa taaaagagcg gctatcatct ttgcaaacca
      181 ttatctcata tcccaaggcg gcggaattat tatagtgccg gaaaagaatg ttccccaatt
      241 taagacattg cttattgcat actatgaaga cagagataaa ggtgagatta ccgaattcat
      301 gaaaaagcag tgtattaaaa ccttctgatc ttaagcgggc attcttaatt taaaaggcat
      361 acaaaaataa cttactgtca tgtccgtgta gagtatgcat ttttttatta ctaaagaata
      421 ccgaactgca acgaatagca tatattcgct ctaaaagtgc aacgcaaatg caacgaataa
      481 ttatttgttg catatttgtt gcattgttta tataatactt catagaaagt gccgtatgaa
      541 agaaggtaag ataatggaag gttccaattt gttttatgat caattaccgt ccggtgatga
      601 gcagcttagt tttacatatc ttgaaaagaa attaagggag attattaaaa ttcccaccct
      661 ggataatgat atactgcgtt ctttaggttt ttatacaaaa gatatgaagt ataacaatgc
      721 agcttcaata tttgcagata agaataatat gttcggtgtg gatatcgtcc gtttcggcaa
      781 ttcaattaat gagattcttg acagagaaac aatttacaaa aactgcattc tggaacagta
      841 tgacagagca ttgatattgt acagaaaata ttatcaatat gaagtgattg ggggagcatt
      901 acgtgaaaga cgtgaacgac ttcccgaatc cgcattcaga gaggcacttg caaatgcttt
      961 ggtacacaga gattttgatg taaatgcaca tataaggata gcaatgttta agaataaaat
     1021 agagatttct tcccccggag gtcttccttc ttcaattaaa aaggaagatt atctgaacgg
     1081 cactatctct gttttaaaga atccgataat agggaacatc tttttcagat tggatttaat
     1141 tgaaactttg ggaacgggaa taaggcgcat aaaccaagca tacagagatt atgatataaa
     1201 gccggattat atgatatttg ataccagtat tacggttgaa cttccgacaa ctaccgtaga
     1261 gtacgagatt accggcgaag aatcagatat cgttgatgct ctgagaggcg gtaggatttt
     1321 aacatgcaac gaaatcacgg ataagacgga gtataataaa agcaaggttc tgaggttgat
     1381 gaaaaattta accgataaga aatatgtctc cgttatcggt ggcggaagaa gtacaaagta
     1441 taaactgaga taatctgaga gaagcattaa ctgcttttaa agcggagaat tatttgctat
     1501 tgaaaaagta atactttaag ttatacttta agtatgtaag ttgcaagtac tgaaagcttg
     1561 ttgagcggaa tgcatagaag ctgtacgttt aatatctgca cgtatggcaa cagcaagaga
     1621 aaggggaatt tactatgaaa aatgaaaaga taactcgtac aactattcat atcggtcaaa
     1681 aacctaacga tgctcagatc aaagaaatcc aatcagccgc cttaagaccg gttgttgcgg
     1741 atgacgatgc tccggagctg acccttttgc aatatgcaga gatggctgct attgcaaaag
     1801 aacgcaagtc tcaaaagata aaacctgtta ttgcacttcg tatttctccg gaaactttgg
     1861 agaaagcaaa agcaacaggt aaaggctata cgggtttttt aagccgtctg ttggataatg
     1921 ctattaacaa tcctgacatt gttgcaaaaa gtttatagct ctttagagat aagtaaattg
     1981 tacatatgct gaaacagttt gattcg
//
LOCUS       DAOPWC010000300         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_61047_length_2005_cov_3.010769, whole genome shotgun sequence.
ACCESSION   DAOPWC010000300 DAOPWC010000000
VERSION     DAOPWC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61047_length_2005_cov_3.010769"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /gene="feoB"
                     /locus_tag="PKJ65_06420"
     CDS             complement(<1..1502)
                     /gene="feoB"
                     /locus_tag="PKJ65_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNZ41503.1"
                     /translation="MIIALAGNQNSGKTTLFNQLTGSNQRVGNFPGVTVDVKEGIYKA
                     DKSIKVIDLPGIYSLSPYSPEEILTRNYLIKQRPDGIINIIDGTSLERGLYLSLQLLL
                     LNIPMVIAINMMDEVYVNGIVIKTDILSEYLKVPIIPVCANKNEGIADLMQAMTDLVK
                     NRCTPPKQDFCTGAVHRTLHATAHILEDHAQRANVPASFAAVKIIEGDEPMMNDLALG
                     KNEIEMIEHAITEMENELSLDRMAAIADMRYQYIEDLCKKAVTRPKCEKHHDRTIKID
                     NVLTHKIFGIPIFLGIMLLIFWLTFGVVGSFLSDLLSGAIDSLAQIVSKGLTDFGINP
                     VVESLVIDGVFAGVGSVLSFIPTIIVLFLLLSLLEDSGYMARVAFIMDKLLRKIGLSG
                     KSIVPMLIGFGCTVPAIMATRTLTSDRDKKMTVFLTPFMSCSAKLPIYAVFTMAFFTE
                     HRALVMTAMYLIGILLAIITALISKKFLFKGNPIPFVMELPNYRLPTVRNT"
     gene            complement(1499..1729)
                     /locus_tag="PKJ65_06425"
     CDS             complement(1499..1729)
                     /locus_tag="PKJ65_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HNZ41504.1"
                     /translation="MTLDKLGVGKSGRIIAVRGHGRLRHRLLDMGLTPRVIVHMQRKA
                     PMGDPVCIYLRGYELTLRLEDASMIDIEEISV"
BASE COUNT          560 a          434 c          416 g          595 t
ORIGIN      
        1 gtattcctta ccgtgggcag tctgtagttg ggaagctcca ttacaaaggg aatgggattt
       61 cctttgaata ggaacttttt ggatatgaga gcagttatta ttgcaagcaa tatacctatt
      121 aaatacattg cggtcataac caaagcgcga tgttcggtga aaaaggccat tgtaaaaacc
      181 gcatatatgg gcaactttgc cgaacagctc atgaaaggag taagaaaaac cgtcatcttt
      241 ttgtctctgt cggaagtcaa tgtccttgta gccattatgg ccggtaccgt acagccgaaa
      301 cctatcagca tgggaactat actttttccg gacaacccga tttttcgtag cagtttatcc
      361 attataaatg caactcttgc catatatccc gaatcttcca gcaatgataa aagaagaaac
      421 agaacgataa tggtcggtat aaaggaaagt acacttccca ctcccgcaaa aactccgtca
      481 attaccagtg actccacaac gggatttata ccgaagtcgg tcaggccttt tgaaactatc
      541 tgagccaaag agtcgattgc tcccgacaaa aggtcggaaa ggaagctgcc cacgactccg
      601 aatgtcagcc agaaaatgag cagcattatg cctaagaata tgggaatacc gaatatctta
      661 tgagtcagca cgttatcaat cttaatagtc ctgtcatggt gtttttcgca tttggggcgg
      721 gtgactgctt ttttacataa atcctctatg tactgatatc gcatatcggc aattgccgcc
      781 atcctgtcta aggatagttc gttttccatt tcggttatag catgctctat catctctatt
      841 tcatttttcc ctaaagcgag atcgttcatc atgggttcat cgccttcaat aatcttaaca
      901 gccgcaaaag atgcgggcac atttgctctt tgggcatggt cttccagaat atgtgccgtt
      961 gcatgcaaag tcctgtgaac cgcacccgta cagaaatcct gtttgggagg ggtgcatcgg
     1021 tttttcacca gatcggtcat ggcttgcata agatcggcta taccttcgtt tttattagca
     1081 catacgggta tgatgggaac ctttaaatat tcactgagga tatcggtctt tatgactatt
     1141 ccgtttacat atacttcatc catcatattt atcgctataa ccatagggat gttgagcagc
     1201 aataattgta aggaaagata aagacctctc tccagtgaag tgccgtcaat tatatttata
     1261 atgccgtcgg gccgttgttt tatgagatag ttccgcgtaa gtatttcttc gggagaatag
     1321 ggagataaag agtatatgcc cggaaggtca ataaccttta tgctcttatc ggctttatat
     1381 ataccttctt ttacatccac tgtgacgccg gggaaattgc ccacacgctg attggaacct
     1441 gtcagctgat tgaagagagt ggttttaccg gaattctgat tgcctgccag cgctataatc
     1501 atacgcttat ctcctcaata tcaatcatgg acgcatcttc cagtctcaag gtcagttcat
     1561 aacctcttaa atatatgcac accggatcgc ccataggggc tttcctttgc atatgcacga
     1621 taactcttgg ggtaagaccc atatccagaa ggcgatggcg taatctgccg tgacctctga
     1681 ccgcaattat tctccctgat ttacccacgc cgagtttgtc caatgtcata caatttctcc
     1741 atatccggta tgttctgata catgcctaaa catgtaaatt tgcataccaa tgatagaata
     1801 tcttaagatt atcggcaagt caataagcag ccgctaactc tttgaaaaat ccttttgttt
     1861 cattgacgat gtaagtcggt aagtgttgcc ttgatacaac aaaaatggca taatgtacac
     1921 atggctgtca ggctgtttcg ggtctgattt cctgtatcac ttacggtatt taataaagga
     1981 ggattggaaa tggcatataa atggt
//
LOCUS       DAOPWC010000301         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_61094_length_2004_cov_2.743971, whole genome shotgun sequence.
ACCESSION   DAOPWC010000301 DAOPWC010000000
VERSION     DAOPWC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61094_length_2004_cov_2.743971"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /gene="tyrS"
                     /locus_tag="PKJ65_06430"
     CDS             complement(<1..906)
                     /gene="tyrS"
                     /locus_tag="PKJ65_06430"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNZ41505.1"
                     /translation="MKAYDILKERGFIAQATDEQAIRNLLNNERVVFYIGFDPTADSL
                     HIGHFLQLIVMSHLQKAGHKPIALLGGGTAMIGDPTGKTDMRRIMTREEIDYNASCFK
                     KQMSKFVDFTNDRAIMVDNYEWLSKLEYIPFLREIGMHFSVNRMLTAECFKSRLEKGL
                     SFIEFNYMLMQSYDFLLLRRKYGCVLQLGGDDQWSNIISGVDLIRRKEMGEAYGMTFN
                     LLTTNEGKKMGKTEKGAIWLDENKTSPFDFYQYWRNIDDAEVIKCLKLLTFLPMEEIN
                     KLGKLKDAEINKAKEVLAYEVTKIVH"
     gene            complement(919..>2004)
                     /locus_tag="PKJ65_06435"
     CDS             complement(919..>2004)
                     /locus_tag="PKJ65_06435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNZ41506.1"
                     /translation="STNAMKEGHSMSERAVGDSLDNLFRKASSRIIVASFASNIHRIQ
                     QVLNLAEKYKKKVCVFGFSMRNNIKKSVELGYIQVKRGVIIEDFEIKNYKPKDMVFIV
                     SGSQGEANSALSKIAFMKHPKINIEKNDLVIISASVIPGNEKYLYRVINELFKNGANV
                     IYQALNEVHASGHAYKQELRLLLTLVRPKYYMPVHGEFMHMESNALLAMELGIKEKNI
                     FLLENGDVLNVYSNRITVNEDAKINVGSSFVDGHGVGELGDDVLKERVILASDGILNV
                     SIVLNKFDRKLAAQPVIIPVGFIYESESDDIIRKIRIILDEDIKKYTTTSMSINELQN
                     SVKKRLRTFLYQETKRNPVVLPVIMEV"
BASE COUNT          539 a          462 c          305 g          698 t
ORIGIN      
        1 gtgaactatt tttgtaacct cataagccag aacttccttg gccttattta tttccgcatc
       61 tttcagctta cctaacttat ttatttcctc cataggtaag aaggtaagaa gtttcagaca
      121 cttaataact tccgcatcgt ctatatttct ccagtactga tagaaatcaa acggcgatgt
      181 cttattctca tccagccata tggctccttt ttccgtctta cccattttct ttccttcatt
      241 tgtagtaagc aggttgaagg tcattccgta tgcttccccc atttcctttc ttcttatcag
      301 atccactccc gatattatat tgctccattg gtcatccccg cccaattgca gtacacaacc
      361 gtatttcctt ctcagtaaca gaaaatcgta actttgcatg agcatatagt tgaactcgat
      421 aaaggataat cctttttcca atcgggattt aaaacattcc gcagtaagca ttctgttaac
      481 ggaaaaatgc attcctattt cccttaaaaa cggtatatat tccagttttg acagccattc
      541 gtaattgtcc accataatgg ctctgtcatt ggtaaaatcc acaaacttcg acatctgctt
      601 cttgaaacaa cttgcgttat agtcaatttc ttctcttgtc attattcttc tcatatcggt
      661 tttgccggta ggatcgccta tcatagcggt accgcctccc aacagtgcga tgggcttatg
      721 ccccgctttc tgcaggtgac tcataacaat aagctgaaga aagtgaccta tatgcagact
      781 gtccgccgta gggtcaaaac ctatgtagaa aacaactctt tcattattca gaagatttct
      841 gattgcctgt tcatcggttg cctgggctat aaagcctctt tcttttaaaa tatcatacgc
      901 tttcatttta ttttccacct atacttccat aataaccggt aatacaaccg gatttctttt
      961 tgtttcctga taaagaaaag ttcttaaacg ctttttaacg gagttctgca attcgttaat
     1021 actcattgat gtggtcgtat acttcttaat atcttcatca agtattattc taatctttct
     1081 tattatatca tccgattcac tctcgtatat gaatcctaca gggattataa caggttgtgc
     1141 tgctaatttt ctgtcaaatt tattgagcac tattgataca ttcagtattc cgtcacttgc
     1201 aagaatcact ctttctttga gaacatcgtc acccaactct cctaccccgt gtccgtctac
     1261 aaaagatgat cctacattga ttttagcatc ctcattaacg gtaatcctgt tggaatagac
     1321 attaaggaca tcaccgtttt ccaaaagaaa aatgttcttc tcctttattc ctaactccat
     1381 cgccaacagt gcattgcttt ccatatgcat aaattcgccg tgtaccggca tatagtactt
     1441 aggacgcaca agcgtaagta aaagccgcaa ttcctgctta taggcgtgtc ctgacgcatg
     1501 tacttcatta agagcctgat aaatgacatt tgctccgttt ttaaataatt catttataac
     1561 acgatacaga tatttctcat ttcccggtat taccgaggcg gagataataa ccaggtcatt
     1621 tttctctata ttaattttag gatgcttcat aaatgcaatc tttgaaagtg cggaattggc
     1681 ttctccctgt gaaccggata ctataaatac catatctttg ggtttatagt tctttatttc
     1741 aaaatcttct atgataacgc ctcttttaac ctgtatatat cccaactcaa cggatttttt
     1801 tatgttattt cgcatactga atccgaaaac gcatactttc tttttgtatt tctccgcaag
     1861 attcagtacc tgctgtattc tgtgtatatt ggaagcaaag gatgccacaa taatacgtga
     1921 agatgctttt ctgaacagat tatccagcga atcgcctaca gctctctcgc tcatactgtg
     1981 cccttccttc atggcatttg tact
//
LOCUS       DAOPWC010000302         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_61761_length_1989_cov_2.884695, whole genome shotgun sequence.
ACCESSION   DAOPWC010000302 DAOPWC010000000
VERSION     DAOPWC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61761_length_1989_cov_2.884695"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..588
                     /locus_tag="PKJ65_06440"
     CDS             133..588
                     /locus_tag="PKJ65_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41507.1"
                     /translation="MKKTTAKTITSVILVIFLLCISLQSVTVYAFPSHSIEKNATRNN
                     NTNNTYEYFNISSGEATVYARYVGYQNITTGAQIDIKIQRKVLFTWVDVNNGQPNNTW
                     TDYVTGYQGSVTHSLQLPGRGNYRALITYTVYGYGGSPDVIDVIKYDSY"
     gene            667..996
                     /locus_tag="PKJ65_06445"
     CDS             667..996
                     /locus_tag="PKJ65_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41508.1"
                     /translation="MKRKTISIILLVVLVLGIAFTVSASCNHNWELLYEEDSTTYYYV
                     DDDYCAYDHIEHYKCTLCPVRKDMIYPQNVAHVFGPGVYVGTLPSGEQWWYHDCLNCG
                     TRIDYYE"
     gene            1091..>1989
                     /locus_tag="PKJ65_06450"
     CDS             1091..>1989
                     /locus_tag="PKJ65_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41509.1"
                     /translation="MKGKKKNKWLLRASAMLLVLLLSLCSALSYGIWNSSENYINKIP
                     EGQTTFAWLKESEFLPNSKGSTQVYPYSQLQEIYTVADSSPNVDSVDIRFQCMAHAGL
                     NTVVSDECGVSSNTSVNMPYNDSVIVGTCEAIEFKSMSQTEKSSGNIAHFGYIATIKI
                     DKNLSPVLDPDYPYSYVRVMSSQIMADFSHFFIVGSKYIINGTVSYLHTPFKEDQAPY
                     FIMTETDFSPPVTEIKFEEYMYEKIGVPKGTKITTLHTGSRDLTYCCLVAYTDLSYLS
                     YAKIDNSLDEFLSSDENSTWNTI"
BASE COUNT          664 a          340 c          382 g          603 t
ORIGIN      
        1 aataatttat aaacataaca aaaatcgcga aaaagaaaaa tattttcaaa aagttgtccc
       61 cgttttgcct ctttagattg ttatatatag tgtaaagcaa atttacatta acaaataacg
      121 gaggtacatg tcatgaaaaa aacgaccgca aaaacaatta caagtgttat tttggttatc
      181 tttcttttgt gcatatcatt acaatcagtt acagtatatg cttttccatc tcatagtatt
      241 gaaaagaatg cgacaaggaa caataatact aacaatactt atgaatattt caatatctcc
      301 tcaggtgaag caactgtata tgccagatat gtaggttatc aaaacattac taccggggca
      361 cagattgata ttaagattca aagaaaagtg ctgttcactt gggttgatgt aaataacgga
      421 caaccgaata acacatggac cgactatgtt acaggatatc agggatccgt aactcattct
      481 cttcaattac ccggaagagg aaattacaga gcattaataa cttataccgt ttacggctac
      541 ggaggaagcc ctgatgtaat agatgttatt aagtatgaca gttattaatg gaagatgccg
      601 gctattcttt catgaataag aataaggtta ttcttccaga ataatcaaga aaggaggtga
      661 aaaccaatga aaagaaaaac catatcaata atactacttg tagtattggt gttgggtatt
      721 gcttttacgg tatctgcatc ttgtaatcat aactgggaat tactgtatga agaagatagt
      781 acaacttact attatgtaga tgatgattac tgtgcctatg atcatattga gcactacaaa
      841 tgtactttat gccctgttag aaaagatatg atatatccgc agaatgtcgc acatgtattc
      901 ggtcccggtg tctatgtagg gacgcttcct tcaggagaac aatggtggta tcatgactgt
      961 ctcaactgcg gcacgagaat tgattactat gagtagcaga taataagtaa agcatacagc
     1021 agtgttatct tgcttgcagg ataacattgt tgtatatgtg gtgcaaaaac acaacaatcg
     1081 gaggtataaa atgaaaggga aaaagaaaaa taaatggtta ttaagggcat ctgctatgtt
     1141 gcttgtcctt ttgttaagtt tgtgctccgc tctttcctat ggaatatgga attcctcgga
     1201 aaattacata aataaaatac cggaaggaca aacaacattt gcttggttga aagaaagtga
     1261 atttttgcca aacagtaaag gatctactca agtctatcct tacagtcaac tacaggaaat
     1321 atatactgtt gccgattcat ctccgaatgt tgattcggtt gatattcggt ttcaatgcat
     1381 ggctcatgcc ggactgaata cagtggtgtc cgatgagtgc ggtgtttcct ctaatacatc
     1441 cgttaacatg ccctacaatg actctgtaat agttggaaca tgtgaagcga tagaattcaa
     1501 gagtatgtca cagacagaaa aaagctccgg caatatagcg catttcggat atatcgctac
     1561 cattaaaata gataaaaact tatctcctgt tcttgatccc gactaccctt actcgtatgt
     1621 aagggtaatg tcttcgcaga taatggcgga tttcagtcac ttctttattg tcggatctaa
     1681 atacataatt aatgggactg tttcctatct ccatacccct ttcaaggaag atcaggctcc
     1741 gtactttatc atgacggaaa cggatttttc gcctccggtg accgaaataa agtttgagga
     1801 atatatgtat gaaaagattg gtgtgccaaa agggacgaag ataaccacat tgcataccgg
     1861 ctctcgtgat cttacatatt gttgtttagt cgcatatact gatttgtcat atctttctta
     1921 tgcaaaaata gataattctt tggacgagtt tctttcttcc gatgaaaata gcacatggaa
     1981 cacaataat
//
LOCUS       DAOPWC010000303         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_61854_length_1987_cov_3.259834, whole genome shotgun sequence.
ACCESSION   DAOPWC010000303 DAOPWC010000000
VERSION     DAOPWC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61854_length_1987_cov_3.259834"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKJ65_06455"
     CDS             complement(<1..637)
                     /locus_tag="PKJ65_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNZ41510.1"
                     /translation="MLKTRFEDILASNDKKIEQLSEKALASDIISNELYEKYNVFRGL
                     RDNKGVGVITGLTEISEMNGYIKNEDGIRVPIEGVMYYRGIDIFEIIAGIMKNNRFGY
                     EECAYLLLCGSLPDRKELEEFVQLLNDFRSVPDSFVRDIILKAPSKDVMNAISRSILT
                     LYSYDNNPDDISIKNVFRQAVQLIAVMPMLAVYSYQAYMHYILGKNLYIRQP"
     gene            830..>1987
                     /locus_tag="PKJ65_06460"
     CDS             830..>1987
                     /locus_tag="PKJ65_06460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ41511.1"
                     /translation="MICILRVFGQEINKMRIGQFTDSFLPIVDGVGRVVYGYADALSR
                     RGHECYVITPISDAGFRGKYPFDIVDFYGVTVPKYTQYKMGIPAWDIHYHERIKTIKF
                     DIIHVHSPFRSGFEGLRLARKMKIPLVGTFHSKYYEDFLQALKVQPLAEAGAMLVGDF
                     YKKCDEVWAVSEISAEALRSYGYKGDVRIMRNGTEVRKPDRKNVNYVKSTFNLGDVPV
                     ILFVGQMNWKKNIQKVLEAVSLLDRQGYTTKLILAGQGPHTEEIRSKITELNISHIVE
                     MAGHISDVNILDGLYGVAEILAFPSLYDNSPMVVREAAAMMTPSLLVRRSSASEGITD
                     GYNGYLCEDDSKDIASVLKRVLFEEKNRVRQVGRNAQMTIPVSWDIVVEDVE"
BASE COUNT          585 a          353 c          458 g          591 t
ORIGIN      
        1 tgggttgtct tatatagaga ttctttccga gtatatagtg catatatgcc tgataactgt
       61 atacggccag catgggcatt accgctatca gctgtacagc ttgtctgaat acattcttta
      121 tggaaatatc gtcgggattg ttatcatacg aataaagggt aaggatactt ctggaaatgg
      181 cattcatgac atccttagag ggtgctttta gaataatatc tctgacgaaa ctgtcgggta
      241 ccgaacgaaa atcgttaagc agttgaacaa attcctccaa ttccttcctg tcgggtagag
      301 atccgcacaa taacagataa gcacactctt catatccgaa tcggttattc ttcataatgc
      361 cggctattat ttcaaatata tctatacctc tgtaatacat gacgccttca atgggaacac
      421 gtataccgtc ttcatttttt atatacccgt tcatttcgga tatctcggta agtccggtta
      481 ttacacctac tcccttattg tcacgtaagc ctctgaagac attgtatttt tcatacagtt
      541 cattacttat aatgtcactt gccagtgctt tttcactgag ttgttctatt tttttgtcgt
      601 tggaggccaa tatgtcctca aatctggttt ttaacactgt ttatccgtcc ttttatcgta
      661 tgtaataata acataaatca ttcttccgat aaagtttttt ataacatctc cgattctccg
      721 actccctttt ttactgattc tttatgttgc gggataattg actcggtaat tataaaggaa
      781 tataattgaa cttgtggata ctctgccgtc aggttatatg gggttaacgt tgatttgcat
      841 attaagagta ttcggacagg aaataaataa aatgagaata ggacagttta ccgattcatt
      901 tctgccgata gttgacggag tagggcgggt cgtttacggc tatgcagatg cattaagcag
      961 gagaggacac gagtgttacg ttattacacc tatttccgat gccggtttcc gcggcaagta
     1021 tccgtttgat attgtggact tctatggagt tacggtgcct aaatatacgc agtataagat
     1081 gggtatacct gcatgggata tccattatca tgagagaata aaaacaataa agttcgatat
     1141 aattcacgtt cacagcccct ttcgttcggg ttttgagggg ttgcgtttag caagaaaaat
     1201 gaagataccg ttagtcggaa cctttcattc caaatattat gaagattttc tgcaggcatt
     1261 gaaggtgcag cctctggccg aagccggagc catgcttgtg ggagatttct ataaaaaatg
     1321 cgatgaagtc tgggcggtaa gcgaaatatc ggcggaagcc ttaagaagtt acggttacaa
     1381 aggtgatgtc agaataatga gaaacggtac ggaagtgcgt aaacccgaca gaaagaatgt
     1441 caactatgta aagtcgactt tcaatttggg tgatgtgccg gtaattcttt ttgtggggca
     1501 gatgaattgg aagaagaata tacaaaaagt actggaagcg gtaagtctgc tggataggca
     1561 aggatacaca accaaactga tattggcagg gcagggaccg cacacagaag aaataaggag
     1621 taagataacg gaactgaata tatctcatat tgtggaaatg gcgggacata tttccgatgt
     1681 taatatactg gacggattgt acggagttgc ggaaatattg gcttttccgt cattgtacga
     1741 taacagcccc atggtggtaa gagaagcggc agcaatgatg acaccttcat tattggtgcg
     1801 gcgcagcagc gcatcggaag gcattaccga cggatataac ggatatttat gcgaagatga
     1861 ttcaaaagac attgcaagtg ttctgaaaag ggtgttattt gaagagaaga acagagtacg
     1921 acaggtaggc aggaatgcac agatgacaat acctgtttcc tgggacattg tagtagagga
     1981 tgtcgaa
//
LOCUS       DAOPWC010000304         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_62655_length_1971_cov_2.172756, whole genome shotgun sequence.
ACCESSION   DAOPWC010000304 DAOPWC010000000
VERSION     DAOPWC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62655_length_1971_cov_2.172756"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKJ65_06465"
     CDS             <1..1021
                     /locus_tag="PKJ65_06465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNZ41512.1"
                     /translation="GCNSKDQDFTFSRDLDNNGYWRGVKALKYVELCDYKGITVPKDR
                     HTVSDDELQSAVESLLSEQVYSIEVTDRAVVYGDTLNIDYVGRIGGVAFEGGSTGGAG
                     TEVTIGVTSYIDDFLEQLVGHSPGETFDIEVTFPDNYGKEELNGKDAVFTVTINHIVE
                     SVLPELTDAFINSTFSQTKGWTTVEQMKADMYREIQSAKIADYIQEYLKTNSTVKEIP
                     KIITDYQDTAVIKYYQDYANYYQVTLEEFLQQYLNVENQQALLEQYKEDNINVSKMHL
                     ITQAIAEKEKIKATADDVTQYFVKHMNTDDYSQYEEVYGLNYLKMTVLVQMVINYVQA
                     SVVLE"
     gene            complement(1027..1584)
                     /locus_tag="PKJ65_06470"
     CDS             complement(1027..1584)
                     /locus_tag="PKJ65_06470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HNZ41513.1"
                     /translation="MTEKEKSHAGMLYQPGDPELVADRDVTVQKLYEYNRIHPLDREA
                     KKESIKGILGKTGKNCVVEQPLFCTYGYNTTVGDNFFLNINCRLMDSGKITIGNNVFI
                     APDVCIITEVHSMIVEERIQGLEYTYPVTIGDNVWISTGAHILPGVTIGDNSVIGAGS
                     VVTKDIPANCLAVGNPCRVMRKLNE"
     gene            complement(1610..>1971)
                     /locus_tag="PKJ65_06475"
     CDS             complement(1610..>1971)
                     /locus_tag="PKJ65_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41514.1"
                     /translation="DNTTDRTHLSYINIYPRYNYRLSTICFEGFEYQDIYFNIDQLRR
                     YITEYKGDKLGNFRFFFGALEPTTIKLYDSEGNLIAKHFSETAVGTYSLEGVGFIQFV
                     GRGTIMRFNISGFASIE"
BASE COUNT          604 a          394 c          392 g          581 t
ORIGIN      
        1 aggctgtaac agcaaagatc aggatttcac tttcagcaga gatctcgata ataacggtta
       61 ttggcgcggt gtaaaggctt tgaaatatgt ggagttgtgt gattataagg gtattacggt
      121 acccaaggac agacatactg tatcggatga tgaattacag tctgccgttg aaagtttact
      181 ttccgaacag gtatattcta ttgaagtaac cgacagagcg gttgtttacg gagacacgct
      241 aaacatagac tatgtaggca gaatcggcgg agtagctttt gaaggcggtt ccaccggagg
      301 tgcaggtacc gaggtgacaa taggcgttac aagttacatt gatgattttc ttgagcaact
      361 tgtaggacat agccccggcg aaactttcga tattgaggta acttttcccg ataattacgg
      421 aaaggaagaa cttaacggaa aagatgcggt atttaccgta acaattaacc acatagtgga
      481 atcggtattg ccggaactta cagatgcttt tataaactca acattcagcc aaacaaaggg
      541 atggactacc gtcgaacaaa tgaaggcgga tatgtatagg gagatacaaa gtgccaaaat
      601 agccgactat attcaggaat atcttaaaac aaacagcacg gtaaaggaga taccgaaaat
      661 aataaccgat tatcaggaca ctgccgttat taaatactat caggattatg caaactatta
      721 ccaggtcaca ctggaggagt ttttacagca gtaccttaat gtggaaaatc agcaggcact
      781 tttggaacag tataaagagg ataatataaa tgtatccaag atgcacctta taacccaggc
      841 gattgcggaa aaggaaaaga taaaggcaac agccgatgat gttacacagt attttgtaaa
      901 acacatgaat acggacgatt attcgcagta tgaggaagta tacggattaa actatcttaa
      961 aatgaccgta ttggtacaga tggtgattaa ttatgtccaa gcttcggttg ttctggaata
     1021 aataaattat tcattcagtt tacgcataac cctacaggga ttacctactg ccagacagtt
     1081 ggcgggtatg tcctttgtaa ccacacttcc ggcacctatt acactgttat ctcctatggt
     1141 aactccgggc agtatatgag caccggtact tatccataca ttgtcgccta ttgttaccgg
     1201 atatgtgtat tccagcccct gtatgcgctc ttcgacaatc attgaatgta cttcggttat
     1261 tatacagaca tcgggggcaa tgaaaacatt atttcctatt gtaatcttac cgctgtccat
     1321 cagcctgcaa ttgatattca gaaagaagtt gtcacccacc gtcgtgttgt atccgtaagt
     1381 gcagaaaaga ggctgttcca ctacgcaatt cttccctgtt ttacccaata ttccctttat
     1441 cgattctttt ttcgcttccc tgtctaacgg atgtattctg ttatattcat acaacttctg
     1501 taccgttaca tctctgtccg ctaccagttc cggatctccc ggttgataga gcatacctgc
     1561 atgtgatttt tctttttccg tcataatatt tctccctgta aaatattcat tattcaatgc
     1621 ttgcaaaacc tgatatatta aacctcataa ttgttcctct gcctacaaat tgaataaacc
     1681 ccacaccctc gagtgaatat gtgccgactg cagtttcact gaaatgcttt gcaataagat
     1741 tgccttcgga atcatataac tttattgtcg taggctcaag agcgccaaaa aagaatcgga
     1801 aattaccgag cttatcccct ttatactcgg ttatatacct tctcaattgg tctatattaa
     1861 aatatatatc ctgatattca aatccttcaa aacatattgt acttaacctg tagttatacc
     1921 tgggatatat attgatatag gaaagatgtg tcctgtcagt agtgttatca g
//
LOCUS       DAOPWC010000305         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_62833_length_1967_cov_3.262552, whole genome shotgun sequence.
ACCESSION   DAOPWC010000305 DAOPWC010000000
VERSION     DAOPWC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62833_length_1967_cov_3.262552"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..803
                     /locus_tag="PKJ65_06480"
     CDS             36..803
                     /locus_tag="PKJ65_06480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ41515.1"
                     /translation="MSYDDGPYEDYRLVELFNKYKIKGTFHLNSRYMDRDDYPPQPEK
                     IKMTDAKKLYEGHEISVHTVNHTDLRDVNNLRFLHEVLDDKKALEDIAGYPVRGMSYP
                     YGYYNDEVVQRLRYLDMEYARTTKSHKSFYLPDDFLVWHPTCHHNDATDLISAFDSPR
                     WNMGMNLFYVWGHSYEFERWGDKWYIIEEFCKRISGREDIWYATNIEIKDYVDAVRNL
                     KFTVDTSMVYNPSCTDVWLDVDREPVKVAAGQLTKLK"
     gene            complement(796..>1967)
                     /locus_tag="PKJ65_06485"
     CDS             complement(796..>1967)
                     /locus_tag="PKJ65_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ41516.1"
                     /translation="MNKLLIKLFVKDYKNTDDASVRSAYGMLAGVSGLTVNILLFTGK
                     LFIGLLSKSIAVLADSFNNLSDAGSFLITVLASKLSKKPADKEHPFGHERIEYVAALF
                     MSFIIMNAGISLFVDSIKRITQPEPIIFEWAFVIALIVSMLLKAWVMLLNRDIGIRIN
                     SYPLKAAFRDSANDLVATSVTVISMLIAKYFNFSIDSYAGIGLSVFVFLSGLKIAKDT
                     LMPLLGSATDKKLYDRITRKVESYDGIIGTHDLIVHSYGPSNIMATIHAQVPNDGDMQ
                     KMHDIIDGIEKDVYRETGIHLVIHMDPQEVHDKRFFKYKSVVKGIVKNLDPDAAVHGF
                     NAKFDKNEINLVFDVTVAHSYTQEEETALSENIKKELAKANPQVKCDITVEHGFI"
BASE COUNT          607 a          392 c          397 g          571 t
ORIGIN      
        1 aatactttaa agacaaatta cccaaagccc ttaccatgag ttatgatgac ggaccgtatg
       61 aagattacag gcttgtggaa ctgtttaaca aatacaaaat aaagggaaca ttccatctta
      121 attcacgata catggacaga gacgattatc ctccgcagcc ggaaaagata aaaatgaccg
      181 atgcgaagaa actctacgaa ggacatgaaa tatcggtaca taccgttaat catacagatt
      241 tgcgagatgt caataatctg aggtttttac atgaagtgtt ggacgataaa aaggcactgg
      301 aagatattgc gggatatccg gtaagaggta tgtcttatcc gtacggttat tacaatgatg
      361 aagttgtaca gcgtttaaga tatttagata tggaatatgc cagaaccacc aaaagccata
      421 agtccttcta cttacccgat gattttttag tatggcatcc gacatgccat cacaacgatg
      481 caaccgacct tatatccgca ttcgacagtc ccagatggaa tatgggaatg aatcttttct
      541 atgtatgggg acacagttac gagtttgaaa gatggggtga caaatggtac ataatagagg
      601 agttctgtaa gcgtataagc ggaagagagg acatatggta tgccaccaat atcgaaataa
      661 aggattatgt cgatgctgtc agaaatctga aattcactgt ggatacttcc atggtatata
      721 atcccagttg taccgatgtc tggctggatg tggacagaga accggtaaag gttgctgcgg
      781 ggcaattaac aaagcttaaa taaaaccgtg ttctactgtt atatcacatt ttacttgagg
      841 attggctttt gccaattctt tttttatgtt ttccgaaaga gcagtttcct cctcttgggt
      901 ataggagtga gctaccgtaa catcaaatac cagatttatc tcatttttat cgaactttgc
      961 attaaagccg tgaacagcgg catcgggatc caggtttttc actatacctt taacaacgga
     1021 tttgtatttg aagaatctct tgtcatgcac ttcctgagga tccatgtgta taacaagatg
     1081 tattcccgtt tccctgtaaa catccttttc tattccgtct atgatgtcat gcattttctg
     1141 catatcaccg tcattgggaa cctgagcatg aatggtagcc attatgttcg aaggtccgta
     1201 gctgtgaaca ataaggtcgt gggtacctat tattccgtca tagctctcca ctttacgggt
     1261 aatcctgtca tacagttttt tgtccgttgc cgaacccaaa agcggcataa gagtgtcttt
     1321 ggctatcttt aatcccgaaa gaaaaacaaa taccgataaa ccgatacccg cataactgtc
     1381 tatggaaaaa ttaaagtatt ttgcaataag catggatatt acggttaccg aggtggcaac
     1441 cagatcattt gccgaatctc ggaatgcagc tttcaaagga taggagttta ttcttatacc
     1501 tatatctctg tttaacagca ttacccaggc tttaagcagc atacttacaa ttaaagctat
     1561 tacaaaagcc cattcaaata ttatgggctc cggttgagtt attcttttta tggaatcgac
     1621 gaacagcgat atacccgcat tcattatgat aaaggacata aaaagagccg ccacatactc
     1681 aatcctttca tgaccgaaag gatgctcttt gtcagcaggc tttttggaaa gctttgacgc
     1741 cagtactgtt attaaaaaag aaccggcatc ggacagatta ttgaaagaat ccgccagcac
     1801 cgcaatactt ttggataaca gaccgataaa aagcttgcct gtaaaaagca gtatatttac
     1861 agtcagtccc gatacgcccg caagcattcc gtatgcgctt cttaccgatg catcatcagt
     1921 gtttttatag tcttttacga acaactttat cagcaacttg ttcatgg
//
LOCUS       DAOPWC010000306         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_62920_length_1965_cov_3.464921, whole genome shotgun sequence.
ACCESSION   DAOPWC010000306 DAOPWC010000000
VERSION     DAOPWC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62920_length_1965_cov_3.464921"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKJ65_06490"
     CDS             <1..765
                     /locus_tag="PKJ65_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ41517.1"
                     /translation="GLQKDREYIDAVKNNRKISIVEATSGNTGIALAMICAKMGYNLI
                     ICMPDNASKERVLLLKAYGAEVVLTDAAYGMKGAIDKSLEYTDAFKLEQFKNPYNPLI
                     HEQTTAKEIFDDLKGKLDIFIASVGTGGTLTGCARYFKRQKNVHIVAVEPFESQVMAG
                     KQPGAHGISGIGAGFIPETLDMKLVDEIFPVKTESAYEWTGNLACTEGILCGVSSGAA
                     LAAAVEIAKRVENKGKNIALILPDTGERYLSTPLFE"
     gene            complement(770..1402)
                     /locus_tag="PKJ65_06495"
     CDS             complement(770..1402)
                     /locus_tag="PKJ65_06495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ41518.1"
                     /translation="MLIKKNQKVLMIGDSVTDCNRRDQNSAYLGHAYPHIVASKFYLE
                     HPDMDVEVINMGISGDTSKMLVDRWQVDVVDMEPDILTILIGINDVWRHYDTPDVTRR
                     VSKEDYIKNTRFMIESMIDKTKKIIILSPFYLEEDMSKPMKAQAHDYIVALRALVKEY
                     DVLFIDLQKEFEKLMKKYTYDQLTFKTDKIHPTMFGQYVIATLIYDTLTK"
     gene            1555..>1965
                     /locus_tag="PKJ65_06500"
     CDS             1555..>1965
                     /locus_tag="PKJ65_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41519.1"
                     /translation="MKKVICFLLIVMLVFLPSCKTVSDTDSGFDNNTETGKVFTGDIN
                     DKKFTRFRLSDHCSYSQFIETYKGIYYLVGHTVYFSSDGIAKYVKLCSKPDCMHMDDY
                     NCNAYIKTNLIAYYDGYIYCVALWEGNYWLFRMNM"
BASE COUNT          588 a          362 c          398 g          617 t
ORIGIN      
        1 ggtttgcaaa aagataggga atatatcgat gccgttaaga ataatcgaaa aataagcatt
       61 gtagaggcta ccagcggcaa tacgggcatt gcactggcca tgatttgtgc aaaaatgggt
      121 tacaacctca ttatctgtat gccggacaat gccagtaagg agcgggtgtt gctgctgaaa
      181 gcttacggag cagaggtggt gcttaccgat gccgcttatg gcatgaaagg tgcgatagat
      241 aagtctttag aatataccga tgcttttaag ctggaacagt ttaagaatcc ctataatcct
      301 ctgattcacg agcagaccac cgctaaagag atatttgatg acctgaaagg caaactggac
      361 atatttatag cctcggtagg cacgggcggt accttaacag gctgtgcaag atatttcaaa
      421 aggcaaaaga atgtgcatat tgtagcggtg gagccgtttg aatcgcaagt catggcgggt
      481 aagcagccgg gtgcacacgg tatttccggt ataggtgcgg gatttatacc tgaaacttta
      541 gatatgaaac ttgtggacga gatttttccc gttaagacgg aaagtgctta cgagtggaca
      601 ggaaaccttg catgtacaga aggcattctt tgcggagtat catcgggagc tgcactggcc
      661 gcagctgtgg aaattgcaaa aagagtggaa aataaaggga agaacattgc acttattctt
      721 cccgatacag gtgagcgata tctttccaca ccgctttttg aataacccgc tattttgtca
      781 gcgtatcata aatcagtgta gctattacat attgaccgaa cattgtggga tgtatcttat
      841 cggttttaaa agtaagctgg tcatatgtgt actttttcat aagtttctca aattccttct
      901 gcaaatcgat gaaaaggaca tcatattctt taacaagtgc tcttaaagca actatataat
      961 cgtgcgcctg agctttcatg ggcttggaca tatcctcttc aagatagaaa ggtgaaagaa
     1021 taataatctt ttttgtcttg tctatcatgc tttctatcat aaagcgcgtg ttctttatat
     1081 agtcttcttt ggacactctc cttgtcacat cgggtgtgtc ataatgtctc catacatcat
     1141 ttatccctat aagtatggtc aatatatcgg gttccatatc cacaacatca acctgccatc
     1201 tgtccaccag catttttgat gtatctcccg atatccccat gtttataacc tcaacatcca
     1261 tatccggatg ttccagataa aacttggatg ccactatatg cggataagca tgtcccagat
     1321 aagcggaatt ctgatctctt ctgttgcaat cggttaccga atcaccaatc ataagtactt
     1381 tttgattttt ctttataagc ataatttact tataccgttt catcatggat aaaacggttt
     1441 atctcctctc aaacatatta gatagattat atcacttatt tattatatta ttgccaatat
     1501 actgtagtat aatttacttg ataataacaa ttatgcggaa ataaattatt aacaatgaaa
     1561 aaagttatat gttttttatt aatagtaatg ctggtatttt taccttcctg taagaccgtt
     1621 tccgatacgg atagcggatt tgataacaac acggaaacag gtaaggtttt taccggcgat
     1681 attaacgaca aaaaatttac acgtttccgg ttatcggatc attgttccta ttcacagttt
     1741 attgaaacat acaaaggtat ttattatctt gtgggacaca ctgtctattt cagttccgac
     1801 ggtattgcaa aatatgtaaa gttgtgttca aaacccgatt gcatgcacat ggatgattat
     1861 aattgtaatg cttacataaa gacaaatttg attgcatatt acgacggcta catttactgt
     1921 gttgctcttt gggaaggtaa ttactggtta ttcaggatga atatg
//
LOCUS       DAOPWC010000307         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_62929_length_1965_cov_2.994241, whole genome shotgun sequence.
ACCESSION   DAOPWC010000307 DAOPWC010000000
VERSION     DAOPWC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62929_length_1965_cov_2.994241"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="mtnA"
                     /locus_tag="PKJ65_06505"
     CDS             <1..805
                     /gene="mtnA"
                     /locus_tag="PKJ65_06505"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNZ41520.1"
                     /translation="KTADYLDTARPTAVNLFYATKRMMDVVDNLSGTVTRPDELITAL
                     YKEAVAIHNEDIKANAEIGKNMLSLLKNAKSVLTVCNAGILATTAYGTATAAMYLAKE
                     QGRELSVYACETRPRLQGARLTSYELNKNGVDVTVITDGMVAHIMSKKMVDAFIAGCD
                     RVAANGDTANKIGTKGISIIAKRYNIPVYIACPTSTIDMNIKDGSGIVIEERDLSEVT
                     HIGGVRIVPEGVKVFNPSFDVTDHEDITAIVTEKGIAYPPFSETLKLLK"
     gene            925..>1965
                     /locus_tag="PKJ65_06510"
     CDS             925..>1965
                     /locus_tag="PKJ65_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ41521.1"
                     /translation="MLKRKATDFLNNWKSGKRKSLIITGARQVGKTFTVRMFAKEKYE
                     TFYELNFLENPELKTIFSGSLEADLILQLVRTYYADKPYVEGSTLIFLDEVQECPEAI
                     TALKFLSKDSRFDVIASGSALGMAFNQVTSFPVGYVDYLDLTALDFEEFLWAKGIEPD
                     IIDSLYKHYVDRTKVHDALHIKFLSLLNEYLILGGMPEVLQVYVDTFDYVQADAVQRR
                     LHRDYISDIARFADPNSKIKSVSCYKSIPQQLSKENRKFQYGIVEKGGTARKFESSVD
                     WLDSAHFTVTANNVSKIEYPPEHFAKDNNFKLYTTDIGMLIAMYDVRLKAAVLMERDI
                     ESQSGNLLLRTAK"
BASE COUNT          613 a          340 c          470 g          542 t
ORIGIN      
        1 gaagactgcc gactatttag atacagcaag acctaccgca gtaaatcttt tttacgccac
       61 aaaaaggatg atggatgtgg tagacaatct ttccggtacg gttactaggc cggatgaatt
      121 gataaccgct ttgtataaag aagcggttgc catacataat gaagatatta aagccaatgc
      181 tgaaatagga aagaatatgc tgtcgctgct taaaaatgca aagagtgtac tgacggtttg
      241 taatgcgggt attttagcca caacggcata cggaaccgca acggcggcta tgtatctggc
      301 taaagagcag ggaagagagc tttcggtcta tgcatgtgaa acacgaccga gactgcaggg
      361 agcaaggctt acatcatatg aactgaataa aaacggagtg gatgtcacgg ttataaccga
      421 cggtatggtg gcgcatatta tgagcaagaa aatggtggat gcgtttattg cgggctgcga
      481 cagggttgcc gcaaacggtg ataccgccaa taagatagga acaaaaggca tttccataat
      541 agcaaagaga tacaacatac cggtatatat cgcctgtccc acttccacta ttgatatgaa
      601 tattaaagac ggttccggga tagtcattga agagagggac ctttcggaag ttacccatat
      661 cggcggtgtt cgcattgttc ctgaaggtgt aaaggtgttc aatccttctt ttgatgtcac
      721 ggaccatgaa gatattacag ctattgttac ggaaaagggg atagcttatc cgccgtttag
      781 tgaaacattg aaactactga aataactgta ttagttagaa aaatatgcat acgtgcagaa
      841 aaatacatgg aaaaatatgc agcggtgcat atttttcttg aaattcgcct gtttttgtga
      901 aataatgagg gtaggaggca tgctatgtta aaaaggaaag caacggattt cttaaataac
      961 tggaaatccg gaaagagaaa aagtttaatt attacgggtg caagacaggt agggaaaact
     1021 ttcaccgtaa gaatgtttgc caaggaaaaa tatgagactt tttatgagct gaactttctg
     1081 gagaatcctg agctgaaaac cattttctcc ggttcattgg aagcggactt aatattacag
     1141 ctggtaagaa catattatgc ggataaaccg tatgttgagg gttctaccct cattttctta
     1201 gacgaggttc aggaatgccc cgaagcaata accgccttaa agtttttgtc aaaagattcc
     1261 cgttttgatg ttattgcaag cggttccgct ttaggtatgg cttttaatca ggttacatcc
     1321 tttccggtag gatatgtaga ttatctggat ttgacggctt tggactttga ggaatttctt
     1381 tgggcaaagg gaatagaacc cgatatcata gattctcttt acaagcatta tgttgacaga
     1441 accaaagttc acgacgcttt acatattaag tttctttcct tgctgaacga ataccttata
     1501 ctgggcggta tgccggaagt tctgcaggtt tatgtggata catttgatta tgtgcaggca
     1561 gatgcggtac aaagacgctt gcatcgtgat tacatatcgg atatagcgcg atttgccgat
     1621 ccgaactcga aaattaaatc ggtttcctgt tataaatcca ttccgcagca gctttccaaa
     1681 gagaatcgta aatttcaata cggcatagtg gaaaaagggg gaacggcgag gaaatttgaa
     1741 agcagtgtgg attggttgga tagtgctcat tttacggtaa ccgccaataa tgtttccaag
     1801 attgagtatc cgccggaaca ttttgcaaag gacaacaact tcaagttgta tacaaccgat
     1861 atcggaatgt tgattgccat gtacgatgtc agattgaaag cggctgtttt aatggaaaga
     1921 gatattgaat cgcaatcagg taatttactg ctaagaacgg caaaa
//
LOCUS       DAOPWC010000308         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_63067_length_1962_cov_3.199266, whole genome shotgun sequence.
ACCESSION   DAOPWC010000308 DAOPWC010000000
VERSION     DAOPWC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63067_length_1962_cov_3.199266"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKJ65_06515"
     CDS             <1..216
                     /locus_tag="PKJ65_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNZ41522.1"
                     /translation="ADEPTGNLDPKTSVEIVDLLYKVNRLGTTVVVVTHDTRIVDRLQ
                     QRVIRLQDGEIVSDTEGSIYENEACEY"
     gene            197..1090
                     /locus_tag="PKJ65_06520"
     CDS             197..1090
                     /locus_tag="PKJ65_06520"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNZ41523.1"
                     /translation="MKPVSIKYILIESFNNIVRNKVLFITSIFTVCVTILLMGVVGIL
                     LMNVSYNSDTMMEEMLNITVYVIPEADEKTLNDIQDVLVADAHVTEIKYVSQMDAYQA
                     GIELVGEEVMAELGHEFLPASFIVKLDDVTYAHIFVRNMKAMSNIYEVKYSSEAYERA
                     GAISYTIRYVCGVLTALLGILSLFLISNTIKITVSASKEEVTIMRYIGASESRIKAPF
                     YLQGGFIGFLGAIISFIIAGFGYNYMYTRLEGDLSEYMGVLELVPVPQMLLVLLGVFL
                     LYGIVLGILGSFFAIRKYLKA"
     gene            1106..>1962
                     /locus_tag="PKJ65_06525"
     CDS             1106..>1962
                     /locus_tag="PKJ65_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41524.1"
                     /translation="MIKGTEKLRKCAIILVFVLLLSFTQATFCVGDINEAKDNLGNIQ
                     DQLKKIENEIKQKQSIKSYYESLAKNMKNSISSQEIQLATYNEYIVEVTGKIDSLTET
                     IEQAEQDYAERIKVLKDRVVNAYINSNLSMLDVLMQSSSLKEYYDYIELNKLITKHDN
                     LLIEEMKVMMKDITAKRETMEMLKASYITLSEQAQIVLSNMQSMQSDALETINVTGST
                     IKVLKAKEDALEAEEAEMLNLILKLQREIEYIGGQMTWPLPSWKHIPTGNGLFGWRLH
                     PIFFEWRMH"
BASE COUNT          644 a          332 c          459 g          527 t
ORIGIN      
        1 gccgatgaac ctacgggaaa tctggatccc aaaacaagcg tggaaatagt ggatcttttg
       61 tataaagtaa accgattggg tactacggta gtggtggtta ctcatgatac tcgtatagtg
      121 gacagattac aacaaagagt aatacgtctg caggacggag aaatagtcag cgatacggaa
      181 ggaagtattt acgaaaatga agcctgtgag tattaaatac attttaatag agtcctttaa
      241 caacattgtc cggaacaagg tgttgtttat aacctctatc tttacggtgt gtgtgacgat
      301 actgctgatg ggtgttgtgg gaatacttct tatgaatgta tcctataact ccgataccat
      361 gatggaggaa atgcttaata taacggtgta tgtcataccg gaagcagatg aaaaaactct
      421 taacgatatt caggacgttt tagtagccga tgcacatgtg acggaaatta aatatgtctc
      481 acagatggat gcctatcagg cgggaataga acttgtaggt gaggaagtta tggcggaact
      541 ggggcatgaa tttcttcccg catcctttat tgtaaaactt gatgacgtaa catatgcgca
      601 tatattcgtg cgcaatatga aagcgatgtc caacatctat gaagtcaaat acagttccga
      661 agcatatgaa agagccggcg ccatatccta cacgataaga tatgtctgcg gggtgcttac
      721 tgcactgttg ggaattctat cgctgttcct tatatccaac accataaaga taactgtatc
      781 ggccagcaaa gaggaagtta ccattatgag atatataggg gcctcggaat cgagaataaa
      841 agctccgttc tatctgcagg gaggatttat agggttctta ggcgcaataa tttcttttat
      901 tatagccggt ttcggatata attatatgta tacaagatta gagggagact tgagcgaata
      961 tatgggtgtg ctggaactgg ttcccgttcc tcagatgctt cttgtattat tgggtgtatt
     1021 tttattgtac ggcattgttc ttggtatatt gggaagcttt tttgcgatac gcaagtatct
     1081 gaaagcttaa aagaggagta tgcaaatgat aaagggtact gaaaagttaa gaaaatgtgc
     1141 gattatactg gtctttgtgc tgttgctttc ttttactcag gcaacatttt gtgtagggga
     1201 cattaatgaa gccaaagata atctcgggaa catacaggac cagcttaaaa agattgaaaa
     1261 tgagattaaa caaaagcaga gcataaaatc atattacgag agccttgcca agaatatgaa
     1321 aaacagcatc agttctcaag agatacagct tgctacatat aacgagtata tagtggaggt
     1381 taccggtaag atagacagtc tgaccgaaac aatcgagcag gcggagcaag attatgccga
     1441 aagaataaag gtgttgaaag acagagttgt caatgcctat ataaacagca atctttccat
     1501 gctggatgtg ctgatgcagt cctcatcttt aaaagaatat tacgactata ttgaacttaa
     1561 taagcttatc accaaacacg ataatctgct tattgaagaa atgaaagtaa tgatgaagga
     1621 cataacggca aaaagagaaa ccatggagat gctcaaagct tcatatataa ctctaagcga
     1681 gcaggcacag atagtgctca gcaacatgca gtccatgcag agtgacgctt tagaaacaat
     1741 caatgtgacc ggcagtacca taaaggttct caaagcaaaa gaggatgctt tggaagccga
     1801 agaagcggaa atgcttaatc tcatattgaa gctgcaaaga gagatagaat atataggcgg
     1861 acagatgaca tggcctctgc cttcctggaa gcacattccc acgggtaacg gattgttcgg
     1921 ttggagactg catcccatat tcttcgaatg gagaatgcat aa
//
LOCUS       DAOPWC010000309         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_63892_length_1945_cov_3.707937, whole genome shotgun sequence.
ACCESSION   DAOPWC010000309 DAOPWC010000000
VERSION     DAOPWC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63892_length_1945_cov_3.707937"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKJ65_06530"
     CDS             complement(<1..276)
                     /locus_tag="PKJ65_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ41525.1"
                     /translation="MNDKIYYFDNAASTYMYDEVIEYINHINRNFYANASSTHLAGIA
                     CHDIVKADKEALCDILHINPNTLVFTSGGTESNNMAIKGTAFANFSRK"
     gene            complement(266..1924)
                     /locus_tag="PKJ65_06535"
     CDS             complement(266..1924)
                     /locus_tag="PKJ65_06535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ41526.1"
                     /translation="MKLTFYGAAREVTGSNFILDAAGKRVMIDCGLFQGSSATKAKNF
                     DGFKYDVQSVDYVLLTHAHIDHSGRIPLLYKIGYRGTVICTKATKDLCDIMLVDSANI
                     QESDTQYKNNKRFRAGLLPLEPLYTVEDANLALKHFIAVQYYDLIKLDDNITVKFSDA
                     GHMLGSASLTVFVTEEGKTTTVSFTSDLGKPDTPIINDPEVIEECDYLVMESTYGNKK
                     HILKEHPEERMFEIIEDTISNGGTVIIPSFSVGRTQEMIYAFNENIALKKKFSIFNKI
                     PVYVDSPLSTLATEVFKKNTQYFDDEAKQYILKGDNPLDFPNLIFTKSVEESKRLNFD
                     DSPKIIISSSGMCDAGRIRHHLKHNLYKEKTTVLFIGYQANGSLGRLLVDGAKSVKLL
                     GETIEVNARIEMIDGFSGHADMDDLDKYISQIKVKPEKIFLVHGEEDQLYPFAERIEK
                     TFGIETVIPENYYTYELKKGEKVYELDHDRVLTENTYKKLTLLDLVTDVKRQVKDVSE
                     LIAYDEINNLDDSQITVLIDLYAAMRQNIKDLIVGVESNTKVER"
BASE COUNT          547 a          398 c          333 g          667 t
ORIGIN      
        1 tttccgtgag aaattggcaa aagccgttcc cttaattgcc atgttattgg attccgttcc
       61 tcccgatgtg aatacaagag tattaggatt aatgtgcagt atgtcacata atgcttcttt
      121 atctgcctta acgatatcat ggcaagctat acccgcaaga tgggtactgg aagcatttgc
      181 ataaaagttc ctgtttatat gattaatata ctcaatcact tcatcataca tataagtcga
      241 tgcggcatta tcaaaataat aaatcttatc gttcaacttt tgtattactc tccactccta
      301 ctatcaaatc tttaatattt tgtctcattg ctgcatataa atcaatgagc accgttatct
      361 gactgtcatc gaggttgtta atctcatcat aagcgataag ctcggataca tccttaacct
      421 gtctctttac atcggtaaca agatccaata aggtaagctt tttgtaagta ttttccgtta
      481 ataccctgtc atgatccaat tcataaactt tttcgccttt tttgagttca taagtatagt
      541 aattttccgg aattaccgtt tctataccga aagttttttc tattctttcg gcaaagggat
      601 ataattgatc ttcttctccg tgaaccagga aaatcttttc cggtttgacc ttaatttgag
      661 agatatattt gtccaggtca tccatgtcgg catgaccgga aaatccgtct atcatctcaa
      721 tccgtgcatt aacctcaatt gtttcaccta aaagtttaac acttttggct ccgtcaacaa
      781 gcagtcttcc caaagagccg tttgcctgat atccgataaa aagcaccgtt gtcttttctt
      841 tatataagtt gtgtttaaga tgatggcgta ttcttccggc atcacacatt ccgctggagg
      901 aaataattat cttcggagaa tcatcaaagt tgagcctttt ggattcctcg accgatttgg
      961 tgaatatgag gttgggaaaa tccagaggat tatcgccttt caagatgtat tgtttggctt
     1021 catcatcaaa atattgagta ttttttttga agacctccgt tgccaatgtt gaaagcgggc
     1081 tgtccacata taccggaatc ttgttaaata tggaaaactt cttcttcaaa gcaatattct
     1141 cgttgaatgc ataaatcatc tcctgagttc ttcccacgga aaaagaaggt attattaccg
     1201 ttcctccatt ggatatggta tcctctataa tctcaaacat acgctcttcg ggatgttctt
     1261 tgagaatatg ctttttatta ccgtatgtgg attccattac aagataatca cattcttcaa
     1321 ttacttcagg atcatttatt atcggagtat cgggttttcc gagatcggaa gtaaacgaga
     1381 cagtagtggt tttaccctct tccgtcacaa aaacagttaa agatgcggag cctaacatgt
     1441 gtccggcgtc actgaatttt accgtaatgt tgtcatcaag ctttatcagg tcgtaatatt
     1501 gcacggctat aaagtgcttt aaagctaaat ttgcatcttc tacggtgtat aaaggttcca
     1561 gaggaagcaa accggctctg aatcttttat tgtttttata ctgtgtgtcc gactcctgta
     1621 tatttgcgga atccacaagc atgatatcac ataaatcttt agttgctttt gtgcagataa
     1681 ccgttcctct gtatcctatt ttatacaata aaggtattct tcccgagtga tctatatgtg
     1741 catgagtcaa aagaacataa tcaacagact gtacatcata tttaaaaccg tcaaagttct
     1801 tagccttggt tgcggaagat ccctggaata atccgcaatc tatcattact ctcttccctg
     1861 ccgcatccag tataaaattt gatcccgtta cttctcttgc tgcgccgtaa aatgttaatt
     1921 tcataataaa aaacctcttg ttgta
//
LOCUS       DAOPWC010000310         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_63913_length_1945_cov_2.700529, whole genome shotgun sequence.
ACCESSION   DAOPWC010000310 DAOPWC010000000
VERSION     DAOPWC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63913_length_1945_cov_2.700529"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..596
                     /locus_tag="PKJ65_06540"
     CDS             306..596
                     /locus_tag="PKJ65_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNZ41527.1"
                     /translation="MLHTVAFSLKHGKESDATNAFLSDGKKILTSIPVVRNFQVFRQT
                     SPKCTFDFGFSMVFTNRTDFETYNNHPDHVSFVKERWETEVSGFQETDYESL"
     gene            593..1825
                     /locus_tag="PKJ65_06545"
     CDS             593..1825
                     /locus_tag="PKJ65_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HNZ41528.1"
                     /translation="MSTLATPFARPLYMMVKPVGSVCNLACKYCYYLEKGNFYPEKKN
                     SIMTDELLEKFVQEYINSQTMSEVLFTWHGGEPLMRPISFYKKALEFQKIYGRGKQID
                     NCIQTNGTLLTDEWCQFFKENNFLVGISIDGPQEFHDEYRRNKQGQPSFYKVKKGIDL
                     LKKHGVEYNAMAVVNDYNVDYPLEFYNFFKEIECHYIQFSPIVERIHEQNNGTKLTHP
                     LQQNQEIKLAPFTVDSEKWGDFLCAIFDEWLKEDVGTYFVQLFDAALANWVGEQPGIC
                     TLAKTCGHAGIMEFNGDVYSCDHYVFPEYKLGNIRTHSLTEMMYSPKQLKFGADKADT
                     LPNQCKSCEYLFACNGECPKNRFLTTADGEPGLNYLCKGLKKYFTHIAPYMDFMKKEL
                     MAQRPPANVMEWARKQKQ"
BASE COUNT          623 a          375 c          419 g          528 t
ORIGIN      
        1 ctttattcat ttatcgctat ctgcttattt cgaaggtgca aatgtaggta tttttttatt
       61 ttattaatga agttaatccg gaatgggata aaagaaaaac tgaaataaat gggtaaaggc
      121 tgcccgttta ttccaaattc tattacattt gtaatctaaa cagttcaata atacatatat
      181 aatagtagtc tatgaacaga cgcagatttt tagaaacggc atcagtcgcc acagccgtgt
      241 cggctacagc gggagtgctt acttcttgta aagacgacgc cgcttgcaca ttaaaacccg
      301 gagaaatact tcatacagtt gcttttagcc taaagcatgg aaaagaatcg gatgctacaa
      361 atgcctttct gtccgacgga aagaagattc tgacatctat cccggttgtc agaaatttcc
      421 aggtattccg ccagaccagt cccaagtgca cattcgactt cggattttcg atggtcttta
      481 cgaaccgtac ggatttcgag acctacaaca atcatcccga tcatgtttca tttgtaaaag
      541 aaagatggga aactgaagtc agcggatttc aggaaaccga ttacgagagc ctatgagtac
      601 attagctacg ccctttgcgc gtccgcttta catgatggtg aaacctgtag ggtctgtatg
      661 taatctggct tgcaaatatt gctattacct tgaaaaagga aacttttatc cggaaaagaa
      721 aaacagtata atgactgatg agcttcttga gaaatttgtt caagagtaca tcaacagtca
      781 gactatgagc gaagtcttat ttacatggca cggaggtgaa ccactgatgc gtcctatcag
      841 cttttataaa aaggcgctgg agtttcagaa aatatacgga cgtggcaaac aaatagataa
      901 ttgtatccag acaaacggaa cgttgcttac ggatgaatgg tgccaattct ttaaggagaa
      961 taacttcctg gtagggatat caattgacgg tccacaggag ttccacgacg aatacagacg
     1021 taacaagcag ggacaaccct ccttttataa ggtaaagaag ggaatcgacc tgttaaaaaa
     1081 acacggagtc gaatataatg cgatggctgt ggttaacgac tacaatgttg actatcccct
     1141 cgagttttat aatttcttta aggaaataga atgtcattat attcagtttt cacccattgt
     1201 ggaacgtata catgaacaga acaacggtac taaacttact catccgctac aacagaatca
     1261 ggaaataaaa cttgcgcctt ttactgttga ttccgaaaaa tggggcgatt ttctctgcgc
     1321 tatattcgac gaatggttaa aggaggacgt gggaacctat ttcgttcagt tgtttgatgc
     1381 agccctggcc aactgggttg gagagcaacc cggtatatgt acgctggcaa aaacatgcgg
     1441 acatgccgga attatggaat ttaacggaga tgtttacagt tgcgaccatt atgtttttcc
     1501 ggaatataaa ctcggaaata tacgtacaca ctcgttaacg gaaatgatgt attcgccaaa
     1561 gcaacttaaa tttggcgccg ataaggcaga tacgctgccc aatcagtgta aatcgtgcga
     1621 ataccttttt gcctgcaacg gggaatgccc gaaaaaccgt tttcttacaa cagccgacgg
     1681 tgaaccggga ctaaactatt tgtgtaaagg tttaaaaaaa tacttcacac atattgcgcc
     1741 ttacatggat ttcatgaaaa aggaactcat ggctcaacgg cctccagcca atgtgatgga
     1801 atgggcgcgt aagcagaagc aataacacaa aaaagaaaaa gttatgagct ttaacagacg
     1861 ggaatttatt aaaacaggag gaattgttat gctgggatct ttagctactc cctcaatatt
     1921 aggtgaagtt gcagaaagtt atgcc
//
LOCUS       DAOPWC010000311         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_63915_length_1945_cov_2.662434, whole genome shotgun sequence.
ACCESSION   DAOPWC010000311 DAOPWC010000000
VERSION     DAOPWC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63915_length_1945_cov_2.662434"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKJ65_06550"
     CDS             complement(<1..702)
                     /locus_tag="PKJ65_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41529.1"
                     /translation="MDAFLGWFFEFMNYMLRGVIRFFVGIYEGIVQTFNVVKYIEIFR
                     SYYQQFKFLDWFFAILAIILITAVWLTVILLIILGIKRIINIRKNTVTQESIFEEITD
                     LHREILRLNDEKDRILSLKISQAGISAKDLAKELTDIAEKSREKVSQMDEFGMATTDS
                     TGDISVSSDQQQPEVMIESRFSKLIEVDQKYIYYTPQEYATGFSLEQLCKDFRNFACS
                     KMKLYYELEMMRILIS"
     gene            complement(733..1410)
                     /locus_tag="PKJ65_06555"
     CDS             complement(733..1410)
                     /locus_tag="PKJ65_06555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ41530.1"
                     /translation="MKKWLKTVGNVLLWVFIIFAVFMTVIAFTSTKNELGVSVILGKM
                     PITILTESMEPTLKKGDLIISHELKPDQKSLLKEDDIITYAVDLDKDGKMELNTHRII
                     SVRSSGGYVYYTTKGDNNAIADTDEVRYDNVIGVYNGRRVGGVGSVLNFLQTPNGFLI
                     CVVIPLVLFLLYELYNFIKVMMTVKNEKKNKLDEEEIKKKAIEEYIAQQNKEQEKSES
                     DADSAQS"
     gene            complement(1603..>1945)
                     /locus_tag="PKJ65_06560"
     CDS             complement(1603..>1945)
                     /locus_tag="PKJ65_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41531.1"
                     /translation="RFAILNSTASANIHTNKPIFAHNNESPIGALTTVETYSGTPTTS
                     SVSAVNTFTDANGLFTLAANGELDNSKKDPDNATVVTVRVWIEGQDADCITDNSNSAF
                     QVKFEFYVIEP"
BASE COUNT          569 a          417 c          326 g          633 t
ORIGIN      
        1 ggaaataagg atgcgcatca tttccaactc gtaatatagc ttcatcttgc tgcatgcaaa
       61 gttacggaaa tccttacaaa gctgttccag agagaatccg gtagcatact cttgcggagt
      121 ataatatata tatttctgat ccacttcgat aagcttggag aatctggatt caatcattac
      181 ttcgggctgt tgctgatcgg aagatacgga aatatcaccg gtagaatcgg tagtcgccat
      241 accgaattca tccatctggc tgaccttttc acgtgatttc tccgcaatgt cggtcaactc
      301 cttggccaga tctttagccg atatacccgc ctgtgatatc tttaaggata aaatcctgtc
      361 tttttcatca ttaagtctta gtatttctct gtgtaaatcc gttatttctt caaaaatact
      421 ttcctgtgtt acggtatttt tccttatgtt tataatcctc tttataccga ggatgataag
      481 cagtattacg gtaagccata ccgcagttat taagataata gccagaatgg caaagaacca
      541 atcgagaaac ttaaattgct gataataact tctgaaaatc tcgatatact ttactacatt
      601 aaatgtctgt actattcctt catatatgcc tacaaagaag cgtatcactc cccgtagcat
      661 gtagttcata aattcaaaaa accaacctaa aaacgcatcc atgcttaaat ctatccttta
      721 tttaagttta attcaggatt gtgcagaatc agcgtcgctc tccgacttct cctgttcttt
      781 attctgctgt gcaatatatt cttcaatagc ttttttctta atctcttctt catcaagttt
      841 attcttcttc tcattcttta ccgtcatcat aacttttatg aagttatata actcgtaaag
      901 taagaacagt actaaaggaa tgactacaca tatgaggaaa ccgttcggtg tctgcaggaa
      961 gttcaataca cttcctacac cgccaaccct tcttccgtta tatacaccta ttacgttgtc
     1021 gtaacgtacc tcatcggtat cggcaatagc attattgtca cctttggtgg tgtagtatac
     1081 atatcctcct gaactcctca cggaaataat cctgtgagtg ttaagttcca ttttgccgtc
     1141 tttgtccaaa tcaacggcat aagtgatgat atcgtcttcc tttaataagc tcttctggtc
     1201 aggcttcaac tcatggctga ttatgaggtc acctttcttc agtgtcggct ccattgactc
     1261 ggtcagtatg gtaatgggca tcttacctaa gataacagag accccaagtt cattcttggt
     1321 agaggtaaat gcaattactg tcataaatac agcaaatatt ataaacaccc ataaaagtac
     1381 attccctacc gtttttagcc actttttcat aatccgcctc cccgtttaac tatataatag
     1441 cacatctata tctagcagaa aaggtctttc aagcaaaagg aaagatcttt tccattaaac
     1501 aaatcagtgc aatcaactga aatgtttata ccgatttaga tatacaatcc cgcccgcagc
     1561 ccgtttaaag gctacgggca ggtaaatcag ttaaattaag agttacggct caattacata
     1621 gaattcgaat ttaacttgaa aagcggaatt ggaattgtct gttatacagt ctgcatcctg
     1681 tccttcaatc catactctta ctgtaacgac tgttgcatta tccggatcct ttttagagtt
     1741 atctaactca ccatttgctg caagcgtaaa aaggccgtta gcatctgtaa aggtattcac
     1801 agcagacaca cttgaagttg taggtgtacc gctataagtt tctactgtag ttaaagcacc
     1861 tatgggagat tcgttattgt gagcaaatat cggcttgtta gtatgtatat tagcagatgc
     1921 agtactgttc aaaatagcaa atctt
//
LOCUS       DAOPWC010000312         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_64205_length_1939_cov_3.511677, whole genome shotgun sequence.
ACCESSION   DAOPWC010000312 DAOPWC010000000
VERSION     DAOPWC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64205_length_1939_cov_3.511677"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKJ65_06565"
     CDS             <1..1224
                     /locus_tag="PKJ65_06565"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNZ41532.1"
                     /translation="PFEVQIRTWEMHRVAEFGIAAHWKYKQKIDDAEQSSSLEWISQW
                     MEWQKETRDEDEFYANLKNELFDLEVFVFTPKGDVINLPQGSNTMDFAYSVHSAVGNK
                     MVGAKVNGKMVPITYTLQTGDIVEIITSNTSKGPSRDWLKLVKTSQARTKIKQFFRKE
                     RREENIEKGKEQLYKEIRRLGLNPAELLKPQYYEKVLERYNFPELEDVMCAIGYGGIT
                     SNKIISRLKEEYKKHNKEEPKIDIQELPETVKTYKSSEGVIVRDIPNCLVRISKCCNP
                     VPGDDIVGYITRGRSVTVHRADCINVLNDKEFTERKIDVYWNMDSEDKYETQLEIKAY
                     DRANLVTDIMKLLSEQKIQCNGIKAKVASDGYADVHILVMIRNKDEISELNRKLRKIS
                     GIYEINRFSKRQKIR"
     gene            1221..1676
                     /gene="dtd"
                     /locus_tag="PKJ65_06570"
     CDS             1221..1676
                     /gene="dtd"
                     /locus_tag="PKJ65_06570"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ41533.1"
                     /translation="MIAVIQRVKESSVYINNEKYSEIKSGLNILLCVTGEDSEDDVYY
                     LADKIVKLRIFADEKDKMNLSLLDLNKELMVISQFTLAADTKKGNRPSFTYAADPEKG
                     NTLYELFISYIKEKYEITAKTGVFAANMQVHIVNDGPVTVIIDSRQNRK"
     gene            1748..>1939
                     /locus_tag="PKJ65_06575"
     CDS             1748..>1939
                     /locus_tag="PKJ65_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ41534.1"
                     /translation="MLSSNCYILISENEAAVIDPCVEPEMILNKINEVNINVKVEKII
                     YTHAHVDHIYYAEQLKEKTN"
BASE COUNT          742 a          283 c          433 g          481 t
ORIGIN      
        1 ccttttgaag tacagataag aacatgggaa atgcacagag ttgccgaatt cggtatcgcg
       61 gcgcactgga agtataagca aaagattgac gatgccgagc agtcatcttc attggaatgg
      121 atatcacagt ggatggaatg gcagaaagaa accagagacg aagatgagtt ttacgctaat
      181 cttaagaatg aactgtttga tctggaggta tttgttttca cacccaaagg cgatgtaata
      241 aatctgccgc aaggatccaa tacaatggat ttcgcataca gtgtacattc ggccgtaggt
      301 aataaaatgg tgggtgcaaa ggtaaacggt aagatggttc ccattacata taccttacag
      361 acaggggata ttgtggagat aattacatcc aatacttcaa aaggaccgag cagagattgg
      421 cttaagcttg taaagacaag tcaggcaaga accaagatta agcaattttt cagaaaagag
      481 cgaagagaag agaatataga aaaaggtaaa gagcagctgt ataaagaaat cagacgactc
      541 gggttaaatc ctgcggaact tctgaaaccg caatattacg agaaggttct ggaaaggtat
      601 aattttcccg agttggaaga tgtgatgtgt gcaataggtt acggcggcat tacatcaaat
      661 aaaataatat ccagacttaa agaagagtat aagaagcata ataaagaaga acccaagata
      721 gatattcagg aacttccgga aacggttaag acatataaga gttcggaagg tgttattgta
      781 agagatatac ccaattgttt agtaagaata agtaaatgct gtaatccggt accgggagat
      841 gacattgtag gatatataac aagaggaaga agtgtaacgg ttcacagggc tgattgtata
      901 aatgtgctca acgacaagga atttaccgaa agaaaaatag acgtatactg gaatatggat
      961 tccgaagata aatatgaaac acaattggaa atcaaagctt atgacagagc taatctggtt
     1021 acggatataa tgaaactctt gtccgagcag aagatacagt gtaacggaat aaaggcaaag
     1081 gtagcctcag acggatatgc cgatgttcat atactggtta tgataagaaa taaggacgag
     1141 atttccgaac ttaacagaaa gctgagaaag atatcgggaa tatatgaaat aaacagattt
     1201 tccaaaaggc agaagattag atgatagcgg ttattcaaag agtaaaagaa agttcggtat
     1261 atataaataa cgaaaaatat tcggaaatta aatcaggctt aaacatatta ctttgtgtta
     1321 ccggggaaga cagtgaggac gatgtatatt atcttgccga taaaatagta aaacttcgaa
     1381 tatttgcgga cgagaaggat aagatgaacc tgtctcttct ggacttaaat aaggagctta
     1441 tggtaataag ccaattcact ctggcggcgg ataccaaaaa aggtaacaga ccttctttta
     1501 cctatgccgc ggacccggaa aaaggtaaca cattatatga actttttata agttacatta
     1561 aagaaaagta cgaaattacc gctaaaaccg gagtatttgc cgcaaacatg caggttcata
     1621 tagttaatga cggaccggta accgttatta tagacagcag gcaaaacaga aaatagagta
     1681 tttttttgaa gattgaggag aaaataaaat aaacagtgaa tttaaaatat taagaataac
     1741 cagtcctatg ctttcatcaa attgctatat tctgatatcg gaaaatgaag cggcagttat
     1801 agacccttgt gtggaacctg agatgatatt aaataagata aatgaggtta atattaatgt
     1861 taaagttgaa aagataatat atactcatgc ccatgtcgac catatctatt acgcggaaca
     1921 gttgaaagaa aagacaaat
//
LOCUS       DAOPWC010000313         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_64464_length_1934_cov_3.017030, whole genome shotgun sequence.
ACCESSION   DAOPWC010000313 DAOPWC010000000
VERSION     DAOPWC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64464_length_1934_cov_3.017030"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKJ65_06580"
     CDS             <1..742
                     /locus_tag="PKJ65_06580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ41535.1"
                     /translation="TSWRDTAKWVMATVSDKEEILLTALMTPPHNITLYKTDNVMCSE
                     ALDCLIAGLGDIYVPGVISEKQLATAFADKYSSLKGMTYYTQMNQRIYELEKVNPGIE
                     RIGTIRLVNNNDMHFFPYWLSAFYASNVYGQTVMNIPESEREAQYRIDMKKIYILEVN
                     GIPVSMAGYNRELPASIGVAYVYTPPYFRNKGYASSAVAQVSQLALDRGYKRCVLYTD
                     LSNPVSNSIYGKIGYVPVCDSLMLQFNN"
     gene            765..1229
                     /locus_tag="PKJ65_06585"
     CDS             765..1229
                     /locus_tag="PKJ65_06585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNZ41536.1"
                     /translation="MCLICDRIKMIKDGTDKYLVRELETGYVVLGDHQHISGYTLFLC
                     KEHKCELFELEHSFMVKYMVEMALVAEAISKAFKPEKINYQLTGNGETHLHWHLFPGV
                     SGDIGDYGDNGKGPVWCYPMEKMYSDENKLSDQQPEEMKTKLQKQLDKLLKV"
     gene            1340..1660
                     /locus_tag="PKJ65_06590"
     CDS             1340..1660
                     /locus_tag="PKJ65_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41537.1"
                     /translation="MKRRIIKGIILSVLCLVLLASLTSCAMGLSGTYKKGSDLAYETY
                     RFSGNKFYYEVGALGVKVGGHEGTYKIKDGKITFIYDDSEHTYKFEKGKDYIIINGAS
                     YDKK"
BASE COUNT          647 a          323 c          419 g          545 t
ORIGIN      
        1 gacaagctgg cgggatactg ccaaatgggt tatggcaacc gtatccgata aggaagaaat
       61 tcttttgaca gcacttatga cacctcctca taacattacc ctttataaaa cggataatgt
      121 tatgtgctct gaagcattgg actgcctgat tgcaggattg ggtgatatat atgtaccggg
      181 agttatttcg gaaaagcagt tggcaacggc atttgcggat aaatattctt cgttaaaggg
      241 aatgacatat tatactcaga tgaaccaaag aatatatgaa ttggagaaag taaatcccgg
      301 tatagaaaga attggaacaa tacgtcttgt gaataataac gatatgcatt tctttcccta
      361 ttggctgtct gctttctatg catccaatgt gtacggacaa acggtaatga atatacccga
      421 gagcgaaaga gaagcacaat accggataga tatgaaaaaa atatacatac tggaagtaaa
      481 cggaataccc gtttccatgg cgggatataa cagggaactg ccggcatcca taggtgtggc
      541 ttatgtttat acaccaccgt atttcaggaa taaaggatat gcaagttccg ctgtggctca
      601 ggtcagtcag ctggctctgg acagagggta taaaaggtgt gtattgtata ccgatttgtc
      661 caatcccgta tccaacagta tttacggtaa gattgggtat gtccctgtat gcgactcatt
      721 aatgctgcaa ttcaataatt aatatatatt acggaggaag aaaaatgtgt cttatttgcg
      781 acagaattaa aatgattaag gacgggaccg ataaatattt ggtcagggaa ctggaaacgg
      841 gatatgttgt attgggagat catcagcaca tatccggcta taccctgttt ctttgtaaag
      901 agcacaagtg tgaattgttt gaactggaac attcatttat ggtgaaatac atggtggaaa
      961 tggctcttgt tgccgaagct atatccaaag ccttcaaacc ggaaaagata aattatcaat
     1021 taacaggtaa cggagaaacc catctgcact ggcatttatt tcccggagtc agcggtgata
     1081 taggggacta tggggataac ggaaaaggac cggtttggtg ctatcctatg gaaaaaatgt
     1141 acagtgatga gaataaactt tccgatcaac aaccggaaga gatgaaaaca aaattacaaa
     1201 agcaattgga taaactgctt aaggtttaga taaccgacaa atatcttcaa agtgttctat
     1261 aactgaaaat tgcattgatt atacttgcag taatggtata ataactttgt caatttttta
     1321 tcactaatag gaggaaagaa tgaaaagaag aataataaag ggaattatac tgtccgtact
     1381 gtgtctggta ttgcttgcat ccctgacatc gtgtgcaatg ggacttagcg gaacatacaa
     1441 aaaaggttcc gatctggcat acgaaaccta cagattcagc ggtaataaat tttactatga
     1501 agtgggtgca ctgggtgtaa aagtaggcgg tcacgaaggc acatataaga taaaagacgg
     1561 gaagattact tttatctatg acgacagtga acatacttat aaatttgaaa aaggcaaaga
     1621 ctatataata atcaacggcg cttcctatga caagaagtag ggaagaggag taatttattt
     1681 aagcagatat aaattgaatt tgttaacaaa aaaagagctt gtatgggctc ttttttgcat
     1741 gcagattagt cttaaaatct tcttctgcaa tctccatttc ctcaggaatc tgtccgattg
     1801 tcggcaagtg ctttccgata caatttgccg cgtaagtgcg tagccttttt ctccttgtat
     1861 aacttaaatc ttataattac ttacccatta acttacccat agcatatcaa atggacagtt
     1921 aaatggcaat actt
//
LOCUS       DAOPWC010000314         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_64882_length_1926_cov_3.365580, whole genome shotgun sequence.
ACCESSION   DAOPWC010000314 DAOPWC010000000
VERSION     DAOPWC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64882_length_1926_cov_3.365580"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKJ65_06595"
     CDS             complement(<1..736)
                     /locus_tag="PKJ65_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41538.1"
                     /translation="MSGIMLNWDDSAFFFGIHKGYGESVKDKQEAIAYTEAVMGQYQG
                     TNVTDIVMCVNARLASYPSVTIENYGDSYRRKVQNGEQVDFKKTWVSAYDKIFNQWGI
                     DHFQIWIDKLKKMNIKPWISIRMNDAHETMKKTSCIAPDFYYDNPQYRRVTYSPVQLY
                     YDGLYDYGQAAIRKRFLDFIDETLGRYDVPGLELDWMREAYLFRYGMEQEGITILTGF
                     MKDVKKIVKKYEKKRGHDISISVRVPS"
     gene            988..1731
                     /locus_tag="PKJ65_06600"
     CDS             988..1731
                     /locus_tag="PKJ65_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HNZ41539.1"
                     /translation="MEKKGLNANHLKLIAIIAMTADHIADLFYPGFPVKPLPIILHLI
                     GRLTAPIMWFFVCEGFYYTRNVKKYMLRMFIFAVISHFAYCFSFGIDPVPFRSGIFNQ
                     TSVMYPLFISVVVLWLEHEEKRMNKYLKHIILFLLICSAFPADWSCIAVLAIISMYKK
                     RGDLKGQMKMMLLWVFFYGLISFFFVSKVYALELIGVLMVYPVLKMYNGQKGKAGWMK
                     MFFYIYYPLHLVVIGIIRILMYGDVPLLF"
BASE COUNT          550 a          387 c          340 g          649 t
ORIGIN      
        1 cgctgggtac tcttacggat atggatatat catgacctcg ttttttctca tattttttga
       61 caatcttttt tacatccttc ataaaaccgg tcagtatggt aataccttcc tgttccatac
      121 cgtatctgaa caggtaagct tccctcatcc agtccagttc cagtccgggc acatcataac
      181 gacccagagt ttcatcaata aaatctaaaa accgctttct tatcgccgcc tgtccgtaat
      241 cataaagacc gtcatagtat aactgaaccg gactgtatgt cactcttctg tattgcggat
      301 tgtcataata gaaatccggt gcaatacagg atgttttctt catggtctca tgagcatcat
      361 tcatccttat tgatatccag ggtttgatat tcatcttttt aagtttatct atccatatct
      421 gaaaatgatc tattccccat tggttaaata ttttatcgta agccgatacc catgtctttt
      481 tgaaatccac ctgctcaccg ttctgaactt ttcttctata tgaatcgccg taattttcaa
      541 tagtcactga cgggtaagat gccagtctcg cattaacgca cattactata tccgttacat
      601 tggtcccctg atactgaccc attacagctt ccgtatatgc gattgcttcc tgtttatctt
      661 taacactttc cccgtatcct ttgtgaattc cgaagaagaa tgcactgtca tcccaattca
      721 acattatacc gctcatatca tccctcctat acagtcgatt ataccatgaa taccttacgg
      781 aataattgaa aacaacttat ttattcgtaa aaaacatacc ttacgcttat atgtattgat
      841 aatatgcttt atgaataaga actgagagat atatccgtta ccgggccaag taaaatatgt
      901 ttatgtcaat tttatattta tttgacataa atattacctg aatccactat aattaattta
      961 ccactcccgc gtgagggcga ggaaaaaatg gagaaaaagg gacttaatgc aaatcatctg
     1021 aagttaatag caattattgc gatgacagca gaccatatcg cggatctttt ttatccgggc
     1081 tttcctgtga agcccttgcc tatcatactt catctgatag gcaggctgac agctcctatc
     1141 atgtggtttt ttgtctgtga gggtttttac tatacaagaa atgtgaaaaa atacatgctc
     1201 agaatgttca tatttgcggt catatcgcat tttgcatatt gcttttcatt cggcattgat
     1261 ccggtgcctt tccgttcggg aatttttaat cagacaagtg ttatgtaccc gctgtttatt
     1321 tccgttgtag tgctgtggtt ggagcatgaa gaaaagcgta tgaacaaata tctgaaacac
     1381 ataatacttt ttctgctcat atgcagtgca ttccccgctg actggagctg cattgcggtg
     1441 ttggcaatca tcagtatgta taaaaagcgc ggagatttaa aaggacagat gaaaatgatg
     1501 cttctgtggg tgtttttcta cggattgata tcgttctttt ttgtcagcaa agtgtatgca
     1561 cttgagctaa tcggtgttct catggtttat ccggtgttaa aaatgtataa cggtcaaaaa
     1621 ggaaaagccg gctggatgaa aatgtttttc tacatttatt atcctctgca tttagtcgtg
     1681 ataggaataa tacgcatatt gatgtacgga gatgtaccgt tactgtttta gtttgatacc
     1741 cttgcttttt aatttaccaa catattactg catttacact tgtaaatcct gaagaaataa
     1801 ctaaacttat attatgctct ccggttttta gagcaaaaca aaaaaatgtc ctcacggact
     1861 gaagaaagaa tatgaaatac agacttatcg caacggatat agacggtaca cttttggatg
     1921 atttcg
//
LOCUS       DAOPWC010000315         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_65170_length_1920_cov_2.302413, whole genome shotgun sequence.
ACCESSION   DAOPWC010000315 DAOPWC010000000
VERSION     DAOPWC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65170_length_1920_cov_2.302413"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKJ65_06605"
     CDS             complement(<1..916)
                     /locus_tag="PKJ65_06605"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNZ41540.1"
                     /translation="MRYTGVTSRGIITPIFKQGDDLVSMIADSVIKAAEKENFSIQDR
                     DIIGVTEAVLARTQGNYATCEQIAADIRSKFGGKDLGIVFPILSRNRFLLLLKAIAMS
                     CEKLYVQLSYPADEVGNSLITLDDLDETQVDPYTDSFTEVEFRKLFKNTAHIFTGVDY
                     IEVYKNIGKHVEIIFSNNPSYILNYTKNVLNCDIHSRKRTQKILRKAGAEKVYRLDEI
                     LTSSVQGSGYNEEYGLLGSNNATGDSVKLFPRDAQIFVEQLQKRVSDLTGKHMEVMVY
                     GDGCFKDPVGGIWEFADPVVSPGFTSGLL"
     gene            complement(1025..>1920)
                     /locus_tag="PKJ65_06610"
     CDS             complement(1025..>1920)
                     /locus_tag="PKJ65_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41541.1"
                     /translation="TAVNMLRTLKAGHNICMFPEGSCTWNGETATIIQSTAKLIKRAG
                     VAMITYRVEGGYPSMPRWSKSKRPGKMIGKIGNIYEPDVLEKMTVEEIYQAMCKDLYV
                     NSIDMTEKYEYKGKTLAENLETVLFVCPKCQGISTYTSKDDIFSCKCGVRLRYDTHCR
                     LNDENNEGFPYTSVLEWDKWQRQWVIDNIERMKKNPDKVITEDDNQELFTYTEDEKTE
                     HVLSGSMALYPDKFVFTDKVNKKELVINLSDITDIAIITMTTLTFSCSDLHYEIKCKI
                     PRSALKYLLIATGLSPMRYII"
BASE COUNT          512 a          454 c          320 g          634 t
ORIGIN      
        1 ccagaagtcc cgaagtgaaa ccgggagaca ctaccggatc ggcaaactcc catatacctc
       61 ctacaggatc tttaaagcaa ccgtcgccgt aaaccattac ctccatatgc tttccggtta
      121 aatccgacac tcttttttgc agttgttcca caaaaatctg agcatccctc ggaaaaagct
      181 ttacactgtc tcccgtagca ttattggacc ctaacagtcc gtattcctca ttgtagcccg
      241 atccctgtac actcgatgtc agtatctcat ctaatctgta caccttttcc gctccggctt
      301 ttcttaatat tttctgcgtg cgttttcttg aatgaatatc acaattcaga acatttttcg
      361 tatagttcag aatatatgag ggattgttgg aaaatattat ttccacatgc ttacctatgt
      421 tcttgtacac ttcaatgtag tccactccgg taaatatatg agcggtattc ttaaacagtt
      481 ttctgaactc gacctcggta aaactgtctg tataagggtc aacttgtgtt tcgtccagat
      541 cgtcaagggt aataagggaa ttacctactt catcagccgg ataggacagt tgcacataca
      601 gtttctcaca ggacatggca atagccttga gtaataacaa aaacctgtta cgactcaaaa
      661 taggaaacac tattcctaag tctttaccgc cgaacttgct ccttatatct gccgcaatct
      721 gctcacaggt tgcataattg ccctgtgttc ttgccaaaac agcttcggtt actcctataa
      781 tatccctatc ctgaatggaa aaattctctt tctccgcagc ttttattaca ctgtccgcaa
      841 tcatcgatac caaatcgtct ccctgcttaa atatgggggt aataattcct ctggatgtaa
      901 cacctgtata cctcatttat agcacttcct tccctcaaag tgtccgcatg gacaacacac
      961 tgaattttaa aatatacata tataaatgtc aaatagcttc ttgttttaat gcgttaaaaa
     1021 accgctatat tatatacctc atgggtgaca gaccggttgc gataagaagg tatttcaatg
     1081 ccgatctcgg tattttgcat ttgatttcat aatgcaaatc cgagcaggaa aaagtcagtg
     1141 ttgtcattgt aattatggca atatccgtaa tatccgagag atttataacc agctcctttt
     1201 tgtttacttt atcggtaaac acaaatttat ccggatacag agccattgaa ccgctcagaa
     1261 catgctcggt cttctcgtct tcggtatatg tgaacagctc ctgattgtca tcctctgtta
     1321 ttactttatc gggattcttc ttcattcttt caatattgtc tatgacccac tgtctctgcc
     1381 atttgtccca ttccagaacg cttgtatacg gaaatccctc attattttcg tcattcagtc
     1441 tgcagtgagt atcgtatcgc agtcttacac cgcatttaca ggaaaatata tcgtcttttg
     1501 atgtatatgt gcttatacct tgacatttag ggcaaacaaa cagtaccgtt tccagatttt
     1561 ccgcaagtgt tttccctttg tactcatatt tttccgtcat atcaatggag ttaacatata
     1621 agtctttaca cattgcttga tatatttcct ccactgtcat cttttccaat acatcgggct
     1681 cataaatatt acctattttg ccaatcatct tacccggtct tttggatttt gaccatctgg
     1741 gcattgaggg ataaccgcct tccacacgat atgttatcat cgcaacacct gctcttttaa
     1801 taagttttgc ggtagactgt ataatggttg ccgtttcgcc gttccatgta caactgcctt
     1861 cgggaaacat gcagatatta tgaccggctt ttaaagttct gagcatatta accgccgtct
//
LOCUS       DAOPWC010000316         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_65427_length_1915_cov_2.726344, whole genome shotgun sequence.
ACCESSION   DAOPWC010000316 DAOPWC010000000
VERSION     DAOPWC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65427_length_1915_cov_2.726344"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PKJ65_06615"
     CDS             complement(<1..1705)
                     /locus_tag="PKJ65_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ41542.1"
                     /translation="MERFTSGIVKRRKLIMIIFITLAVICGIMAGGVLINYNLEDYLP
                     KDSNSTEGMSLMEKEFDEDFPTASVMVRNVSVHQALEYKENLENISGVTAVTWLDDVI
                     GQDVLASTPLEFLDSSVIEAYYKNGNALFSVSIAPGFEVEAVDGIYEVIGEENAASGS
                     SVSKAVVQKLSATETLRSLLILVPLIIIVLLLTTSSYIEPLLYLAALGVAILINMGTN
                     LLFDDISFITYTVSPILQMAVSLDYAIFLMHSFKEYRERYEPEEAMKRAIKKSFTAVA
                     ASAATTVIGFLALIFMRFGIGADLGINLVKGVVLSFISVMIFLPALALSFVRIMDKTQ
                     HKTVRMDLKRVGQWLMKLRIPLLIISILVIIPCFLAKDKITFEYGSGAGIKASRAEED
                     KLAIEEQFGKINQMAILVPKGGNGSLNMLSNRLEEIEGISAVISYASYIGPAIPEGFV
                     EGQDSMFNSENYSRIILYTDYEIESEETFAAIEKIRATAEQYFDEVYLVGESASVYDI
                     KDSITADNTLVTLIAVLGIFLVILISFKSLSLPFLLVFAIEAAIWINLSFTYFQGGSL
                     NY"
BASE COUNT          566 a          434 c          372 g          543 t
ORIGIN      
        1 tatagtttaa agagccgcct tggaaatatg tgaatgaaag gtttatccat atcgccgcct
       61 ctatggcaaa gaccaataaa aagggaaggg ataaagattt gaaagaaata agtattacaa
      121 ggaatatccc caataccgca atgagtgtaa caagggtatt gtcagccgtt attgaatcct
      181 ttatgtcata caccgatgca ctttctccca ctaagtagac ctcgtcgaaa tactgctctg
      241 cggtagcgcg aatcttctct atggctgcaa aagtttcttc actttctatt tcatagtcgg
      301 tatacagtat tattctggaa tagttctccg aattgaacat actgtcctgt ccttccacaa
      361 agccttccgg aattgccgga cctatatagc ttgcgtagga tatgacagcg gaaattccct
      421 ctatctcttc cagtcggtta ctcagcatat tcagggaacc gttaccgcct ttgggtacca
      481 gtatggccat ctgattaatc tttccgaatt gctcttctat agccaatttg tcctcctccg
      541 ctctggatgc ctttataccg gcaccggagc cgtactcgaa cgtgatttta tcctttgcaa
      601 ggaaacaggg gataataacc agaatggata ttattaaaag aggtattctc agcttcatca
      661 gccactgtcc caccctcttc aaatccatac gtacggtttt gtgttgtgtt ttatccatta
      721 tacgtacgaa ggaaagagca agtgccggca ggaatatcat tacgcttata aagctcagta
      781 ccactccctt aaccaggttg atacccagat ccgctcctat tccgaatcgc atgaatatta
      841 aagctaagaa tcctataacc gtagtggctg cactggcagc taccgcggta aaggatttct
      901 ttatcgcacg cttcatggct tcttccggtt cataccgttc ccggtattct ttaaagctgt
      961 gcattaagaa tattgcatag tccagtgaga ccgccatctg caaaataggg ctgacggtat
     1021 aagtaataaa ggaaatgtcg tcaaataaca gattggtgcc catgttaatc aatattgcga
     1081 cacccaatgc cgcaagatac aacagaggct ctatatatga cgatgtggtt aaaagtaaaa
     1141 caatgataat gagaggaacc agtatgagca gcgaccttaa tgtttcggta gccgaaagtt
     1201 tctgtacaac cgctttactg accgaagagc ccgatgcggc attttcttca cctattacct
     1261 catatatacc gtctaccgct tccacctcga aaccgggcgc tatggaaacg gaaaacaggg
     1321 cgttgccgtt tttgtaatat gcttcaatca ccgagctgtc cagaaactcc aaaggcgtgg
     1381 aagcaagcac atcctgtcct atcacatcgt ccagccatgt gacagctgtg acaccggata
     1441 tattttccag attctcttta tactccaatg cctgatgaac cgatacattc cttaccataa
     1501 cacttgcagt agggaagtcc tcatcaaatt ctttttccat taaactcata ccttcggtgg
     1561 aattactgtc cttgggaaga taatcttcca gattatagtt gataagaaca ccgccggcca
     1621 ttattccgca tatcaccgca agagtaatga atataatcat tatgagtttt cttcttttaa
     1681 caataccgct tgtgaatctt tccataacac tccttattat aaacaacatt gtgttgacaa
     1741 atcaccactg ttataggata atatcaatgg gttgataagt caacagacag atgtttacca
     1801 aatgtttgtt ggcaaacaca aaagagggaa atgtggttta tttgacaaaa acgggggaga
     1861 tgacaatgaa gaagcaaaaa aatgaatgtt ggtaaatcct atctgctcca attgc
//
LOCUS       DAOPWC010000317         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_65698_length_1910_cov_2.580054, whole genome shotgun sequence.
ACCESSION   DAOPWC010000317 DAOPWC010000000
VERSION     DAOPWC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65698_length_1910_cov_2.580054"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKJ65_06620"
     CDS             complement(<1..1071)
                     /locus_tag="PKJ65_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ41543.1"
                     /translation="MVKSRRSNRSDKNNELLRNDIKNNGYYTETIKTQKKSFVSRIIL
                     PLFICILCITLSVCATALTVFYVRKINQPDTDLLFINNPIQSPEPVNTENPVPTETQE
                     VDTDTRPDEIRENLVITTYRDISRVEVTPLEVDSTISDAVQKIMPSIAYLKCMYADEK
                     GVHMTEASALIISNDGYMITANSVIEPLCYDKSNEFKENCSISVFVDFDFNILYSAKV
                     IGRDKTKNVALLKIDCDRHLQFIEYGDSDKLYLGETVIAVASGNNAFKGQVSAGIVTG
                     LSYTFHDRILSDSETPKDILYMINTSAVMTSTNNGGALVNKKGQVVALTVFMKDEQQN
                     GFYKAIPIYKVKEASENLEVKPY"
     gene            complement(1123..1659)
                     /locus_tag="PKJ65_06625"
     CDS             complement(1123..1659)
                     /locus_tag="PKJ65_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ41544.1"
                     /translation="MVRDLLVLTKLDAEEDDSLVKNRFDINDMIRKSIIRFEEEITEK
                     KLFINVNFYAEVTYVYANSDDIERVLINLIHNAVKFTNDGGNITITVEPKRGKVHVSI
                     RDNGIGIEQEDLENIWTRFYKSDKSRGRDGTGTGLGLPIVFSVIKRHKETIDVKSRRG
                     IGTEFIFTLENAEQTDNS"
     gene            complement(1679..>1910)
                     /locus_tag="PKJ65_06630"
     CDS             complement(1679..>1910)
                     /locus_tag="PKJ65_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ41545.1"
                     /translation="VRAKYTDDRTELGELSKTFNSMMERLEKTEQQRAEFTSNVAHEL
                     RTPMTSIGGFVEGILDGTIPEEKHITYLKSFR"
BASE COUNT          507 a          418 c          316 g          669 t
ORIGIN      
        1 atacggtttg acttccaggt tttccgaggc ttcttttact ttgtaaatag gaatagcttt
       61 ataaaaaccg ttttgttgtt catctttcat aaaaacggtc aatgcaacaa cctgaccctt
      121 tttattaacc aatgcgccac cgttattagt gctagtcata acagctgaag tgtttatcat
      181 gtacaaaata tccttcggtg tttcagagtc ggataatatc ctgtcatgga atgtataaga
      241 aagaccggtt acaatacctg cggaaacctg ccccttgaac gcattattgc cgctggcaac
      301 tgcaataacg gtctcaccga gatacagttt gtcggaatca ccgtactcta taaactgtag
      361 gtgtctgtca caatctattt ttaaaagagc tacatttttt gttttatctc tgcctatcac
      421 cttcgcggaa tagagaatat tgaaatcaaa atccacaaat accgagatag aacagttctc
      481 tttaaattca ttacttttat catagcacag aggttctata acactgtttg cggttatcat
      541 atatccgtca ttggaaataa tcaaagcgga ggcctcggtc atgtgaacac ccttttcatc
      601 agcatacatg catttgagat aagcaattga cggcattatc ttctgtacgg catcactgat
      661 tgtggaatcc acttccagcg gagttacctc cactctggat atatccctgt atgtggttat
      721 aaccagattc tctcttattt catccggtcg ggtatcggta tcaacctctt gcgtttccgt
      781 aggcaccgga ttttcggtat taacaggttc gggactttgt atcgggttgt ttataaaaag
      841 caaatcggta tcgggctgat ttatctttct gacatagaat accgttaaag cagttgcaca
      901 taccgataat gtaatacaca atatgcatat gaacagcgga agaattattc tggaaacaaa
      961 actctttttc tgagttttta tggtctcggt ataataaccg ttattcttta tatcgtttcg
     1021 taataattcg ttattcttgt cacttcggtt acttcttctt gactttacca ttttgccctc
     1081 atagtgatag taccacactt taatgtttta tttgttaact ttttatgaat tatccgtttg
     1141 ctctgcattt tccagtgtaa atataaactc ggtacctata cctcttctgc tcttaacatc
     1201 aattgtttcc ttatgtcttt taataacact gaacacaata ggcagtccca accccgtgcc
     1261 ggttccgtct ctacctcttg atttatcgct tttataaaat cttgtccaga tattctccag
     1321 atcctcctgc tcaataccta ttccgttatc tcttattgaa acatgcacct tacctctttt
     1381 gggttctaca gtaatagtaa tattacctcc gtcgttggta aattttacgg cattgtgtat
     1441 taaattgata agtaccctct caatatcatc ggagtttgcg taaacatatg tcacttctgc
     1501 ataaaagtta acatttataa acaatttttt ttccgttatc tcttcttcaa agcgaatgat
     1561 ggactttctt atcatatcgt taatgtcaaa ccgatttttt accaaagaat catcctcttc
     1621 ggcatccaat ttagtcagaa ccagcagatc ccgtaccata ttggtaagac gccttatttc
     1681 atcggaaact cttaagatag gttatatgct tttcctcggg gatggttccg tctaaaatac
     1741 cttccacaaa tcctcctatt gatgtcatgg gggttctcag ttcatgagcg acattactgg
     1801 taaattccgc tctctgttgc tcggtcttct ccagtctttc catcatgctg ttaaaagttt
     1861 tggataattc ccccaattcg gtcctgtcat cggtatactt tgcacgcact
//
LOCUS       DAOPWC010000318         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_66079_length_1903_cov_2.393398, whole genome shotgun sequence.
ACCESSION   DAOPWC010000318 DAOPWC010000000
VERSION     DAOPWC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66079_length_1903_cov_2.393398"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PKJ65_06635"
     CDS             complement(<1..1169)
                     /locus_tag="PKJ65_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41546.1"
                     /translation="MKYTLDLYKASLKSYEIYLERLIRNQMTQASDWRKDGVIIVDEG
                     FVPTHIAGVLYAPGLLYFNEDSKYYKSKKAMEFAFSVLEFFERNLSPEGLMDFYMCNF
                     RSAPDTSFSIQPLVDLYNIIKDKKTDETGELFKKRLYGFIVKIADGVFGGGFHTPNHR
                     WVITSALAMVMNMTGDKKYLKRINEYLAEGIDCDEEGEYSERSSGGYNFVNNEAILII
                     AQELDKPELLEFVRRNLMMMTYYIHTDFLIFTQNSTRQDKGKENIYLDKYLYQYLKAG
                     YLLKDEQLLGVAKAIQNELFSKRRGYPIGLDTMMAEPEVLIIPEHIKPFEIQPYVKHF
                     KKSNIVRVYHNGMTLSVIEDRETFLYLKYKSFDMFIQGGILFFNERHIKVKNIRP"
     gene            complement(1290..1559)
                     /locus_tag="PKJ65_06640"
     CDS             complement(1290..1559)
                     /locus_tag="PKJ65_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ41547.1"
                     /translation="MTYSIEVTSRFEREFKKLDRYTKLMINAWIEKNLLDCENLGTCK
                     KTLIENRKGLWRCRIGDYRLICHIEDKKFIILALSIGHRSEVYKS"
     gene            complement(1556..1777)
                     /locus_tag="PKJ65_06645"
     CDS             complement(1556..1777)
                     /locus_tag="PKJ65_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HNZ41548.1"
                     /translation="MAFSIRLTEEERALAESYAKLHSYTLAEAFKRALFEKIEDQYDI
                     AIYDAAYKEYEDSGKQSRPIKEFWKELQT"
BASE COUNT          533 a          389 c          317 g          664 t
ORIGIN      
        1 atgggacgga tatttttaac tttaatatgt cgctcgttaa aaaagagtat tcctccctga
       61 ataaacatgt cgaaactctt atatttaaga tacaaaaagg tttctctgtc ttcaataacc
      121 gataaagtca taccgttgtg ataaactctt actatgttgg attttttgaa atgctttaca
      181 tacggctgaa tttcaaacgg ctttatatgt tccggtatta tcaatacctc cggttccgcc
      241 atcatggtgt ccaatcctat agggtatcct cttcttttgg agaaaagttc attctgtatg
      301 gcttttgcca caccgagcaa ctgctcatcc ttaagaagat aacccgcttt aagatactgg
      361 tagagatatt tatccagata tatgttttct tttcctttat cctgacgggt ggaattctgc
      421 gtgaatatga gaaagtcggt atgtatgtag taggtcatca tcataagatt cctgcgcacg
      481 aattccagca gctccggttt atcaagttcc tgtgctatta ttaaaattgc ttcattattg
      541 acaaaattat atccgcccga agagcgctca gagtattctc cttcctcatc gcaatcgata
      601 ccttcggcca gatattcatt tattctctta agatattttt tatctccggt catgttcatg
      661 accatagcca gagccgatgt tataacccat ctgtggttgg gggtatggaa tcctcctcca
      721 aaaactccgt ccgctatttt tactatgaaa ccgtaaagtc ttttcttaaa gagttctccc
      781 gtttcatcgg tttttttatc tttaatgata ttgtaaagat cgaccagagg ctgtattgag
      841 aaggatgtat ccggtgccga ccggaaattg cacatataga aatccataag tccttcgggg
      901 cttaagtttc tttcaaagaa ctccagaacg gaaaaggcga actccattgc tttttttgat
      961 ttatagtatt tactgtcctc attgaaatac agtaatccgg gagcatataa aacccctgcg
     1021 atatgagtgg gcacaaagcc ctcatccaca ataatgacac cgtcttttct ccagtcggat
     1081 gcctgtgtca tctgatttct tataagtctt tccagataaa tttcatatga ttttaaactt
     1141 gctttgtaca gatccaatgt atatttcatt acatctctcc ttgccctttt actcattata
     1201 gccgatgggc aattttaagc aacatcaaat cataagttat cgggatattg gataagaaac
     1261 tgttttagaa aaaacatgaa aatgagatat cagcttttat aaacttcact gcgatgtccg
     1321 atgctcaacg cgaggataat aaatttttta tcctctatat gacaaatcaa acgataatcg
     1381 ccgattcggc atctccatag accttttctg ttttcaatca gagttttttt gcatgttccg
     1441 agattttcgc agtctaaaag gtttttttca atccaggcat taatcatcaa ttttgtatag
     1501 cggtcgagtt ttttaaattc tctttcaaat cttgatgtca cttcaatcga atatgtcatg
     1561 tctgaagctc tttccaaaac tctttaatag ggcggctttg ttttccgctg tcctcatatt
     1621 ctttgtacgc agcatcataa atcgcaatat catattggtc ttcaattttc tcaaacaaag
     1681 ctcttttgaa agcctccgcc aatgtgtacg aatgtagctt tgcatagctt tccgccaatg
     1741 ctctttcttc ttcagttaat ctgattgaaa aggccatgtt atgtcacctc catctccata
     1801 gtacattgta ctgcaaaaat aaagaattta aagcattttt gcagtctgtt ttaaagtttc
     1861 agttactaat atgcttatta caatagaaaa actgtgtatt ctt
//
LOCUS       DAOPWC010000319         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_66391_length_1897_cov_3.722584, whole genome shotgun sequence.
ACCESSION   DAOPWC010000319 DAOPWC010000000
VERSION     DAOPWC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66391_length_1897_cov_3.722584"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..961
                     /gene="mltG"
                     /locus_tag="PKJ65_06650"
     CDS             59..961
                     /gene="mltG"
                     /locus_tag="PKJ65_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ41549.1"
                     /translation="MNDTYFWVYSLLMGNDGNYKSGNHIFDASVTYDSGSIDIVTAQG
                     YEMLIYILSSDSVPNPSKKIFFTEGLTMAQTVEKFTENDIRTKEEFYEVCNNYEFEYD
                     FVKQIPKDSRRTNRLEGYLFPDTYIFDVTVNAEEDVKKMLDNFNSKFNETYRAKAQAL
                     GYTIDQVVIIASLLEKEASTMDERAMIAGVLYNRLKSKETYLNYLQVDATIQYIYLNE
                     TGKLKEQLTNEDHEIMNSYNTYMYKGLPPGPICNPSLASIAAALYPETHNYFYYVAKN
                     DGTDAHAYATTLEQHNYYVKLYQK"
     gene            964..1617
                     /locus_tag="PKJ65_06655"
     CDS             964..1617
                     /locus_tag="PKJ65_06655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNZ41550.1"
                     /translation="MEKRFNIVNNDVEKYIQDTLAPLRDNLQVMYVNALKNGVPVVRL
                     EVLRVLETYLILKKPYSLLELGTATGFTSIFFSEYLKDNGYIDTVENDLEMVKRAREN
                     IKEMAPDKNINVIYAEACDYLKNIHKKYDIIFIDAAKGHYEEYFDICSNLVNENGIIF
                     SDNVLYRGMVAKGDKIPHRQKTLVERLHRYLDKITKDERFCTSILSVGDGLSVSVKK"
     gene            1650..>1897
                     /locus_tag="PKJ65_06660"
     CDS             1650..>1897
                     /locus_tag="PKJ65_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNZ41551.1"
                     /translation="MEKLRIAYAYGADAVYLGLNKFSLRDKADNFSYEELEHAVAYAR
                     ALGKRIYVTVNILPKNKDLEEIKKTFDRLSDILPDGII"
BASE COUNT          662 a          334 c          395 g          506 t
ORIGIN      
        1 cgtcattacc cattaacaac gaataaaccc agaaataagt atcgttgatg ataatatcat
       61 caacgatact tatttctggg tttattcgtt gttaatgggt aatgacggca actataaatc
      121 cggcaatcac atttttgatg cttcggtcac ttatgactcg ggaagtatag atattgttac
      181 ggctcaggga tatgaaatgc ttatttacat tctctcttcg gacagtgttc ccaatccctc
      241 caagaagatt ttctttaccg aaggtcttac aatggcacag acagtggaga aatttacgga
      301 aaacgacata cgcacaaagg aagagttcta tgaggtctgc aataattatg agttcgaata
      361 tgattttgta aagcagatac ctaaggattc caggcgtacc aaccgtctgg aaggctattt
      421 attccccgac acatatattt tcgatgttac cgtcaatgcc gaagaggatg ttaaaaaaat
      481 gctggataac ttcaacagca agtttaatga gacatacaga gcaaaagcac aagctttggg
      541 atatacaata gaccaggtgg tgattatcgc atcgctgctg gaaaaagaag catccacaat
      601 ggatgaaaga gcaatgatag cgggtgttct atacaacaga ctgaaatcca aagaaacata
      661 tctcaattat cttcaggtgg atgcaacgat acagtatatt tacctcaatg agacgggaaa
      721 gcttaaggag caattgacca acgaagatca tgagataatg aacagttaca acacctacat
      781 gtacaaagga ctgcctccgg gaccgatatg caatccttct ttagccagta tagcggccgc
      841 tttatatccc gaaactcata attacttcta ttatgtggcg aaaaatgacg gaaccgatgc
      901 tcatgcatat gccaccaccc ttgaacagca taactattat gttaagttat atcagaaata
      961 gccatggaaa aacgcttcaa catagtcaat aacgatgtgg aaaaatatat acaagacaca
     1021 ttagcaccgc ttagggacaa tctgcaagtg atgtatgtga acgctttaaa aaacggtgtt
     1081 ccggttgtgc gattggaagt cttaagagta ctggaaacct accttattct taaaaaaccg
     1141 tattcattac ttgaactggg caccgcaact ggttttacaa gtattttctt ttccgaatat
     1201 ctcaaagata acggttatat tgataccgtg gaaaatgatt tggaaatggt aaaaagagca
     1261 cgagagaata taaaggagat ggcacccgat aagaatataa atgtcatata tgcggaagca
     1321 tgcgactact taaaaaacat ccataaaaag tatgatatta tattcataga tgctgcaaaa
     1381 gggcattatg aagaatattt tgatatatgt tcaaaccttg tcaatgaaaa cggcataatc
     1441 ttttccgata atgtcttata caggggaatg gttgccaaag gcgataaaat acctcacaga
     1501 cagaaaacac tggtggaaag attgcataga tatctggata agattacaaa ggatgaaagg
     1561 ttttgcacat cgatattatc cgtaggagac ggattatcgg tttcggtaaa gaaatgagag
     1621 cgatagaact tctggcaccg gccggaaata tggaaaaatt aagaattgca tatgcatacg
     1681 gagcggatgc ggtatatctg ggcttaaata agttcagttt acgcgataaa gcggataatt
     1741 tcagttatga ggaactggaa catgctgttg cctatgcaag agccctcgga aagagaatat
     1801 atgtcacggt taatatactt ccgaaaaaca aggatttgga ggagattaaa aagacatttg
     1861 accgattgtc agatatttta cccgatggta tcataat
//
LOCUS       DAOPWC010000320         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_66801_length_1889_cov_3.483097, whole genome shotgun sequence.
ACCESSION   DAOPWC010000320 DAOPWC010000000
VERSION     DAOPWC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66801_length_1889_cov_3.483097"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      293..505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            612..1346
                     /locus_tag="PKJ65_06665"
     CDS             612..1346
                     /locus_tag="PKJ65_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNZ41552.1"
                     /translation="MQVKGIKKHKGKIIAAAVILCVLVVAYIWGGNYAVSDISYNFAS
                     PSPTPCQREGSSEGVFEGSKSYSEQLGMVFDKETGKDRYLTDPVPEGKPIPVEPQDAV
                     ITDTAHTCILSVRCDTILNNMKYLDKEKVELVPEDGVIFPATEVTFYEGESVFNILRR
                     EMKKAGIHMEFVNTPMYNSVYIEGINNLYEFDVGELSGWMYKVNGWFPNYGASRYQLK
                     AGDVVEWVYTCNLGRDVGGYYAMGEE"
     gene            1343..>1889
                     /locus_tag="PKJ65_06670"
     CDS             1343..>1889
                     /locus_tag="PKJ65_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ41553.1"
                     /translation="MMKDTFSTYHPAVNFLYFTLILVFSMFFMHPVCLAISLLSALCY
                     SIYLNGKKALFFNLKYMLPLLIITALINPAFNHEGGTILTYLRDGNPLTLESITYGIA
                     AAVMLITVISWFSCYNEVMTSDKFVYLFGRIIPALSLILSMALRFVPRFKAQIKVVSD
                     AQKCIGRDVSNGSILQRAKHGI"
BASE COUNT          533 a          356 c          425 g          575 t
ORIGIN      
        1 acgacctcca gcgtgacagg ctggcattct aaccaactga actaccaggc cgcataacgg
       61 gtaaaaattg ctcttaacca gatcattaca atatattctc ttaaaaaagt acaatcagac
      121 cttcattttt aggtcgctta ttgagtatac aaaataggcg tatttttgtc aacagaaaaa
      181 aaccggtgtg tcaaacaaac aaagatgttg ttatacttcc ttaattatga tttaattaaa
      241 gtcggtaatg tcttttagaa gacattgaaa agagtttatg taagctcttt aaaaattaaa
      301 tatctctttt tgtggtctca cgatgtgaga ctgaacaagg gaatcgggtg caaagcccga
      361 acggtaccgc cgctgtaagc gcccataatc aatgctcgtt gacgaaagtc ggtcactggg
      421 aaaccgggaa ggcagagcat ggttagcaga ctattagtct tgcgaagcgt tagtcagaag
      481 acctacaaga agatgctgtg ctctttttgg agtaagtgcg tttagcatga atacggttgc
      541 aacagttttt tgcggttgtg accgtttttt aacagggatg tggcgggtgc aaatatttgg
      601 gaggataaag gttgcaggta aagggcataa aaaagcataa gggaaaaata attgcagcag
      661 cagttatact gtgtgtcctc gttgttgcat atatttgggg cggcaattat gctgtatcgg
      721 atatttcata taactttgca agtccttctc ctacaccttg tcaaagagag ggttcttcgg
      781 agggtgtgtt tgaaggttcc aaatcctatt ccgaacagtt gggtatggta tttgataagg
      841 aaacaggcaa ggacagatat cttaccgacc ctgttcctga aggaaaaccc atacccgtag
      901 aaccccaaga tgccgttata accgacactg cacatacctg tattctttct gtccgttgcg
      961 ataccatatt gaataatatg aaatatttag acaaagaaaa ggtagagctt gtgccggaag
     1021 acggagttat tttcccggct accgaagtaa ctttctatga gggtgaatcg gtctttaata
     1081 ttcttcggag agaaatgaag aaagcaggaa ttcatatgga gtttgtgaat actcccatgt
     1141 acaacagtgt atatatcgaa ggtatcaata atctttacga gtttgatgta ggtgagcttt
     1201 ccggctggat gtataaggtg aacggttggt ttcccaatta cggcgcttcc cgttatcagt
     1261 taaaagccgg tgatgtggtt gaatgggtat atacctgtaa tttaggacgc gatgtgggcg
     1321 gatattacgc tatgggggaa gaatgatgaa agacacgttt tccacatacc accctgcagt
     1381 aaactttctg tatttcactt taatactggt gttttccatg ttttttatgc accctgtttg
     1441 tcttgcaata tctttactgt cggctttatg ttattcaatt tatttgaacg gtaaaaaagc
     1501 tttattcttc aatcttaaat atatgttgcc gctacttatc attaccgccc ttataaaccc
     1561 cgcttttaac catgaaggcg gcaccattct tacctatctt agagacggca atcctttaac
     1621 gctggaatcc ataacatacg gaatagccgc agcggttatg cttattaccg tcatatcctg
     1681 gttttcctgc tataatgagg ttatgacttc cgataagttt gtgtaccttt tcgggcgtat
     1741 tattcctgct ctgtcgctga tattatccat ggctttacgt tttgttccgc gctttaaggc
     1801 acagattaag gtggtttccg atgcgcaaaa gtgtataggg cgggacgtct ccaacggaag
     1861 tattttacag cgggcaaagc acggtatac
//
LOCUS       DAOPWC010000321         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_67460_length_1877_cov_3.620746, whole genome shotgun sequence.
ACCESSION   DAOPWC010000321 DAOPWC010000000
VERSION     DAOPWC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67460_length_1877_cov_3.620746"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..598)
                     /locus_tag="PKJ65_06675"
     CDS             complement(104..598)
                     /locus_tag="PKJ65_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ41554.1"
                     /translation="MEFIIRESVTDQDKHGKAYVHYTAWNETYTGLMPQGFLDGRSLE
                     KCIKNAYEYPKNTFVAVAEEKVVGFALYIPESRELVSIKPSSEVVAIYVLQKYQRNGI
                     GEALLKQCIKVLPRNPIAIFVLKGNEKAINFYKKQGFKFTGKTVEQNVKGGTLVELEM
                     VLER"
     gene            755..982
                     /locus_tag="PKJ65_06680"
     CDS             755..982
                     /locus_tag="PKJ65_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HNZ41555.1"
                     /translation="MAEISYKIEKEIGVLSEAPSGWTRQINLVSWNGREAKLEIRDWA
                     PNRERAGRGMTFTKDEIKKLIKFLVKAELDK"
     gene            1114..>1877
                     /locus_tag="PKJ65_06685"
     CDS             1114..>1877
                     /locus_tag="PKJ65_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNZ41556.1"
                     /translation="MKISVQKLYDSPNDYKDKTVTVEGWVRTIRSSKDFGFIELNDGT
                     CFKNIQIVFENNVVNNYAEVEKLPVASSISVTGKIILTPEAKQPFEIKAEDIEICGVS
                     ASDYPLQKKRHSFEYLRTVAHLRPRTNTFFAVFKVRSVLAQAIHTFFQDRGFVYVHTP
                     IITASDCEGAGEMFQVTTLDLDRIAGSKEKVDYSKDFFGKQTNLTVSGQLSVETYCMA
                     FRNVYTFGPTFRAENSNTPRHASEFWMIEPEMAFAD"
BASE COUNT          574 a          360 c          393 g          550 t
ORIGIN      
        1 accgttgcac gcacgattgc ttcattgaca gagaaaaaat atattgagag agtggggaat
       61 aataaggttg gattctggaa agttatacgg taatcggtac cgtctatctt tccagcacca
      121 tttccagctc taccaatgtg ccgcctttga cattctgctc cactgttttt cctgtgaact
      181 taaaaccctg ttttttataa aaattaatag ccttttcatt gcctttaagg acgaatatag
      241 ctatgggatt tcggggtaaa acttttatgc attgcttcag cagtgcttct cctataccgt
      301 ttctctgata tttctgcaga acatatatag ctacaacttc cgaagaaggt ttaatactga
      361 caagctctct ggattcgggt atatacaatg cgaacccgac aactttttcc tccgcaacgg
      421 caacaaaagt gtttttcgga tattcatatg cattttttat gcacttctca agactcctgc
      481 cgtctaaaaa tccctgcggc atcaatccgg tatatgtttc attccaagct gtgtaatgaa
      541 catatgcttt tccgtgttta tcctgatcgg ttaccgattc tcttattata aattccattg
      601 ctacacccat aaagccttaa tccgtcttat taccgcacaa atttgttttg tgaaacctat
      661 tatacaatgt ttttggggga attataacat tttattgata acatgccgct atatatgtat
      721 aataaatata aataacatat ataaggagaa taacatggcc gaaatcagtt ataaaataga
      781 aaaagagata ggtgttcttt ccgaagctcc ttcaggatgg acaagacaga taaacctggt
      841 aagctggaac ggcagagaag caaaactgga gattcgtgac tgggctccca acagagaaag
      901 agccggaaga ggcatgactt ttaccaagga tgagattaaa aagctcatta agtttcttgt
      961 aaaagcggaa ttggacaaat aaactgtatt tgttcataag ctgttcaggg tcggctgtcg
     1021 caggctaaaa tcggatataa tatatttatg tttccaacgc attatgcttt tggtataatg
     1081 ttttttgaat aatttacggg aaggacttat gaaatgaaga taagtgtgca aaagttatat
     1141 gacagtccga atgactacaa agacaaaaca gtgactgttg aaggatgggt aagaacaata
     1201 agaagttcca aagacttcgg ttttatagaa ctgaatgacg gaacctgctt taaaaatata
     1261 cagatagtat ttgaaaataa tgttgtaaat aattatgccg aggtggagaa actgccggta
     1321 gcctccagta tttccgtgac ggggaagata atactgactc ccgaagccaa gcagccgttt
     1381 gaaataaagg cggaagatat tgagatatgc ggcgtcagtg catcggatta tcctttgcag
     1441 aaaaagagac attcgtttga gtatctgcgt actgtcgcac acttacgccc cagaaccaat
     1501 accttctttg cggtattcaa agtgcgttcg gtacttgctc aggcaataca taccttcttt
     1561 caggacagag gatttgtgta tgtgcacacc ccgataataa ccgcatccga ctgtgaaggt
     1621 gccggtgaaa tgttccaggt aaccactctg gatttagacc gcattgccgg tagtaaggaa
     1681 aaagtggatt attccaagga ctttttcgga aaacagacca atcttacggt aagcggacag
     1741 ctttcggtgg aaacttactg tatggcattt aggaatgtat atacattcgg tcccacattc
     1801 cgtgcggaga attccaatac tcccagacat gcatcggaat tctggatgat tgagccggag
     1861 atggctttcg ccgactt
//
LOCUS       DAOPWC010000322         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_67629_length_1874_cov_3.431556, whole genome shotgun sequence.
ACCESSION   DAOPWC010000322 DAOPWC010000000
VERSION     DAOPWC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67629_length_1874_cov_3.431556"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKJ65_06690"
     CDS             <1..978
                     /locus_tag="PKJ65_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ41557.1"
                     /translation="KDKKLPAWRIAGAVLTALAFLATAYFTVSNMGDLSAIIKQGDLS
                     VVSFGKEMNIWHYVLLAVALAAELLLFFILLRPKHTKIDNEQVAADKRKLSKRTLLAA
                     AMILIAIPLTIYLGIYYLEDRKYYFISMLIILETMLPFVLIFEGRKPQARELIIIAVL
                     CAIGVAGRTAFFMLPQFKPVAAIVIIAGVAFGGETGFLVGAVTAFVSNMFFGQGPWTP
                     WQMFAFGIIGFLAGILFRKGILRRNTIALCIFGGLATFLIYGGIMNPASVLMYEAKPT
                     MEMFYVAYIRGIPFDLIHAVATVVFLFSIAKPMLEKLDRVKLKYGLIER"
     regulatory      1093..1283
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1506..>1874
                     /locus_tag="PKJ65_06695"
     CDS             1506..>1874
                     /locus_tag="PKJ65_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41558.1"
                     /translation="MKKRILSLLLALVVSIGMLPMSAMADPAVPDAKIDGVMYIPEMY
                     AYNIVDTVLEEDGDLVLPSSLTLDGIEEGTALSVYALAMDGNGDFYIKEAPIVDGKFT
                     MDSAFKTVITDPNYYERFYIL"
BASE COUNT          525 a          328 c          458 g          563 t
ORIGIN      
        1 aaagataaga agttacccgc ctggcgcata gcaggtgcag tgcttactgc cttagccttt
       61 cttgcaacgg catattttac cgtaagcaac atgggggatt tgtcagctat tattaaacag
      121 ggtgatttat cggtagtttc tttcggtaaa gaaatgaata tatggcacta tgttctgtta
      181 gccgttgcat tggcagcgga attacttctg ttctttattc ttcttcggcc taaacataca
      241 aagatagata acgaacaggt agctgcggat aaaagaaagc tttccaaacg tacacttctt
      301 gctgctgcaa tgatacttat agctatccca ttaactatct acttaggcat ctattattta
      361 gaagacagaa aatattattt tatcagtatg ctgataatat tggaaaccat gctgcctttt
      421 gtccttatat ttgaagggcg caaaccgcag gcaagagagt tgattataat tgcggtgctt
      481 tgtgccatag gagtggcggg gcgtacggct tttttcatgt taccgcaatt caagcctgta
      541 gcggctattg taattatagc gggagttgca ttcggaggtg agacgggatt tttagtagga
      601 gcggttaccg cctttgtgtc caatatgttt ttcggtcagg gaccgtggac accttggcag
      661 atgtttgctt tcggtataat agggtttttg gccgggatat tattcagaaa aggaatatta
      721 cggcgcaata ctattgcact ttgtatcttc ggcggattgg ctacattctt aatatacgga
      781 ggtataatga atcccgcatc ggtattgatg tatgaagcaa aacccacaat ggaaatgttc
      841 tatgttgcat atatccgggg tattcctttt gaccttatcc atgcggttgc caccgttgtc
      901 ttcctgtttt ccattgcaaa acccatgctg gagaaattgg acagggtaaa gctgaagtac
      961 ggacttatag aaaggtgaaa aagaaaaaaa agggtagcaa ttgcatgccc tttgtgatat
     1021 ggtttagtgg agcttgaggt ctcggtagtt tacgggattt caggagataa tttcgagtgc
     1081 tgtatgcggt ggtctgttct ttggaataag aacgcataca tgttaaaagg gaagccggtc
     1141 ggaatccggc acggacgctg ccactgtgaa ggtccgcact cccgcggtga aaaccggtca
     1201 ttgaatcgtt atgatttgat aaggcaaggg agagcataaa aaggaccgaa gtcaggagac
     1261 ctgctcggaa ttcatgccgt cacgcgttaa atgacagggg cattttgttt tgatgtgcga
     1321 agatacagcc gcacgatggt agatttttac tgtcgtgcgg tttttgcttt ttaacacaaa
     1381 ttgcatacca aaaaccaaaa acagcgaata taagttttta ataaagcttc aaggtggcag
     1441 atgattgaag cttaaaatgt aaaaaacatt tatgaatctt tgtgcacaga gaaaggatga
     1501 ggaaaatgaa gaagcgaatt ttaagtttat tgttagcact tgttgtaagc atcggaatgt
     1561 tacctatgag tgctatggca gatccggcgg taccggatgc aaagattgac ggtgttatgt
     1621 atatacctga gatgtatgcc tataacatcg tggatacggt tttagaggaa gacggtgacc
     1681 tggtgttacc ttcttctctt acacttgacg gcatagaaga aggcaccgcc ttatcggttt
     1741 atgcgttagc catggatggg aatggagact tttatataaa agaagctcca attgttgacg
     1801 gaaagtttac aatggattcc gcttttaaga cggttataac cgatcctaat tactatgaac
     1861 gtttttatat tctc
//
LOCUS       DAOPWC010000323         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_68622_length_1857_cov_3.272475, whole genome shotgun sequence.
ACCESSION   DAOPWC010000323 DAOPWC010000000
VERSION     DAOPWC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68622_length_1857_cov_3.272475"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="PKJ65_06700"
     CDS             <1..1629
                     /locus_tag="PKJ65_06700"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNZ41559.1"
                     /translation="ITLGISIYRPGPMKFIDMCLCNKNSPGGTVYEHELMKPILSDTY
                     GCMIYQEQIMQICRDMAGYTTGESDLVRRLMSKKKMSALLKEKDRFVRGAEKNGIPTE
                     VANKLFDEMTDFGNYAFNKSHGVAYAYISYQTAYLKCYYPVEFMAATMNSFIATREKL
                     AEYIEECRSINISVLPPDINKSDVKFTVEGGNIRYPLAAINGVGIKACETIVNNRNQQ
                     GEYRSFVDFIKRNSKSDVNKKCIEGFIKAGAFDGLGNNRCELFSCYEEVVDSINEQQK
                     KNLDGQISLFAMVEDPTEHEYEIRKREEFDLSKKLIMEKEAIGLYMTGNPLDEYRSVL
                     KEMNLFKCSYKNIDETERDSVRLVDNERVKVAGIINNVQRKLTKNNEMMAFAVIEDLY
                     DTMEIIIFPRTLEKYSSLLVPENKVIINGKISLKQDTVKIIAEDIVELNIKKDDSITV
                     NEDTADYRNNKENMRTDNGISFSDNQKLYLNGDNINLSQLEAFVKYFTGNSALTIIKN
                     VNGSKMVKTMYYNQTVEIVNEMAELFGKENMAFK"
     gene            1670..>1857
                     /locus_tag="PKJ65_06705"
     CDS             1670..>1857
                     /locus_tag="PKJ65_06705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ41560.1"
                     /translation="MKTIGVLTSGGDAPGMNAAIRAVVRTGIEDGITTYGISRGYEGL
                     INGELEKLNARSVSGILQ"
BASE COUNT          698 a          286 c          428 g          445 t
ORIGIN      
        1 ataacattgg gtatttccat atacagaccg ggaccgatga agtttataga tatgtgtctg
       61 tgtaacaaga acagtcccgg aggtaccgtt tatgaacatg aactgatgaa acccattttg
      121 agcgatacat acggatgcat gatatatcag gagcagataa tgcagatatg tcgcgatatg
      181 gcgggatata cgacgggtga atcggacctg gtcagaagac tgatgtcaaa aaagaagatg
      241 tcggcattgc tgaaagaaaa agacagattt gtcagaggag cggaaaaaaa cggtataccc
      301 acggaggtag ccaataagtt atttgatgaa atgaccgatt tcggaaatta cgcatttaac
      361 aagtcccacg gagtggcata tgcatatatt tcctatcaga cggcatatct gaaatgctat
      421 tatcccgtag aattcatggc ggcaaccatg aacagcttta ttgcaacaag agaaaaactt
      481 gccgaataca ttgaggaatg ccggtccata aatatatcgg tacttcctcc tgatattaat
      541 aaaagtgatg tgaagtttac cgttgaggga ggaaatatca gatatccttt agccgctata
      601 aacggtgtag ggataaaagc atgcgaaacc atagtcaata acagaaacca acagggagaa
      661 tacaggagtt tcgtggattt tattaaaaga aacagtaaaa gcgatgtcaa taaaaagtgt
      721 attgaaggct ttataaaagc aggcgcattt gacggattgg gaaacaacag atgtgaatta
      781 ttcagctgct atgaagaggt tgtggattcc atcaatgaac aacagaaaaa gaaccttgac
      841 ggtcagatat cgctttttgc aatggtggaa gatccgacgg aacatgaata tgagataaga
      901 aagagagagg aattcgacct gtcaaagaaa ctcattatgg aaaaagaagc gataggctta
      961 tacatgacgg gcaatccttt agacgaatac agaagcgttc tgaaagagat gaatcttttc
     1021 aaatgcagtt ataaaaatat tgacgaaacc gagagggatt ccgtaagact tgtagataac
     1081 gaaagagtaa aagtggccgg gataataaac aacgtgcaaa gaaaactcac aaagaataat
     1141 gaaatgatgg cctttgcggt tatagaagac ttatacgata ccatggagat aataatattc
     1201 cccagaacct tggaaaaata ttcttctttg cttgtaccgg aaaataaagt gattataaac
     1261 ggaaaaataa gcctgaaaca ggataccgtt aaaataatag cggaagatat tgttgagttg
     1321 aatattaaga aagatgacag tattaccgta aatgaagata ctgccgatta ccggaacaat
     1381 aaggaaaaca tgcgaacgga taacggtatc agtttctccg ataatcaaaa actctattta
     1441 aacggagaca atataaatct ttcgcagctg gaagcttttg taaagtactt taccggaaac
     1501 tccgctttga ctatcattaa gaatgtaaac ggcagtaaaa tggttaaaac catgtactat
     1561 aatcagactg tcgaaattgt taatgaaatg gcggaacttt tcggaaaaga aaatatggca
     1621 tttaagtaga tatgtgataa aattttaatt caggagacgg attatgaaaa taaagacaat
     1681 cggagtttta acaagcggag gagatgcacc aggaatgaac gcggcaataa gagctgtcgt
     1741 aagaacgggc atagaagacg gaattaccac atacggtata agcagaggat acgagggttt
     1801 gattaacgga gagttagaaa aactcaacgc aagaagcgta tcgggcatat tgcagag
//
LOCUS       DAOPWC010000324         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_68685_length_1856_cov_3.756802, whole genome shotgun sequence.
ACCESSION   DAOPWC010000324 DAOPWC010000000
VERSION     DAOPWC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68685_length_1856_cov_3.756802"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1135
                     /gene="dprA"
                     /locus_tag="PKJ65_06710"
     CDS             221..1135
                     /gene="dprA"
                     /locus_tag="PKJ65_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ41561.1"
                     /translation="MTNISVTIQIMNEAGYIFSCIDFLSPNEKYRLYMASKSVYEIFD
                     MNNDDLLYLSKISDVPYDKLKTVRNISYPKYKKGNSDIKITDIYSDDYPKMLKETYGA
                     PIVLYHIGDIPKECMVSVVGSRKPGPYGIRCAEVLSVNLSLQGFTVVSGMAKGIDGIC
                     HMAALRHGNSVAVLGNGLNICYPSENRYIYDMLIKKGCLISEYPPDSKPGKINFPMRN
                     RIISGLSFCTIVVEAGIKSGSLITANCALSQNREVYCFPNYIDTGFNGTNQLIKEGAG
                     IITDIDDFCRELNAIEIHWKTIYYCKSN"
     gene            1149..>1856
                     /locus_tag="PKJ65_06715"
     CDS             1149..>1856
                     /locus_tag="PKJ65_06715"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNZ41562.1"
                     /translation="MSKNLVIVESPAKANTIKKFLGNGFTVMASFGHVRDLPEKAFGI
                     DVENHYQPSYEILSGKKEVVDRLKKAVKESDRTYLATDPDREGEAISYHLAHLLKIDP
                     QSKCRIVFNEITKPAVTNALNETRPIDMDMFYAQQARRILDRIVGYKTSPLLWKKIKK
                     GLSAGRVQSVALELICDREEEIRAFVPEEYWNLMLKLSKISDDQIFMAKFYGKDNEKV
                     KLSNEGEANEVYKYVSNN"
BASE COUNT          649 a          310 c          391 g          506 t
ORIGIN      
        1 caaaagaacc gggaagtcat aaagcacatg aactgctcca tacgcattac cagaagagag
       61 agaagcatct taacaaataa tagtttctta ttcgggtaat gttaaatatt tgaagaataa
      121 gcaaaagcgg tagaaatacc gctttttctg tagaacaata aaaatcagta aattactcaa
      181 gaattaaaag tatcagcata atcattattg tctgttcatt atgacaaaca taagtgttac
      241 cattcagata atgaatgaag caggatatat cttctcatgt atagattttt tatctcccaa
      301 cgaaaaatat cgcctgtaca tggcgagtaa atctgtgtat gaaatatttg acatgaataa
      361 tgatgatttg ctttatctct cgaagatttc cgatgttcct tatgataaac tcaaaaccgt
      421 ccgaaatata agttatccta aatataaaaa agggaatagt gatataaaga ttacggatat
      481 ctattcggat gattatccca agatgcttaa agagacatac ggtgctccca tagtcctata
      541 tcacattgga gatataccga aagaatgtat ggtttcggtt gtgggctcca gaaaacccgg
      601 tccttacggt ataagatgtg cagaagtatt atcggttaac ttgtctttac aaggctttac
      661 ggtggtcagc ggcatggcta aaggaataga cggtatctgc catatggccg cattacggca
      721 cggtaattcc gttgcggtct tgggtaacgg tttaaatata tgttatccct cggaaaaccg
      781 atatatatat gatatgctga ttaaaaaagg atgcctaatt agcgagtatc ctcccgacag
      841 taaacccggg aagattaatt ttcccatgcg aaacagaata atatccggct tatcattttg
      901 tacaattgtg gtggaagccg gcataaaaag cggttcattg attacggcta attgcgcatt
      961 gtcacagaac agggaagtgt attgttttcc caactatatc gataccggtt ttaacggaac
     1021 aaatcaatta atcaaagaag gtgcgggtat tattacggat atagatgatt tctgccggga
     1081 attaaatgct attgaaattc attggaaaac catttattat tgtaaaagca attgatttgg
     1141 aggaaattat gtcaaaaaac ctggttatag tggaatcgcc tgccaaggcc aatacaatta
     1201 aaaagttttt aggtaacggt tttaccgtca tggccagttt cggtcatgtg cgggatttac
     1261 cggaaaaagc atttggaatt gatgttgaaa accattacca gcccagctat gaaattttaa
     1321 gcggaaagaa agaagttgta gacagactaa agaaagccgt aaaggaaagc gacagaacat
     1381 atcttgcaac cgaccccgac cgcgaaggtg aagctatttc ctatcatctg gctcatttac
     1441 tcaaaataga tcctcagtca aaatgcagaa ttgttttcaa cgagataaca aaacccgcag
     1501 taaccaatgc tttgaatgaa acaagaccta tagatatgga tatgttctat gcacaacagg
     1561 caagaagaat actggacaga atagtaggtt acaaaaccag cccgctgtta tggaagaaga
     1621 ttaagaaggg cttaagtgcc ggcagggtac agtcggtggc attggaactg atatgcgaca
     1681 gggaagagga aataagggca ttcgttccgg aagaatattg gaatctcatg ctgaaactca
     1741 gtaaaatttc cgatgatcag attttcatgg caaaattcta cggaaaggat aatgagaaag
     1801 ttaagctgtc aaatgaaggc gaagctaatg aagtatataa atatgtaagt aataac
//
LOCUS       DAOPWC010000325         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_68809_length_1854_cov_3.030573, whole genome shotgun sequence.
ACCESSION   DAOPWC010000325 DAOPWC010000000
VERSION     DAOPWC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68809_length_1854_cov_3.030573"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKJ65_06720"
     CDS             <1..530
                     /locus_tag="PKJ65_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41563.1"
                     /translation="IQHVYKCDDAGAEYYAIFDKTLTDYSGWAFPKDGTFTIGMAFPD
                     GADGNRLFDQLIEKMKNDGLSIGQAVSKEGSLLLRPDGYPKTFSKNNNTLFLAGEAAG
                     YVSPSSAEGFSFAFNSACALAKSFEKGGNISVNYRNKTGKMRTKLFFKVIKAHLLYNQ
                     FVRKMIMACNIKAIR"
     gene            541..1335
                     /locus_tag="PKJ65_06725"
     CDS             541..1335
                     /locus_tag="PKJ65_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNZ41564.1"
                     /translation="MALKDIKCFVLDMDGTIYLGNQLFDYTKEFLATAQQCGIDIWYY
                     TNNSSKNAKFYINKLKKMGIVCEERKMLISNQVIIKHLLEHHQESRVYVAGTQFLIDD
                     FKEAGINVADTDCDVVVMGFDMTLTYEKLVKACDFVRYGATLYGVNMDYNCPTETGFI
                     PDCGSICQLVYASTGVSAEFFGKPSKHTADFIFEKTGYDKKSLAIIGDRVYTDIALGA
                     YNGFTSILVLSGESSLEDVKNSDTKPDLIYASLKEISADLKKIYKL"
     gene            complement(1340..>1854)
                     /locus_tag="PKJ65_06730"
     CDS             complement(1340..>1854)
                     /locus_tag="PKJ65_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41565.1"
                     /translation="ICVAIIFFFVLIVVLDKKSIPVLRPAREPDLLKKHMLDIKANFP
                     AYVNNILSLDEGKDLKKSNEPLDIGVIILKNYGNSSAVDIQVTNIGSYKVEEDFSNKY
                     VSLQPGESVAVLVYIPKDLLSSVKVSLVKVKSKNYANSTRTEKFSIISDSELSYRISE
                     KCTLHPFIQM"
BASE COUNT          587 a          327 c          394 g          546 t
ORIGIN      
        1 ctattcagca tgtgtataag tgtgacgatg ccggtgctga atattacgca atatttgaca
       61 aaacattgac cgattattca ggatgggcat tccccaaaga cggtactttt actataggta
      121 tggcatttcc cgacggggcg gacggtaaca gactgtttga tcaacttatt gaaaaaatga
      181 agaatgacgg gttatcaata ggtcaggctg tgagtaaaga aggctcactg ttgttacgac
      241 cggacggcta tccgaaaaca ttttcaaaaa acaacaatac attgtttctt gcaggtgaag
      301 cggcaggcta tgtgtcgccc agttccgcag aaggattcag ttttgctttt aacagtgcgt
      361 gtgcgcttgc aaagagtttt gaaaaaggcg gtaatatatc ggtaaactac aggaataaga
      421 ccggaaaaat gcggacaaag ctgtttttta aggtaattaa agcacattta ttatataatc
      481 agtttgtccg gaagatgata atggcttgta atataaaggc gataagatag gggagatact
      541 atggcattaa aagacataaa gtgttttgta ttggatatgg acggaactat ttatctggga
      601 aatcaacttt tcgattatac aaaagagttt ttagccacgg cacagcaatg cggaatagat
      661 atatggtatt atacaaacaa ttcctcgaag aatgctaagt tctatatcaa taaactgaag
      721 aaaatgggga ttgtttgtga agagagaaag atgcttatct ccaatcaggt aatcataaag
      781 catcttctgg agcatcacca agaaagcaga gtatatgttg ccggtacaca gtttctgata
      841 gatgatttta aggaagccgg aattaatgta gcggatacgg attgcgatgt ggttgttatg
      901 ggatttgata tgacgctgac atatgaaaag cttgtcaaag cctgtgattt cgtgcgttac
      961 ggtgcaactt tatacggtgt caatatggac tacaactgtc ccaccgaaac cgggttcatc
     1021 cctgactgcg gctccatatg tcagttggta tatgcatcta cgggtgtaag tgcggaattt
     1081 ttcggaaaac cctcaaaaca tacggcggat ttcatatttg aaaagaccgg atacgataaa
     1141 aaatctctgg caataatagg agacagagta tacaccgata tagcattggg agcctacaac
     1201 ggttttacat cgatacttgt tctttccggg gagtcttctt tggaggatgt aaaaaacagc
     1261 gatacaaaac ccgacttgat atatgcttcg ctgaaagaaa tatcagcaga tttaaagaag
     1321 atatataaac tttaatgcac tacatctgaa taaaaggatg taaagtacat ttttcactta
     1381 tccggtaact caattcgctg tcggatatta tactgaattt ttccgttctg gtactgttgg
     1441 cataattttt cgatttcact ttgacaagcg atactttgac cgaacttaat aagtctttgg
     1501 gaatatagac cagaacggca acgctttcac cgggctgtaa agagacatat ttattggaga
     1561 aatcttcctc cactttataa ctccctatat tagtaacctg aatatccacg gcggaactgt
     1621 ttccgtagtt tttcagtatt atcaccccta tatccagcgg ttcattgctt tttttcaaat
     1681 ccttgccttc atcaagagaa agtatattgt ttacatatgc cgggaagttg gctttgatat
     1741 ccaacatatg ttttttcaat agatccggtt ctcttgccgg tctcagtacg ggaatacttt
     1801 ttttatccag aaccactatc agaacaaaaa agaaaataat agccacacaa ataa
//
LOCUS       DAOPWC010000326         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_69632_length_1840_cov_3.252101, whole genome shotgun sequence.
ACCESSION   DAOPWC010000326 DAOPWC010000000
VERSION     DAOPWC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69632_length_1840_cov_3.252101"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="frr"
                     /locus_tag="PKJ65_06735"
     CDS             <1..488
                     /gene="frr"
                     /locus_tag="PKJ65_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ41566.1"
                     /translation="SVIRAGRANPSVLKNIYVDYYGQKTPLLQIGNISSPEPRLLVIQ
                     PWDISIMKDIEKAIMASDLGINPQSDGRVIRLLFPKLSQERRNELVKKVKAFGEDAKV
                     RIRNIRREDMDKYKKQKKDSEITEDDYTVIEKDIQKLTDESTAKIDDIVKEKTEEIME
                     V"
     gene            522..1232
                     /locus_tag="PKJ65_06740"
     CDS             522..1232
                     /locus_tag="PKJ65_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNZ41567.1"
                     /translation="MQLPQHIAIIMDGNGRWAKQRRMPVSYGHKCGADAIQNLIEQSD
                     ELGIRYITAYTFSTENWKRPKSEVAYLMTLLQDFLNDTSGRLERSNIRIRFIGSRDRI
                     KRKMVESMERIERISAEKTGLTFIIALDYGARQEITQAVKRISSDVKAGILDISQINE
                     QLVSDYLYTKDIPDPDLIIRPSGEKRLSNFLLYQASYSELWYSDILWPDFTIEHLKQA
                     ITDYNNRKRRFGGRTDED"
     gene            1222..>1840
                     /locus_tag="PKJ65_06745"
     CDS             1222..>1840
                     /locus_tag="PKJ65_06745"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ41568.1"
                     /translation="MKTRVISALLALLILVFIYVFPGLPTYIVLFLISALMFYEFLHV
                     TNEKLTLGYYIICALFTFLYYTNLYLTIGLAIIPQEYLYTLNILLTVFFALTILVASM
                     FYKIKTFREVTYTIFGYIYTVVLLSFVVLILKMQKGAFLFVYVWLGALGCDIWAFLIG
                     RSFGKLKILSEVSPSKTLEGSIGGAVGSILMITAYTIVINRYFNTQ"
BASE COUNT          627 a          289 c          390 g          534 t
ORIGIN      
        1 tttcagttat acgagccgga agagcaaatc cgtcggttct gaaaaatatt tatgtggatt
       61 attacggaca gaagacaccg ttacttcaga taggcaatat ttcttctccg gaaccgaggt
      121 tattggttat acaaccttgg gatataagta ttatgaaaga catagagaaa gcaataatgg
      181 cttcggattt gggtatcaat ccgcaaagtg acggaagggt aattcgtctg ttattcccta
      241 aattatctca ggaaagaaga aatgaattag ttaaaaaggt caaagcgttt ggagaagatg
      301 caaaggtcag gataagaaac atacgcagag aggatatgga taagtataag aagcagaaaa
      361 aagattctga gattactgag gacgactata ccgtaattga aaaagatata cagaagctta
      421 cagacgaatc tacagctaag attgatgata tagtaaaaga aaagaccgaa gagataatgg
      481 aagtctgata tctcatacgg aaacggagaa ttaaacgata tatgcaacta ccacaacata
      541 tcgccattat tatggacgga aatggaagat gggcaaaaca aagaaggatg ccggtcagtt
      601 acggacataa gtgcggtgcc gatgcaatac agaatctgat agaacagagt gatgagttgg
      661 gaataagata tattacagct tatacttttt ctacggaaaa ctggaaacgt cccaaaagcg
      721 aagttgcata tctgatgact ttacttcagg attttttaaa tgacacatcc ggtcgtctgg
      781 agcgttctaa tatacgtatc agattcatag gatcgagaga cagaataaaa agaaaaatgg
      841 tagaaagtat ggagaggatt gaaagaatat ccgccgaaaa aaccggcctt accttcataa
      901 tcgctttgga ttacggtgcc agacaggaga taacacaggc ggtaaaaaga attagcagtg
      961 acgtgaaagc cggtattctg gatatttcgc agattaatga gcaacttgta agtgactatc
     1021 tgtacacaaa agatattccc gatcccgacc ttataatccg cccgagcggt gagaagcgat
     1081 tatccaattt tcttctgtat caggcatcat actcggagtt gtggtattcg gatattttat
     1141 ggccggattt tactatagag catttaaagc aggccataac ggattacaac aacagaaaga
     1201 gacgctttgg aggaagaacc gatgaagact agagtaataa gtgctttgct ggcattattg
     1261 attttagtat ttatatatgt ttttcccggt ttgcctacat atatagtttt gtttttaata
     1321 tcggcattga tgttttatga gtttcttcat gttacaaatg aaaaacttac cttaggatac
     1381 tacataatat gtgcattatt cactttcctt tattatacaa atctctatct gactatagga
     1441 ttggcaataa ttcctcagga atatctgtat actcttaata ttttgcttac cgtctttttt
     1501 gcactgacaa tactggtagc atccatgttc tataagatta aaacattcag ggaagtaaca
     1561 tataccatct tcggttatat atacacagta gttttattat cttttgttgt acttatactt
     1621 aagatgcaaa aaggagcatt tctgtttgtc tatgtatggt taggtgcttt gggatgtgat
     1681 atctgggctt ttcttattgg cagaagtttc ggaaaattaa aaatattaag tgaagtaagt
     1741 cccagtaaga cactggaagg ctcaataggc ggagcggtag gttccatctt aatgattacc
     1801 gcatatacaa tagtaattaa cagatacttc aatacacaga
//
LOCUS       DAOPWC010000327         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_69756_length_1838_cov_3.150869, whole genome shotgun sequence.
ACCESSION   DAOPWC010000327 DAOPWC010000000
VERSION     DAOPWC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69756_length_1838_cov_3.150869"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKJ65_06750"
     CDS             complement(<1..227)
                     /locus_tag="PKJ65_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNZ41569.1"
                     /translation="MKRLTKKENFIMQMFWENGPMTVNELLELYPEPKPHFNTVSTQV
                     RNLEANGFFKHRREGMTYRYFPIITPQEYSNA"
     gene            485..1480
                     /locus_tag="PKJ65_06755"
     CDS             485..1480
                     /locus_tag="PKJ65_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ41570.1"
                     /translation="MDYSIYKAAEDRYESGMAYIRCGQSGILLPAVSLGLWHNFGSVD
                     DFDNALAIARYAFDNGITCFDLANNYGPLYGTAEENFGRIMDNSFRPYRDELFITTKA
                     GYDMWPGPYGNWGSRKYLISSLDQSLKRMRLEYVDLFYSHRYDPLTPLDETLQALVDT
                     VRSGKALYVGISNYPVDTLEYVFSYLKSHDTPCLIYQGRYNMLDRNVETSGILKVCVD
                     SGVGFTAFSPLAQGLLTDRYLNGIPSGSRMTRNRFLKRDLLTPELMTRLKGLEALALQ
                     RGQSLSAMALSWLLRDEKITSVLVGVSSIEQLKSNIRAQDNIAFSEDEINAIEEL"
BASE COUNT          523 a          352 c          387 g          576 t
ORIGIN      
        1 gcattactat actcctgagg ggtgattatt gggaagtaac gataggtcat gccctccctc
       61 cggtgtttaa agaatccgtt tgcttcaagg tttcgtacct gtgtggaaac ggtgttgaaa
      121 tgaggcttgg gttccgggta aagttcaagc aactcattga cagtcatggg accattctcc
      181 cagaacattt gcataatgaa gttctctttc tttgtaagtc ttttcatttc aaatagtttt
      241 attcaagaaa cttataccgg aactaatatc cggagaaagg tttcctttta ttaaacattc
      301 aattccgtac aaatatataa atattgttaa taatggttgt gcaaattgta cacttttttc
      361 agaaaaaaag aatttatttc tgtaaagata cattatttaa ataataaacc aaatagttga
      421 ccatattttg gacataaatt atttgtagtt ttgtagtgat aatttaagcc aataaatttt
      481 tattatggat tattcaatct ataaggctgc tgaagatcgt tatgaaagcg gtatggcata
      541 tatccgatgt ggccaaagcg gaatcttgtt gcctgccgtt tcactgggac tctggcataa
      601 cttcggcagt gtggatgatt tcgataatgc tcttgctatt gcccgatatg cttttgataa
      661 cggcataacc tgttttgacc ttgcaaataa ttatggaccg ctctatggta ctgccgaaga
      721 gaattttggg cgtatcatgg acaactcgtt tcgcccttac cgtgacgaac ttttcataac
      781 caccaaggcg ggatatgaca tgtggcccgg tccttacggc aactggggtt caaggaaata
      841 tctcattagc agccttgatc agagccttaa aaggatgcgg cttgaatatg ttgacctctt
      901 ctattcgcac cgatatgacc ctctcactcc gctggatgag actctgcagg ctttggtcga
      961 tacggtacgc agcggcaagg ccctttatgt gggaatatcc aactatcctg ttgataccct
     1021 ggaatatgtc ttcagttacc tgaagagcca cgatacacct tgtttaatat atcagggtcg
     1081 gtacaatatg ttggatcgca atgttgagac atcgggcata ctgaaggttt gcgttgactc
     1141 cggagtaggg tttaccgcct tttcgccgct ggcccaggga ctcctgacag accgttatct
     1201 gaatggcata ccatccggtt ccagaatgac ccgcaaccgg ttccttaaga gggatctact
     1261 cacgcccgaa ttaatgacgc gtcttaaagg gcttgaggcg ttagccttgc aacgcgggca
     1321 gtctttatcg gcaatggcac tgtcatggct tctaagggat gaaaagatca cttcggttct
     1381 ggttggagta agttcaatcg aacagttgaa aagcaacata agggcacagg ataacatcgc
     1441 cttttcggag gacgaaatta atgccataga agagctgtag aaatagtttc atttgaaaat
     1501 gatccgaatt gtgtaccatt cccgatttgc acaaccggcc ggaaagagtc aggcagatag
     1561 cagaggtttt taaccctttt gtataagccg ctattttaca gagtttccca aaacgggaaa
     1621 cttttctcat tttataaaaa tataaataat taataatcaa tgtgttaatc atttaaaacg
     1681 gaaaactttc aataagttga taaatatttt ttgtcaatta ttgttttata ttggatacat
     1741 tgtttaaatt tgtcattgct ttcggtcaac aagaccgaac gttatttttt tccgtttttt
     1801 aattattaaa aaaagtacta ataatatatc ccggaggc
//
LOCUS       DAOPWC010000328         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_69869_length_1836_cov_3.218978, whole genome shotgun sequence.
ACCESSION   DAOPWC010000328 DAOPWC010000000
VERSION     DAOPWC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69869_length_1836_cov_3.218978"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="ilvB"
                     /locus_tag="PKJ65_06760"
     CDS             complement(<1..1320)
                     /gene="ilvB"
                     /locus_tag="PKJ65_06760"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNZ41571.1"
                     /translation="MKATGTKVVIETLIEQGCDTVFGYPGGAVLSIYDELYRHSDKIR
                     HITCAHEQGAAHAADAYARVTGKTGVVIATSGPGATNLVTGIANAYLDSSPIVAITGN
                     VPLSLLGKDSFQEVDITGVTMPITKHNYIVKDVNELADVIRETFFIAQSGRPGPVLID
                     VPKDVQTAVCNFEHKKTVKKERECFVTEEQLLLAAKLISQSEKPYIYCGGGVVISQAG
                     EEIAKLAEKTDSPIGFSMMGLSAVSADNPYWLGMTGMHGRFASTKAHSEADLIIAVGV
                     RFSDRATGNRAKYTQNAKVIHIDIDPAEINKNISVHCSLVGDVKEVVTKLLDRLEKKT
                     NTQWMDAVQRMKNLETMRSDGDSAMHPEDIIEKVMEYTKDSTIVATDVGQHQMWVAQY
                     YRFKKPRTHLTSGGLGAMGFGMGAAIGGCVASGGKRTVLFTGDGSFGM"
BASE COUNT          477 a          475 c          330 g          554 t
ORIGIN      
        1 cataccgaaa gaaccgtctc cggtaaacag caccgttctt ttgcctcccg atgccacaca
       61 tcctcctata gccgctccca ttccgaaacc cattgcaccc aaacctccgg atgtaagatg
      121 agtacggggt tttttaaacc tgtaatactg tgcaacccac atctgatgct gaccgacatc
      181 ggttgcaaca atcgtactgt ccttagtata ttccattact ttttcaataa tatcctcagg
      241 atgcatagcg gaatctccat cggaccgcat tgtctccagg tttttcatcc tttgtaccgc
      301 atccatccat tgcgtatttg tctttttctc cagtctgtcc agaagtttcg taactacctc
      361 tttaacatcg cctaccaggc tgcagtgtac cgagatattt ttatttatct ccgcagggtc
      421 gatatcaata tgaataacct ttgcattttg tgtgtatttg gctctgtttc ctgttgcacg
      481 gtcggaaaaa cgtactccga cggctattat gaggtcagcc tccgaatgtg cctttgtgga
      541 tgcgaatctg ccgtgcattc ccgtcattcc cagccaataa gggttatcgg ccgatactgc
      601 tgaaagaccc atcatggaaa accctatggg tgaatctgtt ttctcggcca attttgctat
      661 ttcctcaccc gcctgtgata tgactacccc tccgccgcag tatatatacg gtttctcgga
      721 ttgagatatc agtttagctg ccaaaagaag ttgctcttcc gttacaaaac actctctctc
      781 tttctttaca gtttttttat gttcaaaatt acagactgca gtctgaacat ccttgggaac
      841 atctatcaac accggtcccg gtcttcccga ttgagcaata aaaaatgtct cccttataac
      901 atcggcaagc tcgtttacat ccttaactat atagttgtgc ttggttatgg gcattgttac
      961 tcccgttatg tccacttcct gaaaactgtc ttttcccagt aaagaaagag gtacgttacc
     1021 ggtaatggca acaatcggag acgagtcaag ataagcattt gctattccgg ttacaagatt
     1081 cgtggcaccc ggtcctgaag tggctattac cactcccgtt ttacccgtca ctctggcata
     1141 tgcatcggct gcatgagcag ccccctgctc atgagcacag gttatatgac ggattttatc
     1201 actgtgccgg tacaattcat catatatgct caacaccgct cctccgggat atccgaatac
     1261 ggtgtcacat ccctgctcta tgagtgtttc tattactact tttgttcccg tcgctttcaa
     1321 acttcctcct gtttttaaaa aaatcctaca gttccatgtt ctgtaggatt gtcattttgc
     1381 ttaccggtcg gttatgcggt ccgttccctc cggttgagtt tatatgactc acctgcagca
     1441 ttgctcacaa caactgttgc aataatgact gacagcgccg atactatgat tataatgatg
     1501 cttacggata aatccgtcat tcacaactcc ttaaataatt ttccacaaat gtaccatgag
     1561 cagataaata tgtcaatatt tcagcaccaa ctcccggaaa taaaaaaacc cgattgcaga
     1621 aacttaattc cgcaaacggg gattttaact aactataacg accgtaaaat cattcagata
     1681 ttaattgaga attctgaact tccctataaa cggcaattcc tttgctctac ccttagcggc
     1741 atttataata ccggtaacaa cgaggtatag aagtactaca gcacccgcaa acgatattat
     1801 gccgacaatt atcgccagga atacacgtaa ccaatc
//
LOCUS       DAOPWC010000329         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_69935_length_1835_cov_3.072472, whole genome shotgun sequence.
ACCESSION   DAOPWC010000329 DAOPWC010000000
VERSION     DAOPWC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69935_length_1835_cov_3.072472"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKJ65_06765"
     CDS             <1..307
                     /locus_tag="PKJ65_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41572.1"
                     /translation="KYYLAVSESAVADVLPADKPVLSTTVKYNWNVTDGYLMTPEGMY
                     LVKPSGGYSLSSTSANAVGVYTYDGNLNLITEPVNGIPYLLIANDGANKCVTVNMTQ"
     gene            508..1056
                     /locus_tag="PKJ65_06770"
     CDS             508..1056
                     /locus_tag="PKJ65_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ41573.1"
                     /translation="MKKPDKKTILKIVIPLVIVIAVAGIWFWKNYDRKSDIELNTPLV
                     QPVKEEHFVLNASELDMEAWKSYNLPIVLDFGSDSCLPCVSFYPTLKTVHAEMYGKVI
                     IKYIDVYKYAKEASIYPVQVIPTQIFINADGTPYVPGKNVGVEFITYNFRNTDEHAYT
                     VHEGVLTKDQFFSILRDMGVQE"
     gene            1053..1742
                     /locus_tag="PKJ65_06775"
     CDS             1053..1742
                     /locus_tag="PKJ65_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ41574.1"
                     /translation="MTELLDKIAPLLTEKSWLTPLLALLAGVVTSFMPCSLSSIPLVI
                     GYVGGTGQNDSKRAFKLSLIYVAGTAVTFTALGIIAALAGRLIGTAGSWWYIILGILM
                     VLMALQTWEIFQFIPQSNLLSKSTKKGGIGAFIAGMFAGVFSSPCATPVLVALIAIVA
                     TQNDLLWGALLFILYSIGHGVLSVIAGTSVGFVKKLSTSERYGKASKVISIVMGALIL
                     LMGLYMFYLAF"
BASE COUNT          528 a          323 c          403 g          581 t
ORIGIN      
        1 caaatattat cttgccgtgt ccgaatcggc agttgccgat gtattaccgg cggataaacc
       61 tgtgctgtcc actacggtta agtataattg gaatgtaacg gacggttatc ttatgacacc
      121 tgaagggatg taccttgtca agccttccgg cggatacagt ttatcctcta cctcagctaa
      181 tgcggtgggt gtatacactt atgacggtaa ccttaatctt ataacggagc ctgtaaacgg
      241 tataccgtac ctgcttatag caaatgacgg agcaaataaa tgcgttaccg ttaatatgac
      301 acaataagca attacccgta tccttttgaa tatgctcatg agtaaagctg caaagagtta
      361 atttgcagct ttattcagca gttcgtccgc tataatcgca gtgtttttaa tcacttctat
      421 attcttttct gtgccaatgt cacagtaggt ataatattta ttgtgtatac tctatttgtc
      481 ggttaaattt atgtatggag aaatatgatg aaaaagcctg ataaaaaaac aatattaaaa
      541 atagtaatcc ctttagtcat agttatagcc gtagcgggta tatggttttg gaagaattat
      601 gacaggaaaa gtgatataga gcttaatact cctttagtgc agcctgtcaa agaagaacat
      661 tttgtgctta acgcatcgga actggatatg gaagcatgga aatcatataa cctgcctata
      721 gtattggatt tcggttccga ctcctgtctg ccgtgcgtaa gtttttatcc cacattgaaa
      781 accgtacatg cggaaatgta cggaaaagtt ataataaagt atattgatgt atataaatat
      841 gcaaaggaag caagtatata tccggttcag gttattccta cgcaaatatt cattaatgcc
      901 gacggcacac cctatgttcc tggtaaaaat gtcggagtgg agtttataac atacaatttc
      961 agaaacaccg atgagcatgc atacactgtt catgagggag tgcttacaaa agatcagttt
     1021 ttttccattc ttcgggatat gggagtacag gaatgacgga attactggat aaaatagctc
     1081 cgttgcttac cgaaaaaagc tggctgacac cgttattggc tctgcttgca ggggtagtta
     1141 cttcttttat gccgtgttcg ttatcttcaa tacctctggt gataggttac gtgggaggaa
     1201 cgggtcagaa tgattcaaaa agagctttta aactatcatt gatatatgtc gcaggtaccg
     1261 ctgttacttt tacagcactg ggtattattg ctgctttggc gggcagatta ataggaactg
     1321 ccggttcctg gtggtatatc atactgggta ttctaatggt gcttatggct ttacagacat
     1381 gggagatatt tcaattcata ccgcaaagca atcttttatc caaaagcaca aaaaaaggcg
     1441 gcataggtgc ttttatagcc ggtatgtttg ccggtgtttt ttcctctccc tgtgccacac
     1501 cggtattggt agctttgatt gccatagtgg caacacagaa tgatttactg tggggagcat
     1561 tactcttcat actttactca ataggacacg gagtactgtc ggttattgcc ggtacatcgg
     1621 tgggttttgt taagaaactt tctacaagcg agcgttacgg aaaggcaagt aaagttatat
     1681 ctatagttat gggagcactt atactgctta tgggattgta tatgttctat ctggcatttt
     1741 agtcgcatat agttataatt ttactattta tccgttttct gttgtaaaca gacctgagta
     1801 aatgtgaaat atatggggag agtattatga gcgga
//
LOCUS       DAOPWC010000330         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_70000_length_1834_cov_2.922428, whole genome shotgun sequence.
ACCESSION   DAOPWC010000330 DAOPWC010000000
VERSION     DAOPWC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70000_length_1834_cov_2.922428"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PKJ65_06780"
     CDS             complement(<1..1454)
                     /locus_tag="PKJ65_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ41575.1"
                     /translation="MYKKVSTDLDFVKRELETLEFWKQNRVFEKSIENREGCEHFTFY
                     DGPPTANGKPHIGHILTRVIKDIIPRYKTMKGYKVLRKAGWDTHGLPVELEVEKMLGL
                     DGKDDIERYGIEPFIKKCKESVWKYKGEWEKMSDRVGFWADMENPYITYDNNYIESEW
                     WALKKIWDMGLLYKGHKVVPYCPRCGTSLSSHEVAQGYKDITDKTIYVKFKVKDTADT
                     YLMAWTTTPWTLPSNVALTVGAKFDYAKVKYNNEYIIVAKQLVPSVFEGLDYEIVSTV
                     KGKELEYTKYEPLFDFVDEDAKKEGFYVCLGDFVTLTDGTGIVHTAPAFGEDDSVIGK
                     KYNLPFLQMVDEAGHMAEQTGFIKGLFVKDADKLITEDLDKRGLLFKSSEIEHSYPFC
                     WRCDTPLIYYARSTWFIKMTAIQDSLLKNNDKINWMPATIKDGRFGNFLRAVVDWGIS
                     RERYWGTPLPIWECECGHKEAIGSIAELKEKAVD"
     misc_binding    complement(1500..1705)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1729..>1834)
                     /locus_tag="PKJ65_06785"
     CDS             complement(1729..>1834)
                     /locus_tag="PKJ65_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNZ41576.1"
                     /translation="NELDIPVKYIGIGEKADDLRPFDGKSFIEALFDN"
BASE COUNT          462 a          469 c          314 g          589 t
ORIGIN      
        1 caatcaacag ctttttcctt caactccgct atacttccta ttgcctcttt gtgaccgcat
       61 tcgcattccc atatgggtag aggggtaccc cagtaacgct ctctggatat tccccaatcc
      121 actaccgcgc gtaagaaatt accgaaacgt ccgtccttta tggtagccgg catccagttt
      181 atcttgtcat tatttttaag aagactgtcc tgtattgccg tcatttttat gaaccaggtg
      241 gaacgggcat aataaattaa aggagtgtcg catctccagc agaaaggata ggaatgttcg
      301 atttccgaag acttaaacag cagaccgcgc ttatcgagat cctcggttat caacttatcc
      361 gcatctttaa caaacaagcc ttttataaat cccgtctgtt ccgccatatg tccggcttca
      421 tccaccatct gtaagaaagg caggttgtat ttctttccta taaccgagtc gtcctcaccg
      481 aatgcgggag cggtatgtac aataccggta ccgtctgtca atgtgacgaa atcgcctaaa
      541 catacataaa agccttcttt ctttgcatct tcatcgacaa aatcaaataa aggctcatat
      601 ttagtatatt ccagctcttt tccctttacc gtcgatacta tttcgtaatc caatccttca
      661 aataccgaag ggaccagttg cttggcaacg attatatatt cattgttata cttcactttt
      721 gcgtaatcaa actttgcacc gaccgtaagc gccacatttg acggcaatgt ccagggagtg
      781 gtggtccagg ccataagata tgtatcggca gtatccttta ctttgaattt tacatatatg
      841 gtcttgtcgg ttatatcttt atacccctga gcgacctcat gtgaagaaag actggttccg
      901 catctggggc aatacggcac taccttatgc cctttgtaca gcagacccat gtcccatatc
      961 ttcttcaaag cccaccattc cgattcgata tagttgttgt cataggtaat atatgggttt
     1021 tccatatccg cccagaaacc cacacgatca ctcatctttt cccattcgcc tttatatttc
     1081 cagacgcttt ctttgcactt tttaataaaa ggctcaatgc cgtaacgctc tatatcatct
     1141 tttccgtcca ggcccagcat tttctccact tccagttcta ccggaagccc gtgtgtatcc
     1201 catccggctt tccttaagac tttatatccc ttcatggtct tatatctggg aataatgtct
     1261 tttattactc tggtcaatat gtgaccgatg tgcggttttc cgttagcagt aggcggaccg
     1321 tcatagaatg tgaaatgctc acatccctct ctgttctcta tactcttttc aaagactctg
     1381 ttttgcttcc agaactccaa agtctccaat tcccgcttaa cgaaatccaa atccgttgat
     1441 actttcttat acataacttc ctctttccgg cttagtgcct ataaaaaaac tctctcatcg
     1501 caagggacga aagagtattc gcggtaccac ccaagttgct tttaaagcca cttaattaga
     1561 atgacggtct gctccgggtt accttactgc tgtattcggg taaccggctc cccgatgatt
     1621 ttcaatatgc ttacctgtat gttcgcacct gccacatact ctctttaagg atcggcatat
     1681 ttactcttcg gatccttgct ttttaatata ttataagcat atgccatatt aattgtcaaa
     1741 caatgcttct ataaaacttt ttccgtcaaa cggtcgcaaa tcatcggctt tttcgcctat
     1801 tccgatatat tttaccggaa tatccagttc attg
//
LOCUS       DAOPWC010000331         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_70284_length_1829_cov_3.917700, whole genome shotgun sequence.
ACCESSION   DAOPWC010000331 DAOPWC010000000
VERSION     DAOPWC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70284_length_1829_cov_3.917700"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKJ65_06790"
     CDS             <1..716
                     /locus_tag="PKJ65_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNZ41577.1"
                     /translation="VTAYVAGYFLKIPFKLKSLIGTGTAICGGSAIAALSPVIDADEH
                     DISFSISTVFLFNIAAVFIFPAAGHIFGFSNEGFGLWSGTAINDTSSVVAAAYSFSKE
                     AGDYATVVKLARTLLIVPICLVYAFVFYFNQRRTHNASSSSQTVRYSFIKIFPWFVLG
                     FLFMSMLRSTDIIPIAAADVIAECGKIAIILALSAIGLNADFRRMFKTGIKPVILGLI
                     VWAAVTFSSLAVQYITGKL"
     gene            852..1223
                     /locus_tag="PKJ65_06795"
     CDS             852..1223
                     /locus_tag="PKJ65_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41578.1"
                     /translation="MYKEIKERKWLLVFFTFIAVVAASCGGGNSGDGDVSQLGTKKLL
                     IFYSWPNAINDAISTSDDVSAAAKEYAKYDYVVLNRTFGASDCPQDTVSAIINHPSCK
                     NTIFFGYIDLGMLASSHRLVN"
     gene            complement(1390..>1829)
                     /locus_tag="PKJ65_06800"
     CDS             complement(1390..>1829)
                     /locus_tag="PKJ65_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNZ41579.1"
                     /translation="GPRVAELSDDMLSLKGSAEEISIIDENGNPILSEDEDRRYFEGP
                     WVHKYNGYYYLSYSTGTTHYIAYAISKNPKGPFTYKGRILNPVIGWTTHHSIVEFNGK
                     WYLFYHDSSLSGGKDNKRCVKYTELTYNADGTIQTIDPYTQPV"
BASE COUNT          496 a          324 c          381 g          628 t
ORIGIN      
        1 tcgttacggc ttatgttgcc ggatattttc tgaaaattcc ttttaagctt aaatctctca
       61 tcggaacggg aacagcgata tgcggaggtt ctgctattgc agcactttct cccgtaattg
      121 acgcggatga gcatgatata tctttttcaa tatcgactgt ttttcttttt aatattgcag
      181 cagtttttat tttcccggct gcgggtcata tatttggttt ttcaaatgaa ggattcggtc
      241 tatggtccgg aactgcaata aatgatactt cgtctgtagt agctgcagca tactcgttca
      301 gcaaagaagc gggcgattat gcgactgtgg taaagcttgc acgtactctt cttatagttc
      361 ctatttgtct tgtgtacgct tttgtgtttt attttaatca aagaagaacg cataatgcat
      421 cttcatcttc tcagacagtc agatacagtt ttattaaaat atttccatgg tttgttctcg
      481 gatttctttt tatgtcgatg ttacgaagca cggatataat tccaattgct gcagctgatg
      541 taattgctga atgcggaaag atcgcaatta tacttgctct ttcggcaatc ggacttaacg
      601 cggacttccg ccgtatgttt aaaacaggaa tcaagccggt tattctcggt ctgatagtat
      661 gggcggcagt tacattttcg agtcttgctg ttcagtatat tacagggaag ctataattta
      721 aatctctcaa gcatgccgat taatcgtaat atcttatatt aaattaatca caaaaaataa
      781 cttgataaag gatgagttga tttgatttct gttttagccc gatacatatt tatttagggc
      841 agggggagaa ggtgtataaa gaaataaaag aaagaaaatg gttacttgta ttttttactt
      901 tcattgctgt ggttgcagct tcttgcggag gcggtaatag cggtgatggt gatgtttcac
      961 agcttggaac aaaaaagctt ttgatatttt attcatggcc gaatgcaata aacgatgcca
     1021 tttcgacttc tgatgatgtt tctgctgctg caaaggaata tgcgaaatac gattatgtgg
     1081 ttctgaacag gactttcggc gcaagcgatt gtccccagga tacagtcagc gctataataa
     1141 atcatccgtc atgtaagaat acaatatttt tcggatatat tgatttgggt atgcttgctt
     1201 cttcgcacag gctcgttaat taattctgca ccatttaggg aacggccttc aattaatttc
     1261 ggttcagaat atgcaggcaa aataactgtt aatggaaatc tgattgaacg caaaactaac
     1321 acaggaaagc tttatataaa tactttaaat catgaatatg gattcattga ggattaaaaa
     1381 aagccggtgt taaaccggct gtgtatatgg atctattgtc tggattgttc cgtccgcgtt
     1441 atatgttagt tctgtgtatt ttacacagcg cttgttgtcc tttccgcctg aaagcgagct
     1501 gtcgtgatag aagagatacc atttgccgtt gaactcgaca atcgaatgat gagttgtcca
     1561 tccgattaca ggatttagga ttcttccctt gtaagtgaaa ggccccttcg gatttttgct
     1621 tattgcataa gctatatagt gagttgttcc ggttgaatat gacagataat aatatccgtt
     1681 atacttatga acccatgggc cttcaaaata tcttctgtct tcgtcttctg aaagtatcgg
     1741 gtttccattt tcatcgatta ttgatatttc ttcagctgaa ccttttaatg aaagcatgtc
     1801 gtctgataat tccgcgactc tcggtccaa
//
LOCUS       DAOPWC010000332         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_70287_length_1829_cov_3.793687, whole genome shotgun sequence.
ACCESSION   DAOPWC010000332 DAOPWC010000000
VERSION     DAOPWC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70287_length_1829_cov_3.793687"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKJ65_06805"
     CDS             complement(<1..497)
                     /locus_tag="PKJ65_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ41580.1"
                     /translation="MEREHQIVKAVYAAKENSQKADDLIRDYIPFIRSEASKAVSGFC
                     TEQDDEFSIAMIAFHEAILGYERERGAFLNYASMLIRSRIIDYQRKEARHRGTISLHE
                     ETGEDERTLMDELADEKDYYESSANLEATKQEIEELAATIAQYGVSFSDIADNSPKQG
                     RTLEA"
     gene            806..>1829
                     /locus_tag="PKJ65_06810"
     CDS             806..>1829
                     /locus_tag="PKJ65_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41581.1"
                     /translation="MKHINRVFKSSGKPETRSVRNKGLRLLMLFMVLMLLLTSCTPKV
                     SPRPSEIVTIVPETPTPSETVVPSEEPLPDDPVPEVKTVVIDGITYEVAPLPDMPEQT
                     ELDFAAGKSKYIESNGRYYMDYLSNVRLVSYTNSNAYQGFIEVTGRNAKKEELQFQFY
                     QDTFNRKVESHMGDIVNILWFFLEGDNYSEDKQVYCYGYDLFVDEFVDDFDTSRLSSY
                     ALYTDITPRTLDGVIKYSEMFDDEFRLISVLGNDGKIYDFYLDDSILYDYKKTVGHEV
                     RMNWIIETLLLGNYESPDNERVFLTVRGVMDIELIGQSAYIEELGKSEEGIPYAPSVV
                     IPDDVDL"
BASE COUNT          538 a          349 c          377 g          565 t
ORIGIN      
        1 caggcctcca atgtacgacc ttgttttggc gaattgtccg caatatcgga aaaacttact
       61 ccgtattgtg ctattgtggc tgccaattct tcaatttcct gctttgtggc ttccaggttg
      121 gctgaagatt cataatagtc tttctcatct gccaattcat ccattaatgt tctttcgtct
      181 tcaccggttt cttcgtgcag ggatatcgtg cctctatgtc ttgcctcttt tctttggtag
      241 tcaataatgc gactgcgaat aagcatagac gcataattta aaaaagcacc tctttccctt
      301 tcgtatccca gtatcgcctc gtgaaaagca atcatggcaa tactgaattc atcatcctgc
      361 tcggtacaaa agcctgaaac ggctttggaa gcttccgagc gtatgaaagg gatataatct
      421 cttatgagat catcggcttt ttgagagttt tcttttgccg catagaccgc ttttacaatc
      481 tgatgctctc tttccatacc tcaccctctt ctcgtatata gaatacgcaa aggatttata
      541 taatcggggt cgtatcctat atttttcata aaacccgata aaagtaatat gaattctatc
      601 atatatgttt aaactatgtc ggtaaacagg caattcccga tgtcggggaa aattaatatc
      661 cttacaatta acaaaaagat gatatgtaat gatttgattg agatttgtgt ataaagcttg
      721 ttaaataagt atcactgcta tagttcttat caaattacat gatataatat ccgtgaataa
      781 caccattccc gtggaggtta gttaaatgaa acatataaac cgtgtgttta aatcgtcagg
      841 aaaacctgaa actcgttcgg tgcggaataa aggcttgcgt cttttaatgc tttttatggt
      901 tctgatgttg ctgcttacat cttgtactcc taaggtgtct ccccgtcctt ccgagattgt
      961 aacgattgtt ccggaaactc ctacaccttc ggaaacagtt gtgcccagtg aagagccgtt
     1021 gcctgatgac ccggtacctg aggtgaaaac ggtagtaata gacggaataa catatgaagt
     1081 cgccccgctg cccgatatgc cggaacagac agaactggat ttcgctgcgg gaaaaagcaa
     1141 atacatagaa agcaacggaa gatattatat ggattatctt tccaatgtaa ggctggtatc
     1201 ctacaccaac tccaacgcat atcagggctt tattgaagtt acgggaagga atgcaaaaaa
     1261 agaagaactt caattccagt tttatcagga tacttttaat agaaaagttg aatctcacat
     1321 gggtgatatt gttaatatcc tctggttctt tctggaaggt gataattaca gtgaagacaa
     1381 gcaagtttat tgttacggat atgatttatt tgtagatgaa ttcgtagatg actttgatac
     1441 aagcagactg tcatcgtatg cattgtacac cgacattact ccgcggacat tggacggtgt
     1501 tattaagtac agtgaaatgt ttgacgacga attcaggctc atatccgtac tgggcaatga
     1561 cggtaaaata tatgatttct atttagatga ttccatactg tatgactata aaaaaacggt
     1621 tggccatgaa gtgcgaatga attggattat agaaaccctc ttattgggaa attatgaaag
     1681 cccggataac gagcgggtat ttctcactgt gagaggagtc atggatattg aactcatagg
     1741 acaatcggca tacattgagg aattgggaaa aagtgaggaa ggcatacctt atgctccgtc
     1801 ggttgttata cccgatgatg tagacttaa
//
LOCUS       DAOPWC010000333         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_70600_length_1824_cov_3.302431, whole genome shotgun sequence.
ACCESSION   DAOPWC010000333 DAOPWC010000000
VERSION     DAOPWC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70600_length_1824_cov_3.302431"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKJ65_06815"
     CDS             <1..365
                     /locus_tag="PKJ65_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNZ41582.1"
                     /translation="YVGYRYVASFVVNVPPESFIPAPEVDSTVLKFIKREKPPVDVPS
                     EEFFFSVVRAAFSQRRKKLSNCIAAMPIFDISKNETEDILIQMGVRADARAEALSMEQ
                     FAELSWRLYDRNTNNVNK"
     gene            547..1824
                     /gene="nrdD"
                     /locus_tag="PKJ65_06820"
                     /pseudo
     CDS             547..1824
                     /gene="nrdD"
                     /locus_tag="PKJ65_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816017.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
BASE COUNT          624 a          352 c          407 g          441 t
ORIGIN      
        1 tatacgtggg ttacagatat gttgcttcat tcgtagtgaa tgttccgccg gaaagtttca
       61 ttcccgcacc ggaagtggat agtacggtat tgaagtttat aaaacgggaa aaaccgccgg
      121 tggatgtacc gtcggaagag ttcttctttt cggtggtaag ggcggcattt tcccaaagaa
      181 gaaaaaaact ttccaactgc atagcggcaa tgcctatatt tgatataagc aaaaatgaga
      241 cggaagatat acttatacag atgggtgtaa gggcggatgc cagagccgaa gctctgtcca
      301 tggagcaatt tgccgagctg tcttggagat tatatgaccg aaatacaaac aatgtaaata
      361 aatgacagtt tagcaacact attgcgggat aaaaaaaaca aaatttgttt cgtttaaaaa
      421 ttaacatgta aattcttaaa tttttgtaaa atcgctattg caaaacaaaa agggtagcaa
      481 tatactaata actaactgtt gtgtttatat tcacactaaa catactatat attgggagag
      541 agggtcatga ttacgaaaat tatcaaacgc gatggccggg aggaaagctt ttcgtctttt
      601 agaatcacca gagctatatt cctagccgca gaagcggtag ctaaagaaga aaacaccaaa
      661 gccgatttca caattgcaga acagttgacc gagaaggtcg tgcaattgct caatcgaaga
      721 ttcattgaaa caattcctac agttgaggat gttcaggatg cggtagtaaa agtacttatt
      781 gaagagggtc atgccaaaac ctccgagaag tagattctgt acagagaagc gcgaagtaaa
      841 gcgcggtcca tgaagacacg tctgatgaag gctattgagg aaataacctt tcatgaggca
      901 aatcaaagca atttaaaaag agagaatgcc aacatagacg gcaataccgc aatgggcacc
      961 atgcttcagt acggatctgc cgtatcgaag gaattctgca agaccaatct gttggatccc
     1021 aaacacacca agatgcacga agagggagat atccatattc acgacctgga cttttataac
     1081 atgggaaccc ttacatgttg tcagattgat ctttcccgtc ttttcaaaca cggattttcc
     1141 acaggtcacg gacatcttcg tgaacctcag gatatagcct cgtataccgc actggctgca
     1201 atagcaatac aaagcaatca gaacgatcag cacggcggac aaagcatacc ggcatttgac
     1261 tattatcttg cggacggtgt tgcaaagaca ttcagaagaa cttacagaag caataccata
     1321 aaagccatga agattattta cggcaatgta gcaccggatt gtgatgataa gataaaggag
     1381 ttcgagaaac aggtcggtct gtatgcaaag atagaaatgc acagcgagta tctgaagaaa
     1441 ttcaacgaat tcctggtaaa agaattaaaa gtcaaaacca aagatctgga taagattaat
     1501 aagtttgctt acagtgaatc tttagcggaa accgacaaga gaacatttca ggctatggaa
     1561 ggaatagtac acaatctcaa caccatgcat tcgagagcgg gagcacaggt accgttttcc
     1621 agcatcaact tcggtaccga catatccccc gagggacgta tggtaaccaa gaatcttctg
     1681 cttgctcacg atgcgggctt gggcaacggc gaaacgccga tattccccat actcatattc
     1741 aaggtaaaag aaagtgtaaa ctataatccg gaagatccga attatgattt attcaagctt
     1801 gcatgcagag tttcagcaaa aagg
//
LOCUS       DAOPWC010000334         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_71271_length_1813_cov_2.590444, whole genome shotgun sequence.
ACCESSION   DAOPWC010000334 DAOPWC010000000
VERSION     DAOPWC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71271_length_1813_cov_2.590444"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PKJ65_06825"
     CDS             complement(<1..360)
                     /locus_tag="PKJ65_06825"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ41583.1"
                     /translation="MIKISPSLLACNFARLEEEVRKIEEGGAHYVHVDVMDGHFVPNI
                     SVGFPIVQALKSITKLPLDVHLMISDPDRYIEEFAKAGADIITVQQEACIHLDRTVRY
                     IKEQGKKAAVALNPATDI"
     gene            complement(360..1247)
                     /gene="rsgA"
                     /locus_tag="PKJ65_06830"
     CDS             complement(360..1247)
                     /gene="rsgA"
                     /locus_tag="PKJ65_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ41584.1"
                     /translation="METGLITKALSNRYTVRTDKDEYICKPRGLFRKEGFKPLCGDLV
                     RFDITDHSDKEGYIISIEERKNSLVRPPVANVDVAVIVFATEYPTLDELLIDLLILAF
                     RTNDIEPVLCLNKCDLAAKEDIDDVLRQYSGSGCKIVITDAKHKTGLDPLKSMLSGKV
                     SVFAGQSGAGKSTLINAITGTADMEIGILSGRIGRGKNTTRHCELLSFEGGYIVDTPG
                     FSKFDPGLLQPDLIKGMYNEFLPYIGKCRYNDCRHINDDHCAVLQAVEKGEIPIERHL
                     RYEMIYNMSEEALRVRRGY"
     gene            complement(1248..>1813)
                     /locus_tag="PKJ65_06835"
     CDS             complement(1248..>1813)
                     /locus_tag="PKJ65_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ41585.1"
                     /translation="SETVRVKYGETVKIYVSLGSEFEKTTVPDLFGLTKEQAAGKLLE
                     RNLIMNVMSEEEVPGKASLVSYQYPAADTEIYENSTIDIKLSVDYGDTVYEDITVKYT
                     FDPTIAELAGMEMPITLRFKFEYSDQEEAFQTTAVISSIEKLPYQRDVRIPAKGETKL
                     TIFIGSIIYKEPIIYKYFDHYKETPEP"
BASE COUNT          448 a          445 c          330 g          590 t
ORIGIN      
        1 tatatcggtg gcgggattaa gagcaaccgc ggctttcttg ccctgctctt ttatgtacct
       61 tacggttctg tccagatgaa tgcaggcctc ctgctgaacg gttattatat ccgctcccgc
      121 ttttgcaaat tcttcaatgt atctgtcggg gtcggaaatc ataagatgaa catccagcgg
      181 caattttgtt atgctcttca aagcctgcac aatgggaaag cctactgaaa tattgggaac
      241 gaaatgtccg tccattacat ccacatgcac ataatgggct ccgccctctt ctatttttct
      301 tacttcttct tccaatcttg caaaattaca agccaataat gacggtgaga tttttatcat
      361 caatatcccc tccttactct taaagcttct tcagacatat tatatatcat ttcatacctt
      421 aagtggcgtt caatcggaat ttcgcctttt tccaccgcct gcagtacggc acagtggtca
      481 tcatttatgt gtctgcagtc attgtatctg cactttccga tgtacggcag gaactcatta
      541 tacataccct ttatcaggtc cggttgcaga agaccggggt caaatttgga aaaacccgga
      601 gtatctacga tatagccgcc ttcaaaggaa agcagctcac aatgtcttgt ggtattcttt
      661 ccccttccta tccttccact caaaatcccg atttccatat cggccgttcc tgttatggca
      721 ttgataagtg tggacttccc tgctcccgac tgacctgcaa aaacactgac ttttccgctc
      781 agcatactct ttaacggatc caaacctgtc ttatgttttg catcggttat tactattttg
      841 catccgctcc cggaatattg tcttaataca tcgtcaatat cctcttttgc cgccagatcg
      901 catttgttta aacataatac cggctctata tcgtttgtac gaaatgccag tatcagcaaa
      961 tcaataagca attcatccaa tgtaggatat tccgttgcaa acacaataac cgcaacatcc
     1021 acattggcaa cgggtggtcg taccagactg ttctttcttt cttctatact tatgatatag
     1081 ccttccttat cactatgatc ggttatatca aaacgcacca ggtctccgca aagaggcttg
     1141 aatccctctt tcctgaacaa ccctctcggt ttgcagatat attcatcttt atcggttctt
     1201 acggtataac ggttgctcag cgcttttgtg ataagacctg tttccattta cggttccggg
     1261 gtttctttat agtggtcgaa atatttgtat attatcggtt ctttatagat aatcgagcct
     1321 ataaatatcg tcagtttggt ttcgcctttt gccggtatgc ggacatctct ctgatagggc
     1381 aacttttcaa tggaagaaat aaccgcagtt gtctgaaaag cttcttcctg atcggaatac
     1441 tcgaacttga acctcaaggt tataggcatt tccataccgg caagctccgc tattgtagga
     1501 tcaaatgtgt atttaacggt tatatcctca tacacggtat ctccgtaatc caccgagagc
     1561 tttatatcta tggtgctgtt ttcatatatc tcggtatcgg ctgccgggta ctgataagat
     1621 acaagagaag ccttaccggg cacctcttct tccgacatta cattcattat gaggtttctt
     1681 tccagtaact ttcccgctgc ctgttcttta gtcaatccga acagatccgg caccgttgtc
     1741 ttttcaaatt cggaaccgag agaaacatat attttaaccg tttctccgta tttgactctt
     1801 accgtttccg aag
//
LOCUS       DAOPWC010000335         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_71602_length_1808_cov_2.423274, whole genome shotgun sequence.
ACCESSION   DAOPWC010000335 DAOPWC010000000
VERSION     DAOPWC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71602_length_1808_cov_2.423274"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKJ65_06840"
     CDS             complement(<1..253)
                     /locus_tag="PKJ65_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079912.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNZ41586.1"
                     /translation="MKNKRQVKILEFIKQYPVQTQEELVTLLHNNGFVATQATVSRDI
                     KELKLFKAINKDGLSVYTSNSDVSDNSHEKLIRVLKASFL"
     gene            complement(250..1089)
                     /locus_tag="PKJ65_06845"
     CDS             complement(250..1089)
                     /locus_tag="PKJ65_06845"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNZ41587.1"
                     /translation="MRFSLYANVNKDANGQFLAQVCKYIESIGSVVMHSEIVSKGGKT
                     DISKVMLSDVIITLGGDGTLLDIAQKTKSLSIPLVGINIGSLGFLTQIEKNNYEELIK
                     NITLKQYTISKRAMLSAKVYRNDKLIYDIDALNDVVITRKVTGDTVAISVDINGSYID
                     TYDGDGVIISTPTGSTGYQMSAGGPVSDPEADIMLMTPICPHMMHKQTYILKDDSNLV
                     LTVEEDESFDCCFSYDGIASSLKGRDVIKVKKSSSFIPLLKIQGNSFYENLRNKIYYR
                     GAK"
     gene            1292..>1808
                     /locus_tag="PKJ65_06850"
     CDS             1292..>1808
                     /locus_tag="PKJ65_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNZ41588.1"
                     /translation="MNNEYIKWKSVTLRDNDLNKELIEMNGKPELIEDAFGRHLTFGT
                     GGLRGILGAGTNRMNIYTVRKATFGLAFALESGSSVAIAYDSRIKSDLFAKEAATVLA
                     NCKMKVYLFDRVSPTPLLSFAVRQLKCDAGIVITASHNPSNYNGYKVYNSKGCQINPE
                     TADLILQHINKT"
BASE COUNT          552 a          346 c          316 g          594 t
ORIGIN      
        1 acagaaatga tgctttaagc actcttataa gcttctcatg tgaattatcc gaaacatcgg
       61 agttggaagt ataaacagag agaccgtctt tattgattgc tttgaacagt ttcaattctt
      121 tgatatctct cgaaacggta gcctgtgtcg caacaaaacc gttattatgc aacaatgtga
      181 ccagctcctc ctgagtctga acaggatact gtttaatgaa ctccagtatt tttacctgtc
      241 gtttgttttt cattttgcac ctctgtaata tattttgttt ctgagatttt cataaaatga
      301 attgccctgt attttaagca gcggaataaa agaactactt ttcttaactt ttatcacatc
      361 cctacctttc agtgaggaag caattccgtc gtaactgaag caacagtcaa aactttcatc
      421 ttcttccacc gttaaaacaa ggttgctgtc atctttaagt atgtatgtct gcttgtgcat
      481 catatgagga cagataggag tcataagcat tatatcggcc tcgggatcgc ttaccggacc
      541 tcctgcgctc atctgatatc ctgtggagcc tgtgggtgta cttattataa ctccgtcccc
      601 gtcataagta tctatatatg aaccgtttat atccaccgat atagcaacgg tatctccggt
      661 tacttttctg gttatgacca catcgtttaa ggcatctata tcataaatca atttgtcatt
      721 acgatatact tttgcagaaa gcatcgctct ttttgatatg gtatattgtt tcaaagttat
      781 gttttttata agttcctcat agttattttt ctctatctga gttaaaaagc ccagtgaccc
      841 tatattaata cctaccagag gtattgataa actcttggtt ttctgtgcta tatccagaag
      901 tgtaccgtct ccgcctaaag taatgataac atcggaaagc ataacttttg aaatgtccgt
      961 cttaccgcct ttggaaacaa tctcactgtg cataactacg gaacctatag attcaatata
     1021 tttgcagacc tgtgctaaaa actgtccgtt tgcatcctta ttaacatttg catatagtga
     1081 aaatctcata ttacctgcct tattttatat aaacttctgc tactctttaa atgagtttaa
     1141 ccgattaaat tattaaagca ccttacaata tgtataataa tacattaatc agttaaaaac
     1201 aatgtacaaa catatatttt aagacttttc tataggtaaa atatccatat tactgtataa
     1261 tcagtatgtc gatgaatata gagggtttgc tatgaataac gaatacataa agtggaaatc
     1321 agtaacatta agagataacg atttaaataa agagcttatt gaaatgaacg gtaagcctga
     1381 attaattgag gatgcattcg gcaggcatct taccttcggc acgggcggat tacgcggaat
     1441 tttgggtgca ggcaccaaca gaatgaatat ctacactgta aggaaagcta cattcggatt
     1501 ggcttttgct cttgaaagcg gttcatcggt tgcaattgct tatgattcca gaataaagtc
     1561 ggatttattc gcaaaagaag ccgcaacggt attggccaac tgtaaaatga aagtatatct
     1621 gtttgacaga gtatccccta cccctttatt gtcctttgcg gtaagacaac ttaaatgtga
     1681 tgccggtatt gttataacgg catctcataa tccgtcgaat tacaacggct ataaggtata
     1741 taactccaaa ggctgtcaga ttaatccgga aacggccgat ttaattttgc agcatataaa
     1801 taaaaccg
//
LOCUS       DAOPWC010000336         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_71641_length_1807_cov_3.296233, whole genome shotgun sequence.
ACCESSION   DAOPWC010000336 DAOPWC010000000
VERSION     DAOPWC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71641_length_1807_cov_3.296233"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PKJ65_06855"
     CDS             complement(<1..1795)
                     /locus_tag="PKJ65_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41589.1"
                     /translation="MASVKLQGIYKKYPGGVVAVSDFNLKIKDKEFIILVGPSGCGKT
                     TLLRQLKTVLAPHGSLSGDIIFEGKPLSEVDIRTQSSKIGFVMQSPDNQIVTDKVWHE
                     LAFGLESLGFNTPAIRLKVAEMASFFGIETWFYKNVTELSGGQKQLLNLAAIMAMQPS
                     VLILDEPTSQLDPIAATEFISAVEKINRELGVTVIITEHRLEEVFAISDRVLVMDEGR
                     IICDGTPNEVGEQLKDLHHGMFLAMPVPMRVYAGVENNLACPVTVREGRTWLDTFAKN
                     KQLRDIPAEDSVKEQEGIPVIEFDEVWFKYEKDAPDVLKGLSMKIYQGEFYAILGGNG
                     TGKTTALSLISGINKPYRGTVRINGKELSKIGDKELFNRMLGVLPQNPQALFVKKTVQ
                     EDLLEMLSGQKKSKAEKEQQIAKIAKLCRLEDLLSMHPYDLSGGEQQRAALAKVLLLE
                     PKILLLDEPTKGMDAEFKRIFANILYELLYKGVTVLMVSHDIEFCAKYAHKCALFFDG
                     NIVSWNAPRSFFSDNSFYTTAANRMARHMLPTAITAEDIIYACGGKAEPFEIKIPEDR
                     EIMPIDTKEDTAEEYDISVSSFVSIGMISLSS"
BASE COUNT          479 a          440 c          331 g          557 t
ORIGIN      
        1 cggaagatag ggaaatcatg cctatagata caaaagaaga tacacttatg tcatattcct
       61 ctgctgtatc ttcttttgta tctataggca tgatttccct atcttccggt atctttattt
      121 caaaaggttc cgcttttccc ccgcacgcat aaattatatc ttccgcagtt attgcggtcg
      181 gaagcatatg tcttgccatt ctgttggctg cggtggtata gaaactgttg tcggaaaaga
      241 aacttcgcgg ggcattccaa cttactatat taccgtcaaa gaacagggcg catttatgag
      301 catattttgc acaaaactca atatcatggc ttaccatgag tacggtaacg cctttataca
      361 aaagctcata aagaatattg gcgaatatcc gtttaaattc cgcgtccatg cctttagtag
      421 gctcatctaa aagcaatatc ttaggctcta atagaagaac cttggctaaa gccgcccgct
      481 gttgttctcc gcctgacaaa tcataagggt gcatggataa cagatcttcc aatcgacaga
      541 gtttcgcgat ctttgctatt tgttgctctt tttccgcttt ggacttcttt tgtccggaca
      601 gcatttccaa aagatcctcc tgcacggtct ttttcacaaa aagtgcttga ggattttgcg
      661 gtaatacacc caacatacgg ttaaacagtt ctttatcccc tatcttggac agctctttcc
      721 cgtttattct aaccgttcct ctgtaaggct tgtttatacc ggatatgagc gacagcgccg
      781 tggtttttcc cgtaccgttt ccgcccaata tggcatagaa ctctccttga tatattttca
      841 tggaaagacc tttaagcaca tccggagcat ccttttcata tttgaaccat acttcatcaa
      901 actcaataac gggaataccc tcttgctcct ttactgaatc ctcagcaggg atatctctta
      961 actgtttgtt ctttgcaaag gtatccaacc atgtacgtcc ctctcttacg gtaacgggac
     1021 aagcaagatt attttccaca cccgcataga cacgcatggg aacaggcatt gctaaaaaca
     1081 tgccgtgatg taagtctttc aactgttcac ccacttcatt aggtgtaccg tcacagataa
     1141 ttcgcccttc gtccatcacc aataccctgt cggatatagc gaaaacctcc tccaacctat
     1201 gctcggttat tattacggtg acacctaatt ctctgtttat cttttccaca gcggagataa
     1261 attccgttgc cgctatgggg tccaactgac ttgtgggttc gtccagtata agtaccgacg
     1321 gttgcatcgc catgatagct gcaaggttca gcagttgctt ttgtcctccg gatagttcgg
     1381 taacattttt ataaaaccag gtctctatac cgaaaaatga agccatttca gctactttca
     1441 aacggatagc gggtgtatta aagcctaaac tttccaaccc gaaagccaat tcatgccata
     1501 ccttgtcggt aacaatctga ttatcgggag attgcattac aaaacctatc tttgaacttt
     1561 gggtacgtat atcaacttca gataacggtt tcccctcaaa tatgatatct ccgcttaaac
     1621 ttccatgcgg tgcaagcaca gtttttagtt gtctgagaag tgtggtttta ccgcaccctg
     1681 aaggtccgac aagaataata aactctttat cctttatctt aaggttgaaa tccgaaaccg
     1741 cgacgacacc gcccgggtac tttttataaa taccctgtaa ttttacactt gccattttga
     1801 acctcca
//
LOCUS       DAOPWC010000337         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_71648_length_1807_cov_2.871575, whole genome shotgun sequence.
ACCESSION   DAOPWC010000337 DAOPWC010000000
VERSION     DAOPWC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71648_length_1807_cov_2.871575"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /gene="lepA"
                     /locus_tag="PKJ65_06860"
     CDS             complement(<1..1611)
                     /gene="lepA"
                     /locus_tag="PKJ65_06860"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ41590.1"
                     /translation="MESNLKKDRIRNFCIIAHIDHGKSTLADRLIEKTGLLTSREMSD
                     QVLDNMDIEKERGITIKSQAVRMVYKHNDGKEYILNLIDTPGHVDFSYEVSRSLAACD
                     GAILVVDAAQGVEAQTLANVYMAIDNNLEILPVINKIDLPAARPELAIEEIENLIGIE
                     AHHAPLISAKNRINIEEVLEKIVTYLPAPLDKSDMPARALIFDSKFDAYKGVIIYCRV
                     MEGIIKATDTVRLMNTKKEYQVVELGYLKPGGFIPAEQLTSGEVGYITASIKNVEDTR
                     VGDTVTTAENKAAVPLPGYKEAVSMVFCGIYPADGAKYNMLKDALMKLKLNDAALMYE
                     PETSAALGFGFRCGFLGLLHMEIIQERIEREFDLDIITTAPSVIYKVTKTDGQVLRVD
                     NPTNLPPVSEIAYMEEPIAKAVIIVPAEYVGNIMELCQDRRGTFIHMEYIDATRTKLD
                     YELPLNEIIYDFFDALKSRSKGYASLDYEVMKYEKSKLVKLDILLNQELVDALSFIVH
                     ESKAYARGRRMVEKLKEQIPRHQFEIPVQ"
BASE COUNT          477 a          418 c          304 g          608 t
ORIGIN      
        1 ctgaacgggt atttcaaact gatgtctggg tatctgctct ttaagttttt ccaccattct
       61 tctgccgcgt gcatacgcct tactttcatg cactataaat gaaagagcat ctacaagttc
      121 ctgatttaac aatatgtcca atttgaccag tttactcttc tcatatttca taacttcgta
      181 atccaatgat gcataacctt tggaacggga ttttaacgca tcgaagaaat cgtaaattat
      241 ctcgttaaga ggcaactcat aatcaagttt tgttctcgtg gcatcaatat attccatatg
      301 aataaatgtt cctcgcctgt cctgacacag ctccataata ttacccacat actcggcagg
      361 aactattata acggcttttg caataggttc ttccatatag gcaatttcac ttacaggcgg
      421 aagattggtg ggattatcca ctcgcaacac ttgcccgtcg gtctttgtca ctttgtaaat
      481 aacactgggt gcggtggtga ttatatccag atcaaattct ctttcaattc tttcctgaat
      541 aatctccatg tgaagaagcc ctaagaatcc gcaacgaaat ccgaatccca atgctgccga
      601 tgtctccggc tcatacatca atgccgcatc attgagtttt aatttcatca gagcatcttt
      661 caacatattg tatttggcgc cgtctgcggg ataaattccg caaaatacca tggaaaccgc
      721 ttctttgtaa cccggtaaag gcactgccgc tttattctcc gcagttgtta cggtatcgcc
      781 cactctggta tcttccacat tctttatgct tgcagtaata tatcccactt cgcctgaagt
      841 aagttgctcc gcgggtataa aacctccggg tttcagataa cccaattcca ccacctgata
      901 ctcctttttt gtattcatca gacgaacggt atcggtagcc tttattatcc cttccattac
      961 tctgcagtaa attataacac ctttatatgc atcaaatttc gagtcgaata tgagggctct
     1021 tgcgggcata tcggatttat ctaatggtgc gggaagatat gtcactatct tttctaacac
     1081 ttcttctatg tttatacggt tttttgcgga aataaggggg gcatgatgcg cttcaatccc
     1141 tattaagttc tctatctctt ctatagccaa ttccggtctt gcggccggaa gatcaatttt
     1201 atttattacc ggcagtattt ccagattgtt atcaatagcc atatagacat tggccagtgt
     1261 ctgcgcctcc accccctgag cggcatccac aaccagtatt gcaccgtcac atgcggcaag
     1321 cgatctgctg acttcataag aaaaatccac atgccccggc gtatcgataa ggttaagaat
     1381 atattcttta ccgtcgttat gcttgtatac catacgcacg gcctgggatt taatggttat
     1441 accgcgttcc ttctctatat ccatattatc cagaacctgg tcggacattt ccctgcttgt
     1501 cagtaatccg gttttttcta taagcctgtc tgcaagagtg cttttaccgt gatctatatg
     1561 tgcaattata caaaaattcc ttatcctatc tttcttcaaa ttactctcca tattgatttc
     1621 cagtagagat tatatattaa ctgtcatggc tttacaatca gataattatc tgcgttttta
     1681 tgatatcaat taatgtcttt tttttattta atgaaatatc ataaatgact ttttcaaata
     1741 gcattttttt aatttcctta atactatttt gaggataccc caaagccatt atatcatgtc
     1801 ccgaaat
//
LOCUS       DAOPWC010000338         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_71915_length_1803_cov_2.602975, whole genome shotgun sequence.
ACCESSION   DAOPWC010000338 DAOPWC010000000
VERSION     DAOPWC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71915_length_1803_cov_2.602975"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PKJ65_06865"
     CDS             <1..1250
                     /locus_tag="PKJ65_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ41591.1"
                     /translation="AFKRVDTVKLRTDKDFTCVYDISGERIPGQTVIEDNKKYFLFEA
                     CLCSMGYTIFRLSDEKCIVDDNSFVATCNMMENKHLKVVFDDFMNISSIYDKDAKREV
                     VQKDQTANKITAFEDKPLTYQAWDINIYYNEKPYEINDVTYSALVEDGPVRKCIRVDR
                     KILETTITQYISLECNSRKIDFKTVIDCKHDDLLLKAAFPVDVHTDYATYDIQFGNVR
                     RPTHWNTSWDYARFEVCAHKWADLSEDDHGVSILNDCKYGHDIKDSVIRLTLLKSATE
                     PDPDADRGLHEFTYSLYPHMGDFKKAHTVDMAYKLNFRPYAKYVEKGTGDIENYRSFM
                     TCNHENVIVDTLKKAEEGDGLILRVYECFNRRTECTIDLNFEVSKITECDLMENDIAD
                     AVIEDNVLKFTIRPYEIKTFKIS"
     gene            complement(1237..>1803)
                     /locus_tag="PKJ65_06870"
     CDS             complement(1237..>1803)
                     /locus_tag="PKJ65_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNZ41592.1"
                     /translation="IYSETMSIEDGLNVIKLNDILQCTVCMWHNGVLYVSEFNKYAYD
                     YAAITGITPLLLNTIDLRKCKNGPTKFVFIDTLEKITSIKDMAARLAPSTVTAVTTRP
                     YFLEFFSSSISKATALSKLSEALNIGREQMIAAGDGFNDIDMLDYAGFAVVAGNADEG
                     VKKHADMIACSNNDGVIAYIVDNLIKKS"
BASE COUNT          572 a          346 c          385 g          500 t
ORIGIN      
        1 ttgctttcaa gcgtgtggat accgtaaagt taagaaccga caaggatttt acctgcgtat
       61 acgacataag cggcgaaaga attcccggac aaaccgttat tgaagacaat aaaaaatatt
      121 tcctgtttga agcttgtttg tgctccatgg ggtatacgat attccgtctt tccgatgaaa
      181 agtgcatagt tgatgacaat tcatttgtcg caacatgtaa tatgatggag aataagcatc
      241 ttaaagtggt gtttgacgac tttatgaaca tatcttcaat ttatgacaaa gatgcaaaga
      301 gggaagtggt acagaaagat cagaccgcaa acaaaataac ggcattcgag gacaaacctt
      361 tgacctatca ggcatgggat ataaacatat attacaacga gaaaccttat gagattaatg
      421 atgttacata ctcggcttta gtggaagacg gtccggtaag aaaatgcata agagtggaca
      481 gaaaaatatt ggaaacaact attacacagt atatttcact ggagtgcaat tctcggaaga
      541 ttgattttaa gacggttatt gactgtaaac atgatgattt actgctaaaa gccgctttcc
      601 ccgttgatgt acataccgat tatgccactt atgatataca gttcggtaat gtcaggagac
      661 ctacccactg gaacacaagc tgggattatg ctcgttttga agtatgtgct cacaaatggg
      721 cggatttatc ggaagacgac cacggtgtaa gtattctgaa tgactgcaag tacggtcatg
      781 acataaaaga ttccgttata aggcttacac tgctcaaatc ggcaacagag cccgatcccg
      841 atgccgacag aggattacat gagttcacct attcactcta tcctcacatg ggagatttca
      901 aaaaagccca tacagtggat atggcataca aactcaactt ccgtccttat gcaaagtatg
      961 tggaaaaagg gacgggagac atagaaaatt acagatcctt tatgacatgt aaccatgaga
     1021 atgtcattgt cgatacatta aagaaagccg aagaaggcga cggtctgata ttgagggtgt
     1081 atgagtgctt taacaggcgt accgagtgta caatcgatct caattttgaa gtttccaaaa
     1141 taacggaatg cgacctaatg gaaaacgata tagccgatgc ggtaatagag gacaatgttc
     1201 tcaagtttac cataagaccg tatgagataa agacatttaa gatttcttaa taagattgtc
     1261 tactatatag gctataacac cgtcattgtt ggaacaggca atcatgtcgg catgcttttt
     1321 aactccttca tcggcattac ctgctaccac cgcaaatccg gcataatcca gcatatcgat
     1381 gtcgttaaag ccgtctcccg cagcaatcat ctgctcgcgc cctatattca atgcttcgga
     1441 cagtttggaa agagcggtgg ctttggaaat actgctgctg aaaaactcca gaaaataggg
     1501 gcgtgttgta actgcggtaa cggtggaagg ggcaagcctt gctgccatat ccttaatact
     1561 tgttattttt tccaaagtat ctatgaatac gaactttgta ggtccgtttt tacattttct
     1621 caaatctatt gtgttcagaa gaagaggagt gataccggta atggctgcat aatcatatgc
     1681 atatttgtta aattccgata cgtataacac cccgttatgc cacatgcaca cggtgcattg
     1741 taaaatatca ttgagtttaa tcacattcag gccgtcttca atgctcatgg tctcactgta
     1801 tat
//
LOCUS       DAOPWC010000339         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_72487_length_1794_cov_4.190914, whole genome shotgun sequence.
ACCESSION   DAOPWC010000339 DAOPWC010000000
VERSION     DAOPWC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72487_length_1794_cov_4.190914"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKJ65_06875"
     CDS             complement(<1..591)
                     /locus_tag="PKJ65_06875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNZ41593.1"
                     /translation="MKQFNLAIIGGGPAGMAAALQAYELGVKDIVILERDNSLGGILN
                     QCIHNGFGLHVFKEELTGPEYALRFSEKIKETNITVMLDTMVIGAEKDKTLYAVNPEY
                     GFMVIKAKAVILAMGCRERPRGALRIPGSNCAGVFTAGTAQRFVNIEGILPGKKVVIL
                     GSGDIGLIMARRMTLEGIKVMGVYEIMEYPGGLTRNI"
     gene            complement(587..>1794)
                     /locus_tag="PKJ65_06880"
     CDS             complement(587..>1794)
                     /locus_tag="PKJ65_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ41594.1"
                     /translation="GEEVLQKLYDSGIKNGVTDMAIVDKDFILQREPYVNPLVTKALY
                     AKNAGIVSPYKLNIAAMDNAVMNGVKFLRETKVTGIEKEDGLFHISTEITGDICSISK
                     LDRIKEEDRTDITAKIVINCAGAHADEISRMAGDDSFNIIPRRGEYILFDKTEGSRIN
                     TVLFQTPTEKGKGILVTPTVDGNLMIGPDATVADHKDDKCTNSENLDYVFKTASQTYT
                     RLNIRKSITVFAGIRATPDTSDFIIEESKKCGNFINVAGIESPGLTAAPAIAVYVEDM
                     VKSLMEMKEKAKDQYTMKVKHDKPFSEMTNEEKEAACKSDELCRKIICRCETVSEREI
                     VSAIYAPCGARTVDGVKRRTRAGMGRCQGGFCAPHVMRILSEHTGIAVNKIAKADADS
                     CILGRHSRD"
BASE COUNT          424 a          462 c          320 g          588 t
ORIGIN      
        1 tatattcctg gtaagacctc cgggatattc cattatctca taaacaccca taacttttat
       61 gccttcaaga gtcattcttc ttgccattat gagacctata tccccgcttc ctaaaattac
      121 aaccttttta cccggtaata taccttcgat atttacaaat cgctgtgccg ttcccgcggt
      181 aaacacaccc gcacaattgg aaccgggtat gcgcaatgcc cctctcggcc gctccctgca
      241 tcccatagcc agtattacgg ctttcgcttt tataaccatg aaaccgtact cgggattgac
      301 ggcataaaga gtcttatcct tttccgcccc tataaccatg gtatccaaca taacggtaat
      361 atttgtttct tttatctttt ccgaaaatct caacgcatat tcgggaccgg tcagttcttc
      421 cttgaaaaca tgcagaccga aaccgttatg tatgcactga ttgagtattc cgcctaagga
      481 attgtctctt tccagtataa ctatatcttt taccccaagc tcataggctt gtaatgccgc
      541 agccattccc gccggtcctc cgcctattat cgccaaatta aattgcttaa tcacgtgaat
      601 gcctccctaa tatgcacgaa tcggcatctg cttttgctat tttattcacc gcaattcctg
      661 tatgctcgga aagtatcctc ataacatgag gagcacagaa tcctccctga catcttccca
      721 ttcccgctct ggtacgtctc tttacaccgt ctaccgttct tgctccgcac ggagcataaa
      781 tggctgaaac aatctctctt tccgagacgg tctcgcacct gcatattatc ttcctgcaca
      841 attcatcgga tttacaggcc gcttctttct cttcattggt catttcggaa aaaggcttgt
      901 catgtttaac tttcatggta tattgatctt ttgctttttc cttcatttcc atcagcgatt
      961 taaccatatc ttcaacatat accgctatgg cgggagcggc ggtcagcccc ggagattcta
     1021 taccggcaac attgataaag ttaccgcact ttttactttc ttcaatgata aagtccgaag
     1081 tgtcgggagt ggcgcgtatg cctgcaaaaa ccgtaatgga ttttcttatg ttaagtcggg
     1141 tgtaggtttg cgaagcggtt ttgaatacgt aatccagatt ctcgctgttt gtacatttat
     1201 cgtctttatg gtcggcaacg gtggcatcgg gacctatcat aagattcccg tccacggtgg
     1261 gtgttactaa aattccttta cctttttcgg tgggagtctg gaacagtacg gtatttattc
     1321 tgctgccttc ggttttgtca aacagtatat attcacctct tctgggaata atattaaaag
     1381 aatcatctcc tgccatccga cttatctcat ccgcatgcgc tccggcacag tttatcacta
     1441 ttttcgcagt tatatcagtt ctgtcctctt cttttatcct gtccaattta ctgattgagc
     1501 agatatcgcc ggtaatttct gtggagatat gaaacaatcc gtcttccttt tctatgcctg
     1561 tcactttggt ttcccgaaga aacttaacac cgttcattac ggcattatcc atagccgcta
     1621 tattcagttt atacggcgat actattccgg cattttttgc atacagggct tttgtgacca
     1681 gcggatttac ataaggttct ctttgcagta taaagtcttt gtccacaata gccatatcgg
     1741 taacaccgtt tttgataccg ctgtcataaa gcttctgcag aacctcttct ccct
//
LOCUS       DAOPWC010000340         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_72633_length_1792_cov_2.413356, whole genome shotgun sequence.
ACCESSION   DAOPWC010000340 DAOPWC010000000
VERSION     DAOPWC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72633_length_1792_cov_2.413356"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKJ65_06885"
     CDS             complement(<1..309)
                     /locus_tag="PKJ65_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HNZ41595.1"
                     /translation="MSIKDRLRNTKGFSKGQKKIAEYILANFDKAPFLTATKLAAEAD
                     TSESTVVRFAIELGYDGFPDMQRDMKEVARQTLTSAERLIRISDTVDMDNVLMSVMRS
                     D"
     gene            complement(442..>1792)
                     /locus_tag="PKJ65_06890"
     CDS             complement(442..>1792)
                     /locus_tag="PKJ65_06890"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HNZ41596.1"
                     /translation="SLIATRMSTEDMKDALQLLDKIGYFSLECWGGATFDSCVRFLNE
                     DPWERLRFIRKNVKNTKLQMLLRGQNLLGYKHYADDVVEYFVQKSVANGIDIIRIFDA
                     LNDSRNLETAMKACKKEGGHAQGAICYTISPAHTFEQYLKDAKYLVEAGADSICIKDM
                     AGLLLPYTAYDLVKVLKEHVKLPIQVHTHYTAGTASMTYIKAAEAGADILDCALSPFS
                     QGTSQPPTESIVAALRGTPYDTGLVLADINEISQIFKTVREKYLKSGLLNPKVMGVDI
                     NTLIYQVPGGMLSNLVNQMKEAGKEDQLQDVLKEVPKVREDFGYPPLVTPTSQIVGTQ
                     SVMNVISGERYKMVPKESKDLVAGKYGKTPVAIKQEIIDKILKGEKPITHRPADDIAP
                     ALEETRNEMKEYLEQEEDVLTYAMFPKVAKDFFEHRRAVKYKLDKDLTDMEDKVHPV"
BASE COUNT          445 a          447 c          327 g          573 t
ORIGIN      
        1 atccgatctc ataaccgaca taagcacatt atccatatcc accgtatcgg atatcctgat
       61 aagccgctct gccgaagtga gagtctgccg ggcaacttcc ttcatatctc tttgcatatc
      121 gggaaatccg tcataaccca actcaatggc aaatcgcact accgtggact ccgaggtatc
      181 ggcttccgcc gccagtttgg ttgccgtaag gaaaggagcc ttgtcgaaat tggctaaaat
      241 atattccgct atttttttct gccctttact gaagcctttt gtgtttctca atctgtcttt
      301 aatactcatc gtttcacctg ttttctttat atatcactta ttatgaataa tttaatatcc
      361 gatcctgcat tttttccgtc cgcagggggt gaaaaaacac cttcaggtat ttttgaaacc
      421 taaaggtgtt tgcaattcat tttatacggg atggacctta tcctccatgt cggtaagatc
      481 cttgtccagt ttatacttta ccgcccttct gtgttcaaag aaatcctttg ccactttagg
      541 gaacatggca tatgtaagca catcctcttc ctgttccaga tattccttca tctcgttcct
      601 tgtttcttcc agagccggag caatgtcatc agccggtctg tgagtaatgg gcttttcacc
      661 tttcagtatc ttatctatta tttcctgttt aattgctacg ggagtctttc cgtatttacc
      721 cgctacaagg tctttcgatt ccttgggaac catcttgtat ctttcaccgg aaatgacatt
      781 cattaccgac tgtgttccca ctatctggct tgtaggtgtt acaagcggcg gatatccgaa
      841 atcttctctt accttcggaa cctctttaag cacatcctga agctggtctt ccttacccgc
      901 ttctttcatc tgattaacca gattggaaag cattccgccc ggtacctggt atatgagggt
      961 gttgatgtcc acacccataa ccttgggatt gagcaacccc gatttcagat atttttcacg
     1021 aacggtcttg aatatctgtg atatttcatt aatgtccgct aaaaccagac cggtatcata
     1081 aggtgtacct ctgagtgccg caacaatact ctcggtaggc ggctgactgg ttccctgtga
     1141 aaagggcgat aaagcacagt ctaaaatatc cgctcccgct tcggccgctt taatatatgt
     1201 catggatgcc gttccggcag tatagtgggt atgtacctga ataggaagtt tgacatgctc
     1261 ctttaatact ttaaccaaat cataagccgt atacggaagc aacagtccgg ccatatcttt
     1321 aatacatatg gaatcggcac ctgcttccac aagatatttg gcatctttta aatactgctc
     1381 gaaagtatgc gcaggcgata ttgtgtaaca tattgctcct tgcgcatgcc cgccttcttt
     1441 tttacaggct ttcatggccg tttcgagatt tctcgaatcg ttcaatgcgt cgaatattct
     1501 tataatatca ataccgtttg caaccgactt ctgaacgaaa tattccacaa catcatccgc
     1561 ataatgcttg tatcccaaaa ggttttgtcc tctcaatagc atctgcagtt tggtattctt
     1621 aacatttttc cttataaatc tcagtcgctc ccacggatct tcattcaaaa acctgacgca
     1681 ggaatcaaaa gtggcaccac cccaacactc caatgaaaaa taccctattt tatccagaag
     1741 ctgtaaggcg tctttcatat cttcagtgct catccttgtc gcaattaacg ac
//
LOCUS       DAOPWC010000341         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_73236_length_1782_cov_3.525188, whole genome shotgun sequence.
ACCESSION   DAOPWC010000341 DAOPWC010000000
VERSION     DAOPWC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73236_length_1782_cov_3.525188"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..263
                     /locus_tag="PKJ65_06895"
     CDS             36..263
                     /locus_tag="PKJ65_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41597.1"
                     /translation="MKVLSKIFIILAILLSNVMCAVVAYNYCSLEWGAKYAGYSAPPG
                     AALALAIPYAAGIVILIVLAIIFNRKAGKKS"
     gene            complement(584..1246)
                     /locus_tag="PKJ65_06900"
     CDS             complement(584..1246)
                     /locus_tag="PKJ65_06900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ41598.1"
                     /translation="MTVSKAWDWNKELNSVWLQPSEESYYVAQRWKDKNIKKVLDLGC
                     GLGRHSIFFAKQGFSVSAFDLSLEATEHLKAWSEKENLSIEVVNADMMKLPYKDNFFD
                     AIFAYHVISHTDSAGIREIIKEISRILKIGGEIYITLCSKESASFRDVDNLKIDANTV
                     IKTKEGPEKGIPHFYVEPDDIFSLFDNYGIDRIRHTDDCYFNGKKQNSKHYFISASLR
                     KK"
     gene            complement(1272..>1782)
                     /locus_tag="PKJ65_06905"
     CDS             complement(1272..>1782)
                     /locus_tag="PKJ65_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41599.1"
                     /translation="TLLFSQYETGVLQHGNSNPEDKDYNSLTDFCYKDGNIEIRIPWQ
                     LLNVMDPSTKTVMGNFYKNNSIKGQTIDGFYLGAGVVKEGATDKIDIIMDYYTWSPWS
                     APTYHERLKPSYYILKDAFKQLKLKEKVEVKVNINDYILRKGIHKIPKTTTINDISRL
                     YMYPFCMLK"
BASE COUNT          525 a          355 c          290 g          612 t
ORIGIN      
        1 ggcgagataa ggaacagcga taaggaaggt agcaaatgaa agttttatct aaaatattta
       61 taattctggc aattttactg tcaaacgtta tgtgtgccgt agtcgcatat aactattgct
      121 ctcttgaatg gggagcaaaa tatgccggat acagcgcacc tccgggtgca gccttagcac
      181 ttgctatccc ttatgccgca ggtattgtca tactcatagt attagcgata atctttaaca
      241 gaaaagcagg taagaaatct taatgtaatt tgaaagagta cttggacaaa tacgacgtag
      301 ctctgacatt tcaaacatat tccgtcacag tttttatgac atttcaaaca tattttgcag
      361 cgttttctct gactttttaa aaatactgcc atattctttt ctatgaaatt tatggcatct
      421 atacattttt tttgttcaat agattattag taatagggta caagttgaaa aacactgata
      481 tgacgattac ataagggagt tgcattatag cctccggatg cagttacagt aatcttatat
      541 tgcagcttga atgtgaaaaa ttatttaaac acaaagcaac ctgttatttc tttcttaaag
      601 atgcacttat aaagtaatgt ttactgttct gctttttacc gttaaaatag cagtcatcag
      661 tatgccggat tctatctata ccgtaattgt caaacaaaga aaaaatatca tccggttcca
      721 catagaagtg cggtatgcct ttctccggtc cttcttttgt cttgattacg gtattggcat
      781 ctattttgag attatcgaca tccctgaaag atgccgactc ttttgaacac agtgtaatgt
      841 atatttcacc gccgattttt aatatccttg aaatttcttt aataatttct ctaatccccg
      901 ctgaatcggt atgagaaata acatgatatg caaaaatcgc atcaaaaaag ttgtctttat
      961 acggaagttt catcatatcg gcatttacaa cctcaataga caggttttct ttttccgacc
     1021 atgctttcag gtgctccgtt gcctcaaggg ataaatcaaa agcactgact gaaaacccct
     1081 gttttgcaaa aaatatcgag tgtcttccca gtccgcaacc taaatccaaa accttcttaa
     1141 tattcttatc tttccaacgc tgtgccacat aataactttc ttcactcggt tgcagccaaa
     1201 cggaattcaa ttccttgttc caatcccatg cttttgatac cgtcatgtac aatacctcct
     1261 acctactgca ttcatttcag catacaaaac ggatacatat acagccttga tatatcgttt
     1321 atagttgtcg tcttaggtat cttatgtatg ccttttctta aaatataatc atttatattc
     1381 acttttactt ctactttttc ttttaacttt aattgcttaa aagcatcttt cagaatatag
     1441 taggaaggtt tcagtctttc atggtatgtg ggagccgacc aggggctcca ggtatagtaa
     1501 tccatgatga tatcgatttt gtcggttgca ccttctttta caacacctgc tcctaaatag
     1561 aaaccgtcaa tcgtttgtcc ctttatggaa ttgtttttat aaaaattccc cataacggtc
     1621 tttgttgacg gatccatcac attcagtaac tgccacggaa tccttatttc aatatttccg
     1681 tctttatagc agaaatccgt aagagagtta tagtctttat cttccggatt gctgttaccg
     1741 tgttgtaata ctccggtttc atattgagaa aacagcaacg tt
//
LOCUS       DAOPWC010000342         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_73703_length_1775_cov_3.139535, whole genome shotgun sequence.
ACCESSION   DAOPWC010000342 DAOPWC010000000
VERSION     DAOPWC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73703_length_1775_cov_3.139535"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..329)
                     /locus_tag="PKJ65_06910"
     CDS             complement(33..329)
                     /locus_tag="PKJ65_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41600.1"
                     /translation="MKKTDYDDMTDFEEGILEELALDERDVALLVLIEKEIESTLKNI
                     RIFDLQTNKRMEEILSEEIYNQGEKINRLMDHASPGLKKYLEEYMTKIKETYLN"
     gene            complement(316..993)
                     /locus_tag="PKJ65_06915"
     CDS             complement(316..993)
                     /locus_tag="PKJ65_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41601.1"
                     /translation="MTRRITALILVVIMVFTLTACNNQKTPDGGTPSYVVTTEQNWPS
                     EYLGADVLPKIKGGEVNYYKFYGQILRIYLYNVDDESFMKYIETLKSIGYKNISVETK
                     EAFAGSLDDYYLAMNRDVDTHNMVIELTTPLTWPKEGYITMLSAPPGAKVLQIIGESN
                     NITVVTEDIGKEKFESYTADAIAAGFTAILVTSETVFVAENNDYILTIVRQPDKNTMD
                     IGIYEKN"
     gene            complement(1005..>1775)
                     /locus_tag="PKJ65_06920"
     CDS             complement(1005..>1775)
                     /locus_tag="PKJ65_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HNZ41602.1"
                     /translation="ALNKGYITAGTDVEYVKDIPVYEFNGNIYEKRVYNGYGKPDKNQ
                     ELIYGPNIKAWPEIPRLPKNLLLKAAAVLNDEVTTTDELIPSGETSSYRSNPMALAEY
                     TLSRRAPDYVKKTKLAVRETGVDGHLIFAKKPGDGSAREQAASCQRVLKGYANIAYRY
                     ATKRYRSNLINWGLIPFTVKQDPALTVDEYVYIPDIRDAVLSGKEQVIAYVSATKEDA
                     LQRTDKARAVRLYMEGLSDEERDVLAEGCMINYYRKSM"
BASE COUNT          444 a          430 c          307 g          594 t
ORIGIN      
        1 ctttagtcgg ctgctttaat cttaaggcac aatcagttga gatatgtttc cttaatcttt
       61 gtcatatatt cttcaagata tttcttaagc ccgggagaag catggtccat cagccggttt
      121 attttttctc cctgattata tatttcctcg gataaaatct cctccatacg tttgttggtc
      181 tgtaaatcga atatccgtat atttttcaag gtagactcta tttccttttc tataagcaca
      241 agaagagcca catccctttc gtccaaggca agctcctcca atataccttc ttcaaaatcc
      301 gtcatgtcat cataatcagt ttttttcata tatacctata tccatcgtat tcttatccgg
      361 ttgtcttact atggtaagaa tgtaatcatt attctccgca acaaaaaccg tctccgaagt
      421 caccagtatt gcagtaaacc ctgcagctat cgcatccgcc gtgtatgatt cgaatttctc
      481 tttaccgata tcctcggtaa ctaccgttat attgttggat tcgcctataa tctgtaaaac
      541 cttagcgccg ggaggtgctg acaacattgt gatgtatcct tctttcggcc atgtaagagg
      601 agtggtcagt tcaataacca tattatgcgt atcgacatct ctgttcattg ccagataata
      661 gtcatccaac gatcccgcaa aagcttcttt tgtttccacg cttatattct tatacccgat
      721 acttttcaat gtctctatat acttcataaa ggattcatcg tccacgttat aaagataaat
      781 tctcagtatc tgaccgtaaa acttgtaata atttacttcg ccgcctttaa tcttaggcaa
      841 aacatccgct cccagatatt ccgaaggcca gttctgctcg gtcgttacga cataactcgg
      901 agtacccccg tcaggtgtct tctgattatt gcaagcggtc aatgtaaaga ccatgattac
      961 gaccaatata agtgcagtga ttcttcttgt cattctctcc tccgctacat gcttttcctg
     1021 taatagttta tcatacaacc ttcggcaagt acatcccttt cctcatccga taatccctcc
     1081 atatataacc tgaccgctct tgctttatcg gtcctttgca aagcatcttc ctttgttgcc
     1141 gacacatatg caattacctg ctctttcccc gaaagaacgg cgtctcttat gtcgggaata
     1201 tagacatatt catcaacagt aagagcggga tcctgtttta ccgtaaaagg tataagtccc
     1261 cagtttatga ggttggagcg atatctcttt gtggcatatc ggtatgcgat attggcatat
     1321 cctttcagta ctctctgaca ggaagcagcc tgctcccggg cggaaccgtc tcccggtttt
     1381 ttggcgaata tgagatgacc gtccacaccc gtttccctta cggcaagttt tgttttcttt
     1441 acataatccg gagctcttct ggacaatgta tattccgcaa gagccatggg attggacctg
     1501 taagaggaag tctcccccga aggtataagt tcgtcggtgg tggtaacctc gtcatttaat
     1561 accgcagctg ctttcaataa caaattctta ggcaatctcg gtatttccgg ccacgccttt
     1621 atattgggac cgtatatcag ctcctgattt ttgtcaggct ttccgtatcc gttatatact
     1681 cttttttcat atatgttacc gttaaattca tatacgggaa tatctttaac atattctaca
     1741 tcggtacccg cagttatata tcctttattc aatgc
//
LOCUS       DAOPWC010000343         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_73842_length_1773_cov_3.373108, whole genome shotgun sequence.
ACCESSION   DAOPWC010000343 DAOPWC010000000
VERSION     DAOPWC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73842_length_1773_cov_3.373108"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKJ65_06925"
     CDS             complement(<1..333)
                     /locus_tag="PKJ65_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41603.1"
                     /translation="MEKKRKSSVILIIAMLILLVSCNKNSFDVTTFNSDITIKRSDAQ
                     EPLNIPYRYSKALLDKRTVYSKEITDINISSVAYEISDTGLCLHNYEEVLKDPESDKT
                     AEVIDSIKY"
     gene            complement(360..1715)
                     /gene="radA"
                     /locus_tag="PKJ65_06930"
     CDS             complement(360..1715)
                     /gene="radA"
                     /locus_tag="PKJ65_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNZ41604.1"
                     /translation="MAKNSHYFVCTSCGYETVKWMGKCPSCQEWNTFEEVSEVKSSKT
                     DKVREIELDTVFDIDYNESSRISSGIEEMDRVLGSGIVSGSVILVGGDPGIGKSTILL
                     QLCQHVQTDKDILYISAEESISQLGLRAKRIGLKRSNVKLASETDTDVICKAIIDTKP
                     AVAVVDSIQTVYSDRIPSIAGSVNQIKNSALMLVNAAKRSGTSLFIVGHVTKEGNIAG
                     PKLLEHMVDTVLYFEGESGSSFRILRSVKNRFGSTNEIGVFEMSNTGLTQVTNPSAFM
                     LEGRNMNAPGTVVLPLLEGSRTLMVEIQALVCPTNFSMPRRMASGIDQYRINMLMAVM
                     EKNTGMRLYNYDAYINVAGGIKVNETASDLAVIVAIASSYKGKKAYEQCVVIGEVGLT
                     GEIRSVNGMDKRINEAVKMGYTKAIIPKVNVKAVSQFKDKIEIIGVENVAKAIEASLQ
                     K"
BASE COUNT          443 a          424 c          298 g          608 t
ORIGIN      
        1 gtactttatg gaatctataa cttccgctgt tttgtcgctt tcgggatctt tcaaaacctc
       61 ttcgtagtta tgcagacata aaccggtgtc acttatctca taagcaacgg aagatatatt
      121 gatatctgtt atttctttac tgtataccgt tcttttatcc agtaatgctt tggaatatct
      181 gtatggaata ttcaacggtt cctgggcatc cgacctttta atagtgatgt cgctgttaaa
      241 ggtagtaaca tcaaaactat tcttgttgca agacactaac agtatcaaca ttgcaataat
      301 cagaataaca ctgcttttcc tcttcttttc catactatgt actcccttac tattattctc
      361 tatttctgca acgatgcctc tatagcttta gctacattct caacccctat aatctctatc
      421 ttatccttga attgactgac ggcttttaca ttgactttgg gaatgattgc ttttgtgtaa
      481 cccattttta ccgcttcgtt tatgcgttta tccataccgt ttaccgagcg tatttcgccc
      541 gtaagtccca cttcccctat gaccacacac tgttcatatg cttttttacc tttgtaactg
      601 gaggctattg ccacaataac ggctaaatcg cttgcggttt cgttaacctt tataccgccc
      661 gccacattga tgtatgcatc atagttgtaa agcctcattc cggtgttctt ttccattacc
      721 gccataagca tatttattct gtactgatct attcccgatg ccattcttct cggcatggaa
      781 aaatttgtag gacatacaag tgcctgtatt tccaccataa gagttctgga accttccagt
      841 aaaggtaaca ccacggtacc gggggcattc atattccttc cctccaacat gaatgcagaa
      901 ggattggtca cttgtgttaa accggtattg ctcatttcaa atacgcctat ttcattggtg
      961 gaaccgaatc tgttctttac acttcgcaat atcctgaaag aagaaccgct ttctccttca
     1021 aaatacagta cggtatccac catgtgttcc aaaagcttgg gaccggctat attgccttct
     1081 ttggtcacat gtcctactat aaacagtgat gtacccgagc gttttgccgc atttaccagc
     1141 attaaagcgg aattctttat ctgatttaca ctgcctgcta tggaaggtat ccggtcggaa
     1201 taaacggtct gtatggaatc cacaactgca actgccggtt ttgtatcaat tattgcctta
     1261 catataacat cggtatcggt ctcggaagcc agttttacat tgcttctttt caaacctatt
     1321 ctttttgctc ttaatcccag ctggctgata ctttcttctg cggaaatgta taaaatatct
     1381 ttatctgtct gaacatgctg acatagctga agaagaattg tggactttcc tattccgggg
     1441 tctccgccga ctaaaataac cgagcccgat acaattccgc tgccgagaac tctgtccatt
     1501 tcttctatgc cgctgcttat tctggaagac tcgttgtagt ctatatcaaa aacggtatcc
     1561 aattctattt cccgtacctt atcggttttt gagctcttta cttcacttac ttcctcaaaa
     1621 gtattccatt cctgacatga cggacattta cccatccact tcaccgtttc ataaccgcag
     1681 gatgtgcata cgaaatagtg gctgttcttg gccatagagg ctcctttaaa ttattatttt
     1741 catgagcata ttctttagaa tatccgctct gac
//
LOCUS       DAOPWC010000344         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_74514_length_1763_cov_3.498244, whole genome shotgun sequence.
ACCESSION   DAOPWC010000344 DAOPWC010000000
VERSION     DAOPWC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74514_length_1763_cov_3.498244"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKJ65_06935"
     CDS             <1..359
                     /locus_tag="PKJ65_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41605.1"
                     /translation="GFVFDTLKEYYPGKVLINPTVELYHQYWSDNMIVIVKLTTEAPK
                     GKEIVWHARLEKILVDLFAEPLIMASVSESEYPAIYEDAFTRYVIDKKALFRYANRRK
                     VDKKISEFIRENDIKL"
     gene            658..>1763
                     /locus_tag="PKJ65_06940"
     CDS             658..>1763
                     /locus_tag="PKJ65_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ41606.1"
                     /translation="MDKYMPRIMDELLKRELESFGAVLLTGPKWCGKTTTAKQIAKSV
                     LKMDSPDEQENYLQLADIKPSLLLEGEHPRLIDEWQMAPQLWDAIRHDVDEKSTEGLY
                     ILTGSTSVDETKIRHSGAGRISRLKMYTMSLYESEDSNGSVSLYDLMDNSLNVAAKSD
                     LTIEDYAYLIVRGGWPSTIGKSDTVVRRQIAGYCDAIVKSDISAVDGVSRDERKVSSL
                     LRSYARHTATQAPKTTIQKDVSMNNETMHINTLDSYLNALNRLFVIDDVPAWSPWLRS
                     KTTIRTADTRHFTDPAIAAYFLQAYPQTLLGDMNTFGLLFESLVVRDLRIYAKAHYGH
                     ISHYRDNNGLEADIIIHFPDGRWAAAEVKLGSKQV"
BASE COUNT          570 a          351 c          385 g          457 t
ORIGIN      
        1 cgggatttgt atttgacacg ctgaaagaat attatcccgg taaagtttta ataaatccta
       61 ctgtagagtt atatcatcaa tactggagcg ataatatgat tgttattgta aagcttacga
      121 cggaagcccc taagggaaaa gagattgttt ggcatgcaag attggaaaaa atactggtag
      181 atctgtttgc ggaaccgttg attatggcat ctgtcagcga aagtgagtat cccgcaatat
      241 atgaagatgc tttcacacga tatgttatcg ataagaaagc acttttccgt tatgccaata
      301 ggagaaaagt cgacaaaaaa atatcggagt ttataagaga gaacgatata aagctttaaa
      361 tggagagata gaaattatga ttaaaccgga tagttttaaa gaagggcata taagacagtt
      421 acacatgaaa ttttgttacc tctttgctac ttgaaaagag cttatcccga ggcatatgca
      481 tatgtagtaa agacagatga aataatgata gattgcaaaa aaaacacatt tacaaaaccc
      541 acattaaaac ctctacattt cccacattga aacatgggta aatttttaca aaacccacat
      601 taaaaccttt acatttccca cattaggagt tagaatggtg tcgaggagat aaaaactatg
      661 gataaatata tgccacgtat tatggacgaa ttgctaaagc gtgagctgga atccttcgga
      721 gccgtattac tgaccggccc gaaatggtgc ggcaaaacaa caacggcaaa acaaatagcc
      781 aaaagtgtat tgaaaatgga ttctcccgat gaacaggaaa actacctgca gttggcggat
      841 ataaagcctt ctcttctttt ggaaggggag catcccagac tcatagacga atggcaaatg
      901 gctccgcaac tttgggatgc catacggcat gatgttgacg agaaaagtac cgaaggccta
      961 tatatcctca ccggttcgac atccgtagat gaaactaaaa taaggcactc cggcgcaggg
     1021 cgtatttcca gattaaaaat gtacacgatg agtctttatg agtcggaaga ctctaacggt
     1081 tctgtcagcc tttacgattt gatggataat tcgttgaatg tagcggcaaa atccgactta
     1141 actatagaag actatgcata tctcatagtt cgtggcggat ggccgagtac aataggcaaa
     1201 agcgacaccg tcgtacgaag acagattgca ggatattgcg atgcaatagt aaagtccgac
     1261 atatccgctg tagacggagt ttcccgtgac gaacgtaagg taagttcgct tcttcgttct
     1321 tatgccagac atactgccac tcaagcaccc aaaaccacga tacagaaaga tgtttccatg
     1381 aacaatgaaa ctatgcatat caacactttg gattcgtacc tgaatgcact gaacaggctt
     1441 ttcgttatag acgatgtgcc tgcctggagt ccttggctcc gatcgaaaac gacaattcgc
     1501 acagcagata cccggcactt tacagaccct gctattgctg catattttct gcaagcttac
     1561 ccgcaaactc tgctcggcga tatgaatact ttcggtctgc ttttcgaatc tcttgttgtc
     1621 agagacctgc gaatctatgc taaagctcat tacggccata tttctcatta tcgggacaac
     1681 aacggactgg aagcggatat tattatccat ttccctgacg ggcggtgggc ggctgcggaa
     1741 gtaaaactgg gaagtaagca ggt
//
LOCUS       DAOPWC010000345         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_74661_length_1761_cov_3.729191, whole genome shotgun sequence.
ACCESSION   DAOPWC010000345 DAOPWC010000000
VERSION     DAOPWC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74661_length_1761_cov_3.729191"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1761)
                     /locus_tag="PKJ65_06945"
     CDS             complement(<1..>1761)
                     /locus_tag="PKJ65_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNZ41607.1"
                     /translation="EEDIADDASKELLRIRSSILKNQEAIKDKLLSFTGSDKYRKYMQ
                     DNIITMRQDRYVIPIKSEYRNVVKGVIHDTSQTGSTLFIEPMEIVNMNNKIRELINDE
                     KKEIERILRKLSLQCADYKEEILSNFNTCCYLDVMFAKAAYAISYSGTIPGINKNREI
                     RLLNAKHPLIPEDIVVPIDFKIGKDFRILVITGPNTGGKTVTLKTVGLLTAMAHAGLA
                     IPADEGSEICVFNSIYADIGDEQSIEQNLSTFSSHMTNIINILKNADDLSLVLLDELG
                     AGTDPVEGSALASAIIETLRNRNITTVATTHYTELKTYASVTENVENASCEFDVKTLK
                     PTFRLLIGIPGKSNAIYISRRLGLDEEIIKMAKSSIDNKSSDYENVILSLEKSRQRME
                     KDRIKATEYKKEIEDIKKQLEKERDGIGKEREKILEKANKDAKKLVEKALDKAQHLIN
                     EIDQFKKSNITVNAKTEADFRTTVRKTQEILQNKETDEERKTDITADDISSGDTVMLR
                     NLKQKADVLTNPDKDGNIIVQAGIIKMTVNIKDVYKIDEQKEINKNLKMNVIKASGPA
                     KMELDIRGNTIFEAESKLDKF"
BASE COUNT          417 a          383 c          259 g          702 t
ORIGIN      
        1 aaacttatcc agcttggatt ccgcttcaaa aatcgtattc ccgcgtatat ccaattccat
       61 ctttgcgggt ccggaagctt ttattacatt catttttaag tttttattta tttccttttg
      121 ttcatctatt ttatatacat cctttatatt aacggtcatt ttaatgattc ccgcctgaac
      181 aataatattt ccgtctttat cgggattggt taaaacgtcc gctttttgct tcagatttct
      241 taacattacg gtatcaccgc tacttatatc atcagcagtt atatctgttt ttctttcttc
      301 atccgtttct ttattctgca gtatttcctg cgtttttctg acagtagttc tgaaatccgc
      361 ttccgttttg gcatttaccg tgatattgct ctttttaaat tgatctatct catttataag
      421 atgctgtgct ttatctaaag ccttttcaac aagctttttt gcatctttat ttgctttttc
      481 aagtattttc tcacgctctt taccgatgcc gtccctctct ttttccagct gttttttaat
      541 atcttctatc tcttttttgt attcagtcgc tttaattcta tctttttcca ttctttggcg
      601 gcttttctcc agtgaaagaa taacattttc ataatcgctg cttttgttgt ctattgaact
      661 tttcgccatc ttaattattt cttcatccaa tcccagtctt ctggatatat atatggcatt
      721 actttttcca ggtattccta taagcaacct gaatgtcggc tttaatgttt ttacgtcaaa
      781 ctcacagctt gcattctcca cattttccgt aaccgaagca tatgtcttca attccgtgta
      841 atgtgttgtt gcgaccgttg ttatatttct gtttcttaat gtttctatta tagctgatgc
      901 aagcgcggaa ccttctacgg gatcggttcc cgcacccagt tcatccaaca ataccagact
      961 caaatcatcg gcatttttca gtatgttaat aatattagtc atatgtgatg aaaatgtact
     1021 taagttctgc tctatgctct gttcatcacc tatatctgca taaatgctgt taaaaacaca
     1081 tatttcgctg ccttcatcgg caggaattgc caggccggca tgtgccattg ctgttaaaag
     1141 gcctaccgtc ttaagtgtaa cggtttttcc tccggtattc ggacctgtaa ttacaagtat
     1201 tctgaagtct ttgcctatct taaaatctat cggaactaca atatcctcgg gtataagcgg
     1261 gtgttttgca tttaaaagac gaatttccct gttcttattt ataccgggaa tggtgccgct
     1321 gtatgatatt gcatatgcgg cttttgcaaa catgacatcc agataacaac aagtattgaa
     1381 attactcagt atctcttctt tatagtccgc acattgcaag gaaagctttc ttaatatcct
     1441 ctcaatctct ttcttttcat catttataag ttcccgtatc ttattattca tattcactat
     1501 ttccataggt tctataaaca aagtggaacc tgtttgtgat gtatcatgaa taacgccttt
     1561 tacaacattt cggtattcac ttttgatagg aatgacatat ctgtcctgac gcatagtgat
     1621 tatattgtcc tgcatatact ttctgtactt atcacttccc gtaaaagaca gcagtttatc
     1681 ctttatagct tcctgattct ttaatatgct gcttcttatt cgcagcaatt cctttgatgc
     1741 gtcgtccgca atatcctctt c
//
LOCUS       DAOPWC010000346         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_76171_length_1737_cov_3.335315, whole genome shotgun sequence.
ACCESSION   DAOPWC010000346 DAOPWC010000000
VERSION     DAOPWC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76171_length_1737_cov_3.335315"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKJ65_06950"
     CDS             complement(<1..849)
                     /locus_tag="PKJ65_06950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNZ41608.1"
                     /translation="MVFSGLFFLTVFLPACLILYYISKNRTYRNIVLTVMSLVFYAWG
                     EPVYILLLLLTSAFVYIVALLIQQKAGTRSSKIYLITGITVIIAILVFFKYSGFIAQN
                     LSAIFKFKNPFNDIALPLGLSFYTFTCISYLADVYKKKTEAQRNFSDLLMYISVFFTV
                     TSGPIRRYPDWVADIRSDRETWEKFNYGVHRFIIGLAKKAIIANTAGTLISSFTGTDY
                     SRLSVQGAWIGILLFSLQIYFDFSGYSDMAIGLASMFGFSVNENFNYPYISKSATEFW
                     RRWHITL"
     gene            complement(849..1568)
                     /locus_tag="PKJ65_06955"
     CDS             complement(849..1568)
                     /locus_tag="PKJ65_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HNZ41609.1"
                     /translation="MSGLVISQMRKAIIDYDMIQDEDTIAVGLSGGKDSLALLYGLSK
                     IRGHIEQSFDIKAITLKLSEENPDTDYLKKFCDSIDVELVVKQTDIEQIVFEERKEKS
                     PCSLCANLRRGMLNDTAKTLGCNKVALGHNKDDAIETFLLCLQYEGRLHTFAPVTYLS
                     RKDITVIRPLIYSFEQDIMAFNESAGITPVKTLCPAAGFTHRQRIKEYIENESSINPN
                     FKYNLFRSFTHANISGWKEVL"
BASE COUNT          514 a          353 c          326 g          544 t
ORIGIN      
        1 cagtgttata tgccatcttc tccagaactc cgtagcgctt ttagatatat acgggtaatt
       61 gaagttctca tttaccgaaa agccgaacat ggaagccaat cctatagcca tatcggaata
      121 accggaaaaa tcaaaatata tctgtaacga gaacagaagt attcctatcc atgcgccttg
      181 taccgacagt ctggagtaat cggtacctgt gaaagaagat atcaaagtgc ctgcggtgtt
      241 ggctatgatt gctttcttgg ccaaccctat aataaatctg tgcactccgt aattgaattt
      301 ttcccaagtt tctctgtcgc ttcttatatc cgctacccag tcgggatatc ttcttatagg
      361 tcccgatgtc acggtaaaaa acaccgatat gtacataagc aagtccgaaa agtttctttg
      421 ggcctcggtt ttctttttgt aaacatccgc aagataactt atgcatgtga aagtatagaa
      481 agacaagccc aaaggtaagg caatgtcgtt gaaaggattt ttaaatttga atattgccga
      541 taagttttgc gcaataaatc ccgaatactt gaagaatacc agtatcgcta taattaccgt
      601 tatacctgtt ataaggtaga ttttcgatga acgcgtgccg gctttttgct gtataaggag
      661 ggctacaatg tatacgaaag ccgaagtcag cagtaaaagc agtatatata ccggttctcc
      721 ccatgcataa aatactaaag acatgacggt aagaacgata tttctatatg ttcggttttt
      781 ggaaatgtag taaagtatga ggcatgcggg caggaaaacc gttaaaaaga aaaggccgga
      841 aaaaaccatc agagtacctc cttccatccg gaaatattgg catgcgtgaa tgaccggaaa
      901 aggttgtatt taaagttggg attgatggaa ctttcgtttt caatatattc ctttattctc
      961 tgacgatggg taaagcctgc tgcggggcac agtgttttaa ccggtgtaat acccgcactt
     1021 tcattaaaag ccattatgtc ttgttcaaaa gaatatataa gcggtcttat caccgttata
     1081 tcttttcttg aaagataggt aacgggagca aaagtatgca gtcttccttc atattgtaaa
     1141 catagcagaa aagtttctat ggcatcgtct ttgttgtgac cgagagccac tttattacaa
     1201 cctaatgttt ttgccgtatc attcaacata cctctgcgta aattggcaca taaagagcag
     1261 ggactttttt ctttccgttc ttcaaaaaca atctgttcta tatcggtctg ctttacaacc
     1321 aattccacat cgatgctatc gcagaatttt ttaagataat cggtatcggg attttcttcg
     1381 ctgagtttta aggtaattgc ttttatatca aacgattgct ctatatgccc tctgattttg
     1441 cttaatccgt aaagcagagc aagactgtct tttcctcccg acagtcccac tgcgatagta
     1501 tcctcatcct gaatcatatc gtaatcaatt atcgcttttc gcatctggct tataactaaa
     1561 ccgctcatta ttcgaataat accattttag tgtatgaatt tcaattatat gtaatccctg
     1621 ccggtcagat atctgtaaag catcatatac cccattcccg ttctcggtat atccgacatt
     1681 acctcataat agctgccgtc ttttaaggaa aacagtattc tcatcatttt tgtaacg
//
LOCUS       DAOPWC010000347         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_77320_length_1721_cov_2.731092, whole genome shotgun sequence.
ACCESSION   DAOPWC010000347 DAOPWC010000000
VERSION     DAOPWC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77320_length_1721_cov_2.731092"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PKJ65_06960"
     CDS             <1..1076
                     /locus_tag="PKJ65_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ41610.1"
                     /translation="PIVSVLGIIGTSLAIWQGGVMILGEVITVGTLTLFISYTLQFFL
                     PVNDMARIFARFQFSQAAGERIMALLEADIDIKDSDEVIEKYGNITSDMPVPKLKGSI
                     RFENVSFRYKEGEKVLDTFNLNVEPGQCIALVGETGSGKTTIVNLACRFYEPTEGTIY
                     IDGQDYKTIPLMYIHKNLGYMLQTPHLFSGTIMDNIRYGREDATEEEVINAAKMVEAH
                     DFIMKFENGYNHVISKGGGGISTGQKQLICIARAIVNDPCIFVLDEATSSVDTQTEKA
                     VQRAISKVLYNRTSFVVAHRLSTIREADRILVIDKGKVIEQGSHEELMKLKGHYHNLY
                     TSQFIDDSNKDLFGTPEPLPVED"
     gene            complement(1371..>1721)
                     /locus_tag="PKJ65_06965"
     CDS             complement(1371..>1721)
                     /locus_tag="PKJ65_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41611.1"
                     /translation="IKTGYAVKYAVMDAACDFYPYSEDGKVRHETWTTPILAFYYTDK
                     PNRKADISKVMDKKIDSIKCHKSQVDSNLLMFVKELAKIAAKGTKFKYAEQFRALSSL
                     QMHCFNIALEEDND"
BASE COUNT          542 a          313 c          386 g          480 t
ORIGIN      
        1 tcccgatagt atcggtattg ggtatcatag gaacctcgtt ggccatatgg cagggcggag
       61 taatgatttt aggcgaagtg ataacggtcg gaacgcttac actgttcata tcgtacactt
      121 tacagttttt ccttcctgta aacgatatgg caagaatatt tgccagattc cagttttctc
      181 aggctgcggg tgaacgaatt atggctttgc tggaggcgga tatagacata aaggattccg
      241 atgaggttat tgaaaaatac ggaaacatta catcggacat gccggtacct aaactgaaag
      301 gttccataag atttgagaat gtgtctttcc gatataaaga aggcgagaag gttttggaca
      361 ctttcaactt aaacgtggaa ccgggtcagt gcatagctct ggtaggagaa accggatcgg
      421 gaaaaacaac gatagttaac ctcgcatgca gattctatga acctaccgag gggacaatat
      481 atatcgacgg gcaagactat aaaacgatac ctttgatgta tatccacaag aacttaggct
      541 acatgctgca gactccgcat cttttcagcg ggacaattat ggacaatatc cgttacggca
      601 gggaagacgc aacggaggaa gaggtcatta atgctgccaa gatggtggaa gcccatgatt
      661 tcataatgaa attcgaaaac gggtacaatc atgttatctc caaaggcggc ggcggaatat
      721 ccacgggaca gaaacagctt atctgcattg caagagcaat agtgaatgat ccctgtatat
      781 ttgtccttga cgaagccaca agttctgtgg atacgcagac cgagaaagcg gtgcaacgtg
      841 ccatttccaa ggtgttatat aacagaacaa gttttgtagt tgcacacaga ctgtcgacaa
      901 taagggaagc cgatagaata ttggtaattg ataagggaaa agtaatagaa caggggagtc
      961 atgaagagct tatgaagctg aaaggacact accataacct ttacaccagc cagtttatag
     1021 acgacagcaa taaggattta ttcggtactc ccgaaccgct acccgtagag gattaataac
     1081 aaacaacaac gcaccttaac aggtgcgtgt ttgttttaat caaatgaagc ggttttatga
     1141 acttaataaa ggtgagaaaa aaaagtgtca ccgccggtgt cacaattgag tcggacgaat
     1201 tattaagacg atggaatagg ttttacttcc ggttggaaaa tcaaaattat tagcataata
     1261 tgaattagga taatgtaaat tatgctaatt tgatagaaaa agataagaag attataggat
     1321 attcttttgt aaaggatttt tagctattaa taagtacttg ttttttctaa tcagtcgtta
     1381 tcttcctcta aagcaatatt aaagcagtgc atctgcagtg aagataatgc tctgaactgt
     1441 tccgcatact tgaattttgt tccttttgcc gctatcttgg caagttcctt tacaaacatc
     1501 agaagattgg agtcaacctg ggatttatga catttaatcg aatcaatctt tttgtccata
     1561 acctttgata tatccgcttt tctgttgggt ttatcggtgt agtaaaatgc aagtatcggt
     1621 gttgtccatg tttcatgacg caccttaccg tcttcggaat agggataaaa atcacatgcg
     1681 gcatccatga ccgcatattt taccgcgtaa cctgtcttta t
//
LOCUS       DAOPWC010000348         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_77453_length_1719_cov_3.743990, whole genome shotgun sequence.
ACCESSION   DAOPWC010000348 DAOPWC010000000
VERSION     DAOPWC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77453_length_1719_cov_3.743990"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..>1719)
                     /locus_tag="PKJ65_06970"
     CDS             complement(307..>1719)
                     /locus_tag="PKJ65_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41612.1"
                     /translation="KIFRFIVILFILLSMLFSYTGCQEKVKESNIPIETAGTETAVVP
                     VESDTVSTPDPEVTEEEQTDSQEEVVTDNTGTEVPGKPSRTYIDNMIFVNGKSLPVFA
                     LPGVFRSLDGNLVRKPTNQDFKNLQASRDPVTHEITGSLPNEVLSVDGEEPVNADSLV
                     IYAGPYDYEFKYTYIEKDGRRQPVFSHGTDLQLTRIPMTNKYAVVKGGFLFWVDLDEE
                     TMDLYCLSETMGYSMYDNFWEIDGIKYLKVWAIRPSFNRDGTKMLFYTERATEEVGYV
                     WVKDTVTGEEKPVPNTPAFTRVLQWRENRYAYLQSFGKIIEIDTVELTAKTVFDNEVT
                     GSNSFNMIYPYVFNSVLDKDRNIFSEIINLNDGSKRKFEDSKYQRCDNITVVPNTNLL
                     LLRYAYPQENNPIYHEAVILDLVTGKRCVISVPFEYNISSIYQYREYYVVNLYKYGDI
                     NNVHDQTSYFIPRTEIRFTD"
BASE COUNT          515 a          355 c          266 g          583 t
ORIGIN      
        1 aaaagtcatt ctgtgacggt gccgcggcaa aaagcatatt aatttcatct tttgttttaa
       61 ataatctcaa tatctcactg cgtacatcct gagcggcttt taagcccatc tctcttcttg
      121 tatcaaatac cgatacttcc aaattatcct ttttgaaatt cttaattaac ataacaactc
      181 ctttaatttg atatatctat tatatctcaa atcaaaaaag gcacaatcgg aatagtgcct
      241 tttcaagata aaacaaaaaa caggattgaa aatataaaat aaatttataa atcactaaag
      301 ataatgctaa tctgtaaaac gtatctcggt tctcggtata aaatatgatg tctggtcatg
      361 tacattgttt atatcaccgt atttatacag attaactaca tagtattccc tgtattgata
      421 aattgaagat atattatact caaaaggaac cgatatcaca catcttttac ctgtaactaa
      481 atccagaatt accgcttcat gatatatcgg attgttctct tgcgggtagg catatcttaa
      541 aagcagaaga tttgtgttgg gaacgacggt tatattatca cacctttgat atttcgaatc
      601 ctcaaatttc cttttacttc cgtcattcag attaataatc tcggagaaga tatttctgtc
      661 tttatctaaa accgaattga aaacataggg ataaatcata ttaaatgaat tacttccggt
      721 tacttcgtta tcaaatacgg tcttggctgt aagttcaaca gtatcaatct caattatttt
      781 tccgaagctt tgtaaatatg catacctgtt ttctctccat tgcagtactc ttgtaaatgc
      841 cggcgtattc ggaacaggtt tttcttcacc ggtaacggta tcttttaccc atacataacc
      901 tacttcttcc gtagctcttt cagtatagaa taacattttt gttccgtctc tgttgaagga
      961 aggtcttatc gcccacactt tcagatactt aattccgtct atctcccaaa aattatcgta
     1021 catggaatat cccatagttt cgctgagaca atataggtcc attgtttcct catccaaatc
     1081 cacccagaat aaaaagccgc ctttcaccac agcatattta tttgtcatcg gaattcttgt
     1141 taactgtaaa tcggttccat gagaaaaaac cggttgtctt cgaccgtcct tttcaatgta
     1201 tgtatatttg aattcataat catacggtcc tgcataaata accaatgaat cggcatttac
     1261 aggctcttct ccgtcaaccg acaacacttc attgggcaaa cttcccgtta tttcatgcgt
     1321 tacgggatct cttgaagcct gtaagttttt aaaatcttgg tttgtaggtt tcctgaccag
     1381 attgccgtca agtgagcgga atactcccgg taatgcaaat acgggtaggc ttttaccgtt
     1441 tacgaaaatc atattatcga tatatgttct tgaaggttta cctggtactt cggtaccggt
     1501 attatccgtt acaacttcct cctgtgagtc tgtttgctct tcttcggtta cttcaggatc
     1561 aggggtactt accgtatccg actcaacggg aacaactgcg gtttccgtac cggccgtctc
     1621 tatcggaata ttgctttctt taaccttttc ctgacaacct gtataagaaa ataacatgga
     1681 caacaggata aataaaataa cgataaatct aaatatttt
//
LOCUS       DAOPWC010000349         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_77527_length_1718_cov_3.091401, whole genome shotgun sequence.
ACCESSION   DAOPWC010000349 DAOPWC010000000
VERSION     DAOPWC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77527_length_1718_cov_3.091401"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1482)
                     /gene="mtaB"
                     /locus_tag="PKJ65_06975"
     CDS             complement(163..1482)
                     /gene="mtaB"
                     /locus_tag="PKJ65_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNZ41613.1"
                     /translation="MKTYSIVTLGCKVNQYESATIASTLDAMGFQYKKFPDICDIYVI
                     NTCTVTAMSDRKSRQMIGKARASNTDSIIIATGCSAQADAQALHGSGADIVLGNNEKS
                     CIDTILSSYLKEKTGKLTVVSDIFREKEYIHGCFPDKWERTRGYLKIQDGCDNFCSYC
                     IIPYVRGRARSRDIDDIVAEARYMASKGIKEVVLTGIHLDSYGKDKGNLSIIDVISKI
                     NDIEGIERIRLGSLEPKIITETFVEEIKKYDKLCPHFHMSLQSGSDAVLSDMRRRYDS
                     EMFLNAINMLRNNIPDVEFTTDIIAGYPTETEEDFNKSLEMVEKARFIKVHVFKFSKR
                     PGTTAAKLKPVDGKILNGRSEKLINYASEISKSRLSGYLGKTLRILIEESEDGFITGH
                     TANYIKVQAPSDDNTADDCINTFCDVLICGLTDDGLMGEVVAKKTVR"
     gene            complement(1490..>1718)
                     /locus_tag="PKJ65_06980"
     CDS             complement(1490..>1718)
                     /locus_tag="PKJ65_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ41614.1"
                     /translation="LLPAAIDLVVEAGQASVSYLQRKLKLGYGRAARIVDQMELRGIV
                     GPFEGSKPRAVLISRDEWAEMQMNNPDLDIN"
BASE COUNT          434 a          397 c          297 g          590 t
ORIGIN      
        1 tgtttctgca tgatgacctt tcagaaagtc gagattaatt gttgatcctc tatccacttt
       61 acatatctcc tttgcagttc atacttgtag tttactgtaa aacattgttt ttttcaagaa
      121 taaatatgta aattcaatct atagtttttt taattgaccg ctttatcgga cagttttttt
      181 tgcaacaacc tcacccataa gtccgtcatc cgtcaagccg caaatcaata catcgcaaaa
      241 tgtattgata cagtcgtctg cagtattgtc atccgacgga gcctgcactt ttatatagtt
      301 tgccgtatga ccggtaatga aaccgtcttc gctctcttca ataagtatcc gtaatgtctt
      361 tccgagatac cctgaaagtc ttgattttga tatttccgat gcataattta ttaacttttc
      421 gcttctcccg ttaagaattt tgccgtcaac aggtttaagt ttagccgctg ttgtaccggg
      481 tcgtttggag aatttaaaga catgcacttt gataaaccgg gctttttcca ccatttccag
      541 actcttatta aagtcctctt ccgtttcggt cggatatccc gctattatgt cggtggtaaa
      601 ctccacatcg ggaatgttgt ttctcaacat gtttattgca tttaaaaaca tttccgaatc
      661 atatcgcctt ctcatatcgg ataaaactgc atccgaaccc gattgcaatg acatatgaaa
      721 atgggggcat aatttgtcgt atttctttat ttcttccaca aaagtttccg ttattatttt
      781 gggctccaga gaacctaacc ttattctttc aataccctct atgtcattta tctttgaaat
      841 gacgtcaata atgctcagat tacctttgtc tttgccgtaa gagtccaaat gtatgcctgt
      901 aagtacaact tctttaatgc ccttggatgc catgtatcgg gcttccgcaa cgatatcgtc
      961 aatatccctg cttctggctc ttcctcttac gtaaggtata atgcagtagg aacaaaaatt
     1021 atcacatccg tcctgtattt taagataccc tctggttcgc tcccatttat cgggaaagca
     1081 accgtgtata tattcttttt cacggaaaat atccgaaaca accgttaatt taccggtttt
     1141 ttcctttaaa tatgatgaaa gtatcgtatc aatgcagctt ttttcattat ttcccagtac
     1201 tatatccgca ccgcttccgt gcagggcctg tgcatcggct tgagccgaac atccggtagc
     1261 gataatgatg ctgtctgtat ttgacgctcg cgctttacct atcatctgcc gtgattttct
     1321 atcgctcata gcggtcacgg tacatgtgtt tataacatag atatcgcaaa tatcgggaaa
     1381 ctttttgtat tgaaaaccca ttgcatcaag agttgatgca atggtcgccg attcatattg
     1441 atttactttg catcccaagg ttactattga atatgtcttc atcttatttt cagttgatat
     1501 ccaaatcggg attattcatc tgcatttccg cccactcgtc tctggagata agtaccgctc
     1561 tgggcttgga accttcaaaa ggtccgacta tacctcttaa ctccatctgg tccacaattc
     1621 ttgcggctct gccgtacccc agtttcagtt ttctctgcag ataggaaacc gatgcctgac
     1681 ctgcttccac cacaaggtct attgccgcag gcaacagt
//
LOCUS       DAOPWC010000350         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_78182_length_1709_cov_2.523579, whole genome shotgun sequence.
ACCESSION   DAOPWC010000350 DAOPWC010000000
VERSION     DAOPWC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78182_length_1709_cov_2.523579"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..885)
                     /locus_tag="PKJ65_06985"
     CDS             complement(115..885)
                     /locus_tag="PKJ65_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNZ41615.1"
                     /translation="MKKVAIVTGAARGIGNGIAYQLATEGYDIAILDVFAQEDVTENV
                     DRVIKAGAELLYFKGDLAKTEDRLDFVKEVMNKYGRIDVLVNNAGVGPKVRMDILQTT
                     EESFDFVMNINVRGTYFLTQAVANEMIKCKETLKDCNPKIVNISSMSAYTSSTSRGEY
                     CISKAGVSMITKLFADRLAEYEINVYEIRPGIIYTPMTEVVRAKYDKLIAEGISPIKR
                     WGYPEDIASVVSAFVSGKFPFSTGDVINVDGGLHIQRL"
     gene            complement(952..>1709)
                     /locus_tag="PKJ65_06990"
     CDS             complement(952..>1709)
                     /locus_tag="PKJ65_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNZ41616.1"
                     /translation="LLAYKAGFDGVDMKACHRYLNSGLLGAFTREGKYGGSFENRIRF
                     MVNCVDKIRVAVNDDNFIISTRINIYDSIPYPYGFGVDKCNYLLPDYDEPVKLAKILY
                     SKGVRLLNVTMGTPYYNPHINRPFDSGEYIPHEHPLEGVARLISGAAKIKKQVPKMVV
                     MGTGYSWLRNFAPYVAAYVKENNMADIIGFGRVSLAYPDFADDILHNDGMIANKCCIC
                     CGKCVELMRAGSTPGCVIKDGEVYLPLYRELNK"
BASE COUNT          456 a          426 c          279 g          548 t
ORIGIN      
        1 agcattaaat cccgcagcac cggttcctac tattaccgtg tttaccgaga taagatcata
       61 ttcaatcttg tttatagtta ttgtactttt tttcatcatt ccctcctgaa gtttctataa
      121 cctctgtata tgcaagcctc cgtccacatt tatgacatcc cccgtcgaaa acgggaattt
      181 acccgatacg aatgccgaaa cgacagatgc gatgtcttcg gggtatcccc atctcttaat
      241 gggagaaata ccttccgcta tcagtttgtc gtatttggct cttaccacct cggtcatagg
      301 tgtataaatg attcccggcc ttatctcgta tacattgatt tcatattccg ccagtctgtc
      361 tgcaaaaagt ttggttatca tgctgacacc ggctttggat atacaatact ctcctctgga
      421 tgtcgaactg gtatatgccg acatggagct tatatttact attttagggt tgcaatcttt
      481 caaagtctct ttacacttaa tcatttcatt tgccaccgcc tgagtaagaa aatatgtgcc
      541 tctgacatta atattcatta cgaaatcaaa gctctcttcg gttgtttgca agatatccat
      601 acgtactttg ggacctactc ccgcattatt taccagaaca tcaatcctgc cgtatttatt
      661 cattacctct tttacgaaat ccagtctgtc ttccgtcttt gcaagatcgc ctttgaagta
      721 taagagttcg gcacccgctt ttataactct gtctacattc tccgtgacat cctcctgtgc
      781 aaatacatcc aaaatggcta tatcatatcc ttcggtagcc aactgatacg ctataccgtt
      841 tcctattcct cttgccgctc ctgttacaat tgcaactttt ttcatctttc cattacctcc
      901 aatcactttg ctttatatca cttcaattct gaaactaata tttccattaa gttatttatt
      961 caactccctg tataacggca gatacacttc cccgtcttta ataacacatc cgggagtgct
     1021 gcccgctctc ataagctcca cacattttcc gcagcagata cagcatttat tggcaatcat
     1081 accgtcattg tgtaaaatat catcggcaaa atccggataa gccaaagaaa ctctgccgaa
     1141 tcctataata tccgccatat tattctcttt aacataagcg gcaacatacg gagcgaagtt
     1201 ccttaaccag gaatagcccg ttcccatgac aaccattttc ggtacctgct ttttaatctt
     1261 agcggcaccc gaaatgagcc ttgccactcc ttctaaagga tgttcgtgcg gtatatattc
     1321 acccgaatcg aaaggtcggt tgatgtgggg gttatagtaa ggagtaccca tcgttacatt
     1381 cagcaatctt actccttttg aatagagaat cttcgccagt tttacaggct catcgtaatc
     1441 aggcagaaga taattgcatt tatccacacc gaatccgtaa ggatacggaa tggaatcgta
     1501 gatatttatg cgtgtggaaa taatgaaatt gtcgtcattt acggccactc ttatcttatc
     1561 tacacaatta accataaatc tgatacggtt ttcaaagctt ccgccgtact ttccttctct
     1621 tgtaaatgcc cctaaaagac cggaattgag atatctgtga catgctttca tatccacacc
     1681 gtcaaagccc gctttatacg ccaataagg
//
LOCUS       DAOPWC010000351         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_78453_length_1705_cov_3.283636, whole genome shotgun sequence.
ACCESSION   DAOPWC010000351 DAOPWC010000000
VERSION     DAOPWC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78453_length_1705_cov_3.283636"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PKJ65_06995"
     CDS             <1..1074
                     /locus_tag="PKJ65_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ41617.1"
                     /translation="AEKNDLLGQAYAMRALGYFYAVRVWGDVPLYTEPNEKYSEDIYK
                     ERTDKDYILRNVVLEDLKIAEGLMDRVKNLERKRISIAGVWAIMSDVYMWLEDYNMAD
                     QTISKIQNFKVKNNTVQFIGLEKDIATWRKVFVEELNGKASDDTPENDEYTTKELIFV
                     IHFSMDEVGADGYSYMYQWFTGSGNRAAVLSNKFMSILHEDDKRKDYIAMNYQNGWEL
                     NKFIGGTISTTLNKTCVVAYPVYRYSDILLLQAEARVKMGKWEEALDIVKQVRDRAGL
                     VTPTAMEFGSEEDIIDFILEERQIELVGEGRRWFDLLRTNRWRKVMEPINGMTEEGNQ
                     YFPIHYSHINENHLIKQNSFYGN"
     gene            1100..1702
                     /locus_tag="PKJ65_07000"
     CDS             1100..1702
                     /locus_tag="PKJ65_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNZ41618.1"
                     /translation="MKKNSFYIMIVLGLMTYSCSLELQTDYDYKASTIDPHIPMTAWE
                     YFNTRTDLFSIFIEAVEYTELKNYYTQTDEKYTFLALTNMAMTSYMQDRFPGVLSISE
                     CNKESVKQLLLYHLVQGEYSAYKQLDVEPMYVLTMLKGEEGLMTMLTRKNPWQADAGK
                     VIVNDTGSNGNSYMRAAVTSNIMPTNGVIHIFDAFCYYRK"
BASE COUNT          575 a          264 c          385 g          481 t
ORIGIN      
        1 gcagagaaaa atgatttgct cggacaggct tatgccatgc gggctttggg ttatttttat
       61 gctgttcggg tatggggtga tgttccgtta tacacggaac caaatgaaaa gtattctgag
      121 gatatctaca aagaaagaac ggataaagat tacatcttgc gaaatgtagt attggaagat
      181 ttaaagattg ccgagggttt gatggatcgt gtaaaaaacc tggaaagaaa gcgtatttcg
      241 atagccggcg tgtgggctat tatgtcggat gtttatatgt ggctggaaga ttataacatg
      301 gcggatcaaa ctatttcgaa aatacagaat tttaaagtta agaataacac ggtacagttt
      361 ataggccttg aaaaagatat tgccacctgg agaaaagtat ttgttgaaga actgaatggt
      421 aaggcttctg atgatacgcc cgagaatgat gaatatacaa cgaaagaatt aatatttgtc
      481 attcacttca gcatggatga agtaggtgca gacggttata gctatatgta tcagtggttc
      541 acaggttccg gaaaccgggc agcagtattg tccaataaat ttatgagtat attacatgag
      601 gacgataaac ggaaggacta tatagccatg aactatcaaa acggctggga actgaataaa
      661 tttatcggag gaaccatcag taccacactc aacaaaacct gcgttgtcgc ttatcctgtt
      721 tacagatatt cagatatatt gctgttacaa gcagaggcgc gtgttaaaat gggtaagtgg
      781 gaagaagctt tggatatcgt caaacaagtt cgcgatagag cggggttagt tactcccaca
      841 gccatggagt ttggctcgga agaagatatt attgatttta ttttggaaga acgccagatt
      901 gaattggtcg gagaaggtcg tcgttggttc gatttactaa gaaccaaccg gtggagaaaa
      961 gtcatggaac ccatcaatgg catgactgaa gaaggaaacc agtatttccc cattcattac
     1021 tcacacatca atgagaatca tctcatcaaa caaaattcgt tttatgggaa ttaaattaga
     1081 aaaacaaaat tataatcata tgaaaaaaaa tagtttttat atcatgattg tattgggttt
     1141 gatgacctat agctgttcgc tcgaattgca gacagactat gattataaag catcaacgat
     1201 agacccacat attcctatga cagcttggga gtacttcaat acacgaacag acttgttctc
     1261 aatttttata gaagctgtgg aatataccga attgaagaat tattataccc aaacggatga
     1321 aaaatatacc tttttagcgt tgacgaatat ggctatgaca agctatatgc aagatagatt
     1381 tccgggagta ttatccattt cggaatgtaa taaagagtca gtaaaacaat tacttcttta
     1441 tcacttggtt caaggagaat atagtgcgta taagcagtta gatgtggaac ccatgtatgt
     1501 gctaaccatg ttgaaaggag aagaaggact tatgactatg ttgacacgta aaaatccttg
     1561 gcaggctgat gccggaaaag ttattgtgaa tgatacgggc agcaatggaa actcatatat
     1621 gcgggctgca gtaacaagta atattatgcc cacaaatgga gtaatacata tttttgacgc
     1681 cttttgttat taccgtaaat aacag
//
LOCUS       DAOPWC010000352         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_79722_length_1688_cov_2.381506, whole genome shotgun sequence.
ACCESSION   DAOPWC010000352 DAOPWC010000000
VERSION     DAOPWC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79722_length_1688_cov_2.381506"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PKJ65_07005"
     CDS             <1..1219
                     /locus_tag="PKJ65_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ41619.1"
                     /translation="ARQVGKTWLMKEFGSNYYDNYVYFNFDEEDQLKSIFEANKNPHR
                     IVELLSLIAGKKILPQETLIIFDEIQECSEALNTLKYFKEKANEYHVIAAGSLLGTLL
                     AKPKSYPVGMVNVLNIFPMCFEEFLEATDPMLFTYYESIEKKQEIFDIFHNRLIDAYN
                     YYLIIGGMPECVSSWVKYKDPAGISKIQRELIEIYENDFSKHNGNVNSGRILQVFRSI
                     VAQLSKPNEKFMYGTVRKGGRARDFEEAIEWLVSAGMLNRVYNVSKMKHPLAAFDKLE
                     QFKLFVFDTGLLKHMAGIDNSAILLSSDFQFKGPLTENYILQQLRGQFEVEPRYYSDK
                     NNELDFVLQYGTAIIPVEVKGGTDKSAPSFKKYISEHKPEYAIRYSKRGYKKDGYITN
                     IPLYLAGKTKELL"
     gene            1295..>1688
                     /locus_tag="PKJ65_07010"
     CDS             1295..>1688
                     /locus_tag="PKJ65_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41620.1"
                     /translation="MSKVRIGIFGVRRGSSVVRYCRTQPDAQLVAICDKWEQGLMDKK
                     KELSEDIAYYTSFDDFIKHDMDAVVLANYANEHAPYAIHCMERGLHVLSEVLPCQTMA
                     EAVQLVETVEKTGKVYAYAENYCFMAAPY"
BASE COUNT          532 a          288 c          377 g          491 t
ORIGIN      
        1 tgcacgacag gtcggcaaga catggcttat gaaggagttc ggaagtaact actacgataa
       61 ctatgtttat ttcaactttg atgaagaaga tcaattgaaa tctatttttg aggcaaataa
      121 aaatccccat cgtattgttg agcttctttc cctgattgcg ggtaaaaaaa tacttccgca
      181 agaaacactg attattttcg acgaaattca ggaatgctcg gaggctttga atacacttaa
      241 gtacttcaag gagaaagcaa atgaatatca cgttattgct gcgggaagtc ttttgggtac
      301 tcttcttgct aaaccgaaat catatccggt cggtatggtc aatgtactca atatttttcc
      361 tatgtgtttt gaagaatttc ttgaagcaac cgatcctatg ctttttacat actacgaaag
      421 tattgagaag aaacaggaga tttttgatat attccataat cgcttaatag atgcttataa
      481 ttattacctc attatagggg gaatgccgga atgcgtctca tcgtgggtta aatacaaaga
      541 ccctgccgga atatcaaaga tacagcgcga attgatagag atttatgaaa atgacttttc
      601 taaacacaac ggtaatgtaa acagcgggcg tatactgcag gttttccgta gtattgtggc
      661 acaactttcc aaacccaacg aaaagtttat gtatggaact gtcagaaaag ggggtagggc
      721 acgggacttt gaagaagcta ttgaatggct ggtttcagca ggtatgctca accgggttta
      781 caatgtttca aaaatgaaac atcctttggc tgcatttgat aaacttgagc agtttaagct
      841 gtttgtcttt gatacgggat tgcttaaaca catggcaggt attgataaca gtgccattct
      901 tttaagcagc gattttcagt ttaaaggacc gctgaccgaa aactatattt tacagcaact
      961 tcgcggtcaa tttgaggtag aacccagata ctactccgat aagaataatg agttggattt
     1021 tgttctacag tatggcactg caattattcc ggttgaagta aaaggcggaa cggataaatc
     1081 cgcaccttca ttcaagaaat atatttccga gcataaacct gaatatgcta tccgttattc
     1141 caagcgcgga tataaaaagg acggttatat aaccaatata ccgttatacc ttgcaggaaa
     1201 aacgaaagaa ttgctctgat tcagtacatt tgcgttttat atatatttta tatagtataa
     1261 ttcagtcata gtataaaaaa gaggtgacat gtatatgagt aaggtaagaa taggaatctt
     1321 cggtgtaaga agaggttcct ctgttgtgag atactgccgg acacagcccg atgcgcaact
     1381 ggtggcaatc tgtgataaat gggaacaggg tcttatggat aagaaaaagg agctgagtga
     1441 ggatattgcc tactatacct cctttgacga ctttatcaag catgatatgg atgcggtggt
     1501 gcttgccaac tatgcaaacg agcatgcacc gtatgcaata cattgtatgg aaagaggact
     1561 gcatgtatta agtgaggtat tgccttgtca gacaatggct gaggcggtgc aacttgtgga
     1621 aacggtggaa aaaaccggta aagtctatgc atatgcggag aattactgtt ttatggctgc
     1681 accttacg
//
LOCUS       DAOPWC010000353         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_79778_length_1687_cov_3.068627, whole genome shotgun sequence.
ACCESSION   DAOPWC010000353 DAOPWC010000000
VERSION     DAOPWC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79778_length_1687_cov_3.068627"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1365)
                     /locus_tag="PKJ65_07015"
     CDS             complement(61..1365)
                     /locus_tag="PKJ65_07015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ41621.1"
                     /translation="MKYYSRALIIVIILATIFVIGMAIEFDHSNIIIKSDTLKPFQEM
                     RVSNGINRCEYKAKVILNIDFVMPQTLLFKTSHLRFNIYINEKMVYSFGYEENAVSFL
                     KSPGTSYHLVELPAMSSNKEIVFDFQSPYSNYHGFLPDIQYGTKGECIAYYQNKSMYS
                     LFSLTVVLLGGLTIMILYFVTKNKKYALSKSFLYISSFIFLVSIWLFFQSDSCQLMFG
                     YPQIMYFADLISLVLFPVPLNLYIYDVSKNEKRKYMLYFAYAYAATLVLNLLLQVFGL
                     VDMFQLVLLTHSLMALNMIVAVYVVHVELRIEKNKDLRMFIVPLALVSAGGLLEMILY
                     YYSNMTQTSFALRIAVVAFLVVIIINAIKSYYDNLLELKQAEYYEQLASVDMLTNLGN
                     RNKYEQFLQSHEPNQPLTAVLFDLNYLKYINDNFGHVVGDSA"
     gene            complement(1495..>1687)
                     /locus_tag="PKJ65_07020"
     CDS             complement(1495..>1687)
                     /locus_tag="PKJ65_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ41622.1"
                     /translation="KSVYKITPNRDLIIARIEKAGTLLQTKDYSVNQVAEMLGYSSVY
                     HFIRQFKSVTGVTPGSVRK"
BASE COUNT          553 a          347 c          286 g          501 t
ORIGIN      
        1 tattctgaaa cactcgcctt tattggaaaa agcttctttt atgcattccg cacacttttt
       61 ctatgcactg tcccctacca catgtccgaa gttatcattt atgtacttca gatagttcag
      121 atcaaaaagt accgcagtca aaggttgatt aggttcatga gactgcagaa actgttcgta
      181 tttattcctg ttgcccagat ttgtcaacat gtccacactt gccaactgtt cgtaatattc
      241 cgcctgtttc aattccaaca aattatcgta ataactcttt atcgcgttaa taattatcac
      301 aaccaaaaat gcaacaaccg caatacgtaa cgcgaaggat gtctgtgtca tattggagta
      361 atagtaaaga atcatctcaa gaagaccgcc tgcagatacc agcgccagag gtacaatgaa
      421 catgcgaaga tccttgtttt tctcaatacg caactctaca tgtacgacat ataccgcgac
      481 aatcatattc aaagccataa gcgaatgagt aagaagcacc agctggaaca tatccacaag
      541 accgaatacc tgaagcagta agtttaaaac caatgttgcg gcataggcat aagcaaaata
      601 tagcatgtac tttctctttt catttttgga aacatcatat atgtataagt tcaaaggaac
      661 cggaaagagc acaagactta tgaggtcggc aaaatacatt atctgcggat atccgaacat
      721 aagctggcag gaatcgcttt ggaaaaacaa ccaaatcgat accaggaata tgaaactgct
      781 tatatacaga aaggattttg acagtgcata cttcttgttc tttgtaacga aatacaaaat
      841 cattatagtc aatccgccca acagcacaac agtcagactg aacaacgaat acatggattt
      901 attctggtag tatgctatac attcaccttt tgtaccgtac tgtatatcag gcaggaaacc
      961 gtggtaattg gagtaaggac tctggaaatc gaaaacaatc tctttattgc tgctcatagc
     1021 cggaagttcc accaggtgat aggaggttcc gggagatttc agaaaagaaa cggcattttc
     1081 ttcatatccg aaagaataga ccattttttc gttaatatat atattaaaac gcaaatgact
     1141 ggtcttgaac aacagagtct gcggcatgac gaaatcaata ttaagtatta cttttgcttt
     1201 gtattcacac cgattgattc cgttcgacac tctcatttcc tgaaagggct tgagagtatc
     1261 actcttaata attatattgg aatggtcaaa ttctatcgcc attcctatta caaatattgt
     1321 agctaatata atgacaatga ttaatgctct tgaataatat ttcacgtcat accttccaat
     1381 aaaaaactac caagtttata aaacagtata ttattgatac ttcgtattgt aaacaccttt
     1441 tttcaaaaaa ccgtatattt aacacaaaaa gtccattatt ttaaactttt tttatcactt
     1501 tctcacgctt cccggagtaa ctcccgttac cgacttaaac tgccggataa agtgataaac
     1561 actgctgtaa cctaacatct cggcaacttg gtttacggaa taatccttag tctgcaaaag
     1621 agtcccggct ttttcgatac gtgcgataat gaggtctctg ttgggtgtaa tcttgtacac
     1681 cgatttg
//
LOCUS       DAOPWC010000354         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_79788_length_1687_cov_2.707721, whole genome shotgun sequence.
ACCESSION   DAOPWC010000354 DAOPWC010000000
VERSION     DAOPWC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79788_length_1687_cov_2.707721"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..912)
                     /gene="recR"
                     /locus_tag="PKJ65_07025"
     CDS             complement(316..912)
                     /gene="recR"
                     /locus_tag="PKJ65_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ41623.1"
                     /translation="MADIYPKPLSRLIAAFEMFPGIGRKMATRLAFHLLTMDDEKIQY
                     ISESIRDAKAGLSYCSVCCNITQKDPCEICSSSRDHSVICVMSSVKDVMAMERTCEYD
                     GVYHVLHGIISPMEGVNPEDLKIKELLKRLDGNVKEIIIATNSSVEGEATAMYLSRLI
                     KPFNIKVTRIAHGISVGTEIEYADEATLIRALKGRTAI"
     gene            complement(913..1228)
                     /locus_tag="PKJ65_07030"
                     /pseudo
     CDS             complement(913..1228)
                     /locus_tag="PKJ65_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018933893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
     gene            complement(1258..>1687)
                     /locus_tag="PKJ65_07035"
     CDS             complement(1258..>1687)
                     /locus_tag="PKJ65_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41624.1"
                     /translation="KVQPKVQVKDKPVKKEVQKPEAVKPAVNETVKAVKTETKSDQNI
                     SWSKYIQDRFDKGSKDAFELLQSAIEISGNQAVVTVANDFLKSSLERNGVAVLEEIRG
                     FSKDITNVRFVVAGTDKVAGSPEQQITEIGKKHNIPVEII"
BASE COUNT          411 a          416 c          284 g          576 t
ORIGIN      
        1 attgttttaa taatctatat ttcttcacaa aaaacaaccc tcttgtcttt atttccgacc
       61 tgagggtttt acgcataaaa catatatgtc ataaagattc cacaggtcac cgtttcgcct
      121 gcagaatgcc gttcaactat tctgaacaag ccacaggcat acctatcccg tcattcaatg
      181 ctactgtatt tctgagtttg atcatcgtat gccccttcct ttcaagttaa tcttttttga
      241 ttatatacaa catattttac atagtcaatg caattttgtt gcagttgtgt aacaattcaa
      301 acaacatttc gtaaatcata tagcggttct ccctttcagt gctcttatca atgttgcctc
      361 atcggcatat tctatctcgg tacctaccga aataccgtga gcaattcttg tgacttttat
      421 gttgaagggc tttataaggc gggaaagata cattgcggta gcctctcctt ccaccgagga
      481 attggtcgct ataataattt cttttacatt gccgtccaac ctttttaaca gttcctttat
      541 ctttaaatct tcgggattta caccttccat aggtgatatt attccgtgta atacatggta
      601 cacaccgtca tactcgcagg ttctttccat ggccataaca tcttttaccg aagacattac
      661 gcaaataacc gaatggtctc ttgaggaaga acatatttca caaggatcct tctgcgtaat
      721 attgcagcag acggaacaat aggaaagacc ggctttggcg tctcttatgg attccgatat
      781 gtactgaatt ttttcatcat ccatggtaag aagatgaaaa gccaaacggg tcgccatttt
      841 cctgcctatc cccgggaaca tttcaaacgc agctattaat cgtgataaag gtttcggata
      901 tatatcagcc atttagaaag gcatcctcat gccgccggtc gctttaccca tagtttcctc
      961 ttttttggca tttatctttc ctaccgactc gttaactgca gtcaaaatta aatcctgaag
     1021 catctccaca tccgcatctt ccaaaatagc ttgatcaata gttatggagt ccattgtata
     1081 gtcctttcat agcaactgta actaccccgc ctccggcttt ggactcgaat gtgctgtttt
     1141 ccaattcttc ctgtgccttg gcaatgtcgg cctgcatctt ctgcgcctgt ttgagcatac
     1201 tgctatagtt catatttccg ccgcccattc ttccgcgtgc catattgtct acctccgtta
     1261 tataatctct acgggtatat tgtgtttctt cccgatttcc gttatttgct gttccggtga
     1321 gccggcaact ttgtcggtgc cggcaaccac gaaccttaca tttgttatat ccttactgaa
     1381 tcctctaatc tcttccaaca ccgcaactcc gtttctttcc aaactgcttt ttaaaaaatc
     1441 atttgctacg gttacaaccg cctggtttcc gcttatttca atggccgatt gtaataactc
     1501 gaacgcatct ttggaaccct tatcaaacct gtcctgtata tatttggacc aggatatatt
     1561 ctgatcggat tttgtttccg tcttaaccgc cttaacagtt tcgttgacgg ccggtttgac
     1621 ggcctccggc ttttgcactt cctttttaac aggcttatcc ttaacctgca ctttaggttg
     1681 tactttt
//
LOCUS       DAOPWC010000355         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_80380_length_1679_cov_3.351601, whole genome shotgun sequence.
ACCESSION   DAOPWC010000355 DAOPWC010000000
VERSION     DAOPWC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80380_length_1679_cov_3.351601"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKJ65_07040"
     CDS             <1..269
                     /locus_tag="PKJ65_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ41625.1"
                     /translation="KLYHRKKYPESLYLLAMLDYVSRVNNIPLCTKYKALRKVKLEET
                     LYPAGILAMAVISGQSKIKEDALSQSIPEFLRHNIIESEVRNVI"
     gene            259..1026
                     /locus_tag="PKJ65_07045"
     CDS             259..1026
                     /locus_tag="PKJ65_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNZ41626.1"
                     /translation="MSSDTVHAFNKDNLDTYLKALAKLFRKQNGKRATAEIILVGGAA
                     VLVNYGFRDMTADIDAIIHASASIKDAINQIGDEYNLPNGWLNTDFMHTASYSEKLNE
                     FSVYYKTFYGILQVRSISAEYLIAMKCKSGRKYKNDMSDIIGILAEHKKREEPLTWEK
                     VNQAVINLYQDWNGIAKEIKDFIRNAVMTDNYEEVLKQIKEQEQLTKNILIQFQKEHP
                     HTVTESNVDSILRMLESKKIADNIEGKNRICTEDEML"
     gene            complement(1053..>1679)
                     /gene="pnuC"
                     /locus_tag="PKJ65_07050"
     CDS             complement(1053..>1679)
                     /gene="pnuC"
                     /locus_tag="PKJ65_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNZ41627.1"
                     /translation="IIMIAVTGIIFKAYWVSFLVSAVGITAGVIHMKANKYSYILYII
                     QISLYAYVSWRNRFIGEAVLAAFYLLPLYIYSAIRWIRLGNKEKGDAQIFKITKKQII
                     ILAVACVAVTGGYGYVLSLLKSNLPYANALGTFLSVAAGYLSAKRVKEQWLFWISYSL
                     IFSGIWISTLGSDTSNVTLILQNIPYLYINISGLIKWNKLYKEAHAEV"
BASE COUNT          564 a          330 c          319 g          466 t
ORIGIN      
        1 gcaaactata tcatcgtaaa aagtatcccg agagtctcta cctgcttgct atgcttgatt
       61 atgtaagccg agtaaacaat atacctctgt gcacaaaata caaggcatta cgcaaagtaa
      121 aactggagga aacactttat cccgccggca tactggccat ggctgttata agcggacagt
      181 ctaagattaa ggaagatgca ttatctcaat ccattccgga attcttgcgt cataacatta
      241 tagaaagtga ggtacgcaat gtcatctgat acagttcatg cattcaataa agataatttg
      301 gatacttact taaaagcgtt agctaaactc ttccggaagc aaaacggtaa aagagctact
      361 gcagagatta ttcttgtggg cggagcagct gttttggtta actatggctt tcgggacatg
      421 actgcagata tagatgccat cattcacgct tcagcttcca taaaagatgc aatcaaccaa
      481 atcggtgatg agtataattt gcccaatggc tggttgaata ccgactttat gcataccgct
      541 tcctattccg agaaactcaa tgaattttcc gtgtattaca aaaccttcta cggcattctt
      601 caagtgcgta gcatatccgc agaatatctg attgcaatga aatgtaagtc cggacgaaag
      661 tataagaatg atatgtccga catcataggt atcttggcag aacataaaaa gcgagaagaa
      721 cctttaactt gggaaaaagt taatcaggca gtaattaacc tatatcaaga ctggaacggt
      781 attgcaaagg aaataaaaga ctttattcgg aatgccgtta tgactgataa ctatgaagaa
      841 gtactaaagc agataaaaga gcaggaacag ctcaccaaaa acatacttat tcaatttcaa
      901 aaagagcatc cgcatactgt aacggaatca aatgttgaca gtattttgag aatgcttgaa
      961 tccaaaaaga tagccgataa tattgaaggt aaaaatcgca tttgtacaga agatgaaatg
     1021 ttatagaatt atgaaaaaca atctgcacta tgttaaactt ccgcatgtgc ttctttgtac
     1081 agtttgttcc attttataag accggagata ttaatataaa ggtagggtat attctgcagt
     1141 atcaatgtaa cattggatgt atcggagccc aatgtactta tccatattcc ggaaaagatt
     1201 aagctgtacg atatccagaa aagccattgt tcttttactc tctttgcgga aagatacccc
     1261 gctgccaccg ataaaaaagt acccaatgcg tttgcatacg gaagattgga tttcagaaga
     1321 ctcaatacat agccgtaacc tcctgtcacc gcgacacatg caacggcaag tataataatc
     1381 tgtttcttgg ttatcttaaa tatctgggca tcgccttttt ccttattacc caaccttatc
     1441 catctgattg cggaatatat atacaaaggc aacagataga atgccgccag aaccgcttca
     1501 cctataaatc tgttgcgcca ggaaacatat gcatacagac tgatctggat gatatacagt
     1561 atataactgt atttgttggc tttcatatgt atgacgcctg cggttattcc cacggcggat
     1621 accagaaaag acacccagta tgctttaaag ataatacccg ttaccgcaat cattattat
//
LOCUS       DAOPWC010000356         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_80476_length_1678_cov_2.611830, whole genome shotgun sequence.
ACCESSION   DAOPWC010000356 DAOPWC010000000
VERSION     DAOPWC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80476_length_1678_cov_2.611830"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PKJ65_07055"
     CDS             <1..1455
                     /locus_tag="PKJ65_07055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ41628.1"
                     /translation="PYQHGEVFVTDDGAETDLDLGHYERFIDENLNKYSNLTSGKIFW
                     NVLNKERKGEYLGETVQIIPHITNEIKSFIYRVGKKTNANVVITEIGGTIGDIESTYI
                     LEAIRQISLELGPGNCLFIHVTLVPYIASSGEYKSKPTQHSVKELHSMGISPDMIVLR
                     CNDYLDDSLKKKISLFCNVKEDCVIENLDMDVLYEVPLELEKLDFSSLVCRELGLVTK
                     DPDLTEWTAMIERLKNIEEEVVIALVGKYIQLHDAYLSVVEALKHAGLENNVKPVIKW
                     VDSEEVTAENASEVFADCHGIIIPGGFGVRGIEGKVIACNYARVNNLPYFGICLGMQI
                     AVVEFARNVCGLADANSGEFDERSHHKVVDIMPDQYVSMVKGGTMRLGAYPCKIKDGT
                     LMHQAYKTDEVSERHRHRYEFNNEYRKQFEAKGMVVSGTSPDDILVEAIELPKNDFFL
                     GVQFHPEFKSRPNRPHPLFSLFVKHAVRKKSKKK"
BASE COUNT          538 a          300 c          410 g          430 t
ORIGIN      
        1 ccctatcagc acggtgaagt ttttgtgacc gatgacggag cggaaaccga tttggattta
       61 ggtcattacg agcgctttat cgatgaaaat ctcaacaaat actccaacct cacctcggga
      121 aagattttct ggaatgtact caataaagaa agaaaagggg aatacttagg agaaacggtt
      181 cagataatcc ctcatataac caacgaaata aaaagcttca tataccgcgt aggtaaaaag
      241 accaacgcca atgtggtaat aacggaaata ggcggaacca taggtgatat agaaagtaca
      301 tatattttgg aagcgataag gcagatatct ttggaattag gtccgggtaa ctgccttttt
      361 attcatgtaa cgcttgtacc gtatatagcc agttccggag aatacaaatc caaacccact
      421 cagcactcgg taaaagagtt gcattccatg ggaatatctc cggacatgat tgtgcttcgc
      481 tgtaacgatt atctggatga ttcattaaag aaaaagatat cgcttttctg taatgtaaaa
      541 gaagactgcg taatagaaaa tctggatatg gatgttttgt atgaagttcc tctggagctg
      601 gaaaaactgg acttctcttc tttggtatgc agggaattgg ggctggtaac aaaagatccg
      661 gatctgaccg aatggaccgc tatgattgaa aggctgaaga acattgaaga ggaagtggtc
      721 attgctctgg taggcaaata tatacaactc catgatgcct atctttcggt agtggaagct
      781 ctgaagcatg cgggattgga gaataacgtc aagcctgtta taaaatgggt ggacagcgaa
      841 gaagtcaccg ctgaaaatgc atcggaggtt tttgccgact gccacggtat tattattccc
      901 ggaggattcg gggttcgggg aattgaaggt aaagtcatcg catgcaacta tgccagagtc
      961 aataatcttc cgtatttcgg aatatgttta ggtatgcaga tagcggtagt ggaatttgca
     1021 cgtaacgtgt gcggattggc cgatgccaac tccggagagt ttgatgagag aagtcatcac
     1081 aaagtggtgg atatcatgcc cgatcagtat gtaagtatgg taaagggagg caccatgaga
     1141 ttgggtgcat atccctgtaa gattaaagac ggaactctta tgcaccaagc gtacaagacc
     1201 gatgaagttt ccgaacgtca cagacaccgc tatgagttca ataatgaata cagaaaacaa
     1261 ttcgaggcta agggaatggt agtttccgga acctcaccgg acgatatatt ggtggaagcc
     1321 atagagttgc cgaagaacga ctttttcctg ggcgtacagt tccatccgga attcaaaagc
     1381 cgtcccaaca gaccgcaccc gctgtttagt ctttttgtaa agcatgcggt gcgcaaaaaa
     1441 agcaaaaaga agtaaagtcg agtaaagacc ggtatttgcg agtaattaaa agcaatcggg
     1501 acatatttac ggataaacag catatttgac cgattttgcg gtttgagaat atgtgctatg
     1561 taaactacaa tatctttagc actctaatgt cttgagtgct aaaatatgga caatcggagg
     1621 tagaattatg gtaatcaaac cattaagtga cagaatagta ataaggatgt tggaatcg
//
LOCUS       DAOPWC010000357         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_81124_length_1669_cov_4.433705, whole genome shotgun sequence.
ACCESSION   DAOPWC010000357 DAOPWC010000000
VERSION     DAOPWC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81124_length_1669_cov_4.433705"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKJ65_07060"
     CDS             <1..1140
                     /locus_tag="PKJ65_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ41629.1"
                     /translation="VRQQRTAEGHAVRASYLYAGMADIAYRYKDKELLAACKAIFSNI
                     VKKRMYITGGTGSSHLGEAYTLDYDLPNTKGYAETCAAIALAFFAQRMLLSDADSVYA
                     DTVERIIYNGMLSGVSLDGKAFFYENPLEIQPVLNNRNVATTLTERNAVTQRKEVFGC
                     SCCPPNVIRFIGAINEYIYSYNKDTLFINQFISSDLEYENIRAEIRTEFPNCGKVYIK
                     VSGVNKVAVRKPYWCKYLKLVINDVRSKYSSKKGYIYINLPKGQNRIRLEMDITPRLV
                     YSNPKVYDNSHKTAVMRGPVVYCAEAVDNDFPLNNIVFGKELNYKTVKSDQLDAYMLD
                     VDIKIPQSIDDLYTFTPFKYRKKTLRMIPYYCFANRGETEMRVWL"
BASE COUNT          570 a          269 c          370 g          460 t
ORIGIN      
        1 gtaaggcaac agaggacggc ggaaggacat gcagtcagag catcttatct ctatgcggga
       61 atggcggata ttgcgtacag atacaaagac aaggaacttc ttgctgcatg taaagccatt
      121 ttttccaata tagtgaaaaa aagaatgtat attaccgggg gaaccggatc atcgcacctg
      181 ggtgaagcat atacattgga ttacgatctt cccaacacca aaggatatgc cgaaacatgt
      241 gcggcaatag cgttggcatt ctttgcgcaa cgcatgcttt tatctgatgc cgactccgta
      301 tatgcggaca cggtggaaag gattatttac aacggaatgc tctcgggagt atctctggac
      361 ggaaaagcgt tcttttatga aaatcctttg gagattcagc ccgttttgaa caacaggaat
      421 gtggccacga cacttaccga gagaaatgcc gttactcaga gaaaggaagt gttcggctgt
      481 tcctgctgtc ctcctaatgt aataagattt ataggagcaa ttaacgaata catatattca
      541 tataataagg ataccctgtt tataaaccag ttcatatctt ccgatctgga gtatgagaat
      601 ataagggcag aaatcaggac ggagtttccg aactgcggga aagtatatat taaagtttcc
      661 ggagtaaata aggttgccgt aagaaaacct tattggtgta agtatctgaa attggtaatt
      721 aatgatgtaa gaagcaaata ttcttcgaaa aaaggttaca tatacatcaa tctgccaaaa
      781 ggacagaaca gaatcagatt ggaaatggat attacaccga gactggtata cagtaacccg
      841 aaagtgtatg acaattccca taagaccgcc gtaatgagag gtcctgtggt atattgtgcg
      901 gaagctgtcg ataacgattt tcctcttaac aatatagttt tcgggaaaga gctcaattac
      961 aaaacagtaa agtccgatca gctggatgcc tatatgctgg atgtggatat aaaaataccc
     1021 caaagcattg acgaccttta tacctttacc ccgttcaaat accgaaagaa aacattgaga
     1081 atgattccct attactgctt tgccaacagg ggagaaaccg agatgagggt gtggttatga
     1141 gtagattcaa agaaaagcgg gaactgaaaa atagcactca cggtgctatt ttacggagcg
     1201 aaactttgta tgaggagttg ttaatattgt ttcggtatta ttagcatgtt ttttggtttc
     1261 ttttagagga ggaaggaatt attgaagtaa ctgctagtaa tacaacatga gtataaaaag
     1321 aaagtgttaa taaagtgtga acaaaaaatt aactttccat gaattctttt gctcttttgt
     1381 tgacttgcgc ctatgttgaa agtatactcg ggacatatta acttcaaaag aaagtgatat
     1441 cggacaagaa aagagatttt tatttacagt attaacagaa aaaccgttaa ttgtttatta
     1501 cgaaagagaa agaggcatta ataatgaaaa aaggaaaaaa gaaagtatgg atttctgtaa
     1561 ttggcatcat tgttatttta acagcaacag ctttattggt gtcttgtcaa agtaaagaag
     1621 attatggcaa ccgcatagtc attaagagaa tagacggaaa aggttctct
//
LOCUS       DAOPWC010000358         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_81286_length_1667_cov_3.225806, whole genome shotgun sequence.
ACCESSION   DAOPWC010000358 DAOPWC010000000
VERSION     DAOPWC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81286_length_1667_cov_3.225806"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKJ65_07065"
     CDS             complement(<1..723)
                     /locus_tag="PKJ65_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ41630.1"
                     /translation="MKLLNNKVAVITGAAQGLGEALAQRLDKEGCSIVLADLNYEKVC
                     EVAKPLKKAVACKVDVTKEAQVDAMTQLAVDTFGRIDIFVNNAGIVISSPTDEFDVNK
                     WRLVQDVNLWGYFICAKSAVKHMLKNKNGASFIQINSKSGKKGSFRNSAYAASKFGGI
                     GLTQSLALEYAKDNIRFNAICPGNLLNSPLWVDSLFKQYAKNQGITEEEVRQKYLDQV
                     PMGRSCEYEDVANVMVFLASDMS"
     gene            complement(819..953)
                     /locus_tag="PKJ65_07070"
     CDS             complement(819..953)
                     /locus_tag="PKJ65_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41631.1"
                     /translation="MSDKDDKDVGRSKQEFRRKIIKTVILALLIIALSALLIYWYFLR
                     "
     gene            complement(950..>1667)
                     /locus_tag="PKJ65_07075"
     CDS             complement(950..>1667)
                     /locus_tag="PKJ65_07075"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNZ41632.1"
                     /translation="INERVVFSRLRPVVKDQLLKEKIVDKKEIGRFVSKFRYPLYYLD
                     FETIQYAIPPYDGMKPYQQTPFQYSLHIEYEDGRLEHKEYLASDGFDCREQLATRLVN
                     DIPEGACSVAYNMAFEKGVIRDLAEYFPHLRDKLTDIYDNMLDLMIPFKNHWYYDKAM
                     QGSYSIKAVLPALFPDDYTLDYENLEGVKRGDQAMAAFRKMPSMSEEEKQQVRNQLLE
                     YCKLDTYAMVMIMRKLREQL"
BASE COUNT          452 a          394 c          285 g          536 t
ORIGIN      
        1 ggacatatcg gaagctaaga aaaccattac atttgcaaca tcctcatatt cacaggatct
       61 tcccataggt acctgatcaa ggtacttctg acggacttcc tcttcggtta ttccctggtt
      121 ttttgcatac tgcttaaaaa gactgtcaac ccataacggg gaatttaaaa ggttacccgg
      181 acatatggca ttgaatcgga tattgtcttt tgcatactcc agagcaaggc tctgagtaag
      241 tcctataccg ccgaacttcg atgcggcgta tgcactgttt ctaaaagagc cttttttgcc
      301 cgatttactg ttaatctgta taaaagatgc tccgttttta ttctttaaca tatgttttac
      361 agccgacttg gcacatatga aatatcccca aagattgaca tcctgcacca gtctccattt
      421 gtttacatca aactcatcgg tgggactgga tataacaatg ccggcattgt tgacaaatat
      481 atcaattctt ccgaatgtat ccacagccag ctgagtcatt gcatctactt gtgcctcttt
      541 ggtgacatcc actttacagg ctacggcttt tttcagcggc tttgccactt cacatacttt
      601 ttcgtaattc aaatccgcca aaacgattga acacccttct ttatccagtc tttgagccaa
      661 tgcctcgccc aaaccctgtg cggctcccgt tatgacagca actttattat tcaataattt
      721 cataaaaaag ctcctaattg ttattcttac ctttattata tcccaacggt tttcaaaaac
      781 atataggatt gtgtcttttt aactaaagaa aaccgctgtt atctcaaaaa ataccagtat
      841 ataaggaggg cggacagtgc aattataagt aaggccaata taacggtctt tataattttt
      901 cttctgaatt cctgcttact ccgcccgaca tctttgtcat ctttatcact cataattgct
      961 cccttagttt tctcattatc atcaccatgg cataggtgtc caacttacag tattccagta
     1021 actgattccg aacctgctgt ttttcctctt ccgacatgga aggcattttt ctgaacgcag
     1081 ccatggcctg atctcctctt tttactcctt caagattttc ataatccaaa gtataatcat
     1141 ctggaaaaag agcgggcaat accgctttga tggaatagct tccctgcatt gccttatcat
     1201 aataccagtg gtttttaaag ggtatcataa gatccagcat attgtcatat atatccgtaa
     1261 gcttatcccg aaggtgagga aaatattcgg caaggtctct gatgacacct ttttcaaacg
     1321 ccatgttata tgcaaccgag caggcgccct caggtatatc gtttaccaga cgtgtggcca
     1381 attgttctct gcagtcaaat ccgtccgatg caagatactc cttatgctcc agcctgccgt
     1441 cttcatactc tatatgcaaa gagtattgaa aaggtgtctg ctgatacggc ttcatgccgt
     1501 catacggcgg tatggcatac tgtatcgttt caaagtctaa ataatacaga gggtaacgga
     1561 acttgcttac aaatcgaccg atctcttttt tatctactat cttttctttc agtagctggt
     1621 cttttacaac cggtctcagc cgtgaaaata caactctttc attaata
//
LOCUS       DAOPWC010000359         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_82564_length_1650_cov_3.700313, whole genome shotgun sequence.
ACCESSION   DAOPWC010000359 DAOPWC010000000
VERSION     DAOPWC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82564_length_1650_cov_3.700313"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKJ65_07080"
     CDS             complement(<1..676)
                     /locus_tag="PKJ65_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNZ41633.1"
                     /translation="MNEDKRFAVLIDAENVSEKYIKYIFDELAKSGTITYKRIYGDWT
                     RPELSSWKKILLDYSITPIQQYGYTTGKNATDSAMIIDAMDILYDDNVEGFCIVSSDS
                     DFTRLATRLREAGKYVVGMGEKKTPAPFIASCNQFKYLEILGQEENNNKQKDMEDSIT
                     PLEKVIKAIETIADDISDDDGWSPLSDVGNILSKMFPDFDVRNYGFSKLTPFVSSLKK
                     FDIKSVN"
     gene            complement(1292..>1650)
                     /locus_tag="PKJ65_07085"
     CDS             complement(1292..>1650)
                     /locus_tag="PKJ65_07085"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518947.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ41634.1"
                     /translation="EPHNTVMQWAAPNPDFLCTTDVLEAAKDADVLYTDVWASMGHEK
                     QVQIRTEAFKGYQINRKVTASAKPDAIVLHCLPAHREQEITEEVFEQHAKEIFEQAEN
                     RIHAQKAIMVLLMKDK"
BASE COUNT          478 a          314 c          302 g          556 t
ORIGIN      
        1 ggtttacgga ctttatgtcg aattttttca gcgacgacac aaacggcgtg agtttgctga
       61 atccgtagtt tcgtacatca aaatcgggaa acattttgga cagtatattg cccacatcgc
      121 ttaacgggct ccaaccgtca tcatcggata tatcatcggc tattgtttca atagctttta
      181 ttaccttctc cagaggagta atggaatcct ccatgtcttt ttgcttattg ttgttctctt
      241 cctgacccag tatctccaag tatttgaatt ggttgcagga tgctatgaag ggggcggggg
      301 ttttcttttc tcccatacct acaacatatt tacccgcttc acgaagcctg gttgccagtc
      361 ttgtaaagtc cgaatcggaa gaaactatac agaatccttc aacattatcg tcatacagta
      421 tatccatggc atctataatc attgccgaat cggtggcatt tttacctgtt gtatatccgt
      481 actgctgtat aggtgtgatg gaatagtcta aaagtatttt cttccaggaa ctcagttccg
      541 gtctggtcca gtctccgtat atccttttat aggttatcgt tcccgatttt gccagttcat
      601 caaatatata ttttatatat ttttccgaaa cattctcggc atctataagt actgcaaatc
      661 gtttatcttc attcatattt ttttacctca ttatatataa tatcatatat taacaatgtt
      721 ttcttctaac atcatatcat gtaataaagg ctaatatata cataaaggca aatagttcaa
      781 tgaatgttga actatttgtg tcatataaca tcctgtatgt ctaaattatg caaggatata
      841 taatgatact tgattttgca agaaaaattc atataataag tggcgcaata aaaataatat
      901 atttggaggt attgaaatga attttcaaaa aatttgggaa aacattaaaa cccatgaagg
      961 tgaagtgttc aaaactaaaa ggaatctacc ttttacatat acggttgaag gtaattatgt
     1021 gattccgagc aggacagatt accctttgca taaaacgcag tttgttaaag cattggaagt
     1081 cgataatctc acaggacctg gaaaaattaa taaaatagtc agaggtccgg catatgttta
     1141 tggcattttg aaggacccgc gtataagagg gtagaagtaa actcacatat gataaatatg
     1201 acagagtact aaagataaca ttaataaaca tcaagacgat gactttatca ccgaccgttt
     1261 taaggaatca aatatgcagt ctttcttcta cttacttatc tttcatcagc agtaccatta
     1321 ttgccttttg tgcatgaata cggttttccg cctgctcgaa tatttccttt gcatgttgtt
     1381 caaatacctc ttcggttatt tcctgctccc tgtgagcggg aaggcagtgt aatactatgg
     1441 catcgggttt ggcagaagct gttactttac ggtttatctg atagcctttg aatgcttccg
     1501 ttcttatctg tacttgcttt tcatgaccca tggatgccca gacatcggta tacaatacat
     1561 cggcatcttt tgcggcttct aaaacatcgg ttgtacataa aaagtcggga ttcggtgctg
     1621 cccattgcat cactgtattg tgcggctcat
//
LOCUS       DAOPWC010000360         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_82580_length_1650_cov_3.105329, whole genome shotgun sequence.
ACCESSION   DAOPWC010000360 DAOPWC010000000
VERSION     DAOPWC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82580_length_1650_cov_3.105329"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..368
                     /gene="secG"
                     /locus_tag="PKJ65_07090"
     CDS             123..368
                     /gene="secG"
                     /locus_tag="PKJ65_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNZ41635.1"
                     /translation="MSEALKIILGIVHIIVCLATIIVVLMQSGKSEGLSGAITGGAET
                     FFGKGKGRAIDKLLSKYTVYIAIFFAVTSIFLYLVLK"
     gene            548..1621
                     /locus_tag="PKJ65_07095"
     CDS             548..1621
                     /locus_tag="PKJ65_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ41636.1"
                     /translation="MLIRLIGLLVAFGLFIFLICQKKVLKWKKAISVIVSFVLVVFMI
                     IQVFFPFIPAKKPTGPYGVITETAFYKYNTDIPGMATNGNEREIPVKVWFPKDAQAKS
                     HPLFLFSPGSFGVAESNETLFLALASRGYVVVGLNHPYHSFSCKMSDGRNINMDGKFI
                     QSVMTSQGSEDLPGTLSSFKEWLKVRIDDINTVLDCISDFQNDYEQYIDKERVVLSGH
                     SLGGSAVLAVGRERAQEIKAMAVLEAPFAADIVGIDKDRYIFTDQPYPIPVLHVYSDA
                     LYGKMDDITTYDMNTRLMKSDNPMYVNKHIKGVGHLGLTDMRLVTPVITYLMDGGLDT
                     RPAAETLSELNEYVLDFLNIYNN"
BASE COUNT          493 a          258 c          368 g          531 t
ORIGIN      
        1 catgcccctg tagaacggaa cttagttatg caatctaatg aatgaaaaca gcaagaaaaa
       61 atatacttgc tatacaaaat cgtttatgtt ataatctcgt agttaagttt tggaggcaaa
      121 aaatgagtga agctttaaag ataatattag gtatagtgca tataattgtg tgtctggcaa
      181 caatcatagt ggtattgatg cagtcgggca aaagcgaagg actttcggga gctattacag
      241 gcggagcgga aacctttttc ggtaagggta agggcagagc aattgacaag cttctttcca
      301 agtataccgt ttatatagca atattctttg cggttacatc catcttctta tatctcgtac
      361 tcaaataaag tgcggatacg gaatatttta aattcttaaa aaggcttata agccttttct
      421 ttttactttt ttgttgtgat ttaatgggaa atttactgaa tatgcaatag ttttacaata
      481 gtcgccgttt aagaaattga acagtattat ccgttggaat ataatttaac gggaggttat
      541 aaagactatg cttatacgat tgataggatt attggttgct ttcggtttat ttatcttcct
      601 tatctgtcag aagaaggtgc tgaagtggaa aaaagcgata tcggtaattg tctcatttgt
      661 tttggtagta tttatgataa ttcaggtatt ttttccgttt ataccggcca agaaacctac
      721 cggaccttac ggtgtcataa ccgagacggc attttataag tataataccg atattccggg
      781 aatggctact aacggcaatg aaagggagat tcctgtcaag gtgtggtttc cgaaggatgc
      841 acaagcaaaa agtcacccct tgtttttatt ctctcccgga tctttcggtg ttgccgaatc
      901 caatgaaact ttatttttag ccctggcatc acgaggctat gttgtagtag gcctgaatca
      961 tccgtaccac tctttcagct gtaagatgtc tgacggcagg aatataaata tggacggaaa
     1021 gtttatacaa agtgttatga cttcccaggg ttcggaagat ttgcccggta ctttaagttc
     1081 atttaaggag tggttaaaag tgcggattga tgatatcaat acggtacttg attgtatttc
     1141 ggactttcag aatgattatg aacagtatat tgataaagaa cgggtggttt tatccggaca
     1201 ttctttgggc ggttccgctg tgttagccgt aggtcgtgaa agagcgcaag agattaaagc
     1261 aatggcggtt ttggaggctc cgtttgcagc ggatattgtg ggaattgaca aagaccggta
     1321 tatatttacc gatcagccgt atccgatacc cgttttgcat gtttattcgg atgcattgta
     1381 tggaaaaatg gatgatatta ctacatatga tatgaataca cgacttatga aaagcgacaa
     1441 tcctatgtat gtgaataagc atattaaagg tgtcggacat ctgggattaa ccgatatgag
     1501 actggtaaca cccgttatta catatttgat ggacggagga ttggatacgc gtcctgcggc
     1561 ggaaaccttg tcggagttaa atgaatatgt actggatttt ttaaatatat acaataatta
     1621 aacgaatttt atatttccga ttaatttaca
//
LOCUS       DAOPWC010000361         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_82657_length_1649_cov_3.765997, whole genome shotgun sequence.
ACCESSION   DAOPWC010000361 DAOPWC010000000
VERSION     DAOPWC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82657_length_1649_cov_3.765997"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1029)
                     /gene="arcC"
                     /locus_tag="PKJ65_07100"
     CDS             complement(91..1029)
                     /gene="arcC"
                     /locus_tag="PKJ65_07100"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNZ41637.1"
                     /translation="MIKRIVVALGGNAILTKDPTAEGQIQALRETSEKLVELIKHDYE
                     LIITHGNGPQVGNLLLQQEAAHSKSNPAMPVDTAVAMTQGSIGYWLQNTLDEAICKSG
                     MEKSVATILTQVVVAEDDPAFRNPTKPIGPFFSKEQAEKRAEESGDTYVEDAGRGYRK
                     VVPSPKPVSVIEADTIRTLVENGVITISAGGGGIPVVKRGTKYVGVEAVIDKDFAAAK
                     LAEIVKADYLVILTGVENAYINFNKPDQKKLEHVTVTEMKQYIAEKQFAAGSMLPKIQ
                     AAISFVESVEDGKAFITSLDNVNAITGNGSATVITA"
     gene            complement(1187..>1649)
                     /locus_tag="PKJ65_07105"
     CDS             complement(1187..>1649)
                     /locus_tag="PKJ65_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41638.1"
                     /translation="TTDGMSIPVITEIAEYFEEEIGYEIPPRTPFVGRNFNVTRAGIH
                     ADGMLKDVEIYSIFDTEALLNRPASVAVDSHSGLAGVAHWINSYFRIKDKEMAVKKTD
                     PVVARVKELVDKEYAEGRNTVMGDGELESILKFVDYDEYEKLSRKYARKRK"
BASE COUNT          423 a          410 c          295 g          521 t
ORIGIN      
        1 ttcaacaaca ttatagcagc taactgggct tttggcaaga attcatttcg ccttttggcg
       61 cattttcact tggtgttttg gcataaaact ttatgcagta atgactgtag cagagccgtt
      121 acctgtaatg gcattcacgt tatccaagga agtaatgaat gctttaccgt cttctacgct
      181 ttctacaaag gaaatagcag cttggatttt cggcaacata ctgcctgcgg caaattgctt
      241 ctccgcaata tactgtttca tttcggtaac ggtaacatgt tccagttttt tctgatcagg
      301 tttattaaag tttatgtaag cattttccac accggttaat attaccagat aatcagcttt
      361 tacaatctct gcaagttttg cagctgcaaa gtctttatca ataaccgctt ccacgcctac
      421 atattttgta ccgcgtttga caacgggaat accgccgccc cctgcggaaa ttgtgatgac
      481 tccattctct acaagtgttc tgattgtatc ggcttcaata acggaaaccg gcttgggcga
      541 aggaacaacc ttacgataac cccgacctgc atcttccaca taagtatcac cagattcttc
      601 agctcttttt tccgcttgct cttttgagaa aaacggtccg atcggttttg tgggatttct
      661 gaaagccgga tcatcctctg ctacaactac ctgcgttaat atggtggcaa ccgacttctc
      721 cataccgctt ttacatattg cttcatccaa tgtgttctgc aaccaatacc ctatacttcc
      781 ctgtgtcatg gcgacagcgg tgtctaccgg catggcagga ttggattttg agtgtgcagc
      841 ctcttgctgt aaaagtaaat tccccacttg cggaccgttg ccgtgagtga taataagctc
      901 ataatcgtgt tttatcaatt cgaccagttt ctccgatgtt tcacgaagtg cctgaatctg
      961 cccttccgct gtaggatcct tagttaaaat cgcattaccg cccaatgcga caacaattct
     1021 cttaatcata aatacctctc tttgacataa tacagcaggt attaccaccc gttattatgt
     1081 cactgtaagt aggataataa aaaacgggat gaatgtccac aactaataca attttttttc
     1141 aaaataacat tgccgcagca tataggatta tacggcagct acaccgttat tttctcttcc
     1201 ttgcatactt gcgacttaac ttctcatact catcatagtc aacaaacttt aatatactct
     1261 ccaactcgcc gtcacccata acggtattcc tgccttctgc atattcctta tccacaagtt
     1321 cctttaccct ggcaacaacg ggatcggtct tttttacagc catttcttta tcttttatcc
     1381 tgaaatacga gttaatccaa tgagcgactc ccgccaatcc gctgtgagaa tccacggcta
     1441 ccgaagccgg tctgttaagc agtgcttcgg tatcgaatat gctgtatatc tccacatcct
     1501 taagcatacc gtccgcatgt attcccgctc tggtaacatt gaaatttctg cctacaaaag
     1561 gagttctcgg cggtatttca taacctattt cttcttcaaa gtattcggca atctcggtaa
     1621 taacgggaat gctcattccg tcggtcgtt
//
LOCUS       DAOPWC010000362         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_82809_length_1647_cov_3.260050, whole genome shotgun sequence.
ACCESSION   DAOPWC010000362 DAOPWC010000000
VERSION     DAOPWC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82809_length_1647_cov_3.260050"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PKJ65_07110"
     CDS             complement(<1..1262)
                     /locus_tag="PKJ65_07110"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ41639.1"
                     /translation="MLVICLAIVLLLITANNIILAEISERDKKRLLEETDRRITLMYT
                     KQVEMFDSLASNIANSDMLSEYADNSYYLDDIMHDNADIFRYLKLMSDTNEYIDHIMI
                     YDYELEYLLNGRNEFTAYVDKAGFPWFIQRVEGRIPSADLDKYCMWHIYDEYIAITYK
                     ADTLNPSLNRRVFITLNITKTDFARFLDGPTLTENSTNGFISKDDNFFFSGSEEILMY
                     KTRGQAFDYFNDLLIKEKVTMEHNYILYYLQYSPLDCDYNLGVVYLIPDKDISSELYI
                     YTVVISIGVSLFILILCYSIFRRILETNIQRPISRLIRGMEKVGGGDFNSKLDDSICK
                     DNEIKGLFHAYNSMTDRATVLIEELYQAELYRKQLELQTLQDKIDPHFLYNTLDTINW
                     TAKEHNVEEISKIVVALSTMYRKIFNKG"
     gene            complement(1329..>1647)
                     /locus_tag="PKJ65_07115"
     CDS             complement(1329..>1647)
                     /locus_tag="PKJ65_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41640.1"
                     /translation="RIGDNFDGLFFHVHSVGHHVIPAVCEVDKIKFVEISNDPNAPRG
                     IEIYKKYEKELSNKIVTVALTQEEITDNLNFLRDKKVIVWYDASDVDDAKKAVDLVRN
                     KLG"
BASE COUNT          474 a          340 c          276 g          557 t
ORIGIN      
        1 ttacctttat taaatatttt tctgtacatg gtggaaagag ccacaactat tttagatatt
       61 tcttcaacat tatgctcttt tgccgtccag ttgatggtat ccagagtatt gtacagaaaa
      121 tgcggatcta tcttgtcctg caaagtctgc agttccagct gttttctgta gagctctgcc
      181 tgatataact cttcaatcag cactgtagct ctatcggtca tggagttgta ggcatgaaac
      241 agacctttta tttcattatc cttacatata ctgtcatcca acttggagtt aaaatctccg
      301 cctcctactt tttccatacc tctgattagc ctggaaatgg gtctttgaat attggtctcc
      361 aatattcttc ggaaaataga atagcacaat ataagaataa acaaagaaac ccctatcgag
      421 ataacaacgg tgtatatata taactccgaa gatatatctt tatccgggat aaggtataca
      481 actcccaagt tatagtcaca atccaaaggc gaatattgca gataatacag tatataatta
      541 tgttccattg tcactttttc ttttattaaa aggtcattga aataatcaaa agcctgtcct
      601 cgtgtcttat acatcagtat ctcttcgctg ccgctgaaga aaaagttgtc gtctttggaa
      661 ataaacccgt ttgttgagtt ttctgttaaa gtaggcccgt caagaaacct tgcgaaatcc
      721 gtttttgtga tattcaatgt tatgaaaaca cgacgattaa gtgacggatt gagagtatcg
      781 gccttgtatg ttatggctat atattcatca taaatatgcc acatgcaata cttatcaaga
      841 tcagccgaag ggattcttcc ttccacacgt tgaataaacc aaggaaatcc ggctttgtca
      901 acatatgcgg taaactcatt tctgccattc agcagatact caagttcgta atcgtaaatc
      961 atgatatggt caatatactc gtttgtatcg ctcataagtt tgagatagcg gaaaatatcg
     1021 gcattgtcat gcattatatc atcgagataa taactgttat ccgcatattc cgataacata
     1081 tcgctgtttg cgatattgct tgccagggaa tcaaacatct ccacttgctt tgtatacatc
     1141 aaagttatcc tgcggtcggt ttcttccaac agcctttttt tatcgcgttc ggaaatttca
     1201 gccaggatta tattattggc cgttatcaat aacaggacta tggcaaggca tatgaccaac
     1261 acaccaagca atttgttttt taagcttttc tgggcagtat cttttctcag tttttatctg
     1321 ttcctttctt atcccaactt atttctgaca agatcgacag cctttttggc atcatcaaca
     1381 tcgcttgcat cataccagac gatgactttc ttgtctctta aaaagttcag attatcggtt
     1441 atttcttctt gggttaaagc tacagttaca atcttattac tgagttcctt ctcatacttt
     1501 ttataaatct caatacctct cggtgcattg ggatcattag atatttccac aaactttatc
     1561 ttatccactt cgcaaaccgc aggtatgaca tggtgtccga ccgaatgtac atggaagaat
     1621 aatccgtcaa agttatcacc tattcgt
//
LOCUS       DAOPWC010000363         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_83360_length_1640_cov_2.936278, whole genome shotgun sequence.
ACCESSION   DAOPWC010000363 DAOPWC010000000
VERSION     DAOPWC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83360_length_1640_cov_2.936278"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKJ65_07120"
     CDS             complement(<1..529)
                     /locus_tag="PKJ65_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ41641.1"
                     /translation="MNGSDSLTERFILEMEKKIVNGEYDINTMLPSFRKLCDEYNVSR
                     SVINTAVAVLNTKGFVDIIPRKSVRVRNWKKQGTLLTMNSSIALGKLEPETVDSFFDI
                     FAFIQCNGVYEAASKATEEELIQLGKIIEQEALLKDEKKLAALYHEFYKKIADISGNM
                     AYSQVFMSLEQAIYAI"
     gene            complement(526..>1640)
                     /gene="gcvPB"
                     /locus_tag="PKJ65_07125"
     CDS             complement(526..>1640)
                     /gene="gcvPB"
                     /locus_tag="PKJ65_07125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ41642.1"
                     /translation="GGYLCEITGMKGATLQPAAGAHGEMTGIMIIKAYHQNRNDNKRT
                     KMIVPDSAHGTNPASAATAGYEIVQVKSDDLGLVDIDALAALMDDETAGLMLTNPNTL
                     GLFESNITKIAKIVHDKGGLLYYDGANLNAVMGIARPFDMGFDVVHFNLHKTMSTPHG
                     GGGPGSGPVCVREDLVRFLPAPVVCKDGDRYYLDYDRKDAIGRVKAFYGNYLVYIKAL
                     AYILTMGPDGLKKASETAVLNANYIMAGIKDNFVIPYDRFCMHEFVLSGSRQKEQYGV
                     STLDMAKRLMDYGYHPPTIYFPLIVHEGMMIEPTETESKETLDKFIEALNKIAKEAKT
                     NPELLHEAPHNTYVRRLDEVTAARNPVLKWEKEQDE"
BASE COUNT          408 a          410 c          291 g          531 t
ORIGIN      
        1 aaattgcgta tatcgcctgt tccaatgaca tgaaaacttg tgaatatgcc atattacccg
       61 atatatctgc tatcttttta taaaactcat gatacaaagc ggcaagcttt ttctcatctt
      121 taagtaatgc ttcctgctca attatttttc ccagctgaat cagctcttcc tctgttgcct
      181 ttgatgccgc ttcatatact ccgttacatt gtataaatgc aaagatatca aaaaaactgt
      241 ctacggtttc cggttccaat ttgcctaatg ctatagagga attcattgtc agaagtgtac
      301 cttgtttttt ccagttgcgt accctgacac tttttctcgg tattatgtcc acaaatcctt
      361 tagtattaag caccgccaca gcggtgttta ttacacttct tgacacatta tactcatcgc
      421 aaagtttacg aaaagagggg agcatagtat taatatcata ctccccgttc acaatcttct
      481 tttccatctc caggataaac ctttcggtta aactatcgct cccgttcatt catcctgttc
      541 cttctcccat ttaagtacgg gatttctcgc ggccgttacc tcatccaacc ttctgacata
      601 tgtgttatgg ggagcttcat gcagaagctc gggatttgtt tttgcttctt ttgctatctt
      661 attcaatgct tcaataaact tatccagagt ttccttactc tcggtttcgg taggctctat
      721 catcatgcct tcatgaacaa tcagagggaa atatattgtc ggcggatgat agccgtagtc
      781 cataagtctt tttgccatat ccaatgtgga tacgccgtat tgctctttct gtctgcttcc
      841 ggaaagcaca aattcatgca tgcagaatct gtcatacgga atgacaaaat tatcttttat
      901 tcccgccatt atatagttgg cattgagtac cgcggtctcg cttgccttct ttaacccgtc
      961 ggggcccatt gtcagtatat aggcgagtgc ctttatgtac acaaggtaat taccgtaaaa
     1021 tgcttttaca cgaccgattg catcttttct gtcatagtct aaataatatc ggtcgccgtc
     1081 tttgcagacc accggtgccg gaagaaatct taccaaatcc tctcgcacgc aaaccggtcc
     1141 gcttcccggg cctcctccgc cgtgaggtgt ggacattgtt ttatgcaaat taaaatgaac
     1201 aacatcaaat cccatatcaa acggacgggc aatacccata accgcattaa gattggctcc
     1261 gtcgtagtat agtaaaccgc ccttgtcatg cacaatcttt gctatctttg taatattgct
     1321 ttcaaataat cccaaagtat tgggattggt cagcatcagt cccgcggttt catcatccat
     1381 taaggcggcc aatgcatcaa tgtccaccag tcccaaatca tccgatttaa cctgtactat
     1441 ctcatatccc gccgttgccg cacttgcagg attggttccg tgagcggaat cgggaactat
     1501 cattttggtt cttttattgt cattcctgtt ctgatgataa gcctttatta tcattatacc
     1561 ggtcatttca ccgtgagctc ccgcagcagg ctgcaacgtg gcacctttca tgcctgttat
     1621 ctcacataaa tacccgccca
//
LOCUS       DAOPWC010000364         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_83673_length_1636_cov_2.871600, whole genome shotgun sequence.
ACCESSION   DAOPWC010000364 DAOPWC010000000
VERSION     DAOPWC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83673_length_1636_cov_2.871600"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..821
                     /gene="dapB"
                     /locus_tag="PKJ65_07130"
     CDS             69..821
                     /gene="dapB"
                     /locus_tag="PKJ65_07130"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNZ41643.1"
                     /translation="MNILLVGCYGKLASAIIKACSGKEDMNIACGVDLSVKDNPLFPV
                     FTAANDIPSSIDIDAVIDCSHPSGVVSTVRFCKKYKKPLVYATTGLDKEQMNLLHELS
                     KHVAVFQSANMSLGVNLLIKLCKQAATFLGNDFDIEIIEKHHNQKIDAPSGTALKVAD
                     EINQSLNNRMTYVYDRQSKREKRDKKEIGIHAVRGGNIVGQHEVIFAGQDETITISHG
                     AFSKDIFAQGALRAALFIKDQPAGMYGMNDLL"
     gene            complement(828..>1636)
                     /locus_tag="PKJ65_07135"
     CDS             complement(828..>1636)
                     /locus_tag="PKJ65_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41644.1"
                     /translation="PTETKFMKFMEILKTRFWNIAKYNMICVGIYALFIVFYAFFVSQ
                     IYFPVDVSDYQQGYSNFGELWAILILRIPYTLVFFTIPVVAFGPAVSGLAVVFRSYVR
                     RDDSFSSDFGDGMKKFFWKSLAVTAISTVITVCLGIAARFYQLNLQAGFWKTALLLII
                     AGFSLLFIMMHMYIYQILVEYNLSIWKLYRYSYVFAILRFFPNLLIIAGIAALTILPF
                     KIHILFGAAVMATVLFGLTSYIINFFTWPALEKHLDPIMKEQRINKVKLY"
BASE COUNT          574 a          320 c          323 g          419 t
ORIGIN      
        1 ctggtggaaa tggaagagaa aaacgcaaat cttctcaaag aagaactgat taatctggga
       61 gttataaaat gaacatatta ctagtaggct gttacggaaa gttggcaagt gcaataataa
      121 aagcatgttc cgggaaagag gacatgaata tagcatgcgg agtggatctt tcggtaaaag
      181 ataatcccct cttccccgtt tttaccgctg caaacgatat accgtcatca atagacattg
      241 atgcggttat tgactgttct catccttccg gagttgtatc taccgtccga ttctgtaaga
      301 aatacaaaaa gcctcttgta tatgcaacaa ccggtctgga caaagagcaa atgaacttac
      361 ttcatgaatt atcaaagcat gttgccgttt tccagtcggc caatatgagc ctgggggtaa
      421 atctccttat aaaactctgc aaacaagccg caaccttctt aggcaatgac tttgatatag
      481 agattataga gaaacaccac aatcagaaaa tagatgctcc gagcggtaca gctcttaaag
      541 tagccgatga aataaaccaa tcgctgaata accggatgac atatgtttat gaccgtcagt
      601 caaaaagaga aaaaagagat aaaaaagaaa taggcataca tgcagtaaga ggcggcaaca
      661 ttgtgggaca gcacgaagta atctttgccg ggcaggatga aacaataaca ataagtcacg
      721 gggcattttc caaagatata tttgcacaag gtgcattaag agccgcttta ttcataaaag
      781 accaacctgc cggtatgtac ggtatgaatg atttgcttta gacactatta atacaattta
      841 actttattaa ttctctgctc tttcattatc ggatctaagt gtttttccaa tgcaggccat
      901 gtaaagaaat taataatata cgaagtcagt ccgaaaagaa cggtagccat aaccgccgct
      961 ccgaaaagta tatgtatctt aaaaggaagt atcgttaaag ccgcaatacc cgctataatc
     1021 aaaagattgg gaaagaatct gaggattgca aaaacataag agtatctgta aagcttccag
     1081 atggatagat tatactccac aagaatctga tatatgtaca tatgcatcat gatgaataac
     1141 agcgaaaaac ccgctataat aagcaacagg gctgttttcc agaaacctgc ctgtaaattc
     1201 aactgataaa atctggctgc aatacccaga caaacggtta taaccgtgga aattgccgta
     1261 accgccaggg atttccagaa gaactttttc attccgtcac cgaagtcgga agaaaagctg
     1321 tcatctcttc ttacatagct cctgaaaact accgcaagcc cggagaccgc tggaccgaat
     1381 gccacaacag gtattgtaaa gaataccaaa gtgtaaggga ttcttaaaat cagtattgcc
     1441 cataattcac cgaaattgga gtatccctgc tgataatcgg aaacatcaac gggaaagtaa
     1501 atctgtgata caaagaatgc gtagaagact ataaagagtg cgtatatacc cacacatatc
     1561 atattgtatt tggctatatt ccagaacctt gtctttaaga tttccataaa cttcataaac
     1621 ttggtttccg taggtt
//
LOCUS       DAOPWC010000365         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_84405_length_1627_cov_3.033079, whole genome shotgun sequence.
ACCESSION   DAOPWC010000365 DAOPWC010000000
VERSION     DAOPWC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84405_length_1627_cov_3.033079"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKJ65_07140"
     CDS             complement(<1..630)
                     /locus_tag="PKJ65_07140"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNZ41645.1"
                     /translation="MIFTKDKITVKLYDSRKEMGEIAASDTAAKIEELLLVKDEINII
                     FAAAPSQNEFLASLVKENRIDWSRINAFHMDEYIGLEEDAPQRFGHFLKESIFGKVPF
                     KQVNYINGKALPSEECARYEALLKQYPADIVCLGIGENGHIAFNDPHVADFKDPQAVK
                     VVDLDQACRQQQVNDGCFQSINEVPTHAYTLTIPALLSADYMYCIVPGES"
     gene            complement(646..>1627)
                     /gene="nagA"
                     /locus_tag="PKJ65_07145"
     CDS             complement(646..>1627)
                     /gene="nagA"
                     /locus_tag="PKJ65_07145"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNZ41646.1"
                     /translation="FMDGTVEAYLGAAKMHAKHGTTSLLPTTLTSTNEELLKTFSIYK
                     EAKAKNTEGASFLGLHLEGPYFAYGQKGAQDPKYLRNPEPEEYNAILEASDDIMRWSL
                     APELKGAIEFGKVLRSKNILPSIAHTDAICEEVEEAFDAGFTHVTHLYSCMSTITRRN
                     AYRYAGALEATYLIDDMTVEIIADGVHLPKSLLRYVYKFKGPDKIALCTDSMRGAGMP
                     DGESILGSLQGGQKVIIEDGVAKLPDRTAFAGSVATADRLIRTMIGLADVPLEEAIRM
                     ITLTPARILKIDKTKGSIEIGKEADLVLFDKDININTTIINGKVVYKKTH"
BASE COUNT          397 a          359 c          340 g          531 t
ORIGIN      
        1 gctttctcca ggaacaatac aatacatata gtcggccgaa agcagtgccg gtatggttaa
       61 agtataggcg tgagttggta cttcattgat ggattgaaag catccgtcgt tgacttgttg
      121 ctgcctgcag gcctgatcca gatcaacaac tttaacagcc tgagggtctt tgaaatcagc
      181 cacgtgcgga tcattgaatg caatatggcc attttctcct attcctaagc aaacaatgtc
      241 tgcaggatat tgttttaaaa gcgcttcgta tcgggcacat tcttcggagg gaagagcttt
      301 cccgttgata taattaacct gtttgaaggg aactttcccg aaaatgctct cttttaagaa
      361 atgtccgaat ctttgaggag catcctcttc caaccctatg tattcgtcca tgtgaaaggc
      421 atttattctg ctccagtcga tccggttttc tttgactaat gaagccaaaa attcattttg
      481 agaaggggct gctgcgaata ttatgtttat ttcatctttt acgagtagca actcttcaat
      541 tttagctgct gtgtcgcttg ctgctatttc tcccatctct ttacgggaat cataaagctt
      601 tactgttatt ttatcttttg tgaaaatcat ttttttctac gtattttagt gagttttttt
      661 atacacaacc ttaccattta ttatggtagt attgatattg atatctttat cgaaaagaac
      721 aaggtcggct tcttttccta tttctattga accttttgtt ttatctatct ttaatatacg
      781 agccggagta agtgtaatca ttctgattgc ctcttccagg ggtacatccg ccaacccgat
      841 catggtacgg ataagtcggt cggctgtagc aacgcttccc gcaaaggctg tgcggtcggg
      901 tagtttggca acgccatcct caattattac tttttgtcct ccttgcagac tgcccaggat
      961 tgactctcca tccggcatac ctgccccccg cattgagtca gtacataaag ctattttatc
     1021 aggccccttg aacttatata cataccgcaa cagcgatttg ggcagatgga ctccatccgc
     1081 aataatctcg actgtcatgt catctatcag atacgtagct tccaacgctc cggcatatcg
     1141 gtatgcgttg cggcgtgtga tggtagacat acaggaatac agatgcgtta catgagtaaa
     1201 acctgcgtca aaagcttctt ccacctcttc gcatatggca tctgtatggg caatggatgg
     1261 caagatattt ttagatcgaa gtactttgcc aaactcaatc gcccccttta attcgggggc
     1321 cagactccaa cgcattatat catcggaggc ttccaggatg gcattgtatt cttcgggttc
     1381 gggattccga aggtacttgg ggtcttgtgc tcctttttgc ccgtatgcaa aatagggacc
     1441 ttccagatgt aaacctaaaa aggaggcgcc ttccgtattt tttgcctttg cttctttgta
     1501 tatactgaat gttttgagta attcttcatt tgtacttgta agcgttgttg gcaataggga
     1561 agtagttcca tgttttgcat gcattttggc agcgcccaga taggcttcaa cagttccatc
     1621 cataaag
//
LOCUS       DAOPWC010000366         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_85464_length_1614_cov_3.064785, whole genome shotgun sequence.
ACCESSION   DAOPWC010000366 DAOPWC010000000
VERSION     DAOPWC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85464_length_1614_cov_3.064785"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKJ65_07150"
     CDS             <1..631
                     /locus_tag="PKJ65_07150"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ41647.1"
                     /translation="SPIKETADTFPTNAYGETKLAVERMLKWASKAYGINYAVLRYFN
                     AAGSHVSGRIGEKRAVETHLIPIALDVALGKIDKLLVFGDDYDTFDGTCIRDYIHVSD
                     LATAHLLALEKMINDSINLERKSGNAGISMIYNLGSEKGFSVNQIIDAVKHVTGVDIR
                     TEITKRRQGDPAVLIASSEKIKKELGWVPEFTDIEDIIATAWEFRKKMR"
     gene            641..937
                     /locus_tag="PKJ65_07155"
     CDS             641..937
                     /locus_tag="PKJ65_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HNZ41648.1"
                     /translation="MNKKLKSKETDYLFDAILSLKNKDECYIFFEDLCTVAEIKAMAQ
                     RLCVAKMLDNKHTYADISVKTGASAATISRVAKCLNYGAEGYTTVLKRLGEING"
     gene            930..>1614
                     /locus_tag="PKJ65_07160"
     CDS             930..>1614
                     /locus_tag="PKJ65_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ41649.1"
                     /translation="MGRILDKIFGTYSDREIKKILPLVEDIEALADEMKGKTDAQLRA
                     YTDVFKQRLKDGETLNDILVEAFAVCREASTRVLHMTPYKVQLIGGIVLHQGRIAEMK
                     TGEGKTLVATLPVYLNALSGKGVHVVTVNDYLAKRDSEWMGKLYNFLGLSVGLIVYGL
                     TKEQRENAYNSDITYCTNNELGFDYLRDNMVTRKEELVQRDLHFAIVDEVDSILIDEA
                     RTPLIISGAG"
BASE COUNT          538 a          278 c          415 g          383 t
ORIGIN      
        1 ttctcccata aaagaaactg ccgacacctt tcccacaaac gcatacggtg aaactaaact
       61 ggcggtggaa agaatgctca aatgggcttc aaaggcttac ggtataaatt atgcggtgtt
      121 gagatacttt aatgcagcgg gaagccatgt aagcggcagg ataggggaga aaagggctgt
      181 ggaaacacac cttataccca tagctctgga tgttgcgttg ggaaaaatag ataaacttct
      241 ggtattcgga gatgattatg atacctttga cggaacctgc ataagagact atatccatgt
      301 cagcgatctg gcgacggcac atcttctggc attggaaaag atgataaatg attctattaa
      361 cttggaaagg aagagcggga atgccggtat tagcatgata tataacctgg gaagcgaaaa
      421 gggtttttcg gtaaatcaga taattgatgc ggtaaagcat gttaccggtg tggatataag
      481 gacggaaata acaaaaaggc gacaaggcga ccccgctgta cttattgcgt ccagcgagaa
      541 gataaagaag gaattgggat gggtacccga gtttacggac atagaggata ttatagccac
      601 ggcttgggag tttcgaaaga aaatgaggtg aaaactaaaa atgaataaga aacttaaatc
      661 aaaagagacc gactatttat ttgatgcaat actttctttg aaaaacaaag acgaatgcta
      721 catctttttc gaggatttat gcaccgttgc ggagattaaa gcaatggctc agcggctttg
      781 tgttgcaaag atgttggata acaagcatac atatgccgat atttcagtaa agacgggggc
      841 ctcggctgct acaataagca gagttgccaa gtgtttgaat tacggagctg aagggtatac
      901 aaccgttttg aaaagattag gagaaattaa tgggtagaat actggataag attttcggta
      961 catacagcga cagagagatt aagaaaatcc tgccgcttgt ggaagatata gaggctctgg
     1021 ccgatgaaat gaagggtaag accgatgcac aacttcgggc atatactgat gtttttaagc
     1081 aacgtctgaa agacggagag acattgaacg atattcttgt ggaagcattt gcggtatgca
     1141 gagaagcttc gaccagagta ctgcatatga ccccgtataa agtacagctt ataggaggaa
     1201 tagtactgca tcagggcaga atagcggaaa tgaaaaccgg agaaggaaag acgcttgtcg
     1261 caacgctgcc ggtttatctg aatgctcttt cgggcaaagg cgttcatgta gttacggtga
     1321 acgactacct ggctaaaaga gacagcgaat ggatgggaaa actgtataac ttcttaggac
     1381 tgtcagtcgg cttgattgtc tacggcctta ccaaggaaca aagagaaaac gcatataatt
     1441 ccgatataac atactgcaca aacaacgagt tgggattcga ctacttaaga gacaacatgg
     1501 tcacaagaaa agaagagctg gtacaaaggg atcttcactt tgccattgtc gatgaagtgg
     1561 acagtatttt aattgacgaa gcaagaacac ctcttatcat atccggagcg ggag
//
LOCUS       DAOPWC010000367         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_85624_length_1612_cov_4.681439, whole genome shotgun sequence.
ACCESSION   DAOPWC010000367 DAOPWC010000000
VERSION     DAOPWC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85624_length_1612_cov_4.681439"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKJ65_07165"
     CDS             complement(<1..243)
                     /locus_tag="PKJ65_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41650.1"
                     /translation="MTKELVLNPKDLRKRSKIKFKDIDVCAYNKTIEEEKKIFSKADF
                     LRIFRDMAIIREFESMLNAVKTTGQYNSVEHAYPGPA"
     gene            complement(311..>1612)
                     /gene="lpdA"
                     /locus_tag="PKJ65_07170"
     CDS             complement(311..>1612)
                     /gene="lpdA"
                     /locus_tag="PKJ65_07170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNZ41651.1"
                     /translation="GKGLNVLLFEKRYLGGVCLNEGCVPSKALLHSAKIYDYTKHSDQ
                     YGIKVTDSTLDQEAVIKRKNKVVRTLVAGVKGTLKRNKVTVVEKVAKIEKKSSDGFVV
                     SAEGELYTADRLLICSGSEPIMPPIPGLHEGYQKGFVLTNREILDLTVIPETLVVVGG
                     GVIGLEMASYFNSAGSKVTVVEMLNKIGGPIDDEISDLLRKEYEKKGIMFNLESKVTS
                     FTDKSVIFEKEGKNVEIECEKVLISIGRRPSTKNLGLENIGVETDKRGAVITDRFGCT
                     NVAEVYAAGDVNGISMLAHTAYREAEVCVNNITGKKDIMRYTAIPAVIYTNPEVAMVG
                     ETEASAGQKNMDFEAVTISMKYSGRYVAENEGGNGICKALREKSTNRLIGVSLIGNYA
                     SELIYGAAMMIESQMTVEDLKELVFPHPTVCEIIREALFAF"
BASE COUNT          391 a          397 c          262 g          562 t
ORIGIN      
        1 tgcaggtccg ggatatgcat gctctaccga gttgtattgt ccggtagttt tgaccgcatt
       61 taacatactt tcaaactcac gtattattgc catatctctg aagatacgta aaaaatccgc
      121 tttggaaaat atcttctttt cttcttcaat tgtcttgtta tatgcacaga catctatgtc
      181 cttgaattta atcttacttc tttttcttaa atctttcggg ttcagtacca gttcctttgt
      241 cattttttaa tctcctttat attcaataac tttttatact ttgcatttta atttcactct
      301 ttgctataca ttaaaatgca aacagcgctt cccttataat ttcgcatacg gtaggatgag
      361 ggaataccaa ttccttcaaa tcttccaccg tcatctgcga ttcaatcatc atggctgctc
      421 cgtaaatgag ttccgaagca tagttgccta taaggcttac acctataagt ctgtttgtgc
      481 ttttttctct caatgcttta catataccgt ttccgccttc attttccgca acatatcttc
      541 cggaatattt catggatatg gtaaccgctt caaaatccat atttttctgt ccggctgaag
      601 cttcggtttc tccgaccatt gccacttcgg gattggtata tatcacggca ggtatggcgg
      661 tatatctcat tatgtctttt ttaccggtaa tgttatttac acatacttcc gcttctctgt
      721 aagcggtatg cgcaagcatg gatatgccgt ttacatcgcc tgcggcataa acttccgcaa
      781 cattagtaca tccgaaacgg tcggtaataa cggcgccccg tttatcggtc tccaccccta
      841 tattctccag tcccaggttt ttggtggaag gtcttctgcc tatgcttatc agcacttttt
      901 cacactcaat ctccacattc tttccctctt tttcgaatat gaccgatttg tcggtaaagg
      961 acgttacttt actttcaaga ttaaacatta ttcctttttt ctcatattct ttacgtaaaa
     1021 gatccgatat ctcgtcgtca atggggccgc ctatcttatt gagcatctcc acaaccgtaa
     1081 ccttgcttcc cgcagagttg aaataggaag ccatttccag ccctataact cctcctccta
     1141 caacaacaag agtttccggt attaccgtaa gatccaaaat ctctctgttg gtaagtacaa
     1201 accccttttg atagccttca tgaagtccgg gaatgggagg cattatgggt tccgaaccgg
     1261 agcatataag cagtctgtcc gccgtataaa gttcaccttc ggcacttacc acaaaaccgt
     1321 cagaagattt tttttcaatc tttgcaacct tttctaccac cgtgactttg tttcttttca
     1381 aagtcccttt tactccggct accaaagttc tgaccacttt attctttctt ttaattaccg
     1441 cctcctggtc caatgtagag tcggtcactt taattccgta ctgatcggaa tgttttgtgt
     1501 aatcatatat ctttgcactg tgaagtaagg ctttggaagg cacacatcct tcatttaagc
     1561 aaactccgcc gagataacgc ttttcaaaca gcagtacatt cagccctttt cc
//
LOCUS       DAOPWC010000368         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_85901_length_1609_cov_3.037967, whole genome shotgun sequence.
ACCESSION   DAOPWC010000368 DAOPWC010000000
VERSION     DAOPWC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85901_length_1609_cov_3.037967"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKJ65_07175"
     CDS             <1..1224
                     /locus_tag="PKJ65_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ41652.1"
                     /translation="IVVVLLIIFLLELLLYSLFRRFVYKKIPYFYEEPLVDDVKEKGV
                     NPLEDKKEVTLNKDVEKIIALIPEITIESDDVEDKIKEVKHRFSENTVFIESSNPEEI
                     LRSRLKSPHLVVHLCSLNKVRHINTLLSYANYCMNEGGFIACHCVTSALHREKVLRQS
                     PPIINRVVFGLDFFWHRVCSKIPVTRPLYFWITGGKSRVLTRVEVLGRIYRAGFDVVH
                     EEISNGCFYVVATKIKEPIRDDKPTNGMLIRLQRQGRGGEVIGVYKLRTMYAYSEYIQ
                     PYIYKQQGLAVGGKISDDYRVSPLGHFLRKTWLDELPMLINWVKGELKIVGVRPLSSH
                     YFNLYDEDLKALRIKTKPGLLPPFYADMPETLEEVQESERRYLNAYFKNPISTDWKYF
                     WKAVRNIVVKGKRSQ"
     gene            1261..>1609
                     /locus_tag="PKJ65_07180"
     CDS             1261..>1609
                     /locus_tag="PKJ65_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ41653.1"
                     /translation="MKGIVLAGGSGTRLYPITKGISKQLLPIYNKPMVYYPISVLMLA
                     GIRDILVISTPQDLPGFKRLLGDGSDYGVHFEYAEQPSPDGLAQALIIGEEFVGDDAV
                     CLVLGDNIFYGQGF"
BASE COUNT          482 a          276 c          388 g          463 t
ORIGIN      
        1 attgttgtag tattactgat tattttcctt ctggaactat tactttattc ccttttcaga
       61 agatttgtat ataaaaagat accttatttt tacgaagaac ccctcgttga tgatgttaaa
      121 gagaaagggg taaatccttt ggaagataag aaagaggtca ccctaaacaa ggatgttgaa
      181 aagattattg ctcttatccc tgaaataacg attgaaagtg acgatgtaga agataaaatt
      241 aaagaggtta agcaccggtt ctcggagaat accgtattca tagaaagcag caatccggag
      301 gagattcttc gttccaggct taagtcccct cacttagtgg tacatctctg ttctttaaac
      361 aaagtcaggc atatcaatac cctgctttca tacgcaaact actgtatgaa tgagggagga
      421 ttcatcgctt gtcattgtgt gacctcagca ttgcatcgcg aaaaggtttt acgccaaagt
      481 ccccccataa ttaacagagt agtttttggt ttggatttct tctggcaccg ggtatgttcc
      541 aaaattccgg ttacacgccc gttatatttt tggattacgg gtggaaagtc ccgggtgctg
      601 acacgtgtag aggtattggg aaggatatat agagcaggat ttgacgtggt acatgaggaa
      661 ataagtaacg ggtgctttta tgtggtggca acaaagataa aggagcctat tcgtgacgat
      721 aagcctacaa atggcatgtt gattcgtctg cagagacagg gaaggggagg cgaggtcata
      781 ggtgtctata aattaaggac tatgtatgcc tattccgaat atatacaacc atatatatat
      841 aagcagcagg gacttgcggt tggcggaaaa atttcagatg attaccgtgt cagtcctctc
      901 ggtcattttt tgcgtaaaac atggctggat gagctgccca tgctgatcaa ctgggtaaaa
      961 ggtgaactta aaattgtggg agtaaggcct ctaagcagtc actacttcaa tctttatgat
     1021 gaggacttaa aagcattaag aataaaaacc aagccgggac ttctgccacc attctatgca
     1081 gacatgccgg aaacccttga ggaagttcag gaaagtgagc ggcgatattt aaatgcatat
     1141 ttcaaaaacc cgattagtac agactggaaa tacttttgga aagcggtacg taatattgtg
     1201 gtgaaaggga agaggagtca gtaagaaatg attgattaaa aaacatatac attattagct
     1261 atgaagggaa ttgttcttgc cggtggatca ggtacccgct tatatccgat tacaaagggt
     1321 ataagtaaac aactcttacc catttataac aagccgatgg tttattatcc aatatcggtg
     1381 ttgatgttgg cggggattcg cgatatattg gtaatttcca cgcctcagga tctgccggga
     1441 tttaaacgtc tcctgggtga tggctcagac tacggtgtgc attttgagta tgcggaacag
     1501 ccgagtccag atggactggc gcaggcctta attataggtg aagagtttgt aggggatgat
     1561 gcagtatgcc ttgttttagg agataatatc ttttatggac aaggttttc
//
LOCUS       DAOPWC010000369         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_86341_length_1604_cov_2.594577, whole genome shotgun sequence.
ACCESSION   DAOPWC010000369 DAOPWC010000000
VERSION     DAOPWC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86341_length_1604_cov_2.594577"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKJ65_07185"
     CDS             complement(<1..1421)
                     /locus_tag="PKJ65_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ41654.1"
                     /translation="MIHPIQYGKRERMSFSKIKEVLEMPNLIEVQKKSYQWFLDEGLR
                     EVFKDVSPVRDHTDNLMLDFIDYRIADKPKLDVQECKERDATYQAPLRVKVRLIRKDT
                     GEVKEQEIFMGDFPIMTDTGTFIINGAERVIVSQLVRSPGAYYAEKKDKAGKSLFSST
                     LIPNRGAWIEYETDSNGVLYVRIDRTRKLPLTVFLRALGFAKDSDITAVFGEDYKLDA
                     TYAKDETKTREGGLFEIYKRLRPGEPPTIESAENLFEGLFFDARRYDLARFGRYKFNK
                     KLSVAPRIAGTVSAERIVDSETGEILVEADEVIDEAKAIAVENAGINVVKVKCDNHVH
                     VVIGNETVDASSFIDFNHTALGLTDKVNYKVLKEILAECSTQEEIKEAFEKRRYDLCP
                     KNITRIDIIASISYLLGLDYGLYHTDDIDHLGNRRIRSVGELLQNQFRIGFARMERVI
                     REKMTIQDIDIVTPQALINIRPVT"
BASE COUNT          405 a          389 c          270 g          540 t
ORIGIN      
        1 gttacgggac ggatattaat caatgcctga ggtgttacaa tatcaatgtc ctgaatggtc
       61 atcttctcgc gaattacccg ctccattctt gcaaacccta tcctgaactg attctgcagc
      121 aattctccta ccgatcttat tcttctgtta cccagatggt ctatgtcgtc ggtgtggtac
      181 aaaccgtaat ccaatcccag taagtaactt atggatgcta taatatcaat cctggtaata
      241 tttttgggac ataagtcata tcttcttttt tcaaaagctt cttttatttc ttcctgtgtg
      301 gaacattcgg caagtatttc tttcagtact ttgtagttta ccttatcggt aagccccagt
      361 gcggtatgat tgaaatctat gaaagaagac gcatcaaccg tctcatttcc tataactaca
      421 tgaacatgat tatcacattt tacttttact acatttattc cggcattttc caccgcaatt
      481 gccttagcct cgtcaataac ttcatcggct tccaccaaaa tctctccggt ctcggaatca
      541 actattctct cggctgatac agttcctgct attctcggag caacggagag tttcttgttg
      601 aatttatatc ttccgaatct ggcaagatca tatcttctgg catcaaaaaa caatccttca
      661 aataagtttt cggcactttc tatcgtagga ggctcgcccg gtcttaacct tttgtatatc
      721 tcaaacaatc cgccttctct ggttttcgtt tcgtcttttg catatgttgc atccagttta
      781 tagtcttctc cgaataccgc ggttatatcg gaatctttag caaatcccaa tgctcttaag
      841 aaaaccgtca gaggcaactt tctggttctg tctattctta catacaaaac cccgttggaa
      901 tcggtttcat attctatcca tgctcccctg ttgggaataa gtgtgctgga aaaaagactt
      961 tttccggctt tatccttctt ctccgcataa tatgcaccgg gagatcttac caactggctg
     1021 acaataactc gctccgcacc gttaatgata aatgtgccgg tgtcggtcat tatggggaaa
     1081 tctcccataa atatttcctg ttccttgacc tctccggtat cttttcttat aagacggact
     1141 tttactctca aaggagcctg ataggtggca tctctttcct tacattcctg cacgtcaagc
     1201 ttaggcttgt ccgcaatcct gtaatcaata aaatccagca ttaaattatc ggtatgatca
     1261 cgaaccggtg atacatcctt aaaaacctct ctgagtccct catcgagaaa ccactgataa
     1321 gactttttct gaacttctat aagattgggc atttccagaa cttctttgat ttttgagaaa
     1381 ctcattcttt ctcgttttcc gtattgaata ggatgtatca ttaattagtc acctcatgtc
     1441 cttttctata tcatccccat ggtaacggct gctgttttaa gtgtcggcaa accggtgcca
     1501 taataacctt atgcacctcc gatttcagta atttcctgtc gacaggcgca aaaatacgca
     1561 gattagcgca tcattcataa tcctatcgca tatgtctgcc cgtg
//
LOCUS       DAOPWC010000370         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_87716_length_1588_cov_2.911285, whole genome shotgun sequence.
ACCESSION   DAOPWC010000370 DAOPWC010000000
VERSION     DAOPWC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87716_length_1588_cov_2.911285"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1464)
                     /gene="amrA"
                     /locus_tag="PKJ65_07190"
     CDS             complement(136..1464)
                     /gene="amrA"
                     /locus_tag="PKJ65_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNZ41655.1"
                     /translation="MSVAAAFIFPHPPLILPKIGKGEEKKIQKTIDSYKEACKTIAQL
                     QPDTVVLASSHCAMYSDYLHISDGEYYQSDMGRFGAASISVRAEYDKQFRDKLMEIFS
                     EQNIPAGTQGQEIPGMDHASVIPLVFLNEVYRDYKLIRMGISLLPFETHYKLGMCVAK
                     ASELLGRRVVFIASGDLSHKLKVTGPYGYSKQGEQYDQMVTEAMTKGNFAKFMDFSEE
                     FLEKAAECGHRTFVIMAGALDKKTIEPKLLSYENTFGVGYAVASFLVEQKEKEVTSDM
                     YVALAKSSVEYYVKNKRMLKIPPDLPAELTEKRAGVFVTLKKDGDLRGCIGTISPVTG
                     NIAEEIIRNAVSSCSHDPRFYPVREEELDALEYSVDVLKEAERIDSIDQLDVNKYGVI
                     VTSGYRRGLLLPNLEGVDSVQQQLAIALSKAGIASGEKFEMERFEVVRHR"
BASE COUNT          404 a          398 c          289 g          497 t
ORIGIN      
        1 ggtccagtgc tatggatgtg agtttgccgt agttttcgca tatcatcttt ccatccttat
       61 tcatcctggc acggcacaac ccgacctgcc cttccgaaag catacaatta ttcggacata
      121 tgggacattt aatcttcatc tatgccttac tacctcaaat ctctccattt caaatttctc
      181 tccgctggct atacctgcct tactcagcgc tatcgccagt tgttgctgca cgctatccac
      241 tccctccagg ttgggaagca gtaatcctct tctgtatccc gatgtaacga tgaccccgta
      301 cttattcacg tccagttggt ctatgctgtc aattctttcc gcttctttca gtacgtcaac
      361 actgtattcc aatgcgtcca actcctcttc acgtacggga tagaacctgg gatcgtgaga
      421 acaggaactg accgcattcc ttattatctc ttccgcaatg ttcccggtaa cgggcgatat
      481 tgttcctatg catcctctca aatcaccgtc ttttttcagt gttacgaaca caccggcacg
      541 tttttctgtc agttcagccg gcagatcggg cggaatcttg agcatacgtt tattcttcac
      601 ataatactcc acggaggatt ttgccaaagc cacatacata tccgatgtaa cttctttttc
      661 cttttgttca acaagaaaag atgctactgc atatcccaca ccgaaagtgt tttcgtaaga
      721 caagagtttc ggttctattg tttttttatc cagtgcaccg gccataatta caaaggtacg
      781 atgtccgcat tcggcggctt tttccagaaa ctcctccgag aaatccataa atttcgcgaa
      841 atttcccttt gtcatggctt cggtaaccat ctgatcatac tgttctccct gctttgaata
      901 tccgtacggt ccagtcactt tcagtttatg cgataaatca ccgcttgcta tgaaaacaac
      961 acgccttccg agcaattccg aagcctttgc aacacacata cccaacttgt agtgggtttc
     1021 aaaaggcaga agggatattc ccatccttat aagcttataa tctctatata cttcattcag
     1081 aaaaaccaac ggtattacgc ttgcatggtc cattccgggg atttcctgac cttgggtacc
     1141 tgccggtata ttctgttcgg aaaaaatttc cataagttta tctctgaact gcttatcata
     1201 ttccgcccgc accgatatag atgcggcacc gaatcttccc atatccgatt gatagtattc
     1261 accgtcggag atatgtaaat agtcggaata catggcacaa tgcgaggatg ccagaacaac
     1321 agtatcgggt tgcaactgtg ctatggtttt acaggcttct ttatatgagt ctatggtctt
     1381 ctgtatcttc ttctcttcac cttttcctat cttcggaagt ataagcggag gatgcggaaa
     1441 aatgaatgct gctgctactg acacaataca cctcctattt tgctttgatt atgaaaaaat
     1501 tataccgtaa aacggtgttt tattcaacaa gccgtcaccg ttcttagtgt aaagtatttt
     1561 ggggaaaata aatttaaaag ttaataaa
//
LOCUS       DAOPWC010000371         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_87856_length_1586_cov_2.924233, whole genome shotgun sequence.
ACCESSION   DAOPWC010000371 DAOPWC010000000
VERSION     DAOPWC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87856_length_1586_cov_2.924233"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..841
                     /locus_tag="PKJ65_07195"
     CDS             107..841
                     /locus_tag="PKJ65_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41656.1"
                     /translation="MELYIENLSKYYGEKLALDKFTYTFKEGTYAILGPNGAGKSTLM
                     HLITDNISRSGGQIFYDGKDILQLGRSFRKKIGFMPQSQGMYDQMSAKGFLSYMAEIK
                     GIPKKEARGVINELLSVVNLSDVGHKKLSSFSGGMRQRILLAQALLGKPDILMLDEPT
                     AGLDPEERVRIRTYIGNLAASKIVLITTHITSDVESIADYVIFMKNGRIEASGKPERI
                     LKETCSENMEEAYLKIIHTEGQNAGT"
     gene            828..>1586
                     /locus_tag="PKJ65_07200"
     CDS             828..>1586
                     /locus_tag="PKJ65_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41657.1"
                     /translation="MRELKRIIVSKTNIVCIAFLIFMNVFIFFWESTPKGDICTLPEY
                     TDRYIEVLNTCKGMDNEEGINYLNNLKKLSEGTVLIPRWLYSKDMEYKEEASGFYYKK
                     YGEQMIKDIESGKIDTSEQAVKKAYVTIEVCNILIEKINHLTEYPEYIKKISENAENM
                     KSVSIFSDPNSFSYRNIIKTVEDFSKIHITELSLANDLAVNALFTDSISDYSILAYII
                     FIVLLMIQERKKGLWPLVYAYPEGRARLAIKRVAI"
BASE COUNT          555 a          288 c          331 g          412 t
ORIGIN      
        1 gataataaac cggattacta tatagacaaa tccgagatag gaaccgataa gtttacgttt
       61 cataaaatag agaattagtt atggcaggta atgaaaggag cttcatatgg aactttatat
      121 agagaatctt tccaagtact acggagagaa acttgctctg gacaaattca cttatacctt
      181 taaggaagga acatatgcga tattaggtcc caacggtgcg ggaaaaagca cccttatgca
      241 ccttataacc gacaacatat cccgctcggg cggacagata ttttatgacg gtaaggatat
      301 attgcagttg ggcagatcct tcagaaaaaa gataggattt atgcctcagt cacagggcat
      361 gtatgaccag atgagtgcca aaggtttttt aagttacatg gcggaaataa aagggatacc
      421 taaaaaagaa gcaagaggtg ttattaatga gcttttgtcc gttgtcaatc tttccgatgt
      481 aggtcataag aagctttctt ccttctcggg aggtatgcgt caaagaatac ttttagcgca
      541 ggccttactg ggtaaaccgg acatactgat gctcgatgaa cctacagccg gattggatcc
      601 cgaggaaagg gtaagaataa ggacatatat aggtaatctt gccgcatcaa aaatagtact
      661 cataaccacc catattacat ccgatgtgga atccatagcc gattatgtaa tattcatgaa
      721 aaacggacgc atagaagctt cgggaaaacc cgaacgaata cttaaagaaa cttgctctga
      781 aaacatggaa gaagcatatc tgaagataat acatacagag gggcaaaatg cgggaactta
      841 agcgaatcat agtatcaaaa accaatattg tctgtatagc ttttctgata tttatgaatg
      901 tctttatctt tttctgggaa tcaacaccca aaggagatat atgcacatta ccggaatata
      961 ccgaccgcta tatcgaagta ttgaatacct gcaaaggcat ggataatgaa gaaggcatca
     1021 attatctgaa caatctgaag aaattatcgg aaggaacggt attaattccc cgttggttat
     1081 acagcaagga tatggaatac aaggaagaag cttccggttt ttattacaaa aaatacggcg
     1141 agcagatgat aaaagacata gaaagcggca agattgacac ttcggaacaa gcggttaaga
     1201 aagcatatgt taccattgaa gtatgcaata tacttatcga gaagataaat catctgacag
     1261 aataccccga atatatcaaa aaaataagcg aaaatgccga gaatatgaaa tcggtaagca
     1321 ttttttccga tccgaattcc ttttcctaca ggaatattat taaaacagta gaggattttt
     1381 ccaagataca tattaccgaa ctgtcattgg ctaatgacct tgcggtcaat gccctgttta
     1441 ccgacagtat ctccgattac agcattctgg catacattat cttcatagtc ctgctcatga
     1501 ttcaggaacg taaaaaaggc ctctggccgc ttgtatatgc ctatcctgag ggaagagcaa
     1561 ggcttgcaat caaaagagtt gctata
//
LOCUS       DAOPWC010000372         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_87965_length_1585_cov_2.750980, whole genome shotgun sequence.
ACCESSION   DAOPWC010000372 DAOPWC010000000
VERSION     DAOPWC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87965_length_1585_cov_2.750980"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKJ65_07205"
     CDS             <1..330
                     /locus_tag="PKJ65_07205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ41658.1"
                     /translation="EDSSKFVPVMILEALPSTQGIYGFVVAFLIIGKVGGLSLEQGFS
                     LIIAGLPAGVVGLISAIFQGQVAVASIGIVAKRSADMVKGVVLTLMVELFAIFALLIS
                     ILMVGKV"
     gene            331..936
                     /locus_tag="PKJ65_07210"
     CDS             331..936
                     /locus_tag="PKJ65_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNZ41659.1"
                     /translation="MPGIDRIYDKIIADAEERAGLRLSETSSKIQSMKSVVDERIEEY
                     RKVKISETEKEAKGIVSRAQANRRLEGKKQLLAVKQEMVTEVFEKTQQALINLSNAEK
                     LTLYTKLIGNCLKEGHNEVSLNGKDKKAFGDKLIQNIGSALGPDISVALSGQDIPDDF
                     GVIVKNGKIYSNSTFSSMLKYMKQNIEADVMKILFKRGVEV"
     assembly_gap    1071..1080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1099..>1585
                     /locus_tag="PKJ65_07215"
     CDS             1099..>1585
                     /locus_tag="PKJ65_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNZ41660.1"
                     /translation="MTMLYNEQKSLYAYMREILPDTKIFDAFFLKFDIHNLKVLLKAE
                     FLKKPYDEIALDTGVYKYEELKEWLHNRDFSHLPEDIAQAVQLSMEEFLKTNDPQLID
                     LIMDKAYYTYFNRIMEKTYDRFLKDFTIIQTDLMNLKAFMRIKKVFRKEQFLNYAIIP
                     GG"
BASE COUNT          522 a          278 c          371 g          404 t
ORIGIN      
        1 gaagattcca gcaaattcgt gccggtaatg attttagaag cgctgcccag cacgcaggga
       61 atttacggtt tcgttgttgc cttcctcata atcggtaaag tcgggggctt aagtcttgaa
      121 cagggattca gcttaataat tgccggcctg cccgcaggtg ttgtcggcct tatttccgca
      181 atattccagg ggcaggtagc cgtggcgagt ataggcattg ttgcaaaaag aagcgctgat
      241 atggttaagg gcgtagttct tacacttatg gtagagcttt ttgctatatt cgcactgctc
      301 atatccatac tgatggtagg taaagtataa atgccgggaa tagacaggat atatgacaag
      361 ataatagctg atgccgaaga acgtgcgggg ttacggttat ccgaaacctc ttccaaaata
      421 caatcaatga aatctgttgt ggatgaaaga attgaggaat atcggaaggt taaaatatcc
      481 gagacggaaa aagaagcaaa gggcattgta tcccgggcac aggccaacag gcgccttgag
      541 ggtaaaaaac agttgcttgc ggttaagcag gaaatggtaa ccgaagtgtt tgaaaaaaca
      601 caacaggcac tgataaacct tagtaatgcc gagaaactga ctttgtatac aaagcttatc
      661 ggcaactgtc ttaaagaggg acacaatgag gtaagcttga acggtaaaga caaaaaggct
      721 ttcggtgaca agcttataca gaatataggg agtgctttag gtccggacat ttcggttgct
      781 ctttccgggc aggatatacc cgatgatttc ggagtaatag taaaaaacgg aaaaatatac
      841 tccaactcca cattctcatc aatgctcaag tatatgaagc agaatataga ggcggatgtt
      901 atgaagatac tttttaaaag aggtgttgag gtttgagaaa ttccgacaaa tatgcacact
      961 ccataggaag agtgcgcgta agagaaaact tcatgatgga tgaacaaaag cttgcaaggc
     1021 ttgtggatgc tcccgacatg aaagaagcgg tcaggattct tgccgagtgc nnnnnnnnnn
     1081 gttgaagatg aggattatat gactatgttg tataacgaac agaaatcact ttatgcatat
     1141 atgagagaga ttttgcccga cacaaaaata ttcgatgcct ttttccttaa gttcgacata
     1201 cataatttaa aggtattgct taaagcggaa ttcttaaaaa aaccttatga cgagatagcg
     1261 ctggataccg gtgtgtataa atatgaggaa cttaaagaat ggcttcataa cagagatttc
     1321 tcacaccttc cggaagatat tgcacaagcg gtacagctca gtatggaaga gtttttgaaa
     1381 accaacgatc ctcagctgat tgatctcata atggacaaag catactatac atacttcaac
     1441 agaataatgg agaaaacata cgacaggttt ttgaaggact ttacgataat acagaccgat
     1501 cttatgaact taaaggcgtt tatgaggatt aaaaaagtat tccgcaaaga acaattctta
     1561 aactatgcca tcatacccgg aggat
//
LOCUS       DAOPWC010000373         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_88825_length_1575_cov_3.521711, whole genome shotgun sequence.
ACCESSION   DAOPWC010000373 DAOPWC010000000
VERSION     DAOPWC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88825_length_1575_cov_3.521711"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1575
                     /locus_tag="PKJ65_07220"
     CDS             <1..>1575
                     /locus_tag="PKJ65_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ41661.1"
                     /translation="NKSFLDFLVKIKNLFVKFFHWMKDAFKPIEEQEEEPEPAKSKKQ
                     QYVQGTINDIAEPVKETISGEPANIIINKSKPVKEETKSTVPKYTGPIIIKKEEPVKE
                     ASVTTEKPQVRGEYVFPPLSLLDSYVDSGNTDNHEQIARKLENTLLSFGVEVTVTNIM
                     CGPTVTRYELQPKAGVKVSRIVALADDIALNLASRDVRIEAPVPGKSVVGIEVPNTKK
                     SVVSLKEVIASKEFQGSKSLLTIAIGKDIAGNCTVADVRRMPHMLIAGATGSGKSVCI
                     NCIITSILYKATPDDVRFIMIDPKKVELINYNGIPHLLIPVITNLKKAAGALNWAVEE
                     MTNRYDMFNKRGVRDINTYNDSVKGDPQLKKLPNIVIIIDELADLMMSNPGEVEESIC
                     RLAQLARAAGIHLIIATQRPSVNVITGVIKANIPSRLSFAVVSQVDSRTILDMGGAEK
                     LLGNGDMLFHPMGLSKPVRMQGAYISDAEINKVVEFIKQRTSATYDEDVVENIEKADK
                     KGSDSDMENEVDELLPA"
BASE COUNT          526 a          311 c          374 g          364 t
ORIGIN      
        1 aaacaaaagt tttctggatt ttctggtaaa gataaaaaac ttatttgtca agtttttcca
       61 ctggatgaaa gatgcattca aacccattga agagcaggaa gaagagcctg aacctgccaa
      121 atctaaaaaa caacagtatg tacaaggcac gattaatgat attgccgaac cggttaaaga
      181 aacaataagc ggagagcctg caaatataat aatcaacaaa tccaaacccg taaaggaaga
      241 gaccaagagt acggtaccta aatataccgg tccgataatt ataaagaaag aggagccggt
      301 aaaagaagct tcggtaacga cggaaaagcc tcaggtcaga ggtgagtatg tatttcctcc
      361 gctgtcgctt ctggattcct atgtggacag cggtaatacc gataatcatg aacagatagc
      421 aagaaagctg gaaaatacgt tgctgagttt cggtgtggaa gtaacggtaa ccaatatcat
      481 gtgcggtccc accgtaacaa gatatgagct gcaacccaaa gccggtgtaa aagtcagcag
      541 gatagtggct ttggccgatg atatagcctt aaaccttgct tcacgggatg tgaggataga
      601 agctccggtg ccgggaaaat cggtagtggg tatagaagta cccaatacca aaaagagtgt
      661 ggtatcgctt aaagaagtta ttgcttccaa agagtttcag ggttcaaaaa gccttcttac
      721 cattgcaata ggtaaagata ttgccggcaa ctgtacggtt gccgacgtca gacgcatgcc
      781 tcatatgctt atagccggtg caaccggttc gggaaaaagc gtatgtatca actgtattat
      841 taccagtatt ttatacaaag ccactcccga tgatgtgcgc tttataatga tagaccctaa
      901 gaaagtggaa ttgattaatt ataacgggat accgcatctg ttgattccgg taatcaccaa
      961 tcttaaaaaa gccgcgggag ctttgaattg ggcggtagag gaaatgacca acagatatga
     1021 catgttcaat aaaaggggag tgcgcgatat caatacatac aacgactcgg taaaaggcga
     1081 tccgcaactg aaaaaactgc ccaatatcgt gataataatt gacgagctgg ccgaccttat
     1141 gatgtccaat ccgggcgaag tggaagaatc catatgcaga ctggcacaac ttgccagagc
     1201 tgccggcatc catcttataa tcgcaacaca gcgaccttcc gtcaatgtca taaccggtgt
     1261 tatcaaagca aacattccgt ccaggctttc ctttgcggtg gttagtcagg tagactccag
     1321 aaccatattg gatatgggcg gagcggagaa acttttagga aacggagata tgctattcca
     1381 tcccatgggc ttatccaaac cggtcagaat gcagggcgca tatatatccg atgccgagat
     1441 aaacaaggtg gtggaattta ttaaacaaag aacctctgca acttatgatg aagatgtagt
     1501 ggaaaatata gaaaaagcgg acaaaaaagg ttcggacagc gacatggaaa atgaagttga
     1561 cgaactgttg cctgc
//
LOCUS       DAOPWC010000374         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_88981_length_1573_cov_4.013175, whole genome shotgun sequence.
ACCESSION   DAOPWC010000374 DAOPWC010000000
VERSION     DAOPWC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88981_length_1573_cov_4.013175"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKJ65_07225"
     CDS             <1..1249
                     /locus_tag="PKJ65_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNZ41662.1"
                     /translation="TYFDEIYFPRTAYEFLIKNQTIYENTHPHLGKIIIMWSYKMFGV
                     NPFGYRAMGAIAGALMIPVMYALGKKLFKNHYFAMIGATLLAFECMHFVQTRLGTVDS
                     YLVLFIMLTFYFMACYVDMDLRKTSYFKTLVPLFFSGIFFGCAISVKWIGFYAGAGIA
                     FLFFVKIFSQWKSFGYGRIVYGGKALAARNSLLTCFFCLIFFIAIPALIYSLSYIPIF
                     QIQSAAIDNEKFTIMEMADNIIASQKHMFEYHKGVTQDHPFKSAWYTWPIMYKPVYYY
                     SAPVKAEGQWGTIVAIGNPLIWWTGLAALIGTAIVSLRKQDRRGLFIFAGYLSILLPW
                     AVAPRSITFLYHYFGCVPFMILAIVYMASYFMEDNPKVKKYINAYVILAILIFAAFYP
                     AMAGIKVSVDYIELLRWLPSWWF"
     gene            1250..>1573
                     /locus_tag="PKJ65_07230"
     CDS             1250..>1573
                     /locus_tag="PKJ65_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HNZ41663.1"
                     /translation="MRELYDVSASEHNPKWENMICRKEHIGRKEQDIRSEFYRDYTRI
                     INSRAYRRLKQKTQVFFATRNDHVCTRMEHVNNVLSISYGAAKYLGLNEELVMAIAVG
                     HDLGHS"
BASE COUNT          441 a          303 c          365 g          464 t
ORIGIN      
        1 cacctatttc gacgagatat attttcccag aacggcatat gagtttttaa taaaaaacca
       61 aacaatttat gaaaatacgc atcctcactt aggtaagatt ataatcatgt ggtcctataa
      121 aatgttcggg gtaaatcctt tcgggtacag agctatggga gcaatagcgg gagcacttat
      181 gataccggtt atgtatgctc tgggaaagaa gttatttaag aaccattact ttgctatgat
      241 aggggcaacg cttttagcgt ttgaatgtat gcatttcgtg cagacaagac tgggaacggt
      301 ggacagctat ctggttcttt ttataatgct taccttctat tttatggcat gttatgtgga
      361 tatggatttg cggaaaacaa gctacttcaa aacgcttgtg cctttattct tctccggtat
      421 tttcttcgga tgtgccatat cggtcaaatg gataggattt tatgcgggag cgggaattgc
      481 gtttctgttc ttcgttaaga tattttcaca atggaaatct ttcggatacg gacgcattgt
      541 atacggaggc aaagcacttg ccgcaagaaa cagtctgctt acctgcttct tctgtctgat
      601 tttctttatc gctataccgg cccttatata ttcactgtca tatattccta tctttcagat
      661 acagtcggcc gctatcgaca atgaaaagtt tacaataatg gaaatggcgg ataacataat
      721 tgccagccag aagcacatgt ttgaatacca taagggagtg acacaggacc atcctttcaa
      781 atccgcctgg tacacatggc ccatcatgta caagccggtt tattactact cggctcccgt
      841 aaaagccgaa gggcagtggg gcacaatagt ggcaataggc aatcccttga tatggtggac
      901 cggtcttgcg gcgcttatag ggacggcgat agtgtcgtta agaaagcaag acagaagagg
      961 actgttcata tttgcaggct atctttccat actgttgccg tgggccgtgg cacccagaag
     1021 cattaccttc ctgtatcatt atttcggatg tgtgcctttt atgattctgg caattgtgta
     1081 tatggcctca tactttatgg aggacaatcc caaagtgaaa aaatatatta atgcatatgt
     1141 aatattggct atccttatct ttgcggcttt ttatcccgca atggccggga taaaagtcag
     1201 cgtcgattat atcgagcttt taagatggct tcccagctgg tggttttaaa tgagagaatt
     1261 atatgatgtg tcggcttcgg aacataatcc gaaatgggag aatatgattt gccgtaaaga
     1321 gcatatcggc aggaaagagc aggatatacg ctcggagttt taccgggact ataccaggat
     1381 aataaacagc agagcttaca gaagacttaa gcaaaagacg caggtgtttt ttgcaacgag
     1441 gaatgaccat gtctgcacca gaatggagca tgtgaataat gtgttatcca tcagttacgg
     1501 tgccgcaaaa tatctcggac ttaatgaaga acttgtcatg gcgattgccg taggtcatga
     1561 tctgggacac tcg
//
LOCUS       DAOPWC010000375         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_89361_length_1569_cov_3.132100, whole genome shotgun sequence.
ACCESSION   DAOPWC010000375 DAOPWC010000000
VERSION     DAOPWC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89361_length_1569_cov_3.132100"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKJ65_07235"
     CDS             <1..560
                     /locus_tag="PKJ65_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41664.1"
                     /translation="EYDTNSYMASNQYYIFPEIVGSEATLNVYGNISYLLTPNARSIR
                     LLNKSKAFLSVNPLLRTSSLSKQEASISGYDSTSGAAYVGASARSLATESRLVVLGST
                     DFMRDYILVNNKSYEADSSKFFIGLLNWLEGDYSRVNIEEKNYFVNILSVTASQANFV
                     AVLLYVLPALILLTGGIVYLKRRHL"
     gene            561..>1569
                     /locus_tag="PKJ65_07240"
     CDS             561..>1569
                     /locus_tag="PKJ65_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNZ41665.1"
                     /translation="MKNLKTIIIIVIILAVAVAAYFIIDDIIAKTRVENPKITEIITP
                     VNIKSDDIMRYTFCISGEQTTVELMEVKVKDEQGEEKTEMQYRLVNEPERELNQNIST
                     ALVQAASLISVDVIEESPKNLAKYGIDYNSYFEVTLKNGTSYKVYFGDVIDVTYNVYA
                     MREGVDKVYTISDTSFGMLTIYREYLLSEVIFPGSANSLTSFSLYKKGELEFTLEPDE
                     HIKWKLVEPLSAKTYSQTAQEMVDKTYEMVIGEYVNVLPTEADYRNYDLFKPAYSIKV
                     TADKKTVTLHIGRQSIEDRSYYAKFDNSEEVFLVPTKYLGWIDTELLEILYPFPYEPL
                     IK"
BASE COUNT          487 a          323 c          340 g          419 t
ORIGIN      
        1 aggaatatga caccaactcc tatatggcca gcaaccagta ctatatcttc cccgagattg
       61 tgggctcgga ggccacttta aatgtgtacg gcaatatctc ctatctgctt acccccaatg
      121 ccagaagtat aaggctgtta aataagtcca aagcattttt gtcggtaaat ccgttattga
      181 ggacaagctc tttatccaag caggaagctt ccataagcgg gtatgattcc acatcgggag
      241 cggcatatgt aggagcttcg gcaagaagcc ttgcaaccga atcgcgattg gttgttttag
      301 gttccaccga tttcatgaga gactacattc ttgttaacaa caagtcatat gaagccgatt
      361 cgtcaaagtt cttcataggt cttttaaact ggctggaagg cgattacagc cgggtcaaca
      421 ttgaagaaaa gaattacttt gtaaatatat tatccgttac cgcatcgcag gctaatttcg
      481 tagcagtgct tctttatgtg ctgcctgcct taatcctgtt aacaggcggt attgtttacc
      541 ttaagaggag gcacttataa atgaagaatc tgaagaccat catcattata gtaataatat
      601 tggctgttgc agtggcggct tactttataa tcgatgacat tattgccaaa accagagtgg
      661 aaaatccgaa aataaccgag attatcactc cggtaaatat taaatccgac gacataatgc
      721 gttatacatt ctgtatttcg ggagagcaga ccaccgtgga actcatggaa gtgaaagtaa
      781 aagatgagca gggcgaggaa aaaaccgaaa tgcagtacag actggtaaac gaaccggaaa
      841 gagaactgaa tcagaatata agtacggcac ttgtacaggc tgcatcgctt atcagtgtgg
      901 atgttattga ggaatctccg aagaatcttg ccaagtacgg gattgactac aactcgtatt
      961 ttgaagttac ccttaaaaac ggcacaagct acaaagtgta tttcggtgat gtaattgatg
     1021 tcacatacaa tgtgtatgca atgcgtgaag gagtggacaa ggtctatacc atttccgata
     1081 cctccttcgg tatgcttacc atttacagag agtatcttct ttcggaagtt attttccccg
     1141 gcagtgccaa cagtcttaca tccttttcac tttataaaaa aggtgagctg gagtttacac
     1201 tggagccgga tgaacacatt aaatggaagt tagtggagcc gctgtccgca aagacctatt
     1261 ctcagacagc tcaggaaatg gtggataaga catatgagat ggttatagga gaatatgtga
     1321 atgtactccc taccgaagcc gattacagaa actatgacct tttcaaaccc gcatactcca
     1381 taaaagttac cgccgacaaa aagaccgtga cactgcatat aggcagacag tccatagaag
     1441 acagaagcta ctatgccaag tttgacaatt ccgaagaagt attccttgtt ccgaccaaat
     1501 atctgggatg gatagatacc gaacttttgg aaatactcta tcccttcccg tatgagcctc
     1561 ttatcaaga
//
LOCUS       DAOPWC010000376         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_90484_length_1557_cov_3.478695, whole genome shotgun sequence.
ACCESSION   DAOPWC010000376 DAOPWC010000000
VERSION     DAOPWC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90484_length_1557_cov_3.478695"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKJ65_07245"
     CDS             <1..931
                     /locus_tag="PKJ65_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41666.1"
                     /translation="QIHNQMVNHEKAQVTAISMIDREKLPKELLTGDVVFYENFDSML
                     KAKNVDVISICAPRRIDQKDLAVKALLAGKHVYSEKPCAYTEKDLDEILEVSKKTGML
                     FHEMAGSAFEEPFISVTELIKSGRIGEVVQVFAQKSYPFKTSAGRPQDDITDGGLIRW
                     IAVHAARFVEHTTGLKIKDIYAMETRHGNPVKDGNLKMAASIMFSLDNNAVGCIIANY
                     LNMNNFPTWGNEHLRVWGTDGFVEITDGLTATRLVTPDYDGPVEIKYATADYFDFFID
                     EILYNKPMPLTLEEELHPLRAVIRAQEKAVEVQ"
     gene            931..>1557
                     /locus_tag="PKJ65_07250"
     CDS             931..>1557
                     /locus_tag="PKJ65_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ41667.1"
                     /translation="MKIVVVGIGGYGEVVLDGLFENPCNLDYSIAGYVAPRPKDNDYS
                     KRLHEMGAQRYATLEEFFEHKHADLAVISSPIEFHMPQTILCLQHNTSVMCEKPVAGT
                     IQDAYAMKKAEEASQAFVGIGYQWSYSDAIVNLKKDILAGMYGKPIRLKNIVLWPRTQ
                     RYFKRNSWAGKIKNDSGYWILDSVANNATAHYIHNMFFVLGDGLKSSAW"
BASE COUNT          481 a          282 c          397 g          397 t
ORIGIN      
        1 tcagatacat aaccaaatgg taaaccatga aaaagcgcaa gtgaccgcaa tttccatgat
       61 agacagagag aagttaccta aggaacttct aaccggggat gttgtgttct atgagaattt
      121 tgacagtatg cttaaagcaa agaatgttga tgttatttca atatgtgctc ccaggagaat
      181 agaccagaag gatttagctg taaaagccct gcttgcgggt aagcatgttt acagtgaaaa
      241 accttgtgct tataccgaaa aggatttgga tgaaatactt gaggtatcaa aaaagaccgg
      301 tatgcttttc catgaaatgg ccggatcggc atttgaggag cctttcattt cggttacgga
      361 gttaattaaa tcgggacgaa taggtgaggt tgttcaggtg tttgcacaga aatcctatcc
      421 ttttaaaaca tcggcggggc gaccccagga tgacataacc gacggcggcc ttatacgctg
      481 gatagccgtt catgccgcaa gattcgtgga acatacaaca ggtttgaaga ttaaagacat
      541 atatgcaatg gaaacgcggc acggaaatcc cgtaaaagac ggcaatctca agatggcggc
      601 aagtattatg ttttctttag ataacaatgc ggtagggtgc attatagcca attatcttaa
      661 tatgaacaat tttccgacat ggggcaacga gcatttaaga gtatggggaa ctgacggttt
      721 tgtggagata accgacggat tgaccgccac cagactggtt actcccgatt atgacggacc
      781 ggtagagatt aagtatgcaa cggcggacta ttttgacttt ttcatagacg agattttata
      841 taacaaacct atgcctttga cgctggaaga ggaacttcat ccgttaagag cggtgataag
      901 agcacaggaa aaagcggtgg aggtgcagta atgaaaattg tagtggtagg tataggcggt
      961 tacggtgaag tggtgcttga cgggttgttc gaaaatccct gcaatctgga ttactctata
     1021 gcgggatatg tcgctcccag acccaaagac aatgattatt ccaaaaggtt gcatgagatg
     1081 ggagcacaaa ggtatgcaac gctggaagag ttctttgagc ataaacatgc ggaccttgcg
     1141 gttatatcca gtcccataga gtttcacatg cctcagacaa ttttatgcct gcagcataac
     1201 acctccgtta tgtgcgaaaa gccggtagcg ggaacaatac aggatgcata tgccatgaag
     1261 aaggcggaag aagcatcgca ggcatttgtg ggaataggat atcagtggtc ctattccgat
     1321 gccattgtaa atctaaagaa agatattctt gcaggcatgt acggaaaacc tattcgtttg
     1381 aagaatatag tgctttggcc cagaactcaa agatatttta agcgaaacag ctgggcggga
     1441 aagataaaga atgattcggg atattggata ttggattcgg tggccaacaa tgccacggca
     1501 cactatatcc acaacatgtt ctttgtttta ggtgacggtt tgaaaagcag tgcatgg
//
LOCUS       DAOPWC010000377         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_90979_length_1552_cov_2.802271, whole genome shotgun sequence.
ACCESSION   DAOPWC010000377 DAOPWC010000000
VERSION     DAOPWC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90979_length_1552_cov_2.802271"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1552)
                     /gene="dnaE"
                     /locus_tag="PKJ65_07255"
     CDS             complement(<1..>1552)
                     /gene="dnaE"
                     /locus_tag="PKJ65_07255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ41668.1"
                     /translation="PEAIIDKDFERAKSTALEYLDIFGEGNFYLELQYNKIPEQELVN
                     KYLIKLSNELNIPLVATNDVHYLEKTDAKAHDILLCIQTAKTVLDENRMRFPSDEFYF
                     KSQEEMMREFSNVPEAITNTQKIADMCDVNFNLNEMHLPKFPLPEGIDHYEYMKDMVF
                     EGLKRRYKNKLTEKHIERAKYELKVINDMKYVDYFLITADFINYAKKNDIPVGPGRGS
                     AAGSIVAYAMGITNVEPIRYNLLFERFLNPARITLPDIDVDFCYERRSEVIEYVKNKY
                     GQDKVCQIITFGTMAARGAIKDVGRALNLPYALCDKISKMIPNELKITIDRALEVNPE
                     LLAEYQTDPSVKELIDMAKKLEGISRHSGIHAAGVVITDNPVKEYIPIQLSDDDSLVT
                     QFPMDNLEELGLLKVDFLGLRTLTVIKDCIDLVRKNHGIDIDFDDMDYDDKNVFEYIS
                     SGNTSGIFQLESAGMTDFMKRFKPQSIEDITLGISIYRPGPMKFIDMCLCNKNSPGGT
                     VYEHELMKP"
BASE COUNT          409 a          340 c          266 g          537 t
ORIGIN      
        1 gggtttcatc agttcatgtt cataaacggt acctccggga ctgttcttgt tacaaagaca
       61 catatctata aatttcatcg gtcccggtct gtatatggaa atacccaatg ttatgtcttc
      121 aatggattga ggcttgaaac gtttcataaa atcggtcatt cccgcacttt ccaactggaa
      181 tatacccgat gtgttaccgg aagatatata ttcaaaaaca tttttatcat cgtaatccat
      241 gtcatcgaag tcaatatcta ttccgtgatt ttttctcacc aaatctatac aatcttttat
      301 aacggtcagt gttcttaatc ctaagaagtc aacttttaac agtcccaact cctccagatt
      361 atccatagga aactgtgtta ctaaggaatc atcgtcggat aactggatgg gaatgtattc
      421 tttaacggga ttatcggtta ttaccactcc cgcagcatgt atcccggagt gtctggatat
      481 gccttccagt tttttagcca tatctatcag ttcttttacc gaagggtccg tctggtattc
      541 cgccaacagt tcgggattga cttccaaagc tctgtcaatg gtaattttca gttcattggg
      601 tatcatcttg cttatcttat cgcataatgc ataaggcaga ttgagtgctc tgccgacatc
      661 cttaatggct cctcttgccg ccatggtacc gaaagttatt atctgacaca ccttatcctg
      721 tccgtatttg tttttaacat attcgattac ttccgatctt ctctcataac agaagtctac
      781 gtcaatatcg ggcaaagtta ttcttgcggg attaagaaat cgttcaaaaa gcagattata
      841 tctgataggc tccacattgg ttatacccat agcatatgcc actatacttc ctgcggcact
      901 tcctctgccc ggtccgaccg gtatatcgtt tttctttgcg taattgatga aatccgccgt
      961 tattaagaag tagtcgacat atttcatatc gtttataact ttaagctcat atttggctct
     1021 ttcaatgtgt ttttctgtca atttattctt gtatcttctc ttaagtccct caaataccat
     1081 atccttcatg tattcgtaat ggtcaatacc ctcaggtaaa ggaaacttgg gaagatgcat
     1141 ttcattaaga ttaaagttta catcgcacat atccgctatt ttctgtgtat tggttatagc
     1201 ctcgggaaca ttggaaaact ccctcatcat ttcttcctgt gatttgaaat agaactcgtc
     1261 cgacggaaat ctcatcctgt tctcatccaa aacggtcttt gcagtctgga tacacagcag
     1321 aatatcatgt gcttttgcat cggttttttc caggtaatgt acgtcatttg ttgcaaccaa
     1381 aggtatattc aactcattgg agagttttat gagatattta tttaccagtt cctgctccgg
     1441 tatcttgtta tattgcaact ccagataaaa attgccttca ccgaatatat ccagatactc
     1501 taaagctgtt gatttcgctc tttcaaagtc cttatcaata atagcctcgg gt
//
LOCUS       DAOPWC010000378         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_91391_length_1547_cov_3.367292, whole genome shotgun sequence.
ACCESSION   DAOPWC010000378 DAOPWC010000000
VERSION     DAOPWC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91391_length_1547_cov_3.367292"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKJ65_07260"
     CDS             <1..1002
                     /locus_tag="PKJ65_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41669.1"
                     /translation="GTNFRLRVNIPTTVKDGEQFVFSFAFSRTYSSQTIYVSTPSKIY
                     TFQSEVVSSGIYKRYSFVFTKSAEDSYVEIYTSAATANWTLNSKEYQIELGNKATDWT
                     PAPEDVQEDYATKLGYSSYSDLVTKATAGSTIIKGGYLNTDLIEARSIVAGKIATGTI
                     TAKEIDVSSLTVKFLKTVGDANGYKVQIENSKNSDDSGAESYVRLVNSDGTRKAELVF
                     DDNNVYGGSGGLPVLRITDDGCYSRVFAEGLEAVSTTDGRFEYNMISQFTPFGMVFAT
                     GTHSSAAVKGGFTWNKDDEKVLIYVNTLPRGGSGTVSEGFLYRSPDGLNNDVIRYKS"
     gene            999..>1547
                     /locus_tag="PKJ65_07265"
     CDS             999..>1547
                     /locus_tag="PKJ65_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41670.1"
                     /translation="MTREEYYNLLCEKNSFYYWDKESDVIGKMFELRPSFITKHRKVV
                     AGADELLNAYQATTYKDEETRKRAENILAFANEEKRKSQEILKTEEASIQQYLDMEKP
                     VLVVSDGEYMEIVDISEYEEIQGMSDEEALLWYRQMRISKAMESFIKSDFYGDDFGLM
                     CLRMRADLDTLYEWVKSIQDMIP"
BASE COUNT          527 a          230 c          366 g          424 t
ORIGIN      
        1 ggaacaaatt ttaggttgag ggtaaatatt ccaacaactg taaaggatgg agaacagttt
       61 gttttttctt ttgcgtttag taggacatat agttctcaga caatatatgt atcaacacca
      121 tcaaagatat atacattcca aagcgaagta gtttcttctg gcatatacaa gcgatacagt
      181 tttgttttta caaaaagtgc agaagatagt tatgtagaaa tatatacatc tgcagctaca
      241 gcaaactgga cattaaattc taaggaatac cagattgaac tgggcaacaa agccaccgat
      301 tggacacctg ccccggaaga tgtgcaagag gattacgcca ccaagttagg ttactccagc
      361 tattcagacc ttgtaactaa ggccacagca ggctctacaa taataaaagg aggttatctt
      421 aatacagacc ttattgaagc aaggtctatt gtagcaggga aaatagccac aggcactatc
      481 actgcaaaag agattgacgt ttccagtttg accgtaaaat ttttaaaaac agttggagat
      541 gccaatggat ataaagtaca gatagaaaat tcaaagaatt cagacgattc cggtgcggaa
      601 tcttatgtta gactcgtaaa ttccgatggt acaagaaaag cggaattggt atttgacgac
      661 aataatgtgt atgggggttc ggggggactt ccagtattac gaataacgga cgacggttgt
      721 tattcaagag ttttcgccga gggtcttgaa gcagtaagta cgacagacgg aaggttcgaa
      781 tataatatga tttcacagtt cacaccattc ggaatggttt tcgcaacagg tacacactca
      841 agtgccgcag taaaaggcgg atttacttgg aataaggatg acgaaaaggt gttgatatac
      901 gtaaatacct taccaagagg tggttctggg actgtcagtg aggggttctt gtacaggtct
      961 cctgatggat taaataatga cgtaataagg tataaatcat gacaagagag gagtattata
     1021 atcttttatg cgaaaaaaat tccttttact attgggacaa ggaatctgat gtaataggaa
     1081 aaatgtttga gttaagacct tcttttatca caaaacacag aaaagtggtc gcaggtgcgg
     1141 atgaattgtt aaatgcgtat caggctacca cgtataaaga tgaggaaaca aggaaaagag
     1201 ccgaaaatat tttggctttt gcaaatgagg aaaaaagaaa gtcgcaggaa atattaaaaa
     1261 cagaggaagc ctctatacag caatacttgg atatggaaaa accagtactt gttgtttctg
     1321 acggggagta catggagatt gtggatattt ccgagtatga ggagattcag ggaatgtctg
     1381 atgaggaggc tttgttgtgg tatagacaaa tgagaatatc aaaagcaatg gagtctttta
     1441 taaaatcgga tttttatggg gatgactttg gtttgatgtg tttgcgtatg agggcagatc
     1501 tggatacact ttatgaatgg gtgaaaagta ttcaagatat gattcct
//
LOCUS       DAOPWC010000379         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_91577_length_1545_cov_2.351678, whole genome shotgun sequence.
ACCESSION   DAOPWC010000379 DAOPWC010000000
VERSION     DAOPWC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91577_length_1545_cov_2.351678"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKJ65_07270"
     CDS             <1..223
                     /locus_tag="PKJ65_07270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ41671.1"
                     /translation="EFLIICPDTDYVGITDIAKRIEAFVSGYNFGCCQGVALSCGVAT
                     HNSGETVDSLLQRADTSMYQTKQSRRKNI"
     gene            complement(278..1510)
                     /locus_tag="PKJ65_07275"
     CDS             complement(278..1510)
                     /locus_tag="PKJ65_07275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ41672.1"
                     /translation="MFFDIVEKTDKYLEYMDYFILGPSVVRCIHSDDLLMNFDLFNAS
                     DSNAGSVLLRSKDNGNTWIEQGLIEKSYVYDFNNSRVKSGYGALYSDTDAKVILYVAN
                     DTYWEENKIASLWRMRTLYYRLSFDDGYTWTDKKYIVQKGDCYNTKHFMKSVEFGVNM
                     AATVSPLIVHAKDNSLLVPVQVQMLDKDGTLFNPTGMGYMKAGALKAVWNSEKLEYEW
                     DLGEYVTVEPDKSVRGLYEPTFGIIDGKENEILMVMRNSNYTQSDKITGAKFYSISED
                     NGYTWSKPEMLLYDDGSIMYSSSSIPKLLNHSNGNLYFIGIINRENPKGNLPRYPLCI
                     AKVDKETKRVIKASVTVIDTKREHHNLSVKTSNVVDYSNHGVYEDKETKNIVVLAPYR
                     ENLSQYRCYLNRYVVKVK"
BASE COUNT          433 a          351 c          267 g          494 t
ORIGIN      
        1 ggagttcctt ataatctgtc ccgacaccga ctatgtaggt attacggata tcgcaaaaag
       61 aatagaagca ttcgtttccg gatacaactt cggctgttgc caaggagtgg ccttaagctg
      121 cggagttgct actcacaaca gcggtgaaac agtggattct ctgctccaaa gagcggatac
      181 aagcatgtat cagactaagc aatcccgcag aaaaaacata taactttttt attgaaaaat
      241 aataaaatcg ataagtaagc gatgcaaaac gtaaggctta cttaactttt actacatatc
      301 tgttcagata acagcgatat tgcgaaagat tctccctgta aggagccaat acaactatgt
      361 tttttgtttc tttatcctca taaaccccgt gattggaata gtccactaca ttggaagttt
      421 ttacacttaa gttgtgatgc tctcttttgg tatctataac tgtgactgat gcttttatta
      481 ctctcttggt ttccttatcg acttttgcta tgcataaagg atatctcggc aggttccctt
      541 tgggattttc tcggtttatt atacctatga aatagagatt accgttactg tgattgagca
      601 atttgggtat gcttgaagaa gagtacatta tactgccgtc atcatacaga agcatttccg
      661 gtttagacca ggtatagccg ttatcttctg atatactgta gaattttgca ccggttattt
      721 tatccgactg cgtataattg ctgttacgca taaccattaa tatttcattt tcttttccgt
      781 ctattattcc gaacgtaggc tcatacagtc ccctgaccga tttgtccggc tccactgtca
      841 catactcccc taaatcccat tcatattcaa gtttctcact gttccatacc gcttttaaag
      901 cccccgcttt catatatccc atacccgtgg gattaaaaag agtaccgtct ttatccagca
      961 tctgtacctg taccggcacc agaaggctgt tatcttttgc atgtactatg aggggagaaa
     1021 ccgtagctgc catattaact ccgaattcca ccgacttcat gaaatgtttt gtgttatagc
     1081 aatcgccttt ttgcacaatg tatttcttat ccgtccaggt atatccgtca tcaaatgaca
     1141 gtctgtaata caaggtcctc attctccaca gggaagctat cttattttcc tcccagtatg
     1201 tatcatttgc gacatataaa attacctttg catcggtatc agaatacagc gccccatagc
     1261 cgcttttaac ccggctgttg ttaaaatcat atacatagga tttctctata agcccctgtt
     1321 ctatccaggt attgccgtta tcttttgaac gtaaaagcac actgccggca ttgctgtccg
     1381 atgcattaaa caaatcgaaa ttcatcagta aatcgtcgga atggatgcaa cgtacaaccg
     1441 acggtcccaa tatgaaatag tccatatatt ccagatactt atcggtcttt tcaacaatgt
     1501 caaaaaacat aataacccct cagtcccgct aacattatat catct
//
LOCUS       DAOPWC010000380         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_91894_length_1541_cov_4.142665, whole genome shotgun sequence.
ACCESSION   DAOPWC010000380 DAOPWC010000000
VERSION     DAOPWC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91894_length_1541_cov_4.142665"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKJ65_07280"
     CDS             <1..886
                     /locus_tag="PKJ65_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ41673.1"
                     /translation="ISPQYEVRVLEENEKQITYTTNYGVTLRKFKEEDSTPEFLDYKV
                     DNSKIWFEETKKRMKEGTNRINWERYGRVYPEWVADGHWIQAGFWFGFDVTHSWMCGT
                     ETILIAMLEQPEWVTDMFDTYLNSCIRHFDILWDKGYRFDSIFWYDDMGYKNKTFFSL
                     DTYRELVKPFHKKAVDWAHNKGIYAHLHSCGCVMNFVEDLVEIGVDALNPLEIKAGMD
                     PILLKKKYGDRLVLHGGINAVLWDKVDEVTEAIIQNVPILKENGGYIFSSDHSIPNTV
                     SLENFKSIIKTVKEAGKY"
     gene            complement(997..1533)
                     /locus_tag="PKJ65_07285"
     CDS             complement(997..1533)
                     /locus_tag="PKJ65_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNZ41674.1"
                     /translation="MKNLKCTKTEANLKEAFAGESMARNKYSYFASKAKKDGYVQIAN
                     IFEETANNEKEHAKIWFKLLEGIGDTPVNLEHAAEGENYEWTDMYPRFAKDAKAEGFK
                     EIAVLFEQVAKIEKDHEERYRKLLANINDGIVFSRDSETIWECTNCGHVVIGKKAPQM
                     CPVCDHPRDYFEIKVVNY"
BASE COUNT          484 a          305 c          298 g          454 t
ORIGIN      
        1 tatatccccg caatatgaag tcagggtact ggaagaaaac gaaaagcaga ttacctatac
       61 caccaattac ggagttacat taagaaagtt caaggaagaa gactctaccc cggaatttct
      121 ggattataaa gtggataatt caaaaatctg gtttgaagaa acaaaaaagc gaatgaaaga
      181 aggcacaaac agaataaatt gggagcgtta cgggagagta taccctgaat gggtcgcaga
      241 cggtcattgg atacaggcgg gattctggtt cggatttgat gtaacccact cctggatgtg
      301 cggtaccgaa acaatactga tagcaatgct ggagcaaccg gagtgggtta cggacatgtt
      361 tgatacctac ctgaattcct gcatcaggca ttttgacata ctgtgggata agggctacag
      421 attcgattcc atattctggt atgacgatat gggatataaa aacaagacat ttttctcttt
      481 ggacacctac agagaattag taaaaccttt ccataaaaaa gccgtggatt gggcccataa
      541 taaaggcata tatgcacatt tacattcttg cggttgtgtt atgaactttg tggaagatct
      601 ggtggagata ggagtggatg cactcaatcc cctggaaata aaagccggaa tggatcccat
      661 cttattaaaa aagaaatacg gagacagatt ggttctccac ggcgggataa atgccgtact
      721 ctgggataag gttgatgaag taaccgaggc aattatacaa aatgttccca ttttaaagga
      781 aaacggaggc tatatattct cctctgacca ttccataccc aacacggtca gtctggaaaa
      841 ctttaagagc ataataaaga ccgttaaaga ggcgggaaaa tattaaatat atactccgat
      901 atcttttgca gcataaataa aataacggat taaacaaaaa aagacagcaa cggctgtcct
      961 ttttgttgtt tcaatattat actcggaaat taacggttaa taattaacaa ccttaatctc
     1021 aaagtaatct ctcggatgat cacataccgg acacatctga ggtgcttttt tacctataac
     1081 aacatgcccg cagttggtgc attcccagat tgtttcggaa tctcttgaga aaacaatacc
     1141 gtcattgata ttggcaagca gttttctgta tctctcttca tgatcttttt ctatctttgc
     1201 tacctgctca aaaagaactg caatctcctt aaatccttca gcttttgcat cttttgcaaa
     1261 tcttggatac atatcggtcc attcgtaatt ctcgccttct gccgcatgct caagattaac
     1321 cggtgtatcg cctatacctt ccagcagttt aaaccaaatt tttgcgtgtt ctttctcatt
     1381 attagccgtt tcctcaaaaa tattggctat ctgcacatat ccgtcttttt ttgctttgga
     1441 agcaaaataa gaatacttat ttcttgccat ggattcccct gcgaaagcct cctttaaatt
     1501 agcttccgtc tttgtacatt ttaaattttt catatattac t
//
LOCUS       DAOPWC010000381         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_91908_length_1541_cov_2.936743, whole genome shotgun sequence.
ACCESSION   DAOPWC010000381 DAOPWC010000000
VERSION     DAOPWC010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91908_length_1541_cov_2.936743"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKJ65_07290"
     CDS             <1..885
                     /locus_tag="PKJ65_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41675.1"
                     /translation="VLQGISGTGKTSLPYCFGKFIKNDVTMASVQPSWRDRTELFGYF
                     NEFTKKFNETEVLRRIYEASYNDDINMIVLDEMNIARIEYYFAEMLSVLEMPDPEQWQ
                     LELVPSSWTSDPRNLVGGKLRIPENIWYLGTANNDDSTFAVSDKVYDRAFTINFESKG
                     QPFDAPVTEARPISFSYLEELYSDAWERYPVSKENLDKIAKLDLYIIQKFRIAFGNRI
                     MKQLHEFVPVYVACGGKEIDGIDYVLYTKVLRKFESLNLSLIRDDLKEFVNYLNRSFG
                     RNNMNECKKYLELLQRMY"
     gene            910..>1541
                     /locus_tag="PKJ65_07295"
     CDS             910..>1541
                     /locus_tag="PKJ65_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41676.1"
                     /translation="MAKRRKKLILDDEQRDSIYEDTKDFISYIYEDYDFYQSLVRLLD
                     RGKNQAVLYRKVLQKNIDELWVKRVEDALPYLDTALRAVRKDIKEVEEIKPIALSRNI
                     TVKSLQHLATHTNFIDRIDEKGNVIPNRLLNVYKDEEILTYENKFLNTLIYKLALFID
                     VRYEKLKEYGADEVSNVMSMDIETKVVDTELKMSITIETKDGVDEQDKKD"
BASE COUNT          530 a          249 c          341 g          421 t
ORIGIN      
        1 gtacttcagg gtatatccgg taccggtaaa acatcgctac cgtactgttt cggaaaattt
       61 attaagaatg atgtcacaat ggcttcggtt cagccttcct ggagagacag aaccgagttg
      121 ttcggatatt tcaatgagtt taccaagaaa ttcaacgaaa ccgaagtttt aagaagaata
      181 tatgaagcat catataacga tgacatcaat atgattgttc tggacgagat gaatattgca
      241 cgtatagagt actactttgc cgaaatgctt tcggttttgg aaatgcccga tcccgagcag
      301 tggcaactgg agctggtacc cagttcctgg acatccgacc cgagaaatct ggtaggcggt
      361 aaattacgga taccggaaaa tatctggtat ctgggaacgg ctaataatga cgactccact
      421 tttgcggttt ccgataaagt atatgaccgt gcatttacaa ttaactttga atcaaaggga
      481 caaccgtttg atgcaccggt tacggaagca cgccctataa gtttctctta tcttgaggag
      541 ctttactccg atgcatggga gagatatccc gtttccaagg aaaaccttga taagattgcc
      601 aaactggatt tatacattat tcagaagttc agaatagctt tcggtaaccg tattatgaaa
      661 cagctgcacg agtttgtacc ggtatatgtt gcatgcggcg gcaaagaaat tgacggtatt
      721 gactatgttc tgtataccaa agttttaagg aaattcgaaa gtctcaacct gtcattgata
      781 agagatgact tgaaggagtt tgttaattat ctgaacagaa gtttcggaag aaacaatatg
      841 aatgagtgta aaaaatatct tgaactgctt cagagaatgt attaatggat tttgcaggag
      901 ggtattatca tggcaaaacg aagaaaaaag ttaatacttg atgatgagca gagagattca
      961 atatacgaag atacaaaaga ttttatttca tacatatatg aagattatga cttctatcaa
     1021 tcgcttgtgc gtttactgga cagaggtaaa aaccaggcgg ttctttacag aaaggtgctc
     1081 cagaagaata ttgatgaatt atgggtaaaa agagtggaag atgctttacc ttatctggat
     1141 accgcattaa gggctgtgag gaaggatata aaagaggtgg aagagataaa acccatagca
     1201 ttatccagaa acataacagt aaaatcttta cagcatcttg cgacacatac caattttata
     1261 gacagaatag acgaaaaggg taatgttatt ccgaacaggc ttctgaatgt atataaagat
     1321 gaggaaatac ttacatacga gaataagttt ttaaacaccc ttatatacaa attggctctt
     1381 tttatagatg tcagatatga aaaactgaag gaatacggtg ccgatgaagt atccaatgta
     1441 atgtccatgg atattgaaac caaggttgtg gataccgaac tcaagatgag tattaccata
     1501 gagacaaaag acggagtgga cgaacaggat aaaaaggata a
//
LOCUS       DAOPWC010000382         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_92036_length_1540_cov_2.342088, whole genome shotgun sequence.
ACCESSION   DAOPWC010000382 DAOPWC010000000
VERSION     DAOPWC010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92036_length_1540_cov_2.342088"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PKJ65_07300"
     CDS             complement(<1..1103)
                     /locus_tag="PKJ65_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ41677.1"
                     /translation="MSKSKETERILKLLEEKKITSEEAKKLLDALSAEYDKESSDKDN
                     AKRDNYDTPDNIFDKYEGIGEDIKDAIAEKMRGKYSDLGEDIRKSVLAGISNIKNNIL
                     ESLSSDIDNSINSDCSSAVKREYELVSGATVKIKGANSCVNIKRGTGDKVILSVSVSP
                     FDYDVINELLEIEYSKDCLDISVDDSCKAGVTITLLLPDMQYADTEINTTNGSVNITD
                     VKCTAIMLASSNGQLTVKRCTGSTLKVETSNGQVRTEGNNFDNSVIVKSTNGSLISKT
                     DISKMIHMETTNGSVRLYSSYARSFKAETTNGSLLFDRLHPYQAENEFLIRGETTNGS
                     TTVNINYNFGAVFECSAGKHGSIRLGSNLEAQSA"
     gene            complement(1116..1475)
                     /locus_tag="PKJ65_07305"
     CDS             complement(1116..1475)
                     /locus_tag="PKJ65_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNZ41678.1"
                     /translation="MKNYVPSQCPICEGELIITDMHCKSCKTHISGEFYTTKLAKLDP
                     GQLEFVETFIKCRGSIKEMEKELKISYPTVKSRLELIAETMGYKDTEQKDDTRMKVLD
                     SIEKGEMTVEEALKILK"
BASE COUNT          384 a          360 c          251 g          545 t
ORIGIN      
        1 gccgactgtg cttctaaatt acttcccaga cgtatagaac cgtgttttcc ggctgagcat
       61 tcaaacaccg ctccgaaatt gtagttaata ttaactgttg tcgaaccgtt tgttgtttcc
      121 ccgcgaatca gaaactcatt ctccgcctga taaggatgca gtctgtcgaa tagcagcgaa
      181 ccgttggttg tttccgcctt aaaactcctt gcataactgc tgtacaaccg gaccgaaccg
      241 ttggttgttt ccatgtgtat cattttagag atatcggtct tggatattaa agatccgttt
      301 gtggacttta caataactga gttatcaaaa ttgtttcctt cggttctcac ctgtccgtta
      361 cttgtctcaa ctttcaaagt actccccgta catcttttta cagtcagttg accgttggaa
      421 cttgccagca ttattgctgt acatttaaca tccgttatgt ttacggaccc gttagttgta
      481 ttgatttcag tatcggcata ctgcatatcg ggaagcaaaa gtgtaatcgt aacccccgct
      541 ttacaactgt catctacgga aatgtccaga caatcttttg aatattctat ctccaatagc
      601 tcatttatga cgtcataatc aaacggtgac accgatacgg aaagaattac cttatcacct
      661 gtacctcgtt ttatattaac acaactgttt gcacccttaa tttttacagt tgcaccgcta
      721 accagctcgt actccctttt tactgcggaa gaacagtcgg aattgatgct gttgtcaata
      781 tcagaagata gcgattccag aatattattt ttaatgttgg aaatccccgc taacaccgat
      841 ttcctaatat cctctcccag atcggaatat tttccgcgca tcttttccgc tattgcgtct
      901 tttatgtctt ctccaatacc ctcatattta tcgaatatat tatcaggggt atcataatta
      961 tcccgttttg cattatcttt atcggaactc tccttgtcat actctgccga taaagcatcc
     1021 aacagctttt ttgcttcttc cgaagtaatc ttcttttcct ccagaagttt caatattctt
     1081 tcagtctctt tggatttact cataacattc ccccgttatt taagtatttt caatgcttct
     1141 tcaacagtca tctcaccttt ctcaatacta tcgagtactt tcattctggt gtcgtctttc
     1201 tgctccgtat ccttatatcc catcgtttcg gcgataagct ccagacgtga tttgacggtg
     1261 ggataagata ttttcaattc cttttccatt tcttttatac tgcctctgca ttttataaaa
     1321 gtttcaacaa attccaactg gccgggatcc agtttagcaa gtttagtggt atagaactcg
     1381 ccgcttatat gtgtcttaca acttttacaa tgcatatcgg tgattatcag ttcgccctcg
     1441 catatcgggc attgtgacgg tacatagttt ttcatgttat tctctccata atatagtata
     1501 ggcacttatt ttctcaagat tatatgccaa tgttacaaag
//
LOCUS       DAOPWC010000383         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_92840_length_1531_cov_3.171409, whole genome shotgun sequence.
ACCESSION   DAOPWC010000383 DAOPWC010000000
VERSION     DAOPWC010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92840_length_1531_cov_3.171409"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1170
                     /locus_tag="PKJ65_07310"
     CDS             265..1170
                     /locus_tag="PKJ65_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ41679.1"
                     /translation="MKKIDIHVHSRMYLTEEERNAIYQPIKDKLTYPAPEDIAILYKR
                     LGIEKGVLLVTVAPEGAHYTFTNLEAMRIVRRYPDLFYWFCGIDPRCWTNSPDTDLSH
                     FINHYKKRGAKGIGEVTAKLPFSDERMQNLFRHAEMCDMPVLIHIGTDDSSYGIYDVK
                     GLKQLEETLIRFPKLTIIGHSQPFWAEIGADSEERDRFGYPSGPVKKAGRLVELMRKY
                     DNLHADLSANSGYDAMTRDPDFGYAFLEEFQDRIYYGTDICSKREEKSFKLAQFLEQG
                     VEKNKLSDRAYRKICRTNALRLLER"
     gene            1181..>1531
                     /locus_tag="PKJ65_07315"
     CDS             1181..>1531
                     /locus_tag="PKJ65_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ41680.1"
                     /translation="MKRIDMHIHSVMYSDDWEKQNDIPRVYRKETFATPDEIKPYYDK
                     LSIEKGVLLPVVSIEGQHRLINNEETYRIVRAYPDMFDWYCNIDPRWHYNSEETDLGF
                     FIEHYKKLGAKGIGE"
BASE COUNT          546 a          257 c          333 g          395 t
ORIGIN      
        1 atccattccg ctatcggaac aggtttcttc tctttcataa cccagtcctt tcaatcttaa
       61 tacatgaatt atatcatccg ttactgaaat ctcaatgaag atgaataata aaaaagaata
      121 aaaaatgtaa aatgcaaagt ttttaatatt aaaaatttaa agataattga tgacattaat
      181 gtgttagaat atatgaggtc agcattcatt ataacggttt tattaaaaat ataaccgctg
      241 tatacgtttt aaaagaggga atatatgaaa aaaattgata tccatgttca cagcaggatg
      301 tatctgacgg aagaagaaag aaacgctata tatcaaccga taaaagacaa gcttacctat
      361 cctgctcccg aagatatcgc gatattatat aaaaggctcg gtattgaaaa gggagtactt
      421 ttggtaaccg tagctcccga aggtgcacat tatactttca ccaacttgga agcaatgaga
      481 atagtaagaa gatatcccga cctgttttac tggttttgcg gaatagaccc cagatgctgg
      541 acaaacagtc ccgataccga tctttcacat tttataaacc attataaaaa gcgcggtgca
      601 aaaggtatag gtgaagtgac cgcaaagttg cctttttcag acgagcgtat gcaaaacctg
      661 ttcagacatg ccgagatgtg cgatatgccc gttttaattc acataggtac ggatgacagc
      721 tcttacggta tttatgatgt aaaaggatta aagcagctgg aagagacttt aataagattt
      781 cccaaactta ctataatagg gcattctcaa cctttctggg cggaaatagg tgccgattcg
      841 gaggagcggg acagattcgg atatccttcg ggacctgtta aaaaagccgg aagactggtg
      901 gaacttatga gaaaatacga taatcttcat gccgacctgt cggcaaacag cggatatgat
      961 gcgatgacaa gggaccccga tttcggatat gcattcttgg aagaatttca ggacagaata
     1021 tactacggta ccgatatctg ttcaaaaaga gaggagaaat cattcaaatt ggcacagttt
     1081 ttggaacagg gcgtggaaaa gaacaaattg agcgataggg catacaggaa aatatgcaga
     1141 acaaatgctt taaggctttt ggaaaggtaa aggggaaaac atgaaaagaa ttgacatgca
     1201 tattcactcc gtaatgtatt cagatgattg ggaaaaacaa aatgacatac cgagagtata
     1261 caggaaggaa acatttgcaa cacccgacga gataaagcca tattacgaca aattgagtat
     1321 tgaaaaagga gttcttttac cggtagtgtc aattgaagga cagcacagac tgataaataa
     1381 tgaggaaact tacaggatag tgcgcgcata tccggatatg tttgactggt attgtaatat
     1441 agatcccaga tggcattaca actcggaaga aaccgatttg gggttcttta tcgagcacta
     1501 taagaagctg ggagccaaag gaataggaga a
//
LOCUS       DAOPWC010000384         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_93039_length_1529_cov_3.254410, whole genome shotgun sequence.
ACCESSION   DAOPWC010000384 DAOPWC010000000
VERSION     DAOPWC010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93039_length_1529_cov_3.254410"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..435)
                     /locus_tag="PKJ65_07320"
     CDS             complement(34..435)
                     /locus_tag="PKJ65_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNZ41681.1"
                     /translation="MKKFIARVNGKEYVVELEEVTGTPAVSAPVVTVAAPVAAPVVKE
                     EVKQEVPAPKKTVSSGAAGSVRIEAPMPGTILSVKVSVGQAVKKGQAVAVLEAMKMEN
                     DIVAAQDGVIVSVNVSNGESVETGQLLVSMD"
     gene            complement(454..>1529)
                     /locus_tag="PKJ65_07325"
     CDS             complement(454..>1529)
                     /locus_tag="PKJ65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ41682.1"
                     /translation="SGVIPQISVVLGNCAGIFSLFTAMSDFVVMADKISYMYINGPEA
                     ILGISGVKTTDEEIGSAATHATSSGNAHIHCENEDDCINAVKLLLSYLPDNNLTEALK
                     LEVSDAPDRTAENLYETVADSYDMREVITSVTDTSTFMEIQSEFAASMITGFARMNGQ
                     SVGIVANNPAVNEGRIDMHAADKAARFVRLLDCFNIPIITFTHTSGYVADALQEKEGL
                     IRHAAKMVYAFTEADVPKINIITGKAIGGAYIAMNSKATGCDIVFAYPTASVAVMDSE
                     GAANIIYNEQIAKSDNPVEFRKAKIKEYHERFASPYEAAKRGYVDDVIDPAFTRAYVI
                     TALEMLKSKRENRPNRKHGNMPL"
BASE COUNT          371 a          386 c          284 g          488 t
ORIGIN      
        1 ctcagtgatt tgtactctca tcttgaaccc tttctaatcc atggacacta aaagctgtcc
       61 cgtttccaca ctctctccgt tggatacgtt aaccgatacg ataacaccgt cctgtgcggc
      121 aactatatcg ttctccattt tcattgcctc cagtacggca acagcctgac ctttcttgac
      181 agcctgtcct accgatacct ttacacttaa aatggtaccg ggcatgggag cctctattct
      241 gacagagcct gccgcgccgg aagatacggt ctttttggga gcaggaactt cctgctttac
      301 ttcttcctta acaacaggtg cagcaacagg cgccgctacg gtaacaacag gtgccgaaac
      361 ggcaggtgtt cccgtaactt cttccaattc cactacatat tcttttccgt taactcttgc
      421 tatgaacttt ttcatatata tttcctccgt ttattaaagc ggcatattgc catgttttct
      481 gttaggacga ttttctcttt ttgatttgag catttccaat gcagttatta cataagcacg
      541 cgtaaatgcc ggatctatga catcatccac atatcctctt tttgcggctt cgtaaggtga
      601 tgcgaatcgc tcatgatatt ctttaatctt tgcttttctg aactcaacgg gattgtcgct
      661 ttttgctatc tgctcgttgt aaattatatt ggcggctcct tcgctgtcca ttaccgccac
      721 acttgcggtg ggataagcaa atacaatatc gcaacctgta gccttgctgt tcatcgctat
      781 gtaagcgccg cctattgcct tacccgtgat tatatttatc ttaggcacat cggcttccgt
      841 gaatgcatat accatttttg ccgcatgcct tatcagtcct tctttttcct gcaatgcatc
      901 cgcaacatag cccgatgtat gtgtaaaagt aattatcgga atattaaagc aatctaaaag
      961 tcttacgaat cttgcagctt tatcggcggc atgcatatcg atgcgacctt catttaccgc
     1021 aggattgttt gccacaatac ctaccgattg accgttcatt cttgcaaaac ccgttatcat
     1081 ggaagcagcg aactccgatt gtatttccat aaaggttgat gtatctgtta ccgaagtgat
     1141 aacctccctc atatcatatg aatctgctac cgtctcatac agattttccg cagttctgtc
     1201 gggagcatcc gatacttcaa gttttaaagc ttcggtaaga ttgttatcgg gaagatagga
     1261 taaaagcagt ttaaccgcat ttatacagtc atcttcattc tcgcaatgta tgtgcgcatt
     1321 gccgcttgat gttgcatgtg tagcggcact tcctatctct tcgtcggttg tttttactcc
     1381 ggaaatacct aagatagctt ccggtccgtt gatgtacatg tagcttatct tatccgccat
     1441 aaccacaaaa tcactcatgg ccgtaaacaa tgaaaaaata ccagcgcaat tgcccagtac
     1501 cacacttatt tgaggaatca ctccgcttg
//
LOCUS       DAOPWC010000385         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_93166_length_1528_cov_2.782077, whole genome shotgun sequence.
ACCESSION   DAOPWC010000385 DAOPWC010000000
VERSION     DAOPWC010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93166_length_1528_cov_2.782077"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKJ65_07330"
     CDS             complement(<1..1172)
                     /locus_tag="PKJ65_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ41683.1"
                     /translation="MTTQNPSDRKYDRPPQGGRGGGGPRATMRLVEKPKDFKGAMNKL
                     LKYLKPYWFLLLVSVIFSVASTVFNILSPKQMGLITTELYVGMTQKNGLIDFNYIGRI
                     ILILLLLYIGYTLFHVIQGLISNTVSQKIGYEMRKSMADKCNRLPLSYFDTNLHGDIL
                     SRLTNDIDTITMTLNQSLSQITTTVTQILGVFIMMLLISPLMTAVAVLILPLSSVFVA
                     VLVKKSQKFFKAQQENLGNLNGHVEEMFSGHLVLKVFNGEERSVDKFRTFNDKLFESS
                     RKSQFLSGLMFPVTGFIGDIGYVAICVLGGYLVLSNSIQVGDIQAFIRYVRQFNNPLR
                     QTAGIANTLQSTAAAAERVFEFLEEAEEIPDPENAYNISDFKGNVSFKNVFFGYDK"
     gene            complement(1169..1468)
                     /locus_tag="PKJ65_07335"
                     /pseudo
     CDS             complement(1169..1468)
                     /locus_tag="PKJ65_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          450 a          309 c          284 g          485 t
ORIGIN      
        1 ttcttatcat atccgaagaa aacattctta aaggatacat ttcccttaaa gtccgagata
       61 ttgtatgcgt tttccggatc gggaatttct tccgcttctt ccagaaattc aaacaccctt
      121 tcggctgccg ctgctgtact ttgcaaagta tttgctatac cggctgtctg tcgtaacgga
      181 ttgttgaact gtcttacata tcggataaag gcctgaatgt caccgacctg aatgctgttg
      241 gataacacca gatatcctcc taatacacat atggccacat atcctatatc tccgataaaa
      301 ccggtaacgg gaaacatgag tcccgataaa aattgtgatt ttctggaact ttcaaagagt
      361 ttatcgttga atgttctgaa tttatccacg gatcgttctt caccgttaaa aactttaagt
      421 actaaatgac cggagaacat ttcctcaaca tgcccattca gattgccgag gttttcctgc
      481 tgagctttaa agaacttttg agattttttt accaatacgg caacgaaaac cgatgaaagg
      541 ggaagaatga gtacagcaac cgctgtcatc agaggagaga taagcaacat cattataaat
      601 acacccaaaa tttgtgtgac cgtagtagtt atctgagata aactctgatt taaagtcatt
      661 gtaatggtat caatatcatt tgttaatctg ctcagtatat cgccatgtaa gttggtgtca
      721 aagtaactca acggaagtct gttacattta tcggccatgc tttttctcat ctcatatcct
      781 atcttttgtg aaacggtatt ggatatcaaa ccctgtatta catgaaaaag ggtataacct
      841 atataaagga gcagtaatat gagaatgatt cttccgatat agttaaaatc aatcaggccg
      901 ttcttctgtg tcattcctac atacaactcg gtagttataa gacccatctg tttcggtgaa
      961 agaatattaa aaacggtaga tgcaacggaa aatattaccg atacaagcag caaaaaccag
     1021 taaggcttta ggtatttaag cagtttattc attgctccct taaagtcttt gggtttttcc
     1081 accagtctca ttgtggctct cggtcctcct ccgcctcttc cgccttgcgg aggtctgtca
     1141 tattttctgt cggatggatt ttgagttgtc ataccagctc ctcctttgtc aattgcgatg
     1201 aagctatctc ataatagacc ttgcatgttt taagcaaatc gttatgtttt cccataccta
     1261 caacttttcc cgcatccaga acaataatct gatcggcatt cattattgta ctgactcttt
     1321 gtgtaataag aataaccgtg gaattcagtg tcttttcggc aagagcgttt tttaaattgg
     1381 actcggtctg aaaatccagt gaggaaaaac tgtcatcaaa tatatatatc ttaggggaat
     1441 cggctaaagc acgtgctatg gataatcgat tcccctaaga tatatatatt tgatgacagt
     1501 ttttccgtgg aattcagtga ggaaaaac
//
LOCUS       DAOPWC010000386         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_94079_length_1519_cov_2.937158, whole genome shotgun sequence.
ACCESSION   DAOPWC010000386 DAOPWC010000000
VERSION     DAOPWC010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94079_length_1519_cov_2.937158"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKJ65_07340"
     CDS             <1..510
                     /locus_tag="PKJ65_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ41684.1"
                     /translation="FVGVAFVVFNGTFILKLNPLGDLLAFLSALSWAVYSLILKKIIN
                     RYDNILLIRKVMFYGIVTTAPFLLLEGKPFDFAAFSDPKLIISILFLGVVGSGLCYMI
                     WNIPVRTLGIRITNSYIYAIPFVTMILAATLLKEKITALGVLGAMLIILGVVITGSPK
                     NLLKTGKSK"
     gene            complement(542..1366)
                     /locus_tag="PKJ65_07345"
     CDS             complement(542..1366)
                     /locus_tag="PKJ65_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ41685.1"
                     /translation="MEFDGLQRKRDYKTTKKAVIIAITVIILLIPFMMWQNNTLTVTE
                     YDKYSSILPDAFDGYTIIHLSDLHSKNFKGRGAAKVCTLNPDIIVITGDLIDLHNRKL
                     DVAEDTVKMLTNIAQVYFVTGNHEISSPIYPSLLEIFDRYGVTVLDNEQQKIYIGEQS
                     ITIYGYADADAISTDVALDKEHFNLVLCHRPYAPEVFNKVNADLVLCGHAHGGQIRLP
                     FVGGLIAPDQGFFPEYYQGMHDYMDTSVVISRGLGRSILPLRLFNMPEIVVITLHK"
BASE COUNT          436 a          298 c          301 g          484 t
ORIGIN      
        1 tttgtgggag ttgcttttgt tgtattcaac ggaaccttta tcttgaaact caacccgctg
       61 ggagatttgt tggccttctt atcagctctg tcctgggcag tttactctct catcctgaaa
      121 aagatcatta acagatatga caacatactg ctgatacgaa aagtaatgtt ctacgggatt
      181 gtaacgactg ctccgtttct gctcttggaa ggcaagccgt ttgattttgc agccttttcc
      241 gaccctaagc ttattatcag tattttgttt ctcggtgtag tgggaagcgg attgtgttat
      301 atgatatgga atatacctgt aagaacgctc ggaataagga taaccaacag ttatatctat
      361 gccatccctt ttgtgacaat gatattggca gcaacccttt tgaaggaaaa aataaccgca
      421 ctgggcgtct tgggtgcaat gttaataata ttgggagttg ttattacagg ttcaccaaaa
      481 aatcttctta aaacaggaaa aagtaagtaa aaaatcagtt tcacgtgaat gtttaataga
      541 attatttatg aagtgtaata actactattt ccggcatatt gaataatctt aacggcaata
      601 tacttctgcc cagacccctg cttataacta ccgaagtatc catataatca tgcattccct
      661 gataatactc aggaaaaaat ccctgatcgg gagcaatcag acctccgaca aacggaagac
      721 ggatttgccc gccgtgtgca tgaccgcata aaacaaggtc agcatttact ttattgaaaa
      781 cttccggagc ataaggtctg tgacaaagaa ccaggttgaa atgctccttg tccaaggcaa
      841 catcggtact gatagcatcc gcatctgcat atccgtatat agttatgctt tgctctccga
      901 tatatatttt ttgttgctcg ttatccagaa cggttactcc gtatctatcg aatatttcaa
      961 gaagagaagg ataaataggg gagcttattt catgatttcc cgtaacaaaa tatacctggg
     1021 caatattggt aagcatttta acggtgtctt ccgctacatc aagtttcctg ttgtgtaagt
     1081 caataagatc gccggttatc acaataatgt cgggattcag tgtgcatacc tttgctgcac
     1141 ctctgccttt gaaattcttg ctgtgtaaat cggaaaggtg aataattgta taaccgtcaa
     1201 aagcatcggg caagatgctg ctgtatttat catactcggt tactgtcaag gtgttgttct
     1261 gccacatcat gaacggtatc agcaatataa tgaccgttat agctatgata actgccttct
     1321 ttgttgtttt gtaatctctt tttctctgca gtccgtcaaa ttccataccg cgccttattg
     1381 taacctcata tcaataagaa tgcaagcctt ttattagtct tttaattatg ctgcacgata
     1441 tgatatactt cagcatgtag atattatacg aggtagcaaa tgggtaatgt atggttggca
     1501 ttcggactga cacttatag
//
LOCUS       DAOPWC010000387         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_94168_length_1518_cov_2.796309, whole genome shotgun sequence.
ACCESSION   DAOPWC010000387 DAOPWC010000000
VERSION     DAOPWC010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94168_length_1518_cov_2.796309"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKJ65_07350"
     CDS             <1..295
                     /locus_tag="PKJ65_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNZ41686.1"
                     /translation="SLTGRPSDTQFEDPAELIEILLEEYKSTQADPSGGMKEKVAAAL
                     ASAAAIPYGKVLSKMEMEDIIDNLFACKEPNYSPKGKTVISIITMEDIDKRFR"
     gene            372..1115
                     /locus_tag="PKJ65_07355"
     CDS             372..1115
                     /locus_tag="PKJ65_07355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ41687.1"
                     /translation="MNGYGRGLLGLPPVVKNIIFINVLMLLADFTAKSVFAIDLTRIL
                     GLYFPRSEHFMPLQILTHMFMHGNFLHLFFNMFALYMFGGVLERVWGPKRFFIYYMVC
                     GLGAALVHETVIMFQFNKLMNIISPDQLQIVLNDGAAYFKEGRVFTDETMKNMQMLLN
                     VPTVGASGAVFGILLAFGVLFPNTQLVLLFPPIPIKAKYFVLGYGAIELYLSITQPGS
                     NIAHAAHLGGMLFGYLLIRYWRKTTKTLY"
     gene            1115..>1518
                     /locus_tag="PKJ65_07360"
     CDS             1115..>1518
                     /locus_tag="PKJ65_07360"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ41688.1"
                     /translation="MGIKGDIGGIFKKGSTLIRLIYINIAVFIIITLVSIIGFLFNHP
                     EITGKAVDLFAVPAGLQKLLSRPWTIITYMFTHKDIWHILFNMLWLYWFGIIFLEYLD
                     QKKLTAVYILGGICGAIVYILSFNIFPAFTEL"
BASE COUNT          444 a          312 c          317 g          445 t
ORIGIN      
        1 ttccttaaca ggcaggccat ctgatacgca atttgaagat cctgcagaac ttatcgagat
       61 cctgcttgaa gagtacaaaa gcacccaggc tgatccgtca ggaggaatga aagaaaaagt
      121 agctgcagcg ctggcatcgg cagctgccat cccctacggg aaggtactgt caaaaatgga
      181 aatggaagat atcatcgaca atctgtttgc ctgcaaagag ccaaattact ctccaaaggg
      241 aaaaaccgtg ataagcataa tcacaatgga ggatatagac aaaagattca gatgaaaccg
      301 gtttttgccg tattaattga taatactatc tttaaagttg aattttaacg ataaaaacta
      361 aaatcttgcc gatgaacgga tacggtagag gattattggg attgccacct gttgtaaaaa
      421 acataatctt tatcaatgta ttgatgctgc ttgcagattt tactgcaaaa agcgtctttg
      481 cgatcgacct gacgcggata ctggggctgt actttccaag atccgagcat ttcatgcctt
      541 tgcagatact cacccacatg ttcatgcatg gaaactttct gcacctgttc ttcaatatgt
      601 ttgcactata catgttcgga ggcgtactgg agagagtgtg gggcccgaaa agatttttta
      661 tatactacat ggtctgcgga ctgggtgctg ccctggtcca tgaaaccgta ataatgttcc
      721 aattcaacaa gctgatgaac attataagtc cagaccagct tcaaattgtc ctgaatgacg
      781 gtgctgctta ttttaaagaa ggaagagtct ttacagacga aacgatgaaa aacatgcaga
      841 tgttgctgaa tgtgccgact gtaggagcct caggtgcggt ttttggcata cttctggctt
      901 ttggcgttct ttttcccaac acccagcttg tacttctttt cccgccaatc cccataaagg
      961 caaaatattt cgttttgggc tatggtgcca ttgagctata cctttcaatt acacaacccg
     1021 gaagcaatat tgcacatgct gcacaccttg gcggcatgct gtttggctat ctgcttataa
     1081 gatactggag aaagacaacg aaaacccttt actgatggga ataaagggcg atatcggcgg
     1141 aatattcaaa aaaggcagta ctcttatcag actgatttat atcaatattg ctgttttcat
     1201 cattatcaca ctggtatcta taattggatt cctgtttaat caccctgaaa ttactggtaa
     1261 agcagtagat ctgttcgctg tgcctgccgg attacagaag cttctttcaa gaccgtggac
     1321 aataataacc tatatgttca cacacaagga catatggcat atccttttca atatgctctg
     1381 gctatactgg tttggaatta tatttctcga atacctcgat cagaaaaaac ttacggctgt
     1441 atatattctc ggaggcatct gtggtgccat tgtttatata ttgtcattta atatttttcc
     1501 ggctttcacg gaacttat
//
LOCUS       DAOPWC010000388         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_94418_length_1515_cov_3.941781, whole genome shotgun sequence.
ACCESSION   DAOPWC010000388 DAOPWC010000000
VERSION     DAOPWC010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94418_length_1515_cov_3.941781"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..916)
                     /locus_tag="PKJ65_07365"
     CDS             complement(167..916)
                     /locus_tag="PKJ65_07365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ41689.1"
                     /translation="MQNIFIVGANQGIGYYIAKQLLEDGNSVAVMDIQSDNICKLEET
                     YPDNLSVTYADAADENSLNEAIQAAIKRFGVPDIAIHNACICTFKSEPDTDYEIYKKV
                     MNVNYYGALRLAKLVLPEMRKAGAGRIIFTSSGVGVTGFGNISPYASSKGAIEALAKC
                     LDIENETYGISFHIMHPPLTDTASSEGLPIPKEFKADAEKVGRGLAKNINSKKFVICH
                     SMIQKIQMNLCYRHPLRMGKMMWKMTQRAKQ"
     gene            complement(1110..>1515)
                     /locus_tag="PKJ65_07370"
     CDS             complement(1110..>1515)
                     /locus_tag="PKJ65_07370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ41690.1"
                     /translation="SLTIIISAVTALIIYFQVYRSGVIQTLTVPIFLLILLFALTNSF
                     VEEVTFRHTFTSIVEYHNLSPYISKALSLITFGGAHYFGTPGKIPGVLLAGFLAWFLA
                     KSIHETKGFFWAWLIHFIQDIIIMTALFLTLA"
BASE COUNT          443 a          328 c          262 g          482 t
ORIGIN      
        1 ccgttttaac tttctccaaa ctttttcaaa tccatctctc tacctcctta caattacagt
       61 ttagtgaaag ttaaaacggt ttaacaggac atattgcgag caaatgtctt tttgcaagca
      121 tgggacatat catgtcacat tatatcaaat caattttaga gtccggctat tgtttcgccc
      181 tttgagtcat cttccacatc atcttaccca tacgaagagg atgtctgtaa cagagattca
      241 tctgtatttt ctgaatcatt gaatgacaaa tgacaaattt cttcgaatta atattctttg
      301 caagcccccg tcccaccttt tcggcatctg ccttgaactc tttcggtata ggcagtcctt
      361 cagatgaagc cgtatcggta agcggcggat gcattatgtg aaaggatatc ccatatgttt
      421 cattctcaat atccagacat ttggcaagcg cttcaatagc acctttagat gaagcatagg
      481 gcgatatatt cccaaagcca gtcacaccta ctccggaact tgtgaatatt attctccccg
      541 ctcccgcttt tcgcatttcc ggtaaaacaa gttttgccaa ccttaatgcg ccgtaataat
      601 ttacattcat aaccttttta tatatttcat aatctgtatc cggttcgctc ttgaaagtac
      661 agatacaggc attatgaata gcaatatccg gaacaccgaa ccgcttgatt gcagcttgta
      721 ttgcttcatt taatgaattt tcgtctgccg catccgcata cgttaccgac aagttatcgg
      781 gatatgtttc ttccagtttg catatattat cactttgaat atccataaca gctacgctat
      841 ttccgtcttc aagcaactgc tttgcgatat agtatccgat tccctgatta gcgcctacaa
      901 taaagatatt ttgcatacac ttctcctttg ctgtcagttt tttgttaagt taaaccgctc
      961 gcaaaaatac tgatttattt cttatcatca tgatatattt ctttgtattc tctttcaagt
     1021 cataatgttt atctcatttg aataacactg tatcaggcac cgccgaagac aatttataaa
     1081 gctcggtaat aacggaagct tattgccgct tatgccagtg tcagaaacag ggcggtcatg
     1141 ataataatat cctgtataaa atgaataagc catgcccaga agaagccttt tgtctcgtga
     1201 atgctttttg ctaaaaacca agctaaaaat ccggccaaca gaacgcccgg tattttaccc
     1261 ggtgtaccga aataatgggc acctccgaag gttataagcg acagagcttt agaaatatac
     1321 ggactgagat tatggtattc aacgatactt gtaaaggtat gacggaatgt gacctcttcg
     1381 acaaaactgt ttgtgagagc aaataacagt atcagtaaaa aaatcggtac agtgagagtt
     1441 tggattacac cgctacggta aacctgaaag tatatgatta atgcagttac cgcgctgatt
     1501 ataattgtta aactc
//
LOCUS       DAOPWC010000389         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_94826_length_1511_cov_3.643544, whole genome shotgun sequence.
ACCESSION   DAOPWC010000389 DAOPWC010000000
VERSION     DAOPWC010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94826_length_1511_cov_3.643544"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKJ65_07375"
     CDS             complement(<1..425)
                     /locus_tag="PKJ65_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNZ41691.1"
                     /translation="MSEKENSNKKAQQPVEEESGMVRFNKIMADLMEKGKTKGTLVSQ
                     EITDSFDDMEVNSDQMDKLIEMFEEAGIDIVDDEKDLSDEDYETEDIDVSVPDGVSVD
                     DPVRMYLKEIGKVPLLTVEEEIELAKGIEAGDEECKRKLA"
     gene            complement(442..>1511)
                     /locus_tag="PKJ65_07380"
     CDS             complement(442..>1511)
                     /locus_tag="PKJ65_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNZ41692.1"
                     /translation="REDFVIVVEGYMDCISLHQAGFTNVIASLGTSLTDNQAKMIKRV
                     FGQAIIAYDNDAAGQNATLRGLDILAGLGCVVKVLMLPAKDKFGNTIKDPDDFIRLMG
                     KEEFKESLKNALMLVEYKAHLLKQTCDLKTSGGKIAFLKGVAAAIGQVQSPVERDVYI
                     KNISARYNTSYDALLKEVQNRTGDTGRSDTSQTEMPRTKKIASALTDRFTESKLDKLF
                     YTILVLLCNNPTFKGEIEKQLLADNMIHNDFSRDLLQEIINRYDSKESPDEARIAAML
                     DDEQRAAFIKEAAEYHTISDPCKALNDYISKYRHDMIVNNNMVLSRALASDGLSESEK
                     ENIKEELRKNTALLAKYKINT"
BASE COUNT          348 a          384 c          259 g          520 t
ORIGIN      
        1 gcaagctttc tcttacactc ctcatcgccc gcctctatac ctttggcaag ttctatctct
       61 tcttccactg tcaatagagg aaccttacct atctccttca gatacatcct tacggggtca
      121 tcaaccgaca ctccgtccgg tacggagaca tcaatatctt ccgtttcata atcctcatcg
      181 gataggtctt tttcgtcatc cacgatatct atacccgctt cttcaaacat ctctatcaac
      241 ttgtccatct ggtcggaatt tacttccata tcatcaaaac tgtcggttat ttcctgagat
      301 acaagcgtgc ctttggtttt tcctttttcc ataaggtcag ccattatttt attgaatctg
      361 accatcccgc tttcttcttc cacaggctgt tgagcctttt tattggaatt ttctttttca
      421 ctcatctgta ctacctccgg attatgtatt tattttgtat tttgccagca gagcagtgtt
      481 ctttctcagc tcttctttaa tattttcttt ttcgctctca cttaagccgt ccgatgccaa
      541 tgcacgactt aacaccatat tgttgttcac tatcatgtca tgacggtatt tggagatata
      601 gtcgtttaat gctttgcaag gatcggaaat ggtatgatat tccgcagcct cttttataaa
      661 cgcagctctc tgctcatcgt ccaacatagc cgctatcctg gcttcatcgg gactttcctt
      721 cgaatcatat ctgttaataa tctcctgaag aagatctctg ctgaagtcat tgtgtatcat
      781 gttatcggca agcaactgct tttcaatctc ccctttaaat gtcgggttgt tgcaaagaag
      841 caccagaatg gtatagaaga gtttatccag tttgctttcg gtaaacctgt cggtcaatgc
      901 cgaagctatt ttttttgtcc tcggcatctc cgtctgcgag gtatcggacc tgccggtatc
      961 tcccgttctg ttctgtactt cttttaaaag cgcatcataa ctggtattgt atctggcgga
     1021 tatattttta atgtacacat cccgttccac cgggctttgc acctgtccta ttgccgccgc
     1081 aacacctttt aaaaatgcaa tctttccccc ggaagtcttt aagtcgcatg tctgtttgag
     1141 caaatgtgct ttgtattcca caagcataag agcatttttc agcgattctt tgaactcttc
     1201 tttacccata agtctaataa aatcatcggg gtcttttata gtattgccga acttatcttt
     1261 tgcgggaagc ataagtactt ttaccacaca acccagccct gccaatatat ccagtcctct
     1321 taaagtggcg ttctgcccgg cagcatcgtt gtcatacgct attatggcct gcccgaatac
     1381 tctttttatc atctttgcct gattatcggt aagggaagtt cccaatgaag ctatgacatt
     1441 ggtaaagccc gcctgatgaa gacttataca gtccatatat ccttccacga caatgacaaa
     1501 atcctctctg g
//
LOCUS       DAOPWC010000390         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_94985_length_1510_cov_2.276976, whole genome shotgun sequence.
ACCESSION   DAOPWC010000390 DAOPWC010000000
VERSION     DAOPWC010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94985_length_1510_cov_2.276976"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="PKJ65_07385"
     CDS             complement(<1..>1510)
                     /locus_tag="PKJ65_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNZ41693.1"
                     /translation="DTDEYTEEETKNITNTLYTTTLFEGFRRLTGKRTIVMLHAYNSS
                     TSSQMYPTAWAGDFKLGNGALNASLSGHAMVSQDMDNESPEGIHFGFLTPFSIIDSWA
                     YYREPWLYSKANEDITRFYSKLRSSLFPYLYTALRQAHTTGIPMLRPMMLEFQEDMNT
                     AALDKQFMFGEYLLVGTTFAETLKDVNESSIRTENKNTASIYLPKGRWIDYWSGKTVE
                     SKGKWHTSSWPATVGGPLYVKAGAIIAMTSANDSISLNDQSMMIFDCYPDGITSTDIY
                     EDDGESFDYENGRYALTHVQCTESDNDVLIHIGKPTGEYVNKPDKKARLLRIHIEKIP
                     EKVMSGKTVLPMHKEIDRLLCSFEAGWNYDSLMRILTVKPENVWKLHDKQTDLISFYN
                     AETQWKEEPYKSCEIDLCIYKGTLDRIYGTKPYELKLTSRYPVLLCDGQSKTTITASL
                     MDKEGLIVKSDGIKITFEIAGPGVFDNNQSKTEIYAVNGIANVELYSAKTPGT"
BASE COUNT          384 a          313 c          294 g          519 t
ORIGIN      
        1 tgttcctggg gttttggccg aataaagttc cacattggca atgccgttta cagcatatat
       61 ttctgtttta ctttgattgt tatcgaagac accaggtcct gcaatctcaa atgttatttt
      121 tataccgtct gattttacaa tcaacccttc tttgtccatt aatgaggctg taatagtcgt
      181 tttgctttgt ccgtcacaga gcagcaccgg ataacgactg gttaatttta gttcatatgg
      241 ctttgtgcca tatattctgt caagtgtgcc tttatagatg cataggtcaa tttcacagga
      301 tttataaggt tcttctttcc actgcgtttc agcattatag aagctgatca aatcagtctg
      361 tttatcgtgc agtttccaga cattttccgg ttttactgtc aatatacgca ttaagctgtc
      421 ataattccat ccggcttcaa aagaacagag cagtctgtca atttctttat gcatcggtaa
      481 aacagttttg ccagacatta ctttttctgg tattttctct atatggattc ttaacaaacg
      541 ggcttttttg tcgggtttat ttacatattc acctgtcggt ttgccgatat gtatcaatac
      601 atcgttatca gactcagtac attgcacatg tgttaatgca taacggccgt tttcataatc
      661 aaaagattca ccgtcatctt catagatatc cgtactggta ataccgtcag ggtaacaatc
      721 aaatatcatc attgattgat cgtttaatga tattgagtca tttgccgagg tcatagcgat
      781 tattgcgcca gcctttacat acaaaggacc tccgacggtt gcaggccatg aagaagtatg
      841 ccattttcct ttgctttcaa cggtttttcc tgaccagtaa tcaatccatc taccttttgg
      901 gaggtaaatg ctggccgtgt tcttattctc ggtcctgata cttgattcgt ttacatcttt
      961 cagtgtttct gcaaatgtgg tgcctactaa cagatattcg ccgaacataa actgcttatc
     1021 caaagctgcg gtattcatat cctcctgaaa ttcaagcatc atcggacgca gcataggaat
     1081 gcctgtcgta tgcgcctgcc taagcgctgt atacagatag gggaaaagac tgcttcttaa
     1141 tttagaatag aaacgcgtta tatcttcatt agctttggaa taaagccagg gttccctgta
     1201 ataagcccag ctgtctatta tggaaaatgg cgtaagaaat ccaaagtgaa tgccttctgg
     1261 ggattcattg tccatatcct ggcttaccat agcatgtccg gataagcttg cgtttaatgc
     1321 gccgtttccc agtttgaagt ctccagccca ggcggtagga tacatctgag acgatgttga
     1381 tgaattatat gcatgaagca tgacgatggt tcttttacct gtcagtcttc taaatccctc
     1441 aaatagtgtc gtggtataaa gggtattagt gatattcttt gtctcttctt ctgtatattc
     1501 atctgtgtct
//
LOCUS       DAOPWC010000391         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_95144_length_1508_cov_3.068823, whole genome shotgun sequence.
ACCESSION   DAOPWC010000391 DAOPWC010000000
VERSION     DAOPWC010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95144_length_1508_cov_3.068823"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKJ65_07390"
     CDS             <1..525
                     /locus_tag="PKJ65_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ41694.1"
                     /translation="DFEKLRYSYIFQEPRLLDNYTVYKNLQFVLDGKHMSKQQTDENI
                     MSCLKMVEMDKYSHYYPNQLSGGMAQRVSIARAFVKDFDVLLMDEPFKGLDPTQKEDI
                     MERFLEMWRQNRKTVIYVTHNLEEALCLAYDIHVLGDSPTRIIYTSKNAYKMNDSERD
                     IERNRLWEILRSQI"
     gene            498..1457
                     /locus_tag="PKJ65_07395"
     CDS             498..1457
                     /locus_tag="PKJ65_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ41695.1"
                     /translation="MGDIKVTDLATGMEIKKQLFVLWNSEHRKASNNKYYMDFMFKDQ
                     TGEINGKKWDADPNEGSQYETGVLYYITGRVTEFQGNLQLNVSYITKAPEEIQKRIAD
                     FVPSAPFPSEDMYKKALTYIENIKDEQLRRLCYMVYRQYEEQLMYYPAAKVLHHSVRG
                     GFLYHITTMLDVAKALSAVYKEINTDILYTGILLHDIMKLREMDSNELGIVTDYSVEG
                     QLMGHIEIGICYIGEMAKKAGLSENKELLIKHMILSHHNIPEHGSTKMPMFLEAELLH
                     HIDLMDARVYDFNNAYETLEKGAISERIVYSLERRVIRPDFDK"
BASE COUNT          545 a          230 c          345 g          388 t
ORIGIN      
        1 gattttgaaa aactgaggta ttcctacata tttcaggagc ccagattgct ggataattac
       61 acagtgtata aaaacctgca gtttgtattg gacggtaaac atatgtccaa acaacaaacg
      121 gatgagaata taatgtcctg tctaaaaatg gtggaaatgg ataaatattc acattattat
      181 ccaaatcagt taagcggcgg tatggctcaa agagtctcca ttgcaagagc ctttgtaaaa
      241 gatttcgatg tgctccttat ggatgaacct tttaaggggc tggatcctac ccaaaaagaa
      301 gatataatgg aaagattcct ggaaatgtgg agacaaaaca gaaaaaccgt catatatgta
      361 acacataatc tggaggaagc tttgtgtttg gcatatgata tacatgtgtt gggagacagt
      421 ccgacaagaa taatatatac aagcaagaat gcatataaaa tgaatgattc cgaacgggat
      481 attgaaagga acaggctatg ggagatatta aggtcacaga tttagcaacc ggaatggaaa
      541 taaagaagca attgttcgta ttatggaaca gcgagcacag gaaagcatcc aataataagt
      601 actatatgga ctttatgttc aaagatcaaa caggggagat aaacggtaag aaatgggatg
      661 ccgatcccaa tgaagggtca caatatgaga caggagttct gtattatatt accggcagag
      721 taacggagtt tcagggtaat ttacaactga atgtatcata tataaccaaa gcgccggaag
      781 aaatacagaa aagaattgcg gattttgtac cgagtgcacc gtttccgtcg gaagatatgt
      841 acaaaaaagc actgacatat atcgaaaaca taaaggatga gcaactgaga agattatgtt
      901 atatggtcta caggcaatac gaagaacaac tgatgtacta tcccgcagct aaggtgttac
      961 accattcggt aagaggagga tttctctatc atattacaac catgctggat gtggcaaaag
     1021 cattgtcagc cgtgtataaa gagattaata cggacattct ttatacaggc attttgttgc
     1081 atgacataat gaaattgcgg gagatggatt caaatgaatt aggtattgtc acagattatt
     1141 ccgttgaagg gcagttgatg gggcatattg agataggtat atgctatatc ggtgaaatgg
     1201 ctaagaaagc cggcttatcg gagaataaag agttgttgat aaagcatatg atattaagcc
     1261 atcacaacat accggaacac ggaagtacaa aaatgcctat gttcctggaa gcggaattgc
     1321 ttcatcatat cgatctgatg gatgcaagag tttatgattt caataatgca tatgaaacac
     1381 tggaaaaagg tgcaataagc gaaagaattg tttattcact ggaaagaaga gttataaggc
     1441 cggattttga taaataaaaa aagaaggcat aaatccttct tttggtgcgc catcggagga
     1501 tcgaaccc
//
LOCUS       DAOPWC010000392         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_1376
            NODE_95533_length_1504_cov_2.948930, whole genome shotgun sequence.
ACCESSION   DAOPWC010000392 DAOPWC010000000
VERSION     DAOPWC010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854581
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 01:15:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,482
            CDSs (total)                      :: 1,460
            Genes (coding)                    :: 1,449
            CDSs (with protein)               :: 1,449
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 5 of 11
            Pseudo Genes (incomplete)         :: 4 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95533_length_1504_cov_2.948930"
                     /isolate="AMR_MDS_1376"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..563)
                     /gene="coaD"
                     /locus_tag="PKJ65_07400"
     CDS             complement(84..563)
                     /gene="coaD"
                     /locus_tag="PKJ65_07400"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNZ41696.1"
                     /translation="MKIWVCPGSFDPVTKGHIDIISRASVMCDKLIVAVGENKFKKSI
                     FTAEERVDFIRRSLNGLKNVEVESFHGLLIDYARQKKAICIVKGLRAVSDFEYEMQMA
                     FLNKRMDEEIETVFLMTNINYSFLSSSAVKELALHGGPIEGLIPDAIIDDVRKKFFK"
     gene            complement(532..1122)
                     /locus_tag="PKJ65_07405"
     CDS             complement(532..1122)
                     /locus_tag="PKJ65_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNZ41697.1"
                     /translation="MPRIIAGSKKSIILEAPKGQSNVRPTYDRTKENLFNIVASRYPL
                     EGACVLDIFSGTGSIGLECLSRGAKYAVFIDKDISLTRKNIEKCDFSGISRTIRGDWA
                     NGLRVLKEENMKFTHIFLDPPYQSGLHERIIENNDLYDIMSENGVVIAELSDKANVPE
                     STAELVLKDKRKYGICVFAFYIRGELNEDLGMSGEL"
     gene            complement(1124..>1504)
                     /locus_tag="PKJ65_07410"
     CDS             complement(1124..>1504)
                     /locus_tag="PKJ65_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNZ41698.1"
                     /translation="KELKKYGFACSIDDFGAGYSSFKALQTLDFDIIKLDAVFLKINP
                     NNPKKNINLLSGVIGLAKTLGMKVIAEGVETQQEVALLRELKCDVIQGYIYARPMSGT
                     DFITFVKNALSSKTEKEKLAEVIE"
BASE COUNT          393 a          366 c          242 g          503 t
ORIGIN      
        1 gttttgcact tgttacaagg tcttccatct gttcgattat ctcatattcg ttcatagcat
       61 tatccatcac gttacccctc acattattta aagaattttt tcctaacatc atctattata
      121 gcatcaggta taagcccttc aatggggcct ccgtgtaaag ccagctcctt gaccgcagac
      181 gaactcaaga aagaataatt gatgttggtc ataagaaata ccgtttcaat ttcctcgtcc
      241 atgcgtttat tcaaaaaagc catctgcatt tcatactcga aatccgatac ggcacgtaaa
      301 ccttttacta tgcatatagc tttcttctgc cttgcataat caattaacaa cccgtgaaaa
      361 ctttccactt ccacgttttt caatccgttc aaagagcgcc ttatgaagtc caccctttct
      421 tcagccgtaa atatgctttt cttgaatttg ttttcaccca ctgcgacaat aagtttgtca
      481 cacattacac tggcccttga tattatatct atgtgtcctt tggtgacggg atcaaagctc
      541 cccggacata cccaaatctt cattaagttc tccccttata taaaatgcga atacgcagat
      601 tccatacttt cgtttgtctt taagaacaag ttcggcggta ctttccggta catttgcctt
      661 gtccgacagc tctgcaatta ccacaccgtt ttcactcatt atatcataca aatcattgtt
      721 ttcaataatt ctttcatgca gtcccgattg gtacggaggg tccagaaaaa tgtgcgtaaa
      781 cttcatattt tcctctttta atactcttaa tccgttcgcc caatcacctc ttatggtacg
      841 tgaaataccg gagaaatcgc acttctcgat atttttcctt gtcagtgaaa tatccttgtc
      901 tataaatacc gcatactttg cacctctgga caggcattcc agtcctatgc ttcccgttcc
      961 ggaaaaaata tccagaacac aggcaccttc cagaggatat ctcgatgcta ctatattaaa
     1021 cagattttct tttgttctgt catatgtcgg gcgcacatta ctttgacctt tcggggcttc
     1081 cagtataatg ctttttttgc ttcccgctat tattctcggc atgttattca ataacctctg
     1141 ccagtttttc tttttccgtt ttggaactca gagcattttt aacgaatgtg ataaagtcgg
     1201 taccggacat gggacgtgca tatatatatc cctgaataac atcacacttc agctctcgta
     1261 ataaggcaac ttcctgttga gtttccaccc cttcggctat taccttcatt cccaaggttt
     1321 ttgcaagacc gataacaccc gagagaagat tgatattttt cttaggattg ttggggttga
     1381 ttttcaggaa tacggcatcc agctttatta tgtcgaaatc cagagtctgt aaggctttaa
     1441 acgacgaata tcccgcaccg aagtcatcaa ttgagcatgc gaaaccgtat ttcttcagtt
     1501 cctt
//
